LOCUS       DAORQN010000001        65404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_27_length_65404_cov_38.285345, whole genome shotgun sequence.
ACCESSION   DAORQN010000001 DAORQN010000000
VERSION     DAORQN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 65404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_65404_cov_38.285345"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK614_00005"
     CDS             <1..191
                     /locus_tag="PK614_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80630.1"
                     /translation="GASAGLIGGPTGIAIGAASGGAVGGVLGFLAGTYKPLQPQPDYA
                     AFVERCLKEKGYETTGWQ"
     gene            complement(232..1467)
                     /locus_tag="PK614_00010"
     CDS             complement(232..1467)
                     /locus_tag="PK614_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRB80631.1"
                     /translation="MPPGRNPAVALMLSALSLLLGLATTGCLRTMEAIDPRSLPITST
                     SGQRLQHHVSFLASPELSGRQPGSPGNRQAARYIEDQFRAVGLEPLPSLGGYRQAISP
                     TIGDNLLGFIPPTAGSAPAQWMVIGAHYDHLGYPFLGADDNASSIAILIETARHMAGL
                     PQHGILFVAFNSEESPYFGKAEMGSQFLFHHLPPEIGRAENLQAVVIMDLMGGVQWPP
                     IKDAIFAAGAEKSPALYQRVKQASAPSLTVFPVGMHLVEEIPEIGHKAFSDYDVFRNH
                     GLPFLFLSAGRTPRYHTAGDVTSTLHYDRMAATVEWLRHLIALIGQDEAPYGFQADRV
                     EWADEVVSFREIVNRAVQDETRIPGTSRWSLRKLRQDAEWLRDADRSAPTPQDRDRLE
                     RISIRVQCLMADYSGCFTF"
     gene            complement(1476..1922)
                     /locus_tag="PK614_00015"
     CDS             complement(1476..1922)
                     /locus_tag="PK614_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRB80632.1"
                     /translation="MAEPSKESLDKMWKYVKGFAEKSGTAMHPNEAVTNAVVLGLAAH
                     VDELGKPLCPCNFYPDKQAEAKLRRWMCACDEMQMYKYCHCLLFVREDGMPITEYLPE
                     DHEGRQCYGLITDPTPEKGRALRHKALPMAPKETTPPTTPESAPQP"
     gene            2060..5095
                     /locus_tag="PK614_00020"
     CDS             2060..5095
                     /locus_tag="PK614_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRB80633.1"
                     /translation="MASGRSSEQLCDLLAEQLGPDAAAAVVKAIADSAARPDALDGVL
                     VLLDELTEEAPKAAKAAIDALADMQRRGLLADVLPWVDLGVAIAADSGALALRFFKES
                     PLLLGLLEPSSRAIVLAAGLELADRQANVAVEFIRIAPEAVAVLSPADWPVWMDLACE
                     LTETDFALAVEYIRQIPSIARVLSLDAVRAWVRFGMKLIVENSLGKPDYLGVLEFFRT
                     SPAILEDIPGPSHRNQVIGIGAQMAERSPASGIAVLAEAPAMLRRLPSEEWRTQVLRY
                     GLLVAERDAETALAYLRRCPELIALIGSGEGAQEKFQSWFASGMEVLEYSLEGGRAYF
                     AMETEKALGAVSQAMSGVPLRQIARRVKLFAQALCGRDVRIHDLPDSLESTQPMARAT
                     VSEDGQSIGLPSLVRRYPTYDENVRLYMVMAAHEAGHLEFGTFDLPIGRLQDLVEDLA
                     QRYGRAVPNEARTLGDVFARYPQPGLIRDLWALVEDARVEYLLRAEYPGLSRDLASVA
                     KDAVNVRSLAHGMTVRELVVDQLLLFSTTDAGTVIVPDAVKSEVDQLWSLCRTIYHPR
                     ATAEEAVRLADRLYVRMDELLAIRRESIPSQDETEVLEQSIPAPKSSEDLSDTYRPMD
                     NWDYRGAMDPNLVRDRSESSPEQSAASGQGDSEGGAGLAGDAGGSSGAGTARSQQMSQ
                     DVLVPGRRKPSLVEEVLAVEGEGARPEDETAGAVKAVRYREWDAAIQDYRMNWCRVVE
                     REAIEGTSDFVEETLAAQRGTVTLLRRYFESLRPPGLRRIPGQLDGEDLDMDAVIGRM
                     ADLSAGIEPSERIYVRREKREREVAAAFLVDLSGSTSRQVEQGRRIIDIEKQGLVLLC
                     EALTAIGDQFAVYGYSGQGRNQVDFVVVKGFDELVTGRAGAKLGGIVPLQQNRDGAAI
                     RHATSKLLARQAKTRILVVISDGRPLDDGYKDDYSLEDTRMALREARASGIEPVCITI
                     DKQTDPYLRRMYGDVRYVVIDRVEGLPEKLPRIYHRLTA"
     gene            5166..5549
                     /locus_tag="PK614_00025"
     CDS             5166..5549
                     /locus_tag="PK614_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80634.1"
                     /translation="MPPWLYKLFTGHQYPYVRRQAKFANRDTKPGEERPEPTREEIDA
                     KFWEIYPRCTAKILQEVKAGMIVVFHELGEYPAGGYQGLVDAPEEFLAAHYGKKKIKV
                     NFYDGDNFVCTINFKVGGWTGHEHA"
     gene            5618..6562
                     /gene="mdh"
                     /locus_tag="PK614_00030"
     CDS             5618..6562
                     /gene="mdh"
                     /locus_tag="PK614_00030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRB80635.1"
                     /translation="MMGRPKITVVGAGNVGGTVAQRLAEKNAYDVVLVDIVPGIPQGK
                     ALDITQAGPVCGYSTRVVGTNGYDETAGSSIAVITSGMPRKPGMSRDELLATNAKIVK
                     TVVRELVSRSPNIILILVTNPLDAMVHVARQVSGLPKSRVLGMAGVLDTARLRSFVAE
                     ELNVPGTEVQAMVLGGHGDTMVPLVRQTTVAGKPITDRLSPERLAALIKRTQDGGAEI
                     VGLLKTGSAFYAPSASAVDMVEAIMNDQKRVVPCAMFCEGEYGLKDVIVGVPVTLGRS
                     GGESIVEYELTAEERTALQASADAVRDLCAVVDRLLTA"
     gene            6662..7249
                     /locus_tag="PK614_00035"
     CDS             6662..7249
                     /locus_tag="PK614_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRB80636.1"
                     /translation="MKFLEDPYQTLGAGFALAVGLIVVYLGMAGVGAGDADWFGLIVR
                     WIHFLAGITWIGLLYFFNLINAGFLKSLDGPTKNIVIPKLMPAALNWFRHGATVTVLA
                     GIVLYGYLYSKGGTGALALGIGGLLGIIMMGNVHGIIWPNQKKVIAAVAAAAQGTPAP
                     PEMAQWGKTALIASRINFLLSIPMLFFMGAGSHLK"
     gene            7503..8804
                     /gene="nuoF"
                     /locus_tag="PK614_00040"
     CDS             7503..8804
                     /gene="nuoF"
                     /locus_tag="PK614_00040"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRB80637.1"
                     /translation="MTLPQPRVLQKLEGAPWEIDPYLKTGGYEAWRKCLKELTPNDIV
                     GEIKKAGLRGRGGAGFPTGTKWDKVLNHRVQERYFVCNAGEHEPGTFKDRHLLKHIPH
                     QLIEGCLIASRTVNAKASYIYVNHEYAEEEANLRKAIAQARERGLLGKNILGTGVDLD
                     LEVYSGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCNI
                     PLILKNGAAWFTQVGTEKCPGTMLFSLSGAVNRPGVYEMPMGVTIRDLIETCGGGVPN
                     GRKIKAVFPGGPAFSMVTADQLDLPMDFDSLKKAGTGLGSAGTIVIDDATCMVAATLK
                     YSNFFKGESCGQCPPCRMGTINLATLMDKIERGEGSQKDLDSLLQLCGFVKGTGYCTL
                     ITGAAVLVQSSLRLFQHEFEEHIRLQRCPFQPARTGAGANA"
     gene            8817..9173
                     /locus_tag="PK614_00045"
     CDS             8817..9173
                     /locus_tag="PK614_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB80638.1"
                     /translation="MPRVSFLHPQGRSGEVESNLTLLEAAKTLGFELNHDCGGNASCT
                     TCRVEVQMGGDNLSEIDFDEQDLLDREALSEPWHRLGCQARVLGDVVVRVPETKWVQP
                     TSASSGEVGKRGAGLP"
     gene            9237..9557
                     /gene="erpA"
                     /locus_tag="PK614_00050"
     CDS             9237..9557
                     /gene="erpA"
                     /locus_tag="PK614_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRB80639.1"
                     /translation="MVTITQLAEQKIKELMTEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMADDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSLQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            9690..10139
                     /locus_tag="PK614_00055"
     CDS             9690..10139
                     /locus_tag="PK614_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRB80640.1"
                     /translation="MSQATITRRYRFCAAHRLHTDQLSAEENWATFGKCNNPNGHGHN
                     YVVFVSVKGDIDPVTHQVLDVSRLDAVVERIVVQRFDHQDLNLDPEFATQTTTGENLV
                     LLIWDLLVDKLPAGRLVKVGVIETRDNYFEYSGSVESKRREEGVRHG"
     gene            10132..10767
                     /gene="folE"
                     /locus_tag="PK614_00060"
     CDS             10132..10767
                     /gene="folE"
                     /locus_tag="PK614_00060"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRB80641.1"
                     /translation="MAKVVSGARARRSRVSAAPTDEDPIEGLMTNLLLELGEDPDRNG
                     LLNTPKRVAKAMRFMTQGYRQDIDHLLNGALFPIEYDEMVIVKDIDFFSMCEHHLLPF
                     FGKCHVGYLPNKKVVGLSKIPRVVDAFSRRLQVQERLTLQIAETLKKKLNAHGVAVVM
                     EARHLCMMMRGVEKQNTVAVSSSMLGVFRTQQQTREEFLKLIRGSSVRDGN"
     gene            complement(10764..11567)
                     /locus_tag="PK614_00065"
     CDS             complement(10764..11567)
                     /locus_tag="PK614_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB80642.1"
                     /translation="MFCDTKGIRLAYDEQGTGLPLVFLHAFPLNRSMWTPQTTALSRQ
                     FRTIAVDLRGHGESDAPLWAFSLEDYADDVCALLDHLAIPQAVLVGLSMGGYVGFAFS
                     RKYGNRLKGLVLADTRAQADSPEGRTGRFNLAQTAYGKGTDAVADIMLPKLLGATSLQ
                     QKPELVDYVRRTIRHTPVSGILVDLMAMANRPDSVAHLRTLACPTLVVVGQEDHTTPL
                     ADAQVMATGIPGARLAVIPAAGHLSNLEQPEVFNDLVRNFVEELRAAGD"
     gene            11723..13012
                     /locus_tag="PK614_00070"
     CDS             11723..13012
                     /locus_tag="PK614_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRB80643.1"
                     /translation="MSSHDSTGDLFAPAQTATRPGKVPLAERMRPRSFDDLVGQDEVV
                     GPGRLLRNAIERDQLSSVIFWGPPGCGKTTLAGLVAQHTESQFVPFSAVTGGIPELRE
                     IIKAAEHRRAMGRATTLFVDEIHRFNKAQQDAFLPHVERGTVVLIGATTENPSFELIA
                     PLLSRSIVVLLKPLTEESLGSILDRAVADSERGLGSLRITLHPTAREQLIAFGNGDAR
                     SLLTTVEFVAGQAPVGPDGSRIIDERVLEAALLKKALRYDKSGEEHYNLVSAYIKSLR
                     DSDPDGALYWLARMLEGGENPRFIARRMVIFASEDIGNADPMALVMANAVAQAVEFVG
                     LPEAQINLAHGTTYLASRLKDNASYVGLQEAVRDARQHGNLGVPLHLRNAVTSLMKDI
                     GYGKGYRYVHDDPAAKTDQNHLPESLRGTRYYRPRTP"
     gene            13075..13452
                     /locus_tag="PK614_00075"
     CDS             13075..13452
                     /locus_tag="PK614_00075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB80644.1"
                     /translation="MARQSSSAESVSERRKYVRATLVGSALVSPQSGAKGFTAVLNNV
                     NKIGAGLHSKETLPIGAKITVSLAFLDPDRVEQQEKLNATVAWAKPWEKGILLGVVWD
                     DLVTKEKNRWLYYYLEETLKSTD"
     gene            complement(13449..15290)
                     /locus_tag="PK614_00080"
     CDS             complement(13449..15290)
                     /locus_tag="PK614_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB80645.1"
                     /translation="MLQLESVHKQFATKVLLEGATAHLRPGARVGLVGPNGAGKTTLF
                     RMILGEESPDKGTIRKRPRLRVGYLPQELETIVGKTVLDATHRDLYPEHEAERILAGL
                     GFSEADFSRPIENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFERFLLD
                     SNLTLLVISHDTKFLDGIATHIWELRDRTIQEFRGNYTKFQELRAARDAQIESAANRQ
                     SKEVARVQNFIDRFRYQANKAKQVQSRIKQLDKVKLIERQRDTKRVRFKFPLPSASGR
                     HVLELKGVAKSYGEKVIYRSLDFTVERGQRIALVGENGAGKSTLLKILAGVLPFEKGT
                     RQVGHGVTLHYFAQHQAESLDPDDTILESLAEVSAQAETNFLRGIAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDMLTDALTDFPGTIIFIS
                     HDPTFLTRVATLIVEVDEGQAKNYLGDYEYFLWKKAQEFESIKESSAELAAAQAGKSD
                     GPTKAMASQVQQKSQGGERRDLSKSQARLEKQVSRAESEIASMEAKVKARELELADPA
                     LYQELGRWSELQREQEGWKKELERLTARWESLSDELQGVREKLTAIG"
     gene            complement(15449..15524)
                     /locus_tag="PK614_00085"
     tRNA            complement(15449..15524)
                     /locus_tag="PK614_00085"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15489..15491),aa:Ala,seq:ggc)
     gene            15884..17206
                     /locus_tag="PK614_00090"
     CDS             15884..17206
                     /locus_tag="PK614_00090"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRB80646.1"
                     /translation="MANLVIIGSQWGDEGKGKIVDILAKDADMVVRYQGGSNAGHTVI
                     NKRDTFIFHLIPSGILYRGTRCLIGNGVVVDPGALIEEMDHLQGQGVKIGKNFAVSDR
                     AHLILPYHKAIDKASEQSKGVRRIGTTGRGIGPSYGDKMARIGIRMGDLLNPPLFKQK
                     LEENLVDINWLLEQLHKVDCFELEKVYQQYMGYADRLKSYIIDTALVVNKAVDAGKTV
                     LFEGAQGTHLDVDFGTYPYVTSSSSSAGGACTGTGVGPTKIDAVMGITKAYTTRVGSG
                     PFPTELTDEVGGWLQERGKEFGATTGRARRCGWFDSVVVRHATRVNGLSSLAVTKLDV
                     LDGCKELKVCTGYRVNGKIHREMPSDLLALTNCEPVYERVRGWSAPTTGVTTYKNLPA
                     EAKRYLTRIEELAECRIDMISTGSRRDETIILNNPLKAGRRVRLQRSR"
     gene            17320..17395
                     /locus_tag="PK614_00095"
     tRNA            17320..17395
                     /locus_tag="PK614_00095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17353..17355,aa:Lys,seq:ttt)
     gene            18055..18459
                     /locus_tag="PK614_00100"
     CDS             18055..18459
                     /locus_tag="PK614_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80647.1"
                     /translation="MFQWVSIQFLITNGDIENPIEDLPFTTDGAGRGPFVKTSFHIFQ
                     AIFRSDRCERLMPEAMTTGEVVSDTGMAGQNVILKNSFWSRAIDALFDLESLPIQRED
                     IAEGDERTRLLVFFVLNLVNQVNGTLLCLLGR"
     gene            complement(18564..18845)
                     /locus_tag="PK614_00105"
     CDS             complement(18564..18845)
                     /locus_tag="PK614_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB80648.1"
                     /translation="MNQERQASATLEIMTLAEAAVYLRVSKSTLYQRKDIPRHRMPGS
                     REIRFLKDELIAWLKSGDGRAQSADSSELVLPLDTAHTTVYHRNPKYRG"
     gene            19069..19572
                     /locus_tag="PK614_00110"
     CDS             19069..19572
                     /locus_tag="PK614_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRB80649.1"
                     /translation="MSVDSSLGSSNGGRPVSPVRKYGIPRYRVTLVREGRALPAAESV
                     HTSEGAVAILRPLFSGLDREQFLICGLDAKHKLIGINVVSTGSLNLTIVHPREVFKPL
                     ILMNAGAWLCSHNHPSGDITPSPEDRILTKRLREAGELFGITLLDHLILAEERYYSFA
                     DQGWPGA"
     gene            complement(19569..19697)
                     /locus_tag="PK614_00115"
     CDS             complement(19569..19697)
                     /locus_tag="PK614_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80650.1"
                     /translation="MTAQQIQTIGNALAVDLVAWGTAAIGLALVAAGAAWVLRLLR"
     gene            complement(19788..20000)
                     /locus_tag="PK614_00120"
     CDS             complement(19788..20000)
                     /locus_tag="PK614_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80651.1"
                     /translation="MKGWGWVKGLGGLPMAFVFALGVPALSQAQLFPVSADVATVRAD
                     LLLWATALIGVALAIYAFKRVRAIVG"
     gene            complement(20161..21168)
                     /locus_tag="PK614_00125"
     CDS             complement(20161..21168)
                     /locus_tag="PK614_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HRB80652.1"
                     /translation="MIELYEGVPGSGKSYHAICEKFLPWVKQGRRLYIAVDGIYLDRL
                     SLFTGIELTILEKQITLWKDSVEVLQAFAHVEPGSGVIIDEAQTVFRSMQKVEPGLLR
                     WLETHRHYGVDILLMSQDFRQMSQGVTRLIEATVKFRKLAFVGLSKKYQGKVRGNPED
                     HEVIRAFVGTYSPAIYSYYSSYASAAIREEKRSHTVFKSARVAIGIAAGLFAIGLMVW
                     RPWSSLSASKASSVAGSTPVPTTTIGAPASGSASLLNPLGMSQPAPATPAVPKRPVRI
                     LGGAGMTKDRQGWRYLLDSGEILTAAQITGRYGLSVSEVYEEGSMRLIGEGVFYGPAG
                     D"
     gene            complement(21248..21523)
                     /locus_tag="PK614_00130"
     CDS             complement(21248..21523)
                     /locus_tag="PK614_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HRB80653.1"
                     /translation="MTAILTLIYCWLQEFFFSLTDWGLGIWDSLLSVADSTLATIGTA
                     GLTLPVIPDQYAWVLGATGMSQALAIVASAMGTRFILQTIPFVRWGS"
     gene            complement(21520..22788)
                     /locus_tag="PK614_00135"
     CDS             complement(21520..22788)
                     /locus_tag="PK614_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80654.1"
                     /translation="MKVLSLMTYLGFVWALLVSFCVAPGEAFSQPATQYSRVVAQAER
                     IAYLAAQRSALASQVATAAVAPSAASMAVRMVAGPVGWAALGVSAGLVLAQMYYSQTD
                     LSAVKTAASTPGGWQVASTNAGTQTFPGFGTNTPGNPAYPNATIQFSATNVPLCAVDT
                     EYQHDWVVGPFQSLSTAVFFTGNFFVNGPAVGGYTLYVCHRKGIPGSTSPIQEGLIPP
                     TQQQVANFVGGLPASDPKSVEAHTNPVGTTGTTQPADTTISQAVTPTEMPTTVKPKPV
                     PAGDIVVVDTVPPPASTPQQNTQQQTTTTTTTTTQNPDGSTTQHEETQATTSCAVGSH
                     ESRTFGTVLQAHQTTWEMSGLLGTLNLLKSLTWPSTLPVIALPSAFFGSQQVDFNQWA
                     WFFTVLRTLVIATASIAAYRIIFVGGGQTT"
     gene            complement(22880..23143)
                     /locus_tag="PK614_00140"
     CDS             complement(22880..23143)
                     /locus_tag="PK614_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80655.1"
                     /translation="MQVKAEGAVQGYVERRSREGKVYRSVDFYVKGKDPGVLRLGIPE
                     DQMPLIEVCKQAEGKQARASIEVRKFEQTGRVFFDLSGLEVLK"
     gene            complement(23344..24336)
                     /locus_tag="PK614_00145"
     CDS             complement(23344..24336)
                     /locus_tag="PK614_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB80656.1"
                     /translation="MNGSGGFTQTIDWLAFTLPKAEVAEVIALIGGDWFQSETGFRGY
                     PVAQLMTEGKTGVGKLGTGAPRNPKEVHVDLSAGIVSQWDETKLKSVLQWIFSQKGHV
                     TRIDVALDDREASVAVETVRLAVEAGQLVSRSKQFKVIQASNHREGVRTGETLYFGSR
                     ESQSMLRVYDKRLELQAKGREDAGLYGVRWELEFKQDRAQACAKALLTLDREDWRAFL
                     VGVLRSYVDFRETTREAESYEKYRAPLVGWWKALTEGFMRCRLVVERIQQRLDDVAAW
                     LANAISPMLAVVVACRGDQFLFEMIYAGTKRWTQKHYALLKQRRRGTPYVLRVS"
     gene            complement(24333..24641)
                     /locus_tag="PK614_00150"
     CDS             complement(24333..24641)
                     /locus_tag="PK614_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB80657.1"
                     /translation="MKTSWLTADELAAVLKVSVKSIRRAYRKGEIPVERICRFVRFDL
                     QRVKEAMQGNGQGQSPFSSRTQGQRSATGGASRRRAQRTSPRLGKTGASIAQKPRGKK
                     "
     gene            25550..26164
                     /locus_tag="PK614_00155"
     CDS             25550..26164
                     /locus_tag="PK614_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HRB80658.1"
                     /translation="MMVEALAEKHYGAELTAGALKVPESRIIADLLLRGVAPEEWHET
                     LYKQNALQTRNLETARRLGRLIRQRLELMNVDLWRLVRDGSKTVATHACLAAAIKHSA
                     LLGDFLDLVVREQYRLFASKLSPTLWEEYLNDCRGRDPEMPLWNDSTRRRLRSSVYQT
                     LAQAGFLDSTRTLRLQPVHIASEVLSYLRDHREEYVLRCIQVSP"
     gene            26161..26775
                     /locus_tag="PK614_00160"
     CDS             26161..26775
                     /locus_tag="PK614_00160"
                     /inference="COORDINATES: protein motif:HMM:NF020329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HRB80659.1"
                     /translation="MSDLLNDRLNKILPRIISDDFLSGSGIGNEISFYIFDYPPEEEL
                     RVRAHLHFLEDHIPKQKPELRFTHINLFDLVLDHLKRRKLLEKALQIQREKGDEALKA
                     ALAGPLHPQKLASIFCEVARPDQNKLVLVSGVGSVYPLLRTSGLLSNLQNVMGPIPLV
                     LFYPGKYDQLTLRLFGKLSLSAHLDGMSKSKKPEHYYRAFKLVP"
     gene            26788..30363
                     /gene="brxC"
                     /locus_tag="PK614_00165"
     CDS             26788..30363
                     /gene="brxC"
                     /locus_tag="PK614_00165"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRB80660.1"
                     /translation="MKIQNLFARDLFRSINGVVKADQLDESSVWQELDEFVVTRELDQ
                     HLRRFFSTYGDAIKHPQDPDVAGKIGVWVSGFFGSGKSHFIKVLSYLLQNKTHTYQAQ
                     SKRAVEFFESKIKDAMLFGDIKRAVASNTDVILFNIDSKADHRIGRDAILSVFLKVLN
                     EMQGYCGDHPQIAHLERHLEGNGKLAQFHDAFREASGSEWVANRDAYHFHRDEVVKAL
                     GETLKMSKESAEKWVDGAEGSFALTVENLCKWVKEYLDSKGEEHRLIFLVDEVGQFIG
                     TDSHLMLNLQTITEELGTICKGRAWVVVTSQEDIDAVLGEMKQSKANDFSKIQGRFRT
                     RLSLSSANVDEVIQSRLLAKREEVKSNLQEVFRKKGDILKNQLTFTNCGMTFKSIKDG
                     EDFVQNYPFVPYQFQLVQKIFEAIRRAGATGLHLSRGERSILDAFQSAGKQVALEEVG
                     ILVPLYRFYPSIESFLDTVVKKTIDQAKDNPSLEHPFDIHLLQVLFLIRYVEEIKGNV
                     DNLVTLCITEIDADRLALRRKIEGSLQRLEKETLINRSGDLYFFLTNEERDINREIKN
                     VELGSGEEAKLLGELIYNEVLKEQRKYRYPDNKKDLEFNRFCDLLPIGNRFDGALQFS
                     VITPLNDEYELYDNAKCLLDSSPEGGSILVRFGNDETLGRELRTYLQTEKYVRHKNDG
                     TLPESAKRILRDISGNNQDRRARLVTLLGEMFAGADFYAAGQPLKLKASTPLAALDES
                     LGYLVKNTFNKMGYLKRLSQEPLKEIQAIIRSNDIGQQTLLLETEEGNKQAIEDLRNY
                     VALMSSNNKQIVLQDMIDKRYSLRPYGWPEDEVVMLIARLIVLSEISLMMDGTLIPLE
                     KAYDAITTPAKRRKIVILKRQTTDPKAIQGARTLGKELFHEMGPDGEDALFAFLQKKL
                     QSWQSSLTSYKPLAETGDYPGRDQIADGLMRVKKLLTSDNSYKFIQYLNEQKNELLDF
                     ADHFTNLEQFYEHQRPTWEKLRKAYGRFQRNHLELERDAKAGVALKRMQQILNASSPY
                     SLIKETEGLIATVSAVNSALVATARQQALVKIDGHLATILKDIEVAMGDPQLRDACMR
                     PLEALKSTVQLEESLAHITQIETEALKEFDIASSRIEESVGRASEQSKEEQPKPVLRK
                     KRVVEPAKLVASPYLETQADVEAFLKTLRSELEQALTKDERIEIR"
     gene            30387..33911
                     /gene="pglX"
                     /locus_tag="PK614_00170"
     CDS             30387..33911
                     /gene="pglX"
                     /locus_tag="PK614_00170"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HRB80661.1"
                     /translation="MNRTKLKIYAPQARRDFIQAVADRAAFYGLTDKKIEPITENGDM
                     AIIGGRAFPRSIAAKRKVLEERIAQYGFEQTTDAIAYTWFNRLVAIRYMELHGYLDHG
                     YRVLSHPDASKSIPEILEQAEHLDLAGLDKHKVIELKLEGSKEAELYRMLLLAQCNAL
                     HKTMPFLFEWIDDETELLLPDNLLHTGSLIRKLVTDIDEEDWQEVEIIGWLYQFYIAE
                     RKSEVMARKSAVPTEDIPAVTQLFTPHWIVRYLVENSLGRLWLLNRPSSRLREHMPYY
                     IEGEAETDFLRINRPDDIRLLDPACGSGHMLTYAFDLLFRIYEEEGYVPNEIPGLILK
                     HNLNGLEICPRAAQLAELALVFKARAKSRRFFQPGTLVQPRILALQDIRFDEGELRDY
                     IAALDLGALFKEPMLRLLHQFEDATTFGSLIQPCLDEQNITFARHAIEAKDLGGQLFL
                     RETHRKVLRALEQAVMLGKRHHIVVANPPYMGHLAFNSSLKALAKACFPLSKENLYSM
                     FMQRSLELTISDGFCALVTMNSWMFLVKFVELRVSLLKSVTIECMAHFGPRAFDSIGG
                     EVVQTTAFIFLARSIPTFRGSYQRLIDGKSEAEKNEALLKRAAIYRVSSQELLLVPGA
                     PITYWISERTRQIFASDKPLEAVAEIRQGMATTDNKRFLRIWSEISFSSISFGNISRN
                     QASESGKSWFPYNKGGEFRKWYGNNEYIVLWQNDGQVMFDLVRKKYPRISDPEFVIKN
                     PNTYFQESITWSGISTSSFGARYSPPGFIYDVVGSSVFPHNKDYFTLLSLFCSKIAAH
                     FVQILNPTYHFQVGDVQRIPWEPEAIERCQDTTSLVAREAVRVAKSDWDNFETSWDFT
                     ELPLLRVGTKGTTLSASWQAWSDQCTAAIHRMRDLERENNRLFIDAYGLADELDPDVR
                     EDEITVSRSDQPKDVAAFLSYAIGCMMGRYSLDKPGLILADAGDTLEHYLTKVGRPKE
                     QLSFTPDEDGIIPVLDREWFADDILARTEAFLCATFREATREANLKFIEESLGKDLRK
                     YLLTDFYKDHLQTYKKRPIYWLFSSGKERAFQCLVYLHRYQDGTLARMRTEYVIPLQG
                     KMAARIEQLASETHEAASTSQRKALEKEREKLLKHQAELRTFDEKLRHYADQRITLDL
                     DDGVKVNYGKFGDLLAEVKAVTGGTDED"
     gene            33911..36541
                     /gene="pglZ"
                     /locus_tag="PK614_00175"
     CDS             33911..36541
                     /gene="pglZ"
                     /locus_tag="PK614_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HRB80662.1"
                     /translation="MDTRQIHDTLHRMYKEEGARIVFWNDPDKEFQTILPALNLDGVN
                     IIQLNGVGALEVKIRVEREDPTGRYLLYSPSEEPDYENDWLLDIRLYSRPFRADRASI
                     LLNELGLKNLHVRQHLADRRKFFDNKERLQKLKNLVEPNDIASDLDRKMIATVVKADQ
                     PEWFNIIRTLYHAYTDNGDEIDLQTEPAAWEQIEKFELDKPFWEMANTTFGYSEATPT
                     LKNLLLRLFATDYVHHLKAAPPNALVHLILPAQGRSNAVVCLGQWRDSSSKGSSYDRL
                     SEEVADLIHLEDYLRGLEIPDLLDVMTFLVVERGIASRLRERVHQTADMINPDEVRSI
                     ATRRQAGHWASLMVNGSPAVPRRALHAVYHALVAAADFFGLRNQHRDGFVFHDPAALY
                     RAYEDELFRFDQLYRHFCEAADHAEAQGWNILKPLRELMEDCYANWYLATLALAWGKF
                     LDPQGIAQLLNKWQLEQIPNQQDFFNRQVRSRLEEAENRKAYVIISDAFRYEAAQELT
                     QELNGTYRFEATLTSQLGVVPSYTALGMASLLPHQSLSYKQNGDVWVDGKPTSSTEQR
                     GAILESVEGIVCKADELVAMKKEQGREFVAGKRVVYIYHNVVDAIGDAAATEEKTFQG
                     VRKAIEELAALVTYIINNLNGNHVLITADHGFLFTESPPGQPDKTKLDQKPEGTVRSK
                     KRYVIGRKLPDNDYVWHGKTSVTACAEGEMEYWIPKAGNRFHFTGGARFIHGGAMLQE
                     IVVPVITVKHKKDKSTREDTKVKSVIVHVLGASHRVTTNRHRFELIQAEPVSERVKPI
                     TLKVAIYEGHSAVTNVETVTFDSASDKVDERKKWVHLVLKDHQYHKSTAYGLILRDAD
                     TGIEQQSVPVTIDRVFIDDF"
     gene            36531..38579
                     /gene="brxL"
                     /locus_tag="PK614_00180"
     CDS             36531..38579
                     /gene="brxL"
                     /locus_tag="PK614_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HRB80663.1"
                     /translation="MTFENKAIDTNLDDLLNRHFAGKVVRKDLTKLVKEGANVPVYVL
                     EYLLGTYCASTDETIIKEGLVTVKNILAENYVRPDEAEKVKSTIRERGSLKIIDKITV
                     TLNEKRDVYEALLANLGVKGVEVSTSYVKKFEKLLVGGIWCIVTLQYFYEENQKGSPF
                     VIQDLKPIQMPNMDLEELFQGRRAFTEEQWIDVLLRSTGMEPTNFSERVKWHLLARMI
                     PLVENNYNICELGPRGTGKSHIYKEISPNSILVSGGQTTVANLFYNMARRTVGLVGVW
                     DVVAFDEVAGITFKEKDGVQIMKDYMASGSFSRGRDAINAYASMVFVGNINQSVDTLV
                     KTSHLLAPFPDDMIDSAFFDRFHAYVPGWEIPKMRPEFFTNQYGMIVDYLAEWMREMR
                     KRTFGDAISKYFKLGRDLNQRDTIAVKHTVSGLLKLLYPNEEYNKDAVRRCLEYALEA
                     RRRVKEQLKKIGGMEFFDVHFSYIDLETLQEKFISVPEQGGGTLIPDGPMSPGALHTV
                     ATGSGGHLGLYRLETQVTAGNGSLKTSGLGSNSGAKEAIKVGFDFFRANASRVSASIK
                     AGDHDYHLHIVELHNTGPTNALTLSAFIALCSAVFGKPIQSQMVVLGSMSLGGNVIPV
                     ENLAESLQVAFDSGAKRILLPMASVRDIPTIPGELFAKFQTSFYSDPVDAVFKALGVE
                     "
     gene            38636..39100
                     /locus_tag="PK614_00185"
     CDS             38636..39100
                     /locus_tag="PK614_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80664.1"
                     /translation="MNLNEIIDSLRTEDLVSQFKRALKDAGVEIAEDPVAKYFAAETV
                     DATLLVLAHESTLEKGWWGIHSDIVERAKNSERVKMNHVGWGAVLLHKSFQRGYWIRG
                     DDIFRMKTLCRVTLGKEGQYHFNGDSLENSPEFANQFFSVKTFLKLSGLQKS"
     gene            39156..39620
                     /locus_tag="PK614_00190"
     CDS             39156..39620
                     /locus_tag="PK614_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80665.1"
                     /translation="MAGIRSFPSPSILGRAHNIYIDDLWKSDGWGCTQWRGYSAQMLF
                     WYGVAGAPYAGGSDLADRYLASIIGKRKISGVPLAKLKLWKSVNRLVNDIRRETGMEL
                     VPWLAQTRIDSAWERLQQVYLIGPKIASWFLRDISFLMDYRIGGGPSRFSYG"
     gene            40198..41016
                     /locus_tag="PK614_00195"
     CDS             40198..41016
                     /locus_tag="PK614_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80666.1"
                     /translation="MKHSLPDLVKAQLRQAVGIPIDANGYVSRVIDNLLPGVRLDHFE
                     SDLKRGGGHELNKKSLALHSSAALAINTFAPFKDFPRSLSILGKQGFRPPVFEKQLPT
                     GLSGTAPNIDVYLQSDDEVIAIESKFLEHWTPKRAKYSGSYCKDSLPLAEDCWWQVLE
                     ESKAGGLKHLDVAQLVKHYFGLIQLMKQVEGRKHKEVTLLYLYWEPANACDIQDCLGH
                     RRNIGELASKVSGSRIKFKSLSYPELWREWEGMPGIAAHVRNLKARYALTLKLA"
     gene            41115..42158
                     /locus_tag="PK614_00200"
     CDS             41115..42158
                     /locus_tag="PK614_00200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRB80667.1"
                     /translation="MMGRSYFVRAMSQTEKEFNVFFENSVVAVGWSSVDMSESTNIDK
                     IVDAVDCAYYRGKPTVPQVVGRKLNEVRRFTGLSAGDQILVPYYSSVRLAVVENGQVY
                     NSSPELDTDHLDLANQRQVRYLKIGKDFVEIPRTNLSHGLQSRLRMRGTTIGSLTGFE
                     TEIGEIFNDPKTTWKSRFAMSDRKNAQEWQSNLLASIQNGRTNLKAGGRGLEELVRDM
                     LTLEGYQAKTLSTRRFPGIADADVEAVRADLFAQTKLLVQVKHHYGQSDAWGAKQLDE
                     IFNDPEAFVDYQPVLVTTADSSEQLTEYCESKQIRLITGRELVERIYDQLERLSSDWR
                     ETLGIGKTGQLLF"
     gene            complement(42293..44344)
                     /locus_tag="PK614_00205"
     CDS             complement(42293..44344)
                     /locus_tag="PK614_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80668.1"
                     /translation="MPNIGLMIIHGIGHQQEGTIARTIASELTRLDNSITSLVQPTEN
                     SPVVSIQLSEQTVLIAEGHWADLSHVDNLPILHLKSDTFINVLQSLKHGWNYLVFHQR
                     VPSTSNPIAFFSYVGMSIVAGIWLVTQLLLISGRLWVSLPPQLKITVIQLENSKVLLI
                     CFWIFIGANILFVMRQWVKTFVEPTTAHWATAGIFFIPALYAGMFRAALASFGFIIGV
                     PATVALLILALVTNVVGVVFLYPLQQLSSALTGWATPKFLCWIHRFAHVTVVMPVQSI
                     VQAAKSFGNLIVLVSSDHKLCDRLVAAIWINIIFMVFALYFLLAGVILSPVLGPLGDI
                     IQKWRDDLDKTIPLDLIISSLESLLIGLALIVLLAQWKWVNTLFDLILDVSNYHLASI
                     SDRKKLWDRVEAGYQALQKVGCTQIHILAHSLGSVIVYDWLHNRQTLPEATITSLYTI
                     GSPLDKFWYIDYASNRNPPPESNDRGPRIMSWTNFWSILDIVSGKLHHVRPISGTIDN
                     VHLWGLGYPLACHVDYWTNATVLKSIYNTILHGPTSHTGDLAPQSSTVPPQVKFRLFS
                     SWRFVMAVVTAWLTHGLLLTWLQYNYARSHNKLASTIGIGSYFEERIAEVFSAIISHG
                     IFGLIFGTIAWAIFRRYRTGGGSIPIDHDYRRWITFWGMITFMLLALLRASQTEFTLM
                     R"
     gene            complement(45341..46111)
                     /locus_tag="PK614_00210"
     CDS             complement(45341..46111)
                     /locus_tag="PK614_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80669.1"
                     /translation="MRQSLMSDAAQFVNSVDLPTPKRGAVESAGVFDFDSARNQATVV
                     GSDIVSFVKGVTPERRNDIVRSALLAQLVAKKRIANPNDIYGWYDEYFNVLTNIGWVI
                     QERSFADYNESSSDFTAHEAVLKVAAALLGPAAPALAVLQATLGALKDMSADSPWIRL
                     FSRESQSASSGRFQIMLVEQGDSDQFLVSLLAFGVEATSNLTQVLFFKFRSNEVRLRH
                     NSAKVSIDSEVLESVREAIKNKISLFVQDYVLALPDLS"
     gene            46480..47544
                     /locus_tag="PK614_00215"
     CDS             46480..47544
                     /locus_tag="PK614_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB80670.1"
                     /translation="MPQDLQEVKRLTGSEIEAIVEAIVDGYNRAGLEQALLFDWGEIL
                     ENRVNVYTGLFNIATELIRWTELKGRTLDLVRLLYERNSGNPRLRQVAERYRLAASNA
                     PRPTSLESLISTRSRFLDYDRFTSRFATFGRRVCKITTQHRTGTGTLIGPDLVLTNFH
                     VIEEVYYAPSLATAVRCHFSGIGGADDENDLQCGLAPIPNWIVQHSLCSSSDITGTGT
                     AGMDELDYALLRLEKNIGEETSHEGHRRGWFKLGESDPIMGRGDFIVIPQHPNGSSLQ
                     IAWGQLLEPNSTGSRFRHDATTDGGSSGALCFNTELEPFGLHHATQPVMVGTNPHYNQ
                     CIPLRLVAADLNKHQDKTHG"
     gene            47537..48583
                     /locus_tag="PK614_00220"
     CDS             47537..48583
                     /locus_tag="PK614_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80671.1"
                     /translation="MADPESGQVDPKYWEGIISQLVQCSPSDLLDKILLKWRSTKLES
                     LVGITGRPPKDWIYELFTQANKQSWLSLLLIRVQSWTQHPGLRTEIGIALGDFGSARP
                     GWYDVPVAIGRAVIDRSQFRSTLFEFLERDSANIRLLVVRGDNPGKSHCRWLAQYVAN
                     QIGMTTLYIDLLQDASRLRSDLANRLSLHYAEFMEGFTTSARDSKEFVGWLAGKLNNA
                     PLLNSRYLLIFDHIAKPGVPVEAREVVIELARKAVNQELTNIWVILIDTEGLGEIEHP
                     GASSEELVPQVTRHEVNNFLEFAWRFRGGIGHTPRNVPVALTEMLDGSIVALSKENLK
                     KAEREIAQWMRNIT"
     gene            48549..51401
                     /locus_tag="PK614_00225"
     CDS             48549..51401
                     /locus_tag="PK614_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB80672.1"
                     /translation="MKSLNGCVILLSLNICTAMKNKGNFKKLSIELSATADLLADWKE
                     VSPNQEISWAYRLAAAVMDTFNPGTIQPYDENGSILSQSYPNPENDADLVIPQIPERW
                     RLHCDLRREALEKLATRQNMKDALALNPDIADTSLQRMLTSAINDTIPDDLRALTRDD
                     LSALVTVASWLKSIIKIPDLDSVARVFALKDLLSPFEHLTSDGFVGREMEITLLHSYL
                     TSDQDGTEPLLLYGVGGVGKSSLLAQFILQAVRDPTNTPFLVHLDMDSPTVSPQHLHT
                     FVIEITRQLLAQDPGLSAHGRANLRSVRDLAKRLPSDLERRYSESTGSLLLEYLLNSA
                     SALLTNALESSKERKVLIVLDTFEEAQIFGESVAYSILMFMKLLQSRVHNVRIVIAGR
                     GTPPEGLRKRVGLERHPVQINSLDPRSAGILTYHLLEKSHLQHDEAVIQEIVSVVGRN
                     PLSLKLAVRAISLQGFSAFLDTDSRSQIVEKLRQERVQGYLFGRILNYLKDERLKKLV
                     YPGILLRRVTPQLIWEVLKEPCEIEVQSIDDSTELFRSFEKVMGVVEPDGNEGLRFRL
                     ELRREILGDVLRVVDPKIAEAIDARAVKFYESDTTPFGRAEELYHRLRLNQSNDILEA
                     RWSQNAWSHLKQSITELPAQSQLWLAQKANVSLDVAVRRKASDDEWEDEAARMAESLL
                     KLGRADETLEVLRERPARPRSSKISLLEAQALRLLGREDEASEILILGLKEASIPQKV
                     EILLLLALIDENRDQLHRALQTASQAFRISSKTSDILLKLRTLVTRLRLFRKLNMSSE
                     HEKLRAKMVKSVSEKVLKLLRDHPALFQEVAAELGASSQKILKAAIDRLGMLITSKSQ
                     REALIRALDQLTTSVSVSNPSRKHDELGISDVDEILRSGGAGLGDKVVGILGGKPVDH
                     TVLESLTEYFRGSVDVSLSQNWSEGLFGKGKRGENA"
     gene            complement(51851..52936)
                     /locus_tag="PK614_00230"
     CDS             complement(51851..52936)
                     /locus_tag="PK614_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB80673.1"
                     /translation="MGLTKRKNVWWMSFMFHGQQVRRSTGTTKKQLAEAIYAKVKVQI
                     AEGQFFERREEQERTFKEMMERYVTERATLKAIKSRLRDQAALGHLLPVLGEKVLAHI
                     TPKVMAQYKAQRRTEQAAPATINKELQLVRHAFNLAMREWEWCRENPMHRVSMEQVRN
                     EVDRWLTAEEEERLMAASSPWLREIMVFALNTGMRQGEILKLQWQEVDFSRGTLIVMQ
                     SKNGTRRTIPLNTRVYELLAAKQAATGLSRGPVFKTPLGNLLQVRFLVREFCEARNRA
                     GIPDFRFHDMRHTFATRLVQRGVDLYKVQRLLGHKTNLMTQRYAHHSPESLREGVKVL
                     DECQPERFSTNLAQGALSEDFRGLNVS"
     gene            complement(52917..53237)
                     /locus_tag="PK614_00235"
     CDS             complement(52917..53237)
                     /locus_tag="PK614_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB80674.1"
                     /translation="MLTVQEVSDWLNIKPGTLYSWAAKGKIPCRRMYGLVRFEREAIQ
                     TWLDTCNNIPQKPLPFPIRHNSCDIDHVIEAAKREVYTPRHGETRPKSGLIQKEDADG
                     ADKT"
     gene            complement(53280..54290)
                     /locus_tag="PK614_00240"
     CDS             complement(53280..54290)
                     /locus_tag="PK614_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB80675.1"
                     /translation="MDSPFTLSIDWLAFTVPASNPEETMRTLGGEWNKAKAGFRGYPL
                     SWIRVDGLRGVGKLGTNAPRRPNEIHVDLSGGIVSAYTLQEVRSLLLWVIEKQGHLTR
                     IDCALDDRAGTVPLATIAQALEVGQCVTRAKEVRRFVSNRIHDSYATTGETIYVGSPQ
                     SETLLRIYDKRLELQSNERENWQDYGVRWELEFKKTRAKICGNALAYFEESLWREFIV
                     GLLRSHVDFRQTTREDDDEYRCRAPLVEWYAVLTEGFQRARLVLEKQEQTLTGVKNWV
                     SGSLTPMLAVLCATPGGEAWLQDEIVRGVSRWKDRHRNLLKKKPKRSVSTPAGGNAGS
                     PL"
     gene            complement(54448..54861)
                     /locus_tag="PK614_00245"
     CDS             complement(54448..54861)
                     /locus_tag="PK614_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80676.1"
                     /translation="MNTRDLHAIWNAPDNSRLTAKQYTVRLPIRVAAQLAALNEMYPR
                     KTMTDLIGDLLAAGLDQLAEDLPTKNVGEKPLGFREDGEAEWEQIGPGVTFAQLSQKY
                     LKEFESEEATKGNTEKPAKKIQKPAGTREDRKAKR"
     gene            55041..55496
                     /locus_tag="PK614_00250"
     CDS             55041..55496
                     /locus_tag="PK614_00250"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HRB80677.1"
                     /translation="MAGDDPHHFDFDLDRGIRSQVVEKLESSPLFPLKKGIGPQASGI
                     YALYFRSTLVYIGKASKGTTKSKRTLRGRLGEHVGKISGRRNISLADMECRYLTFDSE
                     WWVFAAEFALMTHYRPEWNESGFGSKAPGVGRPGTHRVSRWNEQFPVRG"
     gene            complement(55493..56251)
                     /locus_tag="PK614_00255"
     CDS             complement(55493..56251)
                     /locus_tag="PK614_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80678.1"
                     /translation="MPLETTVLDRFTDIVLRYNPKEKSAGQSKIREAENVVSTYAPIS
                     SIWEGSDGDLLEAIFTFYATIPPEPILDSTYNAGRFWKGSKRDVVSMDIDPRYKPMIV
                     GDNRKMKGVPSSKFGVVVFDPPHVGPQGRDKSRKRFDVDFGATMECGAEQDWNLSYLY
                     PAFLTQAKRVLKPNGLLLAKITDMVNNHKSKWPHCDFMRMAEEAGFTVCDLIIKVRKG
                     PMVSTKWKTAHHARKRHCFWIICRNSDDCERGCP"
     gene            complement(56318..56827)
                     /locus_tag="PK614_00260"
     CDS             complement(56318..56827)
                     /locus_tag="PK614_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRB80679.1"
                     /translation="MNKVTLSALIKRFPDADSGRKYLEKRLWPNDITCPACKGSDRIT
                     SRKGGYYRCNACKEDFTVRTGTIFERSHVPLHKWIYAMYLLVTVRKPISSLQLSKEIG
                     ITQKSAWFVLQRLREASDSDQTMLSGLVEVEETYTGDKEGNRHKSRARHIPIGKSAAI
                     ACVSSPGAR"
     gene            57511..57876
                     /locus_tag="PK614_00265"
     CDS             57511..57876
                     /locus_tag="PK614_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80680.1"
                     /translation="MTRRLSKQWQVRGWTCAVLLAATFTTGCMHHPGGIAPSTKPLAP
                     GGYTELGKVRGQDCVYHLLGLIPVTGGNEMRNAVEDALRTKPLADAMVEVTVDGYFQY
                     FILFSRACTQVYGTAVETK"
     gene            57980..59068
                     /gene="ada"
                     /locus_tag="PK614_00270"
     CDS             57980..59068
                     /gene="ada"
                     /locus_tag="PK614_00270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HRB80681.1"
                     /translation="MTSSASTTRRINRNIRQDLRWAAILTRDPAADGTFYYSVRTTGV
                     YCRPSCAARQPLRENVRFHATCADAEQAGFRPCKRCRPDQPSSVDRQAGMVVAICRYI
                     EQAEHLPSLDELAQRAKMSPSHFHRIFTHVTGLTPRGYAVAHRATRLRFELGRDRPVT
                     EALYQAGFNSSGRFYEASNALLGMTPSRYRAGGAQTEIRFAVGQCSLGAILVAQSERG
                     VCAILLGDDPDALTRDLQDRFPLANLTGGDAAFERLVSTVVGFVEAPGRGLKLPLDIR
                     GTAFQQLVWQALQRIPVGSTATYTEIATRIGSPKSVRAVGRACGANPLAVAIPCHRVV
                     RNDGGLSGYRWGVERKRMLLERESRVAP"
     gene            59391..59831
                     /locus_tag="PK614_00275"
     CDS             59391..59831
                     /locus_tag="PK614_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB80682.1"
                     /translation="MGMAWPFGLVADEKVCLGRVLVVDDEDSIRSLLRMTLAQAGYDV
                     EEAEDGGRAVEVLNSGDNPLMVDVIICDIRMPRINGVEAIAYFRAQYPSLPVIVLTGY
                     HDEQLASSLRKQGVVECLEKPAEREQILTSVAQAIAGRRISFYP"
     gene            59974..60642
                     /locus_tag="PK614_00280"
     CDS             59974..60642
                     /locus_tag="PK614_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80683.1"
                     /translation="MCHFIVRSSPHIPLRHLVAASLVGLLFIGLAPSPLNAARGGGKT
                     EVLPVQKAAPAAVIIEGLGNEAELRRQMRAKNGVAELGASTAQVMFSQAPTGTFGFIV
                     PKLLGLALVTQSPDLAVARVAPAENAYEIHKLADGSGMLVGFMGAELMTQVAPSQRPK
                     NVRIALYSNPSEKAPHIVAVPLVKLVADRMPIRLDAKNPNGPVLLDMDLQGSSTRAST
                     QGGQ"
     gene            complement(60717..61055)
                     /locus_tag="PK614_00285"
     CDS             complement(60717..61055)
                     /locus_tag="PK614_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80684.1"
                     /translation="MKRLVLSACALTIGATLALNSWCAAENTTTINILSPRNGEKVSE
                     TFDIKYEILNAPAGNHAHVYLDGAYQKGFKGTFTSVAKGEHTITVKIADHDHKDAGTA
                     TDSITVKVGE"
     gene            complement(61066..61800)
                     /locus_tag="PK614_00290"
     CDS             complement(61066..61800)
                     /locus_tag="PK614_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRB80685.1"
                     /translation="MQNTSGLIRSIQVGLPSSEHSDCADDPDSTPWTTGMFKRSTPGQ
                     IRLGRHNLAGDGQADLVHHGGVDKAVCAYPSEHWLYWRGILPPHRLTGGAFGENFTLE
                     GLTEADVCIGDVFSAGTAVVQISQPRQPCWKLARRWQIKDLAVQMERTGFTGWYFRVI
                     QEGTVEPGAPLQLMERPCPEWTVATANRIMHHERNNLAAAEHLSLCPLLSASWQQTLR
                     QRCHNQAGPDPQHRRFGTTHSSSSSF"
     gene            61929..62825
                     /gene="cynR"
                     /locus_tag="PK614_00295"
     CDS             61929..62825
                     /gene="cynR"
                     /locus_tag="PK614_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HRB80686.1"
                     /translation="MEFRHLRYFLAVADALHFTKAAERLPVSQPALSAQIKQLEQEVG
                     VPLFDRVGRSVQLTRAGSIFREHARRALREMELAQVAIAEEEGLQRGTLTVGVVQTVN
                     AYLIPEIVGRFSTLHPLVSLKLDELSGPDIEAGVKSGSLEVAIGFVPVVSDRIESQPL
                     FEEDFVLIASPRHRFAGRRHLSLSALAQESLILLPNMFCTRRLLDGCFEQAGVQPKVI
                     VEMNSIEGILATVRRSNLATVLPRLSLGLARNQTLRAITLKNPTPRRGIGLLWKKGGY
                     RSGAAKALTDQVRAVVGEHWRS"
     gene            63189..64427
                     /locus_tag="PK614_00300"
     CDS             63189..64427
                     /locus_tag="PK614_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HRB80687.1"
                     /translation="MAESVRFATLVKRWMIGLCAACLLAVGVYVLMTRSGEAQPRQAE
                     KRPAMGARSTPVVAVAAKTSEINIYLNGLGSVIPMNTVTVKSRVDGQLMRVLFKEGQL
                     VHSGELLAQIDPRPFEVQLIQAEGQMARDQAQMKNAQLDLERYRDLYKQNFIPKQQLD
                     TQEALVRQYEGIVKADQGQIDNAKLQLTYSSITAPINGRVGLRLVDAGNIVHANDPNG
                     LLVITQLQPITVVFALAEDHLPSVFERLKAGKQLVVEAFDREQKRKLATGTLLTVDNQ
                     IDPTTGTVRLKAVFPNDDSSLFPNQFVNARLLLDIKQGVTVVPSAAVQRGPKGTFVYV
                     VNEAEQTVSVRAVTVGVAQGEEAAIDTGLTSGEVVVVDGTEKLREGSKVELRPAAGAV
                     PASQEAPAPGGEGRTRGKRT"
     gene            64424..>65404
                     /locus_tag="PK614_00305"
     CDS             64424..>65404
                     /locus_tag="PK614_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB80688.1"
                     /translation="MNPSRLFIVRPVATVLTMIAILLAGALAYRQLPVSALPQVDYPT
                     IQVMTFYPGAGPDVMTSSITAPLERQFGQMPGLNQMTSTSSGGSSVVTLQFSLDIDLD
                     VAEQEVQAAMNAAATFLPRDLPAPPVYSKVNPADAPILTLALTSATLPLPQVRDFAET
                     RFAQKISQLSGVGLVSISGGQRPAVRIQANPRALAAYGLTLEDLRTVVAAANVNQAKG
                     GFDGPRRASIINATDQLFAGKDYKALIVAYRNGAPVRLSEVAEVIDDVENTKQAAWMN
                     ETPAVIVNIQRQPGTNVIEVVDRVKRLLPQLQGTLPSSVQVSVLTDRTTSI"
BASE COUNT        14793 a        17210 c        18746 g        14655 t
ORIGIN      
        1 tgggagcgtc cgcaggcttg atcggcgggc cgacgggtat cgccatcggg gcggcgtcag
       61 gtggcgcggt cgggggggtg ttgggtttcc ttgccgggac ctacaaacca ctgcaaccgc
      121 agccggacta tgcggccttc gttgaacgtt gtctcaagga aaaaggatat gagacgaccg
      181 gctggcaata acgatacatc tgatgttctg tgcgtgcaca ggccggatcg atcaaaaggt
      241 gaaacagccg gagtagtcgg ccatcaggca ttgcacacgg atcgagatcc gctccagtcg
      301 atcacggtcc tgcggcgtcg gcgctgaacg atcggcatcc cgcagccatt ccgcatcctg
      361 cctcaattta cgcaacgacc atcgcgacgt gcctggaatc cgcgtctcgt cttgaaccgc
      421 ccggttgacg atctctcgga acgacaccac ttcatcggcc cactccacac gatcggcctg
      481 aaatccgtag ggcgcctcat cctgaccgat cagggcaatc aagtgtcgca gccattcgac
      541 ggtggcggcc atgcggtcgt aatgcagggt gctggtgacg tctccggccg tgtggtaccg
      601 cggggtgcgt ccggcggaga gaaacagaaa cggcagtcca tggttgcgga agacgtcgta
      661 gtcgctgaag gctttgtggc cgatttcggg aatctcttcg accaggtgca tccccacagg
      721 gaagaccgtc agcgatggtg ccgaggcttg tttgacgcgc tggtacaacg ccggactttt
      781 ttccgcgccg gcggcaaaaa tcgcatcctt gatagggggc cactgtacgc cacccatgag
      841 gtccatgatc acgacggcct ggagattctc ggcacggccg atttccggcg gcaggtggtg
      901 aaacaagaac tgcgacccca tctcagcctt accgaaatac ggggattcct cgctgttgaa
      961 ggccacaaac agaatgccgt gctgggggag gcctgccatg tgtcgtgcgg tttcaatgag
     1021 gatggcgatc gaggaagcat tatcgtccgc acccaggaat gggtaaccaa gatgatcgta
     1081 gtgggcgccg atcaccatcc attgcgcggg ggcggatccg gcggtcggcg ggatgaagcc
     1141 gaggagattg tcgccgatcg tgggcgaaat ggcttgccgg tagccgccga gtgacggcag
     1201 gggttcgagt ccgacggctc gaaactggtc ctcgatatag cgggcagcct ggcgattccc
     1261 gggtgagccc ggctgccgtc ctgacagctc ggggcttgcg agaaacgaca cgtggtgctg
     1321 caagcgttgg ccggaggtcg aggtaatcgg caagctcctc gggtcgatcg cctccatcgt
     1381 acggaggcat ccggtagtag ccaggccgag caggagagac agggcggaga gcatgagggc
     1441 aacggcagga ttccggccgg gaggcataga ctcgatcacg gttgcggggc ggattccggg
     1501 gtggtcggtg gagtggtttc tttcggcgcc atcggcagtg ccttgtgccg gagcgcgcgg
     1561 cctttctccg gagtcggatc ggtgatgagc ccgtaacact ggcggccttc gtggtcttcc
     1621 ggcagatatt cggtgatcgg catgccgtct tcacggacga agagcaggca gtggcagtat
     1681 ttgtacatct gcatttcgtc acaggcgcac atccagcgcc gcagcttggc ttcggcctgc
     1741 ttgtcggggt agaagttgca ggggcataac ggcttcccca actcgtcgac gtgggcggcg
     1801 agaccgagga ccactgcgtt ggtgacggct tcattcgggt gcatggcggt cccgctcttc
     1861 tcggcgaagc cctttacata cttccacatc ttgtcgaggc tttctttgct cggttcagcc
     1921 atcctgtccc ccgtgttacc actgcctggt gaatcgttgc ctgtgcgcca tcggtccctg
     1981 cccgccgtga agacacagtt tacaagcgaa attgtgtcct ttacaatcca caacttcccc
     2041 caaaacggat tctggcacta tggcgtctgg gcgctcctct gaacaactgt gtgacctgct
     2101 ggccgaacaa ctcgggcctg acgcggctgc tgccgtggtg aaggctattg ccgactcggc
     2161 tgccaggccg gatgcccttg atggtgtcct ggtgttgttg gacgagctca cggaagaggc
     2221 gccgaaagcg gccaaggctg cgatcgatgc gttggccgat atgcagcgtc gcggcctgct
     2281 ggcggatgtg ctgccatggg tcgatctcgg ggtggccatc gcggcagatt ccggtgcgtt
     2341 ggccctgaga ttttttaagg agagtccgct gctgctcggt ctcctcgagc cctccagcag
     2401 agcgattgtg ctggctgccg ggttagaact cgcagaccgt caggccaatg tggcggtgga
     2461 atttatccgg attgctccgg aagcggtcgc tgtcttgtct ccggcggatt ggcccgtgtg
     2521 gatggatctg gcctgtgaac tcacggaaac cgactttgcc ctggcggtgg aatatatccg
     2581 gcagattccc tcgattgcac gcgtgttgtc actcgacgcc gttcgtgcct gggtgcggtt
     2641 cggcatgaag ctcatcgtcg agaatagtct cggcaagccg gactacctgg gagtcttgga
     2701 attcttcaga acgagtccgg ccatattgga agatatcccc gggccgtcac atcgcaatca
     2761 ggtgatcggc atcggagcgc agatggccga gcgttcgccg gcatcgggta ttgcggtatt
     2821 ggcggaggcg cctgcgatgc tccgccgtct tccttcggag gaatggcgga ctcaggtgct
     2881 ccgttacggg ctgctggtcg ccgagcgtga tgctgagacg gcgctggcct atcttcgacg
     2941 ctgccctgaa ttgatcgcct tgatcggatc cggtgaggga gcgcaggaga agttccagtc
     3001 ctggtttgct tccggcatgg aagtgctgga gtacagcctg gagggggggc gggcctactt
     3061 cgctatggaa acggagaagg ccttgggcgc cgtgtctcag gccatgagcg gcgtgccgct
     3121 gcggcagatc gcccggcgcg tcaaattgtt cgcgcaggcg ctctgtggtc gcgatgtgcg
     3181 cattcacgat ctccccgact ctctcgaatc gacccagccg atggctcgtg ccacggtgag
     3241 tgaagacggg cagagcatcg gcctgccgtc cctcgtcagg cggtatccga cctacgatga
     3301 aaacgtgcgt ctctatatgg tcatggcggc gcatgaggcc ggacatctgg agttcggcac
     3361 atttgacttg cccatcggca gactacagga cctggttgag gaccttgcgc agcgttatgg
     3421 gcgagctgtg ccgaatgaag cccggacttt gggcgatgtg tttgcgcggt atccacagcc
     3481 cggcctgatt cgcgacctct gggctcttgt ggaggacgcc agagtggagt acctgttgcg
     3541 cgccgagtat cccggcttga gccgtgacct ggcatccgtg gccaaagacg cggtgaacgt
     3601 tcgaagtctt gcgcatggca tgacggtgcg cgaattagtg gtcgatcagc tcctgctgtt
     3661 ctccaccacg gatgccggaa cggttatagt tccggacgcc gtcaaaagcg aagtcgatca
     3721 gctctggtcg ctttgccgga cgatctacca tcctcgagcc accgccgagg aagcggtgcg
     3781 cctggcggac cgcctctatg tgcgcatgga tgagttgttg gccattcgcc gggagtccat
     3841 tcccagtcag gacgagacgg aggtcttgga acaatcgatc ccggcaccca agtcgtcgga
     3901 ggatctctcc gatacgtaca ggccgatgga caattgggac tatcgcggcg cgatggatcc
     3961 caatctggtg cgcgaccgat cggaatcgtc tcccgaacag agtgcggcat ccggtcaggg
     4021 cgatagcgaa ggcggggcgg gtttggccgg tgatgccgga ggttcgtcag gcgcgggcac
     4081 cgcgcgttcg cagcagatgt cgcaagatgt gcttgtcccc ggccgccgaa agccctcgct
     4141 cgtggaggag gttctggcgg ttgaaggcga gggggctcgt cctgaggatg aaacagccgg
     4201 cgcggtcaag gcggttcgtt accgtgagtg ggatgctgcc attcaggact acagaatgaa
     4261 ttggtgccgg gtggttgagc gcgaggcgat tgaagggaca tcggactttg tggaggagac
     4321 cctcgcggct caacggggaa cggtcacgct gttgcgtcgc tatttcgaaa gtctgcgccc
     4381 acccgggctc cggcgcattc ccggtcagtt agatggagag gacctggaca tggatgccgt
     4441 gatcgggcgg atggctgatc tgtcggcggg catcgagcca tcggaacgga tctatgtgcg
     4501 gcgtgaaaag cgggaacgtg aggtcgccgc cgcatttttg gtggatctca gcggatcgac
     4561 cagtcgtcag gtcgagcagg ggcggcggat tatcgacatt gaaaagcagg ggctcgtgct
     4621 cctgtgcgaa gcgctgacgg cgatcggcga tcaattcgcc gtgtacggct attcagggca
     4681 gggtcggaac caggtggatt ttgtagtcgt gaaggggttc gacgaactcg tgaccgggcg
     4741 tgccggggcc aagttggggg ggattgtgcc cctgcagcag aatcgcgacg gcgcggccat
     4801 ccggcatgcg acgagcaagt tactcgcccg tcaggcgaag acgcggattc tggtggtcat
     4861 cagcgacggc cgcccattgg acgacggcta caaggatgac tattccctgg aagatacacg
     4921 catggcgcta cgggaagccc gtgccagcgg tattgaaccg gtctgcatta cgatcgacaa
     4981 gcagacggat ccctatctgc gtcgaatgta cggggatgtg cgttatgtgg tgattgatcg
     5041 ggtggagggg ttgccggaga agctcccgag gatttaccat cggctgacgg cctgaatggt
     5101 cgtgcgcgag gccttttcag cactggagca tacgaacgat gattgagcag ccgacacgac
     5161 cgactgtgcc accgtggctt tacaagttat ttaccggcca tcaatatccc tatgtgcgac
     5221 ggcaggccaa atttgcgaat cgtgatacca agccgggaga ggaacggccg gaaccgacgc
     5281 gggaggagat cgacgccaag ttttgggaga tttatccgcg ttgtaccgcg aagatattgc
     5341 aggaagtcaa agccggcatg atcgtggtat tccacgaact gggcgaatat cctgccggcg
     5401 ggtaccaggg attggttgat gcgcccgaag agtttctcgc ggcacactac gggaagaaga
     5461 aaatcaaagt gaatttttat gacggcgata attttgtctg cacgatcaat ttcaaggtcg
     5521 gcggctggac gggtcacgaa cacgcgtgaa gggtaaacgg tgagtgtaag ggaactcttg
     5581 cacttgacgc ttcacgtttc acgcttcacg aggtgggatg atgggacgac caaagattac
     5641 ggtagtgggc gcggggaatg tcggcggaac ggtcgcgcag cggctggcgg agaaaaatgc
     5701 atacgatgtc gtactggtcg acattgtgcc gggcattccg cagggcaagg ccttggatat
     5761 tacccaggcc gggccggtgt gcgggtacag cacccgggtg gtcggtacga atgggtatga
     5821 cgaaaccgcc gggtcctcca ttgccgtcat cacgtcgggg atgcccagga agccaggcat
     5881 gagtcgggac gaactcctgg ccacgaacgc caaaatcgtg aaaacggtgg tgcgggaact
     5941 ggtatcgcgt tcgccgaaca tcatccttat tttggtgacg aatccgttgg atgccatggt
     6001 gcatgtggcg cgacaggtca gcggactgcc gaaatcacga gtcctcggga tggcgggtgt
     6061 cctggatacg gcgcgcttgc ggtcgttcgt agccgaagaa ttgaatgtac ctggcacgga
     6121 ggtgcaggcg atggtgttgg gggggcacgg ggatacgatg gtgccgctgg ttcggcaaac
     6181 gacggtggcc ggaaagccga ttacggatcg attgtctccg gagcggctgg cagccttgat
     6241 taagcgcacg caggacggcg gggccgaaat cgtggggctc ctcaagaccg gcagcgcgtt
     6301 ttatgcgccg tccgcctccg cggtggatat ggtcgaggcg atcatgaacg accagaagcg
     6361 cgtggtgccc tgcgccatgt tctgcgaggg ggaatacggg ctgaaggatg tcatcgtcgg
     6421 tgtgcccgtg accttggggc gcagcggagg agaatccatc gttgaatatg agctgaccgc
     6481 tgaggaacgc accgcactgc aggcgtctgc ggacgcggtg cgggacttat gtgcggtggt
     6541 cgaccgccta ctgactgcct agccggctga ctagggtgtg tggaggcggg ttgcttgaca
     6601 aggctcagcg gcctgcagta gagaacgagg ctggcactaa accgttacag ttggggggcg
     6661 tatgaaattt ttggaagatc catatcagac attgggcgca ggatttgcgc tcgctgtcgg
     6721 attgatagtc gtctatctgg gtatggctgg agtgggggcg ggcgatgccg actggttcgg
     6781 cttgatcgtg cgctggatcc atttcctggc cggcatcacc tggatcgggc tgttgtactt
     6841 ctttaacctc atcaatgccg gctttctcaa gagcctggat gggccgacga agaacatcgt
     6901 cattcccaag ctgatgcctg ccgcgctgaa ttggttccgc catggcgcca cggtcaccgt
     6961 cctcgcgggg atcgtgctct acggctacct ctattcaaaa ggtggaacgg gggcgcttgc
     7021 gctggggatc ggcggtttgc tcggtatcat catgatgggg aatgtgcacg ggattatttg
     7081 gcccaatcag aagaaggtca ttgcggcggt ggctgccgct gcgcagggta cgcccgctcc
     7141 gccggagatg gctcagtggg gaaaaacggc cttgattgca tcccgcatca acttcttgct
     7201 gtctattccc atgttgttct tcatgggagc ggggagccat ttgaagtaag ccgtcgggtg
     7261 agcagaatca cggggattgg tcatccgact cgggtggcca gtcccctttt tcttgggccg
     7321 atcgatgccg ggtaccgtaa tcgaatgtcg gcgagtcttt ggactgattc gctgtaggcc
     7381 tatcaggcct aactgattga gattcaggcc taatcaggcg aacttggctt gacggccccc
     7441 aaagagcagc cgtatagtac attcctgcta acctgggggt gctacccccg aaagggaata
     7501 acgtgaccct acctcaacca agggtattgc agaagcttga aggggctccc tgggaaatcg
     7561 acccctacct caagaccggc gggtatgaag cttggcggaa gtgtctgaaa gagctgaccc
     7621 ccaacgacat cgtcggcgaa attaagaaag ccggattgcg cgggcgtggc ggcgccggct
     7681 ttccgaccgg tacgaagtgg gacaaggtcc tcaaccatcg agttcaggag cgttactttg
     7741 tctgcaatgc cggggaacat gagccgggca ccttcaaaga tcgtcatctc ctgaagcata
     7801 tcccgcatca actgatcgag ggctgtctca ttgcctctcg aaccgtgaat gccaaagcct
     7861 cctatattta cgtcaaccat gagtatgcgg aagaagaggc gaatctcagg aaagccatcg
     7921 cccaagcccg ggaacgtggc ctattgggga agaatatcct cggaactggc gtggacctcg
     7981 accttgaagt ctactctggt catggcagct acgtagcggg tgaagagacg gccatgttgg
     8041 agtccatgca gggacgtccg gccatgccgc gtcagaagcc gccgttttac cccaccgatt
     8101 tcggactgta tgggaagccg accctggtga acaacgttga aacgttgtgc aacattcccc
     8161 tgattcttaa aaacggcgcc gcatggttca ctcaggtggg gacggagaag tgtcccggga
     8221 ccatgctctt ctctcttagc ggagcggtga atcggcccgg agtgtatgaa atgccgatgg
     8281 gcgtgacgat cagggacctc atcgagacgt gcggcggggg tgtcccgaac gggcgcaaga
     8341 tcaaggcagt attcccaggc gggcctgcgt tctcaatggt cacggcggat cagctggatc
     8401 tgccgatgga tttcgattcg ctgaagaagg ccgggaccgg actcggttcc gccgggacga
     8461 ttgtgatcga tgatgccacg tgcatggtgg cggcgacact caagtactcg aattttttca
     8521 agggtgaaag ttgcggccaa tgccctccgt gccggatggg tacgatcaat ctggcgacat
     8581 tgatggacaa gatcgagcgc ggagagggga gccagaagga cctcgattcg ttgctgcaac
     8641 tgtgtggatt tgtgaagggc accgggtatt gcacccttat caccggtgcc gcagtcctgg
     8701 tgcagagcag tctgcgattg ttccagcatg aattcgaaga gcatattcgt ttgcaacgct
     8761 gtccctttca gccggcccgc actggggccg gtgcgaacgc ctagaggaat ggtcggatgc
     8821 cgcgtgtgag ttttcttcat ccccagggtc ggagcggtga ggttgaatca aatctcaccc
     8881 tgttggaggc cgccaagacg ctggggtttg aactgaatca cgattgcggc ggcaatgcct
     8941 cctgtaccac gtgccgcgtt gaagtgcaga tgggtgggga caatctgtcg gagattgatt
     9001 tcgacgaaca ggacctgttg gaccgtgaag ccttgtcgga gccttggcat cggctgggtt
     9061 gccaggcaag agtgttaggg gatgtggtgg tgcgtgtgcc ggaaaccaaa tgggtgcaac
     9121 ccacatccgc ttcatcaggc gaagttggga aaagaggggc aggacttccg taagctgccg
     9181 tgagatggta gagtatcata acgtgctgaa caggtagacc agaaggagga ccaacgatgg
     9241 taacgattac gcaattggcc gagcagaaaa taaaagaact catgaccgag gaaaaagatg
     9301 tggtcggtct gcggatttat gttcgcggcg gaggatgcca cggctatcag tacggcatgg
     9361 ccttcgagtc caaaatggcc gacgatgata cggtcattga aaaaggcgac gtgaaggtca
     9421 tcatggactc tcaaagcgca ccgctgcttc agggcgcgga agtcgattat gtcgacagct
     9481 tgcaggggtc cggattctcg atcaagaatc cgcaagccaa gacgacctgc gggtgcggca
     9541 gttctttcag tgcgtaagag ccttccggtt gacccccaac cggattgcga atggtccgtt
     9601 gagtagcagc aagccgggag tggctccctc tctagcgggg ggccctcccg gcttgttcgt
     9661 tctgccgatc tttctggaga gttgtctcga tgtctcaggc caccatcacc cggcgctatc
     9721 gtttctgcgc tgcccatcgg ctgcataccg atcagctgtc ggctgaggag aattgggcca
     9781 ctttcgggaa gtgcaataat cccaacgggc acggtcacaa ctatgtggtg tttgtgtctg
     9841 tgaagggcga tatcgatccg gtcacgcatc aggtgctcga tgtgtcacgg cttgatgccg
     9901 tggtcgagcg aatcgtggtg caacgtttcg atcatcagga tttgaacctg gatccggagt
     9961 tcgcgaccca gactacgacc ggagagaatc tggtgctgct catttgggat ttgttggtgg
    10021 ataagcttcc tgcgggacgg ctagtaaagg tcggtgtgat tgaaacacgg gataactact
    10081 tcgagtattc cggctccgtg gagagcaagc ggagagaaga gggagtgagg catggctaaa
    10141 gtggtttctg gggcccgagc cagacggtcg cgtgtctctg cggcgccgac cgacgaggac
    10201 ccgattgaag ggctgatgac gaatcttttg ctggaattgg gagaggaccc tgaccgtaat
    10261 ggcctcctca atacgccgaa gcgggtcgcg aaggccatgc gctttatgac acaaggatat
    10321 cggcaggata tcgatcatct gctcaacggg gctttgttcc cgatcgagta cgacgaaatg
    10381 gtgatcgtga aagacatcga tttttttagc atgtgcgaac accacctgct gccgtttttt
    10441 gggaagtgcc atgtggggta cttgccgaac aagaaagtcg tggggctcag caagattcct
    10501 cgggtggtgg atgcgttcag ccgacgcctg caggttcagg aacgattgac gcttcagatc
    10561 gccgaaacac tgaagaaaaa gctgaatgcg catggtgtgg cggtggtgat ggaagcgcga
    10621 catctctgca tgatgatgcg gggcgtcgaa aaacagaaca ccgtggccgt gagcagttcc
    10681 atgctgggcg tgttccgcac gcaacaacag acccgcgaag agttcttgaa gttgattcga
    10741 ggcagtagcg tcagggacgg aaactaatcg cccgccgccc gtagttcctc gacgaagttt
    10801 ctcaccagat cgttaaagac ctcaggttgt tcaagattac tcaagtgccc ggccgcaggg
    10861 atcactgcca gccgggcacc gggtattcct gtcgccatca cttgcgcgtc ggcgaggggc
    10921 gtggtgtgat cttcctggcc gactaccacc agagttgggc aggcgagcgt gcggagatgg
    10981 gcgacggaat cggggcggtt cgccatggcc atcagatcga cgaggatgcc gcttaccggt
    11041 gtgtgccgga ttgtgcgccg gacgtaatca acgagttcag gtttctgttg cagtgatgtt
    11101 gcgcccagta gtttcgggag catgatgtcc gccacggcgt cggttccttt cccgtaggca
    11161 gtctgggcca gattgaaacg tcctgttctc ccctccggac tatccgcctg cgctcgagta
    11221 tcagccagca ccaatccctt caggcggttc ccgtattttc tggaaaacgc aaagccgaca
    11281 tagccgccca tcgataatcc caccaacaca gcctggggaa tagccaagtg gtcaagcagt
    11341 gcgcagacat catcggcgta gtcttcgagt gaaaatgccc acagcggggc atccgactca
    11401 ccatgcccgc gcagatccac ggcgatggtc ctgaattgtc tggacagcgc ggttgtctgt
    11461 ggcgtccaca tcgaacgatt caacgggaag gcgtggagaa agacgagagg aagtcctgtg
    11521 ccctgctcat cgtaggccaa ccgaatgccc ttcgtgtcgc aaaacatgag tacatccttt
    11581 gtgaaaagcc gatggatggg tggtagcatt gccgcgcgcg ggaatcaagg ggaggatcgg
    11641 caggtgggga cgagagtcgt ttgcgatgat tcccttgcga cagctagaat gcagggcagt
    11701 attcaggtag aagagcacga ccatgtcctc tcatgattcc acaggcgatc tcttcgcgcc
    11761 ggctcagacg gccacccgtc caggaaaagt tccgctggcc gagcggatgc gtccgcgaag
    11821 cttcgatgac ctcgtcgggc aggatgaggt ggtggggccg ggacgcctgc tcaggaatgc
    11881 cattgagcgg gatcaactct cctccgttat tttctgggga ccacccggtt gcgggaaaac
    11941 cacgttagcc gggctggtcg cgcagcacac cgaatcccag ttcgtgccgt tttcagccgt
    12001 caccggcggc attcccgagt tgcgtgagat tattaaggct gcggagcatc gtcgggcgat
    12061 gggtcgggcg accacgctgt tcgtcgacga aattcaccgg ttcaataaag cccagcagga
    12121 tgcctttttg ccgcatgtcg aacgggggac ggtggtgctc atcggggcga ccacagaaaa
    12181 tccttccttc gaactgatcg cgcccttact ctctcgctcc atcgtggtgc tgttgaagcc
    12241 gctgacagag gagtcgctcg gttccattct ggaccgggca gtggccgatt cggaacgggg
    12301 actcgggtcg ctccggatca cgttgcaccc cactgcccgc gagcagctaa ttgccttcgg
    12361 caacggtgat gccagaagcc tgttgacgac agtggagttt gtggccggcc aggcgcctgt
    12421 cgggccggat ggatcacgga tcatcgatga acgggtgctg gaggctgcgc tgctcaaaaa
    12481 ggcgcttcgt tacgacaagt cgggcgagga gcactacaac ctggtgtcgg cgtatatcaa
    12541 gagcctgcgc gactccgacc cggacggcgc cctctattgg ctggcccgta tgttggaagg
    12601 cggggagaat cctcgattca ttgcccgacg gatggtgatt tttgcttcag aagatatcgg
    12661 caatgccgat ccgatggccc tggtgatggc caacgcggtc gcgcaggcgg tggagttcgt
    12721 cggactgccg gaggctcaaa tcaacctggc gcacgggacg acgtacctcg cctcgcggct
    12781 caaggataat gcctcctatg tgggcctgca ggaggcggtg cgggatgcgc gccaacatgg
    12841 caatctcggc gtgccgttgc atctgcgcaa tgccgtgacg tctctcatga aagacatcgg
    12901 gtacgggaag gggtatcggt atgtgcatga cgatccagcc gccaaaaccg atcagaatca
    12961 cctcccggag tcgcttcgtg gcacacggta ttaccgcccc agaaccccct aattcgatga
    13021 agaaaggggc tggacaaacc tggacagacg gttatactta gggccgaggt catcatggca
    13081 cgtcaatcca gttcagcgga gagtgtgagc gagcgccgga agtatgtccg ggccacgctc
    13141 gtcgggtcgg ccctggtgtc gccgcaaagc ggagccaagg gttttacggc ggtgctgaac
    13201 aacgtcaaca agatcggcgc cggccttcat tccaaagaga ccctgcccat cggcgcaaag
    13261 attaccgtgt cgctggcctt cctggacccg gatcgcgtgg agcaacagga gaaactgaac
    13321 gccaccgtgg cttgggccaa accgtgggag aagggaatat tgctaggggt ggtgtgggat
    13381 gatctggtga cgaaagagaa gaaccgctgg ttgtactact atcttgaaga gacgctgaag
    13441 tcgaccgatt aaccgattgc cgtcaatttt tcccgcacgc cctgcaattc atcggacaac
    13501 gattcccaac gcgcagtgag tcgctccagt tcttttttcc aaccttcctg ctctcgctgc
    13561 aattcgctcc atcggccaag ttcctgatag agggccgggt cggccagctc aagctctcgc
    13621 gctttcactt tggcttccat cgaggcgatc tcggactccg cgcgcgacac ctgcttttca
    13681 aggcgcgcct gcgatttgct cagatcccgt cgttccccac cctgagattt ctgctgcacc
    13741 tgcgaagcca tggccttggt ggggccgtct gatttgcctg cttgggcggc ggcgagctcc
    13801 gcgctgcttt ccttgatcga ttcgaactcc tgcgccttct tccacaaaaa gtactcatag
    13861 tcgccgagat aatttttcgc ctgaccttca tccacttcaa cgatcagcgt ggcgacgcgc
    13921 gtcagaaacg tcggatcgtg ggagatgaag atgatcgttc ccggaaagtc tgtcagggcg
    13981 tcggtcagca tgtccaccga ggccggatcc aggtggttgg tcggttcgtc gagcagcagt
    14041 gtgttcgccg gctccaccag catgcgggcc aaggccacgc gattgcgctc gccgccgctg
    14101 agggctttga tcggcttttt ctggtcgtca ccggaaaaca ggaaggcacc ggcaataccg
    14161 cggagaaagt tcgtttcggc ctgtgcggag acctccgcca gtgactccaa aatcgtgtcg
    14221 tcggggtcca atgattccgc ctgatgttgg gcaaaatagt gaagcgtgac tccgtgtccg
    14281 acctggcgag tgcccttttc gaacggcagc acgccggcca agattttcaa cagcgtgctt
    14341 ttcccggcgc cattctcacc caccagcgcg atccgttggc ctcgctccac tgtgaagtcc
    14401 agcgagcggt aaatcacctt ttcaccgtag ctcttcgcga cgcctttgag ttccagcacg
    14461 tgccgaccgc tggccgaggg caggggaaac ttgaagcgca cgcgtttggt atcgcgttgc
    14521 cgctcgatca gcttcacctt gtccagctgc ttgatgcgcg actgcacctg cttggctttg
    14581 ttggcctgat agcggaagcg atcgatgaag ttctggacgc gagccacttc tttgctctgc
    14641 cggttggcgg ccgattcgat ctgggcgtcc cgcgccgccc ggagttcctg gaacttcgta
    14701 tagttgccgc ggaactcctg aatggtgcga tcgcggagtt cccagatatg cgtggcgatg
    14761 ccgtcgagga atttggtgtc gtgcgagatg acgaggagcg tgagattcga gtcgaggaga
    14821 aatcgctcga accaacgctg ggtcggcttg tccaggtggt tcgtcggctc gtccagcatg
    14881 agcacgtcgg ggttcgacag cagcaagtgc gcgagcgcga cacgcatgcg gtatccgcct
    14941 gaaagattct caatcggtcg cgaaaaatcg gcttccgaga agcccaggcc ggcgagaatc
    15001 cgttcggctt catgttccgg atacaggtcg cggtgtgtgg catccaggac ggtttttccg
    15061 acaatggttt cgagttcctg cggaaggtag ccgacacgga ggcgcgggcg tttgcggatg
    15121 gtgcctttat cgggagactc ctcgcccaga atcatccgga agagcgtcgt ttttccagcg
    15181 ccgttcgggc ccacgaggcc gacacgggca ccgggacgca ggtgtgccgt ggcaccttcg
    15241 agcagcactt tggtagcaaa ttgtttatgg acagattcaa gctgcagcat ggatcagtaa
    15301 gactcggtgt aaggggtgaa acgttcggtg aggtacccgc tggggagtgt cagcgtccgt
    15361 gtgtgcaccg gcggccggcc aagcgtatga ctcgtagagt tcggatatcc ccgcacacat
    15421 tcgaagcgtt ggtcggacaa gctttatttg gtggagctgg ccgggattga accggcgacc
    15481 tcgtgaatgc cattcacgcg cgctcccaac tgcgccacag ccccacgcaa gcttgtctgt
    15541 acatgtgcac aggcacggga aaaagaatca acgtgcagac gtaactctgc aatctagcat
    15601 gggcggtttt tgccagtcaa gaaaagcatt ggagtgaggc cctggcctgt cctcattttt
    15661 tgctcgtgcg ccgggcggct gcaaaaaaac tccgtgggat gggggcacga aaaggagggc
    15721 gagcatctcg tgtctgccga cggatagcag atcctcgctg aaaagcttgc ttgacaaacc
    15781 gcgaggcgaa tcctaccatg atgcccttcc ggctctgatc agtacgaggt cagggctttt
    15841 ttttcgagtt cagcaagggc agaaggagcg taggcagcga acaatggcga atctcgtcat
    15901 catcggctcc caatggggcg acgaaggcaa agggaagata gtcgatatcc tcgcgaagga
    15961 tgccgatatg gtcgtgcggt atcagggtgg gtccaatgcc ggccataccg tcattaataa
    16021 gcgggatacg ttcattttcc atctcatccc ctccggcatt ctgtatcgcg gcacccgctg
    16081 cctgatcggc aacggcgtcg tggtcgatcc cggggcgttg atcgaagaaa tggatcatct
    16141 gcaggggcag ggcgtgaaga tcggaaagaa ttttgcggtc agcgaccggg cccatttgat
    16201 cctgccttac cataaggcga tcgataaggc ctccgaacag tcgaagggcg tccgtcggat
    16261 cggcaccacg gggcgcggga tcgggccgtc gtatggcgac aagatggcgc gcatcggcat
    16321 tcgcatgggc gatttgctca atcccccgct cttcaaacag aagctggaag agaatctggt
    16381 cgatatcaat tggctgctcg aacagttgca caaagtcgac tgtttcgaat tggaaaaggt
    16441 ctaccagcag tacatgggct atgctgatcg tttaaaaagc tacatcatcg atacggccct
    16501 cgtcgtgaat aaagcggtcg atgccggcaa gaccgtgctg ttcgagggtg cgcagggcac
    16561 acacttagac gtcgatttcg gcacctatcc ctatgtgacc tcctcgagtt catcggccgg
    16621 cggcgcctgc accggcaccg gagtgggccc gacgaaaatc gatgcggtga tgggcattac
    16681 caaggcctat accacgcgag tcggtagcgg accgttcccg actgaattga ccgacgaagt
    16741 gggtggctgg ttgcaggagc gtggaaaaga attcggtgcg accaccgggc gggctcgccg
    16801 ctgcgggtgg ttcgacagtg tggtggtccg tcatgccacg cgagtgaacg ggctctcgtc
    16861 gttagccgtg acgaagttgg acgtgctgga cggctgcaaa gaactgaaag tctgtacggg
    16921 gtatcgcgtg aacggcaaaa tccaccgtga gatgccgtcc gacctgctgg cgttgaccaa
    16981 ttgtgagccg gtgtatgagc gggtgcgtgg ctggagtgct cccaccaccg gggtcacgac
    17041 ctacaagaat ttgcccgcgg aagcaaagcg gtacctcacg aggatcgagg agctcgccga
    17101 gtgccggatc gatatgatct ccaccggttc gcggcgcgat gagaccatca tcctcaataa
    17161 tccactcaag gcgggccgcc gtgtgcgtct gcaacggtca cggtaggggc cggattgtcc
    17221 gtggaacgca gcaccagccg tagcgaacta tcacgtgtca gtggtatgat ccccccgtcc
    17281 cgcgccgcgc gtgacgatgt gtcgtcgatg tgtttgggag agtcggtagc tcagctggta
    17341 gagcatcgcc cttttaaggc gagggtcctg ggttcgaatc ccagccgact caccatacaa
    17401 atcaatgact tccgcagttc ccccctctgt cgccctagtc tcataggaca atccatagga
    17461 caacggatag gctttttcga gtccttccac ggcggatctc agttttcggt cccactccgg
    17521 gcccccatga gaatagttcg ccgtcatgcc gggcatgcga tgaccaagga gcgcctgccg
    17581 gacttcgtaa tccacaccga tgcgttgaag tcgggttgcg aacgtatgcc gaaggtcgtg
    17641 gaaatgtaga tcgtgaatcc ccacccgccg acaggtttca ctccaatatt ccttaaatgc
    17701 ccgctgatga gtccaacggc ggaatactcg accatcggcg agagaaggga tctcagcact
    17761 tagcgcaagc atggccgtgc ggttcaacgg cacttcttgc ggggtgcctt tgatacggct
    17821 tgcggctagt ggcagacaca gccagtatcc atcctcacgc ttcttcatcc aggaacgctc
    17881 gatctccaga atcttccctt cccgcaggcc gaccgaaact gccacctgga taatgcgcca
    17941 caactcttgc cggcattcga gcttggtagg atcgttctct cgcatcttgc tgatgagaga
    18001 cagttcctct ggttctgcaa ttctggttcg tttctccgcg tccggcagtt cgacatgttt
    18061 caatgggttt ctatccaatt tctcataacg aacggcgaca ttgagaatcc gattgaggac
    18121 ctgccattca cgacggacgg tgccggccgt ggccccttcg tcaagacgag ctttcacata
    18181 tttcaggcca tcttccggtc tgatcgctgc gagcggctga tgcccgaagc gatgaccaca
    18241 ggagaagtcg tttctgacac aggcatggca ggacagaatg tgattctcaa gaattccttt
    18301 tggtcgcgtg cgatcgatgc gctttttgac ctcgaaagcc tgccaataca gcgggaggac
    18361 atcgcggagg gtgatgagcg aacgcggctt ctcgtgttct tcgttttgaa tcttgtgaat
    18421 caggtcaatg gcacactgtt gtgcttgttg gggcgatagg tcgtagccga gtaggggacg
    18481 ataccgttgc cccttccaac ggaaatagag gtcaaaggca atccctttgg ttgtgcgtcg
    18541 cttggtaatc gtataagcca tagtcatccc cgatattttg ggttccgatg gtaaacagtg
    18601 gtatgagccg tgtctagtgg caaaaccagc tcgctagaat ccgccgactg tgctctccca
    18661 tcaccacttt tcaaccaggc gattagctcg tctttgagaa atctgatttc tcgcgatccc
    18721 ggcatgcgat gcctcggaat atccttccgc tgatacagcg tggattttga cacacggaga
    18781 tagaccgccg cttcggccag ggtcatgatt tccagcgtcg cgctggcttg ccgttcctga
    18841 ttcattcttt tctcgctctc actgaaacac ctctgcactg tccggcttgg gtcgaagagt
    18901 gagcccccag tgggtacgac tcggggccgg ggagcactgg gacagtcctt ccgtgtatcc
    18961 cccctccagg cctgcccggt gcgagagccc cgtgccgcct ccttcgttac gccccaccct
    19021 tggttggtga agggtggggc tagaacataa tttcagaagg aggcagacat gtccgttgat
    19081 agctctctcg gttcctcgaa cggtggacga ccagtgagcc cggttcggaa atacggaatc
    19141 cctcggtacc gtgtcacgct cgtgcgtgag ggccgtgccc ttccggcagc ggaatcggtc
    19201 catacgtcgg aaggtgcggt cgccatcctc cggccgttgt tttccggtct ggaccgggaa
    19261 caattcctca tctgtggcct ggacgccaag cacaagctca tcggaatcaa tgtcgtgtcc
    19321 accggctctc tgaacctgac gattgtgcat ccacgcgagg tcttcaaacc gctcattctc
    19381 atgaatgccg gtgcctggct ctgttcgcat aatcacccgt ctggagacat cacgccgagc
    19441 ccagaagatc gcatcctgac caaacggctg cgtgaagctg gtgaactctt cggcatcacc
    19501 ctcctcgacc atctgattct ggccgaggag cgctactaca gcttcgccga ccaaggctgg
    19561 cccggcgctt agcgcaagag ccgcaacacc caagcagcgc cggccgccac gagcgctagc
    19621 ccgatcgccg cagtccccca ggcgaccagg tccacagcca gtgcattgcc gatcgtttga
    19681 atctgttgcg ccgtcattct gcctcctcat tgtcatcgct ctcagcctgt tctcgtttct
    19741 ggatgaaaaa agaggcaggc tgctgccagc ctgcctcgat gggctcgcta gcccacaatt
    19801 gcgcgtacgc gcttaaaggc gtaaatcgcc aacgccacgc caatcaaggc cgtggcccac
    19861 aagagcagat cggcccgcac cgtggccaca tccgccgaca ccggaaacaa ctgcgcctgt
    19921 gagagcgccg ggacacccaa ggcgaacaca aacgccatgg gcagccctcc aagccctttc
    19981 acccaacccc atcccttcat acggcacctc cttgtgctgg tggtggttcc gtccacccac
    20041 gccaccggat gactcgccgg cggacgagcg cctcagtaag tctgaggcag aatcaacttg
    20101 atgatcaggc cgacggcaaa accgccgagc caaaaaatgg ccgtgaaata ggtcatcgcc
    20161 tcaatcgccg gcaggtccat agaacactcc ttcgccaatc agtcgcatcg atccttcttc
    20221 gtacacttcg gagaccgaca ggccatagcg cccggtaatc tgcgcggcgg tcaggatctc
    20281 gccgctgtcg aggagatagc gccacccttg acgatcctta gtcatcccag cgccaccgag
    20341 gattctcacg ggtcgcttcg gaacagcggg agtggctggt gccggctgag acattcccaa
    20401 ggggttcaac agactggccg aaccggatgc tggtgcgccg atcgtggtgg ttgggacagg
    20461 agttgatcca gctactgaag atgccttgct cgcactcaac gatgaccagg gcctccagac
    20521 catgagacca atggcaaaga gtcccgccgc aatcccaatc gcgacccgtg cggacttgaa
    20581 gacggtatgg ctccgtttct cttctcgaat cgccgccgag gcgtaactcg aatagtagga
    20641 gtagatcgcc ggcgaatagg tgccgacgaa ggcccggatg acctcgtggt cttcagggtt
    20701 accgcgcacc ttgccctgat acttttttga gagcccgaca aaggccagct tgcgaaactt
    20761 gaccgtcgct tcaatcagcc gtgtcacgcc ttgcgacatc tgacggaaat cctggctcat
    20821 gagcagaata tccacgccat aatgccgatg cgtttcgagc caccgcagca gccctggctc
    20881 gaccttctgc atggaccgaa agacggtctg cgcttcatcg atgatgaccc ccgagccggg
    20941 ctcgacatgg gcaaaggcct ggagaacctc gactgagtct ttccagagcg tgatctgttt
    21001 ttcgaggata gtaagctcga tgccggtgaa cagggacagc cgatccagat agatgccatc
    21061 gactgcgata tagagccgcc gtccttgttt gacccacggc aagaacttct cgcagatcgc
    21121 gtgatacgac ttccccgagc ctggtacccc ttcatacaat tcgatcatcg aagactcctg
    21181 aggtagaccc aaatccagag cgctaaaatc accgtccagg cataggccca gcccaccgcc
    21241 agctgattca tgaaccccac cggacaaacg ggatggtttg cagaatgaat cgggtgccca
    21301 tggcgctcgc cacgatggcc agcgcctggc tcatgcccgt tgcgcccagc acccacgcat
    21361 attgatcggg aattaccggc aaggtgagac ctgccgtccc aatggtggcg agcgtgctgt
    21421 ccgccaccga gagcaatgaa tcccaaatcc cgaggcccca atcggtgagg gagaaaaaaa
    21481 actcctgcag ccagcaatag atcagcgtca gaatggcggt catgtcgtct ggcctcctcc
    21541 cacaaagatg atgcggtagg cggcaatgga cgccgtcgcg atgaccaatg ttctgagcac
    21601 cgtgaagaac caggcccatt gattgaaatc gacctgctgg ctgccaaaga aggccgacgg
    21661 aagcgcaatc accggcaagg tcgagggcca ggtgagggac ttcaagagat tcaatgtgcc
    21721 gagtaaccca ctcatctccc acgtggtttg atgcgcttgc agaaccgtgc cgaaggttcg
    21781 actctcatgg gagccgaccg cacaggaggt cgtggcttgc gtctcttcgt gctgcgtcgt
    21841 ggagccgtcc ggattctgcg tcgtggtggt cgtcgtcgtt gtcgtctgct gctgggtatt
    21901 ctgctgcggc gtactggccg gaggcgggac ggtgtccact acgacgatat cgccggccgg
    21961 aacaggtttg ggcttgacgg tcgtgggcat ctcggtcggt gtgaccgcct gggagatggt
    22021 ggtatcggcc ggctgtgtcg tgccagtggt gccgaccgga ttggtgtgag cttcgacgga
    22081 cttaggatcg ctggccggaa ggcccccgac gaagttggcc acttgctgtt gggtcggcgg
    22141 aatcaaaccc tcttgaatcg gtgaggtgga gccgggaatg ccttttcgat ggcacacata
    22201 caaggtgtat ccgccgacgg caggaccatt cacaaagaag ttgccggtga agaagacggc
    22261 cgtactgagg ctttgaaacg gcccgacgac ccaatcatgc tgatattccg tatcgaccgc
    22321 gcagagcggg acattggtcg cgctgaattg aatggtcgcg ttgggatagg ccggattgcc
    22381 tggcgtattc gtgccgaaac ctgggaacgt ctgtgttcct gcattcgtgc tggccacttg
    22441 ccagccgccc ggcgtggatg cagcggtttt cacggccgag aggtcagttt gtgagtaata
    22501 catctgggcg aggaccaatc cagcgctgac ccctagcgct gcccatccga cgggaccggc
    22561 gaccatgcgt acggccatag aagcggcgga aggtgccacg gctgctgttg caacttgaga
    22621 agcgagggcc gagcgttgcg cggccagata cgcaatgcgt tcggcttgcg ccacgacacg
    22681 ggaatactgc gtcgcgggtt ggctgaaggc ttcccccggc gcaacgcaaa atgagacgag
    22741 aagggcccag acaaacccga gatacgtcat gagagacagg actttcacaa ccgtccgact
    22801 ccaaggccgg cgaggaaggc cagtaacaag accgccacga gtagaatggt gagatccact
    22861 ggccttcctc cccctgcgat tacttcagga cttccaatcc agacaaatcg aagaagacgc
    22921 ggcctgtctg ttcgaacttc cgcacttcga tcgacgcgcg agcctgtttg ccttcggcct
    22981 gtttgcacac ctcgatcaat ggcatctgat cctccggaat ccccaaacgt agcacccccg
    23041 gatctttgcc cttcacatag aagtccaccg atctgtagac cttgccttcc cggctcctcc
    23101 gttccacata gccctgcact gcgccctccg ctttgacttg catcgtcgag acctcctgaa
    23161 tagtgagtga atttcgagtc agccaagcgc ctgacccaca cgccccggcc ttcggaccgg
    23221 gcttttggct tcaaacagaa ttccgaatcg catgccgtac agctcagata cagagcgccc
    23281 tgtagccact tgcaccgaca gcctctggtg ccacatcctg gacacatgcg tcgccacgac
    23341 ccgttatgaa actctaagga catagggggt ccctcgcctg cgttgcttca gcagggcata
    23401 gtgcttctgt gtccatcgtt tggtacccgc atagatcatc tcgaacagaa actgatcccc
    23461 gcgacaggcg acgaccaccg cgagcatggg actgatggcg ttcgccagcc atgccgccac
    23521 atcatccagc cgctgctgaa tccgttcgac aaccagccgg catcgcatga agccctcggt
    23581 gagggctttc caccagccca ccaacggcgc gcgatacttc tcataggatt ccgcttctcg
    23641 cgtggtctcg cgaaaatcga cataggaacg cagcacgcca accaaaaacg cccgccaatc
    23701 ttcgcgatcg agcgtgagga gggctttggc acaggcctga gcccgatcct gtttgaattc
    23761 gagttcccat cggacgccat acaatcccgc gtcttctcgg cctttggctt ggagttccag
    23821 ccgcttgtcg tagacgcgca acatgctttg gctttcccga ctgccaaagt agagcgtttc
    23881 tcccgtccgg acgccttcac gatgattcga ggcctggatg actttgaatt gcttcgaacg
    23941 actcaccaat tgtccggcct ccacggcgag gcggacggtc tccactgcga cagaggcctc
    24001 ccggtcgtcc agcgccacat cgatacgggt gacatggccc ttttgggaaa agatccactg
    24061 gaggactgat ttcagtttcg tctcatccca ctgggagaca atcccggccg agagatccac
    24121 atgcacttcc ttcggattgc gaggagcacc cgtccccagt ttgccgacgc ccgttttgcc
    24181 ttccgtcatg agttgcgcca cgggataccc gcgaaagccg gtctcactct ggaaccagtc
    24241 gcccccaatc agcgcaatca cctcggcgac ctccgctttg ggcagggtga aggcgagcca
    24301 atcgatggtc tgtgtgaacc ctccagaacc gttcatttct tcccccttgg cttctgtgcg
    24361 atagacgccc ccgtcttacc aagtcggggg ctggtccgct gcgcgcgccg ccggctggcg
    24421 ccgccggtcg cgctgcgctg tccctgtgtc cgtgaggaga aaggagactg tccttgcccg
    24481 ttgccctgca tcgcttcttt cacccgttgg aggtcgaagc gcacaaatcg gcagatccgt
    24541 tccactggaa tctcgccctt ccggtaggcc cggcggatcg acttcacgct aactttgagg
    24601 acagcagcca attcatctgc ggttaaccac gacgttttca tgttgttcct cttacgttgt
    24661 tctcgcggcg cacggtgccg accataccga agcgcggatg agtggtgaaa gatcccgttc
    24721 agggtcacaa ggggacagga ggggacagca agggacagta gttcgcggca ggagtgctct
    24781 ataattcggc gcgattcacg aggagtcgga gatgtttcga cccgtcctcg atctaacatc
    24841 acgaaaatga aaactcacaa ataccgctct acatgcggag aattggcggt agcccgtatt
    24901 gcctctagaa ctgtcgagat ataacgacat ttgaaatccc atcgtaagcg atcactacgc
    24961 cgagctcttt ggaagcgtgc tttagttaaa aataagcaat agggccgaaa aacatagacc
    25021 agggatgaat ggccctatcg ctaggctgct tggctcccag acccgatgtc cggatgaaat
    25081 tggaaatggt ccgacgtagt gcaaacacgg tgcgagaacg agcgcggttc gaacgtggcc
    25141 taatggatag caacgaggag tgctaagagg gtgtctcacg gatgggtagc gaaaaaaagc
    25201 ccgaggagag tcaaaaaatg attgcggcag gcgaccagat tgtcgctcat gtcactgccg
    25261 ggaacccgag actcgtatgc agtccgaaga gccaatcaga ataggtttca cagcgaaggt
    25321 cgcacaacgc tcgattccac ctcacctacc aattaagtcg gttatgcaaa agctttattg
    25381 tgaattctct ctctgccgag gccagtgatt tctaacggca aaccaggtat tctgcagcga
    25441 taaaactgct ggttgtaatt atcgtcctac ctgagatcgg caagccgcta cagataggaa
    25501 gccttgatct ctatctaacc ggtgataggt cggagggcat aggttaccaa tgatggtgga
    25561 ggcactggct gaaaagcatt atggggctga gctgacagct ggagcattaa aagttccaga
    25621 gagccggatt attgctgatc tgcttctccg aggcgtcgcg cctgaagaat ggcatgagac
    25681 tctttataag cagaatgcac tccaaacccg taaccttgag acagcgaggc gactagggag
    25741 actgatccgt cagcggctgg agttaatgaa tgtcgacctc tggcggctcg ttcgtgatgg
    25801 ctcaaaaacc gtggctacgc acgcgtgtct cgcggctgcc attaagcaca gtgccttgct
    25861 aggagacttc ttagatttgg tcgtcagaga gcaataccgt cttttcgcct caaaactctc
    25921 accaacgctc tgggaagagt atctgaatga ctgtcgaggt cgtgaccctg agatgccact
    25981 ttggaacgac tcgacgagaa ggcgcttgcg gtcgtcagtg taccaaacac ttgcacaggc
    26041 aggatttctg gacagcacac gaacactacg gcttcaacca gtccatatcg cctccgaagt
    26101 gcttagttat ttgcgggacc accgtgaaga gtatgtgctt cgctgcatcc aggtgtctcc
    26161 gtgagtgatc tgttgaacga tcggcttaac aagattcttc cacggatcat ctctgatgac
    26221 tttctgagcg gcagcggcat agggaacgag atatcttttt acattttcga ctacccaccc
    26281 gaggaggagc tccgagtacg agctcacctt catttcttag aggaccacat tcccaagcaa
    26341 aaacctgagc tgcgtttcac gcacatcaac ctgttcgatc tagtgctgga ccacctcaag
    26401 cgccggaaat tgctcgagaa ggcactacag atacaacggg agaagggtga cgaggcgctc
    26461 aaggcagcgt tggctggccc actccatcca cagaagctcg catcgatctt ttgtgaagtg
    26521 gctaggccgg accagaacaa acttgtcctc gtgtcaggtg tcggaagcgt gtatccgttg
    26581 ctccgaacca gcggcttgct cagcaatctg cagaatgtga tgggacccat tcctctcgtg
    26641 ctgttttatc caggaaagta cgatcagctc accctcagac tgtttgggaa actgagcctg
    26701 tcggcacact tggacggtat gtcaaaaagc aagaagcctg agcattatta ccgcgccttc
    26761 aaattggtgc cctaacagag gctgactatg aagattcaga atctctttgc ccgagacctg
    26821 ttccgctcca tcaacggagt cgtcaaggcg gatcagttgg acgaatcgtc cgtctggcag
    26881 gagttggacg aattcgttgt gacaagagaa ctcgatcagc atctccgccg ctttttctcg
    26941 acatatggtg atgcgatcaa gcacccccaa gatcccgatg tcgcagggaa gattggtgtg
    27001 tgggtgtcag gcttcttcgg ctccggtaaa tcacacttta ttaaggttct gtcgtacctc
    27061 ttgcagaaca agacccatac gtatcaggca cagagcaagc gggcggtcga gttctttgag
    27121 agcaagatca aagatgccat gttgtttggt gacatcaagc gagcggtcgc ctccaatacc
    27181 gatgtcatct tgttcaatat tgacagcaag gccgaccacc gtattgggcg cgatgccatt
    27241 ctttccgtgt tcctcaaggt gctaaacgag atgcaaggct attgcggtga tcatcctcag
    27301 atcgcacatc tggagcgtca cctggaaggg aatggcaagc tggcacagtt ccacgacgcc
    27361 tttcgcgagg cgtcgggtag cgaatgggtg gcaaaccgtg atgcatacca ttttcatcgg
    27421 gacgaggtcg taaaagcgct cggcgaaacg ctaaagatga gtaaagagtc ggcggaaaag
    27481 tgggtcgatg gggcggaagg cagttttgcc ttaaccgtcg agaatctctg taaatgggtt
    27541 aaggaatatt tggactcgaa gggggaagaa catcgtctta ttttcctggt cgatgaagtg
    27601 gggcagttca ttgggacaga ttcacacctc atgctcaacc ttcagaccat tacagaagag
    27661 ctgggaacca tctgcaaggg ccgggcatgg gtggtcgtca cttcccaaga agatattgat
    27721 gccgtgctgg gcgaaatgaa gcagagtaag gccaacgact tctcgaagat tcagggacgt
    27781 ttccggacgc gcctctcgct ctcaagtgcc aacgttgatg aagtgattca gtctcgcctc
    27841 ctggcgaaac gcgaggaggt caaaagcaac ctccaggaag tcttccgcaa aaaaggggac
    27901 attctcaaga atcagctcac gtttaccaac tgcggaatga ccttcaagag catcaaggat
    27961 ggcgaggatt tcgtccagaa ctatccattc gttccgtatc agttccagct cgtccagaag
    28021 attttcgagg cgattcgaag agcgggggcc accggtctgc acctctcccg tggcgaacga
    28081 tcgatcctcg atgcgtttca gtcggctgga aagcaggttg cccttgaaga ggttggcatt
    28141 ctggtcccac tctatcggtt ctacccttcg atcgagagct ttcttgatac agtcgtcaaa
    28201 aagacgatcg atcaagccaa ggacaatccg agtctggagc atccgttcga tatccatttg
    28261 ctgcaggtct tgtttctcat ccgctatgtc gaggagatca aggggaacgt cgataacttg
    28321 gtgaccctgt gcatcaccga gattgatgcc gaccgactgg cgcttcgtcg caagatcgaa
    28381 gggagcctcc agcgtctcga aaaagaaacg ctcattaatc gaagtggtga tctgtatttc
    28441 ttcctaacca acgaagaacg ggacatcaat cgggagatca agaacgtcga actcggtagt
    28501 ggcgaggaag ccaagctcct tggtgaactt atctacaacg aggtattgaa ggaacaacga
    28561 aagtatcgct atcccgacaa taagaaggac cttgagttta atcgattttg cgatttactc
    28621 ccgatcggga accgctttga tggcgcgtta caattctcgg ttatcacgcc actcaacgat
    28681 gagtacgagc tatatgacaa cgccaagtgt ctgctcgaca gctcacctga aggaggcagc
    28741 attctggtcc gcttcggcaa tgacgagact ctgggccgcg agctccggac atacctccag
    28801 actgagaagt acgttcggca taagaacgat ggcacacttc cagagtcggc gaagcgcatt
    28861 ctccgcgaca tttctggaaa caaccaagac cgccgagccc gtctcgtcac actcttaggc
    28921 gagatgttcg ccggagccga cttctatgct gccggccagc ccttgaagct gaaagcctcc
    28981 acgccattag ccgcgctcga cgaatcgctg gggtatctcg tcaagaacac cttcaataag
    29041 atggggtacc tgaagcggtt gtctcaggag ccattaaagg aaattcaggc catcattcga
    29101 agtaacgaca tcggtcagca gacgctcctc ttggaaaccg aggagggcaa caagcaggcg
    29161 atagaagacc tacgcaatta tgtcgccctg atgtcatcaa ataataagca aatcgtactt
    29221 caggacatga tcgacaagcg gtactcgctg cgtccgtatg ggtggccaga agacgaagtg
    29281 gtgatgctga ttgcacggct catagtgctg agtgaaatca gtctcatgat ggatgggaca
    29341 ctgatcccac tcgagaaggc ctacgacgcc atcacaaccc ctgcgaaacg ccgaaagatc
    29401 gtgatcctca agcggcaaac caccgacccc aaggccattc aaggcgcccg aacactggga
    29461 aaagaattgt ttcatgagat gggacctgat ggcgaggatg ccctctttgc attcctgcaa
    29521 aagaagctcc agagctggca atcatccttg acgagctaca agccattggc ggagaccggc
    29581 gattaccccg gtagagacca gattgcggac gggctcatgc gtgtaaagaa gctactcact
    29641 tccgataaca gctataagtt catccagtac ctcaacgaac agaaaaatga acttctcgat
    29701 ttcgccgatc actttaccaa tctcgaacag ttctatgaac accaaagacc cacctgggag
    29761 aagctacgga aggcctatgg ccgttttcag cggaatcacc ttgagcttga gagagatgca
    29821 aaggctggag tggcgctcaa acggatgcag caaatcctga acgcgtcgag tccttacagc
    29881 ctaatcaaag aaacggaggg gctcattgcc acagttagtg ccgtcaatag cgccctcgtg
    29941 gctacagctc gccagcaggc tcttgtcaag attgacgggc accttgccac aattctcaag
    30001 gacatcgaag tagccatggg tgatcctcag ctgcgtgacg cctgcatgag gccattagaa
    30061 gcactgaaga gcacagtcca actggaagag agtctggccc acatcacaca gatagaaact
    30121 gaggctctga aagaattcga tattgcaagc tctcggatag aagaatctgt tgggagagcc
    30181 tcagagcagt ccaaagaaga gcagcccaaa ccggtgctca gaaagaaacg ggtagtggag
    30241 ccggccaagc tggtcgcatc tccctatttg gaaacgcagg cggacgtaga agcatttctt
    30301 aagacgctgc gatcggagtt ggagcaggca cttacgaagg atgaacgcat cgagatccgc
    30361 tgagaagaga acgtcagatt ggaaatatga accgcaccaa gctcaagatc tacgccccgc
    30421 aagctcgccg ggatttcatt caggccgtgg cagaccgtgc tgccttctat ggtctgactg
    30481 ataagaagat cgaacccatc accgagaacg gtgacatggc catcatcgga ggccgcgcct
    30541 tccctcgctc tattgcggcc aaacgtaagg ttctggaaga acggattgcc cagtacggct
    30601 tcgagcagac gacggacgca atcgcctaca cctggttcaa ccgcctggtc gccatccggt
    30661 acatggaatt gcacggctat cttgaccacg gctaccgggt actgagccat cccgatgcgt
    30721 cgaagtcgat tccagagatc ctggaacaag ccgaacacct tgatctggcg ggcttggata
    30781 agcacaaggt tattgagctg aaattggaag gcagcaaaga agctgagctc taccggatgc
    30841 tgctacttgc tcagtgcaac gccctgcaca agacgatgcc gtttctcttt gagtggatcg
    30901 acgatgaaac agaactcctc ttgcctgaca acctgctcca cacaggctcg ctgatccgaa
    30961 agcttgtcac tgatatcgat gaggaagatt ggcaggaggt cgagatcatc ggctggctct
    31021 accagttcta catcgctgag cgtaaaagcg aggtcatggc ccgcaagagt gctgtgccaa
    31081 cggaggacat ccccgccgtc acgcagctct ttacaccgca ttggatcgtc cgatacctcg
    31141 tcgaaaactc gctcggacgc ctctggctgc tgaaccggcc cagctcccgg ctacgtgagc
    31201 acatgcccta ctacattgaa ggcgaagccg agaccgattt ccttcggatt aataggcccg
    31261 acgacatcag gctgctcgat cctgcctgcg gaagcggaca catgctcacc tatgccttcg
    31321 acctgctctt ccgtatctat gaggaagaag gctacgtgcc taacgagatt cctgggctca
    31381 ttctgaagca caatctcaat ggtctggaaa tttgtccccg cgcggcgcag ctcgccgagc
    31441 tggccctcgt ctttaaggcc cgggcaaaat cccgtcgctt cttccagccc ggcactctag
    31501 tacagccccg catcctggcc ctgcaagata tccgctttga tgaaggcgaa ctccgcgact
    31561 acatcgcggc gctggacctc ggtgccctct tcaaagagcc catgctgcgg ctgctccatc
    31621 aattcgagga cgccaccacc tttggctcgc ttatccagcc ctgcctggac gaacagaaca
    31681 tcaccttcgc ccgtcacgcc attgaggcca aggacctcgg tggccagctc tttctccgtg
    31741 agacccaccg caaagtgctg cgcgctctcg aacaggcagt gatgctgggc aagcggcatc
    31801 acattgtggt ggctaatccg ccctatatgg gccacttggc attcaactct tcattgaagg
    31861 ccctggcaaa agcatgcttc ccattaagta aagaaaatct gtattcgatg tttatgcaga
    31921 gatcactgga actgacaatc agtgatgggt tctgtgccct tgtaactatg aatagttgga
    31981 tgtttttggt taagttcgta gaattgcggg ttagtctttt gaagagtgta actatcgagt
    32041 gtatggctca ctttggtcca cgagctttcg atagcatcgg tggtgaggtc gtacaaacga
    32101 cagcgtttat tttcctagcg aggtcaattc cgactttcag aggaagctac cagcgactta
    32161 tagatggaaa atcagaagca gaaaagaatg aggccttatt aaagcgcgca gccatttatc
    32221 gtgtttcgag ccaggaatta ttactagttc ccggcgcacc gattacgtat tggataagtg
    32281 agcgaacccg ccaaatattt gcaagcgaca agccgttgga agccgtggct gaaattcggc
    32341 agggtatggc tactactgac aacaaacggt ttcttaggat ttggtcagag atctcttttt
    32401 ccagtatcag ctttggaaat atctcacgaa accaagcatc cgaatcagga aaatcttggt
    32461 tcccatataa caaagggggc gagtttagaa aatggtatgg aaataacgaa tacattgtgc
    32521 tgtggcaaaa cgatgggcag gtcatgttcg atctagtccg caagaaatac ccaagaatct
    32581 ccgaccctga attcgttatc aaaaatccaa acacgtactt tcaagagagt attacatggt
    32641 ctgggattag tacttcttct tttggcgcgc gctatagtcc acctggcttt atttacgatg
    32701 tggttggatc ttctgttttt ccgcataata aagactactt tactcttctc tctctttttt
    32761 gctccaaaat tgctgcgcac tttgtccaga ttctaaaccc aacttaccat tttcaggtcg
    32821 gcgacgttca gcgaattcct tgggaacctg aagcgataga gcgctgccaa gatacgactt
    32881 cgctagttgc tcgtgaagcc gttcgagttg ccaaatccga ctgggacaat tttgagactt
    32941 cctgggattt caccgaactg cctttattgc gagtagggac gaagggtacg acgctgtccg
    33001 catcttggca ggcatggagc gaccagtgca ctgccgcaat tcaccgcatg cgtgacctgg
    33061 agagggaaaa taaccggctc ttcatcgacg cctatggtct tgccgatgag ttggatccag
    33121 atgttcgaga agacgaaatc accgtcagcc gtagtgatca gccgaaagac gttgccgcat
    33181 tcctctcgta tgccatcggt tgcatgatgg gtcgctactc gctagacaag ccaggcctta
    33241 ttctggccga tgccggtgat acattggagc actatctgac caaggtgggc agaccaaaag
    33301 aacagctctc ctttactcct gacgaggatg gcatcattcc cgtactggac cgagaatggt
    33361 ttgcagatga cattctggcc cgtacggagg cgttcctgtg tgcgactttc agagaagcca
    33421 cgcgggaggc gaacctgaaa ttcatcgagg aatcgctggg caaagacttg cgcaaatatt
    33481 tgctgacaga cttctacaag gaccatctcc aaacctacaa gaagcgtccg atctattggc
    33541 tcttctccag cggcaaggag cgagcgttcc aatgtctggt ttatttgcac cgataccagg
    33601 acggcacctt ggcccgtatg cgcacggaat atgtcatccc gttgcaaggg aaaatggccg
    33661 ctcgcattga acaactggct tctgagaccc acgaggcggc ttccacttcc caacgcaagg
    33721 cactggaaaa ggaacgagag aagcttttga agcaccaagc tgaactccgg acatttgacg
    33781 agaagctgcg gcactatgca gatcaacgga tcacgttgga tcttgacgac ggggtgaagg
    33841 tcaactatgg gaagttcggc gatctgttgg ccgaagtgaa agcggtgaca ggcggaactg
    33901 acgaggactg atggatactc gccaaattca tgacacgctc catcgtatgt ataaggaaga
    33961 gggggcgagg attgtcttct ggaacgatcc cgacaaagag ttccagacta ttctgcctgc
    34021 gctgaacctc gacggggtga acatcatcca gctcaacggg gtaggtgctt tagaagtaaa
    34081 gattcgtgta gagcgggagg atcccacagg gcggtatctg ctctattccc caagtgagga
    34141 gcccgattac gagaacgatt ggcttctcga cattcgcctg tatagtcgcc ctttcagagc
    34201 cgaccgcgca tcgattctcc tcaatgagct tggactgaag aacctccacg ttcgtcagca
    34261 tctggctgac cgtcggaagt tttttgacaa caaagaacgg ctccagaagc tgaagaactt
    34321 ggtcgagccc aacgatatag ccagcgacct ggatcgaaaa atgatcgcca cagtcgtgaa
    34381 ggcagaccag ccggaatggt tcaacatcat ccgtacgctc taccatgcct atactgacaa
    34441 tggcgatgag atcgacttgc agacggaacc ggcagcttgg gaacagatcg agaagtttga
    34501 gttagacaag ccattctggg agatggccaa caccacgttc ggctattccg aagccactcc
    34561 gacgctcaag aatcttctcc tcagactctt cgcgaccgac tatgtgcatc atctgaaggc
    34621 cgctccgcca aatgcgcttg tgcatctgat cctcccggca caaggacgat cgaatgccgt
    34681 ggtctgcctc ggacaatggc gcgatagcag cagtaaaggc agtagttatg accggctttc
    34741 cgaagaagtg gcggacctaa ttcacctaga agattacttg cggggcttgg agatccctga
    34801 cctgctcgat gtgatgacgt tccttgtggt ggaacgcggg attgccagcc gtctgcgtga
    34861 gcgggtgcat cagacggcgg acatgatcaa ccccgatgaa gttcggtcaa tcgcgacccg
    34921 ccggcaggct ggacactggg cctcgctcat ggtgaacggt tccccggcgg ttccgcgacg
    34981 ggcgcttcat gcggtgtatc acgctctggt tgccgccgct gatttctttg gtctgcggaa
    35041 tcagcatcgg gatggattcg tctttcacga tccggcggct ctctatcggg cctatgagga
    35101 cgagttattc cgattcgacc agctgtatcg gcatttctgt gaggccgctg atcatgcgga
    35161 ggcacagggc tggaacatcc tcaagccgtt gcgggagttg atggaggatt gttatgcgaa
    35221 ctggtacctc gcgacgctcg cattagcttg ggggaaattt cttgatcccc aaggcattgc
    35281 ccaacttctt aacaagtggc agctcgaaca gattcccaac caacaggact tcttcaaccg
    35341 acaggtgcgc tctcgcctgg aggaagctga gaaccggaag gcgtatgtca tcatcagcga
    35401 cgcgttccgt tacgaggcgg cccaggagct tacccaagag ctcaatggaa cataccgctt
    35461 tgaagcgacg ttgacttcgc aactaggcgt tgtgccctct tacacagctc ttgggatggc
    35521 aagcttactt ccgcatcaga gtctttcgta taagcagaac ggcgatgttt gggtcgatgg
    35581 aaagcccaca tcatcaaccg aacagcgagg ggcaatcctt gagtcggtcg aaggaatagt
    35641 ctgcaaggcg gacgagttgg tggccatgaa aaaggagcaa ggccgagaat tcgtggcagg
    35701 taagcgcgtc gtctacattt atcacaatgt cgtggatgcc attggtgatg ctgcagcgac
    35761 tgaggaaaaa acatttcaag gggtgcgaaa ggcgatcgaa gaacttgctg cgctggtcac
    35821 ttacatcatc aacaacttga atggcaatca tgtcttgatt acagccgatc acggattcct
    35881 cttcacagag tcgcctccag gtcaaccgga caagaccaaa ttagaccaga agccagaggg
    35941 aacggtccga tcgaagaagc ggtacgtgat tgggcgaaag cttcccgaca atgactatgt
    36001 ctggcacgga aagacctcag tcactgcttg cgctgaggga gaaatggaat actggatccc
    36061 gaaggctggg aatcgcttcc atttcactgg tggtgctcgt ttcattcacg gtggagcaat
    36121 gttacaggaa attgtcgttc cggtgatcac ggtcaagcac aagaaagata aatccactcg
    36181 agaggacacc aaggtcaaat ccgtaatcgt gcatgtgttg ggcgccagcc acagagtcac
    36241 aacgaaccgt catcgcttcg aactgatcca ggcggaacca gtgagcgaac gagtgaagcc
    36301 gatcacactg aaggtggcga tttatgaggg acatagcgcc gtcacgaatg tcgagaccgt
    36361 gacgtttgat agtgcctcag ataaggtgga cgaacgtaaa aaatgggttc acttagtgct
    36421 gaaagatcat cagtatcaca agagcaccgc atatgggctc atccttcggg atgcagacac
    36481 tggaattgag caacagagtg taccggtgac cattgatagg gtctttattg atgacttttg
    36541 aaaacaaagc gatcgatacc aacctcgacg atctcctcaa tcgccatttt gccgggaagg
    36601 tcgtccgcaa agatctgacc aaattggtta aagaaggggc gaatgtccct gtatatgtgt
    36661 tggagtatct cctcggcacc tactgcgcat ccaccgatga gaccatcatc aaagaaggcc
    36721 tcgtcacggt aaagaacatt cttgccgaga actatgtgcg tcctgatgaa gcggagaagg
    36781 tgaagtccac cattcgtgag cgtggaagtc tcaagatcat cgacaagata acagtgacgc
    36841 taaatgaaaa acgagacgtc tatgaagccc tactcgcgaa tcttggagtg aaaggggttg
    36901 aggtcagcac cagctacgtc aaaaagtttg agaagctgct cgtgggaggc atctggtgca
    36961 ttgtgacgct gcagtatttc tatgaggaga accagaaggg ttcaccattc gtcattcagg
    37021 acctgaagcc gatccagatg ccgaacatgg atctggagga gctctttcaa ggccggcgag
    37081 cgttcacaga agaacagtgg attgacgtct tgttgcgatc aacaggcatg gagccaacga
    37141 acttcagcga acgcgtgaag tggcacttgt tggcccggat gatcccttta gtggagaaca
    37201 actacaacat ctgtgaacta ggaccacgag ggaccggcaa gagtcacatc tataaagaga
    37261 tcagtcccaa tagcattctt gtctctggtg gccaaacgac cgtcgcgaat ctcttctaca
    37321 acatggcgcg acggacagtt ggactcgtcg gcgtctggga tgtcgtggca tttgatgagg
    37381 tcgctgggat tacgtttaaa gaaaaagatg gcgtccagat catgaaggac tacatggctt
    37441 cagggtcttt ctcgcgtgga cgagatgcca tcaatgcata cgcctcgatg gtgtttgtcg
    37501 gaaacatcaa tcagtccgtg gacaccctcg taaagaccag tcacctcctg gcccccttcc
    37561 cagatgacat gatcgacagc gccttctttg accggttcca tgcctatgtc ccaggctggg
    37621 aaattcccaa gatgcggccg gaattcttca ccaaccagta cggcatgatc gtggattacc
    37681 tcgccgaatg gatgcgcgag atgcgtaagc gaacatttgg cgacgccatt agtaagtact
    37741 tcaagctcgg cagagatctc aaccagcgtg atacgatcgc tgttaagcac accgtatccg
    37801 ggctgttgaa gctgctgtat cccaatgagg aatacaacaa ggacgccgtt cgtcgatgtc
    37861 tcgaatatgc cttggaagca cggcggcggg taaaggagca gctgaaaaag atcggcggaa
    37921 tggaattctt tgatgtgcat ttcagctata tcgatctgga aacgttacaa gagaagttta
    37981 tcagtgtgcc cgaacagggc ggaggcacac tgatcccaga tggtccgatg agcccaggag
    38041 cgctgcacac agtggccact ggaagcggag gtcacttagg gttatatcga ctggagactc
    38101 aagtgacggc gggaaatggg tcgctgaaga cttccggcct gggatcgaac agcggagcga
    38161 aagaggccat caaagtcggt tttgacttct tcagagcgaa tgcctcgcgt gtcagcgctt
    38221 caattaaagc aggcgaccat gactatcatc ttcacatagt ggaactgcac aatactggcc
    38281 ctacaaatgc cctgaccctc tccgccttta tcgcactgtg ctcggccgta ttcggaaagc
    38341 cgattcagtc ccagatggtt gtcctcggca gtatgagtct tggtggaaat gtaattccgg
    38401 ttgaaaacct ggctgagtct cttcaggttg cctttgattc tggagccaag cgcatcctgc
    38461 tccccatggc cagtgtgcgt gatatcccaa ccatacctgg cgagctattc gccaaatttc
    38521 agacaagctt ctactcagac cctgtcgatg ctgtttttaa ggcactgggc gtcgaataag
    38581 acatgcttga gactattggt gctctgattg gcttctgatg agttatgaga tctgtatgaa
    38641 tttaaatgaa atcatcgatt cactgcggac cgaggatttg gtcagccagt ttaagagggc
    38701 gctcaaagat gctggcgttg agatagcgga agatcccgta gcgaaatact ttgcggcgga
    38761 gacagtggat gccacattac ttgtgctggc tcatgagtca actcttgaaa agggttggtg
    38821 ggggattcac tcagacatcg ttgagcgggc taaaaactct gaacgggtga aaatgaatca
    38881 tgttggatgg ggagccgtcc ttcttcataa gtcttttcag cgtgggtatt ggatccgagg
    38941 cgatgacata ttccgaatga agaccctttg cagagtgacg ctcggtaaag aaggacagta
    39001 tcatttcaac ggggatagtc ttgaaaactc gccagagttt gctaaccaat tttttagcgt
    39061 taagactttc ttgaagctca gcgggctaca aaaaagctaa tcggcatacg tttgcaacca
    39121 taaaggctag aaggttggcg cacggagggc ttcgtatggc tggcattcga tcgtttcctt
    39181 cacccagtat tttgggcaga gcccacaata tctatattga tgacctttgg aagagcgatg
    39241 gttggggttg tacgcagtgg agaggttaca gcgcccagat gttattctgg tacggggttg
    39301 caggtgcgcc ctatgctggg gggtccgact tagcagacag gtatctcgcc tctatcattg
    39361 gcaagaggaa gatcagcggc gttcctctgg ccaagctgaa attgtggaaa tcagtaaacc
    39421 gacttgtaaa tgacattcgt cgagagactg ggatggagct tgtcccatgg ttggcgcaaa
    39481 ctcgcatcga cagcgcgtgg gaacgacttc aacaggttta tctcattggg cccaagattg
    39541 cctcttggtt tctacgagat atcagtttcc tcatggatta tcgcatcggt ggaggaccgt
    39601 cacggttttc ctatggatag cagcgtaatt cccaatggtt tgctagtctc agccccgacg
    39661 tgatgaaata ttttgttccc atagatcgat gggtgtttat tggggctaag aaaattggag
    39721 cgctcaggcc gagatctatc cgtctcaaag ttcaagggct ccaggcaaac cgcagaaatc
    39781 acctcaaagc cgcctcagag atagtcgaat ttgcacaacg gcgcaatctt gatccccggg
    39841 acctgaacgc gtattggttc atggttggga gtaaatggat tgatggcaac ggaaaaccac
    39901 cacgtttatg aagaagcgaa acactatatg cgcgattgag ggctatggct aaactctgaa
    39961 cgagctcctt agatggcgcg atcatgacca tggaaatact ggcgctgatg cgtttcatgc
    40021 ctaaggctac ctgggaacga tcctttctct acccagacaa agaacactga atgacacggg
    40081 ctcaaaaact tttgtgcatt ctaatttatc tggacgccgg acagttccgg aagctttaag
    40141 gtctacgcag gatctagtga accgagaatt ttcaaaccga gaaaacgtcc catatttatg
    40201 aagcattcgc ttcccgatct tgtgaaagcc cagctccggc aggcggtggg aattccaatt
    40261 gatgccaatg ggtatgtatc tcgtgtaatc gacaatttgc tacctggggt aagactggat
    40321 cacttcgaat cggatcttaa gcgaggtggc ggccatgagt taaacaagaa gtctcttgca
    40381 ctccactcgt ccgccgctct tgctatcaac accttcgctc cattcaaaga ttttcccaga
    40441 tcgttgagca tacttggtaa gcaaggcttt cgccctcccg tgtttgagaa acagctgccg
    40501 actggcctaa gtggtacggc acccaatatc gacgtctatt tacagagcga tgatgaagtc
    40561 atcgccattg aatccaagtt tctcgaacat tggaccccaa agagggcaaa atactcaggc
    40621 agctactgta aagacagttt gccactagca gaggattgct ggtggcaggt cttagaggag
    40681 tccaaggcgg gtggcctcaa gcaccttgat gtggcacagc tagttaagca ctattttggg
    40741 ctaatccaac tcatgaagca ggtggaggga cggaaacata aagaggtcac acttctctat
    40801 ctctactggg aaccagcgaa tgcctgtgat atccaagatt gcctaggtca taggcggaat
    40861 atcggggaat tggcgtcaaa ggtaagtggt tcaagaataa agtttaagag tttgtcctat
    40921 cccgaactct ggagagagtg ggaaggcatg ccgggcattg ctgctcacgt gagaaatctc
    40981 aaagctcgat acgcactgac gctgaaattg gcatgaatcc ttctcctttc ggccaaaaca
    41041 tccgacgatt ccgtgcgtaa tcttagcagt acggtaacag gttgtaggag gctgttctca
    41101 gaatgaaggg gccaatgatg ggacgatcat attttgtgcg cgcaatgtca cagacagaga
    41161 aggagtttaa cgtttttttc gaaaatagcg tggtagctgt aggctggagc tcagtcgata
    41221 tgagtgaatc gacgaatatt gacaaaatcg tcgacgcagt cgattgtgcg tattatcgag
    41281 gcaaaccaac ggtgcctcag gtcgttggta gaaaactaaa cgaggttcgc aggtttacgg
    41341 gtttatccgc gggagaccaa atcctggtac catattattc ctcagtgagg ttggcagtag
    41401 tagaaaatgg tcaggtctat aactctagtc cagagctcga cactgatcac ctggacctgg
    41461 ccaatcagcg acaggtcagg tacctcaaaa tcggaaaaga ctttgtggag atacctagaa
    41521 ccaaccttag tcacggtctg cagagccgac tcagaatgcg gggaactaca ataggaagtt
    41581 tgacaggatt tgagaccgag attggtgaga tttttaacga tccgaaaacg acttggaagt
    41641 cccgtttcgc gatgagtgac agaaaaaatg ctcaggaatg gcaatccaac ctgctggcta
    41701 gtattcaaaa tggcaggaca aatttaaagg caggcggccg agggctcgag gaattagtaa
    41761 gagacatgct tacgcttgag ggctatcagg caaaaaccct atcaacgagg agatttccgg
    41821 ggatcgcaga tgccgacgtt gaagccgtca gagcagatct ctttgcccaa acgaagctct
    41881 tggtacaagt taagcaccac tacggtcaga gtgatgcatg gggagccaag cagttggatg
    41941 agatttttaa tgatccggag gcgtttgtag actatcaacc agtcttggtc actacggccg
    42001 attcttcgga acagctgacc gaatattgtg agagtaagca aatcagactt attacgggtc
    42061 gagaattagt agaaaggatt tacgatcagc tggaacgact ttcatcggac tggcgcgaaa
    42121 cacttggtat cgggaagacc ggacaactac ttttttaatt gccagcactt gtaactccga
    42181 ctatcaagac gaatcgaact atgcagtacc gagctttatt tgacgaaaca atttgttgct
    42241 gtgttcaatg ccgagtgtgt cctggactcg ctacctcagt tagagttctt gctcagcgca
    42301 taagtgtaaa ttccgtttgt gatgcacgga gaagagccaa gagcatgaaa gtgatcatgc
    42361 cccagaaggt aatccatctt cgataatcgt ggtcgatagg aatactccct ccgcccgttc
    42421 gataccttcg aaagattgcc catgcaattg tcccgaaaat caagccaaat ataccgtgag
    42481 atattattgc actaaatacc tctgcgattc tttcttcaaa ataagaccct attcctatag
    42541 tagaggcaag tttattatga gagcgggcgt agttgtactg gagccaggta aggagcagcc
    42601 catgcgttaa ccaggctgta actactgcca tcacaaatcg ccaagaggaa aacagtcgaa
    42661 actttacttg gggtggtaca gtggacgatt gcggtgcaag gtctccggta tgactcgtgg
    42721 gtccgtgtaa tatggtgtta tagatggact tcagtaccgt tgcatttgtc caataatcaa
    42781 catggcaagc gagaggatac cccagacccc acagatgcac attatcgatg gtcccgctga
    42841 taggacggac atgatggagt tttcccgaga cgatgtcgag tattgaccag aagttcgtcc
    42901 aagacataat tcgagggccg cgatcgtttg attcgggagg tggatttcga ttcgaggcgt
    42961 agtcaatgta ccagaatttg tcgaggggtg agccaatggt atagagagaa gtaatggtag
    43021 cttccggcag ggtctggcga ttgtgaagcc agtcatagac aatcactgag cccaaactat
    43081 gcgcaagaat atggatttgt gtgcatccta ctttctgaag agcctgataa cccgcttcaa
    43141 ctcggtccca cagcttcttc cgatcggaga tcgaggcaag gtggtaattg cttacgtcca
    43201 ggatcagatc aaagagcgtg ttaacccact tccactgcgc gaggaggact ataagggcta
    43261 gcccgatcaa taatgattca agggaggaaa taataagatc gagtgggatc gttttgtcca
    43321 aatcatcgcg ccatttctgg atgatgtccc ctagagggcc taataccggt gacagaatga
    43381 ccccagcgag gaggaaatac aaagcgaaga ccatgaaaat tatatttatc catattgccg
    43441 cgactagtcg atcgcacagt ttatggtcgc tcgacacgag cacaattaga ttgccaaatg
    43501 acttggccgc ttgaacgatg gattgaaccg gcatgacgac cgttacgtga gcaaatcgat
    43561 ggatccaaca aagaaattta ggagtggccc atccggttaa ggccgagcta agttgctgta
    43621 gggggtaaag gaatactacc cctactacat tagtgacaag agctagaata agcagggcta
    43681 cagtggcagg aactccgatt atgaatccga acgatgccag ggcagctcga aacatgccgg
    43741 catagagcgc tggtataaaa aagatgccgg cggtggccca atgggcagtg gtaggttcta
    43801 caaacgtctt tacccattgg cgcatcacga ataggatatt tgctccaatg aaaatccaga
    43861 agcaaatcaa gaggacctta gagttctcta attgtattac agttattttc agctgaggtg
    43921 gaagggatac ccaaagacgg cccgaaatca aaagaagctg tgtgacgagc catattcccg
    43981 cgacaatcga catcccgaca tacgagaaaa acgcaatggg gtttgaagtg gaaggaaccc
    44041 tttgatggaa caccaaataa ttccatccat gttttaagct ttggagtaca ttgataaagg
    44101 tgtccgactt aagatggagt atgggtaaat tatcgacatg actgagatcc gcccagtgtc
    44161 cttccgcaat gaggacggtt tgctcagata gttgaatgga aacaacgggt gagttctcgg
    44221 tcggctgaac taaagatgtt attgagttat ccagtcttgt tagttctgag gcaatagttc
    44281 ttgcaatagt accttcctgt tggtgcccta ttccgtggat gatcatgaga ccaatgtttg
    44341 gcataaatgt cttctcagtt agggtctgtg cattgcgtca aaaccctaaa aaagcgactc
    44401 tgggcataag atcatcctag atccataaaa atatcagatg gaatcgaggg gaactgagct
    44461 agctctgata ctatacaaac gtgcttcatg gagtagacga attagtacct aggtgcaaag
    44521 acacaaatta aatacaagca ttttgactca cctcactaac gaagattgcg ccactccctc
    44581 caaccattcc tcgcaacata gtcgggctgg ttgtgacgtc tcccccgaaa acgacggcag
    44641 tttaaagcga gagtagtatg gcagtctgct caccgcaaat aatccattgt tggcatgaac
    44701 taggtagaca gcaaaaaatg ctactcaagt acgggaaggc cattgcgcag atcctggaca
    44761 tacaaccacc caccgtccta tacaaaaaca acggagtgga gttgtttttg tagatcacat
    44821 tgccgttctt ctcaaagctt cacaactcca cgctgttcgc attgtaaggc agttacgtag
    44881 gtctcttccg cgtcctaaca aataacctat agcagaacat ctattgtggc tccagcatac
    44941 cgtcggccca aaattgtcct ttcgtgggtg gcgcggctgc agaaagagcg gaacctcctt
    45001 ctgcatatcc cggccgggag accttcaagc tttgcctagt ccaactccca acgatatcca
    45061 ccccaggcaa ggctcccaac caaattccgt agagaaattt gaaagaaatt gtcgactatg
    45121 gtgtagagct aattcatccg ttctggctga ttcttgaccc gactcgtcaa gaagttcgca
    45181 tccgaacaaa tacgtttgcc tcggaattat aagtggcgaa agactatcca tacgacatgg
    45241 tcttctgact tgaagaggta agacgggtca cctctttgct tctggattgc ttgtcccgcc
    45301 gtgtcctgca ttggaatcct gcgaattaca ttgaggtcgc tcaactcaaa tccggaagcg
    45361 caagaacata atcctggaca aaaagtgaaa ttttattctt tatagcctca cgcacacttt
    45421 ctagtacctc agaatctata gagaccttcg cgctgttgtg ccttagtcgc acttcattcg
    45481 atctaaattt aaagaacagg acttgtgtca aattggacgt cgcctcaaca ccaaaggcca
    45541 gaagcgaaac gagaaactgg tcgctatctc cttgctctac aagcattatc tggaaacgac
    45601 cgctgctcgc ggactgactc tcccggctga acagtcggat ccaggggctg tcggcactca
    45661 tatccttaag tgcgccgaga gtggcttgta gaaccgcaag ggctggggct gctggaccaa
    45721 gcaatgcagc agcgacttta agtactgcct catgagcagt aaaatcactg ctcgactcgt
    45781 tgtagtccgc gaaactgcgc tcctggataa cccatcctat attcgtaagc acattgaaat
    45841 attcgtcata ccacccatag atatcattcg ggttagcgat tcgttttttc gctacaagct
    45901 gagctaatag cgcgcttcga acgatatcgt tgcgccgctc gggcgttact cccttaacaa
    45961 acgatacgat atcagagccg actacagtag cctgattcct agcggaatcg aaatcgaata
    46021 ctcccgcact ttcaacggcc cctctcttag gagtaggtag atcaaccgaa ttaacaaact
    46081 gagcggcatc agacattaag ctttgtctca tatgcatccc tccaggttaa gtccatcggt
    46141 gcaggcgtgg aaaccaacca tccacaattg gctgaggcaa gtttcttgcc cccgctctag
    46201 ttcttaacca tcgattgcat cgacagggaa ttacagcgca aatcgaatgt gaacccaaga
    46261 agtaggcagg acgtagtatc tgattagata cctagcgtat ctaattagat acacaatcgt
    46321 cgtaataatg tagcagttta ggccgaaaaa tgtgtttctc gaatcgcatg acccttgcac
    46381 cattttcttt agaaggacat gaccccagga tactcatttt gatctttgta ttttcgcgat
    46441 tagaatttgt atctgtttaa aaagtgagac agtgattgta tgccgcaaga tttacaagaa
    46501 gtgaagagat taaccggctc tgaaatcgaa gcaattgttg aggcgattgt agacggttat
    46561 aaccgtgcgg ggctcgagca agcccttctc tttgactggg gtgaaattct cgaaaatcga
    46621 gtcaatgtct atactggtct cttcaatatc gccacggaac tgatcagatg gacagagctt
    46681 aaaggtagga ctctagatct tgtacgactc ctttatgaac gcaatagcgg caacccgaga
    46741 ctgagacagg tggcagaaag gtataggctc gcagcgtcta atgctccaag accaacaagt
    46801 cttgaatcac ttataagcac ccgttcccgg ttcttagact atgatcggtt cacttctcgt
    46861 ttcgcgacct ttggacgacg agtctgtaaa attactacgc agcacagaac tggtacgggc
    46921 actctaatag ggcccgatct agttcttacg aattttcatg tgattgaaga ggtctattac
    46981 gcaccatctc ttgcgactgc agtcaggtgc cacttttcgg gtattggcgg tgcagatgac
    47041 gagaatgacc tgcaatgcgg cctggctccc attccgaatt ggattgttca gcacagtcta
    47101 tgcagttcat ccgatattac aggcaccggc actgcaggca tggacgagct ggattatgct
    47161 ttgctacggc tagaaaaaaa tataggtgag gaaacatcac acgaaggaca tcgtcgcgga
    47221 tggttcaaac taggagagag cgatcccatc atgggcagag gagatttcat tgtgattccc
    47281 caacatccga atggttccag cttacagata gcatggggac aactgttgga accgaactcg
    47341 accggatcgc ggtttcgcca tgatgccacc acagatggtg ggtcatcagg agcgttgtgt
    47401 ttcaacaccg aacttgagcc attcgggctt catcatgcaa ctcaaccagt gatggttggg
    47461 actaaccctc actataatca gtgtattccc ctcaggcttg ttgccgctga tcttaataaa
    47521 catcaggata agacacatgg ctgatccaga atcagggcaa gtagatccaa aatactggga
    47581 aggaattatt agccagctgg tgcaatgttc tccttccgac ctgttggaca agatccttct
    47641 gaagtggagg tctaccaaat tggaaagtct ggtcggaatt acagggcgac ctcccaaaga
    47701 ctggatctat gagttgttca ctcaagctaa caagcaatca tggctttcgc ttttacttat
    47761 cagagttcaa tcatggactc agcaccctgg gcttagaact gagatcggaa tagccttagg
    47821 agattttggc tcagctcgcc ctggatggta cgatgttcca gtagcaattg ggcgggcagt
    47881 cattgacagg agtcaatttc gctcgacgtt gtttgaattt ttggaaagag attcagctaa
    47941 tattaggctt cttgttgtcc gtggcgataa tcctgggaag agtcattgta gatggttagc
    48001 gcagtacgtt gctaaccaga ttggaatgac cactttatac atagatttac tacaggatgc
    48061 ttctcgcctg agatcagact tagccaatag actttctctg cactacgcgg agtttatgga
    48121 gggttttacg acttcagctc gggactctaa agagtttgtt ggatggcttg caggaaaact
    48181 taataatgct cccctgctta actctcgata tttactcatt tttgaccata tagccaaacc
    48241 aggtgtcccg gttgaagcaa gagaagtggt cattgagctt gctcgtaaag cagtaaacca
    48301 agagcttacc aatatctggg tcattcttat tgacactgaa ggcctaggag aaatagagca
    48361 tcccggcgcg tccagcgaag aactggtccc gcaggtgacg cgtcacgagg tgaataattt
    48421 tctggaattt gcgtggaggt ttagaggagg aattggccat acgccccgga acgttcccgt
    48481 ggccttaaca gaaatgttag atggctcaat tgttgcttta tcgaaagaaa atttgaagaa
    48541 ggccgagcgt gaaatcgctc aatggatgcg taatattact tagcttaaat atctgtacgg
    48601 cgatgaaaaa taaaggcaat ttcaagaaac tttccattga attgtcagca acggcagacc
    48661 ttttggcgga ttggaaagag gtaagtccca atcaagaaat ttcatgggcg taccgcttgg
    48721 ctgcggcagt catggatacc tttaacccag gcactatcca gccttatgac gagaatggtt
    48781 caattcttag tcaaagttat ccaaatcctg agaatgatgc cgatcttgta attcctcaga
    48841 ttccggagag atggcggctt cattgtgacc tgcggcggga agctctggaa aaacttgcga
    48901 cccgccaaaa catgaaagat gccttagcgc ttaatcccga cattgcagac acttctctac
    48961 agcgcatgct gacatcggcg attaacgata caattcctga tgatctaaga gccttgaccc
    49021 gcgatgatct ctctgcatta gtcactgtgg cgagttggct aaagtccatt ataaaaatac
    49081 cggaccttga ttcggtagca agggtcttcg cgctgaagga tctactgtcg ccatttgagc
    49141 atctcacatc cgatggtttc gtcggtcgtg agatggaaat cacgcttctt catagctatc
    49201 tgacgtcaga tcaggatgga acggagcctt tactgttgta tggtgttggc ggcgtcggta
    49261 agtcttccct cttagcccaa tttatactcc aagccgttcg agatcccaca aatactccct
    49321 ttcttgtgca cttagacatg gacagcccca ccgtttcccc acagcacctg cacacttttg
    49381 taattgaaat tactcggcag ttgctggccc aagaccctgg acttagtgcc catgggagag
    49441 cgaaccttcg ctcagtccgt gatctcgcca aacggctccc ttccgacctt gaacgtcgct
    49501 atagtgaatc tactgggtca ttgctactgg aatacctttt gaacagtgcg agcgctctcc
    49561 taacaaatgc gctggagtct tccaaagaga ggaaggtgtt gatagttttg gataccttcg
    49621 aagaggcaca gatttttggt gagtctgtcg catatagcat tttaatgttt atgaagcttc
    49681 ttcaatctcg agtgcacaat gtgaggattg ttatagccgg gcggggaact ccaccggagg
    49741 gtttacgaaa acgtgttggt ctagagcgcc acccagttca aataaatagt cttgacccgc
    49801 gcagtgcagg cattttgacg tatcacctcc tagagaaaag ccatcttcaa catgatgaag
    49861 cagttattca ggaaattgtg tctgtagttg gcaggaatcc tctaagtctt aaactggcag
    49921 ttcgagccat atcccttcag ggatttagtg cattcttaga tacggactca cgcagtcaaa
    49981 tcgtcgaaaa gcttcggcag gagagagtgc aaggatatct cttcggtcgt atcctcaact
    50041 atctcaaaga tgagcggctc aaaaagctgg tataccccgg aattcttcta cgcagggtaa
    50101 cccctcagct gatctgggag gttcttaaag agccatgtga gatcgaagtt cagagtatag
    50161 atgactcgac tgaacttttc aggagctttg agaaagtaat gggcgttgta gagccggatg
    50221 gaaatgaagg attgcgtttt cgcctcgaat tacgtcgtga gatacttggt gacgttttgc
    50281 gtgtagtgga tccaaaaatc gctgaagcaa tagatgcccg agcggtgaaa ttttatgaat
    50341 cggataccac tccttttgga agagcagaag aactgtatca ccgcctccgg ttaaaccaat
    50401 ctaacgatat acttgaggct agatggtctc agaatgcgtg gtcgcaccta aaacaatcga
    50461 tcacggaatt accggctcaa agccaactat ggctcgccca aaaagcgaat gtaagtctgg
    50521 acgtggccgt gagaaggaag gcgtctgatg acgaatggga agacgaggcc gctcgaatgg
    50581 ctgaatcctt gttaaaactg ggtcgtgcag atgaaacctt agaggtgctt cgtgaacggc
    50641 cggctcgacc acgaagtagc aaaatttcat tactcgaagc tcaagctctt cgcttgctcg
    50701 gtagggagga cgaggctagt gagatattaa tattgggact taaggaagcc tcaatccctc
    50761 agaaagttga aatcctactc ctcttggctc tgatagacga aaacagagat cagctgcatc
    50821 gagcattaca gacggcgtcc caagcattca gaatcagcag caaaaccagt gatatcctcc
    50881 taaagctgcg aacgcttgta acccgacttc gtttgtttcg aaagcttaac atgagtagcg
    50941 agcatgagaa actccgtgcc aagatggtga aatctgtaag cgaaaaagta ttgaagcttt
    51001 taagggatca tcctgctctt tttcaggagg tagcagcaga acttggagca agtagtcaga
    51061 agatcctaaa agcagctatc gataggttag gcatgctaat aacgagtaag tcccaacgag
    51121 aagcactgat acgcgcttta gatcaattga ccacttctgt ctcggtgagt aacccatcac
    51181 gaaaacacga tgaattggga atcagtgatg ttgacgaaat tttacgaagt ggtggggcag
    51241 gattgggcga taaggttgta ggtatcttgg gcggaaagcc agtcgatcat actgttcttg
    51301 agtccttgac agaatatttt agaggtagtg tcgatgtgtc tcttagtcag aactggtcgg
    51361 aggggttatt cggaaaaggc aagcgtggag agaatgccta aaactagacc atatcttatc
    51421 gcgagattgg tgcaagccgg atggctacgt cttcattatc aataaagacc acccataagt
    51481 ccggacgaat cggatcatcg gtgggagtct gcccgcgtga acacttcgga aaattgagaa
    51541 aaaaagatgc gaaaatggcg tcgactatga tcgagagaat ggatgcacac ctaacctatc
    51601 agtgttattg atgctccgat tctctccgat ttggacacct ggttcctggt tggaacaaaa
    51661 tcaattgatc tattgccgaa tcccagcgtg tcctatatcg gacagcctcg ttgtaagcca
    51721 ttgtaaaacc gtgtattgga ttggaatgtt tcaccccata aggcgttgaa tttacttgtt
    51781 ccctggggcc cttttaaggc gagggtcctg ggttcgaatc ccagccgact caccacctaa
    51841 aatcaaagac ttacgagacg ttcaggcctc gaaaatcttc agaaagcgcc ccttgtgcta
    51901 aatttgtgct aaaccgctcc ggttggcact catccagcac cttcacaccc tctctcaggc
    51961 tttccggcga atgatgcgca tagcgttgcg tcatcaaatt cgtcttatgt ccgagcagtc
    52021 gttggacctt gtagagatcc accccgcgct gaacgagtcg cgtggcaaag gtgtgccgca
    52081 tgtcgtgaaa ccggaagtct ggaatcccag cccgattgcg agcctcacaa aactcccgca
    52141 ccagaaagcg tacctgcagg agattgccga ggggagtctt gaatactggc ccacgtgaga
    52201 ggcctgttgc ggcctgcttc gcggctagga gttcatacac cctggtattc aaggggatgg
    52261 tccggcgcgt gccgttcttg ctctgcatga caatcaaggt accgcgagaa aaatccactt
    52321 cctgccattg cagtttgaga atttcccctt ggcgcatgcc cgtattgagg gcaaagacca
    52381 tgatctctcg gagccaggga gatgaagcag ccatgagccg ttcttcttcc tcagcggtta
    52441 gccaccgatc gacctcgttc cgtacctgtt ccatagagac gcggtgcatg gggttttccc
    52501 ggcaccattc ccattcccgc atggcgaggt tgaaggcatg ccggacaagc tggagctctt
    52561 tattgatggt ggccggagcg gcttgctccg ttcgtcgttg tgccttgtac tgtgccatca
    52621 cttttggtgt gatatgcgcg agaaccttct ctcccagcac ggggaggagg tgccccaagg
    52681 ctgcttggtc acgtaaacgg cttttgatcg cttttagggt tgcccgctcc gtgacgtacc
    52741 gttccatcat ctccttaaac gtgcgctcct gctcctcacg tcgttcgaaa aactgacctt
    52801 cagcaatttg gaccttcacc ttggcataaa tggcttcggc cagctgcttt ttcgtggtgc
    52861 ccgtacttcg ccgaacttgt tgcccgtgaa acatgaaact catccaccac acgttcttac
    52921 gttttgtcag ccccatctgc atcctccttc tggatgaggc ctgatttcgg tctggtttcc
    52981 ccgtggcggg gagtatagac ctcgcgtttg gccgcttcaa tgacgtggtc aatgtcgcaa
    53041 ctgttgtggc gaatggggaa gggcagaggc ttttgtggaa tgttattgca ggtgtccagc
    53101 caggtctgga tagcctcgcg ctcgaaccgg acaaggccat acatacgtcg gcaagggatt
    53161 ttgcctttcg ccgcccagct atagagggtg ccaggtttga tattgagcca gtcggaaact
    53221 tcttggaccg tgagcatggt tcaactgacc gggggagtct tgcgacaccc ccagaccccc
    53281 tacagtggac tgcccgcgtt gccgccggca ggtgttgaga cagaccgttt gggtttcttt
    53341 ttgagcaggt tccggtgtcg gtctttccac cgggacacgc cccgcacaat ttcatcttgc
    53401 aaccaggctt ccccgccagg tgtcgcgcag aggacagcga gcatcggcgt caatgatccg
    53461 ctgacccagt ttttgactcc tgtgagggtc tgttcctgtt tttccaatac taagcgagct
    53521 ctctgaaatc cttccgtcag gacggcgtac cattcgacga gtggggctcg gcagcggtac
    53581 tcatcgtcat cctcccgcgt cgtctgccga aaatccacat gggaccggag gagtcccacg
    53641 ataaattctc gccagagcga ttcttcgaag taggcaaggg cattcccgca gatcttggcc
    53701 cgtgttttct tgaattcgag ttcccagcgc accccatagt cctgccagtt ctctcgttcg
    53761 ttgctctgca gttcgaggcg cttgtcgtag atgcgtaaca gggtctcgct ctgagggctt
    53821 cccacataga tcgtttcccc tgtggtggcg tagctgtcat gtatgcgatt cgacacaaat
    53881 cgtcgaacct ctttagcgcg agtcacacat tgtccgactt ccaaggcctg agcaatcgtc
    53941 gctaagggga ccgttcctgc tcgatcatcc agggcacaat caattcgcgt gagatgccct
    54001 tgcttctcga ttacccacaa gaggagtgaa cggacctctt ggagcgtgta ggcggacaca
    54061 atgccgcctg aaagatcgac gtgaatttca ttggggcggc ggggagcatt ggtgcctagt
    54121 ttgccgacac cccgcaggcc atcgaccctg atccaggaga ggggataccc gcgaaacccg
    54181 gctttggctt tgttccactc tccaccaagc gtgcgcatgg tctcttctgg attgctggcg
    54241 ggaacagtaa acgcgagcca atcaatggag agcgtgaagg gagaatccat agaatctatc
    54301 gacctcctag aatgagcgct ctaagtagcg cccccgtgtt accacgacgg gggcgattcc
    54361 gccccgcccc gcagcctgtc ggcatgcggc gcggagcggc tacgtctgtg gtgatcggag
    54421 ggttgccttg tacgctggtc atgatggtta ccgtttcgct ttccgatctt cccgtgtccc
    54481 cgccggtttc tggattttct ttgcaggctt ttctgtgttc cccttcgtcg cctcctcgct
    54541 ttcaaattct ttgaggtatt tttgggagag ctgagcaaag gtgacccctg ggccgatttg
    54601 ttcccactct gcctcgccgt cctcacgaaa gccgagcggc ttttctccga cattcttcgt
    54661 tggaagatcc tcagctagct gatcaagtcc cgctgcaagg agatcaccga tcagatcggt
    54721 catcgttttg cgcggataca tctcattcag tgcggcaagc tgagcagcca cccgaatggg
    54781 taggcggact gtgtactgct tcgcggtgag tcgtgaatta tctggtgcat tccaaattgc
    54841 atgtaggtct cttgtgttca tgcgtcctcc attttgttaa cgtctcaata agacttttat
    54901 aaagtctaat acagacgtta atggatgtca acaaggggta tctcggcaag caaggtggat
    54961 caccattgcc gttgatttca cagatgcatt aggaactaaa tgtttgactc tcacccgatc
    55021 acgcggcaat attcggggcg atggcaggcg acgatccaca ccattttgat tttgatctcg
    55081 accgtggcat tcgatctcaa gttgttgaaa agcttgagtc aagcccactt tttccattga
    55141 agaagggcat tgggccgcag gcaagtggaa tctacgccct ttattttagg agcacgctgg
    55201 tttatatcgg caaggcgtcc aaggggacga ccaaaagcaa gcgaacatta cgcggacgcc
    55261 tcggtgagca tgtcggcaag atcagcgggc ggcgaaatat ttctctggct gatatggagt
    55321 gtcgttactt aacgtttgat agtgagtggt gggttttcgc ggctgaattt gcgcttatga
    55381 ctcactaccg acctgaatgg aatgaaagcg gatttggcag taaggcgccg ggagtgggac
    55441 ggcctggcac tcatcgtgta agtcgatgga acgagcagtt cccggtacgt ggttaggggc
    55501 acccgcgctc gcaatcatcg ctgttgcggc agatgatcca aaaacaatgt cgtttccgcg
    55561 catggtgggc cgttttccat ttcgtcgaaa ccattgggcc cttcctaact ttgatgatca
    55621 aatcgcaaac ggtaaatccg gcttcttctg ccattcgcat gaagtcgcaa tgtggccatt
    55681 tcgacttatg attgttcacc atgtcggtga tcttcgcaag tagcaagccg tttggtttta
    55741 atactctctt tgcttgcgtc aaaaatgctg gatacaaata gctcaagttc cagtcctgct
    55801 ctgcgccgca ctccattgtc gccccaaaat ccacatcgaa tcgctttcgg cttttatctc
    55861 gcccctgtgg tccaacatgc ggagggtcaa ataccactac gccaaacttc gaggaaggga
    55921 cacctttcat ttttcgattg tccccgacaa tcattggctt gtatcgaggg tcaatgtcca
    55981 tagatacgac atctcgtttt gagcctttcc agaatcttcc cgcgttatac gtggaatcta
    56041 aaattggctc tggggggatc gttgcgtaaa atgtaaatat cgcttctagt agatcgccgt
    56101 cgcttccctc ccatatgctg ctaatcggtg catatgttga tacaacgttt tcagcctccc
    56161 ttattttgct ctgacccgca gatttttctt tgggattgta tctaaggacg atatctgtga
    56221 accgatcaag cacggtggtc tcaaggggca tcagtttctc ctgcgcaatt aatgcttaat
    56281 gattgatcct gcttattcgg gacatggggc taggtgttta tcgtgcaccg ggcgaactca
    56341 cgcaagcgat agcggcgctt ttacctatag gtatgtgacg agcccgtgat ttgtgcctat
    56401 tgccctcttt gtcgccggtg taggtctctt caacttcgac gaggccgctg agcatcgtct
    56461 ggtcgctgtc ggaagcctca cggagccgtt gcaggacgaa ccatgccgac ttttgcgtga
    56521 taccaatttc tttagatagt tggagtgagg aaatcggctt gcgcactgtg accagaagat
    56581 acatagcgta aatccacttg tgaagcggca catggctgcg ctcaaaaatc gtgccggtac
    56641 ggaccgtgaa atcttccttg caggcgttgc aacggtagta accgcctttc cggctggtaa
    56701 tgcggtcgct gcccttgcat gccggacatg ttatgtcatt gggccagaga cgcttttcaa
    56761 gatacttgcg tccagaatcc gcatcaggaa acctcttaat taaggcgctg agtgtgacct
    56821 tattcattac ttgactccct tgtttgagcg cgtattattg gtcattccag ggagggagtc
    56881 aagtatataa tagactgaaa ttgatggcaa taccggagca cggaaagaag ttgagacgta
    56941 tggtgcatca tggtctgtag aaacggcggg tgtgctaaat ttgtgctaaa aaatcctgaa
    57001 agcctcacta ttgagtagaa ccagtagaat ccagggaaca cagctaaccg attaattcac
    57061 aagataaaac ggggaaacct tcgcaaaatc aagccgctat agaaatacta cttaacgaac
    57121 ttttaaggcg agggtcctgg gttcgaatcc cagccgactc accattcaaa ttcggcattc
    57181 ctgtaatccc cgctagtgct cctgaattca ttagcccgct gctttttctc cggcgtgagt
    57241 cccgctgatt cggcactcca tctgtgattc gaagtttccc cccggccgtc tgctgtctgt
    57301 cgcacattca gtcacccgcc tgagccgtgc gtttttaggg ttgaggatgc tccggtagac
    57361 gtgcaagata tcgattcacg ttcttatagt ttgctctgcc cggtaactct cagtgccgat
    57421 cctcagagac atcacaacca tggtgctccc aacgtgggta tcacacggcg tggccagtgg
    57481 cggccgcgca cacgaaagga atgacacgtg atgacgagac gactttcgaa gcagtggcag
    57541 gtcaggggct ggacttgtgc ggtactgctg gcggcgacgt tcaccaccgg ctgtatgcat
    57601 catcccggtg gcatcgcccc ttcgaccaag ccgctggccc ctggcggata taccgaactc
    57661 ggcaaggtgc gcgggcagga ttgtgtctat cacctgctgg gcctcattcc ggtgacgggt
    57721 ggcaatgaga tgagaaatgc ggtcgaggat gcgcttcgca cgaaaccctt ggcggatgcg
    57781 atggtcgagg tcacggttga cggctatttc cagtatttca ttctgttctc gcgagcctgc
    57841 acccaggtgt acggcacggc cgtcgaaacc aaatagccgc acttgtgtcg tactcgaatt
    57901 cgacagcagg atccggagtg ttgcgcgggc ttctgcctgt taccctgtag tgactcatgc
    57961 caaggtgaac ggagtgccta tgacctcctc ggcctcgact acacggcgga tcaaccggaa
    58021 catcaggcag gacctgcgtt gggcggccat cctgacccgc gatcctgccg cggacggcac
    58081 cttctactat tccgtcagga cgacaggcgt gtattgccgt ccttcctgtg cggcgcgtca
    58141 gcctctgcgg gagaatgtcc ggtttcacgc gacctgcgcg gatgccgaac aggccgggtt
    58201 ccgtccgtgc aagcgttgcc ggcctgatca gccctcgtcg gttgatcggc aagcgggaat
    58261 ggtggtcgcc atatgccggt atatcgagca ggccgagcac ctgccgtctc tcgacgagtt
    58321 ggcgcaacgc gcgaaaatga gtccgtccca ttttcatcgg atctttaccc acgtcacggg
    58381 cctgacgcca cggggatatg ccgttgcgca ccgcgcgacg cggttacggt ttgaattggg
    58441 gcgtgatcgt ccggtaacgg aggcgctcta ccaggccgga ttcaattctt ccgggcgctt
    58501 ctatgaagcc tcgaatgccc tcctgggtat gaccccgtca cgctaccggg ccgggggtgc
    58561 acagaccgag atccggtttg ctgtgggaca gtgttcattg ggggcgatcc tggtggctca
    58621 aagtgagcgt ggggtctgcg ccattctgct gggcgatgat ccggatgcgt tgacgcggga
    58681 tcttcaggac cggtttcccc tggcgaatct caccggtggg gatgctgcat ttgagcggct
    58741 ggtctcaact gtggtcggct tcgtggaagc tccggggcga gggttgaagc ttccgctcga
    58801 tattcggggc acggcgtttc agcagctggt gtggcaggcg ttgcaacgga ttcccgtggg
    58861 ttcgacggcg acgtataccg agatcgcgac tcgcatcggt tcgcccaagt ccgtgcgagc
    58921 ggtcgggcgg gcttgcgggg cgaatccgtt ggcggtcgcc attccctgtc atcgagtcgt
    58981 gcgcaacgat ggtggcctct cgggctatcg ctggggggtt gagcgcaaac ggatgttact
    59041 tgaacgtgag tcgcgtgtgg ccccgtgaag cgtaaaacgt aaaacgtgaa aggtgaaacg
    59101 tacctctgcg gacgagagac cagcgacgtt tcacgaacgg cggatcgtga ggacagcctc
    59161 tttgcgccct caaggtcatc tccgcgggga atgatggtgc ttttgcaccg ctggtggtgg
    59221 ggttccacca cccgatttca tcgatccccg gatgtccggc tatccatcca aggcgaacca
    59281 ggcgatttgg cgtgaccctg ttcaccccac gctgcaggcg tcctccttgc attctttgca
    59341 cgattgaatg gtgtgagaaa acccgttgtg aagggcgagg ggaggtcacc atgggaatgg
    59401 catggccgtt cggattggtt gcagacgaga aggtctgtct agggcgggta ctggtggtgg
    59461 acgatgaaga ttccattcgc agcctgctac gcatgacgct ggctcaggcg ggatacgacg
    59521 tcgaggaggc ggaggacggc ggcagggcgg ttgaggtctt gaacagtggc gacaatccgt
    59581 tgatggtcga tgtcatcatt tgtgatatcc ggatgccgcg tatcaacggg gtcgaggcca
    59641 ttgcctattt ccgcgcccaa tatccttccc tcccagtcat cgtgttgacg gggtatcacg
    59701 atgagcagtt ggccagttcg ctacgcaagc aaggtgtggt ggagtgtttg gagaaaccgg
    59761 cggaacggga gcagattctc acgtctgtgg cgcaggctat tgccggtcga cggatcagtt
    59821 tttacccgtg aggtacggtc tggcgagggg gagggtcccg ttgcggctcg ccaggtctgt
    59881 ccggttcacg tccttcctgt ttgattttcc accggcagct tcttagaatc ctccgagcgc
    59941 acacacatcg cttgatcagg aggaccagca tccatgtgtc acttcatcgt ccgatcgtct
    60001 cctcacatcc cgctccgtca cctcgtggct gcaagtctgg tcggcctgct gttcatcggt
    60061 ctggcccctt cccccctcaa tgccgcccgc ggtggcggca agaccgaggt gttgccggtg
    60121 caaaaagccg cgccggcggc ggtgatcatt gaggggctcg gcaatgaagc ggagttgcga
    60181 cgtcagatgc gggccaagaa cggggtggct gaactcgggg cttcgaccgc gcaagtgatg
    60241 ttttcgcaag cgccgacggg gacgtttgga ttcattgtgc cgaagctcct aggcctggcc
    60301 ttggtgacgc agagcccgga tctcgccgtc gctcgggtgg cacccgccga aaatgcctac
    60361 gagattcaca agcttgccga tggcagtgga atgttggtcg gattcatggg cgcggaactg
    60421 atgactcagg tggcgccgag ccaacgcccg aagaatgtcc gtatcgcgct ctattctaat
    60481 ccgtcggaaa aggcgccaca tatcgtggct gtgccgctgg tcaagctggt agcagaccgg
    60541 atgccgatcc ggctcgacgc aaagaatccg aatggtcctg tcttgctgga tatggatcta
    60601 cagggcagct cgacgcgtgc gtcgacccag ggtgggcagt agacagctag gctattggcg
    60661 gggaggcggc atcgttgcgg cctccctgat cgcttctctc gtgacgcgcc tcggtcctac
    60721 tctcccacct tcacggtaat gctatctgta gcggttccgg cgtccttatg atcgtggtcg
    60781 gcgatcttca ccgtaatcgt atgctccccc ttggccacgc tggtaaacgt gcccttgaag
    60841 cccttctggt acgcgccgtc gaggtagacg tgcgcgtgat tgcctgccgg cgcgttcagg
    60901 atttcatact tgatgtcgaa ggtctccgag accttctcgc cattgcgcgg ggagaggata
    60961 ttgatggtag tcgtattttc agcggcacac caggaattca gggcgagggt ggcgccgatg
    61021 gtcagtgcac aggcggagag aaccaatcgc ttcatggaaa cctcctcaga atgatgatga
    61081 cgaagaatga gtcgtgccga atcgtcgatg ctgcgggtca ggccctgcct gattgtggca
    61141 tcgctgccgc aaggtctgtt gccagcttgc ggaaagtaac gggcagaggc tcaggtgttc
    61201 ggccgcggcc aggttgttcc gttcatggtg catgatccga ttggcggtcg cgaccgtcca
    61261 ttcagggcag gggcgttcca tgagctgcag gggcgcgccc ggttcgaccg taccttcctg
    61321 gatgacccgg aaataccagc ctgtgaagcc ggtgcgttcc atttgtaccg ccagatcttt
    61381 gatctgccag cgacgggcga gtttccagca gggctgtctg ggttgagaaa tctgcacgac
    61441 ggccgtaccc gctgaaaaga catcgccgat gcagacatcg gcttccgtga gcccctccag
    61501 cgtgaaattc tcgccgaatg cgccgccggt gagacgatgg ggcggcagga tcccgcgcca
    61561 atacaaccag tgctccgacg gataggcaca gaccgccttg tcgacgcctc cgtgatggac
    61621 gagatccgcc tgtccatctc ctgccagatt gtgccggcca agccggatct ggcccggggt
    61681 agagcgcttg aacatcccgg tcgtccaggg cgttgagtcc ggatcgtccg cacagtcgct
    61741 gtgctcactg gacgggagcc ccacctggat cgatcgtatg agcccgcttg tgttctgcac
    61801 agccccgtcc cttccgccac gagttttccg cttttcccgg ccgcagtcaa cctagtgccc
    61861 gggacgagaa ggcgtcaaag cgtttgttct gattagtatc atcacttcca tttatgataa
    61921 aacgcggcat ggaatttcgc catctccggt attttctcgc cgtggccgat gcgctgcatt
    61981 tcaccaaggc cgccgaaagg ctgccggttt cccagccggc gttgtccgcg cagatcaagc
    62041 agctggagca ggaagtgggg gtgccgctgt tcgaccgcgt ggggcgatcg gttcaattga
    62101 cgcgggccgg atcgattttt cgcgagcatg cccggcgggc cttgcgggag atggaactgg
    62161 cgcaagtggc gattgccgag gaagaggggc tccagcgggg gaccctcacg gtcggtgtcg
    62221 ttcaaaccgt gaacgcctat ctgattcccg aaatcgtcgg ccgcttctcg accctgcatc
    62281 cgctggtgtc gctcaagctc gacgaactgt ccggtccgga catcgaggcg ggcgtcaaga
    62341 gcggatcgct ggaagtcgct atcgggttcg tgccggtggt gtcggaccgc atcgagagcc
    62401 agccgctgtt tgaagaagac tttgtgttga tcgcctcccc caggcaccgg tttgccgggc
    62461 gccggcacct gtccctgtcg gccctggcgc aggagtcgtt gattctgctg ccgaatatgt
    62521 tctgcacccg acgcttgttg gatgggtgtt tcgagcaggc cggtgtccag cctaaggtta
    62581 tcgtggaaat gaattccatc gaggggattc tggcgactgt tcgcaggagc aacctcgcca
    62641 cggtcttgcc gcgcttgtct ctcgggctgg cgcgtaacca gacgctccga gccattacgc
    62701 tgaagaaccc gacacctcgt cgaggcatcg ggctcttatg gaaaaaggga ggctacagga
    62761 gcggggcggc caaggcgctg accgatcagg tgcgggctgt cgtgggtgag cattggcggt
    62821 catgagcgcg ttcgccggtt cctctgactc gcgggccttg tcatagtcgg cggggagtcg
    62881 gcttcaggcc gcctggaatc agcctcccga tacggactgc ctaccatgtg cctgtatcac
    62941 ctgttcatgt gcgtggacac gtggcccccg ggtcttgccg gcaggggcgt cgaggcgcaa
    63001 tatctgtggt cccgcggtca gatagggtat gatagcgggg ggaaatcagc cgacatgaaa
    63061 cgtctcacat gagaagggct catgtctgtg cgcgacgatg gaacgacgac ctggctgttc
    63121 gcgagcgtgg gcggtgagca ggtgacgcag caggcgcagg tgaccaaggg acggaaggaa
    63181 tagcggcgat ggctgaatcc gtgcgatttg caaccctggt gaagcgatgg atgatcgggc
    63241 tgtgcgcggc ctgcctgctg gcggtggggg tctatgtgtt gatgacccga tccggtgaag
    63301 cgcagccacg ccaggcggag aagcggcccg ccatgggggc ccggagcacg ccggtcgtgg
    63361 cggtggcagc caaaaccagc gaaatcaata tctacctgaa cgggctgggt tcggtgatcc
    63421 cgatgaacac cgtcacggtg aagagccggg tggacggcca gttgatgcgg gtgctcttca
    63481 aggaagggca gctcgtgcat agcggcgaat tgctggcgca gatcgatccg cggccgttcg
    63541 aggtgcagct gattcaggcc gaaggacaaa tggcacgcga tcaggcgcaa atgaaaaatg
    63601 cgcagctgga tctggagcgc taccgggatc tctacaaaca aaacttcatt cccaagcagc
    63661 agctcgacac gcaggaagcg ctggtgcggc agtacgaggg catcgtcaag gccgatcagg
    63721 gccagatcga caatgccaag ctgcaattga cctactccag cattaccgcg ccgatcaacg
    63781 ggcgggtggg gttgcgcctg gtggatgccg gcaatatcgt ccatgccaac gatcccaacg
    63841 ggctgttggt gatcacgcaa ttgcagccca tcacggtcgt gttcgccctg gcggaggacc
    63901 atttgccgtc tgtcttcgag cggctcaagg ccgggaagca gctggtcgtg gaggccttcg
    63961 accgggagca aaagcgaaag ttggccacgg gaaccctgct caccgtcgat aatcagatcg
    64021 atccgaccac cggcaccgtc cgcctgaaag cggtgtttcc gaacgatgac agctcgctgt
    64081 ttcccaatca gtttgtgaat gcccggttgc tcctggatat caagcagggt gtcacggtgg
    64141 tgccgtctgc agccgttcag cgcgggccga agggcacgtt tgtctatgtg gtgaacgagg
    64201 cggagcaaac ggtcagcgtg cgagccgtga cggtgggcgt cgcgcaggga gaagaagccg
    64261 ccatcgacac gggtttgacc tcaggcgagg tggtggtcgt ggacgggacc gaaaagttgc
    64321 gcgaaggcag caaggtcgag ctgcgtccgg cagccggagc ggtcccagcc agtcaggagg
    64381 cgccggcgcc cggcggtgag gggcgcacac gagggaagcg gacgtgaacc cgtcgcggct
    64441 gttcatcgtc cggcctgtgg cgacggtcct gacgatgatc gccattcttc tggccggggc
    64501 gctggcctac cgccagttgc ctgtctccgc ccttccgcaa gtcgattacc cgaccattca
    64561 ggtgatgacg ttttatcccg gcgccggacc ggatgtcatg acctcgtcga tcaccgcgcc
    64621 gctggaacgg cagttcgggc aaatgccagg gctgaaccag atgacctcca cgagctcagg
    64681 cggcagttcg gtcgtcaccc tgcaattcag cctcgatatc gatctggatg tggccgagca
    64741 ggaagtccag gcagcgatga atgcggcggc gaccttcctg ccgcgcgatc tgccggctcc
    64801 cccggtctac agcaaggtca acccggccga tgcgccgatc ctcaccctgg ccttgacctc
    64861 ggccacgctg ccccttcctc aagtgcgtga ttttgcggaa acccggttcg cgcaaaagat
    64921 ttcacaattg tccggcgtcg gcctggtgag catcagcggc ggacagcggc ctgccgtgcg
    64981 cattcaagcc aatccccgcg ccttggctgc ctatggattg acgttggagg atcttcgaac
    65041 cgtcgtggcc gccgcgaacg tcaatcaagc caaaggcgga ttcgacggac cccggcgcgc
    65101 gtccatcatc aacgccaccg accaactgtt tgccggcaaa gattacaaag cgttgatcgt
    65161 cgcctatcgc aacggagctc cggtccggtt atccgaggtc gccgaggtga tcgacgatgt
    65221 cgagaatacc aagcaggcgg catggatgaa tgagacaccg gctgtgatcg tcaacatcca
    65281 gcgccagccg gggacgaatg tcatcgaagt cgtcgatcgg gttaagcggc tgttgccgca
    65341 attgcaaggg accttgcctt cctccgtgca ggtctcggtg ttgaccgacc gcaccacctc
    65401 gatc
//
LOCUS       DAORQN010000002        51410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_40_length_51410_cov_26.717963, whole genome shotgun sequence.
ACCESSION   DAORQN010000002 DAORQN010000000
VERSION     DAORQN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 51410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51410
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40_length_51410_cov_26.717963"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PK614_00310"
     CDS             complement(<1..513)
                     /locus_tag="PK614_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRB80689.1"
                     /translation="MTDPINQSPANTAPRTGPSRTLVVIAAAAILGVMFLVVWLQSSK
                     YEPLVVGKEAPDFQLPDLNEKQLRLSDYRGKVVFLNFWATWCKPCREEMPSMEVLYKN
                     FEKDGLVILAVSIDRVTTKKEIPPFVKGLNLTFPVLVDSWGQTDKRYKLMGVPETYII
                     DQQGVLREKVI"
     gene            complement(517..1431)
                     /gene="ccsB"
                     /locus_tag="PK614_00315"
     CDS             complement(517..1431)
                     /gene="ccsB"
                     /locus_tag="PK614_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRB80690.1"
                     /translation="MMRSLFLFDMTFWLYLAALGLYVAYLFAKRPAMQMVPAGHPIED
                     LEERDGAWATQLGQVATLVTVFGWVLNTLALTTRAFERMEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLEFRYRIKAVGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAVNRGSQSRFYAAFPDLETIDELGYKAIML
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGHKAA
                     IYAIFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            complement(1509..2945)
                     /locus_tag="PK614_00320"
     CDS             complement(1509..2945)
                     /locus_tag="PK614_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRB80691.1"
                     /translation="MNDKPDAASDVSASAPRTSGLGWEEFSREVVDFFASIKLAMFLF
                     IVLAMTATIGTVIQQGERPETYVQEYGEEAYRWFLRLGFTDVYHTWWFTSLLGLLCVN
                     SLTCFHKRFPSVWRSMRQDKVSVSLAFIQGMKQQTTLSLNQSRDAVAERLVKLFAEKG
                     YRVLAKSDPGEVTVYATKGIMGRVGAHVAHLSATVIVLGGLLGSYYGFQEFGVCLEGQ
                     TYHIPRGNFDLRVDKFWIDYHENGSVKSYNSTLTVIDQGTPTTTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEAARINIKEKGSDKIIATVDLEWNKEKAVDGLPLKMKMTDFVAD
                     FAFNSTEKKVFSKTAEHANPAIRLAVDERSSVQSTPWVFYHYPDLFEIKDSAYQFEFI
                     GYQPKKFTGLQIARNPGINMVWIGCTMLVVGMTLSSLIYHRRLWAKIINGESGVTLHL
                     GGTTHKSQIDFQKEFRKLTEKINAQAQS"
     gene            3344..4492
                     /gene="metK"
                     /locus_tag="PK614_00325"
     CDS             3344..4492
                     /gene="metK"
                     /locus_tag="PK614_00325"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRB80692.1"
                     /translation="MRHNYLFTSESVTEGHPDKIADQISDGILDAIIAQDKFSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYTDASWGFDSNTCSVLTSIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETTELMPMPIVLAHRLTKRLAEVRKKKILKWVRPD
                     GKSQVTVEYKDGKPCRVDTIVVSTQHSPDVTNKQIEKDLMEHVIRPSMPKGLYDPTSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPSKVDRSASYMA
                     RYIAKNIVAAGLASKCEVQLAYAIGVADPVSVLVDTKDTEKVAPESLDKLVRKHFPLT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRNEPEFTWEKTDKARALRKDAGL"
     gene            4599..5855
                     /gene="ahcY"
                     /locus_tag="PK614_00330"
     CDS             4599..5855
                     /gene="ahcY"
                     /locus_tag="PK614_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRB80693.1"
                     /translation="MDYDVKDMGLADQGKLKIEWAEATMPVLRLIRKRFEREQPFKGI
                     RATACLHVTTETANLMKTLKAGGADVRLCASNPLSTQDDVAAALVRHEGIPTFAVKGE
                     DNKTYYRHIESAIAHKPHLSMDDGADVVSHLHSKRKDLLRNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSTLVVAGYGWC
                     GRGIATRARGMGANVIVTEIDPLKGLEAVMDGFRVMPMDEAAPLGDFFVTVTGNLKVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLSKKKLAVRSGVDQYTLKNGRRVSLLG
                     EGRLVNLATAEGHPSSVMDMSFANQALGAEYIVKNYKKLEKKVYPVPADIDKEISRLK
                     LAGMGVSIDKLTKEQVKYLASWEMGT"
     gene            complement(6185..7819)
                     /locus_tag="PK614_00335"
     CDS             complement(6185..7819)
                     /locus_tag="PK614_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB80694.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRAKNGRIRVMGFPPIGIMSLSSVL
                     KRSGHECVMFDQAHPETPNEFIIEEIKRRKPALVGLSFLSTTSYPYAKILARQIRAAD
                     STVKLAFGGVFASLNAGLVKLQCPEIDFVCRGDGEQLLLDLLDRLEDPQEVGGVTWAK
                     DGKVINNPNRTMERHLDQWPFPDRESLELDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSAQHVVDELKYLEANGYGSVYFVDDHFLLQPKRIDAICK
                     GIIDAKLTIQWGIEGRVDSVAQHLFPAMAQAHCRTVMFGIESGSQKILDRLQKEQTLA
                     EVETAVRNAKKAGIEIVHGFFTVGNPDETVEDMQKTFDFASKLPLDTFGFNRLCVYRG
                     TPLWQEYIKRGLVSDAKDWYKYFKCSEIDPTCLPGAVINSVRQEGLKKLFIYKLTRYP
                     IQTFKLLRRFLRFMPFRDVAYLIIKPFLGQKKGATKAEVLSRAVEHAEMKDAAAQLTQ
                     LSDELLHSVLEESKAERQRIQQEAEGSRELPMVNSR"
     gene            complement(8121..8993)
                     /gene="sucD"
                     /locus_tag="PK614_00340"
     CDS             complement(8121..8993)
                     /gene="sucD"
                     /locus_tag="PK614_00340"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRB80695.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKMVAGVTPGKA
                     GQEVEGIPVFNTVADAVRKTDCDTSLIFVPPPFCADAILEAADAGVKLIICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKRGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAVVMIGEIGGDAEEKA
                     AEYIKKHVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTATEKMKALEAAGVRVVKN
                     PAEIGHAVKAALGR"
     gene            complement(9005..10183)
                     /gene="sucC"
                     /locus_tag="PK614_00345"
     CDS             complement(9005..10183)
                     /gene="sucC"
                     /locus_tag="PK614_00345"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRB80696.1"
                     /translation="MNVHEFQAKQLFAQFGVPVPRGKEITSPEAATAWASELNTPVFV
                     VKAQIHAGGRGKAGGVKITKEKGAVAGIVKELIGKTLITHQTGPKGRKVHRLLLEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPEKIIKEAVDPAVGFQGHNG
                     RNVAFALGLQTMEPAVINPFVQMLGNLYRLFMEKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGIFKHDDVQKMRDLNEEDPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKDTVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            complement(10269..11870)
                     /locus_tag="PK614_00350"
     CDS             complement(10269..11870)
                     /locus_tag="PK614_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRB80697.1"
                     /translation="MDIHALQQIVHQTRDARRKQTIPKFSPADRDQLIHKYHPDYRTS
                     AYRPIRFGPNANDKTVVELAALLEGDSSIAENIDLTPHYTTDVLVVGGGGAGCAAALH
                     AHGTGAKVILATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFLDTLKGGHMQNDHELL
                     KVMVEDGPSIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVLNQKIQLLEFCAAVELLSDDHSHCTGAIFKDLDNHRQVVVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRMGAKLDHIDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDANSETNVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAASRAGSMPQGKLTLQHLRRFRAEAKQHG
                     SSSGVISPMILPAYTRKEPERTANG"
     gene            complement(11940..12650)
                     /locus_tag="PK614_00355"
     CDS             complement(11940..12650)
                     /locus_tag="PK614_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB80698.1"
                     /translation="MAQEDTNVIDQPEVLRHKMVTIEISGKKYEVPEGITVIKALWHT
                     GQEVVRGAGCLGGFCGACATYYRTKDDPKVRTCLACQMAVEDGMSFTIMPPFPARKAL
                     YDIQSLKDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPQGLDIRIKEIQDGRFNDEWN
                     KVLAMNEQELADHCATVK"
     gene            complement(12735..14966)
                     /locus_tag="PK614_00360"
     CDS             complement(12735..14966)
                     /locus_tag="PK614_00360"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRB80699.1"
                     /translation="MTTKADKIIYTKTDEAPMFATYSLLPIINAFSKAAGVSVELRDI
                     SLAGRILAVFPEHLTPEQRQPDALSELGELAKKPEANIIKLPNISASLPQLQAAIKEL
                     QSQGYKLPEYPENPKDDKEKDIKSRYDKVKGSAVNPVLREGNSDRRAPLSVKAHTRKH
                     PHKMGAWSSDSKSHVSHMKSGDFFSNEKSLTTTAATEVRIEFVGQDGKATVLKPKVAL
                     QAGEVVDATFMSVKALRTFLEEQIQDSAKQGVLFSLHMKATMMKVSDPKIFGHAVTVY
                     YKDVFAKHGETLKKLGVDPDNGIGDLYAKIKALPEDQRKAIEADIQAVYKQRPPMAMV
                     NSDKGITNLHVPSDIIIDASMPPVIRDSGKMWGPDGKLADTKCVIPDASYAPVYREVI
                     DFCKKHGALDPKTMGSVPNVGLMAQAAEEYGSHDKTFKAPGNGTIRVVDASGKALLEH
                     KVEEGDIWRMCQVKDAPIRDWVKLAVARARATGAPAIFWLNKDRAHDAQLITKVNQYL
                     KEHDTNGLDIRIMTPADATRLSLERIKEGKDTISVTGNVLRDYLTDLFPILEIGTSAK
                     MLSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVAN
                     NPAAKMLADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAQQTQDKNL
                     AARFVKVAKEMVDNEAKISGELIAAQGKPVDTGGYYHPDDAKSSKAMRPSTTLNAIID
                     AIA"
     gene            complement(14992..17238)
                     /locus_tag="PK614_00365"
     CDS             complement(14992..17238)
                     /locus_tag="PK614_00365"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRB80700.1"
                     /translation="MSMDLAKKLYAKMPEVFAKARKQFGRGLTLADKILVSHADNFDA
                     QTWERGKAMLALRPDRVAMQDATAQMAMLQFMQANKEKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENREVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLNGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDEKMVAYMTITD
                     RADLATFAQSHRDLLVADPEVYQSPEKYYDQIVEVDLSTLEPHVVGPHTPDLARPISK
                     LAAEAKAKGYPVELKAALIGSCTNSSYEDISRSAHVAEQGLKAGLKAKASFLISPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGISETLSFLASPEVVTAYAITGDLGFDPVNQMLKGADGKEFKFQPPQGAELPVKGF
                     AKGEEGFVAPAESGEGLTVDIPPTSERLQLLQPFPRWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGQWLKFRGHLDKISDNMFLGANSAFTSEPGRGTNVFTGEANLTIAQIARDYKS
                     KGIGSVVVGDENYGEGSSREHAAMSPRFLNVKVVITKSFARIHETNLKKQGILALTFA
                     DPKDYDKIEQQDRISVTGLNNLAPGKPVQITIHKADGKALTIQTNHSITEQQIAWFKA
                     GSALNALN"
     gene            complement(17353..19173)
                     /locus_tag="PK614_00370"
     CDS             complement(17353..19173)
                     /locus_tag="PK614_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRB80701.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKSNEIPYGSGLIAIPVYKSIAEATKHHPTLNTSLVYIGADRAMKGGMEALDDS
                     HIKVVSMITEGVPEKDAKILGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIAVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPPTFGALGPAIKETYQDLLKSGQIKEPVEPVTLP
                     RLPKTIEEAMKADEVMVSPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGMIMIGPRF
                     GGAVTDAGRFFKYAVDNKMSVDEFLAYMKKNHGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLNIKTPCLDFALEVEKITSVKKDNLILNVDGTMAAVLVDIGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            complement(19216..20415)
                     /locus_tag="PK614_00375"
     CDS             complement(19216..20415)
                     /locus_tag="PK614_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HRB80702.1"
                     /translation="MAKVLEGPGMGLMKKWGINVPNYVVVTSVDELTRLGQANEWLKK
                     SKLVAKAHEALGSRFKLGLVKIDLDFKAAEAATKEMIGRQVGSITVTQVIVSEMIPHK
                     EEYYCAVKSTREGSEILVANCGGIEVESNWERVKRLCLEIGQAPSPESLEKLSKEAGF
                     SGPLAKKMAAFAGKMFACFDSEDAQYLEVNPVVLRAADEELVALDAVTLLDGDAKFRH
                     PDWNFAFAAEFGRAYSKQELEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIRNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSEGKLKNVKIWVRRGGPREKEGLDAMRALKEEGFDINVFDRNTPLTDIV
                     DKALQAK"
     gene            complement(20607..21470)
                     /gene="mutM"
                     /locus_tag="PK614_00380"
     CDS             complement(20607..21470)
                     /gene="mutM"
                     /locus_tag="PK614_00380"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRB80703.1"
                     /translation="MPELPEAEVAARQLRERVVGATVRDCWIGREDIVREGLPSLEQY
                     RQAKISGVERKGKSVILHFLCGKEPRFLVAELGMTGLLLFRSTSTKHPHHTHFILHLD
                     GCSEPDIRYWNPRRFGRLSLLDQAGLDRYVARRFGYDPLTISHEQFLCVLRATRSRLK
                     SLLMHQQVIAGIGNIYANEILFRSCLHPDQPANTIPGKSIVRLHQVMGEVLREAIAMG
                     GSSVRDYFAPDGTEGKYKSRHLVYAKAGEPCPNACGTVICRSVGERSSFYCPTCQRDG
                     RRRATRPEKSR"
     gene            complement(21660..22082)
                     /locus_tag="PK614_00385"
     CDS             complement(21660..22082)
                     /locus_tag="PK614_00385"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HRB80704.1"
                     /translation="MINKRLLVGNADDARNPPPQVSAVLMVAEEQNVTVPSRVIYAKI
                     PLKEFGEPAASALYEAVEWIAAHVADNRLLVCCRVGMGRSVSVVIAYLCCIEGMAYDD
                     AVKLVLTRRPGGMPLPRLQEKIQDVCRRRQARVNSSTP"
     gene            22210..22569
                     /locus_tag="PK614_00390"
     CDS             22210..22569
                     /locus_tag="PK614_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80705.1"
                     /translation="MAHTSLEAVEKRLASVQCAICKTNTFGVDRRTLQPDGECRAVCL
                     KCRYNFPVYTDMEFYLRTQPDVPYRLKEISCPSCQHRGVSLDFRITMSVREAIYFVTC
                     SACKTQFPERSSLEAFE"
     gene            complement(22806..23891)
                     /locus_tag="PK614_00395"
     CDS             complement(22806..23891)
                     /locus_tag="PK614_00395"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRB80706.1"
                     /translation="MFTRNGQVGSRVYFEDPQLRAGYLVYYLPVNLAKVQMLLDELQP
                     VLPVAQDHDFRVLDLGGGPGTGVLGVLDWCLSKSARPPSTLRMIAVDRSPQVLRVSTE
                     IWQAYSEQQRDQSIPPLNTVECNLERSLPSAIRDGGGVNGYQLIIVQNLLSELFVGSM
                     DPVGQRTALVGELLNYLAPEGSLMLIEPATRPASRELHQVRDNLLAGQYCSVFAPCLH
                     DAPCPALLKPDDWCHEERKWETPAWIAQVDREVGLLKDALKFSYVIFRKDGKTLVPRD
                     PDYHRVVSELRVMKGEKRVWLCDHAGRSEIGRQDKERSSSNAAFDDWHRGAIIRVSDI
                     VRKERKGRPAMVGRIISSGVVEIVRPA"
     gene            24078..24326
                     /locus_tag="PK614_00400"
     CDS             24078..24326
                     /locus_tag="PK614_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80707.1"
                     /translation="MTDPILQAYLEVEMAMERFTLVLHDHVDHLRKTEAPGSDKLHRM
                     ANGTKAMRDSASIYLSYAKYVAHGMPESPDLVEEDLQG"
     gene            complement(24420..25766)
                     /locus_tag="PK614_00405"
     CDS             complement(24420..25766)
                     /locus_tag="PK614_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB80708.1"
                     /translation="MSEALEPKQKTNPQSDPTTSVSAPKAETKKDPHADAKRQKVVTP
                     EYMFLEAPRTKEFITGSEAAKEAIRRSNVDLAIAYPITPQSETMQLVGVLYGEGYVKE
                     YYRGEEEVGVMAAIAGGSRAGVRCYTATAGPGTLRGLEGIASWPGHRLPVVAMFTCRV
                     VNAPLAIQPDNIEVSYLLNCGMIVFHAENQQDMYDFTLAGFIISEKNDVTLPVGVCCD
                     GFFVTHARGYVRMQDRGMKLPPREPWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNI
                     HAVWQQEVWAAVERSRKYINQYMGGLLTAENVDDAEAVIIASGSAAAQSREAVRICAE
                     KGIKIGLIKIRSLRPFPTQELRKLCGKAKLIVVPEFNYVGWLAKEVATAIYGFSNAKI
                     IGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAISQVMGASAADHEAMGHFMRSI"
     gene            complement(25805..26563)
                     /locus_tag="PK614_00410"
     CDS             complement(25805..26563)
                     /locus_tag="PK614_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HRB80709.1"
                     /translation="MTQDTRERIIVPGPAGFHPPSAAQLGVALPDPGEGLYYGLLEPN
                     EDIVIEEMARKMLTSPNATIFPGPLVLWAWNNHAVEKAKAVLEIAAQIPEVMIIPMPD
                     YRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCTMA
                     ICAEQGHEDAMLTIRDSDTLKLKRTAQIFKKVREEMGIKLPDNGENVRFTGTQSKVHN
                     GKTHTNPMTFMPSAAGVGSAATFGHSAEQMKREG"
     gene            complement(26591..26914)
                     /locus_tag="PK614_00415"
     CDS             complement(26591..26914)
                     /locus_tag="PK614_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRB80710.1"
                     /translation="MYLVAHIDVEICASKSCKLCTQYCPEANTIQYSEEMGKEKGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPMAALTDNITYSDKSTTAVLA
                     SPVVG"
     gene            complement(26941..27654)
                     /locus_tag="PK614_00420"
     CDS             complement(26941..27654)
                     /locus_tag="PK614_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRB80711.1"
                     /translation="MAKRFNIRMAGVGGQGVVTGSHILSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKEDGIAL
                     INNDGPMKLHRDQARELEERRARLYYFPATKLSLEVAGMDLCTNMALMGCIGAITGLT
                     TVEALEQSVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIDKNVAVIQAGWNYAVD
                     HGWAAPTVKRSEIRATVSA"
     gene            complement(27678..28577)
                     /locus_tag="PK614_00425"
     CDS             complement(27678..28577)
                     /locus_tag="PK614_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB80712.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMLQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKNVENDRYQFSEYITDEAKQFL
                     TERYGYKEFLPKPAAPAPGLPKA"
     gene            29043..29291
                     /locus_tag="PK614_00430"
     CDS             29043..29291
                     /locus_tag="PK614_00430"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRB80713.1"
                     /translation="MPIRVLVPGEEHGEQHAGGVHKRPPIPDGSLKAECPKFMSHGPC
                     GGVRKGGFCEVYPEMTCPWVTLYNKLNELGQLEWMKQV"
     gene            29316..29744
                     /gene="queF"
                     /locus_tag="PK614_00435"
     CDS             29316..29744
                     /gene="queF"
                     /locus_tag="PK614_00435"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRB80714.1"
                     /translation="MTAKGSKKRKPAGATTKLGYNERHAKSGISAPLPDIETFPNQYK
                     GYEITIVIPEYTAICPKTNLPDFGTITLHYKPDKHCLELKALKTYIHAYRNVGIFYEN
                     AVNRILQDIVRACRPINATVTGEFAARGGLRSVIEAKYPA"
     gene            30009..30425
                     /locus_tag="PK614_00440"
     CDS             30009..30425
                     /locus_tag="PK614_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80715.1"
                     /translation="MRTRSMGATIVQAMVGLFVVISGTYDMSVQTAEAIPAFARKYTL
                     NCTTCHTAPLQINTFGERFLENGYQLPGTEDGGVTGKKNLSAPRLANLYAQIEAACRV
                     GTPDNLPDLCGHLIPEHRAVCALMREELNRAGRSAA"
     gene            30454..34749
                     /locus_tag="PK614_00445"
     CDS             30454..34749
                     /locus_tag="PK614_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB80716.1"
                     /translation="MRRLLQRLRQSVRRATRQPATDPGDPQTERQEIRPSNKAESLDE
                     GRALDAVLCSLDEGLCIVDAQWHILRLNPQAEILFAAAAHDLKGRPAYQLIAPGPEEF
                     RHECLVTDSSSPPLKSGLPYRTDNGLLLRQDGQLMPISLTVTPMSHDGVVSGTVLTFR
                     DLTAQKEADSHQVEHSTLLRRLQAGMVGLASNQAIYRGQLTEAFHLITRVTTQSLHVA
                     RVSIWLYASDHAVLRCADLYEQPGNQHSNGIKLPAALYPQYFSSLEAGEMVVADEAQS
                     DPRTVEFAAQHLLPLGITSMLNVPIRAEGSLVGALHCEHIGAPRVWTTEEQQFAASVA
                     NTVSLAVEAADRHKAETETKHAEQFLDSVIENLPMMVYVKEAEQLRFTRWNRAAEALT
                     GFSRELILGRTDHDFFRKEEADRFTRHDREALRSGRLIEVASEEMHTQHRGVRLLRTK
                     KLPVMDEQGRPRFLLGIAEDITDRTTHESSLHESEEKYRELFESSRDAIMILSAPNWN
                     FTACNPATVQLFGARDAEHFTSLGPWDVSPEHQPDGEESMVKAPKMIGIALQEGSHNF
                     EWLHKRVGGPAFLASVQLTRITLKGPPGLQATVRDITEQRRSEAALQAYSTFQKAMLD
                     NAGHAIISCKPDGMIQVFNPAAEALLGYNADELVGKQTPGIFHDLDEVVARARQFSKE
                     LGTTIEPGFDVFVEKCRRNLPNKHEWAYVCKDGTRKTVLLNVTALREADGRITGYLGI
                     ASDITPLKLAAQELVSAKEAAEAANVAKSRFLANMSHEIRTPLNGVLGMTELLLNTSL
                     SAKQQSLLETVRRSGVALLEVINDILDFSKIEAGKLELEQVEFGLRQVVEEAVEIFAG
                     SAGNKKLELTCFIPADVPDSIVGDPVRLRQILLNLIGNAIKFTTQGEVAVAVALTKQH
                     ADTFTLACQVRDTGMGIPHTAQAQLFTAFTQADGSTTRRFGGTGLGLAIVKQLVQLMG
                     GEVGLVSAPGQGTTFSFTFTVTRGSAHAAYPPLAEHHLAGRQILIVDDTRTNLEILAT
                     HLRNWGATVFTAESADDTLLQLEHFRHRQHRIDMAILDLRMPDRDGVDLARSIKQQTA
                     YRSLPLIALSSVEGLSDESDATHQLFHSVLRKPVRQSLLKDCLTQVLGGSAGTVTPSP
                     QSSAAPRPRVEAHILLAEDNPVNQDVASSMLEMLGCRITIAPNGRAAVEAATTGRFDL
                     ILMDCQMPEMDGFTATATIRRQEATATDRRHVPIIALTANAMEGDRARCLAAGMDDYL
                     TKPFTVAQLHAFLTQWLTPQPVRASATDQASSTYTTDADEPTATEEAPIPTTIDKTAW
                     DAILSLQRPGRPDILARVLATYLHDSRQLVEQIRSAVQSQDSVALSQAAHRLKSSSAQ
                     LGVLATAAHCKELEALGRLARIDEAAHLLSQLIEAHQFACAAITSELQQRSAR"
     gene            34816..35634
                     /locus_tag="PK614_00450"
     CDS             34816..35634
                     /locus_tag="PK614_00450"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRB80717.1"
                     /translation="MPTYAIGDVQGCVASLRHLTQTIGFNPAQDRLWFVGDLVNRGPD
                     SLAVLRYIRGLGPAAVTVLGNHDLFLLAVATGLATLRRDDTLTQVLDAPDCGELTTWL
                     RQQPLLYREGSYLLVHAGLLPHWTIEEAQQFAMEAETALRGEQFSATLRALHPSGPLQ
                     WAPDLKGPVRLATIIKVLTRLRTCSDNGIMESTFSGPPKLTPQGFHPWFDVPNRRSAT
                     ATIVFGHWAAMGLHLTPNLLGLDSGCVYGRQLTAVRLEDRKVFQVRCEETRGGK"
     gene            35621..36160
                     /locus_tag="PK614_00455"
     CDS             35621..36160
                     /locus_tag="PK614_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HRB80718.1"
                     /translation="MEASEPPVTVDWPRYSSRALPTYRFLPGLTPHPHRDPLGHSFAE
                     REPRPRPFEPHQWSNSEDYLYAIDLYNFFYWWEAHEVFEGLWHACGRKTTTGNFFQAL
                     IQFAAAHLKRALGNEIAAHNLARSGLLRLQNSPTPYMGLDTVTFADDVTVWMGDNSRP
                     GVRIRLRHYGNGPSVNTIP"
     gene            complement(36316..37104)
                     /gene="panB"
                     /locus_tag="PK614_00460"
     CDS             complement(36316..37104)
                     /gene="panB"
                     /locus_tag="PK614_00460"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRB80719.1"
                     /translation="MTVPDLQKCKRDRRKITVVTAYDALFARIVEEAGIDVILVGDSL
                     GVVVQGKSNTLLVTMEEMLYHTKLVAGAAQRSLVIGDMPFLSYQVSQEEALRNAGRFI
                     QAGAHAVKLEGGAAVADRVSAIVKIGIPVMGHLGMTPQSVHQYGGYKVQGKGKDRAQQ
                     LLEDAQALEAAGAVGIVLEAIPAGLAKAVTEALTIPTIGIGAGPHCDGQVLVLYDLLG
                     LFDEFVPKFVKPFAHLKVDAVQALRRYKDEVEQGSFPSDSESYH"
     gene            complement(37104..37994)
                     /locus_tag="PK614_00465"
     CDS             complement(37104..37994)
                     /locus_tag="PK614_00465"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRB80720.1"
                     /translation="MTREPRRLKDVLAQGQFAVTVEYNPPKGTNLTHVVESAKALVGR
                     VHGVNVTDNTAAIVRAGSLPVCRVLYELGHDPVMQLTCRDRNRIAMQSDLMGAHILGI
                     RNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQLVTGLNNGKDYAGNKLDGSTAFTIGG
                     AVTPEADPLGPMLVKFEVKVRAGAEFFQTQAIYQPEQFKKFMEAVRPFKVKVLAGILL
                     LRNAKMAEFMNANIPGVCVPQEMIDEMRAVGDKRALDAGVEIAVRTIKAVRPHCDGVH
                     IMAIKSTERLPEILTKAELG"
     gene            complement(38178..39038)
                     /gene="folD"
                     /locus_tag="PK614_00470"
     CDS             complement(38178..39038)
                     /gene="folD"
                     /locus_tag="PK614_00470"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRB80721.1"
                     /translation="MAARIIDGKALAQQVRERLAVESAAVLAKTGVKPGLATILVGDD
                     PASHVYVRNKQKACELAGIYVDDHKLPASTTQAELLALIEKKNADPKIHGILVQLPLP
                     KHIESRVVLEAVSPNKDADGFHPYNFGRLVEGNPVFEACTPKGVIKMIESAGVSIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTKDLAAVCREADLLLVAIGKAKFVTA
                     EMVREGAVVIDVGTNKLPDGKLCGDVDFEPVSQKAGWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            39295..40326
                     /locus_tag="PK614_00475"
     CDS             39295..40326
                     /locus_tag="PK614_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80722.1"
                     /translation="MNSRTIRHNRFTRLLRRRRLGLALALAIGILLHHPWAWALQVSG
                     LESPHSFLADPATRSYYISNINGEADARDNNGFITKLSDDGRITAFKFIEGGRGDVTL
                     HAPKGMVVVDDVLYVTDLDTLRAFDKTTSKPLATLTLPRPAAGGAAQSLTDVASDGQG
                     HLYLADQGGNAIYRVDLAPTLTLSLYVSGAHLAGPSGIAVHPKTGHVVVVSYDSGKIF
                     DITPEGALTELASNGFFTARFQNLSGVDFDRWGSMYVSDGTKGKIWRMLPNGKFQVIA
                     EYLPGPSDVGIDRVNHLILVPYQDSNAAEVNGLESPTAASGDRAKRTLADYGFVEPKK
                     SDKEGSPRK"
     gene            40323..41021
                     /locus_tag="PK614_00480"
     CDS             40323..41021
                     /locus_tag="PK614_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80723.1"
                     /translation="MSQCVYCKQRKGKRSCPALTGLICSQCCGEHRLTRISCPPDCDY
                     LDTGSDYQQKRLGEQFAPVRRELYRQLSVAGGEKAAALFNLIEVVTFGYFHDRRDGQD
                     AEVFAAIQALRRTLSPLHVPSAPMPVFAERLKKEYDTFVKQQPQQVSDAGSAPEILDR
                     AVAILTEFSGQDFQSRRFLNGMIGYVKTFHPEIAEHLTKQHEAGRIVLPGQLTPPPQE
                     PTHVHGPECHHHHH"
     gene            41229..41804
                     /locus_tag="PK614_00485"
     CDS             41229..41804
                     /locus_tag="PK614_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80724.1"
                     /translation="MDLLVSLPKEDALAEPAWSSRHGGILSILWLHVLSVPMFGIYMG
                     ASPTLYLGGGGLLAFIAVAARLSVVRRRLQSAIATCGLMTALALLGHLSGGQIELHFH
                     FFVIMSVIVLYRDWLPFLVGLQFIILDHGVVGTLRPDLVIGHAQGQTHPSAWASIHGG
                     FILPNVRPCSTSGASMNSHKMRSGKARPELA"
     gene            41801..43090
                     /locus_tag="PK614_00490"
     CDS             41801..43090
                     /locus_tag="PK614_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB80725.1"
                     /translation="MIIETALDAVITTDATRTITGWNTQAESMFDVPRTEAIGKSLTT
                     YVSASYPSTEPGPDTAYAGLVPGAILNRRSEMLGHTCQGTSFPVEIAMSCLAIGGTQQ
                     FTIFVQDISERKKQEERLRRAKEAAEVASLAKSQFLATMSHEIRTPMNGVLGMTELLL
                     TTPLNDKQRRYADTVHHSGTNLLHIINDILDFSKIEAGRLVLEYIPFGLRQMLTETIG
                     LYHEQARKKGLALTVTIAPDTPGTVHGDPHRLRQILTNLVGNALKFAETGNVAVSVRP
                     DEGDQGRVRIEVTDTGIGIPLHAQEKIFDSFSQADGSTTRKYGGTGLGLAIVKQLVRM
                     TGWQLGLCSTPGQGTTFWFTISVDRSTSPETESQQHSSDETTLTMATRQQPATRRRSL
                     TCMSRLAPSKPAVPSPLRRSPHWIRRKPSSCSSELRA"
     gene            42979..44127
                     /locus_tag="PK614_00495"
     CDS             42979..44127
                     /locus_tag="PK614_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRB80726.1"
                     /translation="MHVETCSIKTGSAFSPASLSTLDSKETLVLLFGAPGLIDAPHRI
                     REVLESFPRSRVMGCSTAGEIHGCEISDDSLVVAAVRFEQTPIRTAEAAVQSPQDSYN
                     AGCAIATQLKQPSLRGVLVLSDGLNVNGSELVKGLNDTLGEGVVVTGGLAGDGIHFKR
                     TWVLKDRTPHSGYVTAIGFYGDHVRLGHGSKGGWDKFGPERLVTKSTGNILYELDGRP
                     ALQLYKEYLGDRATGLPATGLLFPLAIRTSQSDGKMLVRTILAVDEATQSMTFAGDIP
                     EGVFAQLMRANFDRLIQGASEAATLTANNHIGSHATSPTLSIAISCVGRRLVLGERTE
                     EEVEATLEILPKDSKQIGFYSYGEISPYASGACDLHNQTMTLTTITEH"
     gene            44133..47765
                     /locus_tag="PK614_00500"
     CDS             44133..47765
                     /locus_tag="PK614_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB80727.1"
                     /translation="MHPLLKRQLKRLGLDDATAPQSLASWQQLLERVSQSYQESDQGR
                     ELLERSIALSSQEMQELNEQLRRTSESKLAGERDKLQTVLRSMGDGLCVVDGQWNIVL
                     LNPEGERLWGLTEKEVVGRRLHDMISLSYGTTLTEPIFTQILLDETAQGDSFRTDDGL
                     LTATTGRSFPVACVLAPIVHDHHFAGAVLVFRDITDRKQAEDVRRHTESLLRRQQSAL
                     LELTRNSVLQSGVLEPALQEITRATATTLGVGRTSIWLLSEDHGSIRCKDLYESSRNS
                     HSLGIELQSADFPEYFRELLSERVIDASDARSDPRTREFTSNYLEPLGIGAMLDIPIR
                     FNGKLVGVLCNEHIGPPRPWMLEEQQFGHAIASLVSLALEAVERLHAEGALRKSEGRT
                     RLIIDTALSAVISMDEKGSIIGWNTQAEQIFGWTRQEAIGRLMAETIIPPEQREAHQR
                     GLRQFFKTGEGPILNKRIEITALRRDGTEFPVELAVTPLRLENAYTFTAFVVDISERK
                     KAEEALRTSEARLTMTVQGSHIGIWDWNLTTGAIYFSPQWKNQLGYDDTTLPNNFDEW
                     RTRIHPDDQPFVQETIQTCLDGQRSQFELEHRLRHRDGSYRWILSRGSLIRDVYGVTA
                     RMVGIHIDTTDRKQVEQELRAAKEAAEAASKAKSEFLANMSHEIRTPMNGVLGTTELL
                     LNSALTDKQRHLASTVHRSGRTLLAIINDILDFSKIEAGKLDLECVDFDLQPVLEESL
                     ELFGEAARRKQLRLSQHIDDAVPRYLKGDPVRFRQILMNLLSNAMKFTETGGVSLSTE
                     YLEGTTSQALLRFAVTDTGIGIPPAAKSHIFDAFSQADGSTTRRYGGTGLGLSIAKQL
                     VGLMGGTITVESTPGSGSTFAFTTRFDLQPLGSGLSTKLAPYMQWAEPLVSSGQTSDT
                     PCPHASEAGAAAPSPKQSGRILLAEDSPVNREVAVGMLEQLGYEVEVAENGRQALAAA
                     ERDRFDIVLMDCQMPEMDGLTATGEIRRRETASGRRRLPIIALTAHAMQGDREQCLTA
                     GMDDYLSKPFTQMQLQGIVRKWLSHHEPPAQAAQLNAAAPGTQSVPLQTTTEPAADTS
                     STAPGMDLKALEGIRALQRPNRPNVLASVLRKYLDNSRESVDALRDAIRASDPMALEA
                     MAHRLKSSSAQLGAIAVAARCKELEVMGNRKHLVDADLIFVQLESDYRAACTVFRNEI
                     AKENQT"
     gene            47762..49009
                     /locus_tag="PK614_00505"
     CDS             47762..49009
                     /locus_tag="PK614_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB80728.1"
                     /translation="MNPLIPGRAPYALITDDDIIIRMFAREALEQAGWVVEEAQNGIE
                     ACEIFQKCPPDVVLLDVMMPGMDGFATCAALRRLPGGEHTPILIMTGLDDFESITKAY
                     DAGATDFIVKPLNAMLLTHRIRYMVRADQVLQELRESQATLTQARDAAIEGARLKSEF
                     LATMSHEIRTPMNGVLGMTDWLLDTPLTAEQLDCAQTIRSSGDALMVIINDILDFSKI
                     ESGKLSLELLDFDLPTFLDRVLALFGERAQRKGLLLTSRIAEDVPSGLCGDPARLQQI
                     LSNLLANAIKFSEHGSVSVLVELDQRPAEQGLEFPATRYGELSAQQERVTHIRFSVSD
                     NGIGIEPSAFSKLFQPFVQADGSTTRKYGGTGLGLAISKQLVELMGGHIGAESEPGSG
                     SVFRFTVPLQQQLPNSLSTRQAA"
     gene            49343..50236
                     /locus_tag="PK614_00510"
     CDS             49343..50236
                     /locus_tag="PK614_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRB80729.1"
                     /translation="MHCLTFDIEEHFQVSRFDSPIRRRHWGSFESRVTPNTCKVLDLL
                     ARYQTRATFFVLGWVAERHPGLIKQIAECGHEIASHGYGHELVTAQTPELFRADVRKA
                     KQILEDLTGSPVQGYRAPGFTITRETLWALPILAEEGYTYDSSIVPIRHDHCGLPGSD
                     PWHHLRQTSSGPIWEIPPSTVNLGGVRLPIAGGSYFRLLPFPALCGLLRRTEQKGRPL
                     VMYFHPWELDPLQPHMEGPLLSQFLHYLNLDKMEFRISTLMNTFRFGPIAEQIDLSPI
                     MPLDPTIFPALPPAEIAQDVA"
     gene            50395..50970
                     /locus_tag="PK614_00515"
     CDS             50395..50970
                     /locus_tag="PK614_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80730.1"
                     /translation="MHHGSRNRRQQSPNTTGLAWALACIILLPAAAAGQSLDDTVQFI
                     SDMANTHGFVRSLSCRNPKAGKPTKITEIYSVIPTETKLGTIELNHGHDEVNTGFTHF
                     DLHDIDTIEYQGQDTKENNLVLYGVRITCKSSGPCIMKTSFCTGAVTELEAQFPEDTL
                     LFRSASHAERMTKALAHFLGLVRAQQQNQPF"
     gene            complement(51036..>51410)
                     /locus_tag="PK614_00520"
     CDS             complement(51036..>51410)
                     /locus_tag="PK614_00520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB80731.1"
                     /translation="GFMIQGGDPNTKDSLKKGAYGQGGPGHNVKAEFSDLPHKRGVVS
                     MARAQDPDSAGSQFFVVVEESRFLDRKYTIFGEVVKGIGVADKIVALPRVMCQTGAPS
                     PADKGPCDNPIDRVEMTVTIIE"
BASE COUNT        11495 a        15845 c        13276 g        10794 t
ORIGIN      
        1 gatcactttt tcacgcagga caccctgctg atcaatgatg taggtttcgg ggacacccat
       61 caatttgtag cgcttgtcgg tctggcccca ggaatcaacc agtacgggaa acgtgaggtt
      121 caagcccttc acgaacggag gtatttcttt cttcgtcgtc acccggtcga tgctgaccgc
      181 gagaatcacc aacccgtcct tttcaaaatt cttgtacaaa acctccatcg aaggcatttc
      241 ctcacggcac ggcttgcacc aggtcgccca gaaattcaaa aagaccactt tgccgcgata
      301 gtccgataac cgcagctgct tctcgtttaa gtcgggcaat tgaaaatccg gtgcctcttt
      361 tcccaccacc aatggttcgt acttggaact ctgtagccag actacgagaa acatcacacc
      421 cagaatggcc gcagcggcta ttaccaccag ggtacgggac gggccggttc gcggcgcagt
      481 attggcaggg ctctggttga ttggatctgt catggtctag gcgtaagcat gcagaccgga
      541 gagcagaaaa ttcactcccc agaaacagaa aatgaccatg agaaagccaa agatcgcgta
      601 gatcgccgct ttatggcctt cccacccccg cgtcatgcga gcatggatat aggcgccata
      661 gatcagccat acgatcaacg accaggtctc cttaggatcc caactccagt acccgcccca
      721 ggcatagttc gcccacattg ctccgaggat gatcccgaag gccagcagcg gaaaccccag
      781 catgatcgcc ttatagccaa gctcatcgat ggtttcgaga tcgggaaagg cggcataaaa
      841 ccgcgactga ctgccacgat tgaccgcccg ttctttgaag agatacatca ggcccaatcc
      901 accggccatg gcaaacgccg cgtagctggt caacgtgacg gagacatgga tgtagatcca
      961 gtagctgttc agggccggaa cgagcggctc tgccgtctgg tatcgatagg gcaagagcga
     1021 cgcggcgccc atggcaataa acccgattcc caccacaaag gcaccgacgg ccttaatcct
     1081 gtagcggaat tcgagaagca cgtagcccaa gataatggcc caggacacat aggccattgc
     1141 ctcgaactgg tttgaccagg gcgcgaaggt gcctgaatgt tccattcgct caaatgcccg
     1201 cgtcgtgagg gccaacgtat tcagcaccca cccgaacacg gtgacgaggg ttgccacctg
     1261 ccccaattgt gtggcccatg cgccatcacg ttcttcaagg tcctcaatcg gatgcccggc
     1321 tggaaccatc tgcatggcag gacgttttgc gaacagatag gccacataca accctaacgc
     1381 ggctaaatac agccagaacg tcatatcgaa gaggaacagt gatcgcatca tacggacctc
     1441 cgtctgtcta tcctagcgtg cagcgcagac cgaataaaac gttgctttcg gtcgcgcggc
     1501 agcgatcttc aggattgagc ctgagcgtta attttctccg tcaacttcct gaactctttt
     1561 tggaaatcga tctggctttt gtgcgttgtt ccgccaagat ggagcgtgac accggactcg
     1621 ccgttaatga tcttcgccca taaccgccga tgataaatca gcgacgacag agtcatgccc
     1681 accaccagca tcgtacaccc gatccagacc atattgatgc caggatttct ggcaatctgg
     1741 agcccggtga acttcttcgg ctgatatccg atgaattcga attgatatgc ggagtccttg
     1801 atctcgaaga ggtcgggata atgatagaac acccagggag tggactgcac gctactccgc
     1861 tcatcaaccg ccagccgaat cgcgggattg gcatgctccg cggtttttga aaacaccttc
     1921 ttctcggtcg aattaaacgc gaagtcggcc acaaaatccg tcatcttcat cttcaatgga
     1981 aggccgtcga cggctttttc cttattccac tccagatcga ccgtagcgat aatcttgtcg
     2041 ctgcccttct ccttaatgtt gattcgagcc gcctcgatct gatcccacgc atccccatag
     2101 ctggattggt aaaaccagat ccctttgtag accaagggat cgttcaccgt aatcgtcttg
     2161 gttgtagtgg gagtgccctg atcgatcacg gtcaaggtgc tgttgtagga ttttaccgag
     2221 ccgttctcat ggtaatcgat ccagaactta tcgacacgaa gatcgaagtt gccacgcggg
     2281 atatggtagg tctgcccttc gaggcatacc ccgaactcct gaaacccata atagctacct
     2341 agtagccccc cgagcacgat gaccgtggcg ctgaggtggg ccacatgcgc gccgacccgg
     2401 cccataatcc ccttggtggc gtagaccgtc acctcgccgg gatcgctctt ggccagcacc
     2461 cgataacctt tttcagcgaa gagctttaca agtcgctcag ccacagcgtc tctcgattga
     2521 ttgagtgaga gggtggtctg ctgcttcatc ccctggataa aggccagcga cacactgacc
     2581 ttatcctgac gcatggaccg ccatacactg ggaaaccgtt tgtggaaaca agtgagagaa
     2641 ttgacgcaca ggagccccag cagactggta aaccaccagg tgtgatagac atcggtgaaa
     2701 cccaatcgca ggaaccagcg ataggcttct tctccatatt cctgcacata ggtttccggg
     2761 cgttcgccct gctgaatgac cgtgccgatc gtcgcagtca tggcgagcac aatgaacaga
     2821 aacatcgcca gcttgatcga agcaaaaaaa tccacgacct cgcgtgaaaa ctcctcccag
     2881 ccaaggccgg aggtgcgcgg agcggatgcg ctcacatcgg atgctgcatc gggtttatcg
     2941 ttcatgaatc aacataccta gctgcttcga cctggctgtc atacaaatcc catcatcata
     3001 cccaatgacg agctgcgtgt ggaatcctgc gccaatgcag gtcggtacca cgaggctgga
     3061 agtgccgggg cggagcgttt atgatgaaaa atgttccaaa tacgcgaaaa gtaagctatc
     3121 ttacaagtcg tcttaaagga ctgtcaagca agacgcttgt ccggcgcgag actcaaccaa
     3181 ttccaccaga acggtggaca aaaactgacc ggtcagctac tattgcctta gttttttgcg
     3241 gagttgcctg atcatcttaa gcgtagcggc ttccacaatc agtcgccgta ccacctgact
     3301 tcaatcgccg catcctcgta tcacttcaat tccaggggga acaatgagac ataactacct
     3361 gttcacgtct gagtctgtca ccgaagggca cccggataag attgccgatc agatctcgga
     3421 cggaattcta gacgccatca tcgctcaaga caaattttcg cgagtcgcct gcgaaaccat
     3481 tctcacgacc ggcatcgcat tcgtcgccgg agaaatctcg accaaggcct atgtggagat
     3541 tcccgatatc attcgcgacg tgatcaagga cgtcggctat acggacgcct cctggggttt
     3601 cgattccaac acctgctcgg tcttgacgtc gatccaccaa cagtccggcg atatcgccat
     3661 gggagttgat tccggcggcg ccggcgacca gggcctcatg tttggatatg ccaccaatga
     3721 aacgaccgag ctcatgccga tgccgatcgt gctggcccat cgattgacca aacgtttggc
     3781 cgaagtgcgc aagaaaaaaa ttctcaaatg ggtgcggccc gacggcaaat cgcaggtaac
     3841 cgtggaatat aaggacggca aaccttgccg cgtcgatacc atcgtcgttt ccacacagca
     3901 cagccccgac gtgaccaaca agcaaatcga gaaggatttg atggaacacg tgatcaggcc
     3961 gtccatgccg aagggtctgt acgatccgac cagcgtgaaa caccatatca acccgaccgg
     4021 ccgattcgtg gttggcggac cgatgggcga taccggcctg accggccgca agatcatcgt
     4081 cgatacctac ggcgggcacg gcagccacgg cggaggggcc ttctcgggga aagatccatc
     4141 aaaagtagat cgctccgcct cgtacatggc tcgctatatt gcaaagaaca tcgtggccgc
     4201 aggcctggct tcaaaatgcg aagtccagct ggcctacgct attggagtag ccgatcccgt
     4261 ctccgtattg gtcgacacca aggacaccga gaaggtagca cccgagagct tggacaagct
     4321 ggtccggaag catttcccat tgaccccgcg cgggatcatc gaccatctca agctgcgtcg
     4381 ccccatcttt aggaagacgg ccgcctacgg gcacttcggt cgcaacgaac cggagttcac
     4441 ctgggaaaaa accgacaaag ccagagcgtt gcgcaaggac gcgggcctct aaacgcctac
     4501 agcgcgtcgc accagccaag ggggcgggtc gcaagcccgc cccttttttc tgcattcacg
     4561 cattactcga tatcagtcac aacacaggag gacgaccagt ggactacgac gtgaaagaca
     4621 tgggattagc tgatcaggga aaattgaaaa tcgaatgggc agaagccacg atgccggtac
     4681 tccggctcat tcgcaaacgc tttgaacggg aacagccgtt taaaggcatc cgcgccaccg
     4741 cttgcctaca cgtgaccacc gaaacggcca acctgatgaa aacgctcaag gccggcgggg
     4801 cagatgttcg cctctgcgcc tccaacccgt tgagcactca agacgatgtc gccgccgccc
     4861 tggttcggca cgaagggatc ccgacctttg ccgtcaaggg agaagacaac aagacctact
     4921 accggcatat tgaatcggcc atcgcccaca agccgcacct gtccatggac gacggagccg
     4981 atgtggtctc ccatctacac tccaagcgga aagacttact ccgcaacgtg atcggtggaa
     5041 cagaggaaac caccaccggc gtcatccgcc ttcgcagcat ggccgagaag aaagttctga
     5101 aattcccggt catctccgtg aacgatgcgg atacgaaaca catgttcgac aaccggtatg
     5161 gcactggcca aagcaccatg gacggcattg tccgtgccac caatcggctg gtgtgcggct
     5221 ccacgctcgt cgtcgccggc tacggctggt gcggccgtgg cattgccacg cgcgcacgtg
     5281 gcatgggcgc caacgtcatc gtgactgaaa tcgatccatt gaagggcctc gaagccgtga
     5341 tggacggatt ccgggtcatg ccgatggatg aagccgctcc ccttggagac ttttttgtca
     5401 cagtcacagg caacttgaag gttattcgcg gcgagcactt tgcggccatg aaagacgggg
     5461 caatcgtctg caattccgga cacttcaatg tcgaactgga cattcctgca ctggaaaagc
     5521 tcagcaagaa aaaactcgcc gtgcgctcgg gtgtggacca atacaccttg aagaacggcc
     5581 gtcgcgtcag cctcttgggc gaaggtcgtc tcgtgaacct agccaccgct gaaggccacc
     5641 cctccagcgt catggacatg agcttcgcga atcaggcttt gggcgccgaa tatattgtga
     5701 agaactacaa gaaattggaa aagaaggtct atccggttcc cgccgacatc gataaggaaa
     5761 tttcgcggct caagctcgcc ggcatgggcg tgtcgatcga caagctcacc aaagagcagg
     5821 tgaagtactt ggcctcctgg gaaatgggaa cctagtttta tcggagaagg ccggagctgc
     5881 tctcgtggcg gctccggctc atccaccgag cagttctgag cgtctgagcg aactcctcat
     5941 gccactccgg acgctcctac tactctttct ctctgctctc tataactttc tcgcagtcat
     6001 accgaatacc tggcgtggcc tctgacaagt attggaggac gattgtcatg aagcgattac
     6061 accacagtag gcgcaggtgc agctccggcc aaatagatac agaaagcgtc aaacctagac
     6121 ttccccaggc ttcacttgct gaagcacgcg gatagctgac agaacatggc gaaccttgcg
     6181 cagactaacg tgaattgacc atgggaagtt cgcgagatcc ttcggcctcc tgctgaatcc
     6241 gctgacgctc ggctttcgac tcttccagca cactgtgcaa taactcatcg ctcagctggg
     6301 tcaattgcgc tgcagcgtct ttcatctccg catgctccac cgcccgcgac aatacttcgg
     6361 ccttcgtcgc ccccttcttc tggcccaaga aaggcttgat gatgagatag gccacatcac
     6421 ggaacggcat gaaacgcaag aatcggcgca gcagcttgaa ggtctgaatc gggtatcggg
     6481 tgagcttata aataaacagc ttcttcagcc cctcttggcg aactgaatta atgactgccc
     6541 ccggcaaaca ggtcgggtca atctccgaac acttaaaata cttgtaccaa tccttggcgt
     6601 cgctcaccaa tccacgcttg atatattcct gccacagagg cgtgccccga tagacgcaca
     6661 gccgattgaa tccaaacgtg tccaaaggca atttcgaggc gaaatcaaac gtcttctgca
     6721 tgtcctcgac ggtttcgtcg ggattgccca ccgtaaagaa accgtgaacg atttcgatcc
     6781 ctgccttctt ggcgtttcta acggcggtct caacctcggc gagcgtctgc tccttctgga
     6841 ggcgatccag aattttctgg ctgccgcttt cgatgccgaa catcaccgtc ctgcaatgcg
     6901 cctgagccat ggcggggaag aggtgctggg ccaccgaatc aacccggcct tcgattcccc
     6961 attggattgt cagcttcgca tcgatgatcc ctttacaaat cgcatcgatc cgcttcggct
     7021 gtagtaaaaa gtgatcatcg acgaaataga cagatccgta cccattcgcc tcgaggtact
     7081 tcagctcatc aacgacgtgc tgcgcgctac gcgcccgcca cttcccctca ttgaaaatcg
     7141 ggatgtcaca gaagacacac ggccacggac agccccgcga agtctgcatg gtcgtaaatc
     7201 gctccatcga cagcacggcc ggcacgtcca acggcatcga ctcgacaaag tctaattcca
     7261 ggctttcccg gtcaggaaat ggccactggt ccaagtggcg ctccatcgtc ctgttggggt
     7321 tattgatcac cttgccgtct ttcgcccaag tgacaccgcc cacctcttgc ggatcctcaa
     7381 gacgatcgag caaatcaagc aacaactgct ccccgtctcc tcgacacaca aagtcgattt
     7441 ctggacactg cagcttcacc aaacccgcgt tgagactggc aaatactccg ccgaaggcca
     7501 gcttgacggt actatcggcc gcccgaatct gacgagcaag aattttggca tagggatagc
     7561 tggtggtact gaggaagcta agaccaacca aggccggctt ccgccgtttg atctcttcga
     7621 taatgaactc attcggggtc tcaggatgcg cctgatcgaa catcacacat tcatggcccg
     7681 accgcttcaa taccgacgac aatgacataa tgccgatggg cggaaacccc atgacccgga
     7741 tacgcccgtt cttggctcga gtcttggccg gaagggcata aaactgggga tcacgcacat
     7801 gaataagaaa caccagcata gtaacgtcct ttcatcggga cagtaaccgg attcctcaga
     7861 ctgccaccag atcgcttcga gagcggagaa tccaggttcg aattgcagag aggccaaatc
     7921 actcaggcca ttcgaccccc ctgaggcgtc atacagtacc acgtctcgca ggagggaaaa
     7981 agggaagatt ctgacaaaga gaggggtaca ccggtgacat cggcgaccct caaccgttga
     8041 ccggcgatat ggtttgcgcg ctacaaacgc aaagagggcg tcggaccaga tccgacgccc
     8101 tccttagaag agacacctga ctagcgaccg agcgccgcct tcacggcatg gccgatctca
     8161 gcgggattct tcacgactcg tacaccagca gcttctagcg ccttcatttt ttccgtcgcc
     8221 gtccctttgc cccctgaaat gatggcaccc gcatgcccca tccgacgccc tggcggcgcc
     8281 gtgatcccgg caatgaaact aatcactggc ttcttgacgt gcttcttaat atactcggcc
     8341 gccttctctt ccgcatcgcc accgatttcg ccgatcatca caacagcctg tgtctccgga
     8401 tctttctcga acaagggcaa gacatccacg aatcccgtgc cgttgacggg atcaccgccg
     8461 attcccacac aggtcgtttc tccgagcccc aacgtcgaca gttgatgaac ggcctcatac
     8521 gtcagggtgc cgctgcgtga tactacgcca actacaccac gtttatggat gaagccgggc
     8581 atgatgccga tcttggcttc atcgactgtg atgacgcctg ggcaattggg gccgattaat
     8641 cggacatcgc gtccatgcag cgcacgtttc accttcacca tatcgttcac gggaatgcct
     8701 tcggtgatgc agataatcag cttgacacct gcatcagcag cttccaggat cgcatccgca
     8761 caaaatggcg gcggcacaaa gatcaaagag gtatcgcagt cggtcttcct cacagcatcg
     8821 gccacggtgt tgaagacggg gatcccctca acttcctgcc cggccttgcc aggggtcact
     8881 ccggcgacca tcttcgtgcc atacgctttg cattgcgtcg cgtgaaagga cccttccttc
     8941 cctgtgatcc cctgcactac cactcgcgta ttcttattga cgagaatgct cacggtcttc
     9001 tcctttacgc cgccttgccg gtcagcttca caattttctg cgccgcttcc cacagatcat
     9061 tggcaacctc caacttgagg ccggactccg ccaatagttt gcggccttca tctgcattcg
     9121 taccttggag acgcaccact aatggaacgg taatcttcac ttccttggca gcttcgatca
     9181 caccatgcgc aatgcgctca catcgtacga taccaccgaa gatattgata aagatgccct
     9241 tcacgttcgg gtccttgagc agaatccgga atccggctgc caccgtgtcc ttcgtggctc
     9301 cgccgcccac atccaggaag ttcgccggct cgctgcccgc tagcttgatg acgtccatag
     9361 tggccatcgc aagaccggcg ccgttcacca tacagccgat gttgccgtca agcttgacat
     9421 aattcagatt gttcgctgtc gcttcgattt ccagcggatc ctcttcattg aggtcccgca
     9481 ttttctgaac atcgtcatgt ttaaaaatgc cgttgtcgtc aaatgagacc ttgccgtcca
     9541 atgccaccaa ggttttctcc ttggtgataa tcaacggatt gatttcaacg agcgccgcgt
     9601 gtttctccat gaataggcga tagagattgc cgagcatctg cacgaaggga ttgatgacag
     9661 ccggttccat cgtctgcaag cccaatgcaa aagcgacgtt gcgcccattg tgcccctgaa
     9721 atccaacggc gggatcgacc gcttccttaa taatcttctc cggagtcttt tcagccacct
     9781 cttcgatttc catcccgcct tcggtgctgg cgataaacgt cggccaaccg gtgtcgcgat
     9841 caacaagaat gctcagatat aactctttcg caatattggc gccctcttcg agcaacagcc
     9901 gatgaacctt tctccccttc ggtccagtct ggtgcgtgat caaggtcttg ccgatcaact
     9961 ccttgacgat tcctgcgaca gcccccttct ccttcgtaat tttgacgccg ccggcctttc
    10021 cacgcccacc cgcatggatt tgagctttca ccacgaagac aggagtattc agttcacttg
    10081 cccaggcagt ggcggcctcg ggtgaggtaa tttccttgcc ccgtggcacc ggaaccccga
    10141 actgtgcgaa caattgcttg gcctgaaatt catgaacgtt cattgaatcc tcataagagc
    10201 tagtggctca tgacaaatga cgtagagcgt gactcccagc ggggtcggct tgcccggacc
    10261 agccgccatt agccattcgc cgtacgctct ggttcttttc gcgtataggc ggggagaatc
    10321 atcggagaga tcacaccgct ggagctgccg tgctgcttgg cctccgctcg gaatcgtcgc
    10381 agatgttgga gcgtgagctt cccttgtggc atggatccgg cgcgcgatgc cgctgtcaac
    10441 cctgagcgct ttccaaacac catgaggtcc agtagcgaat tccccatcaa tcgatttcgt
    10501 ccgtgcaatc ccccagaagc ttctccggca acgaagagat tcttcacatt ggtttccgag
    10561 tttgcatcga tcttcactcc gccgttctga tagtgcaacg tcggataaat gaggacggga
    10621 tccttactga tgtcaatacc gaatcgctca aactgcatca tcatggcagg aaaatgcttt
    10681 tccaccgttc ccggcccatg ctctgcatca agcaacggcg tatccaacca aacaccgaca
    10741 cgaccggaca tggtgcgaat accacggccc tcctcacact cacgaataat ggaggaggag
    10801 acgacatcac gggtatcgag ctcattcaca aatcgctcac ctttggcgtt caccagatgg
    10861 ccgccctccg acctgatacc ctctgtaacc aaggcgccga tcaattgctc gggatagacc
    10921 gcgcctgacg ggtgatactg gaacgtatcg atgtgatcga gcttcgcgcc catccggtag
    10981 gagaggcaca aaccgtcgcc cgtcgcgcca taatggttgc tggtggggaa tccttgaata
    11041 tgcagccggc cgataccacc ggtcgcgaga atcacagact tcgcagccac aaccacctgc
    11101 ctgtgattgt cgagatcctt aaagatcgcc cccgtgcaat ggctgtggtc atcactgagc
    11161 aactccactg cggcgcagaa ttcgagaagt tggatctttt gattcagcac ctcgtctttg
    11221 aggactcgca tgatctctag gccagtgtag tctgagcaag tcagaaggcg tggcttagag
    11281 ctcccccccc ccttcttgac gtgcagattg ccatcggcct gccgatcaaa aagcacgccc
    11341 agttcaatta accacttggc aatggaaggc ccatcctcga ccatgacctt gagtaattcg
    11401 tggtcgttct gcatatggcc acccttgagt gtgtcaagga aatgggtgac cggagaatct
    11461 tctggcgcaa cggaaatctg catcccgcct tgagccatca cactattgga gtccccaagt
    11521 cgcagcttcg tggcaagaat caccttcgcg ccggtaccgt gggcatgcaa cgctgccgca
    11581 cacccagcac cgccgccgcc gacgaccaaa acatccgttg tgtagtgtgg ggtcagatca
    11641 atattctcag cgattgagct atcaccttcg agaagggcag cgagttcgac gaccgtcttg
    11701 tcgttcgcgt tcgggccaaa tcgaatcgga cgataggcgc tggtacggta atcaggatga
    11761 tacttatgga tcagctggtc gcgatcggca ggggagaact tcgggatagt ctgtttacgg
    11821 cgagcgtccc gagtctggtg gacaatctgt tggagtgcgt gaatatccat ggtttcactt
    11881 tcagcacgga gcacagtctt cggacatatc tgcccgagac cggcggacgg cacgaggcat
    11941 tacttcactg tcgcgcagtg gtcggccagc tcctgttcgt tcattgcgag cactttgttc
    12001 cattcatcgt tgaaacggcc gtcttgaatt tccttgatac ggatatccag cccctgcggc
    12061 ttctctgtga aatgagcacc ttgcgcccgg ctcacgtaca gggcgaccag attgggggcg
    12121 atatcggcaa tacacaccgg tgtgcacatc ccacacatga cgcaatccat gaacatctcg
    12181 gagacgctct tgaaatcacc gaacactgcc ttccacaccc cttcccgcac atcgatcttc
    12241 tgagggcacg cctccgtaca ggcattgcaa ttgcggcaaa gcggcgcttc cggatagaga
    12301 ttgaagaggt cttgcttcgg atctttgagc gactggatgt cgtacagagc cttacgagcg
    12361 ggaaacggcg gcataatggt gaaagacatg ccatcttcta cagccatctg gcaggccagg
    12421 caggtccgga ccttgggatc atccttggtt cgatagtagg tcgcgcaggc tccgcagaat
    12481 ccgccgaggc agccggctcc acgcactact tcctggcccg tatgccacaa tgccttgatg
    12541 acggtgatac cttccggcac ttcatacttc ttaccggaga tttcaatcgt caccattttg
    12601 tggcgcaaca cttctggctg gtcgatcacg ttggtgtctt cttgtgccat gtttctctcg
    12661 tggaccgctg aaagccaggc gtgaggagat tcgcttcctc cctcacacct gacgtttaat
    12721 taacggatac gcccctacgc aattgcgtcg atgatagcat tcagcgtggt gcttggacgc
    12781 atcgccttgg acgacttcgc gtcatcaggg tggtagtacc cacctgtatc gaccggctta
    12841 ccctgagccg caatcagctc cccagatatt ttggcttcgt tatccaccat ttccttagca
    12901 accttgacga agcgcgctgc caaattctta tcctgcgtct gctgggccaa cgcctgcgcc
    12961 cagtagagag ccagatagaa atggctgccg cggttatcga tttctccgac tttgcgcgcc
    13021 ggcgatttat tgctctccag gaacttggca ttggcttgat caagggtatc cgccagcatt
    13081 ttcgccgcgg gattgttcgc cactttggcc agatgctcca gcgaggcagc cagcgccagg
    13141 aattctccga gcgaatccca ccgcagatag ccttcttcct ggaactgctg cacgtgcttc
    13201 ggcgcggatc ctcccgcacc ggtctcaaac agaccgccgc cgttgagcaa cggcacgatc
    13261 gaaagcatct tggcgctggt tccgatttcg agaatcggga acaggtccgt cagatagtcg
    13321 cgcaagacgt tccccgtcac ggaaatcgtg tccttgcctt ccttgatccg ctccagcgag
    13381 agacgagtcg catcggccgg cgtcatgatc cggatatcga gcccgttcgt atcgtgctct
    13441 ttcaaatact gattcacctt cgtgatcagt tgcgcgtcat gcgcccggtc cttgttcaac
    13501 cagaaaatcg ccggggcgcc ggtggcccgc gcgcgagcca cagctagttt cacccagtcg
    13561 cggatcgggg catccttcac ctggcacata cgccagatgt ccccttcttc gaccttgtgc
    13621 tccagcaagg cttttcccga cgcatcgaca acgcgaatcg taccgttgcc cggcgccttg
    13681 aaggtcttgt cgtgtgatcc gtactcttcc gccgcctgcg ccatgagtcc gacattgggc
    13741 acgctgccca tcgtcttcgg atcaagggcg ccgtgcttct tgcagaaatc gatcacctcg
    13801 cgatagaccg gggcatagct ggcatcggga atcacgcact tggtgtccgc cagcttcccg
    13861 tctggtcccc acatcttgcc gctgtcgcga atgaccggcg gcatggaggc atcgatgatg
    13921 atgtcgctcg gcacatggag gttggtgatc cccttgtcgg aattcaccat cgccatcggc
    13981 ggacgctgct tgtacacagc ctggatatca gcttcgatcg ccttgcgctg atcctctggt
    14041 aaggccttga tctttgcata gagatcgccg atcccgttgt ccggatccac gcccagcttc
    14101 ttgagcgtct caccatgctt ggcaaagaca tccttgtaat agaccgtcac agcatgaccg
    14161 aagatcttcg ggtccgagac cttcatcatg gtggctttca tgtgaagcga gaacagtacg
    14221 ccttgtttgg ccgagtcctg aatctgctct tcgaggaatg tgcgcaacgc cttgacgctc
    14281 atgaaggtgg catccaccac ttcgcccgcc tgcaaggcca ctttcggctt caggacggtc
    14341 gccttgccat cttggccgac aaactcgatc cgcacttcag tcgccgcggt cgtggtcagc
    14401 gacttttcat tcgaaaagaa atctccactc ttcatgtgac tgacgtgtga cttggaatct
    14461 gaactccagg cgcccatctt gtgcggatgc ttcctggtgt gcgccttcac ggacaagggc
    14521 gcccggcgat ccgagttgcc ttcgcgcaag accggattga ctgcactgcc tttgacctta
    14581 tcgtagcggg acttgatatc tttctctttg tcgtccttcg gattctccgg atactcgggt
    14641 agcttgtagc cttggctctg caattcctta atggctgcct gtaactgggg cagcgaggcg
    14701 ctgatattgg gcaacttgat gatattggct tccggtttct tggccagttc gcccaattct
    14761 gacagggcgt ctggttgccg ctgttccggt gtcagatgct ccggaaacac cgcaaggatt
    14821 cgaccggcca gcgagatatc ccgcagttcc accgatacac cggctgcctt gctgaaggca
    14881 ttgatgatcg gcaagagcga ataggtcgcg aacatcggcg cttcatcagt cttggtatat
    14941 atgatcttgt cagctttcgt agtcatgatt ctccctctta tcccccatga gttagttcag
    15001 cgcattcaac gctgaacccg ccttgaacca agcgatctgc tgctcagtga tgctgtggtt
    15061 cgtttggatg gtcagagctt tgccatccgc cttgtgtatg gtgatctgca caggtttgcc
    15121 tggagccaga ttgttgaggc ccgtcacgct gatgcggtct tgctgctcga tcttgtcgta
    15181 atccttcgga tctgcgaagg tcaacgccaa gatgccctgc ttcttgagat tggtctcatg
    15241 aatccgagca aagcttttcg tgatcactac cttcacattc aaaaatctcg gagacatagc
    15301 cgcatgttcc cgactgctgc cttcaccgta gttctcgtcg cctactacaa ctgaccctat
    15361 gcctttggac ttgtagtcgc gtgcaatctg agcaatcgtg agatttgcct cacccgtgaa
    15421 cacattcgtt cctctgcccg gctctgatgt aaaggcactg tttgcgccca aaaacatgtt
    15481 gtcgctgatc ttatcgagat gcccacggaa cttgagccat tgcccagctg gagaaatatg
    15541 gtcggtggtg gtctttcctt ttgtcttgat cagcagcggt agcttttcaa agtctttccc
    15601 atcccagcgt gggaatggtt gcaacagttg caagcgctcg ctggtaggcg gtatgtccac
    15661 cgtcaacccc tcaccgcttt cagcaggagc cacgaacccc tcttcacctt tcgcaaaacc
    15721 tttgactgga agttccgcac cttgtggcgg ctggaatttg aactccttgc cgtctgcacc
    15781 cttgagcatc tgattcaccg ggtcaaaacc aagatctccc gtaatggcat acgcggtcac
    15841 gacctcgggg ctcgccaaga acgagagtgt ttcgctgatt ccatcattgc gaccaggaaa
    15901 gtttcggttg aaggagctaa cgattgaatc ggcctttccc ttcaccccgt cggcccgctt
    15961 ccactgcccg atgcagggac cacaggaatt cgagaggacc gttcctccga gcttttcaaa
    16021 cgtatccaaa aacccgtcgc gcttcatcgt atgataaatg cgctcggatc caggagaaat
    16081 aagaaacgat gccttcgctt ttaaaccagc tttcaagccc tgctcagcca catgggcaga
    16141 gcggctgatg tcttcatagg aagaattggt gcagctaccg atcagtgccg ccttcagctc
    16201 gaccggatac cccttcgcct ttgcctccgc cgccaacttt gaaatcggcc gtgcaagatc
    16261 cggcgtatgc gggccaacta catgcggttc gagcgttgac agatcaacct caacgatctg
    16321 atcgtaatat ttctccggcg attgatagac ttccggatcc gccacaagca ggtctctgtg
    16381 agattgagca aaggtcgcca gatcagcgcg atccgtgatg gtcatgtagg cgaccatctt
    16441 ctcgtcgaag gggaacacag aggtcgtcgc cccaagctcc gcacccatgt tacagatagt
    16501 gcccttgcct gttgcactga tcgtttcggc ccctgggcca aagtactcca caatcttgtt
    16561 ggtcccgccc ttcactgtca gcagaccgca caggtagaga atgacgtctt tcggcgaagc
    16621 ccagccattc aattttccgg tcaggcgcac gccgatcaat ttcggatgaa gaacctccca
    16681 cggcaaacct gccatcactt cacccgcatc tgcgccgccg acaccgatcg ccagcccacc
    16741 caagccacca ccgttcggcg tatgtgagtc agttccaatg atcaaacagc cggggaacgc
    16801 atagttctcc aacaccactt gatggataat cccggctcct ggcttccaga acccaatgcc
    16861 gtacttcttt gccgcagaag cgaggaaatt gtagacctct cgattttcgt ccatcgcgcg
    16921 aagcaggtcc ttctcggaac ccatttcagc acgaatgaga tgatcgcaat ggatcgtgct
    16981 cggcacagcc gctttttcct tattggcctg catgaactgc agcatggcca tttgggccgt
    17041 ggcatcttgc atggccacac gatctggcct gagcgccaac atcgccttgc cccgctccca
    17101 ggtctgcgcg tcgaaattat ctgcgtgcga gaccaagatt ttgtcagcca gggtgaggcc
    17161 tcggccgaat tgctttctgg ccttggcaaa cacttccggc atcttggcat acagcttctt
    17221 ggcgagatcc atcgacatga cttatctcct taaaatatgt ctgtcaccaa atcagcacca
    17281 tcagccggcc gggaacatga cattccaacg accggattga cgagtcatat ttgacgaccg
    17341 accattgtcg tcttacttca acggcggaac ttcccgccgc ttcggtgccg cataattcac
    17401 aaggtagtcg aataaacgga tcagccggct atcctggcgt ttttgatcaa cccagtggcc
    17461 gatcaatccg atcgtccgcg agagaatgaa gaacccgttc aagctatcga cagggaatcc
    17521 gatatcgacc agtacggccg ccatggtccc atcgacgttc agaatcagat tatccttttt
    17581 cacggaggta attttctcca cctccagtgc gaagtccagg catggtgtct taatattcaa
    17641 gctcttcacg tacccaacca attccttcac tcgcttgtcc gggttgcgca aactcttcac
    17701 gcgatgacca attcctggaa ccggcccatg gtttttcttc atgtaggcca agaattcgtc
    17761 gaccgacatc ttgttatcga cagcatactt aaagaaccgc ccggcatcag taaccgcacc
    17821 gccgaagcgc ggaccaatca taatcatgcc cgcggcaact gattgcgaca gcccaatccc
    17881 ggcacaagcc gcaaggattg tcgcgtatgc tccgctcacg caagggccgt gatcagcgga
    17941 aagcatcatg atgcgcttaa tgatttcggc ttcctgtttc gagatcagcc gcttgtccca
    18001 caaaagaccg atgacatgag gaatttcgta gcctttgttg atgagctcag aggcaggata
    18061 accgtcgtag cagggctcat cgccgcggtc atcgctgatg gtcgtacgga tcagcggcga
    18121 gaccatgacc tcgtcagcct tcatcgcctc ttcgatggtt tttggcaacc ggggcaacgt
    18181 aaccggctcg accggttcct tgatctggcc ggacttcaag agatcttgat aggtttcctt
    18241 gatggcagga ccaagggcgc caaaagtggg cggcacgagg gcgccggctt tcttcagcgc
    18301 atcggacttg gagcgagctg atccttcacc cttcatcccc tctttcgcgc cggcatgacc
    18361 gaacttcatc cccttcggca aactctcctg acaaaatccg gagaccacgg caatcaactt
    18421 cacccggcgc tttttcgcgc cataccattc tgcagcccgc tcttcgagat cgccgcccat
    18481 ctctccgaca ataacgaccg ccttcgtttg gggatcgttc tcgaacatct cgagataact
    18541 gacgtaatcg gttccaggat aggcatcacc gccgataccg atagccgtcg tgatcccgtc
    18601 ggcaaactga gagcagatcc aaatgatttc gttcgagaga ccgccggact tggtgatgac
    18661 accgaacgat ccttcacgat aaagcttgga tagaacaaga ttgtcgaacg caccgccaat
    18721 tacgccaagc cggcacgctc cagcagagat gataccaatg gatgaggggc cgttaaagac
    18781 cttccccaac ttgcgcgcat gagcgccaag aatcttggca tctttttccg gcaccccctc
    18841 ggtgatcatt gagaccacct tgatgtgaga atcgtccagc gcctccatac cacccttcat
    18901 cgcgcggtct gcgcctatgt atacgagact ggtattcagg gtcggatgat gcttggtggc
    18961 ttcagcaatg gacttgtaga ccggaatggc gatcaggccg ctgccgtatg ggatctcatt
    19021 gctcttgccg gcatcgggtg gatagacaaa cgcctcaaca ttgagcggac gcttaatgag
    19081 ataacagaac tcggccatgc ggcgggcagc gttgacaccg gcttgaccac cctggatcac
    19141 cacacgggtg tctttgtttg ccagaatact catctcggga tctcccctta tcttcggttg
    19201 gtcataggcg gcaggctact tcgcttggag tgccttgtcg acaatgtcgg tgagcggtgt
    19261 attgcggtcg aagacattga tgtcaaagcc ttcctctttc aacgcgcgca tcgcatcaag
    19321 cccctctttt tcccgcggcc caccgcgacg cacccaaatt tttacgttct tcaacttccc
    19381 ttcggacttc gccttccgga agccgttaat gatccctccg aacgtcttct tcacgtcggt
    19441 gaaattggcg atcgcaccac caacaatgat gttccggatt cccggcagag aacagacctt
    19501 ttccgtgagg acctcgacgg cccaatcagg tggatcaccg gagtattcgg cataattggc
    19561 caacttgccg ccacgcgcca caaccgcatc tgagtagtac acgctggcac caccgcccgc
    19621 cggcaacatc gctgtatccc caccaggaat ttcgataaat ttaactgacc ccttaatttt
    19681 gctgtcgaca gccatcacct caagctcttg cttggaataa gcacggccaa attccgccgc
    19741 aaaagcgaaa ttccagtccg gatgtctaaa tttggcatcg ccatcgagca atgtcacggc
    19801 atcaagcgcc accagctctt catcggcagc acgaaggacg accggattca cttcgagata
    19861 ttgcgcatct tcactgtcga agcaggcaaa cattttccct gcaaacgcag ccatcttctt
    19921 cgcgagcggt ccactaaatc cggcctcttt cgacagtttt tcaagtgatt caggagaagg
    19981 agcttgtccg atctcaagac acaaccgctt aacacgttcc caatttgact caacctcgat
    20041 tccaccacaa ttggcaacca agatctcact gccttcgcgc gtggatttca ccgcgcaata
    20101 atattcttcc ttgtgcggga tcatctcaga gacaatcacc tgagttacgg tgatgctgcc
    20161 gacttgacgg ccgatcatct ctttagtggc ggcttctgcc gccttaaagt ccagatcgat
    20221 ctttaccagc cccaacttga agcgcgatcc gagagcctcg tgcgccttcg caacaagctt
    20281 ggacttcttc aaccactcat tggcttgacc cagcctggtc aactcgtcaa cggaagtgac
    20341 gaccacataa ttgggaacat taattcccca cttcttcatc agccccatcc cgggaccttc
    20401 cagcactttc gccatgattg acgactcctt ggtgtgtgat agggcagaga aacggagcgg
    20461 tagagtaccg aattctgctg attagctcaa tcctccaaaa ggcccaagct ttgagcacag
    20521 aagtcctacc ataatgagca ccgaagattc atcaccataa ataattgata acgttattat
    20581 tgtctccgat gcttcaaggc ttgcggttat cgagactttt caggccttgt ggcacgccga
    20641 cgcccgtctc gctggcatgt gggacagtaa aacgaacttc gttcgccgac ggaacgacag
    20701 atgaccgtac cgcaggcatt cgggcagggc tccccagcct tggcatacac cagatgcctg
    20761 ctcttgtact tgccttcggt cccatccggc gcaaaataat cccgcacact cgaccctccc
    20821 atggcaatag cttcccgtaa cacctcgccc atgacttgat gcaaccgcac gatcgacttc
    20881 cctggaatcg tattcgccgg ctgatcggga tggaggcagg aacggaagag gatctcattt
    20941 gcatagatgt taccgatccc tgcgatgacc tgttgatgca tcaggagaga cttcagccga
    21001 ctccgtgtcg cccggagcac acacaaaaat tgctcatgac tgatcgtgag ggggtcatag
    21061 ccgaaccggc gagcgacata tcgatccagc cccgcctggt caagcaacga taaacgacca
    21121 aaccggcgcg gattccaata tctgatatca ggctcagaac aaccatcgag gtgcaggata
    21181 aagtgggtat ggtggggatg cttggtcgag gtggatcgga acagcaataa tccggtcata
    21241 cctaactctg caacgagaaa tctcggctcc ttgccgcaga gaaagtgaag aatcacgctt
    21301 ttccccttcc gctcgacccc tgaaatcttg gcctgacgat actgttccag agaaggcaac
    21361 ccttctcgca cgatgtcctc acggccaatc cagcaatcac gaactgtggc tccgacgacc
    21421 cgttcgcgca attgacgcgc ggccacttcg gcttccggca actcaggcat caaatattct
    21481 ctcgatggcg gagtggactg tatggagggc aaattacgcc atggggccga cttaaaatgc
    21541 aactctgacc tgagatagtt ccgcaggcat cactttggat aactataacg cgaccggccg
    21601 gaggggggag gaggaactga caagtaggac aaatcggaat gtcccgaaca gcgtaacttt
    21661 tagggcgttg atgaattcac tcgagcttgc cgccggcgac aaacatcctg aatcttctct
    21721 tggaggcgcg gcaacggcat gccgcctggt cgcctagtca acacaagctt caccgcatcg
    21781 tcataagcca ttccttcgat acagcataga taggcaatta ccactgacac cgatcgcccc
    21841 atgccgaccc ggcaacagac cagcaatcga ttgtcagcca catgtgccgc aatccattcc
    21901 acagcctcat acaacgcaga cgcggccggc tcaccaaact ccttgagcgg aatcttggca
    21961 tagataaccc gcgagggaac tgtcacattt tgttcttcag ccaccatgag aacggcactt
    22021 acctgggggg gaggattcct agcgtcatcg gcattgccga ccagaagacg tttgttgatc
    22081 atgtgcatcg tgtcgttagt atagggggca tagtctcccg gtcaagtgaa tcctcgaaat
    22141 tcgggtaagg cggttgaaca tcgctgaaac cggtgcgtat actcaactcc gctattcgag
    22201 gaggaattga tggctcacac gtcgcttgaa gcggtagaaa aacggctggc gagtgtccag
    22261 tgcgcaatct gtaagaccaa taccttcggc gtcgaccggc gcacattgca gccagatgga
    22321 gaatgccggg cggtctgctt gaaatgtcgg tacaattttc ccgtctacac agacatggag
    22381 ttttaccttc ggactcaacc agatgtgccc tatcgcctga aagagatctc ttgcccaagc
    22441 tgccagcatc gaggcgtcag tctcgatttt cgcatcacca tgtccgtccg cgaggcgatc
    22501 tacttcgtga cctgcagcgc ctgcaagaca cagtttcctg agcgctcgtc attggaagcg
    22561 tttgagtagc ccttacgaac gcaatttcgt gtatactgag tttattatga gcaccacacc
    22621 aactccagat cccccgaaag cccctactcc ggtcactcag actgaaacta agccccgcaa
    22681 ggaaattcct ctcatttcag tccctaacga tcgggacatg attgcggcgg aaatggccga
    22741 actcttcgat gaagatcggg tgtcccatca gcacggctcc tcagaacccg aagctgacta
    22801 gatcttcacg caggacgaac aatctccact acgccggacg agataatccg gcccaccatg
    22861 gccggtcgcc cttttcgctc ctttctgaca atatcgctca cccgaatgat ggccccgcga
    22921 tgccagtcat cgaaggccgc gttcgaactt gagcgttcct tgtcctggcg cccaatctcg
    22981 gacctccccg catgatcgca caaccagacc cgtttctcgc ccttcatcac acgcaactca
    23041 ctcaccacgc ggtggtagtc cggatctcgt ggcacgagcg tttttccgtc cttgcgaaaa
    23101 atcacatagg aaaatttgag tgcatctttg agaagcccga cttcccgatc aacttgagca
    23161 atccaggctg gagtctccca cttccgttct tcatggcacc agtcatccgg tttgagtagt
    23221 gcaggacaag gagcgtcatg gagacagggc gcaaagacag aacagtactg ccctgccaga
    23281 aggttgtccc gcacctgatg caattcgcgc gaagcaggcc gcgtggccgg ctcgataagc
    23341 attagactcc cttcaggggc caagtaattc aggagttccc ctaccaacgc agtccgttgt
    23401 cccacgggat ccatactccc tacgaataac tctgagagaa gattctgaac aattataagc
    23461 tggtaaccat tcacaccacc gccgtcacgg atggcggagg gcagggaacg ttcgaggtta
    23521 cactccacgg tgttcaacgg tgggatggac tgatcccgct gctgttcaga atacgcctgc
    23581 caaatctcag tagacaccct taagacctga ggtgaacgat caacggctat catacggagg
    23641 gtagacggcg gcctcgcaga ctttgaaagg caccaatcga gcactcccag gactcctgtc
    23701 ccaggcccgc caccgaggtc caatactcga aaatcatggt cctgagcgac gggaagcacc
    23761 ggctgcagtt cgtccaagag catctgcact ttggcgagat taacaggcag gtagtagacc
    23821 aagtaaccgg cacgcaactg agggtcctcg aagtaaaccc tgcttcccac ctgcccattt
    23881 cgtgtaaaca tttctgaaag tcccgacacc gcctcaatta ttgattggga tggcccctca
    23941 gagtgctcaa ggagatttac tttggccacc ttcgtgatag ccctcaaaac tgccgatgat
    24001 agttggtaca tagacggacc ctcggttgac ccccccaagc aggctggggt attctcatga
    24061 gagaaggagc acttcacatg actgacccta ttttgcaagc atatctggag gtggagatgg
    24121 ccatggagcg cttcacgctt gtcctgcatg accatgtcga tcatctccgc aagactgaag
    24181 cccctggctc agacaagctg caccgtatgg ccaacggcac caaagccatg cgcgatagcg
    24241 catcgattta cctatcctac gccaagtacg ttgctcacgg gatgccggaa agccctgatc
    24301 tagtcgagga ggatcttcag ggctgaggag ccgcactcaa gcatctcccc aaatgcacag
    24361 ggcccgttcg gtatgaccga acgggccctg ctgtttctac cctgacgtta cttctcggcc
    24421 taaatgctgc gcatgaaatg ccccatggcc tcatgatcag cagctgatgc gcccataact
    24481 tgtgaaatcg caacgttcgt ggacttcttt ccggtcagac cggactcgac ttcgtccacg
    24541 atcaattcca ccggcatgga ctgcccaccg tacactctcg ggccaccgat aatcttggca
    24601 tttgagaaac cgtaaatagc ggttgccact tcttttgcta accatccgac atagttgaat
    24661 tcaggcacaa caattaattt ggccttcccg cagagtttgc gcaactcctg agtcgggaat
    24721 gggcggagcg atcggatctt gatcagtccg atcttaatgc ctttttcggc gcaaatacga
    24781 accgcttcac gtgattgagc ggccgcacta cctgaagcga taataaccgc ctccgcatca
    24841 tccacattct ccgccgtgag cagtccaccc atgtactggt tgatgtattt acgggaacgc
    24901 tccacagcag cccacacttc ctgctgccat accgcgtgaa tgttgtaggc catgaaattg
    24961 gacttctgaa cgggagcatc gcgagagagt cgtgccggag gattctcagc atcaagcacc
    25021 ggcaccgccc cacgccaagg ttcgcgcggg ggaagcttca tcccacgatc ttgcatacgc
    25081 acatagccac gggcatgcgt cacaaaaaac ccatcgcagc acacgccgac ggggagggtc
    25141 acatcattct tctcgctgat aataaaccca gccaacgtaa agtcgtacat gtcctgctga
    25201 ttttcggcat ggaacacaat catgccgcaa ttgagcagat aagaaacttc gatattgtca
    25261 ggttgaatgg ccaacggtgc gttcactact cggcaagtga acatggccac gactggcaac
    25321 cgatgccccg gccaagaagc aatcccttcc agccctctca gcgtccctgg acccgccgtt
    25381 gcagtataac accgcactcc cgcccgtgac ccgccggcga tagccgccat gacgccgacc
    25441 tcttcttcgc cacggtagta ctccttgacg tacccttcac catacaacac accgaccaac
    25501 tgcatagttt cactttgagg ggtaatgggg tacgcaatgg caagatcgac gtttgagcgg
    25561 cgaatagcct cttttgcggc ctcgctgcca gtgatgaact ccttcgtgcg aggagcttcc
    25621 aggaacatat actcaggagt gacaactttc tgtcgctttg catccgcgtg cggatccttc
    25681 ttggtctcgg ccttcggagc cgacacactc gtagtagggt cgctttgtgg attggtcttt
    25741 tgtttgggtt ccaatgcttc gctcatcgtt gcacctcttg actgtatcgc cgtagcctaa
    25801 aaccttagcc ttctcgcttc atctgctcag ctgagtggcc aaatgtagcc gcgctgccta
    25861 cgcctgcagc ggaaggcata aaggtcatag gattcgtatg cgtcttccca ttatggacct
    25921 tagactgagt tccggtgaat cgcacgttct cgccattatc cggcaactta atgcccattt
    25981 cctcacggac cttcttgaag atttgcgctg ttctcttcaa tttcaacgtg tccgaatcgc
    26041 gaattgtcag catggcgtcc tcatgcccct gctcagcaca aatggccatg gtgcagcagt
    26101 tcgtccctgc acgaatcatt ttcaacgtaa gattggcata gtgacagtga acgccgatga
    26161 aaatacaggc ctcaatcttg ttgccccaga tcgtgagatt ggggtgattg ggattgatga
    26221 cctcttcagg gtcaattttg gggtacttgg ggcgataatc cggcatggga ataatcatca
    26281 cctcagggat ctgtgccgca atttccaata cagccttcgc cttctcgaca gcatggttgt
    26341 tccaagccca caagacgaga ggtcccggaa aaatcgttgc atttgggctt gtcagcattt
    26401 tccgagccat ctcttcaatc actatgtctt cgttaggttc cagcagccca tagtacagcc
    26461 cttcgcctgg atcaggcagc gcgaccccta attgagctgc cgatggagga tggaatccgg
    26521 ctggccccgg cacgataatt ctctctcttg tatcttgcgt cattcccacg cccactcccc
    26581 tttcttacgc ttacccgact actgggctcg ccaagaccgc tgtcgtactc ttatcgctat
    26641 atgtgatgtt atcggtcaac gcggccatcg gtaattgatc aatcatgatc atcttaatgg
    26701 cattgttttt cgccatgttg tcgcagaccc acacacattg tgcgcatcct ttacaacgat
    26761 ctacggccac gtatgcggac ccgtacttaa agcccttctc tttccccatc tcctcgctat
    26821 actggatggt attagcctca ggacaatact gagtacagag cttacagctc ttcgaggcac
    26881 aaatttcaac atcgatatgg gcgactaaat acattggaac tccttcgtac taaatcctgg
    26941 ttaggcgctc acagtcgcac ggatttccga ccgtttgacc gtcggtgcag cccaaccgtg
    27001 gtcgactgca tagttccagc cggcctgaat aaccgcgaca ttcttgtcga tcaactcctg
    27061 cttcttttta aattttcgct cgacgacact gtccagggcc gcggttcctc cggagaccac
    27121 aaatccttta ccaaggaacc gatcctttac cgactgctcc agcgcttcaa cagtagtcaa
    27181 cccggtgatc gctccgatgc aacccatcaa ggccatattc gtgcacaagt ccatgcccgc
    27241 cacttcgagg gatagcttag tagcgggaaa atagtacagc cttgcacgac gctcttccaa
    27301 ctcgcgagcc tgatcccgat gaagcttcat cggaccatca ttattgatca acgcgattcc
    27361 gtcttctttc aatccgaagt aaaacggcat cgtgtacgat tttccgtgag taatgacctg
    27421 tggatggaaa atgataatga tgtgcgggaa ggtgatctct ccgatttcgt agatgggctc
    27481 gtctgatact cgcacataac tctccaccgg agccatgcgc ttttctgatc cgtagaacgg
    27541 aactatcgta ctttccccac cggcattaat caccgcagta ctcaggatgt gcgagccggt
    27601 cacaaccccc tggccaccca cacccgccat ccgaatgttg aaacgtttcg ccatgctcaa
    27661 tcctccttaa acgttgctca ggctttcgga agcccaggag cgggagcagc cggcttcggg
    27721 agaaattcct tgtagccata ccgttccgta agaaattgct tcgcctcgtc ggtaatatat
    27781 tcggagaact gatagcgatc gttttcaacg ttcttcgcgt cttccatgac cttgtcggtc
    27841 ggaatggcat actcaatgtt gcaagaagta taggcctgaa tgtaggtcga cccaacctcc
    27901 ctggcgatca acacggcttt tttaatgacg ctctcaacac ggcgaggatt gttcggcacc
    27961 accgtcgcga cgtaggcgca gcctgcaacc ttcgccatct gcagcatatc catcttctca
    28021 aacttcttcc ccaacggagc catcttgagc acggcgccgc gattcgtcat gccgctctcc
    28081 tgcccgccag tgttcccata cacttcattg tctagcatga tcgtggtgaa ccgttcttta
    28141 cggaaccagg agtggaggac ctgctgaaat ccaatatcag ccgtaccgcc gtcacccgcc
    28201 atgacgacca catccttcgg cttatcgcca aaacgaaggc gcaagccacg ggacaacccg
    28261 ctagccacac cattctggtc accatagttg ccgtagacga aggggatggc agcttgcgaa
    28321 atcgccaagc gcccgcaacc ggctgttcca acagtgatag tatcttctgg attggggaag
    28381 gcaatcatgg cgagtcggat aaagagggtc atcgcacaac cggcacacat ggggtgctct
    28441 tcaagcactt ctttgaagga gcccatctgt gaaacggaca cctttttgcc gaagggacca
    28501 tgctcaacca tgtcccgata ttctttcggc atgaactttt caaagccgct tgaaaacttg
    28561 acgtaatcaa ggctcataag gactccctcc cttttataag ctgaggactt tgttcggacc
    28621 aacaacagcg gccagaaaaa gccaggtgca gattatcttg acgtcatatt ggactaagta
    28681 ccgaactgtg ctcctgacac ggaacggaat gttagtcttc ttacttcgtg tagtcgccga
    28741 gtgtaacaaa gcccgaaaaa atctgtcaat cgcaaccagc acggaaaaag caaaagatcc
    28801 catcacttca ggacaatggc gagtctatag agtccacaaa agcattgcaa ctttgcagaa
    28861 ggcccacacc ggacgaaaga aggcctagta gagatacccg tgctaggccc ttggccctaa
    28921 atccgcttga acgcgaagat tttactcctc tgcaagtccg atctgtcacg caattcagtt
    28981 gcagttcacc cgagaagcgg gtaaacattg ttgcttaacg cgtttcggtc tacactccgc
    29041 ctatgcctat acgagtactg gtcccgggag aagaacatgg cgagcagcat gcgggagggg
    29101 tgcacaagcg accaccaatc ccggatggct cgctcaaggc cgagtgccca aaattcatga
    29161 gccatggccc ctgcggaggc gtccgcaaag gtggattctg cgaggtctac ccggaaatga
    29221 cctgtccgtg ggtcacgctc tataataaat tgaatgaact gggccagctg gagtggatga
    29281 agcaagtctg acaacaagaa ggtggagggg agcgggtgac ggcgaaaggt agtaaaaagc
    29341 ggaagccagc tggggcaacg acaaagctgg gatacaacga gcggcacgcc aagagtggaa
    29401 tctctgctcc tctacccgac attgagacat ttcccaatca atataaggga tacgaaatta
    29461 caatcgtcat cccagagtac acagccattt gccccaagac caacctgccg gacttcggaa
    29521 ctatcaccct tcactacaaa ccagacaagc attgcctcga gctgaaagct ctcaaaacgt
    29581 acatccatgc ctacagaaat gtaggaattt tctacgagaa tgccgtcaac cgcatcctcc
    29641 aggacatcgt acgagcctgt cgacccatca atgccacggt aaccggtgaa tttgctgctc
    29701 gtgggggact gcgtagtgta atcgaagcaa aatatccggc ataaattgcc cactccacaa
    29761 ccttctctgt acgacatcca ctggctactt atcccgtttc acctcatcac caacacaccc
    29821 atcgcaacta tcccagtaac actgcggcag cttcctacgt gctgcgcact gccaatgttt
    29881 ctcactaaaa ccgcctaaag tcccactcaa aatccaccga catcagacag tcagggtgcg
    29941 gcatggggcg gcacaggcta cccgtacgtc gcaacagggt cgctgccaag caggaaaagg
    30001 acgccgtctt gcgcacgcgc tccatgggag cgaccatcgt tcaggcgatg gtaggactct
    30061 tcgttgtgat atcgggaacg tacgacatgt cggttcaaac cgcagaagct atccccgcct
    30121 ttgcgcgaaa atacacgttg aactgcacga cgtgccacac ggctccactg cagatcaata
    30181 cattcggtga acgtttcctt gaaaacggct atcagcttcc gggaaccgaa gatgggggcg
    30241 tcacgggaaa gaagaatctc agcgcaccgc gactggccaa cctgtacgcg caaatcgaag
    30301 cggcatgccg ggtaggcact ccggacaacc ttcccgatct ctgcgggcat ctgattcctg
    30361 aacaccgggc ggtatgcgcc ctgatgcgtg aagaactcaa ccgggcagga cggtcggccg
    30421 catgatgcac agtctgaagg acaagccgtg acgatgcgtc ggctcctgca gcgactccgg
    30481 cagagcgtgc ggcgcgctac ccggcaaccc gcgaccgacc cgggcgaccc gcaaaccgaa
    30541 cgccaggaga tccggccttc caacaaggct gagtcccttg acgaaggccg cgcactcgac
    30601 gccgtcctct gctcgctcga cgaaggcctg tgcatcgtcg atgcgcaatg gcacatcctc
    30661 agactgaatc cccaagccga gatcctcttc gccgccgccg cccatgacct gaagggacgc
    30721 cccgcttatc agctgattgc tcccggcccg gaagaattcc ggcacgagtg cctcgtaacc
    30781 gacagctcca gccctccact gaaatccgga cttccctacc ggaccgacaa tggtctcctg
    30841 ctcagacaag atggccaact gatgcccatt tctctgacag tcaccccgat gtcacacgat
    30901 ggcgtcgtga gcggaaccgt cctgacgttc cgggatctca cggcccagaa agaggcggac
    30961 tcacatcagg tcgaacactc cacgttgctc cgccggttac aggcaggcat ggtcggatta
    31021 gcgagcaacc aggcgatcta ccggggacaa ttgaccgagg ccttccacct catcacccgg
    31081 gtcacgacac agagcctgca cgtcgcgcgg gtcagcatct ggctttatgc gtcggatcat
    31141 gcggtcctcc ggtgcgcgga cctctatgag caaccgggca atcaacactc gaacggcatc
    31201 aagctacccg cggcccttta tccgcagtac ttctcatcgc tcgaggcggg agagatggtc
    31261 gtcgcggacg aggcgcagtc ggacccgcga acggttgaat ttgcggcaca acatctgctc
    31321 ccgctgggga tcacctccat gctgaacgtg cccatccgcg ccgagggcag tctggtcgga
    31381 gcgctgcact gtgagcacat cggagcgccc agagtctgga cgacggagga acagcagttt
    31441 gcggcctcag tggcgaacac cgtctcgctc gccgtggaag cggcggaccg gcataaggct
    31501 gagaccgaaa cgaaacatgc cgaacaattt ctcgactcgg taatcgaaaa cctacccatg
    31561 atggtctatg tgaaagaagc cgaacagctc cggttcacac gctggaatcg ggcggcggag
    31621 gcgctcacag gattcagtcg ggagctcatt ctcggcagga ccgatcatga ttttttccgc
    31681 aaggaggagg ccgaccgctt cacacgccat gatcgcgagg cactccggag cggccgcctc
    31741 atcgaggttg cttcagaaga aatgcacacg cagcaccggg gcgtgcgact gctgcgcacc
    31801 aagaaactgc ctgtcatgga tgagcagggg cgcccccgct tcctgctcgg catcgccgaa
    31861 gatatcaccg accggaccac gcacgaatcg agtctgcacg agagcgaaga gaagtaccgc
    31921 gagctgttcg agtcctcgcg cgatgccatc atgattttgt ctgcgccgaa ttggaatttc
    31981 acggcctgca acccggcgac cgtccagctc ttcggggcac gggacgcaga gcatttcacg
    32041 tcactcggac cgtgggacgt ctcacccgag catcaacctg acggcgaaga atcgatggtc
    32101 aaggcgccga agatgattgg gatagctctg caggaaggct ctcacaactt cgagtggttg
    32161 cacaaacgag tcggcggccc cgccttcctg gcgagcgtac agttgacccg catcacgttg
    32221 aaagggcccc ccggactgca ggcgaccgtc cgagacatta ccgaacaacg ccgcagcgaa
    32281 gccgcactgc aggcctattc gacattccag aaggccatgc tggacaacgc cggtcatgcg
    32341 atcatctcct gcaaacccga cggcatgatc caagtcttca atccggcggc ggaagccctg
    32401 ctgggataca acgccgacga gttggtcggc aaacagaccc cgggcatctt ccacgacttg
    32461 gacgaagtgg tcgcgcgggc acgtcaattt tccaaggaat taggcaccac gatcgaacca
    32521 ggcttcgacg tgtttgtcga aaaatgccgg aggaatctcc ccaacaagca cgagtgggcg
    32581 tacgtgtgta aggatggcac ccggaagacg gtgctgttga atgtcacggc actgcgggag
    32641 gcggatggcc ggatcaccgg ctacctcggc atcgcctccg atatcacgcc gcttaagttg
    32701 gcggcacagg agttggtttc ggccaaggaa gcggccgagg cagccaatgt ggcgaaatct
    32761 cgattccttg ccaacatgag ccacgaaatc cgcacgccac tgaacggcgt cctcggaatg
    32821 accgaactgc tgctgaacac cagcctgtcg gccaaacaac agtcgctgct ggaaaccgtc
    32881 cgccgttcgg gggtggccct actcgaagtc atcaatgaca ttctggattt ctcaaagatc
    32941 gaagcgggaa agcttgaact cgaacaggtg gagttcggcc tgagacaggt cgtcgaggag
    33001 gcagtcgaga tatttgccgg gtccgccggc aacaagaaac tggagttgac ctgcttcatc
    33061 cccgccgacg tgccggatag catagtggga gaccctgtac gcctgcggca aatcctgctc
    33121 aacctgatcg gcaacgccat caagttcacg acacagggag aggtggcagt ggcggttgcg
    33181 ctgacgaaac aacatgcgga cacgttcacg ctcgcttgcc aggtacgcga tacgggaatg
    33241 ggcattcctc acaccgctca ggcacaactg ttcacggcgt tcacccaggc cgacggctct
    33301 accacccggc gattcggcgg caccgggctc ggcctcgcga tcgtcaagca actggtacag
    33361 ctgatgggtg gagaggtcgg cctggtgagt gcacccggcc agggcacgac gttctcgttc
    33421 acattcaccg tcacacgtgg aagtgctcac gccgcatacc ctccgcttgc cgaacaccat
    33481 ctcgccgggc ggcagattct gatcgtcgat gacacgcgta cgaatctaga gattctggcc
    33541 acccacttgc gtaattgggg cgccacggtc tttaccgcgg agtctgccga cgacaccctc
    33601 ctccaattgg agcacttccg gcataggcag catcggatcg acatggccat cctcgacctt
    33661 cgaatgccgg accgagacgg cgtcgatctc gcgcgttcca tcaaacagca gacggcctat
    33721 cggagcctcc ccctgatcgc actcagttca gttgagggtc tctcggacga aagtgacgcg
    33781 acccaccagc tctttcacag cgtcctgcgc aagccggtgc gacagtcact gctcaaagac
    33841 tgcctgacgc aggtgctcgg aggctccgcc ggaacagtga cgccaagtcc gcaatcctca
    33901 gcggccccac gaccacgcgt cgaagcccac atcctcctgg ccgaggataa ccccgtcaac
    33961 caggatgtgg cgtcctccat gctggagatg ctgggatgtc gcatcacgat cgcacccaac
    34021 ggacgggccg ccgtcgaggc agccacaacc ggccggttcg acctcattct catggactgc
    34081 cagatgcccg agatggatgg tttcactgcc acagcaacca ttcgccggca agaagccaca
    34141 gcgaccgacc gccgccatgt gcccatcatt gcgttgaccg ccaatgccat ggaaggcgat
    34201 cgcgcccgct gcctggccgc cgggatggac gactatctca ccaaaccgtt taccgtcgcg
    34261 caactacacg ccttcctcac gcaatggttg acgccacaac cggtcagggc gagcgcgacc
    34321 gatcaggcct cctccaccta taccaccgac gccgacgaac cgactgccac agaggaagca
    34381 ccgattccga caaccatcga caagactgcc tgggatgcga ttctgtcgct ccaacgcccg
    34441 ggacggcccg acatcttagc ccgcgtgctg gcaacctact tgcatgattc ccgccagctg
    34501 gtggagcaga ttcgctccgc cgtgcagtca caggattcgg tcgctctttc tcaggccgcc
    34561 caccgcttga agtccagcag cgcccagctc ggcgtcctgg ccaccgccgc ccattgcaaa
    34621 gaattggagg cccttggacg gcttgcccgg atcgacgagg ctgcgcacct tctgtcgcaa
    34681 ctcatcgaag cccatcagtt cgcctgcgcc gcgatcacct cggaactgca acagcgctcc
    34741 gcccgataaa ccaaccgttc gaccggctct cgcgacttgc gaatggacgt cgtccgcgct
    34801 agtctgcacg cacccatgcc tacctatgca atcggcgatg tgcaaggctg cgttgcctcg
    34861 ctcagacacc tcacacagac gattggattc aatccggcgc aggatcgtct ctggttcgta
    34921 ggggatctcg tcaatcgcgg gccggattcc ctcgccgttc tgcgttatat tcgtgggctc
    34981 ggaccggcag cggtcaccgt actcggcaat cacgacctgt tcctacttgc cgtggcgacc
    35041 ggcctggcga cccttcgccg cgacgatacg ctgacccaag tcctggacgc cccggactgc
    35101 ggcgaactca cgacctggct cagacagcaa ccgttgctct atcgggaagg ttcctatcta
    35161 ctggtccatg ccggtctctt gccgcactgg acgatcgagg aggcccagca attcgccatg
    35221 gaggccgaaa cggcactgcg tggagaacaa ttcagtgcaa cgctccgcgc actccacccc
    35281 agcggccctc tgcaatgggc ccctgatctg aaaggcccgg tacgccttgc caccatcatc
    35341 aaggtgttga cccgactccg aacctgctcc gacaacggca tcatggaatc aaccttctcc
    35401 ggcccgccga aactcacacc ccagggattt cacccctggt tcgatgtccc gaaccgacga
    35461 agtgcgaccg ccacgattgt gttcgggcat tgggcggcga tgggtcttca tctcacaccg
    35521 aacctgctcg gactggacag cggctgcgtg tatgggcgac agctcaccgc ggtacgactg
    35581 gaggaccgga aggtcttcca agtgcgttgc gaggagacgc gtggaggcaa gtgagccgcc
    35641 ggtcacggtc gattggcccc gctacagtag cagggcgctg cccacctacc gattcctccc
    35701 gggcctgact ccgcatccac accgcgatcc actggggcat tccttcgcag agcgggagcc
    35761 gcgcccgcgc ccgtttgaac cgcaccaatg gtcgaactcg gaggactatc tgtatgcgat
    35821 cgatctctac aactttttct attggtggga agcgcacgaa gtgttcgagg gtttatggca
    35881 cgcgtgcgga cgcaagacta cgacgggcaa tttcttccaa gccctcattc agttcgccgc
    35941 cgcccatctc aaacgtgcac tcggcaatga aatagccgcg cacaaccttg cccgtagcgg
    36001 gctcctgcgc ctgcagaaca gtcccactcc ctacatgggg cttgataccg tcacattcgc
    36061 cgatgacgtc acagtgtgga tgggggacaa cagccggcca ggcgtgcgga ttcgattgag
    36121 acactatggc aacgggccat cagtgaacac gatcccctga gcaatccatc gctcctggta
    36181 ggggcaggaa caccttaggt tgttcaaaca ggtcagccga caagtccgcg tggagaagta
    36241 cgcagcaccg tcgctgctgg cgacgttacg gattgattcg agccgagatc gaagttgggg
    36301 gggagcggca accggctagt gataactttc agaatcggat gggaagctgc cctgctcaac
    36361 ctcatccttg tagcggcgaa gcgcttgcac ggcatcgacc ttgagatggg caaagggttt
    36421 gacgaacttc ggcacgaact catcgaatag cccgagcagg tcatacagca ccagcacctg
    36481 accgtcgcaa tgcgggccgg ccccgatgcc gatcgtcgga atcgtgagcg cctccgtcac
    36541 ggcctttgcc aaccccgcgg gaatcgcttc cagcacgatc cccacagccc cggcagcttc
    36601 caacgcctgc gcgtcctcaa gcagttgctg cgcccgatcc ttgccctttc cctgaacctt
    36661 atacccgccg tactgatgga ccgattgggg cgtcatcccg aggtgcccca tcaccgggat
    36721 accgatcttc acgatcgccg acacacggtc cgcgaccgca gccccccctt ccaacttcac
    36781 ggcatgggct ccggcctgaa tgaaccggcc ggcgtttcga agcgcctcct cctggctgac
    36841 ctggtaagac agaaagggca tgtcgccgat aaccaacgac cgctgcgccg ctccggccac
    36901 cagtttggta tggtacagca tctcctccat cgtgactaag agggtattcg acttgccctg
    36961 taccaccaca cccagggaat cgcccaccag gatcacgtcg atgcccgctt cttcgacgat
    37021 gcgcgcaaac aacgcgtcgt acgcggtgac gaccgtgatc ttccgtctgt cgcgcttgca
    37081 cttctgcaag tccgggacgg tcatcacccg agctctgctt tcgtgagaat ttccggcagc
    37141 cgctccgtcg acttgatcgc catgatgtgc acgccgtcgc agtgcggccg tacggctttg
    37201 atggtgcgca cggcaatctc gacaccggca tcgagggccc gcttgtcccc caccgcgcgc
    37261 atttcgtcga tcatctcctg cggcacacag acgccgggga tgttggcgtt cataaattca
    37321 gccatcttgg catttcgcag caagagaatc ccggccagca ccttgacctt gaatggacgc
    37381 accgcctcca tgaatttctt gaactgctcc ggctgataaa tcgcctgggt ttgaaagaac
    37441 tccgcgcccg cccgcacttt gacctcgaac ttgaccaaca tcgggccgag cggatcggct
    37501 tcgggagtca cggccccgcc gatcgtaaag gcggtggacc cgtcgagctt attgcccgca
    37561 tagtccttcc cgttattcaa tcccgtcacc aactgcatga cttgaacaga atccaggtca
    37621 tacaccggct tggcttcttt atggtcgccg acggtgggat agtctccggt cagacagagg
    37681 atattccgga tgcctagaat gtgcgcaccc atgagatcgg attgcatcgc gatacgattg
    37741 cgatcacgac aggtcagctg catcaccggg tcatgtccca actcatacaa cacacggcag
    37801 accggcaagg acccggcgcg tacgatcgcg gccgtgttgt cggtgacatt cactccgtgc
    37861 acgcgcccga cgagcgcctt ggcactctcg accacatggg tcaggttcgt gcccttcggc
    37921 gggttatact ccaccgtcac ggcaaattgt ccctgggcca atacgtcttt gagtcgccgc
    37981 ggctctcggg tcatgcgagt ctcctataca tctcgatcct tacctgccct tctccacggt
    38041 cgcaacggac aggccgttgg ctccatactg cgcacgcctc gcgagggcct tccggggccg
    38101 cgcgtgatgc gatgcgagga atatcgaggt cccatcggcg ccggcgagca aatgagccgg
    38161 cagcccctcg atcgacccta tttcatccct gccattctct tagcagattc aaccgtattg
    38221 tcgagcaaca tcgcaatcgt catcggcccc actccgccgg gaaccgggct gatccacccg
    38281 gccttctggc tgaccggctc gaaatccacg tcgccgcaga gcttgccgtc cggcagcttg
    38341 ttggtgccca cgtcgatcac gaccgcaccc tctcgcacca tctccgccgt cacgaacttg
    38401 gccttcccga tcgccaccaa cagcagatcg gcctcacggc agaccgccgc aaggtccttc
    38461 gtcttcgaat ggcaaatcgt aacggtggca ttgcgttgga gcaacatcag cgccaacggt
    38521 ttgccgacga tattgctccg tccgacgacc acggcgcgct tgccttcgat cgacacgcca
    38581 gccgattcga tcatcttgat gacccccttc ggcgtacagg cttcgaagac cgggttgcct
    38641 tcgaccagcc gtccgaagtt gtaggggtga aacccgtcgg catccttatt cggcgagacc
    38701 gcctccagta ccacgcggct ctcgatgtgc ttgggcagcg gcagctggac caagatgccg
    38761 tggatcttcg gatcggcgtt cttcttctcg atcaatgcca gtagctctgc ttgcgtcgtg
    38821 ctagccggca acttgtgatc gtccacatag atccctgcca attcgcaggc cttctgttta
    38881 ttcctgacgt acacgtgcga cgcaggatcg tctcccacca aaatcgtcgc cagtcccggc
    38941 ttgactcctg ttttggccag caccgcggcc gactctacag ccagtcgttc ccggacttgc
    39001 tgtgccaacg cttttccatc gataattcga gctgccacga tatcctccct ggcctgaata
    39061 gaatcaaaaa gtttcggcac catagcaggc aatttttcaa caagtcaacg ctggcggctc
    39121 cgcgtagacc accgacaacg ccccgttagg aagcgatatt ccaccgctcc gctcctgtcg
    39181 cagccacttc gcaatcagtc gcgcctccat gcaccgacct gcggcttcct tgacagtctc
    39241 cgaggccctt ggctacgatg catctacaga atcctctgtg cgacaccttc gttcatgaat
    39301 tccaggacca tccgccacaa cagattcaca cggctactgc gccgccgccg tctcggtctg
    39361 gcgctcgctc tggccattgg aatcctgctc catcacccct gggcctgggc gctccaagtg
    39421 agcggactgg agtctccgca tagttttctg gccgacccgg cgacgaggtc ctattacatt
    39481 tcgaacatca acggggaagc cgatgcccgg gataacaatg gattcattac caagctgagc
    39541 gacgacggcc ggatcaccgc cttcaagttc attgagggcg gccgtggaga cgtcacgctc
    39601 catgccccca agggcatggt ggtcgtcgat gacgtcctct acgtcaccga cctggatacc
    39661 ctgcgcgcct tcgacaagac gacgagtaaa cctctcgcta ctctgacgct cccacgcccc
    39721 gcagccggcg gcgccgccca gtccctcacc gatgtggcct ccgatggcca aggccatctc
    39781 tacctagccg accagggcgg caacgcgatc taccgcgtgg atctcgcgcc gacattgacg
    39841 ttgtcgttgt atgtctccgg tgcgcacttg gccggcccct ccggaatcgc cgtgcatccc
    39901 aagaccggac atgtggtggt ggtgagttat gattccggaa agattttcga catcaccccg
    39961 gaaggtgcgc tcacagaact ggcgtcgaac ggatttttta ccgcccggtt ccagaatctc
    40021 agcggcgtgg atttcgaccg gtggggtagc atgtatgtct cggatgggac gaagggcaag
    40081 atctggcgca tgctcccgaa tggaaagttt caagtgatcg cagaatacct gccgggtcca
    40141 tccgatgtgg gaatcgatcg cgtcaaccac ctgattctcg tcccctacca ggactccaat
    40201 gccgccgagg tcaacggcct cgaatcgccg acggctgcat cgggtgatcg agccaaacgc
    40261 acactcgcgg attacggatt cgtcgaaccc aagaaatccg acaaggaagg atcccctcgc
    40321 aaatgagcca atgtgtctac tgcaagcaac gcaagggcaa acgctcctgc cccgccctca
    40381 ccggtttgat ctgcagccaa tgctgcgggg aacatcgact gacgagaatc tcctgtcctc
    40441 ctgactgcga ctatctcgac accgggagcg actaccagca gaagcggctg ggcgagcagt
    40501 ttgccccggt ccggcgggag ctctaccggc aactgagtgt ggcgggaggc gaaaaggcgg
    40561 ccgcgctgtt caacctcatc gaggtcgtca ccttcggcta tttccacgac cggcgagacg
    40621 gccaggatgc cgaagtcttt gccgccatcc aagcccttcg tcggaccttg agcccccttc
    40681 atgtgccgtc ggccccgatg ccggtgtttg cggaacgact caagaaggaa tacgacacgt
    40741 tcgtcaaaca acagcctcaa caggtgagtg atgctggttc agccccggaa attctcgatc
    40801 gcgcggtcgc gatcctcaca gagttttcag gccaggactt tcaatcacgc cgcttcctga
    40861 acggcatgat cggctacgtc aaaaccttcc accccgagat tgcagagcat ctgaccaaac
    40921 agcacgaagc cggccggatc gtgctcccgg gccaactgac cccgcccccc caggagccga
    40981 cccatgttca cggcccagag tgccatcacc accaccacta aacccgcatc ccgtcgggcc
    41041 ggcatccgtc actgccccgt catgccctaa ccatccgctc ctgttaattc ctgcacctcc
    41101 ctacactcat tctctcttta gtttcgactg atctgtaccg atacaggcct gctaagccct
    41161 acgactgcgc gaaagagcgc gaccaggtga gtcgccacaa cttgctgaaa ctaatgcact
    41221 ctttcgttat ggatcttctg gtaagtctgc cgaaggaaga tgcactggcc gaaccggcct
    41281 ggagctcccg tcacggggga attctttcga tcctctggct ccacgtgctg agcgtgccga
    41341 tgttcggtat ctacatgggg gcaagtccca ccctctatct cgggggagga gggctacttg
    41401 ccttcatcgc cgtcgccgcg cggctttccg ttgtccgccg ccgcctccaa tctgccatcg
    41461 ccacctgcgg gctcatgaca gctctagccc tactgggaca cctctccgga ggtcaaattg
    41521 aactgcactt tcattttttt gtcatcatgt ccgtcatcgt gctgtaccgg gattggcttc
    41581 cgtttctcgt cgggctccaa tttattatcc tcgatcacgg agtggtgggt acgctcaggc
    41641 ccgacctggt gatcggacat gcccaagggc aaacacaccc ctcggcctgg gcctcgatcc
    41701 atggcggttt catcctgccg aatgtgcggc cctgctctac ttctggcgcg tcgatgaact
    41761 cgcacaagat gaggtccggc aaagcgaggc cagaactcgc atgatcatcg aaaccgccct
    41821 cgacgccgtg atcacgacag atgccacgcg gaccataacc gggtggaaca cccaggctga
    41881 gtcgatgttt gacgtcccgc ggaccgaggc gatcggaaaa tccctgacca cctacgtatc
    41941 cgcctcatac ccctcaactg aaccaggccc cgacactgcg tacgcggggt tagtccccgg
    42001 agccatcctc aaccggcgca gtgaaatgct gggccacacc tgccagggaa caagttttcc
    42061 cgtggaaatc gccatgagct gtctcgccat cggcgggacg cagcaattca ccatcttcgt
    42121 gcaggacatt tccgagcgaa aaaagcagga agagcgcctc cgtcgcgcga aggaggcggc
    42181 cgaggttgcc agcctggcca aatcccaatt cctggccacc atgagtcatg aaattcgaac
    42241 ccccatgaac ggcgtcctcg ggatgaccga actcctgctc acgactccgc tgaacgacaa
    42301 acaacgccgg tacgccgaca cggtgcacca ctcaggaacc aacctgttgc acatcatcaa
    42361 tgacatcctg gatttctcca aaatcgaagc cggccgcctg gtcttggaat acatcccctt
    42421 cggtctccgt cagatgctca ccgaaaccat cgggctctat cacgaacagg ctcgaaaaaa
    42481 gggactggcg ctgaccgtca ccattgcgcc agacacaccg ggcacggtcc acggcgatcc
    42541 gcaccggctc cgtcagattc tgaccaacct ggtcggcaat gccctcaaat tcgccgagac
    42601 ggggaatgtg gcggtctccg tacgtccgga cgaaggagat cagggccgag tccgcattga
    42661 agtgaccgat acgggcatcg gcattccgct gcacgcccag gagaagatct tcgactcctt
    42721 ctcgcaggcg gacggatcga cgacgagaaa atacggcggc accggacttg gactcgccat
    42781 cgtgaagcaa ctggtccgca tgacgggctg gcaactcggt ctctgcagta cgcccggaca
    42841 aggcacgacc ttttggttca cgatctccgt agacaggagc acctcacccg agacggaatc
    42901 gcagcaacac agctccgatg agaccaccct cacgatggcc acacgacagc aaccagcaac
    42961 tagaaggaga tccctcacat gcatgtcgag acttgctcca tcaaaaccgg cagtgccttc
    43021 tcccctgcgt cgctctccac actggattcg aaggaaaccc tcgtcctgct cttcggagct
    43081 ccgggcctga tcgatgcccc gcatcgtatc cgcgaggtcc tcgaatcctt tccccgcagt
    43141 cgcgtaatgg gctgctcgac ggctggggaa attcacggat gcgaaatctc ggacgacagc
    43201 ctggtcgtcg ccgccgtgcg ctttgagcaa acccccattc gcacggcgga ggcggcggtg
    43261 caatcccccc aggactcgta caacgccgga tgcgcgatcg ccacacaact caaacaaccc
    43321 tcgctccgag gcgtcttggt cctctcagac ggcctcaatg tcaacggcag cgaactcgtg
    43381 aagggattga acgacacgct gggagaaggc gtcgtggtca ccggtgggct ggccggcgat
    43441 ggcatccact tcaaacgaac atgggtgctg aaggatcgca cgccgcacag cggctatgtg
    43501 accgccatcg gcttctatgg cgaccacgtg aggctcggac atggttccaa gggtggatgg
    43561 gacaagttcg gcccggaacg gctggtgacc aaatccaccg gcaacattct ctacgagcta
    43621 gacggccgtc ccgcgctgca actctacaaa gagtatctgg gagatcgcgc caccggcttg
    43681 cccgccaccg gactcctgtt ccctctcgca atcagaacct cgcagtcgga tggaaaaatg
    43741 ctggtccgga ccattctggc cgtggacgaa gccacgcaat ccatgacgtt tgccggagac
    43801 attccggaag gcgtcttcgc acagctgatg cgcgcgaatt tcgatcggct gattcagggc
    43861 gcatccgaag ccgccacact cacggccaac aaccacatcg gttcgcacgc gacttcgccg
    43921 accctctcca tcgcgatcag ctgtgtcggc cgtcgcctgg tgctcggcga gcgaacggaa
    43981 gaagaagtcg aagcgacgct ggagattctg ccgaaagaca gcaaacaaat cggcttttac
    44041 tcgtacggcg aaatctctcc ctacgccagc ggagcctgcg atcttcacaa ccaaaccatg
    44101 accctcacaa ccattaccga gcactaacgt ccatgcatcc gctgctcaaa cgacaactca
    44161 aacgcttggg gctggacgac gcgacagcgc cccaatccct cgccagctgg caacagctcc
    44221 tcgagcgggt gagtcagagc tatcaggagt ccgatcaggg gcgcgaactc ctggagcgct
    44281 cgatcgcctt gtcttcccag gaaatgcagg agctgaatga acaactccgg cgcacctctg
    44341 agagcaagct cgcaggagaa cgggacaagc tgcagacggt ccttcgctcc atgggcgatg
    44401 gactctgtgt agttgacgga cagtggaaca tcgtgttgct gaatcccgaa ggtgaacgcc
    44461 tgtggggtct gaccgagaaa gaagtcgtcg gacgtcgact tcatgacatg atttccctct
    44521 cttatggaac caccctcaca gaaccgatct tcacccaaat tttgctggat gaaaccgcgc
    44581 agggagactc gtttcggacc gatgatggcc tactgaccgc cactaccgga cgctcgtttc
    44641 ctgtcgcctg tgtgctcgcg ccgatcgtgc acgatcatca cttcgccgga gccgtcctcg
    44701 tgtttcgcga catcaccgat cgcaagcagg cggaggacgt ccgtcgccac accgaaagtt
    44761 tgctgcggcg acaacaatcc gcgctgctgg agctgacgcg taacagcgta cttcagagcg
    44821 gcgttttgga gccggcgctc caagaaatta cccgcgcaac ggccaccacc ctgggggtgg
    44881 gccgcaccag catctggctc ttgagtgaag accacggctc aatccgatgc aaagatctct
    44941 acgaatcttc gagaaacagc cactctttgg gaatcgagct ccaatcggcc gactttcccg
    45001 aatacttccg tgaacttctt tcggagcgcg tgatcgatgc ctcggatgct agaagtgacc
    45061 ctcgcacccg tgagttcacc tccaactatc tcgagcctct cggaatcggc gccatgctgg
    45121 acattccgat tcgctttaat ggcaaactcg tcggggtgct ctgcaatgag catatcggcc
    45181 cacctcgccc ctggatgctg gaagaacagc agttcggtca tgcgatcgcg tctctggtat
    45241 cgctagccct cgaagcggtc gaacggctcc acgcagaagg agcgctgcgc aagagcgaag
    45301 gccggaccag actgatcatc gacaccgcac tcagcgcggt gatcagcatg gacgagaagg
    45361 gctccatcat cggctggaat acgcaggccg aacagatatt cggatggacg cgccaagagg
    45421 ctatcggccg cctcatggcg gagaccatca ttccgcccga gcaacgcgaa gcccaccaac
    45481 gcgggctccg gcagttcttc aagactggcg aaggcccgat cttgaacaag cgaatcgaaa
    45541 tcaccgcatt gcggcgcgac ggcacggagt tccctgtcga gctagccgtg acgccgctgc
    45601 gtctcgaaaa tgcctatacc tttaccgcct tcgtggtgga tatatcggaa cgaaaaaagg
    45661 ccgaagaggc cttgcgcacg agcgaagcac ggttgacgat gaccgtgcag ggctcgcaca
    45721 tcgggatctg ggactggaac ctcaccacag gcgcgatcta tttctccccg caatggaaga
    45781 accagctcgg ctacgacgac accacgctcc ccaataactt cgacgagtgg cggacgagga
    45841 tccatcctga cgaccaacca ttcgtgcagg agaccatcca gacctgtctg gatggccaac
    45901 gatcccagtt cgaacttgaa catcgtcttc gacatcgaga cggctcttat cgatggatcc
    45961 tgtcccgcgg cagtctgatt cgagatgttt atggcgtaac cgcgcgcatg gtcggcatcc
    46021 acatcgatac caccgaccgt aagcaggttg agcaggagct ccgtgcagcg aaggaagccg
    46081 cagaggccgc cagcaaagcg aagagcgagt ttctggctaa tatgagtcac gaaatccgca
    46141 ctcccatgaa cggagtattg ggcacaaccg aactgctgct caacagtgca ctgacggaca
    46201 aacagcgaca tctcgcgtcg accgtgcatc gttccggaag aacgctgctg gccatcatca
    46261 acgacattct ggacttttca aaaatcgaag ccgggaaact cgatttggaa tgcgttgact
    46321 tcgacctgca accggtgctc gaagaatcgc tcgagctgtt cggcgaagcg gcccgccgga
    46381 aacagcttcg cctttctcaa cacatcgacg acgcggtccc gcgctacctc aagggcgacc
    46441 cggtccggtt ccgccagatc ctcatgaatc tgctgagcaa cgcgatgaaa ttcaccgaaa
    46501 cagggggcgt ctcgttgagc accgaatacc tggaaggaac caccagccag gccctgctgc
    46561 gctttgccgt gaccgacacc ggcatcggca ttccgccggc ggccaagtca cacatcttcg
    46621 acgcattctc tcaggcggac gggtcgacca ctagacgata cggcgggacc ggtctcggcc
    46681 tttccattgc caagcagttg gtcggactca tgggcggtac catcactgtg gaaagcacac
    46741 cgggaagcgg ctccaccttc gcgtttacga cgcggttcga tctgcaaccc ctggggtcgg
    46801 gcctctcaac gaagctcgcg ccgtatatgc aatgggcaga gccccttgtc tcgtccggac
    46861 agacgagcga caccccatgc ccacacgcat ctgaagcggg agcagcagcg ccgagcccca
    46921 aacagagcgg gcgaattctg ttggccgaag acagtcccgt gaatcgcgaa gttgccgtcg
    46981 gcatgttgga gcaactgggc tatgaagtcg aggtggcgga aaacggccgg caggccctcg
    47041 cggccgccga gcgcgaccgt ttcgacatcg tgctcatgga ctgccaaatg cctgaaatgg
    47101 acgggctcac ggccaccgga gaaattcgcc gtcgcgaaac agcctccggt cggcgtcggc
    47161 tgcccatcat tgccttgacg gcgcatgcca tgcagggaga ccgtgaacaa tgcctcaccg
    47221 ccggcatgga cgattatctg agcaagccat tcacccagat gcagttgcag ggaatcgtcc
    47281 gcaaatggct gagtcaccat gagcctcccg cacaggccgc acagctgaac gctgctgctc
    47341 ctggaactca atccgtgcca ctccaaacca cgactgagcc ggcagcggac acctcatcga
    47401 cagcgcccgg catggacctc aaggccctgg agggcatccg tgcgttgcaa cgtcccaacc
    47461 ggccgaacgt gctggcctcc gtgcttcgca agtatctcga caattcgcga gaaagcgtgg
    47521 atgcactacg tgacgccatc cgcgccagcg acccgatggc cctggaagca atggcgcatc
    47581 gcctcaaatc aagcagcgcg caactcggcg ccattgccgt agccgcccgc tgcaaagaac
    47641 tcgaggtcat ggggaaccga aaacatctcg tcgatgccga cctaatcttt gtccagttgg
    47701 aatccgacta tcgtgccgct tgtaccgtct ttcgaaatga gatcgccaag gagaaccaga
    47761 catgaacccg ctgataccag gacgagcacc ctatgcactg attacggacg acgatatcat
    47821 catccgtatg ttcgctcggg aggcactgga gcaagccggg tgggtggtcg aggaggctca
    47881 aaacggaatt gaagcctgcg aaatttttca gaagtgtccg ccggacgtgg tgctgctgga
    47941 cgtcatgatg cccgggatgg acgggttcgc cacctgcgcg gcattacgcc ggctgccggg
    48001 cggcgaacat actcccattc ttatcatgac cggactggac gactttgagt cgattacgaa
    48061 ggcctacgat gccggcgcaa ccgattttat cgtcaaaccg ctgaatgcta tgctgctgac
    48121 ccacagaatt cgttatatgg tgcgcgccga ccaggtcctc caggaactcc gggagagtca
    48181 ggccaccctc acgcaagccc gcgatgccgc gattgagggc gcccgactca aatccgaatt
    48241 cctcgccacc atgagtcacg aaatccgtac ccccatgaac ggcgtcctgg gcatgacaga
    48301 ttggttgctg gacacgcccc ttaccgctga acagcttgac tgcgcccaga cgatccgctc
    48361 atcgggcgat gccttgatgg tcatcatcaa cgacattctg gatttctcca aaatcgaatc
    48421 cgggaaactg tcccttgaac tgctggactt cgacttgccc acatttctag accgtgtcct
    48481 ggcactcttt ggagaacgag cacaacggaa gggtttgttg ctaacttcgc ggatcgccga
    48541 ggacgtgcca agcggcctgt gtggtgaccc ggcgagactg caacagatcc tcagcaattt
    48601 gctcgcgaac gccatcaagt tcagtgagca cggatccgtc tccgtcctgg tggaactcga
    48661 tcagcgacca gccgaacaag gccttgagtt tcctgcgacc aggtatggcg aactgtcggc
    48721 gcagcaagag cgggtgacgc atatccgatt ttccgtctcg gacaatggaa tcggaattga
    48781 acccagcgcc ttttcaaaat tattccagcc gtttgtgcag gctgacgggt ccaccaccag
    48841 gaaatatggc ggaactgggt tgggactggc catctccaaa cagcttgttg aattgatggg
    48901 tgggcatatc ggagcggaaa gcgaaccagg gtccggttcc gtctttcgat tcactgtgcc
    48961 gcttcagcaa caattgccga acagcctctc cactagacag gcggcctgag caccaagcct
    49021 caaggctccc atagcaccct catttcccct cagcctatca gcgcaccaat catcacctca
    49081 tcaccacgca tgagtgtatc tattaagata ctcgatgcgt gaattcgatg atacggtcgc
    49141 gcgaagtatg cccgcctata attgctatca gattgaaatg cttagctaaa gtgcatggct
    49201 taaactttgc tttacttcgt cctgcgcaca tagtatacaa agcggtcgaa aagatcttct
    49261 tggtagcctt cttcagcatg catatggcag ctcttacttt tctcgcttag taggcaaaac
    49321 cctacaccca aggatcacac cagtgcattg cctgacattc gacatcgaag aacactttca
    49381 agtttcccgt ttcgattccc caattcgtcg acggcattgg ggatcatttg aaagtcgagt
    49441 cactccaaac acctgcaagg tgctggatct cctcgcccgc taccagacca gggcaacctt
    49501 cttcgtcttg ggatgggttg cggagcgtca tcccgggctc attaagcaga tcgccgaatg
    49561 cggccatgaa attgcctcac atgggtacgg ccatgagctg gtcaccgccc agacgcctga
    49621 attgtttcgt gcagacgtga gaaaagccaa acagatcctg gaagacctta cgggctctcc
    49681 ggtccagggc tatcgcgcgc cgggcttcac cattacgcgt gaaacgctgt gggccttgcc
    49741 gattctggcg gaggagggtt acacctacga ctccagcatc gtcccgatcc ggcacgacca
    49801 ttgcggcctg cccgggtccg atccctggca ccatctgaga cagacctctt ccggccccat
    49861 ttgggaaatc cccccatcca cggtgaacct cggcggagtg cgacttccca ttgccggtgg
    49921 cagctacttc cggctgctcc ccttcccggc gctctgcggg ctgttgcgac ggactgaaca
    49981 aaaaggtcgt cccctggtca tgtatttcca tccgtgggaa ctcgatcctc tacagccgca
    50041 catggaagga ccgctgctct cgcaattcct ccactatctc aacctcgaca agatggagtt
    50101 ccggatttct acattaatga atacgttccg attcggcccc atcgccgaac agatcgatct
    50161 gtcgccgatc atgcctctcg atccgacgat cttcccagct cttcctcctg ccgaaatcgc
    50221 gcaagacgtg gcgtagtcac gtcgatactt cccacgcagg cattgcccga cccaccggca
    50281 gcagtgcacc attgctcacg ctgcgccaaa gtagggattg cagtgtgtat gcggtttcag
    50341 tacggtaggg gtcgcctgaa cgaatccagc acaacgctct cacggagact gaccatgcat
    50401 cacgggagta ggaaccggcg acagcagtcg cccaacacaa cgggcctggc atgggcacta
    50461 gcctgcatta ttttattacc cgcagcggcc gccggacaga gcctggatga caccgtgcag
    50521 ttcatcagcg acatggccaa tactcacggc tttgtcagga gcctgagttg cagaaatccg
    50581 aaggcgggaa aaccgacgaa aattaccgag atctacagtg tcattccaac cgaaaccaaa
    50641 ctcggcacga tcgaactcaa tcacggacat gatgaggtca atacgggctt cacgcacttc
    50701 gatctgcacg atatcgacac gatcgagtat cagggccaag acacgaaaga gaacaacctg
    50761 gtcctgtatg gcgtccgcat aacgtgcaag agcagcggcc cctgcatcat gaagaccagt
    50821 ttctgtaccg gagccgtcac cgaactcgaa gcacagttcc cggaagatac cttgctgttt
    50881 cgctccgcct ctcatgccga gcgaatgacc aaggcgctcg ctcacttctt agggttggtc
    50941 cgcgcccaac agcagaacca acccttctga ccgatatcgg tctgctccga gccgacactc
    51001 atgaagagat cggcccggac acaccgctgt ctcacctact caataatcgt cacagtcatt
    51061 tcaacccgat cgatcgggtt gtcgcatggc cccttatccg caggactcgg tgcgccggtc
    51121 tggcacatga ctcgcggcag cgccacgatc ttgtccgcca ccccgatgcc tttgacgacc
    51181 tcgccgaaga tcgtatattt acgatctaag aaccgggact cctcgacgac gacaaaaaac
    51241 tgggatcctg cgctgtccgg atcctgcgcc cgcgccatgg ataccacgcc acgcttgtga
    51301 gggagatcgc taaactcggc tttgacattg tgccccgggc cgccctggcc atacgccccc
    51361 ttcttcagcg aatcctttgt gttgggatcg cctccctgga tcatgaatcc
//
LOCUS       DAORQN010000003        49301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_43_length_49301_cov_47.529546, whole genome shotgun sequence.
ACCESSION   DAORQN010000003 DAORQN010000000
VERSION     DAORQN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 49301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43_length_49301_cov_47.529546"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK614_00525"
     CDS             complement(<1..707)
                     /locus_tag="PK614_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80732.1"
                     /translation="MARARASEPQRSRSKQNAVEELNRGITTLRELSSQIDDLSRDGF
                     PYRDAVRARTELSLRESIRRLFGEKSPEYQAHKNHKLRIGNRTESAQSTTLLKELIVQ
                     LENQKAELLGLTPAETAPPPPAAPERLTLTAVPPTQPAVPVVPLQDPSPAPLATVSMT
                     NAPATGAAATTNVSQPTQPMAPIVVTTPVSSTSASPPVTQGTASQPIRDTSIQRLPPQ
                     PPCLGRRDSGTSAKRMV"
     gene            complement(815..1309)
                     /locus_tag="PK614_00530"
     CDS             complement(815..1309)
                     /locus_tag="PK614_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB80733.1"
                     /translation="MAAGHKQPSTILIVDDDPSALLVSTKPLRDAGYTVLQAPGSAEA
                     LRLYAEHPDPIHLVIADVFLPPPGFQLSVDRNPYPRVNGLDMVERLLQDKREIRVLLM
                     SGSPSSELQDRGLVRAGLPFLKKPFTSETLLSLVREVLAGPPAAPDQKNAPRPGNGDV
                     EWVD"
     gene            complement(1347..2150)
                     /locus_tag="PK614_00535"
     CDS             complement(1347..2150)
                     /locus_tag="PK614_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80734.1"
                     /translation="MASTTHAAENRPFWTEQALFHFGDDVFFTGRASCAPNIEDGRQR
                     AYVAAVQEVKNFTRTQEVGGFPLDTQMIFEDLHPADCAQGLITVWRLLRVPRTALESL
                     SRHSSPGKHRDISPIQAQITAIRNLTPRVGMLRDEIWNRYGLPRSIWARPEQGELIWD
                     YSQFGLIIVFNQDDVLTRWRLNGPHPRSSEDEPIPSRQDNPAGNDLPAIDLTVRLQQL
                     EEQQDLDRRRQAQRYCTIRFAEVPESLRPKHGSCEQREYERLKEQHPPF"
     gene            complement(2355..3044)
                     /locus_tag="PK614_00540"
     CDS             complement(2355..3044)
                     /locus_tag="PK614_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80735.1"
                     /translation="MKPANLFICLSLLFLATTGCHGYAFIETPTTDGFHSKLPSPYTR
                     AVVWGGRPDTVQSASTWLLKKGVLVVDQTKVLQAAADQKVSLTGYQYLETDVLRMAKL
                     VGARLVVFSNAEVGSWEALDWSSGFPHSQRVYSATVALRAVDVNTGEIEWSGKAQSTE
                     RFSNIEEGVSLLTCHALATAWGLRNPGAVAPESICPPGSGLMSTEMSPPKAHAAPASS
                     GRENLTPSMNY"
     gene            complement(3348..3479)
                     /locus_tag="PK614_00545"
     CDS             complement(3348..3479)
                     /locus_tag="PK614_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80736.1"
                     /translation="MIEFPLILIGYALLPLIAIQLMIMADRPQKTGEQNAFPQVEIL"
     gene            complement(3910..3986)
                     /locus_tag="PK614_00550"
     tRNA            complement(3910..3986)
                     /locus_tag="PK614_00550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3950..3952),aa:Arg,seq:cct)
     gene            complement(4007..5107)
                     /locus_tag="PK614_00555"
     CDS             complement(4007..5107)
                     /locus_tag="PK614_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB80737.1"
                     /translation="MGIFQRKDSEHWWMSFSHKGKRYRLSTGTANEKLAERIYAKVRT
                     EIETGAYFPELPEHHKTMAELLEKYYVEHSALNKKPRAHRRDRSLKTHLVKAFGHLLL
                     VDVTPAAIAAYKTARRQAGASGATVNRELGMARHAFNLAMKEWQWASRNPFTMVTREK
                     EPKGRDRWLRDEEERRLLAACPAWLREFVAFAVETGLRLGEILTLQWPHVDLNQGTLT
                     VEAATTKNGLPRTIPLSNRAMNVLRQRQGRSGNASLVVFANRKGGHRTLGTVNRAFRR
                     ALTRASLENCRFHDLRHTFATRLCQDGVDPYTVQRLVGHQDPKMTQRYAHHCTESLRR
                     GIEVLNRRGTGRLSRFCHGQAHSALETPEAVV"
     gene            complement(5236..5529)
                     /locus_tag="PK614_00560"
     CDS             complement(5236..5529)
                     /locus_tag="PK614_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB80738.1"
                     /translation="MSEESSASDPRALLTVQDVAAWLRVHPKTVYGWASRQAIPTLKI
                     QGALRFRREDLASFMEGCLVREQARSSRPNPRRSGHHPDVPAVLARVKRLIQA"
     gene            complement(5577..6071)
                     /locus_tag="PK614_00565"
     CDS             complement(5577..6071)
                     /locus_tag="PK614_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80739.1"
                     /translation="MTTYPPFDSLRVRLREARAHGRRVTSPTLARQRFQVIVDTVIGP
                     MLRHVAHVLWQEGVPASVLTELDADPSHIAIRLDEQALAIYFWAATDPDHLRWAIHGC
                     NGYGPTHSVPYDFLTASHLASLLEQTLTALLGLPTPTGECTAQASHGVPSGAGDTSHH
                     EPRL"
     gene            complement(6178..6549)
                     /locus_tag="PK614_00570"
     CDS             complement(6178..6549)
                     /locus_tag="PK614_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80740.1"
                     /translation="MNPHLHRDPSQSPSLPDLNAVSVTGLCLHPPDLLAGMTGELVAI
                     ALIAIEGKRQPSTWGTLERQTYVVHAWALGSLAERMATLEAGTRLRILGSMDYLYRAG
                     AKSPTEHAILAIRIEELAVLP"
     gene            complement(6621..7616)
                     /locus_tag="PK614_00575"
     CDS             complement(6621..7616)
                     /locus_tag="PK614_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80741.1"
                     /translation="MRQVEVIRSQQEEQHTYYLNPLTLPVGLFACPSSIWGWGLNVLA
                     TIVSPDRQLERHQEWLDITWTGCLMALMIARSNRQLTAVDRIVEHKLEPDSRPLAEGR
                     VTVSWRGEREILVTYPVNEDGRVLIRLSHLATALRQDHIAFAAVAHGRIELAAWHRDR
                     LLHRWPLEIAAGQLEAAVRLEVPVMAPRSRWPHSLVFKLTMDPLPPKLPDPRSTIERL
                     LLQQNLPVVAAEEQQVLVRREIEQNLQAIVEDNHAAGPGHWRAATVLLVLAGQRDATR
                     SNITLSCFNVRTRELLARIDVTAGPDGLPGALDVAAIRLQDLLRFLSRSATDVAR"
     gene            complement(8097..10745)
                     /locus_tag="PK614_00580"
     CDS             complement(8097..10745)
                     /locus_tag="PK614_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80742.1"
                     /translation="MIGLSPFRFMSMVAGTLDTLRPILLGHHHFLNDLVMSVSFAILS
                     HLKSTVESSASVSLPVELSPKSISTLQEKCKDIKRKSTLPEYALAYLLHHLQDLQLIH
                     GPIPRIRLDTKRDLAASFHPDEVRLWSRTDEWWQKIRKTVFDTELPKPSIDGADRLRL
                     ALHDEACIALLGLIMEGDLLFSHQIAPLTRICVTDLCIEEGFLQIPWVPRRYKKGSGQ
                     NQENTPPGFLTESFWLPPLAQLLLLRYIRMRLLWGRHVDEPLVLDDPRSHIFHEYIRF
                     HPDFFIEWLKRYPIKFRPHQISADLALRVEESTRSGSLLGKIIDARRAHRVTTVGPIF
                     TALRSRLLVTRPPTTESWYKVVLGKRPPNLNPPRTTKQPKPALGPTPQLLLPAPSVAY
                     ASPASSTLPLTEEQHRQQDATVDACRAQLQPLQHRVNALLRVISKTTPMGERNILAKR
                     IEEFAQDLPLLPPTEAYSQWAPLSNFRLQLEWLRDLLTTRQDPLSSISVRNYYCTIQA
                     RLVQTLGARNVLNLATEDDVAKVVSGMLDTDLAGETLKTQRAHFVRLFAFLHFNHGVA
                     VVNLSNKSLWVGSEIHPFPLISPHEFDALIDDVSPPDKIAIILGGYGMARRGELARLL
                     VNDLSGTAKDLHLSIRRSKTRAGIRRVPLWALIPDRHIQTVLAYRASREEEGSFNGMA
                     PLLVDEQGVPFTESELGDRISQTMSRAGLDVQSLHPLRHQGASWFPLVWYAAFHQLPS
                     NTLGFNFAHSLFEKRYLAQFRQLFVSLPHLDDDIHHPWDTNPFHVLSRIMGHSNPLTS
                     IQRYVHTLSFLEYLLMQREEYIPDSLKNIARTQAARLLVCSPSEIARGFKTATGPGGP
                     HIDKQIPLLDVLDKLRASVRKRSSNR"
     gene            complement(10757..12766)
                     /locus_tag="PK614_00585"
     CDS             complement(10757..12766)
                     /locus_tag="PK614_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80743.1"
                     /translation="MAIAPAHQFVPAHGSPYIAAAVANFQSRGRGKTHGTSPVEATVD
                     HFARTVRLAFGNQLLTPVDARLSTRVADDEVMCGDLPEPSNKKFNDDDEESTDGTKTF
                     HHSHESTRTSALQEGESAEEHSKLSATAPEADLPVTDLLTWIHHRALARDARIHSSIA
                     YWWERSCPTLSIDAQLWHLVQCGQPEAAVAWLVKHTGLNPESALGLKLLRTNKGIPPE
                     GMFLDRTTRTLRYVRPLEWCGFNSDHLLISLDGCEETTRDITIPLPPLIWKGIEPYLH
                     HRLTHDSALAGTEFADFFFLRSNNPPRGLSVADVNRFLRDEITTSESVTLRSLSRAFK
                     LLYEGRSALNEILSCYISGRVPYFLRTAVFYTRVRLADLAQHYWDASTQYARALLQEL
                     TASRLPADDATTVLRTLVEGAPRPPDEIVSASAAFGSKAVPRIDTVTSFFQGYRNCLA
                     DLAAATSLQDRRQYFNHFMIYSFLAFGYASAIRPVSDANLRDHHVRHDSVDTDLVLVA
                     DKANQRYDDSRFIPLSPVAKSLITQVLRARQMFIVYLAMRRIHRYDDLAQEEWPIFFL
                     FLPDFRIRLLTPKTIRQHLEDIGLGNRYRVPLNGNRHFFRSHLWQRLPSRLLHAILGH
                     QHQGREWMGRVSVSALDSAYDVLTGEIDTMLEILDISPISFTWRR"
     gene            complement(13321..14154)
                     /locus_tag="PK614_00590"
     CDS             complement(13321..14154)
                     /locus_tag="PK614_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80744.1"
                     /translation="MAKDFMQLSSHKTTREREGQQIYPPTGDGLKTLLIERAVSEELR
                     VYFEQIIGFIWDPSAEHPIACPISIKPDKEPIRVSQRRLTLSEANLFVHPMAEFLASI
                     IATTPTLKPRGLSKEITPNVAPSIGLGTRDLVLPSEYFDEELLQEDLKGRPLRCLELG
                     PNKYLYVDSVRRLAELQAHGYRNVPFLVAKSSTNGPWDQAILRSFRRAFESLREDPSF
                     LRPEVRRRLVNVLVSVGLAPLIFKSLTLSKIYEVMQVSASTTRAGRGKPVTADEPCTT
                     T"
     gene            14375..15373
                     /locus_tag="PK614_00595"
     CDS             14375..15373
                     /locus_tag="PK614_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80745.1"
                     /translation="MMKKKELILKELDHLIETAYFKTKPLQRKFLQHFSAVYRTSDEI
                     VTVRNQDLVAIYENETPEIKNDGNKFLSKKNAVSKHIGKLRENLKEFYQGPGKARQHV
                     ISIPKNEHGNYSLDVLSRQSKKGIREGPPKAVQGNVLVLSDANNTGSVLRPQSVTEII
                     LLVLKGVDRKRLVQIQRAAAGIMWGVLLVGLAVLIVLLIGIPYWPAMPLMGLSLAGLL
                     MICTCIAVLARPITRLSDMPFRRWWGDTFCMYRREALVLARYSGRCMVDGCYGHLSVV
                     QEGHERTDLFYPLPRRWIIVCKNVPTEHPRMPFDFTALDRAQAPNGSSERELARRA"
     gene            complement(15830..16138)
                     /locus_tag="PK614_00600"
     CDS             complement(15830..16138)
                     /locus_tag="PK614_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB80746.1"
                     /translation="MTLNNRRTEGKAVDTLLRDWDRMVTFYSFPQEHWRHLRTTNIVE
                     SPFASVRLRTNASRRYKRVEGAKAIIWKMLRIAETSWRKLNAPELLPLVTSGLTFKDG
                     "
     gene            complement(17174..17896)
                     /locus_tag="PK614_00605"
     CDS             complement(17174..17896)
                     /locus_tag="PK614_00605"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF019889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRB80747.1"
                     /translation="MTWRSNQIPACALPTSQVAQAKFLTLSTLILLVVPDTFATGTLF
                     ECVDAHGKTVFTDSPRQLLACHALDFSSLKTSTAPSAPASAPSTSGSGQDTGDSLPTA
                     PVLGGPLTTAVPVERLGNILVVSATLNGSKQARLIVDTGASQTIISQRLALDLGLYST
                     AHTTHVLLHTASGSVRADAVILDSIRIGGAEIRNSQVLIHDLPDLPFTVDGLLGLSFL
                     GAYQITLDTSRGQLHLKSSPEK"
     gene            complement(17880..18551)
                     /locus_tag="PK614_00610"
     CDS             complement(17880..18551)
                     /locus_tag="PK614_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB80748.1"
                     /translation="MMVAQEPSSSNADIKELNPTSQAGLLLLSSSFQLLYLNPEARVL
                     LQEFTLRPMPSTDLPPAIRSFCQEVMQTLPASPEPAAWEGLRLSRVTFSTSRPILLRA
                     FGVPAPLNEGDGQLLLLIEPAQPTYQALAPHPPPPTPIHLTPREKSVTRYLLEGFTNK
                     EIANKLRISEHTVKDHLKRLMKKTRVTTRTAVVSRLLGDHNLVAWLSNTTTPAVGQDI
                     HDLAV"
     gene            complement(20321..20857)
                     /locus_tag="PK614_00615"
     CDS             complement(20321..20857)
                     /locus_tag="PK614_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80749.1"
                     /translation="MYRTTGGITPQIVFAIAIKITAAGGNSVAAPTAPPDAAIDKGIR
                     AVGQSNRCIASTMAPNVGEPIAIKICEPEGQTIPTPITQPHIAASERIGSIRKSDRGI
                     APAMAPHVRFAVAIDISNPKGRAADTPIRYPITRCLECCACRKSYHYLRKGPTSNLTP
                     EVLLAIAIKISYTQCRSI"
     gene            20864..26215
                     /locus_tag="PK614_00620"
     CDS             20864..26215
                     /locus_tag="PK614_00620"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB80750.1"
                     /translation="MSSSVVVPDVPSWLGIWCATGIFGVGDFTGDGKHDLFCQESGQV
                     SIARSGSYPGAVDLLVIVSNGLTGTSTLSYTPSTQYSNTQLPYPFQTVSSITTCDNWV
                     SATETCAGLSATTNYTYAGGYHHPAERDFRGFNYAKVTGPVGPAGEQAITETWFHQGN
                     DVAVGANNPAAPNGYTKGLPYRSKVTDATGKVYSETTTTYVADADGVAPFFTPVAQVD
                     TSIDNGAKQTRVVFPITSGYAPAYDDYGNVMMEYHHGDLATTSDDRSIYRVFGVNTTD
                     WIVGLQTRESVYTGIGTASVNMVAQTDLYYDGTTSCATASVNQVPTKGHVTRTVRWLN
                     GGTSPETRMAYDAFGNLTCTRDALGQTSTVTYDASNTFAKTATNPLGHVATTVYYGVD
                     GVLMDKGLYGQVKSVTDPNNQTTTSEYDALGRKSKTTTPDGLITTATYNYGTGFTVGT
                     QHVLTSTSGAGLATILTTATYFDGLGRTIKKESTGPDGKTVVTEVQYDSRGAVRQKSS
                     PYFKTLESVTGRWAITNYDALGRVVRIDRPDSTRGLACFTDWVTVTIDAADHRKRETK
                     DAYGRTVRVDEYQSTVSTCDTTVGTPYATTTYQYDVQGNLLAVTDAKGNISTMTYDTL
                     GRKTAMHDPDMGNWSYLYDAAGNLTKQTDAKGQVLWFQYDALNRRVQKDFATQKTFGA
                     GDVRYTYDGTTNNRKGRLQQVVDASGTVAFQYDSVGRILQTEKSLDGTTYTTQSTYDG
                     LGRLLTVTYPSIPSKTISYAYNGPVLDKVFEGTTTYIRYTNYNALGQAGTTTYGNGVS
                     TTKTYANTANAVCTQQNFRLCTLKTSGPGSGGGSGSTGTLSGSFTATPSTVNLSTEGT
                     TDWAHWGLVDAASFDHKSGVSSQLNTYSTLGGGTALRYDDNGDGYTWTGGTPTASATN
                     STTGVYVVGLNKGFQIVVPADTTSRTVKLYLGGWAAQAKLEATLSDSSAAAYTETSFN
                     DAGVNGTNRVYTLTYQAASASQTLTVKWTVTGMNDPQWGNVSAQAVTLVINSGSGGGG
                     GGSSTTYNAQADFSITQGQQGWYYLSSTGAQLTWNGNFWAGTDGYIGLWNDGGYPGNS
                     TDSVRRWVAPAAGSVQITGQAILAETCGGDGTTVTVKKNGTVLWTEALPVHTVVHSFS
                     LSNTVVANDQLDFVTNRTGTSFCDNTIFTPTIVLTTGSGGGSSGTAYQDLRYVYTPDG
                     NVSDIYDNLVAAGAGDQHLSYDSLSRLTLANGPYGTSGTNASLTYTYDELGNLTFNTQ
                     VGTYTYPTSGASSVRPHAVTMAGSNTYSYDANGNLSSGAGRTLTYNLENKPLTITSAG
                     QTTTFVYDGDGGRVKKIAGTTTTRYISKLYECDNASCSRMIFAGSARIATIGSGGIVY
                     YHPDHLGSSSVITDSVGAKLQALTYFPYGATRTNTPSGTPTIDVPYKYTGKELDSSTS
                     LYYYEARYYDPTLGRFLSSDTIVPSPLNPQDFNRYTYAGNNPFRYTDPTGHFKIGKFL
                     DRVFDKPWARAIGWAINPMIMQFLDPATRRTTVPVAAGVVGNFVCGPICGGMASGATR
                     AALVQSEGRRANILRSTLAGGVGGLAGGVANLAVPGDMVMAGLAGGMANGAVNAAIGG
                     GSVLQGALMGGATGALTGYYNSQGIIDRTTGPIWSGPNTLIGTGYLLLNTIAAGPVML
                     MGGEGMGYAYGNGGFQFWNAPLMMGGIRLGAITLMGGVDGNGIPFTPDYNGPSPYEGH
                     QQINLGRHEGGHGIQQEVLGPLFIPLYFLTGGPHEANPFEIGADNYSTGNGSPWSGF"
     gene            26422..26682
                     /locus_tag="PK614_00625"
     CDS             26422..26682
                     /locus_tag="PK614_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80751.1"
                     /translation="MGAPRSDSNTLRNEVTVEAVTGEIVARYSEQVNEYARSGRSFTP
                     HWLLSDKGLFFIPRKFENNWQGNIPVIEKTGQFFIPNQLKVQ"
     gene            complement(26790..27107)
                     /locus_tag="PK614_00630"
                     /pseudo
     CDS             complement(26790..27107)
                     /locus_tag="PK614_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27384..28504)
                     /locus_tag="PK614_00635"
     CDS             complement(join(27384..28249,28249..28504))
                     /locus_tag="PK614_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611496.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB80752.1"
                     /translation="MKRSKFSEEQIVYAIRQAESGTPVEDVCRQLGIAEQTFYAWKKK
                     YAHLGVSELRRLRQVEEENARLKRLVADLSLDKHMLSEALRKKSLRPARRRELAHWFQ
                     DTFSVSCARACRLAQFGRASWYRLSRAKDQSALRLRIRDLAHARPRFGYMRIWVLLRR
                     EGWPVNRKRVHRLYRLDGLQLRMRVRRRKHTALHRGPAPIPVGPTERWSMDFVHDTLA
                     DGRPFRILTVIDNWSRCSPVLEAGFRMTGETVSQVLDRVLRQGRSPRSITVDHGTEFQ
                     SRALEDWAYRRGVQLDFIRPGKPVENAFIESFNGRLRDECLNVHQFASLAEAQAIIEA
                     WRMDYNHHRPHSSLGHLTPNEFVAQRQGQPTIEEVVCSG"
     gene            28882..31323
                     /locus_tag="PK614_00640"
     CDS             28882..31323
                     /locus_tag="PK614_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRB80753.1"
                     /translation="MHEPALRPWSGITALVLVATLLTSCASPTLRRAERLAEDGEWDK
                     AVAAYRETLKNDPFDEALKDKLEVAKQHAAVVHYANGRRSLEERRIAEALSEFKAALG
                     LDPTKVEHHAGMTDALRLKEANDQLQTADKLLNLGRSEEALTAYERAVELDPSLTQAL
                     THITEITAQQRATKMFGGSSQPISLRFQNAKLKEVFEIVARTAGLNVIFDKEVRDEPI
                     TIFIKDMAFDDALNLILNTNGMVAQRVAPETLLIMPNNKQKQAQYQDLLMRTYYLNNA
                     KAKDAVNLLRTMLESKRIYVDEKVNAIVVRDEPVKLQLAERLLFAIDRREPEVELDVE
                     VLEVNRTKSLKYGLNYAKQAGSGLVPTGGSGGISTATTQFTYQQLTSIGPQSYLFTLP
                     ASVILDFFKQESDAQTLASPKLRVMNNKSASINVGDKQPILLSTTNVLPGQAVTGAVP
                     TTSTVTSIEFKDVGVKLTVEPTINLMNEVMMKIKVEVTRLGDQVTLQAAPEIKQFKFG
                     TRTAETVLMLKDDETVILAGLIQDEKRKTKSTIPLIGDIPILGDILTSKQDDRVSTEV
                     VLTITPRVVRNMELPSSAAQAFWSGTENTFATSQLYAPQAIPVSQSSGQSQRSHTPPA
                     PLGSVQGTPSVSPLHTTPTHDTMLAPGTKSPQGIAPTSDVSSLPSGQGQALATPVPAA
                     ELVARSTGSFILKPLDVATAVGQEFRVDLTALHLEQVSETSVMVTFDPKMLEFRRVTP
                     GSVALASRTADGQVMLTLRRDGTVPPGEGLLAMLFFHAKSPGQFPVDLQVLPGKASTM
                     GTDVTGTSRAIIHAR"
     gene            31332..31718
                     /locus_tag="PK614_00645"
     CDS             31332..31718
                     /locus_tag="PK614_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB80754.1"
                     /translation="MKPTVKLTNRGFTLVELMMVVTIVGILATLAIPSFQRSVIKARE
                     GTLKYDLATLRDVLDQYRADKGTYPSALGELVQVGYLKRIPVDPFTHSDQTWQVMADE
                     GESGGIADIHSGSPLVGPEGTPYNTW"
     gene            31846..32526
                     /locus_tag="PK614_00650"
     CDS             31846..32526
                     /locus_tag="PK614_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRB80755.1"
                     /translation="MAVAREREAELSTLRVEMAATRIAAAKKEAELVELRALVAQLRQ
                     ENADSHQRLLELRQAGEVRQAEVNALKAERDQLLVTKNDQSLKDLKDGLMVLTKQLED
                     VRQDIGHKTLRASDKTSPAPMGKTSAPSRPAMTPSSLQGTPAQFYDGPASISAISVLA
                     VNATQSPSDVLRVRVQPGDSLSGLARRYRTTVTAIAQLNHLPTDVIAVGQGLLVPSPS
                     SSPSDRER"
     gene            32530..34251
                     /locus_tag="PK614_00655"
     CDS             32530..34251
                     /locus_tag="PK614_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRB80756.1"
                     /translation="MPTLVRQATLLDVLVRRGCLTAAQAEEASAQVSGPERQLYVSLV
                     GGGLISDVQLAEALAEQCGVSYHSLHEFRVDFEAFSCIPIDIMRQFECVPVGEEDGEL
                     LLALADPTDVRRLDHIERAVGRSCRILVAPRSAIAESLRESTRNSQVVAKIKDEFRPL
                     LVHENDEGEEVLSVDKVQRDLSPIVQLVDTIILNALQKRASDIHLEPADGVTEIKYRI
                     DGVLYLAMEPLDLRFHNPLVSRIKVMSDLDIAERRVPQDGRFKLRLDKRTIDFRISVI
                     PSAFGESVVIRILAKEEFAAKRNGLHLDSLGLKKEDLDRFRRAITASYGMVLVTGPTG
                     SGKTTTLYAALNEVNTKEDKIITIEDPVEYQLAGVVQIPVNEKKGLTFAKGLRSILRH
                     DPDKIMVGEIRDAETAQIAIQSALTGHLVFSTVHANNAFDVIGRFVNMGIEPYNFVSS
                     LNCIMAQRLVRTICESCKAPLAVSQTQCEQLGFDYALFKDRPVYEGKGCLDCHGLGYR
                     GRHAITEFLPLTDRIRELMLDRRPPSEIRRAAVEQGMTDLRQAGVEKVRRGETTMREV
                     NRMTFLG"
     gene            34256..35209
                     /locus_tag="PK614_00660"
     CDS             34256..35209
                     /locus_tag="PK614_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80757.1"
                     /translation="MRATLFPIHRPRVGISVSSSSLSVVVLRRPWFRMPIVEQVLERP
                     LPGGLLKVSTTEPHMTDVDAFAKELRELTEGLSERTIALSLPNRAMHMGVFAFDRFPH
                     DPEEREGLIKWRFREDLNLTIGDGRVMMQIFPAGRGTQVLAMAVRQTILDQYEAVCSR
                     AGLIPASIGFSYIQLIGLYRRLMKGHRRVCVMQWADDEFVLLLFDQGKPVFLRTRSMT
                     GRTHDLRRELVGTLQYMADQATTAHEAGESSFPLYLISSQSKESITQLLGGEATVKVP
                     GPQGGWQIEIVPLGWDSVPAHLNNQTGSLNGLSAMATVVGL"
     gene            35206..35877
                     /locus_tag="PK614_00665"
     CDS             35206..35877
                     /locus_tag="PK614_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRB80758.1"
                     /translation="MRDLVGLAWSPFLRSQSATNPVPHFALSVTPAAITILRVMQWMA
                     VVVFCASLAVAFKWWNDSGVMEETATHLDGVVQRLNHANASRSEGMTRERLTLSVDQI
                     ATIKRDVRFVNQLAEKRTFSWSRLLADLETTIPPHMALRTVQLNFQDSTVALHGSAAS
                     LQDIHTFMTNLEHHAAFRHAALASHRLEHQESQPSQASMATPDTMQPARGVDFQLTVG
                     YRPSL"
     gene            35959..36495
                     /gene="pilO"
                     /locus_tag="PK614_00670"
     CDS             35959..36495
                     /gene="pilO"
                     /locus_tag="PK614_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRB80759.1"
                     /translation="MSVTVFACILAAAIYCYRLSPTQEHLAQAEAAYQTALRAQAALQ
                     ANRKTQEEMRAIKLQLDEVWRGLPTETEFASLALAISELGRSERVNIPGMQYGVEQSK
                     ASGMPVKASISFSVTGDYAAVYRFIHRLESADSYVVIESLDASRAAKSDKGVSSSVLF
                     HVKVVTFLRPNPPSGSMS"
     gene            36492..37148
                     /locus_tag="PK614_00675"
     CDS             36492..37148
                     /locus_tag="PK614_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80760.1"
                     /translation="MKIRTKTLILGGLVGLWAMVAVVQIGSLPESQEAPLTYVSGQRV
                     ARGVKSTGTDGEVMLRPMRVGVAQLPATPKRNIFEPAETPPSEKRLVRAEASKKEVTP
                     PMPVTVVAMPVVPPAPPMPSPEELAEQAARQAQERKRHQLRELMAQYRYLGYLTREGE
                     QKAFLGKGQEIYIIHAGDTLDGQFQVASITPTAVKILAAQSDVETTIQLKTESSIEPS
                     "
     gene            37411..47031
                     /locus_tag="PK614_00680"
     CDS             37411..47031
                     /locus_tag="PK614_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB80761.1"
                     /translation="MTHATVCTGMLRRWMKVVVLAGLAGILGPFSSALAQSSTVTLDA
                     YLHGSGGTANPPTLTSDLIPPLSLTAKTKDSAGLAFSGGNPWVTMGSWTPPAGINGKV
                     TLTALTTARLWMGLKSSGDVGGKVDLLVEVLVNGQVLSSGLTRCLSTLTASASTAQEI
                     SIALGSIPTTQLDTLTQTLAVRVSTRMGTTASNTACGTKASVTGVRAYFDALTRTSRI
                     GVTIALPPPQPVILLPNPKTIQAGSTSTLLALLFPVPLQATTLSVVSTATSVATVPSS
                     VAIGIGQAFVSIPVTGVSAGTTQIKVTLNNKTITGTVRVVGGAATISSLTPSTLSITQ
                     GGSGQLTVSLNATQATNTVVALSSSADSIAAVPATVTVPAGQVSAAFNVAANTAGQAD
                     ITGTLNGTSATSHVTVTPALPEVVSLTPPASQLTLGATTSLTVTISAAQVGPTVVTLS
                     STPTGLVTVPPSVIVPAGQTTASFTVTSVALGTTMVRATLGSSLAEAAIDVVPPVVAL
                     VDLQPASQSLVVGAIGTLTVTLNAAQSSPTDIALSVDQPTVLQIPATQSVTVPPNQTQ
                     ATFTVTALATGSAQLTATLGTISKQATVTVTPQPPQVLSLTPSPLAVVQGATGTLTVT
                     LNAAQATDTVVPITSNNATIVGVPVSVTVPAGLTTAPVSVSGLTIGAATVTATLNGTA
                     TATVNIVPPPPVVTALGPIPPVTAPLTLAKGRTGVLQVTINRAPTDPTVITLQNSATS
                     VVTVPPSVTVAAGQLTASFPVTTQTEGQATITASFNSTSATAQVVVTAPEVESIALAP
                     AAPTVFAGESVSFTATGLFTDGSSQPLANDVTWSSTNQSVASVSSSGLVTALTVGTTT
                     IKATVTTSVGPVTGETTLTVQPAPALVLTPLTTSLTVGQSALFTVGTAVAPTSALTVT
                     LALSGSGTALLNPVSVVISAGQTTSPTPVQVTATGIGSVILTATAPVRQPASSTLTIT
                     AGLPVITSVSPATGVVGTVVTLGGSSFNTVAASNQVRFGGIVATVLAVNTTGTSLTTA
                     VPPGALTGPVTVTTPLGTATSPLPFTLLNSPPVISSIGNKTLTLGSTLAFTVVGVDPN
                     GQPVTYGAQQAAPASPTLPSHMAFNSQTGVFSFTPDATQVGTFNITFLASDGVVSSSE
                     TVAITVTGTVPGAQTSLSGRVDDSSGNPVAGVTVSLKDSPTTTTTNASGQFTLTFAGT
                     PAGRQLLVVTGVPSGYANLVAPVDLIPNTANVLPSPVTTPPLDLATAVTVNPAATTTL
                     TSATAHVTVTIPPGRAKNADGTAFTGQLTISPVPEYGRPESRPVELRPGLSVTIQPAG
                     VILDPPVPITFPNIDNMPPGNELDLWSLYPDTGTFLVVGRMRVSADGQRLETIDGGVR
                     KTAWHYALPPNPVPNQVPTQQAGPCTQCAFASQGDLTEGALTETVAIPGIRSLGVSRN
                     LTLRYTSTTADVQPILPVNALLSVRAAVPPTFSARLSVGGVQQGQEVYWDSHALPEND
                     NSTSRLGLQMDASNLSTGSYPYELMLFSNYPQSSIGGASIARMLVRNEQQNPLGAGWT
                     LDGLDRLYPQPDGSAVLAQGSGSTQWYRPVERPLVIESFDQARSLTVGGVNASFVSGA
                     IHTGARNDLLNPANFGAQGKVRRPVTLRSGINTLTLSALTGVDLFVLNLLATEITATE
                     AQVLEQFVREGGTVLEIRNAAPARPLLWGTVSGPFVGDSTADLTLDGLNAMVSNGPFG
                     TVVSPLLTGGNAGYATTGSATVLARNDVGPNVLLLPPSTVFSGAGRAVLVGDEEIVAS
                     GFPSGAGSNRYAEGSNRTFFLNTIAYLSDAPGYNPVLPPNTTVVTLQGPPGDFSVLAR
                     RTDSTYTRILKDGTTYQFTAQGLQTSVSDRNGNQTTYAYDGTGQLLTITDPVGQVTTF
                     AYSGSRLATISDPQGRVTQFTHDGGGNLTGVTYADTSTATFAYDPQHRLVKKTDAQNQ
                     VTQYAYDYAGRLSQATLPGGDRRSLTPSQRMAVPNLAAGQGTATTPAALSPSTINQAG
                     FTDAKNQTTRFETDALGRITKQTDALNRVTTIARDVNGNPTQIARPNGAITTMTYDAK
                     GNLLTSTEQATAGTTTFTYEPTFNQVTSIRDPKGNLTQIAYDAKGNPLTITDALNQVT
                     TFTYNPQGLLLTTKDALNQTTTFTYDALGRLLTTTDPLTRTTTLTYDQAGNVATSKDA
                     LNRTTSFAYDPKNRLTQVTDPTGGVTRYAYDGNGNLLTVTDAKNQVTTFAYDSRNRLL
                     STTDPLGKIETYTYDGNDNLIKRRTPKGDDILFAYDAVNQLLSKTLPSSQVTSYLYDL
                     VGNLTNVTDPDSVLAMSYDLANRLTTVKTDGSPNQPAVTLSYTYDKNGNRGSVTDPAQ
                     SVAYRYDGLNRLSQLGVPGPASCPTLPSGLVSRWLADGTTADAVGSNPATLRNGTTFG
                     LGLFNQAFHFDGVDDFVEVPHAASLIPPALSLSFWLKLDTLTSTSVSEQGLQWVFFKK
                     NSRPSTTLFNDYGFAKVFREGRHYVVFGINAASGQPVFVNSTTSVVAGVWYHVMATVD
                     AQELKLYIDGALEGTSPVGFPLDHSTSPLIFGATNQSIYDGKLAGSLDDIQFFNRVLS
                     PAEVPQTMCQDSAPLGQATTYTYDALSRRTSLTLPNGVQTTYQYDPASQVTAISHQLT
                     AISQQINKAEYGYNAVGNRTSLTDRRGVQAFGYDQLDRLTSASHPLLLDPQAFAYDAV
                     GNRTTGGSVVNSGNQLTSDANFAYQYDDNGNLTRKTLLATGNYTQYTYDAENRLTQVQ
                     EFAAGNPTAATTSTYRYDGLGRRIEKVANGQTKRYVYDGANVLQARYTHGHGIDEPLS
                     RTPVTPGVGSGHTAVLTSTPDGLRGPVVDDGISVNGQVSVGFTLVSGQPTPAVGQPID
                     STGSHQPIAPIDVTAATNSGTLTVNLIDTGGIGGNSALYLVTRDQATGQAIESRLLFP
                     ARVTFASTTPLGTPLVVASTTVPTTTPVADGTLFYHQDGLGTVTELTDSNGSVAKAYA
                     YDAYGNLLESPGTVEQPYTYTGREFDAETGLYYYRARYYDSMTGRFLQKDPIGLGGGD
                     VNFYNYVRNNPPRLADPFGLYDIATPGEVLNFWGEFFGAISDFGANYVDMRQAWWKGA
                     DKYFHCKANCQAAQRGVEGEAVACIISDIREWWDQNVKSYWEPRSTPQDSAADQQANL
                     YGRIQGSTNPGALCEQSCAPFRPAGLPPQY"
     gene            47588..48502
                     /locus_tag="PK614_00685"
     CDS             47588..48502
                     /locus_tag="PK614_00685"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HRB80762.1"
                     /translation="MSQLINKANYVYNAVRNRTSLTDRRGPQAFGYDQLDRLTSTSHP
                     LLLDPQAFVYDPVGNRTTGGSVVNAGNQLTADANFAYQYDDNGNLTRKTLLATGNYTQ
                     YTYDAENRLTQVQEFAAGNPTAATTSTYRYDGLGRRIEKVANGQTKRYVYDGANVLQA
                     RYTHGHGIDEPLSRTPVTPGVGSGHTAVLTSTPDGLRGPVVDDGISVNGQVSVGFTLV
                     SGQPTPAVGQPIDSTGSHQPIAPIDVTAATNSGTLTVNLIDTGGIGGNSALYLVTRDQ
                     ATGQAIESRLLFPARVTFASTTPLACPP"
     gene            48499..49272
                     /locus_tag="PK614_00690"
     CDS             48499..49272
                     /locus_tag="PK614_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB80763.1"
                     /translation="MIASTAVPISTPVATSTLFYHQDGLGTVTELTDSNGAVTKAYAY
                     DAYGTILESPGTVDQPYTYTGREFDSESGLYYYRARYYDATTGRFSQRDPIGRAGGIN
                     YYEYALGNPVRHFDPYGLKSEIVIWAPVGIGQSSFGHVSTDINGTTYSFGPNGMTTEP
                     TSVYRQRNSFRDSLGLDLNLTPEQENALMDCLGRSQPDYNFLTNNCGGPAQKCLNWLG
                     YDVGQLLLPSQISQSLVGMGLVRSTTFYPGATSTPSHPK"
BASE COUNT        10419 a        13895 c        14800 g        10187 t
ORIGIN      
        1 tgcaccatcc gcttcgcgga ggtccctgaa tccctgcgcc ccaaacatgg cggttgcggg
       61 ggcaggcgtt gtatcgatgt atcgcgaatc ggttgtgagg cggtcccctg ggtgaccggc
      121 ggggaggcgg aagtagacga gacgggtgtt gtcacaacga tcggagccat gggctgagtg
      181 ggttggctga cgttcgtcgt cgcggccgcg ccggttgccg gagcgttggt catactgacg
      241 gtcgcgaggg gggcggggct tggatcctgg agtggcacga ctggaacagc gggttgagtc
      301 ggaggaactg ccgtcagtgt gaggcgttca ggggctgccg gtggaggtgg cgcggtttcg
      361 gccggtgtga gacccaaaag ctctgccttc tgattctcca gctggacgat caattctttg
      421 agcagagtgg tgctctgtgc cgactccgtg cgattgccga ttcggagctt gtgattcttg
      481 tgggcctggt attccggcga cttttcaccg aatagtcgcc ggatcgattc ccggagggaa
      541 agttcggttc tggcgcggac tgcgtcacga tacgggaatc catctctgct caggtcatcg
      601 atctgtgagc tcagttcacg caacgtggtg atgccacggt tgagttcttc gacggcgttc
      661 tgtttcgatc ggctgcgttg cggttcgcta gctcttgctc gtgccatgtg tcctgtccct
      721 ctattgagaa aagtctatcc aagcaggtga tatctggcaa gaaatccctg tttccatccg
      781 tgagtccgga ggcgggaacg ggcggggcag gcggttagtc aacccactcg acatcgccgt
      841 tgccgggccg tggcgcattt ttctgatcgg gagcggcggg aggaccggct aggacctctc
      901 ggaccaacga caacagcgtt tcgcttgtaa aaggtttctt gagaaagggt aatcccgctc
      961 gtacgaggcc gcgatcttgt aactcagagc tcgggctgcc ggacatgagc aggacgcgga
     1021 tctcgcgctt atcttgcagg agccgctcaa ccatgtcgag accgttcacg cggggatagg
     1081 ggttcctgtc gacggaaagt tgaaagcccg gtggagggag aaagacatcg gcgatcacga
     1141 ggtggatcgg gtcggggtgt tcggcataga gcctgagcgc ttcggcactg cccggtgcct
     1201 gaagcaccgt gtagccggcg tcccgcaagg gtttggtact gacaagaagg gcgctggggt
     1261 cgtcgtcgac tattaggatc gtcgaaggct gtttgtgacc ggcggccatg gtggactcct
     1321 ctgttcggtg tcggtatccc gaggcctcag aaaggagggt gttgttcctt taagcgttcg
     1381 tactcgcgct gctcgcaact gccatgtttg gggcgcaggg attcagggac ctccgcgaag
     1441 cggatggtgc aatagcgctg ggcctggcgc cgccggtcga ggtcttgttg ttcttcgagt
     1501 tgctgaagtc gaacggtgag gtcgatggcg ggaagatcgt tgccggccgg attgtcctgg
     1561 cgtgatggga tcggttcatc ttcactggat ctggggtggg gtccgttcaa ccgccagcgt
     1621 gtcaacacat cgtcctgatt gaacacaatg atcaagccga actgggagta gtcccagatc
     1681 agttctccct gctcggggcg cgcccagatg gagcgaggca gaccgtatcg attccagatc
     1741 tcgtcacgca gcatgcccac ccgtggcgtg aggttccgga tcgccgtgat ctgcgcctgt
     1801 atcggagaaa tgtctctatg ttttccggga ctactgtgtc tggagagtga ttccagcgca
     1861 gtgcgcggca cccgcagcaa tcgccacacc gtgataagcc cctgcgcgca gtcagccgga
     1921 tgcaggtctt cgaaaatcat ctgcgtatcg aggggaaagc cgccgacctc ctgagtgcga
     1981 gtgaaatttt tgacctcttg gacggcggcg acgtatgcgc gttggcgccc gtcttctatg
     2041 ttaggggcgc atgaagctcg ccctgtaaag aatacgtcat ccccgaaatg aaagagcgct
     2101 tgctcggtcc aaaacggacg attctccgca gcatgtgtcg tggaggccac cgcaagactc
     2161 aataatgtga ggagaaggta gtgagccata tgcgcggccg gagcaaggac attgcggaac
     2221 ggcacccttg cagtatatcg cgtgaggcaa aaaatacgac aaaagagcga ttatgcacgt
     2281 ccagggattg tcggacagag attgtgagtc gggtccgggg gctggactgt cgagccaggg
     2341 cggcggggca acagctagta gttcatggag ggagtcaaat tctccctgcc cgacgaggcg
     2401 ggggcggcgt gggccttcgg cgggctcatt tcggtgctca tgaggccgga cccaggcggg
     2461 caaatgctct ccggcgcgac agcgccagga ttgcgcagac cccaggccgt tgcgagggca
     2521 tgacaggtga ggagactgac cccttcctcg atattgctga agcgttcggt cgattgagcc
     2581 ttgccgctcc attcgatttc gccggtattc acatctacgg ctctgagggc aaccgtggct
     2641 gaatagaccc gctggctgtg aggaaagccg ctactccaat ccagggcttc ccaggaaccc
     2701 acctcggcat tgctgaagac caccagccga gcgccgacaa gtttggccat ccgcagcaca
     2761 tccgtttcca ggtactggta gcccgtgagg ctcactttct ggtcggccgc cgcctgcaac
     2821 accttcgtct ggtccacgac gagcacgccc tttttgagca accatgtgct cgcactctga
     2881 accgtgtccg ggcgtccgcc ccagacgacc gccctggtgt agggggaggg gagtttggag
     2941 tgaaagccat ccgtcgtcgg ggtttcgatg aacgcgtagc cgtggcaacc cgttgttgcc
     3001 aaaaagagga gtgacaggca gatgaacaga ttcgccggtt tcatgggtct cttcggaaga
     3061 gcatgtgctg cgaacacgtc gggagcctaa tcaagagacc gtttggacga gggtagtata
     3121 catgaacaat tctacctacg tctgctctat tcgtcgagcg ggctccgttc tcgaagcagg
     3181 gcggggtccg ctacccgtac ctacttatgc gaatggcata gggttccatc ggcgaaccgg
     3241 tcgaggtgct agagcggttg gggtgaatgc cgaggcaggc ggcagggatg cggatcgtgc
     3301 ctggaaaggc ggcggttctg ttcacggctg acagaaccgc cgcggtctca caggatctcg
     3361 acctgcggaa aggcgttttg ctcgcctgtc ttctggggcc gatcagccat gatcatgagc
     3421 tggatggcaa tcaacgggag aagcgcgtaa ccgatcagga tcaacgggaa ctcaatcatg
     3481 gtgaaaactc ctttgttatg cggagcctcc agtgcaatct cgataccgtg tcatgatcta
     3541 gccctaacgc attgattctt atagccaccc aagcgcgtag cccaaaaacc aaggtgatct
     3601 ctagcaagaa tcgatgtaga gaactgtgca atgagaacat ttttcgctga gccggaaggc
     3661 ctaagggtag ctgtacaaaa gatagacgcc ggtctggcaa gcgcctctaa ttcggataga
     3721 aatcaacgtg caagttcatg tcgaaacggg ggggcgaaag cgggccctgc cggaagtccc
     3781 tgcgcgcaag gcgtgaggta caggtagaac aggagggtga gagctgcacg acttggagtg
     3841 agcgcgaaag aataggcgac cgcgtgagcg agtgggtgac tgagcagtgc aagttgaagt
     3901 gggatggaat ggtgcccccg acacgaattg aacgtgcgac ccgcggttta ggaaaccgct
     3961 gctctatcca actgagctac gggggcgttc gagatttttc agtaccttac accactgctt
     4021 ccggcgtctc aagcgccgaa tgggcctgac cgtgacaaaa acgtgacagc cgcccggttc
     4081 cccgccgatt gagcacctcg atcccccggc gcagcgactc cgtgcaatgg tgcgcgtagc
     4141 gttgcgtcat cttcgggtcc tgatgcccca ccaaccgttg gaccgtgtag ggatcgacgc
     4201 cgtcctggca gagccgggtc gcgaacgtgt gccgcaggtc atggaagcgg cagttctcga
     4261 ggctggccct ggtgagcgcc cggcgaaagg cccgattcac ggtgccgagg gtccggtgac
     4321 cgcccttgcg attggcgaag accacgaggc tcgcgtttcc tgagcggccc tggcgctggc
     4381 gcagcacgtt catcgcccgg ttactcaagg gaatggtccg cgggagcccg ttcttggtcg
     4441 tcgcggcttc cacggtgagc gtcccctggt tcagatcgac gtgcggccac tggagggtca
     4501 ggatctcgcc caaccggagt cccgtctcca ccgcgaatgc gacgaactca cggagccagg
     4561 ccggacaggc tgcgagcaac cgccgttctt cctcgtcccg caaccatcga tcccgtccct
     4621 tcggttcctt ttcccgagtc accatcgtga agggattgcg actggcccat tgccactctt
     4681 tcatcgcgag attgaaggcg tggcgcgcca tccccaattc ccgattcact gtggcaccac
     4741 tcgcccccgc ctgccggcgg gccgtcttgt aggcggcgat cgcggccggt gtcacatcaa
     4801 ccaagagcag gtgtccgaac gccttgacca gatgggtctt gaggctccgg tcccgccgat
     4861 gggcgcgtgg tttcttgttg agcgcgctgt gttccacgta gtacttctcc aacagctccg
     4921 ccatggtctt gtggtgctcg ggcagttccg gaaaataggc gccggtctcg atctccgtcc
     4981 ggaccttcgc gtagatccgc tcggccagct tctcgttcgc ggtgccggtg ctgagccggt
     5041 accgtttgcc cttgtgtgaa aacgacatcc accaatgttc actatctttt cgttggaaga
     5101 tccccatcgc tgtttctcct ttcgttgcga tgcaggtctc ccggacggcc cgagtgtagc
     5161 ggaaccgctc gggccgagac aaccggagag tcctgcgatc gtgctggtac gtgatgctgt
     5221 gcctgtctcg tgggactacg cctgaataag ccgtttcacc cgtgccagca ccgccggaac
     5281 atcggggtga tggccggagc gcctgggatt gggccgggat gagcgggcct gctcccgcac
     5341 cagacagccc tccatgaagg acgcgaggtc ctcgcgccgg aagcggaggg cgccctggat
     5401 cttcaaggtg gggatcgcct ggcgcgaagc ccacccgtag accgtcttgg gatggacgcg
     5461 aagccaggcg gccacgtctt gcacagtcag gagcgcacgg ggatcagatg cggatgattc
     5521 ttcagacatg ctctctctcc tttggtctcg cggggcgccg atgcgcctcc agctggttac
     5581 agtctcggtt catgatggct cgtgtccccg gcacctgatg ggacgccgtg agaggcttga
     5641 gcggtgcatt ctccggtggg agtggggagc ccgagcaatg ccgtcagggt ctgttcgagc
     5701 agcgaggcca agtgagacgc cgtgagaaag tcgtagggca cggaatgggt cgggccatac
     5761 ccgttgcatc cgtggatggc ccagcgcagg tggtctggat cagtggccgc ccagaaatag
     5821 atggccagcg cctgttcgtc gagacgtatg gcgatgtgcg agggatccgc atccaattcg
     5881 gtgagcaccg acgcggggac cccctcttgc cacagcacgt gggccacatg ccgcagcatc
     5941 gggccgatga cggtgtctac aatgacctga aaccgttgcc tcgcgagggt tggtgaggtc
     6001 acacgtcttc cgtgggcacg ggcctcacgg aggcggacac ggagtgaatc gaacggggga
     6061 taggtggtca tggcagtctc tcctggctag agggttcaga aggccggttg ggatggctcg
     6121 cgaccgcagc gtcccgagcg gtttggcgtc atgcgcaaac ggaacggggt agcgcggtca
     6181 gggcagcacc gcgagttcct caatccggat ggcgaggatc gcatgctctg tgggtgattt
     6241 ggctccggcg cggtagagat agtccatcga gcccaggatc ctgaggcggg ttccggcttc
     6301 cagcgtcgcc atccgttctg caagggagcc taaggcccag gcatggacca cataggtctg
     6361 tcgttccagc gtgccccacg tgctcggttg ccgtttccct tcgatcgcga tcagcgcgat
     6421 ggccaccaat tcgcccgtca ttcccgccag caggtccggg gggtgcagac agaggcccgt
     6481 gacggacacg gcattgagat cgggaaggga tggggactgg ctcgggtcac gatgcagatg
     6541 tggattcata acaacctcct gtgacgggtt catgttaggc agtgaacgac gggtggcccg
     6601 tccggagagg gaggagagcg ttatcgtgcg acgtcggttg cggaacgcga caagaaccga
     6661 aggaggtcct gcaatcggat ggcggccaca tccagtgcac cgggcagtcc gtccggtccg
     6721 gcagtcacat cgatgcgggc gagcagttcc cgtgtccgca cgttgaaaca ggacagggtg
     6781 atgttggaac gggtggcgtc tcgctgtccg gccagcacga gcagaacggt cgccgctcgc
     6841 caatggcccg gaccggccgc atggttgtct tcgacgatgg cttggagatt ttgttcgatc
     6901 tctcggcgta cgaggacctg ctgctcctcg gcggctacta cgggcaggtt ctgctggagg
     6961 agcagtcgct cgatcgtgct gcgcggatcg gggagcttcg gaggcagcgg atccatcgtg
     7021 agcttgaaca ccagcgagtg cggccaccgg ctgcggggcg ccatcacggg cacctccagc
     7081 cggacagcgg cttccaattg cccggcagcg atctccaggg gccatcgatg taacagacga
     7141 tcccgatgcc aggccgcgag ttcgatccgg ccatgagcta cagccgcaaa agcaatgtgg
     7201 tcttggcgta acgcagtggc caggtggctt aggcggatga ggactcgtcc gtcttcgtta
     7261 acggggtagg tgacgaggat ctcccgctcc ccacgccaag acaccgtcac gcgtccctct
     7321 gcgagaggac gggaatccgg ttccagcttg tgttcgacaa tcctgtcgac cgccgtcagc
     7381 tgtcggttcg agcgggcgat catgagggcc atcaggcagc cggtccaagt gatgtcgagc
     7441 cactcctgat gccgttcaag ctgacggtcg ggactcacaa tggtcgcgag gacattcaat
     7501 ccccatcccc agattgatga gggacaggcg aacaatccga cgggaagcgt caacggattg
     7561 aggtaatagg tatgctgctc ttcctgttgg gaccggatca cctcgacctg acgcatcagg
     7621 ggctgcgtca cacgcacggt ccagccttga tggtcggggt gcggcagaag ggtggcttca
     7681 ggtgtgccgt gcgacgtgac gccagtttct tgccggacgc ttgagcccgg gataattcgg
     7741 tctgtgaccg tgacacaagc ggattggacg agtagaagcc cccacaagag cgaaagacac
     7801 gaggcatggc cagtccagca ccttgtgctc aaccgggatg gatcctgcgc atcagtcata
     7861 ttcagcctca caagtggatt gcatgtgagg aaaggggatg gctcggcgct gccgaacagg
     7921 tcctatcggt gagattgaat gatgacgggt agagaggggt gtggattgcg atcgaaaatt
     7981 cgaatgacac ggtcaaggtg atggccgcag tccttctctg gatggggcgc ggctcacacg
     8041 tgagagcgat ggtgcaagaa gcgaggtggg tattgtgtgc acgggtggtc ggagccttag
     8101 cggttggaac tgcgcttcct cacgctggcg cggagtttgt ctaacacatc caacagggga
     8161 atctgtttgt ctatatgtgg gccgcctgga ccggtggccg ttttaaatcc gcgcgcgatc
     8221 tccgatggcg agcatacaag gagtcgcgct gcctgcgtgc gagcaatgtt cttgagtgaa
     8281 tccggaatgt attcctcgcg ctgcatgagt aaatactcca ggaatgaaag tgtatgcaca
     8341 tagcgctgga tgctcgtgag cgggttggag tgccccatga tacggctgag cacatggaac
     8401 ggattcgtgt cccagggatg gtggatgtcg tcatccagat ggggaagcga aacgaagagt
     8461 tgacggaatt gtgcgaggta ccttttctca aacagcgaat gggcaaagtt gaagcccagc
     8521 gtgttgctcg ggagttgatg gaacgcggca taccatacca gtggaaacca cgatgcgcct
     8581 tgatgacgca agggatgcaa ggactgtacg tcgagcccgg ctcgactcat cgtctggctg
     8641 atgcgatcgc cgagttctga ttctgtgaag gggactcctt gttcgtcgac cagcaaaggg
     8701 gccattccat tgaaagatcc ctcctcctcg cgcgaggccc ggtaggccaa gacggtctgg
     8761 atgtggcgat ctgggatgag cgcccatagg ggcacccggc gaatccctgc gcgggtcttg
     8821 cttctccgga tcgacaggtg cagatctttt gctgtgccgg aaaggtcgtt gaccagcaag
     8881 cgagcgagtt cgcctcggcg agccattcca tagccgccaa gaatgatcgc gatcttgtcg
     8941 ggcggggaca cgtcatcaat cagggcatcg aactcatggg gcgaaatcag cgggaatgga
     9001 tggatctcgc taccgaccca caatgatttg ttgctgaggt tcaccacggc cacgccatgg
     9061 ttgaagtgga gaaatgcaaa tagacgaacg aaatgggctc gttgagtctt cagggtttct
     9121 ccggcaaggt ccgtatccaa catgccgctg accaccttcg ccacgtcgtc ctctgtggcg
     9181 aggttgagca cattgcgagc ccccagggtt tgaacaagtc gcgcttgaat cgtgcagtaa
     9241 tagttccgca cggaaatgga tgaaagtggg tcctgtcgag tggtgaggag gtctctgagc
     9301 cattcaagtt ggaggcggaa attcgacagc ggtgcccatt gcgaatatgc ctccgttggt
     9361 ggcagcaatg ggagatcttg ggcaaattct tcaatcctct tcgcgagtat gttgcgttcc
     9421 cccatgggtg tggtcttact gatcacgcgc agcagcgcat tgactcggtg ctgcaatggc
     9481 tgcaactgcg cgcgacatgc gtcaactgtg gcgtcctgct gacggtgttg ttcttcggtt
     9541 agcggaaggg tgctgcttgc agggctggca tatgcgaccg agggggccgg caacaagagt
     9601 tgcggagttg gcccaagcgc gggctttggt tgcttggttg ttcgaggtgg attgagattg
     9661 gggggccgct tgcctaagac gactttgtac catgactcgg tggtcggagg gcgtgtcacc
     9721 agcaagcggc tgcggagtgc cgtaaaaatg ggtcccacgg tcgttaccct atgagcgcga
     9781 cgtgcatcga tgatcttccc gagcagcgac ccacttcgtg tcgattcttc aactctgaga
     9841 gcaaggtcag ccgaaatctg atgcgggcgg aacttgatgg gatagcgctt gagccattct
     9901 ataaagaagt ccgggtgaaa gcggatgtat tcatggaaga tgtgcgagcg cgggtcgtct
     9961 aggactaagg gttcatcaac atgacgcccc cataataagc gcatccggat gtatcgtaga
    10021 agcaagagtt gggcgagagg gggaagccag aatgactccg ttaaaaagcc aggaggggtg
    10081 ttctcctgat tctggcccga accttttttg tagcgtcgtg gcacccaagg aatctggagg
    10141 aatccctctt caatacacaa atcagtcaca cagatcctgg tgagcggggc aatctgatgt
    10201 gaaaaaagca ggtcaccctc cataatgaga ccaagcagtg caatacaggc ctcatcgtgg
    10261 agggcgagcc ggagacgatc ggcgccgtcg atactcggtt tcggtaattc cgtatcaaac
    10321 actgtcttac ggatcttttg ccaccattcg tccgtgcgtg accagagcct gacttcgtcc
    10381 gggtgaaacg acgcggctag atcgcgtttg gtgtccaacc gtattcgagg tattggaccg
    10441 tggatcagtt gcaagtcctg gagatgatgc agcaggtagg cgagggcata ctcaggcagc
    10501 gtacttttgc gtttgatgtc cttgcatttc tcctgaaggg tcgaaatgga tttaggagag
    10561 agttccactg gaagagaaac cgacgccgag ctctctacgg tcgatttcaa atggctcagg
    10621 atggcgaaac tcacgctcat gactaggtcg ttaagaaaat gatgatggcc cagaagaatg
    10681 ggacggaggg tatccagagt gccggcgacc atcgacataa atcgaaatgg agataagcca
    10741 atcattggag atgtactcat cgacgccatg tgaagctgat tggggagatg tcaaggatct
    10801 caagcatcgt atcaatttct ccggttaaca catcgtaagc gctatcgaga gcgctgactg
    10861 agactcgccc catccattcc cggccttggt gctgatggcc caggatcgca tggagaagac
    10921 gagatggaag gcgctgccag aggtgagacc ggaagaagtg ccggttgccg tttaatggca
    10981 cccgatagcg gttgccgagg ccaatgtctt caagatgctg acggatggtt ttcggggtca
    11041 gtagtcgaat ccggaaatca ggaaggaata ggaaaaaaat gggccattct tcctgcgcga
    11101 gatcgtcata acgatgtatt cgccgcatgg ctaagtagac aataaacatc tgtcttgccc
    11161 ggagcacttg ggtgataagc gacttggcaa cggggcttag cggaataaag cgactgtcat
    11221 cgtagcgttg attggctttg tccgcgacga ggaccaagtc tgtgtctacg gagtcgtgcc
    11281 tgacgtggtg atcccgtaaa ttggcatcgc tcaccggcct gatggcgctc gcgtacccga
    11341 aggcgagaaa agaataaatc atgaagtgat tgaagtactg gcggcggtct tggagggatg
    11401 ttgccgctgc gagatcagcg aggcaattgc gataaccctg gaagaatgac gttacggtgt
    11461 cgatgcgtgg caccgctttc gaaccgaacg cggcagatgc ggacacgatc tcatccggag
    11521 gccggggggc tccctcaact agcgtgcgca gaacagtggt cgcgtcgtct gctggtagac
    11581 gactggcagt gagttcttgg agcagggctc gagcatattg cgtggaagcg tcccagtaat
    11641 gttgtgcgag gtcggcaaga cggactctgg tgtaaaagac cgcggttctt aagaagtagg
    11701 gcactcgacc ggaaatatag catgaaagga tttcgttgag tgcggagcgt ccttcgtaca
    11761 gcagcttgaa ggcgcgagac aaagaccgga gtgtaactga ttcgctcgtt gtgatctcat
    11821 cgcgaagaaa acggttgacg tcggctaccg atagccctct tggtggattg ttgctgcgga
    11881 ggaagaagaa atcggcgaac tctgttcccg cgagggcaga atcatgggtc agacgatggt
    11941 gcagataggg ttcgatccct ttccagatga ggggtggaag cggaatagta atgtctctgg
    12001 ttgtctcctc gcacccatct agactgatca gaaggtggtc gctgttaaac ccgcaccact
    12061 ctagcggccg tacatatcgg agtgtccgtg tcgttctatc aagaaacatg ccctcgggag
    12121 gtatgccctt gttagttctg agcagtttga gtcccaatgc gctttcagga ttgagtccgg
    12181 tgtgtttgac gagccaggca acggcggctt caggttgccc acactggacg agatgccaga
    12241 gttgcgcatc gatcgacagt gtgggacaag accgttccca ccaatacgca atgctcgagt
    12301 ggatccgagc gtctcttgcg agggcccgat gatgaatcca agtcaagagg tcagtgacgg
    12361 gaaggtctgc ttctggggct gtggcggaaa gtttggaatg ttcctcagcg gactcaccct
    12421 cctgcagggc tgaggttcta gtggattcat gcgaatgatg gaaggttttt gtgccatcgg
    12481 ttgactcctc gtcatcatcg ttgaacttct tgtttgatgg ctcgggaagg tccccgcaca
    12541 ttacctcatc gtcggcaact cgggtgctga gacgggcatc tacgggtgtc agcagctggt
    12601 ttccaaacgc tagtcgcact gttcgggcaa aatggtcaac cgtggcctcg accggggatg
    12661 tgccatgtgt tttgccacga cccctactct gaaaattagc gaccgcggcg gcgatgtagg
    12721 gactgccatg cgcaggtaca aactggtgtg ctggggcgat ggccatcgga acaaattgga
    12781 ggaatcttcc caggtgtagg gaaaggtcgt atttgcgaga tctgtttata gtcgtgtctt
    12841 cacgaggcgt ggccaagatt cggtcatgac agaaaggacc aaaatattgg aaggcttcat
    12901 tcttcgggca ggccagtaga aaatggtaaa cggggtgctg tcgacgcgtg ataggagtag
    12961 gacgttgacc ggagcggagg attgctcgga tcagcaaacg gctcattacg attaatattc
    13021 gagcttccga ggcaggcata ttgcgatccc ggcagaatcg catcagaagg aacgcgagag
    13081 gtttcttcaa ttgctggagg ggggccttca ctttaggaat tcgaggcggt ttcggtggcg
    13141 cttccatgaa atcaagaagg aactcaaagg ctgcctggca tgctggacgt agcgagctgg
    13201 ggaggtgtgg aaggtaggtc aggagatggc tcgcgtcgtg cttggtcagg ccaaggggtt
    13261 ggtaagagat tcggagttcc tggagacggc tgatcagccc agccaaagac tctgatgctt
    13321 tcacgtggtt gtgcatggtt cgtccgccgt gacgggcttg ccgcggccag cgcgagtggt
    13381 cgaagcagaa acttgcatca cttcatagat cttggagagc gtcaatgact tgaagatcaa
    13441 tggcgccaag ccgacggata ccaaaacgtt gaccaaacgg cgtcgcacct ccggtctgag
    13501 aaaagagggg tcttccctca agctttcaaa ggcacggcga aacgagcgca gaatggcttg
    13561 atcccagggc ccgtttgtgg acgatttcgc gaccaggaaa ggtacgttcc tgtagccgtg
    13621 tgcttgaagt tcagcgaggc ggcgaacgga atcaacgtac aggtatttgt ttgggccaag
    13681 ctctagacac cgcaatggcc ggcccttgag gtcctcctgt agaagctctt catcgaagta
    13741 ttccgaaggt aagaccaaat ccctagtccc caagccgatg gatggcgcga cgtttggcgt
    13801 aatctctttg gaaagaccac gaggctttaa cgtaggagtg gtagcgatga tacttgctag
    13861 gaattcggcc atagggtgaa cgaacaagtt agcttctgaa agcgttagcc tgcgctgact
    13921 gactcgtatc ggctctttat ccggtttaat tgagatgggg caggcaatgg gatgttccgc
    13981 agatgggtcc caaatgaacc cgatgatctg ctcaaagtag acccggagct cctcgctcac
    14041 cgccctttcg atcaaaaggg ttttcaatcc atccccggtg gggggataaa tctgctgccc
    14101 ttcgcgttcg cgggtcgtct tgtgtgaact gagctgcatg aaatccttgg ccaatggtcc
    14161 gtttacattt ctggcaatat cgtacgttca tggtatcgct gaatatttgc aagatgttga
    14221 tattcatggt gaattcacat gccttcatct tctctcacct gcatgatgtg agataactga
    14281 tgctgaggtt gccgctaggg taaccctgac ggcttaagga gtgacctggt ttggccgaga
    14341 gtatgacggc gcttggaatg gcaaggttgg gtaggtgatg aagaagaagg agctaattct
    14401 taaggaactc gaccatctaa tcgagaccgc ctatttcaaa acgaaaccgc tccagagaaa
    14461 gtttctacag catttttccg ccgtatatag gacatccgat gagatcgtta cagtaaggaa
    14521 ccaagattta gttgcgatct atgagaacga aacacctgaa atcaaaaatg atgggaacaa
    14581 attcctcagc aaaaagaatg cggtgtccaa gcacattggt aagcttcggg agaatctgaa
    14641 ggagttctat caaggacctg gcaaagcaag gcagcatgta atttctattc ccaaaaacga
    14701 acatggaaat tactcccttg atgttctgag tcgtcagagc aagaagggta tcagagaagg
    14761 gcctccgaag gcggtacaag gcaacgttct tgttctatcg gatgccaaca atacaggttc
    14821 tgttcttcgc ccacagtctg ttaccgagat catccttctt gttctcaaag gcgtggatag
    14881 aaagcgtttg gtccagattc agagggcagc ggcaggaata atgtggggtg tgttgttggt
    14941 tgggctagcc gtcctcatcg tcttgctgat aggtattcca tactggccag ccatgccatt
    15001 gatgggtctc tccctggcgg gattgctgat gatctgtacc tgtatagccg ttcttgcaag
    15061 gccaataact cgattgtcgg acatgccttt ccgtcgatgg tggggagata cattctgcat
    15121 gtatcggcga gaagcattgg ttttggctcg gtattcgggg cgctgtatgg tggacggatg
    15181 ctatgggcac ttgtcggttg ttcaggaggg gcatgagcga accgatttat tttatccgct
    15241 gcccaggcgc tggattatcg tctgcaagaa cgttccgacg gagcatcctc gcatgccttt
    15301 tgatttcacg gcactcgatc gggctcaggc tccaaacggt tcttccgaac gcgaattagc
    15361 ccgcagggcg tgattcttcc ctagtttctc ctgcagctct cctgaataaa cgactcgagc
    15421 ccacgaagtc ccagtagtaa aggggcttat tgggaggata ctgcctcctg gctgaagatg
    15481 attttccagc gggaaacggg tattcttccg ccaactaata gccttccaga taggtcatct
    15541 tatccataac gcaaagcttg aatgaataac gactttttct cgaagccgat cctgaactct
    15601 ccctacgcga tttcctctcg tcattgggag cttgatgaag gcggccagcc aacgcagaag
    15661 attatcgaat ctcgccggcc tgccgaattc atcacgccga ttccaaagcc caagaaacga
    15721 aaatcctcag agaaacgagc taccgtcaag aggtgtttaa atggggttca ggcggcggtc
    15781 ctccccggtt gatggctcac gtcgcccgtc acgctttctg acttcatcat cacccatcct
    15841 tgaacgtcag gccggaggtg accagcggca gcagctccgg cgcgttcagc ttcctccacg
    15901 acgtctcggc gatccggagc atcttccaga tgatggcctt ggctccctcc acgcgcttgt
    15961 agcggcggga cgcgttcgtg cggagacgca ccgaggcgaa aggggattcc acgatgttcg
    16021 tcgtccgtag atggcgccag tgctcctgtg ggaacgagta gaacgtgacc atacggtccc
    16081 agtcccgcag cagcgtgtcg acggctttcc cctccgtccg ccggttgttc aacgtcaatt
    16141 agatttgacc ccttcaaatc taattgacgt tgaacacacg agcaaggaat tgcggaccgc
    16201 acagatcgcc atctggcact accaccccat caacagccgg ttggccatcc tgggctatgc
    16261 cgacgaatat gggtgtaaag ggccgcccgg cggttcggct tggatcgcaa aacggtgaga
    16321 atttggcatc gccgctggtt ggccagtggg ccggcggggt tggtgccacg acatccgcgc
    16381 acacgacgcc ggcgcatttc tgacgaggcg gtgcgcttga tcgaacaggc gtgccgcgag
    16441 ctgcactttg gcgctatgcg aacccggttc tagctggacc gagtgcatca catccgcgtg
    16501 gcggccgcga cggtccgtga gtctgccggg acttcggcta tccgccgatt cggcgcactg
    16561 ggccgcgaca ccctcggccg ccactcctct ttagcaagga gcagccggtg actgtgtgca
    16621 ggtcgattca aggaagtgaa ggtcgttggc cagaaatgtt tccaatacac gtcgcttgat
    16681 gactgcacgc gctatcgcct cttgcgcctc tatccgcgca agtaccacgg tacgacacgc
    16741 cctcccattt cccattcgca aagttcaggt cgacaacggg accgaattcc cgctcgcctt
    16801 tgccctggcg gtacaggagg ccgggatccg cctgcgacac atcaagccgc ggtggcctga
    16861 acagaacggg aaggtggaac ggagtcatcg catcgacgag gaagaatttg gagttgctcg
    16921 acgtttgacg agttcacatc ctcggcccag gcgctgcgcc cctgggaaca tcggtacaac
    16981 cacgaccgtt tctccatggc cctgcaagtc ctgacgcccg cggataaatt ggcaacgttt
    17041 ttctcgccac cggcaccatc atcaccaccc acgccatgta cagacaccgg ggccgctgct
    17101 tgacaaatca ttgcacacaa agaattccag tgtgtctgtc ccccagcatc tacttttgat
    17161 ccccgtgctc acctcatttc tctggtgacg acttcagatg gagttggcct cggctggtgt
    17221 cgagggtgat ctgatacgca ccgagaaagc tcaatcccaa gagcccatca accgtaaatg
    17281 gaagatcggg aaggtcgtga atcaagacct ggctattccg gatctctgcc ccgccgatcc
    17341 gaatcgagtc aagaattacc gcatccgccc tgactgagcc acttgcggtg tggagcaaga
    17401 cgtgagtcgt gtgggctgtc gagtaaaggc cgagatccaa ggcgagtcgt tgcgagatga
    17461 tcgtctggga cgcaccggtg tcgacgatga gccgtgcttg tttggagccg ttcaaggtgg
    17521 ccgacacgac gagaatatta cccaggcgtt cgaccgggac ggcggtagtc agtggaccac
    17581 cgagtacagg tgccgtgggg agggagtctc cggtatcctg gccagacccc gatgtcgaag
    17641 gtgcactggc aggggctgaa ggtgctgttg aggtcttgag ggaagaaaag tccaaggcgt
    17701 gacaggccag aagttgacgg ggactgtctg tgaacaccgt cttcccatgg gcatccacgc
    17761 actcaaagag ggtgccggtc gcaaaggtat ccggcactac gagcagtatc agcgtcgaga
    17821 gtgtgaggaa tttcgcctgt gccacttgcg atgtggggag ggcacaggct ggaatttgat
    17881 tagaccgcca ggtcatgaat gtcctggccc acggcaggag tggttgtatt cgagagccac
    17941 gcgaccaggt tgtgatcacc caacagtcgg ctgaccacgg cagttcgggt agtcacccga
    18001 gttttcttca tcaaccgttt cagatgatcc ttcaccgtgt gctcgctgat cctcagtttg
    18061 ttcgcaattt ctttgttcgt aaagccttcc aagaggtaac gcgtgacaga tttctctcgt
    18121 ggggtcaggt ggatcggggt tggtgggggc gggtgcggag cgagcgcttg atacgttggc
    18181 tgagccggct caatcaggag caggagttgt ccgtcacctt cattgagtgg cgcagggacg
    18241 ccaaatgcac ggaggagaat cggacgagag gtggagaagg ttacgcgtga gaggcgcaat
    18301 ccctcccagg ctgctggctc gggagacgct ggcagcgtct gcatcacttc ctggcagaat
    18361 gatcgaattg cgggaggcag gtcagtagat ggcattggac gaagggtgaa ctcctgaagc
    18421 aatacgcgag cttccggatt gaggtacagc agttgaaagg aggaggatag taggagcagg
    18481 ccggcttgac tggtgggatt cagctctttg atgtccgcat tcgaagaact aggttcctgt
    18541 gcaaccatca tatgttccct cgccgtaaat ccaggttgct gttgtctgtg ccggaggaga
    18601 cgcattcggg cgagtgtact gacggcacga gcaacgctca accccaccta cggtggccct
    18661 cccccttctg agtgagcagc ttgtgatcgc tcacgagcga gtagagccat gctggaatcc
    18721 gttgaaggca tgctttcttt accccgaatt gttgagccga cgggaatccc ctccctctcc
    18781 gttagctgat cttacccctg tgaacacatg cccccagatg ggtgtacatc catctggggt
    18841 gattgtggtt gcggaacggc tttccgtaac ctctgtcgcg atgcagcttc cgtgtgtata
    18901 ggcaacgtta ccccgcagca aggagcttga catgcgtctc gatcgtcgtg ttttgccagc
    18961 tctgacattc gtcacggtct tcatggcgtc aatcagcctc agcacaattg cacttgcgaa
    19021 agaggcagat actgaaatgc ctatcggcgt gccgggtggg gctgtccacg cagatccatt
    19081 cacaggtgtg gcgacgatga gtattccaat cgaagttccg cctggtcggc atggcgtaca
    19141 gccgaacttg gcgctgacct attcgagcag caatgggaat ggatgggtgg gtatgggttg
    19201 gaagctggag ctgggtgcga tcgagcgtca aacacgcttt ggagtggatt attcaaaaga
    19261 tgattacgtg atccgattgg atgcaatgtc agctgacctt gctcccgcgc cgcctccggc
    19321 tctaagtgac gaatatcgtg cgaaggtcga aggcgggttc ttgcgtgtca agcacctcgc
    19381 gaatggagga tgggagctca cagataagaa tggaacgaaa tacttattcg gaacctcatc
    19441 gatgtcacgg atgtatgacc ccggggattc tacgagaatt ttcatgtggg gattggaaag
    19501 ggtagaggac cgagacggaa actacatgac agtcacctat cagaccgatg gcacgacaaa
    19561 tcagcgctac cctgaccata tcgactatac ggggagtggc accctaacgc cgtctggtgt
    19621 ggttaagttc catctggaaa ctcggccaga tagagccacg ttgatgtata caagcaagta
    19681 tcccgtgcgg acggagcgac ggcttaagag tgtgacagtt tggtcggatc agtccatgtc
    19741 aaaattagta cgggcttatc gtttgggcta tgatcttagc tatggctctg gggcctccat
    19801 tttggttggg gtcgaacaat tcggaagcga tgcaattatt gatggtactg gggcgatcat
    19861 caatgcgtcc agcgcatcca aattaccgat tgtcaacttt aggtacagac aagaatcgca
    19921 aacgttcgcg caaggtgtcg cgtcattaag cggctggtgc tcgggtggtt cccagttcaa
    19981 cgccagtgac ttcaatgggg atggcaaaca agatctgtgg tgccgaaaca caagcaatgg
    20041 gttttctatc gccatttccg atggggtagg gacactcact gccgggagta atgtttttag
    20101 tgggtgctca gttcaaagag ttggcgactt caatggtgac gggaagatgg atcttggatg
    20161 tgtgagctgg tatgaacaac ctcggaattg tggtccaata ggcgggctca catgtgagcc
    20221 attaataaac tggcgagctt caattgcgac tgctaccgat ggattaggaa ctttctctaa
    20281 tccatcgaac tggctggatc caatttgtgg ggctcttccc tcatatactc ctgcactggg
    20341 tataggagat tttaatggcg atggcaagca agacctctgg tgtcaaattc gaggtgggac
    20401 ctttgcgcaa gtaatggtag ctctttctac aggcgcagca ttccagacat ctagtaattg
    20461 gatatcgaat tggtgtatcg gcagcacgcc ctttggggtt ggagatgtca atggcgacgg
    20521 caaaacggac gtgtggtgcc atggcggggg caatacctcg gtcgctcttt cgaatggaac
    20581 caattcgttc actggcggca atgtgtggtt gagtaattgg tgtggggatg gtctgccctt
    20641 cgggctcgca gattttaatg gcgatgggct caccgacatt tggtgccata gtggaggcaa
    20701 tacatcggtt gctttgtcca acggcacgaa tgcctttgtc gatggcggcg tctggcggag
    20761 cggttggtgc agcaacggag ttccctccgg cggcagtgat tttaatggcg atggcaaaga
    20821 cgatctgtgg tgttatacct ccggtggtac gatacaaatt gccttgtcta gcagcgtcgt
    20881 tgttcctgat gtcccctcat ggctaggcat ttggtgtgca acggggattt tcggcgtagg
    20941 agattttact ggtgacggaa aacatgacct gttctgtcag gagagcgggc aggtgtccat
    21001 cgcacggtct gggtcttacc ccggtgctgt cgatttgctg gtgatagttt cgaacggact
    21061 gaccggtact agcacccttt cctacactcc ctcgacacag tacagtaata cgcagttgcc
    21121 ctatccgttt caaacagtct cttcgattac cacctgtgac aattgggtga gtgcgacgga
    21181 aacgtgtgca ggtttatcgg ccacgacgaa ctatacatat gccggtggat atcatcatcc
    21241 cgcggagcga gacttccgcg gctttaacta tgccaaagtg accggccccg tcgggccggc
    21301 aggcgagcaa gccatcacgg agacctggtt tcatcaaggc aacgatgtcg ccgtgggggc
    21361 gaacaatccc gcggcgccga atggctacac caaaggcctg ccctatcgca gtaaggtcac
    21421 cgatgcgact ggcaaggtct acagcgagac caccaccacc tatgtcgccg atgcggacgg
    21481 cgtcgcgccc ttctttaccc ccgtggcgca agtggatacg tccattgata acggggccaa
    21541 acaaacccgt gtggtttttc caatcacttc cgggtatgcc cccgcctacg acgattatgg
    21601 caatgtgatg atggaatatc accatggtga ccttgcaacg acgagtgacg accgctcgat
    21661 ctatcgcgtc tttggggtga ataccactga ctggattgtg gggcttcaga ctcgcgagtc
    21721 tgtctatacc ggcattggga cggcttcggt gaacatggtt gctcagaccg atctctatta
    21781 cgacggcacg acgagctgtg ccaccgcgtc ggtgaatcaa gtgccgacca aagggcatgt
    21841 cacccggacg gttcgctggc tcaacggcgg caccagtccc gagaccagga tggcctacga
    21901 tgccttcggc aacctaacct gcacccgcga cgccttgggc caaaccagca cggtgaccta
    21961 cgacgcgagc aacaccttcg ccaaaacggc caccaatcca ctcggccatg tggccaccac
    22021 ggtctactac ggcgtcgatg gcgtactgat ggacaagggg ctctatggcc aggtcaagag
    22081 cgtgaccgat ccgaataatc aaacaacgac cagcgaatac gacgccctcg gacgcaagag
    22141 caagaccacg actcccgatg gcctgatcac cacggccacc tacaattacg gcacgggctt
    22201 caccgtcggc acccagcatg tgttgacgag cacgtccggg gctggactcg caacgatact
    22261 caccaccgcc acctacttcg atggcctcgg ccggacgatc aaaaaagaat cgaccggacc
    22321 ggatggcaag accgtcgtca cagaagtgca atacgacagc cgcggggcgg ttcggcagaa
    22381 gagttcgccc tatttcaaga cgctcgagtc cgtcaccggt cgctgggcca tcacgaatta
    22441 cgatgcgctc gggcgtgtcg tgcgcataga tagacccgat agtacccgtg gcctggcctg
    22501 cttcaccgat tgggtcaccg ttaccatcga tgccgccgac catcgcaagc gcgagaccaa
    22561 ggacgcctat ggccggaccg ttcgtgtaga tgaatatcaa agcaccgtca gcacctgcga
    22621 taccaccgtc ggcaccccct atgccacgac gacctatcaa tacgatgttc agggcaatct
    22681 gctggcggtg acggatgcca aaggcaacat ctcgaccatg acctacgata cgttggggcg
    22741 caagacggcc atgcatgacc ccgacatggg taactggagc tatctctacg atgcggccgg
    22801 caacctgacg aaacagacgg atgccaaggg gcaggtgctg tggttccagt acgatgcgct
    22861 caaccgtcgg gtgcagaagg acttcgctac gcaaaagacc ttcggagccg gcgatgtccg
    22921 ctacacctat gacgggacga ccaataaccg gaagggccga ctgcaacagg tggtcgatgc
    22981 ctccggaacc gtcgcgtttc agtatgacag tgtggggcgg atcctccaga ccgagaagag
    23041 cctggatggc acgacctata ccacacagag tacctatgat gggctcggcc gactcttgac
    23101 cgtgacctat ccgagtatcc cttcgaagac catcagctat gcctacaatg ggccggtgct
    23161 cgacaaagtc tttgagggca cgaccaccta catccgctac acgaactaca atgccttggg
    23221 tcaggccggc accacgacct atggcaacgg cgtcagcacg acgaagacct atgccaatac
    23281 agctaacgcg gtctgcactc agcagaactt ccgactgtgt accctaaaga cgagtggccc
    23341 gggatcgggt ggcgggagcg gaagcaccgg cactctcagc ggcagtttca ccgcgacccc
    23401 gtcgacggtg aacctctcta ccgaaggcac gacggattgg gcccattggg gcctggtgga
    23461 tgcggcgagc ttcgaccata agagtggcgt gtccagccag ctgaacactt actctaccct
    23521 cggcggcggg acagcccttc ggtatgacga caatggcgat ggctatacct ggacgggcgg
    23581 tacaccgacg gccagtgcga ccaattcgac cactggcgtc tatgtcgtcg gactgaataa
    23641 gggtttccag attgtcgtac ctgccgatac tacatcgcgc accgtgaaac tctatctggg
    23701 aggatgggcg gcacaggcga aactagaggc gacgctcagc gatagcagtg cggctgccta
    23761 tactgaaacg tccttcaacg atgcgggcgt gaacggtacg aaccgggtgt atacgctcac
    23821 ctatcaggct gcgtcggcga gccaaacgct cacggtaaag tggaccgtga cggggatgaa
    23881 cgacccgcag tggggaaacg ttagcgctca agccgtcacg ttagtcatca acagcgggag
    23941 tggtggaggc ggtggaggaa gctcgacgac ctacaacgcc caagcggact tctccatcac
    24001 gcaaggccag cagggctggt actacctcag ttccaccggc gcccaactga cctggaacgg
    24061 gaacttctgg gccggcaccg atggctacat cggtctctgg aacgacggcg gctatccggg
    24121 caacagcacg gactcggtcc gtcgctgggt ggccccggcg gccggcagcg tccagatcac
    24181 cggccaggcg attctcgccg aaacttgcgg cggggacggc accaccgtca cggtcaagaa
    24241 gaacggcacg gtcctctgga ccgaggccct gccggtgcac acggtggtcc atagcttcag
    24301 cctctccaat acagtcgtcg cgaatgatca gctcgatttc gtgactaacc ggaccggcac
    24361 cagcttctgc gacaatacga tctttacgcc gaccattgtc ctgacgacag gaagtggtgg
    24421 aggcagtagc ggcacggcct atcaagatct ccgatatgtc tacacgccgg atggcaatgt
    24481 cagtgacatc tacgacaacc ttgtggcagc cggggccggg gatcaacatc tgagctacga
    24541 cagtctgagt cggctgacct tggccaacgg cccctatgga accagcggaa ccaatgcatc
    24601 attgacctac acctacgatg agttaggcaa tctcacgttt aacactcagg tgggcaccta
    24661 cacttatccc accagtggag cttccagcgt gaggccccat gccgtgacca tggctggcag
    24721 caacacctat agctatgatg ccaatggcaa tctgagcagc ggggcaggac ggaccctgac
    24781 gtacaacctt gagaacaaac cactgacgat cacgagcgcg ggacagacga ccacgtttgt
    24841 ctatgatggc gatggcgggc gggtcaagaa gatcgcggga accacgacca cccgctacat
    24901 tagcaagctc tatgagtgtg ataacgccag ttgctcacga atgatctttg cagggagcgc
    24961 gcgtattgct accatcggct ccggtgggat tgtctactac catccagatc atttgggctc
    25021 atcaagtgtg atcaccgaca gtgtgggcgc caaactgcaa gccctgactt atttccctta
    25081 cggcgccaca cgcactaaca ctccctcggg caccccaacc atagatgtgc cctataaata
    25141 cacagggaag gagctcgata gcagcacgag tctctactac tatgaggcgc ggtattatga
    25201 tcctacacta gggcgctttc tgagttcaga tacgattgtg ccgagtcctc tgaatcccca
    25261 ggatttcaat cgttatactt acgccgggaa caatcccttc cgatatacgg atccgacagg
    25321 acactttaag attggtaagt ttctggatcg agtgttcgac aaaccatggg cacgggcaat
    25381 tggatgggcg atcaatccga tgatcatgca attcctcgat cctgcaacac gcagaactac
    25441 ggttccagtt gccgcaggtg ttgtcggaaa tttcgtctgc gggcctattt gtggcggtat
    25501 ggcatcgggg gctacccgtg cggcactggt tcaatcagaa gggaggagag ccaacatctt
    25561 gcggtctacg ttggctggtg gggtaggagg cttggctgga ggcgtggcca atctagcggt
    25621 gcctggggat atggtgatgg ccggactcgc aggcggcatg gccaacgggg cggtcaacgc
    25681 tgctatcggg ggagggagtg tcctccaggg cgcgctgatg gggggggcca caggagcgtt
    25741 gactggctac tataactcgc aaggaatcat tgaccgcacc accgggccta tctggagtgg
    25801 tccaaatacg ctgataggta caggatattt actcctgaac acgatagcag ctggaccggt
    25861 catgttaatg ggcggtgaag gcatgggata tgcgtatggc aatggtggct ttcagttctg
    25921 gaatgctcca ttaatgatgg gtgggattcg cttgggagcc attaccttaa tgggcggagt
    25981 ggacggcaac ggcattcctt ttacgcctga ttataatgga ccgagtccat acgagggcca
    26041 ccagcagatt aatctaggac gacacgaggg tgggcatggt attcaacaag aagttctagg
    26101 accactgttc atccctcttt atttcctgac tggtggaccg catgaggcta atccgttcga
    26161 aatcggagcc gacaattact cgaccggcaa tgggtcccca tggtctggct tctagtagag
    26221 gatactgtga agaacatgca cttcctgaga ttagtcgcta cagcagggct attggtcctg
    26281 cctagctgtg atttaatgta cagtccggtg ctgtgtaacg gttataatat gccggtcgtg
    26341 gtcacgtctg tattcggaaa taatgctcga aaacgtataa cgaaaatatc atcaggagaa
    26401 tgtaaggcgg aggatcccat tgtgggagca ccaagaagtg attcaaatac tctgcgtaat
    26461 gaagtaaccg ttgaggctgt gacaggcgag atcgttgccc gatattctga gcaagtaaat
    26521 gaatatgccc gttctggtcg aagtttcact cctcattggt tactgtctga taaagggtta
    26581 ttttttattc ctcgaaaatt tgaaaacaat tggcaaggga atattcccgt catagagaaa
    26641 actggtcaat tttttattcc aaatcagctc aaagttcagt agcggttcct ataacaatct
    26701 gcaaaagcgg ttgtctcagc agactcttca ttacggatgg cttctctctc tcacgttgtt
    26761 ggaagtaggg tatgaccatc ccccgtttat tacaccaccg ctgaagaagt tagttcctgc
    26821 gccgacctgg ccccagcttg atagtgagta atgaactcct ggggtgtcac atccccaatc
    26881 gcgctgtgcg tccgctcttc gttgtattcc cgcctccagg cttcaatcac gagctgcgct
    26941 tcctgcaacg tcagaaacca gtgctcgttc aagcactcgt cccggaactt gccgttgaag
    27001 ctctcgataa acgcattctg cacgggcttc cccggttgaa taaagtgcaa gtggacgccg
    27061 tgctgagcgg cccaaacatc caaggccgtc ccggcgaatt caggaccgta gtataagcgg
    27121 aagcgaggcc atacgcagtt catctctcct ctagaatgca ggtacccact cactctagca
    27181 ggagggaccg cctgccgagc cctcgacagg agttcttttc gtggccatcg acgtggcgaa
    27241 gcaaagccat gaggtgctga ttgaaccccc ggccggaggc cgtcagcggt ggcgcatggt
    27301 gaactcgtaa actcccccgt caaggagaca gtgtcatggg agaatgtctg gcttctgacc
    27361 ttagccccgt tctgagacgg ttcttaacca gagcagacga cttcttcgat ggtcggttgt
    27421 ccctgacgtt gtgcgacgaa ctcgttcggt gtcaggtgcc cgagcgagct gtgtgggcgg
    27481 tggtgattgt aatccatccg ccaggcttcg atgatagctt gcgcctcggc gagcgacgca
    27541 aactggtgca cgttcaaaca ctcgtcccgc aggcgcccat taaatgattc aatgaaggca
    27601 ttttccaccg gtttgcccgg tcgtatgaag tcgagttgca cgccccgacg ataggcccaa
    27661 tcctccaagg cgcgtgattg gaattcggtg ccatggtcga cggtgatgga ccgagggctc
    27721 cggccttgcc tcaggacacg atcaagcacc tgactcaccg tctctcccgt cattcgaaat
    27781 cccgcctcta ggaccggact acaacgactc cagttatcta tgaccgtcaa gatccgaaat
    27841 ggccgcccgt cggccagggt atcatggacg aaatccatgc tccagcgttc ggtcggcccg
    27901 acagggatcg gggcgggtcc gcgatgcaat gccgtatgct tccgtctccg cacccgcatg
    27961 cgcagctgca atccatcgag gcggtagagt cgatggacac gtttgcgatt caccggccag
    28021 ccttcgcggc gcagtaacac ccagatgcgc atgtagccaa accgaggtcg ggcatgtgcc
    28081 agatcgcgaa tgcgtagtcg taaggcagac tgatccttgg cccgactcag tcgataccag
    28141 gaggctcgcc caaactgcgc caaccgacag gcccgcgcac aactcaccga gaacgtatct
    28201 tgaaaccagt gagccaattc tcggcgccgg gcgggcctca gacttttttt cgtagggcct
    28261 ccgagagcat gtgcttgtcg agggagagat cggccaccag ccgtttgagc cgggcgttct
    28321 cctcttccac ttgccgcagc cgacgcagtt cgctcacacc gaggtgcgcg tacttctttt
    28381 tccaggcgta gaaggtttgc tcagcgatcc cgagctgtcg acagacatcc tcaactgggg
    28441 tcccactctc cgcctgccga atggcataga cgatttgctc ttccgagaac tttgaccgct
    28501 tcatcgatcc actcctttcg tcgaggaccg ccgaagccgt cattctactc tcgttttaga
    28561 ttgccgtcgt tttcagggga gacgtcaagc tcagccaacc agatcgcctt ggtgctttca
    28621 gctcctccac tcgcgccgtc actcaatgtg agtgaggact tcgtactcac aactctttga
    28681 acttcgttcc agcgcctcct tcaggatccc ttccccttct tttgagtccc taccactggg
    28741 gtgattgtcg cccgggcctc ctcaaggtac acatgacagg gtcttgctga cactgattcc
    28801 ggccatgact gttgccgagt gggactgctg gattgccgga ctttcgtgga gcacaggacg
    28861 actttcttgg agacctgctg tatgcatgaa cctgctttgc gaccatggtc tggcatcacg
    28921 gctctggtcc ttgtggccac gttgttgacc agttgtgcct caccgacctt acgacgcgct
    28981 gagcgtttgg ccgaggacgg tgaatgggat aaggctgtgg cggcctaccg agaaacgctc
    29041 aagaacgatc ccttcgatga agcactcaaa gacaagctcg aagtcgccaa gcagcatgcg
    29101 gcggtggtcc actacgccaa tggccgccgg tcgcttgagg agcgtcgaat cgcggaggcc
    29161 ttgagcgaat tcaaggcggc gctggggctc gatcccacga aggtcgagca ccatgcgggc
    29221 atgaccgatg cgctgcggct gaaggaagct aacgatcagc tgcaaacggc cgataagctg
    29281 ctcaacttgg gacgatccga ggaggccctc acggcctatg aacgggccgt cgaactcgac
    29341 ccgagtttga cacaggccct cacccatatc actgagatca cggcccagca gcgggccacc
    29401 aagatgttcg gcggatccag ccagcctatt tcgctgcgct ttcaaaacgc caagctcaaa
    29461 gaagtgttcg agatcgtcgc gcggacggcc gggctgaacg tgattttcga caaggaagtg
    29521 cgcgacgaac ccatcacgat cttcatcaag gatatggctt tcgacgatgc gctgaacctc
    29581 atcctgaaca ccaatggcat ggttgcccag cgcgtggccc cggagacgct gctgatcatg
    29641 ccgaacaata aacagaagca ggcgcagtac caggatctct tgatgcgaac gtactatctt
    29701 aataatgcga aggccaaaga cgcggtgaac ttgctccgga ccatgttgga gagcaagcgc
    29761 atctatgtcg acgagaaggt caatgcgatc gtagtgcgtg atgaaccggt gaagctgcaa
    29821 cttgccgagc ggttgctctt tgccatcgat cgtcgtgagc cggaggtgga gctggatgtc
    29881 gaggtgctgg aggtcaatcg caccaagagt ctgaagtatg ggctgaacta cgccaaacaa
    29941 gccggcagcg ggctggttcc cacgggtggt tcgggcggga tttccacagc aacgacgcag
    30001 ttcacctatc aacaactgac ttccattggt ccgcagagtt atctctttac gctgcctgcg
    30061 agtgtcattt tggatttttt caaacaagaa tccgacgccc agaccctggc ttctcccaaa
    30121 ctccgtgtga tgaacaacaa gtctgcgtcc atcaatgttg gggataagca gccgatcttg
    30181 ctctcgacga ccaacgtgtt gccgggccaa gccgtgaccg gtgcggtgcc gacgacgtcg
    30241 accgtgacct ccatcgagtt caaggacgtc ggcgtgaaac tgacagtcga accgaccatc
    30301 aatttgatga acgaagtgat gatgaagatc aaggtggagg tgacgcggtt aggtgatcaa
    30361 gtcacgctgc aagcggcgcc ggagatcaag cagttcaaat ttgggacgcg taccgcagag
    30421 acggtgttga tgttgaaaga tgacgaaacg gtgatcttgg cgggcctcat ccaggacgaa
    30481 aaacgcaaga ccaagtccac gattcccctc attggagaca tccccattct cggagacatt
    30541 ctgacctcca agcaagatga tcgggtgtcc acagaggtgg tgttgacgat cacccctcgc
    30601 gtcgtgcgga acatggagct cccgtcctcg gccgcacaag ccttttggtc tgggaccgag
    30661 aataccttcg ccacgtctca gctctatgcc ccgcaagcga ttcctgtctc acaatcatca
    30721 ggccagagtc agcggtctca cactcctccc gcgccattgg ggtcggtgca agggactcct
    30781 tcagtctctc ctcttcacac gacgccgaca cacgacacga tgttggctcc aggcacaaaa
    30841 tcaccacagg ggattgctcc gacatccgat gtgtcctcgc tcccgagcgg tcagggacaa
    30901 gctcttgcca ctccagtccc tgccgctgag ttggtcgctc gatctacggg gtcattcatc
    30961 cttaagccat tggatgttgc taccgccgta ggacaggagt ttcgcgtcga tctcacggcg
    31021 ttgcatttgg aacaggtcag tgagacttcg gtgatggtga cgtttgatcc caagatgttg
    31081 gagtttcgtc gagtgacgcc tggatcggtc gcgctggcat ctcgaacggc tgatggccag
    31141 gtcatgttga cgcttcgccg ggacggtaca gtgccgccag gcgaagggct ccttgccatg
    31201 ttgttcttcc atgcaaaatc gccggggcag tttcctgtgg acctgcaggt cttgcctggg
    31261 aaggcatcta cgatggggac tgatgtcact ggcaccagcc gcgccatcat tcatgcgcga
    31321 tgaggttgct ggtgaaaccg acagtaaaac tgacgaatcg aggttttacc ctcgtcgaac
    31381 tcatgatggt ggtgaccatt gtggggattc tggcgaccct ggctattccc tcatttcagc
    31441 gctcggtcat caaggcgcgt gaggggactt taaagtatga cctggccacc ttgcgtgacg
    31501 tgctcgacca gtatcgtgcc gacaagggca cctatccatc agcgctgggc gagttagtgc
    31561 aggtgggcta tctcaaaaga attcccgtcg atccgtttac tcactctgac caaacctggc
    31621 aggtgatggc cgacgaaggg gagagtggcg ggattgccga tatccacagt ggcagtccgc
    31681 tcgtcggacc agagggcact ccctataaca catggtaggt tgatgcgtcg tatttcccgg
    31741 agcctcacag gtgccgtcac tctgatcgcc ggtggtgcga tgctgattgg ttgcgcgggc
    31801 cagcgtcagg agcgactcgt ttcaaatgat acacgggagc aggtcatggc tgttgcacgg
    31861 gagcgcgagg cggaactctc gacgctacgg gttgaaatgg ccgcgacccg gattgccgca
    31921 gcgaagaaag aggccgaatt ggttgagctg cgggccctgg tcgctcaact ccgacaggag
    31981 aatgcggata gtcaccagcg gctacttgag ttgcggcagg caggtgaggt tcgccaggcc
    32041 gaggtgaatg ccctcaaggc agagcgggac caattgttgg tcacgaagaa tgaccagtcg
    32101 ctcaaagacc ttaaagatgg cttgatggtg ctcaccaaac aactcgaaga cgtacggcag
    32161 gacatcggcc acaagacctt gcgtgcttcg gataagactt ctcccgcgcc gatgggaaaa
    32221 acttctgcac catcgcgacc agcgatgact ccatcgtcgt tgcaagggac tccagctcaa
    32281 ttctatgacg gtccggcttc catatcagcc atctcggtgt tggccgtcaa tgccacccag
    32341 tctccatcgg atgtgcttcg ggttcgtgtg cagccaggag attccttgtc aggtctggcg
    32401 cgccgctacc gcaccacggt gacggccatt gcgcaactca atcacttacc tacagatgtc
    32461 attgccgttg gccagggatt gctcgtgccg tccccaagct cttcacctag tgaccgggag
    32521 cgttagacga tgccgacact tgttcgacaa gcgacccttc ttgatgtctt ggttcgacga
    32581 ggttgtctga cagcagctca ggctgaagag gcgtcagctc aggtgtcagg tcccgaacgg
    32641 caactgtacg tgtcgttggt gggaggtggt ctgatttcgg acgtgcaatt ggccgaggcg
    32701 ctggccgagc agtgtggggt gtcctaccac tcgcttcacg agttccgggt ggactttgag
    32761 gcattctcat gcattcccat cgatattatg cggcaatttg aatgcgtgcc ggtgggagag
    32821 gaagacggag aactcctact ggcgctcgct gatcctaccg atgtgcggcg actcgatcac
    32881 atcgaacggg cggttgggag aagctgtcgg atcctcgtcg ccccgcggtc tgccatcgcc
    32941 gagtcgcttc gtgaaagcac gcgaaatagc caagtggtgg cgaagatcaa agatgagttc
    33001 cgtccgcttc tggtgcatga aaacgatgaa ggcgaagaag tcctctccgt ggacaaggtt
    33061 cagcgggacc tgagtcctat tgtccaactc gtcgatacga tcatcctgaa cgcgctgcag
    33121 aaacgagcca gtgatattca cctggaaccg gccgatggcg tcacggagat caagtatcgc
    33181 atcgacggcg tcttgtacct ggccatggag ccattggatc tgcgattcca caatccactc
    33241 gtctcgcgca tcaaagtcat gtcggatctc gatattgcgg agcgtcgggt cccgcaagat
    33301 gggcggttta agctccgctt ggataaacgc accatcgatt ttcgtatctc cgtgattccg
    33361 agcgccttcg gggaatcggt cgtcattcgt attctcgcca aggaagaatt cgcggcgaag
    33421 cggaacggcc tgcacctcga ttcgttggga ctgaagaaag aagatttaga tcggttccgc
    33481 cgcgccatca cggcttccta tggcatggtg ctggtcaccg gcccgaccgg aagcgggaag
    33541 acgacgacgc tctatgccgc gttgaacgaa gtgaacacca aggaagacaa gatcatcaca
    33601 attgaagacc ccgtcgaata tcagctggcg ggcgtggtgc aaatcccggt gaacgagaag
    33661 aagggcctga cgttcgcgaa ggggttgcga tcgattctgc gtcatgaccc agacaagatc
    33721 atggtgggtg agattcgcga tgccgaaacg gcacagatcg ccattcagtc ggccttgacc
    33781 ggtcacttgg tgttttccac ggtacacgct aacaatgcat ttgatgtgat cggtcggttc
    33841 gtaaatatgg ggatcgagcc ctataacttt gtctcttcgt tgaactgcat catggcgcag
    33901 cgcctggtcc gcaccatttg tgagtcgtgc aaagcccctc tggctgtttc gcagacccag
    33961 tgtgagcaac tggggtttga ttacgcgctc ttcaaggatc gtcctgtgta tgagggaaaa
    34021 gggtgtttgg actgtcatgg attgggttat cgcggccgcc atgccatcac ggagtttctg
    34081 cccctgacgg atcgaatcag ggaactgatg ttagatcggc gcccgccttc tgagatcaga
    34141 cgagcggcgg tcgaacaggg gatgaccgat ttgcgtcaag ccggcgtgga aaaggtgcgt
    34201 cgaggtgaga ccacgatgcg tgaagtcaat cggatgacgt tcttggggta gtgatatgag
    34261 agccacactc tttcccatcc atcgtccccg tgtcggcatc agcgtgtcct cgtccagcct
    34321 cagtgtggtc gtgcttcgac ggccctggtt ccgcatgccg atcgttgaac aggtgctgga
    34381 gcgtcccttg cccggtggct tgctgaaggt ttccacaacg gagccacaca tgaccgatgt
    34441 ggacgccttc gcgaaggaac tgcgcgagct gacggagggt ctgagcgagc gaacgattgc
    34501 cctgagtctg cccaatcgag cgatgcacat gggggtgttt gcattcgacc gttttcctca
    34561 cgacccagag gaacgtgaag gcttgatcaa gtggcggttc cgcgaggacc tcaacctgac
    34621 gattggcgac gggcgggtga tgatgcagat cttcccagcc ggaagaggca cacaggtgct
    34681 ggccatggct gtgcgccaga cgatcttgga tcaatatgag gcagtctgtt cgcgggctgg
    34741 gctgatcccc gcttcaatag gattctcgta cattcagctg atcggtctct atcgtcgtct
    34801 catgaagggg catcgacggg tgtgcgtgat gcaatgggcg gacgacgaat tcgtgctgtt
    34861 gctgttcgat caggggaaac cagtgtttct ccgcacaaga tcgatgaccg gtaggacgca
    34921 tgatcttcgg cgcgagctgg tcggcacgct gcaatacatg gctgatcagg ctacgactgc
    34981 tcatgaagcg ggtgagtcgt cgtttccgct gtacctgatc agcagtcagt cgaaagaatc
    35041 gatcacgcag ctgctcggag gggaggcgac ggtgaaggtg cctggccctc agggtggatg
    35101 gcagatcgag atcgtgccgc tgggatggga cagtgttcca gctcatctca acaaccagac
    35161 gggatcgttg aacggactct cggcgatggc gacggtggtc ggattatgag agatctggtt
    35221 ggcctggcgt ggtctccgtt cctgcgctcg cagagtgcga ccaatcccgt acctcacttc
    35281 gccctctcgg tcacgccggc tgccattacc atcctgcgtg tgatgcagtg gatggccgtc
    35341 gtcgtgttct gtgcctcatt ggcagttgcg ttcaagtggt ggaacgacag tggggtgatg
    35401 gaggagaccg ccacacatct cgatggggtg gtgcagcggt tgaatcacgc gaatgctagc
    35461 cgttcagagg ggatgacccg cgagcgactg acgttgtccg tggatcagat cgcgaccatc
    35521 aagcgcgatg tgcggttcgt caatcagctc gccgagaagc ggacgttctc atggtcccgt
    35581 ttgttggcgg atcttgagac caccatcccg ccgcatatgg cccttcgcac cgttcagctg
    35641 aactttcagg actcaaccgt ggcgcttcat ggatcggcgg cttcgctgca agacatccat
    35701 acgttcatga ccaatttgga acaccatgcg gcgtttcggc atgcggcgtt ggccagtcat
    35761 cgccttgagc atcaggagag tcagccgtcg caggcgagca tggctacgcc ggacacaatg
    35821 caacctgcgc gaggcgtaga ctttcagttg accgtaggct atcgaccgtc gttgtaggtt
    35881 gaaagcatgg cgaatctgct cagacatata cagttgccag tatttagccg gtcgcttctc
    35941 atgtcctgcg tgccgttcat gagtgttacg gtattcgcct gtatccttgc tgcggcaatc
    36001 tactgctaca gactgagccc aacgcaggaa catctcgctc aggccgaagc ggcctatcaa
    36061 accgccttgc gagctcaggc agcacttcag gctaatcgca agacacagga agagatgcga
    36121 gcgatcaagc tgcagttgga cgaggtctgg cggggccttc cgaccgagac agaatttgcc
    36181 tcattggcgc tcgctatttc tgaactcggg cggagcgagc gagtgaacat tcctgggatg
    36241 cagtatgggg tcgagcaatc gaaagccagt ggaatgccgg tcaaggcgtc gatctcgttt
    36301 tccgtgaccg gcgactatgc ggcggtctat cgtttcatcc accgattgga atcggctgat
    36361 tcctatgtgg tcatcgagag tctcgacgcg agtcgggctg cgaaaagtga caagggggtc
    36421 tcatcctcgg tcctgtttca tgtgaaggtc gtgacctttc tgcgaccaaa tccaccgagt
    36481 gggagtatgt catgaagatt cggaccaaga ctctgatact tggcgggctc gtggggctct
    36541 gggccatggt cgctgtcgta cagatcggca gtttgccaga gtctcaggag gccccactga
    36601 cctacgtgtc ggggcagcgt gtcgcgaggg gtgtgaagtc aaccggtact gatggtgagg
    36661 ttatgctacg tcccatgcgg gtgggggtgg cgcaactccc ggccaccccg aagcgaaata
    36721 tcttcgaacc ggcagaaaca ccaccttctg aaaagcgcct cgtcagggca gaggccagca
    36781 agaaagaggt gacaccgccg atgcccgtga cggtcgttgc catgccggtt gttccacccg
    36841 cgccaccgat gccttcgccg gaggaattgg ccgaacaggc agcgcgccaa gcgcaggaac
    36901 gcaagcgtca ccaacttcgt gagttgatgg cgcagtaccg ctatcttgga tacctgaccc
    36961 gagaagggga gcagaaggcc tttttaggca aaggccagga gatctatatc attcatgccg
    37021 gagacaccct cgacggtcag tttcaggttg cctccattac tcccacggca gtcaagatcc
    37081 tcgccgccca atccgacgtc gaaacgacga tccagctgaa gaccgagtca tcgatagaac
    37141 catcataatt catcgctctc gttcactctt gcgagcgata tcccaccgct cggtcctgcg
    37201 tagtcgcacc tgttctcagg cgcaccatat caccaatagg gccgaacagg cctagcatac
    37261 ggcctcttgt ctcgatccat tttagtcatt caaaaaacac gaaactgttc gccacggaga
    37321 agatgacgtg gtagtgaagg ctacgtacgc cagttcctcg cgtgcatcac gcaccattcc
    37381 ctgctgttca ccgctcacga aggatcgtcg atgacccacg cgaccgtttg caccggcatg
    37441 ttacgtcgat ggatgaaggt ggtggttctg gctggcttgg ctggaatcct cggtccgttc
    37501 tcctcggcct tggcgcaatc ctcgacggtc actctggatg cctatctcca tgggagcggt
    37561 gggaccgcca atccaccgac cctcacgtcg gatctgattc caccgctcag cctcacggcc
    37621 aagaccaagg attcagccgg cctcgcattc agtggcggca atccctgggt gacgatgggg
    37681 tcctggaccc cgccggccgg gatcaacggt aaagtgacac tcacagcgct caccactgcg
    37741 cgtctgtgga tgggcttgaa aagtagcggc gatgtcgggg gcaaggtcga tctgttggtc
    37801 gaggtgctgg tgaatggcca agtcctgagc tcagggttga ctcgctgcct gagtaccctg
    37861 accgcgtctg ccagtaccgc ccaagaaatc agcatcgcgc tgggcagcat cccgacgacc
    37921 cagcttgata cgttgaccca aaccctcgcg gttcgggtct ccacccgcat ggggaccacg
    37981 gcgagtaata cggcttgtgg caccaaagcc agcgtgacgg gggtccgcgc ctatttcgat
    38041 gcgctcactc ggaccagccg catcggcgtc acgatcgccc tgccaccgcc gcaaccggtc
    38101 atcctgcttc cgaatccgaa aacgatccag gccggatcga ccagtacact gctggccctg
    38161 ttgttcccgg tcccgctcca ggcgacgacc ctgtcggtcg tcagcaccgc gaccagcgtg
    38221 gccactgtcc caagtagcgt cgccatcggc atcggacaag cctttgtctc catcccggtc
    38281 accggggtca gcgccggcac cacccagatc aaagtcaccc tcaacaataa gaccatcacc
    38341 ggcaccgttc gggtggtggg cggcgcggcc accatcagta gcctgacccc ctcgaccctc
    38401 agcatcaccc aaggcgggag cgggcagttg accgtgtcac tcaatgccac ccaggcgacg
    38461 aatacggtgg tcgctctcag tagttcggcc gacagtatcg cggcagtgcc ggctaccgtc
    38521 acggtgccgg ccggtcaggt gagtgccgcc ttcaacgtgg cggcgaacac ggcgggacaa
    38581 gccgacatca caggcaccct caatgggacc agtgcgacca gccatgtcac ggtcacgccg
    38641 gcattgcccg aggtggtctc gctcacgcct ccggcgagcc agctcacgct cggggccacg
    38701 acgtcattaa cagtgacgat ctctgcggcc caagttggcc cgacggtggt gaccctgagc
    38761 agcaccccca cgggtctcgt gacggtcccg ccctcggtga tcgttccggc tggccagacg
    38821 accgcctcgt tcaccgtcac gtccgtggcc ttggggacca cgatggtccg tgccaccctc
    38881 ggcagttcac tggccgaggc cgccatcgat gtcgtgccgc ccgtggtggc tctggtggat
    38941 ctgcaaccgg cgagtcaatc gctggtggtc ggggccatcg ggaccctgac cgtcaccttg
    39001 aatgcggccc aaagcagccc gacggatatc gcgctgtccg tggaccagcc gaccgtactc
    39061 cagattcccg cgacgcaaag cgtgaccgta ccgcccaacc agacacaggc cacctttacg
    39121 gtcacggccc tggccaccgg cagcgcgcag ctgacggcaa ccttaggcac catcagcaag
    39181 caggcgaccg tgacggtgac gccgcagcct ccgcaagtgc tctcgctcac gccgagtccg
    39241 ctggccgtgg tgcagggcgc gaccgggacg ctcacagtaa ccctcaacgc cgcgcaagcg
    39301 accgatacgg tcgtacccat cacgagcaac aacgccacca tagtgggagt cccggtcagt
    39361 gtcacggtgc cggccggtct cacgacggcg ccggtctcgg tgagtggcct caccattggg
    39421 gcggcgaccg tcacggctac gttgaatggt acggcgacgg cgaccgtgaa tattgttccg
    39481 cctccgccgg tcgtgacagc gctcgggccg attccgcccg tcacggctcc gttgaccttg
    39541 gccaagggcc gcacgggcgt cttgcaagtc acgatcaatc gcgccccgac ggatccgacc
    39601 gtcatcacgc tgcagaacag tgcgacctcg gtcgtgaccg tgccgcccag cgtcaccgtc
    39661 gctgccgggc agttgacggc ctcgttccct gtcacgaccc agacggaagg ccaggccacg
    39721 atcacggcct cgttcaacag cactagcgcc accgcacagg tcgtcgtcac ggcgccggaa
    39781 gtggaatcca tcgcactcgc tcctgccgcc ccgacggtct ttgccgggga atcggtctcg
    39841 ttcacggcca ccggcctgtt caccgacggc agcagccagc cgcttgcgaa tgacgtgacc
    39901 tggagttcga ccaatcagtc ggtggcctcc gtcagcagca gcggcttggt cacggcgctc
    39961 accgtgggca ccaccacgat caaggccacc gtcaccacgt ctgtcggccc ggtgacaggg
    40021 gagaccaccc tcacggtgca acccgcgcca gcattggtgt tgacgccact gacgacttcg
    40081 ttgacggtcg gccagagtgc gctcttcacg gtcgggacgg ccgtggcccc cacctcggcc
    40141 ctcacggtca cgctggccct ttccggcagc ggcaccgcgt tgttaaaccc cgtctcggtc
    40201 gtgatctcgg ccggacaaac gacctcgccc acgccggtac aggtcaccgc gaccgggatc
    40261 ggctcggtga tcctcacggc cacggcccca gtgcggcaac ccgcgagtag caccctgacg
    40321 atcaccgccg gcctccccgt gatcaccagc gtaagtcccg cgactggtgt ggtagggacg
    40381 gtcgtgaccc ttggcgggtc cagcttcaac acggtggcag ccagtaatca ggtgcgtttc
    40441 ggaggcatcg tggccacggt gctggccgtc aacaccacag ggacttcgct caccaccgcg
    40501 gtgcctcccg gagccctcac agggccggtc acggtcacga cgccgctcgg cacggcgacc
    40561 agtccactgc ccttcacctt gctcaatagt ccgccagtca tctcctcgat cgggaacaaa
    40621 acgctcacgt taggcagcac gctcgcgttc accgtcgtcg gggtcgatcc caacggacag
    40681 ccagtgacct atggtgcgca gcaggccgcg ccggcgtcgc cgacgttgcc gtcgcatatg
    40741 gcgttcaaca gtcagaccgg ggtcttcagc tttacccccg acgcgacgca ggtgggcacg
    40801 ttcaatatca cgttcctcgc gtccgatggt gtggtgtcct catccgaaac cgtggccatc
    40861 accgtgaccg ggacggtgcc cggcgcgcag accagtctct cgggccgtgt ggatgacagt
    40921 tccggcaatc ctgtggccgg ggtgaccgtc tcgctcaagg atagtccaac cacgacgacc
    40981 accaacgcga gcgggcagtt caccttgacc tttgccggca cccccgccgg acggcaactg
    41041 ctggtcgtga ccggggtccc ttcgggctat gccaatctcg tggccccggt ggatctgatt
    41101 cccaatacgg cgaacgtact cccgagcccc gtgaccacgc cgccgttaga cctggccacg
    41161 gcggtcaccg tcaatccggc cgcgacgacg accctcacca gcgccacggc ccatgtcacg
    41221 gtgactattc cgccgggccg ggcgaagaat gccgacggca ccgcctttac cggccaactc
    41281 accatcagtc cggtcccgga atatggccgg ccggaatctc ggccggtgga attgcggcct
    41341 ggtctgtccg tcacgattca accggcgggg gtcatcctcg acccgcccgt ccccatcacc
    41401 tttccgaaca tcgacaatat gccgccgggc aatgaactcg acctctggtc gctctatccg
    41461 gacaccggta ccttcctcgt tgttggccgt atgcgggtca gcgccgacgg ccaacgcctg
    41521 gagaccatcg acggcggcgt gcggaagaca gcttggcact atgccctccc tccgaaccct
    41581 gtcccgaacc aagtgccgac tcagcaagcc gggccctgta cgcaatgtgc attcgcctcg
    41641 cagggagatc tcaccgaggg ggccttgacg gagacggtcg cgattcccgg catccgcagc
    41701 ctcggagtct cacggaacct cactctgcgc tatacctcca ccacggcgga tgtgcagccg
    41761 atcctgccgg tgaatgcgtt actgagcgta cgggcggccg tgccgccgac cttctccgcg
    41821 cggctcagcg tcggcggggt gcaacaggga caagaagtct attgggatag ccatgctctg
    41881 ccggagaatg ataattccac ttcgcggctt ggcctgcaga tggatgcgtc aaacctctcc
    41941 accggctcct atccgtatga actgatgctg ttctccaatt acccgcagtc gagcattggt
    42001 ggggcgagta tcgctcgcat gttggtccgc aatgaacaac agaatcccct gggggcaggc
    42061 tggactctcg acggactgga tcgcctctat ccgcaaccgg acggttcggc tgtcttggcc
    42121 cagggcagcg gtagcacgca gtggtatcgc ccagtagagc ggcctctcgt catcgagagt
    42181 ttcgatcaag cccgcagcct gacggtcggc ggcgtgaatg ccagcttcgt gtcgggcgcg
    42241 atccataccg gagccaggaa tgatctcctg aatcccgcca actttggggc gcagggcaag
    42301 gtgcggcgcc ctgtcacgct acgaagtggg atcaacaccc tcacgctgtc ggctctcacg
    42361 ggcgtggatc tgtttgtcct caatctgctc gccacggaaa tcaccgcaac cgaggcgcag
    42421 gtgttggaac aatttgtgcg cgagggcggg acggtgctcg agatacggaa tgcagccccg
    42481 gcccgccccc tgctgtgggg caccgtttcg ggtccgtttg tcggggactc cacggcggat
    42541 ctgaccctcg acggcctcaa tgcgatggtg agcaacgggc cgttcggcac ggtcgtgagc
    42601 ccgctcctga ccggcggcaa cgccggctat gcgaccacgg gctcggccac ggtgctggcg
    42661 cggaacgacg tggggcccaa cgtgctcctg cttccgccca gcacggtctt ctccggcgcg
    42721 ggacgcgcgg tcttggtggg tgatgaagag atcgtggcct cgggctttcc cagcggcgcc
    42781 ggctcgaatc ggtacgccga ggggtcgaat cgcacattct tcctcaacac cattgcctat
    42841 ttgtccgacg ccccgggcta taaccccgtc cttcctccga acaccaccgt cgtaacccta
    42901 caaggcccgc ccggtgactt ttcggtgctg gcgcggcgca ccgatagcac ctatacgagg
    42961 atcctgaaag acggcacgac ctatcagttc acggcccagg gcctgcagac cagtgtcagc
    43021 gaccgcaacg gcaatcagac gacctatgcc tacgacggca ccgggcagct tctgaccatc
    43081 accgatcctg tcggacaggt caccaccttc gcctatagcg gcagccgact cgccaccatc
    43141 agcgaccctc aagggcgggt cacccagttc acgcacgatg gtgggggcaa tctcaccggc
    43201 gtgacctacg ccgacacgag cacggccacc ttcgcctatg acccgcaaca tcgcctggtc
    43261 aagaagaccg atgcgcagaa ccaggtcacg cagtacgcct atgactatgc cggacgcctg
    43321 agtcaggcca ccttgccggg cggcgatcgt cggagcctca cccccagcca acggatggcg
    43381 gtcccgaatc ttgcggcagg ccaggggacc gcgacgacac cggcggcctt aagtccgagc
    43441 acgatcaacc aagccggctt caccgacgcc aagaaccaga ccacgcggtt tgagaccgat
    43501 gcactgggcc gcatcaccaa acagaccgac gccttgaacc gcgtcaccac catcgcccgg
    43561 gatgtcaacg gcaatcctac gcagatcgcg cgcccgaatg gcgccatcac caccatgacc
    43621 tacgacgcca agggcaatct gctcaccagc accgagcagg ctacagctgg cacgaccacg
    43681 ttcacctatg agccgacctt caatcaggtc accagcatcc gcgatccgaa ggggaacctg
    43741 acccagatcg cctacgatgc gaagggcaat ccgctcacga tcacggacgc cctgaaccag
    43801 gtgaccacgt tcacgtacaa tcctcagggt ctcttgctca cgaccaagga cgcgctgaat
    43861 cagaccacca cgtttaccta tgatgccttg gggcgattac tcaccacgac cgatcccctg
    43921 acccgcacca ccaccctgac ctatgaccaa gcgggcaatg tggcgacctc caaagatgcg
    43981 ctcaaccgca cgaccagctt tgcctacgat cccaagaacc ggctcactca ggtgaccgat
    44041 cccaccggcg gcgtgacgcg ctatgcctac gacgggaacg gcaatctcct gaccgtcacc
    44101 gatgccaaga atcaagtgac cacctttgcg tacgacagcc ggaaccggct gctcagcacc
    44161 accgatccgc tgggcaagat cgaaacctac acctacgacg gcaacgacaa tcttattaag
    44221 cggcggactc cgaagggcga cgacatcctg ttcgcctacg atgccgtgaa ccaattgctc
    44281 agcaagaccc tgccgagcag ccaggtcacc agctacctct acgacctggt cggcaacttg
    44341 accaacgtga ccgatcccga tagcgtactg gcgatgagtt acgatttagc gaaccgcctc
    44401 acgaccgtca agaccgatgg ctcgccgaat caaccggcgg tgaccttgag ctacacgtat
    44461 gataagaacg ggaatcgagg gagtgtgacc gatccagccc agagcgtggc gtaccgatac
    44521 gacgggctga atcggctctc gcaactcggg gtgccggggc cggccagctg cccaactttg
    44581 ccgtccgggc tggtgagtcg gtggctggct gacggcacca cagcggatgc ggtcgggagc
    44641 aacccggcga cgctccgcaa tggcaccacg tttggcctag gcctgttcaa tcaggcgttt
    44701 cacttcgacg gcgtcgacga ttttgtggag gtgccccatg cggcctccct catcccgccg
    44761 gcgctctccc tgagtttctg gctcaaactg gacacgctga cctccaccag tgtctcggag
    44821 caaggcctgc agtgggtgtt cttcaaaaag aacagtcgcc cgtccacgac cctcttcaat
    44881 gactacggct tcgccaaggt ctttcgagag ggccgccact atgtggtgtt tggcatcaat
    44941 gccgccagcg gtcagcccgt ctttgtgaat tcgacgacga gcgtggtggc gggcgtctgg
    45001 taccacgtga tggccacggt cgatgcgcag gagttaaagt tgtatatcga cggagccctt
    45061 gagggcacga gtcccgtcgg attcccgctg gatcacagca ccagtccgtt gatcttcggc
    45121 gctaccaacc agagcatcta tgacggcaag ctggccggca gtcttgatga catccagttc
    45181 ttcaatcgcg tactctcccc ggccgaagtc ccccagacga tgtgtcagga cagcgcgcca
    45241 ttggggcaag ccacgaccta cacctacgat gcgctctcgc gccggacgag cctgaccctc
    45301 ccgaacggcg tgcagacgac gtatcagtac gatcccgcga gtcaggtgac cgcgattagc
    45361 catcagctga cagccatcag ccagcagatc aataaagccg aatacggcta caacgcggtg
    45421 gggaatcgga ccagtctcac ggacagaagg ggagtccaag ccttcggcta tgatcaattg
    45481 gaccggctga cgagtgccag ccaccccttg ctgctggacc cgcaagcgtt cgcctatgat
    45541 gctgtgggca acagaaccac aggcggcagc gtggtgaatt caggcaacca acttacgtcc
    45601 gatgccaact tcgcctatca atatgatgac aacggcaacc tgactcgcaa gacgctgctg
    45661 gccaccggca attacacgca gtacacctac gatgccgaga accggctgac gcaggtgcag
    45721 gaattcgcgg ccggcaatcc cacggccgcc acgacgagca cgtatcggta tgacggcttg
    45781 ggtcggcgga tcgagaaggt cgccaacggc caaacgaaac ggtatgtcta tgacggcgcg
    45841 aatgtcctgc aggcacgcta cacgcatggt cacgggatcg atgaacccct gagtcgcacg
    45901 ccggtcacgc ccggggtcgg gagcggccat acggccgtgc tgacctcgac tcccgatggc
    45961 ctacgcggtc cggtcgtaga tgatgggatc agcgtgaacg ggcaggtgtc ggtgggcttt
    46021 accctggtct ccggccagcc cacgccggca gtcgggcaac cgatcgacag caccggctcg
    46081 caccagccga tcgcgccgat cgatgtcacc gccgcgacga acagcgggac gctcaccgtg
    46141 aatctgatcg ataccggcgg gatcggtggg aactcggctc tgtatctcgt cacgcgcgac
    46201 caggcgacgg gacaggccat tgagagccgc ctgctgtttc cggctcgtgt caccttcgcc
    46261 tcgacgacgc ccctgggcac cccgctcgtc gtagcctcga cgacggtgcc tacgaccacg
    46321 ccggtggccg acggcaccct gttctaccat caagatgggt tgggaacggt gacggagctc
    46381 accgatagca acggcagcgt cgccaaggct tatgcctacg atgcctacgg caatctcctc
    46441 gaatcacccg gcactgttga gcagccctat acctatacgg ggagggagtt cgatgccgag
    46501 acgggactct attactaccg agcgagatac tacgattcga tgacgggacg gttcttacag
    46561 aaggatccga ttggtctggg tggtggcgat gttaacttct acaattatgt tcgaaacaat
    46621 ccacctcggc tagcagatcc gtttggattg tatgatattg caactccagg tgaagtgctc
    46681 aatttctggg gcgagttctt tggagccatt agcgactttg gagcgaacta tgtcgatatg
    46741 cgacaagcct ggtggaaagg cgcagacaag tatttccatt gcaaggccaa ttgccaggcc
    46801 gctcagcgtg gcgtggaggg tgaggctgta gcgtgtatca tcagtgatat ccgtgaatgg
    46861 tgggatcaga atgtgaagtc ttactgggag ccacgatcca caccacaaga cagcgccgca
    46921 gatcagcaag caaatctgta cggtcgtata caagggagta ccaaccctgg agcactatgc
    46981 gaacagtcgt gcgctccatt tagacccgcg gggttgcctc cacaatattg agctttcagt
    47041 ggagaggcct ttccgaatgg ttagattcct gttcacgtta atggcgctgc ttctagttgg
    47101 ttgcgactgg attgaatcca aggaaggtca ctatccagat atgaaagcag caggcgcaac
    47161 aggggagatt tcaagagggt ggatacccgc atttgtgcca gaatctgcag tggatatcag
    47221 acttaagtat gatgttgaga acaatcgcac ttggctttct ttccgtggag tcatcgatgg
    47281 atccgcgctt tctggggcgt gccttagtgt gactacaaag aatgtcactt acccaggcaa
    47341 tggaccgcgc gagtggtggc cgggtgctct aactgttgac tcaaggggga gcaagacaga
    47401 atatgagtac tacgcttgta aagatggaga gatgttggca atagatcgga caaaagcaca
    47461 agtgttcttg tggagattag ccaagtgatg gtgtattcga tcgggcttcg gcgggtacct
    47521 tcaacgaaac cgcacagggc ttgcagtacg atgcggccag ccagctctca cccatcagct
    47581 aaccgccgtg agccaactga tcaacaaggc caactacgtc tacaacgccg tgagaaacag
    47641 aaccagcctc acagaccgca gaggaccgca agccttcggc tacgatcaat tggatcggct
    47701 caccagcacc agccatccct tgctgctgga tccgcaagcc tttgtctatg atcccgtggg
    47761 caatcggacg acaggcggtt ccgtcgtgaa tgccggcaat cagctcacgg cggatgcgaa
    47821 tttcgcatac caatatgatg acaacggcaa cctcacgaga aagacgctac tggccaccgg
    47881 caattacacg cagtacacct acgatgccga gaaccggctg acgcaggtgc aggaattcgc
    47941 ggccggcaat cccacggccg ccacgacgag cacgtatcgg tatgacggct tgggtcggcg
    48001 gatcgagaag gtcgccaacg gccaaacgaa acggtatgtc tatgacggcg cgaatgtcct
    48061 gcaggcacgc tacacgcatg gtcacgggat cgatgaaccc ctgagtcgca cgccggtcac
    48121 gcccggggtc gggagcggcc atacggccgt gctgacctcg actcccgatg gcctacgcgg
    48181 tccggtcgta gatgatggga tcagcgtgaa cgggcaggtg tcggtgggct ttaccctggt
    48241 ctccggccag cccacgccgg cagtcgggca accgatcgac agcaccggct cgcaccagcc
    48301 gatcgcgccg atcgatgtca ccgccgcgac gaacagcggg acgctcaccg tgaatctgat
    48361 cgataccggc gggatcggtg ggaactcggc tctgtatctc gtcacgcgcg accaggcgac
    48421 gggacaggcc attgagagcc gcctgctgtt tccggctcgc gtgaccttcg cctcgacaac
    48481 gcccctggcc tgcccaccgt gatcgcctct acggcggtcc cgatcagcac cccggtggcc
    48541 accagcacct tgttctacca tcaagacgga ttgggtacgg tcacggaact gaccgacagc
    48601 aacggcgcgg tcaccaaagc ctacgcgtac gatgcgtacg gcaccatcct ggaatcgccg
    48661 ggcacggtgg atcagccgta tacgtatacg gggagggagt tcgattcaga aagcgggctc
    48721 tactactacc gagcgcggta ctacgacgcg acgacgggaa gatttagcca gcgtgatcct
    48781 atcggtcgag ctgggggaat caattattat gaatacgctc ttggaaatcc ggttaggcat
    48841 tttgacccat atggactgaa atcagaaatt gtcatctggg ccccagtggg aatcggacaa
    48901 tcctcctttg gtcatgtatc aactgacata aatggaacga cctattcatt tgggcccaac
    48961 gggatgacga ctgaaccgac cagtgtctac agacaaagaa actcgtttcg agattcgcta
    49021 gggttggatt tgaatctcac tcctgaacaa gaaaacgcac ttatggactg cttagggcga
    49081 agccagccag actataattt cctcactaac aattgcggag gtcctgcgca aaaatgtctt
    49141 aactggttag gttatgacgt tggccaactc ttgttaccgt cacaaattag tcagagtttg
    49201 gtgggaatgg gattagttag gagtacaact ttctatccgg gagcgacttc aactccttct
    49261 caccccaagt agaaattaat catggtaaaa ccctcgttag c
//
LOCUS       DAORQN010000004        35899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_68_length_35899_cov_28.919568, whole genome shotgun sequence.
ACCESSION   DAORQN010000004 DAORQN010000000
VERSION     DAORQN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 35899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35899
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68_length_35899_cov_28.919568"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK614_00695"
     CDS             <1..225
                     /locus_tag="PK614_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRB80764.1"
                     /translation="VLLPFFKERRMMLVDFKLEFGVFHNRLILADEISPDTCRFWDQT
                     TKESMDKDRFRKDLGKIEEAYQEVLKRVCE"
     gene            233..460
                     /locus_tag="PK614_00700"
     CDS             233..460
                     /locus_tag="PK614_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80765.1"
                     /translation="MNRFLQLFLNYGLVAAILVWAATVALMAYHLKESPWRWAFVLMS
                     LAGLGTIGVIFWIRKYVQRVGKAQQEVGKAQ"
     gene            457..696
                     /gene="purS"
                     /locus_tag="PK614_00705"
     CDS             457..696
                     /gene="purS"
                     /locus_tag="PK614_00705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRB80766.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAGNVRVGKYMEV
                     DVNEADRAKADAQVKQMCEKLLANTVIEDYRYELG"
     gene            810..1520
                     /gene="purQ"
                     /locus_tag="PK614_00710"
     CDS             810..1520
                     /gene="purQ"
                     /locus_tag="PK614_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRB80767.1"
                     /translation="MVVFPGSNCDHDCQYIFKDVLGQYVEMIWHKETLLAGLDAIVLP
                     GGFSYGDYLRTGAIARFSPVMGAVKAFANKGGLVIGICNGFQILLEAGLLPGVMLRNT
                     SLNFICKDVYVKVENAATRFTNQCESGQVLKIPIAHADGNYYTDPVTLGGIKANAQVI
                     FRYCTGDGKVTPDANPNGSLDNIAGIMNADGNVLGMMPHPERSAEAILGNEDGRLIFQ
                     SMLSSFGKPELQSKLIGV"
     gene            1589..3835
                     /gene="purL"
                     /locus_tag="PK614_00715"
     CDS             1589..3835
                     /gene="purL"
                     /locus_tag="PK614_00715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRB80768.1"
                     /translation="MNALVITKAILEQHKLSDDEYKKILEILGREPNWTELGMFSAMW
                     SEHCSYKSSRIHLKKLPTTGPRVVQGPGENAGAVDIGDGLCAVFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIALLNSLRFGGLDNANTRHLLKGVVSGIAGYGNCMGV
                     PTVGGEIVFNDIYALNPLVNVFCLGIAKKDKIFLGKAAGVGNPVIYFGSKTGRDGIHG
                     ATMASDSFDEQAAQKRPTVQVGDPFTEKLLLEACLELMAGDCLVGIQDMGAAGLTSSA
                     CEMASREGTGVELELADVPRREPGMTPYEIMLSESQERMLMVAKAGREDEVIKICRKW
                     DLDVAVIGRVTDTGKVVLKENGQMVAEIPAKALADDGPRYDRPSAPPAYQEMLQALNH
                     DALPDVKDVNAALLALLDSPTIASKRWVYEQYDHMVRTNTMVRPGSDAAVLRVKGTNK
                     ALALSVDCNGRYCLLNPYEGAKIAIVECARNLACSGAEPIGVTDCLNFGNPQRPEVMW
                     QFVLAIEGLKDACEALNVPIVSGNVSLYNETNDLSIYPTPMIGMVGLIEEADRAVTQW
                     FKESGDVIILLGQTREDLGGTEYLKVLHHREQGSPPLLSLETEKAVQACTIRLIRDGL
                     VQSAHDCSDGGLAVALTECCISGSAQQLGAVVQLSLDGLRRDALLFGESQSRILLSVK
                     AELAERVLNAAWDAGVHAARIGTVGGARLVIQVDGDQRAAGCKIDLELTALYAHWGLA
                     IPRALGQD"
     gene            3848..5278
                     /gene="purF"
                     /locus_tag="PK614_00720"
     CDS             3848..5278
                     /gene="purF"
                     /locus_tag="PK614_00720"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRB80769.1"
                     /translation="MANELPLISPDKFHDECAVFGIYGHKEAANLAYLGLYALQHRGQ
                     EASGIVSNDGEQFYVEKGQGLVADIFSQQALARLPGTMAIGHNRYSTAGGAGLKNVQP
                     LSVNFAFGNLAVAHNGNLINATMLRSELEAYGAIFQSTSDTEVIIHLIAHSRADTLLD
                     RVIDSLTQVRGAFSVVLMTDQGIVAARDPHGFRPLCLGRFRDAWIVASESCAFDLLDA
                     EYVREIEPGELVVLDHQGVTSYKPFAQTKPAMCVFEYVYFARPDSRIFGGKAVYSIRK
                     AFGRQLAQESRVDADIVIPVPDSGVPAALGYSEGSGFPFETGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLEGKRVVVVDDSLVRGTTSRKIVKMLRHAGAKEVHMRISSP
                     PIVSPCFYGIDTPTKKELIASSHTTEEIRKYITADSLAYLSLDGMVKAAPGTPGQYCD
                     ACFTEQYPISFTRAEELQLGLFEAPR"
     gene            5346..5786
                     /locus_tag="PK614_00725"
     CDS             5346..5786
                     /locus_tag="PK614_00725"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HRB80770.1"
                     /translation="MSLVRKYRVIVATGVVVVGVRIASRLTSLRRLLGWLLCEISADP
                     DEHAMEDVAYYVDRWLALFPYNPKGNCFPRALALFFFARRAGLPVQFKCGVMKLNQQL
                     EGHAWLVLEGHEFLEPSSYWQSFTVTVTFPQSLVSSSTQSSRIS"
     gene            6159..6476
                     /locus_tag="PK614_00730"
     CDS             6159..6476
                     /locus_tag="PK614_00730"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRB80771.1"
                     /translation="MPATVHLWSEPQLELSEMFPRQDEQVHSTVLDGESVLLNLNSGR
                     YYTLNVVGSTIWDLCRGEQSLRQIHSAICARFDVSEQQAQDDMLALIVLLEQEGLLHT
                     ERR"
     gene            6718..7752
                     /locus_tag="PK614_00735"
     CDS             6718..7752
                     /locus_tag="PK614_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80772.1"
                     /translation="MASTMKELLLSVHGIEMKFTTNSSELAHSVWALLRHFHRDTVAA
                     QPMRMEFDAIERREDVPVQISSSAKLLYSGSGLAIDPDRKTTWLCDIYSDNGLLIADF
                     HGEGLVVIDGDSQSVRGYLVRPEAMAPDIRASFVHFAMTELLKRRGLYTFHATALEHK
                     GQGVLIPGFSGRGKTTSFLSLLRAGYRYLSDDHPFFRVSGARVEILPYPLKINVTDHT
                     VSFFPELREAPPSVLHAGVPKSYFHAEDVYPGPLGQPCEPSVILFPEVVNSSHSCLEP
                     LSKKVALEMILPHSLLVYDTEVARREFQALVGLVEQADCYRLHFGRDVMELPWLVTPL
                     LEKRQARREN"
     gene            7754..9049
                     /locus_tag="PK614_00740"
     CDS             7754..9049
                     /locus_tag="PK614_00740"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRB80773.1"
                     /translation="MGQFPRSTVAQWSSSRLGGISRKGTTPAPGLSLMRAEGRLLALC
                     ARTTVSEFVRVEVADLACDGIDWELVWQLSRAHGVAPLVYRNLVTICPAAVPSSIHEA
                     FRRHNQANALLNSLLAKELVTLLDALAAKGVTAIPFKGVTLAQAAYGDLGLRECADID
                     LIVEQGAIPQARKVLWSQGYQLTSQDMGREEESGEPYHFFQRRNGIVAVDLQWVMARR
                     HFGFRLDRSAFWGRLKPVHLPTKSVMGLCPEDLLILLCVHGSKHAWEQLKWVCDVAEL
                     VRRRPALDWSRVLFQAGEWRCRRLVLLGLAMAHSLFDTAVPLTILQEIETDADIPVLV
                     RKMPKQLLKNPRHGIDEDCAEALYMTLKDSWLERWKLGVALCRAEAEVLTRSLPWFRV
                     QRRLRMLATCLKPFHRIIAKWILSVRMREAVVRWLQSSG"
     gene            9239..9784
                     /locus_tag="PK614_00745"
     CDS             9239..9784
                     /locus_tag="PK614_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB80774.1"
                     /translation="MKRLLVAVAIVLVHLWAAHLVVAAGFFKVGEPAPTFSLTSVTGD
                     AVSLEQLKGKVVVLGLFHICDPCMTQGTNLQKVHEAMTGKNVAVVGVNSSGDSKRGVG
                     EFLSAFPVKVTYPYLLDPNKTTDKLYGGGKFIPNVYVIDQRGVIRWQRVGNMELAGAE
                     VIIQEVEKLLAASTPAGAGTM"
     gene            9804..10043
                     /locus_tag="PK614_00750"
     CDS             9804..10043
                     /locus_tag="PK614_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80775.1"
                     /translation="MDEWEEGKQKFLEVVQSIDQTVQVVIPTKPTNSMFLISLTKGPN
                     RKFITLSEDDIVDLPSEAGICAKVTKTLQDAVAAL"
     gene            10068..10664
                     /locus_tag="PK614_00755"
     CDS             10068..10664
                     /locus_tag="PK614_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80776.1"
                     /translation="MKQLLPGIWQWSWFSEDKQLDFNGLFLNVGEHKILIDPPPMTAE
                     AHTFVRRQGALDYIIVTNRDHVREAASYQADFRCQLQVPEADVAQMDVKPSKTFKDGE
                     LLPGGIWVIHLNDQKSPGESALYIQQGKGVLIVGDALIGKPAGGLSLLAPEKYGDITK
                     ARDGLRRLLKYNFDSILVGDGASIVFGAKQAVEQALQT"
     gene            10671..11495
                     /locus_tag="PK614_00760"
     CDS             10671..11495
                     /locus_tag="PK614_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB80777.1"
                     /translation="MALRNGLSEELNRQGNEHFARGHYTDAYACYAKALECDRLTGDH
                     RALSATLGNLGNICAVSGRRESAQSYYQEVLELQKVLGDEKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDIMTRVHDELGKAVLFSDLGLVARETGQFEEALRYYEQSLVLMRR
                     LNNQGGVADAWRMMGRTFAIQKRYDDAIACCHTSQSIAERSRDELRTGGARYVLAQCY
                     EDLGQLQMAVQLLEQVVRMDRKYDLPKLAENVTRLERLRARLAAEDPTPQPRESRA"
     gene            11492..11929
                     /locus_tag="PK614_00765"
     CDS             11492..11929
                     /locus_tag="PK614_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80778.1"
                     /translation="MNAVALPSWEQARARLVSTVPQFYELEPGGALALDLGDDGWLLE
                     VRSDGQLLCQHGIAMDDVKSLMCDGTTEDLGTDEVAKQAKYFLGPAVSKKRPALLKAG
                     FAEQTDMNDEYVAITFHKTVDFQRFDEVLAAVRWCQNLFKIQP"
     gene            12001..12858
                     /locus_tag="PK614_00770"
     CDS             12001..12858
                     /locus_tag="PK614_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HRB80779.1"
                     /translation="MAACRLPRVIVTALELSLFTAMGSGSWSIQKLAQALDVSERGVD
                     ILCRNLAMCGLLRKRGKAYRNTPFSATVLNAKHPKYRGAYVELLQSHWVDWSRLTRVV
                     RTGSPLERDQPDLLAYRRRFTWAMHHRSLDVAARVAAQLDLGQAETLLDLGGGPGTYA
                     MAFLARSPRMKATLCDRATALDVAKEIAATHRARKRLSYLPSNFMDEALAGSVWCSVV
                     FQCAPYLFRGRKPGAVSAIVFCTIPRRTTDHSGCISSRPRRHLSRRSQPVRREYAVGY
                     TCGKYLFIQ"
     gene            complement(12906..13352)
                     /locus_tag="PK614_00775"
     CDS             complement(12906..13352)
                     /locus_tag="PK614_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80780.1"
                     /translation="MGNRGSYSASMKAFRPVIALLVALVVLEGLLFEAGSANSLATIR
                     DGILLGFLFGVPTLLVAGILLLRQHWVVMAAVMYSTIALALDLSTIVQEASQASPRPP
                     ILALTLGSSLLNFLIMIFGGRCALSFRPDESPPTTPHPNLQSPSSS"
     gene            13654..14508
                     /locus_tag="PK614_00780"
     CDS             13654..14508
                     /locus_tag="PK614_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80781.1"
                     /translation="MALALWATSVLVLISGLSSAALSHVSPGSITAPGFSGKGVAQVP
                     ADHSEVLAAGFGFQAAGSSMITVRTYDAPTGAILSEDSFDVNVKEEGVAEHDGNKGRI
                     FAGGIGTDSKGKSVFMLRVYDAETGRFLWEGQLNLLKLGEGGMTRASATIIPTRTSAL
                     QVSVSSTTPLQTLFSVRAVNPFTGGLVWQDQFAPGVRKRARVEGVLFGGLFVRPVGDP
                     IGHIFDLVVRTYDRTSGTLLWEDSFEQLDRIEESPTEPELRAHPQAIPSWNLSAASRH
                     HAYQTKLR"
     gene            complement(14511..15953)
                     /locus_tag="PK614_00785"
     CDS             complement(14511..15953)
                     /locus_tag="PK614_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB80782.1"
                     /translation="MYRRNIKHILGPLAVLIGIFIFYQTWLKPRYFHDTPAPSPQVIV
                     EETTPASNAPVAPSQERTAPEIKRSRTIDPVSIPVPRHPELLGAIHEELEKHNIALAE
                     TKLEELPSSILTESATKRHVAILWNNLGIIQERSGGTEVSVKAFKKAVALDPQNPIAH
                     LNLAHAYWGLRDPALTEDFLRKVVALSPDEPFPHVALADLLQEKDHLPEAGKHLAQAK
                     ERIKKDPGLQSYLKAVTTKVHRAEKVEDKFSTHSSVHFTVKYDGGDDPDTWTTVLDIL
                     EEAYREIGQKFNFFPSKPIIVVLHTKNQFQGATGSPGWADGLFDPVLGRIQIPTQGAA
                     TDRAWLTRVLRHEFVHALIHEELGATGGMIPTWLNEGLAMQLAGDPWQEVTSVTPGEH
                     SLVPLTALEGSWESLPAEKVSLAYREANAATHYLIERFGMHKVHEVLVHLKARQTIAA
                     AMQDRLLLSYDHFQQQWADSLGSTLAQPKS"
     gene            16089..18656
                     /gene="recG"
                     /locus_tag="PK614_00790"
     CDS             16089..18656
                     /gene="recG"
                     /locus_tag="PK614_00790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRB80783.1"
                     /translation="MAVVRSCSRMPRPESADSHDPFQAFLHRVTRPIEFACRDAYAHL
                     SAVRNLDRFVSQQVIGTLGERVYPRALETELIALRNLFVDFHTRLTPLEQQDRLTKAL
                     ALLSRLQGDARAVSQAAGSPKENQVQPPPVRSSPARPLWELSIQYAKGVGPKRTLLLE
                     RLGAHTVEQALWTLPWRYEDRSVITPVAELVPGATRSVCGVITRAEATRARVRRLSIL
                     DVAVQDATGTVHAVFFNQPYLEDVLKEGLRVMMSGRVAAGRGGWTDVRLEATQFEVLS
                     GGEDELLHVGRIVPIYHETKGWTSRQMRVLMQGLLAEYGAGTEEVLPLSVRARHRLPP
                     IGEAIQHVHFPLPKTDLAALDQGVTSAHRRLAFEELFLLQAAMVLRQREMKEELKSFR
                     FNPHVAQLKQLAKILPFTLTSAQERVFREIQADMVTSRPMNRLVQGDVGSGKTVVALH
                     ALVMACGSGCQTALMVPTEILAEQHYLNLAPLLRAVGLKAVLLTSGGKAKDRNETLKQ
                     LESGAAQIAIGTHALIQKKVRFANLGLVIVDEQHKFGVLQRKTLLEKGYRPDVLVMTA
                     TPIPRTLAMTVYGDLDVSVIDMLPPGRKPVRTLLYTDGQRHKTWQLVGEELKAGRQAY
                     IVYPLVEESEKVDLKAAIQGAEHLQREVFPQARVGLLHGRLPTAEKERTMAAFKAGTI
                     QVLVATTVIEVGVDVPNATVMVIEHAERFGLAQLHQLRGRVGRGAHQSLCILMATYLA
                     REARTRVSRDGRPEQNASNAQQRLAALVNSNDGFVIAEEDLRIRGPGEFLGLRQWGVP
                     EFRAANLVRDAQLLEQARQEAVTLLEEDPGLMLPQHQAFKAAMFRRWRGRLALGDVS"
     gene            18694..19020
                     /locus_tag="PK614_00795"
     CDS             18694..19020
                     /locus_tag="PK614_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80784.1"
                     /translation="MGMLQRLSHDLRAGWVTLRHGTAKAATRALEEGELLRYRLELRK
                     VDQQLDDLHGDIGERTIALHDRGESADRILSDSEITRLMQQVRTLQDEHTKLLLEMND
                     ITVEGQ"
     gene            19031..19981
                     /locus_tag="PK614_00800"
     CDS             19031..19981
                     /locus_tag="PK614_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRB80785.1"
                     /translation="MIPETTAPTRIFAGIDIGTLTCRLLIAELTSAGGLRELRSDRRI
                     LRLGQGVDRERVLRSDAMARVVATLKEWRQVIDGYHVDAATAVATSAVRDAGNRDEFL
                     GLVQREAGFQVEIISGEEEARRTMLGIRSGLPSGVTDLLALDIGGGSTEFILDRPGQA
                     TLARSIDIGVVRLSERILHSDPPTPDEVQEARDWVRRETTAAIAAMPRSPGVMFVGTA
                     GTITALAAMVQQLPAYEPARIHNYTLTLNMVKQLELQLLSCSKAQREGLPGLERGRED
                     VIAAGAVILGTVMDTLGMTKVLVSDLGLREGVLIHLAGGR"
     gene            complement(20149..21642)
                     /locus_tag="PK614_00805"
     CDS             complement(20149..21642)
                     /locus_tag="PK614_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB80786.1"
                     /translation="MKAAAAIQEPLTLSGDTLSLLAWRIPDLVAYQHAFDEWWLAPLD
                     DDFPLVRLNAVGIEMLTSMNGHITVGALLEKYGNKICGPDGQPGTWHLARWSTPNYSL
                     CYFGTEPPGGHRHKAKWDLLLQQIREGWSGQQEFEGEEHLEDFHVHELKESDLEDGHF
                     DLIETTVSHLFREPCEALGGTTYGRLLMRQLRRLGWFKPKPKVIVEIGGGLGYVAREL
                     GQELLPFEKQGIQYISLDVTRPFLQLQTKRAKAGGWTGTGTHANGECLPFRDNSVDLI
                     IDNENMADMTPVKLSRKELTEGTGETAQHQEALDWIRRLRLPLEKDLPEEVIFNLGPM
                     RFVAEVWRVLKPGGRAFLTEFGVEEGWPAAVKLPHHTEYEVQYNHLRQAVRWLGFQER
                     YLSLPQFLQIKPDTKVLCTGAAYTIQRFCQGLGQNFSVRAYTESELTKTLGDILPKLQ
                     GCHYHDIADPAWFGLIDFKVLLLEKPGGIPQPTFTEQKSGLRWYSQR"
     gene            complement(21724..22098)
                     /locus_tag="PK614_00810"
     CDS             complement(21724..22098)
                     /locus_tag="PK614_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRB80787.1"
                     /translation="MSWRLFCFVLATSTLIAWPTSATPPATQVLMDSGSPYYVPAAVT
                     VTAGGAIRWENPTPTHHTVTHDACLEESGRCAFDSGAVEPGGAYTIPSLPPGRYPYQC
                     RIHPIMRGMIIVTESPLLPSQT"
     gene            22407..22994
                     /locus_tag="PK614_00815"
     CDS             22407..22994
                     /locus_tag="PK614_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80788.1"
                     /translation="MAKKIVKKKSVAAKASRPKKVAAKKATKPKKAVAAKKPVKAKKA
                     VKGTPAAKKAVKPAKKMTKKPAAAAKRPSKAKPVSKAVKPAPKAVKPAAKPKPKPAPP
                     KVKPPVEQSAVIPESKPAKPPVPEVSKAAKVEPEPQPLELDDEEEMAEDLEDVSEEIE
                     EEIEEELGLDGEDDDVDYLEKSEDLLDDADEYRNN"
     gene            23210..23662
                     /locus_tag="PK614_00820"
     CDS             23210..23662
                     /locus_tag="PK614_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80789.1"
                     /translation="MSHFLFIAGALRERTSQESAELQFGAGIWGLCTALIRDNLQKFL
                     VPDSHGLVYVLKEGIRAEFAISSPVQPFQALDELLKDEVRTEARYGFVRITPLRRWQV
                     HAPDCQALLQRVLGIEEQAELTRRLSLGMHRLTDAEFRAIVEGLGVEA"
     gene            complement(23691..24296)
                     /locus_tag="PK614_00825"
     CDS             complement(23691..24296)
                     /locus_tag="PK614_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80790.1"
                     /translation="MLLASIRLACAESVVEVPMLAAIQANNRGVFEVLLLRWDHRPSP
                     SPIELQWQGGNIRPGQTNLSSMVLAFQYALDHTPQIDQAGTVSAMGIAYAATGTDGPS
                     AGAVMTVGFAALLKGDHIRRGIALTGTIAKDGTIGPVGGIPDKIRAAAREGYRTILIP
                     QGQRDDPRWNLNRLAWELNVEVTEVATVEEAYRLMTGGTLE"
     gene            24577..25563
                     /locus_tag="PK614_00830"
     CDS             24577..25563
                     /locus_tag="PK614_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRB80791.1"
                     /translation="MKNAAAVFTLHLPNTCTEAEREARAVPAQELLRALTAGKGLDLS
                     GVVIQGDLVLDELPAQKASAVGDLAPEDRRVLEGLNDEEVHVIRGPFVIKQSRVKGRI
                     VNRLKSGFLLITGPVVLAHTDFAGFVDLSRTVFLGLVDGSNATFHQESYFVQDRFTQG
                     AMFSDTHFGPHARFHRSVFAGPAIFRGATFQGLTEFLEVVFEQDANFSRAAFHLGTGF
                     SGAHCRAKCDFSSSQFDREAFFLFAIFDRPVTFASARFGSQADFSDAAFKQADDLAQA
                     TFARTPQLTRTARVSTTVPGPTASASAPFSQAVTILLFVMALGLLVYIIRAK"
     gene            25662..26441
                     /locus_tag="PK614_00835"
     CDS             25662..26441
                     /locus_tag="PK614_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRB80792.1"
                     /translation="MDQPELPYQVKIENFEGPLDLLLHLIKKNEINIYDIPIALIAQQ
                     YLDYLSVMKELNLAVAGEFLVMAATLLHIKSRMLLPVDEVAVDEEDGPDPREELVRRL
                     LEYKQFKEAASQLDYKERLWRDVFSREPGPPVEVKKDESLLDEISLFDLVDALQGVLS
                     RHPGKRLVEIIPDNLTVRTRMSAIIEALELHESMAFTALFEESSHRLVVIVTFLALLE
                     LIRIRVVRVFQSETFGAILVSRAFSAVAEGDLVEEPEWKNL"
     gene            26426..27343
                     /gene="scpB"
                     /locus_tag="PK614_00840"
     CDS             26426..27343
                     /gene="scpB"
                     /locus_tag="PK614_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRB80793.1"
                     /translation="MEEPMSPGVEEAIEESIEVRDPVDEPTASLDAVEVEPEVGQDVI
                     TEMSGPAADPALSDMRALKGILEALLFVTAEPIPVTRFLALLGAVTKQDVDQALASLA
                     HDYEQEGRGLQLAEVAGGYRIVTKAEFAPWLKRLEKVKSPSKLSRSALESLAIIAYKQ
                     PIVRAEVEQIRGVETSGVIRTLLERKLVRIVGRKEEPGRPIMYGTTKFFLEHFGLRDL
                     SQLPPLREFKELGESEQAMLPIDDVVAVEGEPLLQVTEASGEGLSEDMAEPVALLEPG
                     DEAGQTSELLVLENEAGGDLVVADIAEES"
     gene            complement(27513..28079)
                     /locus_tag="PK614_00845"
     CDS             complement(27513..28079)
                     /locus_tag="PK614_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80794.1"
                     /translation="MWPTRLVLATLAASLLLTPSVHAVESSPVEMWECPGPEGTILYT
                     NKERPDCQAKVLKALSVVPSLPDLPLAPPSGSTYQPTPNAKDFSYDTPIGALRNLSQI
                     PESGRDWYAGNASGGSVQGEVCSMYLEWIHLNQNSRGGHFFGTDPSYGSNPTARNWYA
                     PSYSFYDNARYVTLSQIFGAGFVPIGCH"
     gene            28406..31375
                     /locus_tag="PK614_00850"
     CDS             28406..31375
                     /locus_tag="PK614_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRB80795.1"
                     /translation="MIKAWLLDEMFRFDRRYLTAEGSQSEWGAGDSVAEEEDLPPAPT
                     GVAAQAGNGRVMITWDAVPEAMYYNLYFMTTKGVQIKFSELTRPIASAEDFKTVIGVT
                     KEKGTCIEGAQSPFMHDDLANGTCYHYVVTVVTQKGESPESQEVMAIPAPYLIAKIIG
                     QEGVEDGEFSSPTGIAVDKGGNIYVADTDNHSIQKFDKDGKFLGRWGGEAGSAEGGFY
                     YPRGLTTNSDGQVYVADTGNNRVQRLDTDGNPMKAWGKFGFAWRGADMNKFDAPWGVT
                     TDQDGNIYVTDTNNARIQKFKGDGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNTYVAD
                     EGNNRIQKFDTRGSFLTKWGREGSGPGQFKAPWGVTCDALGNVYVVDQGNHRIQKFDG
                     NGTFLCAWGNRGKTEGQLNFPSGVAVDKEGAVYVVDSGNHRVIKYVPTDEELNRGKAE
                     RERAQAVSEYPIPRNLAIKPGDTETFLSWMDVPGAVSYNLYFHTSANLTQEGGTKIEG
                     VTSPYNHSGLTNDQAYYYALTAVYEDGTESGLSDEVSATPVLIDITAPQTPYAVINHG
                     AFMTNSPEIVVTISATDLDTGVAAYYISENPMTPMAGTPGWVEVPPAIKFGATIPFIL
                     SPGDGQKTVIVWFKDLGNNISTPASATILVNTSGYLCVSKWGKPGRGASLLHGGEFMA
                     PMYGLAIDQQGSIFVVDNGNNRIQKFDRTGNFIILWGNFGAANANFHNPTGIACDAKG
                     DVYVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNAPWGIAIDRVRGYIYVVDSAN
                     FRVQKFDMSGEFIMSWGSFGNGDGQFYFPRGIAVDQADGTVYVVDMGNHRVQKFDTST
                     NVLPQLLTKWGGSSEAGHASSPLAQEAGQLRSPWGIAVDGQGDVYVTDTGNHRVEKFD
                     REGNFIAQWGGFGGGDGQFNFPYGIVVDSRGSVFAVDSGNTRVQQFMPADEGSERLQE
                     EAEAAAELGQVDKTTRV"
     gene            31414..32880
                     /gene="guaB"
                     /locus_tag="PK614_00855"
     CDS             31414..32880
                     /gene="guaB"
                     /locus_tag="PK614_00855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRB80796.1"
                     /translation="MLEKEIRLGLTYDDVVLVPAKSQVLPSEVDTRTRLSRNIQLNIP
                     IVSAAMDTVTEARLAIAMAQEGGIGIVHRVLSPTDQAAEIDKVKKSESGMILDPITIS
                     PDQTIRDAHDLMARYRISGIPVTKAGKLVGILTNRDLRFETRMELKVSQVMKRDKLIT
                     APEGTSLEKAREILHEHRIEKLPVVNKQFELKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAALGVGPDTADRVALLVKAGVDVVVVDTAHGHSQAVLDTVKMVRKAHPKLDIIAGN
                     IATAQAAKDLVKAGVDAVKVGVGPGSICTTRMVSGAGMPQLTAIADCARALAGSGVPV
                     IADGGIKYSGDITKALAAGASVVMLGSLLAGTEEAPGETVLFQARTYKVYRGMGSIGA
                     MERGGGDRYGQGGRPTPKLVPEGIEGRVPYKGTLAPHIYQMVGGVKSGMGYCGCKTIP
                     DLQQKATFIRQTVAGLREGHVHDVIITKEAPNYRTDWE"
     gene            32972..34528
                     /gene="guaA"
                     /locus_tag="PK614_00860"
     CDS             32972..34528
                     /gene="guaA"
                     /locus_tag="PK614_00860"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRB80797.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATV
                     LAYRPKGIVLSGGPSSVYDKKAPSVSKQLFEQGIPILGICYGMQLVTHLQGGEVEKAP
                     HREFGRAELTLDDRSDLFKGIGTRGSTVVWMSHGDRIERMPPGFRSIAHTDNSPIAAM
                     KRHDARQRIYCLQFHPEVAHTTEGATILKNFVYDICGCKPTWTMRSYVDTAVEQIRQQ
                     VGTERVICALSGGVDSSVAAALTHRAVGDQLTCIFVDNGVLRTGERDQVEKTFASQMH
                     LNMRILDRTSQFLTGLKSVTDPERKRKIIGRLFIKNFEVESKKLKGIKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPTRMKLKLVEPLRELFKDEVRVLGQELGLPDEIIW
                     RQPFPGPGLAIRVLGAVTKERLAILRGAEMIVDQEIRAAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYEYVIAIRAVTSLDGMTADWAKIPSDVLGKMSSRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            34624..35886
                     /locus_tag="PK614_00865"
     CDS             34624..35886
                     /locus_tag="PK614_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB80798.1"
                     /translation="MEVRLQKLIAGTGLASRRKAEELITSGRVTINGSVVKELGTKVD
                     PARDHVKVDGKHLRAAQPYVYLILNKPKNVMSTLDDPEGRPTVKDLLHGVTVRVFPVG
                     RLDFDSEGLMLLTNHGDLAQALLHPRYHVPKTYLIKVKGVLDDAKIEALERGVKLEDG
                     FTAPAKVNKVSKAESNSWLEVTIHEGRKHQVKRMVEAVGHSVIKLVRVRMGPLLLGDL
                     APREYRFLTDREANRLRDLVEDRVARAERPELDASGKPTRWMPPTEPAPPRPPKPERS
                     GRGPKAQRSERGPRSQRSLAGAKPSRSGRGTTRRPMSPGAKFRRPAKGVGFQRTQAGP
                     IPQGADRGPGSRPTGPDQKPQRTQPGAGFQRPRSGAKPQGPGRGMTVRRSGPGQNFQR
                     KRRGGSSASRPGQRGPAGKSRPSTRRRA"
BASE COUNT         7474 a         9342 c        11269 g         7814 t
ORIGIN      
        1 gtgttgctgc cgtttttcaa agaacgccgg atgatgctcg tcgatttcaa gttggagttc
       61 ggcgtctttc acaatcgact catcctcgcc gacgaaattt ctcccgatac ctgccggttc
      121 tgggaccaga cgaccaaaga gtcgatggac aaggaccggt tccggaagga tttgggcaaa
      181 atcgaagagg cgtatcagga agtattgaag cgggtatgcg aataggggcg gcatgaaccg
      241 gtttctccag ttgtttttga actatggtct ggtggcggcg attctggtgt gggcggccac
      301 ggtggcgttg atggcctatc acctcaagga atccccctgg cgctgggcgt ttgtcctgat
      361 gtcgctggca gggctgggga ccatcggcgt gatcttttgg atcaggaagt acgtgcagag
      421 ggtcggtaag gcacagcagg aagtgggaaa agcacaatga aagccaagat tcatgtgacg
      481 ctgaagcagg gaattctcga tccgcagggg aaggcgatcg aacatgcgct ggactccctc
      541 gggtttaaga atgcgggaaa cgtgcgggtc ggcaagtaca tggaagtcga cgtgaacgaa
      601 gcggaccgcg ccaaggcgga tgcccaggtg aagcagatgt gtgaaaaatt gctggcgaat
      661 acggtgatcg aggattatcg gtacgaactc ggataggagc agatggctta tagcctatag
      721 ctgatggcag aggccgagac ctcgttcctg tctcgtatca tacgccattt gccataggcc
      781 ataagctctt tagaattatg aaaatcggcg tggtggtatt tcccggcagt aattgcgatc
      841 acgactgcca gtatatcttc aaggatgtgc tcggccagta tgtggagatg atctggcata
      901 aggaaactct gctcgcgggg ctcgatgcga tcgtgttgcc gggcgggttt tcctacggag
      961 actacctgcg gacgggcgcc atcgcgaggt tttctccggt gatgggagcc gtgaaggcct
     1021 ttgcgaataa gggtggcctg gtcatcggca tctgcaacgg gtttcagatt ctgctggagg
     1081 ctggtctcct gccaggtgtg atgctgcgca acacctcgct caattttatc tgcaaagacg
     1141 tctacgtgaa ggtggaaaat gcggcgacac ggtttacgaa tcaatgcgag tccggccagg
     1201 tgctgaagat tcccatcgcg catgccgacg ggaactatta caccgacccg gtcacgctcg
     1261 gcggcatcaa agccaatgca caagtgatct ttcgctactg cacgggagac ggcaaggtga
     1321 cgcccgatgc gaatccgaac gggtcgctgg ataacatcgc cggaatcatg aatgcggatg
     1381 gaaatgtgct gggcatgatg ccgcatcccg aacgcagtgc agaagcgata ttgggcaatg
     1441 aagatggtcg gctgatcttt cagtcgatgt tgagctcgtt cggcaagccc gaattgcaat
     1501 ccaagttgat tggagtatga cggccggttc gcgtgagcag cggtgccgga actgtcggta
     1561 accccctacg attgagaatg tgtcattcat gaacgcactg gtcattacca aagccatcct
     1621 tgagcagcat aagctgagcg atgatgaata taagaagatc ctggagatct tggggcgaga
     1681 acccaactgg accgaactgg gcatgttctc ggccatgtgg agcgaacatt gttcctacaa
     1741 gagctcccgc atccacttga agaagttacc gacgacgggg ccgcgcgtgg ttcaggggcc
     1801 cggcgaaaat gccggcgcgg tggatatcgg ggatggtctg tgcgcggtgt tcaagatgga
     1861 gtctcacaac catccttctt tcatcgagcc ctaccagggt gcggcgacgg gggtgggtgg
     1921 aattctacgc gatattttta cgatgggcgc tcggccgatt gcgttgttga actcgctgcg
     1981 attcggcgga ttggacaatg cgaatacccg ccatctgctc aagggtgttg tgtcgggtat
     2041 tgccggttac ggcaactgca tgggtgtacc caccgtcggc ggggagattg tcttcaacga
     2101 catctatgcc ctgaatcctt tggtgaatgt gttttgtctg ggaattgcca agaaggataa
     2161 gatttttcta ggcaaggcgg ccggcgtcgg caacccggtg atctatttcg gttcgaagac
     2221 cgggcgtgac gggattcacg gtgcgacgat ggcctcggac tcgttcgatg aacaagcggc
     2281 tcaaaagcgg ccgacggtgc aggtcggcga cccctttact gagaagttgt tgctggaagc
     2341 ctgtttggaa ttgatggccg gtgattgcct ggtcggtatt caagacatgg gtgctgccgg
     2401 tttgacgagt tcggcctgtg aaatggcctc gcgtgaaggt acgggcgtgg agttggaatt
     2461 ggcagatgtg ccgcgtcgtg aaccggggat gacgccctac gagatcatgc tctcggaatc
     2521 ccaggaacgg atgttgatgg tggcgaaggc cggtcgtgaa gacgaggtga tcaagatctg
     2581 ccgtaagtgg gatctggatg tggccgtgat cggccgagtg accgatacgg gcaaggtcgt
     2641 cctcaaagaa aacggccaga tggtggcgga gattccggcc aaggctctgg ccgacgacgg
     2701 tccgcgatat gaccggccta gtgcgccgcc tgcctatcaa gaaatgttgc aggcgctgaa
     2761 tcacgatgcc ttgccggacg tcaaggacgt taatgccgca ttgctcgcac tcctggattc
     2821 accgaccatt gccagtaagc gatgggtcta tgagcagtat gaccacatgg tgagaacaaa
     2881 tacgatggtg cgtcccgggt cggatgcggc ggtattgcgg gtgaagggga cgaataaggc
     2941 gctggcattg tccgtcgatt gcaacgggcg ctattgtctc ttgaatcctt atgagggcgc
     3001 caagattgcc attgtggaat gcgcgcgaaa ccttgcctgc tcgggagctg agccgatcgg
     3061 cgtgactgac tgcctgaatt tcggcaatcc gcagcgcccc gaggtcatgt ggcaattcgt
     3121 acttgcgatt gaagggctca aggatgcttg cgaggcgttg aatgtgccca tcgtgagcgg
     3181 caatgtgagt ttgtacaatg agacgaatga cctgtcgatt taccccacgc cgatgatcgg
     3241 gatggttgga ttgatcgaag aggccgatcg tgccgtgacg cagtggttca aggaaagcgg
     3301 cgatgtgatc attctcctgg gccagacccg tgaagatctc ggtggaacgg aatatctgaa
     3361 ggtcttgcac catcgagagc agggttcgcc gcctttgctg agtctggaaa ctgaaaaagc
     3421 cgtgcaggct tgcacgattc gactcatccg cgatggattg gtgcagtcgg cccatgactg
     3481 ttccgatggt ggattggcgg tggcgctgac ggagtgttgc atctcgggtt cagctcaaca
     3541 actgggggct gtggtacaat tgtcgcttga tggtcttcgt cgagatgcgc tgctctttgg
     3601 ggaaagtcaa tcgcgaattc tgctctccgt gaaagcggag ctggccgagc gggtcttaaa
     3661 tgcggcctgg gatgccggtg ttcatgccgc tcggatcggt acagtcggag gtgcccggtt
     3721 ggtgattcaa gtagacggtg atcagcgggc agctggctgc aaaatcgatc tcgaattgac
     3781 ggcactgtat gcccattggg gattggcaat ccctcgtgca ttaggtcagg actaggatac
     3841 ggacgtcatg gccaacgaat taccgctgat ttctcccgat aagttccacg acgaatgtgc
     3901 cgtattcggt atctacggcc ataaagaggc cgcgaacctc gcctacctcg gtctctatgc
     3961 gctgcaacat cgcggacagg aagcgtccgg cattgtctcc aacgacggcg aacagtttta
     4021 tgtcgaaaag gggcagggtc ttgttgcaga tattttttcg caacaggcct tggcacgtct
     4081 gcccggcacg atggcgatcg ggcacaaccg ctattccact gcgggcgggg caggcctcaa
     4141 gaatgtgcaa cccttgagcg tcaattttgc cttcggtaac ctcgccgtgg cgcacaatgg
     4201 gaatttgatc aatgccacca tgcttcgcag tgaactggaa gcctatggcg ctatttttca
     4261 atcgacgtcc gacacggaag tcattatcca tctcatcgcc cattcgcggg cggatacgct
     4321 actggaccgg gtcatcgatt cgctcaccca ggttcgcggc gcattttcag tggttctgat
     4381 gaccgatcag ggcattgtcg ccgcgcgcga tccccatgga tttcgccccc tgtgcctggg
     4441 acggtttcgt gacgcctgga tagtagcctc agaaagttgc gcgttcgatc tgttggatgc
     4501 cgaatatgta cgggagatcg aaccgggaga gttggtggta ttggatcatc agggcgtcac
     4561 cagttataag ccattcgctc aaaccaagcc ggccatgtgt gtgtttgaat atgtctactt
     4621 tgcccgtcct gatagccgca tttttggagg caaagccgtc tattccatcc ggaaggcatt
     4681 tggccggcaa ttggcgcaag aatcgcgggt tgatgcggac atcgtgattc ccgtaccgga
     4741 ttccggggtg ccggcggcgt tgggttattc ggaagggtcg gggtttcctt ttgaaaccgg
     4801 actgatccgg aatcattatg tgggtcgcac cttcatcgag ccggagcagt caattcgtca
     4861 cttcggggtg aaggtgaagc tcaatgctgt tcccgaagtc ctcgaaggga aacgcgtggt
     4921 cgtagtagac gattcgttgg ttcggggaac gacgagccga aaaatcgtca agatgctgcg
     4981 tcatgccgga gccaaagaag tgcatatgcg catcagttct ccgccgatcg tgtcgccctg
     5041 tttttatggc atcgatacac cgaccaagaa ggaattgatc gcatccagcc ataccaccga
     5101 agagattcgc aaatacatca ccgcggatag cctcgcctac ttgagcctgg acggcatggt
     5161 gaaggcggct cccggcactc cggggcagta ctgcgatgcc tgctttaccg aacaataccc
     5221 tatctccttt acccgggcag aagaactcca actagggctc tttgaagctc cacgctgata
     5281 tcgttgtcct cgccggttca taggatcgtc tggacttcct cctgcgcctt gctagactaa
     5341 ttggcatgtc gctggttcgg aagtatcgag tgatcgttgc cactggtgtt gtggtcgttg
     5401 gggtccgcat cgcgtcccgc ctgacgagtc tcaggcgatt gctcggctgg ttattatgcg
     5461 agatttcagc cgatccggat gagcacgcga tggaagatgt ggcctattat gtggatcggt
     5521 ggctcgcgct gtttccatac aatccgaagg gaaattgttt tcctcgagcg ttagcgctat
     5581 tttttttcgc ccgccgtgcc ggcctccccg ttcaattcaa atgcggagtg atgaagctca
     5641 atcagcagct ggagggacac gcatggctgg tgctggaggg gcatgagttt cttgagccgt
     5701 cctcctactg gcaatcgttt accgtgacgg ttaccttccc tcaatccctg gtttcctcaa
     5761 gtactcaatc atctcgcatc tcctgagacc tcctctgcac attaccgttc tcttggggtc
     5821 aactggacct ggcgggcctg ggcgaatcga gtctttcctg tgcgtctgcg tgaatcaagc
     5881 tgacaagttc ggctttgaaa cccactagcg gccccatccg cacgcttttt catccgtttt
     5941 cataaaaaca aaatccgaat cccccaaagg gaggtggagc ccaatctcac tggggataga
     6001 gaaacgagcg tgatgacgat agtttcgccc ttgtcttgca gtgatgtgta cggtctgctt
     6061 gtggggggac caagacttcg ggggaaaatc ggttttagaa cgaatgatgg tatcggagca
     6121 gtcacatgtc cccagcaatc tgaaccagga gcatctatat gcctgccact gtgcatctgt
     6181 ggagtgagcc tcagcttgaa ttgtccgaaa tgtttcccag gcaggatgaa caggtccaca
     6241 gcacggttct cgatggtgaa agtgtgctgt tgaatctgaa ttccggccgc tattacacgc
     6301 tcaatgtcgt cggatcaacc atttgggatt tgtgcagagg agagcagtcg ttgcggcaga
     6361 ttcactcagc aatctgtgcc aggtttgatg tgtctgaaca gcaggcgcag gacgatatgt
     6421 tggcgttgat tgtgcttctg gagcaggaag gattacttca cacagaaagg aggtgagtga
     6481 aaatggagca acagataaag accgaatacg tcaagccggc gctcgtgaaa catgagttgc
     6541 tgcgcgacgt aacggccatt cagttgtccc gcgtgctgga tcgaacaccc gtataacatg
     6601 ccaggtgctc tagtgagagc catggagctg gccggttaac tagaccggcc agctcgcccc
     6661 acacctctgc ggtagtgacg acgggcagaa ggctgaaaag gagaagcggt aatgttcatg
     6721 gcgtcgacaa tgaaggaact tctactctcc gtccatggca tagagatgaa attcaccacc
     6781 aattcgtccg agttggcaca ttccgtttgg gcgttactcc gtcactttca ccgcgacacc
     6841 gttgcggctc agcccatgcg gatggagttc gacgcgatcg agcgacgtga ggatgttccg
     6901 gtccagattt ccagttcagc gaaattgttg tacagcggtt ctggcctcgc gatcgaccct
     6961 gatcggaaga cgacatggtt gtgcgacatt tattcagaca acggcctatt gattgccgat
     7021 tttcatggag agggccttgt cgtgattgat ggggactcac aatctgtccg gggctatctg
     7081 gtgagaccgg aggccatggc gccggatatc cgggcgagtt ttgtccattt cgccatgacg
     7141 gaacttctga agcggcgagg attgtatacg tttcatgcca ccgcactgga gcacaagggg
     7201 cagggtgtgc tgattcccgg tttcagtggg cgggggaaaa ccacctcgtt cctatccctg
     7261 ctgcgagcag ggtatcgtta cctttccgat gatcacccat tttttcgggt cagcggcgcg
     7321 cgtgtggaga tcctcccgta tccgctcaag atcaatgtta ctgatcacac agtatcgttc
     7381 tttccagagc tgcgagaggc cccgccatct gtgcttcatg ccggtgtccc caagagctat
     7441 ttccatgctg aggatgtata tccggggcca ttaggtcagc cttgcgagcc cagcgtgatc
     7501 ctctttcccg aggtggtcaa ttcctcgcac agttgtctgg agcccttgtc caagaaggta
     7561 gcattggaaa tgattcttcc gcattccttg ctggtgtatg acaccgaagt ggcgcgccgg
     7621 gagtttcaag cgcttgtggg attggtcgag caggcagatt gttaccgact ccacttcgga
     7681 cgcgatgtca tggagttgcc atggctggta acgcctcttc tggagaagcg gcaagcaagg
     7741 agagagaact gacatgggac agtttccgag gtccaccgtc gcgcaatgga gctcctctcg
     7801 tcttggaggt atttcgcgta aggggacgac accggcgcca ggattgtccc tcatgcgtgc
     7861 ggagggacga ttgcttgcac tctgtgcgcg aacgacggtg agtgagtttg ttcgagtcga
     7921 agtggcagat ttggcctgcg atggaattga ttgggagctg gtctggcagc tgtccagagc
     7981 ccatggcgtg gcgccgctgg tgtatcgcaa tctggtcaca atttgtcccg ctgccgtgcc
     8041 ttcttccatt cacgaagcct ttcgtcggca caatcaggcg aacgctcttt taaatagctt
     8101 gttggctaaa gaactcgtga ccttactcga tgcgttggcg gccaaggggg tgacggcgat
     8161 tccctttaaa ggcgtcacgc ttgcacaggc ggcgtatggt gacctaggtt tacgggaatg
     8221 tgcggatatt gatttgatcg ttgagcaggg agcgattccc caagctcgga aagtcttatg
     8281 gtctcagggg tatcaactga ccagccagga tatgggacgc gaggaggagt caggggagcc
     8341 gtatcacttt ttccagaggc gaaacggcat tgttgcggtc gatcttcagt gggtgatggc
     8401 tcgccggcat ttcggatttc ggcttgatcg gagcgcgttt tggggaaggc tgaagcctgt
     8461 tcacttacca acaaaatcgg tgatgggcct ctgcccggag gatctgttga ttcttctttg
     8521 cgtgcatggg tcaaaacatg cctgggagca gttgaagtgg gtctgcgatg tggcggaact
     8581 cgtgcgtcgg cggccggcgt tagattggag tcgtgtgttg tttcaggcag gcgagtggag
     8641 atgtcgacgg ttggtcctgc tcggcctggc gatggctcac agcctgttcg atactgccgt
     8701 tccactgacg atacttcagg agatcgagac agatgcagac atccccgtcc tcgttcgaaa
     8761 gatgcctaag cagctgctga agaatcctcg tcatgggatt gatgaagatt gtgcggaggc
     8821 tctgtacatg acgctcaaag actcctggct tgagcggtgg aaacttgggg tcgcgctctg
     8881 tcgcgcggaa gcagaggtgc tcacccgttc gttgccctgg ttccgggttc aacgacgact
     8941 tcgtatgctg gccacttgtt tgaagccgtt tcatcggatt atcgccaaat ggattctttc
     9001 tgtccggatg cgtgaagcag tcgtgcggtg gttgcagagc tccggctgat cgtgggtttt
     9061 ccggcatgaa acgtttctcc ttcgtcaata gataaatggc ttgcctccct aagtggttgc
     9121 tcccgaaaaa aaacgcatgc taggatacct gcatcaccag cagtgattgg tgactgctga
     9181 gaaccagccg agggagcatt ggcctccatc attggcttgg ttccaggaag gaggctctgt
     9241 gaagcgactt ctcgtagcgg ttgcgattgt attggtgcat ctgtgggctg cccaccttgt
     9301 agtcgccgcc gggtttttta aagtcggcga gccggcgccg accttctcgt tgacgtctgt
     9361 gacaggcgat gctgtttcgc ttgagcagtt aaaggggaaa gtggttgtcc tcgggttgtt
     9421 tcatatttgt gatccctgta tgacccaagg gaccaacctg caaaaggtcc atgaggcgat
     9481 gaccgggaaa aatgtggccg tcgtcggggt caattcgtcg ggggattcca aacggggcgt
     9541 gggcgaattc ttgagtgcct ttcccgtgaa ggtgacgtac ccctatttac tcgatccgaa
     9601 taagacgacc gacaaactgt atggcggtgg gaagttcatt cccaatgtgt atgtgatcga
     9661 tcagcgtggt gtgatcaggt ggcagcgggt gggcaatatg gaattggcgg gcgctgaggt
     9721 catcattcag gaagtggaaa aactgttggc agcttcgact ccagccggcg caggaaccat
     9781 gtagtgggga aagaggatca tccatggacg aatgggaaga gggaaagcag aagtttctgg
     9841 aagttgtgca gagtattgat cagaccgttc aggtcgtgat tccgacgaag ccaacaaaca
     9901 gcatgttcct tatttccctc acgaaggggc cgaatcggaa gttcattacc ctctcggaag
     9961 acgatattgt cgatcttccc agcgaagccg gcatctgtgc gaaggttaca aagactctcc
    10021 aggatgcggt ggcagcgttg taatcccatc cacgtgaggc gatatctgtg aaacagctcc
    10081 ttcccggtat ttggcaatgg tcctggtttt cagaagacaa gcagctcgat tttaacgggc
    10141 tgtttctcaa cgtgggggaa cataagattc tgattgatcc tccgccgatg acggccgagg
    10201 cccacacatt tgttcgacgc cagggtgccc ttgattacat cattgtcacc aaccgtgatc
    10261 atgtccgcga ggcggcatcg taccaggccg attttcgttg ccaactacag gtgcctgagg
    10321 ccgatgtcgc gcagatggat gtgaagccgt ccaagacatt caaggatggc gagttgttgc
    10381 ccggcggtat ttgggtcatt cacctgaatg atcaaaaatc tccgggtgag tcggcgttgt
    10441 acattcagca ggggaagggc gtgttgatcg tcggcgatgc gttaattggc aaaccggcgg
    10501 gaggcttgag cctcctggct ccggaaaaat atggcgatat cacgaaggcg agagacgggc
    10561 tccggcgatt gttgaaatat aacttcgact cgattcttgt aggcgatggc gctagcatcg
    10621 tattcggagc taaacaggct gtagagcagg cgcttcagac gtagggaaca atggcccttc
    10681 gaaatggact ctcagaggaa ctcaaccgtc agggcaacga gcattttgcg cgcgggcact
    10741 atacggatgc ctacgcctgt tatgcaaagg ccctggaatg tgatcgcttg accggtgatc
    10801 atcgcgcttt gagcgcgacc ttgggcaatc tcggcaatat ttgcgcggtt agcggtcgcc
    10861 gtgagtcggc tcagagctac tatcaggaag tgctggagtt gcagaaggtc ttgggtgacg
    10921 agaagggcat tgggacgacc ctggccaatc tggggaatct ccgcgccgac gctggggagt
    10981 gggatcgtgc gagagcctat taccttgagg cgcttgacat catgacgcgg gtacacgacg
    11041 agctgggaaa ggcagtgctg ttttccgatc tcggcctggt ggcgcgtgaa acaggccagt
    11101 tcgaagaggc actacggtac tatgagcagt ctctggtgct catgcgccgg ctcaacaatc
    11161 aaggaggcgt cgccgatgcc tggcggatga tggggcgaac ctttgccatc caaaagcgct
    11221 acgatgacgc catcgcttgt tgccatacca gtcaatcaat cgcggagcgg agccgagatg
    11281 aactgcgtac aggcggtgcc cgttatgttc tcgctcaatg ctacgaggat ttgggccagc
    11341 tgcagatggc cgttcagtta ctggagcagg tggtgcgaat ggaccgtaag tacgatctcc
    11401 ctaaattagc tgagaatgtg actcgcctcg aacggcttcg cgcccgtcta gccgccgaag
    11461 atccgactcc ccagcctcgg gagtcacgcg catgaatgcc gtcgcgcttc catcatggga
    11521 acaggctcga gcacggctgg tgagcacggt tccgcaattt tatgagctgg agcccggtgg
    11581 agccttggcg cttgatctgg gggatgatgg gtggctgttg gaggtgcggt cggacgggca
    11641 actcctctgc cagcacggca tcgccatgga cgatgtgaag agtctgatgt gcgatgggac
    11701 aaccgaagac ctgggtaccg atgaagtggc gaaacaggcc aagtattttc tgggccctgc
    11761 ggtgtctaag aagcgtcctg cgcttctgaa ggcaggcttc gcggaacaga ccgatatgaa
    11821 tgacgaatat gtcgccatta cgttccacaa gacagtagac tttcagcggt ttgatgaggt
    11881 ccttgctgcc gtgcgctggt gccagaatct attcaagatt cagccatagc acgagataat
    11941 tcagccgggt tgcctcgtga tgcgccctcc catcaccagt attgaaacct tcatcgcctc
    12001 ctggctgcct gccgattgcc gcgcgtgatt gtcaccgcgc tggagctgag tctctttacc
    12061 gcaatgggat cggggagctg gtctatccag aagcttgctc aggctcttga tgtgagcgag
    12121 cgaggggtgg acattctttg tcggaatctt gcgatgtgcg gattgttgag gaagcgcggg
    12181 aaggcctacc gtaacacccc attttccgca acggtgttga atgcgaagca tcccaaatac
    12241 cgaggagcct acgttgagtt gttgcagagt cactgggtgg attggtcgcg gctcacgcgg
    12301 gttgttcgaa ccgggagtcc gctggaacgc gatcagccgg acttgctggc gtatcggcgt
    12361 cggtttacct gggcgatgca tcaccgctcg ttggatgtgg cggcaagagt cgctgcgcaa
    12421 ctcgacttgg ggcaggccga aacattgctg gatcttggcg gaggccctgg cacctacgca
    12481 atggcttttc tcgctcgttc cccaagaatg aaggcaacac tttgtgatcg cgcgaccgcg
    12541 ttggatgtgg cgaaagagat tgctgcgaca cacagggcaa ggaagcggct gtcctatctg
    12601 ccgtccaact ttatggatga agcgctagcc gggtcagtat ggtgtagtgt ggtattccaa
    12661 tgtgctccat atctattccg cggaagaaaa ccaggcgctg tttcggcgat tgtattctgc
    12721 actatccccc ggcggacgac tgatcattca ggatgcattt cttcacgacc gagaaggcat
    12781 ctatcccgaa gaagccagcc tgttcgccgt gagtatgctg ttggttacac ctgcgggaaa
    12841 tacctattca ttcagtgaaa cagccgagtg gtttcgagcg gcgaggtttg tgcggatcag
    12901 gccgcttagg atgaagaagg ggactgaaga ttgggatggg gggtcgttgg aggcgattcg
    12961 tccggccgga aggaaagcgc gcaccggccc ccgaagatca tgatgagaaa attaagcagg
    13021 ctgcttccga gtgtcagtgc caggatcgga gggcgggggg aagcctggct tgcttcctgg
    13081 acaattgtag agagatcgag tgccagggca atcgtgctgt acatgaccgc tgccatcacg
    13141 acccagtgtt gacggagtag gagaattccc gccaccagta gtgtcggaac cccgaacaga
    13201 aaccctaaca aaataccgtc ccggatcgtg gccagcgagt tggctgatcc tgcttcaaaa
    13261 agaaggcctt ccagcacgac cagagcaacg agcagagcga tgaccggacg aaatgccttc
    13321 atggaggcac tataactgcc gcgattgccc atcgcaagtt ccctggcccc tggttctaat
    13381 gccaggcgat ttgtcggggg gctcgttacc agggatcccc ctgtccccgc aagtccaagc
    13441 ttacctgagg atgctcatgt acccaacctt tgaggggcca gcccggcccg ctgtgtgatt
    13501 tacggcctgt gggtattcga gtgagaatcc gcctctcaca gggaggcaca cgtggcccta
    13561 catatatttt cagagagcat tgctgggtgg cttcttggaa cgcgatcaag cagagggtcg
    13621 agaaggttcg gtcgtcaacc aaaggagcga cggttggcgt tagctttgtg ggcgacgagt
    13681 gtgcttgtgc tcatttccgg cctttcctca gctgccctct cccatgtatc tcccggcagc
    13741 attaccgctc ctggatttag tgggaagggc gtggcccaag tgcctgccga tcattcagaa
    13801 gtgttagccg ccgggtttgg gtttcaggcg gccgggtcgt caatgattac ggtacggact
    13861 tacgatgcgc cgacaggcgc catcctgtcg gaggattcgt tcgatgtaaa cgtgaaggaa
    13921 gagggcgtcg ccgaacatga tgggaataag gggcgcatct ttgccggtgg cattggaact
    13981 gactctaaag ggaagtcagt ctttatgttg agagtgtacg atgcagaaac ggggcggttt
    14041 ctttgggaag ggcagctgaa cttgctcaaa ttgggcgaag gagggatgac gagagccagc
    14101 gcgacgatca tcccgacccg cacatcggcg ctacaagtat cggtttccag caccactcca
    14161 cttcagaccc tcttctcggt tcgggccgta aatcctttca cgggcggact tgtctggcag
    14221 gatcagttcg ccccaggggt tcgaaagcgg gcccgggtcg aaggggtgtt attcggtggg
    14281 ctgttcgtca ggccggtcgg cgaccccatc ggacatattt ttgatttggt tgtgcggacc
    14341 tacgatcgca cgtccggaac gctgttatgg gaggattcgt tcgagcaact ggatcgcatt
    14401 gaggagtctc cgaccgagcc ggagcttcgt gcccatcccc aggccattcc atcttggaat
    14461 cttagcgctg cgtcgaggca tcacgcgtac cagacgaagc tgcgataggc ttaggatttt
    14521 ggctgagcca gtgtggagcc cagactgtcc gcccattgct gctggaagtg atcgtatgac
    14581 agcagcagcc tatcctgcat ggcggcagca attgtctgac gagccttcag gtggaccagc
    14641 acctcgtgaa ccttgtgcat gccgaaccgt tcaatgagat agtgggtggc ggcattggcc
    14701 tccctataag cgagactgac cttctccgca ggtagactct cccaactacc ctcaagcgcc
    14761 gtcaaaggaa ccaggctgtg ctcaccgggc gtgacgctgg tcacctcttg ccagggatca
    14821 ccggccagct gcattgccaa gccttcattc agccaggttg gaatcattcc accggttgcc
    14881 cccaattcct cgtgaatcag ggcatgcaca aattcgtgac gaagcactcg agtcagccaa
    14941 gcccgatccg ttgcagcgcc ttgggttggg atttgaatgc gcccaaggac gggatcgaac
    15001 aggccatccg cccaaccggg gcttcccgtg gcgccttgaa actgattctt tgtgtgcagg
    15061 acgactatga tcggctttga cgggaaaaag ttgaatttct gaccgatttc ccgataggcc
    15121 tcttcaagaa tatccagcac ggtcgtccat gtgtccgggt cgtcacctcc gtcgtacttg
    15181 acggtgaagt ggacgctgct gtgcgtcgag aatttatcct cgaccttctc ggcgcgatgt
    15241 actttcgtcg tgaccgcctt cagataagac tgaaggccgg ggtctttctt aattcgttcc
    15301 ttggcctgcg cgaggtgttt gccggcttcc gggaggtgat ccttctcctg tagcaggtct
    15361 gccaaggcaa catgggggaa cggctcatcc ggagacagtg ccacgacttt ccggaggaaa
    15421 tcttccgtga gtgcggggtc ccgcaacccc cagtaggcat gggcgagatt gaggtgggca
    15481 atcggatttt gggggtcgag cgcgaccgct ttcttaaatg ccttcacgga aacttcggtc
    15541 cccccgcttc tttcctggat gattccgagg ttattccaca agatggcaac atgccgcttg
    15601 gtggcggatt ccgtcaggat cgacgacggc agttcctcca gtttcgtctc tgctaaggca
    15661 atattgtgct tctcgagctc ttcgtgaatg gctccgagca gctcgggatg tctcggaact
    15721 ggaatgctga cgggatcgat agtcctcgac cgtttgatct ccggggcggt acgttcctgc
    15781 gatggggcca caggagcgtt cgacgcaggg gtggtttcct cgacaatgac ctgcggtgat
    15841 ggagccggtg tgtcgtggaa atagcgaggc ttcagccagg tttgatagaa aataaagatg
    15901 ccgatcaaca cggctagagg cccgagaata tgtttgatat tgcgtcgata cataggtgtc
    15961 cttccgtgaa tcctacgcga attcactgta ccaccgagcc gggagcggac aaagggaagt
    16021 tgaaaaagca gccggggtct gtgcgactgg ctaccattcg gcagaagtcc cctcgttgag
    16081 ccgtcaaggt ggctgtggta cgatcctgct ccaggatgcc acgtcctgaa tccgccgact
    16141 ctcacgatcc gttccaagcc ttcctgcatc gtgtaaccag accgattgaa tttgcttgtc
    16201 gcgatgccta cgcccatctc tcggcggtca ggaatcttga ccgttttgtg tcgcaacagg
    16261 tgattggcac tcttggcgag cgagtctatc cgcgagcgct ggagacggaa ctgatcgcac
    16321 tacgcaacct gtttgtcgac tttcacactc ggctcacacc cctcgaacag caggatcggc
    16381 tcacgaaagc gctggcgctg ctcagccgcc tacaagggga cgcgcgagcg gtgagtcagg
    16441 cggcggggtc tccgaaggag aaccaagtac aacctccacc agttcggtcc agtcctgcca
    16501 gaccgctgtg ggagctatcg attcaatatg caaaaggagt tggtcccaaa cgaactctct
    16561 tgctggagag gctgggcgcg cacactgtcg agcaggcctt gtggacgtta ccatggcgct
    16621 atgaagatcg ttcggtcatc acgccggtgg ccgagcttgt gccgggggct acacggagcg
    16681 tttgcggcgt gatcactcgg gcagaggcaa cgcgtgcgcg cgttcgccgg ctgtcgattc
    16741 tcgatgtggc ggttcaggac gcaacgggga ctgtgcacgc ggtgtttttt aatcaaccat
    16801 acttggaaga tgtgttgaag gaaggcctgc gggtcatgat gtccgggcgg gtggcggccg
    16861 gtcggggtgg gtggaccgat gtgcggttgg aagcgacgca atttgaagtc ttgagcggcg
    16921 gcgaggatga gttactccat gtggggcgca tcgttccgat ctaccatgag acgaaggggt
    16981 ggacgtcacg tcagatgcga gttttgatgc aggggttgct ggcggagtat ggggctggca
    17041 cggaggaggt actgcctctg tctgtccgtg cgcgccatcg gctgccgccg atcggcgagg
    17101 cgattcaaca cgtgcatttt cccttgccga agacggatct ggccgccctg gatcaggggg
    17161 tgacgtctgc tcaccgacga ttggcgtttg aagaactgtt tctgctacag gcggccatgg
    17221 tgcttcgcca acgggagatg aaggaagagc tcaaatcatt tcggtttaat cctcacgtgg
    17281 cgcaactgaa gcaactggcg aagatccttc cctttacgct gacctccgcg caggaacgtg
    17341 tgtttcgtga gatccaagca gacatggtga cctcgcggcc gatgaatcgc ctggtgcagg
    17401 gtgatgtcgg gtccgggaag accgtcgtgg cgctgcatgc ccttgtgatg gcttgtggat
    17461 cgggctgcca gacggcgttg atggtcccta ccgaaattct ggccgaacag cactatctga
    17521 atcttgcccc cttgctgagg gcagtggggt tgaaggcagt gcttctgacc agcggcggga
    17581 aggcaaaaga ccggaacgag acgctgaagc agttggagtc cggcgctgcg cagatcgcga
    17641 tcggcaccca tgcattgatt caaaagaagg tgcggttcgc taaccttggc ctggtgattg
    17701 tcgatgaaca gcataagttc ggcgtcctgc aaaggaagac gttgctggag aaagggtatc
    17761 gaccggacgt gctggtgatg acggcgacgc cgataccgag aaccctggcc atgacggtct
    17821 atggggattt agatgtatcg gtgatcgaca tgctgccgcc gggccgcaaa ccggtgcgaa
    17881 cattgctcta taccgacggg caacgtcaca agacctggca gctggtgggg gaggagttga
    17941 aggccgggcg ccaggcgtat atcgtctacc cgctggtcga ggaatctgag aaggtcgatc
    18001 tcaaggcggc gatccaggga gcggaacacc tgcagcgcga ggtctttcct caggcgcggg
    18061 ttggattgct gcacggccgg cttccgacgg ctgaaaaaga gcgcaccatg gcggccttca
    18121 aggccgggac gattcaggtt ctcgtcgcga ccacagtgat cgaggtcggg gtagacgtgc
    18181 cgaatgcgac ggtcatggtc atcgagcatg ccgaacgatt cgggttggcg cagttacatc
    18241 aattgcgagg gcgtgttggg cgtggggcac atcaatcgct ctgtattttg atggccacat
    18301 acctcgcgcg agaggccagg acgcgggtca gccgtgacgg ccggccggag cagaatgcct
    18361 ccaatgcgca acaacggttg gccgcgctcg tgaactcgaa cgatggattt gtcattgccg
    18421 aagaagatct ccgaatcaga ggccccggtg aatttctggg gctgcgtcag tggggggtgc
    18481 cggagtttcg agctgcgaat ctggtgcggg acgcgcaatt gttggagcag gccaggcagg
    18541 aagccgtgac gttgttggaa gaggatccgg gcctgatgtt gcctcagcac caggcgttca
    18601 aggccgcgat gtttcgtcgc tggcggggta ggctggcctt gggagatgtg agctaagcag
    18661 ctgggacact gccggttggg tggagggtga tcgatgggaa tgttgcagcg cttgagtcac
    18721 gatttgcggg ccggctgggt gacattgcga cacggcacgg ccaaggccgc cacgcgcgcg
    18781 ctggaagagg gcgaactact gcgctaccgt ctggaattgc gaaaggtgga tcaacagctt
    18841 gacgatctcc acggggacat cggcgaacgg acgattgcgt tgcacgatcg tggtgaatcg
    18901 gcagaccgta tcctgtccga ttctgaaatc acccgcctga tgcaacaggt gcggacactg
    18961 caagatgaac ataccaagtt gctgctggaa atgaacgaca tcacggtcga aggccaatga
    19021 gactcagttg atgatccccg agacgactgc tcccacacga attttcgccg gtatcgatat
    19081 cgggacgctc acttgtcgtt tgctcatagc ggaactgacc tctgccggag ggttgagaga
    19141 actccgatct gaccgacgca ttctccggct cggtcaaggg gtggatcggg agcgcgtgct
    19201 gcgttccgat gccatggcac gagtggtggc cacgttgaaa gaatggcgtc aggtcatcga
    19261 cggctatcat gttgacgcgg caacggccgt ggcgacgagt gcggtgcgcg atgccgggaa
    19321 tcgtgatgag ttcctaggcc tggtccaacg ggaagccggg ttccaggttg agatcatttc
    19381 cggcgaggag gaagcccgcc gaaccatgct tggtatccgt tccggcctgc cctctggtgt
    19441 gacggacctg ctggcgttgg atatcggggg agggagcacc gagtttattc tggaccggcc
    19501 cggccaggcg acgctggcga gatccatcga tatcggtgtc gtgcggttga gcgaacggat
    19561 actgcacagt gatccgccga ccccagacga ggtgcaggaa gcgcgcgact gggtgcgccg
    19621 ggaaaccact gccgcaatag cagcaatgcc acggtcgccg ggagtcatgt tcgtcggcac
    19681 cgcaggaacc atcactgcgc tggccgcgat ggttcagcag ttgcctgctt atgagccggc
    19741 aagaattcac aactacacgc taacgctaaa tatggtgaag cagttggagt tgcagctact
    19801 cagttgttcg aaggcgcaac gggaaggcct gccggggctt gaacgtggac gggaagacgt
    19861 cattgctgcc ggtgcggtga tcctaggtac ggtaatggac acgttgggaa tgacgaaagt
    19921 gctcgtgagt gacctggggc tgcgggaggg tgtgctgata catcttgccg gtggtcgata
    19981 agtgctattt ttttcagtca gtcgatttat ttttgagcgc gtgctgatca atgacaatcc
    20041 tggcttctgt tcagttgaag gctcagttaa agcgtaaggt ttccctctgt cggatcgcga
    20101 tcacacttga gtggccccta gtgctgaaat tgaggaatcg ccagaacgtt accgctgcga
    20161 ataccagcgg aggccgcttt tctgctcggt gaaggtcggt tgggggatgc cgcccggctt
    20221 ttccaggagt agcaccttga agtcgatgag gccgaaccag gcggggtcgg cgatgtcgtg
    20281 gtagtggcag ccttggagct tggggaggat gtcgcccagg gttttggtga gctcactttc
    20341 cgtgtaggcc cgcacagaaa agttctgccc cagtccctga cagaatcgtt gaatcgtata
    20401 ggccgcgccg gtgcagagga ctttcgtgtc cggtttgatc tgcaaaaact gcgggagtga
    20461 caggtaccgc tcctgaaagc caagccaacg taccgcttgt cgcaggtggt tgtactgcac
    20521 ctcgtattcc gtgtggtggg ggagcttcac ggcggcgggc caaccttctt cgacgccaaa
    20581 ctccgtgaga aatgctcgac cgcccggctt gagtacccgc cagacttcgg ccacgaaccg
    20641 catcggtccc agattgaaga tcacttcttc gggcaagtcc ttttccagcg ggagacggag
    20701 ccgtctgatc cagtccaagg cttcttgatg ttgtgcggtc tcgcctgtgc cttccgtgag
    20761 ctccttgcgg ctgagcttca ccggcgtcat gtcggccata ttttcgttgt cgatgatgag
    20821 gtcgacggag ttgtctctaa agggcaggca ttcaccgtta gcatgggtgc cggtgccggt
    20881 ccagccgccg gccttcgcgc gtttggtctg gagctgcagg aacgggcgcg tgacatccaa
    20941 cgaaatgtat tgaatgcctt gtttctcgaa cggcagtagt tcctgtccta gctcgcgggc
    21001 gacataaccc aatccaccac cgatctcgac aatgactttt ggcttcggtt tgaaccagcc
    21061 gagccggcgt agctggcgca tgagcaggcg gccataagtc gtgccgccca gggcttcgca
    21121 gggttcccgg aaaaggtggg agacggtggt ttcgatcaga tcgaagtggc catcctccag
    21181 gtcactttcc ttcaactcgt ggacatggaa gtcttcgagg tgttcctcac cttcgaactc
    21241 ctgctgcccc gaccagcctt cccggatttg ttgcaggagc aggtcccatt tggccttgtg
    21301 gcgatggccc ccgggcggct ccgtaccgaa gtagcacagc gaataattgg gcgtggacca
    21361 ccgtgccagg tgccaggttc cgggctggcc gtccgggccg cagatcttat taccgtactt
    21421 ctccaagagc gcgcccaccg taatgtggcc gttcatcgag gtgagcattt caatcccgac
    21481 ggcattgagc cggacgagcg ggaagtcgtc gtcgagcggc gccagccacc attcgtcgaa
    21541 agcgtgctga taggctacga ggtcgggaat acgccaggca agcaggctga gggtatcgcc
    21601 gctcaatgtg aggggctctt ggatggctgc ggcagctttc atctggttag gtcctaggca
    21661 cgaaaagccg cagcctacac gagcgcggcg ctgacgtgca agaacctcga tgaggaggag
    21721 gggctacgtc tgcgagggaa gcagcggcga ctccgtcacg ataatcatgc cgcgcataat
    21781 ggggtgaatg cggcattgat aggggtaacg gccaggcggc aggcttggga tggtgtaggc
    21841 tcctcccggt tcgacggccc ctgagtcgaa ggcgcaacgg ccgctttctt caaggcatgc
    21901 atcgtgggtc acggtatggt gggtgggcgt tggattttcc caacggatgg cgccgccggc
    21961 ggttacggtc acggctgcag gcacataata gggtgatccg ctgtccatca ggacttgcgt
    22021 cgccggtggc gtcgcagagg tcggccaggc tatcaaggtg cttgtcgcca atacgaagca
    22081 gaacagtcgc cagctcatag cgtcgtcatt ccggtgaatc aatatacatc tattatacat
    22141 agagacaggt tcccgacgga aggattcagt cggcctatgg ggaggcatcc gcttgggctg
    22201 gaggacggaa caggccgggt cgtgctggat gcgcccgggc ggcgccgacg gcacgaatgt
    22261 gaatgcttgc aggggcgcga tagcgttgca gtctgacaca gtcagccgtg ggtgcaggat
    22321 ggttgcaaga acccggttgt tcatgctaga tggaggccgc tttaccaccg acgtgccatg
    22381 ctgaagacct gagaggagga cgtggaatgg cgaagaaaat cgtgaaaaag aaaagcgttg
    22441 ctgccaaagc ctcgcgaccg aaaaaagtcg cggcgaaaaa ggcgacgaag ccgaagaaag
    22501 ccgtggcggc taagaagcct gtgaaggcca agaaggcggt gaaggggaca cccgctgcca
    22561 agaaagccgt caagccggca aaaaaaatga ccaaaaagcc ggccgcagcg gctaagcgtc
    22621 cctctaaggc caagcccgtc tccaaagcgg tgaagccggc gccgaaagcc gtcaagcctg
    22681 cggcaaagcc caaacccaag cccgcgcccc ccaaggtcaa acctccggtc gagcagtcgg
    22741 ccgtgattcc tgagtcgaaa ccggcgaagc ctccagtgcc ggaagtcagc aaggcggcaa
    22801 aggtggagcc cgagcctcaa ccgctggagc tcgacgacga agaggaaatg gcggaagacc
    22861 ttgaggatgt gtcggaagaa atcgaagaag agatcgaaga agaactcgga ctcgatggtg
    22921 aagacgacga cgtggactat ctggaaaagt cggaagatct cctcgacgac gcagacgagt
    22981 atcgcaacaa ttagcccacg ttggtgtcgt ggcttaccgg gttgtgctcg gttgatgcct
    23041 ctccgtgtca catgctccct agccagtgaa ggctccactg aacaggcctg atggtgtctt
    23101 ccgtcccgac aggtctgaga ctgtctcggg gagactcctc gagccagttg ctgtggtgct
    23161 atgctggtcg cggcttctgg ccatcattct gatcctataa ataaatttca tgagccattt
    23221 tttgtttatc gccggcgccc ttcgtgaacg aacttcccag gagagtgccg agctgcagtt
    23281 tggcgccggg atttggggtc tctgcacggc cttgattcgc gacaatctcc agaagttttt
    23341 ggtgcctgac tcccacggac tggtgtatgt gctgaaggaa ggcattcgag cggaatttgc
    23401 gatttcttca ccggtccagc cgtttcaagc cctggatgaa ttgctcaagg acgaggtgcg
    23461 aacagaagcc cgatatggat tcgttcggat cacgccgctt cggcgttggc aggttcatgc
    23521 gccggactgt caggccctgc ttcagcgggt gttggggatc gaggagcagg cagaattaac
    23581 tcggcggctt agtctcggca tgcatcgtct cacggacgcg gagtttcggg caatcgtcga
    23641 agggttgggg gtggaagcgt agtcggcgac gtccaccgac cgcgggactc ctattcgagt
    23701 gtgcctccgg tcatgagacg gtaggcctcc tcgactgtgg cgacctctgt gacttcaacg
    23761 ttgagctccc aggcgagccg gttgaggttc cagcgcggat cgtcccgctg accttggggg
    23821 atcaggattg tacgataacc ctcgcgggct gccgcacgaa tcttgtcggg gatgccgccc
    23881 acgggtccga tggtgccatc cttggcaatc gtaccggtta aggcgatccc tcgccggata
    23941 tggtctcctt tcaatagcgc cgcaaatccg accgtcatca ctgcgccggc gctcgggccg
    24001 tctgtcccgg ttgcggcgta tgcgatgccc atggcactga ccgtgcccgc ctgatcgatc
    24061 tgtggcgtgt ggtcgagcgc atactgaaaa gcaaggacca tggagctgag attggtctgt
    24121 ccgggccgga tgttgccgcc ctgccattgc agctcaatgg gagaggggct tggccggtga
    24181 tcccaacgca acaggagcac ttcgaacaca ccgcggttat tcgcttggat cgctgcgagc
    24241 atcgggactt cgaccaccga ttctgcgcag gcaagccgga tacttgcgag tagcatagcg
    24301 gtgcagacaa agaccgcgca taggaagcgc tggcctctgc tggtgcgaac ctcgggcgca
    24361 atggcggaca tatgagtcga agtgtagcaa aggtattggg ggagtaaagt gttcggacgt
    24421 ttacccgtct gtcgtttggt tcggagaggt tttgtgaatt cattgaaccg acccgctgga
    24481 tcatgtcgat ttcccatctg ggccgcaata gcggtgctgt gtggattgtt gctgctcccc
    24541 gggatgctgc gcgcagaatg tgtcgcggag aggggcgtga aaaacgcagc ggctgtgttc
    24601 accctgcatt taccgaatac ctgtactgag gccgagcggg aggcgcgtgc cgtacccgca
    24661 caggaattgc tgcgggcgct gacggcgggg aaggggttgg acctgtcggg agtcgtgatt
    24721 cagggcgacc tggtcctcga tgagctgccg gctcagaagg cgagtgcggt gggcgacctg
    24781 gcacccgagg accgtcgagt gcttgagggg ctgaatgacg aggaggtgca tgtcatccgc
    24841 gggccattcg tgatcaagca gtcgagggtg aaaggacgga tcgtcaaccg gctcaagagc
    24901 gggtttctgt tgatcaccgg accggtggtg ttggcgcaca cggatttcgc cggattcgtc
    24961 gacctctcgc gcaccgtgtt tctcggcctg gtcgatggat cgaatgcgac cttccatcag
    25021 gagagttatt ttgttcagga tcgatttacg cagggagcca tgttttcgga tacgcatttc
    25081 ggtccgcacg ctcgatttca ccgctcggtg tttgccggcc cggcgatttt tcgcggggcg
    25141 acctttcagg ggttaaccga gtttctggaa gtggtgtttg agcaggatgc aaatttttcg
    25201 cgggcggcat ttcacctcgg aacaggattt tccggtgcgc attgccgtgc gaagtgcgac
    25261 ttctcttcgt ctcagttcga tcgggaagct ttttttctgt tcgccatctt cgaccgtccg
    25321 gttacgtttg cctccgcgcg attcggatcg caggccgact tttccgatgc cgcatttaaa
    25381 caggccgatg atctcgcaca ggcgaccttc gcgcgcacac cgcagctgac cagaaccgct
    25441 cgtgtcagca caacggtgcc gggtcctacg gcttctgcct cggctccatt ttcccaggcc
    25501 gtcacgatcc tcctatttgt catggcgctg gggctgttgg tctatatcat cagggccaag
    25561 tagccctggc tcaacccaca acatatagtt ttagtcttgc ccggtccccc cataaattgg
    25621 tataagattg aaactttggc caagggagcc tctcgtacct gatggaccaa cctgaactgc
    25681 cctaccaagt caagatcgag aatttcgaag gtcctctcga tctcctcttg cacctcatca
    25741 agaaaaacga aatcaacatc tacgatattc cgattgcgct gatcgcgcag caatacttgg
    25801 actatctgtc ggtgatgaag gaattgaacc tggctgtggc cggtgaattc ctcgtgatgg
    25861 cggcgaccct gctgcatatc aagtcacgga tgttgttgcc ggtcgacgag gtggcggtgg
    25921 acgaggagga cggccccgat ccgcgcgaag aactcgtccg gcgactgctc gaatataagc
    25981 agttcaaaga ggcggcttcg cagctggact ataaagaacg tctatggcgg gatgtgtttt
    26041 cgcgcgagcc cggtccgccg gtcgaggtca agaaagacga gagtctgctc gacgagattt
    26101 ccctctttga tctggttgat gcgctgcagg gggtcttgtc ccgccatcca ggcaagcgac
    26161 ttgttgaaat cattccggac aacctcacgg tccgcacccg tatgagcgcc attattgagg
    26221 cgctggaact gcatgaatcc atggccttta cggcgctgtt cgaggagtcg agtcatcggc
    26281 ttgtggtgat tgtgacgttc ctggccctgc tcgaactgat tcgaatcagg gtcgtgcgcg
    26341 ttttccagag cgaaaccttt ggagcgattc ttgtctcgcg tgccttttcg gcggttgcag
    26401 aaggggatct agtggaggag cctgaatgga agaacctatg agtcccggcg tggaggaggc
    26461 aatcgaggaa tcgatcgagg tgcgggaccc ggttgacgag ccgactgcct cactcgatgc
    26521 tgtcgaggtg gagcccgagg ttggccaaga cgttatcact gaaatgtcgg gcccggcggc
    26581 cgatcctgcg ctttccgata tgcgagccct gaagggcatc cttgaggcct tgctctttgt
    26641 gacggccgag cctattccgg tcacgcggtt cttggcattg ctcggagcgg ttacgaagca
    26701 ggatgtcgat caggcgctcg cgagtcttgc tcatgactat gagcaggaag gccgcgggtt
    26761 gcagttggct gaagtggccg gcggttaccg aatcgtcacc aaggccgaat tcgccccgtg
    26821 gctcaaacgg ctggaaaagg taaagtctcc ctcaaaactc tcgcgctcag ccttggaatc
    26881 cctggcgatc attgcctata agcagccgat cgttcgcgcg gaggtagagc agattcgagg
    26941 ggtcgaaaca tcaggggtca ttcggacgct gctggagcga aaactcgtgc gcatcgttgg
    27001 ccggaaggaa gagcctggac gcccgatcat gtatgggacg accaagtttt tcctcgaaca
    27061 ttttgggctg cgcgatttat ctcagttgcc gccgcttcgt gagttcaaag agttgggaga
    27121 atccgagcag gcgatgttgc cgattgatga tgtggtggca gtggagggag agccgctgct
    27181 gcaagtcact gaggcatcgg gcgaaggcct gtccgaagac atggctgagc ctgtggcgct
    27241 tcttgagccg ggagatgaag cggggcagac ttcggagtta cttgttctgg agaacgaggc
    27301 cggaggggac cttgtagtcg ccgatatcgc ggaagagtcc tgactgctgt tcgcaatgtc
    27361 tgctcaaaga ctccgatagt attcgccccg cattgccgcc cgcgcttttt cagtcgttat
    27421 gcataaaagg ccttcgagag actcctgtga agggggtttt cccgaaggcc ttttgtgtct
    27481 gacgatcagc agcagttcgc ggctgcgagc tcctagtggc aaccgattgg aacaaaaccc
    27541 gcgccgaaga tctgtgacag ggtgacgtat cgggcattgt catagaatga gtaactcggg
    27601 gcgtaccagt tacgggccgt tggattactg ccgtacgacg gatcggtgcc gaagaagtgc
    27661 ccgccccggc tgttctggtt cagatgaatc cattccagat acatgctgca aacttcgcct
    27721 tgcaccgacc cgccgctagc attgcccgca taccagtcgc gtcctgactc gggtatctgg
    27781 gagagattgc gcaaggcgcc gatgggagtg tcgtatgaaa aatctttcgc gttcggggtc
    27841 ggctgatagg ttgaaccgct gggaggggcg agtggcagat cgggcagcga gggcacaacg
    27901 gagagcgctt tgaggacctt ggcctgacaa tcgggtcgct ctttattggt gtagaggatc
    27961 gttccctctg ggcctgggca ttcccacatt tctacagggg atgactcgac cgcgtgcacg
    28021 ctgggagtaa ggagcagact cgcggcgagg gttgcgagca caagtcgtgt tggccacatg
    28081 gtacacctcc gattggttca tcttaccggg cacgcgcgtt gttatccaac gtcgagagtg
    28141 cagagcctat gacattcgaa agaaatgcgt ccatccagct ttggggggat ggatgcctac
    28201 cctgcccctc gtcctagcgc gcggcttcgt ccgctctcgt cgacgcggtg actccattgc
    28261 cgatgtatca gcggaagagc ctccagcgcg ccccaggtca cgcgggtctt gccttgactc
    28321 ccttcttttc gctttgttag actgcccgat ctaagttatt gaaacatccg ccaatcttca
    28381 ccaatctcgt accacgagga acaccatgat caaggcttgg ctgctcgacg aaatgtttcg
    28441 gtttgaccgg cggtatctca cggcggaggg aagtcagagt gagtggggcg ccggggactc
    28501 cgttgctgag gaggaagacc tgcctcctgc accgactggg gtggccgctc aagctggaaa
    28561 tggccgggtg atgattacct gggacgctgt gccggaggcc atgtattaca acctgtattt
    28621 catgaccacc aagggcgtac agattaaatt ctccgaactc actcgcccca tcgccagcgc
    28681 ggaagatttc aaaaccgtca tcggcgtgac caaggaaaag gggacatgta ttgaaggtgc
    28741 gcagtcgccc ttcatgcatg acgatttggc aaacggaacg tgctaccact atgtcgtgac
    28801 cgtggtgacg cagaaggggg agagccccga atcccaggaa gtcatggcga tcccggctcc
    28861 ctacctcatt gccaagatca tcgggcagga gggcgtggag gacggcgagt tcagttctcc
    28921 gactggtatt gcggtagaca agggcggcaa tatctacgtg gccgataccg ataatcactc
    28981 catccagaaa ttcgacaaag acggaaaatt tctcggtcgc tggggcggcg aagccggcag
    29041 cgcagaaggc ggcttctatt atccgcgcgg actgaccacg aattcggacg ggcaggtgta
    29101 cgtcgcggat accggcaaca atcgcgtgca gcggctcgac accgacggta atcccatgaa
    29161 ggcctggggt aagttcgggt ttgcgtggcg tggggccgac atgaataagt tcgacgctcc
    29221 ctggggtgtg accaccgatc aggacggcaa tatttacgtg accgatacca acaacgcccg
    29281 cattcaaaag ttcaagggtg atggaacccc gttgttgaag tggggtcggg atggaagttt
    29341 cgacggggcc tttttcttcc cgcgcggtgt ggccgtggac ttcgtcggga atacgtatgt
    29401 ggccgatgaa gggaataatc gcattcagaa gttcgatacc cgcggaagct ttttgaccaa
    29461 gtggggccgt gaaggaagcg gtcccggcca gttcaaagcc ccctggggcg tgacctgcga
    29521 tgccttgggc aatgtgtacg tcgtcgatca gggcaaccac cgtattcaaa agttcgacgg
    29581 aaacgggacc ttcctctgtg cctggggaaa tcgcgggaag accgaaggcc agttgaactt
    29641 cccgtccggc gtggccgtgg acaaggaagg ggcggtatac gtcgtcgata gcggtaacca
    29701 tcgggtcatc aaatacgtgc ctaccgatga agagctgaac cgcggcaagg cggaacgcga
    29761 acgggcgcaa gccgtcagtg agtacccgat tccccgcaat ctggcgatca agcccggaga
    29821 taccgaaaca ttcttgagct ggatggatgt acctggggcg gtctcctaca atctctactt
    29881 ccatacctcg gcgaatctga cgcaggaagg cggcactaag atcgagggtg tgaccagccc
    29941 atacaatcac tcgggattga ccaacgacca ggcctattat tatgcgctga cggcggtcta
    30001 tgaggacggc acggagagtg gattgtcgga cgaagtgtcc gcgacgccgg tgctgatcga
    30061 tatcacggct ccgcagacgc cgtatgcggt gatcaaccac ggcgcgttta tgacgaactc
    30121 gcctgaaatt gttgtcacga tttccgccac cgacctcgat accggcgtgg cggcgtacta
    30181 catctcagag aacccgatga ccccgatggc gggaaccccg ggttgggtgg aggttccgcc
    30241 ggcgatcaag ttcggcgcga cgattccctt cattctgtct cctggggacg ggcagaagac
    30301 cgtcatcgtc tggttcaaag acctcggaaa caatatctcc acgccggcga gcgcgacgat
    30361 tctcgtcaac acctccggct atctttgtgt ctccaagtgg ggcaagcctg gtcgaggcgc
    30421 gtcgttgttg cacggcggtg aattcatggc gccgatgtac gggctggcca tcgatcagca
    30481 ggggtcgatc tttgtcgtgg acaacgggaa caaccgtatc cagaaattcg atcgcacggg
    30541 gaatttcatc attctctggg ggaatttcgg cgccgccaat gcgaacttcc ataacccgac
    30601 gggtattgcc tgcgacgcca agggcgatgt ctacgtggtg gataccaaca atcaccgcgt
    30661 acagaagttc gacgggaagc tgggcggcta tttgatgaag ttcggatcgc gagggaacgg
    30721 cgaaggccag ttcaatgccc catggggtat cgcgatcgat cgggtgcgcg gatacatcta
    30781 tgtcgtggat agcgccaact tcagagtaca gaagttcgac atgagcggag agttcatcat
    30841 gtcatggggc agtttcggca acggcgacgg tcaattctat ttccctcgcg ggatcgccgt
    30901 agatcaggcc gacggcacgg tgtacgtcgt cgatatgggc aatcaccgcg tgcagaagtt
    30961 cgataccagc accaacgtat tgccgcagtt gctcaccaag tggggcggca gttccgaggc
    31021 cggccatgcc agcagcccgt tggcgcagga agccggacag ttgcgctctc cttggggcat
    31081 tgccgtcgat ggtcagggcg atgtgtacgt cacggatacc ggcaaccatc gtgtcgagaa
    31141 gttcgaccgt gagggcaact ttatcgctca gtggggcgga ttcggcggtg gtgacggaca
    31201 attcaacttt ccgtacggca tcgtggtgga ttcccgtggg agtgtgttcg ccgttgatag
    31261 cggaaacacc cgggtgcagc aattcatgcc ggcggatgaa ggcagcgagc ggttgcagga
    31321 agaggcggag gccgccgcag agttgggaca ggtcgataaa accacgagag tgtaggggca
    31381 acgggcgaga ggcccgggag agggtggcga ctgatgttag agaaggagat tcggctcggg
    31441 ctcacctatg acgacgtcgt cttggtaccg gctaaatcac aggtcctccc cagcgaggtc
    31501 gatacccgta ctcgcctgtc tcgaaacatt cagctgaata ttcccatcgt cagcgcggcc
    31561 atggacaccg taaccgaggc gcgactggcc attgcgatgg cgcaagaggg tggaatcggc
    31621 atcgtgcacc gtgtgctttc tcctaccgat caggcggctg aaatcgataa ggtgaagaaa
    31681 tctgagagcg ggatgattct ggatcctatt acgatctcgc cggatcagac catccgtgat
    31741 gcgcatgacc tcatggccag ataccggatt tccggtatcc ctgtgaccaa ggcgggtaaa
    31801 ttggtcggga tcctcacgaa tcgagacttg cgttttgaga cgcgaatgga actgaaggtg
    31861 tcgcaggtga tgaagcgcga taagctcatc actgccccgg agggaaccag cctcgaaaag
    31921 gcccgtgaga ttctgcatga gcatcggatt gaaaagctgc cggtcgtcaa caagcaattt
    31981 gaactcaaag gcctgatcac gattaaggac attgagaaac ggatcaagta tcccaatgcc
    32041 tgcaaagatg cccacggtcg tttacgggtc ggggcggctt tgggggtggg acccgatacc
    32101 gcagaccggg tggcgttact ggtgaaggcc ggtgtggacg tggtggtcgt ggatacggcg
    32161 catgggcatt cgcaggctgt gctggatacg gtcaaaatgg tgagaaaagc acatcccaaa
    32221 ttggacatca ttgccggcaa cattgcgacg gcccaggcgg ccaaagattt ggtcaaggcc
    32281 ggtgtggatg cggtgaaagt aggcgtagga cctggttcga tctgcacgac acgcatggtg
    32341 tccggcgcag gcatgccgca gttgacggcg attgcagatt gcgccagggc attggccgga
    32401 tcgggtgttc cggtcatcgc cgatgggggg attaagtact ccggtgatat tacgaaagcc
    32461 ttggcggccg gtgcctcggt ggtcatgctg ggcagtctgc tggcaggaac ggaagaggct
    32521 cccggcgaaa cggtgctgtt ccaggcacgt acctacaaag tgtaccgcgg catgggttcg
    32581 atcggggcca tggaacgagg tggcggcgat cgatacggcc agggcggacg accgacacca
    32641 aagctggttc ctgaaggcat tgagggccga gtgccctata aaggcacgct tgctccgcat
    32701 atctaccaga tggtgggcgg cgtgaagtcc ggcatgggct actgcggatg taagaccatc
    32761 ccggatttgc agcagaaggc cacgttcatc cgccagacag tggccggcct ccgcgagggg
    32821 cacgtgcacg acgtcatcat caccaaagaa gctcccaact atcgcaccga ttgggaatag
    32881 cctcgcgtga aacgtttctc gtgaagcgta tctcgcaatt cagatcccct cactagacga
    32941 acgacgattc acgcttcacg gaaacatctc catggaactc tggcacgata gaatccttgt
    33001 ccttgatttc ggatcccagt acacgcagct gatcgcacgg cgtatccgtg aagcgcaggt
    33061 ctattcgcag atcctgccct gcaccgtgcc gctcgcgacg gtattagcct accgtccgaa
    33121 gggtatcgtg ttatccggcg gaccatcgag cgtgtacgac aaaaaggctc cttcggtctc
    33181 caaacaactc ttcgaacagg gcattcccat tctcgggatt tgctacggaa tgcagctggt
    33241 gacgcatctg cagggcggcg aagtggagaa ggctccgcac cgcgagtttg gccgggctga
    33301 gctgaccctc gatgaccggt cggatctgtt caagggaata gggactcggg gctctaccgt
    33361 cgtgtggatg tcgcacgggg atcgcatcga acggatgccg ccaggattcc gctccatcgc
    33421 acacacggac aattctccca tcgccgcgat gaagcggcac gacgccaggc agcgcatcta
    33481 ctgtcttcag tttcatccgg aagtggcgca caccaccgag ggcgccacga tcctcaagaa
    33541 cttcgtctac gatatctgcg gctgcaagcc gacctggacc atgcgctcct atgttgacac
    33601 ggccgtggaa cagatcaggc aacaggtcgg gacggagcgg gtgatctgcg ccttgagcgg
    33661 cggggtggat tcctctgtgg cagcggccct tacgcaccgt gcggtcggcg atcaactgac
    33721 ctgcattttc gtcgacaacg gggtgttgcg taccggtgaa cgtgaccagg tcgagaaaac
    33781 cttcgcgtcg cagatgcatc tgaacatgcg gatcctcgat cgcaccagtc agtttctcac
    33841 gggcctgaag agcgtgaccg accccgagcg gaagcgaaag attatcggcc gtctcttcat
    33901 taagaatttt gaggtcgagt ccaaaaagtt gaagggcatc aaatacctcg tgcaaggtac
    33961 gttgtatccc gatgtcatcg aaagcgtgag cttcaagggc ccctcggcga cgatcaaaac
    34021 gcaccacaac gtcgggggat tgccgaccag aatgaagttg aagctggtcg agcccctgcg
    34081 tgaattgttc aaggacgaag tgcgggtctt gggccaggag ctcggcctgc cggatgagat
    34141 tatctggcgg caaccctttc ccggtcccgg cctggcgatc cgcgtgctcg gcgcagtcac
    34201 caaggaacgg ttggcgattc tgcgcggggc ggaaatgatc gtcgatcagg aaattcgcgc
    34261 cgcgggactg tatcgagaga tctggcaggc cttcgccgtc ctgctgccga tccggacggt
    34321 cggtgtcatg ggtgaccagc ggacgtatga atatgtcatt gccatccgcg cggtcacgag
    34381 ccttgatggc atgaccgccg attgggccaa aattccgagc gatgtgctgg ggaagatgtc
    34441 gagccggatc atcaacgagg tcaagggcgt gaatcgcgtc gtctacgaca tcagttccaa
    34501 gccgccttcc acgatcgagt gggagtaacg caagcgtgaa gcgtcattca taacgcgtca
    34561 ggcgatggat acatatccga tctaatccct cgaatgacga gagacgcttc acagaaagtt
    34621 tgaatggaag tcagactaca aaaactcatc gccggcaccg gactggcttc acgacgcaaa
    34681 gccgaggagt tgatcacgtc cgggcgcgtg acgatcaacg gtagcgtggt aaaggaactc
    34741 ggcaccaaag tcgatcccgc tcgcgaccat gtcaaagtcg atgggaaaca tcttcgggct
    34801 gcgcagccct acgtgtattt gatcctcaac aagcccaaga acgtcatgtc cacgctggat
    34861 gatcctgagg gacgtcccac ggtgaaggac ctcctgcacg gagtcaccgt gcgcgtgttt
    34921 cctgtcggcc gcttggattt cgacagcgaa gggctcatgt tgctcacgaa tcacggcgac
    34981 ctggcccagg cgctgctcca tccccgctac catgttccaa aaacctattt gatcaaggtc
    35041 aagggtgtgc tcgacgatgc gaagatcgaa gcccttgaac gaggcgtcaa gctggaagac
    35101 ggcttcacgg cgccggcgaa ggtgaataag gtcagcaagg cggagagcaa ttcgtggctt
    35161 gaggtcacga ttcacgaagg acggaaacat caagtgaagc ggatggtcga ggcggtgggc
    35221 cattcggtga ttaagctcgt gcgtgtccgc atgggaccgt tgcttttggg agatttggcg
    35281 ccgcgagaat atcgattcct gaccgaccgc gaggcgaatc ggttacggga tctggtggag
    35341 gatcgggtgg ccagagcgga acgtccggaa ctggacgcgt ccggaaaacc gactcgatgg
    35401 atgccgccga cggagccggc tccgcctcgt cctcccaagc ccgagcgttc agggcgcggg
    35461 ccaaaagctc agcgttccga acgggggccg cgatctcaac ggtctctggc cggtgcgaaa
    35521 ccttcacgct caggtcgcgg aaccacgcgg cggcccatga gtcccggtgc caaatttcgt
    35581 cgtcctgcca aaggggttgg ttttcaacgg actcaggctg gtccgatccc gcagggggct
    35641 gatcgtggcc ctggctcgcg tccgaccggg ccagaccaaa aaccccagcg cactcagcct
    35701 ggagcaggat ttcagcggcc ccggtccggt gcgaaacctc aaggccccgg tcggggcatg
    35761 acggttcggc gctcgggtcc tggtcagaac tttcaacgga agcggcgcgg cggatcgtca
    35821 gcttcgcgcc cgggacaacg aggccctgct ggaaaatcgc ggccctcaac gcggagaaga
    35881 gcatgaagat caggaccca
//
LOCUS       DAORQN010000005        34618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_72_length_34618_cov_41.965107, whole genome shotgun sequence.
ACCESSION   DAORQN010000005 DAORQN010000000
VERSION     DAORQN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_34618_cov_41.965107"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1759
                     /gene="aspS"
                     /locus_tag="PK614_00870"
     CDS             2..1759
                     /gene="aspS"
                     /locus_tag="PK614_00870"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRB80799.1"
                     /translation="MRTNRCGELTKAQVGQQVVLNGWVQRRRDHGMVLFIDLRDRTGL
                     TQVVFNAERNSAVHQAAHTLRSECVVSVTGQVMARPDESKNPNLPPGEIEVFVDAVEI
                     LNEAKTPPFMIEDDIEITESLRLKYRYLDLRRPRMQRLLGLRHGIMQAVRAFLNAEQF
                     LEVETPVLTKSTPEGARDYLVPSRVNPGMFYALPQSPQLFKQVLMVSGVDRYYQIARC
                     FRDEDLRNDRQPEFTQIDLEMSFVDREQVMTLMERMIVSVFKDAGGVQLPAPFPRMTY
                     ADAMGRYGSDKPDLRFDMPLHDVTAFAAKSEFKVFKEAATKGGLVKALIVSGGASIAR
                     SRIDALGETAKSFGAKGLAWLKVTADGQLESVIAKFLDAKAFAEALPEAKPGDLVLFG
                     ADKPAIVHDVLGRIRLLLGEELNLIDKTAWKPLWVTEFPLLDYSPEEKRYIFMHNPFA
                     APMDEDLPFLDTEPLKARAKAYDMVLNGSEIGGGSIRNHRSDIQLRILDLLGIGKEQA
                     QAKFGFLLEALEYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQRAQCPLTEAP
                     SGVSAEQLKELRIKLDLVE"
     gene            1778..2914
                     /gene="aroC"
                     /locus_tag="PK614_00875"
     CDS             1778..2914
                     /gene="aroC"
                     /locus_tag="PK614_00875"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRB80800.1"
                     /translation="MAGNTLGRVFTVTSFGESHGPAIGCVVDGCPPGLALSAEDIQRD
                     LDRRKPGTSRHVTQRQESDTVEILSGVFEGKTTGTPIALLIRNEDQRSRDYGNLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRSSARETAVRVAAAAIARKWLQEKHGVVIRGYLSQL
                     GSIEAPFQSWETVDTNPFFSANAEVVGTLESFMDELRKAGDSVGAKITAVAEHVPVGW
                     GAPVYAKLDADLAGAMMSINAVKAVEIGAGFASVAQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDVVVTIAIKPTSSIRIPRRSIDKQGNPVTLETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNADVQTATPKIPGSSPYGAVPVSKKPTA"
     gene            3025..3495
                     /gene="rnhA"
                     /locus_tag="PK614_00880"
     CDS             3025..3495
                     /gene="rnhA"
                     /locus_tag="PK614_00880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRB80801.1"
                     /translation="MIEIYTDGACSGNPGPGGWGVLLRIDNAETELCGGDPATTNNRM
                     ELLAVIEALQSLTQPVEARVYTDSQYVQKGISEWIHSWKRRGWKTAGKEPVKNEDLWR
                     RLDTLASGHKLEWHWVRGHNGHPENERVDALARAGLEQSRRARQTVGGTRPGTL"
     gene            3538..4335
                     /locus_tag="PK614_00885"
     CDS             3538..4335
                     /locus_tag="PK614_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB80802.1"
                     /translation="MAISSPILDIQHATVYRGDTLVFSDFSFVLQAGEHAAIVGPNGA
                     GKSTLLKMLSGEVHSVPLDHTRVRLFGEERWNVWDVRKKLGIVSYDLQRDYLICAEGR
                     NVVLSGFYASNDTYDHQTFTRDQVSRADEVMRELGIESFATRVFGHLSTGEQRRFLLG
                     RALVHDPPVLVLDEPTSGLDLKSCFQYLDLVRAQIHKGKTIVLVTHHLHEIPPEIDRV
                     IFLKGGKVMADGPKLGLLTNDHISSLFDRPIVLAQANGWYQALPGLS"
     gene            complement(4345..5379)
                     /locus_tag="PK614_00890"
     CDS             complement(4345..5379)
                     /locus_tag="PK614_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB80803.1"
                     /translation="MSRSSLDYVKQIVRGSIGMMAAVSLATGIFTNEAAAVEATMLER
                     AKLATIGVLEDTQDQRTPDKPGKILVRGTGFHLRDGYIVTARHAAEKQDVTTGTVIQK
                     QIHVLTTDLHEIPADLVGDSAFMDVVVYRVAEAHRAKLRATVPFASGDVQPGQEVFTV
                     GYPMGWGPTMSFGHLGNTNTFLQTVDTRLIQADVAACSGNSGGGLFNDKGEVVGIMHA
                     IIQTERDDSTAHCSRMAFAIPAMLAERIVTAALDGKPMAFSKMGIHMIAVKDGTRWRM
                     AVKDVMEPAKSAGIEKHDIIIAVDDTDIQDAAHLKTYLIERTKPGQQVSVKVRRIDTN
                     LTFTVTLAGG"
     gene            5500..6090
                     /locus_tag="PK614_00895"
     CDS             5500..6090
                     /locus_tag="PK614_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HRB80804.1"
                     /translation="MASSRPHGYTELMLLRARLYLMIGLLAVTVGCATSVIPESLEPQ
                     IDKAVTFSEVLESPDSYRGKVVVWAGEVLKAKAMKDGTQLEVLQLPLDDEQGPVTDRM
                     ESKGRFLALQHEFLDPATMTDGTRVTIVGEITGASVEKIDEADYRFPMLDVKHLHRWD
                     SRQVDDRRVIGPWWNVFGGVGFGGGSRSGGGISIGF"
     gene            complement(6108..6323)
                     /locus_tag="PK614_00900"
     CDS             complement(6108..6323)
                     /locus_tag="PK614_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80805.1"
                     /translation="MTLGLVIGTQAMPRIAPLKNAEGKTTDRGALEQQVTQNLTTIRN
                     GAEGVKEFLTGYLLSAGAGTGDILLGI"
     gene            complement(6334..7200)
                     /locus_tag="PK614_00905"
     CDS             complement(6334..7200)
                     /locus_tag="PK614_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRB80806.1"
                     /translation="MRWEGQRESGNIEDRRGMSVGRAGAGLGLGGIVLVLAVSFLTGT
                     NPLTLLNLLNGVQEITAPAEPEHSGPKGAPRDSLGKFASVVLADTEDTWRTLLPRLGR
                     TYEDPQMVLFTGAVRSACGTASSAVGPFYCPGDHKVYLDLSFFNEMAQRLGAPGDFAQ
                     AYVIAHEIGHHVQNLLGIAEKVTGLQQRGSTAERNALSVRMELQADCFAGVWGYHAQH
                     NRNLIEPGDFEEGLRAAAAIGDDRLQKLGQGQVQPESWTHGSSEQRMTWLRRGLQSGD
                     PNTCDTFSGGRA"
     gene            7540..8118
                     /locus_tag="PK614_00910"
     CDS             7540..8118
                     /locus_tag="PK614_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80807.1"
                     /translation="MRARFIVLALTGLLGLFGCQSPGTPLPPTLKPSLAWPECPKIGG
                     IYELQGEPLPGTMNFYRNRDNKLTLNAMLGVAVPADLAQEAVRVELVHEDTLELVNRF
                     DGAITAHQLDLRPEDRVTCDRNQIRIHRTRSEGGVAEEPLRNISDIAQELTLEGDGSL
                     LVKTRIMNQSKSAFFSTPVPHEEYSARFRRLD"
     gene            8245..8832
                     /locus_tag="PK614_00915"
     CDS             8245..8832
                     /locus_tag="PK614_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB80808.1"
                     /translation="MTVHRAARTLVFNKPYDVLPCFTDPEGRQTLGDYIDVSGVYAAG
                     RLDRDSEGLLLLTSDGALAHRITDPEHHLPKVYLVQVERIPDAAAVERLQQGVVVGGR
                     RTKPARVRLLLEAPQIPERPVPIRFRKHVPTAWLELTLREGMNRQVRRMTAAVGHPTL
                     RLVRVAIGPIFLGALLPGEWRELTKAETAEIYGYS"
     gene            8978..9439
                     /locus_tag="PK614_00920"
     CDS             8978..9439
                     /locus_tag="PK614_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRB80809.1"
                     /translation="MNTRDRIIVGLCGVLLGLLVLVSPAHADDTLVTKQSHYTVAETI
                     DRIARAVTDKGMLLFARINHADEARKVGLEMPPTELLLFGNPRGGTALMLAAPTVAID
                     LPMKALAWQDQVGRVWLTYNAPALLPVRHGLAAELAARLDPVGTLLERAVE"
     gene            9622..10164
                     /locus_tag="PK614_00925"
     CDS             9622..10164
                     /locus_tag="PK614_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80810.1"
                     /translation="MRSQTIAVGRVSLLLVLCGIGLLPTVSALGANPESVPLSDMRHI
                     KKFVAVEVQTQGTAEKLGINGAELTDVTRATLLNKVPGIALESSSGPSSDAPERLNQL
                     GFFTCEVWTVGEQYIAAYHVDCNAGSYTAQKTPGSLWNQAILGYGPRDEVSDAVRKGV
                     RAMVELFANTFANARMDAGR"
     gene            10378..10857
                     /locus_tag="PK614_00930"
     CDS             10378..10857
                     /locus_tag="PK614_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80811.1"
                     /translation="MADASSGQRISHPYGWLLLGLLLLFFFRVAAQLIQAYSPVTSLP
                     PFETWQSGALPYPLLVSLQLIILVACGRVVWSFLSGTVVPSGKKGRLLLVLGWVYFVV
                     MGIRLLIGLTVAPNHFWFGAIVPTLFHLVLASFLLVSGSFHTASGSCSVAPVSEGAA"
     gene            10854..11729
                     /locus_tag="PK614_00935"
     CDS             10854..11729
                     /locus_tag="PK614_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRB80812.1"
                     /translation="MILLLRYGAYPSILLACVIGNLVLFHMGTGVLPATYLPVTLGTL
                     LIMALEATLPYRSDWRPSRVEVVQDSTFLALVHVILPKALAAASVVVIFDVLHGRGWG
                     MSVWWPHDWPVPTQTMLMVLIVDGLRYWLHRLSHEWEPLWRVHAVHHAPQRLYTLNVG
                     RFHPIDKGLQFVFDALPFLALGVREDVLSLYFVWYAVNGFFQHSNVDVRLGWLNYVVS
                     GPELHRWHHSMQKEESNHNYGNHLIVWDVVFRSRYLPADRSVGALGLVNRQYPRGFLD
                     QMAAPFTPGLDKQTV"
     gene            11738..13375
                     /locus_tag="PK614_00940"
     CDS             11738..13375
                     /locus_tag="PK614_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRB80813.1"
                     /translation="MSRFLHDPALSLSLTLTKYRDWNPFIAHTHCPELAQQAALRDIL
                     ARQAGTAFGRKHRFGSLLRYEEFAREVPVCTYEDLRPAIEVQEKNREPLLTSGQPILF
                     TQTSGTTGVPKNIPILPQTVEAIRRYQGLFAYAQWQGVPAIYQGRVLVISGQTIEGHL
                     PGGTPFGSMSGLMFDCLPAAIRRKSILRGGESAASDYRQRYFNIAVRALADPSLSVLA
                     TPNPSTILKLLEVIRGEYALLLEALSGETRGGCLPPFEKLPISSTRLSQLRVFIGQEE
                     ALDCATLWPNLRAVVTWTGGNCAVLIPKLGLLLSPQTRIIEMGYLSSECLGTVNVDVL
                     NNRCVPTLADNLFEFVEVGDEASDIEPVLLHQLQAGRKYTVIVTTRNGLYRYAMNDII
                     EVTGYFNRTPTIRFVQKGKGVTNITGEKLYEHQVTEAVGQALIARGLSSEFFVMLADV
                     ENSRYTLCLEHAWSLGDLGILVEERLSSMNIEFKAKRASGRLQPIHVLQLRPGTGDVY
                     RQHCVSQGQREAQFKLIRLQYAHDSTFDFERHGEVMT"
     gene            13375..14469
                     /locus_tag="PK614_00945"
     CDS             13375..14469
                     /locus_tag="PK614_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRB80814.1"
                     /translation="MRMRSLTMTSLEIPFRQAFTHASATRTTTEAVLVRAESARGLVG
                     MGEGCPRHYVTGETVASAQEFFRSHRAEWMICSSMNDLQTWGTAHADLIDRNPAAWCA
                     VETACLDLLGKELEQPIEVVLGGTPLSGPFRYSAVLGGEKFSSFEKQLRQYLAAGFID
                     FKLKVMGDLEADRERVAALTAAGTPGLRVRLDANNRWHRVDEACEYIQSLQGSFTALE
                     EPLRVGDYEGCRALSRHCGVPIILDESFVKVGQFPLIEGEPSLWILNIRVSKMGGLLR
                     ACTVAARAKAAGIPIVVGAQVGETSILTRVALVVADRYRDILIAQEGAVGTHLLEYDL
                     CEPPLMFGRGGSLTDIFFRDRPGLGLSFAP"
     gene            complement(14536..15546)
                     /locus_tag="PK614_00950"
     CDS             complement(14536..15546)
                     /locus_tag="PK614_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB80815.1"
                     /translation="MKRRNLLKLTGIAAVAGLTGGCDAVGSLFGRMFAMPPRETNYFT
                     ENKKFYVVNYSDSPFNVSRELRQDEWRLSVKGEVKKPLSLGWRELLNRNNFEQISTLM
                     CIDTLPGGDSLGNARWRGISLKKLLQEAEIDEETTRDIVFRGADAYDDSIPLTRAMQD
                     DVMLAFLMNGEKLPKEHGFPLRLLVPGLYGIKNVKWIVEIEAYAGDYRGYWQRKGWTD
                     DATIKTFSRIDSPGHYQTLRGPEQRFRGIAFGGPHSISRVEISLDATRTWDSCEIETP
                     MSPYSWVIWNYTWRPSKRGKYQVAVRAIDSKGHPQIAELIRPQPAGSSGLHTIVADVE
                     SL"
     gene            15886..17419
                     /locus_tag="PK614_00955"
     rRNA            15886..17419
                     /locus_tag="PK614_00955"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            17592..17668
                     /locus_tag="PK614_00960"
     tRNA            17592..17668
                     /locus_tag="PK614_00960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17626..17628,aa:Ile,seq:gat)
     gene            17733..17808
                     /locus_tag="PK614_00965"
     tRNA            17733..17808
                     /locus_tag="PK614_00965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17766..17768,aa:Ala,seq:tgc)
     gene            17929..20935
                     /locus_tag="PK614_00970"
     rRNA            17929..20935
                     /locus_tag="PK614_00970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            21062..21178
                     /gene="rrf"
                     /locus_tag="PK614_00975"
     rRNA            21062..21178
                     /gene="rrf"
                     /locus_tag="PK614_00975"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            21378..22058
                     /locus_tag="PK614_00980"
     CDS             21378..22058
                     /locus_tag="PK614_00980"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRB80816.1"
                     /translation="MLPPIPSSWYQFFTEQINGNNFRALETFLDREAEAGETILPPSQ
                     DIFGALEATPYEQVNVLLLGQDPYHTPGMAHGLCFSVRPDVPVLPRSLRNIYRELRED
                     VGCSVPNNGCLEPWARQGVLMLNAVLTVRAHAANSHRGRGWEAVTDRIFAVLNAKPSR
                     VVFLLWGAEAKKKQSLITNAQHVVISCAHPSPLSARKFFGSRCFSQVNRALTEVHLKP
                     IDWQIPDL"
     gene            22195..22983
                     /locus_tag="PK614_00985"
     CDS             22195..22983
                     /locus_tag="PK614_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRB80817.1"
                     /translation="MRIRLGLGLGFSTLVVAGTLLAAEGDLAGLRTPTSIGSAPVAWQ
                     YDGDQGPSHWGEIAPTTASCEKGTHQSPINIRTTPHHQSHDGLLVHYTAAPGHVVTSH
                     HTIEVDFQSGESLEVLGRTYTLKEFHFHEPSEHQLNGRTYPMEAHLVHRDETGHLVVL
                     AVLMDLGNESASLSAVWDRIPSEKQDEVRDLLINPQDLLPKDLHHYAYDGSLTTPPCT
                     EGVHWIVLKEPTSITSAHIERFVSLIGHNARPVQSLNEREIDEE"
     gene            complement(23350..23736)
                     /locus_tag="PK614_00990"
     CDS             complement(23350..23736)
                     /locus_tag="PK614_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80818.1"
                     /translation="MSNGQDSQTSPVARWVRIPNGTKVRHRHKGYDGHIDGLTEIVVG
                     PNRNPDGKTQYRLNIGGSSRELVTEDDLSILVDGDNLVIMIRQKEPYRRSITAQLHGV
                     LAEDRFIKAGRSSAVPATTPPQVPSA"
     gene            24568..24705
                     /locus_tag="PK614_00995"
     CDS             24568..24705
                     /locus_tag="PK614_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80819.1"
                     /translation="MMAQMVLFWAAHNLFAVVTICFGGAMLSVMLYTYLHDYVLVAKG
                     R"
     gene            24753..25040
                     /locus_tag="PK614_01000"
     CDS             24753..25040
                     /locus_tag="PK614_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80820.1"
                     /translation="MRIHPTITTRVLSLFLATAWLLAVSLTVQASEQKPVATVAPVKS
                     VAVASGAVEDTLPMCLTRIPQEATPGQRLIAEQSCQRDESARASMQNVPGR"
     gene            25265..25477
                     /locus_tag="PK614_01005"
     CDS             25265..25477
                     /locus_tag="PK614_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB80821.1"
                     /translation="MPVGVGRNMLLDINRAEISRLKSLTGIGEHYARKIVEGRPYHHR
                     EELLTKEILPEYIYGRIMDRLVANQS"
     gene            25675..26283
                     /locus_tag="PK614_01010"
     CDS             25675..26283
                     /locus_tag="PK614_01010"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB80822.1"
                     /translation="MALVAAISLLMGIVQATAAWSEQGPTEAVKGTVSELLSILKELK
                     DPTRSEARRWEIEQVIRHHVHYEDMAKRSLGASWGQLSDAARREYVGLFVQLLRDALA
                     NRMVEYSGERISYLSEQRERTFAQVKTRLVGNKVDTFIDFRLVSQDGRWLVYDAVMDG
                     GSLVASYHAQFASIIRDASCAQLMERIKEKTLVVKLFEKSGS"
     gene            26296..26526
                     /locus_tag="PK614_01015"
     CDS             26296..26526
                     /locus_tag="PK614_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80823.1"
                     /translation="MARSRAVTSPGESAMRCPRCYGPMMMIKMKDTVSGTLAYGWGCR
                     QCGAVIDSLIDIHRQWHLEPRATWAHLAVAAC"
     gene            26631..26798
                     /locus_tag="PK614_01020"
     CDS             26631..26798
                     /locus_tag="PK614_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80824.1"
                     /translation="MLHAALIALALGLVVWGLNWTGLAEMPTQVYWVFTVGGMLLMLI
                     HVATRRPARVW"
     gene            26846..27403
                     /locus_tag="PK614_01025"
     CDS             26846..27403
                     /locus_tag="PK614_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030089 - phycobilisome [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phycobilisome rod-core linker polypeptide"
                     /protein_id="HRB80825.1"
                     /translation="MLGIGAFCFSEAEAAKEWAPQEVAAILAQSYAQLLMRPMGTEPS
                     SLQKNEADQQRYMVQSGYASQLARGEKSVKQIVEAIALSSEFNAKWITPHMGTAQTGS
                     APGVSPVAAPEKAIDSLYCALLGRRVDSASLASARKDLVSSGFERVIRDVIDGKEYRD
                     RFGDTKVPQVSSEKQAAVGCPQVAL"
     gene            27494..27682
                     /locus_tag="PK614_01030"
     CDS             27494..27682
                     /locus_tag="PK614_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80826.1"
                     /translation="MSMTPEERIANMTKAINQAIKVMGDRFGSTEEDVARAIQGLKAS
                     MQTAPQEKPVPVGAATQA"
     gene            complement(27867..28064)
                     /locus_tag="PK614_01035"
     CDS             complement(27867..28064)
                     /locus_tag="PK614_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80827.1"
                     /translation="MELLVTTPLLAGSDNRSVHEDGIIGQCRALWRKKRRRPDDQSRG
                     MEARSARERTVGGAKDVMPSR"
     gene            28111..28431
                     /gene="erpA"
                     /locus_tag="PK614_01040"
     CDS             28111..28431
                     /gene="erpA"
                     /locus_tag="PK614_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRB80828.1"
                     /translation="MITITATAEEKIRELMLEEKDTLGLRVYVKGGGCHGYQYGMAFE
                     SKMSDDDTVIEKGDVKVIMDSQSAPLLSGCEVDYVDSVQGSGFAIKNPQAKTTCGCGS
                     SFSA"
     gene            28489..29694
                     /locus_tag="PK614_01045"
     CDS             28489..29694
                     /locus_tag="PK614_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB80829.1"
                     /translation="MEPTSVIGTKNKKGQVFTDPRIMMREAPRTPSFYTGSEVIKEAI
                     RRASIDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGARV
                     FTTTAGPGTMRAMENFPMWAGARLPIQCIVTCRGINSPLSIQPDTIEISYLMQTGMLV
                     WHAETAQDFYDWILMGYMVSEEPEVHLPLALCCDGFFVTHTKDVVDLTPTDMCLPPYD
                     PYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEVWAAAERSRKHTIAWLN
                     GLIETEDVDADVLIVTSGTAVSQGREAIRLLADEGIRVGLVKIKTLRPWPEEEIKEAT
                     KHATHIIVPEFNVIGWMAKEIKATIPRSERVIAGPRVCGGMTMPPEIIVEEIKRALGV
                     RSGTLAGRG"
     gene            29777..30673
                     /locus_tag="PK614_01050"
     CDS             29777..30673
                     /locus_tag="PK614_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB80830.1"
                     /translation="MSKERIKISPDLYDIMPSDYQDLVSSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPDRIKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     VRNAVLIAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAFKEYISEPAK
                     QLLAERDAKAKEKKAAAKQLVS"
     gene            30726..31619
                     /locus_tag="PK614_01055"
     CDS             30726..31619
                     /locus_tag="PK614_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB80831.1"
                     /translation="MSLDYVKFTPGFEQFMPKEYRDMVEHGPFGKKTTVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAIIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVIAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVTMAKVAGCAYIATVVPNNPRRVESVI
                     KKAVLIARDIGPTYIQAYTSCNIEYAIPTDKVMEDAKTVEDDRYKFTEYVSDEAKAYL
                     AERYGYKEFMPKAVPASVTTA"
     gene            32063..32305
                     /locus_tag="PK614_01060"
     CDS             32063..32305
                     /locus_tag="PK614_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80832.1"
                     /translation="MTQEQFQQFWLQLKAPLKANWDKITESDLGEIQGSLVKFGDVLQ
                     KRYGEGHKDEVSLWADRRHAHWSGNYIGYKDPKPAV"
     gene            32396..32740
                     /locus_tag="PK614_01065"
     CDS             32396..32740
                     /locus_tag="PK614_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80833.1"
                     /translation="MRKIVINKSYDQFCLSHKAFLRLRELGQPDALKETDLGAYWPSA
                     AGPQEPSLNQCGMLIPRDDKRLVQVVEELQGEANGHCAQLKVVSIPDDVSWVIAKANG
                     SEHVSEQHRTWS"
     gene            complement(33104..>34618)
                     /locus_tag="PK614_01070"
     CDS             complement(33104..>34618)
                     /locus_tag="PK614_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRB80834.1"
                     /translation="VAEGILAYEAKRYDEAAVLFSRAVATDPRNARAFYYLALCHLAR
                     GQAELAIAPLTALHTLRPSDPEATYQLGVAHFATKNYDKAAPLLEDVFRLEPDRENLG
                     FHVGFLRYRQKNYNGAEAALSVSRSADQDIRQLTMFYRGLALGILGLSDQAQAELASA
                     QRVQPTSPITGASVRIQEALTATTRVTDTQRLRLQLAVGGYYDDNVAVNPRKSSEQVA
                     ELLRSRNTQSPGFVTSARGDYAWYRKGPIESTVTYSYYQTVNTDSGAGSFNIQDHLGS
                     LSGVYRGTVGAMPYEIGGQYSYDYMLLGLKGFLSRHTVTLPATFVPPAITLPALGRMD
                     NLTTLLYRFQRKSFFTEPGNSDIRFAPESRDAFNNMVGVLHAFRFRQDKFIVRVGYQH
                     DTEAASGSSFAYSGNRAQLGGQTTLPWYDLSLRVDYEVHWRAYNHAQVIFLDDAGQLS
                     RRRDTEQDIFVQIARPLFRSVTVALQYQGVLNGSNIPVYAYDKNVFTTLLTWTY"
BASE COUNT         7288 a         9033 c        10728 g         7569 t
ORIGIN      
        1 gatcaggacc aatcggtgtg gggaattgac caaggcgcaa gtcggtcagc aagtggtgtt
       61 gaacggatgg gtgcaacgtc gccgggatca cggcatggtg ctgttcatcg atctgcgtga
      121 tcgaacaggg ttgacgcaag tggtgttcaa cgccgagcgg aattcggcgg tccaccaagc
      181 cgcacatacc ttgcggagcg aatgcgttgt gtcggtgacc ggccaggtga tggccaggcc
      241 ggatgagtcc aagaatccga accttccccc cggtgagatc gaagtgttcg tggatgccgt
      301 cgagattctc aacgaagcca agacgccccc cttcatgatc gaggacgaca ttgagatcac
      361 cgagtctctt cgattgaaat accgctatct ggatctcagg cgcccgagga tgcagcggtt
      421 gctgggattg cgtcacggga tcatgcaggc ggtgcgggca ttcttgaatg cggagcagtt
      481 tctggaagtg gaaacgcccg tgctgaccaa aagtacgccg gaaggcgctc gtgattatct
      541 ggtgcccagc cgcgtgaatc cggggatgtt ttatgcgctg ccccagtcgc cgcagctgtt
      601 caagcaggtg ttgatggtga gcggtgtgga tcggtattac cagatcgccc gttgcttccg
      661 cgacgaagac cttcgtaacg atcgccagcc tgaatttacc cagattgacc tcgaaatgtc
      721 gttcgtggat cgtgagcagg tcatgacgct catggagcgc atgatcgtct ccgtattcaa
      781 ggacgccggc ggggtacagt tgcccgcgcc ctttccccgg atgacctatg ccgacgccat
      841 ggggcgctac gggtcggaca agccggacct tcgcttcgac atgccgttgc atgatgtcac
      901 ggcgtttgcc gcgaagagcg aattcaaggt gttcaaggag gcggcgacca agggcggact
      961 cgtcaaggcg ctgatcgtgt ccggaggggc ctccattgcg cggagtcgta tcgacgcgct
     1021 cggtgaaacg gcgaaaagtt ttggcgcgaa aggcctggcc tggttgaaag tcactgcgga
     1081 cggccagtta gaatcggtga tcgccaaatt tctcgatgcc aaggcctttg ccgaggcctt
     1141 gccggaagcg aagcccggcg atctggtgct cttcggcgcg gataaaccgg ccatcgtgca
     1201 cgatgtcttg gggcgaatcc gactgctgct tggtgaggaa ctgaatctca tcgacaaaac
     1261 agcctggaag cccttgtggg tgacggagtt cccgctgttg gactattcgc cggaagagaa
     1321 acgctatatc ttcatgcaca atcccttcgc cgcgccgatg gatgaagacc tgccgttcct
     1381 cgacacggag ccgttgaagg ccagggccaa ggcctacgac atggtgctca acgggagcga
     1441 aatcggcggt ggtagcattc gcaatcatcg tagcgacatc cagttgcgga ttctggatct
     1501 gttggggatc ggcaaggaac aggctcaagc gaagttcggc ttcctgctgg aagcgctgga
     1561 gtatggcgcg cctccgcacg gcggcattgc gttcggcctc gaccggttga tcatgctgct
     1621 gggtggcgcc gattcgattc gcgatgtcat cgcgtttccc aagacgcaac gcgcgcaatg
     1681 cccgctgacc gaagcgcctt ccggcgtgag tgccgagcaa ttgaaggagt tgcgcatcaa
     1741 gttggatctc gtcgagtagg taggagtcat ccaacccatg gccggcaata cgttgggccg
     1801 agtcttcact gtcacctcgt tcggcgaaag ccatggaccg gcgatcggct gtgtggtgga
     1861 cgggtgtccg ccgggccttg cgctatccgc cgaagacatt cagcgagatc tcgaccggcg
     1921 caagccgggc acctcacgcc acgtgacgca gcggcaggaa tccgataccg ttgaaatcct
     1981 ttccggggtg ttcgagggca agacgaccgg tacgcccatc gccctcttga tccggaacga
     2041 agaccagcgc agccgtgatt acggcaacct catcgacacc tttcgtccgg gccacgccga
     2101 ctacacctat tggcagaagt acgggatccg cgaccatcgc ggcggggggc gctcctcggc
     2161 gcgtgaaacg gccgtgcgag tggctgcggc agcgattgcc aggaagtggt tgcaggaaaa
     2221 gcacggggtt gtcatccgtg gctacctcag ccaactcggg tcgatcgaag ctccctttca
     2281 gagctgggaa acggtggaca ccaatccatt cttttcggcg aatgctgagg tggtggggac
     2341 gctcgaatcg ttcatggacg agttgcggaa ggccggcgat tccgtgggcg caaagattac
     2401 cgccgtggcc gagcatgtgc cggtcgggtg gggtgccccg gtgtatgcga aactggatgc
     2461 tgacctggcc ggcgccatga tgagcatcaa tgcggtcaag gcagtggaga tcggcgcagg
     2521 gttcgcctcg gtcgcgcagc ggggttcgga gcatggcgat gaactcacgc ctgaagggtt
     2581 tgtgacgaat catgccggcg gcattctcgg cggtatttcc accgggcagg atgtggtcgt
     2641 gaccatcgcc atcaagccga cctcaagcat ccgtattccg cgacgttcca tcgacaagca
     2701 gggcaacccg gtgaccctcg aaaccaatgg gcgtcacgat ccttgcgtcg gtatccgggc
     2761 cacgccgatt gctgaagcca tgatggccct ggtgctgatg gatcacgcac tcttgcatcg
     2821 cgcgcaaaac gccgatgtgc agacggcgac gccgaagatt ccaggttctt caccgtatgg
     2881 tgctgttccg gtgagtaaga aaccaaccgc atagatcaag cctgatcctg cgaagccgag
     2941 tcatcttcgg tctgactcgt gtgtgtgatc ggcgagctac gtgtcgttcg ttatacctta
     3001 tgaacgaaag aggactgtga gccaatgatc gaaatttata ccgacggggc ctgcagcggg
     3061 aacccaggac ctggcgggtg gggtgtgttg ctgcgtatcg acaatgccga gacggaattg
     3121 tgcggcggag atccggccac gacgaacaac cgcatggaac tgctcgccgt cattgaggcg
     3181 ttgcagtcgc tcacgcagcc ggtggaggcg cgtgtctaca ctgattcgca gtatgtgcaa
     3241 aaggggatca gcgagtggat tcatagctgg aagcggcgcg ggtggaaaac cgccggcaaa
     3301 gagccggtga agaacgagga tttgtggcgc cgtcttgata cgctcgcctc cggtcacaag
     3361 ctggaatggc actgggtgcg gggtcacaac ggtcatccgg agaacgaacg ggtggacgca
     3421 ctggcacgtg ccgggctcga acagtctcgc cgggccaggc agacggtcgg cggtacgcgg
     3481 ccgggcacgt tgtagtgcat atgcgatacg ctacgcagtg accatctaac ccgctgtatg
     3541 gcgatctcct ccccgattct cgacattcaa catgccacgg tctaccgagg cgacaccctc
     3601 gtcttctcag atttctcctt cgtcctccag gcaggagaac atgccgccat tgtcggtccc
     3661 aacggggcag gcaaatcgac cctgctcaaa atgttatcgg gtgaagtcca ctcggttccg
     3721 ttagatcata cgcgggttcg tctgttcggg gaggaacgct ggaatgtctg ggatgtgcgc
     3781 aagaaactcg ggatcgtttc ctacgacctt cagcgggact acctgatctg tgccgagggg
     3841 cgcaacgtcg tcctctcagg cttctacgcc agcaacgaca cctacgacca ccagacgttt
     3901 acgcgagacc aggtaagccg cgccgatgag gtgatgcggg agctaggcat cgaatcgttc
     3961 gccacgcgtg tcttcgggca tctctcgaca ggggagcagc gacggtttct tctcggccga
     4021 gcgctggtcc acgatccgcc ggtcctggtg ttggacgaac cgaccagcgg gctcgatctc
     4081 aaatcctgct ttcaatactt ggacctggtg cgcgcacaga ttcacaaagg caagaccatt
     4141 gtgctcgtca cccatcatct gcatgaaatc ccacccgaga tcgatcgcgt gatctttttg
     4201 aaggggggaa aggtgatggc cgacggcccg aaattaggcc tgttgaccaa cgaccacatc
     4261 agcagcctct tcgaccgccc catcgtgctc gctcaagcca acggctggta tcaagccctg
     4321 cctggcctgt cgtgacaggc cggatcatcc ccctgccagc gttaccgtaa aggttagatt
     4381 ggtatcgatg cggcggactt tgacgctgac ttgttgcccg ggcttggttc gttcgatcag
     4441 ataggtcttt aaatgcgccg catcctgaat atcggtgtca tcgacggcaa tgatgatgtc
     4501 gtgtttctca atgccggcgg acttggccgg ctccatcacg tctttcaccg ccatccgcca
     4561 cctggtgccg tctttcacgg cgatcatgtg aatgcccatc ttcgagaagg ccatgggctt
     4621 tccgtccagg gcggcggtga cgatgcgttc ggccagcatt gcgggaatgg caaaggccat
     4681 ccggctgcaa tgtgcggtcg agtcgtcccg ctcggtctgg atgatggcgt gcatgatgcc
     4741 gaccacttca cccttgtcgt tgaagaggcc gccgccggag ttgccgctgc acgcggccac
     4801 gtcggcttga atcaaccggg tgtccacggt ctgaaggaag gtgttcgtat tgccaaggtg
     4861 tccgaacgac atggtcgggc cccagcccat ggggtagccc acggtgaaga cttcctgccc
     4921 cggttgcacg tcgccggagg caaacgggac cgttgcgcgc aacttggcac gatgggcctc
     4981 ggcgacgcga tagaccacga cgtccatgaa ggcactgtcg ccgacgaggt cggccggaat
     5041 ttcatggagg tcggtggtca ggacgtgaat ctgtttctga atgacggtgc cggtcgtgac
     5101 gtcctgcttc tccgctgcgt gccgggcagt gacaatatag ccgtcgcgca gatgaaagcc
     5161 ggttccacgc accagaatct tgcccggttt atccggtgtg cgttggtctt gcgtgtcctc
     5221 caggacaccg atggtggcca gtttggcgcg ctcgagcatc gtggcctcga cggctgccgc
     5281 ctcgttcgtg aagatgcctg tggccagact cacggcggcc atcatgccga tcgaaccacg
     5341 cacaatctgt ttgacgtaat cgagtgacga acgcgacata gggaaccgtt cctttcgtga
     5401 ggatgtcgtc agagctgcag tatagcctag aggcgggagc ggatgaaatg tggtcttgcg
     5461 tcagtgtcga tgaacgggcg gctgcgaaga gattctcccg tggcatcctc gcgtccgcat
     5521 ggctatactg aactcatgct gctacgtgcc agactgtatc tcatgatcgg gctgctcgct
     5581 gtgactgtcg gatgcgcgac gtcggtcatt cccgaatcgc ttgagccgca gatcgataag
     5641 gccgtgacat tcagtgaggt cttggaatca cccgattcgt atcgcgggaa ggtagtagtg
     5701 tgggccggtg aagtgctcaa ggcaaaagcc atgaaggacg gtactcaact ggaggtgctg
     5761 cagctcccgc tcgatgacga gcaggggccg gtaaccgacc gcatggagtc gaaagggcgg
     5821 ttcctggcgc tccagcatga gtttctggat ccggccacga tgacggacgg taccagggtg
     5881 acgattgtcg gagaaattac gggggcgtcg gtcgaaaaaa tagatgaggc ggactatcgt
     5941 tttccgatgc tggatgtgaa gcatctgcat cgctgggatt ccaggcaggt ggatgaccgc
     6001 cgggtgatcg gaccttggtg gaacgtgttc ggcggcgtcg gtttcggcgg cggaagccga
     6061 tcgggcgggg ggatcagtat cgggttttga tgaccagccg gtttttttca gattcccaat
     6121 aatatgtcgc cagtgcccgc gccggctgag aggagatagc cggtcaggaa ctctttgact
     6181 ccttccgccc cgttccggat ggtggtcagg ttctgcgtga cctgttgttc gagtgctccc
     6241 cggtcggttg ttttcccctc ggcgttcttc aatggggcga tgcggggcat ggcctgggtt
     6301 ccaatgacga ggcccagcgt cacgaagggg gatctaggcg cgcccgcctg agaatgtgtc
     6361 gcaggtgtta ggatcgccgg actggagccc gcgccgtaac caggtcatgc gttgttccga
     6421 tgatccgtga gtccaacttt ccggctgtac ctggccttgc cccagcttct ggagtcgatc
     6481 atcgccgatc gccgcggcgg cccgcaaccc ttcctcgaaa tcgccgggtt cgatcaggtt
     6541 gcggttgtgt tgggcatggt agccccacac accggcaaaa cagtcggcct gcaattccat
     6601 gcgcacggag agcgcattac gctctgcggt cgagccacgt tgctggagcc cggtgacttt
     6661 ctcggcgata ccgagcaggt tttggacatg gtgcccgatc tcgtgcgcga tgacataggc
     6721 ctgggcaaag tcgccgggcg cgccgagccg ttgggccatt tcgttgaaga aggaaaggtc
     6781 caagtagacc ttgtgatcgc cgggacagta gaacggaccc acggcggacg aggcggtgcc
     6841 gcaggcggag cgcactgcgc cggtgaacag caccatctga ggatcctcat acgtgcggcc
     6901 caatcggggg aggagggttc gccaggtgtc ttcggtgtcg gccaacacca cggaggcaaa
     6961 tttgccgagg ctgtcgcggg gggcgccctt cggtcccgaa tgttccggtt ccgccggcgc
     7021 ggtgatttcc tggacgccgt tgagcaagtt gagcagcgtc aggggatttg ttcccgtcag
     7081 gaagctcacg gcgaggacga gcacgatgcc gcctaatcca aggccggcgc cggccctgcc
     7141 cacactcatc cctcgacgat cttcaatgtt tccgctctcg cgttgacctt cccatcgcat
     7201 ggcaggactc cttgttctat acccgggttc gcgacttcct cgacgaggag tatgaagggc
     7261 tgctcgatgg aaacacattt ggcccggttg agcaagcctg gagagtccct agcacgcagg
     7321 actcacctga gtttccgcca cctccggcct gatggggcgt gtgagtgatg ggcctgcgcc
     7381 agtggcctct gtaaggcagg ataggtggac tgtgtttatg tgacgcacca cgttgatggt
     7441 cctggactgc ctgcataatc cgactagaca ggccggtcat ggcttcgcta gactaatcac
     7501 atgctcttca tgaccagtca gattcatcga caggacccaa tgcgggcgcg tttcattgtt
     7561 ttggcgctga cggggcttct tggcctcttt ggctgccagt caccgggaac gccgctccct
     7621 ccgaccttga agccgagcct ggcttggccg gaatgtccca agatcggcgg aatctatgag
     7681 cttcaaggag aacctctccc cggcacgatg aatttttacc gcaaccggga caacaagctc
     7741 accttgaatg ccatgctggg cgtggcggtg cctgcggatc tcgcgcagga ggccgtgcgc
     7801 gtcgaattgg tgcatgaaga cacccttgag ctggtcaacc ggtttgatgg cgccatcacc
     7861 gcgcatcaat tggatctgcg gccggaggat cgggtgacct gtgatcgcaa tcagattcga
     7921 attcaccgga cccgctcgga aggcggggta gccgaagagc ccctccgcaa catttccgac
     7981 atcgcacagg aattgacgct ggaaggggat ggaagtctgc tcgtcaagac ccgcatcatg
     8041 aatcagagta aatccgcctt cttcagcaca ccggttcccc atgaggaata ctccgcgcgc
     8101 ttccggcgac tcgattagtc gcgatgtcgc gatacgtcct tcgttactgg tcattcctca
     8161 caggtatccc ctcatgatgt tgcgttgctg cttagccact cctgtgatga ggaggactgc
     8221 cgcgacacgg gggggccctt tctgatgacg gttcaccggg ctgcccgcac gctagtgttc
     8281 aacaagccct acgatgtgct gccttgcttt accgatccgg aggggcgtca gaccctgggc
     8341 gactacatcg atgtgtctgg cgtctatgcg gccggtcgat tggatcggga tagcgaaggc
     8401 ctgctgctgc tgacttcgga cggggcgtta gcccatcgca ttaccgatcc cgagcaccat
     8461 cttcccaaag tctatcttgt acaggttgag cgtatccccg atgcggctgc cgtggagcgg
     8521 ctgcaacaag gggtggtggt cggcggaaga cgcacgaagc ctgctcgggt tcgtctgttg
     8581 ctggaagcgc ctcagatccc ggagcggcca gtgcccattc gattccgaaa gcatgtgccc
     8641 accgcatggt tggagctcac cctgcgcgaa ggcatgaatc gccaggttcg acggatgacg
     8701 gcagcggtag gccatccgac attgcgactg gtgcgggttg cgatcggccc gattttcttg
     8761 ggggcgctcc tgcccggtga atggcgagaa cttacgaaag cagagacggc tgagatttat
     8821 ggttattcct gaagagaggg cgtacctgat ggcagggcgt tcgcccggat tattcatgaa
     8881 tgccgtcgcg aggcgtccga gacaagcctc gccgggcgag aggatcggac gccggggcag
     8941 gtattcatga atagtccggg ttaaggaggc agctcacatg aatactcgtg atcggatcat
     9001 cgtcggtctg tgtggtgtgc tgctgggact gctggttctt gtttccccgg ctcatgccga
     9061 cgacacgctt gtgacaaagc aaagtcacta tacggttgct gagacgatcg atcggatcgc
     9121 acgggccgtc accgacaagg gaatgcttct cttcgctcgc atcaatcatg cggacgaggc
     9181 gcggaaggtc gggttggaaa tgccccctac ggaattactg ctctttggta atcccagagg
     9241 cggcacggcg ctcatgctgg cggctcccac agtggccatc gatttgccga tgaaggcctt
     9301 ggcatggcaa gaccaggtcg gccgagtatg gctcacgtac aatgctcctg cgctcttgcc
     9361 ggtgcggcac ggattagcgg cagagttggc ggccagactc gatccggttg ggaccctgct
     9421 ggagcgggcg gtggagtagt ggcgggattg ttgagccgtg cgttggaaaa agagttgaag
     9481 aggattaaga tgggccgcat tgtcgctgaa tcatggagtg atgctgaagt tccagagcat
     9541 ggattgatcg gaaggatctc agatgagggc gtgaggaggg tggaagcgtt gaatgctgtg
     9601 agtcgaacga ggggagtgac gatgaggtca cagacaatcg ccgtgggcag ggtgtccttg
     9661 ttgctggtgc tgtgtgggat cggtcttctg ccgaccgtat ctgcgctggg tgccaatccg
     9721 gagtccgtgc cattgtccga tatgcgacac attaaaaagt tcgtggcggt tgaagtgcaa
     9781 acccagggaa cggctgagaa gcttggcatc aatggagcgg agttgaccga tgtgacgagg
     9841 gcgacgctgc tcaataaggt gccggggatt gcgctcgaat cgtccagtgg tccttcttcc
     9901 gacgctcccg agcggctcaa ccagcttgga ttttttacct gcgaggtctg gacggtgggc
     9961 gaacagtata ttgccgccta tcacgtagac tgtaacgcgg ggtcgtacac tgcacaaaaa
    10021 acgccgggga gcctgtggaa tcaagcgatt ctcgggtacg ggccgaggga cgaagtctcc
    10081 gatgccgtcc gaaaaggggt gcgtgcgatg gtagagctct ttgcaaacac ctttgctaat
    10141 gcccgtatgg acgccggccg gtagggccgg cgtgtgcgca ggcccagtct taccccatcg
    10201 atgcgaacga tggagcgcat ggcgtctccc tgtgcgaccg ttctccttct ctccgacgat
    10261 cctgcgtgtc ctgcactcat tcgcacctct ggaccagccc ccaaacccta ggttgaatcc
    10321 tctgaagaat ctcttttttc ttgtctgcac ttggatgcga catagaataa gggggctatg
    10381 gctgatgcat cgtcaggcca gcgaatctct cacccctatg gttggctgct actcgggctg
    10441 ctgcttctgt tcttcttccg tgtggcggca cagctgattc aagcctactc gccggttacc
    10501 tcgctgccgc cattcgaaac ctggcagagc ggagcgcttc cgtatccgct gctcgtttcg
    10561 ctccaattga tcattcttgt cgcttgcggt cgtgtcgtgt ggagttttct gtccgggacc
    10621 gtggtccctt cagggaaaaa gggccggctg ctcctggtgc tggggtgggt ctacttcgtc
    10681 gtgatgggga ttcggctgtt gattgggctc accgtcgcac cgaatcattt ttggttcggc
    10741 gccattgtac cgacgttatt tcatctggtg ctggctagtt tcctgctcgt cagtggctcc
    10801 tttcatacgg caagcggatc ctgttcggtc gcaccagtct cggaaggtgc ggcgtgatcc
    10861 ttctgcttcg atatggggcc tatccctcaa tcttgctggc ctgcgtaatc ggcaatcttg
    10921 tgctgtttca catgggcacg ggggtgctgc cggcgaccta tctgccggtc acgctcggga
    10981 cgttgctgat catggcgctg gaggcgacgt tgccctatcg atctgactgg cggccgtcgc
    11041 gggttgaggt cgttcaggat tcgacctttc tggcactcgt gcatgtcatc ctgccgaagg
    11101 ccttagcggc ggcgtcggtg gttgtgatct ttgatgttct ccacggacgg ggttggggta
    11161 tgtctgtgtg gtggccgcat gactggccgg ttccgacgca gacgatgttg atggtgctga
    11221 tcgtcgatgg cctgcggtat tggctgcatc ggctcagcca tgagtgggag ccgctctggc
    11281 gcgttcacgc ggtgcatcat gcgccgcagc ggctctatac gctcaacgtg ggacgatttc
    11341 atcccatcga taaggggttg caatttgtct tcgatgcgct gccgttcctc gcgcttggcg
    11401 ttcgggaaga cgtgttgagc ctgtacttcg tgtggtacgc cgtgaacggg ttctttcagc
    11461 actccaacgt ggatgtgcga ctgggatggc tgaattatgt ggtcagtgga ccggaactgc
    11521 atcgatggca tcattccatg cagaaggagg agtcgaatca caattacggc aatcatttga
    11581 ttgtctggga tgttgtgttc aggtcccgtt acctgccagc tgacaggtcg gtgggtgcgc
    11641 taggcttggt gaatcggcag taccctcgtg gtttcctgga tcaaatggct gcgccattta
    11701 cccccggcct tgataagcag acagtgtgag aaccgggatg tcgagatttc tccatgatcc
    11761 tgccttgtcg ctctcactga cccttacgaa gtatcgcgat tggaatccgt ttattgccca
    11821 tacccattgc ccggagctgg ctcagcaggc tgcgcttcgg gatattctgg cgcgacaagc
    11881 cggcacggcc tttgggcgca aacatcggtt cgggtccttg ctcaggtacg aggagtttgc
    11941 acgcgaggtg ccggtgtgta cgtacgaaga cctgcggcct gccattgagg tgcaggaaaa
    12001 gaacagggag ccgcttctta cctccggcca gcccatcctc tttacgcaaa ctagcgggac
    12061 gacgggagtg ccgaagaaca tcccgatcct cccccaaacg gtcgaagcga tacgccgtta
    12121 tcagggcctg tttgcctatg cgcaatggca gggagtgccg gccatctacc aaggtcgcgt
    12181 gctggtgatc agcggacaaa ccatcgaggg ccacttgcct ggcggcaccc cgttcggatc
    12241 gatgtcgggc ttgatgttcg attgtttgcc agccgccatt cggagaaaaa gcatacttcg
    12301 gggcggcgaa tccgcagcgt cggattaccg gcagcgctat ttcaatatcg cagtccgagc
    12361 gctggcggat ccctctcttt cagtgctggc cacacctaat ccgtcgacga ttctgaaatt
    12421 gctggaggtg attcgcgggg agtatgcgct actgctggag gcgttgtccg gcgagacgcg
    12481 agggggttgt ctgccgccat ttgagaagct tccaatcagt tcaacgagat tgtcacagct
    12541 tcgggtgttc atcgggcaag aggaggccct ggattgtgcc acgctctggc caaacttacg
    12601 ggccgtggtg acgtggactg gtggaaattg tgcggtactg atacccaaac tagggttgct
    12661 gttgtcgcca cagaccagga ttattgaaat gggctatctc tcgagcgaat gtttggggac
    12721 ggtgaatgtg gacgtgctca acaaccgatg tgtgccgacc ctagcggata atttgtttga
    12781 gtttgtggag gttggagacg aagcatcaga tattgaaccg gtcctgctcc atcagctgca
    12841 ggcgggccgg aaatacaccg tgattgtgac aaccaggaac ggcctgtacc ggtatgcgat
    12901 gaacgatatc attgaagtga cggggtactt caatcggacg cccacgatcc gctttgtcca
    12961 aaaagggaag ggtgtgacca atatcacggg ggaaaagctc tatgaacatc aggtcaccga
    13021 ggccgtgggg caggctctga tcgcgcgtgg gctgagctcg gaattttttg tcatgttggc
    13081 cgacgtcgag aattctcgct acaccctgtg tctagaacat gcctggtcac tgggtgatct
    13141 gggaatcctc gtggaggagc ggctctcgtc gatgaatatc gaattcaagg ccaagcgtgc
    13201 aagtgggcgg ctgcagccga ttcacgtcct ccagttgcgt cctggaaccg gggatgtcta
    13261 tcggcaacat tgcgtcagcc aggggcagcg tgaggcacag ttcaagctca ttcgcctcca
    13321 atacgcccat gattccacct tcgattttga gcggcatggg gaggtgatga cgtgatgcgc
    13381 atgcgtagct tgaccatgac atccttagag atccccttcc ggcaggcctt tacgcatgcc
    13441 tcggctacgc gaaccacgac cgaggcggtg ctggttcgcg ccgaatctgc tcgtggattg
    13501 gtggggatgg gagaaggttg tccgcggcat tacgtaacgg gagaaaccgt ggctagcgcc
    13561 caggagtttt tccgcagcca ccgggccgaa tggatgatat gttcatcgat gaatgatctg
    13621 cagacgtggg gaaccgcgca tgctgatctg atcgaccgca atccggcggc ctggtgcgcg
    13681 gttgaaacgg cctgcctgga tttattggga aaagaactgg aacagccgat tgaagtggtg
    13741 cttggcggca cgccgttgtc cgggccattt cggtactcgg cggtactcgg cggggaaaag
    13801 ttttcctcgt tcgagaaaca gctgcgacag tatcttgccg cagggtttat cgatttcaag
    13861 ctcaaggtca tgggcgatct ggaggccgat cgtgagcgtg tagcggcgct cacggctgcg
    13921 ggcacaccag gccttcgcgt gcggcttgat gccaacaatc gctggcaccg cgtggatgaa
    13981 gcctgcgagt atattcagag cttacagggc tcatttaccg ctctggaaga gccgctgcgg
    14041 gtcggcgatt acgaggggtg tcgggcactc tcccggcact gtggcgtgcc gattattctg
    14101 gacgagagtt ttgtgaaggt gggtcaattt ccgttgattg aaggcgagcc ttccttgtgg
    14161 attctaaata ttcgcgtctc caagatgggc ggtctgctaa gagcatgcac ggtcgccgcg
    14221 cgtgcgaagg cggcaggtat tccgatcgtt gttggcgcgc aagtagggga gactagcatc
    14281 ctcaccagag tggcgctggt tgtggcggac cggtatcgcg acatcctgat cgcgcaggag
    14341 ggggcagtgg ggacgcattt gcttgaatat gatctgtgtg agccgccgct tatgttcggc
    14401 agaggcggat cccttacgga tatcttcttt cgcgaccgcc ctggtcttgg gctttcgttt
    14461 gcgccgtaag atcgccgtct tcaggagtcg ggtgagggtg aggttgtata aatacgtagc
    14521 ctgacaggtg cccgatcaga ggctttccac gtccgcaaca atcgtgtgta gcccgctcga
    14581 tccggccggc tgcggtcgaa tgagttcggc gatctgaggg tgccctttgc tatcgatcgc
    14641 gcgaacggcg acctggtact tgccccgttt cgaggggcgc caagtgtagt tccaaatgac
    14701 ccatgaatag ggcgacatcg gcgtttcaat ctcgcaggaa tcccacgttc tcgtggcatc
    14761 caggctgatt tccactcggc tgatggagtg tgggccgcca aaggcaattc cgcggaatcg
    14821 ctgctcgggg ccgcgtagcg tctggtagtg tccgggggag tcaatgcgtg aaaatgtctt
    14881 gatggtggcg tcatcggtcc acccttttcg ttgccaataa cctcgataat ccccggcgta
    14941 ggcctcgatt tccacaatcc atttcacatt cttgatgccg tagagtccag gtaccaatag
    15001 gcgcagcgga aagccgtgtt ccttcggtaa cttctcgccg ttcatgagaa acgcgagcat
    15061 cacatcatcc tgcatggcgc gcgtcagcgg aatgctatcg tcgtaggcgt ccgctccgcg
    15121 gaagacaatg tcacgcgtgg tttcttcatc gatctccgct tcctgtagga gtttcttgag
    15181 ggaaatcccc ctccagcgtg cgttcccgag gctatcgcct ccgggtaatg tatcgatgca
    15241 catgagggta gagatctgct cgaagttatt gcggtttaat agctctcgcc agccaagaga
    15301 cagaggtttc ttcacttctc ccttcaccga gaggcgccat tcgtcttgtc gaagctcgcg
    15361 agagacgtta aaaggggagt ctgagtaatt caccacgtag aattttttgt tttctgtgaa
    15421 gtaatttgtc tcacgcggag gcatggcgaa catgcgaccg aataaagatc cgaccgcatc
    15481 gcagccaccg gtgagaccag caacagccgc aattccagtg agctttaaga gatttcgtcg
    15541 tttcatcgag agccaatttt ccatggaaat actctgcgac aataaaaaga tcttttcaag
    15601 gcttgactcc agaaacccag attggtataa ggatgcacgt ttgatccagc ctagtggccg
    15661 atgcggttgt aagacctgcg acggattctc aggaacccca gatcaaaaaa ataaatcctg
    15721 gccctcttga caaggtcagg cgcgcatagt aaagtgcgcg gctcgcgtgg ggaacgacaa
    15781 agcgttccga agcgtgaaac ggttctttga caactgaata gagcgagaaa tgagtaaggt
    15841 aaggtgtatt gaagaggtgg ccctcgggtc caattcaagt agaaccttta tgagagtttg
    15901 atcctggctc agaacgaacg ctggcggcgc gcctaataca tgcaagtcga gcgagaaggt
    15961 gtagcaatac acttgtaaag cggcgaacgg gtgaggaata catgggtaat ctaccatcga
    16021 gtggggaata accagccgaa aggttggcta ataccgcgta cgcctccgga tcttcgggtt
    16081 cggagggaaa gccacactgt gagtgcggcg ctctttgatg agctcatgtc ctatcagctt
    16141 gttggtaggg taacggccta ccaaggcttt gacgggtagc tggtctgaga ggacgatcag
    16201 ccacactggc actgcgacac gggccagact cctacgggag gcagcagtaa ggaatattgc
    16261 gcaatgggcg aaagcctgac gcagcgacgc cgcgtggggg atgaaggtct tcggattgta
    16321 aacccctttc gggagggaag atggagcgag caatcgttcg gacggtacct ccagaagcag
    16381 ccacggccaa cttcgtgcca gcagccgcgg taatacgaag gtggcaagcg ttgttcggat
    16441 tcactgggcg tacagggtgt gtaggcggtt tggtaagcct tctgttaaag cttcgggccc
    16501 aacccggaaa gcgcagaggg tactgccagg ctagagggtg ggagaggagc gcggaattcc
    16561 cggtgtagcg gtgaaatgcg tagagatcgg gaggaaggcc ggtggcgaag gcggcgctct
    16621 ggaacatacc tgacgctgag acacgaaagc gtggggagca aacaggatta gataccctgg
    16681 tagtccacgc cctaaactat ggatactaag tgtcggcggg ttaccgccgg tgccgcagct
    16741 aacgcattaa gtatcccgcc tgggaagtac ggccgcaagg ttgaaactca aaggaattga
    16801 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg atgatacgcg aggaacctta
    16861 cctaggctag aatgtgagtg aatatatcgg aaacggtata gtccttcggg acacgaaaca
    16921 aggtgctgca tggttgtcgt cagctcgtgc cgtgaggtgt tgggttaagt cccgcaacga
    16981 gcgcaacccc tgctttcagt tgctaccggg tcatgccgag cactctgaaa ggactgccca
    17041 ggataacggg gaggaaggtg gggatgacgt caagtcagca tggcctttat gcctggggcc
    17101 acacacgtgc tacaatggcc ggtacaaagc gctgcaaacc tgcgaggggg agccaatcgc
    17161 aaaaaaccgg cctcagttca gattgaggtc tgcaactcga cctcatgaag gcggaatcgc
    17221 tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta cacaccgccc
    17281 gtcacaccac gaaagcctgt tgtacctgaa gtcgcccaag ccaaccgcaa ggaggcaggc
    17341 gcccacggta tggccggtga ttggggtgaa gtcgtaacaa ggtagccgta ggggaacctg
    17401 cggctggatc acctcctttc taaggagtac cactccttct gaaaaaaact aggatcttga
    17461 gggtcacctt ttcaatacac tagacacggt cccgaaccgc atcatccagg tgagtattat
    17521 cactgttggc tcacgagatg tagtgccaca cgagagttta tgattgtctt ggggtggctc
    17581 agcagtcggc tgggcctgta gctcagctgg ctagagcacg cgcttgataa gcgcggggtc
    17641 gatagttcga tcctatccag gcccaccaac tgagcctcga gtgtgagtat ctccctcggc
    17701 ggctcaaaat tgacaatcat tcatttgtga gcggggctgt agctcagttg ggagagcacc
    17761 tgctttgcaa gcagggggtc gctggttcga ttccagtcag ctccaccaag ggtttgtact
    17821 gcattcattt ctcgctctat tcggtcgtgg gtggcacgcg atgtcatcca agttctttga
    17881 aaatttaata ggtctgagaa gaccatgtgt atcaggagca aagagaattg ttaagctact
    17941 aagggtgtac ggtgaatgcc ctggcatcag aaggcgatga aggacgtagc tagctgcgat
    18001 aagccccggg gagccgcaag caggccgtga tccgaggatg tccgaatggg gaaacccagg
    18061 cgatgaatgc cgcctatcct ctgctgaata cataggcagt gagaagccca cgcagggaag
    18121 tgaaacatct cagtacctgc aggaaaaaaa atcaatcgag attcccccag tagtggcgag
    18181 cgaaaaggga acagcctaaa ctatgccgat gtcaagcccg tgtgcgttgt cggtgtagtg
    18241 ttgtaggcct ccgttggacg gtgatacgga accgtcggcg agttacaaat taggatcgta
    18301 acagaatagt ctggaaaggc tgaccataga aggtgacagt cctgtatgtg aaacgatcct
    18361 aactcgttga atggaggtcc tgagtaccac ggggcccgag aaaccttgtg ggaatctggg
    18421 acgaccacgt tctaaggcta aatactctct gatgaccgat agtgaaccag taccgtgagg
    18481 gaaaggtgaa aagaaccccg gtgaggggag tgaaatagaa cctgaaaccg tacacctaca
    18541 agcagcgaaa gggctatgcc cgcaagggaa tgcctgatcg tgtgcctttt gcttaatgag
    18601 cctgcgagtt atttagcgta gcaaggttaa ggcgaagagc cggagccgta gcgaaagcga
    18661 gtccgaattg ggcattgagt tgcgctgaat agacccgaag cgaagtgatc tacccatggc
    18721 caaggcgaat ccgccgtaac aggcggagga ggcctgaact gatgttagtt gcaaatggct
    18781 cggatgagct gtgggtaggg gtgaaaggct aatcaaactt cgtgattgct ggttctcccc
    18841 gaaataacta gagggttagc ctcgtttcag ccgtgacgga ggtagagcac tgaatgagct
    18901 aggggcgttc caacgctacc gaactcaatc aaactccgaa tgccgttact ggacgaacgg
    18961 gagttagact acgagtgcta agatccgtgg tcaaaaggga aatagcccag accgtcagct
    19021 aaggtcccta atcgtgagct aagtggaaaa ggttgtaaga tcgctcagac agccaggagg
    19081 ttggcttaga agcagccatc ctttaaagag tgcgtaatag ctcactggtc gagcgatttt
    19141 gcgccgaaaa tgtaacgggg ctcaagttca caaccgaagc tacggacttg catcgcaagg
    19201 tgtgagtggt aggggagcat tctctgtgcg gtgaaggttg accgacaagg acaactggag
    19261 cgcagagaag agattatgca ggcataagta gcgataaacg atgtgagaat catcgtcccc
    19321 gtaagcctaa ggtttcctgg cctatgttcg tcaggtcagg gtgagtcgga tcctaagccg
    19381 aggtcgacag acgtaggcga tggcaaacag gtcaatattc ctgtaccacc gtattgacgt
    19441 tatcagcgaa ggggggacgc agaagggtag gcaccgccgg gtccctggaa tacccggtcc
    19501 aagcaggtag gcgggggttg taggcaaatc cgcaatcccg ttaacgccga gatgtgatgg
    19561 ggaggccgca aggccataaa cgggctgatc ccacgctgcc gagaaaagcc tcgtagcgag
    19621 tcaatatgat gtccgtaccg caaaccgaca caggtaggtg ggtggaatac accaaggggc
    19681 gtgagagaac actccctaag gaactatgca atttagcccc gtaacttcgg gagaaggggt
    19741 gccacgagta ggtaagtctg tacaaggcaa gctgaacgtg gttgcaataa agtggctcta
    19801 gcgactgttt accaaaaaca caggactctg caaacacgat agtggatgta tagggtctga
    19861 cgcctgcccg gtgctggaag gttaaccgga ggagttagcc gcaaggcaaa gctttgaagg
    19921 gaagccccag taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
    19981 gggtaagttc cgacctgcat gaatggcgta acgactggag cgctgtctca gggagtgact
    20041 cagcgaattt gttgttccgg tgaagaagcc gggtgcccgc aactagacgg aaagaccctg
    20101 tgcaccttta ctacaccttg acattgggtg ttggaaggat ctgtgtagga taggtgggag
    20161 gctgtgagac ttggccgcta ggtcaggtgg agccgacgtt gaaataccac cctgattgtt
    20221 ctgatattct aacctggccc tgtgatccag ggcagagaca gtgtctggcg ggtagtttga
    20281 ctggggcggt cgcctcccaa aaggtaacgg aggcgcccaa aggttccctc aggctggtcg
    20341 gtaatcagcc gtcgagtgta aaggcaaaag ggagcttgac tgcgagagcg acagttcgag
    20401 cagagacgaa agtcgggctt agtgatccgg tggttctgcg tggaagggcc atcgctcaac
    20461 ggataaaagg tacgccgggg ataacaggct gatctccccc aagagttcac atcgacgggg
    20521 aggtttggca cctcgatgtc gactcatcgc atcctggggc tgaagctggt cccaagggtt
    20581 aggctgttcg cctattaaag cggtacgaga gttgggttca gaacgtcgtg agacagttcg
    20641 gtccctatct gttgtgggcg gaggagagtt gagaggggcg ttcactagta cgagaggact
    20701 gtgaaggatg aacctctggt gtgccggttg tcgtgccaac ggcagtgccg ggtagctatg
    20761 ttcagttggg ataaccgctg aaagcatcta agcgggaagc ctgcctcaag ataagctctc
    20821 ccggggcgca agccccctaa aggacacttg tagactacga gtttgatagg ctgggtgtgg
    20881 aaggctcgta agggctgtag ctaaccagta ctaatcgtcc gtgcggctta acatcctttg
    20941 ctcctgatag acgtggtctt aataagaaca aatgaatgac gaactatgtc ttcgtcgatc
    21001 agggacgagt ccaatatgat cgcctagaat aggaactcac tggtttgatt cttcagaagt
    21061 tcccggtggc cttgccggag gggtcacacc cgttcccata ccgaacacgg aagttaagcc
    21121 ctccaaggcc gatgatactg ctgccgtgag gcagtgggac agtaggacgc tgccgggtta
    21181 caaaaagagc ctgctcgaga aatcgggcag gcttttttat ttgtcattga atctcagaag
    21241 gtctaattga ttaatgcagt cgcccatggg tgtttgccga attgatttag cgcttatcag
    21301 agctcaatgg caattgaagt gtacagtgta cagcgctttt catttgaaat tcattgctga
    21361 ctaaagagtc cctttctatg ctgccaccta ttccatccag ctggtaccag tttttcacgg
    21421 aacagataaa tggcaacaac tttcgagctc tcgaaacgtt tctcgatcgg gaagcggagg
    21481 caggtgaaac aatcttgcct ccctcgcagg atatttttgg ggccttggag gcgacgccat
    21541 acgaacaggt caatgttctg cttcttggtc aggatcccta ccatacacca ggaatggcgc
    21601 acggactctg tttttcggta cggcctgacg tcccggtttt accccgctcg ctcagaaata
    21661 tctatcgtga acttcgggag gatgtcggct gctctgttcc aaacaacggc tgcctggagc
    21721 cctgggccag gcagggagtg ctgatgttga atgccgttct gacggttcgc gcgcatgcgg
    21781 ccaattcaca tcgggggcgt ggctgggaag ctgtgactga tcgtatcttt gcggtcctga
    21841 atgcaaaacc aagtcgtgtg gtattcctgc tctggggcgc ggaggcgaag aaaaaacaat
    21901 cactgatcac aaatgcgcag catgtggtga tttcctgcgc tcacccctcg cccttgtccg
    21961 cgagaaagtt cttcggttca cggtgtttct cgcaggtcaa tcgagccctc actgaggtgc
    22021 accttaaacc gatcgattgg cagattcccg atctctgaat cggagtcggg cggcgcggta
    22081 gcagtgatcg gtgctcacac ctaaatgatt tgacttgcgg taagtcgttt tttatactac
    22141 agcaattctt ttagttctta ctcaatcatc tgcgcaggag agtggccgcc gggaatgagg
    22201 attcgtctgg gtctgggtct cggttttagt actctagttg tagcaggtac gttgttggcg
    22261 gccgagggcg accttgccgg actgcggacg ccaacgtcta ttggatcggc gcccgtcgcg
    22321 tggcagtatg atggcgacca agggccctcc cattgggggg aaatcgctcc caccaccgcc
    22381 tcctgtgaaa aaggcactca ccagtcgccc attaatattc ggaccactcc ccatcaccag
    22441 agccatgacg ggctcttagt tcattacact gccgctcccg gtcacgttgt cacttctcac
    22501 cacaccattg aagtggattt tcaatcaggc gagtcgcttg aagtccttgg acgaacgtat
    22561 acgctcaaag agtttcattt ccacgagccc agcgaacatc agctgaacgg ccgcacttat
    22621 ccgatggagg cgcacctcgt gcatcgagat gagacgggtc atctggtggt cctggcggtg
    22681 ctgatggatt tgggtaatga atcggcctct ctgtcagccg tgtgggatcg gattccgagc
    22741 gagaagcagg atgaggtgcg ggacttgctg attaacccgc aggatctgct gccgaaagac
    22801 ttgcaccatt acgcctatga tggctcactg accacgcccc cttgtacgga aggggtgcac
    22861 tggattgtgc tgaaggaacc cacctccatt acctctgccc atattgaacg gttcgtgtcg
    22921 ctgattggcc acaacgctcg accggttcaa tcgttgaatg agcgtgagat tgacgaagaa
    22981 taagcttttt cgttcagaca acgatcagat tttcagagcc acccaccgtt gattcgggcg
    23041 ttcccgcgac caccagtccc agctttactt cgagtgtctt tcgagcaatt gtgggcggtc
    23101 acgtgaacgt gtgtcgacca gcctcagcca cttagtggat gccatcctcc tgtggaggct
    23161 gaggtcctgc gaccatacct gccaggtcgg ggtcatcttg accttgcgat cttggccctt
    23221 ctgtttttcg ttttaggcgg tgagcctctt tctgcgtgcg gcgatcacgt tgggtctgtt
    23281 cccgttgccg ttttttagaa gtcgtatggc tcatggtcac ctccgtgggt cgcatgggag
    23341 cgacgtatat caggcggatg gcacttgagg gggcgtcgtt gctggcacgg cgctcgatct
    23401 gccggctttg atgaatcgat cttcggcgag cacgccgtgt agttgtgccg tgatcgagcg
    23461 acggtacggt tccttctgcc ggatcatgat cacgagattg tccccatcca caaggatgct
    23521 gaggtcgtct tcagtgacta gttcccggga cgagccaccg atgttcaatc ggtactgtgt
    23581 ttttccatcg ggattccgat tcggtccgac cacgatctca gtcaatccgt cgatgtggcc
    23641 gtcatacccc ttatgccggt gcctgacctt ggtgccgttt ggaatgcgta cccatcgtgc
    23701 caccggcgat gtctgactgt cttgcccgtt gctcatagcg cgtgtgtctc cttgttatcg
    23761 tacccgtacg tagccgccag ggtgctcgtg attccgtgag cgcacaattg catcgcggta
    23821 cacggctggg ctgacgactg cgaatttcag aatgaaacga gcggagtatc gcggacggaa
    23881 gggcggttga atcgatcttg ctaatctacg gacacagggt cgataggaag caaagttaag
    23941 cggctgagga gtcagcgatc catgcagagt gtatcacatt tgttcatgcc gcatcagcca
    24001 cgccttagga tggaggtgct tggttattgt cgaggttccg caatccccat atgcaggtgt
    24061 ggtcatgcgc gatgctgttg atttggagca atcggcacac gaatatggtg caaaacgacc
    24121 gatcgctgat aataattatt gccgcataga atcatgctct tgcattgaga tcggcgctcc
    24181 ggcacgttga caccactctc gcagccctat agggtgaacc catgacgtac taagccacgc
    24241 cggctttctc ctttcgcgcg cactttctct cccccctcac tccctgcgcg tgcgagtctc
    24301 aagtcttcgc cgcttcgacg ccacagaaca agagtccagc gatggtaggc ccgcagtgat
    24361 cggtcattga ccgatcagtc gatctactca ggatggagtc ttttgcctac ggcagattcg
    24421 cgcaacgagg gaggttggta tgtatcaaat tttgacactc atcggaatca tggtctgacg
    24481 ctactgcgta gacagaggat ttgagttgcg agtgctcttg aggtctcctg ccaatcggca
    24541 gggatctgat gacagatgga ggataccatg atggcgcaga tggtactgtt ctgggccgca
    24601 cacaatttgt ttgcggtcgt gacgatttgc ttcggtggag ccatgctgag cgtcatgctg
    24661 tacacctatc tgcatgatta tgtgctggtg gcgaaggggc gctagcttgc tatggtaagt
    24721 tgcacggttg cctattgggg aggtttcatc ctatgcgtat tcatccaaca atcaccacga
    24781 gagtattgag tctttttctg gcaaccgctt ggttgcttgc cgtctcactg accgtccagg
    24841 cctcggaaca gaaacccgtt gcgaccgtcg cgccggtcaa gtccgttgcg gtagcttccg
    24901 gcgccgtcga ggatactctg cctatgtgcc tgacaagaat tcctcaggag gcgacaccag
    24961 ggcagcggtt gatcgctgaa cagagttgcc agcgagacga atccgctcga gcatccatgc
    25021 agaatgttcc tggacggtag ccagatcctc ctccataact gcctgcgtat ggatgtggct
    25081 gggtgcggtg tgccggccgt gcacggcaca gatagtagcg gccgtttttt catggtgccg
    25141 ggctatgttg aaggctgagc aatgcaggaa ggatgacggt atgcgtatgg aacatcgagt
    25201 tcgttcaaga tccgcaggcc ggcctggtgc cgtcgaggag cgaatatcgg caagggcgag
    25261 tgagatgcct gtgggagtcg gtcggaatat gctcctcgat atcaataggg ctgagatttc
    25321 ccgactcaag tcgctgactg gaatcggcga acattatgcg aggaagatcg tggaagggcg
    25381 gccttaccac catcgagaag agttgctgac caaagagatt ttgccggaat acatctacgg
    25441 cagaattatg gatcgactgg tggcaaacca gtcttaggaa ttgagagatg agtgatgtgc
    25501 ctggcatcgg atgcctcgca gaggtggccc tgcggcatgg aataaccgga gggttctctc
    25561 tcatgattgg gacgggtggc tcgaatgacc ggaaggaacc actgcatgac ggacactagc
    25621 agaacatcac gggcggcgag aaggttctgg ctacgtcagg catcttggcg gggcatggcc
    25681 ctcgtagcag ccattagcct gctgatgggt atcgtgcagg ccacggccgc atggtccgag
    25741 cagggtccca ctgaggccgt gaaggggacc gtctccgagc tgctctcgat cctcaaggaa
    25801 ctcaaagatc cgactcgatc tgaggcgcgg cgttgggaaa tcgaacaggt cattcggcat
    25861 catgtgcatt acgaagacat ggccaagcgg tctctgggag catcctgggg gcaactgagc
    25921 gatgcggctc gccgggagta tgtcgggctc tttgtccagc tgcttcgcga tgcgcttgcg
    25981 aaccggatgg tggagtattc cggtgaacgg atcagctatc tttcggaaca acgggagcgc
    26041 acgttcgcgc aggtcaaaac gcggctggtc gggaacaagg tcgacacctt catcgacttt
    26101 cgtttggtta gtcaggacgg acgctggctg gtctacgacg cagtgatgga cggggggagc
    26161 ttggtcgcga gttaccatgc gcagtttgcg agcatcattc gcgatgcctc ctgtgcacag
    26221 ttgatggagc gaattaaaga gaagacgctg gtcgtgaaat tgtttgagaa gagcgggtcg
    26281 tgaggcgagg gcgcaatggc acgatcacga gcggtcacgt ccccgggaga gagcgcaatg
    26341 agatgtccgc gttgttatgg tccgatgatg atgatcaaaa tgaaggacac cgtcagtggt
    26401 acgctggcgt acggctgggg ctgccggcaa tgtggtgccg tcatcgattc actgatcgat
    26461 attcaccgcc agtggcatct ggaaccccgt gcgacttggg cgcatctggc tgtcgccgcc
    26521 tgctaggtca tgcaattact tccccctgtt ttcgtaggtt cgcctgaccg gttggggcgc
    26581 ccgatgaccg gaattcattt caagtctcag gaagagtgaa ggagtgtgtc atgttgcacg
    26641 ccgcgttgat tgctctcgcg cttggcctcg tggtttgggg gctcaactgg accgggctcg
    26701 ccgagatgcc gacccaggtc tattgggtct ttaccgtggg gggaatgctg ttgatgctga
    26761 tccacgttgc gacaaggcga ccggcccgcg tgtggtaggg ggacccgtca ctatgcgact
    26821 ccgcgatgca tgcatcataa gcgccgtgct gggaattggt gcgttctgct tcagtgaggc
    26881 cgaagctgcg aaggagtggg cgccgcagga ggttgcggca attctcgcgc agtcgtatgc
    26941 gcagctgctc atgcgaccga tggggacgga gccaagttcg cttcagaaga acgaggcgga
    27001 ccaacaacgg tatatggtgc agtccggcta tgcgtcgcag ctggcaagag gggagaaaag
    27061 tgtgaagcag attgtcgagg cgatcgccct atcatcggaa tttaacgcca agtggataac
    27121 gccccacatg ggaactgcgc agaccggatc ggcgccggga gtatcgcccg tggcggcccc
    27181 ggaaaaggcc atcgatagcc tgtactgtgc gttgttaggc cggcgtgtgg atagtgcgtc
    27241 gttggccagc gctcgcaagg atttggtgag ttccggcttt gaacgggtga tccgtgacgt
    27301 gatcgacggg aaggaatacc gagaccggtt cggtgacacc aaagtgcctc aggtgagcag
    27361 tgagaaacag gcggcagtcg gttgtccgca agtggctctg tagcagcggt tccgcgcagc
    27421 aggctcagga ttcactgcgc aggtggcttg acgggacggc aataacctca gtgaaggtgg
    27481 gagagaagca cggatgagta tgacgccgga agaacgcatc gcaaatatga caaaggcgat
    27541 caatcaagcc atcaaggtga tgggggatcg tttcgggtcg acagaggaag atgtggcgcg
    27601 agctatccaa ggcctgaagg cctcaatgca gacggcaccg caggagaagc cagtgccagt
    27661 cggggccgcc actcaggcct aagagctgcg gtgaagcctc accgggccca ctgtctgatc
    27721 ggtgagggag ccatgtggtt cggcgatgct ccgatgtctc tcgtcgttgg gagcataggt
    27781 ttcatctgta ctcccttgct gtctcccggc acgagtcaac ctacggacta ggtctcaata
    27841 tcgcttgact ccgatcgcca tagcgcttag cgtgaaggca tgacgtcttt agctccgccg
    27901 actgttctct ccctcgcgct tcgagcctcc atcccgcgcg actgatcgtc cggtctccgc
    27961 cgcttcttac gccagagtgc cctgcactga ccgatgatgc cgtcttcgtg aacggagcga
    28021 ttgtcgcttc cggcgaggag cggtgtcgtg accagcagtt ccatccgaca ccattatccg
    28081 tttcatatag cagtacgagg gagcaccatt atgattacca tcacggcaac ggctgaagag
    28141 aaaattcgcg agttgatgct ggaagaaaaa gatacgctcg ggctgcgtgt atatgtaaaa
    28201 ggcgggggct gccacggcta tcaatatgga atggcgttcg agtcgaagat gagcgacgac
    28261 gataccgtga ttgaaaaggg tgatgtgaaa gtgatcatgg attcgcaaag cgctccgctg
    28321 ttgagtggct gcgaagtcga ttatgttgac agcgtgcagg gctcgggctt tgcgatcaag
    28381 aatccacaag ccaagacgac ctgcggatgc gggagctcat tcagcgccta atcccggcgc
    28441 gcgtcgctgt ctttgcatga tcgtcactgt gactgaagga ggtccgccat ggaacccaca
    28501 tccgtcattg gaacaaagaa taagaaaggc caggtcttca ccgacccccg catcatgatg
    28561 cgggaggcgc ctcgtacgcc atcgttttat accggcagcg aagtcattaa ggaagccatc
    28621 cggcgcgcca gtatcgacgt gatgatcgcc tatccgatca cgccacaaag cgaggccgct
    28681 gcgttgatcg gcgagctgtt tgccgagggg tatatcggag actatttccg cggggaaagt
    28741 gaatttgccg tcatgtcgca atgtgcaggg gccgccttcg gtggggctcg tgtcttcacg
    28801 acgacggcag gacccggcac gatgcgggcg atggaaaact ttcccatgtg ggcgggtgcc
    28861 cgcctgccga tccaatgtat cgtgacctgc cggggaatta attccccatt gtcgattcaa
    28921 ccggacacca tcgagatttc ctatttgatg cagacaggca tgcttgtttg gcatgcggag
    28981 acggcgcaag atttctatga ttggattctc atgggctata tggtgtcgga agagcccgag
    29041 gtgcatttac cattggcgct ctgctgcgat ggattctttg tgacacacac caaagacgtt
    29101 gtcgatctga cgccgacaga tatgtgtctg ccgccctatg atccgtatcg gtcgccggtc
    29161 ccctgcatgg atatggaatg tccgcctgtc cggatgatgc gtgatccgtt catcatgaaa
    29221 agcaactaca tcagttatgc cacgcacgcg agctggcagc aggaagtctg ggctgccgca
    29281 gaacggtcac ggaagcacac gatcgcctgg ttgaacgggc tgatcgaaac cgaagacgtt
    29341 gacgccgatg tgctcattgt cacatccggt accgccgtgt ctcaggggcg ggaagccatt
    29401 cgtctcctgg cggatgaagg tattcgcgtg ggacttgtga agatcaaaac gctccggccc
    29461 tggccggaag aggaaattaa ggaagccacc aagcacgcca cgcacatcat tgttccggaa
    29521 ttcaatgtca tcggctggat ggcgaaagag atcaaggcca ccattccaag aagtgagcgt
    29581 gtcatcgccg gtccgcgagt gtgtggcggg atgaccatgc cgccggagat catcgtggaa
    29641 gagattaagc gtgcgcttgg cgtgcgttct ggcacgctcg ccggtcgcgg gtgaagcgca
    29701 gagggctaga caacgagaac ggtttcgata cggtgtgcag cacattgacg ctcagcaggg
    29761 ctgagaggag atagatatga gcaaagaacg catcaagatt tcgccggatc tgtatgacat
    29821 catgccgtcg gattaccagg acctggtcag cagcgccacc tacggcaagg aagaccgggg
    29881 atggaaggac atcggtacct ccaaagaact gatcgagcag cattcactct gtgccggatg
    29941 tcctgaatcc atggcgtttc gatatatttt ggcgtcgctt cccaaccccg aagacacggt
    30001 catggtcggc tcgaccggct gtacgagtct cgtgtttccg atggtggccg tccacaacat
    30061 tcattccctg ttcggcaacc agaatgccat cgcgtcgggg ctcaagcgtg cgttgagcgt
    30121 ccgctttcct gatcgtatta aggatgtggt ggtgctggcg ggagatggcg cgacggtgga
    30181 catcggtctg gacatgacgc tgcaggcctg gttccggcag gaaaaattca cgacgatctg
    30241 cttcgacaac gaactctacg ccaataccgg cgggcaggag agcggtctca tgcagaaggg
    30301 gttcgttgcc aaaatggcgc ccgtcggaaa acttttcgat aaagtacgtt tgcctgaaat
    30361 tgcccgggag tccggctgtc actacgtcgt caactgtacg gtgagcaaac cgtcgctggt
    30421 tgaaaaggtc gtccgcaacg cggttctgat cgcacgggaa atcggcccga cctatcttca
    30481 gctctatacg ccctgcattc tggaaatcgg caagaacagc atggagggtc tgcaggaaat
    30541 gcgggactcc gagaagccta ctgaacggtt tgccttcaag gaatatatca gcgagccggc
    30601 gaaacagctt ctggcggagc gtgatgccaa ggccaaagaa aagaaagccg ctgcaaagca
    30661 actggtttcg taagcggtag gattgtcacc gccgcttccg cggctatgag agagcgaggt
    30721 ttcagatgag tcttgattat gtaaagttca cacccggttt tgaacagttc atgccgaaag
    30781 aatatcggga tatggtcgag catggaccat tcggaaaaaa gacgacggtg tcgcagatgg
    30841 gcagcttcaa ggaaatcctg gaggagcacc cgatgtgtgc cggttgcgcc atgaccctgt
    30901 tcattcggtt ggcgatcatt gcttttccga atcccgaaga taccattacc gtcgggacgg
    30961 cgggttgtgg aagattggcc atttcgcaag cggcgattcc gttcgtgtac gggaattacg
    31021 gcgaccagaa cggtgtcgcc agcggtttat cgcggggact ccggctccgt ttcggcgaca
    31081 agccgaagga tgtggtcgta atcgcgggtg atggaggaac cgctgacatc gggtttcagc
    31141 aggtgctgca ttcctggttt cggaaggaac ggttcacgac catcatgctg gacaacgaag
    31201 tgtacggcaa caccggcggt caggaaagcg ggatgacgac cagaggcgcc gtgctgaaaa
    31261 tggctccgct cggaaagaag ttcgagaaga tggatatggt cacgatggcc aaagtcgccg
    31321 gctgtgccta tatcgccacg gttgtgccga acaatccgcg acgggtggaa agcgtcatca
    31381 agaaggccgt gctgatcgcc agggacatcg gcccgaccta tattcaggcg tatacctcat
    31441 gcaacatcga gtatgcgatc ccgaccgaca aggtcatgga agatgccaag accgttgagg
    31501 acgatcgtta caaatttacc gagtatgtca gcgacgaagc gaaagcgtat cttgccgagc
    31561 gctatggcta caaggaattc atgccgaagg ccgtgcctgc ttccgtcacg acggcctgag
    31621 gagtcgtaaa ggataagcag ttattctcca gactcgctct ggcgggcagg agctgaacct
    31681 cctgcccgcc cagttcgcta ggcatggggc gcagttgact gcagttgatt ctgatccatc
    31741 acactgcccc acacgagcgg ttctctcgag tcttgccaga aaataattaa gccgcgtgga
    31801 atcaagggct tgcctcatcg accttcgcag cctccattga cacagccggt cgtgcactgt
    31861 agggtggggc atgacgtctt gagccacgcc gactgtccca tcttgcgcgc ctccttccat
    31921 ccttcgcgcg tctgattccc cggtcttcgc cgcttctacg ccagagtttc catctcagac
    31981 caccaggtct cggcatgcgt ttgcgcgtgt tagccctcac aacggtgtgg cgctgtgctc
    32041 gccaccgata aaggagaatg caatgacaca ggaacagttc caacagttct ggctgcagct
    32101 caaagctccc ctcaaggcca actgggacaa gatcactgaa agcgatctcg gcgaaatcca
    32161 gggcagtctg gtgaagttcg gtgatgtgct tcagaagcgt tatggtgaag ggcacaaaga
    32221 tgaagtcagc ctctgggctg accgccggca tgcgcattgg agcggaaatt acatcggcta
    32281 taaggatcca aagccggccg tctaattcta attcggtacc tccgttcagg aagtcgattt
    32341 ctatgggtgg gtcgcgccct cgcttgcgat aagaaacaga aagagagtac ctgttatgcg
    32401 aaaaatcgtc atcaacaaga gttacgatca gttctgcctg agccacaaag cgttcttgcg
    32461 tctgcgtgag ttgggtcagc cggatgcgct caaggagacg gatctgggag cctactggcc
    32521 ctcagcggcc ggccctcagg agccgagcct caaccaatgc ggcatgttga ttccacgcga
    32581 cgacaaacgc ttggttcagg tggtggaaga attgcagggt gaggccaatg gccattgtgc
    32641 ccaactgaag gtcgtgtcga tcccggacga cgtgtcatgg gtcatcgcga aagcgaatgg
    32701 cagtgagcat gtgagcgaac agcatcggac gtggagctga gccgatgagg aacgtagcga
    32761 cgggcacgcc cgcctacggg ttggattgaa ctagggtgcc tgatattacg gggaagcgag
    32821 gcctgtctgc ccgtgcgaga ctcttacagc gtacggtgac gagtttgacc tgcctggaag
    32881 gggaggttga agacctgctt tcggaggatt aatctatcca gatctagcct gtcttctctc
    32941 aaatcgcgag cagagtctgt ccctttccac ggtggcgact ctgctcatag catgctgttc
    33001 cggtcctgcc ttttgagccc aagccagagc cgagttggtt ggtcaccccg gcggtagaaa
    33061 cacggtcgtt gtgatcgagt ctccggctgg gatcacgatt gtgttaataa gtccaggtca
    33121 gcagggtggt gaacacgttc ttgtcatagg catagacggg gatattcgaa ccgttgagca
    33181 cgccttgata ttgaagcgct accgtcacgc tacgaaacag cggcctggcg atttgaacga
    33241 aaatatcctg ctcggtatcc cggcgccggg acaactggcc ggcatcgtcg agaaaaatga
    33301 cttgcgcatg gttgtatgcg cgccagtgca cttcatagtc gactcgcaaa ctgagatcat
    33361 accagggaag agtggtttgt ccgcccagct gggcccgatt gccggaatag gcgaaactgg
    33421 atccgctggc agcttcggta tcgtgctggt agccgacacg gacaatgaac ttgtcctgtc
    33481 gaaaccggaa ggcatggaga acgccgacca tattgttgaa ggcatcgcgc gactcaggag
    33541 cgaaccggat gtcgctgttg ccaggctccg tgaagaagct tttgcgctga aagcgataga
    33601 gcagggtcgt caagttgtcc atgcggccga gggctggaag cgtgatcgcg ggcggaacga
    33661 acgtcgcagg gagggtcacg gtgtggcggg agagaaatcc cttgaggccg aggagcatgt
    33721 aatcgtagct gtactgacca ccgatctcgt agggcatcgc gccgacggtg ccgcgataga
    33781 cgcctgacaa gcttccgagg tgatcttgaa tgttgaagct tcccgcgccg ctatcggtat
    33841 ttacggtttg gtaataggaa taggtgacgg ttgattcgat cggtcccttc cgataccagg
    33901 cataatctcc ccgcgccgac gtgacaaacc caggtgattg ggtgttacgg gagcgaagca
    33961 gctctgcaac ctgctcgctg ctcttccgtg gattgacggc gacattgtcg tcataatagc
    34021 ctccgaccgc cagttgcagg cgaagccgct gggtatccgt gactcgtgtt gttgccgtga
    34081 gggcttcttg aatgcgtaca gatgcgccgg tgatcggaga agtgggttgc acccgctgtg
    34141 ccgacgccaa ttcggcctgc gcctgatctg agagtccgag tatccccagc gcgaggcctc
    34201 gatagaacat ggtcagttgc cggatgtctt gatctgcgga gcggctgacg ctcaatgccg
    34261 cctccgcgcc gttatagttc ttctgccgat agcgtaaaaa tcccacatga aatccgaggt
    34321 tctcgcgatc cggctccaat cgaaacacgt cctccaaaag gggcgcggcc ttgtcatagt
    34381 ttttcgtggc gaaatgggcg acgcccaatt ggtaggtggc ttctgggtcg gatgggcgaa
    34441 gggtgtgcag cgcagtgaga ggcgcgattg ctagctccgc ctgtccccgc gccagatgac
    34501 agagcgccag atagtagaac gctcgcgcat tgcgcggatc ggtcgcgacc gctcgagaga
    34561 agaggacggc agcttcgtca taacgtttgg cctcgtaggc caagattcct tcggcaac
//
LOCUS       DAORQN010000006        30075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_98_length_30075_cov_113.976982, whole genome shotgun sequence.
ACCESSION   DAORQN010000006 DAORQN010000000
VERSION     DAORQN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 30075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_30075_cov_113.976982"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1085
                     /locus_tag="PK614_01075"
     CDS             78..1085
                     /locus_tag="PK614_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB80835.1"
                     /translation="MDSSFTLTIDWLAFTVLASNPQETMKVLGGDWSKTKGGFRGYPL
                     SWMRVDGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRTLLKWVHAQQGHVTR
                     IDCALDDRAGTVPVATIREAVSAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELKSKGQENWQEYGTRWELELKKDRAEQCARALASLDEADWKELVV
                     GLLRSYVDFRDITKDTEEEERYRAPVLEWYALLTEGFQKGRLAQEKQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLLNEIVRGIARWKDRHRNLLKEPPNRFHRSAGGHAGSP
                     C"
     gene            1128..1436
                     /locus_tag="PK614_01080"
     CDS             1128..1436
                     /locus_tag="PK614_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB80836.1"
                     /translation="MLTVKELSAWLNIKESTLYLWVSKNKIPCRRIHGLVRFEPEAIQ
                     AWLKSFETSPGKPFSLQTHDDTSDLDQLIEAAKSEVYTRHGETITPSPRTKEEQHGAR
                     "
     gene            1423..2496
                     /locus_tag="PK614_01085"
     CDS             1423..2496
                     /locus_tag="PK614_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB80837.1"
                     /translation="MGLVKRNNTWWMSFTFQGQQVRRSTETSDKKLAEAILSKIRVQI
                     VEGKYFEKPKEDPYTFSQLMDRYLKEHASRRAHYRRYVNMATNVKTFFGNPKLSQITP
                     KTIVAFKAKRYADGVMPATINRELAMLKKAFNLACREWEWAKDNPVCRVSMEKEQNSR
                     DRWLTEQEEARLLMAAAAWLREVVTFAVHTGMRRGEILALTWAGVDFTRRTVTVFKSK
                     NGERRTIPVNQTVLELLSHKYEQGRGLRGIETRVVFCSEAGTELDGSNLRRGFTAALK
                     AAQIEDLHFHDLRHTFATRLVQAGVDLYKVQRLLGHKSPSMTQRYAHHYPESLRDGVE
                     ILDRGKTFSTNLAQRPFAPTVAM"
     gene            complement(2509..2584)
                     /locus_tag="PK614_01090"
     tRNA            complement(2509..2584)
                     /locus_tag="PK614_01090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2549..2551),aa:Thr,seq:cgt)
     gene            complement(2675..3826)
                     /locus_tag="PK614_01095"
     CDS             complement(2675..3826)
                     /locus_tag="PK614_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB80838.1"
                     /translation="MLTGRTRWPRAPRILFLACLWLASVSFSIIVLEWVTGYLYERTP
                     FSNGKRIIDLYLGQQPTVRSDVKGESASLASHPYLLYTHLPNLTTDGYQQTNSLGYRN
                     REFTVAKPPGTIRILVLGGSTTFMWPFVKNPADTWVARLEAKLQTISKKTVQVVNAGL
                     NYGTSAEALAGYVFRHRFLQPDIVIYHGGGNDVLPLFFGNYTPEYTHFRRYGAGEIPR
                     PGEKFLLRHSSTAKYVYSRWLEPIGAVVVTEPFWEVDPKTALERVRTQAPEGFRRNLD
                     MLVSSSQQAGSDVVLFGFLQARRQNLSKNAGAFKGFEEALILGLEKNYDVMREIATRY
                     RVPFVIPPQDRFEDEWFQDNCHLSPAGEEVKAQIMFEQLRDHPIFLSAL"
     gene            complement(3860..5803)
                     /locus_tag="PK614_01100"
     CDS             complement(3860..5803)
                     /locus_tag="PK614_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80839.1"
                     /translation="MRRLSRCPLAFLLFVVWVSSGCQFFGQRSSLATHYNLPLTVQLR
                     MDHSIAAASLDYRDACGQALTLPIRDGLETQLKKRMGQVFERVHLEPGPSTGAVDGVV
                     DTALGVRQVDLFIPRKANKSYPATVMLGMEFSYTDHHGTVLVSKKLQSAATGEVETRA
                     DTCNISGLDKVAEEAAAKLVEGMAQQLGTATKIREQAQFRKSGDKGTMQSAPVPPATP
                     AAAPLAATPVVPVAPLQSVAGSTPQAAAADSAIRSGMTQLSFRTIIRDDNQNHVLEQR
                     ESFSVEFEVKNEGAVVAEGVEVELSGHAAIVGGLKSPVSLGTLQPGEVRRVAVDGKVG
                     AVADAEQAELMCALRASSNVLLPSAKKFFVAIRPDAGAAVEVLSVDVDQLPSRNGKSA
                     QPNSVGIAIGIGAFRDPAVPTVKFAAHDADVMSGYFKTVLGVPPHKVKVLSDAKALKD
                     DLIEVFEQWLPKQGGPQATAYIYVSGRAVVDQETGVVSLLPYDGTRAGASRMFSLARL
                     QRALTKASVKQAVLMLDLSLEPSSGSDPGRVVPPRWVAQDSSGEPDRVMLMIGNSGLQ
                     EAQAYQPGQHGLFTYFLLKGLRGAADLDKSGTVLSGELCEYVHDQVDAVTRGATGSGQ
                     PTLCLPMAGRQPVLTGIPLSKIR"
     gene            5988..6833
                     /gene="ubiA"
                     /locus_tag="PK614_01105"
     CDS             5988..6833
                     /gene="ubiA"
                     /locus_tag="PK614_01105"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRB80840.1"
                     /translation="MADLIRLRNQSGTWLLLLPSLWSLVLANQGRPPMWLLGVFTLGA
                     FVMRSLGVVFNDLADRNFDKHVARTRTRPLAAGRLAPRQALLVALGLAVIAAALVITL
                     NPFTMLLSPIALFLAAIYPFSKRWIQIPQGVLGIAFGWGAVMAWAASRGSIEAPAWWL
                     FAATTCWAIAYDTIYALQDCEDDRRIGVKSSALLFGEAVPVAVGVFLVGMVLCLVVAG
                     QLSGLGMGYYLILALPTGVFLWQVRRLQAPVTPHIAFAMFKQHVYSGVAILVGIWIGT
                     FNQTP"
     gene            complement(6838..7764)
                     /locus_tag="PK614_01110"
     CDS             complement(6838..7764)
                     /locus_tag="PK614_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB80841.1"
                     /translation="MFQNEQSAKGKKLYAHYCMHCHGENGRQNEGFNWSSMTDPKPKD
                     LSNKDEMGTFKDEEIFNTISRDMKDTGPGGEKIGDDEFAVPTMPTFKYTLSEEEIWGI
                     VGYVRSLHGKKLEFNVEGRKKDLQAAQQAADQKFKEAERVAQEAEKKASDEADKKGVE
                     VDDNAYAKEMQAMGQAKKELDQATAAVANFTTRPGKGVNIARPDLNMKPDAAAKLVEV
                     GKQIYVTKYGCNGCHKIGDEGGKVGPALDRAGYRLNPTWVYRWLRNPQAMKPDTRMPS
                     LGLNDADAKAVALYLKTLRAPKPDQPLGKVGE"
     gene            complement(7854..8681)
                     /locus_tag="PK614_01115"
     CDS             complement(7854..8681)
                     /locus_tag="PK614_01115"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRB80842.1"
                     /translation="MARVRSVGKRWWVGAVGLLGLCVAAEVQPAYSQQSVSVRAALVK
                     GSLPADDPTAAAWATAAVSEFPMSPQVHWPVRITDVTAKSVKVRGLHDGTTVAILLEY
                     SDPSEDPEDAAALEFMVGDTKAHFAHGQPMAQVEGGPVNIWYWKNKDGKGADLGAKGF
                     GTLKPHAHQDVKAKGVYQGGVWKVVFSRPLSTEHVAEDTQFKPGTFASIAFAVWDGKK
                     METGQPKEKGSEKAISSWWYFRADAPPDYSPYMYALLAVALALGFELVLVRRLKKGS"
     gene            complement(8722..9447)
                     /locus_tag="PK614_01120"
     CDS             complement(8722..9447)
                     /locus_tag="PK614_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB80843.1"
                     /translation="MGGVLVRPIILTVCIYLFLKFIVPNLPHSAPLPSSLIFLYLMLT
                     TAGIVIFATLSGASKDAFFGPILRFLTGEGGGALSGARYLVLALFPLLVGWQAYGSTA
                     SSDAPPGENRTIHPAPPGEYTGLSNPVPKTLDNINYGKGLYASRCSPCHGNFDGKGFA
                     AAGFTPPPANFKDPTTIAMLQESYLFWRIKKGGVGLPVEGMPWKTAMPRWELEMSDED
                     IWKVIMGEYDGAGQKPRTWDESE"
     gene            complement(9475..10095)
                     /locus_tag="PK614_01125"
     CDS             complement(9475..10095)
                     /locus_tag="PK614_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB80844.1"
                     /translation="MSEVVKLQLIGLCVIGLGTVILLFIKGQFIRVIGFVAIVLGLFS
                     LVALAVPQMASLPPAEESINIADIKTPTDMATIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTEPQAYLYLDYRHEGVPKEYPARMPYINKNPIGLSKNEI
                     LSVIAFLQQMSGEPISVNPTELDLPRATPAPVKAADAGTLAVAQAR"
     gene            complement(10162..12042)
                     /locus_tag="PK614_01130"
     CDS             complement(10162..12042)
                     /locus_tag="PK614_01130"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB80845.1"
                     /translation="MMNQGQIVTNHGSQIAGRVALLAGCAALLAWVGMPDAALAQEAA
                     PQAPTAYRDIPYIGSRNLVWIIAQLHLLLAGFVLGVPIFAWLCEIVGWKSGDKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIGFYPKLMAYLTDIFFPSFLVYCGLFLVETAT
                     LYLYWYGWDAMAEGSGKKFHIFLGFLLNVFAFFIMIVPNSWATFQSSPVVISEGSDFA
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLSAKSQEERYHYDWMGYVGN
                     FIGVFGLLPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWLGITHRIPGSETQYRKPIMAMLIILLLCLGVWMTPHSLVASLEEAQKMGGTHHPLL
                     GVFGVMSAKMTVSNLMVLVTFMSFIMYWRAGKQDTAGWAKAAKALMGGVLGLAAIAVI
                     VLGVWGYFVPAIIRINYFSTSQVGIVIFVMLTITPLTALLLKSAKTTTEMVWGRMPPR
                     AGYALVLNAVMVILLMTLMGYARSSSRVHWHIYGVMRDSSQYAFSPALGYAAALMALN
                     TFLFCMLVAFIFWVATMGDKAKAAASTKGSVEPGAIPAMAGGAPALDGPEADASEEKR
                     PV"
     gene            complement(12071..13093)
                     /locus_tag="PK614_01135"
     CDS             complement(12071..13093)
                     /locus_tag="PK614_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80846.1"
                     /translation="MTGRETMSSFKLGLSILWPACWTALPIKMAFAMLFMAMGTIHLE
                     TKLGITFLMLLMTPVSVFAFFVISLGVGFHFGEGIGLPLLFLLSIPIDIWSLGLVART
                     VFLERLRLEPPESLGIGLWVRFAVAGAIYLPFLWVVQGGATDLARSITKSILDMDMLK
                     AMPVAERIGLEFTAWGSAAFVVLLALTFVGLSILGKLVEGRVAGAQPAGDTYQDLISR
                     WDMIRVPADQSLMLTAFTAAGAVLSLLFWAVLPVSTPHPHECCKPATVEAAPPFDAQK
                     SLSKGEKAFKDAELKIAAIEQRTAEDEKDKQKGGKDKAGVKDGSPKSDGKPSQSAAGK
                     PAGSGK"
     gene            complement(13112..14227)
                     /locus_tag="PK614_01140"
     CDS             complement(13112..14227)
                     /locus_tag="PK614_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HRB80847.1"
                     /translation="MDTTTQPTTTQPSAIEKVFAFVDERVGLKTLQAKMLNEPVPGGS
                     RWAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHSVDYGWFLLSYHFW
                     GSTAMVVCVFAHMSQVFLWGAYKKPRELIWLVGLALFGIVMGFGFTGYLLPWDQRAYW
                     ATTVGVEIMDKTPLLGDFMARFLKGGATPGQMTLSRFFVIHVMVLPAALMGLAGLHLF
                     LFRKAGPAGPFRGSVEEIKAKTDYFFPRQIWKDIVGMVAVFLCICSLAFWEPIVLLEE
                     ATPDPGDYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGAFMALLAALPFIDRS
                     PERNIFKRPIALIGWIVVMASILIFTVAAIINREFLE"
     gene            complement(14228..15166)
                     /locus_tag="PK614_01145"
     CDS             complement(14228..15166)
                     /locus_tag="PK614_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRB80848.1"
                     /translation="MQPKLKSKVRCTDGEVGEVRRVIMDPLSHEISHIVVGAGAADSV
                     ERQVPMGQVQGVAEEVVTLRGTSAEYAALPAFKRDEYVTTHEVEIAHLEERIHVTPGE
                     VLVPFPELERSVKRRTFFMNFTHAIGFLIGLPIAFPVLRYLMKPMYSPFDNQWLKIGN
                     SGKIKQDDVGVQFKFKRKIKEAYMPEQEIDKNVWVLKATPKVLETIYQGKDMEFHDAS
                     GKVIWTNKKDVPYIAYSGKCPHLGCGFKWRTHKTLGQVFLCPCHLSIYDAAGKVLDGP
                     APRALDPLPIKVTAAGDITIIDMEFKAGTKAQVRIV"
     gene            complement(15172..15978)
                     /locus_tag="PK614_01150"
     CDS             complement(15172..15978)
                     /locus_tag="PK614_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB80849.1"
                     /translation="MGNVIKKSLVGIVIGGILFGATNALGFPFVFQVLFFGYAMLGAV
                     VFIILDLPPLKPISGVKAAGALVVFYAILSVAYIAGGAVWPQFDPEDEKGKIDKILKP
                     KREHYEAGKVEVLLQRAKALDEKAKELTARLQALGAAQAPADQKAGGEAAPTATTAAA
                     GGDIAKLGEEQWQLQECYNCHKLNGEGGKKRGPELDNIGNLLSAEEIAIKILDPKSYK
                     AEGFEKEYEKGKMPDKYKDLMEPKDVQALAGWLASYKNASVNTPKPIKMK"
     gene            complement(16018..17721)
                     /locus_tag="PK614_01155"
     CDS             complement(16018..17721)
                     /locus_tag="PK614_01155"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB80850.1"
                     /translation="MKIWQRCLLVMFALVAILGGVAYAQAPSAPPVEFPYTGNRTAVW
                     IVAQLHILFAGFILGAPIFVVISEWLGYRKQDPRYDRLAKEVTKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLIFAVVYPLLFIGETIILYMYFYTWDAWKGEKKARH
                     IALGVLLNLIGSITLFVIDGPTSFMNTPVRAEGVSPAEFLATASLWDKVFNYSWMPLN
                     LHRLVGNVTFGGFIAGLIAAYMFMGSKKEEERAYYDWMGFVGNMIGVGALLFLPFMGY
                     LLSYELCDYDASICPYMMADQLSMFFEMQGAMVGLIFLASNYYIWLSMKRIEGVERVR
                     MTVIAPIVMVLLPIVMTKVLTDYPVPDATSLAFLVPLLLAPVILGRFIPLTVSSSTVI
                     KVGFLMVVVGNAIWMTPHGFVPTGAKLVAELELPSDWNFLALMPAKNSAAFTLIFVTV
                     VNYVIYNRAVSQGTIVWGKIDFASQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHAYNL
                     LPDFTAESFTPTLSYSAWWITGITVTFFAVVSFAIIVTLRPSDSKGHAHEGSPVPAGA
                     K"
     gene            complement(17787..18290)
                     /locus_tag="PK614_01160"
     CDS             complement(17787..18290)
                     /locus_tag="PK614_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB80851.1"
                     /translation="MKQAGMKRLALMVGILGFGFGVAACGGGEGGGEGPVVPPPPAPA
                     EYADKHMPAGWWTDPKIIEEGKELYIGGKNPDVNCASCHGKDGKPVKAGARDFRNGDR
                     MKLYSDSVWFWRISEGVPNTKMKPWKSKLSEEDRWKILAFERSFGLAGKGWDANKKDW
                     VPADQVK"
     gene            complement(18502..19041)
                     /locus_tag="PK614_01165"
     CDS             complement(18502..19041)
                     /locus_tag="PK614_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB80852.1"
                     /translation="MRWRRAISRKTAWRVVVVWGLISTLFVPSVLFASHAADHRFTVE
                     GFVCGKDGRPGANVEVLVKDTRITVGQTVTTDGDGYYKVTLHLHNDNVGDPLLVEVGG
                     EQKNYKIQFDPNDLETERKLRVDFGSGCERDLGPPQWLLYGLGIAAVAIVGWIGLKIS
                     RKRMREEQRRKKGQGKRQK"
     gene            complement(19325..19714)
                     /locus_tag="PK614_01170"
     CDS             complement(19325..19714)
                     /locus_tag="PK614_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80853.1"
                     /translation="MTDAKYHAPETGSLEGRVFRPASLTELAEAIELAFDYRGDITVE
                     LKSGGHVTGYLFNRTATGEQPTIEIFPATSSGTLTIPFSEMVAIAFTGEDTATGKSWE
                     AWSAKKESERRKDIDRAAAEAKSRGHL"
     gene            complement(19816..20832)
                     /gene="hpnH"
                     /locus_tag="PK614_01175"
     CDS             complement(19816..20832)
                     /gene="hpnH"
                     /locus_tag="PK614_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HRB80854.1"
                     /translation="MAVPVSQMYTVTKYVLTQKLRGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPAQCWAAADECAAPIISIPGGEPLIHPEMPEIVRGLVERQKY
                     VYLCTNAILMERKLDEYPPSKFLTFSVHMDGLRDEHDLAVCRDGVYDVAVKAIKAALK
                     RGHRVTTNTTLFDDANPERVRKFFDEMMGLGVEGMMISPGYSYQKAPDQQHFLKRSRT
                     TELFSKILGNRKRGWQFNQSPLFLEFLMGKREYQCTPWGNPTYNVFGWQRPCYLLQEG
                     YVSSFRELMEQTDWDSYGTGRNEKCADCMVHCGYEASAVEDTFGSFSGFAKTVKITLL
                     PNAR"
     gene            complement(20936..22168)
                     /locus_tag="PK614_01180"
     CDS             complement(20936..22168)
                     /locus_tag="PK614_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRB80855.1"
                     /translation="MDWSSDSSPALLDPPRHPAERPTVDQSTNLFDYWDVLVERRKVV
                     AGLCCMGMIVSLVVSFLLPNIYESTSSVLPQLESKEGGALAALLASPAAGGMAQNLGL
                     GLPGLPTTPTDVFVSILKSRLMADDVIHKFNLMDRYREKTMVETRKELEDHLRITVTK
                     EKVIKVAVEDEDPQVAADIANFYVSNLDRLNRTVTVNKAGQNRAFLERRLNETMESMA
                     KAEEALRDFQAKNKTVAVEAQAKVMIEAAAMIQGQITAQEVQLQVMGGYLSPDNPDLA
                     RIRSNVGELKKQLATMGSGKDEKGMLSSERLHPAMVAVPDLALQFGRLFRQVKVQETL
                     FTLLTSQHEQAKIAEARDTPTVQVLDQAVPADKRTRPRILLNVAVAGVLALVIGIFLA
                     FFLDYRVRVRQVSALTRN"
     gene            complement(22324..24933)
                     /locus_tag="PK614_01185"
     CDS             complement(22324..24933)
                     /locus_tag="PK614_01185"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRB80856.1"
                     /translation="MHSSTDESVRSVSHGTVPSRWICVVIWLLVGSIVLPPSLLAQTL
                     TNPVLPPSSAPSAGGPSLGNQMSPFLPFGNYGLPTPPGQAIVTNPTATQPIVPQHAPC
                     PIPTAPDLSPENTVPNLNDYWPMAANSLLPTSVEQRMRQEEEERDRQLERLQAEKEKR
                     GLELQSQVEREKKGVSQLQGLQSAVQGAMGGMQAQNQQNQQRQQVERKPFTAELLRHQ
                     DFSVEEAFAQFSVLQGVKSRLKQFGYDFFDAHAGGFTSLQDVPVGPDYVIGPQDSLAV
                     HIWNVPDQNFNRSYIAPVERDGMVVIPQVGAISVGGQTFSQAERTIHARLSMLLKRFE
                     LHVSMARIRTMKVYVVGEVARPGAYELSALATASNAIYAACGPSRSGSLRQIRIMREG
                     KAVGQLDLYDFLLQGDRRQDNRLQAGDVVLVPPLGTVVAISGSVKRPAIYEMKPGSRL
                     TELLTLAGGLTPLSDRQRCHLFRQDPALQERNMIDVDLVRAFASQGPEKSRLGVEGGD
                     PILLDGDYVRIATLPTQVVNVVSLVGAVKSPGPYEYRAGMHVKDILSPEQLTVDAYGD
                     KAEIIRTDPTTYLTKVIPFSPKAVFEGRESDNHVLNRLDQVVIASQMRPPALVLVEGE
                     VRRAGYFTIEIGERLSSVLKRSGGFTQNAFPNGIVLVRESVKLKQQAELDRFIAAERQ
                     RLTAQSAGIAAGTAGLSVVAAAGGTMAEQQVLALRLQQLEATASRIELGRVIVSLDSI
                     ERLEGTEDDIILESRDRITIPTPPQTVGIIGSVKNPSTVVYRPGLELNDYLRQAGGIT
                     EDANKREMYVMRANGTTDSSYLSAKEMRPGDTIVVPQKIEARPPQLALWQTVASIIGS
                     LALTAAGIAVVGR"
     gene            complement(25024..26658)
                     /locus_tag="PK614_01190"
     CDS             complement(25024..26658)
                     /locus_tag="PK614_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HRB80857.1"
                     /translation="MKIRRLSFALSLGLALWLLLNGIALASSNVPLHHWSYDAIERLI
                     GLGVIDRALIGAKPFSRMQAAQYVARAVERVRADQVELDGREAIAEPLLERLLAEFRS
                     ELIRLGTVRARPDDKTGALRFGARVTSEFDASSIGGGQTVRFRENRGGEYSVNGVQNQ
                     TDVRAWAELSDWAAVMVQPKFISNRHLLGLGATNNSENFYLREFSLKLSYANVALEVG
                     RGTQWWGPGYHGSLLLTDHAFPMEMIKLGTERPFQLPGFLSGLGDWKVNAFLGRLEEN
                     RDFPRAKLFGVRLSYLPASWLEFGLTRLTQFGGRGRDQSFPGVVFDAYISEPNQTGNR
                     DVNEQAMADFRLRIPSIPYLIPFPAGLQLYGEAGTEDKWSQLPVPSRTAFLGGIYIPQ
                     VFQGDTLDLRIEYADTDYGRRRHPELRQVWYNNSPYISGMRYRGFPLGHHMGTDGTDL
                     FVRTTRYLTDTLQLGANFNLQERDRGQPVHEKKREAAVDLTWWYSKDVQFMGGYTFQR
                     LTNPGQVSAITPFAETFSAGVTAKNHLLWTAVAVQF"
     gene            complement(26744..28735)
                     /locus_tag="PK614_01195"
     CDS             complement(26744..28735)
                     /locus_tag="PK614_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRB80858.1"
                     /translation="MAQLGYLVARPFLPSFSAVQRRLLLFVGHLGLAALSNWVAFLLR
                     FDGNIPVQEWDLLVAMLPLLLIIRAFTFYPFRLYDGIWRYTGLWDLRNLALSIAVSSL
                     AFAGVVRFGLGTRAYPSSIFVIDALLLWCLLAGLRVLPRLIREAGWFRTDRKRVLIVG
                     AGDAGAMIVREMRNHPSYGYRPIGFVDDNPVKVGHRIHGVRVLGDRDCLSHIIARQAP
                     DVVLVAMPSAKPAAIRAVVKVLEPFHLPIQTLPNLRDLLQCRVEVSQIRNLSIEDLLD
                     RVPVDLDPEPLRALVQGERVLVTGAGGSIGAELCRQVAGLAPASLVLLDRYENGLFAV
                     VNELVAAGKTFVAPVIGDVTEVGQMNRLFAEYRPTLVFHAAAHKHVPLMEGNPCEAVL
                     NNVGGTRVVAEAAHRHEVDRFILISTDKAVNPVSVMGASKGVAELLVQRLARTSQTVF
                     ATVRFGNVLGSNGSVVPLFLEQIKAGGPVTITDPNMRRYFMLMTEAVHLVLQAARLAR
                     GGELFVLEMGEQISVVEMARNLIRLSGLVPDKDIALTYLGCRPGEKLTEELVAGHETV
                     ESTTVQKVLCIRPDPARSFAQLSGLVARLEESAAEGHVSKVLALLCAILPTYCPAGTS
                     LIQERGGETDGLLSEDPAQSPAGIETGAGLAPTAPLRRK"
     gene            complement(28885..29613)
                     /locus_tag="PK614_01200"
     CDS             complement(28885..29613)
                     /locus_tag="PK614_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB80859.1"
                     /translation="MTPEALSMVYTRYRFAVDFCRGRRVLEVACGPGVGLGYLGRHAD
                     GIVGGDLTDRLLLQAKRNLQSTRPLVRLQAEALPLHTASRDVIICYEALYFFEDAGKF
                     LKECRRVLAPPGVLLLCTVNPEWPEFNPNARSCRYYSARELAALLREAGFQPDIFGAF
                     SIAPASIPAACLSWIKRVAVRLRLIPSSMAGKRVLKRLFLGKLVPFPAAVDEDMVVYA
                     PPVSISPEQPATSYKILFAVGRPV"
     gene            complement(29658..>30075)
                     /locus_tag="PK614_01205"
     CDS             complement(29658..>30075)
                     /locus_tag="PK614_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB80860.1"
                     /translation="LDNVQAAVLDVKLRHLPDWIAHRRRIAAAYHRGLSGITQLRIPQ
                     FDETRRKDIFQNYVIRTMVRDQLRAYLKEQGIETLVHWQKPMWEHEGLGLAVPDVPET
                     RSICREVVSLPMSAETREVQVELIVAALRAFFSTQP"
BASE COUNT         6789 a         9225 c         8062 g         5999 t
ORIGIN      
        1 gcggagcgga atggcccccg tcttggtaac acgggggcgc tacccacagc gctccgtaca
       61 ggagttcgat agattccatg gattccagct tcaccctcac cattgattgg ctggcgttta
      121 ccgtcctggc gagcaatccc caagagacca tgaaggtcct cggtggcgac tggagcaaga
      181 ctaaaggcgg cttccgaggc tatcccttgt cttggatgcg ggtagacgga ctgcgcgggg
      241 tcggtaaatt gggcaccaat gcgcctcgcc gtccgaatga aatccatgtg gatctttcgg
      301 gcggcctcgc gtccgccctg acattggatc aaatccgcac cctcctcaag tgggtccatg
      361 cccaacaggg gcacgtcact cggattgatt gcgcgctgga tgaccgagcg ggaacggtgc
      421 cggtggcaac aattcgagaa gccgtctcgg caggccaatg tgtgaccagg gccgctcagg
      481 tgcggcatat cgtgtccaac ctgacgcatg ggacgggagc gacgacgggc gagacgatgt
      541 acttcggcag tccgcagagt cagaccttgt tgcgcattta cgacaaacga ctcgaactca
      601 agagcaaggg acaagagaac tggcaggagt acgggactcg gtgggaattg gagttgaaga
      661 aggatcgagc cgaacaatgt gcaagagcct tagcctcatt ggatgaagcc gattggaagg
      721 agttggtggt cggcttactc cggtcctacg tcgatttccg ggacattacc aaagacaccg
      781 aagaggaaga acgatatcgg gctccagtgt tggagtggta cgccctcctg acggagggat
      841 ttcagaaggg gcgattggcc caggagaagc aggtgcagac cctgcagaac gtgaaacgat
      901 gggtgagtga caccctgacg ccgatgttgg cggtgatttg cgccacccct ggaggggaag
      961 aatggctact gaacgaaatt gtcaggggca ttgctcggtg gaaagaccga caccgcaatt
     1021 tgctcaagga accacccaat cggtttcacc ggtctgccgg cggtcacgcg ggcagcccat
     1081 gttaggggga ctgggggtgt cggcagactc ccccggtgta tctgcagatg cttaccgtca
     1141 aagaactctc agcttggctc aacattaaag aatccacact ctatctctgg gtttccaaga
     1201 acaaaatccc atgccgtcgc atccacggcc tcgttcgttt tgaacctgag gccatccagg
     1261 cgtggctgaa aagctttgaa accagtcccg gcaagccgtt ctcactgcag actcacgacg
     1321 acaccagtga cctagaccag ctcattgaag cggcgaaatc cgaggtctat actcgccacg
     1381 gggaaaccat cacaccgagc ccacgcacga aggaggagca gcatggggct cgttaaacgg
     1441 aacaatacct ggtggatgtc atttacgttt caagggcagc aagtacggcg atcgacggaa
     1501 acctcggaca aaaagttggc cgaagccatt ttgagcaaga tccgagtgca gattgtcgaa
     1561 gggaaatact tcgaaaagcc gaaagaagac ccctacacct tttcccagtt aatggacagg
     1621 tatctgaaag agcatgcgag tcggcgcgcc cactatcgac gctatgtgaa catggcgacg
     1681 aatgtgaaga cgttcttcgg gaatccaaaa ctttcccaga ttacgccgaa aaccatcgtc
     1741 gccttcaagg cgaagcgcta tgcggatggc gtcatgccgg ccaccattaa ccgagaattg
     1801 gccatgctga aaaaggcttt caacctggcc tgtcgcgaat gggaatgggc gaaggacaat
     1861 ccggtctgtc gggtgtcgat ggagaaggag caaaactcac gggaccgctg gcttaccgaa
     1921 caggaggaag cacggctact catggctgca gccgcctggc ttcgggaagt ggtgaccttc
     1981 gccgtccata ccggcatgcg acggggagaa attctggctc tcacatgggc aggggtggac
     2041 ttcacccgac gaaccgtgac ggtgtttaaa tccaagaacg gggaacgccg gactattccg
     2101 gtcaaccaaa ccgtcctgga actgctctct cacaaatacg agcagggccg tgggctacgg
     2161 ggaattgaaa cccgggttgt gttctgcagt gaagccggga ctgagttgga tggcagcaac
     2221 ctcagacgcg gatttaccgc agccctgaag gcggcacaga ttgaggacct tcatttccac
     2281 gatttgcgcc acacctttgc tacgcggctg gtccaagccg gagtggacct ctacaaagtc
     2341 cagcggctgc tcgggcacaa atctccgagc atgacccaac ggtatgcgca ccactatccg
     2401 gaaagtctgc gggatggggt ggagattttg gaccggggaa agacgtttag cacaaattta
     2461 gcacaacgac catttgcgcc aacggtggca atgtaagtgc ttgataaatg gagccggcga
     2521 gtggaatcga accaccgacc tgcggtttac gaaaccgctg ctctgccaac tgagctacgc
     2581 cggcttgttg acctgaacat catcttcgga tcggtcaagg agcgcaatct tactcagggg
     2641 atggaagcac tgtcaatgat tgaaacgcct cccgttacag agcagataga aaaatgggat
     2701 gatcccgtaa ttgttcgaac atgatctgcg ccttgacctc ctcgcccgct ggagagaggt
     2761 gacaattgtc ctgaaaccat tcatcctcaa accgatcttg gggcggaata acaaagggca
     2821 cccgataccg ggtcgcgatt tcccgcatga cgtcatagtt cttttccagc cccagaatca
     2881 gagcctcttc gaaccctttg aaggctcctg cattcttgct gagattctgc cttctcgctt
     2941 gcaagaaacc gaacaacacc acgtctgagc cagcctgttg actcgaagac acgagcatgt
     3001 cgagattccg tcggaaccct tcgggcgcct gcgttcgtac ccgttcaagc gcagttttcg
     3061 gatccacttc ccaaaacggc tccgtcacga cgaccgcacc gatcggctcc agccacctgg
     3121 agtagacgta ttttgctgtg ctgctgtgcc gcagcagaaa tttctctcct gggcgtggaa
     3181 tctcacccgc cccataccgt ctgaagtggg tatattcggg agtgtaattc ccgaaaaaaa
     3241 gcggcagtac gtcgttgccg ccgccatgat agatcacgat atcgggctgg agaaaacgat
     3301 gtcgaaacac atagccggcg agcgcctcgg cgctggttcc gtagttcagt cccgcattga
     3361 ctacctgaac cgtcttcttc gaaatcgtct ggagtttagc ctcgaggcgg gccacccacg
     3421 tatccgccgg gttcttcacg aacggccaca tgaaggtcgt cgatcccccc agcaccagaa
     3481 ttcgaatggt gcccggtggt tttgcgacag tgaattctct attccgatag ccaagcgaat
     3541 tggtctgttg gtacccgtcc gtcgtcaaat tgggcagatg cgtgtagagc aagtagggat
     3601 gggacgcgag tgacgcgctc tctcccttta catcagagcg aacggtcggc tgttgcccca
     3661 ggtacagatc gatgatccgc tttccattcg agaagggcgt gcgctcatag aggtaccctg
     3721 tgacccattc cagaacgata atcgaaaaac tgacgctggc tagccagaga catgccagga
     3781 agaggattcg aggggcccgt ggccatcgtg ttcgtccggt cagcatcacc accgccttcc
     3841 ggaaggtaga gtgccaccct cagcggatct tactcagagg aatcccggtt aacaccggtt
     3901 gccgaccagc catcggcaga cagagtgtgg gctggccact ccccgtcgct cctcgcgtaa
     3961 cggcatcgac ctgatcatgc acgtattcgc agagttcccc ggacaacaca gtcccgctct
     4021 tgtcgagatc ggccgctccg cgcaacccct tcagcagaaa atatgtgaac aacccatgtt
     4081 gtcccggctg ataggcttgc gcctcctgta ggccggagtt tccgatcatc agcatgaccc
     4141 ggtctggctc cccgcttgaa tcctgcgcga cccatcgcgg agggaccacc cggccagggt
     4201 cggaaccgga cgacggctcc agggagagat ccagcatcaa cacggcttgc ttcacggagg
     4261 ctttggtcag cgcccgctga agccgggcga gggaaaacat ccgggatgca ccggctcgcg
     4321 tcccatcgta aggcaacaac gagacgaccc cggtctcttg atccaccaca gcccggccgg
     4381 acacataaat atacgccgtt gcctgcggac cgccctgctt cggcaaccac tgctcaaaca
     4441 cttcgatcaa gtcatccttg agcgctttcg cgtcggacag caccttcacc ttgtgcgggg
     4501 gaacacctag aacggtcttg aagtacccac tcatgacgtc agcatcgtgc gcggcaaact
     4561 tcaccgtcgg cacggccgga tcccggaacg cgccgatacc gatcgcgatc ccgacactgt
     4621 tcggctgcgc cgattttcca ttcctgctcg ggagttggtc cacatctacc gacaatacct
     4681 ctacagcggc accggcatca ggacgtatcg cgacaaaaaa cttctttgct gacgggagca
     4741 ggacgttgga tgacgctcgc agggcacaca tcaactcggc ctgctcggcg tctgcgaccg
     4801 cccccacctt gccgtcaacg gccacgcggc gaacttcacc cggctgcagc gtccccaacg
     4861 agaccggcga cttgaggcct ccgacgatcg cggcatgacc gctcaattca acctcgactc
     4921 cctccgcaac tacagccccc tcgttcttga cttcaaactc cacactgaac gactcacgtt
     4981 gttccagaac atgattctgg ttatcgtctc ggatgatggt ccggaacgag agttgagtca
     5041 tcccggatcg gatagccgaa tccgcggctg cggcctgcgg cgtggagccg gcaacagatt
     5101 gcaatggagc taccggaacc accggtgtgg ccgccaatgg agccgctgcc ggtgtcgcag
     5161 gcggcacggg agcagactgc atggttccct tgtcgccgct ctttctgaac tgtgcctgct
     5221 cgcgaatttt ggtggcagtg ccaagctgtt gggccatacc ttccacaagc ttggcggccg
     5281 cctcttccgc cactttgtcg aggccgctga tattgcaggt atcggcccgc gtctcgactt
     5341 ctccggtcgc agcgctctgc aacttcttgc tcacgaggac cgtgccgtgg tgatcggtat
     5401 acgaaaactc catccccagc atcaccgtcg caggatagga tttattggcc ttacgaggaa
     5461 tgaacagatc cacctggcgg actcctaatg cggtatccac cacgccatcc acggcaccag
     5521 tcgatgggcc tggttcaagg tgcactcgtt cgaatacctg ccccatgcgc tttttcaatt
     5581 gcgtctctaa cccgtcacga atgggcaggg tgagggcctg gccacaggca tcgcgatagt
     5641 ctagcgacgc cgcagcgata ctgtgatcca tccgaagctg gacggtgagt gggagattgt
     5701 aatgagtggc caggctgcta cgctgaccga agaattggca tccgctcgac acccatacca
     5761 cgaacagcag aaaggcgaga ggacacctcg acagccgccg cacacttgtt cctattcctg
     5821 ggcacataga ttgctgcaca atagtttcgt tataccgaat ctcacaaaga cataacaacc
     5881 gacgtgatgc cgcgttgaca gtcctcctgt gctcggctat ggtccagcgc gtgaatgctg
     5941 cgagtccttc aactcaccgc tcaacacccg catggtcctg gagcgcggtg gccgacctga
     6001 tccgactgcg gaatcaatcc ggcacctggc tcttactgtt gcccagcctg tggtcgttag
     6061 tcctggcaaa tcagggccgt ccaccgatgt ggctgttggg cgtctttacg ttgggcgcct
     6121 tcgtgatgcg gagtctcggc gtggtcttta acgacctggc cgaccgcaat ttcgacaaac
     6181 acgtcgcacg cacacgcact cgaccgctcg ccgcaggccg ccttgcgccc agacaagcgc
     6241 tgctcgtcgc acttggcctg gcagtgatcg cggccgctct ggtgatcaca ctcaatccct
     6301 tcacgatgct actgagtcct atcgccctct ttctggcagc gatctacccc ttcagtaagc
     6361 gatggattca gattccgcaa ggggtgctgg gcattgcctt cggttgggga gccgtgatgg
     6421 catgggcggc ctcgcgcggc tcgatcgaag cgccggcgtg gtggctcttt gccgcgacga
     6481 cctgctgggc catcgcgtac gacacaattt atgcgctgca ggactgcgag gatgatcgcc
     6541 gcatcggagt gaagtcgtcg gcacttctct tcggtgaagc tgtcccggtc gccgtcggag
     6601 ttttccttgt ggggatggta ctgtgcctgg tagtggccgg acagttgtcc ggcctcggga
     6661 tgggatatta tctaatattg gccctgccga ccggagtctt cctctggcag gtacgacgat
     6721 tacaagcgcc cgtcactccg cacatcgcct tcgccatgtt caaacagcat gtctattccg
     6781 gcgtggcgat cctcgtcggt atctggatcg gcacctttaa tcagactccc taaccggcta
     6841 ttcgcccacc ttcccgagtg gctggtctgg cttcggagcg cggagggttt tcagatagag
     6901 agccacagcc tttgcatccg catcgttcaa tcccaagctg ggcatacgcg tatcaggctt
     6961 catggcttgc gggttgcgaa gccatcggta gacccaggtg ggattcagac ggtatccggc
     7021 acgatccagc gcaggcccaa cctttccacc ctcatcgccg atcttgtgac atccattgca
     7081 cccatacttg gtcacataaa tctgcttgcc gacttccacc agtttcgcgg cggcatccgg
     7141 cttcatattc agatcaggac gcgcaatgtt tacgcccttg ccgggcctgg tcgtaaaatt
     7201 cgccactgcg gccgtggcct ggtcgagttc ctttttggcc tgccccattg cctgcatttc
     7261 tttggcatag gcgttatcgt cgacttcaac acccttcttg tcggcttcgt cgctggcttt
     7321 cttctccgct tcctgagcca cccgttccgc ttccttaaat ttctgatcgg ctgcctgctg
     7381 ggcggcttga aggtctttct ttcgaccttc aacattgaac tctaatttct ttccgtgcaa
     7441 cgagcgcaca tatccgacga tgccccagat ttcttcctct gacagagtgt acttgaaggt
     7501 cggcatggtc gggacggcaa attcatcatc accgatcttt tccccgcctg gtcctgtgtc
     7561 cttcatatcg cgggaaatgg tgttaaagat ttcctcgtcc ttaaaggtgc ccatttcgtc
     7621 tttattggaa aggtctttgg gcttcgggtc cgtcatggac gaccaattga acccttcgtt
     7681 ctgtctgcca ttttcaccgt ggcaatgcat gcaatagtga gcatagagct tcttgccctt
     7741 cgcggattgt tcgttctgaa acatcgaaca acccccggct ccaatgaggc ccgagagtcc
     7801 caccaccact gcgaccgctc caaatacgcg tattcttcgc cacgccctca tgctcacgac
     7861 cctttcttta aacgtctcac gaggaccaac tcaaagccca gcgctaaggc caccgcgagt
     7921 aatgcataca tgtacggcga atagtccggc ggcgcatcgg cccggaaata ccaccaggac
     7981 gaaatggcct tttcagatcc cttttccttc ggctggccgg tctccatctt cttgccgtcc
     8041 caaacggcga aggcgatgct cgcgaacgtg cctggcttga attgggtatc ttcggccaca
     8101 tgctccgtgc ttaacggacg cgagaaaacc actttccaga cgccaccctg gtagacacct
     8161 ttggctttga catcctggtg ggcatgaggc ttcagagttc cgaacccttt tgcacccagg
     8221 tctgcgccct tgccgtcctt gttcttccaa taccagatat tgaccgggcc gccctccact
     8281 tgagccatgg gctggccgtg ggcgaaatgg gccttggtat cgccgaccat gaactccaag
     8341 gctgctgcgt cctccgggtc ttcagacgga tccgaatact ccagaagaat cgccaccgtg
     8401 gtaccgtcat gcagtccgcg aactttgacg ctcttggccg tgacgtcggt aatacgaacc
     8461 ggccaatgca cctgaggaga cattgggaat tccgataccg ccgccgtggc ccaggccgct
     8521 gccgtcggat catctgccgg aagggatcct ttcacgagcg cggcgcgcac cgacacgctc
     8581 tgctggctat aggccggctg cacctcggcg gccacgcaca gccccagaag gccgacggct
     8641 ccaacccacc atctctttcc tacgctcctc actcgtgcca tacgctcccc ttaattgctc
     8701 agcaatctct gatcggagga attactcgga ctcatcccac gttcttggtt tctgtccggc
     8761 cccgtcatat tcgcccataa taactttcca gatgtcctca tcggacattt ccagttccca
     8821 tcgcggcata gccgtcttcc atggcatccc ttcgacagga aggcctacgc ctcccttctt
     8881 gatccgccag aagagatagc tttcctgcag catggcgatc gtcgtcggat ccttaaagtt
     8941 agccggcggc ggcgtgaatc ccgccgcagc gaaacccttc ccatcgaagt tgccgtggca
     9001 tggcgagcaa cgcgaggcgt agagcccctt gccataattg atattgtcca gcgtcttcgg
     9061 aacgggattg gaaagaccgg tatactcacc gggaggagcg ggatgaatcg tgcgattttc
     9121 acccggtggt gcatcactgg aggccgtgct cccgtaggcc tgccatccga cgagcaaggg
     9181 gaataaggcc aacacgagat accgtgctcc actcagggcc ccgccacctt ccccagtgag
     9241 aaagcgcagg atcggtccaa aaaatgcgtc ttttgatgcc ccgctgagcg tagcaaaaat
     9301 gacgatcccg gccgtcgtca gcatcaggta caggaagatc aagcttgagg gcagcggcgc
     9361 ggaatgcggc agattcggga caataaactt taagaacaga taaatacaga ccgtcagaat
     9421 aatcggtcga acgagtacgc ctcccataac taccctcctc tacttcaccg acgactatcg
     9481 agcctgtgcc accgcgagag ttccggcatc cgccgccttg acgggagccg gggtcgcccg
     9541 cggcaaatcc aattccgtcg gattcacgct gatcggctca ccgctcatct gttgcaggaa
     9601 cgcaatgacc gacagaattt cgttcttgga caggccgatt ggattcttgt tgatatacgg
     9661 catccgggcc ggatactcct tcggaactcc ctcgtgtcgg tagtccagat aaagatacgc
     9721 ttgcggctcg gtcagactct caaaaataaa ctcgcgggac agcttggctc cgatcccctt
     9781 cagatccgga cagcgtgccg attcgctcgg gccgatagaa tggcagagcg cacactggcc
     9841 cttgctgaag aagatcttct gcccaatcgt cgccatgtcc gtcggcgtct tgatatcggc
     9901 gatgttgatg ctttcttcag caggcggcaa tgaggccatc tggggaacgg ccagtgcgac
     9961 caatgaaaac agtcccagca cgatcgccac gaatccaatg acccgaatga attgcccctt
    10021 gatgaacagg aggatcactg ttccgagtcc gatgacacag agaccgatta actgcagttt
    10081 gactacttca ctcataagtc ctcaacaaag gataatgatc acgacccacc cctggtaacc
    10141 aggggaagat catcttcccg atcagaccgg acgtttttcc tcagaagcat cggcctcagg
    10201 cccatccagc gccggcgccc ccccggccat cgcggggatg gctcctggct cgaccgaacc
    10261 tttggtgctg gcagcggcct tggccttgtc tcccatcgtg gcaacccaga aaataaaggc
    10321 caccaacata cagaacaaga acgtgttcag cgccatgagc gcggccgcat aaccaagcgc
    10381 gggagaaaac gcgtattggg aggaatcgcg catcacaccg tagatgtgcc agtgaacgcg
    10441 ggatgaagaa cgcgcataac ccatgagggt catgagcagg atgaccatga ccgcgttcaa
    10501 taccagcgca tagccggcgc gtggtggcat tcttccccac accatttcgg tggtggtctt
    10561 ggcgctcttg agaaggagcg cagtgagcgg agtaatcgtc agcatcacaa agatgacgat
    10621 acccacctgg gacgtagaga agtaattgat acggataata gcgggaacga aataacccca
    10681 gacaccgagc acgatgactg ctatggctgc caacccgagc acaccaccca taagagcctt
    10741 ggcggccttg gcccatccgg cggtgtcttg cttaccggca cgccaataca taatgaagct
    10801 catgaatgtg acgagcacca tgagattgga gacggtcatt ttggccgaca tgacgccgaa
    10861 tacgcccaac aagggatggt gcgtgccgcc catcttttgg gcctcttcca agctggcaac
    10921 gagggagtgc ggggtcatcc acacaccgag gcagagcagg agaatgatca acatcgccat
    10981 aatcggtttg cgatattgcg tttccgaccc cgggattcga tgggtgatac cgagccagaa
    11041 gtagtagttg gagcccagga agagcacacc gatcaacatg gcctgcagaa tgaacaacca
    11101 ggagaggaat ccgcccatga gcgtgatacc catctgctgg ttgtactgat aaatttcccg
    11161 catcagccag tacccggcga atggaagagg caagagtccg aacacgccga taaagttccc
    11221 gacgtatccc atccaatcgt agtgatagcg ctcttcctgg ctcttggccg acagataccg
    11281 gatgccggcg tacgcgccac aaatataacc accgagcacc acgttggcga tcaagcggtg
    11341 aatgttgacc ggccaccagg tcggattcca cgtcgccgcc caggcccgcg cgaaatcgct
    11401 gccctccgag atcaccactg gactggactg gaacgtcgcc cacgagttgg gcacgatcat
    11461 gataaagaac gcgaacacgt tgaggagaaa tcccaggaaa atgtgaaact tcttcccgct
    11521 accctcggcc atggcgtccc acccgtacca gtacaggtac agcgtggccg tttccacaag
    11581 gaagagaccg cagtagacga gaaaggatgg gaaaaagata tccgtgagat aagccatcaa
    11641 cttcggatag aagccgatga gcaggaacaa caggataccg ccgaacagtg ccgtcgtggc
    11701 gtatgaagac gtcaagagct tggtaaactc tttggccagc ttgtcataac gtttatcgcc
    11761 tgatttccag ccgacgattt cgcacaacca ggcaaagatc ggcacgccaa gcacgaagcc
    11821 ggcgaggagc aaatggagct gggcgataat ccagacaagg ttgcgactgc cgatatacgg
    11881 aatatctcga tacgcggtcg gagcctgcgg ggcagcttcc tgagcaaggg ccgcatccgg
    11941 cattcccacc catgccagca gtgccgcaca tccggccaac agggccacac ggcctgcgat
    12001 ttggctcccg tgattggtga cgatctgacc ctggttcatc attgttctca cctcgtcatt
    12061 cgccatgatg tcatttgccg cttcccgccg gtttcccagc tgctgattgc gaaggcttgc
    12121 cgtcgctctt gggagagcca tccttgacac cggctttgtc cttgccgccc ttctgcttgt
    12181 ctttttcgtc ttctgccgtt cgctgttcga tcgcggcaat cttcagctct gcgtccttga
    12241 acgccttctc tcccttagag agggactttt gagcatcaaa gggcggagca gcctccacag
    12301 tggcaggttt acaacattcg tgcggatggg gagtcgacac cggaagcacg gcccaaaata
    12361 ggagactcaa tactgcgccc gcggcggtga aggcggtcaa catcaagctc tgatcggcgg
    12421 gcacacgaat catatcccaa cgcgatataa gatcttgata ggtgtctccc gctggttggg
    12481 caccggcaac acgaccttca accagtttcc caagaatcga caggccgaca aaggtcagcg
    12541 ccagaagcac gacgaatgcc gcactgcccc aggccgtgaa ttccagaccg attcgctcag
    12601 cgaccggcat agccttgagc atatccatat ccaaaatgga tttcgtaatt gaacgggcga
    12661 gatcggtcgc gccgccctgt accacccaca agaagggaag gtagatcgcc ccggcgaccg
    12721 caaaccgaac ccacagccca atgcccagtg attcgggggg ctcgagccgc aagcgttcca
    12781 aaaacacggt acgggcaacc aagcccaagg accagatgtc gattggaatg gagagcagga
    12841 acaataaggg aagcccgatc ccttccccga aatggaaccc gacacctagg gagatcacga
    12901 agaacgcaaa gacgctgacc ggagtcatga gcaacatcag gaatgtaatc ccgagtttcg
    12961 tctccagatg gatcgtcccc atcgccataa acaacatggc aaatgccatc ttgatcggca
    13021 gggcggtcca gcaggccggc cagagaatgc taagccctaa tttaaatgac gacatcgtct
    13081 ctcgaccagt catgtgccca aacccctccg cctattcgag aaattcgcgg ttgatgattg
    13141 ccgccacggt gaaaatcaga atgctcgcca tgaccacaat ccatccgatc aaggcgatcg
    13201 gtcgcttgaa gatattgcgt tccgggctgc gatcgatgaa cggcaatgcc gcgagcaacg
    13261 ccatgaacgc gccgggcaag gcaatggcgc cgaggaactg cccgaactct ccggcaaaca
    13321 ccttcagacg cagcagttgg aacaagaaga ggaagtacca ttccggttcc gggtggtaat
    13381 ctccgggatc cggcgtggcc tcttcgagga gcacaatcgg ctcccagaac gcgaggctac
    13441 agatacacag gaaaaccgcg accatcccga caatgtcttt ccagatttga cgggggaaga
    13501 aatagtcggt cttggcctta atttcttcca cgctaccccg gaagggtccg gcagggccag
    13561 ccttacggaa caggaacaga tgcagtcccg cgagccccat caaggccgct ggaagaacca
    13621 tgacgtgaat gacgaagaag cgactcagcg tcatctggcc tggcgtcgcg ccccccttca
    13681 ggaagcgagc catgaagtca ccgaggagcg gcgtcttgtc catgatctca acgccgaccg
    13741 tcgtggccca gtaggcccgt tgatcccacg gcagtagata gccggtaaag ccgaatccca
    13801 tgacaatgcc gaagagggcc aaaccgacca accagatcag ttcgcgcggt ttcttgtagg
    13861 cgccccaaag aaagacttgg gacatgtggg cgaacacaca gacgaccatc gctgtggatc
    13921 cccaaaagtg atagctcaac aagaaccacc cgtaatccac cgagtggatg atgtattggg
    13981 tgctcgcata ggcatggtcg gccgtcggca cgtaatagaa catcaacaga atgccagtaa
    14041 ccgcctgcat gatgaaaata aagagcagga cggagccaaa tacatatgcc catcgagacc
    14101 cgccgggaac gggctcgttg agcattttgg cttgcagagt cttcagcccg acgcgttcgt
    14161 cgacgaaggc gaagactttt tcaatggctg agggctgagt ggtcgtcggc tgcgtagtcg
    14221 tatccattta gacaatccga acctgggcct tggtgcccgc cttgaattcc atatcgataa
    14281 tggtgatgtc gcccgctgca gtaaccttaa tcgggagcgg atccaacgca cgcggagccg
    14341 gcccatcaag gacctttccg gctgcgtcat agatgctcaa gtgacaggga cacagaaaga
    14401 cctgcccaag cgtcttatgc gtccgccact tgaaaccgca ccccaggtga ggacacttgc
    14461 cggaatacgc gatgtagggc acgtctttct tattggtcca gatgaccttc ccggatgcgt
    14521 cgtgaaactc catgtcttta ccctgataaa tcgtctccag cacctttgga gtcgccttca
    14581 agacccagac gtttttgtcg atttcctgct ccggcatgta ggcttcttta atttttcttt
    14641 tgaacttgaa ctgcacgccg acgtcatcct gcttgatctt tccggagttg ccgatcttga
    14701 gccattgatt gtcgaagggg gaatacatcg gcttcatcag gtatcgcagc acgggaaatg
    14761 cgatcggcaa accgatgaga aatccgatgg cgtgagtgaa attcatgaag aaggtacgcc
    14821 gcttgacgct gcgctcaagc tccgggaagg gaaccagcac ttcacccgga gtgacatgga
    14881 tgcgctcctc caggtgagca atttccacct catgagtggt cacatactcg tcacgcttaa
    14941 acgccggaag tgccgcgtac tctgccgagg tgccacggag cgtcacgacc tcttcggcga
    15001 ctccttgcac ctgccccata ggaacctggc gctcgaccga atccgctgcg ccggcgccga
    15061 ccacgatatg gctaatctcg tgggagagcg ggtccataat gaccctgcgc acctccccga
    15121 cttcaccgtc cgtacatcga actttggatt tcagtttcgg ctgcatgatg ctcacttcat
    15181 cttgatgggc ttcggagtat tgaccgaagc attcttgtaa ctggccaacc aacccgccag
    15241 cgcctgcaca tccttgggct ccatcaggtc cttgtacttg tcgggcatct tccctttttc
    15301 atactccttc tcgaagcctt cggccttata gctcttcggg tcgagaatct ttatcgcgat
    15361 ttcctcagcc gacaggaggt tgccaatgtt gtccaattcc gggccacgct tcttaccgcc
    15421 ttcgccgttc agcttgtgac agttgtagca ctcctgaagt tgccattgct cctcaccgag
    15481 cttggcgata tcaccacccg cagcggctgt cgtcgccgtc ggtgcagcct ctccaccggc
    15541 cttttgatcc gccggagcct gcgcagcacc caacgcctgc aaacgcgccg tcagttcttt
    15601 ggccttttca tcgagcgcct tggcgcgctg caacagaacc tcgactttgc cggcctcgta
    15661 atgttctcgc ttcggcttca agatcttgtc gatcttgcct ttttcgtctt ccggatcaaa
    15721 ctgcggccat acagcgccac ctgcaatata ggcgaccgac agaatggcgt agaacacgac
    15781 gagggctccc gcagctttta ccccgctgat cggcttgagc ggagggaggt cgagaataat
    15841 aaagacgacg gcgccgagca tggcatagcc aaaaaacagc acctggaaca caaacggaaa
    15901 tcccagtgcg ttggtggctc cgaatagaat tccgccgatc acgattccaa ctagcgactt
    15961 cttaatcacg tttcccatga gtaccctcgt ttatcgccct tcgagtattg tgagcgctta
    16021 cttagcaccg gccggcacag ggctcccctc gtgcgcatgg cccttcgaat ccgaggggcg
    16081 caatgtcacg atgatggcaa agctgaccac ggcaaaaaag gtcacggtaa tgcctgtaat
    16141 ccaccaggcc gagtaagaca gcgttggcgt gaaggactcg gcggtaaaat caggcagcag
    16201 gttgtaggcg tgaaaatact tccgcaacag cgatcgtacg gcacccatca gccccatggt
    16261 ccaaatggcg ctgaaggcca ggaagaccaa aacgaactgt gaggcgaaat cgatcttacc
    16321 ccacacaatg gtcccctgcg aaaccgcacg gttgtagatg acatagttca ccaccgtcac
    16381 gaagatcaag gtgaacgcgg cggagttttt cgccggcatc agtgcaagga aattccagtc
    16441 ggatggcaat tccaactccg caaccagctt ggccccagtg ggcacaaaac cgtgcggcgt
    16501 catccagatc gcattcccca cgacgaccat caagaagccg accttgatga ccgtgctgga
    16561 cgatactgtc agaggtataa atcgccccag aataacgggc gccaacagga gcggcacgag
    16621 gaacgccagc gatgtcgcat caggcactgg atagtcggtc aggaccttgg tcatgacgat
    16681 cggcagcaac accatcacga tcggcgcgat cacggtcata cggacccgct ccaccccctc
    16741 gatccgcttc atgctcaacc agatatagta attgctggcc aagaaaatca ggcccaccat
    16801 ggcgccctgc atttcgaaga acatcgagag ctggtcggcc atcatgtacg gacaaattga
    16861 ggcgtcgtag tcgcagagct cataggacag caaataaccc ataaacggca agaacaagag
    16921 cgcgccgaca ccgatcatgt tgccgacaaa ccccatccaa tcgtagtagg cccgctcttc
    16981 ttccttcttc gagcccatga acatgtaggc ggcaatcagg ccggcgatga atccgccgaa
    17041 cgtcacgtta ccgacaagcc tatggagatt aagcggcatc cagctatagt tgaacacctt
    17101 atcccacagg gaagcggtcg ccaggaattc cgccggcgag acgccctcgg ccctcacagg
    17161 ggtattcatg aaggaggtcg gaccgtcgat cacgaacagc gtgatcgaac cgatgagatt
    17221 caataacacg cccaacgcga tatggcgcgc cttcttctca cccttccacg catcccacgt
    17281 ataaaaatac atgtagagga tgatggtctc gccgataaac aacagcggat acaccaccgc
    17341 aaatatcagg aagaagtgat tgatgagcca ggtcgtgaac tgcggatagg ttgcgagcaa
    17401 cacgaagata aagagaccgc ccgttaatgc cgtcatgctg tagagaatga ccgtgacctt
    17461 ggttacctct ttcgccagcc ggtcatagcg tggatcctgc ttgcgatacc ccaaccattc
    17521 cgagatgacg acgaaaatcg gggcccccag aatgaatccc gcaaagagaa tgtggagctg
    17581 agcgacgatc cataccgccg tgcgattccc ggtataaggg aactccacgg ggggcgcact
    17641 gggagcctgg gcgtatgcca cacctcccaa tatcgccact agcgcgaaca taaccaacaa
    17701 acagcgctgc cagattttca tgatgtctcg ccgctcctcc tcattccaac cgattgaccc
    17761 atgactcgaa ccgaacccgg cgtgagttac ttcacctgat cagcaggtac ccaatccttc
    17821 ttgttggcat cccagccctt acctgccaaa ccgaaggaac gttcaaacgc caaaatcttc
    17881 caacgatcct cttcggacaa cttgctcttc cagggcttca tcttggtgtt gggaacgccc
    17941 tcagaaatac gccagaacca gaccgagtca gaatacaact tcatgcgatc gccattgcgg
    18001 aagtcccggg cccccgcctt gaccggcttg ccgtccttac catggcaact ggcgcaattg
    18061 acgtcggggt tcttgccgcc gatgtacaac tccttacctt cttcgatgat tttcggatcc
    18121 gtccaccaac cggcaggcat atgcttgtcg gcatactccg cgggagcggg tggcggcgga
    18181 actacggggc cttcgcctcc cccttcacca ccgccgcagg cggcgacgcc aaaaccgaac
    18241 ccaagaatgc cgaccatcag agccaatctc ttcatgcctg cttgtttcat aaaaaagctc
    18301 ccgctccttt cttcatgccg gcgccctctc aagcaccaac acccattacc atcgacagaa
    18361 aaaaaagacg cttccctgcg gcgcaacgag cagccacagc aaaagcgtca agactcacaa
    18421 tacaacgagg tcacgtttgg acttccccgc ctacttcttg ctaatcgatc cggcgaggaa
    18481 caatgcccca cggaccgtgc cctatttctg ccgcttcccc tgtcccttct ttcgccgttg
    18541 ctcttctcgc atccgcttgc gggagatctt tagcccaatc catcccacaa tagcaacagc
    18601 cgcgatcccc agcccgtaca acaaccactg cggaggacca agatcgcgct cacacccact
    18661 tccgaaatca acccgaagct tacgttccgt ctccagatcg ttcgggtcga actggatttt
    18721 ataattcttt tgctccccgc cgacttcaac taacagcggg tcgcccacat tatcattgtg
    18781 aagatgcagg gtgactttat agtacccgtc accatcggtc gtcacagtct ggccgaccgt
    18841 gatccgcgta tcttttacca gcacctcaac attggcgccg ggtcgaccgt ctttgccaca
    18901 cacaaaacct tcgacggtaa atcgatggtc ggctgcgtgg ctcgcgaaca agaccgacgg
    18961 cacaaacaac gtcgagatca ggccccaaac gacaacgact cgccaggcgg ttttccgact
    19021 aattgctctc cgccatcgca tacgtatgac ccgtgtcgga cttgttttcg gcctcacaaa
    19081 catcccgccc acagccatgc tacgagaggc ctcatcgaca cggacacgac ccaaatttga
    19141 ggccgattct tctagcatag ccccccctct ttgtcaacaa atggatggag ttaaacggag
    19201 ggagccacga cgaaatgcgc gatgaaaatt ggggacgcgt gaagggcaat taaagccgct
    19261 ggcgcagaac gagccggtac aagaaaacga acgacggtca ctcgcgcgcc agcaagctga
    19321 cggctcacag atggccgcgg gatttggctt ccgcagcggc gcgatcaata tccttccgtc
    19381 gttccgattc cttttttgcg ctccaggctt cccaggactt gcctgtggcc gtgtcctcac
    19441 cggtgaaggc gatcgcgacc atctcagaaa acggaatcgt cagcgttccg ctgctggtcg
    19501 ccggaaagat ttcaatggtg ggttgctcac ccgtcgcggt gcgattaaag aggtatcccg
    19561 tcacatgccc ccccgacttc aattccacgg tgatatctcc ccggtaatca aatgccagct
    19621 cgatcgcctc tgccagttcc gtgagggagg ccggccgaaa gacccggcct tcaagactgc
    19681 cggtctccgg cgcatggtac ttcgcgtcgg tcataagtat ctcttatcgt ctagagggtg
    19741 atgcgtgaag cgttcggccg gctcagcgac cgaaccgctt caccgctgca ggaatcacga
    19801 tattcttcgc cccggttagc gcgcattcgg caacagggtg atcttcacgg tcttggcaaa
    19861 gccggagaac gacccgaacg tatcctccac tgccgaagcc tcatatccgc aatgcaccat
    19921 gcaatccgcg cacttttcgt tgcgcccggt gccataggaa tcccaatccg tctgttccat
    19981 caactcccgg aagctcgaca catagccctc ctgtagcaaa taacaggggc gctgccagcc
    20041 aaaaacgttg tacgtggggt tcccccaggg tgtgcattga tactcgcgct tgcccatgag
    20101 gaactccagg aacagcggcg actggttgaa ctgccagccg cgcttccgat tgcccaaaat
    20161 cttggagaac aattccgtgg tacgactgcg cttgagaaaa tgctgttgat ccggggcctt
    20221 ctgatagcta tacccgggcg agatcatcat accctcgacg ccgagcccca tcatttcatc
    20281 gaaaaacttc cggacccgct cgggattcgc atcgtcgaac aacgtcgtgt tggtcgtgac
    20341 gcgatgcccc cgcttgagcg ccgccttgat cgccttcacc gccacatcgt agacgccgtc
    20401 ccggcaaact gccaggtcgt gctcatcccg caggccgtcc atgtgcacgc tgaacgtcag
    20461 aaatttcgac ggcggatatt cgtccagttt ccgttccatc aaaatggcat tcgtgcagag
    20521 atacacatac ttctgacgct ccacgagccc ccgcacgatc tcaggcatct cgggatggat
    20581 cagcggctca ccgcccggaa tactgatgat gggcgccgcg cattcgtcgg ccgcagccca
    20641 acattgcgcc ggggtcaacc gcttatccag gacatgatcc ggatattgaa tcttgccgca
    20701 ccccgcgcac gcgaggttgc aacgaaacag gggctcgagc atgagcacga gcggataccg
    20761 tttgaccccc cggagtttct gggtcaacac gtacttggtc actgtataca tctgggagac
    20821 tggaacagcc atccctctct cctattctgg tgaatgtgaa tgtaacgtac gactgagact
    20881 gccggccttc tagcagacaa accgcgcgga ttctaccatt gcgattgaaa aatcgtcaat
    20941 ttcgcgtcag tgcagaaacc tgacgaaccc gcacgcgata gtcgagaaaa aacgcgagga
    21001 agataccaat gaccaacgca agcaccccag ccaccgccac gttgagcaat atgcgcggac
    21061 gcgtccgctt gtcggccggc acagcctggt caagcacctg caccgtcggc gtgtcccgtg
    21121 cctcggcgat cttcgcctgc tcatgctggg acgtcagcag ggtgaagagc gtttcttgaa
    21181 ccttgacttg tcggaagagc cgaccaaact gtagggccaa atcaggcacg gccaccatcg
    21241 caggatgaag tcgttcgctc gacaacatgc ccttctcatc cttccccgat cccatagtag
    21301 ccagctgctt cttgagctcc ccgacattcg aacggattcg ggccagatcc ggattatccg
    21361 gcgacagata acctcccatc acttggagtt gaacttcttg cgccgtaatc tgcccttgaa
    21421 tcatggccgc cgcttcaatc atgacctttg cctgcgcctc gacggccaca gtcttgttct
    21481 tcgcctgaaa atcccgtaac gcctcctcag ccttggccat gctctccatc gtctcgttga
    21541 gccgacgttc cagaaaggcg cgattctgcc cggccttgtt gaccgtcacc gtccggttca
    21601 agcgatcgag attcgataca taaaagtttg ctatatccgc tgccacctgc gggtcctcat
    21661 cctccaccgc aactttaatc actttctcct tggtcaccgt gatccgcaga tggtcttcca
    21721 attctttcct ggtttcgacc atcgtctttt cccggtaacg atccatcaag ttgaatttgt
    21781 ggatcacgtc atccgccatc aggcgtgact tcaggataga gacaaagacg tccgtcggcg
    21841 tggtcggcag accaggcaaa ccaagaccaa gattctgcgc catgccaccg gcagccggtg
    21901 aagccaacaa cgcggccaga gccccgcctt ccttggattc cagctggggc agaacggaag
    21961 aggtggattc gtagatattg gggaggagga aactgaccac gagggagacg atcattccca
    22021 tacaacagag gccggcgacc accttccgcc gctcaaccag aacatcccag taatcaaaca
    22081 gattggttga ttggtcaacc gtgggcctct ctgccggatg gcgaggagga tccagtaggg
    22141 cgggggagct atctgaggac cagtccatct aattagaccg tatctcgtga aggcatctca
    22201 tcgttcaaga ccgacacatg tcatgtgtca ttcgtcaagc gttcaccgaa acgactcgtg
    22261 cgaatgagtt cactgagttc ccacgcatga cgcttgacgt atcacgattg acctgctacc
    22321 gctctatcgg cccaccacgg caataccagc cgcggtcagc gcgagactgc cgataatgct
    22381 cgccacagtc tgccacaagg ccagctgagg agggcgagcc tcgattttct gcggcacaac
    22441 aatggtatct ccagggcgca tttccttcgc agagaggtac gaggagtcgg tggtcccatt
    22501 cgctcgcatc acatacatct cacgcttatt ggcatcttct gtgatacctc cggcctgacg
    22561 caagtaatca ttcaactcca agcccggtcg atacactacg gtgctggggt ttttaaccga
    22621 accgataatg ccgacggttt gcggaggagt cgggattgtg atccggtcac gagactccag
    22681 aatgatatcg tcttctgtcc cttccaggcg ctcaatggaa tccaagctca caatcactcg
    22741 tcccaactca atacgtgacg cagtggcctc cagctgctgg agacggagtg ccagcacctg
    22801 ctgttccgcc atggtcccgc ctgcggcggc cacaacgctt aaaccggcag tacctgcggc
    22861 aatcccggct gactgcgccg tcagccgctg tcgctccgcc gcaataaacc ggtccagctc
    22921 cgcctgctgt ttcagcttca ccgattcacg cacgaggaca attccgttcg gaaacgcatt
    22981 ctgtgtgaat cccccggacc gtttcaaaac cgaactgagg cgctccccga tctcgatggt
    23041 aaagtaacct gcgcgccgca cctccccttc caccagcacc agcgcgggcg gacgcatctg
    23101 actcgcaatc acaacctgat ctaaccggtt caagacatgg ttgtcggact cccgcccttc
    23161 aaacaccgcc ttagggctaa atggaatcac cttggtgaga taggttgtcg ggtcggtacg
    23221 aataatctcc gccttgtcgc cataagcatc gaccgtgagt tgctcgggag acaaaatgtc
    23281 cttcacgtgc atcccggcgc gatactcgta tggtcccgga cttttcaccg cccccacaag
    23341 actcaccacg ttcaccacct gcgtgggtaa ggtcgcgata cgcacatagt cgccatccag
    23401 caggatcgga tcgccgccct cgacaccaag gcggcttttc tccggtccct gtgaggcaaa
    23461 ggcgcgtacc aggtccacgt cgatcatgtt ccgctcttgc agcgccggat cctggcgaaa
    23521 cagatggcac cgttgtcggt cggaaagcgg cgtgagacct ccggcaagag tcaggagctc
    23581 cgtcaagcga gagccgggtt tcatttcata aatagccggc cgttttaccg atccgctgat
    23641 ggccacaacc gtccccaatg gcgggaccag cacgacatcg cccgcttgca gtcgattgtc
    23701 ctgtcgtcga tccccctgga gtaagaagtc gtacaggtct aactgcccga ccgccttccc
    23761 ctcccgcatg atgcggatct gccgcaagga gcccgatcgc gacggaccgc aggccgcata
    23821 aatcgcattc gatgcggtgg ccagcgcgct cagttcatag gctccgggcc gtgctacttc
    23881 ccccaccaca tacaccttca tcgtgcggat acgggccatt gagacgtgta gttcgaaacg
    23941 cttcaagagc atacttaacc gagcatggat cgtacgctcg gcctgcgaaa atgtttggcc
    24001 tccgacagaa atggcgccga cttgcggaat caccaccatc ccatcccgct caacgggcgc
    24061 aatgtaactg cggttaaaat tttgatccgg cacgttccag atgtggactg cgagcgaatc
    24121 ctgtggcccg atcacataat ccggcccgac cggcacgtcc tggagtgagg tgaatccacc
    24181 ggcgtgcgcg tcaaaaaaat catagccgaa ctgcttcaag cgacttttga ccccctgcaa
    24241 caccgaaaat tgcgcaaaag cctcttccac cgaaaaatcc tggtgccgca acaattcggc
    24301 cgtgaatggc tttcgctcga cctgttgcct ctgttggttc tgctgatttt gagcctgcat
    24361 gccacccatg gcaccctgta cggccgattg cagcccctgc aactgggaca cacctttctt
    24421 ctcccgctcc acctggctct gaagctccaa gccgcgtttt tccttttctg cctgcaaacg
    24481 ttcgagttgc cggtcgcgct cttcctcttc ctgtctcatt cgctgttcga cggaagtcgg
    24541 caataagctg ttcgcggcca tgggccaata gtcattcaga tttggcaccg tgttttccgg
    24601 cgagaggtca ggtgccgttg ggatcgggca aggggcatgc tggggcacaa tcggttgcgt
    24661 cgccgtcgga ttggtcacaa tcgcctgccc gggaggagtc ggcaaaccgt agttcccaaa
    24721 tgggagaaat ggcgacattt ggttcccgag cgacggcccg ccggctgaag gtgcggacga
    24781 gggtggaagc acgggattcg tcagcgtctg cgccagcaga gaaggaggaa gaacgatgct
    24841 acccaccagc agccagatca ccacgcatat ccagcggctg ggcacagtgc catgactaac
    24901 ggaacgcact gactcgtcgg ttgaactatg catacggttg agaccgccct agcctgtgga
    24961 taacgactgt aggttctaat ccaaaatcac cggctcagtc aaacgagagc aaattcagta
    25021 cggctaaaat tgaacagcga cggccgtcca gagcaaatga ttcttcgctg tcacgccggc
    25081 ggaaaaggtt tcggcgaaag gtgtaatggc cgatacctgg ccgggattgg tcagccgctg
    25141 aaacgtgtat ccgcccatga attgcacatc tttggaatac caccaggtga gatccacggc
    25201 cgcctcccgt ttcttctcat ggaccggttg cccgcgatcc ctctcctgca ggttgaagtt
    25261 cgcgcccaac tgcagtgtgt cggtcagata tcgagtggtg cggacaaaca agtcggttcc
    25321 gtcggttccc atatggtgcc cgagcggaaa cccgcggtac cgcatgccgc tgatataggg
    25381 cgaattgttg taccagacct ggcgcagctc gggatgacgc cgccggccgt agtccgtgtc
    25441 ggcatactca atgcgcaaat ccagcgtatc cccctggaac acctgcggaa tgtagatccc
    25501 gcccagaaag gcggtgcggc ttggaacggg aagttgcgac cacttgtcct ccgttccggc
    25561 ctcgccgtag agctgcagcc cggccggaaa gggaatcaga tagggaatcg aaggaatccg
    25621 gagccggaaa tcggccatcg cctgctcatt cacatcccga ttgccggttt gattgggctc
    25681 cgagatatag gcatcgaaca ccactccggg gaatgactgg tcacgcccgc ggccgccgaa
    25741 ctgcgtcaga cgagtgagtc cgaactccag ccaggaagcc ggcaggtaac tgagccgcac
    25801 cccgaacagt ttcgcgcgcg ggaaatcccg attctcctcc aaccggccca agaacgcatt
    25861 gaccttccag tccccgagcc cgctgagaaa tcccggcaat tgaaacgggc gttccgttcc
    25921 gagcttgatc atttccatcg ggaacgcgtg atcggtcagc agtaacgatc cgtgatagcc
    25981 gggcccccac cactgcgtcc cccgccccac ttccagcgcg acattggcat aactcaattt
    26041 gaggctgaac tcgcgcaggt agaaattttc gctgttgttt gtcgcaccca atcccaagag
    26101 atggcgattg ctgatgaact tcggctgcac catgaccgcg gcccagtcgc tcaactccgc
    26161 ccatgcgcgc acatccgttt gattctgcac gccgttcacc gagtattccc cgcctcgatt
    26221 ttcgcgaaac cgcacggtct ggccgccgcc gatcgaggag gcatcgaact ccgacgtgac
    26281 acgggcgccg aaccgcaagg cgccggtttt gtcgtcgggg cgcgcacgga cggtacccag
    26341 gcgaatgagt tccgaacgga actccgcgag caatcgttcc aggagcggtt cggcaatcgc
    26401 ctcccgaccg tccaattcga cctgatccgc gcggactcgc tcgaccgccc gcgccacata
    26461 ctgggccgcc tgcatgcggc tgaacggttt cgcgccgatt aacgcgcggt cgatcacgcc
    26521 caatccgatc aagcgctcaa tggcgtcata actccagtgg tgcagcggca cattgctgga
    26581 ggccagggca atcccgttca gcaagagcca cagcgccagg ccgagactga gcgcgaatga
    26641 aaggcgacgg attttcacaa ggcaggccgg ataggagtag aaagggaaag ttcgagaatg
    26701 agccgtgcgg cctgaatctc cgacagagaa cggtgacgac gcatcatttc cgcctgagcg
    26761 gagcggtcgg cgcaaggccg gcgcccgtct ctatgccggc tggagactgc gcggggtctt
    26821 cggacaggag accatcggtc tcgcctcccc gctcctggat caacgatgtg ccggccgggc
    26881 agtaggtggg aaggatggcg cagaggagcg ccagcacctt cgacacatga ccctccgcag
    26941 cagactcctc cagtctggcc acgagcccgg acaactgagc aaaactacgc gcagggtccg
    27001 gccggataca gaggactttc tgaaccgtag tcgactccac cgtttcgtga ccggcaacca
    27061 gttcctccgt cagtttctcg cccggcctgc aaccgagata ggtcaaggca atgtctttgt
    27121 cggggaccag tccggacaga cgaatcagat tgcgcgccat ctccaccacg ctgatctgct
    27181 cgcccatctc caacacgaag agttcccctc ccctggccaa ccgtgcggcc tgaagcacca
    27241 gatgcacggc ctcggtcatc agcatgaaat agcggcgcat gttggggtcc gtaatggtca
    27301 ccggcccgcc cgctttgatc tgctccagaa agagcgggac cacactgcca ttgctgccca
    27361 acacattccc gaatcgcacg gtggcaaaca ccgtctggga cgttcgggcc agcctctgca
    27421 caagcagctc cgccactccc ttgcttgcac ccatcacact gaccggattc accgccttgt
    27481 cggtcgagat cagaatgaaa cggtcgactt catgccgatg ggcggcctcc gcgaccaccc
    27541 tggtcccgcc gacattgttc aagaccgcct cgcagggatt tccctccatg agcggcacat
    27601 gcttatgcgc ggcggcatga aacaccagcg tggggcgata ctcggcaaac aaccggttca
    27661 tctgaccgac ctcggtgacg tcgccgatta ccggagccac gaacgtctta ccggccgcca
    27721 ctaactcgtt cacaacggca aacagcccgt tctcataacg gtccagcaac actaacgatg
    27781 caggagccag cccggcgacc tgccggcata attccgcgcc gatcgaccct ccggctccgg
    27841 tcaccaacac ccgctccccc tgcaccaatg cgcgaagcgg ttcaggatcc agatcgaccg
    27901 gaacccggtc caggaggtcc tcgatggaca gattccgaat ctggctgacc tccacccggc
    27961 actgcaacag atcccgtaag ttcggcaggg tttgaatggg gagatggaac ggctccagca
    28021 ccttcaccac cgcccgaatc gcggcaggct tggcactggg catcgccacg agcaccacgt
    28081 cgggagcctg tcgggcgatg atgtgagaca gacaatcccg gtctcccagg actctcaccc
    28141 catgaatgcg gtgcccgact ttcaccggat tgtcgtcaac gaacccgatc ggacgatagc
    28201 cgtaggaagg atggttacgc atttcccgca cgatcatggc tccggcatcc ccggcgccga
    28261 caatcagcac ccgcttccgg tcggtccgaa accaacccgc ttcccgtatc agccggggaa
    28321 gcacccgcaa cccggccagc aggcaccaca gcagcaaggc gtcgatgaca aaaatggagg
    28381 atggataggc tcgcgtaccc agcccgaatc gcaccacccc ggcaaaagcc aacgagctga
    28441 cggcgatact cagcgcaaga ttacggaggt cccacagacc ggtgtaccgc cagatgccgt
    28501 catagagccg gaaggggtag aaggtgaacg ccctgatgat cagcagcagc ggaagcatcg
    28561 ccacgagcag gtcccattcc tgcacaggaa tgtttccatc gaagcgaagc agaaatgcca
    28621 cccagttcga aagcgcagcc agtcccaagt ggcccacaaa cagcaacagg cgccgctgaa
    28681 ccgcggagaa cgatggcaag aacggacgag ccacgagata ccccagctga gccaacttcg
    28741 tcggactcga cttcacaatt cgaattggaa ggataccttg tcttgaatca ccgaatccag
    28801 cccttggact cgccccaagg aggatctaaa ccagaagaga cggaaataaa atcagccagt
    28861 gacttccgaa gaaatcgagt cagatcagac aggtcggccg accgcaaaga ggattttgta
    28921 gctggtagcc ggttgttccg gagaaattga aacgggagga gcataaacta ccatgtcctc
    28981 atcaaccgca gcagggaagg ggacaagttt cccgagaaac agccgcttca gtacacgctt
    29041 ccccgccatg ctcgacggga tcagacgcaa ccggacagcg acacgcttga tccacgagag
    29101 acaggctgca gggatagagg ccggcgcaat ggagaaagcg ccgaagatgt caggctgaaa
    29161 gccggcctcc cgcagtaaag ccgcgagttc ccgggcagaa taatagcggc aactgcgagc
    29221 gttcggatta aactccggcc attcggggtt gaccgtgcaa agcagcagga cccctggagg
    29281 agccagcact cgccgacact ccttcaaaaa ttttccggcg tcttcaaaaa aatagagcgc
    29341 ctcataacag atgatgacgt cgcgggatgc ggtgtgcagg ggcaaggctt cggcctggag
    29401 tcgcacgagg ggcctcgtgc tctgcagatt acgcttggcc tgaagaagca aacggtccgt
    29461 cagatccccg ccgacgatcc catcggcatg ccgtcccaga tagccgagcc cgacgccagg
    29521 gccgcaggcg acttccaaca cccggcggcc tcgacaaaag tccaccgcaa agcggtagcg
    29581 cgtatagacc atggacaacg cctctggcgt cacccaatcg cccgcctttt ctgtcacagg
    29641 actgtagtcg gagcgggtca cggctgggtt gagaaaaacg cccggagcgc ggcgacgatg
    29701 agctcgacct gcacctctct agtttcagcg ctcatcggca gagacaccac ctcccggcag
    29761 atcgagcgag tttcagggac atccgggacc gccagtccca aaccttcgtg ttcccacatg
    29821 ggtttctgcc aatggaccag cgtctcgatt ccctgttcct tcagatacgc gcgcaattga
    29881 tcgcggacca tggtgcggat gacatagttc tggaagatgt ccttgcgccg cgtctcatcg
    29941 aattgaggaa ttcgcaattg tgtgattccg gacagacccc gatgataggc agcggcaatt
    30001 cgccgccggt gagcaatcca gtctggcaga tgacgcagct tcacgtccag aacagcagcc
    30061 tgcacgttgt ccaac
//
LOCUS       DAORQN010000007        28189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_111_length_28189_cov_22.450096, whole genome shotgun sequence.
ACCESSION   DAORQN010000007 DAORQN010000000
VERSION     DAORQN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111_length_28189_cov_22.450096"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..635
                     /locus_tag="PK614_01210"
     CDS             3..635
                     /locus_tag="PK614_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB80861.1"
                     /translation="MGGRELVVLNNINLHIKRGELIAIMGASGAGKSTLLQILGTLDR
                     PTKGTVSFDGRNLFTLTEQEQAEFRNKRVGFVFQFHHLLPEFSALENACLPAMIQKRD
                     MADVVGEATKLLGEVGLADRLHHKPGELSGGEQQRVSVARALMQQPDLVLADEPTGNL
                     DSHTGDALFTLLRQLNRSRGTTFVIVTHNDKLSSQADRIVSMQDGMIVSS"
     gene            777..1922
                     /gene="traF"
                     /locus_tag="PK614_01215"
     CDS             777..1922
                     /gene="traF"
                     /locus_tag="PK614_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HRB80862.1"
                     /translation="MKTSFAPYVRLTTLCLAILLPLQAAAVEFVFVGSRQMGMGGAGV
                     ATTSDSLATYWNPAGMAMSKKFDIRFQGSGQVVDRGDVFDSLKDINNLNLNDTSAGNI
                     ARLQQQIDRINRPGTNLTAAASGGLYVKGNFGEHALGFNVSDVATAGGFLRGPVGFTN
                     NGTNLSVNGQFAMNGLEVRQAALSYAYAFMDRMFSIGVTGKVIQGAAYTGSTNIRGAS
                     DDVRVFEDIGRAKISTAIGIDVGAMFRPSSWLRMGIVAKDINAPTFDAPNGDKFKLLP
                     QVRTGVAVNPYNSLTLSADVDITKNNTLTPGVYSQVLSLGAEQTILSELLSFRVGAFK
                     NMQDAKTPFIPTAGFGLRILALRIDIAGGYDFRDQAALASGSVALTF"
     gene            2056..2574
                     /locus_tag="PK614_01220"
     CDS             2056..2574
                     /locus_tag="PK614_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80863.1"
                     /translation="MWEGPAARGFYPKTMAILPISGTYDSAREDVEEVVAKVLIKSRR
                     VEKVVPPDQVTDTFQTTKDAFDALVTYFSRMETTGQSDKNSAIKIGHALGADSLLVVK
                     VNAWEYTREEGDNIARVGLSMRLVDAVNGAIVWKARHQVQESYLFIRPDMRDLATKLA
                     TDMIKYMPPEKR"
     gene            complement(2577..3650)
                     /locus_tag="PK614_01225"
     CDS             complement(2577..3650)
                     /locus_tag="PK614_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB80864.1"
                     /translation="MAVARRKVSRTNRKTTSKTASVAPRRKPTTRVPAAMGEERIIRT
                     LQQGLFDTLSGSEGEGRSPISVSAAFVQSFLQLTKVQAIAVYVRDEQTREMTCLAEAG
                     SSDASVAGKWREGLARAEQNAQVSSGDLYGLRLHRIGRMDGVVMFQTGKSQRLPSRKL
                     LTVLTAVEPWLAVLLDHARLTVKYAAKILRIQHMEQVSDLLNSSLAEEEKLRRALDAA
                     VRLVEAEAGALFLSAGDGALTLCTVAGERATGLSALHSSIATGVHRTGQAVLITRGEQ
                     DARLVAGQGWQAALPVASLVSVPVRMGTQAVGVLEVVNRRSGKPFSNWDVLELASLSN
                     QFGLAIDNLRRGTVGEGGPKRTG"
     gene            complement(3853..4653)
                     /gene="trpA"
                     /locus_tag="PK614_01230"
     CDS             complement(3853..4653)
                     /gene="trpA"
                     /locus_tag="PK614_01230"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRB80865.1"
                     /translation="MNRLDHTLGQLKAKGEKALITYIMAGDPSLQETEQLVLELERAG
                     ADVIELGVPFSDPIADGPVIQKAAERGLQSGTSLRRILESVRHLRTSTQIPIVLMAYY
                     NNIHAFGEAEFCRIAAEAGVDGLIVPDMPPDEAGPLRGPAGEAGLHLIFLLAPTSTAS
                     RRAYVAKESGGFVYYVSITGITGAKLQDVAGVRENVAKIRKHTKTPVAVGFGVATPED
                     AARVAQVADGVIVGSAIVRRVGEHGQDGRLVQEVGAFVRSLKEAMQPA"
     gene            complement(4819..6009)
                     /gene="trpB"
                     /locus_tag="PK614_01235"
     CDS             complement(4819..6009)
                     /gene="trpB"
                     /locus_tag="PK614_01235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRB80866.1"
                     /translation="MAIPDRHGRFGPYGGRYVPETLMPALLELEEVYQRTRRDKGFQA
                     ELKHYLKEYVGRPTPLYFAQRLTKRLGGAKIYLKREDLCHTGAHKINNAIGQALLARR
                     MKKPRLIAETGAGQHGVATATVAAMFGLECEIYMGTEDMQRQALNVFRMRLLGSTVTG
                     VDAGSRTLKDAISEAMRDWTTNVRTTHYVLGSVLGAHPYPMMIRDFQSVIGREARKQI
                     LAAEGRLPDCLIACVGGGSNAMGLFHAFVPDKKVRMVGVEAGGLGVESGKHAARFAGG
                     RPGVLQGTMTYLLQDENGQVNLTHSVSAGLDYAAVGPEHSYYKETNRAQYTSVTDDEA
                     LEAFDLLAREEGIMPALESAHAIAEVVKCAPKMKKSQILIVNLSGRGDKDVQQVARIK
                     GVTL"
     gene            complement(6057..6689)
                     /locus_tag="PK614_01240"
     CDS             complement(6057..6689)
                     /locus_tag="PK614_01240"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRB80867.1"
                     /translation="MAVKVKICGLTNAEDAAVAVEAGADAVGFVFHKKSPRCAGQAVV
                     KAIVKELPPFVLPIGVFVNEDAKVVRDIMDSCGLALAQLHGDETAAYCEMLGRPVLKA
                     IRLRDRRSFLALAELQGRAGVRGFLVDAFSPDAYGGTGQVADWALAAEAATVARILLA
                     GGLTPGNVAQAIEQVRPYGVDVSSGVEASPGRKDHDKVRAFVQAVRLVSR"
     gene            complement(6694..7008)
                     /locus_tag="PK614_01245"
     CDS             complement(6694..7008)
                     /locus_tag="PK614_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80868.1"
                     /translation="MKTRLSLGVMAALLTLAGCMNQEIHEYSPKWDAWMGSSKDDRIK
                     DMGIPTRCHSFKDGGEVCEWSVPQQDGRQDLIGLTFNAKGQACQWSYRGFYGMQKSKQ
                     SC"
     gene            complement(7005..7829)
                     /gene="trpC"
                     /locus_tag="PK614_01250"
     CDS             complement(7005..7829)
                     /gene="trpC"
                     /locus_tag="PK614_01250"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRB80869.1"
                     /translation="MILDRILEHKKAEIRHKNSRGYLADLKTRIRDAGPTLGFAVNLD
                     ARRTPTRPALIAEVKKASPSLGLLRPEFEQRFEPVAIAEAYREHGAAAVSVLTDKDFF
                     QGSLEYLSDVKRKVGLPALNKEFMVADIQFYEARAYGADAVLLIVAGLEKRQLIDFSA
                     LAKELSLDVLVETHHERELDTVLEWLPDVRLIGINNRDLKTFSTDLGVTLRLAKRIPG
                     DKLIVSESGIHKREDVTRLVEAGVHAMLIGESLIRAQDIGVKIRELLGDESNKEKP"
     gene            complement(7834..8850)
                     /gene="trpD"
                     /locus_tag="PK614_01255"
     CDS             complement(7834..8850)
                     /gene="trpD"
                     /locus_tag="PK614_01255"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRB80870.1"
                     /translation="MIKDAINTLAERSDLTEQEAETVMGEIMDGAATSAQIAGYLMGL
                     RMKGETVAEIAGSVQAMRARATKIRVRDSQVVDTCGTGGDRAHTFNISTTAAFVAAGA
                     GLTVAKHGNRSVSSKSGSADVLAALGVAIDLPPERVADCVNEVGIGFLFAPLYHSAMK
                     HCAQPRQELGIRTLLNILGPLTNPAGARIQVVGVFDADLTELLAKVLLHLGAQHCFVV
                     HGMDGLDEITVTDRTRISEGKAGVVSSYTIDPTEFGLTRVRPKELVGGSAEDNAAITR
                     DIFRGRKGPKRDIVCLNAAPALVAGRKAKTLQEGFQLAQQTIDSGAAMEKLDQLIAFT
                     KKAS"
     gene            complement(8937..9500)
                     /gene="pabA"
                     /locus_tag="PK614_01260"
     CDS             complement(8937..9500)
                     /gene="pabA"
                     /locus_tag="PK614_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRB80871.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVVVYRNDKISIQEIEALKP
                     RRLVISPGPCTPNEAGISVEAIRHFGGKLPLLGVCLGHQSLAVAFGGEVIRAERLMHG
                     KTSMVRHDGRTIFRNLPNPFEATRYHSLIVNRKNLPDCFEISAETAEGEIMGMRHKTL
                     GIEGVQFHPESILTTAGKELLRNFLKL"
     gene            complement(9593..11089)
                     /gene="trpE"
                     /locus_tag="PK614_01265"
     CDS             complement(9593..11089)
                     /gene="trpE"
                     /locus_tag="PK614_01265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRB80872.1"
                     /translation="MSRQQYSLTLDKFRALAAEGNLIPLYREILADYETPVSAFAKID
                     HGPTAYLLESVAGGENWARYSFLGSGSSAVIREEKGDLVLTRGKKNLRIQSRGNPLER
                     LRELMEEYRPVTVPGLPRFVGGAVGYFSYDMVRTFEELPALRKDSLGMPDFAFLLTDT
                     LLIFDNVSQKIKIVANAYLESTKERDIRDAYRHATARIEKMIARLKRPVRQPRQRRRR
                     KPITFTSNMNRADFEKMVTRTKEYIRSGDIVQAVLSQRWETQIHTTPFQLYRALRVIN
                     PSPYMYYLRVAGVELVGSSPETLVRCEDGQISLRPIAGTRRRGQTPEEDQDLARQLLA
                     DEKERAEHVMLVDLGRNDVGRVAARGSVKVESLMQVERYSHVMHIVSQVTGQLEKGKS
                     VYDVMRACFPAGTVSGAPKIRAMEIIEELEPTRRGPYAGAVGYFGFSGNMDMCINIRT
                     VVIKGRQAYIQAGAGIVADSISEHEYEETCNKARAMMKAIELAEQGLE"
     gene            complement(11110..11808)
                     /locus_tag="PK614_01270"
     CDS             complement(11110..11808)
                     /locus_tag="PK614_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRB80873.1"
                     /translation="MMSQRNSRMRHAVVVPIGLLVLSGLNMSGCVMSEKYEAEKARSL
                     NFQRLLAQEEKRSAELDSEVKRSKRELSEYEARNRELGAQVEAVRQQAAQIQEEAQAM
                     KEASLLEKRAHEDMKRLTTIPSKTKKAAAAAKKDFTAAVDEALKTDVSSELSLESAKD
                     AAKDTFGHEQAGADALGTASASATGNTHTVRSGETLYRIGQKYHVGVDQLRKWNNLDN
                     NTVVVGQKLIVSRP"
     gene            12049..13002
                     /locus_tag="PK614_01275"
     CDS             12049..13002
                     /locus_tag="PK614_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB80874.1"
                     /translation="MSPNTQPGTSRTVTIEGITLHLAQPMTMGQEWIGNREILKQLLA
                     CWLVIDERDLPLSPRITGQPGIGKTTLAMAGARERKQDLYVFQCTADTRPEDLLITPV
                     LAESGTISYHASPLVTAVLTGSICVLDEGNRMNEKSWASLAPLLDHRRCVESIIAGIL
                     INAHAEFRCCVTMNEDASTYEVPDYILSRLQPTLGMGFPAREDELAILRYHLPFAPAE
                     MLAMTVDFLQEAHQLSLEYSVRDGIHLLQYALKRRAQDPTHPLSADAAWRESLMKVLG
                     EEALDLPTQSRRRKRALGDQALPQGLGDFFFESDDPLHPGR"
     gene            13002..14900
                     /locus_tag="PK614_01280"
     CDS             13002..14900
                     /locus_tag="PK614_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80875.1"
                     /translation="MAVSSSTADSVFRLSPRIELFPLLHGSGDVAQEVRERLTDRRFD
                     CLAVPLPPSFEHPLEEAVLDLPTISVIVQPEHDQEGAATVNYVPVDPCQAVVMGIRVA
                     MGEGIPRAYIDRETAVVEPVPFVSPDAYALKRLSYATFAAAMVPTLTPPAEGSQQQAR
                     IAWMAFQLHQLEMEYEQILCLCHFADWPWIRQAYQQRTDYQIPARDFGRPERYLVEPA
                     SLYFVLGELPFLTELYERRRATVHSDRHLSIDGIKELLLETRTRWQTGHDAEGQTVPT
                     WVTPQLLQAYLQYVRNHALLDRRLTPDLYTLVLAAKQMAGDDFAVRLLETAKSYSYQD
                     IEQSHVPHLSAGLGRFALPDEQIVQAKNRLQGTPLAWRSLSLRPRPPRRTSRQWSYLW
                     NPHRQCSWPPEDSKIESFHTHVREQAKAIIGADLAKSEKFTTSMKDGIDIRESLRHWH
                     QRQKPGRPQRMEIYVKEIPPARGNVDTVIFLFDTPGDPEQYSWQATWYAEHAQESTLC
                     FYATPFLNNMIAPGIGQSRYGGALFIFPPRPIPDIWTDEALGFARTLEERLIAAGAVH
                     SRETHVALVTPVPPKARWRQIARQFDRRLVPIPLSRFSGQMIDRLRRFHVLNGHEIRS
                     FAAQFIRE"
     gene            14908..16920
                     /gene="ligA"
                     /locus_tag="PK614_01285"
     CDS             14908..16920
                     /gene="ligA"
                     /locus_tag="PK614_01285"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRB80876.1"
                     /translation="MPHQTAEQRIADLRRQIRHHDHLYYTKDRPEISDAEYDRLFREL
                     VGLETAHPDLVTGDSPTQRVGAPPLEELIKVSHEKPMLSLDSITDQDDVRAFDARMKR
                     ELETDQIVYTAEPKFDGLSVELVYDRGAFVRGATRGDGMVGEDVTINLRTIRALPLQL
                     DPQPSLPAHLVVRGEVYMRLDEFQALNRRMTERGEEAFANPRNAAAGSLRQLDSRITA
                     SRPLTLTCYDIMAISGTAPSTHWDELDALAAWGLPVPEHRQRCGSINEVLSFQQAIDA
                     MRDSLPFEIDGVVVKLDRRAWQDQLGSKSRSPRWAIAYKFAPRKELTMIQDIVVSVGR
                     TGTLTPLALLKPVEVGGVTISRATLHNADEVARKDVRVGDTVKVERAGDVIPAIAERV
                     PVPGETRQEPFVMPDHCPVCGSAVAREGAYFYCTGQTVCVAQLKGAIEHFASKSALDI
                     DGMGKKTIAQLVDVEFVQDLADLYNLTKEQILTLEGFADRSASLLLESIERSKSVSLD
                     RLLMGLGIRQVGQHIARVLAKQFGTLPKLMSATQEELLQVREIGPEISASLTSFFSEE
                     RNRAVIARLIERGLTIAPPSTENATGSQHLAGKTFVFTGGLEGYSRDQAKQLVERQGG
                     QVSSSVSKKTSFVVAGTDPGSKLDQARKLGVRVLTETEFTALVTPE"
     gene            complement(16895..18208)
                     /locus_tag="PK614_01290"
     CDS             complement(16895..18208)
                     /locus_tag="PK614_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRB80877.1"
                     /translation="MDIIVLIVLILLSAVISVVEVGFYSVNDTKLRALADTGSKRAEM
                     ALHLRTDPQRLLLTILVGDRLIDTATASLATIIALNRFGGQGLEGVLGEAFAVLVGIL
                     TFVLLVFADLVPKTLAAKYSVPVVLNMAYPAYAAQQVLTPIMFFVVPLIYKLTGGKGL
                     NVPFVTEEELKIMLDQSSKSGAIEAQEVKMIKNVFQLKDITAEDCMTPRIYMFSLDCN
                     QYLREAKELLFKSKYSRIPLYEGTLDNIIGILYKTKALTALAQGHTEMKLRDIAQPAL
                     FIPHTKSADDLMKQFQLDKRHMAIVVNEFGGVMGLVTLEDLLEEVVGEIVDETDITEE
                     LIKRIGKNQILVHGRTEVRKVNDFLKVDLGDDAVTISGLVQHELGRIPKVGEEVHIAN
                     CRLVIHEADPRVIKSVNIYKEEKHPITHEPIVDEHVSVTQASLER"
     gene            complement(18476..19183)
                     /locus_tag="PK614_01295"
     CDS             complement(18476..19183)
                     /locus_tag="PK614_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRB80878.1"
                     /translation="MPGEKRTSTSRLLLPLFAFLLTTWLSGCSGWSPARPSYPPGYPL
                     GFIERGSASWYGPGFHGNRTANGEVYDMHKLTAAHRTLPLGSVAVVRSLSTGRQVTVR
                     INDRGPFARGRILDLSLAGAQAVGMVGRGTDEIELRVISYEGRAGGMGVLRVQVGSFA
                     DPANARALVERLRSAYPGSKIVGVDLPDGRRYRVYAGQFQTEVAAEQAAAHLRRALET
                     DPFIVRDDAPGATTGNP"
     gene            complement(19192..19602)
                     /locus_tag="PK614_01300"
     CDS             complement(19192..19602)
                     /locus_tag="PK614_01300"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRB80879.1"
                     /translation="MPTTTQFVVSGQSKPGVLAEVAAVLGAAGVNIKAFSAPEVTGPG
                     KLRLIVADVDAARIALRKSKIKFREETALILSLENKPGALKQVADSLTRARINVKCGY
                     CTPSREGKRAIVVLTVSNTTKALGVLRTHSLDEF"
     gene            complement(19642..19920)
                     /locus_tag="PK614_01305"
     CDS             complement(19642..19920)
                     /locus_tag="PK614_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB80880.1"
                     /translation="MVTVDQQAVAGEWQARGFSCDLWVDPPGQVWEDYRHATDELVMP
                     VEGRLELEFGGRCVRPEPGEEILIPAGVSHTVRNIGGTSVKWLYGYSR"
     gene            complement(19937..21049)
                     /gene="fbp"
                     /locus_tag="PK614_01310"
     CDS             complement(19937..21049)
                     /gene="fbp"
                     /locus_tag="PK614_01310"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HRB80881.1"
                     /translation="MAAESTQTITLSVIKADIGGFVGHSAMHPALMDCAKEKLAAAKT
                     SGLLVDYHVSACGDDLQLIMTHRHGVDHERIHRLAWDTFEAGTAVAKELHLYGAGQDL
                     LADAFSGNVRGQGPGVAEMEFVERKSDPVLIFMADKTSAGAWNLPLYKMFADPFNTAG
                     LVIAPTLHQGFRFEVHDIYEHKKISFDCPEELYDMLMFIGSPGTYTIKYVYSRADGTI
                     AAASSTERLSLIAGKYVGKDDPVMIVRAQQNFPAVGEVLDPFRYPWIIEGWMRGSHYG
                     PLMPVSVKQATPTRFDGPPRVACLGFQLADGKLVGPRDMFDDPSYDEARRDANRIADV
                     LRMHGPFEPHRLPLDDMEYTTMPQIMKKLEGRWTKV"
     gene            complement(21295..21687)
                     /locus_tag="PK614_01315"
     CDS             complement(21295..21687)
                     /locus_tag="PK614_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80882.1"
                     /translation="MGNFIFGAVIAVLGLAYVGGWDPGNEAVGHIVEVHQVADVGLIN
                     GEPDAGVRVMTTIKNEGKEGFLRVKARLSSSDGEWTREQKLHFDSGESKDLAWFFSEP
                     SVNASNVQSWVEVSPGVRSVQESKRKTI"
     gene            complement(22141..25038)
                     /locus_tag="PK614_01320"
     CDS             complement(22141..25038)
                     /locus_tag="PK614_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB80883.1"
                     /translation="MGMVMRSRWVLGLLATVALWCGGGQALADEPPSNPILRVETGMH
                     QAKINRIAVDAANRYLVTGSGDKTVRVWDLHTGTLLRTLRLPIGMGNEGEVNAVAISP
                     DGGTVAVGGWTGLDFDGSVSIYLFDRASGRMTKRLTGLPNVIGHLAFSPDEQTLVATL
                     GGKHGIRVYRTSDYAVIGEDRAYGDTTYGAAFDRQGRLVTTSYDGFIRLYDAKLQLLA
                     KQQAPGGSQPFGVAFSPAGLQVAVGFKDSTKLAVLSGQDLSFQFAPDTRKVTNGSFSS
                     VAWSADGHRLYAAGSGSNGTVWGWAKSGRGPAGVVPTGSVTIFSLVPLQDGGVVFGAA
                     DPAHGVLDAQGHRTFFQGPAIADYRSIFSSAWRLSPDGTTIEYGYEYGGKTPGRFAVV
                     VRALTVGQSSAGEGLALPKTDAAELTVTDWKDTASPKLNGKPLALEQRETSRCLAIAP
                     DEQGLLLGTEWYLRFFDRAGQERWEVLAPGVAWGVNIARNGRVAVAAFSDGTIRWYRL
                     SDGQELLAFFPHANRKDWVLWTPTGYYDASPGGEALIGWHVNHGKDQAADFFPAAKFR
                     STYYRPDIVAKVLGTLDEGAAVRLANSESGRKVQTVALTEQLPPVVTILSPGEGAMVS
                     TSEVTVRFTVRSPSGEAVTGVKALVDGRPVSVARDLKVTAKAQAESDVQELQVTIPER
                     DAEIAILAENRYSTSVPAVVRVKWAGRGQGQADAFVIQPRLYILAIGVSQYQNKDLTL
                     GFPAKDAQDFVAVLHSQKGRLYRDVTAKVLTDGEATKDDILDGLDWLRKETTSKDVAM
                     LFLAGHGVNDSNGIYYFLPANANPDKLLRTGVVFSDIKNTLASIAGKTLFFVDTCHSG
                     NIMGARRGVADINVVVNELASAENGAVVFASSTGKQYSLEDQVWGNGAFTKALVEGLD
                     GKADYTGKGTISINMLDLYLSERVKQLTDGKQTPTTTKPNTVPDFPIALRR"
     gene            complement(25050..27881)
                     /locus_tag="PK614_01325"
     CDS             complement(25050..27881)
                     /locus_tag="PK614_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRB80884.1"
                     /translation="MGNCRGTIVGFAMLAGTGLFLVTGCTTAPNSGAKDPLPLPSTSA
                     LSHEDQAAIKADMEAAQEELRTGKPAPLPKRTPPLNEPKPPLPSNHASQKTVRCESSV
                     PPAWVRQTAPWQAMGQSTVSQEEADRHARLEVVKSLEVKIDAVDRSSQQETSAEGFSY
                     SASSEVMETVQIAISGLDIVDRYADPCGAHFFALARLDRDRAVNAWGVELENLQARLG
                     ELSRHATSLQEQGEALRALGVWYQLLDLDATARQLDRRLRYLAAQQAKGESRAVLVEQ
                     ARRQFESLLASLKLVKAAGDEQRAVIAPQLGAPLVVRAMAGDHAIQNLPIQFRIEAGK
                     AEVDAHSVTDSQGLARATVHHIQAEDREATIIVRVSLEEALADYPEALRKRVLARQDQ
                     LMARFTMRSPVYHHLARLRELTDEAVRAKKVAAQHEAQGDVLRVLLSLAQLREAQESW
                     AKVADHLNGTDSEQRKGLAGPGDAGATHQEIQTVLGSLQVKKISGDQQRAFPGQPLAQ
                     PLRMQIVGILGGKEQGVAELPLQLSFEKGQGELDRLLRTDAAGHVDASVRKVEPSEHS
                     VTVLARVALDQLALDLTSSLRDQLARHFDAQAIRFTLIPPATSGDSSLLGKALYALAH
                     KLAQKVNTSEKVLTVVREFVEARSSRRFAISGRFESGLASALSGFGTLRILEVSPGAM
                     QTKNDAVTQAEAELVGVYERDPAGGVWLSAKLIRLPNHVTEAWEESTIARTAFSDEDL
                     RELGLLQAPPKGIVSSIVPTPSHDQNFHQWVEAFWDLRNPYGFHTELVAERKEYRIKE
                     AATFRFRTTRDCYLTVVNIGASGQVNLLLPNRWRPTPQDTLVRSSDGWVSVPSAIEDY
                     QFEVFEPLGTERVKAVCSTRPLRLFENANFKNGLFTLSPTTAGLMLNTTAMRPEEWSE
                     VHASVITLGADQTETQGLRGLKSRGLVTK"
BASE COUNT         5726 a         9179 c         7684 g         5600 t
ORIGIN      
        1 cgatgggcgg gcgggaactc gtcgtgttga ataacatcaa cctgcacatc aaacgcgggg
       61 aattgatcgc gatcatgggc gcctccggag ccggcaagag caccctgctg caaatcctcg
      121 gaaccctgga tcgcccgacc aaaggcacag tatcattcga cggccggaat ctttttacgc
      181 tcaccgagca agagcaggcg gaattccgca acaaacgtgt cgggttcgtg tttcaattcc
      241 accatctgtt gccggaattt tccgccctcg aaaatgcctg cctcccggcg atgattcaaa
      301 agcgcgatat ggccgatgtc gtcggcgaag cgacgaaact tctcggcgag gtcggtctgg
      361 ctgatcgact ccaccacaaa ccgggcgaac tctcaggcgg cgagcaacaa cgtgtctccg
      421 tcgcgcgcgc cttgatgcag cagcccgatc tggtgctggc cgatgaaccg acaggcaacc
      481 ttgactctca caccggcgac gccctcttca ccttgctccg tcaattgaac cgctcacggg
      541 gaactacgtt cgtcatcgtc acccacaacg acaagctctc atcccaggcc gatcgtatcg
      601 tctccatgca ggacgggatg atcgtgtcct cataggccga taccatcagc gatggcccgc
      661 gccggctcac gcggcacggc tgagtcgtcc gaccttgatt gggatttctt gacggcgtca
      721 gcatcttttc gtagactaac ccgtcctcgt actgaagtgg gagttcgacc tggtttatga
      781 agacatcgtt cgcaccgtac gtccggctca cgaccctatg tctggccatc ctcctgcctc
      841 ttcaggcggc ggcagtggaa tttgtgtttg tcggatcgcg ccaaatgggc atgggcggag
      901 ccggagtggc cacgacatcc gactccctcg ccacctattg gaatccggcc ggcatggcga
      961 tgagcaagaa attcgatatc cgttttcagg gcagcggtca ggtcgtcgat cgaggcgacg
     1021 tgttcgattc cctgaaagac atcaacaacc tgaatttgaa cgatacgtcg gccggcaaca
     1081 tcgcccgcct ccagcaacag atcgatcgca tcaaccgtcc cggcaccaac ctcaccgccg
     1141 ctgcctccgg cggattgtac gtcaaaggca acttcggcga acatgcactg gggttcaacg
     1201 tctccgacgt cgccaccgcc ggcggcttcc tgcgcggccc ggtcggattt acgaataacg
     1261 gcaccaatct cagcgtgaac gggcagttcg ccatgaacgg gctggaagtc cgccaagccg
     1321 ccctctccta cgcctacgcc ttcatggacc ggatgttttc gatcggcgtc accggcaagg
     1381 tcattcaggg cgcggcctat acgggatcca ccaatatccg aggcgccagc gacgacgtga
     1441 gagtattcga agacatcggg cgcgcgaaga tctcgaccgc gatcgggatc gacgtgggag
     1501 cgatgttccg gccttcgtcc tggttgcgga tggggatcgt ggcgaaggac atcaacgcgc
     1561 cgaccttcga tgcgccgaac ggagacaaat tcaaattgct cccgcaggtc cgcaccggcg
     1621 tggccgtcaa cccctataac tcgttgacct tgtctgccga tgtggatatt acgaagaaca
     1681 atacgctcac cccgggcgtg tacagtcagg tcctgagcct gggcgcggag cagacgatcc
     1741 tgtccgaact cctgtctttc agggtcggag catttaagaa tatgcaggat gccaagacac
     1801 cgtttattcc gacggcaggc ttcggacttc gcatccttgc cttgcgcatc gatattgccg
     1861 gcgggtatga tttcagagac caggctgccc tggcatccgg gtccgtggcc ttgaccttct
     1921 aaggttacgt tcgcactgaa tcaatctgct acacttgtta tatcaaccat gacacgacac
     1981 acagtacgca ctatcagtgt tgttttctgc ctcgtgatga ccggcctact ggccggctgt
     2041 ggaggtttgc aagaaatgtg ggaagggccg gccgcgcgcg ggttctaccc caagaccatg
     2101 gctattctcc cgatttccgg cacctacgat agcgccagag aagacgtcga agaagtggtg
     2161 gcgaaagtgc tgatcaagag ccgtcgcgtc gagaaggttg tgcctcccga tcaggtcacc
     2221 gataccttcc agaccacgaa agatgcgttc gatgcgctcg tgacctattt ctcacggatg
     2281 gaaacgacgg gccaatcgga caagaattcc gcgatcaaga tcgggcacgc gttgggtgcc
     2341 gactctctgc tggtggtgaa ggtgaatgcc tgggaatata cgagagaaga aggcgataac
     2401 atcgcccgcg tgggcctgag catgcggctg gtcgatgccg tgaacggcgc cattgtctgg
     2461 aaagcgcgcc accaggtgca ggaaagttat ctcttcatcc gtccggacat gcgggatctt
     2521 gccacgaagc tggcgacgga catgatcaag tacatgccgc cggaaaaacg atagcctcag
     2581 ccggtccgtt tcggcccgcc ctccccgacg gttcctctgc gcagattgtc gatcgccagc
     2641 ccgaattggt tcgagagact cgccagttcc agcacatccc agttgctgaa cggcttgccg
     2701 cttcgtctat tcaccacctc caacacaccc acggcctgcg ttcccatgcg gacaggcaca
     2761 gagacgaggg acgccaccgg caaggcggct tgccatccct gaccggcaac caatcgggca
     2821 tcctgctctc ctcgcgtgat caataccgcc tgacctgtgc gatgcacgcc agtcgcgatg
     2881 gacgagtgaa gggccgagag acctgttgcg cgctcgccgg cgaccgtgca gagtgtcaat
     2941 gctccatcac cggcactgag gaacaacgcc ccggcctccg cttccaccag gcgaaccgcg
     3001 gcatccaacg cccggcgtaa cttctcctct tcggccagcg acgaattcaa aagatcgctc
     3061 acctgctcca tatgctgaat ccgaagaatc ttcgccgcgt acttgacggt cagtcgcgca
     3121 tggtccagca ggaccgccag ccagggctcg actgccgtca acacagtcag caacttccgc
     3181 gagggcaacc gctgggactt cccggtctga aacatcacga cgccgtccat ccggccgatc
     3241 cggtgcaatc gtaaaccata caagtcgccg ctgctcacct gtgcattctg ttccgccctg
     3301 gccaggccct cccgccattt cccggccaca gaggcatcgc tgctcccggc ctccgccaga
     3361 caggtcatct cgcgggtctg ctcatcacgc acgtacacgg cgatcgcctg aaccttcgtc
     3421 agctgcagga acgattggac aaatgccgcc gagacgctga tgggtgaacg gccctcccct
     3481 tcggaaccgg acagggtatc gaacaatccc tgttgcaggg tgcggatgat gcgctcttca
     3541 cccattgccg caggcacccg agtcgtaggc ttgcggcgtg gagcgaccga tgcggttttc
     3601 gatgtcgtct tgcggttggt tcggctgacc ttacgacgag ctacggccat ggcgtctcct
     3661 catatgaagc gtcaaacgtt gcacagcagg gaacgtctcc gctgccacga attccttgga
     3721 tcaggacaat acgcgaccag gagccgccgt ccttgtctga acgaccgggc ggacagggct
     3781 ccacagaaat aatgtacgaa gatgtcggct gaaaagttga acagaatact gaggggggaa
     3841 aagacctgcc ggctaggccg gctgcatcgc ctccttgagg gatcgcacga acgcacccac
     3901 ttcttgaacg agtcggccat cctgcccatg ctctccaaca cgacgcacaa ttgcgctgcc
     3961 cacgatcacg ccatcggcga cctgagccac ccgcgcggca tcctccggag tcgccacgcc
     4021 gaatcccaca gccaccgggg tcttggtgtg ctttctgatc ttggcgacgt tctcgcgtac
     4081 gcccgccacg tcctgtagtt tagccccagt aatgcccgtg atggaaacat aatagacaaa
     4141 tccgccggac tccttcgcca cataggcccg cctcgacgca gtgctggttg gagccaacag
     4201 aaagatcaga tggagtccgg cctcaccagc cggaccacgt aacggccccg cctcatccgg
     4261 cggcatatcc ggcacgatca atccatccac accggcctcc gcagcgatcc ggcaaaattc
     4321 cgcctcaccg aacgcgtgga tgttgttgta ataggccatc aagacgatag ggatctgggt
     4381 cgaggtccga agatgccgca ccgattccag aatccttcga agcgacgtac cgctttggag
     4441 accacgctcg gctgccttct gaatcaccgg tccatcggcg atgggatcgg aaaagggcac
     4501 ccccaattcg atgacgtcgg cacctgcccg ctccaattcg agcaccaact gctccgtctc
     4561 ctgcaacgac gggtcccccg ccatgatgta ggtgatcagc gccttctctc ccttcgcctt
     4621 caactgcccc aatgtgtgat ccaaacgatt catatggata tacctatgcc tctcgctcgt
     4681 ccttccaatc aattgcagga ggttcaaaac ggtcatccag cgcggccgca gcaagcgcgg
     4741 aggtgaggcg tactcctgca gtacgtcgaa cctgtgcgcg ccgcgagaac aaagctggag
     4801 gccgttttca acctcctgct acagcgtcac gcctttgatt ctcgccacct gctgcacatc
     4861 cttgtcgccg cgcccggaca ggttcacgat caggatctgg ctcttcttca tttttggcgc
     4921 gcacttcacc acttcggcga tggcatgggc gctttccagc gcgggcatga tcccctcctc
     4981 ccgcgcgagc agatcgaacg cctccaacgc ctcgtcatcc gtgaccgacg tatactgggc
     5041 acgattcgtt tccttgtagt aactgtgctc cggccccacg gccgcatagt ccaagccggc
     5101 cgagacggaa tgggtgagat tcacctgacc gttctcgtcc tgcagcagat aggtcatggt
     5161 cccttgcaac acgccggggc gaccgcccgc aaaccgggcc gcatgtttgc cgctctcgac
     5221 acccagcccc ccggcttcca ctcccaccat cctgaccttc ttatccggaa cgaaggcgtg
     5281 aaacagcccc atcgcattac tgccgccgcc gacacaggcg atcagacaat cgggcagacg
     5341 cccttccgcg gccagaatct gtttgcgggc ttcacggccg atgacggact gaaaatcccg
     5401 gatcatcatg ggatagggat gcgcgccgag gacggaaccc agcacatagt gggtcgtccg
     5461 cacgttggtc gtccaatccc gcatcgcttc actgatggcg tccttcaacg tccggctgcc
     5521 ggcatccacg cctgtgaccg tcgaacccag gagccgcatc cggaacacat tcagcgcctg
     5581 ccgctgcatg tcttccgtgc ccatgtagat ttcacattcc aggccgaaca tggcagccac
     5641 ggtggccgtc gccacgccgt gctgacccgc gccggtctct gcaatgaggc gcggtttctt
     5701 catgcgccgg gcgagcagcg cctgcccgat cgcattgttg atcttatggg cgccggtatg
     5761 gcacaggtct tcgcgcttga ggtaaatctt cgcgccgccc aatcgtttcg taaggcgctg
     5821 ggcaaaatac agcggcgtcg gccgcccgac atattctttc aaatagtgct tgagttcggc
     5881 ttgaaacccc ttatcccgtc gtgtgcgttg gtacacctct tccaactcca gcaaggccgg
     5941 catgagagtt tcagggacat accgtccgcc gtaagggccg aaccgcccat gtcgatctgg
     6001 tatcgccatg tgaattcctt cagctagtcg tgaaaaatga aagtatagac gctgacctag
     6061 cgggagacaa gcctgacggc ttgcacaaaa gcccggacct tgtcatggtc tttcctgcct
     6121 ggcgaggcct cgactccgct gctgacatcg acgccgtagg gccgcacctg ttcgatggct
     6181 tgcgcgacat tccccggagt cagtcctccg gccaggagaa tacgcgccac cgtggccgct
     6241 tcagcagcca gcgcccaatc ggccacctgt cccgtgccgc cataggcgtc gggtgaaaag
     6301 gcatccacga gaaatccccg cacgccggcg cgcccttgca actcggccaa ggccagaaac
     6361 gagcgtcgat cccggagacg aatcgccttc agcaccggcc gccccagcat ctcgcaatag
     6421 gccgccgtct catccccatg aagctgcgca agggcaagac cgcaactgtc catgatgtcc
     6481 cgcaccacct tggcatcctc attcacgaac acgccgatcg gcaacacaaa cgggggcagt
     6541 tccttcacga ttgccttgac caccgcctgc cccgcgcacc gagggctctt cttatgaaac
     6601 acgaacccga ccgcatcggc accggcctcc accgccaccg ccgcatcctc ggcattcgtc
     6661 agaccgcaga tcttgacctt cacagccatg gcctcagcaa ctctgcttgc tcttctgcat
     6721 gccgtagaac ccgcgatacg accactggca ggcctgccct ttggcgttga acgtcaggcc
     6781 gatcaagtcc tgccgcccgt cctgctgggg gacagaccac tcgcacactt cgccgccgtc
     6841 cttgaaggaa tggcaccggg tgggaatgcc catgtcctta atgcggtcgt ctttgctgct
     6901 gcccatccag gcatcccact tgggggaata ttcatggatc tcctgattca tacaaccggc
     6961 caaggtcagc aacgccgcca tcacaccgag agaaagtcgc gtcttcatgg cttctccttg
     7021 ttggattcat cgcccagcaa ctcacgaatc ttcacgccga tatcctgagc ccggatcagc
     7081 gactcgccga tcagcatggc atggaccccg gcctccacca accgcgtgac atcctcccgc
     7141 ttgtgaatcc cgctttcgct cacgatcagc ttgtcgccgg gaatgcgctt cgccaaccgc
     7201 agggtcacac ccagatccgt cgagaaggtt ttgaggtcac gattgttgat gccgatcaga
     7261 cggacatccg ggagccattc caacacggta tcgagttcac gctcatgatg cgtctccacc
     7321 agcacatcga gcgacaactc tttcgccagt gcggaaaagt cgatcaattg gcgcttttcc
     7381 aaaccggcca cgatcaacag gaccgcgtct gcgccatagg ccctggcttc atagaactga
     7441 atatccgcca ccatgaactc tttgttcagc gcaggcaggc cgactttccg cttcacatcg
     7501 gagagatact caaggctgcc ctgaaagaaa tccttgtccg tcagcacgga gacagccgca
     7561 gccccatgct cccgataggc ctcggcgatg gccaccggct cgaagcgctg ctcgaactcc
     7621 ggccgcaaca ggccgagact cggagaggcc ttcttgacct ccgcgatgag cgcgggcctg
     7681 gtcggagtcc tgcgcgcgtc caggttgacg gcaaacccca gcgtcgggcc ggcatcgcga
     7741 attctggttt tcagatccgc gagatacccg cggctgttct tgtggcgaat ttccgctttt
     7801 ttatgttcga gaatccgatc gagaatcacg atgtcacgac gccttcttcg taaatgcgat
     7861 gagctgatcc agtttctcca tcgccgcacc cgagtcgatc gtctgctggg cgagttgaaa
     7921 cccttcctgc agcgtcttcg ccttgcgtcc ggccacgagc gccggtgcgg cattcagaca
     7981 cacgatgtcg cgcttcggtc ctttccgtcc ccggaaaata tcgcgcgtga tggcggcatt
     8041 gtcttccgca ctcccaccga ccagttcttt cggcctgaca cgtgtgaggc cgaattccgt
     8101 cggatcgatg gtataactcg acaccacgcc ggctttgccc tctgaaattc gcgtccggtc
     8161 cgtgacggtg atctcatcca atccgtccat gccgtgcacg acaaaacaat gttgcgcgcc
     8221 caggtggagc agcacctttg ccaacaactc cgtcaagtcg gcatcgaata ccccgacgac
     8281 ctgtatgcgg gcaccggctg gattggtcag ggggccgaga atattcagca gcgtccgaat
     8341 ccccagttcc tgccgcggct gggcacagtg tttcatcgca ctatgataga gcggcgcgaa
     8401 gagaaacccg atccccacct cgttcacaca gtcggcgacc cgctcaggcg gcaaatcgat
     8461 cgccacaccc agcgccgcca acacatcggc gctgccggac ttggacgaca ccgaccgatt
     8521 gccatgcttc gccacggtca gaccggcgcc cgccgctacg aatgccgccg tggtcgaaat
     8581 gttgaaggta tgcgctcgat cgccgccggt cccgcaggta tccaccacct gggaatccct
     8641 cacccggatt ttggtcgcgc gggcccgcat ggcctgtacc gagccggcga tctccgccac
     8701 ggtctccccc ttcattcgga gccccatgag gtacccggca atctgggcag aggtcgccgc
     8761 accgtccatg atctcgccca tcacggtctc agcttcttgt tcagtgaggt ccgaccgttc
     8821 tgccaatgta ttgattgcgt ctttgatcat gagaggcgcg atcagcgatc cgctgtcggc
     8881 aatcagtcgg aaggcttcat agagaacact gattgccggt ggccgacagc cgtggactag
     8941 agtttcaaga aattcctgag cagctccttg ccggcggtgg tgagaatcga ctccggatgg
     9001 aattgcaccc cttcgatgcc caacgttttg tggcgcatcc ccatgatctc gccttcggcg
     9061 gtctcggcgc tgatctcgaa acagtccggg agattcttgc gattcacgat gagtgagtga
     9121 tagcgggtgg cctcgaacgg gttcggcaga ttgcggaaaa tggtgcgacc gtcgtgacga
     9181 accatggacg tcttgccgtg catgagacgc tcggcgcgga tcacttcgcc gccgaacgca
     9241 accgccagcg attggtgacc gagacagacg cccagcagcg gtaatttgcc gccgaaatgc
     9301 cgaatcgctt ccaccgagat tcccgcctcg ttcggcgtgc aggggccggg cgaaatgacg
     9361 agccgacgcg gcttcaacgc ttcgatctcc tggatggaaa tcttgtcgtt tctgtagacc
     9421 acgacatctt cccccaactc gccgaaatac tggacgaggt tgtaggtaaa ggaatcgtaa
     9481 ttgtcgatca ttaacaacat atgaatacac ggagcgtatg gcagatggcg tatagcacgg
     9541 acaagaaggt ctcttcccgt cagctatatg ctattggcca tacgctcctc tcctattcca
     9601 atccctgctc ggcgagttcg atggctttca tcattgcccg cgccttgttg caggtttctt
     9661 cgtattcatg ttcagaaatc gaatcggcga cgataccggc tcccgcttga atataggcct
     9721 gccgtccctt gatcacgacg gtccggatgt tgatgcacat gtccatgttg ccggaaaatc
     9781 cgaaatagcc caccgcgccc gcatagggac cacggcgtgt cggctccaac tcttcgatga
     9841 tttccatcgc gcgaatcttc ggtgcacccg acacggtccc cgccgggaaa caggctcgca
     9901 tgacgtcgta cacggatttc cccttctcca gctgtcccgt cacctgcgat acgatatgca
     9961 tgacgtgcga ataccgctcg acctgcatca gcgactcgac cttcaccgag cctctggcag
    10021 ccacccgtcc cacgtcgttg cgtccaaggt ccaccagcat cacatgttcc gctcgctctt
    10081 tttcatctgc caacagctgt cgcgccaggt cctgatcttc ctccggcgtc tgcccgcggc
    10141 gacgcgtccc ggcaatggga cgcaacgaaa tctggccatc ctcacaccgc accaaagtct
    10201 ccggcgagga ccccaccaac tccactccgg ccacccgcag gtaatacatg tagggcgagg
    10261 ggttaatgac acggagggca cggtataact ggaacggagt cgtatggatc tgtgtttccc
    10321 atcgttgaga caggacggcc tgcacgatat cgcccgagcg gatatactcc ttggtccgcg
    10381 tgaccatctt ctcgaaatcg gccctgttca tgttggacgt aaaggtgatc ggtttccggc
    10441 ggcgtctctg ccgcggttgc cgcaccggcc gcttcaaccg cgcgatcatc ttttcgatcc
    10501 gcgcagttgc atggcgatag gcgtctcgaa tgtcgcgttc cttggtcgat tccaggtagg
    10561 cgttcgcgac tattttgatt ttctgcgaca cgttgtcgaa gatcagcagt gtgtcggtga
    10621 gcagaaaggc aaagtccggc atcccgaggc tgtccttgcg cagcgccggc agctcctcga
    10681 acgtccgcac catatcataa ctgaagtagc ccaccgcgcc gcccacgaac cggggcaggc
    10741 cgggcaccgt caccggccga tactcttcca tcaattcgcg cagccgttcc agcggattgc
    10801 cgcgactctg aatccgcagg ttcttcttcc cccgcgtcaa caccagatcg cccttctcct
    10861 cccggatcac cgccgaggag ccgcttccca ggaacgaata ccgcgcccaa ttttcaccgc
    10921 cggccacact ctccagcaga taggccgtcg gcccatggtc gattttggca aacgccgaca
    10981 cgggcgtctc atagtccgcc agaatctccc gatagagcgg aatcaaattg ccttccgctg
    11041 ccagggctcg aaacttatcc agggtgagag agtattgctg tctgctcata caggactgcg
    11101 accgccacgc tagggccggc tgacaatcaa cttctgcccg accacaaccg tgttattgtc
    11161 caaattgttc cacttccgca attgatccac tccgacgtgg tacttctgcc cgatgcgata
    11221 cagggtttca ccggaacgca ccgtgtgggt gttgccggtt gccgatgccg aagctgtacc
    11281 gagggcatcc gcgcccgcct gctcgtgacc gaatgtatcc ttggctgcgt ccttcgctga
    11341 ctccaaactc aactccgacg acacatccgt cttcaacgcc tcatcgacgg cggccgtaaa
    11401 gtccttcttc gccgcagctg ccgccttttt cgtcttggat ggaatcgtcg tcaggcgctt
    11461 catatcttca tgcgcccgct tctcaagcag gctggcttcc ttcatcgcct gcgcctcttc
    11521 ctggatctga gccgcctgct gacgcaccgc ttccacctga gcgccgagtt cccgattacg
    11581 ggcctcgtac tcactcagct cgcgtttgct tcgtttgact tcgctgtcca attcggcgct
    11641 gcgcttttct tcctgcgcca ggagccgctg gaagttcagg ctgcgcgctt tctcggcttc
    11701 gtatttctcc gacatcacgc atccgctcat attcaggcca ctcaatacga gcaacccgat
    11761 cggaactact actgcgtgtc tcatccgtga gtttcgctga ctcatcatgt cgctccttcg
    11821 ttgctgcctt ctccagggga ggctttcgaa tgccatcaag gtaacccgct catggcgcat
    11881 ccgcgtccga ttcgcagcct tgcgaaagcc ggtagaatac ggtccaaggc agcgaaagtc
    11941 aaagtgaaat gggctgcaga gacgtttgac cccctcccca cccctatgct accgttcgca
    12001 cgagattgcc gttccgccta ttctatccaa tcgtcggcac gaaagaccat gtcacctaac
    12061 acacaaccgg gcacctcccg caccgtcacc atcgaaggca ttactctcca cctggctcaa
    12121 cccatgacga tgggccagga atggatcggg aaccgggaga ttctgaaaca gttgctggcc
    12181 tgctggctgg tgatcgacga gcgtgacctg ccgctgtctc cccgcatcac cggtcagccg
    12241 ggcatcggca agaccaccct ggcgatggcg ggggcccgcg aacgtaagca ggacctctac
    12301 gtgttccagt gcacagccga tacccgtccg gaagacctgc tgatcacgcc cgtcctggcg
    12361 gaatccggca ccatcagtta ccacgcctcg ccgctggtca ctgcggtgct cacaggcagc
    12421 atctgcgtac tggacgaagg caaccgcatg aacgaaaaga gctgggcgtc gctggctccg
    12481 ctcctggatc accggcgctg cgtggaatcc atcatcgccg gcattctgat caacgcccat
    12541 gccgagttcc gctgctgcgt gaccatgaac gaagacgcgt ccacgtatga ggtgcccgat
    12601 tatattctct cgcgcttgca accgacgctg ggcatggggt tccctgcgcg cgaagacgaa
    12661 ctggcgatcc tccggtatca cctgcccttc gcgcccgcag agatgctggc catgaccgtc
    12721 gactttctcc aggaggccca ccagctcagt ctcgaatatt ccgtgcgcga cggcattcat
    12781 ctcctgcagt acgcgctgaa gcgtcgcgcg caggacccga cgcacccgct gtcggccgat
    12841 gccgcctggc gggaatcact gatgaaagtc ctcggcgaag aagcgctcga tctgccgact
    12901 cagtcacgcc ggcgaaagcg cgccctgggc gaccaggccc tgccgcaggg cctcggcgat
    12961 ttctttttcg aaagcgacga ccctctacat cccggccgat aatggcagtg tcatcctcca
    13021 ccgccgactc cgtcttccgc ctgtcaccac gcatcgaact gttcccgctc ctgcacggca
    13081 gcggcgatgt cgctcaggag gtgcgcgaga ggctcaccga tcgccgattc gattgcctcg
    13141 cggttccgct tccgccttcc ttcgagcatc cgctggaaga agccgtgctg gaccttccga
    13201 cgatcagcgt gatcgtccaa ccggaacacg atcaggaagg cgccgccacg gtgaactacg
    13261 tgccggtcga tccctgccag gcagtcgtca tggggattcg cgtcgccatg ggagaaggca
    13321 tccccagggc ctatatcgat cgcgaaacgg ccgtcgtgga accggtgccc ttcgtctctc
    13381 ccgatgccta cgcactcaaa cgcctctcct acgccacctt tgccgccgcg atggtaccca
    13441 cgctcacgcc gcctgcggag ggcagtcaac agcaggcccg catcgcctgg atggcgtttc
    13501 aactccacca actcgaaatg gagtacgagc agattctctg cttgtgccac ttcgcagact
    13561 ggccctggat tcgtcaggcc tatcagcaac gaaccgacta tcaaatcccg gcgcgcgact
    13621 tcgggcgccc cgaacggtac ctcgtcgagc cggcctcact ctattttgtc ttgggtgaac
    13681 tgccgttcct cacagagctg tatgagcgac gtcgcgcgac cgtccattcg gaccgccacc
    13741 tctccatcga cgggatcaaa gaactcctgc tcgaaacacg cacaaggtgg cagaccggcc
    13801 atgacgcgga gggtcaaacc gtccccacct gggtgacacc gcagttgctg caagcctact
    13861 tgcagtatgt gcggaaccac gcgctgttgg accggcgatt gacgcccgac ctctacacgc
    13921 tggtcctcgc ggcaaaacag atggcaggtg acgacttcgc ggttcgcctc ctcgaaaccg
    13981 ccaaaagtta ctcctatcaa gacatcgaac agagccacgt ccctcatctc tcggccgggc
    14041 tcggccggtt cgccctgccg gatgagcaga tcgtgcaggc gaagaaccgg ctccagggta
    14101 cgcccctggc ctggcgttcg ctctcgctcc ggccccgccc gccgcgtcgc acgagccggc
    14161 agtggtccta tctgtggaat ccgcatcgac aatgttcctg gccgccggaa gattccaaga
    14221 tcgaaagttt ccacacccat gtccgcgagc aggccaaggc catcatcggg gccgatctcg
    14281 ccaagagcga aaagttcacc acctcgatga aagacgggat cgacatccga gagagtctgc
    14341 gccattggca tcagcggcaa aagcctgggc gtccgcaacg catggagatt tacgtcaaag
    14401 aaattccccc cgcgcgcggg aatgtggata cggtgatctt tctgttcgac acccccggcg
    14461 atccggagca atacagctgg caggccacct ggtatgccga acatgcgcag gagtccacgc
    14521 tctgtttcta cgcgacaccc ttcctgaaca acatgatcgc gccgggcatc gggcaatcgc
    14581 gctacggcgg cgccctgttt attttcccgc cccgcccgat ccccgacatc tggaccgacg
    14641 aagcactcgg atttgccagg accctcgaag aacgtttgat tgcggccgga gcggtccatt
    14701 cacgtgaaac gcacgtggcc ctggtgacgc cggttccacc caaggcgcgt tggaggcaga
    14761 tcgccagaca attcgaccgg cgcctggtgc cgattccatt gagccggttt tcaggacaga
    14821 tgatcgaccg tctgcggcgg ttccatgtat tgaacggaca cgagatccgc agcttcgcgg
    14881 cccaatttat tcgtgagtaa ccagtccatg cctcatcaga cagccgaaca acgaatcgcc
    14941 gatcttcgtc gtcaaatccg ccatcacgat cacctctact acacgaagga ccggccggag
    15001 atttccgacg cggaatacga ccggctcttt cgcgagctcg tgggcctcga aacggcccat
    15061 cccgacctgg tcaccggaga ctcgcccacc caacgagtcg gcgcaccacc gctggaggaa
    15121 ctcatcaagg tttcccacga gaaaccgatg ctgagtctcg attcgattac cgatcaggac
    15181 gatgtccggg ccttcgatgc gcggatgaag cgggagctgg aaaccgacca gatcgtctac
    15241 acggcggaac cgaaattcga tggcctgtcg gtggaactgg tttacgaccg gggcgccttc
    15301 gttcgtggcg cgactcgtgg agacggcatg gtcggcgaag acgtgacgat caatttgcga
    15361 acgatccgcg cgttgccgct acagctcgat ccccagccct ccctgcccgc gcacctggtc
    15421 gtgcgcggcg aggtctacat gcgtctggat gaatttcagg cgctcaaccg tcgcatgacg
    15481 gaacgcgggg aagaagcctt tgcgaacccg cgcaatgccg cagcaggatc gttgcggcag
    15541 ctggacagcc gcatcaccgc gtctcgcccc ttgaccctga cctgttacga catcatggca
    15601 atatccggca cagctccatc cacccattgg gatgagctgg acgcattggc cgcctggggg
    15661 cttcctgttc ccgagcaccg gcaacgttgc ggctcgatta acgaagtcct ctcgttccaa
    15721 caggccatcg atgcgatgcg ggactcgctt ccgtttgaaa tcgacggcgt cgtcgtcaaa
    15781 ctcgaccgcc gggcgtggca agaccagctt ggcagcaaat cccgcagccc ccgttgggcc
    15841 atcgcctaca agtttgcacc ccgcaaagaa ctcacgatga ttcaagacat cgtcgtctcg
    15901 gtcggtcgga ccggcaccct gacgccgctg gccctgctga aaccggtgga ggtcggaggt
    15961 gtcacgatca gccgggccac cctccacaat gcggatgagg tcgcgcgaaa agacgtgcgc
    16021 gtgggggata ccgtcaaggt ggaacgcgca ggcgacgtga tacccgcgat tgccgagcgg
    16081 gtcccggtcc ctggagagac gagacaggaa ccgttcgtca tgccggacca ctgtccggtg
    16141 tgcggatcag ctgtggcgcg cgaaggggcc tacttctact gcaccggcca gaccgtctgc
    16201 gtcgcgcaac tgaagggcgc catcgaacac ttcgcatcca agagcgcgct ggatatcgac
    16261 gggatgggaa agaagacaat cgcccaactg gtcgatgtcg agttcgtcca ggacctggcc
    16321 gatctctaca acctgaccaa agagcagata ctcacactgg agggatttgc cgaccgatcg
    16381 gcaagcctgc tcctcgaatc gatcgaacgc agtaagtccg tctcactgga ccggctgtta
    16441 atggggctgg gcatcaggca ggtggggcag cacatcgccc gcgtgctggc gaaacagttc
    16501 ggcaccctgc cgaagctgat gtcggcgacg caggaggaat tgctgcaggt gcgcgaaatc
    16561 ggcccggaga tttctgccag cctcacgtcg ttcttcagcg aggaacggaa ccgggcagtc
    16621 atcgcgcgtc tgatcgaacg gggtctcaca atcgctcccc cgagcactga gaacgcaacc
    16681 ggcagccaac atctggccgg taagacgttt gtctttaccg gcgggctcga aggctacagc
    16741 cgcgaccagg ccaaacaatt ggtggaacga caagggggac aggtctcgtc gagtgtcagc
    16801 aagaagacat cgtttgtggt ggcagggacc gatccggggt cgaaactgga ccaggcccgg
    16861 aaactggggg tgcgggttct gacggaaacg gaatttaccg ctctagtgac gcctgagtga
    16921 ccgagacatg ctcatcgacg atgggctcat gcgtgatggg gtgtttttct tccttgtaga
    16981 tattgacgct cttgatgacc cgcgggtccg cctcatgaat gaccaggcga caattggcga
    17041 tatgcacttc ttcaccgacc ttgggaatcc tgcccagttc atgctgcaca agaccgctga
    17101 tcgtcacggc atcgtcgcct aaatccacct tgaggaaatc gttgaccttc cgaacctcgg
    17161 tgcgtccatg caccaggatc tggttcttgc cgatacgctt gatcaactct tcggtgatat
    17221 ccgtctcatc gacgatctcg ccgaccacct cttccagcag gtcttccaac gtgactagtc
    17281 ccatcacacc cccgaactcg ttgaccacaa tcgccatgtg gcgtttgtcc aactgaaact
    17341 gcttcatcag gtcgtccgcg gatttcgtat gcgggatgaa cagggccggc tgcgcgatgt
    17401 cgcgaagctt catctccgta tgcccctggg ccaacgccgt caacgccttg gtcttgtaga
    17461 gaatcccgat aatgttatcc agcgtgcctt cgtatagggg aatgcgcgaa tacttggact
    17521 tgaacaggag ttccttcgct tcgcgcagat actgattgca gtcgagcgag aacatgtaga
    17581 tacgcggggt catgcagtct tcggccgtga tgtccttcag ctggaagacg ttcttgatca
    17641 tcttcacttc ctgcgcctca atcgcgccgc tcttgctgct ctgatccagc atgatcttca
    17701 gctcttcttc cgtgacgaag ggcacgttga gccctttgcc gccggtcaac ttatagatga
    17761 gcggcacgac gaagaacatg atcggcgtga gcacctgctg ggccgcatag gccggatagg
    17821 ccatgttcaa caccacaggg accgagtact tggcagccag ggtcttgggc acgagatcgg
    17881 caaacaccag cagaacaaag gtcagaatac cgaccaggac cgcgaaggct tcgcccagca
    17941 caccttccag cccctgccct ccgaatcggt tcagcgcaat aatcgtggcc agcgatgcgg
    18001 tggcggtgtc gatcagtcga tctccgacca ggatggtcag caggaggcgt tgcggatcgg
    18061 ttcgaagatg gagggccatc tccgcccgct tgctgcccgt atcggccagc gccctgagct
    18121 tcgtatcgtt gacggagtag aatccgactt caacaacaga aatcaccgcg gacaacagaa
    18181 tgaggactat cagaacgata atgtccataa gacctatcga ggttagaggt gcagactcac
    18241 gttgaaggag tgaatggtgg aaccttggat cagtccgaag ccttgggttc gaactgcaag
    18301 acttgcccta tgtcgtgaga agacgaccag accgggtcct ctcgggtcac tttcatccat
    18361 cgcgtattac aattagcaca ggcagacagg cgcatcaagc attacaacac ttccatgccg
    18421 agcacagtta gtcagcatat cagtcattta gactatttac catgctcccg caccctcaag
    18481 gattccccgt agtcgccccc ggcgcatcgt cgcgcacgat gaatggatcg gtctccagtg
    18541 cccgtctcag atgcgcggcg gcctgctcgg ctgcgacctc cgtttgaaat tgtcccgcat
    18601 acactcgata ccggcgacca tccggaagat cgacgccgac gatctttgaa ccaggatagg
    18661 cactcctcaa ccgttcgacc aaagctcgtg cgttggccgg atcggcaaag gaaccgacct
    18721 gcacgcgcaa cacccccatc cccccggctc gtccctcgta actgatcacc cggagttcga
    18781 tctcatcggt gccgcgcccg accatcccga cagcctgggc tccggccaga gacaggtcca
    18841 gaattctccc gcgcgcaaac ggcccccgat cgttgatccg cacggtcacc tgccggccgg
    18901 tgctcaggga tcgcacgacc gccaccgacc ccagcggcaa tgtccggtgc gccgccgtca
    18961 atttgtgcat gtcgtagact tccccgttcg ccgtgcggtt gccgtggaag cccggcccat
    19021 accaggacgc gctgccccgt tcgataaacc ccaacggata accgggcgga tacgaaggac
    19081 gcgccggact ccagccggag cagccgctga gccatgtggt gaggagaaaa gcgaacaggg
    19141 ggagaagaag acggctggtg cttgtcctct tctcccccgg cataaggcgg attagaattc
    19201 gtcgagtgaa tgggtacgca gcaccccgag ggctttggtc gtgttcgaca cggtcagcac
    19261 cacgatcgcg cgtttgcctt cccgcgacgg ggtgcaatag ccgcacttca cattgatgcg
    19321 agccctggtg agcgaatcgg ccacctgttt caaggcgccg ggcttattct ccagactcaa
    19381 aatcagggcg gtctcttcac ggaacttgat cttggatttt ctgagcgcaa tacgagccgc
    19441 atccacatcc gccacgatca accgcagctt cccggggccg gtgacctcgg gcgcagagaa
    19501 cgccttgatg ttgactcccg cagccccgag cacggccgct acttcagcca gcactccagg
    19561 cttactctgt ccactcacca caaattgcgt cgttgtcggc atgggctcct ctccctctgc
    19621 tgagtcagtt cgtctcggat gttagcgact gtatccatac agccacttca cgctggtgcc
    19681 gccgatgttt ctgacggtat gggacactcc ggccggaatg aggatctcct cgcccggttc
    19741 gggcctgaca caccggcccc cgaactccag ttccaaccgg ccctcgaccg gcatcaccaa
    19801 ctcgtcggtc gcgtggcggt aatcctccca cacttgcccg ggcggatcaa cccaaagatc
    19861 acaactgaag cccctcgcct gccattcccc ggcgactgcc tgctgatcga ccgtgaccat
    19921 agttctccgc aaccagctac acctttgtcc agcgaccttc caacttcttc ataatctgcg
    19981 gcatggtcgt gtactccatg tcgtcgaggg gcaaccggtg cggttcaaag ggaccgtgca
    20041 tccgtaacac atcggcgatc cggttcgcat cccgacgcgc ttcatcatac gacggatcgt
    20101 cgaacatatc ccgtggcccg acgagtttgc cgtcagccaa ttgaaatccc agacaggcga
    20161 cgcgtggagg cccatcgaag cgggtcggcg tcgcctgctt caccgagacc ggcatgagcg
    20221 gaccgtaatg cgaaccacgc atccagccct cgatgatcca gggataccgg aacggatcga
    20281 gcacttcgcc caccgccgga aagttctgct gcgcgcggac gatcatcacg ggatcgtctt
    20341 tcccgacata cttgcccgca atgagcgaaa gccgctccgt cgacgaggcc gccgcaatcg
    20401 tcccgtccgc ccgtgagtat acgtacttga tcgtgtacgt acctggggaa ccgatgaaca
    20461 tcaacatgtc gtagagttct tcgggacaat cgaatgagat cttcttgtgc tcgtagatat
    20521 cgtgcacctc gaaccggaat ccctggtgca gcgtgggcgc aatgacgaga cccgccgtat
    20581 tgaacggatc ggcgaacatt ttatacaggg ggagattcca ggcacccgcc gacgtcttgt
    20641 ccgccatgaa gatcagcacc ggatcggact ttcgctccac gaactccatt tcagccacac
    20701 cgggcccctg cccgcgcaca tttccactga aagcatcggc cagcagatcc tgccctgcgc
    20761 cgtagagatg cagctccttc gcgaccgcag tgccggcctc gaatgtatcc cacgcaagac
    20821 ggtggatccg ctcgtgatcc acgccgtgac gatgcgtcat gatgagctgc aagtcatcgc
    20881 cgcaggccga cacatggtaa tcgacgagca gcccgctggt cttcgccgcc gcaagcttct
    20941 ccttcgcgca atccatcaag gccggatgca tggccgaatg cccgacgaag ccgccgatgt
    21001 ccgccttgat cacactcaac gtgatggtct gtgtgctctc cgctgccatg ttgcacctct
    21061 gggtgataaa gcctggttga tggcctctgc gccgaatctc cagcgtcggc cctggaacgt
    21121 cctcatctcg gaatgagcaa aggcaatgcc cgggcatgga ttgagcagac aggtagaata
    21181 ggccgaattt gcagcgtgaa ccgccgccgc gcgcaccaca cccgtggaca ggcgctacag
    21241 atttgcatgg aaacctgtag cagaaggcag caggccccat tcgtacctgc aatgtcagat
    21301 cgtctttctt tttgattcct gtaccgatct gacaccaggc gatacttcca cccaagactg
    21361 aacgttggac gcattgaccg aaggttcact gaaaaaccac gccagatctt tggattcacc
    21421 gctgtcgaaa tgcaacttct gttcgcgggt ccattctccg tctgaactgg acaatcgggc
    21481 cttcacgcga aggaaccctt ctttcccctc attcttgatg gtcgtcatca cgcgcacacc
    21541 cgcgtccggc tccccattga tgagccccac gtccgcgacc tgatgcacct ctacaatgtg
    21601 accaaccgcc tcattccctg ggtcccatcc gccaacatag gccaacccca gcactgcaat
    21661 gaccgccccg aagataaagt ttcccatgac gcacctccgt ttgtgagtaa cgattcgcaa
    21721 cctgtttcct taaggagcct tcatcctgac tcgtccccaa aactccaaag aatgccctct
    21781 cattcatgct gcagcgaacg cggcccatgt gtctcagagc caatgtctac aagatccagc
    21841 accgccagga tgaagattgc tccgccaaga atgagctcca gcatggtgtc ctccttttca
    21901 gcgacgcggt tcatacatga ggtatcgcaa ccccaatgcc agcgggcagc cctcaagaga
    21961 acgggagcag gatcagaact aactatccag aaaaacaatg agttagagaa aggaggggca
    22021 ggaatgatgc ggctggatta cacgaaggaa gatgaagacg aacctgagac ctccccgcat
    22081 cgtgaaggga caggtcgcgc cctgcaggga acaggtggcc ggagagggtc cgaaccaacc
    22141 ctagcgccgc agggcgatcg gaaagtccgg aacggtgttg ggtttggtgg tcgtgggggt
    22201 ctgcttgccg tcggtgagtt gcttcacccg ctcggagaga tacagatcca acatgttgat
    22261 gctaatcgtg cccttgccgg tatagtcagc cttgccgtct aggccttcca ccaacgcctt
    22321 ggtaaaggcg ccgttccccc agacttgatc ttcaagcgaa tattgcttgc cggtggagga
    22381 ggcgaagacc acggcaccgt tctccgcgct ggccagctcg ttgaccacga cgttaatgtc
    22441 cgcgacaccc cgcctggcgc ccatgatgtt gccggaatgg caggtatcca cgaaaaacag
    22501 cgtcttgccc gcaatggacg ccaaggtatt cttgatgtca gagaagacca cgccggtccg
    22561 tagcagcttg tccggattcg cattggccgg taaaaagtag tagatgccgt tggaatcatt
    22621 cacgccgtgg cccgcgagaa acagcatcgc cacgtcttta ctcgtggtct ctttccgtag
    22681 ccaatccaga ccatcgagaa tgtcgtcttt cgtcgcctcc ccatcggtga ggaccttcgc
    22741 cgtcacgtct cgatacaacc gacccttctg gctgtgcagc acggccacga aatcctgcgc
    22801 gtccttggcg ggaaacccca gggtcaaatc cttgttctgg tactggctga ccccgatcgc
    22861 cagaatatag agtctgggct ggataacgaa ggcatccgct tgtccctgcc cccgtcctgc
    22921 ccacttcacc cgcaccaccg cgggcacgct ggtgctgtaa cgattctctg ccagaatggc
    22981 aatctcagca tcccgctcgg gaatcgtgac ttggagttcc tgcacgtcgg actcggcctg
    23041 cgccttcgcg gtgactttga gatcccgtgc cacggagacg gggcgcccat ccaccaaagc
    23101 cttcacgccg gttaccgcct ctcccgaagg cgagcgtacc gtgaaacgaa cggtgacctc
    23161 agatgtggaa accatagccc cctcgccagg agaaagaatc gtgaccaccg gcggaagttg
    23221 ttcggtcaag gccacggtct ggaccttgcg cccgctctca ctgttcgcca ggcgaaccgc
    23281 cgccccctca tccaaggtcc ccaacacctt cgcgacaata tcgggccgat agtaggtgct
    23341 tcggaatttc gcggcaggaa aaaagtccgc cgcctggtcc ttgccatggt tgacatgcca
    23401 cccgatcaac gcttcgccgc ccggggaggc atcgtagtag ccggttggcg tccagagtac
    23461 ccagtccttt cgattagcat ggggaaagaa ggccaggagt tcctgcccat cgctcagccg
    23521 gtaccagcgg atagtgccgt cactgaaagc cgctaccgcc acccggccat tgcgggcgat
    23581 gttcacgccc caggccactc caggcgccag cacctcccac cgttcctggc cggcccggtc
    23641 gaagaagcgg agataccact ctgtgcctag gagcaaacct tgttcatccg gcgcgattgc
    23701 caggcaacgt gatgtctcac gctgctctaa cgcgagcggc ttgccgttga gcttgggtga
    23761 tgcagtgtcc ttccagtccg tcacggtaag ctcggcagca tcggtcttgg gtagcgccaa
    23821 gccttctcca gcactgctct ggcctacggt taaggcgcgc accaccacgg caaaacgccc
    23881 aggggtcttg cccccatatt cataaccata ctcgatggtc gtgccatcgg gtgacagacg
    23941 ccaagcacta ctgaagatgc tccgatagtc tgcgatggcc gggccttgaa agaaggtccg
    24001 atggccctgc gcatccaaaa caccatgagc tggatcagcg gctccaaaaa ccacccctcc
    24061 atcctgcaac ggaacgaggg agaagatcgt gaccgagccc gtcggtacaa cgccagcggg
    24121 accacggccg ctctttgccc aaccccaaac agtaccgtta ctgccgctcc ctgccgcata
    24181 gaggcgatga ccatcagcag accaggcaac gctggagaaa cttccgtttg tcaccttcct
    24241 ggtatcaggt gcgaactgaa acgagaggtc ctgcccggat agcacggcca acttggtcga
    24301 gtccttaaat cccactgcga cctgcaaacc ggccggcgag aaggcgacgc caaaaggctg
    24361 tgaaccacca ggggcctgct gtttcgccaa aagttggagc ttggcatcat agagcctgat
    24421 aaagccatca taagacgtgg taacaagacg cccctgccgg tcgaatgcgg cgccgtaagt
    24481 ggtgtctcca taggcacgat cctcaccgat caccgcataa tccgacgtgc ggtagacccg
    24541 gatgccgtgt tttcctccca aggtcgctac cagcgtctgc tcgtcaggag aaaatgccaa
    24601 atgaccaatt acattgggca ggccggtgag ccgcttggtc atgcgtccac tagcccgatc
    24661 aaagagatag atactgaccg agccgtcgaa gtccaagcca gtccagccgc ccacggcaac
    24721 cgtcccgccg tccggcgaga tcgccaccgc attgacctcg ccctcattgc ccataccgat
    24781 tggtaaccgc agggttcgca gcaacgtacc tgtgtgaagg tcccagaccc gcacagtctt
    24841 gtcaccagag cccgtcacga ggtagcggtt ggccgcatcc acggcaattc gattgatctt
    24901 ggcttggtgc atacctgtct cgactcgcag aatcggattg ctcggcggct catcggctaa
    24961 ggcctggcct cccccgcacc agagggccac agtcgccagc aggcccagga cccaccgcga
    25021 tctcatcacc atccccatga gtgatctcct cattttgtga ccaaccctcg cgatttgagc
    25081 ccgcgcaacc cctgcgtttc tgtctggtca gcacccagtg tgatcacgct cgcatgcacc
    25141 tcgctccact cttcaggtcg catcgccgtc gtgtttagca tcagtccagc ggtcgtcggg
    25201 gagagggtga agagaccatt cttgaagttc gcattttcaa acagccggag cggacgggta
    25261 ctgcacacgg ccttcacccg ttccgttccc aacggttcaa acacctcgaa ctggtagtcc
    25321 tcaatggcgc tcggcaccga cacccagcca tcactcgaac gaaccagcgt gtcctgaggg
    25381 gtcggtctcc agcgattcgg caacaatagg ttaacttggc cactcgcgcc gatgttcacg
    25441 acggtgagat agcagtcgcg cgtcgtgcgg aatcgaaagg tcgcggcctc cttgatgcga
    25501 tattcctttc gctcagcaac gagctccgtg tggaatccat agggattgcg cagatcccag
    25561 aaggcttcca cccactgatg aaaattttgg tcgtggcttg gcgttggtac gatcgacgaa
    25621 acaatgcctt tgggcggtgc ctgaagcagg cccagttcgc gcagatcctc atccgagaat
    25681 gccgtgcgcg cgatggtact ttcctcccac gcctccgtca cgtgattggg gaggcgaatc
    25741 aacttggcgc tcaaccacac tccgccggcc gggtctctct catatacccc cactaactcg
    25801 gcctcggctt gtgtgacagc gtcatttttc gtctgcatcg cacctgggct tacctcaaga
    25861 atccgcaacg ttccgaatcc tgataacgcc gaagccagcc ccgattcgaa ccgaccacta
    25921 atcgcgaaac gccggctgga cctggcctcg acgaactctc gaacaaccgt gagcactttc
    25981 tccgaggtat tcaccttctg agcgagcttg tgggcgagag cgtacaatgc cttccccagg
    26041 agactcgagt cgccggacgt ggcaggagga atgagcgtaa atctgatcgc ctgcgcatca
    26101 aagtgtcggg cgagttgatc ccggagagag gacgtcaggt ccagcgcaag ctggtcgagt
    26161 gccaccctgg ctaggaccgt aacggagtgt tcactaggct ccaccttccg caccgacgca
    26221 tcgacatgcc cggcggcatc ggtgcgcagc agccgatcga gttcaccctg tcccttctca
    26281 aatgaaagct ggagggggag ctccgccaca ccctgttcct tgccgcccag gatgccgacg
    26341 atttgcatcc gaagtggttg tgccagtggt tgccccggaa aggcccgttg ttgatcccca
    26401 ctgatctttt tgacttgcaa ggacccgagc acagtctgga tctcctggtg cgtcgccccg
    26461 gcgtcccctg gtccggctaa tccttttcgc tgttctgaat cagtcccatt gaggtgatca
    26521 gcgactttcg cccaggattc ttgggcctcc cgcaattggg ctaatgacag cagaacacgc
    26581 agcacatcac cctgcgcttc atgctgcgca gcgaccttct tagcacgaac cgcctcgtcg
    26641 gtcagttccc ggagtctcgc gagatggtga tagaccgggg aacgcatggt aaaccgagcc
    26701 atcagttgat cctgccgtgc aagcacccgc tttcggaggg cttccgggta gtcggcaagc
    26761 gcctcctcca agctgactcg aacgatgatc gtcgcctccc gatcctctgc ctggatatga
    26821 tgtaccgtcg ctcgagccag cccctggctg tccgtcacgc tgtgtgcatc cacctcagcc
    26881 ttcccggcct ctatgcgaaa ctgaatcggc aggttctgga ttgcgtggtc gccggccatc
    26941 gcccgcacaa caagcggcgc gcccaattgc ggagcgatga cagcccgctg ttcgtcgccc
    27001 gcagctttta ccagtttgag cgatgccaga agtgattcaa actgccgtcg agcttgttcc
    27061 accagcacgg cacgcgattc tcccttggcc tgctgtgcgg caaggtagcg gagacgccgg
    27121 tcgagctgtc tggcagtcgc atccaggtcg agcaattggt accacacacc caacgctcgt
    27181 aacgcctcgc cctgctcctg caaagaagtg gcgtgccggc tcaactctcc tagacgagcc
    27241 tgaaggttct ccagttccac gccccaggcg tttacagcac gatctcgatc caatcgagcc
    27301 aaagcaaaga agtgtgctcc acagggatca gcgtaccgat cgacaatatc gagtccggag
    27361 atcgcgatct gaaccgtctc catcacctcc gacgacgcgc tgtatgaaaa cccctcggcg
    27421 ctggtctcct gttgactgct gcggtccacc gcatcgattt tcacctcaag gctcttcacc
    27481 acctcgagtc gtgcatgccg atcagcctcc tcctgactga cagtggattg ccccatcgcc
    27541 tgccacggag ccgtttgccg tacccaggcg ggagggactg aggattcgca gcgcacggtc
    27601 ttctgcgaag catggtttga gggaagagga ggcttaggtt cgttaagagg cggggttcgc
    27661 ttaggaagcg gcgcaggctt gccggtgcgc aactcctcct gtgccgcttc catgtcggcc
    27721 ttaatggccg cttggtcctc atgggacagc gcgctcgtgc ttggcagagg caacggatct
    27781 ttcgcccctg agtttggagc cgtcgtacat cccgtaacca ggaaaagccc tgtccccgca
    27841 agcatggcga aaccaacgat ggtgcctcga caattgccca tacgccccct ccgtctcacc
    27901 gtctacacaa aggattcagc aacgcttgtc gccggataca ccctggcttg gacttacctg
    27961 atccgaccct ctccatcatt ttcagggtca tcggcccttg gccgccttca ctcgatgacc
    28021 cagtgcccga gactcccaac cggaacccta cctcaccaac gccacgggga aattgctgta
    28081 cccctggggc gtatctgtga tgggatgctg ggcgccgttg gtcagctgcg gcacggtttc
    28141 cgacacatac gtgtcgagct ctttcatcga aaccttccca tctgggaca
//
LOCUS       DAORQN010000008        27037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_122_length_27037_cov_30.411867, whole genome shotgun sequence.
ACCESSION   DAORQN010000008 DAORQN010000000
VERSION     DAORQN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27037
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_27037_cov_30.411867"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..817)
                     /locus_tag="PK614_01330"
     CDS             complement(86..817)
                     /locus_tag="PK614_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80885.1"
                     /translation="MQWDILATILLTATIQSLFGVGVLLFGTPILLVLGYDFITALTI
                     LLPISLTINLLQVSKDYRHVQVPFYRQVLTLSIPCVVLCLLLVTTLKLNIGSIVGLFL
                     IVAALKSIYVPFNRLIESLVRYERTYFIVMGVVHGLTNLGGSLLTAIIHSKQYDKDQT
                     RATTAVSYGTFALFQLVTLGISLGPSAIATSDTALYMAVGVSMFLATELLLYSKMSTE
                     RYRTTFAAFLFLSGTALIFKSLFLR"
     gene            complement(941..>1259)
                     /locus_tag="PK614_01335"
     CDS             complement(941..>1259)
                     /locus_tag="PK614_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80886.1"
                     /translation="AGSRPVFIVFELHQHYQSFQVFGRCYPEEVTGLDGRTVVAEDAM
                     YIALEDQTGYLKLFPTESGWKPGRYKVEIHAGEQINDMSLMGTMRFTITASSDSGAPA
                     RSR"
     assembly_gap    1260..1285
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1286..1554)
                     /locus_tag="PK614_01340"
     CDS             complement(<1286..1554)
                     /locus_tag="PK614_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80887.1"
                     /translation="MTGRLGGVYQDGMRQLLWFCCPIFLLSGSLLWAEDLPLTRNVPI
                     PDFQNRTESAKPYNFDAPPEGMFRHIVLAEGFDEELGFQRTHEIVP"
     gene            complement(1710..1961)
                     /locus_tag="PK614_01345"
     CDS             complement(1710..1961)
                     /locus_tag="PK614_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80888.1"
                     /translation="MDGVAVGPFDVTIGGDFLVAFGDLLEVVGGQRMCQSIIGRGECL
                     IEAALLIAPQLIKQGVDMGLPIIDRLILPKRLRQRVGIL"
     gene            1993..3168
                     /locus_tag="PK614_01350"
     CDS             1993..3168
                     /locus_tag="PK614_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80889.1"
                     /translation="MEWGPGNGNLAACFLSQLQRLDKDGQVYPRVRYVLVDSQAHALE
                     RARAHPDLAPHLAKVESLCAEVENLATIADGTVDRILCNELWNELTTKLMVKKGGEFE
                     EEHLRPNLNERKAAAIADWSGFVRAFEAKDIERLRQFPPFLDDLIWEREYHKVDWKDV
                     PYRKTITEFMKAIDDEVLVPVNLGACASLKEAKRVLAQDAVGFSSFDAGTADMEVLND
                     PDKPCYGQFGGQYSFMVNLALIQAVAKHLGLNAVTIETQREFVGSRLGTNVMTLMDLL
                     ACHPMVGSKVQPWELDRLTVKTIRTLNETYESPYQRKIEFPLRSEMPVEERDAAQGIL
                     LSLKPNGIPDTIAYVTEEELSRAQPELEHLGYEREAVLMALGAPPSPVEYYHFACRP"
     gene            3441..3725
                     /gene="tatA"
                     /locus_tag="PK614_01355"
     CDS             3441..3725
                     /gene="tatA"
                     /locus_tag="PK614_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB80890.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     HEADAIDVTATEAESAPAQPSAQIQQTGQPATPPPAQQAGAAPPPPTTQG"
     gene            3820..4119
                     /locus_tag="PK614_01360"
     CDS             3820..4119
                     /locus_tag="PK614_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB80891.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEIGRQVGKAVKGFKETAD
                     DLKKTVEPEINMIQQEMKMVEQDFESSMKEAEEQINHATSGVEHGAEESGLPKQA"
     gene            4371..6083
                     /locus_tag="PK614_01365"
     CDS             4371..6083
                     /locus_tag="PK614_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRB80892.1"
                     /translation="MKRIHGRTRWGRLAAIFGAVAVTALQCLAVPAFAGYELPPGERI
                     TNLPAIPRNMPQKEAYELYDPVIGRNFDVKNLWLRADLRVRPEMRNNTCFGGGMGVGG
                     ACNTFGGANARGVNPTSGNKGNDFFVQQWVRLGIGYDLSPDVNFYMEIIDSATWGANG
                     NPANAGNGGDPLTHNGGVSGGGGNNGRLGVRAAYMLVRNLGGIQGLNMKAGRQYVIFG
                     NHSLFGHFDWANTGYSHDGVMFAYQTKSWDSYFGWFRNSETDIGQAAAVGSGGANVAG
                     GTTNGTFTGVGGTGNTVAGDANRDADMFIFYNQFKMIPGMVIEPFYVYYKNNLSSADN
                     AGQGLGTAKHSNQTRHMIGNRIELRKGGFDFSNEIAWQFGQMGQVGACQGEQKCLHIN
                     AWATRNWIGYTFYDTSWKPRLAFNFDYASGDSRNNTCGATPGSCSTANTFENFFPTNH
                     IHMGYMDVQAWKNMLSPSVNFQARPSARDHIELWYTNLNLANSKDCWYRGAQGCYVFS
                     NPNNTKTHIGDEIDVAYTRMFADGKVALQAAYGTIFGGGYLTSTLNQTANQHWAYMSL
                     WMNF"
     gene            6168..6596
                     /locus_tag="PK614_01370"
     CDS             6168..6596
                     /locus_tag="PK614_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80893.1"
                     /translation="MKRLITVALGVAALTVGSMPVTAQAQVADAIQAVNDAIVELTDA
                     PGLGRRATVDVTKRWGSDVTVIDSATAKLQDGLSKAQSGGVGADATRQLKLAVEYGKA
                     RLHKEARLSAQGALRYLCVANQDQGPGCDTAPKFGSYTAP"
     gene            6700..8676
                     /locus_tag="PK614_01375"
     CDS             6700..8676
                     /locus_tag="PK614_01375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRB80894.1"
                     /translation="MAAATACGERAGLPAVSRSPYTRLTRHLHHRSCVSSLTVDDLST
                     QLSRQFGFSAFRPGQREVIDAVLSRRDAMAVMPTGQGKSLCYQLPATLLPGVTLVISP
                     LIALMQDQVNSLKARGIAAAAFHSGLSEQERDRVVLDLKLRRVQLLYLAPERMQHERF
                     LRLLRSLWVSLLVVDEAHCISQWGHDFRPDYLNIGRLRRELENPPCLALTATATARVQ
                     ADLCDRLSLQDPLRLVTGFRRSNLALSVRLCRSRQEKLATLDRVVREYETGTILIYCA
                     TRRAVEEVAACLGQSQSSVGYYHAGLSDEERRKVHDEFRVGTVRILAATNAFGMGIDK
                     SDVRLVVHFDIPGSLEAYYQEVGRAGRDGRPAACLLLFHERDVATQEYFIQQASKESG
                     SSARADRMRTLLQDLLDYVSGSTCRQLAILDYFSDEAERALGPCGLCDRCVVTPQPSA
                     HVVGDEATCAKAVLAAVSWCMGRFGVTRIVDMLRGSRSKALLTYGAEECPGYGGYHAW
                     SKTALTRLVKALIEAGYLQVEGLEYPTLDLTRKGQEALKGIAPLVLCEQAEAPAASAL
                     KPQGSGRDHAMAPLHTTVVDTQLFERLRQLRRELAEEEGVAPFVIFHDKTLRNIAGYK
                     PVTLAALLEIPGIGEVKVERYGRRVLGVVNEACD"
     gene            complement(8755..9375)
                     /gene="thrH"
                     /locus_tag="PK614_01380"
     CDS             complement(8755..9375)
                     /gene="thrH"
                     /locus_tag="PK614_01380"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HRB80895.1"
                     /translation="MQNPVIVCLDLEGVLVPEIWVNFAIKTGIEELKITTREMPDYDA
                     LMTRRLSILDQHGLTLADIQSVIDAMGPMEGAAEFIAWLRERTQVIILSDTFYEFALP
                     LMRQLGYPTIFCNQLEIGPTGRIVHYKLRQPNQKKHAVAALKNLNFRVMAAGDAYNDT
                     AMLGEAHAGFFFRPPDHLPKEFPQFPVTNTYAELQEQFRKAGNLRE"
     gene            complement(9459..10256)
                     /locus_tag="PK614_01385"
     CDS             complement(9459..10256)
                     /locus_tag="PK614_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB80896.1"
                     /translation="MSNHTAVEHFEISLQTPSGEISTAVGVPTSFVPITAILPLMRSL
                     GEEAQVLEQRRLLQTGQRVSCEKGCAACCRMLVPISVPEAFALTNTIDRLDQNERNRL
                     LSKLDLAQQQLARAGILKQLSSLADSSEPLSDEAIEPLNRAYYALRMPCPFLDNETCS
                     IYADRPAACRELAVTSPATECQDMTTQTVQPVPVAVRLSTALSLLWADLTGTAPRLIP
                     LPLAVDWARRHKEEQTNQWAGTELFEKAMDKIWRYLSQEKARRSNAG"
     gene            10459..10788
                     /locus_tag="PK614_01390"
     CDS             10459..10788
                     /locus_tag="PK614_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80897.1"
                     /translation="MRDLVEQARQESQAQEAFGFSAPPYQPDHAIADLLAILDDRIES
                     EGVQVGMPLEFLHEMWRRCNEARNEIAERVWLERSLDAPDVSKARIRELTYASVIEYL
                     ENESLGD"
     gene            10791..12005
                     /locus_tag="PK614_01395"
     CDS             10791..12005
                     /locus_tag="PK614_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRB80898.1"
                     /translation="MRGTGLRWRRLALLMLVVMTAILSDETKARGQTDTPANRPSLTL
                     SPISTQGLRQPLYLTHAGDGSGRLFVVEQPGRIRILTEDRLLAVPFLDIQDLVLAGGE
                     RGLLGLAFHPDYAHNGRLFVSYTRQPDGATVVAEYRRGSREDQADRQERVVMIVQQPF
                     ANHNGGMIAFGPDGALYIGRGDGGGRGDPDSRAQNPQDLLGKILRLDVNQGEPYAIPA
                     DNPYASGGGRREIYAIGLRNPWRFSFDRETGSLWVADVGQDAWEEVDLVVRGGNYGWR
                     VMEGRHCFLPKIDCDKPGLQLPVLEYGHEAGRCSITGGYVYRGSSQPALKGLYVYGDY
                     CSGDIFAARLEGGAHPRVIEGPEILLRSGLRISSFGEDEAGELYVVDHGGGVYRLESS
                     RESRPYSFPAIR"
     gene            complement(11981..12793)
                     /locus_tag="PK614_01400"
     CDS             complement(11981..12793)
                     /locus_tag="PK614_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRB80899.1"
                     /translation="MDLTPIWFGVYKALKYLLYPLSWILIAGLLALLTACLPVSPGRR
                     TWVRRCSFLTVLLLFLTATPILSSMYIALLEAQYPPFQATSTSKFDAVVVLAGGVFQK
                     GSLRPTDEVSDASRQRTGCGAGLWQQNLAPKLLLTGGDATVFRTGLMESHEMKRWALR
                     LGVPESAILIEEKSRTTYENAVQTKAMLGSGRILLVTAAYHLPRAVRLFEKQGFVVTP
                     FPCGYESQHTPQQAWEQATAFDFIPTARSLLITTQAIDEVFGILVYRIAGKL"
     gene            12875..13360
                     /locus_tag="PK614_01405"
     CDS             12875..13360
                     /locus_tag="PK614_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRB80900.1"
                     /translation="MADGRHYWLMKSEPEVFSIDDLAGSPKSTTSWDGVRNYQARNFM
                     RAMKVGDQVLFYHSNANPPAVVGVAEVVRAAYPDATQFDKRDSHYDPASNPAQPRWDM
                     VDIRFVRKFLAPLSLDLLRAKAGLKGMELLRKGSRLSVQPVRAAEWNEIIRLEEVQAD
                     S"
     gene            complement(13416..13703)
                     /locus_tag="PK614_01410"
     CDS             complement(13416..13703)
                     /locus_tag="PK614_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRB80901.1"
                     /translation="MAEATYFDTTVRGMVRIPNKVILPFGYHIMIRQVTDSEMDRQDS
                     NADGIWDNEAKTIYIRKRLPVTRRRYILAHELGHAWLDWQHRYLDDGKARS"
     gene            13869..14642
                     /gene="ricT"
                     /locus_tag="PK614_01415"
     CDS             13869..14642
                     /gene="ricT"
                     /locus_tag="PK614_01415"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRB80902.1"
                     /translation="MKDWMVYHAPMVEKLLAQELANPYPAVVRIVGVKIRDRGEVKKF
                     DAGEASLVMGDRVLLEVAGELSYGVVYGAPQVMPFIPPMRVLQPITRKATTEDVATID
                     RYERLASEGMKACREQAAALGLRMKLVEVFCSFHRRQMTFVYTAEDRIDFRELVRLLA
                     RRFGGRIEMRQVGVRDEASRLGGIDTCGLVLCCAAFLTEVKPVTVKQARVLGLPVDDP
                     KLLGVCGRLKCCLLFEAMDNPSSKSSALINPSRSRLASS"
     gene            14766..15857
                     /locus_tag="PK614_01420"
     CDS             14766..15857
                     /locus_tag="PK614_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRB80903.1"
                     /translation="MPQVLVLGAGKIGSLVAGLLSATGQYQVHLADLTLDAPKRLVDE
                     LSLSAVTPCALDVRRPDLVAGYVGSHRFDAVISSLPYFCNPTVAEIARAHQLHYFDLT
                     EDVAVTGRVKAISEGADRAFIPQCGLAPGFISIVTNDLIGHFESIDNVKMRVGALPVH
                     PSNALKYSLTWSTDGLINEYANVCVGIEGGEEVHLQPLEGYETIELDGLLYEAFNTSG
                     GLGTLADTYRGRVRTMNYKTLRYPGHCEKIQFLMKDLKLNEDRETLKRILERAIPQTQ
                     QDVVLIYAAVTGTRQGELFEETYVKKVYPRTILGRLWSAIQVTTASSLCCVVDLVMAR
                     PSAYRGFVTQEHFHLQDVLNNRFGDCFRA"
     gene            15865..17421
                     /locus_tag="PK614_01425"
     CDS             15865..17421
                     /locus_tag="PK614_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB80904.1"
                     /translation="MTIQELFKTLGLTQEQPGGCLSSSTWSRTTDAGIVESRNPSTGE
                     PLAQVYGCSLADYDALVAGSQQAQASWRMVPAPKRGEVVRLIGQALRVHKDALGTLVS
                     LEVGKIKAEGDGEVQEMIDMADFAVGLSRMLYGQTMHSERAQHRMYEQWHPLGVVGVI
                     TAFNFPVAVWAWNAFIAAVTGSTVLWKPSPKAPLCALAVQRLCNRVLEEAGYPGVFAL
                     FTTDRVELAERMVRDERLPLISFTGSVPVGRHVAEVVGHRLGRSLLELSGNNAVIVDE
                     TADLDLATRAIVFGAVGTAGQRCTSTRRLIVHESQYELLVPRLLSAYAQVKIGDPLEP
                     DMLMGPLIDGEAVTRYLSALEEVKKAGGEVLCGGCVLSRPGYFVEPTIVRAQNHWDIV
                     QRETFAPILYVMTYRTLDEAIRMHNAVPQGLSSALFTTHLRNSETFLSAIGSDCGIAN
                     VNIGTSGAEIGGAFGGEKTTGGGREAGSDAWKAYMRRQTNTINWGTELPLAQGVTFGR
                     AEGGNDGARS"
     gene            17402..18004
                     /locus_tag="PK614_01430"
     CDS             17402..18004
                     /locus_tag="PK614_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80905.1"
                     /translation="MMGQEADLDAVMARMTADFLAQNSAGRMLRRALDDVGVGFIPVI
                     DHVTIRTMDIDRRAEEFLALGYRYDETLHYDDWYAKVFRWAGYPALFVDQAYPDDRGK
                     TSIIPRWVEKFGDQVFHHVAVRVEDIEQAILKLKAKGVVFAGEIVGARGGTLRQIFTA
                     PEMLDGQPFSVLELAERHQGYQGFLPPQADSLMRSTMRGL"
     gene            18175..19245
                     /locus_tag="PK614_01435"
     CDS             18175..19245
                     /locus_tag="PK614_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB80906.1"
                     /translation="MVQSNATLRLILALLVLCALGAGSGSVQASPSGHAPHGTVTIDG
                     VTVPDVGPLPTVVPIPSSNLNYTAKIELGKQLYFDGRLSKNNAISCAFCHNPGTGFAD
                     PRQTSIGVGGGVGGRQSPTVYNTGLNHVQFWDGRARSLEEQAIGPIHNPVEMAETHEH
                     VVAKLGKIKGYQQQFRAVFGTDVNLQGIAEAIAAYERTVLSTNSAFDKYVLGAQKAMD
                     EAAVRGLALFKGQARCILCHNGPNFTDNQFHNLGVPQVGPMKEDLGRFAVSRAEKDRG
                     AFKTPTLRSITETAPYMHDGAFKTLEEVVEFMDQGGGSNPNLSPLVKPLNLTAEEKSD
                     LVAFLKALAGEPIPFSMPKLPK"
     gene            19359..19865
                     /locus_tag="PK614_01440"
     CDS             19359..19865
                     /locus_tag="PK614_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HRB80907.1"
                     /translation="MFRNLCVLGASTIALALGGPALAADMKPGPADGFDIHVMAPHKM
                     EDGSVAGPFHHYCKAVKPEVLQCLLFESTDPNAVLTDVEYFVAKPVSRSAVPLDVWNK
                     FYHDHEVEIATGRVQVLDMPDAQAKEVAAAAAKTDGIIFHLWPKGAKAPDGSVEHPTS
                     VGHKPRTE"
     gene            19918..20472
                     /locus_tag="PK614_01445"
     CDS             19918..20472
                     /locus_tag="PK614_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HRB80908.1"
                     /translation="MRSAILMFGLCAAALTTAGCAETAREMKSSSAAPAAAMAAKAMP
                     TPAQGYTIHVMAPHKFEDGSVHGPYHHYCKPISPEVLQCLLFESTDANALLTDIEYFV
                     AKSVSRAHVPLETWNKYYHDHEVEIATGRVQILDMPEAQAKEVAAIAAKTDGIIFHLW
                     PDGAKAPNGQVGHPQSVGHKHRTE"
     gene            20554..21000
                     /locus_tag="PK614_01450"
     CDS             20554..21000
                     /locus_tag="PK614_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB80909.1"
                     /translation="MDAGRLCLMGWLLFGGVSVGWAANSEVLRPRVPIDQIEAARTVT
                     NPFPVTPETLQQGKALFEGKAFCRACHGSDGKGLGMDLDYSTFKGPLPRNFTDRLWQQ
                     ARTDGELFWILKNGSPGTDMAPFIPLVLTEEEAWQVLMYVRSFGGR"
     gene            complement(21706..22080)
                     /locus_tag="PK614_01455"
     CDS             complement(21706..22080)
                     /locus_tag="PK614_01455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB80910.1"
                     /translation="MSHYSPASLGPLDRPPSSEAILIFQRSRDRFGIVCPVMFSGALF
                     IGEGLMHNLSQTGCLMECDRRMLEGSYMAVRLLLPDTTPALIIELAAVRWIREEYFGI
                     EFLKLPTSDQARLAHFLLAHQR"
     gene            24476..24598
                     /locus_tag="PK614_01460"
     CDS             24476..24598
                     /locus_tag="PK614_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB80911.1"
                     /translation="MALVASCARQAKIEGFHFHDVRHTFATILLQSGLDLYKVQ"
     gene            25204..26544
                     /locus_tag="PK614_01465"
     CDS             25204..26544
                     /locus_tag="PK614_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB80912.1"
                     /translation="MHHIVEDGAECEHVAGCSSCRNGETVPFECSAKLRGEIERRAAF
                     YGRTWDEELNHILAIAYEQREPDVDDEDMTRRLNELRRRGELPSQRRAKVEIRLASID
                     RLPDGVDVPRPTTVKELCDIYLEYKLKGRRSYANVRRTVRHTMEQTGFADVKLSDLTS
                     GVIKRWHAGMASRHVTGNNGLKTLRSAYRFCRSLELLDSDPTAGIKRFREDARERYVT
                     PSEMPRLMHALEGAPLRAQTFFLTILFTASRPGEVAGMQWKDLDFVGMRWRKPTSKTG
                     RSQEVVLNPIVYRLLRQLPQVSDYVFPGKDLSKPWNESTQRKAWDRVRSVAKLPDVIA
                     YDLRRTMASWMAQHGLNLSVIQQSLNHSTLQHTSRYARLDVQTAGRALDGVSKHMAAS
                     NRFFNDLCAPLTSEELSPEAQEMGTTAEQVQARHTETSSADESGTEPEDMEIPG"
     gene            26637..>27037
                     /locus_tag="PK614_01470"
     CDS             26637..>27037
                     /locus_tag="PK614_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80913.1"
                     /translation="MKGLGAVLLGSLLLLQGCAGLILREHDSVGTKAAKVAARVLAWP
                     VTLTLSEVVIGSIKQREELEASQQQAQLSQRNHFDGLVGRLTYSEALVRWGPPAQSQA
                     HGGMIVAVWQSRRASPGYLIMPPIPGNPYSSS"
BASE COUNT         5621 a         7097 c         8426 g         5867 t
ORIGIN      
        1 cgaaggggcg tagggcgtac ccttgtggta cgttgagccg ctgagcgacg cgagaacgcc
       61 gctggcggag tttttccgca tccggttagc ggaggaagag ggacttgaag atcagggccg
      121 tgccggacaa aaagagaaat gcggcgaagg tggtgcggta tcgttcggtg ctcatcttgc
      181 tatagagcag cagttcggta gccagaaaca tgctcacgcc cactgccatg taaagtgcgg
      241 tgtcggaggt ggcaattgca gaaggaccga gggagatgcc caacgtgacg agttgaaaca
      301 gcgcgaacgt gccgtaggac acggcggtcg tggcgcgggt ctgatctttg tcgtactgtt
      361 tgctgtggat gatggccgtc aacagcgagc cacccagatt ggtcaggcca tgaacaacgc
      421 ccatcacgat gaaataggtg cgctcatatc gcaccagcga ctcgatcaac cgattgaacg
      481 gcacatagat gctcttgagc gccgcgacga tcaggaagag cccgacaatg ctgccgatgt
      541 tcagcttgag tgttgtgacc agcaggagac acagcaccac gcagggaatc gacagggtga
      601 ggacctgccg gtagaagggc acctggacgt gtcggtagtc cttgctgact tgtagcaggt
      661 tgatggtcag cgagatcggg aggaggatag tcagggcggt gatgaagtca tagccgagca
      721 cgaggaggat cggagtcccg aacaacagca ctcccacgcc gaagagcgac tggatggtgg
      781 ccgtcaggag gatggtcgca agaatgtccc actgcatggc gatgatgctc ctctcggacc
      841 gaacgtgtcc aagatagaca ggctctcaac tcccggtccg gtagcccggt atatcccagc
      901 tttggcgcgg gcgtcaatca gcccacaggc ggggcgtggt ttaccggctc ctggcagggg
      961 cccccgagtc tgaggaggcg gtgatggtga agcgcatggt gcccatcagg ctcatgtcgt
     1021 tgatctgttc gccggcatgg atttccacct tataccgtcc tggtttccag ccgctttccg
     1081 tcgggaacag cttcagatag ccggtttggt cctctaaggc gatatacatc gcatcttcag
     1141 cgaccacggt tcttccgtcc agccctgtca cttcctccgg gtagcagcgc ccgaatactt
     1201 ggaacgattg gtaatgctga tggagttcga agacgatgaa gaccggtctg gagccggcgn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnggaac aatttcatgg gttcgttgaa atccgagctc
     1321 ctcatcaaac ccttcggcga gcacgatgtg gcggaacatc ccttcgggtg gggcgtcgaa
     1381 gttatagggt ttcgcgctct cggtgcggtt ctggaaatcg ggaatcggca cattgcgggt
     1441 gagcgggaga tcttcggccc agagaaggga gccggagagc aagaagatgg gacagcagaa
     1501 ccagagcaac tgcctcatcc cgtcttggta cactccgccc agccggcctg tcaaggaaag
     1561 gctgcgcgcc gggtccttag ccggacggtc cgcttggcaa agcgggggcc acgaatcgtg
     1621 cggggcaggt tgcgggtgaa aattgcttcc tgttagactt tcctcctttc tacgggggga
     1681 cagacatgga acagcacctc atgacgcagt tagaggatcc cgacgcgctg ccgcagccgc
     1741 ttggggagta taagccggtc gattattggc aggcccatat caacaccctg ttttatcaac
     1801 tgcggggcga tcagcaacgc agcttctatc agacattcac ctcggccgat tatcgattgg
     1861 cacatgcgct ggccgccgac tacttcgagc aggtcaccaa acgcgacaag aaagtcgccg
     1921 ccaatcgtga cgtcaaacgg cccgactgcg acgccgtcca ccgacgccac tcctcaagca
     1981 cagttgacgg tgatggagtg gggccccggc aacggcaatc tggcggcctg cttcctcagt
     2041 cagctgcagc gtctcgacaa ggacgggcag gtatacccgc gagtgcggta tgtcttggtc
     2101 gattcccagg cccatgcgtt agagcgggcg cgtgctcatc cggatctggc tccccatttg
     2161 gcgaaggtgg agtcgttgtg cgccgaggtc gagaatttgg cgactatcgc cgacggcacc
     2221 gtcgatcgca ttctgtgcaa cgaactctgg aacgagttga cgaccaagct catggttaag
     2281 aaggggggcg agttcgaaga ggagcacctc cggccgaacc tcaacgagcg aaaggccgcc
     2341 gccattgccg attggtcggg atttgtccgt gcctttgagg cgaaggacat cgaacgcctg
     2401 aggcagtttc cgccctttct ggacgacctg atctgggagc gcgaatacca caaggtggac
     2461 tggaaagacg ttccctatcg caagacgatc accgagttca tgaaggcgat cgacgatgag
     2521 gtgttggtgc cggtcaatct cggcgcctgt gcttccctga aggaagcgaa gcgggtgttg
     2581 gcccaagatg ccgtgggatt cagcagcttc gatgccggga cggctgacat ggaagtcctc
     2641 aatgatccgg acaagccctg ctatggccag ttcggcggcc agtacagctt catggtgaat
     2701 ctggccctga ttcaggctgt ggccaagcat ctggggctga acgcggtgac gatcgaaacg
     2761 cagcgggagt tcgtcgggag ccggctgggg acgaatgtga tgacgctgat ggatttgctg
     2821 gcctgccacc cgatggtcgg ctcaaaggtt cagccctggg agctggatcg cctcacagtc
     2881 aaaaccatcc gcacgctgaa cgagacctat gagagtccct atcaacgcaa gatcgagttc
     2941 ccgttgcgca gtgagatgcc ggtcgaggag cgcgacgcag cgcagggtat tctgctctcg
     3001 ctcaaaccga acgggattcc cgataccatc gcgtatgtca ccgaagaaga attgagccgg
     3061 gcacaaccgg agttggagca tctgggctat gaacgcgagg ccgtgctgat ggcgctcggg
     3121 gcgcctccga gtcctgtcga gtactatcac tttgcatgtc ggccgtaagc cgcgccgggt
     3181 cgtgcggcag gacaggtcca ccaggtgatg tttgcaggag aaagagcttg actaattaat
     3241 tccatttcta tagcctttcc cgcgatttcg aagagaagat ccgtctctcc tccgtgcatc
     3301 gcgagcattc aaacgggatg ggatctcgca ggcaagatca tctcagacga cgttcttcac
     3361 gagagggctt tgcggtcgtg agcgcgtgaa tatacgtcgt gcgaataatc gcacagctgt
     3421 aacaaggagc atacagtacc atgtttggtt cgtttggctg gatggagctg ttgctgattc
     3481 tcatcatcgt cctgatcatc ttcggtgcgg ggaaaattcc ccagctcggt gagggattag
     3541 gtaaagcgat caaggggttc aagaaatcgg ttcacgaagc ggatgccatt gacgtgacgg
     3601 ccaccgaggc ggaatctgcc ccggctcagc cgtcggcaca gatccagcaa accggacagc
     3661 cggccacacc gccgcctgcg cagcaggccg gtgcggctcc accgccaccg acgacgcagg
     3721 ggtaacttgc gaaactgagg atgcagaccg aatgacggcg atgtgacctc ggagtcccgc
     3781 cgctcggtct tcagtacacc cccctcagta tggaacacca tgttcggtct tggcgctggt
     3841 gaaattctca taatcctggt cattgcgttc ttgctcttcg gtcccaagca gttgcctgag
     3901 atcgggcgtc aggtgggcaa ggccgtgaaa ggatttaaag aaacggcgga cgatctgaag
     3961 aagaccgttg agccggagat caacatgatt cagcaggaaa tgaaaatggt ggagcaggat
     4021 ttcgagtcgt cgatgaagga agcggaagaa cagattaacc acgcaacatc gggcgtagag
     4081 cacggggcag aggaatcggg tttgcccaag caggcctaat accattcata tccgctctac
     4141 ggctgtgaag ggaggtgacc acagcaacaa tacaagacct gacggtgtga ggagctcgtt
     4201 tcaggattga agcgaggatc ggggccgaat ggtgggcacc attcaggttc gagcgtcccg
     4261 acatcacaca cacagtgggc tttgtaatgc aatagcggtt ggatgtatag gcggcactcg
     4321 gagtgagtgg gtacacaacg ggttgccatc caattcacag gaggaacgct atgaaacgca
     4381 tccatggacg gaccagatgg ggccggttgg ccgccatctt cggcgctgtg gcagtgacgg
     4441 ctctgcagtg tctggcagtc ccggcctttg ccggatatga acttcccccg ggcgaacgta
     4501 tcaccaacct tcccgccatt cctcgcaaca tgccgcagaa agaagcctac gagctgtacg
     4561 atccggtcat cggccggaat ttcgacgtga agaatctctg gttgcgcgcc gacctgcgcg
     4621 tgcggcctga aatgcggaac aacacctgtt tcggcggtgg catgggtgtc ggtggtgcct
     4681 gtaatacctt cggcggcgcc aacgcccgag gagtcaaccc gacatcaggg aacaaaggca
     4741 acgatttctt cgtgcaacag tgggtgcggt tgggcatcgg gtacgatctc tcgccggatg
     4801 tcaatttcta catggaaatc atcgacagcg cgacgtgggg ggccaatggc aaccccgcca
     4861 acgccggcaa cggcggcgat ccgttgaccc ataacggcgg ggtctcaggc ggcggtggaa
     4921 acaacggtcg tttaggtgtc cgtgccgctt acatgttggt gcggaacctg ggtggaatcc
     4981 agggactcaa catgaaggcc ggtcggcaat atgtcatttt cggcaaccac tccctgttcg
     5041 gccacttcga ttgggccaac accggctatt cccatgacgg tgtgatgttt gcctatcaga
     5101 ctaagagctg ggacagctac ttcggctggt ttcggaattc agaaactgac atagggcaag
     5161 cagcggctgt aggtagtggt ggcgccaatg tcgctggcgg cactactaac ggaactttta
     5221 cgggcgttgg cggtacaggc aatacagtgg ccggagatgc caaccgcgat gccgacatgt
     5281 tcattttcta caaccagttt aaaatgattc ccggcatggt cattgagccg ttctatgtct
     5341 attataagaa taacttgagt tcagcagaca acgctggaca ggggcttggc acggccaagc
     5401 actccaacca gactcgtcat atgatcggca accggatcga actgcgcaag ggcgggttcg
     5461 atttcagcaa cgaaattgcc tggcagttcg ggcagatggg ccaggttggt gcttgtcaag
     5521 gcgagcagaa atgtttacac atcaatgcct gggcgaccag aaactggatc ggctacacct
     5581 tctacgacac atcgtggaag ccgcggttgg cgttcaattt tgactatgcc tccggcgaca
     5641 gccggaacaa tacctgcggt gcgacgcccg gttcttgtag tacagccaat acgttcgaaa
     5701 acttcttccc gaccaaccac atccacatgg gttacatgga cgtgcaagcc tggaagaaca
     5761 tgctgtcgcc atccgtcaac ttccaggccc gtccttcggc gcgtgaccat atcgaattat
     5821 ggtacacgaa tctgaacctc gccaattcca aggactgttg gtatcgaggc gcgcagggct
     5881 gttacgtctt ctcgaacccg aacaatacca agacgcatat cggcgacgaa atcgacgtcg
     5941 cctacaccag aatgtttgcg gacggcaagg ttgcccttca ggctgcctat ggcacgattt
     6001 tcggcggtgg ctatttgacc agcacgttga accagacggc caaccagcat tgggcgtaca
     6061 tgtcactgtg gatgaacttc tagggagaag ccgttagctc atagctgtca gccatcagcc
     6121 gctgaatgct gtgggctgat cgctacagca agacaaagga gatcgcgatg aaaagactga
     6181 taacggtagc gctgggcgtc gcagcgttga cggtcggaag catgccggtc accgcgcagg
     6241 ctcaggtcgc ggatgcgatt caggcggtga acgatgccat tgtcgagttg accgatgcgc
     6301 cgggtctggg caggcgggcc accgtcgatg tcaccaagcg atggggctct gatgtgacgg
     6361 tgatcgacag cgcgacggca aaattgcagg atgggctcag caaggctcaa tcaggcgggg
     6421 tcggcgccga tgccacgcgg cagttgaagc tggcggtgga atatggcaag gctcggctcc
     6481 acaaggaagc ccgtttgtct gcacagggcg ctctgcgtta cctctgtgtg gcgaaccaag
     6541 atcaaggacc gggctgcgac acggcgccga agttcggcag ttataccgcg ccgtagcagg
     6601 tctgtgaacg acagtgcgag gcctgaggga gatcccctgc ctgtctcgaa gagtgaaacc
     6661 ccgccgggtg atccccggcg gggtttcttt tttcctgcca tggcggcggc gacggcttgt
     6721 ggcgagagag caggcttgcc cgcagtcagt cgatcgcctt atactcggct cactcgtcat
     6781 cttcatcatc gttcgtgcgt ttcttccctc accgtggacg atctgagcac acagcttagc
     6841 cgacagttcg ggttctcggc cttccgaccg ggccaacgcg aggtgatcga tgccgtgctg
     6901 tcacgtcgtg atgcgatggc cgtcatgccg accggacagg gcaagtccct ctgttaccag
     6961 ctcccggcaa cgctgttgcc cggggtgacc ctggtcattt ctccgctcat cgccttgatg
     7021 caggatcaag tcaacagttt gaaggcacgg gggattgccg cggcggcgtt ccactccggg
     7081 ctctcggagc aggaacggga tcgggtggtg ttggatctca agctgcgacg ggtgcagttg
     7141 ctctatctcg ctccagagcg gatgcagcac gagcggtttc tccggctgct ccgctcgctg
     7201 tgggtgtcgt tactcgtcgt cgacgaagcg cattgtattt cccagtgggg gcacgatttc
     7261 aggccagact atctgaacat cggtcgcttg cgccgggaac tcgagaaccc tccttgcctg
     7321 gccttgacgg ccacggcgac cgcgcgcgtg caggcagatt tgtgcgatcg attgtcactc
     7381 caggacccgc ttcgcttggt gaccggcttt cgacgctcta acctcgcgct gtctgtccgc
     7441 ctctgccggt cccgccagga aaagctcgcg acattggatc gtgtggtgcg tgaatacgaa
     7501 acgggcacca ttctgatcta ttgcgccacg cgtcgggcgg tggaggaggt ggcggcctgc
     7561 ctggggcagt cgcagtcgtc cgtcgggtac tatcacgccg gcttgtcgga tgaagagcgg
     7621 cggaaggtgc atgacgagtt tcgggtggga acggtgagga ttttggcggc gaccaatgcg
     7681 tttggcatgg ggatcgataa atcggatgtc cggttggtgg tgcatttcga tattccggga
     7741 agcctggagg cctactatca agaagtgggc cgggcggggc gtgatggccg acctgccgcc
     7801 tgtctgctgc tgtttcatga acgggatgtg gccacccagg aatacttcat tcagcaagcg
     7861 tcgaaagaat ccggcagctc ggcacgcgcc gatcgcatga ggacattgct tcaggacttg
     7921 ctggactatg tgtcggggtc gacgtgccgg caactcgcca ttctcgacta cttcagtgac
     7981 gaggccgaac gggcccttgg gccctgcggg ctgtgtgatc gttgcgtggt gacgccacag
     8041 cccagtgcgc acgttgtcgg tgatgaggcc acctgcgcga aggctgtcct ggcggcggtc
     8101 tcctggtgta tgggccgatt cggtgtgacc cgcatcgtcg atatgctccg cgggagtcga
     8161 tcgaaggcgc tactcaccta tggggccgaa gagtgtccgg ggtatggggg ctatcacgca
     8221 tggtcgaaga cagcgttgac tcggctcgtg aaagccctga tcgaggcggg atatctgcag
     8281 gtggaaggac tggagtatcc cacgctcgac ctgacgcgga agggacagga ggctttgaag
     8341 ggaatcgctc ctctagtatt gtgcgagcag gctgaagctc cggcagcgtc ggcgctgaaa
     8401 cctcaaggga gtgggcgaga tcacgcgatg gcgcctctgc ataccaccgt agtcgacacg
     8461 cagctctttg aacggcttcg tcagctccgg cgtgagctgg ccgaggaaga aggggtcgcg
     8521 ccgtttgtta tttttcatga caagaccctt cggaatatcg ccgggtacaa acccgtgacg
     8581 cttgccgccc tgctggagat ccccgggatc ggcgaggtca aggtggagcg gtatggccgc
     8641 cgagtgctgg gcgtggtgaa tgaggcctgc gactaagccg tcttctcgtt attgtctaac
     8701 gaccccttca atgccatttt gccggagaag aaggatttcg tagccatttg gctattattc
     8761 tctcagattc ccggcctttc gaaactgctc ttgcagttca gcatacgtgt tcgtcaccgg
     8821 aaactgaggg aactctttcg ggagatggtc aggggggcgg aaaaagaatc cggcgtgagc
     8881 ctctcccagc atggcggtgt cgttgtaggc gtctcctgcc gccatgacac gaaaattgag
     8941 attcttcaac gcggccaccg catgtttttt ctggttcggc tggcggagtt tgtagtggac
     9001 gatcctgccg gtcggtccaa tctccagttg attgcagaaa atcgttggat atccgagctg
     9061 ccgcatcaag ggaagcgcga attcataaaa cgtatccgac agaataatga cctgtgtccg
     9121 ctcccgcagc caggcaatga attcggctgc gccttccatc ggccccatgg cgtcgatcac
     9181 ggattgaatg tccgccaggg tcaggccatg ctgatctagg atgctcaggc ggcgtgtcat
     9241 cagggcatcg tagtccggca tctcgcgggt ggtgatcttg agctcttcga tgccggtctt
     9301 gatggcaaag ttcacccaaa tttcaggcac cagcacgccc tcaaggtcca gacacacaat
     9361 cacggggttc tgcattcatc ctcctgcggg aattgatggg tggtgaaaag tgggcttttc
     9421 ggtaactggt agtgataggg acaaaccagc ggtctgccct atcccgcgtt actacgacgg
     9481 gccttttctt gactgagata ccgccagatc ttgtccatgg ccttctcgaa tagttctgtg
     9541 ccggcccatt ggttggtctg ctcctcctta tgccgtctgg cccaatcgac ggccagcggg
     9601 agcgggatca gcctgggtgc tgttccggtc aggtctgccc agagcagact caaagccgta
     9661 ctgaggcgaa cggcgacggg aaccggctgg acggtctgcg tggtcatatc ctgacattct
     9721 gtggccggcg acgtgacagc aagctcccgg caggctgcag gccgatcggc gtaaatgctg
     9781 caggtttcgt tgtcgagaaa ggggcagggc atgcgaagcg cgtagtaggc ccggttcaac
     9841 ggctcgatcg cctcgtcact caacggctca gaagaatcgg ccagcgaaga gagttgtttc
     9901 aggataccgg cgcgagccag ttgttgctgg gccaagtcga gtttggacag caggcgattc
     9961 cgctcgttct ggtccagccg gtcgatggtg ttggtgagag caaaggcttc cggcaccgaa
    10021 atcggtacga gcatccggca gcaggcggcg cagccctttt cgcacgacac cctctggccg
    10081 gtttggagta atcggcgttg ttccaggacc tgggcttctt ctccgagact gcgcatgagc
    10141 gggagaatcg cagtgatcgg tacgaagctc gttggcacgc ccacggcagt gctgatttct
    10201 ccgcttggag tctgcagaga gatttcaaag tgttcaacgg cagtgtggtt actcacgggt
    10261 acccatcttt aagtccgaat gggagtcact tgcgtgatgg catttgtgcc atcacgcgca
    10321 ggcgctatca tagagaacct cagaatcagt ggtcaaccaa agcctcatga ccctcagtct
    10381 tgccccggga ggactgttgc ggctactctg tcgtcgatgc gtttgtcagg ccacattatt
    10441 ggattactga aagaatacat gcgggatctt gtcgagcaag cgcggcagga gtcgcaggct
    10501 caggaggcct tcggcttctc ggcacctccc taccagccgg accatgccat cgctgatttg
    10561 ttggcgattt tagacgaccg tatcgagtcg gaaggtgtac aggtcggaat gccgctcgag
    10621 ttcttgcatg aaatgtggcg ccgctgcaat gaggcgcgca acgagatcgc cgaacgcgtc
    10681 tggctcgaac gcagccttga cgcgcccgat gtctccaagg cgcgcatcag agaactgaca
    10741 tacgcttccg tgatcgagta tctcgagaac gagtctctcg gagactgaca atgcgaggga
    10801 ctggattacg atggcgtcgc ctggctctcc tgatgctggt cgtcatgacg gcgatcctct
    10861 ccgatgaaac aaaggccagg ggccagactg atacgccagc caaccggccg tcgctcacac
    10921 tgagtccgat tagcacccaa ggtctcaggc agccgctcta tttgacccat gccggggatg
    10981 gaagcggtcg tctttttgtt gtggaacagc ccggtcgcat ccgcattctg accgaagaca
    11041 ggttgctagc cgtccccttt ctcgatatcc aagaccttgt gctggccgga ggagaacgcg
    11101 gcctgctcgg ccttgccttt catccggact atgcgcacaa cggtcggttg tttgtgagtt
    11161 ataccaggca gccggacggc gcaaccgtgg tggcggaata ccgccgaggt tccagggagg
    11221 atcaagcgga tcgccaggag cgggtggtaa tgatcgtgca gcaaccattt gccaaccaca
    11281 atggaggcat gatcgcattc gggcctgatg gcgcgctcta catcgggcgg ggcgatggcg
    11341 gcggtcgtgg cgacccggat agtcgcgctc agaatccaca ggacctgctc gggaagattc
    11401 tccgtctcga tgtgaatcaa ggtgagccct atgccattcc tgccgacaac ccctatgcgt
    11461 caggtggagg ccgccgggaa atctatgcga tcggtctccg taatccttgg cggttctcat
    11521 tcgaccgtga aaccggttcc ttgtgggtcg ccgatgtcgg acaggacgcc tgggaagaag
    11581 tggacctggt ggtgcgtgga gggaactacg gctggcgcgt tatggaaggc cggcattgtt
    11641 ttctcccgaa aatagattgc gacaagccag gactccagct cccggtcctc gaatatgggc
    11701 atgaggccgg tcgttgttcc attaccggcg ggtatgtata tcggggatcg agccaaccgg
    11761 cgctcaaggg tctgtatgtc tatggagact actgcagcgg agacatattc gcggcgcggc
    11821 tggaaggcgg ggcgcatccg cgcgtgattg aggggccaga gatcttgtta cgatcaggcc
    11881 ttcggatctc ctcttttgga gaagacgagg cgggcgaact ttatgtggtg gatcatgggg
    11941 gaggggtcta tcggttggag tcgagtcgcg aatcgaggcc ttacagcttt ccggcgatcc
    12001 gatagacgag gattccgaag acttcatcaa tggcctgcgt ggtgatcagc agcgaccttg
    12061 ctgtgggaat gaaatcaaag gcagtcgctt gttcccaggc ttgttgtgga gtgtgttgag
    12121 attcgtagcc gcagggaaac ggggtgacga caaaaccttg cttttcgaag agtctgactg
    12181 cgcggggcag gtgataggcc gcggtcacca acaggatccg tcctgaccca agcatggcct
    12241 tggtctgcac ggcgttctca taggtggtcc tggatttttc ctcgatcagg atcgcggatt
    12301 ccggcacacc gaggcggagc gcccaccgtt tcatctcgtg ggattccatc agaccggttc
    12361 ggaatacggt ggcgtcgccc ccggtgagca acagtttcgg ggccagattc tgttgccaca
    12421 agcctgcgcc acaacccgta cgctggcggg aggcatcaga gacctcatcg gtcgggcgga
    12481 gtgatccctt ctgaaagacg cctcctgcaa ggaccaccac cgcgtcgaac ttcgatgtgg
    12541 aggtggcctg gaatggtgga tactgtgctt cgagcagcgc aatgtacatg ctggaaagaa
    12601 tcggggtggc cgtcaggaac agcagcagca cggtgaggaa tgagcaccgt ctgacccaag
    12661 tcctcctccc gggggacacg gggagacagg cggtcaacag ggcgaggagt ccggcgatga
    12721 gaatccagga caaggggtag agtaggtatt tcaaggcctt atacacaccg aaccagatgg
    12781 gcgtaaggtc catagacgct cccggttaac aggcgaaaca gagtgctcag catacactga
    12841 gggcgtacag acaccaagtg ttgaggagga ttcgatggca gacggacggc actattggtt
    12901 gatgaaatcc gaacccgagg tcttttcaat cgatgatctt gctgggtcac cgaagagcac
    12961 gacttcatgg gatggtgtac ggaactacca ggcgcgtaac tttatgcggg caatgaaggt
    13021 cggggaccag gtcctctttt accacagtaa tgccaatcct cccgcggtgg ttggagttgc
    13081 ggaagtggtg agagcggcct atcccgatgc cacgcagttc gataagcggg attcgcacta
    13141 cgatccggcc agcaatccgg ctcagcctcg ctgggacatg gtcgatatcc ggtttgttcg
    13201 caaattcctc gcgccgctgt cgttggacct gttgcgtgcg aaggctggtc tgaagggaat
    13261 ggaattgctt cgaaagggat cgcggctgtc ggttcagccg gtgcgggcgg ccgagtggaa
    13321 tgaaattatc aggctggagg aggtacaggc ggacagctag cacggtccgg gaagaactca
    13381 cttccggacg ggaggtgtcc tcggttcagc acgggtcaac tacgagcctt tccatcatcc
    13441 agatatcgat gttgccagtc gagccaggca tgtcccagtt catgggcgag aatgtatctc
    13501 cgccgcgtga cgggtagtcg tttgcgaata tagatggtct tggcttcatt gtcccaaatc
    13561 ccatccgcat tcgaatcctg ccggtccatc tcggagtccg tgacctgcct gatcatgatg
    13621 tggtagccga atggaaggat gactttgttt ggtattcgca ccatgcccct cacggtcgta
    13681 tcaaaatagg tggcctcagc caagaattcc tgctccggaa tagcctaaca tatttcctct
    13741 ttcctcttcc tgtctaaata gtaagcatta tcagttactt gcgggatatc gctgcttaat
    13801 cgctgtattg cggctcggtg gtagttgagc aattttcgat ggtgatgaga cggtactgag
    13861 gatttcaatt gaaagattgg atggtgtatc atgcccccat ggttgagaaa cttcttgcgc
    13921 aagaactcgc gaacccctat cccgcagtcg ttcggatcgt gggcgtaaag atccgtgacc
    13981 gtggagaggt gaagaaattc gatgccggag aggcgtcgct ggtgatgggg gatcgggtgc
    14041 tgctcgaagt ggccggtgag ttgagctacg gggtcgttta cggggctcct caggtgatgc
    14101 cgttcattcc gcccatgcgc gtgctgcagc cgatcactag gaaggcgaca acggaagatg
    14161 tggccacgat cgaccggtac gaacggttgg catcggaggg aatgaaagcg tgtcgggagc
    14221 aggcggcggc cctggggctc agaatgaaac ttgtggaggt gttctgctcg tttcatcgcc
    14281 ggcagatgac ctttgtctat accgctgagg atcgaatcga tttccgcgag ttggttcgct
    14341 tattagcgcg ccgattcggc ggacgcattg aaatgcggca agtcggggtg cgcgacgaag
    14401 ccagtcggtt gggggggatc gatacgtgcg gtctggtgct ctgttgtgcc gcgttcctca
    14461 ctgaagtgaa accggtgact gtcaagcagg cgcgggtgtt ggggcttcct gtcgatgacc
    14521 ccaaattact tggtgtctgt ggtcgtctca agtgttgcct cttgtttgaa gcaatggaca
    14581 atccctcctc aaaatcctcg gccctgatta acccgtcgcg ttctcgcttg gcttcttcgt
    14641 aacccccagc agttcacaag agtcttgctc tgattcgtct cctcggcccc gctccgtcct
    14701 cgcctttcgt tgagaaaagg cggttctttt cgtccgaagg ggtataatag gctgtgaggt
    14761 gacggatgcc tcaggtgttg gtgcttggag ccgggaaaat cggatccctc gtggctgggt
    14821 tgctgtcggc taccgggcag taccaggttc acctggccga tcttacgctt gatgccccga
    14881 agcgactggt tgatgagttg tcgttgtccg cagtcactcc ctgtgcgtta gacgtgcggc
    14941 gaccagatct agttgcgggc tatgtcggtt cccaccgatt cgacgcagtg atctccagcc
    15001 taccgtattt ctgcaatccg accgttgccg agatcgcgcg agcccatcag cttcattatt
    15061 tcgacctgac cgaagacgtg gctgtgactg gtcgcgtcaa ggcgatcagt gagggcgccg
    15121 atcgtgcgtt tattccccag tgcggcctgg ctccggggtt tatcagcatc gtgacgaatg
    15181 accttatcgg ccattttgaa tccatcgaca acgtgaagat gcgtgtcgga gcgctgcctg
    15241 ttcatccgag caatgcgctg aagtactccc tcacctggtc caccgacggg ttgatcaacg
    15301 agtatgccaa cgtctgtgtc ggcattgaag ggggggagga ggtccacctt cagccgttgg
    15361 aaggctatga gacaattgaa ttggacgggc tgctctacga ggccttcaac acgtccggcg
    15421 gcctgggcac tctcgcggac acgtaccgcg gacgggtccg aaccatgaat tataagaccc
    15481 tgcggtatcc cgggcattgc gaaaaaattc aatttctcat gaaggacctc aagctgaatg
    15541 aggatcggga gacgctcaag cgaatcctgg agagagcgat tcctcagacg cagcaggacg
    15601 tagtgttgat ctatgcggcc gtgaccggca ccaggcaggg tgagttgttc gaggagacct
    15661 atgtgaaaaa ggtgtatccg cggacgatcc tcgggcggct ctggtctgcg attcaggtca
    15721 cgacagcgtc gtcgctttgt tgcgtcgttg atcttgtgat ggccagacct tcagcttatc
    15781 gtggctttgt cacgcaggaa cactttcact tgcaggatgt gctgaacaat cgcttcgggg
    15841 attgtttccg ggcctgacgg catcatgacg atacaggaac tgttcaagac cttggggttg
    15901 acgcaggaac agcccggggg atgtctctcc tcttcgacgt ggagtcgcac caccgacgcc
    15961 ggtatcgtcg agtcccgcaa tccatcaact ggagaaccgc tggcgcaagt gtacgggtgt
    16021 tcccttgccg attacgatgc cctcgtagcc ggatcgcagc aggcccaggc ctcgtggcgt
    16081 atggtgccgg cgccgaagcg cggcgaggtg gtccgtctga ttggtcaggc gctacgcgtg
    16141 cacaaggatg ccttgggtac gctggtgtcg ttggaggtgg gcaagatcaa ggccgagggc
    16201 gatggcgaag tgcaggagat gatcgatatg gcggacttcg ccgtcgggct gtcacgcatg
    16261 ttgtacgggc agaccatgca ttcggagcgt gcacagcatc ggatgtatga gcaatggcat
    16321 ccattgggtg tggtgggtgt cattacggcg tttaactttc ctgtggcggt ctgggcctgg
    16381 aatgctttta ttgccgcggt aaccggcagc accgtgttgt ggaagccgtc accgaaagct
    16441 ccactttgtg cattggcggt tcagcggctg tgcaatcgtg tgctggaaga ggccgggtat
    16501 cccggtgttt tcgcgctctt tacgacggat cgcgtcgagc tggccgagcg gatggtgcgg
    16561 gacgagcggc ttccgttgat ctccttcacc ggctcggtgc ccgtcgggag gcacgttgcg
    16621 gaggtggtcg gacatcggct gggccggagt cttctggaat tgagcgggaa caacgccgtc
    16681 atcgtggacg aaacagccga tctggattta gcgacgcgcg ccatcgtctt cggcgcggtc
    16741 ggtacggcgg gacagcgctg caccagtacc aggcgactga ttgtgcatga atctcagtac
    16801 gagctgctgg tcccgcgact gctgtcggcc tatgcgcagg tgaaaatcgg tgatcccctc
    16861 gagccggata tgctgatggg gccgcttatc gacggggagg cggtgacgcg ttacctgtct
    16921 gcgcttgagg aagtgaagaa ggcgggcggt gaggtgctat gcggaggatg tgtcttgtct
    16981 cgccccggat attttgttga gcccacgatc gtgcgggcgc agaaccactg ggatatcgtc
    17041 cagcgggaaa cctttgcgcc gattctctat gtcatgacct atcgaacgct ggacgaggcc
    17101 atccggatgc acaatgcagt tccacaggga ctgtcctccg cgctgtttac cacccatctt
    17161 cggaacagtg agaccttctt gtccgcgatc ggcagcgact gtggcattgc caatgtgaac
    17221 atcgggacgt ccggtgcgga aatcggcggg gcattcggtg gagaaaaaac cacgggtggc
    17281 ggacgggaag ccggttccga tgcgtggaag gcctacatgc gtcgacaaac caacacgatt
    17341 aattggggaa ctgagttgcc gttggcccag ggcgtgacgt ttggacgggc ggaaggaggg
    17401 aatgatgggg caagaagctg acctcgatgc ggtgatggct cgtatgacgg ccgactttct
    17461 ggcgcagaat agcgcaggac ggatgcttcg ccgggcgctg gacgacgtcg gggttgggtt
    17521 tatcccggtg atcgaccatg tcaccatccg gacgatggat attgatcggc gcgccgagga
    17581 atttctcgca ctgggttatc ggtacgatga aacgcttcat tatgacgatt ggtatgccaa
    17641 agtattccgg tgggccgggt atccggcgct gtttgtcgat caggcctatc cggacgaccg
    17701 tggcaagacc agcatcatcc cacgctgggt cgagaaattc ggtgatcagg tgtttcacca
    17761 tgtggcggtc cgtgtcgaag acatcgagca ggccattctc aaattgaagg cgaagggcgt
    17821 ggtctttgcc ggcgagatcg tcggtgcccg gggaggcacg ttgcgccaaa tttttacagc
    17881 ccctgagatg ctcgatgggc aacccttttc cgtccttgaa ttggccgagc ggcaccaggg
    17941 ttatcaggga tttcttcctc cacaagctga cagtctcatg cgctcgacaa tgcgaggcct
    18001 gtaaggcagt aggcttcggc cgaccgctgc tctgctgtcg cgatgtgttg gcagcctctt
    18061 cccgccgccc aaagagtgcc tcgaccagat gggttgaatt gcgtacaatg cgccgtgtcg
    18121 accaaccagg tcgagagtgt ctggcatgcg cgatctcaac aggaggttct cttcatggtg
    18181 caatctaacg cgacgttacg cttgatcctc gcactgctgg tgctctgtgc acttggagca
    18241 ggaagtggtt ccgtgcaagc atcgccgtcc ggccatgccc ctcatggcac cgtgacgatc
    18301 gatggtgtca cggtcccgga tgtcgggccc ctgccgacgg tggtcccgat accgtcctcc
    18361 aatctcaact acaccgctaa gatcgaactc ggcaagcaac tctattttga cggacgactc
    18421 tccaaaaata acgcgatctc ctgcgccttc tgccacaacc ccgggaccgg atttgcggat
    18481 ccccgccaga cgtccatcgg agtcggcgga ggcgtcggag gacgacagtc gcctacggtc
    18541 tacaatacgg gattgaatca cgttcagttt tgggatggtc gggcacgttc gcttgaagag
    18601 caggccatcg ggccgattca taaccccgtg gagatggccg agacgcatga acatgtggtg
    18661 gccaagctgg ggaagatcaa agggtatcag caacaattca gggctgtatt tggaacagat
    18721 gtcaatcttc agggcattgc cgaggcgatc gccgcctatg agcgcacagt gctctccacc
    18781 aattccgctt ttgataaata cgtgttaggc gcgcagaaag ccatggatga ggcggcggtt
    18841 cgaggcctgg ccctttttaa gggccaggct cgttgcatcc tctgccacaa tgggccgaac
    18901 ttcaccgata atcagtttca caacctcggc gtgcctcagg tcggtccgat gaaggaagac
    18961 cttgggcgtt ttgctgtcag cagggcggag aaagaccggg gcgcatttaa aacgccgacg
    19021 ctccgtagca tcacagaaac cgctccgtat atgcacgatg gcgcgtttaa aacgctggaa
    19081 gaggtcgtcg aatttatgga tcagggcggc gggagcaatc ccaatctgag ccccctcgtc
    19141 aagccgttga atctgacggc ggaggagaaa agcgatctcg ttgcattctt gaaggcgctg
    19201 gcaggagagc cgatcccgtt cagtatgcca aaattgccga aataaaacac gaatcctttt
    19261 gggccgcctg tgactctcac gggcagatac gtgtgccttg gtcagactat tttgtccaag
    19321 catcggtacg ttccaacccc aaccatagga ggccatctat gtttcgaaat ctttgtgtgc
    19381 tcggtgcgag caccattgcg ctcgccctcg gcgggcctgc acttgcggcg gatatgaagc
    19441 cgggtccggc ggacggattc gacattcacg tcatggcgcc ccataaaatg gaagatgggt
    19501 cggtggcagg cccgttccat cactattgta aggccgtcaa gccggaagtc ttgcaatgtc
    19561 tgctgttcga atccacggat cccaatgccg tgctcaccga cgtggagtat tttgtggcca
    19621 agcctgtgtc acggtcggcg gtgcctctcg atgtctggaa caagttctat cacgaccatg
    19681 aggtcgaaat agcgaccggg cgtgttcagg tgctggatat gcctgatgct caggccaaag
    19741 aagtggcggc ggctgcggcc aagacggatg gcattatttt ccacttgtgg ccgaagggtg
    19801 cgaaggctcc ggatggatcg gtggagcatc cgacgtccgt ggggcataaa ccgcggacgg
    19861 agtaagtctg cgaatagcgc gtgatccatc ctgtgcccta attgtaagga ggccactatg
    19921 cgaagtgcga ttttgatgtt tggattgtgt gcggcggcgt tgacgacagc cggttgtgcg
    19981 gagactgccc gcgaaatgaa atcctcctct gcggcaccag ctgccgccat ggcggcgaaa
    20041 gctatgccga caccggcgca gggctatacc attcatgtca tggctcccca caaatttgaa
    20101 gacggatcgg tgcatggtcc gtatcatcac tactgcaagc cgatttctcc ggaagtgctg
    20161 cagtgtttgt tgttcgagtc gacggacgcc aacgccttgc tgacggacat tgaatatttc
    20221 gtggcaaagt ccgtctctcg cgcgcatgtt ccgttggaga cgtggaataa gtattaccac
    20281 gaccacgagg tagagattgc cacagggcgg gtgcagattc tggacatgcc ggaggcgcag
    20341 gcgaaggaag tcgctgccat tgcggccaaa accgacggga tcatttttca tctttggccg
    20401 gacggagcca aggcgccgaa cggtcaggtg ggacatcccc agtctgtcgg ccacaagcat
    20461 cgtactgaat agccggagcg acgcagatcc atcgggcgga gggggcgcag ccctctccgc
    20521 ctatgctatt tctgatgaaa gggagacgct gtgatggatg ctggacgtct atgcctgatg
    20581 ggatggctgc tgttcggcgg tgtatctgtt ggttgggcgg cgaattccga agtgttaaga
    20641 ccccgtgttc cgatcgacca gatcgaggcg gccaggactg taaccaatcc ctttcctgtt
    20701 acaccggaaa cgctccagca gggaaaggcg ctcttcgaag gcaaagcatt ctgtcgcgcc
    20761 tgccacgggt cggatgggaa gggactagga atggatttgg actattccac cttcaaagga
    20821 ccactcccac ggaactttac cgataggcta tggcagcagg ctaggacgga cggtgagctg
    20881 ttctggattc tgaagaacgg gagtcccggg acggatatgg ctccgtttat tccactcgtc
    20941 ctgaccgagg aggaggcctg gcaagttctc atgtatgtcc gttctttcgg tgggcgatga
    21001 cgtgtgagcc ccgcgccgca ctcacggttt cagtgtcgtc gataggctcg gttccttgcg
    21061 ggcggcttgt ttgtcgggat gccgcaagct cgccgcgctg agacacaggt gaatggtcgg
    21121 cattgaccga agctgaggcg ctgtataccg acaatgtgtc tgactggtcc gttacgcggc
    21181 gtccgcacta tcaaaggatc tctcagccag ccgacgatca ccccccccca cttcacaaga
    21241 tgttgccggg agtctcttga tgtccggcgt gtctcgagga ctgcttttgg ccagaccgaa
    21301 tgctcgatga acgcaaccgg cgtcatcaag accgatcata gttttcttat tcactgaaat
    21361 tatcgattgc aggcaagaag gcgttggccc cagattggat cgctttttgt gcctggtgca
    21421 gggtttctct tcgtatcttg ggtaggtcta tcgacggaag agctttacga ttgagctggt
    21481 aggagatagc aatcgggtcc tagttgagac gacctctgtc ggatccgcgg aactgcattg
    21541 tcggattggc ccagccgcgt tgggacccag gcattgcagg gggatcggcg agccttcaat
    21601 acggcggtcg tgattttata gtaccaacac ctccgtcggg atacagcatc gactttgggt
    21661 tgtgaggaca ggcgtttgtg aaggtgttgt tgagacgtta gaagatcagc gctggtgggc
    21721 caataggaaa tgagctaagc gggcctgatc agaagtaggg agcttgagga attcgatccc
    21781 gaagtattcc tccctgatcc atcggacggc ggcgagttca atgatcagtg cgggggtggt
    21841 atcaggcagg agcaggcgca cagccatata gctaccctcc aacattctcc ggtcgcattc
    21901 cattaaacat cccgtttgag acaggttgtg cataaggcct tctccgataa agagggcgcc
    21961 ggagaacatg acaggacaga cgattccaaa gcggtctcga gaccgctgaa atatgagaat
    22021 tgcttcggat gaaggtgggc ggtccagagg gccaagtgat gctggggagt agtggctcat
    22081 ggaagggagt atagttccga gacgcagtgt ggcaacatcc cgccttggag gggccagaga
    22141 ctcgttggat gaagtcgatg cgggaattcg aatgacttgt gatgacaggt aattcaggga
    22201 cagttcatcg gcggcttgcg gcataaaagc ggtggctgca ggcccgacac cgccaagggt
    22261 aacgaccaag cagataccgg acgaggtctc gccaaccccg acgttcagag cgacggatga
    22321 ttccggtaca ggcggggcag gaataagctg tcttggacag ggtttccagt gagcgccgat
    22381 cgcccaagaa agagatcatt tagtgagccc ccgatttgag agcttcgcac tggtcgcggg
    22441 agtagtcgtg tagcggttca caggctgcag gtttcgggct ttcccagttc gccatgcaat
    22501 aaaatcgctc tcgatagctg atggtgagta agtcgaaggg gctgggtcga gggtgttccg
    22561 tctgttcacg tcggcggtcg gtcgctgtct cgttgtgagc agcgctcaga tgcctggcgt
    22621 gaggacgcat tactcctgcc accacaccag gcgaatgaga tagattcctg ccacataagt
    22681 tcccgcaatg acgatggccc agagcatgat cgtgcctcct ctttttatct tctctgtttt
    22741 tgccggagtg tcgctgccat aacgactctg agcagtagca actcgcgtac cactttcgta
    22801 atgcgtccca atctcggtta aacacattga ttcgcttgtg atgagcgcct gtgagatcaa
    22861 tcgcgttaag gcagcaggtc taggcaaatg ggaccaaggt gtatccgatt cgataatgac
    22921 agcatcaatt tggataacgt tgggaagttc ggcttgaatg tggccgtggg ttggctactc
    22981 gtggctgggc gaattcatgg tgtgggcggc cagaattgcc gtgaggaaca tggctctgat
    23041 gagccttgga tggcacatac ttacttcgcc tgggagtgaa agcggtgacc aaactggtgc
    23101 ctgcctcgat caaggcgcgc ctggattttg gtcgccggag ctagttcgag cccagaagtg
    23161 aactcgcaat tgcgaccaga atcatgaata gcgatgcgat taagctgagt gccgccggca
    23221 agtcactctg tgctctgcgt aagaggtctt ttcggacggg atttttctca gccattatga
    23281 tcgtagttga attacgcgag attctgagct ggcgaagcgc ctttttgagg ttgttgttcg
    23341 acggccaagg catgggctgt gatagcgtag ccgcagtggc cgcaagataa aaatccaccc
    23401 gtgggggcaa gactgtgatg tttgcagcgt ggacaatcat agagcttcat agtggtttcc
    23461 tggactagtg gcagaaatca ttgtgtagcg tcgggattgt gccggatggt tgtggctgcc
    23521 gtaactcctt ataccgtgcg tgtaggtgcg ccacggcgtt ggcgagcgac gtgaactcat
    23581 tggactcaaa atgtagggtg attaagccat acctgaagtg aagttttccg caggtgcaca
    23641 tgttgagtgc cgtctggccg ttgcgcggat tgagcgaatg catgggttgg ctgctccttt
    23701 gttgtatgag agggcggttt ctatgatcgg tcgtaaaacc ggctgttgat tcgcaataga
    23761 gactcatccg ggttaggact agggatgtcg tgtgattcgt agatcttcta cagttatgtt
    23821 gggtatggtg cggcgtgatc tcgcctgcct ctcgcgtgat gaatatgagc gaagtaagct
    23881 atgggccccg caattgatgc atctccatcg gtcctccgga tgatagaagt cgttggatgg
    23941 ctcgctggca agataccctc cgcaacgaga acaactcata cgttgcccct ccagaataaa
    24001 gtgttctatg tgaagccgct ggacggagac ggtaaagggg ctccctccac agtctccacc
    24061 agcgggtcca ttcacgctac tccgtcgacc tgatgggctt gatcagtgcc gccttgtctt
    24121 caggcgaggg acataaacgg gcagttcctg gtcgcgctga agattggagc agggcgaatg
    24181 actattatga tttcaattct caatatcagt atagtgagtt cgtgagatct cgggaatgct
    24241 tattttcctg atcttcagat aatttcttgg tgctgaggat ggcgcgggcc tgctattcga
    24301 actgtgaagg cgaggccctt ctagcaggga acgaaaaaga gggagctaaa acgcggtttg
    24361 ggaggagtgc ggggggcgcg gccggctcag tcagtgaaac ttcttcatct cctagccggg
    24421 agagttgaat ctacgccacc ctcagcatga gaaggaggaa cgagtccggt catctgtggc
    24481 gctcgttgcg tcttgcgctc gacaggcgaa aatcgagggt ttccatttcc acgacgtgcg
    24541 acatacgttt gccacgatcc tgttacagag cggactcgat ctctacaagg tgcaatagtt
    24601 gctggggcac aagtcatcga ctatgacgca gcgatatgcg catcattatc tggagagttt
    24661 acgaaatgag gtagaagttt tagatcgtat gcgaggagct aggacaaatt tagcacagtc
    24721 ggcgggtctt gaagaggggt tgaactgcgt aggtgattgg aaataatggt gggccgtgta
    24781 ggggtcgaac ctacggcccg ctgattaaga gtaccgacga aaccctgcct acgctgacct
    24841 accctgagga atcagaagcc aataacgagg atgaagacta ggccattctg ttcttagatc
    24901 gtgagtggtc atggttcttt ggcacctgat tggcaccagt taagaacgtt ggatggctcg
    24961 acgtaggttt gctgcctcct catcaagacc actttcaagt gtgccgctga ttgcctgtca
    25021 gattgccctc tacaagtctt tgattctccg ctcctattcc gccttggtgc caacttggtg
    25081 ccgctaaatt aaaagccgtg actggtgccg aaaaggctgc tactaccgct ttggatgatg
    25141 tatccggacg gtatagcaaa cctgtgcggc atggcctagg gccagaaagg cggaaggtag
    25201 tctatgcatc acatcgttga agacggggcc gaatgtgaac atgtggcggg gtgctcttcg
    25261 tgtcgaaacg gggaaaccgt gccttttgag tgttcagcaa agttgcgcgg cgaaatcgag
    25321 cgccgagctg ccttctatgg gcgaacgtgg gacgaggaat taaaccatat cctcgcgatc
    25381 gcctatgaac agcgcgagcc ggatgtagac gatgaagaca tgacgcgacg tctgaacgag
    25441 cttcggcgcc gtggggagtt accgagtcag aggcgggcga aagtcgaaat ccgcttggcc
    25501 tccatcgatc gattgccgga cggcgttgac gtgcctcgcc ctaccacagt caaagagctc
    25561 tgcgacatct atcttgagta caagctgaaa ggccggcgct cttatgccaa tgtccgccga
    25621 acggtccggc acacgatgga acagaccggc tttgccgatg tgaaactgtc tgatctcacc
    25681 tcgggtgtaa tcaagcgatg gcatgccgga atggcctcac gacatgtgac gggaaacaat
    25741 gggctgaaga cgctccgttc cgcctatcgg ttctgccgga gtttggaatt gctggacagt
    25801 gatccgaccg ctggtatcaa acgattcagg gaagacgccc gcgaacgata tgtgacccct
    25861 agcgaaatgc ctcgcctcat gcatgcgctg gagggcgcac ccctcagggc acaaacgttc
    25921 ttcctcacaa tactcttcac ggcctctcgg cctggggaag tcgccggcat gcagtggaag
    25981 gatctcgact ttgttggtat gcgttggcgc aaaccgacta gcaagactgg ccgctcacaa
    26041 gaggtcgtgt tgaatccgat cgtctaccga ttgctccgcc agctgcctca ggtttccgac
    26101 tatgtgtttc cgggcaagga cctttctaag ccctggaatg aaagcacgca gcgcaaggcc
    26161 tgggatagag tccgatcggt tgccaagctg ccggatgtaa tcgcctatga tcttcgccgg
    26221 acaatggcta gctggatggc gcaacacggg ctcaatcttt ccgtcatcca acagagcctg
    26281 aaccacagca cgctacaaca tacctctcgc tatgcgcggc ttgacgtcca gaccgcgggc
    26341 cgtgccttgg atggcgtctc caagcacatg gctgcctcga atcgattctt taacgacctc
    26401 tgcgcgcctc tcacatctga ggagctctct ccagaggcgc aagagatggg cacaacagcg
    26461 gagcaagttc aggctcgaca cactgagaca tcctcggcgg acgagtcggg aacggagccg
    26521 gaggatatgg aaattcccgg ctagctcagc aatgagcaca ggcgaggggg tagaacgccg
    26581 cctcctcgcc cctggcttga gtcgattctg aatgaacgaa gagggagggc ctcaccatga
    26641 aaggtttagg tgccgtacta ctgggcagtc ttctgctact gcagggctgc gccgggctga
    26701 ttctccggga acatgattca gtcggcacca aagcggcgaa ggttgcggcg cgggttctag
    26761 cgtggccggt gacgttgacc ttgagcgagg tggtgatcgg cagtatcaag caacgcgagg
    26821 agctggaagc cagccaacaa caggcccagc tgagtcaacg gaaccatttc gatggacttg
    26881 tcggccggct gacctattca gaggcgctcg tgaggtgggg acctccggca cagtcacagg
    26941 cgcacggtgg gatgattgtt gccgtgtggc agagccgccg ggcgagtcct ggctatctga
    27001 tcatgccgcc gatccctggt aatccttatt cctcttc
//
LOCUS       DAORQN010000009        26905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_124_length_26905_cov_25.491136, whole genome shotgun sequence.
ACCESSION   DAORQN010000009 DAORQN010000000
VERSION     DAORQN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26905
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_26905_cov_25.491136"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1085)
                     /locus_tag="PK614_01475"
     CDS             complement(174..1085)
                     /locus_tag="PK614_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB80914.1"
                     /translation="MQKPSVFAAYAALTTSALVWGGSIVGQKLALGAFSVVETSLLRG
                     FGALVILIPLWWWSEGGRTTWTARDIKLLSLLGLGVLGNHLLTLFGLRYIGAATAGVI
                     IGASPAITALLSSLLVRDIPFNTVAAGCAVSFAGVALVSGVGGEAPSGDNPWLGGTLV
                     LLGLVSWALYSIGGRQVMERLSPLTVNWTTLLLSLLLQIPLLWTDQKLLLTGASVVPV
                     SGWVALGYLIVFATALGQQAWLYGVQGVGPSRAGVFVNLIPVSALLLSALVLGEAIGI
                     REIVGIALILAGVWLVGWQSVRLKQTG"
     gene            complement(1145..1795)
                     /locus_tag="PK614_01480"
     CDS             complement(1145..1795)
                     /locus_tag="PK614_01480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB80915.1"
                     /translation="MTDLVLPKIDAYAAAYSLPETPVRRALREETERTMEFARMLVGP
                     LEGAFLQMMARLVQARRVLEIGMFTGYSALCFAEALPEQGQVTTCEVDDASAAVARRF
                     FAQSPHGRKVEIRMGPALETMAGLTGPFDLIFIDADKLNYVNYYRRAMELLSPRGVIL
                     IDNVLWDGDVLLEPPPDDRTAAIQELNRVVRTDQRVSAVLATIRDGIWIITPTPSA"
     gene            1882..3402
                     /locus_tag="PK614_01485"
     CDS             1882..3402
                     /locus_tag="PK614_01485"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRB80916.1"
                     /translation="MKTLATLEPLTTRLLEIRRIQSAAAVLSWDQETYMPAGGGAARA
                     EQIATLEGLAHQRLVSAELETLLTEWIDPATGQAADSWDEPSRSLLRETWRDFSRAKK
                     LPSDFVIRLSRECSLAQQAWVTAREESRFSKFLPSLKIILGLKRDEARYLGYRNSPYD
                     ALLDTYEPGATIAQLAPLFTQLRERLVPLLRRVQASHVTIDDRCLHQSFDQSKQVEFG
                     RLVLTAMGYDFERGRLDLSAHPFTTSFHPTDVRVTTRVFEKDLPSCLFSCIHEGGHGL
                     YDQGLDPRYYGSPLGESVSLGFHESQSRLWENCVGRSRAFWHCFYPMLQHTFPQQLAD
                     VPLDRFYAAINRATPSLIRVEADELTYNLHIMLRVEIEQALIEGRAEPDDLPGLWNEK
                     MQSYLGIVPERDAEGVLQDVHWSMGAFGYFPTYTLGNLYSVQFFEQATLELPQLEEEM
                     RAGHLLPLRRWLEQKIHRWGRMFTPDHLARRVTGSGVNPEPFLRYLETKYAELYRL"
     gene            3891..4595
                     /locus_tag="PK614_01490"
     CDS             3891..4595
                     /locus_tag="PK614_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB80917.1"
                     /translation="MSGTLAVDACQDSHPAKAEVRILLADDHALLRRGLREFLRDFLV
                     EDGLTPDIVETASARHAIDHIRTGTWDLVILDLNLPDMPGLEVLRILKSLRPALPVLV
                     VSIYAEEHYSARAIRAGALGYLTKERGPQELRMAVSRILQGGHYLPPPETEHNQDEGP
                     HRNLSSTDPLPATLSDREIEVLQWIAQGRRLTEIAEHLNLSIKTVSTYRSRLLIKLGM
                     RTTAELIRYALDQQLV"
     gene            4726..5112
                     /locus_tag="PK614_01495"
     CDS             4726..5112
                     /locus_tag="PK614_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80918.1"
                     /translation="MANILIINADANLKRLIKLALPGMEHSVQHSATLQTACAQSTSS
                     KLDLVISRFVADDDQGPKDLMELRKTFPTARLIVTATLHDPALEGEKFQRVVRELHIE
                     YCLLEPIETGAMLHAIQSALALPKRP"
     gene            complement(5200..5763)
                     /locus_tag="PK614_01500"
     CDS             complement(5200..5763)
                     /locus_tag="PK614_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80919.1"
                     /translation="MRKSHEMTIDRVLLLYALSLAEPYGLLSDVKLQQLCFLCELQMF
                     GKGMKGFHFEFVRFAYGAFSRDLDNDLTSLRRKERVENFSPSDQVRDEVLPLLMKGID
                     GNETNEKIRDLMQAVITTYGPQDVTAITQSVESVELSTPQQPDFKIPIRDIVFHTTLL
                     VPARIEASAEFLLPPSQLAKLNTAMGY"
     gene            5917..6348
                     /locus_tag="PK614_01505"
     CDS             5917..6348
                     /locus_tag="PK614_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRB80920.1"
                     /translation="MSKTLKEMDVLRQHLAKHQLKLTRQRELILTAFLRQEHVTAETM
                     YHQLAKKDPHLGLATIYRTLNLFCEAGIAQARHFGTQTQYDNISHKGHHDHLICTGCG
                     TIVEFENCEIERLQEEVATKNGFVIQTHRLELYGLCARCRH"
     gene            6444..7475
                     /locus_tag="PK614_01510"
     CDS             6444..7475
                     /locus_tag="PK614_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRB80921.1"
                     /translation="MASSCRTFLALTLLLGALTFLQWATGTAEAAEKLVVYSGRAERL
                     IKPVLDEFQAKSGIQIELLSSGTTELVNRLQAEGDHTPADVFLTNDAGSLEHARELKL
                     LRPMNMREVERAIPSQFRAADNSWIGLSGRFWIVVYNTNLVKPDQIKSLFDLAQPQWK
                     DKIAVPNSGSEYLQAGVSVIKATFGDERTKQFLQGLKANAGTQVYQKSSQIVDAVAKG
                     QVAAGIVNHYYIYRHLATQPTAPIAAVMTDQQEGGMGAIMNVTGIGVTRASKHVESAK
                     LLIEFLVAQAGQKMFADLDKEYPLHPDVKADPTLIDRRTFRAAQVPLARLAELREATL
                     TLIEQVGLR"
     gene            7494..9098
                     /locus_tag="PK614_01515"
     CDS             7494..9098
                     /locus_tag="PK614_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRB80922.1"
                     /translation="MLTATRAHAPSSLQWISLITAAFLVLPTCYIIYVALTAAPTVWS
                     RLWSTRIPELLWNTLSLATGVALTTLLLGVSLAWITVRYEFPGRRVWEWALALPLAMP
                     TYVLAYVYAHLLGMGGPVEQGWQTLMGSDARLLSPQSFVGVTLIMALDTFPFVYLLVR
                     GALLNLNISFEEVARVCGVSPWSTLWRVTLPLIRPAIAAGLALVILYVISDFGAVSLL
                     RYQTLTYAVYQQMTSRYDHTSASILSLLLVVMAIIFLVTERWFRQRSRFYQTSGRYRQ
                     ATRHHAGPAGTALITGYVVLVFGAAFGIPAVMLIQWSIEAIAQGALDARFFGFIWNSS
                     FLSALAACGAVIIGLPLAYLASRRPSRLNLACLQAAYAGYALPGPVAALAVLVLFTHF
                     VPVLYGTVAVLLVAYILHFLPVGLQSMEPALQQVTPNVEEVARTLGCTTRRTLRRVTL
                     PLIRNGFIAAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPAALL
                     IVLLSLPALLLLVSKDWRGRGQEVVS"
     gene            9152..10273
                     /locus_tag="PK614_01520"
     CDS             9152..10273
                     /locus_tag="PK614_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB80923.1"
                     /translation="MPDAMDLAAEQSPVLELRGISCAYEPNRPAVEQITLTVQQGEIL
                     CLLGPSGCGKTTILRAIAGFERVTDGSISLSGRLVSSRDLMVPTEQRHIGMVFQEYAL
                     FPHLRVEKNIAFGLSHLSRPQQKAIVDDLLTLTGLRGLEQRYPHELSGGQQQRVALAR
                     ALALRPVLLLLDEPFSNLDPDMASRMRQDLHALLRQTKTTAILVTHDHDEAFSMADRV
                     AVLNRGRLEQFDTPEAIYHVPTTPFVADFVGQADFISGVVANDLVTTEIGEFPNTQHL
                     ATGTSVVVMIRPDDIHIVPTKGADAHILARQFKGSENVYTIQLPSGQIVHSSESSLSI
                     YQVGTAIALRVVATHTVLFPQPPESSGKKEPGHGNAPPP"
     gene            10360..10791
                     /gene="exbB"
                     /locus_tag="PK614_01525"
     CDS             10360..10791
                     /gene="exbB"
                     /locus_tag="PK614_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HRB80924.1"
                     /translation="MDLLKNAVEYGIIGMLIALSIWSVAVAVERWLYYRRVDLSQFTD
                     IQTFEMALTKRLVIIGTVAANAPYIGLLGTVLGIMMTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNILRRRVTELLTIYKVQHGTRG"
     gene            10775..11149
                     /locus_tag="PK614_01530"
     CDS             10775..11149
                     /locus_tag="PK614_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRB80925.1"
                     /translation="MEREVNQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EAGDHKDVPVVVTLTANGDLFLNDRPVPPDGLKTVMLAHPRESLVVVRADKVTLLERF
                     VSVVDEIRGLGFQSVSLEVVRL"
     gene            11713..12105
                     /locus_tag="PK614_01535"
     CDS             11713..12105
                     /locus_tag="PK614_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB80926.1"
                     /translation="MSGPTSQPPSELATSPAQTASLVPSSSTTQALRKPDYSWLAGPL
                     LQRIEALKQYPATARLHRLEGRVIVRIVIQQDGHITSATVARSSGHDVLDQAALETIR
                     QASPLTLSQPLEKSSVTMQIPLGYYLDR"
     gene            complement(12116..12487)
                     /locus_tag="PK614_01540"
     CDS             complement(12116..12487)
                     /locus_tag="PK614_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80927.1"
                     /translation="MMASSNSIQGGRSGWGLASGRRWEGMLAGIVVGLLVLSACSGSS
                     KYFHDAVNEATQEMVGKRYGSPHKTQAATDGGEIWTYFERGSGTAGFGGQVRGGSCRA
                     YVLTFDKQAVLRDWEQQPCQG"
     gene            complement(12471..13496)
                     /locus_tag="PK614_01545"
     CDS             complement(12471..13496)
                     /locus_tag="PK614_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRB80928.1"
                     /translation="MGYRFAVMSGIFLLLFSSGCATTRSESAPVVVKRTQMQMGTLVT
                     ITSVASDRRRAQDAASAGFQEIHRLEELLSTWIATSELSQVNAAAGRDAVMLSRDTMK
                     VLEASLEMARLTEGGFNILVGPAVEAWSVLDRQHIPSDAVLQAVRPLTELRSLHLNVA
                     ERTVYLAKPGMRVDVGGIGKGFAADMAVAAMQAAGATAGVVALSGDIRTFGRLPGGMK
                     FPFGVRHPRREEAVLAFIELQDEAISTAGDYERYFERDGVRYHHILDPITLQPARDCQ
                     SVTVVARDGLTADGLDTGIFVMGRERGLALVERLPGVGAVIVDRDGKVWVSSYLKGRV
                     RMNDGIE"
     gene            14147..15595
                     /locus_tag="PK614_01550"
     CDS             14147..15595
                     /locus_tag="PK614_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80929.1"
                     /translation="MKIRAILGALVLASLPAMPALAENMTPEDIKKLVDEAVEKRLQE
                     HERREGGMQQAQGEAASTPQYPVASGPITEIRVERKGEEKIPLGFGSTGSGKLIYAKP
                     FLSAPKATVGGYADVMYNSLSRQNLDNPSRNSFGQQRLVPFIYADITDHIKFATEIEI
                     ERGGSNAPQGTDGSMQIEFAQLDYLINEAINLRAGILLMPVGKFNLLHDSPLNDLVDR
                     PMVSRIVIPSTWFEAGAGIYGTLYPSSRSKLDYEVYAVNGMSQTAGGITDLGVRSARG
                     SVSRDRDDNKAVVGRIAFSPMLGIEIAGSGYHGAYKPAAGAVGTGYIDMFALDWTLQK
                     GPFEIIGESAWTRISNNNATGVANRVIGPAGMQGYYVQGNYHFMPEFLKKWAPSHFTD
                     ASTFTAVVRWEQVNTDTDDRTKQNALGGQRKLERLTLGLNFRPIEDTVIKFDWQFNTQ
                     RDAKGLVAAGDLGTAGSPMNGNGFLIQAATYF"
     gene            15656..16117
                     /locus_tag="PK614_01555"
     CDS             15656..16117
                     /locus_tag="PK614_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80930.1"
                     /translation="MTQHYRRLSVLLIPALLTACASLGGMQERFAVCSYDHAWEAALD
                     AVKDRAIARQDKAAGLIDTAWLEIPMPGRTFGALQRDLGDSKDRSRISLAVKRLDDVT
                     KIGFIEERQRWAFRGGSRLFGWTDTESSAQVMTDVQNRLDTKLKEHGCSVH"
     gene            16102..16788
                     /locus_tag="PK614_01560"
     CDS             16102..16788
                     /locus_tag="PK614_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRB80931.1"
                     /translation="MFSTLSAYRTLTAVVLLLTSSLPALATEKVWDNELRRFLKDDDF
                     KYEEFMTEEEAVKTILPKSQRVRKELIRLTQDRKELIEQRIGWKFPEDSFDLYIGETG
                     DKVDGYAMIHNTIGKYKPMTYMVGVDPKGNCTDVELLVFRDAKGSEVGKKRFNSQYDG
                     KTVTDPIRINKDIINISGATMSVRSMNAGVKRVLVLVDEFYLKPAGLGSDTVAVQKAE
                     RGLLGTLFGN"
     gene            16812..17330
                     /locus_tag="PK614_01565"
     CDS             16812..17330
                     /locus_tag="PK614_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80932.1"
                     /translation="MRNFNARFRLRDSDRHIRLLYTQFLFLMLVGFLFSFFWAHSMTS
                     LSPQGIADHYRGSDATFGEPMSFRELAEVTHFHLFTMPVVFMILVHVLYLTQASHATQ
                     VWLTWLSFGGVALDLLSPWLISYISPIFVLTMLAGDTLMMGSFLAMMAIPLYEMWILK
                     QPLMAGKRGEES"
     gene            17435..18190
                     /locus_tag="PK614_01570"
     CDS             17435..18190
                     /locus_tag="PK614_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRB80933.1"
                     /translation="MHPSISPINATTQLIQEITRQLDALDSLQLSKESLHAVRLLQLR
                     RLTRRIETLIPGHFGHGERTAHYARLLGKAISLTDDQHIELHYAALLHDIGLLTLPGR
                     LLDETAAHSLDDYALIQSHPREGAALLSPYRFLSEAARLIAHHHERWDGAGYPYGLRG
                     GYIPLAARILAIADVFDSIANRSTSLHRTLRTLQASAGSQFDPILTATFCARVRDQDQ
                     LPPLPFGDASATALTVEPTLDQPSHQHMPFSMF"
     gene            18253..18816
                     /locus_tag="PK614_01575"
     CDS             18253..18816
                     /locus_tag="PK614_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRB80934.1"
                     /translation="MMMNNAPDIAIPTDRLRTLSSELRAQSPHWADRLEQATTEQELR
                     NLVCDLFNLSHALPSHRESANETSTLAGRITRFINENLHRGLTLKVLANFLGYSEKYC
                     SDLFYRIMGESFSGYMRRHRVERAQSLLTTTAQTLAEVATAVGFSDQFAFSHFFKRAT
                     GYSPREIRSRQAHSRHRLTVHAMQKAL"
     gene            complement(19069..19548)
                     /gene="bfr"
                     /locus_tag="PK614_01580"
     CDS             complement(19069..19548)
                     /gene="bfr"
                     /locus_tag="PK614_01580"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRB80935.1"
                     /translation="MKAKEGVLDILNKVLVSELTAVHQYLLHAALCKHWGYGRLHEHF
                     NHLAQEEVSHSSGLIDHILYLGGAPEMDHLDAVAAGKKVSDLLEADLAFERDDADVLR
                     DGIAHCTKVADFTTRRRLEEMIVDTEEHIDWFETQLCTIRQVGLDRYLAEQIHSEKS"
     gene            complement(19584..20060)
                     /gene="bfr"
                     /locus_tag="PK614_01585"
     CDS             complement(19584..20060)
                     /gene="bfr"
                     /locus_tag="PK614_01585"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRB80936.1"
                     /translation="MKAKQGVIEILNKVLTADLTAINQYFVHAKMCSNWGYERLHHKV
                     RERSIDEMKDADELISHILYLEGVPNVQRMNTVHVGETVPEQLKLDLKAEQEMLTLLS
                     DGVVHCTKVGDFTTRHMFEDMAKDVDEHIDWIETQIETIKQVGLENYLAEQIHKES"
     gene            complement(20381..22720)
                     /gene="topA"
                     /locus_tag="PK614_01590"
     CDS             complement(20381..22720)
                     /gene="topA"
                     /locus_tag="PK614_01590"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRB80937.1"
                     /translation="MAKSLIIVESPTKARTITKYLGRGYSVMASVGHVKDLPTSKLGV
                     DLENDFEPQYVTIKGKSKVLADIKKKAQEVDTVFLAPDPDREGEAIAWHIAQELHGKG
                     KKKDGKVYRVLFNEITESAIKRALKSPGEIDMKLVQAQQARRVLDRIVGYQGSQLLWK
                     KVRRGLSMGRVQSVAVRLICDREKEREAFRAEEYWSIVALLAGDNPPAFEAKLHSVNG
                     QDADIGTGERAQQILDAVQGKPFVVQSIERKEKKRNPVAPFITSRLQQEAARKLHFSP
                     KKTMTLAQQLYEGIEIGAEGPTGLITYMRTDSPRISQEATEDARVVIRERFGVEYLPA
                     TPNVYKTQKAAQEAHEAIRPTSAARDPESIRQYLEQDQYNLYKLIWNRFIASQMVPAI
                     MDVTRVDSSPVGTPDRYVFRTTGTVVKFAGHTAVYLEGTDKELPSHKPKNEQEAEDES
                     DRQLPALREGESLHLVAQEAQTLPGLVSKQHFTQPPPRYNEALLIRELEEKGIGRPST
                     YASIISTIQDRKYVEKVEGRFLPTETGRTVNDFLLKGFPDLLDTEFTSHMEEQLDEVE
                     EGTKPWVSAVRDFYTPFVREMELAQGIPGPKDIVEPPTNLPCEKCGRMLEIKWGRNGK
                     FLACPAYKDDPPCKNTQNFEKLPDGTIKIVPKLEETTDEKCEKCSSPMVVKSGRFGKF
                     LACSAYPECKTTKAIALGVKCPQPGCGGDLVQKRTKKGRNFYSCSRYPQCEYALWDRP
                     INKPCPKCQAPFLIEKVSKQAGRSVQCRNEEECGYREAG"
     gene            complement(22803..23939)
                     /gene="dprA"
                     /locus_tag="PK614_01595"
     CDS             complement(22803..23939)
                     /gene="dprA"
                     /locus_tag="PK614_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRB80938.1"
                     /translation="MSHRSLRPWLVLRAIPGVGDAILLKLVHAFGAPDAVLSAPQSAL
                     EEIGCRPPLVEAIRRGPAPDAIRELDKELEQLQRRKVTVLTYLDAQYPAPLRTIPDPP
                     PLLYVQGSLLETDRHAVAIVGTRKVSAAGRIVAEELARDLAKMGFTIVSGLARGVDAA
                     AHRGALAGKGRTLAVMGCGLDRTYPADHRQLRETIEQEGAVLSELPLGAAPHSYHFPR
                     RNRIISGLSLGVVVTEAAIESGSLITARLAGEQGREVFAVPGFVKAEQSRGPNSLIKD
                     GARLVESAQDILEELLPQLDPAFRERLAGGAGTVAQPKVQFSPDETLVYDVLSVMPQS
                     VDDVIRRSGLPAAQVAAILLSLELNNCIRQLPGNEYARVTSGMH"
     gene            complement(24059..25570)
                     /locus_tag="PK614_01600"
     CDS             complement(24059..25570)
                     /locus_tag="PK614_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRB80939.1"
                     /translation="MGVEIAISIAREETRVAVLDNGVVTDLYGDRAKDQDFVGNIYKG
                     RVAKVLPGMQAAFIDIGLEKAAFMHVSDLSMDVEPGDTLVEGDDDDNKDSEVPKPKRQ
                     SSKPIEELLSEGQEVMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPTVEHIGVSRRIP
                     RDEERARLKEIMKRARRPGFGYIVRTVSEGVKEEELRSDVEFLHVLWQDVLTKRDQQP
                     APSLLHSDLSLSFRVVRDLFGRRVDRLLIDSREEYNAIKGFVQRFSPEQTSRIHFYDK
                     EESLFDYLGVEQEIARAMSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETI
                     LRNNLEAAREIAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMSSDKARTRIS
                     RVSDLGLIEISRERVREDLLRSLSEPCHYCDGRGYTKSPMSVAYEIFREVRRLGHEIE
                     QQRVVVGAHPAVALLLQEQEQPGVEELERRYSAKILVTPDDRLHLEQFDLVVM"
     gene            complement(25716..26834)
                     /gene="rodA"
                     /locus_tag="PK614_01605"
     CDS             complement(25716..26834)
                     /gene="rodA"
                     /locus_tag="PK614_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRB80940.1"
                     /translation="MIDRVIDSRGLDSFDLRFMGLIAVILSVGVLSIYSVTHSQDTAF
                     PFYLKQLVWILLGTIAFLVMYLSDYHKIARLAYPTYAVILIMLAVVLVMGKSSRGAQR
                     WIPIGPFAFQPSEFAKLVLVLVLANYYSRVSRAGWLHRVVLPGLIVLPGLLLILKQPD
                     LGSGLSFLAVYAAMLLMVGVRSKTLGVILLLSVMLFPFVWEMVWASLHDYQRERVMAF
                     VDPEYDPGGKGYHALQSRIAIGSGELSGKGLYGGTQSQLKFLPEGHTDFVFAVYAEEW
                     GFVGVLVLLALFIALIWVSLEIAARAKDTLGALLAAGIVAMLCFCVVVNIGMTAGMFP
                     IVGIPLPLVSYGGSATIMTMASLGLLLNVKRRRLTLFY"
BASE COUNT         5862 a         8488 c         7080 g         5475 t
ORIGIN      
        1 gagccatgca ggcatctagg ctcgctcgtt cactctagcc gattaccgga ggagtgtcga
       61 gactgctgct ttatgggagt gataagtacg tagataaacc gatgacaccg caaacacatg
      121 ctgtgcatgt ctgtgcggcg ggaagaagat ccctgcgaag catggacggc gcatcagccg
      181 gtttgcttga gtcggaccga ttgccaaccg accaaccaga cgccggcaag gatgagcgca
      241 atacccacga tctcgcggat gccgatcgcc tcacccaaca cgagcgcgga caagaggaga
      301 gccgataccg ggatgagatt tacgaacacg ccggcccgcg aaggacccac cccttgcacg
      361 ccgtacaacc aggcctgctg ccccaatgcc gtggcgaaca cgatgagata gcctaaggcc
      421 acccacccgg aaaccggcac cacactcgct ccggtcagca gcaacttctg atcggtccac
      481 agcagaggaa tttgcaacaa gagcgacagc agcagcgtcg tccagttgac ggtgagcgga
      541 gacagccgct ccatcacctg gcgaccaccg atggaataca gcgcccaact gaccaatccc
      601 aacaacacca gtgtgccgcc cagccaggga ttgtccccgc tcggcgcctc ccctccgaca
      661 ccggacacga gtgccactcc ggcaaaggac accgcacaac cggccgccac cgtgttgaat
      721 ggaatatctc gcacgagcag agacgacagt aaggccgtaa tggccgggct ggcgccgata
      781 atgacgccgg cggtcgcagc gccgatatac cgcaggccga agagcgtcag aagatgattg
      841 cccagcaccc cgagccccag cagcgacaac agcttgatat cgcgagcggt ccatgtcgta
      901 cgaccgccct cgctccacca ccagagagga atcaggatca ccagcgcgcc gaagccgcgc
      961 aacagggacg tctccactac cgaaaacgcc ccaagcgcca gcttctgtcc cacaatggac
     1021 ccgccccaca ccagcgccga ggtggtcaac gcggcatagg cggcgaatac cgatggtttc
     1081 tgcatggacg caattcctca atcgacgaca cacgtgggtt gccacgggcc gcgagcactg
     1141 gattctaggc ggatggtgtc ggggtgatga tccaaatacc gtctcggatg gtcgccaaca
     1201 cagcggacac ccgctggtct gtcctgacga cccggttgag ttcttgaatt gcagcagtac
     1261 ggtcgtcagg cggcggttcg agcaacacgt cgccgtccca caacacatta tcgatgagaa
     1321 tgaccccgcg cggggacaac aactccatcg cgcggcgata gtaattcacg tagttgagtt
     1381 tgtcggcgtc gataaaaatg agatcaaaag ggcccgtgag tcccgccatc gtctccaaag
     1441 ccggccccat acggatctcg acctttcgac catgagggga ttgcgcaaaa aaccgtcgag
     1501 caactgccgc ggacgcgtca tcgacttcgc acgtcgtgac ctgcccctgc tcgggcagcg
     1561 cttccgcgaa acacagcgcg ctgtagcccg tgaacatgcc gatctccagc actcgcctgg
     1621 cctgcaccag acgggccatc atctgcagga aggccccttc gagcggcccc accaacatac
     1681 gcgcaaactc catcgtccgt tccgtctcct cgcgaagcgc gcgccggacc ggagtttcgg
     1741 gcaatgaata ggcagccgcg taggcgtcaa ttttcggcaa cacgagatcg gtcatcggtt
     1801 tcgctccggc agagagttgt gcgacaattc gcaccggccg cgggatcata ccatggacgt
     1861 gcccggagaa ggagcctgaa ggtgaagaca ctggcgacat tggaaccctt gacgacacgt
     1921 ttgttggaaa tccgtcgcat ccagagcgcg gccgcagttc tctcgtggga ccaggagacg
     1981 tatatgcccg ccggcggcgg ggcggcgcgc gcggagcaga tcgcgacgct cgaaggtctt
     2041 gcccatcaaa gactcgtgtc cgctgagctc gaaacgctgc tcaccgaatg gatcgacccc
     2101 gcaaccggac aggccgccga cagttgggat gaaccctccc ggtcgctgct gcgggaaacc
     2161 tggcgcgact tcagccgggc caagaaactt ccatccgact ttgtgatccg cctcagccgc
     2221 gagtgttcct tggcccagca ggcctgggtg acggcacggg aagaaagccg gttctcaaaa
     2281 tttctgccgt cactgaagat catcctgggc ctcaaacgtg atgaggctcg gtatctgggg
     2341 tatcggaact cgccctacga tgcgctcttg gatacgtatg agccgggtgc caccatcgct
     2401 caactcgcgc cgctattcac acaattacgg gaacgccttg tcccgctctt acggcgcgtt
     2461 caggccagtc acgtcaccat cgacgaccgt tgcctgcacc agagtttcga ccagtccaag
     2521 caggtggagt ttggtcggct tgtgctgacc gccatgggct atgacttcga gcgcgggcgc
     2581 ctcgatctct cggcccatcc cttcacgacc tcttttcatc cgaccgatgt ccgggtaacg
     2641 acacgggtct tcgagaaaga tctgccgtcg tgcctgttta gttgcatcca tgaagggggc
     2701 catggcctgt acgatcaagg cctggaccct cgctattatg gctccccgct cggcgaatcc
     2761 gtgtcactcg gctttcacga aagccagtcc cgcctgtggg aaaattgtgt cggccggtcc
     2821 cgtgcgttct ggcactgctt ctacccgatg ttgcagcaca cgttcccgca acagttggcg
     2881 gacgtgcctc tcgaccggtt ctatgcggcc atcaaccggg ccaccccctc gctgatccgc
     2941 gtcgaagccg acgaattgac ctataacttg cacatcatgt tacgcgtcga aatcgagcag
     3001 gctcttattg agggacgagc agaaccggat gacctgcccg gcctctggaa cgagaaaatg
     3061 cagtcgtatt tgggtattgt gccggaacgg gatgccgagg gggtcctgca ggatgtgcat
     3121 tggtcgatgg gcgccttcgg atatttcccg acctacacct tgggcaacct gtattccgtg
     3181 caattcttcg aacaggccac actcgaactt ccgcagctgg aggaggagat gagagcaggg
     3241 cacctcttgc ctctgcggcg atggctcgag cagaaaatcc accgctgggg acggatgttc
     3301 acacccgacc atctggcacg acgggtgacg ggtagcgggg tgaaccccga gccgtttctc
     3361 cgttatctcg aaacgaaata cgcggagcta taccggctct gatctgtgag ccgcttttca
     3421 ctaccctccc atagcccact aacccctgcg tctccccacg acgccctttc tttcactcaa
     3481 ggattacgac aggtcaggca cacctctagg gccacaacag ggccctttag ctctattcga
     3541 ccaatcggtc ttcgtggtac tactccgccc cacatgaaat aggaaatttc tcttagtttc
     3601 ttaccctgct ccaggggatg aatcggctga gcacgtggga ttaggaatcg aaagacaagt
     3661 ccttggactt tcccgttggc ccgaatgggt tccggcagaa caaggtcttc agcgtcgatg
     3721 tccgagtcag tggctgccac gactagacga catgcaggag cctatcgagt gttctctccg
     3781 cgtacgaccc agacattgac cgccctccac gacgacaccg acttgagggc ggatcggacc
     3841 attctctgtg ctcggtcggg aacgggcaga gtggaattcc ggggtgctcc atgagcggaa
     3901 cgctggccgt ggatgcatgc caagactcgc acccggcgaa agccgaagtc cgaattctgc
     3961 tcgcggatga ccacgcctta ctccgtcgcg gcctgcgaga gttcctacgg gacttcctcg
     4021 ttgaggacgg actgacgccc gacattgtcg aaacagcctc tgcccgccat gcaatcgatc
     4081 acattcgcac aggcacctgg gatctcgtca tcctggactt gaatctgccg gacatgccgg
     4141 gactcgaagt cctgcgcatt ctcaaatcgt tgcggccggc cctgcctgtc ctggttgtca
     4201 gcatttatgc cgaagaacat tactcggctc gtgccattcg agccggtgca ctcggatacc
     4261 tgacaaaaga gcgagggccg caggaacttc ggatggcggt ctcacgcatc cttcagggtg
     4321 gacattacct gccgcctccc gaaaccgaac acaaccagga cgagggaccg caccggaacc
     4381 tctcctccac cgaccccctc ccggcaacac tctcggatcg cgaaattgaa gtgctgcagt
     4441 ggatcgcaca gggacgacgc ctgacggaga tcgcggaaca tctcaatttg agcatcaaga
     4501 cggtgagcac ctaccgttcg cggctcctca tcaaattagg aatgagaacc acggcagaat
     4561 tgattcgcta cgccctggac cagcagttgg tctgacactg aaggatgatc gagttgaccg
     4621 accctgcaca gcaattctct ccaagcggac gggcacctgt gatcggtctg cacagactcc
     4681 cacggcggcc ggtcagtaca ccctgcacct tctcatcgga ggactatggc caatattctg
     4741 atcatcaatg ccgatgcgaa tctgaagcgg cttatcaagc tggcgttacc gggcatggaa
     4801 cattctgttc aacacagtgc taccttacag accgcctgcg ctcagtccac gtcgtccaag
     4861 ctcgacctgg tgatcagtcg cttcgtcgcg gacgacgatc aagggccgaa agacttaatg
     4921 gaacttcgca aaacgttccc gaccgctcgc ctgatcgtca cagcgacgct gcacgatccc
     4981 gcactcgaag gcgagaaatt tcagcgcgtc gtccgcgaac tccacatcga gtattgtttg
     5041 ctggaaccga ttgagaccgg tgccatgctc catgcgatcc agtcggcgct cgcccttccg
     5101 aaacgaccct agatcgaccc gttttcaccg gtcgcccgcc acccaaccgc taccacaaca
     5161 cgtcagcgcc aacgacctac cggaccggga gcctccggtt cagtatccca tggccgtatt
     5221 gagcttcgcc aactggcttg gaggcaggag aaactccgcc gacgcttcga tgcgggcagg
     5281 aaccagcagc gtggtgtgga acacaatatc gcggatggga atcttgaaat cgggttgctg
     5341 cggggtactg agttcgactg attcgacgga ctgcgtaatg gcagtgacat cctgcggccc
     5401 gtaggtggtg atcaccgcct gcatgaggtc acgaattttc tcattggttt cgtttccgtc
     5461 gatgcctttc atcagcagag gaaggacctc gtcgcgaacc tggtcggacg ggctgaagtt
     5521 ttcgacccgt tccttgcgtc gaagggacgt caagtcattg tccagatcgc ggctgaaggc
     5581 gccataggca aaccggacga actcgaaatg aaaccccttc atcccctttc cgaacatctg
     5641 cagttcgcac aggaaacaga gctgctgcag cttgacgtca ctcaacaagc catagggctc
     5701 ggccagcgat aaggcataca gcagtagcac tcgatcgatc gtcatttcgt gagatttgcg
     5761 cacctattga atctcctctg aatcgtccat aaccggcagg actataaaca ggcaggtttc
     5821 ggttcgtcaa ttgatgatcg taccggccgg atcccctctt gacccgccga ggcactttcg
     5881 tctttaatgg gcgtctttca tgccggttca cgcgccatga gcaagactct caaagaaatg
     5941 gacgtgcttc gccagcacct ggccaagcat cagttgaagc tgactcgtca acgcgaactc
     6001 atcctgacgg cgttcctgcg acaagaacac gtcacggcag agacgatgta tcatcagctg
     6061 gcgaagaaag atccccacct cggactcgcc acgatttacc gcacgctgaa tctcttctgc
     6121 gaagcaggca tcgcccaagc ccgccacttc ggcacccaga cgcaatacga caatatctcg
     6181 cataagggcc atcacgacca tctcatctgc accggctgtg ggactattgt agagttcgag
     6241 aattgcgaga ttgaacggct acaggaagag gtcgcgacca agaacggctt tgtgatccaa
     6301 acccaccggc tggagctgta cggcctctgt gcgcgctgcc gccattgacg aatcgcttcc
     6361 gcatccagta ctatcttgag acggcttatc aatttcaact aggatggcca ttacaggctg
     6421 tccccactct ggaggcacgc cgcatggctt catcctgtcg cacatttttg gcgctgacgc
     6481 tactcctcgg agcgctgacg tttttgcagt gggccaccgg aaccgcggag gccgccgaga
     6541 aacttgtcgt ctattcgggt cgcgcagaac ggctcattaa gccggtgctg gatgaatttc
     6601 aggccaagag tggcatccaa atcgaattgc tgtcgtcggg cactaccgag ttggtcaacc
     6661 ggttacaagc cgaaggcgac cataccccgg ccgacgtctt cctgacgaac gatgccggga
     6721 gcctcgagca tgcccgggaa ctcaaactcc tccggcccat gaatatgcgc gaagttgaac
     6781 gagccatccc gtcgcaattc cgcgcggccg acaacagctg gatcggactc tcaggccgct
     6841 tctggatcgt cgtctacaac accaatctcg tcaaacccga tcagatcaaa tcactgttcg
     6901 acctcgccca gccgcaatgg aaagacaaaa tcgccgttcc caattccggt agcgaatatc
     6961 ttcaggccgg cgtttcagta atcaaagcca ccttcggaga cgaacggacg aagcagtttc
     7021 tgcaaggact caaggccaat gcgggcacgc aggtatacca aaagagttcg cagattgtgg
     7081 acgccgtcgc gaaggggcag gtcgcggcag gcattgtgaa ccactactat atctatcggc
     7141 accttgccac gcagcccacc gctccgatcg ccgcggtcat gaccgatcag caggaaggcg
     7201 gcatgggcgc catcatgaac gtgaccggaa tcggggtcac ccgcgcgtcc aaacacgttg
     7261 aaagtgccaa actcctgatc gaattcctgg ttgcccaggc cgggcaaaaa atgttcgcgg
     7321 atctggacaa ggaataccct ctccatccgg acgtgaaagc cgatccgaca ctcatcgacc
     7381 ggcgcacgtt ccgtgcggcg caagtccccc tggctcgact ggccgaatta cgcgaggcca
     7441 ccctcacgct gatcgaacag gtcggccttc gctaagccct tgtgtagccg cgcatgctta
     7501 ccgccacaag agctcacgcc ccctcttcgc tgcaatggat cagcctgatc actgccgcat
     7561 ttctggtgct ccccacctgc tacatcatct atgtggcgct caccgccgca ccgaccgtgt
     7621 ggagccgtct ctggtcgacg cgcattcccg aacttctctg gaacaccctc tcgctggcca
     7681 ccggcgtcgc tttgaccaca ctgctgctgg gcgtctccct cgcctggatc accgttcggt
     7741 acgaatttcc tggtcgccgg gtctgggaat gggcactcgc cttgcccctc gccatgccga
     7801 cctatgtgct ggcctatgtg tatgcccatc ttctgggaat gggcggcccg gtggaacaag
     7861 gatggcagac gctcatgggg tccgacgcgc gactgctctc ccctcaaagc tttgtcggcg
     7921 tcacactgat catggccctc gacacgtttc cgtttgtgta cctgctggtc cgaggagcac
     7981 tgctgaatct gaatatctcc ttcgaggaag tggcacgcgt ctgcggcgtt tctccttgga
     8041 gcaccctctg gcgcgtcacg ctcccgctca tccgccccgc cattgccgca ggactagcgc
     8101 tcgtgatttt gtatgtgatc tcggacttcg gggcggtctc tctgctccgc taccagacac
     8161 tcacgtatgc ggtctatcaa cagatgacca gccgctacga ccatacgtcc gccagcattc
     8221 tcagcctgct cctcgtcgtg atggcaatca tcttcctggt cacggaacga tggtttcgcc
     8281 agcggagtcg cttctaccaa acatccggcc gctatcgaca ggcgacccgg caccacgccg
     8341 gccccgctgg aacggcactc atcaccgggt acgtcgtact ggtgttcgga gccgcattcg
     8401 ggattccggc tgtcatgttg atccagtgga gtatcgaagc aattgcacag ggcgccctgg
     8461 atgcgcgttt tttcggcttc atctggaaca gcagcttcct gtcggcactg gcagcctgtg
     8521 gggccgtgat catcggactt ccgctggcct acctcgccag ccgccgtcct tcgcgactca
     8581 atcttgcctg cctccaagcc gcctatgccg gctatgcctt gcccggtcct gtcgccgccc
     8641 tcgcggtcct tgtgctcttt acgcacttcg tgccggtctt gtacggtacc gtcgcggtgt
     8701 tgctcgttgc ctacatcctg cattttctcc cggtcggcct gcagtcgatg gagccggctc
     8761 tccaacaagt cacccccaat gtggaagagg tggcacgcac tctgggctgt acgacccgcc
     8821 ggaccctccg gcgcgtgacc cttccgctca ttcgtaacgg ttttattgcg gcctgggtcc
     8881 tgatgttcct ccagaccatg aaggaacttc cggccacatt gctgctgcgg ccggtaggat
     8941 ttgatacact ggcgattcgt gtatggctcg aagccagcga agagtattat cagctggcgg
     9001 cgcccgccgc attgttgatc gtgctcctga gcctgccggc cttgctgcta ctggtatcca
     9061 aggattggcg cggacgtggc caagaggtcg ttagttgaat cccgtgaaca actcagacat
     9121 ccgcgagcac cgggcacagg gtgcatctac gatgcctgac gccatggatc tcgccgccga
     9181 acagtctcct gtccttgaac tacgcgggat ctcctgcgcc tacgagccca atcgtcctgc
     9241 tgtcgaacag atcacgctca cggtccagca gggcgaaatt ctctgtctcc tgggaccatc
     9301 cgggtgcgga aaaaccacca ttctccgtgc gattgcagga ttcgagcggg taaccgacgg
     9361 gagcatttct ctgtccggcc ggctcgtgtc atcgcgcgac ctgatggtgc cgactgaaca
     9421 gcggcacatc ggcatggtct ttcaagaata cgcgctgttt ccacatctgc gtgtggagaa
     9481 aaatatcgcc ttcggtctca gccacctctc ccgcccacag cagaaggcca tcgtcgatga
     9541 cctcctcacc ctcaccggct tgcgcggact ggaacagcgg tatccgcatg agctctcagg
     9601 gggacaacag caacgcgtcg cgctcgcccg cgccctcgcc ctgcgcccgg tgttgttgct
     9661 gctcgacgaa ccgttcagca atctcgaccc cgacatggcg agccgcatgc gacaagatct
     9721 ccacgccctg cttcgacaga ccaagaccac ggccatcctt gtcactcatg accacgacga
     9781 agcgttttct atggccgacc gtgtcgcggt cctgaaccgg gggagactgg agcaattcga
     9841 tacccctgaa gcgatttacc acgtccccac aactcccttt gtagccgact tcgtcggaca
     9901 ggcagatttt atctccgggg tcgtggccaa tgatctggtg accaccgaga tcggggaatt
     9961 ccccaacacg cagcatctcg cgactggaac cagcgtggtc gtgatgattc gccccgacga
    10021 cattcatatc gtcccgacca aaggcgcaga tgcccatatt ctggcaaggc aattcaaagg
    10081 ctccgaaaat gtgtacacga ttcagctccc ctccggacag atcgtccaca gcagcgaatc
    10141 gtctctgagt atttatcagg ttggcacggc gattgcgctt cgagtggtgg caacccatac
    10201 cgtcctcttc ccgcaaccac ccgaatcgtc aggaaagaag gaaccaggac acggcaacgc
    10261 cccacccccg taaaaccgga ctcagtccag gcctccatgc cgagagcagg gcgggggtgc
    10321 ccccttagac aaccaacacg agaggcggag gatcgacgaa tggatctgct gaagaatgcg
    10381 gtagaatatg gaatcattgg catgctgatc gcgctcagca tctggtcggt ggcggtggcg
    10441 gtcgagcggt ggctctacta tcgacgcgtg gacctgtccc aattcacgga cattcaaaca
    10501 tttgaaatgg cgctgaccaa acgcctggtc attataggca cggtggccgc caatgcccct
    10561 tacatcggat tgctggggac cgtcctcggt attatgatga ccttccatac gatgggaacc
    10621 tcaggcacga tggccgtgaa taccattatg atcggcttaa gcctggccct gaaggcaacg
    10681 gctgtcggcc tgctcgtcgc catcccctgc gtcgtgatga acaatattct ccggcggcgg
    10741 gtcaccgaac tgctgacgat ctataaggtg caacatggaa cgcgaggtta atcaaatcaa
    10801 cgtcattccg ctggtcgacg tgatgctcgt gctgctcgtc atcgtcctca ccaccgccac
    10861 tttcatcagc accggccaga tccctgtgaa tctcgccaag gccaaagagg ccggagatca
    10921 taaagatgtc cccgtcgtgg tgaccttaac cgcgaacggc gacctgttcc ttaacgaccg
    10981 ccccgtccca ccggacggtc tcaagactgt catgctcgcg cacccacgcg aatcgctggt
    11041 ggtcgtacga gccgacaaag tcacattact ggaacggttt gtctcagtgg tggacgaaat
    11101 acgaggtctt ggatttcagt ccgtcagtct ggaggtggta cggctgtgac gtcccgccag
    11161 cccggcatat cggacccctt catccgcttc catgcaagcg gatggatcat ctcactctgc
    11221 ctgcacggca gcgccattct cctggccgga ctcctcgtcg ccaaaatcgg cctggctcct
    11281 ccatcttcat cctttcaatg ggatgtcacg gtcgtcgggt ctcaacctcc ggcgacgcct
    11341 gcccaggtcg cacccagtac gcaaccacct gctacagcgg ttatccgacc ggctcaacgt
    11401 aacgctccag cggcggccct gcgtcccaca ccttctcagg cagcgctccc gaccataccg
    11461 gcaactacct caagctcttc ggcaacggtc gagcgcaaca ccatcgaagc agccctcacc
    11521 ccggctcacc aggctcccgc acaactgctt gaacgcgcgc ctgtggtgtc tcctcccgaa
    11581 cctcacgaaa cgaaggatga acttcaaact tccctgcaca cgacatcggc accggtcgag
    11641 gccccctctg atccccgctc actgccccag atggcgaatc cgccgcatgc agacgctcct
    11701 ctcaccccag aaatgtcagg accgacatca caaccgcctt ctgagctcgc cacctctccg
    11761 gctcaaacgg catcgctcgt tccctcgagc agtacgactc aggctttacg aaaacctgat
    11821 tatagctggc tcgcagggcc tttgctccag cggattgaag cactcaaaca atatcccgcc
    11881 acggcgaggc tgcatcgcct ggaaggtcgg gtgattgtac ggatcgtgat tcagcaagac
    11941 ggacacatta catcggccac cgtcgcaaga agctcagggc acgatgtgct cgaccaagcc
    12001 gcactggaaa ccattcggca ggcctcaccg ctcaccttgt cccagccgct cgaaaaatcc
    12061 tcggtcacga tgcaaatacc gctcggctac tacctggatc gataagccgc aggagctatc
    12121 cctggcaggg ttgctgttcc cagtctcgca agaccgcctg cttgtcgaac gtcaagacat
    12181 aggcccggca gctgcctccc cgcacttgcc caccgaagcc tgccgttccg ctgccgcgtt
    12241 cgaaataggt ccagatttct cccccgtcgg tagccgcttg tgtcttatgc ggtgatccat
    12301 atcgcttccc gaccatctct tgcgtcgcct cgttgaccgc atcgtgaaaa tatttggaac
    12361 taccgctgca tgcgctcaat acgagcaggc cgactactat ccctgcgagc ataccttccc
    12421 accgccgacc agaggccaag ccccatccgg aacgtccgcc ttggatggaa ttactcgatg
    12481 ccatcattca tcctcaccct ccccttgaga tacgaagaaa cccagacttt gccgtcgcga
    12541 tctacgatca ccgcaccgac ccccggcaac cgctcaacca aggccagtcc tcgctcacgt
    12601 cccatcacaa agatgccggt atccaggcca tctgcggtca gtccgtcgcg ggcgaccacg
    12661 gtcacgcttt ggcagtcacg agccggttgc aaagtgatcg gatcgagaat gtggtgatag
    12721 cgcactccat cccgctcgaa gtaccgctca tagtcaccgg ccgtcgaaat tgcctcatcc
    12781 tgaagctcaa taaaagccag cacagcctct tcacgcctcg gatgccggac accgaaggga
    12841 aatttcatgc cgcccggcag tctgccgaag gtcctgatat cccccgagag ggccacgacc
    12901 cctgcagtgg ctccggccgc ctgcatggct gcgacagcca tatccgcggc aaagcctttg
    12961 ccgatcccgc ccacatctac ccgcattccc ggcttggcca aatagaccgt cctctctgcg
    13021 acattcagat gtagcgaacg caactccgtc agcgggcgaa ccgcctgcaa gaccgcatcg
    13081 gatggaatgt gctgccgatc gagaacactc caggcctcaa ccgccgggcc gacgagaata
    13141 ttaaaccctc cttccgtcag ccgagccatt tcgagcgagg cctccagcac cttcatcgtg
    13201 tctcggctga gcatgacggc gtccctgcca gcggcagcat tcacctgcga gagttcgctg
    13261 gtcgcaatcc aggtactgag caactcttcc aagcgatgaa tctcctggaa tccagccgat
    13321 gccgcatcct gcgcccgccg acgatcagac gccaccgacg taatggtcac gagcgtaccc
    13381 atctgcatct gcgttcgctt cacgacgaca ggagcggatt cagaccttgt agtcgcgcat
    13441 ccggacgaga acaagagcag gaagatcccg ctcatgactg caaaacgata tcccatctcc
    13501 acctcaccac ctccgacgaa tatgacaaag agactgcgct taacactcaa tagatcacac
    13561 aacttcattg gcgcagcccg gcgctgattt acgcgcacac gcgtccatct tctgcgggac
    13621 gcctccaaaa tgcaaatgtc gcccgtcgcg ccgaccggca ggcaaacact ccgttcccct
    13681 accccactca tccgacaaaa tcacctgtgg caggcgcgca gcgcacacaa aaaagcggcc
    13741 aacttttaat tggtcgccag acttgacatt cctccgctga tcgggatatt aataatcgtt
    13801 ctcaatttca ccaacgagac cgaccacccg ctatgatcac aggaaaaagt ttggcatccc
    13861 cttcctgcca agacagtgag acgcgctgcg agtgcggaca actcattgcc aaagtccgtg
    13921 ggcaaggcct tgagctcaaa tgcaaacgct gtaagcgcat cgtcgtcatc ccattcacct
    13981 caatcgaagg ctggggcacc gtcacagcat gattcgatat atgaaaggag gcctcgtcac
    14041 cggcagaact ccaggggctc aaccacacac acatgctttc ttcgcagacc agagaccttt
    14101 gagtcccgag tccaaccaaa accttactcg cggatggagg atacccatga agatccgggc
    14161 aatcctcggg gctctcgtct tggccagctt accggcgatg ccggccttgg ccgaaaacat
    14221 gaccccggag gatatcaaga agctagttga cgaagcagtt gagaagcggc tgcaggaaca
    14281 cgaacgacgt gaaggcggca tgcaacaagc ccagggtgaa gcggcctcga ctccgcaata
    14341 tcctgtggca tccggtccca taaccgaaat tcgagtcgag cgaaagggcg aggaaaagat
    14401 tccgttggga ttcggatcaa ccggatccgg caaactcatt tacgccaagc ccttcttgag
    14461 tgctccgaaa gcaacggtcg gtggatatgc cgatgtcatg tacaactcac tgtcccgtca
    14521 gaacctggac aaccctagtc gtaattcatt cggccagcaa cggctggtgc catttattta
    14581 tgcggacatt accgaccata tcaagtttgc aacggaaatc gagatcgagc gcggcggctc
    14641 caacgcgcca cagggcactg acggttccat gcagatcgag tttgcccaat tggattacct
    14701 gattaatgaa gcgatcaatc tccgagctgg tatcttgttg atgccggtcg gcaagtttaa
    14761 cttactgcac gactcaccct tgaacgatct cgtcgatcgg ccgatggtct ctcgcatcgt
    14821 catccccagc acctggttcg aagccggtgc cggtatttac ggaaccctgt atccctcgtc
    14881 acgctcgaag ctggactacg aagtctatgc cgtcaatgga atgagccaga cggctggcgg
    14941 cattaccgac cttggggtgc gaagcgcgcg aggcagcgtc tctcgggatc gcgacgacaa
    15001 caaagccgtc gtcggccgca tcgccttcag cccgatgctg ggcattgaaa tcgccggatc
    15061 cggctaccat ggagcgtaca agccggccgc cggagccgtg gggacgggct atatcgatat
    15121 gttcgcattg gactggactc tccagaaagg cccgttcgaa atcatcggag agtcagcctg
    15181 gacgagaatc agcaacaaca atgcgactgg tgtcgccaac cgggtcatcg gaccggctgg
    15241 catgcaaggc tactacgttc aggggaatta tcacttcatg ccggaattcc tcaagaaatg
    15301 ggcgcccagc catttcaccg acgcctctac gtttacggca gtcgtgcggt gggaacaggt
    15361 caacacggat accgacgatc gcaccaagca gaacgcgctg ggaggccaac ggaagcttga
    15421 gcgcttgacc ctcggtctca atttccggcc gattgaagac acggtgatca aattcgactg
    15481 gcaattcaat acccagaggg acgcaaaggg ccttgtagcc gccggggatc tcggcactgc
    15541 aggcagcccg atgaatggaa acggattctt aatccaagca gccacgtact tctaaccggt
    15601 tcctcgtcca ggccgtcccg atcgggacgg cctggacgat accgacagaa ttgatatgac
    15661 acagcactac cgccgactca gcgtcctcct gattcccgct ctcctgaccg cctgcgcatc
    15721 gctgggcggt atgcaggaac gattcgccgt ctgcagctac gaccatgcct gggaagctgc
    15781 acttgacgca gtcaaagatc gtgcgatagc gcggcaggat aaggccgcag gcctgatcga
    15841 caccgcgtgg cttgaaatac ccatgccggg acggaccttt ggtgcccttc agcgagactt
    15901 gggggatagc aaagatcggt cccggatttc cctggcagtc aaacgactcg acgatgtcac
    15961 aaaaatcggc ttcatcgaag agcgacaacg gtgggcattc cgtggcggct ctcgcctgtt
    16021 cggctggacc gacaccgagt cctcggccca agtcatgacc gacgtgcaga accgcttaga
    16081 caccaaattg aaggagcatg gatgttcagt acattgagtg cgtaccgcac cttgaccgca
    16141 gtcgtattgc ttctgacctc gtcgctcccg gcgctggcga cggagaaagt ctgggacaac
    16201 gaattgcgcc gcttcctgaa agacgacgac ttcaagtacg aagagttcat gaccgaagag
    16261 gaggccgtga agaccatcct cccaaaatct caacgtgtgc gcaaggaact gatccgactc
    16321 acgcaggaca ggaaggaact catcgaacag cgcatcggtt ggaagtttcc ggaagactcg
    16381 ttcgatctgt atatcgggga gactggagac aaagtcgacg ggtatgcgat gatccacaac
    16441 accatcggca aatataaacc catgacctat atggtaggag tggatcccaa agggaattgc
    16501 accgacgtcg aattgctggt gtttcgggat gcaaagggca gcgaggtcgg gaagaagcga
    16561 ttcaactctc agtatgatgg caagaccgtc acggacccca ttcgaatcaa caaagacatt
    16621 atcaacatca gtggagccac gatgtccgtc cggtcgatga acgccggcgt caaacgagtg
    16681 ctggtactgg tagacgaatt ttacttgaag ccggccggct taggcagcga cacggtcgca
    16741 gtgcagaagg ccgagagagg actgttgggg accctattcg gcaactaagg ccgtcgggac
    16801 actgcactgg catgcgaaat tttaatgctc gattccgcct gcgtgattcg gatcgtcata
    16861 tcaggttgtt gtatacgcaa ttcctctttt tgatgctggt ggggtttctt ttttccttct
    16921 tctgggcgca cagcatgacc agcttgagcc cacaaggtat cgccgaccac taccgtggct
    16981 ctgatgcgac cttcggcgaa ccaatgtcgt ttcgggagct ggccgaggtc acgcactttc
    17041 acctgttcac catgccggtc gtgtttatga ttctcgtcca tgtcctctat ctcacgcagg
    17101 ccagtcatgc cacgcaagtc tggctgacat ggctttcgtt cggaggcgtg gcgctggacc
    17161 tgctctcccc ctggctgatc agctacatct cacctatttt tgtgctcaca atgctggccg
    17221 gggacactct gatgatgggc agctttctcg cgatgatggc tatccctctc tacgaaatgt
    17281 ggattttgaa gcaaccgctg atggccggga agcgagggga agagtcatga acgcctagaa
    17341 gaagtccgtt gactccgttg cggccagagg tattcagccc agagcccaag atcgtgtgca
    17401 acgatcttgg gctctttttt ttcggaggat tcccatgcat ccatcgatat caccgatcaa
    17461 tgccacaaca cagctcatcc aggaaataac ccgacagctt gatgccttgg attcacttca
    17521 gctttccaaa gaatcattgc acgctgttcg cctgttacaa cttcgacgcc tgacgcggag
    17581 gatcgaaacc ctcattcctg gacacttcgg acatggcgag cgcaccgcac actatgcccg
    17641 cttgctgggc aaagccatca gccttacgga tgaccagcac atcgagctgc actatgcagc
    17701 cctgctgcac gatatcggac tcctcacgtt acccggcaga ctgctcgacg agacagcagc
    17761 acacagcttg gatgactatg ccctgatcca aagccatccc cgggaaggcg ctgcgctcct
    17821 gagcccctat cgattcctct ctgaggccgc acgcctgatc gctcatcatc acgaacgatg
    17881 ggacggcgcc ggatatcctt atggacttcg cggaggctat atccccttgg ccgccagaat
    17941 tttggccatt gcggacgtct tcgacagcat tgccaatcga tcgacttcgt tacacaggac
    18001 cctacgaacc ctacaagcgt cggcaggctc acaatttgat ccgatactga ccgccacatt
    18061 ttgtgcacgg gttcgcgatc aagaccagct cccgccgcta ccattcggtg acgcgagtgc
    18121 caccgctctc accgttgaac cgactctcga ccaaccgagt caccagcata tgcccttttc
    18181 aatgttctga ggagtcgaac gaaaacagtc catacccggc gtacgggcta ttggcctcac
    18241 tgatggagct gaatgatgat gaacaacgcg cccgacatcg caattccaac tgatcgcctc
    18301 cgcaccctct cgtccgagct tcgcgcacag tcaccacact gggctgatcg tcttgagcag
    18361 gccacgacgg aacaggagct acgcaatctc gtgtgcgatc tctttaacct gtcccatgcc
    18421 ctgccgtccc acagagagtc cgctaatgag acaagcacgc tggccggacg catcacccgc
    18481 ttcatcaacg aaaatctcca tcgtggactg acactcaaag tcctggcaaa tttcctcggc
    18541 tattcggaga aatattgctc tgatctcttt taccgcatca tgggtgaatc gttttccggg
    18601 tacatgagac gacatcgggt cgaacgggct caatcactcc tcaccacaac cgcgcagacg
    18661 ttggcagaag ttgctacagc ggtggggttc agcgatcagt ttgccttcag ccatttcttc
    18721 aaacgcgcga ccgggtattc tcccagggag attcgatccc ggcaggcaca ctcacggcat
    18781 cggctgacgg ttcacgccat gcagaaagca ctgtagccaa cggccagacc aaccacacgg
    18841 cttcactgct tggagaacgg gaatgtcccc tgccctgaat ggccatggaa tacagaacga
    18901 tcgagtcgct tcgcagtagt gaggggagaa tgaatgcgcg ggtgattcaa attaccggac
    18961 ggaggggttc agcgaagcat gtgcgtgggg agagacgacg acagaggaga gagccggcca
    19021 gcggggcctt cggggatccc accggccgac cacctgcgca cgcaagggct aagacttctc
    19081 agaatgaatc tgctcggcca agtatcgatc gagacccact tgcctgatgg tgcagagctg
    19141 agtctcaaac caatcgatat gctcttccgt atcgacgatc atctcttcga gccgacgacg
    19201 ggtcgtgaag tcagcgacct ttgtgcaatg ggcaatcccg tcgcggagga cgtccgcgtc
    19261 gtcacgctcg aaggccagat cggcctccag cagatccgac actttcttgc cggccgcaac
    19321 cgcatccaaa tgatccatct ccggcgcgcc acccaggtac agaatatgat caatcaaacc
    19381 cgaggaatga ctcacttcct cctgagccag gtgattgaaa tgttcgtgca gccggccgta
    19441 gccccaatgt ttgcacaagg ccgcatgcag aagatattgg tgcaccgccg tgagctcact
    19501 caccagcact ttgttcaaaa tatcgagaac gccctctttc gctttcatgc atactcccat
    19561 tcccacgccg ggccggcgct cgcttaactc tctttgtgaa tctgctcagc caggtaattc
    19621 tcaagcccca cttgcttgat cgtctcaatt tgggtctcga tccagtcgat gtgctcatcc
    19681 acatccttcg ccatgtcttc aaacatgtgg cgcgtggtga aatctcccac cttcgtacaa
    19741 tgcaccaccc cgtcgctcag aagggtgagc atttcctgtt cggccttgag atcaagcttc
    19801 aactgctcgg gaacagtttc cccgacatgc accgtgttca tacgctgaac attcggcacc
    19861 ccttccaagt acagaatgtg actgatcagt tcgtccgcat ctttcatttc atcgatgcta
    19921 cgttctctaa ccttgtgatg cagccgctca taaccccagt tcgagcacat tttcgcatgg
    19981 acgaaatatt gattgatcgc ggtgagatcc gcggtgagga ctttatttag aatctcaatg
    20041 accccttgtt tggctttcat aggtccctcc ctccagcctt ggagtgcaat gctttccgcc
    20101 ggcatgttgg tccacgccga cgcgggcgtg cgatcgaccg actctttact ctacacctta
    20161 attagagatt ctcgaagcgc ttgtcaacta gagaagtacg tagaaatgag gcgagttatc
    20221 aacatcgccg agaacgcttc tcattcttct catcaggaca agatcgggaa cggcgtgtcc
    20281 cgcaagagct cggcggggac acgaactgga gtcgaggggg gagatgcaga acaactgatc
    20341 gaagcgagcg aaccgcggca gaggaacgga tgcgccttga ctatcctgct tcccgatagc
    20401 cgcactcttc ttcattgcgg cattgcactg aacggccggc ctgcttactg actttctcaa
    20461 taagaaacgg cgcctgacat ttgggacagg gcttattgat ggggcggtcc cagagcgcat
    20521 actcacattg cgggtaacga ctgcacgaat aaaaatttcg cccctttttc gtgcgcttct
    20581 gaaccagatc acccccgcac ccgggctgag gacatttcac acccagagca atggctttgg
    20641 tcgtcttaca ttctggatac gcggaacagg ccaggaactt accgaagcgg cccgatttca
    20701 ccaccatcgg gctggagcac ttttcgcact tctcgtccgt cgtctcttcc agcttgggaa
    20761 caatcttaat cgttccatcc ggcaactttt caaaattttg cgtatttttg cagggaggat
    20821 cgtccttata ggcggggcac gcgaggaact tcccattccg tccccatttg atctccaaca
    20881 tgcgaccgca tttttcacaa ggaaggttcg tgggcggctc gactatatcc ttcggtccag
    20941 ggataccttg agcgagttcc atttccctca caaagggtgt gtaaaaatcg cgcaccgctg
    21001 aaacccaggg ctttgtgccc tcttcgactt catcgagctg ctcttccatg tgggatgtaa
    21061 attccgtatc cagcagatcc ggaaatccct tcaacagaaa gtcattcacc gttctcccgg
    21121 tttcggtcgg gagaaaccgc ccttcgacct tctcgacata cttccggtct tggatcgtcg
    21181 aaatgatcga agcgtaggtg gagggccggc cgatcccctt ttcttccagc tctcgaatca
    21241 gcaatgcttc attgtaccgg ggaggcggct gagtgaaatg ctgtttagaa acgagcccgg
    21301 gcaaggtctg ggcttcctga gcgaccagat gcaaggattc accttcgcgc aatgcgggca
    21361 gctggcgatc tgattcgtcc tccgcctctt gctcattctt gggcttgtgc gacggcaatt
    21421 ccttgtcggt tccctctaag tacaccgccg tatggccggc gaacttgacc acggtgccgg
    21481 tcgtgcgaaa cacatatcga tccggagttc ccaccggaga cgaatccaca cgcgtcacat
    21541 ccatgatcgc agggaccatc tgtgaggcaa tgaagcgatt ccaaatcagc ttatacaggt
    21601 tgtactggtc ctgctctagg tattgcctga tggattcggg atcgcgcgcc gcggacgtcg
    21661 gccgaatggc ttcatgcgcc tcttgcgcgg ctttctgggt tttatacacg ttgggcgtag
    21721 caggcagata ctccactccg aaccgctccc gaatcacaac tctggcgtct tcggtcgctt
    21781 cttgcgaaat cctcggcgag tccgttctca tgtaggtaat gagaccggtg ggcccttctg
    21841 ccccgatttc aatgccttca tacaactgct gcgccagcgt catcgtcttc ttcgggctga
    21901 aatgcagctt tctggcggct tcctgctgaa gccggctcgt gatgaagggt gcgaccggat
    21961 tgcgtttctt ttccttgcgc tcgatcgatt gcacgacgaa cggcttcccc tgcacggcat
    22021 ccaggatctg ctgggcccgc tcgcccgtgc cgatatcggc atcctgaccg ttcacggaat
    22081 gcaatttggc ttcgaacgct ggtggattgt ccccagccaa caacgcaacg atagaccagt
    22141 attcttccgc ccggaaagct tcgcgctcct tctcccggtc gcaaatcaac cgcacggcca
    22201 cggattgcac ccggcccatg ctgagaccac ggcgcacctt tttccacagc aactgactgc
    22261 cctggtaccc cacgatgcga tcgaggactc gtctcgcctg ctgcgcctgg accagcttca
    22321 tatcgatttc acccggcgac ttcagtgccc gcttgatcgc cgattccgtg atctcattga
    22381 acagcacacg atagactttg ccgtctttct tctttccctt gccgtgcagt tcctgcgcaa
    22441 tatgccaggc aatcgcctcg ccctcgcgat cagggtccgg cgcgagaaac acggtgtcga
    22501 cttcctgggc tttcttttta atgtcggcaa ggacttttga cttgcccttg atcgtgacgt
    22561 actggggttc aaaatcgttt tcgagatcaa cgcccagctt gctggtgggg aggtctttga
    22621 catggccgac ggaggccatg acggagtagc cacgaccgag atatttcgtg atggtccgcg
    22681 ccttggtcgg cgactcaacg atgatcagcg atttggccat gcggctccac gttcctcagt
    22741 acctacgccc agactaccac acccactata acaaatatcg agcccttgtc agctctaatc
    22801 tctcaatgca tgcccgatgt gacacgcgcg tactcgttgc cgggtagctg acgaatacaa
    22861 ttgttcaatt cgagcgataa caggatcgcc gccacttgag cggcaggcaa tccactgcgc
    22921 cgaatcacat cgtcgaccga ttgcggcatg acggacaaga catcgtatac caacgtttca
    22981 tccgggctga attgcacctt cggttgggcg acggtccctg ctccccctgc cagccgctca
    23041 cggaaggccg gatcaagctg cggcaacaat tcctccagaa tatcctgcgc cgactcaacc
    23101 aagcgggcac catccttgat caaactgttc gggccacgac tctgttcagc tttgacaaaa
    23161 cccggcaccg caaacacttc ccgcccctgc tcaccggcca atcttgccgt aataagagac
    23221 ccgctctcaa tggccgcttc cgtcactact acgcccaacg acaatccgct gatgatgcgg
    23281 ttgcgccgcg ggaagtgata gctgtgcggt gccgccccca acgggagttc agacaacacg
    23341 gctccctcct gttcgatcgt ctcccgcaac tgccgatggt cagccggata ggtccgatcc
    23401 agtccgcagc ccatgacggc aagcgttcgc cccttaccgg caagggcccc gcgatgcgcg
    23461 gccgcatcga ctccacgcgc caaaccactg acgatcgtaa accccatctt cgccaagtcg
    23521 cgcgccaact cctcagccac gatgcgcccg gcggcactga cttttctcgt gccgacaatc
    23581 gccacggcat gccgatcggt ttccagcagc gagccctgaa cgtacaacaa cgggggcggg
    23641 tccggaatcg tcctgagcgg cgcggggtac tgtgcatcaa gatacgtcag caccgtaacc
    23701 ttccggcgct gcagctgctc taattccttg tcgagttccc ggatcgcatc cggagccggt
    23761 ccgcgacgaa tggcctcgac cagagggggc cggcatccga tctcctccaa ggccgactgc
    23821 ggcgccgaga gcacagcatc cggcgccccg aatgcatgca caagcttgag aagaatggcg
    23881 tcgcccacac cgggaatcgc gcgcaacacc aaccagggac ggagagatcg atgactcact
    23941 atcgtctcaa ctcatcacac gtcgtcagtg cgatgtcatg cccgttgtac tggagaaaga
    24001 ccgcgttcag cggcaggatg cccacccaga acaggtacac gcccggtcgc tcggcggatt
    24061 acatcacgac cagatcgaat tgctccagat gcaaccgatc gtcgggagtg accagaattt
    24121 ttgcgctata tcgccgttcg agttcctcga cgcccggttg ctcctgttcc tgcaagagca
    24181 gagcaacggc aggatgcgcg ccgacaacca cgcgctgctg ctcgatctca tggccaagcc
    24241 gccgcacttc acggaaaatt tcgtaggcca ccgacatcgg cgacttggtg tagccgcgac
    24301 cgtcgcaata gtggcagggc tccgacaacg aacgcagcaa gtcctctcgc acacgctcac
    24361 gggaaatttc gatcaacccg agatcggaca ccctggaaat tctggtgcgg gctttatccg
    24421 acgacatggc gtccaccagc gcatggtaga ccttgtcgcg atttttctcc cgctccatgt
    24481 cgatgaaatc cacgatgatg atgccgccga tcccgcgcaa cttcacctga taggcaattt
    24541 cgcgcgccgc ttccaggttg tttctgagga tcgtttcttc ctgatcgcgc ttcccgacaa
    24601 accggccggt attcacgtcg atcaccgtca tagcctcggt atggtcgatg accaaatacc
    24661 ctccggactt gagccacacc ttgcggctca tcgcacgggc aatttcctgc tccacgccca
    24721 ggtaatcgaa caggctttct tctttgtcgt agaaatgaat ccgcgaggtt tgctccggcg
    24781 aaaaccgttg cacgaacccc ttgatcgcgt tgtattcctc gcgggaatcg atcaataatc
    24841 ggtccacccg acgaccgaag agatcccgca cgacgcggaa gctcagactc aaatcgctgt
    24901 gcaacagact cggagccggc tgctgatcac gcttcgtcaa tacgtcctgc cataagacat
    24961 gcaggaactc gacgtccgag cgcagctcct cttccttgac cccttcactg actgtccgca
    25021 cgatgtagcc gaaccccggg cggcgcgccc gcttcatgat ttctttcaat cgcgcccgct
    25081 cctcatcacg agggatacga cgggagacgc cgatatgctc gaccgtcggc ataaacacga
    25141 ggtaccggcc cggcaaggac acataggtcg tcacgcgcga tcccttggtg ccgatcggac
    25201 ctttggagat ctgcaccatg acttcctgtc cctcggacag caactcttca atgggttttg
    25261 aactttgccg tttgggcttg gggacctccg agtccttgtt atcgtcgtcg tcgccctcta
    25321 cgagggtatc tccgggttcg acatccatcg agaggtcgga gacgtgcatg aatgccgctt
    25381 tttccaaccc gatatcgatg aacgccgcct gcatgccggg cagcactttc gccacacggc
    25441 ccttataaat attcccgacg aaatcctggt ctttggcgcg gtcgccatag aggtcggtga
    25501 caaccccgtt gtcgagtacc gccacgcggg tttcttcccg cgctatactg attgctatct
    25561 ccacacccat agaaactcct ttaccctcag atcgggcgga tcggcagacg aacgcggacg
    25621 cacagcccgg gttcttcctc gtctcgccgt cagtccggca ccaatctggt tcacatggtt
    25681 atttcatacc cagtcggccc ctgtccaggc atggcctaat agaacaaggt caaccgacgt
    25741 cgtttgacgt tcaacaataa tcccaatgag gccatggtca tgatcgtggc gctccctccg
    25801 taactgacca acggaagcgg aatgccgaca atgggaaaca tccctgcggt catcccaatg
    25861 ttcacgacca cacaaaaaca gagcatcgcc acaatccctg ccgcgagtaa cgcccccaag
    25921 gtatccttcg cacgcgcggc aatttccaat gagacccata tcagcgcaat aaacagcgcc
    25981 aagagcacca gcactccaac aaagccccat tcttccgcgt acaccgcaaa gacgaagtcg
    26041 gtatgccctt cgggaaggaa cttcagctgg ctttgtgtgc cgccgtacag cccttttccc
    26101 gacaactccc cggagccgat ggcaattctg gactggagcg catgatagcc ttttcctcct
    26161 ggatcgtact ctggatcgac aaaggccatg acacgttccc gctgataatc gtgcagggat
    26221 gcccagacca tttcccacac gaacgggaag agcatgacgg agagcaacaa aatcaccccc
    26281 agcgtcttcg agcgcacgcc gaccatcaat aacatggccg cgtacacagc cagaaaactc
    26341 agaccgctcc ccagatcagg ctgcttgagg atcaacagca ggccgggcaa cacaatcaac
    26401 cccggcagaa ccacccggtg cagccacccc gcgcgagaca cccgggagta atagttcgcc
    26461 agcaccaaca ccaggaccag tttggcaaat tcagacggtt gaaacgcgaa gggcccgatc
    26521 ggaatccacc gttgggcacc acggctggac ttacccatga ccaacacgac cgccaacata
    26581 atcagaatca ccgcataagt cggataggcc aggcgtgcaa tcttgtgata gtcggacagg
    26641 tacatcacca ggaacgcgat cgtgcccagc agaatccaca cgagctgttt gagataaaaa
    26701 ggaaaggcgg tgtcctgaga atgcgtgacg ctgtagatcg acagcacccc gaccgacagg
    26761 atgaccgcga tcaaccccat gaaacggagg tcgaaactat ctaaccctcg actgtcgatt
    26821 acccgatcaa tcatcacgtc tttggtcgcg cgtcagcgtt ggtccgacga tcgaatggtg
    26881 acggaggggg cgttgtaatc gcgtg
//
LOCUS       DAORQN010000010        26778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_125_length_26778_cov_210.780451, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000010 DAORQN010000000
VERSION     DAORQN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26778
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_26778_cov_210.780451"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK614_01610"
     CDS             <1..207
                     /locus_tag="PK614_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRB80941.1"
                     /translation="YMYLSEIYRISANLAGLPGIGMPCGFSKAGLPIGFQLIGRPFEE
                     ETLLRGAHAYEQATNWRAKRPAIR"
     gene            226..627
                     /locus_tag="PK614_01615"
     CDS             226..627
                     /locus_tag="PK614_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HRB80942.1"
                     /translation="MIVDVAAILVAIAFAVLVGYLVPLLIQIRKMVAESEQLVTKLNV
                     ELPTLITELRAMSQNLNDVTEQARGGVEHAAVLLHAVGEIGESVNQVHSVVRGSGGSL
                     LANVASVVAGFRAAKQVVTERFKEGGHHNGG"
     gene            617..937
                     /locus_tag="PK614_01620"
     CDS             617..937
                     /locus_tag="PK614_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRB80943.1"
                     /translation="MADNQGPSSAAVLLGFLSGAALGAVTAILLAPRTGQESREMLRG
                     YARRAEDGLRDLVGEAGERFEGAVEEGRDFIESKKTVLRDAFDAGREAMRREREHFTK
                     GEQS"
     gene            940..>1302
                     /locus_tag="PK614_01625"
     CDS             940..>1302
                     /locus_tag="PK614_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRB80944.1"
                     /translation="MAYEVVIGVEVHAQLRTQSKLFCACGTSFGLTANSQTCPVCLGL
                     PGTLPVINEKAVEMAVRAGLAMNGTIGVQNRFARKNYFYPDLPKGYQISQYEAPICEH
                     GWIEIAAGGSRKRVRIRRA"
     assembly_gap    1303..1332
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1333..2382
                     /gene="gatB"
                     /locus_tag="PK614_01630"
     CDS             <1333..2382
                     /gene="gatB"
                     /locus_tag="PK614_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRB80945.1"
                     /translation="GKNLHEVGSGMSLVDLNRAGTPLLEIVTEPDLSSSEEVVAYLKA
                     LRDLLMYLDVCDGNMEEGSFRCEPNLSLRPVGQTTFGTKVELKNINSFKFVKDAVDFE
                     IKRQTKVLNEGGKIYQETRLWNHERGETAVMRSKEEAHDYRYFPDPDLVPMEISSEWI
                     EQLREGLPELASTKQQRFITEYGVPEYDAGILTSSKALAMYFDACVKLYPQPKTVSNW
                     VMGELLRELNNSGTEADASPVTPQRLVELLTLVDQGVISLKVARDMFPDVYASGKAPA
                     QIVQEKGLTQVSDEGALATMIDEVLKKNPAQVGQFKEGKQQVLGFLVGQVMKASGGKA
                     NPGKVNELLKKQLQG"
     gene            2501..2851
                     /locus_tag="PK614_01635"
     CDS             2501..2851
                     /locus_tag="PK614_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80946.1"
                     /translation="MKLSQRSDEEILEVAGPIMDNLMEASTDIDHERHVRDFTERLKS
                     LVTKDYLEKVCRQYQTENGFFSKRECVAIFRRPQSVAIVWKQWFSKQQGEFVAEMVLV
                     EQDSRHLVDHVMVF"
     gene            2926..4212
                     /gene="eno"
                     /locus_tag="PK614_01640"
     CDS             2926..4212
                     /gene="eno"
                     /locus_tag="PK614_01640"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRB80947.1"
                     /translation="MSGIRNVKARQIIDSRGNPTVEVEVLLESGAHGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVANVSKTIAPRLMGMEALDQAAVDHEMIALDGTKT
                     KGKLGANAILGVSLAVAKAAANETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIMPVGAPRFSDALRMATEVFHTLKSLLKKKGLNTAVGDEGGFAPDLQSNEEA
                     LALIMEAIEAAGYRPGQDIALALDCAASELYEKGRYRLEAEKNPERSSEEMVAYYGKL
                     LDRYPILSIEDGLSELDWKGWKILTEKLGKRVQLVGDDIFVTNVEIFAKGIAEGIGNS
                     ILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEDELGAAAVYRGRAAVPSRA"
     gene            4218..4580
                     /locus_tag="PK614_01645"
     CDS             4218..4580
                     /locus_tag="PK614_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRB80948.1"
                     /translation="MMIKPNRGRDWLDWQRKLCSAGKWVGLGALFLMMGTLLFGEMGI
                     SRYLHLRDHAEQLDQELADLQRLNGELRTDLDRVQYDPTRIEELARERLGYVRKGETV
                     YQLAPIGEKERVPTVRTP"
     gene            4577..5461
                     /gene="era"
                     /locus_tag="PK614_01650"
     CDS             4577..5461
                     /gene="era"
                     /locus_tag="PK614_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRB80949.1"
                     /translation="MKFGSVAIIGRSNVGKSTLLNRLLKEKIAIVSDKPQTTRTRILG
                     VAHVEGAQIVFLDTPGFHEPHHLLNRRMVRTTLDTFDDADVLYVVVEATAQPGPGDLA
                     VIEHVKQAVAKQARPVVLVINKVDLVNKSRLLPLMEQYLRIFPWTEIVPVSAETADNV
                     DRLLSVTVSLLAEGEATFGDDVVTDQSMRTLAAELIREKILQQTYEEVPHSVAVEIEE
                     FIETKKLTKIGAVVLVEKESQKGILIGKHGERLKLVGTAAREDMERLFGMKVFLKVWV
                     KVRESWREDEQTLAELGY"
     gene            5565..6902
                     /gene="mgtE"
                     /locus_tag="PK614_01655"
     CDS             5565..6902
                     /gene="mgtE"
                     /locus_tag="PK614_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRB80950.1"
                     /translation="MRLVSIQRLLRRGAITNLAKMLARMHPADIANVIDHLSSLKEKR
                     EIFELVRGDVKRGQVLSELDGESITLVLSDLLPSDAAWLLKDLGSDDIAYILSVIPND
                     RAKEILALMRTEDSTEIADLLKYPKGTAGGIMTTEFFALFEDATAQEAIRRLQQATDA
                     EMVFYIYVTDKEDRLVGVLSLRQLLTVPPATPLKNIMTRDLISVAVDMDQEEVSRQVA
                     SYNLLAIPVVEKDGKLVGIITVDDVVDVIREEATEDMLKMAGAIEEDAMFKSSSMAAA
                     RVRLPWLFTNLVGSLLSGMLLWMFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIR
                     GLATGLVELTDVWKIFFREAKIAFLMGIACSLMLTVVGWLWHQVFLGMVVGVSLITAF
                     LVSTSMATVMPIVLKRMGVDPAVAAGPFVTTANDITGIAIYLTLATIFLEQIR"
     gene            6912..7715
                     /gene="recO"
                     /locus_tag="PK614_01660"
     CDS             6912..7715
                     /gene="recO"
                     /locus_tag="PK614_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRB80951.1"
                     /translation="MGLLVSLSSFTLGGEMPLVKTAAIVLHSRKWGDADRIVTFYTMR
                     IGKLRGVARGARRLKSRLGGMIEPFTLCQLDLFEKPGDSLYRISQVALDEPFAKFRDD
                     LTLMTAAGRMVNLVSAVMAEGDPEPRVFEMLESGLRTLLESRDAAWTTLLFQIRLLGM
                     TGFRPQTEQCATCGQVHRSSLPQFSPVAGGIVCERCASRQPFRCTALSRGSVAFLHQA
                     LHMQPALLNRLHAAGQVRTEVESAIESYVTVVAGRRLPPVDFLTSGSPA"
     gene            7779..8135
                     /locus_tag="PK614_01665"
     CDS             7779..8135
                     /locus_tag="PK614_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRB80952.1"
                     /translation="MSETVLEPTDMEWVAKGVLSITWSDGHKAFYPVRYLRQHCPCAA
                     CTDEWTGELRLKPDDVPMVIMLQDVQPVGRYAFQFTWSDGHDTGIYSYTFLRRMCQCD
                     VCQPVKPVEPRSRRLL"
     gene            8181..8642
                     /locus_tag="PK614_01670"
     CDS             8181..8642
                     /locus_tag="PK614_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80953.1"
                     /translation="MTQARIIAVGWWWLVAILSVSCSSLPSLDQQKQLVQQGDYRIHQ
                     LTPRAFVETWGEPTYTHQQFTHFFGMQDGQLIPQSRMALGESPQGWETGLAAGDALFL
                     AYADRGQYLVFLDEALVYHEAMTPEKVHAVGKTWKYESQFKTRLELSPAMK"
     gene            8703..10193
                     /gene="glgA"
                     /locus_tag="PK614_01675"
     CDS             8703..10193
                     /gene="glgA"
                     /locus_tag="PK614_01675"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRB80954.1"
                     /translation="MVSSEVVPFAKTGGLADVVGALATEFARLGHDVCVLLPAYRQVD
                     ALGYQMVDYARLAVPTAQGLVEARIQELTGPHSHVTGSGRLRVLTVRHDAFFSRSGLY
                     QEAGHDYPDNLERFAFFCRAVMELLVHFGEKDGWQADLLHVHDWQAALCAVYLRTLYQ
                     AKSALSNIRSVLTIHNLGYQGLFPAEHFSLTGLPAHLFTPAALEFYGKLNLLKGGLVF
                     ADLLTTVSPTYSGEIQTPEYGCGLEGVISGRKDVLHGIVNGIDAEIWNPAKDPHLPTQ
                     YSLSDMSGKARSKKGLQRELKLRTDKGPLVGVIARLTGQKGIDLVIDIIPELMELDVQ
                     VVILGTGDVKYEQLVRELAERYPGRLAVRNVFDEGLAHRIEAGADMFLMPSRYEPCGL
                     SQLYSLRYGTVPIVRKTGGLADTVVNYTPSAFKGSRVTGFSFTDTSADSLLTCLLLAL
                     SIYRKKADWQRIVRAGMEQDLSWARSAEIYLRLFQELLEGKEPQRK"
     gene            complement(10219..10716)
                     /locus_tag="PK614_01680"
     CDS             complement(10219..10716)
                     /locus_tag="PK614_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80955.1"
                     /translation="MSDRRDIRVGILAILVAALSVVGCQSNPAITSVRYDNVRFMDVW
                     STYTHCLASNQSQSALLDSHKLREVSQAQALRSPLENFLPTKLKSMVTQPASRLAVDV
                     HAMAAACSLHAGNLAVSVGEHDLARNEFRQVLENQTQSDYSYYASQARERLSYLDLTL
                     QAALR"
     gene            11527..13161
                     /gene="aepX"
                     /locus_tag="PK614_01685"
     CDS             11527..13161
                     /gene="aepX"
                     /locus_tag="PK614_01685"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HRB80956.1"
                     /translation="MNASQALTKTRQFRNLLLSEQLQFICEAHNGLSAKIVQEAGFPG
                     IWASGLSISAQFGVRDNNEASWTQVLEDLEFMSDATRIPILLDGDTGYGNFNNMQRLI
                     RKLEQRNIAAVCIEDKLFPKTNSFLKGDAQPLADMQEFCGKIKAGKDAQTDPDFCLIA
                     RVEAFICGWGLAEALRRAEAYHQAGADGILIHSALSVPDEILAFKREWGNRCPVVIVP
                     TKYYATPTDVFRQHGFSMVIWANHMLRAAVASMQKTARALKESEHLLSIEDKVAPVSE
                     IFRLQNAAELLEAEERYLPRGAEGTSAVVLAASRGEELGELTEDQPKTMVKVQGVPIL
                     SHIVDAYNAVGIKDILVVRGYKKEAVNLPNLTYIDNLEFAETGELASLSQALQSRRGR
                     FQPTIVSYGDVLFNKYIPQALCQEQDDCVIFVDSNWRDQSSYARLGGFAECSIPNSRK
                     AFNAKIHLKQLGKTLPEESIHGVWMGFLKLSSSAASQVNDLLLDMLADPANRRAGIPQ
                     LLQALLKQDVPVRVLYTVGHWLDVNSLEDVVQAGNF"
     gene            13279..14406
                     /gene="aepY"
                     /locus_tag="PK614_01690"
     CDS             13279..14406
                     /gene="aepY"
                     /locus_tag="PK614_01690"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HRB80957.1"
                     /translation="MIDPQDFIKHLQANGVEFFTGVPDSLLKEFCACLEHLPRAGHHI
                     IGANEGGAVALALGYHLATRKVPLVYLQNSGLGNIINPLLSLADPEVYSVPLLLVIGW
                     RGEPGVHDEPQHKKQGRVMLSMLEAMEIPHSILGPELVDPQAALADALAHVRKMSAPY
                     ALVIKKGTFKPFAVPKVEKTEFPLSREEAIQQVIESLGERDVLISTTGMPSREVYEYR
                     SKRGEGHQRDFLTVGGMGHASQIALGIALQKPGRPVYCLDGDGALLMHMGALAITGAL
                     KPRNFKHIIVNNGAHDSVGGQPTVAFDVDVPGIAHASGYESVFCAQTKQELQSRLAEL
                     QRSSGPSLLEVRVRCGARKDIGRPVTTPSQNKNAFMDFVEN"
     gene            complement(14545..15156)
                     /locus_tag="PK614_01695"
     CDS             complement(14545..15156)
                     /locus_tag="PK614_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB80958.1"
                     /translation="MAQNAVHSQEPADNLADQRAGAGIVVLSSSMQLLHMNRQAAELS
                     KLINMAENGGAPAKSAQGVLPAALTELCTEVLKALQVRSEAKDWEQFEVKRIAGNPNQ
                     PVLLRGFGLPDRGGIQYARLVVTLEELGRRQQLNTDQAKERFQLTNREQSVVENLAKG
                     WTNKEIANALKITEQTVKEHIKHIMRKTNSTTRTGVLVQVFRS"
     gene            15984..16685
                     /locus_tag="PK614_01700"
     CDS             15984..16685
                     /locus_tag="PK614_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRB80959.1"
                     /translation="MNGKALLSGIILVGNVIGVAHAGQFDQVPKPTLVAGSSPGASSS
                     GVRHGGEGAEKSSLTVTPDYIMGPEDVLEITVWKNADLSKQVQVRPDGRISLPLIGDV
                     SAVGRTAAQLTEEISARLKSYMENPTVSILVREVNSYQIYVLGEVNAPGKYPLKSKTT
                     LLQAITIAHGFTQVAARNKIVVFRFGKDGEGLNKIKASYDDIVLRDGSDQNIELKPGD
                     QIVVPSETMVVLPSR"
     gene            16747..17370
                     /locus_tag="PK614_01705"
     CDS             16747..17370
                     /locus_tag="PK614_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRB80960.1"
                     /translation="MKTFGCRWLRGGVCIVFSLLLPLPGCWIGNEQIDFNVTYIPPNE
                     FLLGPEDVLVVNIWRNQELSREVIIRPDGKISMPLIGDVQAAGMTSNVLAKRIADGLA
                     EFMSNPTVSVQVKEVNSYYVYVLGEVSKPGKVPLKSYATVLQGISLAGGFTTFASRNK
                     IHVLRVVPNGQGQPKQVQIPVPYEDIIQGKNSEGNFFLKAGDVIVVP"
     gene            17294..18961
                     /locus_tag="PK614_01710"
     CDS             17294..18961
                     /locus_tag="PK614_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80961.1"
                     /translation="MKTLFKVKTRREISSSRRVMSLLCRDQCLKIGWLCYGVFALVLC
                     WTISDSAAQQIRVVPSISVIEQYDSNVFFTPKSQLPAGTKVDDLITIVTPQLNFIQGN
                     TLVKSNLSVGAVVQKFINNSELDNVGFNASAGIDLSQAVNRVLPRMRGFRVFGTYQYM
                     PSTPAFGAGGLGGGFGGGGGFGGGFGTGGIGIGGPIDSGLLTQRIRTTMYNAGFSDSY
                     SLSPTTDFQTSYTYSQLSFGGALTPTTTTSGQPLNTVFDTTTHSITAGPTSRISAVDT
                     LTVRYTFTQMSQAQFGDYATHGGTLGWGRSWTKEWSSSVNGGLTLIEPIPDTSVAGGQ
                     RRIPATMFPTGGFSVSYASGSSFLRKLGSEIQEATGTSGAGATGGAGFLPLLAGMNMP
                     GGIAAPGSYRVTLMYNLGVFPSFVQSAGPIYTHMVSLGSMAGITNKLSAQAMFNYARS
                     TYTSDAIGTTFSSYGTTVTLNYLITPMFTARLSHQWLMFDAETSGFNAADFGFAKQMI
                     LLGFTYAYSPRGDFFRSGAFWEGSSGASSSAESGVSKSGSGGVDTKK"
     gene            18958..21312
                     /locus_tag="PK614_01715"
     CDS             18958..21312
                     /locus_tag="PK614_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRB80962.1"
                     /translation="MNTRTLSPEDYWRAVVSRKWFVISAILVSLSIAGVVCALTPKIY
                     QSATKMWFEGAKIEESIVSGPTPAGSGYSPTLDDRVMEVRQFVMGRKTLGQIAGEFGL
                     FGYEKDHPDAPESENAIRAMRGSIKVEPTKDKLFITLSFSNEDPIIARDVTSRLSDLF
                     IEETLKDRERGVEAAEDFLGLELKHAKAELEVKEKIISEFKQQHLGELPQQIDANLRK
                     LDRLQDDMRSQSEQAQNLANRLAQIDKSIKDYEETGEISSDSPVGGSSKRNKDPRLGR
                     IKELERRLVELSSIYKETYPDLVQVKEEIKKLREMTSAQYRDLLPDSEGVEEPAVGKK
                     SKRKAIDPYHAELLKQREEIVLELDAVKRHQAHISSEISQYERRVEHTPEREQKLKTL
                     ERDYENLQKNYQSLLDKKLSAGMQKSLAQGRKGAKFSIVDPAYTPVVPVVPNIPLIMA
                     AGLVLGCALGFGGAVGLELMGRGFRSAEEVELTLGLPVIASIPLYESAFGGSMQTVRA
                     LSGKSRNAAVLLPRYGKQGESLEISGGLPVASVSKSRGANGKLNDPTPGLELVSMWRP
                     LSFVAEQYRVAATRLELMTGDRKSTAIVVTSAVMGEGKSSTALNLGYVLAKDLDRRTI
                     VIDCDLKRPMQHIYAGVWQQPGLAEVLRGTKVVEDCLQRLGEAGPWILTAGAVGDNPL
                     ALSKMHQLADLIAELKEKFDYVIIDAPPVLPLADMQVLASMGDLLAYVVKASMTGRDV
                     VQKALRVIGDTANVGIILNGLDAHTTPYYMQQEYYREAHHEQLK"
     gene            21296..22363
                     /locus_tag="PK614_01720"
     CDS             21296..22363
                     /locus_tag="PK614_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRB80963.1"
                     /translation="MNSSSKSEREIGLEPVVTQPPLVSVSVSFPKFTRRVLILGVGPL
                     ARDLCQTLLSKRTGFTEVVGFLDKDASRVGERLVNPSIIGTYDQLFEIAERYQVHTVA
                     VCLEDRRAVLPVQTLLDMKAMGRDVVDGHYLYEEESGRLSIDHLKPSALIFSTGFRRR
                     LVTMLLKRCLDVLVAIVGIVALVPLVLLLSILIKLDSCGPVFYRQMRVGLRGRPYMIW
                     KFRSMRQDAEQSGARWATSEDPRVSRVGRWIRKWRLDELPQLINVMRGEMSLVGPRPE
                     RPVFVQELRNTIPYYDLRHTVRPGITGWAQTRFRYGASKEDSHVKLQYDLFYVKNLSL
                     ALDLRIVIHTVKVMLMGEGAR"
     gene            22373..23260
                     /locus_tag="PK614_01725"
     CDS             22373..23260
                     /locus_tag="PK614_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRB80964.1"
                     /translation="MPDAIPRHCLSFDVEEHFQVSAFETPMRRRHWGQFESRVEANTE
                     RLLGLLENRGVRATFFILGWVAERYPSLVRRIASGGHEVASHGYAHELITSQTPSTFR
                     EDIRKAKGILENILSQPMLGYRAPSFSITKDTMWATQILVEEGYVYDSSIFPVLHDRY
                     GVPSANPEAHQLLTASGLLWEVPPSTVKCLGVRLPVAGGGYFRLYPYALLRALLRKLE
                     AEGASLVMYMHPWEFDPDQPRMEGSLVSRMRHYLNLDKTEIRMRELLQDFSFAPIREV
                     FPQIEQMHSARLVSQVSGV"
     gene            23842..24660
                     /locus_tag="PK614_01730"
     CDS             23842..24660
                     /locus_tag="PK614_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB80965.1"
                     /translation="MAVLVIEPRTGYVQVGWGELWAARELLYFLAWRDLKTRYAQTAI
                     GAGWALMQPLLSTLIFTLVFSYLAKVPSDGLPYPLFAFAALLPWSLFARSLERSTLSV
                     VTEGGLIKKVYFPRLIIPIAATFINLVDFTVGLLILIGMMTWYQVLPQWTVLFLPLFV
                     IVALLTALSVSLWLSALNVKYRDVASVVPLMTQLWMFASPVLYPASLVPESLRWYYGL
                     NPMAGVIEGFRWALLGKSAPDWTMVAVSLGVVSFLLIGGVMFFRRVERTFADLI"
     gene            24661..25932
                     /locus_tag="PK614_01735"
     CDS             24661..25932
                     /locus_tag="PK614_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB80966.1"
                     /translation="MSNTAVSVTGLSKRYRLGTTHAQDGSLAGALTRGLRHLIGGQPA
                     SPQTHDTLWALRDVSFEIKKGEVFGVIGTNGSGKSTLLKILSRVTEPTNGRALIHGRF
                     CGLLEVGTGFHPELTGRDNVFMSGAILGMKRQEIARKFDEIVAFAEVEQFIDTPVKHY
                     SSGMYVRLGFSVLAHMDPDILIVDEVLAVGDVRFQKKCMGKMEDVGQHGRTVILVSHD
                     MPAITRMCSRAILLNKGEIVQAGPAHEVVNHYLHAGHIQPAVEWLDPIQAPGNDVARL
                     RALRVRSEAGQVADVIDIRKPMRVEIEYDILKPGQMVVAQFGFNTEEDVIAFIVNDRD
                     PVWYRRPRPVGRYVSTAWIPGNFLSEGTMVVGGGIFSEDPWHEHCDVPRAVGFRVVDP
                     GTGDTARGDVTGRWEGVVRPLLRWTTEHSSL"
     gene            26562..>26778
                     /locus_tag="PK614_01740"
     CDS             26562..>26778
                     /locus_tag="PK614_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB80967.1"
                     /translation="MKAVILAGGLGTRLSEETVLRPKPMVEIGGKPILWHIMQIHAVY
                     GVTEFIIALGYKGEMIKEYFLNFFAINN"
BASE COUNT         5842 a         6307 c         8181 g         6418 t
ORIGIN      
        1 tatatgtatt tgtcggagat ctacaggatc tcggcgaatc tggcgggatt gcctggcatt
       61 gggatgcctt gtggattcag caaggcggga ttgccgatcg gctttcagtt gatcggccgg
      121 ccgtttgaag aagagaccct gttgcgtggg gcgcatgcct acgagcaggc aacgaactgg
      181 cgggcaaaac ggccggcgat tcggtgagcc tgacaaggag gccctatgat tgttgatgtc
      241 gccgcgattc tggtcgccat tgcctttgcg gtgctcgtgg gctacctcgt cccgctcttg
      301 attcaaatac gcaagatggt ggccgaatcg gagcagctgg tgacgaagtt gaacgtcgag
      361 ttgccgacgc tgattaccga attgcgcgcg atgagtcaga atctgaatga tgtgaccgag
      421 caggcgcgtg gcggggttga gcatgcggcg gtcctgctgc atgccgtagg ggagatcggt
      481 gaatcggtca atcaggtaca tagtgtggtg cggggctccg gcggttcgtt gttggccaat
      541 gtggccagtg tagtggcggg gtttcgcgcg gcaaagcaag tggtgacgga acgtttcaaa
      601 gagggagggc atcacaatgg cggataatca aggtccatcg tcggcagcgg tattgttggg
      661 cttcctgagc ggagcggcgt tgggtgcggt aacagccatc ttgttggcgc cgcgaaccgg
      721 acaggaatca cgcgagatgc tgcgcggcta tgcccggcgc gcggaggacg ggctgcggga
      781 tttggtcggc gaagcgggtg agcggtttga aggggcggtc gaagaaggtc gcgacttcat
      841 tgaatcgaag aaaaccgtgt tgcgcgatgc gttcgatgca gggcgggaag cgatgcgtcg
      901 ggagcgcgag catttcacga agggggagca gagctgagcg tggcatacga agtcgttatc
      961 ggcgtggaag tgcatgcgca gctgcgcacg cagtcgaaat tgttttgtgc ctgcggcacg
     1021 agcttcggcc tcacggcgaa ttcgcagacc tgtccggtct gtttggggtt gcccgggaca
     1081 ttgccggtca tcaacgagaa ggccgtggag atggcggtgc gcgccgggtt ggcgatgaac
     1141 ggcacgatcg gggtgcagaa tcgcttcgcg cgcaaaaact atttttaccc ggacctgccg
     1201 aaggggtatc agatttcgca atacgaagcg ccgatttgtg aacacggctg gattgaaatt
     1261 gcggccggcg gcagccgcaa gcgtgtgcgc atccggcgcg cgnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnggaaagaa tctgcacgag gtcggcagcg ggatgagcct cgtggatctg
     1381 aatcgcgccg gaacgcccct gttggaaatt gtgaccgaac cggacttgag ctcttctgag
     1441 gaggtggtgg cgtacctcaa ggcgctgcgc gacctcttga tgtatctgga tgtctgtgac
     1501 gggaacatgg aagaggggag tttccgttgc gagccgaatc tgtccctgcg gcccgttggg
     1561 cagaccacgt tcgggacgaa ggttgagttg aagaacatca actcgttcaa gttcgtgaag
     1621 gacgcggttg atttcgaaat caagcggcaa accaaggtgc tcaacgaggg cgggaagatt
     1681 tatcaggaaa ccaggctctg gaatcacgag cgtggagaaa ccgctgtgat gcgcagcaag
     1741 gaagaggcgc atgactatcg ttattttcca gaccctgact tggtcccgat ggagatttcg
     1801 tccgaatgga tcgaacaact gcgcgaggga ctgccggaac tggcctcgac caagcaacag
     1861 cgatttatca cggagtacgg cgtgcccgag tatgatgcgg ggattctgac ttccagcaaa
     1921 gcgctggcga tgtatttcga cgcctgcgtg aaactgtacc cgcaacccaa aacggtgagt
     1981 aattgggtga tgggtgaatt gttgcgtgaa ttgaataatt ccgggactga ggcggatgct
     2041 tcgccggtga cgcctcaacg gctggtcgag ttgctgaccc ttgtggatca aggggtgatc
     2101 agtctcaagg tggctcgaga catgttccct gacgtctatg catcgggaaa ggcaccggcc
     2161 caaatcgttc aggaaaaggg gctgacgcag gtctccgatg aaggggcctt ggcaacgatg
     2221 attgatgaag tcttgaagaa aaatcctgcc caggtcgggc aatttaagga aggcaagcag
     2281 caggtgcttg ggttcctcgt cggacaggtg atgaaggcct cgggcggcaa ggcgaatccc
     2341 gggaaagtga atgagctgct gaagaagcaa ctgcaagggt gaggctaggt tgcttccctt
     2401 gctcgtgcaa cgcgtggcct gggaaggcca tcgttggacg cgcgcagtgg aaaacgaccc
     2461 ggcctcatcc tgaaagcatc agacgagggc agggtgttgc atgaaactct cacaacgatc
     2521 cgatgaagaa atcctcgagg ttgcaggccc gatcatggac aacctgatgg aggcatcgac
     2581 cgacatcgat catgagcggc atgtgagaga ttttacggag cgattaaagt ctcttgtcac
     2641 gaaggactat ctggagaagg tgtgcaggca gtaccaaacc gaaaatggat tcttttcgaa
     2701 gagggaatgt gtcgccatat tccggcggcc ccagtctgta gcaatagtat ggaaacaatg
     2761 gttttcgaag cagcaaggcg agttcgtggc tgaaatggta ctggtcgaac aggactcgcg
     2821 tcatttagtc gatcatgtca tggtcttcta gcggtttcga tcacaaaacg attcggtaga
     2881 agtccggaaa tggtttagcg tcagtcagta gaggagaatt tgagcatgag cggaatcaga
     2941 aacgtgaagg cgcggcagat cattgattca cggggcaatc ccacggtgga agtcgaagtg
     3001 ctcctggaga gcggtgcgca cggtcgtgcg gcggtgccgt caggcgcctc gaccggtgaa
     3061 aaggaagcca tcgaattgcg tgacggcgac aagaagcgct ggatggggaa gggtgtctcc
     3121 aaggcggttg cgaatgtgag taagaccatc gcgccaaggc tgatgggcat ggaggcgttg
     3181 gatcaggcgg ccgtcgatca cgaaatgatc gcgctggatg gaacgaagac caaggggaaa
     3241 ttgggcgcca atgccattct gggcgtgtct ctggcggtgg ccaaggccgc ggccaacgag
     3301 accggtcagc cgctctatcg ctatcttggt ggaacgaacg cgcgggtgtt gccggtgccg
     3361 ctcatgaaca ttatcaacgg cggcgctcat gcggataacc ggctcgatct gcaggagttt
     3421 atgatcatgc cggtgggtgc gccccgcttc agtgatgcgt tgcggatggc cacggaagtg
     3481 tttcatacgc tgaagtcgct cctgaagaaa aaagggctca acacggctgt cggcgacgag
     3541 gggggattcg cgcctgattt gcaatcgaat gaagaggccc tcgcgctgat tatggaagcg
     3601 atcgaagccg ccgggtatcg ccccggtcag gatatcgcgc tggctttaga ctgcgcggcc
     3661 agcgaactgt atgaaaaagg gcggtatcgg ctggaggctg aaaagaatcc tgagcgttca
     3721 tccgaggaaa tggtggccta ttacggcaag ctgttggatc gttatccgat cctttcgatc
     3781 gaagacggct tgagcgaact ggattggaag gggtggaaga ttctgacgga aaagctcggc
     3841 aagcgagtgc aactcgtggg cgacgacatt ttcgtcacga acgtcgagat ctttgccaag
     3901 gggattgcag agggcatcgg taattcgatc ctgatcaagc tcaaccagat cggcacgttg
     3961 acggagacgc tggatgccat tgaactcgcc aagcggtccg ggtatacggc catcatctcc
     4021 catcgctcgg gggaaacgga agatacgacg atcgcggatg tcgcggtggc gacgaacagc
     4081 ggattgatca agaccgggtc gttgtccagg acggatcgag ttgcaaaata caatcaactg
     4141 cttcgcattg aggatgaatt gggcgcggcg gccgtctatc gaggccgtgc agcggtccca
     4201 tcgagggcct aatcgtgatg atgatcaagc cgaaccgggg tcgcgactgg ttggattggc
     4261 aacggaagct ctgctctgcc ggaaagtggg tggggctggg agcgctgttc ctcatgatgg
     4321 ggaccctgtt gttcggcgag atggggattt cgcggtactt gcatttgcgt gaccatgcgg
     4381 agcagcttga tcaggagctg gccgatcttc agcggttgaa cggtgaactg cgtacggatc
     4441 tggatcgtgt gcaatacgat ccgactcgca tcgaagagct ggcccgggaa cggttaggat
     4501 atgtgcggaa aggagaaacg gtgtatcaat tggctccgat tggagagaag gaacgggttc
     4561 ccacggtgag gacaccatga agtttggaag tgtggccatc atcggacggt cgaatgtcgg
     4621 gaaatcgacg ctcctcaatc gtctgctgaa agaaaaaatc gccattgtct ccgacaagcc
     4681 gcagaccacc aggacgcgga ttctgggtgt ggcgcatgtt gagggcgctc aaattgtgtt
     4741 tctcgatacg cccgggtttc acgagccgca tcacttgttg aatcgccgca tggtgcgcac
     4801 gacactggac accttcgacg atgccgatgt gctgtatgtg gtggtcgaag ccaccgctca
     4861 gccaggccct ggcgatctcg ctgtcatcga acatgtgaag caggcggttg ccaagcaggc
     4921 gcggccggtg gtgctagtga tcaataaagt cgatctggtg aacaagtcgc gattgttgcc
     4981 attgatggag cagtatcttc ggatttttcc ctggacggaa atcgttccgg tctcggctga
     5041 aaccgcggac aatgtggatc gcctgttgtc ggtgacggtg tcgctcctgg cggaaggtga
     5101 ggcgacgttc ggcgacgatg tggtcacgga tcaatcgatg cgcaccctcg cggcggaact
     5161 gatccgggag aaaattctgc agcagaccta cgaagaggtg cctcactcgg tggcggtcga
     5221 gatcgaggag tttatcgaga ccaagaaatt gaccaagatc ggcgccgtgg tgctggtcga
     5281 gaaagagtcg cagaaaggca ttctgatcgg caaacacggc gaacgtctca agctggtggg
     5341 gacggcggcc cgggaagata tggaacgcct gttcgggatg aaggtttttt tgaaggtctg
     5401 ggtcaaagtg cgcgaatcct ggcgggaaga cgaacaaacg cttgcggagt taggttatta
     5461 acgacctcac catccaaccg cccgagcctg cgcgacttcc tgatcgggag ccgctcggga
     5521 aagatgcgtc tcgtgacgcc ggcggcggac aggctaagcc cgacgtgcgg ctggtctcca
     5581 ttcagcggct tctgcgccgt ggcgccatca ccaatctggc caagatgctg gcgcgcatgc
     5641 atccggcgga tatcgccaac gtcatcgacc atctttcctc actgaaagaa aaacgcgaaa
     5701 tcttcgaact ggtgcgcgga gacgtcaagc gtgggcaggt gctcagcgaa ttggacggcg
     5761 agagcatcac gctggtgttg tccgatctgc tgccttccga tgccgcctgg ttgttaaaag
     5821 acctcggttc cgacgatatc gcctacattt tgagtgtgat tccgaacgat cgcgccaagg
     5881 agatcctggc gctgatgcgg acggaggact ccactgaaat cgccgacctg ctgaagtatc
     5941 ccaaaggcac ggccggcggc atcatgacca ccgagttttt cgcgctcttc gaggatgcca
     6001 cggcgcagga agccattcgt cgcttgcagc aggccaccga cgcggaaatg gtgttctaca
     6061 tctatgtaac ggacaaggag gaccggcttg tcggtgtgtt atcgctccgc caattactga
     6121 ccgtccctcc ggcgactccg ttaaagaaca tcatgacccg ggatctgatc agcgtggccg
     6181 tggacatgga tcaggaggag gtctcgcgcc aggtcgcgag ctacaacctg ctggccattc
     6241 cggtggtgga gaaagacggc aaattggtcg gcatcatcac ggtagacgac gtcgtcgatg
     6301 tcatccgtga agaagcgacc gaagacatgt tgaaaatggc cggcgccatc gaagaggatg
     6361 cgatgttcaa gtcctccagc atggccgctg ctcgtgtgcg tctgccgtgg ctctttacca
     6421 atctcgtcgg gagcctcctg tccggcatgc tgttgtggat gttccggtat accattcagg
     6481 aagtggtggc catcgtgagt tttatccccg tcatcgcggc catggggggc aacgtcgggt
     6541 tgcagtcctc cacgctgatt attcgagggc ttgccaccgg cctggtggaa ctcaccgacg
     6601 tgtggaagat tttctttcgc gaagcgaaga ttgccttttt gatgggcatt gcctgtagtc
     6661 tgatgctgac ggtggtgggc tggttgtggc atcaggtctt tttgggcatg gtggtcggtg
     6721 tgtcgctcat tacggcgttc ctggtctcga cgagtatggc gacggtgatg ccgattgtct
     6781 tgaagcggat gggcgtggat cctgccgtcg ccgccggtcc gttcgtgacg acggccaatg
     6841 acatcaccgg cattgctatt tacctcacgc tggcgacgat cttcctcgag caaattcgat
     6901 aatgatgcgc ggtgggtctc ctcgtttccc tgtcatcctt cacgctgggt ggtgagatgc
     6961 cgttggtgaa gactgctgcc atcgtgctgc acagccgaaa gtggggtgac gccgaccgga
     7021 tcgtcacgtt ctatacgatg cgtataggca agttgcgcgg ggtcgcgcgc ggcgcgcgtc
     7081 ggttgaagag ccgtctcggc gggatgatcg agccctttac cctctgccag ctggatctgt
     7141 ttgagaagcc cggcgattca ctctaccgga tttcacaggt cgcgctggat gagccgttcg
     7201 ccaaatttcg agacgatctg accctcatga ccgccgcggg gcgcatggtc aaccttgtga
     7261 gtgccgtcat ggcggaaggc gatccggagc cgcgggtatt tgagatgttg gagtcgggat
     7321 tgcgcacgtt gttggagagt cgggacgcgg catggacgac cttattgttc cagattcgtc
     7381 tgttggggat gacggggttc aggccgcaaa ccgagcagtg cgcgacctgc ggccaagtgc
     7441 atcgaagttc gctcccgcaa ttttcacccg tggccggggg gatcgtgtgc gagcggtgcg
     7501 cgagccgtca gccgtttcgt tgtacggcgt tgtcccgcgg aagcgtcgca tttctccatc
     7561 aagcgttgca tatgcagccg gcgctcttga atcgattgca tgcggccggc caggtgcgga
     7621 ctgaagtcga gtcggccata gaaagttatg tcacggtggt ggcggggcga cgacttccgc
     7681 cggtcgattt tttgaccagt ggaagccctg cgtaaaaggg catccggttt gcgtggtgca
     7741 ggtgttttcg gaagccgagt aagggagcgt tgcctggtat gagtgagacg gtgcttgaac
     7801 cgaccgacat ggaatgggtg gcgaagggtg tcttgagcat cacctggagt gatgggcaca
     7861 aggcgttcta tcctgtccgt tatctccggc agcattgccc gtgcgccgcc tgtacggatg
     7921 agtggacggg agagcttcgg ctgaaaccgg atgacgtgcc gatggtgatt atgttgcagg
     7981 atgtgcagcc ggtcggacgg tatgcgttcc aatttacgtg gagtgacggg cacgatacgg
     8041 ggatctattc ctatacgttt cttcggcgga tgtgccagtg cgatgtgtgc cagccggtga
     8101 aacctgtgga gccgcgctcc agacggttgc tctgatcggt cggacgcagg tgtatgacgg
     8161 aacgggggag gtctactcag atgacacaag cgcgaatcat tgctgtggga tggtggtggc
     8221 ttgtcgctat cctctcggtg agctgttcaa gccttccgtc attggatcag caaaaacagc
     8281 tggttcagca gggggactat cgaatccatc agctgacccc gagagccttc gttgaaacct
     8341 ggggggaacc gacctatacc catcagcagt tcacgcattt cttcgggatg caggacggac
     8401 agctgattcc ccaatcgcgc atggcgctgg gagaatctcc gcagggatgg gagacggggc
     8461 ttgccgcggg cgatgcgtta ttcctggcct atgccgatcg tggccagtat ctggtgtttc
     8521 tcgatgaggc gctcgtgtac catgaggcga tgacgcccga gaaggttcat gctgtcggca
     8581 agacctggaa gtacgaatct cagttcaaga cgcgactcga attatccccg gccatgaagt
     8641 aagtggtggc ggctcctcct atgatggctc ctccttccaa tcatcgtccc ctgaagatct
     8701 gtatggtgtc ctccgaggtc gtaccctttg cgaagacggg cggactcgcc gatgtggtcg
     8761 gagccctcgc gactgagttt gccaggttgg ggcacgatgt ctgcgtgctg ctgccggcct
     8821 atcgacaggt cgatgcgctg ggctatcaga tggtcgacta tgcccggttg gcggtgccga
     8881 ccgcgcaggg gctggtagag gcgcgtatcc aagagctcac cggcccgcat tcgcatgtga
     8941 caggttctgg acgcctgcga gtgttgactg ttcgccacga tgcattcttt tcccgctcag
     9001 gtctctacca ggaggccggt cacgactatc ccgacaatct cgagcggttc gcattttttt
     9061 gtcgggccgt catggaattg ttggtgcact tcggtgaaaa ggacggctgg caggcggatc
     9121 tgctgcatgt gcatgattgg caagccgccc tgtgtgcagt ctacttgcgg acgctctatc
     9181 aagcgaagtc cgccctcagc aatatccgca gcgtgctgac catccataat ctgggatatc
     9241 agggattatt tccggctgaa catttttccc tcaccggcct gcccgcccat ctgtttaccc
     9301 cagctgcgct tgagttttac gggaaactga atctcttaaa gggagggctt gtcttcgccg
     9361 atctgctcac cacggtaagt cccacgtaca gtggagagat tcagacgcca gaatatggtt
     9421 gtgggctgga aggggtgatc agcgggcgga aggacgtgct gcacggaatc gtgaatggca
     9481 ttgatgctga gatctggaat cccgccaagg atccccacct gccgacccag tattctctgt
     9541 ccgatatgtc cggaaaggcg cgatccaaga aagggcttca gcgtgaactg aagcttcgta
     9601 ccgacaaggg gccgcttgtc ggggtgattg cgagactgac tggtcagaag ggtatcgatc
     9661 tcgtgatcga tattattccg gaactgatgg aactcgatgt gcaggtcgtg attctcggga
     9721 ccggagatgt gaagtatgag cagctggtgc gtgagttggc ggaacggtat cccgggcgac
     9781 tcgcggtgcg caacgtcttt gacgaagggc tggcccatcg gattgaagcc ggcgcggata
     9841 tgtttctcat gccgtcccgt tatgagcctt gcggcctcag tcagttatac agccttcgct
     9901 atggtactgt tcctatcgtg aggaagaccg gagggttggc cgacactgtg gtcaactata
     9961 cgccgagcgc tttcaaggga tcgcgtgtca ccggtttcag ctttacggat accagcgcgg
    10021 actcgctttt gacctgtctc ttgcttgcat tgtcgatcta ccggaaaaag gcggattggc
    10081 aacgcatcgt cagggcgggc atggagcaag atttgtcgtg ggctcgatca gccgagatct
    10141 atctgcggtt atttcaggag ctgcttgaag ggaaagagcc gcagcggaag tagggctcac
    10201 ctgcccgaag cgtagagact accggagagc ggcctggagt gtgaggtcga ggtacgacag
    10261 ccgttctcga gcttgcgagg cgtagtaaga ataatcggat tgtgtctggt tttctagcac
    10321 ctgcctgaac tcatttcttg ccagatcatg ttctccgact gacacggcca gattcccagc
    10381 atgtaggcta caggcagcgg ccatggcatg aacgtccacc gccagtctcg aggccggctg
    10441 ggtcaccatg ctcttcagct tggtcgggag gaagttttcg agtggtgagc ggagagcttg
    10501 ggcctgactg acctcgcgta atttgtgcga atcgaggagg gccgattggc tttggttcga
    10561 ggccaagcaa tgtgtgtagg tgctccacac atccataaat cggacattat cgtatcgaac
    10621 cgaggtgatc gcagggttgg attggcaacc aacgaccgac agggcagcga ccagaatggc
    10681 gaggatgccg actctgatgt ctcgtctatc gctcatgtgt cgcctcctcc aatccgtgtc
    10741 tctttgtcga gacagcaagg tattgagcaa gtgtcgggcc acggtttgca atcgtaacaa
    10801 attgaaataa ttgaccttaa attcgtattt tgaagtcgtt cctgtctcaa taatatgtca
    10861 ctgtgttgaa tagaacacgg aagatgagag ccggacaggg atcttgaatg accaaacggt
    10921 ggtcgcagcg tgcctttcct ggcggtcgac ccggccgtcg agattgcgcg gatcgctttg
    10981 tggcgtgatg ggtagtgtct ggtgtagctg agtattcagg gctggttggc gttgtcctgc
    11041 gatgttctcg gtggtacctc gcaaattcgg tgatagagag cttctccatg accgagtccg
    11101 tgctcggcca tcgctcgcag gacgacggat gcgagatggt attcctgtga cgatgcggtg
    11161 gcggcagtgc ccgtcactgg gctactattc tgcaactggg cggcggatgt agcgaagtgg
    11221 ggcatcagtg gtagttaaag atgaaggcgc ccattgttgg ctgaatgccc ctcacatggt
    11281 tcgggtagag tgctggcggt tgtggctcta gcgaattttg tgcgtggctg atattgtcgg
    11341 ggactcgtgg tgtgcatgtc ctctgcagtg tgtctggtca tgtaacccgg cggcacgcgt
    11401 tgcttcaagc agctggttcc gcagatgggg cggttgttgc catccgactg cctgaccgtt
    11461 ttgacatcta tcccagcagt caaaccgtat cgagtgccat ccttttggtg aaaggttcct
    11521 ccgctaatga atgcgagcca agcgttgacc aagacacgcc aattcagaaa cctgctcctc
    11581 tctgaacaac tgcagttcat ctgcgaagcc cacaacggac tcagcgcaaa aatcgtccaa
    11641 gaggccgggt tccccggtat ttgggccagc gggctctcca tttcggctca gttcggtgta
    11701 cgtgacaaca atgaggccag ctggacccag gtgcttgaag acttggaatt catgtccgat
    11761 gccacccgca ttccgatcct ccttgacggt gacacggggt acggcaattt caacaacatg
    11821 cagcggctca ttcgcaaact cgaacagcgc aacatcgccg cggtgtgcat cgaagataag
    11881 ctgttcccca agaccaacag ttttctcaag ggcgatgcgc agccgctcgc cgatatgcag
    11941 gaattttgcg gcaaaattaa agctgggaaa gatgcgcaga ccgaccctga tttttgcctg
    12001 attgccaggg ttgaggcctt tatctgcggg tgggggctgg cggaggcgct gcggcgtgcg
    12061 gaggcctatc atcaggcggg cgcggacggc attctcattc atagcgcgct ctcggtgccg
    12121 gatgagatcc tcgcgttcaa gcgggaatgg ggcaatcgtt gtccggtggt gattgtgccc
    12181 acgaaatact atgcgacgcc gacggatgtg tttcggcagc atggtttttc gatggtgatc
    12241 tgggcaaatc atatgctgcg tgcggccgtg gccagcatgc agaaaaccgc gcgcgcgttg
    12301 aaggagagtg agcatctcct ctccattgag gataaggtcg cgccggtctc cgaaatattt
    12361 cggctgcaga atgccgcgga gttgctggag gcggaagaac gttatctgcc ccgaggtgcc
    12421 gagggcacct cagcggtggt attggccgct tcccggggag aggaattggg ggagctgacc
    12481 gaggaccaac ccaagaccat ggtgaaagtg cagggcgttc ccatcctgag ccatatcgtc
    12541 gatgcgtata atgcggtcgg gattaaagat attttagtcg tccgtggcta taagaaggag
    12601 gcggtgaacc tccccaactt gacgtatatc gacaatctcg aatttgctga gaccggcgag
    12661 ttggcctcct tgtcccaggc attgcaatcc agaaggggtc gtttccagcc gacgatcgtt
    12721 tcctatggag atgtattgtt caataaatat attccgcagg cgttgtgcca ggagcaggat
    12781 gactgcgtga ttttcgtcga cagcaactgg agagatcaga gcagttatgc ccgcttgggc
    12841 ggttttgccg aatgctccat tccgaattcg cggaaagcct tcaatgccaa aatccatctc
    12901 aagcaattgg ggaagaccct tcccgaagag tcgatccatg gagtctggat ggggtttctt
    12961 aagctctctt ccagcgcggc cagccaggtc aatgatctcc tgttggacat gcttgccgat
    13021 ccggcgaatc gaagggccgg cattccgcaa ttgctgcagg cacttctgaa acaggacgtc
    13081 ccggttcgtg tgttgtatac ggtcggtcat tggttggatg tcaacagcct ggaggatgtt
    13141 gtccaggctg ggaatttctg agtttccacc caagtagatt cggttaggtg gttatggccg
    13201 gatgaaaaat cctgtctgca gtagaagcac aggtgaggtc cagctacacg aggcgtccgc
    13261 ctcaggagat acgacggcat gattgatccg caggatttta ttaagcacct tcaggccaat
    13321 ggggtggagt ttttcactgg ggtacctgat tctctgttga aggagttctg tgcctgcctt
    13381 gaacatctgc ctcgtgccgg tcatcacatc atcggtgcta acgaaggggg tgctgtggcg
    13441 ctggcactcg ggtaccatct cgcaacgcgg aaggttccgc tggtctatct gcagaattcc
    13501 gggttgggaa acatcatcaa tcctcttttg tcgttggccg accccgaagt atattcggtt
    13561 cctctcttgc ttgtgatcgg gtggcgagga gagccaggtg tccatgatga accgcaacac
    13621 aagaagcagg ggagggtgat gctctccatg ctcgaagcga tggaaattcc ccattccatt
    13681 ttggggcctg agttagttga tccgcaggca gcgctggcgg atgccctggc tcatgttcga
    13741 aagatgagcg caccgtatgc gctcgtcatc aagaaaggga cttttaaacc gtttgctgta
    13801 cccaaggttg agaaaacaga gtttccgctc tcccgggaag aggcgattca gcaggtgatc
    13861 gaatcgttgg gcgaacgcga tgtgctcatt tctacgaccg gcatgccatc gcgtgaagta
    13921 tatgagtata ggagcaagcg gggggaaggc catcagcggg atttcttaac cgtgggaggg
    13981 atggggcacg cttcccaaat tgccctggga attgcgctcc aaaaaccggg acgtcccgtc
    14041 tattgtcttg atggagatgg cgctctgctc atgcacatgg gggctcttgc tatcactggc
    14101 gctctcaaac cccgcaattt taagcatatc attgtgaaca atggggctca cgattcggta
    14161 ggcgggcagc cgactgtggc ctttgatgtc gatgttccgg gcattgccca tgctagcggc
    14221 tacgaatcgg tattttgtgc tcaaactaag caggaacttc agtcccgtct ggcagagtta
    14281 cagcgttcaa gtggtccgag cttattggag gtccgagtac gctgtggtgc caggaaggat
    14341 atcgggcgcc cggtaacaac tccaagtcag aacaagaatg cgttcatgga ttttgtcgag
    14401 aattgaggcg tgaggtggtt cgctcgagcg ttttgtgcag gatggtagtt tgggcgaagc
    14461 tcagtctagt aagtgagcgt caggtctgtg gctggctccg attgaaatcc ataatcttat
    14521 tctcgcagga tgacaactag aatgctaaga tcggaacact tggactagaa ctcctgtccg
    14581 agtcgttgaa tttgtctttc gcataatgtg tttgatgtgt tccttcactg tctgctcggt
    14641 gatcttcagc gcgttggcga tttccttatt ggtccatccc tttgccagat tttcgaccac
    14701 tgattgttct ctgtttgtga gctgaaaccg ttccttggct tgatcggtat taagttgctg
    14761 ccggcggcca agttcttcta gtgttacgac aaggcgggca tactggatgc cacctcgatc
    14821 gggtaagcca aatcctcgaa ggaggacggg ctgatttggg tttcctgcga ttcgtttgac
    14881 ctcaaattgt tcccagtcct ttgcctcgga gcgaacttgg agtgctttca gaacttcggt
    14941 gcagagttcg gtcaaggcag cgggaagcac cccctgggcg gactttgcag gtgcgccacc
    15001 gttttcagcc atattgatga gcttggacag ctcagcggct tgtcgattca tatgcagcag
    15061 ttgcatggag gaggataaaa cgacgattcc ggcacctgca cgttggtcag ccaggttatc
    15121 tgcaggttcc tgagagtgaa cagcgttttg agccacggtt atactccgta tggtccttaa
    15181 tctctctaga caatcaggct atttctgata acctaaagca tgtgtctgtt actagtaatg
    15241 ctacataata ctttcgaggt agtcaaatta tacttgtggg ggagttatcc gtacgtcatt
    15301 ggtattgttc gttggcgctc gtacttctat actgcaaaag cagaggcaat tatcgttggt
    15361 tagggcagct agcatgagat gaattggtaa atgacttcgg tagccggtgt tcaatgggcg
    15421 taccggatct gcccatcaac cgtaaattgc ttagttatta atggtatagg ccatggtggg
    15481 ttccagccag gcgctttgta aagttttgtg cggtaatgag gtccctagag atgcgtgatc
    15541 taagcagtca aaatgggcct gtagcccagg acggatggga ctggcccaca attgcggcca
    15601 gcgagaagca cgccgagcgg ctagcgctcc ttcgtccaat tccttatgaa atgtccctac
    15661 cgaatcatga aagtggtcaa aacggaccca gcgtgttcca cggacgggcg ctctcattga
    15721 acattagcag tggtgggatg ttgatcctca tggctcaggc gccactgctt gatcaggtca
    15781 tcaaggtaca tgttccgacc cctgttgcgc tggccgagac cccaacactt gcggaagtgc
    15841 gctggattag aaaagtcccc ttttcagcgt cgggaagtga ggcatccttt ttcgtgggtt
    15901 tgaagttttt gttctcggcg ggaaggtaga tgctaggcag ttgttgcaag tggccagctg
    15961 gaatttcttc aggagaataa gtcatgaatg gtaaggcctt attgagcggg attatccttg
    16021 tgggaaatgt gatcggtgtg gcgcatgccg gacaatttga ccaggtgccc aagccgacgt
    16081 tagtagcggg atcgtctcca ggggcaagtt cgtctggggt ccgccacgga ggcgaagggg
    16141 cagaaaaatc atcccttacg gtgaccccgg attatattat gggcccagaa gatgtgttag
    16201 agattacagt ctggaaaaat gctgatcttt ccaagcaagt tcaagttcga cctgatggaa
    16261 ggatttcact tccgctcatt ggcgatgtct cagcagtggg acgaactgcg gctcagctga
    16321 ccgaagagat ttctgctcga ctgaagtctt acatggaaaa cccgacagtt tcgatcttgg
    16381 ttcgagaggt aaatagttat caaatttatg ttcttggtga ggtgaatgca cctggtaagt
    16441 accctttaaa aagcaaaaca accttgctgc aagcgattac gatcgcgcat ggttttacgc
    16501 aagtggctgc gcgcaacaag attgtggtat ttcgattcgg gaaagacggc gaggggctga
    16561 acaagattaa ggcgagttac gacgatattg ttctgagaga tggatccgat caaaacatag
    16621 agctaaagcc cggagatcag attgtcgtgc cttctgaaac gatggtggtg ttgccgtcaa
    16681 ggtagagcct cgtcggtcga atttacgggt gacatttcgc tgaagcgagc caaaggagtg
    16741 gggaaattga aaactttcgg ttgcagatgg ttgcgagggg gggtatgtat tgttttcagc
    16801 ctgcttctac ctctcccagg gtgctggatc gggaatgaac aaatcgactt taatgtcacc
    16861 tatatcccgc cgaatgagtt cttgttggga ccggaggacg ttcttgttgt aaatatttgg
    16921 cgaaatcaag agctgtcccg agaagtcatt attcgtcctg atgggaaaat ctccatgcct
    16981 ttaattggcg atgttcaggc cgcaggaatg acatccaatg tcttggcgaa gagaatcgca
    17041 gatgggttgg ctgagtttat gtccaatccg acagtgtcgg tgcaagtcaa agaggtcaat
    17101 agttattatg tgtacgttct cggcgaagta tcaaagccgg gtaaggtgcc gctaaagtcg
    17161 tatgcgacgg ttttacaagg catctcgttg gctggaggtt tcacaacgtt tgcttcgaga
    17221 aataaaatcc atgtgcttcg ggttgttccc aatggtcaag ggcagccgaa acaggttcaa
    17281 attcctgttc cctatgaaga cattattcaa ggtaaaaact cggagggaaa tttcttcctc
    17341 aaggcgggtg atgtcattgt tgtgccgtga ccagtgcttg aagataggtt ggttgtgtta
    17401 cggcgttttt gccctggtgt tgtgttggac tatatccgac tctgccgcgc aacagatacg
    17461 cgtggtgccg tcaatctcgg ttatcgagca gtacgacagt aacgtatttt tcacaccgaa
    17521 gtcgcaacta ccggcaggaa cgaaagttga tgatctcatc acgatagtca ctcctcagct
    17581 aaacttcatt caaggcaata cgcttgtgaa gtcaaatctg tcagtcgggg cagtcgttca
    17641 aaagtttatt aacaactcag agcttgataa cgtgggattt aatgcgagcg ctgggattga
    17701 tctctcgcaa gctgtgaaca gggttttgcc acgaatgcgg ggcttccgag tgtttgggac
    17761 ctatcagtac atgccttcga ctccagcttt tggcgctgga ggtctcggcg gagggttcgg
    17821 tgggggagga ggatttggcg gagggttcgg gaccggaggg atcgggatcg ggggacctat
    17881 tgattctgga cttctcacgc aacgaatcag aaccaccatg tacaatgctg gattttcgga
    17941 ttcctattcg ctctcaccga ccactgattt tcaaactagc tacacctatt cccagcttag
    18001 tttcggcggt gcactcacgc cgactacgac aacttcggga caaccattaa atacggtgtt
    18061 tgatactacg actcattcca ttaccgcagg accgacatct cgaatttctg ccgtcgatac
    18121 cttgaccgtg agatatacgt tcacgcaaat gtctcaagca caattcggcg actacgcgac
    18181 gcatggcgga acacttggtt ggggaagaag ttggaccaag gagtggagtt cctcagtcaa
    18241 tggcggccta acacttattg agccaattcc ggatacctct gttgctggag ggcaacgaag
    18301 aattccagca acaatgtttc cgacgggagg ttttagcgta agttatgctt cgggttcatc
    18361 ttttcttagg aaactaggca gtgaaattca agaggcaacg ggaaccagtg gagccggtgc
    18421 aacaggtggt gcaggcttcc tcccacttct tgctgggatg aatatgccag gggggatcgc
    18481 agctcccgga tcttatcgag tcaccctcat gtataatctc ggggtgtttc caagcttcgt
    18541 tcaatctgcc ggaccgatct atacacatat ggtttcactg ggaagcatgg cgggaatcac
    18601 taacaagcta agtgctcagg caatgtttaa ttatgctagg agtacttata cctcagatgc
    18661 tattggtacg actttcagca gctatggtac gacagtaact ttgaattatc tgatcacgcc
    18721 catgtttacc gctaggctaa gtcaccagtg gcttatgttt gacgctgaga cgagtggttt
    18781 caatgctgcg gatttcggat ttgcgaaaca aatgattctg ttaggtttca catacgcgta
    18841 ttcaccacgt ggagattttt tccggtctgg tgccttctgg gaaggttcct ccggtgcctc
    18901 ctcgtctgct gagtcaggag tgagtaaatc tggatcagga ggagtggata caaagaaatg
    18961 aacacgcgca cattgtctcc agaggattat tggcgagcgg ttgtcagcag aaagtggttt
    19021 gttatttctg caatcttggt ctcgctgagt attgctggtg tggtatgtgc gctcacgccg
    19081 aagatttatc agtcggcgac caaaatgtgg tttgagggtg cgaagatcga agagtccatt
    19141 gtgagcgggc ccactccagc tggaagcggc tattccccta cactggatga tcgcgtcatg
    19201 gaagtgcgac agtttgtgat ggggagaaaa actcttgggc aaattgcggg agagtttggg
    19261 ctgtttggat acgaaaaaga tcatccggat gctccagaat cagagaatgc catcagagcc
    19321 atgcggggat cgattaaggt tgagcctacc aaggacaagt tatttatcac tctttcattc
    19381 tctaatgaag atccgatcat cgctcgagat gtgacatcaa gacttagtga tctgtttatt
    19441 gaggagacat tgaaggatcg ggagcgaggc gttgaggcgg cggaggattt tcttggactt
    19501 gagttaaaac atgcaaaagc tgagctcgag gtaaaggaaa aaattatttc agagttcaaa
    19561 caacaacatc tcggggaact tccgcagcag atagatgcca atcttcgcaa gctagatcga
    19621 cttcaggatg acatgcggtc gcaaagtgaa caggcccaga atctggccaa tcgattggcc
    19681 caaatcgaca agtcaattaa agattacgag gagactggag aaatcagtag tgattcgccg
    19741 gtcggcggtt ccagcaagcg caataaggat ccacgccttg ggagaattaa ggaattagag
    19801 cggcgcttgg tggagctgtc ctcgatatat aaggagacct atcccgatct ggtacaagtt
    19861 aaggaggaga ttaagaaact ccgagagatg acttcagctc aatatcgcga tttgttgcct
    19921 gatagcgagg gcgttgaaga accggctgtt gggaaaaagt ccaagcgcaa ggcaattgat
    19981 ccataccatg ctgaacttct caagcagcgt gaggagattg ttcttgagtt ggatgcggta
    20041 aaacgtcacc aggcgcatat ttctagtgaa atttctcagt atgaacggag ggtggaacat
    20101 accccagagc gggaacagaa actaaagaca cttgaacggg actatgaaaa ccttcaaaag
    20161 aattatcaat cgcttctgga caagaaactc agtgccggta tgcagaagag tttggcccaa
    20221 ggacgcaagg gagccaagtt tagcatcgta gatccggctt ataccccggt cgttcctgtt
    20281 gtgcctaaca ttccgttgat tatggcggcg ggacttgtcc tgggctgcgc gttaggtttt
    20341 ggtggtgccg tcggtcttga gcttatggga cgaggattcc ggtctgcgga agaggtcgag
    20401 ctgactttgg gccttcctgt gattgcatca attccactgt acgaaagtgc gttcggcgga
    20461 tcaatgcaaa ccgtgcgggc actttctggc aaaagccgaa atgctgcggt gttgttaccg
    20521 cgttatggaa aacagggtga aagtctggag atttctggag gccttcctgt tgcatctgtt
    20581 agcaagtcca gaggtgccaa tgggaaacta aatgacccaa ctcctggtct ggagttggtc
    20641 tcaatgtggc gaccgctttc atttgtggcg gagcaatatc gtgttgcggc cacccggctt
    20701 gagttgatga caggggatcg aaagagtacg gcgatagttg tgaccagtgc tgtcatggga
    20761 gagggaaagt cctctacggc ccttaatttg ggctacgtgc tggcgaaaga ccttgatcgt
    20821 agaaccatag tgatcgattg cgatctcaag cggcctatgc aacatatcta tgcaggagtc
    20881 tggcagcaac cgggcctggc tgaagttctg cgtggcacca aggtggtcga agattgcttg
    20941 cagagacttg gggaggcggg cccatggatt cttaccgcag gggcggtcgg ggataacccg
    21001 ttggccttat cgaaaatgca tcagttggct gatctgattg ctgaactgaa ggaaaaattt
    21061 gattacgtga tcattgatgc tccgccggtg ttgccacttg ctgacatgca ggtgttggca
    21121 agcatgggag atcttctcgc ctatgtggtg aaggcaagca tgactggtcg cgatgttgta
    21181 caaaaggcat tgagggtcat cggtgatacc gccaatgtgg ggattatctt gaatggctta
    21241 gatgcacata ccactcccta ctatatgcag caggagtatt accgcgaagc tcaccatgaa
    21301 cagctcaagt aagtccgaac gggagatagg cctagagcct gtggtcactc aaccaccact
    21361 ggtttcagtg tcggttagct ttcctaaatt cacgcggcga gtgttaattc taggcgttgg
    21421 gcctcttgct cgagatctct gtcagactct tctgtctaaa cgaaccggtt ttaccgaagt
    21481 agtggggttt cttgataagg atgcgagtag ggttggtgag cggttggtaa atccgagtat
    21541 catcggtacc tacgatcaac tatttgagat tgcggagcga tatcaagttc ataccgtggc
    21601 cgtgtgtttg gaagatcgtc gcgcggttct gcccgttcaa acgctattgg acatgaaggc
    21661 catggggcgt gatgttgttg atggccacta tctgtatgag gaggagtcgg gccgcctttc
    21721 gattgaccat ctcaagccga gtgcactcat tttttcgacg gggttccgtc gtcgattagt
    21781 cacgatgctc ttgaagcggt gcctggatgt tttggtggca atcgtaggaa tagtcgcact
    21841 ggtgccgctg gtattgcttc tctctatcct tatcaagtta gactcatgcg gtcctgtttt
    21901 ctaccggcaa atgcgagttg ggcttcgcgg ccgcccttac atgatctgga agttcaggtc
    21961 catgcgacag gatgctgaac aaagcggggc gagatgggcc acaagtgaag atcctagggt
    22021 atccagagtt ggtcgatgga ttcggaagtg gaggttggat gaactgcctc aactgatcaa
    22081 tgtgatgaga ggcgagatga gtttggttgg cccaagacca gagcggccgg tgtttgtgca
    22141 ggaactcaga aacaccatac cttattacga tctgaggcat accgttcgtc caggaatcac
    22201 agggtgggca caaacccgat ttcggtacgg tgcatcgaag gaagactcgc acgtcaagct
    22261 tcaatatgac ctcttctatg tgaagaattt gtcactggcg ttggatttgc gaattgttat
    22321 tcatacagtg aaagtaatgt tgatgggtga gggggcacgg tagggggcgc aaatgccgga
    22381 tgcaattcct agacattgtt tgtcatttga cgtcgaggag cattttcagg tttcggcatt
    22441 tgaaacccca atgcgaagaa ggcactgggg gcaattcgag agtcgcgtcg aagccaatac
    22501 tgagagactg cttggattgc tggaaaatag aggcgttcgc gcaacctttt ttattctagg
    22561 gtgggtagcc gagcggtatc cttcgctagt tcgccgtatt gcatccggcg gacacgaagt
    22621 ggcttcacat gggtatgccc atgaactaat aacaagccaa actcctagta cttttcgcga
    22681 ggatattcgt aaggccaagg gtatcttaga gaacattctt tctcagccta tgcttggcta
    22741 tcgcgcaccg agcttttcca tcaccaaaga tacgatgtgg gcgactcaga ttctggttga
    22801 agagggctat gtctacgatt ccagtatttt cccggtactc catgatcgct acggtgtgcc
    22861 ttctgcaaac ccggaggcgc atcaactgtt gaccgcatca ggtctcctgt gggaggtgcc
    22921 cccctcaacg gttaagtgct tgggggtgcg gctacccgtt gctgggggtg ggtattttcg
    22981 gttgtatccg tatgcactgt tgcgtgcgct ccttcgcaag ttggaagccg agggggcttc
    23041 actagtgatg tatatgcatc cgtgggaatt cgacccggat cagccaagaa tggagggttc
    23101 attggtgtcg cgaatgcgac actacttgaa tcttgataag accgaaatcc gaatgcggga
    23161 actcttgcaa gatttctctt ttgctccaat tcgagaagtc tttccccaaa ttgaacaaat
    23221 gcacagtgcg cggcttgttt ctcaggtttc gggagtgtag gtgcattctc ggaagagtcc
    23281 gtcagcggca ttacccagct agtatccttc tcggggcaat ttttgaaact gacaataccc
    23341 agacttcact gacgctttgt tgctcgcgac tagatagttc ctacttatat cgacaaaact
    23401 caaactgtgc taacgttcag caatcgcatc gtgggtcgac ttcttgccta ttggggcgca
    23461 ttgggagagt ggtatcagca gctcccgtcc caagtcggtt ctctcacatg cataagcgcg
    23521 ggcgctggta gttcaatagg atggttttcc gcctctcgag aagcgtattt gcttgcctcc
    23581 attgagtgtg cactattctt cgatcataag cagtgcattt gattaggata gaaatcattt
    23641 cctgccggct gtgctactct tgtggtgtgg acccgtcagc attcgttgac acattccgct
    23701 tgggcagacc cacgaaaaga agtggattcc ggtcatttgc tcgagaatga gcagagaggt
    23761 agttgcgccg gaattttcgt cttgggtggc agaagccaat agctaggtat cacgatggaa
    23821 tctacagtag agcaagaaag catggccgtg ctggtaatag aaccccgcac aggttatgtc
    23881 caggtgggat ggggtgaatt gtgggctgca cgggaattgt tgtactttct cgcatggcga
    23941 gatctcaaaa cccgctatgc tcagaccgct attggggctg gctgggccct catgcagcct
    24001 ttgctcagca cgctgatctt cacccttgta ttcagctact tggcaaaagt gccttcggat
    24061 ggactgcctt atccactctt cgcattcgct gcccttctgc catggtccct gtttgcgagg
    24121 agcctggaac gcagcacatt gagcgtggtg accgaaggcg gcctgattaa gaaagtctac
    24181 tttccacggc tgatcattcc aatcgccgcc accttcatta atctcgttga ttttactgtc
    24241 gggttgctga ttctcatagg aatgatgacg tggtatcagg tgttgcccca gtggacggtt
    24301 ctgtttctac ctctattcgt tattgtcgcg ctgcttactg cattgtcggt aagtctgtgg
    24361 ctgtcggctc tcaatgtgaa atatcgcgat gtggcttcgg tcgtcccatt gatgacgcag
    24421 ttgtggatgt tcgcctctcc ggtactctac ccggcatctc tggtacccga gtcgctccgg
    24481 tggtactacg gtttaaatcc tatggcaggt gtgattgagg gatttcggtg ggcgctactc
    24541 ggcaagtcgg cccccgactg gactatggtg gcggtgagtt tgggtgttgt gagcttcttg
    24601 ctgattgggg gtgtcatgtt ttttcggagg gtggagcgaa ccttcgccga tctgatttag
    24661 atgagcaaca ccgctgtcag cgtcaccggt ctctccaagc gctatcgcct ggggacgacg
    24721 catgctcaag acggctctct ggccggagcc ttgacgcgtg ggctccgcca cctcatcggt
    24781 gggcagccgg catcccccca gacccatgac accctgtggg cgctgcgcga cgtgtcgttt
    24841 gaaatcaaaa aaggcgaagt cttcggagtc atcggcacca acggctcggg caagagcaca
    24901 ctccttaaga tcctctcgcg ggtgacggaa cccaccaacg gccgtgcgct catccacggc
    24961 cgattttgtg gcctcttgga agtgggcacg ggctttcatc cggaactgac cggtcgcgat
    25021 aacgtcttca tgagcggcgc gattctcggc atgaagcggc aggagatcgc ccgcaagttc
    25081 gacgagatcg tggcgtttgc cgaagtggag cagttcatcg acacgccggt gaaacattat
    25141 tccagcggca tgtatgttcg gttagggttc tccgtgctgg cccatatgga tccggacatt
    25201 ctgatcgtcg atgaggtgtt ggcggtcggg gacgtccggt ttcaaaagaa gtgcatgggg
    25261 aagatggaag atgtggggca gcatggccgg acggtgatcc tcgtctccca cgacatgccg
    25321 gccatcacgc gaatgtgttc ccgtgcgatt ctgctgaaca agggggagat cgtgcaggca
    25381 gggccggcgc atgaagtggt gaatcactac ctccatgccg ggcacattca acctgcggtc
    25441 gaatggctgg atccaatcca ggctccgggc aatgacgtcg ctcgccttcg tgccttgcgg
    25501 gtcagatcgg aagcgggaca agttgcggat gtcatcgata ttcgaaagcc tatgcgagta
    25561 gaaatcgagt atgacatcct caagccaggg cagatggttg ttgcgcaatt tggtttcaat
    25621 accgaagaag acgtgatcgc gttcatcgtc aacgaccggg acccagtatg gtacaggcgt
    25681 cctcgtccag tgggtcgata tgtcagtacc gcctggattc ctgggaactt tctgtcagag
    25741 ggaacaatgg tagtaggggg agggattttt tcggaagatc cttggcatga acattgtgat
    25801 gtgccgcgag ctgtgggttt tcgcgttgtg gatcccggga ccggggatac ggcacggggt
    25861 gacgtgacag gacggtggga gggggttgta aggcccttgc ttcgatggac aacagagcat
    25921 agctcgctat aacgagggaa ccagtagtca gtactggtat gctggctcct cgctgggttg
    25981 agagaatggc atcatgacaa tcgatagggt tgtgctgttg gtttttctgt gactggcaaa
    26041 taagctagca aatatgttcg cgggagtaac cctgttggca cacgaacagg atttcggcgc
    26101 gaataggggg tagtcgcctg tgtaaagccg gagatcgaga taaggctcac gtttcacaat
    26161 tcgttgggct gtggggatga tccccgtatg agtgaatgat gcagcgacga tgggaggact
    26221 taacgggaaa gctcagctgc accttcttgg cgagcagaat gctatttgag gaggtctttc
    26281 agcttaaaga tgttatggtg aagagtgatg gcaatgccct acctgccatg gtgagtttgt
    26341 cgtgcttgtg ggtacagaat tgggtcgagg cggcaaactc tgtgggtacg gctatgagtt
    26401 tggtgattga tgagctttcg atgatgttct gcgaatttaa gccttgtcct cgctgcagat
    26461 aggcatatgc tgggaaagcg aaggaagtgt actcgatgct ctgacgtagg tatccatgtc
    26521 agtacggatg gcgacaatga ttgattgctg gaggtaggga catgaaagcg gtgattttag
    26581 ctgggggatt gggaactcga ttgtccgagg aaaccgtgct tcgtccaaag ccgatggttg
    26641 aaatcggcgg caagccaatc ctctggcata tcatgcaaat tcatgcggtg tatggggtga
    26701 ccgagtttat catcgccctt ggctacaaag gggaaatgat caaagagtac ttcctgaatt
    26761 tctttgccat caacaatg
//
LOCUS       DAORQN010000011        24512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_140_length_24512_cov_21.729321, whole genome shotgun sequence.
ACCESSION   DAORQN010000011 DAORQN010000000
VERSION     DAORQN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_24512_cov_21.729321"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="thrC"
                     /locus_tag="PK614_01745"
     CDS             <1..693
                     /gene="thrC"
                     /locus_tag="PK614_01745"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRB80968.1"
                     /translation="MMHQATVIQVEGNFDQALTIVKELSATYPVELVNSLNPFRIEGQ
                     KTAAMEICDDLGDAPAMHVLPVGNAGNITAYWNGYREYRAANQTTRLPRMMGFQAAGA
                     APIVLGHIVEEPQTVATAIRIGNPASWQSALTAVKESSGAIDMVTDEEILHAYALVAS
                     EEGVFCEPASATSVAGVIKLSQAGQLKEGATVVCTLTGHGLKDADTAIGISRQPQTVK
                     ATRDDVARLLHV"
     gene            712..1962
                     /gene="apgM"
                     /locus_tag="PK614_01750"
     CDS             712..1962
                     /gene="apgM"
                     /locus_tag="PK614_01750"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRB80969.1"
                     /translation="MKYLILHADGMADLACPELGGKTPLQAAATPNLDQIAQRGEVGL
                     LSLPSEAGAAGSDVTSVAVLGYDPKKYYPGPGPLEAAGLGVTVGEQDVVFRCTMVTVR
                     GESASGSKDRATDIKKLGPHVLMEDATAGLIDTEQARELLEAVNEQLGSESIQFYPGS
                     GHRHLMVWVGGKSRATCLDPQPLVGRSIADALPSGDGADVLRKIMDAAFFILRDHPVN
                     EEREQEGLKPANCLWLWGQGRAAVWPPLPERYQIAGVVVSSSDVHRGVGVCAGLDAVE
                     LPLADGSDANEFTGCVQAAVQELAKKDFVYLHAGLSDDVLHATDVQDKVRAIERFDAQ
                     VVGPILAALATHPAYRLLVVCDPGLAKSHGAEVPPILYALCDSAAKPSAGVTKRFHEQ
                     DANIAGTAPRDATKLMLRLFPRGV"
     gene            1967..3202
                     /locus_tag="PK614_01755"
     CDS             1967..3202
                     /locus_tag="PK614_01755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRB80970.1"
                     /translation="MALIVQKYGGTSVGTVERIHRVAERVERAQKDGHQVVVVLSAMS
                     GETDRLLKLAHEVTGAPDERELDMLLSTGERVTIALLAMELRGRGVNARSFTGRQVGI
                     HTDSAHTKARISRVKADRIKEALSAGVIPVVAGFQGINASSDVTTLGRGGSDLTAVAL
                     AAALKADRCIIYTDVDGVYTADPNIVPAARRLDKISYEEMLEMASLGAKVLQSRSVEF
                     AAKYSVPVEVNSSFKEGKGTLVTREDADMEGVMVSGVTGDRNQAKITIVGVPDRPGIA
                     ARVFGAVANANIVVDMIIQNVSQASTTDISFTVPKADLRNAVDLVQRLSEEIGARSVA
                     VTESIAKVSLIGVGMRSHSGVAAKMFEVLSREGVNIMMISTSEIKISCVIEEKYLELA
                     MRTLHTAFGLDRVSAPALG"
     gene            3332..4921
                     /gene="cimA"
                     /locus_tag="PK614_01760"
     CDS             3332..4921
                     /gene="cimA"
                     /locus_tag="PK614_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRB80971.1"
                     /translation="MPPLTAAQASALEIYDTTLRDGAQAEDVSFSVEDKLRVAQRLDE
                     LGVHFIEGGWPGANPKDIEFFRTVKTIPFKNATIVAFGSTRKASNSVRKDKNLQELLA
                     SGTPTITLFGKSWSFQVTDALGIALAKNLELIEDSIHYLRSKDRRVFYDAEHFFDGYK
                     ANPDYALQTIRRAIAGGAERVILCDTNGGTMPWEIREICRIVKQECPVPLGIHAHNDS
                     EMAVANSLVAVESGILQVQGTINGIGERCGNANLCSIIPNLQLKMQRPALGNKLTHLK
                     DVSGFVTEIANLMPNKRQPYVGDAAFAHKGGVHIHAVLKNAATYEHIDPAAVGNRRRI
                     LVSDYAGRSGLLEKVEAYGISLNKNHAKLQELVETLKERESQGYQFEGAEGSFELLMR
                     KAMGSHRPSFQLLGFRVIVEKKQEHSLLLSEATVMVKVGEVVEHTAAVGAGPVNALDH
                     ALRKALEKFYPQLREVKLLDYKVRVLSADKGTESKVRVLIESGDNKDKWGTVGVSENI
                     MEASWQALADSIEYKLLLADR"
     gene            5067..5342
                     /locus_tag="PK614_01765"
     CDS             5067..5342
                     /locus_tag="PK614_01765"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRB80972.1"
                     /translation="MRKADIANEIFKQVGVSKNDAADIVELVLNLLKGVLQKGDAVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            5384..5737
                     /locus_tag="PK614_01770"
     CDS             5384..5737
                     /locus_tag="PK614_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRB80973.1"
                     /translation="MGNEPRLGSKVFYKIGEVSKISKLPAYVLRFWESEFSFLRPKKS
                     RGNQRLYVQKDVDTVLEIKRMLYDEGHTLAGVKRYWARRGRVTVKKLSSRKELAQRVR
                     GDLQAIIRMIDSHSS"
     gene            5797..5873
                     /locus_tag="PK614_01775"
     tRNA            5797..5873
                     /locus_tag="PK614_01775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5831..5833,aa:Pro,seq:ggg)
     gene            6040..6855
                     /gene="surE"
                     /locus_tag="PK614_01780"
     CDS             6040..6855
                     /gene="surE"
                     /locus_tag="PK614_01780"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRB80974.1"
                     /translation="MARVRILVTNDDGITSPGIHAVAAALGALGEVWIVAPDRERTAV
                     GHAVTLHKPLRITKMAPRVFMVNGTPVDCVNLALVKVLPARPSLIVSGINRGVNLGDD
                     VMYSGTVSGALEGTILGIPSIAVSQEGNETFRFDVGAQYAARVAAEVLRHGLPPETIL
                     NVNIPNVPVRSIKGVKVTCLSRRRFNNPIVEKVDPRGRKYYWIAGTRQSWSRENDADH
                     EALEQRMVSVTPIHLDTTHHEVLEQFKAWEQPLSRPSARPKTVKPHPRRRAQA"
     gene            6852..7487
                     /locus_tag="PK614_01785"
     CDS             6852..7487
                     /locus_tag="PK614_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRB80975.1"
                     /translation="MTGLIEWLIEVLGRFVIATISTFGYTGIVITMAIESACIPLPSE
                     IIMPFSGYLVSTGQFSMMGVTLAGAIGNVLGSLVAYYVGVWGGRPFVERYGRYVLVSQ
                     HDMELADRWFAKHGEAAVLIGRLLPVVRTFISLPAGIARMDVKKFVLYSFVGAVPFCY
                     ALAYVGLKMGEHWNQLRQYFHHADLVIGLCLAVGLGYFLWSHWPKRRPSME"
     gene            7497..8957
                     /gene="cysS"
                     /locus_tag="PK614_01790"
     CDS             7497..8957
                     /gene="cysS"
                     /locus_tag="PK614_01790"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRB80976.1"
                     /translation="MLRVHNTLTGSKEPFEPLVPGKVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGFVVEFAKNFTDVDDKIIKRANEQGVSCEHVTTTYINAYYEDMEK
                     LGVRRATLEPRATEHIADIVRLVDRLLAKGMAYRVDGDVYFQVDRYPVYGRLSKRNVD
                     DLQAGARVDVDERKRHPMDFALWKGSKPGEPSWDSPWGPGRPGWHIECSAMAMRHLGE
                     TFDIHGGGMDLIFPHHENEIAQSCGATGKEFARYWVHNGFVQINQEKMSKSLGNFFTI
                     REIFQKSEWSDTVTGEMLRYFLLSTHYRSPLDFSDQSLNEAKNALNGFYDLFERLNES
                     APAHGAADQQMQESTMRARAAFVAAMDDDLNTPNAVAALQTLRGEANKALEVGLSGEM
                     RRVVRQEFRVLGIVLGLLQTDTWRFKSQRQQPSSGGAEASTDTLSDEDIADKLAARLA
                     AKRSKNYQLADQLRAELASHGITIEDRPDGTSRWKR"
     gene            8932..9708
                     /gene="rlmB"
                     /locus_tag="PK614_01795"
     CDS             8932..9708
                     /gene="rlmB"
                     /locus_tag="PK614_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRB80977.1"
                     /translation="MAPVDGNDNSLEVIYGLHAVREALRAGVRPIQRLLVLGTDRQFG
                     EIARLARDQRVPVRVEPRAALDRLVPSGHHQGVVGVVAAKAYAEPDDILASAQAEGQT
                     PLLVLLDGVEDPHNLGAILRTAEASGVQGVFIPERRAVGLTSVVAKASAGAVDYMPVG
                     RVPNLSRLIERLQGAGIWVYALDAEAPKPYTALDFSGPVALVFGGEGTGVRPGVRDAC
                     DEAAHIPMMGNVSSLNVSASAAIVLYEALRQRREGAGKKP"
     gene            complement(9726..10016)
                     /locus_tag="PK614_01800"
     CDS             complement(9726..10016)
                     /locus_tag="PK614_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB80978.1"
                     /translation="MYMSPVAKTTKSRAKVSAPDPVLPPRKRRPEALTTREQEILELI
                     WTGFKNKEIAQRLKISVKTVEAHRANMMKKIRVSNTAQLLKAAIQGKMLKLR"
     gene            10232..11005
                     /locus_tag="PK614_01805"
     CDS             10232..11005
                     /locus_tag="PK614_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRB80979.1"
                     /translation="MISYVTVLLFGAVIGSFLNVCIYRLPREESVAWPASHCPSCRQA
                     IPFYDNIPIVSYLLLRGRCRACHAPIAIQYPVVEAANAIGYVLVFWAFGFTPAAWAYA
                     ALVSALIVVTGTDVSHTMIPDAVTLPGIVIGLLCAILILPIGLVDSLLGVLLGGGILW
                     FLAWISPYVFGKEGMGGGDIKLMAMVGAFLGWQPVLLAIMIGSFLGSIVGVGLIAIGV
                     MRREQYIPFGPFLAVGSILALLFHQPLLEWYWALIDIPQ"
     gene            11297..11731
                     /locus_tag="PK614_01810"
     CDS             11297..11731
                     /locus_tag="PK614_01810"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HRB80980.1"
                     /translation="MNERGGSLVELCTVVAIIGIVVGVSVPGWAALVAKHQQRAVMVE
                     IASELRMARQLAMARHERIRVVVDAEQSELRTECMDCDTSLLRRYEFAHKGTAVESMT
                     TKPEIMFQPSGRSATATTMVLVDGRRATHLVTVSITGRVTLS"
     gene            11752..12255
                     /locus_tag="PK614_01815"
     CDS             11752..12255
                     /locus_tag="PK614_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB80981.1"
                     /translation="MTRRSALMSAKGFTLVESMVALVVLSIGVIGTIGMCEWAERGLQ
                     RGALTTTALALAESRLEAKRSVAWERLLMDDVDQDGIVESVMRDDGLQDDQANGDGIF
                     TASATQSNIRLVWTVELNRGHEPARASLATIEARAIFRTMGGQEKEVRVRTIRANPRY
                     VGLSVLS"
     gene            12264..13028
                     /locus_tag="PK614_01820"
     CDS             12264..13028
                     /locus_tag="PK614_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80982.1"
                     /translation="MVNGSNAPKRMNIGWCIGSAGASLIELLIAMAISSIAISASIHV
                     FSSVGLRFTAQHSTMVTNQDLRLGLDVLCSEVRLAGAGLLGEDSPFLKAKSDEIEFFA
                     NLSGVSTTLTQVAEIGRQDLPVEEGAGWPKGKQLLVCTAVHCAWNQLAADGRKQALTL
                     VTPTAEQFPVRSAIFLLNRVRYYVKRQDDGAMRVMRDVDGGASTLLGDVSQFELQYFD
                     REGRVTTDLSEVVRVRVAIQAGRQGSRLVRDVAIRM"
     gene            13059..14534
                     /locus_tag="PK614_01825"
     CDS             13059..14534
                     /locus_tag="PK614_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80983.1"
                     /translation="MGRSRIAVRRDERGMVLLAVLMVVFLLTLLGMTSMQLAGQEIVG
                     ASALQEERLAHHAAEAAVDVVMGWFHDPALHPQGVEMTWLTKRLVNAQGDASYFDAQG
                     RAQFAGTGASPDVVFDAAHAQHDRLMNDPQTGWFKSLKGLARILKLRVYGATRPGLLC
                     TVEVTAGAGHTSRVTKTVSVEFGAYAVPALHAPIQGGTVGNESAPSSAGSVFAHWGDM
                     VVRGKAYFPRPEAVPMKSGLAPVTGQSYDEMTHREDRWFTIRLGGDAFFAQLPAEAWS
                     GVPLNLHMHQDPVPGLKVDQWDYDTLKRMAKQFGQYYGIDRDGLLYVGGVIQPGLGRP
                     ASEVFASKGPGDHRGLIFVDTLDGTSPRPDNLGTIVLDQEYAEGIFIVNAHVLWKTGA
                     YGKSVSALSPPPEGQQSLGARIPVQLSGIHLQGVLYVAGDVRYAGHLKVYGGVVAQGA
                     IVDGTNGSGMLEAWYNHDLRDGLVQGMPLVFVAPGSWQAKI"
     gene            14639..16438
                     /locus_tag="PK614_01830"
     CDS             14639..16438
                     /locus_tag="PK614_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRB80984.1"
                     /translation="MSRATVSMLPVSECSQAVSPLAQTRSAACYHRLVEQGFLQQEEL
                     VLAMAEAARKRLDVATVLMERYRIPKPALGAALAEFYHCPFLAYDERTVMERELLKNL
                     SLDYLRMNHWVPLRRQGNGIEVLIDDPHNPDKLFDVRRAFPGQALSYRVGLRCDIARL
                     LNAIQGRESGDAISDILGELVSEAQLEEQQNASIAAITENDSAIVRLANQIITDAYRR
                     GASDIHIEPYADRKETAVRFRVDGTCFTYMKIPAAYRRAIVSRLKIMASLDIAERRKP
                     QDGKIRFKLGTGQEIELRVATLPTSGFNEDVVIRLLTAHGARRLEDLEFSDGTRRLVG
                     QLAQKPHGIVLCAGPTGSGKTTTLHAILASINTDERKIWTAEDPIEITQDGLRQVQVH
                     PKIGLTFATTMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVFSTIHTNSAVET
                     VVRLLDLGCDPFNFSDAMLGVLAMRLCKRICPNCREAYHPTRSEYDELVQAFGEGDWE
                     GVHPAYNPGLTLFRGRGCDTCNQTGYRGRVPIHELMVVSDSMKALIQARARTGELLTL
                     AKSDGMKTLVQDGIEKVLQGLTTYKQVRAVAIK"
     gene            16817..16936
                     /locus_tag="PK614_01835"
                     /pseudo
     CDS             16817..16936
                     /locus_tag="PK614_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            17301..17825
                     /locus_tag="PK614_01840"
     CDS             17301..17825
                     /locus_tag="PK614_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB80985.1"
                     /translation="MKHRQNSHPCFSLLAAAQRGFTLLEAMIAAGVLSVGLLGLAGLS
                     GMSLGKNVDANDMSRVTNIAADMAERIQNNRQRVLDYHNTNTSVACAQSANTQRMALG
                     DCTQWQALVANSGLTGATGLVTAARLDPDPTANPVTMNRFLVTVTVSWQTRQTDVSTA
                     RTKTAIFTTVVAPE"
     gene            17846..18949
                     /locus_tag="PK614_01845"
     CDS             17846..18949
                     /locus_tag="PK614_01845"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HRB80986.1"
                     /translation="MRRFDWNQQGFTLVELMAAVLITVVIVAATMTTVVTSNRANVVN
                     TQVADTQQNVRLAIDLLSRDIKLAGFNYNATDPATGSVGACNATIGAVVKPVGLLPQD
                     QTPTGADTGADSVSMVLPVMNTTGWILTAAVGGTPNNKTYDNSISLSGAAMTEMVAQG
                     LVVGSTISIGGALSKTVQAVNATSIGFGTGNFVDGQFPVGTPVYLMQCVRYQVVVNTP
                     ATCGSDTPCLVRNNVPLVDGVEDLQLAYACDGCNQAAPNPLFPDGVVDDQDASSTSGF
                     PTFTQGDFVSNSAWAIAPWTPDKIRLAQVSLVVRPTNADDGLDEKGTKAVNTTGPVII
                     GDHNPSADTGYNASTYMQQRRRVMIRTIQPRNL"
     gene            18964..19596
                     /locus_tag="PK614_01850"
     CDS             18964..19596
                     /locus_tag="PK614_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HRB80987.1"
                     /translation="MNLRQATTGRGQQDKCTGLKNQRGIALLTVMLMLLILTILGIAS
                     ITVTSMENRMAGFFRTTEAVVAAADSCEGLAANIIQQTLSPPGVLPASFIAPTGPVPT
                     ANATTLYAEIYGYDLPSPAPANTPAVNYVDVASTAPNFVMTNLPGFTVNGDIDLLYTH
                     TKSGQGTGVAGTEHVYRITCMASNPATGSSSTVTSVYGCLDGDTCVKKIN"
     gene            19613..19942
                     /locus_tag="PK614_01855"
     CDS             19613..19942
                     /locus_tag="PK614_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80988.1"
                     /translation="MNNTVCIVMLGLLTLGQTMPSVPSSSSAETEQAPEAMRFSTAGF
                     QAGVIDEVQGSSIRIDGRSYVLKTAVVVVTHEGEPLELERIIPTSLVKFHLKEGHIDK
                     MVVTLPQ"
     gene            20020..24483
                     /locus_tag="PK614_01860"
     CDS             20020..24483
                     /locus_tag="PK614_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB80989.1"
                     /translation="MNRRTLVAVMLVAGSLSAALFGSRDAVAVVSNADYTAVPAFVSN
                     TTMPNILIVMDNSGSMSNRACESSSCGVLPDGSTSTVTTFNATTRYNGFADPLRCYVW
                     DATDNRFENGAVKVTLNTSCTNEWDGNFINWATFRRFDAVKKAMTGGNCHHPTASPVR
                     NPDGTCKPYGSPALPTVKAQNLGTSDETTPAIPYAGGIGDLTYVGRIPTTVHTGDPAN
                     LYVRYEADGDMCIDNDNTGDGCPDSGGFAETELNEIAFAMYSEPTGVIQQIGAKARFG
                     LAVFNGSSTNDGLRVLTPIASRQSINFSNSTVETFNTNTAAMIDSVDESTPATWTPLA
                     ETLYDAIRYVAQINSVYYPTEYVYPIAFSSSSAVGFGSSGIGSIGTSEISALTGSEAC
                     PAGYISGGTTATCAAGRDPFFFGSNHTPAWASTSQVVSCCKTFVIVFTDGEPTQDRNI
                     PAALRDNPHNGVDCTGTDNLAPPRPVDGTCNNHPSTPVTDLLGEHKTTYADNGSGYLD
                     SVAYWAHTTDLRPCNGVADGTIAVIMETGHCLPGTQNVSVYTFFAFGNIAGRGILAQT
                     ARLGAFDDSNNDGIPQTNEWDKENNYTGSSTPDGIPDAYFESANVDDLQDKLLATIAS
                     ILRRSASGSSVSVLATASTGEGALYQSYFYPSLDETATHNDVKWTGYTQALFIDTFGN
                     TREDTNQDGRLDYKVDKIIKTRFDAFLNLVKVDKYVDSDGDGLPNDQNTDGQVTVADC
                     NPCGQALSDIVPIWEAGKQLALKDASTRNILTWVDSDHDGVVDSAEQIAFTTANEPTL
                     RPYLRAASSAEGTKIINFVRGCDATTCAEQATTRDRRLSVTNGGSTSLKVWKLGDVIH
                     STPTVVAGPKDRHDAIYGDQSYSAFLQKWYNRRQVAYVGGNDGMLHAFNAGYYHPGDD
                     SSTSYNPTTGGIEHGWFTKAPADNSTGAPLGDELWGFVPYELLPQLEFLSRADYQHTY
                     YVDLPLKVADVRIFTPDTDHPNGWGTILIGGFRMGGSCGACSAGTGAPPMTVNISGTD
                     YTFYSSYFAMDITNPDAPKLLWSFSSSNLGLTTSVPAVVRVNPAADPKASNTNAKWYM
                     VVGSGPTGYDASVVQSAKVFAVDLATGPGSGNSLVTSYSVGSWNSYIGDITAFDRDLD
                     YRHDVVYFGRVIHDGALPWRGKIYRLTMGAGGATAKFGTVTSPTQWGIASGSARVPTE
                     MLDTFVSGAEMGPVATAPSVTTDDAANVWVFAGSGRYYSKSDKTDVSTQYFVGLKDSV
                     LNSGCNQSAGALNCMDNNLVDVSNATVCVVGVGDCGQASGTNQVSGVSGATTFTGLIG
                     LVQSKDGWVTKLVEPANPPTNPVPYGIGERAVSSPTVFGGVVFFPTFIPTNDICVSSG
                     TSRLWALFYKTGSAYQEPIIGTTTSGANQTVNKFGSLGEGLAFGIVVHMGSGRDGGSP
                     FGLLINMSQGNFGDCATCGFGGGSPGGLPSSNIGANVAIDPRSRYFSWTNM"
BASE COUNT         5047 a         6439 c         7709 g         5317 t
ORIGIN      
        1 atgatgcacc aggccaccgt cattcaggtc gaaggaaatt ttgaccaggc gctgaccatc
       61 gtcaaagagc tgtcggccac gtaccccgtt gagttggtca attctctgaa ccccttccgg
      121 atcgaggggc agaaaacggc cgcgatggag atttgtgacg atctcggtga tgcaccggct
      181 atgcatgtac ttcctgtagg gaatgcaggt aatatcaccg cctattggaa cggctatcgg
      241 gagtatcgtg cggccaatca gacgacgagg ttgcctcgga tgatgggatt tcaggccgct
      301 ggtgcggcgc cgattgtctt gggccatatc gtcgaagagc cacagacggt ggccacggcg
      361 atccgaatcg gcaacccggc cagttggcaa tcggctctca ctgcggtgaa ggagtcgtcc
      421 ggtgccattg atatggtcac cgacgaagaa attctgcatg cctatgcgtt ggtggccagt
      481 gaggaaggtg tcttttgcga accagcatcc gctacctccg tggccggtgt cattaaattg
      541 agtcaggccg gacaattaaa agaaggtgcc acggttgtgt gtacgttgac cgggcatggt
      601 ttgaaggatg ccgatacggc gatcggcatt tcacgtcagc ctcaaacggt gaaagccacc
      661 cgcgatgatg tggcccgcct ccttcatgtc tgattacctc aggaacattg catgaaatat
      721 ttgatcctgc atgccgacgg gatggcagat ctcgcgtgtc cggaactcgg tggaaaaacc
      781 ccgcttcaag cggccgcaac tccgaacctt gatcagatcg cccagcgtgg cgaggtcggg
      841 ctgttgagcc ttccctcgga agccggagcc gccggaagtg atgtcaccag tgtggctgtg
      901 ctgggatatg atccgaaaaa gtattatccg ggccccggtc cgctggaagc ggccggtctc
      961 ggcgtcacgg tgggtgagca ggacgtggtg ttccggtgca ccatggtgac ggtgcggggg
     1021 gagtctgctt ccggtagcaa agatcgggca accgacatca agaaactcgg gccgcatgtc
     1081 ctcatggagg acgcgaccgc cggtctcatc gacacggagc aggctcgtga gttgctcgaa
     1141 gcggtgaatg agcagcttgg ctcggaatcg attcagttct accccggttc cgggcatcga
     1201 cacctcatgg tctgggtcgg aggaaaatcc cgcgcgacgt gtctcgatcc acaaccgttg
     1261 gtcggccggt cgattgccga tgcgttgccg agcggtgatg gcgccgacgt gctgcgcaag
     1321 atcatggatg cggcgttttt catcctgcgc gaccatccgg tgaatgagga acgtgagcag
     1381 gaggggttga agcccgccaa ttgcctgtgg ttgtggggcc agggccgtgc cgccgtgtgg
     1441 ccgcctttgc cggagcgata ccagattgcc ggtgtggtgg tgtcatccag tgatgtccat
     1501 cgtggagttg gggtgtgcgc cgggttggat gcggtcgagc ttccgcttgc cgacggaagc
     1561 gacgcgaatg agttcacggg ttgcgtgcag gcggcggtgc aggagttggc caagaaggac
     1621 ttcgtctatc tgcatgccgg gctttccgac gacgtgctgc acgcgactga tgtgcaggac
     1681 aaggtgcggg ccattgagcg gttcgatgcc caggtcgtcg ggccgattct cgcggcactg
     1741 gcgacgcatc cagcctatcg gttactggtc gtgtgtgatc ccggtctcgc aaagagccac
     1801 ggtgctgagg ttccgccgat cctgtatgcg ctgtgtgata gtgccgccaa gccgtcggcc
     1861 ggagtcacca agcggtttca cgagcaggat gcgaacatcg ccgggaccgc gcctcgcgat
     1921 gcgacgaagt tgatgctgcg attattcccg cgcggagtat agagctgtgg cgctgatcgt
     1981 tcagaaatac gggggaacat ccgtcggcac ggtcgaacgg attcatcgcg tcgccgaacg
     2041 tgtcgaacgc gctcagaaag acggccacca ggtggtagtg gtgttgtcgg ccatgagcgg
     2101 ggaaaccgat cgactgctga agttggcgca tgaggtcacc ggtgcaccgg acgagcggga
     2161 attggacatg ctgctctcga caggggagcg cgtgactatt gcgttgctcg ccatggaact
     2221 ccgggggcga ggggtgaatg cccgctcctt cacgggacgg caggtcggca ttcacacgga
     2281 cagtgcgcac acgaaagcta gaatttcacg cgtcaaggcc gatcgaatca aggaagcgtt
     2341 gtctgccggc gtgatccccg ttgtggcggg attccagggc attaacgcca gctcggatgt
     2401 cacgacgctg ggccggggag gttccgatct aacagccgtt gcgctggcgg ctgcgctcaa
     2461 ggccgaccgc tgcattattt atacggatgt ggatggtgtt tacactgccg atccgaacat
     2521 cgtgccggct gcgcgacgtc tggataagat ctcctacgaa gagatgttgg aaatggccag
     2581 cctcggcgct aaggtgctgc agagtcgttc ggtggagttt gcggccaagt actccgtgcc
     2641 ggtggaggtc aattcaagtt tcaaagaagg aaagggaacg ctcgtgacac gtgaagatgc
     2701 cgatatggaa ggggtcatgg tgtcgggtgt gacgggggac cgcaaccagg ccaagattac
     2761 gattgtcggt gtgccggatc ggccagggat cgccgcgcgt gtcttcgggg ccgtggccaa
     2821 tgccaacatc gtggtggata tgatcatcca aaacgtcagc caggcctcca caaccgacat
     2881 ttcgttcacg gtccccaagg ctgatttgcg taatgccgtc gatctcgtcc agcgtctgtc
     2941 cgaagagatc ggcgcccggt cggtggctgt gacggaatcc atcgccaagg tgtcgctcat
     3001 cggggtgggt atgcggtcgc attccggagt ggcggcaaag atgttcgagg tgctctcgcg
     3061 cgaaggcgtg aacatcatga tgatcagcac gtcggaaatt aagatttcct gtgtcatcga
     3121 ggaaaagtat ttggaattgg ccatgcgtac gcttcacacg gcgttcggcc tggatcgggt
     3181 ttcggcacct gccctgggct agtctgctcg gagggcggtc acggtcggtg tgccgtctcc
     3241 tcgacaggtc cgtcatgacc ctgctactct cacatctatg aaacgtcgca acacagcgcc
     3301 gcggcgccct cgcggttcgg aacctgtttc ggtgccgccg ctcacggcgg cgcaggcgtc
     3361 cgcgctcgaa atctacgata ccaccttgcg ggatggggct caggcggagg atgtcagttt
     3421 ctccgtggag gataaactcc gcgtggcgca acggctggat gaactcggtg tgcatttcat
     3481 tgagggtggg tggcctggcg cgaatccgaa ggacatcgag ttctttcgca cggtcaagac
     3541 cattcccttt aagaatgcga ccatcgtggc gttcgggtcg acgcgcaaag ccagcaactc
     3601 ggttcgaaaa gataaaaacc ttcaagaact gctcgcatcc ggaactccca ccattacgct
     3661 cttcggcaag agttggtcgt ttcaagtgac ggatgcactg ggcattgccc tcgccaagaa
     3721 tttggagttg atcgaagact cgattcacta cctgcgctcc aaggatcgtc gggtgtttta
     3781 cgatgccgag cattttttcg acgggtataa ggccaatccc gactatgcgt tgcagacgat
     3841 tcgcagggcg attgccgggg gagccgagcg ggtcattctc tgtgatacca acggcgggac
     3901 catgccgtgg gaaatccgtg aaatttgccg cattgtgaag caggaatgtc ccgtgccatt
     3961 ggggattcat gcgcacaacg acagcgaaat ggcggtggcg aactcgctcg tcgccgtgga
     4021 gtcgggcatt ctgcaggtgc aggggacgat caacggcatc ggtgagcgat gcggcaatgc
     4081 caatctctgc tccatcatcc cgaatctaca gttgaaaatg cagcggcctg cgctcggcaa
     4141 taagctgacg cacctgaagg atgtatccgg gttcgtgacg gagatcgcca acctgatgcc
     4201 caataagcgg caaccgtatg tcggtgatgc ggcgtttgcg cacaagggcg gcgtgcatat
     4261 tcatgcggtg ctcaagaatg cggcgaccta cgaacacatc gatcccgcag cggtcggaaa
     4321 ccgtcgtcgg atcttggtgt cggattatgc ggggcgcagc gggctcctgg aaaaggttga
     4381 agcgtacggc atttcactga acaagaatca cgcaaagttg caggaactgg tcgagacact
     4441 caaggagcgt gaaagccaag ggtatcaatt cgaaggcgcg gaaggatcgt ttgagttgct
     4501 gatgcgcaaa gcgatgggca gccatcgccc gtcttttcag ctgcttggtt tccgggtgat
     4561 cgtcgagaag aagcaggagc acagtctgct gctgtcggaa gccaccgtga tggtgaaggt
     4621 cggggaggtc gtcgagcaca cggccgccgt cggggccggc cctgtgaatg ccctcgacca
     4681 tgcgctccgc aaagccttgg aaaaattcta tccgcaactt cgggaagtga agctcctcga
     4741 ctataaggtc cgtgtgctct ctgcggataa agggacggaa tcaaaggtgc gcgtcctgat
     4801 cgagtcgggc gacaacaagg acaagtgggg caccgtcggt gtgtcggaaa acattatgga
     4861 agcgagttgg caggcgttgg cggacagcat tgagtataaa ttgctgctgg cggatcgcta
     4921 ggctcttttc acctccgtcg ggcgcacata atccttgaca gggcaggtaa cctcacgtaa
     4981 cttatgggga aattctttgt cttggacgct cgacaccaag aagcacttcc taaggaacga
     5041 ctctagtgga tggcgggggg aaaggcatga gaaaggcgga tatcgcgaac gaaatcttca
     5101 agcaggttgg agtctccaag aacgatgcag ccgatatcgt cgaactcgtg ctcaatctgc
     5161 tgaagggagt gctgcaaaaa ggtgacgcgg tcaaaattgc aggattcggg aattttgtag
     5221 tacgaagcaa aggggcccgc aagggacgga acccgcgaac cggagaagaa atcgggatta
     5281 ctccccgtcg agtggtcacg tttcggccca gtcaggtgtt taaaaagtac gtcaattcct
     5341 agtcgctacc ttgttacgag cacaagcagc atgaggaccg gtcatgggga atgagccccg
     5401 actgggaagc aaagtttttt acaaaatcgg cgaagtcagc aagatttcca aactgccggc
     5461 ttatgtcttg cggttttggg aatcggagtt tagcttcctg agaccgaaga agagccgtgg
     5521 gaatcagcgg ctctatgtgc agaaagatgt agatacggta ctggagatca agcggatgct
     5581 ctatgacgag gggcatacgc tggccggagt taagcgctac tgggctcgtc gaggacgagt
     5641 taccgtgaag aagctgagct cgcggaaaga actggcacaa cgtgtcaggg gcgatctcca
     5701 ggcgatcatc agaatgatcg attcccattc gtcctgaact gttcttgtga tgcgggcggc
     5761 tgcgtccaat tgtgggtaca tcgatgtcgt gtgtgtcggg gcgtagcgca gcccggtagc
     5821 gcactcgctt ggggtgcgag gggtcgtggg ttcaaatccc gccgccccga ccatcggcac
     5881 gatgttctct accactgtca gtgtcctgag ggacgttgat cattgcggac gatcttgtga
     5941 gcttgttcga gtgcgttcct gcctctttcg tccccactct tgatccagcg gtttcggtgc
     6001 tcccctttca cgacgtgtgc gaagcataaa ggagcggttg tggcgcgtgt acggatcctt
     6061 gtgaccaatg acgatggaat tacgtctccc gggattcacg cggtcgcggc ggcgttgggt
     6121 gcattggggg aggtgtggat tgtcgcaccc gatcgggagc gtaccgcagt cgggcatgca
     6181 gtcacgttgc ataaacccct tcgtattacc aagatggcgc ctcgcgtatt tatggtgaac
     6241 gggacgcctg tggactgtgt caacttggct ctggtgaaag tcttgcccgc gcggccgtcg
     6301 ctgattgtgt ccgggattaa tcgaggggtg aacctcggcg acgatgtgat gtattccggc
     6361 accgtgtcgg gggctctgga aggaacgatc ctaggcattc cttcgatagc cgtgtctcag
     6421 gaaggcaacg agacatttcg attcgatgtc ggagcgcagt atgcggctcg agtggctgcc
     6481 gaggtattgc ggcatggatt gccgccggaa acgatcctga acgtgaatat tccgaatgtt
     6541 ccggtccggt cgatcaaggg tgtgaaagtg acctgtttga gtcggcgtcg tttcaacaac
     6601 cccatcgttg aaaaagtcga tccgaggggt cggaagtatt actggatcgc cgggacgcgt
     6661 cagtcgtgga gccgcgagaa cgatgccgac cacgaagcgc tggagcagcg catggtgtcg
     6721 gtgaccccga ttcatctcga cacgacccat catgaggtgc tggaacagtt caaggcgtgg
     6781 gagcaaccgc tctcgcgacc atccgcgcgt ccgaaaacgg tgaagccgca tcctcgaagg
     6841 agagcccagg cgtgaccgga ctcatcgagt ggttgataga agtgctcggc cggttcgtga
     6901 tcgccacgat ttcgacgttc ggctatacgg gtattgtcat tacgatggcg atcgaaagcg
     6961 cctgtatccc gctgccgagt gaaatcatca tgccgttttc cggctacctg gtctccaccg
     7021 gccaattctc gatgatgggc gtgacccttg ccggcgccat cgggaacgtg ctggggtcgt
     7081 tggtggccta ctacgtgggg gtgtggggag ggcggccgtt tgtcgagcgc tatggccgat
     7141 atgttctggt gtcgcagcat gatatggaac tggccgatcg gtggtttgcg aaacatggag
     7201 aggcggcggt gttgatcggc cgtctactgc ccgtggttcg aacgttcatc tctctcccgg
     7261 cggggattgc gcgcatggac gtcaagaagt tcgtgctcta ttcatttgtc ggagcggttc
     7321 ccttctgtta tgcgttggcc tatgtgggct tgaagatggg ggagcactgg aatcaattac
     7381 gtcagtattt ccatcacgcg gatctggtga tcgggttgtg tctcgccgtg ggcctcgggt
     7441 attttctctg gtctcactgg ccgaagcgtc gtccgtctat ggagtaattg cgcgtcatgt
     7501 tgcgtgtcca caataccctg accggaagca aagagccgtt cgaacccttg gtgcccggca
     7561 aggtccgcat gtatgtgtgc ggggtgacgg tgtacgacta ttgtcacatc ggacatgccc
     7621 ggagtgcgct ggtgttcgac gtgttgcgcc ggtatctgga atattcaggt ttcgtcgtcg
     7681 agtttgccaa gaacttcacg gatgtcgatg acaagatcat caagcgggcg aatgaacagg
     7741 gcgtgagctg cgagcacgtc accaccacct acatcaacgc ctattacgag gatatggaga
     7801 agctcggtgt gcgccgcgcg acgctcgaac ccagagcgac ggaacatatc gccgacattg
     7861 tgaggcttgt cgacaggttg ctcgccaagg gaatggcgta tcgtgtcgac ggggatgtct
     7921 atttccaggt cgatcggtat ccggtgtacg gccggctctc gaagcggaac gtcgatgatt
     7981 tgcaggcagg agcacgggtg gatgtcgatg agcggaaacg gcacccgatg gatttcgcac
     8041 tctggaaagg cagcaagccc ggtgagcctt cctgggatag cccctggggc ccgggtcgtc
     8101 ccggctggca tatcgaatgt tccgcgatgg ccatgcggca tcttggtgaa accttcgata
     8161 tccatggcgg ggggatggat ctcatttttc cgcaccacga aaatgagatc gcgcaatcct
     8221 gcggggccac gggcaaggag ttcgcgcggt actgggtgca caacgggttc gtgcagatca
     8281 atcaagagaa gatgtccaag tcgctgggca acttttttac tattcgcgaa atctttcaga
     8341 agtcggaatg gtccgacacg gtgaccggcg agatgctgcg ctatttcctc ctttctacgc
     8401 actatcgaag ccccttggat ttttccgatc agagtctgaa cgaagcgaaa aacgcactca
     8461 acggatttta cgatctcttt gaacggctga atgaatcggc cccagcccat ggtgcggccg
     8521 atcagcagat gcaggagtcc acgatgcggg cgcgagcggc attcgtggcc gccatggacg
     8581 atgatctgaa tacgcccaat gcggtggccg ccttacagac gttacgtggg gaagcgaaca
     8641 aggctctcga ggtcggattg tccggtgaga tgcgccgggt ggtgcggcaa gagttccgcg
     8701 tcttagggat cgtcctggga ctccttcaga ccgacacctg gcgatttaaa agtcagcggc
     8761 agcagccatc atccggcgga gcggaggcat ccacggacac cttgtcagac gaggacattg
     8821 ccgacaagct agccgcacgt ctcgccgcaa agcgatcgaa gaattaccaa ctcgccgacc
     8881 agttacgagc agagttggcc tcccatggca ttacgattga ggaccggccg gatggcacca
     8941 gtcgatggaa acgataactc gctggaggtg atttacggcc tccacgccgt gcgcgaagcg
     9001 cttcgcgccg gggttcgtcc gatccaacgt ctcctcgtgt tgggaaccga ccgacagttc
     9061 ggggaaatcg cgcgtctggc cagggatcag cgtgtgcccg tgcgagtgga accgcgggct
     9121 gcattggatc gcctggttcc ttccggtcac caccaggggg tggtgggtgt ggtcgctgcc
     9181 aaagcctatg cggagccgga cgatattctc gcttccgcac aggctgaagg gcagacgcca
     9241 ctgctggtcc tgttggacgg tgtggaagac ccgcacaacc tcggggcgat tctgcgaacg
     9301 gccgaggcgt cgggtgtgca aggcgtcttt attccggagc gccgcgcagt ggggctgacg
     9361 agtgtcgtgg caaaggcctc tgccggggct gtcgattaca tgcccgtcgg gcgcgtgccg
     9421 aatctgtcgc gcttgatcga acgtcttcaa ggcgcaggca tctgggtcta tgcgttggat
     9481 gctgaggctc cgaagcccta tactgcgctg gatttcagcg gcccggtagc gctcgtcttc
     9541 ggcggggaag ggacgggggt tcgcccaggg gtgcgtgacg cctgcgatga ggccgcccac
     9601 attcccatga tggggaacgt cagctcgctc aatgtctcgg catcagcagc catcgtgctg
     9661 tatgaggcat tgcgtcaacg tcgggaaggt gcggggaaga agccgtaaga aaggatgcga
     9721 agggctcacc ggagcttcag cattttccct tggatggcgg ccttgagcaa ttgggcggta
     9781 ttggagaccc ggatcttttt catcatattc gcccgatgcg cctcgactgt cttgacgctg
     9841 atctttaacc gctgagcaat ttctttgttc ttgaatccgg tccagatcaa ctcaaggatt
     9901 tcctgctcgc gggttgtgag ggcttcggga cgtcgtttgc gagggggaag gaccggatcc
     9961 ggtgcggata ctttggctcg acttttcgtg gtcttggcga ctggcgacat gtacatctag
    10021 ctccacttca ggtaaaggca attcactgat tgaggtttgc gatcttccgc gccaaaattg
    10081 ctcccggatt atacgcagtg cccccggcgt ttgtaaatga aaagcgccaa gacgaggggg
    10141 cctcgtgcct gctcctattg ccctaggatt aaatgcgtat acttaggagg gggatcatgt
    10201 cgagcctggc cacattgttt caggaatgtt catgatttca tatgttacgg tgctgctatt
    10261 cggcgcggtg attggcagtt ttttgaatgt ctgtatctac cgcctgccgc gtgaagagtc
    10321 ggttgcctgg ccggcctctc actgtccctc ctgtcgccag gccattcctt tctacgacaa
    10381 cattccgatt gtgagttatc tgcttctgcg gggccgctgc cgggcctgcc atgcgccgat
    10441 tgcgatccag tacccagtgg tggaagcggc caacgcgatc ggatacgtgc tggtcttttg
    10501 ggcgtttggg tttacgccgg cggcctgggc ctatgcggcg ctggtgtctg cgcttatcgt
    10561 ggtgacgggg accgacgttt cccatacgat gatcccggac gccgttacgc tgccgggaat
    10621 cgtcatcggt ctgctgtgtg ccatcttgat cctcccgatc ggacttgtcg attccttgct
    10681 cggtgtgttg ttgggcggcg ggatcttatg gtttttagca tggatcagtc cctatgtttt
    10741 cgggaaggaa gggatggggg gcggggatat caagctgatg gccatggtcg gggccttcct
    10801 cggctggcag ccggtgttgt tggcgattat gatcggatct tttctggggt ccattgtcgg
    10861 tgtcgggctc atcgccatcg gggtcatgcg gcgtgagcag tacattccat tcggaccgtt
    10921 cttggcagtg ggatcaatcc ttgccctgtt gtttcatcag cccctgcttg agtggtattg
    10981 ggcgctgatc gacattccgc agtaaccgcc ttcaaatcgt tatccagaga cagtttccaa
    11041 ggatcagcgc ctggccgaac tgtatctgaa cggatgattg actgtatccg attcggaaca
    11101 gttcataact gttcacgcgc ttttccctgt cctacctcag gcaacattcc acggctcacg
    11161 gcagatttcc tcaacatttc tatagcttcg ccggccgctt cgtagaagag gcggcctcat
    11221 tgtttctacc ggcgtcggga atgagaaacg gcatggaact tggaaatgct ctacgcgctc
    11281 gaagaaagga gccggtatga acgaacgtgg cgggagtctt gtggagctgt gtacggtagt
    11341 cgcgatcatc gggattgtgg tgggcgtgag tgtgcctgga tgggccgccc ttgtcgcaaa
    11401 acatcaacag cgcgcggtga tggtggagat cgcatcggag ttgcgcatgg cgcggcaatt
    11461 ggcgatggcg aggcatgagc gtattcgagt ggtcgtggat gcggaacagt ctgaattgcg
    11521 cacggaatgc atggactgtg acacaagcct gctgcgccgg tatgagttcg ctcacaaagg
    11581 cacggcggtt gagtcgatga cgacgaaacc ggaaatcatg tttcagccca gcggtcgttc
    11641 ggccacggcg accaccatgg tcctggtcga cggtcgtcgt gcgacacatc tggtcacggt
    11701 gagcatcacc ggacgggtga ccctgtcatg acccaatgtg agccgaatct tgtgacgcga
    11761 cgatctgcgt tgatgagtgc caaagggttc accctcgtgg agagcatggt ggcgttggtg
    11821 gtgctgtcga tcggcgtgat cggaacgatc ggcatgtgcg aatgggctga gcgcggtctg
    11881 cagcgcgggg cgttgacgac aaccgcgctg gcgctcgctg aatcacgcct ggaggcgaag
    11941 cggagtgtgg cgtgggagcg gttgttgatg gacgacgtgg atcaagacgg aatagtggag
    12001 tccgtcatgc gcgacgatgg cttgcaggat gatcaggcga acggcgacgg catcttcacg
    12061 gccagtgcca cccagtccaa catccggctt gtctggaccg tggaactgaa tcgcggccac
    12121 gagccggcca gggcgagcct ggcgactatc gaggcgcgtg caatcttccg gactatggga
    12181 gggcaggaga aagaggttcg cgttcgcacg atccgggcca atcctcggta tgtcggcctg
    12241 tctgtgttgt catgaggcgc ggcatggtca acggttcgaa cgcgccgaag cgcatgaata
    12301 ttggatggtg catcggctct gccggagcga gtctcataga actcctcatc gcaatggcga
    12361 tcagcagtat cgcaatatcc gcttcgattc atgtcttttc ctcagtcggc ttgcggttca
    12421 ccgcgcagca ctcgaccatg gtgacgaatc aggacctgcg cctcggactg gatgtgctgt
    12481 gcagcgaagt acgtctggct ggagccgggt tgctaggtga agattcgccg tttctcaagg
    12541 cgaagtcgga cgagatcgaa ttcttcgcca acttgagtgg tgtgtcgact acgctgacac
    12601 aggtcgcaga aatcggccgc caggatctcc ccgtcgaaga aggagccggt tggccaaaag
    12661 gcaagcagct actggtgtgc acagccgtgc attgcgcctg gaatcagttg gctgccgatg
    12721 gacggaagca ggcgctgaca ttggtgacac cgactgccga acagtttcct gtgcgtagcg
    12781 ccatattcct gctgaatcga gtgcgttact acgtgaagcg ccaggatgat ggggcgatgc
    12841 gggtcatgcg tgatgtggat ggcggtgcga gtacgctctt gggcgatgtg agtcagttcg
    12901 agctgcaata ttttgatagg gaggggcgtg tgacgacgga cttgagcgag gtggtgcggg
    12961 tacgtgtggc gatacaggcc ggacgacaag gatcgaggct ggtgcgggac gtggcaatcc
    13021 ggatgtaata ggtaaaagga ataactatga agacaagcat gggacgatca cgaattgcgg
    13081 tgcggcgaga tgagagggga atggtgttgt tggcggtgct catggtggtg tttctgctga
    13141 ccttgctggg gatgacttct atgcagttgg ccggccagga gatcgtcggc gccagtgcgt
    13201 tacaagagga acggcttgcg caccacgctg ctgaagcggc ggttgatgtg gtgatgggat
    13261 ggtttcacga tcctgcgctg catccgcagg gagtcgagat gacgtggttg acgaaacggc
    13321 tggtgaatgc acagggagat gcctcctatt tcgatgcaca ggggcgcgca cagtttgcgg
    13381 gaaccggtgc gtctccggat gtggtcttcg atgcggccca tgctcagcac gaccggctta
    13441 tgaacgatcc tcaaacgggt tggttcaaat ccctcaaggg gcttgcgagg attctcaagt
    13501 tacgagtcta tggggcgacg cggccgggac tgctctgcac ggtggaggtg acggccggag
    13561 ccggccatac ctcacgggta acgaaaacag tatcagtcga attcggcgcc tatgctgttc
    13621 cggcgctgca cgcgccgatc cagggcggca cagtgggaaa tgagtccgcg ccatcctcag
    13681 ccggttccgt gttcgcccat tggggcgaca tggtggtacg tggcaaggcg tatttccctc
    13741 ggccggaggc ggtcccgatg aagagcgggt tggcgccggt gacggggcag tcctatgacg
    13801 aaatgacgca ccgcgaggat cgctggttca ccatccgact cggtggcgac gccttttttg
    13861 cgcagcttcc ggcagaggcc tggtcgggag ttccactgaa tctccacatg caccaggatc
    13921 cggtgcccgg tcttaaggta gaccagtggg attacgacac cttgaagcga atggccaagc
    13981 agttcgggca gtactacggc atagaccggg atggattgct atatgtcggc ggtgtgatcc
    14041 agccaggatt ggggcgccca gcctcggagg tgtttgcctc gaagggcccc ggtgaccatc
    14101 gggggttgat ctttgtcgat acgctcgatg ggacttcccc gcgacctgac aatttgggga
    14161 cgatcgtgct tgatcaagag tatgccgagg gcatcttcat tgtgaatgcg catgtgctct
    14221 ggaagacagg ggcgtatggg aaatcggttt cagctctcag tccgccgccg gaaggtcagc
    14281 aatctcttgg agccaggatt cctgttcaac tgtccggtat tcatctgcag ggtgtcctgt
    14341 atgtcgcggg tgacgtgcgg tatgcagggc atctcaaagt ctacggtggg gtggtggcgc
    14401 aaggagcgat tgtggacggc acgaacgggt cggggatgtt ggaagcttgg tacaaccacg
    14461 acctccgcga tgggctggtg cagggcatgc cgctggtgtt tgtggcccct ggaagctggc
    14521 aggccaaaat ttgacggcag cgctacgagg ttgacgcggt cactctgcca gcatgttgag
    14581 ttcagacaat catctacaat cgaatcgtct ccacggtatt cagaaaggaa tcggtcccat
    14641 gagtcgtgcc acggtgtcca tgttaccagt cagtgagtgt tcccaagcgg tcagcccttt
    14701 ggcgcagacg cgctccgctg cttgctacca ccggctcgtc gaacagggat ttctacaaca
    14761 ggaggagtta gtgctcgcca tggctgaagc cgcgcgaaag cggctcgatg tggctactgt
    14821 gctcatggag cggtatcgga taccgaagcc ggcgctcggt gccgcactgg cagaattcta
    14881 tcactgcccg tttctcgcct atgacgagcg cacggtcatg gagcgagagt tattaaagaa
    14941 tctcagcttg gattatcttc ggatgaacca ttgggttccg ctcaggcgtc agggcaacgg
    15001 aatcgaagtc ctgatcgacg acccgcacaa tccggacaag ctgttcgatg tgcggcgagc
    15061 gtttccaggc caggcactgt cctatcgtgt cggactccgt tgcgatattg cgcggttgtt
    15121 gaatgccatt caagggcgcg agtcgggcga tgcgatatcc gacattcttg gcgagttggt
    15181 cagtgaagcg cagctcgaag aacaacaaaa tgcgtcgatt gccgcgatca cggaaaacga
    15241 ttcggccatc gtccggctcg ccaatcagat cattaccgac gcctatcggc gcggggcatc
    15301 ggatatccat attgagccct atgccgatcg aaaggaaacg gctgtgcgct tccgcgtcga
    15361 cggcacctgc tttacgtaca tgaagattcc ggcggcctat cgccgggcga tcgtctcccg
    15421 cctgaagatt atggccagtc tggacattgc cgagcggcgc aagccgcagg atggcaaaat
    15481 ccggttcaag ttgggtacgg ggcaggagat cgaattgagg gtggcaacac ttcccacttc
    15541 gggcttcaat gaagatgtcg tgattcgcct gctgaccgcc cacggagctc gccgcttgga
    15601 agatctggag ttcagcgacg gaacacgccg gctcgtaggg cagttggctc aaaagccgca
    15661 tggcatcgtc ctgtgcgccg gtccgaccgg atcaggaaag accaccacct tacatgccat
    15721 tctggcgtcg attaataccg atgagcgaaa gatctggacg gcggaggatc cgatcgagat
    15781 tacgcaagat ggattgcggc aggtgcaggt tcatcccaag atcggactca cttttgccac
    15841 gaccatgagg gccttcctga gagcagatcc ggacgtgatc atgatcgggg aaatgcggga
    15901 caaggagacg gcggatattg ccattgaagc ctctctgacc ggacacttgg tgttcagtac
    15961 cattcatacg aatagcgccg tcgagactgt ggtgcgattg ctcgatctgg ggtgcgaccc
    16021 gttcaatttt tcagatgcca tgttgggagt gttggcgatg cggttgtgca agcgcatctg
    16081 tcccaactgc cgcgaggcct accatcccac gcgcagcgaa tatgacgaac tcgtgcaggc
    16141 ctttggggag ggtgattggg aaggcgtgca tcctgcatat aatccggggc tcacactgtt
    16201 tcgtggacga gggtgtgaca cgtgtaacca gaccgggtac cgtggacgag tgcccatcca
    16261 tgagttgatg gtggtgtctg attccatgaa ggcactgatt caggccaggg cgcgcaccgg
    16321 ggaactactc acactcgcaa agagcgacgg gatgaagacg ttggtgcaag acggtatcga
    16381 gaaagtgctg caaggactca ccacgtataa acaggtgcgg gcagttgcga tcaaatagtg
    16441 ccctcccgtt cgccgtgtct cacgttttgc ccagggcgat actctctgtt caaggccgct
    16501 cctgcttgtt aattatgcgc gggccaaagt cagaaacagc ccgcttgatc tggcgcctat
    16561 cctgggtgca tttttccggt ctctgcccgc agcaagaggt tggtcgcggc ttctcttgcg
    16621 cccttcccct ctcatcattc ctggctcacc agtaggtgaa gtacgccaga aatccgacaa
    16681 aaattgtcat cgaagtgatc acggaacgct cggaattgta cgccgtccag tatgaatggg
    16741 cttgatatat caatctgtta ggccatcatc ccatgtcata tccatcggca tggcacttgc
    16801 tcatctggct gttgccatgt gtgtctctat ctctctccaa cggtctgctg ctgtccaccg
    16861 ccagaggggc ttcaccctta tcgaggtgat gatagcggtc gcgattgtcg ggattctggc
    16921 catggtggcc gtccccaact atcttcaatg gaatgcccgc tatcagctga aacaagccac
    16981 aacggaattg gctggcagtc tcaacctggc ccggatggca gcgatgaatc ggaatcttgc
    17041 cgtcacagcc acattggtcc ttgtgtcggg cagggtgaat gtcgatttcg gaggtgcgtt
    17101 agcccctatt gtgttgcccc aggccgtcgt cggattcacg ggaggtccga cgattcaatt
    17161 cacccagcaa gggttgagcg gggctgcggc gaatgttcct gtggcgctga cgtcccaaca
    17221 ggggacgacc tattccgtgg tcgtcactcc ggcaggcaag gtgaactggt gtgcgcatgc
    17281 gacgtgccca tagaggccca atgaaacacc gtcaaaactc acacccctgc ttcagtctgc
    17341 tcgccgccgc acagcggggg tttacgttgt tggaagccat gattgcggcc ggtgtgctct
    17401 cggtcggact gttgggattg gcgggtctct cggggatgtc gctgggcaag aatgtcgatg
    17461 ccaacgacat gtcgcgcgtg accaatatcg ccgccgatat ggcggagcgc attcaaaata
    17521 accggcagcg tgtcttggac taccataaca ccaacacgag tgtggcctgc gcgcagagcg
    17581 cgaacactca acgcatggcg ttgggagatt gcacccaatg gcaggcgctc gtggcgaatt
    17641 ccggtctcac gggtgccacg ggacttgtca ccgcggcccg ccttgatccg gaccccaccg
    17701 cgaatccggt gacgatgaac cggttccttg tcacggtgac cgtcagctgg caaaccagac
    17761 agacggatgt gagcacggcc cggaccaaga ccgcgatatt tacgacggtg gtcgcgccgg
    17821 aataggcgta gcctggagca tgacgatgcg tagattcgac tggaaccagc agggcttcac
    17881 tctcgtggaa ctgatggcag ctgtcctgat cacggtggtg attgtggcgg cgacgatgac
    17941 cacggtggtc acttcgaatc gcgccaatgt agtcaacacg caggtcgcag acacgcagca
    18001 gaatgtgcga ttggccatcg acttgctcag ccgggatatc aaactggccg gctttaatta
    18061 caatgcgacc gatccggcaa ccggatctgt gggcgcgtgc aatgccacca tcggcgccgt
    18121 cgtcaagccg gttggactgc tgccccaaga ccaaacgccg acaggtgcgg ataccggggc
    18181 tgatagcgtg tccatggtgt tgccggtgat gaatacgaca gggtggatac tgacggcggc
    18241 ggtcggtgga acgccgaaca ataagacgta tgacaacagc atcagtcttt ccggagccgc
    18301 tatgacggag atggttgcac aaggtctcgt ggtcggatcg acgatttcca tcggcggtgc
    18361 attgtcaaag acggtgcagg ctgtcaacgc cacttcgatt ggcttcggta ccggaaactt
    18421 cgtcgatgga caattcccgg tcggcacgcc tgtgtatttg atgcaatgcg tgcggtatca
    18481 agtcgtggtg aatacgccgg cgacctgcgg cagtgacacg ccttgtctgg tgcgaaacaa
    18541 cgtgccgctg gtcgatggtg tggaagactt gcaactcgcc tacgcctgtg acgggtgcaa
    18601 tcaggccgcg cccaatccct tgtttccgga cggggttgtc gatgatcaag atgcctcctc
    18661 gacgtcggga tttcccacct tcacgcaggg agattttgtc tccaacagtg cgtgggcgat
    18721 tgccccgtgg acgccggata agatcaggct ggcgcaagtg agcctggtgg tcaggcctac
    18781 gaacgcagat gacggtcttg atgagaaggg aaccaaggcc gtcaatacca ccgggcctgt
    18841 gatcatcggc gaccataatc catctgcaga tacgggttat aacgcgagca cttatatgca
    18901 acagcgacgg cgcgtgatga tccgtacgat tcagccgaga aacctgtaac gagagacgct
    18961 gcaatgaatc tgcgacaggc cacaaccggt cgaggccaac aggataagtg cacagggctg
    19021 aagaatcagc ggggcatcgc cttgctcacc gtgatgttga tgttgttgat tctcaccata
    19081 ctcggcatcg cctccatcac ggtcaccagc atggagaatc gaatggcggg atttttccgc
    19141 acgaccgagg ctgtggtagc ggctgccgat tcgtgtgaag gactggccgc caacatcatt
    19201 cagcagacgt tgtcccctcc aggggtactc ccggcttcgt tcatcgcgcc gacggggccg
    19261 gttccgacgg ccaatgccac cacattgtac gcagaaatat atggctacga tctgccgtca
    19321 ccggctccgg caaatacacc ggccgtcaac tatgtggatg tggcgagcac agcgccgaat
    19381 tttgtgatga cgaatcttcc cgggttcacg gtcaacggcg acatcgatct cctgtatacg
    19441 catacgaaga gtgggcaagg cacaggggtg gccggcacgg aacatgtcta tcggattacc
    19501 tgcatggcga gtaaccctgc gaccggttcc agtagcacgg tgacttcggt gtacggctgt
    19561 ttggatggcg atacctgtgt caagaaaatc aactgagaga aggagcatgg cgatgaacaa
    19621 cacagtatgt attgtgatgc tcggcctgct gacactcggg cagacgatgc cttccgtgcc
    19681 gtccagcagc agcgcagaga cagagcaagc accggaggcg atgcggtttt cgacggccgg
    19741 gtttcaggcc ggcgtgattg acgaggtgca gggatcaagc attcgcatcg atggtcgtag
    19801 ctacgtgttg aagactgcgg tggttgttgt gacgcatgaa ggggagccac ttgagcttga
    19861 gcggatcatt cccacctcgc tcgtcaagtt tcatctgaaa gaaggccaca tcgacaagat
    19921 ggtggtcacg ttgccgcagt aacgtatgaa aggtcttgtc tcaactcgta gccgagaatc
    19981 gacggctgtt gtcctgcttg agataaggag gaagaacgca tgaatcgccg aacccttgtc
    20041 gctgtgatgc tggtggccgg tagcctgagt gctgcgttgt ttggttcccg tgatgctgtc
    20101 gccgtcgtgt ccaacgccga ttacacagct gtgccggctt tcgtgtcgaa taccacgatg
    20161 cccaatattc tgatcgttat ggataactcc ggtagcatga gcaatcgggc ttgtgaatcc
    20221 tcaagctgcg gagttctgcc ggatgggtcg acctcgaccg tcaccacgtt caatgccacc
    20281 acccgttaca atggtttcgc tgaccccctc cgttgttatg tttgggatgc taccgataac
    20341 cggtttgaaa atggggcggt caaggtaacg ttgaatacct cttgtactaa tgagtgggac
    20401 ggaaatttta tcaactgggc gacctttcga cgtttcgacg cggtaaaaaa agcgatgacc
    20461 ggaggaaact gtcatcatcc gaccgcttca ccggtccgca acccagatgg tacctgcaag
    20521 ccctatggct cacctgcact gccgactgtc aaggcgcaga acttggggac cagcgacgag
    20581 acgactccag cgattcccta tgccgggggc ataggtgacc tgacatatgt cggacggatc
    20641 ccgaccacgg tgcatacggg agatccagcg aacttgtatg tgcggtacga ggccgatgga
    20701 gatatgtgca tcgataatga caacacgggt gatggatgtc ccgacagtgg agggtttgcc
    20761 gaaacggagt tgaacgagat tgcgttcgcc atgtacagcg agcccaccgg tgtcatacag
    20821 caaattggcg cgaaggcacg attcgggttg gccgtattta atggctcatc aacgaacgac
    20881 ggcttacggg tgttgactcc catcgcctcg agacaatcga tcaacttcag caactccaca
    20941 gttgaaacgt tcaacaccaa tacagcagcc atgatcgact ccgtcgacga gtccactccg
    21001 gccacgtgga ctcctcttgc cgagacgctg tacgacgcga ttcgctacgt cgcgcagatc
    21061 aattctgtct actatccaac ggagtatgtc tacccgattg ctttttccag ttcaagcgca
    21121 gtcggctttg gaagcagcgg gatcggctcg atcggaacgt cggagattag cgctctgaca
    21181 ggcagcgaag cctgtccagc cgggtacata tcggggggaa ctacggcaac ctgtgcggca
    21241 ggccgcgatc cgttcttctt tggcagcaac catacgcccg cctgggcctc gacctcgcaa
    21301 gtggtgagtt gctgcaaaac cttcgtgatt gtctttacgg atggagaacc cacgcaggac
    21361 cggaatattc cagctgcatt gcgagataat cctcacaacg gtgtcgattg taccggtacc
    21421 gacaatctcg ccccgccgcg acctgtcgat gggacatgca ataaccatcc aagcacccct
    21481 gtcaccgatc tgctcggaga acacaagacc acctatgccg acaacggtag tggttatctc
    21541 gattccgtcg cctattgggc ccatacgacc gacttgcgtc cctgtaacgg agtggctgat
    21601 ggtacgatcg cggtcattat ggagaccggc cactgccttc cgggaaccca gaacgtgtcg
    21661 gtctatactt tttttgcttt cggcaatatc gccggtcgag gcatattggc tcagactgcc
    21721 aggctaggag cgttcgatga cagcaacaac gacggaattc cgcagaccaa tgagtgggat
    21781 aaggagaaca actataccgg atcttccaca ccggacggca ttccagatgc ctacttcgaa
    21841 tccgccaatg tggacgacct tcaggataag ctgctcgcga cgattgcaag cattctcagg
    21901 cggagcgcct ctggctcgtc agtctccgtg ctcgcgacgg cttccactgg cgaaggcgcg
    21961 ttatatcagt catattttta ccctagcctc gatgagactg ctacgcataa cgatgtgaaa
    22021 tggaccggtt atacgcaggc tctctttatc gatacgttcg gcaatacccg cgaagacacg
    22081 aatcaggacg gtcggctcga ttacaaggtc gacaaaatca tcaaaacccg ttttgacgcg
    22141 ttcttgaatt tggtgaaagt cgacaaatac gtggattccg atggagatgg actgccgaac
    22201 gatcaaaata ctgacggtca agtgacagtg gcggattgca atccctgcgg acaggcgttg
    22261 agtgacattg tccccatttg ggaagcggga aagcagttgg cattgaagga tgcgtccacg
    22321 cgcaacattc tcacctgggt cgattccgac catgacggcg tggtcgattc cgctgaacag
    22381 atcgcgttca cgacggcgaa tgaaccgacg ctgcgcccat atcttcgtgc cgcatcgagc
    22441 gcggaaggaa cgaagatcat caacttcgtt cgaggatgcg atgccaccac atgcgcagag
    22501 caagcgacaa ccagagatcg ccgtctcagt gtcacgaacg gagggagcac ttccttaaag
    22561 gtatggaagt tgggagatgt gattcactcc accccgacgg tggtggcggg gccgaaggat
    22621 cggcacgatg ccatctatgg cgatcagagt tattcggcct tcctgcagaa gtggtacaac
    22681 cgcaggcagg tcgcctacgt gggcggaaac gacggcatgc tgcatgcgtt caacgcgggg
    22741 tattatcatc ccggcgatga ttcgagtacc tcctataacc ctaccaccgg cggcattgag
    22801 cacggttggt ttacgaaggc tcctgcggac aattccacgg gtgctccgtt gggagatgag
    22861 ttgtgggggt tcgtacccta tgaactcctt ccgcagttgg agtttttgag ccgcgccgac
    22921 taccagcata cgtattatgt tgatttgccc ctcaaggtag cggatgtccg cattttcact
    22981 ccggacaccg atcatccaaa cgggtggggc acgatcctga tcggcggatt cagaatgggt
    23041 ggaagctgcg gtgcctgttc ggcgggaacc ggtgcgccgc cgatgacggt caatatcagc
    23101 ggcacggact acacgttcta tagctcgtat tttgcgatgg atatcaccaa tcccgatgcc
    23161 cccaaactgc tctggtcatt cagcagttcg aatttggggc tgacgaccag tgtgccggct
    23221 gtggtccgcg tgaatccagc agctgatccc aaggccagca acacaaacgc gaagtggtac
    23281 atggtggtcg ggtctggccc gaccggatat gacgccagcg tagttcagag tgcaaaagtc
    23341 tttgccgtcg accttgcgac gggcccgggt agcggcaaca gcctcgtgac gtcgtattcg
    23401 gtggggtcct ggaattcgta tatcggagac atcacggctt tcgatcgaga tctcgattac
    23461 cgtcacgatg tcgtgtattt cgggcgagtg attcatgacg gagcgcttcc ctggcggggc
    23521 aagatttatc gtttgaccat gggagcaggg ggagctacgg ccaagttcgg tacggtcacc
    23581 agtccgactc agtggggcat cgcctcggga agtgctcgcg tgcccacgga gatgctggat
    23641 acgttcgtat caggggcgga gatgggcccc gttgcgaccg cgccttccgt gaccaccgac
    23701 gatgcggcga acgtgtgggt ctttgcgggc agcgggcggt attacagcaa atccgacaag
    23761 accgatgtgt ccacgcagta cttcgtgggg ttgaaagact cggtcctcaa ttccggatgc
    23821 aatcaatcag ccggcgcgct caactgcatg gacaataatc ttgtggatgt ctcgaatgcc
    23881 accgtctgtg tggtgggagt cggcgattgt ggtcaggcca gtggaaccaa ccaagtctcc
    23941 ggcgtgtccg gtgccacgac gtttaccggc ttgatcggat tggttcagag taaagatggt
    24001 tgggtgacta aactggtcga acccgccaac ccaccgacga acccagtccc ttacggcatc
    24061 ggcgaacgtg ctgtgagtag cccaacggta ttcggagggg tggtgttttt ccccaccttt
    24121 attccaacga acgacatctg cgtgtcatcc ggaacgagcc gactctgggc actcttctat
    24181 aaaacgggga gtgcatatca agagccgatc atcggaacga ctacctcggg agcgaatcaa
    24241 acggtcaata agttcggctc tcttggagaa ggattggcgt tcggtatcgt ggtccatatg
    24301 ggctcaggac gggacggcgg cagtccgttc ggcctcctga tcaatatgag tcagggaaac
    24361 ttcggtgact gtgcaacctg cgggttcggt ggagggtctc ccggaggtct tccgagctca
    24421 aacatcgggg ccaatgttgc gattgatcca cggagtcggt acttctcatg gacgaacatg
    24481 tgatgatcgc cttggggcag gccatacacg gg
//
LOCUS       DAORQN010000012        23387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_152_length_23387_cov_176.269373, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000012 DAORQN010000000
VERSION     DAORQN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23387
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_23387_cov_176.269373"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..283
                     /gene="rpmG"
                     /locus_tag="PK614_01865"
     CDS             134..283
                     /gene="rpmG"
                     /locus_tag="PK614_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRB80990.1"
                     /translation="MRDIIDLACTVCKQRNYTTRKNKKNDPDRLERNKFCKFCRKHIA
                     HKEVK"
     gene            342..416
                     /locus_tag="PK614_01870"
     tRNA            342..416
                     /locus_tag="PK614_01870"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:375..377,aa:Trp,seq:cca)
     gene            506..700
                     /gene="secE"
                     /locus_tag="PK614_01875"
     CDS             506..700
                     /gene="secE"
                     /locus_tag="PK614_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRB80991.1"
                     /translation="MFQRLIASIREFIDGVRGELKKVSFPTRAETVGATTVVIVFCIL
                     MSLYLSMMDSVLGWLMRKVI"
     gene            774..1313
                     /gene="nusG"
                     /locus_tag="PK614_01880"
     CDS             774..1313
                     /gene="nusG"
                     /locus_tag="PK614_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRB80992.1"
                     /translation="MSNKNWYVIHTYAGFEGRVKTSLLERASQMGLTERLGQVLVPTE
                     DVIEIKDGKRRTSRRKFFPGYVLIELEAPLADETLQMIKETPKVTGFVGGGAQPTPLS
                     SEEVDSLLKQVDAGAAGPREQVRFIKGDNVRIVDGPFLGFNGAVDDVDADHSRVKVFV
                     SIFGRSTPVELGFLQVERI"
     gene            1392..1814
                     /gene="rplK"
                     /locus_tag="PK614_01885"
     CDS             1392..1814
                     /gene="rplK"
                     /locus_tag="PK614_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRB80993.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKDGDSIIPVIITVYKDRSFTFIMKTPPASDLLKKAAGIIKGSGVPHKDKVGKVSQ
                     AQVREIAQKKLSDLNAADLEGAIKIIQGTARSMGITVQ"
     gene            1859..2551
                     /gene="rplA"
                     /locus_tag="PK614_01890"
     CDS             1859..2551
                     /gene="rplA"
                     /locus_tag="PK614_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRB80994.1"
                     /translation="MGKKLTAAQEKIEPRFYGLKEAVEAVKQAAFAKYDESVDLAIRL
                     GIDPKRSDQMVRGTTALPHGTGKKLRVLVFAKGEKEQEARQAGADHVGSDDLMEKIKG
                     GWMEFDCAISTPDLMASVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVSEIRKGRVEFK
                     VEKAGIVQVPVGKVSFDVQKLYDNAQAVLESVVKAKPSSCKGRYIKSVTMSSTMGPGV
                     KLDPVALSKLWS"
     gene            2583..3089
                     /gene="rplJ"
                     /locus_tag="PK614_01895"
                     /pseudo
     CDS             2583..3089
                     /gene="rplJ"
                     /locus_tag="PK614_01895"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            3117..3518
                     /gene="rplL"
                     /locus_tag="PK614_01900"
     CDS             3117..3518
                     /gene="rplL"
                     /locus_tag="PK614_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRB80995.1"
                     /translation="MSAAAGTKLSQDELIKAIEGMSVLELADLVKGLETRFGVTAAAP
                     VAVAAVGGGGAAAAPAEEKTSFDVILVSAPADKKIQVIKVVRELTSLGLKEAKDLVEG
                     APKPVKAGATKEESDTMKKKLEESGAKVEVK"
     gene            3600..7559
                     /gene="rpoB"
                     /locus_tag="PK614_01905"
     CDS             3600..7559
                     /gene="rpoB"
                     /locus_tag="PK614_01905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRB80996.1"
                     /translation="MSESTLSEFVERKDFSRIRTSIDIPDLIEIQKRSYEEFLQMEVE
                     PDRRKDQGLQAALASVFPITDYNNTAALEFSNYSLGTPKYDERECLEQGMTFAVPLKL
                     RVRLIVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFLINGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGYSTDDLLRMYYPVEEIRVSKGKLYRKLDPEIHHGLKCSTEVMEKGGKDPLVREGAK
                     LTKVLIAKLKAAGIKEIPVTPAELVGRAVLTELVDSGKKQSLAEKNQRLTEEILEKIL
                     DSDIEEFKVIYLDTTNATLVILDTLDMERTGSKEEAMVEIYRRLRPGETPSVETARAL
                     FDNLFLSPKRYDLSPVGRLKLNKKLGLDFALEQRTLTAQDIVEVVRYLVNLKIGRGEI
                     DDIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVA
                     AVKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRI
                     CPIETPEGPNIGLITSLATYARINEFGFIEAPYRKVVKGRVSDELEYLSAIEGDKYII
                     AQANSKVDTSGRLVSETVSARSAGDFITATPDKIEYMDVSPKQVVSVATALVPFLEHD
                     DANRALMGSNMQRQAVPLLKAESPLVGTGMEAVVARDSGYVVQAKREGVVESVDATRI
                     VVRADAKEGRKRNDSGLDVYEMIKFQRSNQNTCITQTPVVRIGEPVKRGQVLADGPAI
                     DHGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGK
                     EDITRDIPNVGEEALRDLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAI
                     FGEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDHMKLQRDHQEE
                     LRIIEDEKTKKVRKLLLGKVVGRDLMDPETGDVILKKKGKLTAEILKRLPDDMVRHII
                     LSDPDEQKELEDVERRAKEQIEILQTLYDEKVGRLRRGDELPPGVIKLVKVYIAMKRK
                     IQVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHL
                     GWAARALGIKVASPVFDGASEKEIKELLKKAKLSPSGQTMLMDGRTGESFSSPVTVGY
                     MYVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASIL
                     QEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKSKD"
     gene            7701..11834
                     /gene="rpoC"
                     /locus_tag="PK614_01910"
     CDS             7701..11834
                     /gene="rpoC"
                     /locus_tag="PK614_01910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRB80997.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVMVDPGSTSMSEQELVSEEQLRSLQSEYGSGAFKV
                     GIGAEAIRELLRKVDINTQWDELHVKAKASASAALKKKYAKRLKVLEAFRRSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVGAKGEGKL
                     FGSPEEARIAYDAGALDEHARIKVRVNGTMVQTTAGRVILAEILPPMMPFADANKLMT
                     KKEMSKLIDAVYRQAGHRETVTFLDKIKDLGFHYATRAGMSICIDNMHIPSRKEDLIG
                     KAQHEVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPAKIESF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIVTEEDCGTTDGILVSALLEGGEIIQPL
                     EERLLGRLAGEDIRDPVTGEIIVSFNEEIDEEQAKAVVEAGVDRVKIRSVLTCQSARG
                     VCRLCYGRDLSRGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGQLKYMSLDAKKNADVHNAGIAVRNKEGEWVVMNRNAKIAIVDDSGREREKYPV
                     VYGAKIKVKDGDRVDVGQKLVEWDPYSLTILTETGGKVAYGDILEGVTMKEEFDEVTG
                     LSRKVIIEQSGATLRPRVSIKDDSGKTAKVSGSGGAPVARYLLPVGAHIFVEKGAMVH
                     PGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGFVKGMRK
                     VLVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPKELQKYL
                     VDEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTSFLPGSQVSKGLFDVENQR
                     VLEKDGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKE
                     NVIVGRLIPAGSGFEEYRETFVASAKAPGELSGGQPQPVAVGQHAVGSAGADPENNG"
     gene            11944..12315
                     /gene="rpsL"
                     /locus_tag="PK614_01915"
     CDS             11944..12315
                     /gene="rpsL"
                     /locus_tag="PK614_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRB80998.1"
                     /translation="MPTINQLVRKGRTLVKSKTKSPALKRCPQKRGVCLRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGSL
                     DAVGVADRKQARSKYGAKRPK"
     gene            12377..12850
                     /gene="rpsG"
                     /locus_tag="PK614_01920"
     CDS             12377..12850
                     /gene="rpsG"
                     /locus_tag="PK614_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRB80999.1"
                     /translation="MPRGQFFGHREAQPDSKYRDKLVGKFLNVLMGGGKKSTAERICY
                     GAFDLIQEKTNGGDPMKIFKSAIDNVKPVVEVKSRRVGGASYQVPVEIRPSRRVSLAL
                     RWITEYSRSRGGKSMQDRLAAELLDASNNTGASVKKREDVHRMAEANKAFAHYRW"
     gene            12963..15044
                     /gene="fusA"
                     /locus_tag="PK614_01925"
     CDS             12963..15044
                     /gene="fusA"
                     /locus_tag="PK614_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRB81000.1"
                     /translation="MARQTPLERTRNIGIMAHIDAGKTTTTERILYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWREHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVQGVEPQSETVWRQADKYEVPRIAFMNKMDRIGADFYASVQSIIDRLGANPV
                     PIQIPIGREAEFRGSVDLISMKGYFYDDETLGAKYKIGEIPADMVDQANEYRQKMLDA
                     VAEFDDQVMEKYINGQPLSEEEVRRVVRAGTIAMKVVPVLCGSAFKNKGVQQLLDGVV
                     DFLPSPVDIPPVIGVDPNTSKEVERRPSDSEPFSALAFKIMTDPFAGQLTFFRVYSGT
                     LKTGTPVLNVTKGTKDRVGRLLKMHANKREEIDIVYAGDIAAAVGLKNATTGDTLADE
                     KQPVLLEIMKFPEPVIAMAIEPKTKPDQEKMGFALQKLAQEDPSFRVRTDEETAQTII
                     AGMGELHLEIIVDRLMREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQYGH
                     VVMTVEPSESGKGLEFINKTVGGSIPKEYIPAIEKGVKERMETGVVAGFPLRDVKVTV
                     IDGSYHDVDSNEMAFKIAASMGFADACKKADPVLLEPIMKVEVLVPQDFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEATVPLMEMFGYATDLRSRTQGRATYSMEFDRYDQVPRQI
                     AEAITAKHRGE"
     gene            15080..16285
                     /gene="tuf"
                     /locus_tag="PK614_01930"
     CDS             15080..16285
                     /gene="tuf"
                     /locus_tag="PK614_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRB81001.1"
                     /translation="MAKAKFERRKPHVNIGTIGHVDHGKTTLTSALTKICADRGMAKF
                     ISYDEVAKASESQGRRDASKIMTIAISHVEYETDNRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADKVDDKELLELVELEVR
                     ELLTKYDFPGDKIPIVQGSALKAVEGDQGPLGVPSILKLLEAVDTYIPTPTRAIDKPF
                     LMPIEDVFTISGRGTVVTGRCEKGIVKVGDEIEIVGLRPTQTTIVTGVEMFRKVLDEG
                     QAGDNIGVLLRGTKKEDVERGMVLAKPKSITPHTKFKAEIYVLTKEEGGRHTPFFNGY
                     RPQFYFRTTDVTGIVTLTPGVEMVMPGDNVTVTGELISPIAMDQGLRFAVREGGKTVG
                     SGVVTEILA"
     gene            16326..16637
                     /gene="rpsJ"
                     /locus_tag="PK614_01935"
     CDS             16326..16637
                     /gene="rpsJ"
                     /locus_tag="PK614_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRB81002.1"
                     /translation="MKVDQRIRIRLRGFDYRVLDQSVVEIVETVRRSGARVVGPIPLP
                     TRIEKFTVQRSTHVDKKSREQFEIRTHKRLLDIMEPTPETMDSLMKLNLAAGVDVEIK
                     L"
     gene            16680..17300
                     /gene="rplC"
                     /locus_tag="PK614_01940"
     CDS             16680..17300
                     /gene="rplC"
                     /locus_tag="PK614_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRB81003.1"
                     /translation="MTNGLLGKKLGMTQVYDESVLAPVTVIEAGPCRVVSVKTKERDG
                     YEAVQLSFGEVKEQRLSQGELGHLKKHQAPPSRWLREFPKVGDVTVGQTIKVDMFKKG
                     DWVDVVGVSKGKGFQGVVRRHNYAGGPETHGSMFHRAPGSIGASSYPSRVWKNKALPG
                     HMGDERVTVQRLKVVDARPDENLLFVRGAVPGGVNGLLIVRKSKKS"
     gene            17304..17927
                     /gene="rplD"
                     /locus_tag="PK614_01945"
     CDS             17304..17927
                     /gene="rplD"
                     /locus_tag="PK614_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRB81004.1"
                     /translation="MPIVDVVDSKKKKVGTVDLPSEVFGCKPHVPLVHEAVVMQRACD
                     RQGTASTLRRGEVSGSGKKPWKQKHTGRARAGSLRSPVWRHGGTVFGPKPRSYAFGMP
                     RKKYRAALQSALSAKVLEGNVLVVSDLAINQAKTKLLANALSQLGISGTALLVVGDAN
                     SHLVQAGKNLSNVTVLRPEELNVYDVLRCHSLVIPQSEVNRVKEVWS"
     gene            17924..18217
                     /locus_tag="PK614_01950"
     CDS             17924..18217
                     /locus_tag="PK614_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRB81005.1"
                     /translation="MKGDLHQVLIQPLLTEKITALREQGNTVGFLVHPDANKIQIRQA
                     VESLLKVKVSRVNVVNIRGKVKRLGRFVGKRSDWKKAFVTLKEGEKLELFESV"
     gene            18251..19078
                     /gene="rplB"
                     /locus_tag="PK614_01955"
     CDS             18251..19078
                     /gene="rplB"
                     /locus_tag="PK614_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRB81006.1"
                     /translation="MALKVYKPTTPGRRGMTAIQGEKLTKKKPEKSLTGFHLRTGGRN
                     NDGRQTIRFRGGGHKRLYRQIDFRRDKAGIPAKVASIEYDPNRSSRIALLHYADGEKR
                     YILAPVGLSVNDEVQSGVGAEVRPGNALPLVNIPLGTTIHNIELKPGKGGQLIRSAGG
                     SAQVMGRDGAYVQIRLKSGEMRKVLSTCMATVGQVGNVDHENVVVGKAGRTRWKGKRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGTPAKGYKTRKNKATDKFIISRRKK"
     gene            19090..19377
                     /gene="rpsS"
                     /locus_tag="PK614_01960"
     CDS             19090..19377
                     /gene="rpsS"
                     /locus_tag="PK614_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRB81007.1"
                     /translation="MPRSITKGPFVDDHLMKKVEQMNQTKDRKLIKTWSRRSTVVPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            19422..19775
                     /gene="rplV"
                     /locus_tag="PK614_01965"
     CDS             19422..19775
                     /gene="rplV"
                     /locus_tag="PK614_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRB81008.1"
                     /translation="MAEAKAILRFVRVTPRKARIVIDMIRGQQVPMALAMLRHTPKHA
                     ARVIEKLLRSAVANAEQKELGDSDEMWVSQAVVNCGPIYKRFRARSMGRANSIQKRTS
                     HITIAVAAPGAAVNS"
     gene            19808..20476
                     /gene="rpsC"
                     /locus_tag="PK614_01970"
     CDS             19808..20476
                     /gene="rpsC"
                     /locus_tag="PK614_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRB81009.1"
                     /translation="MGQKTHPIGYRIGYNYTWSSRWYADKDYARLLHQDIKIRKMVKA
                     KLYHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAALEKEYSGQAYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMVAGRLGGAE
                     IARTEWYREGRVPLHTLRAEVDYGFAEAHTTMGQIGVKTWIYKGELLPALQLKPDSAL
                     GRLG"
     gene            20505..20921
                     /gene="rplP"
                     /locus_tag="PK614_01975"
     CDS             20505..20921
                     /gene="rplP"
                     /locus_tag="PK614_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRB81010.1"
                     /translation="MLAPKKVKFRKMQKGRMRGKAYRGGAITLGEFGLKALEPGWVTS
                     RQIEAARIAITRFVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     MYEMGGVAPDVAKQALKLAAYKLPIATKFVIRGEFQ"
     gene            20950..21171
                     /gene="rpmC"
                     /locus_tag="PK614_01980"
     CDS             20950..21171
                     /gene="rpmC"
                     /locus_tag="PK614_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRB81011.1"
                     /translation="MDVKDLSGLSIDELAEKEKQLRHELFNFRFQLGIGRLENPMQVR
                     STKRDIARLKTVRRQLELLQTQADQPVGK"
     gene            21186..21455
                     /gene="rpsQ"
                     /locus_tag="PK614_01985"
     CDS             21186..21455
                     /gene="rpsQ"
                     /locus_tag="PK614_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRB81012.1"
                     /translation="MKEGQQHRREWYGNVVSNKMNKTVVVAVERSVIHPIYKKVLRRV
                     TKLKAHDEGNVCKVGDRVQLSETRPISKDKHWRVVRVMVKGQPEK"
     gene            21516..21884
                     /gene="rplN"
                     /locus_tag="PK614_01990"
     CDS             21516..21884
                     /gene="rplN"
                     /locus_tag="PK614_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRB81013.1"
                     /translation="MIQNYTYMDVADNSGAKQVMCFHVLGGTRRRYGSLGDIVVVAVK
                     EAIPQAGVKKGDVSRAVIVRTTKEVRRDDGSYIKFDRNACVLINAQGEPVGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            21911..22237
                     /gene="rplX"
                     /locus_tag="PK614_01995"
     CDS             21911..22237
                     /gene="rplX"
                     /locus_tag="PK614_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRB81014.1"
                     /translation="MARIKTKIRKGDTVVVVTGRERGKSGKVLSVDTIQGKVLVEKLN
                     MIKRHTKPNQKLRQGGILEREAPLAISNVMFLCPVTKKPTRLGVKRQEDGRRARLSKK
                     SKEIVE"
     gene            22258..22911
                     /gene="rplE"
                     /locus_tag="PK614_02000"
     CDS             22258..22911
                     /gene="rplE"
                     /locus_tag="PK614_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRB81015.1"
                     /translation="MAKVEKGKGGKGATPKSSAKKEATVPEISQDSGNEHQFAPRLRE
                     TYREQVVPALMKEFSYGNLMQVPRLERIVLNVGMGEAIQNVKLLESASNELGIITGQK
                     PVTTRAKKAIAGFKLRQGMPIGAKVTLRSRRMWEFLDRLISLALPRIRDFRGISPKAF
                     DGRGNYTLGLKEQLIFPEIEYDSVASIHGMDITIVTTARTNDEGKALLKHLGMPFRA"
     gene            22969..23154
                     /locus_tag="PK614_02005"
     CDS             22969..23154
                     /locus_tag="PK614_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRB81016.1"
                     /translation="MSRLALKNKAAAKSKFACRDYHRCGLCGRVRGFLRRFRMCRICF
                     RFLSLKGEIPGVRKSSW"
     gene            23200..>23387
                     /gene="rpsH"
                     /locus_tag="PK614_02010"
     CDS             23200..>23387
                     /gene="rpsH"
                     /locus_tag="PK614_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRB81017.1"
                     /translation="MITDPIADLLVRLGNAARRRQDVVKVPVSKVKRQILNILGKEGF
                     ILGYGEFQEDGHPYFSVQL"
BASE COUNT         5423 a         5460 c         7417 g         5087 t
ORIGIN      
        1 tcgagagggc ggcaagaccg tcggctcggg cgtcgtcacg gaaattttag cgtaagaatg
       61 cgcgatcggc gatcagtggt cagctcgcga gttagggctg atggctgaga gttgacggct
      121 caggggatgg caaatgcgag atattatcga tttggcctgt acggtctgta agcagcgaaa
      181 ttatacgacc cgcaagaaca agaagaacga tccggatcga ttggagcgga acaagttctg
      241 taagttctgc cggaagcaca tcgctcataa ggaagtgaag taggcttttt ctgctgggtt
      301 gggcatggcc cttggctcgg cagcgctcat gttccgggtg gaggggcatg gtgtcaacgg
      361 cagcacatcg gtctccaaaa ccgagagtct aggttcaaat cctagtgccc ctgccaagcc
      421 cttgtaagtg aatgagtgcc tagaccggtg ccggctggtt ctggtcaatc aagtgcccgt
      481 gaagttctaa gagaggattg taaccgtgtt tcagcgattg atcgcctcta ttcgagaatt
      541 catcgacggt gtacgcggtg agctcaagaa ggtctctttc ccgacgaggg cggaaacggt
      601 cggcgccacc acggtcgtga tcgtgttctg tatcctcatg tccctttatc tgtcgatgat
      661 ggattccgtg ctgggctggc tgatgcgcaa ggtcatttag ttgcgtggtg cggctggaca
      721 ggtccggcgt tgcccggacg atacgatgct ggatgttcag ctgagaggag atgatgagca
      781 ataagaactg gtacgtcatc catacctatg cggggtttga gggtcgcgtg aagaccagtc
      841 tccttgagcg cgcaagtcaa atggggctca ccgagcgact tgggcaggtc ttggttccca
      901 cggaagatgt gatcgagatc aaggacggaa agcgacgtac gtcccggaga aaatttttcc
      961 cggggtatgt cttgatcgag ttggaagcgc cgctcgctga cgaaaccttg cagatgatca
     1021 aggagactcc caaggtgact gggttcgtcg gtggaggggc ccagcccact ccgctgtctt
     1081 ccgaagaggt cgattccttg ctgaagcagg tggatgccgg tgcggcgggg ccacgcgagc
     1141 aggttcggtt tatcaagggc gacaatgttc gcattgtgga tggtccgttc ctcggcttta
     1201 atggcgcggt cgatgacgtg gatgccgatc atagccgggt aaaagttttt gtgagtattt
     1261 ttggccggtc tacgccggtc gagcttggtt tcttgcaggt cgaacgtatt tgaggtcact
     1321 ggattcgaga tggtggactg agagccgttc tcagccctcg tgctcagtcc gcagttctca
     1381 gggagtaact catggccaag gaagtatcag cgcagattaa attgcagatt ccggccggca
     1441 aggccaatcc tgctccgccc gttggtccgt cattgggtca gcacggcgtg aacattatgg
     1501 agttctgcaa gcagtttaac gccaaaacgc agaaggacgg ggatagcatc atcccggtga
     1561 tcattacggt ctataaggac cggagcttta ccttcatcat gaagacgcct ccggcatcgg
     1621 acctactgaa gaaggccgcc gggatcatta agggatcggg cgtgccgcat aaggacaagg
     1681 tggggaaagt gagtcaggcc caggtccgtg aaatcgcgca gaagaagttg tccgacctga
     1741 atgcggccga tcttgagggc gccatcaaga ttatccaggg gactgcgcgt agcatgggta
     1801 tcaccgtcca gtaagtttct gcagagtgaa cctccggcgg atgtgaagga gcagtagtat
     1861 gggaaagaaa ttgaccgcgg ctcaagaaaa gatcgagccc aggttttatg gattgaaaga
     1921 ggcagtcgag gctgtcaagc aggcggcctt tgccaagtac gatgagtccg tcgatctggc
     1981 gattcgtctg ggtattgatc ccaagcggtc ggatcagatg gtgcgcggga ccacggcgtt
     2041 gccgcatggg acgggtaaga agctccgtgt gctcgtattt gccaagggcg aaaaagagca
     2101 ggaggctcgc caggcgggcg ctgatcacgt gggctcggat gacctgatgg aaaagatcaa
     2161 gggcgggtgg atggagtttg attgcgcgat ctccacgcct gatctgatgg cctctgtcgg
     2221 taagcttgga aaggtgctcg gtccccgcgg gctgatgccc aatccgaaga ccggcacggt
     2281 cacgtttgag gtcggcaagg ccgtcagtga aattcgcaag ggccgtgtcg agttcaaggt
     2341 cgagaaagcc ggaatcgtgc aagtgcctgt cggtaaggtc tcgtttgacg ttcagaagct
     2401 gtatgacaat gcgcaggccg tgctggagtc tgtggtaaaa gcgaagccct cctcctgcaa
     2461 gggccgatac atcaagagtg tgacgatgtc gagcaccatg gggcccggcg tgaagctgga
     2521 tcctgtggcg ctgtcgaagt tgtggagttg agaacaggca ggttctcagt aaggggagag
     2581 gcatgaagaa agaagagaag gcaacagcga tcgcagagtt gacggaaaaa ttcggtcgcg
     2641 cccgcttggc gattctgacg gagtgcgccg ggatgcccgt caatcagatg accgagttgc
     2701 gcagacagtt gcgcggcgcg aaggcggagt attgcgtcat caagaatacc ctggccgtgc
     2761 gcgcctcgac cggcacgatt ctcgcggacg cgaaagacca tttcaagggg ccgaccggcc
     2821 tggtgatcgg atatgacgat ccggttcttc cggcgaaaat cttgcgcgat ttcattcagg
     2881 ctgaaaagcg ctccgaaaaa atcaaggtca cggtcggagt gctggaaggc cggctagtgc
     2941 aggctgcgga tctcaacgcc attgcgcagc tgccgaaaaa agaagtgttg attgcgatgc
     3001 tgctatcggc catgcagggg ccgattcgcg gtgtggtgta cgcgttgagc ggagtgttaa
     3061 gcaagtttgt gcgagtcatt gcagccattc aggataaaaa gaaaggggag ggcgacatgt
     3121 cagcagcagc aggaaccaag ttgtcacaag atgaattgat caaggcaatc gagggtatga
     3181 gcgtgttgga attggccgat ctggtgaagg gcctcgagac gcgtttcggc gtcacggcgg
     3241 cagctccggt ggcggtcgcg gcggtgggtg gtggcggagc ggcagccgca cctgcggaag
     3301 agaagacctc gttcgacgtg atcctggtca gtgctccggc agacaagaag atccaggtca
     3361 tcaaggtggt gcgtgagctc accagcctcg gactcaagga agcgaaggac ctggtcgaag
     3421 gcgctcccaa gcctgtcaag gccggtgcca cgaaggaaga atccgacaca atgaagaaga
     3481 agctcgaaga gagcggagcg aaagtcgaag tcaagtaact gttcggtggg cggcagtgtg
     3541 tgttgtccgt tgggatggcg cggggcgttc atctccttat gagcttggag ggctaacgaa
     3601 tgtcggaatc gactctttcg gagtttgtcg aacgtaagga tttttctcgg attcgcacga
     3661 gcatcgatat tcccgatctc atcgaaatcc agaagcggtc ctacgaagag ttcctccaga
     3721 tggaagtcga gccggaccgt cgcaaggatc agggtttgca ggcggcgtta gccagcgtct
     3781 ttcccattac cgactacaac aacacggcgg ccttggagtt ctccaattat tcgttgggga
     3841 ccccgaagta cgacgaacgg gaatgtcttg agcagggcat gacgtttgcc gttcctttga
     3901 agctgcgtgt gcgcttgatt gtcttcgata aagaggacaa ggggccgaag aagaaggtgc
     3961 tcgacgttcg ggagcaggaa gtctatgtcg gcgaacttcc gctcatgacc gagcgtggta
     4021 cgttcctcat caacgggacc gagcgagtgg tggtcagcca gttgcaccgc tcgcccggcg
     4081 cgtcgttcac gcacgataag gggcggaccc atgccagcgg caaggtattg tactctgccc
     4141 ggattattcc ctatcgcggt tcctggctcg atttcgaatt cgacgcacgg gacattcttt
     4201 acgttcgcat cgatcgccgt cggaaaatgc ccgccacgat cctgttgaag gcgttcgggt
     4261 atagcaccga cgacctgttg cggatgtatt accccgtgga ggagattcgt gtctccaagg
     4321 ggaagctcta ccggaagctc gatcctgaaa tccatcacgg cttgaagtgt tcgacggaag
     4381 tgatggagaa aggcggtaag gacccgctcg ttcgcgaagg agcgaagctg acgaaggttc
     4441 tgatcgccaa gctgaaggcg gcgggcatca aggaaattcc tgtcactccc gccgagttgg
     4501 tggggcgcgc ggtgctgacg gagttggtcg acagcggcaa gaagcagtcg ctggccgaga
     4561 aaaatcagcg tctgaccgaa gagattctgg agaagatcct tgatagcgat atcgaggaat
     4621 tcaaggtcat ttatctggat acgaccaatg ccacgctggt catcctcgat acgctcgaca
     4681 tggagcgcac cgggtcgaag gaagaggcga tggtcgagat ctatcgccgt ctcaggccgg
     4741 gtgagacccc gtctgtcgaa actgcgcgag ccctgttcga taatttgttc ttaagtccga
     4801 aacggtacga tctgtcgccg gtcggacggc tcaagctcaa taagaagctg ggccttgatt
     4861 tcgcccttga gcagcggacc cttacggcgc aggacattgt cgaggtggtg cgctatctgg
     4921 tgaatctcaa gatcggtcgc ggtgagatcg acgacatcga ccacttgggg aaccgccgtg
     4981 tgcggtctgt cggcgagctg ttggaaaacc agttccggct cggccttgtg cgtatggagc
     5041 ggagcatcaa agagcgcatg aatctcctgg atatggagac cgtgttgccg cacgacttga
     5101 tcaacgccaa gccggtcgtg gcagccgtga aggagttttt cagcagcagt cagctctccc
     5161 agttcatgga ccaaacgaat cccctcgccg agattacgca caagcgtcga ctctcggccc
     5221 ttgggccggg cggattgact cgtgagcgcg cgggcttcga agtgcgcgac gtccatcctt
     5281 ctcactacag ccgcatttgt ccgattgaga cgccggaagg tccgaacatc ggcttgatta
     5341 cgtccctggc tacctacgcg cgcatcaatg aattcggatt tatcgaggcg ccctatcgga
     5401 aggttgtgaa aggtcgggtc agtgacgagt tggagtatct ctcggcgatt gagggagaca
     5461 aatacattat cgcccaagcc aattcgaagg ttgacacctc aggtcgacta gtgtccgaga
     5521 cggtgtcggc acgttcagcc ggggacttta ttactgcgac ccctgacaag atcgaataca
     5581 tggacgtgtc tccgaagcag gtcgtcagcg tcgctacggc gttggtgccg ttcctcgagc
     5641 atgacgacgc gaaccgcgcc ttgatgggat cgaacatgca gcgccaggcg gtgcctctgt
     5701 tgaaggctga atcgccgctg gtcggcaccg gcatggaagc cgtagttgcg cgtgactcag
     5761 gctatgttgt ccaggccaag cgcgaaggcg tcgtggaaag tgtcgatgct accagaattg
     5821 tggtgcgggc tgatgccaaa gagggccgca agcgaaacga ttccggcctt gatgtgtatg
     5881 aaatgatcaa gttccaacgc tccaaccaga acacctgtat cacccagacc ccggtggttc
     5941 ggatcggtga gccggtcaag agggggcagg tgctggccga tggtcccgct atcgatcatg
     6001 gggagctcgc gctcggaaag aatgtgcttg tcgcgttcat gccgtggggc gggtataact
     6061 tcgaagacgc gattctgttg agcgaaaagc tggtgcggga agatgccttc acctccatcc
     6121 atattgagga gttcgaagtc gaagcccgcg acaccaagtt gggcaaggaa gatattaccc
     6181 gcgacattcc gaatgtcggt gaagaagcgc ttcgggatct cgatgagagc ggaattatcc
     6241 ggatcggcgc ggaagtgaag cctggcgaca tcctcgtcgg gaaggtgact ccgaagggtg
     6301 agactcaact gacgccggaa gagaaactgt tgcgcgcaat tttcggtgag aaggccgggg
     6361 acgtcaagga tacgtccctc acagtgccgc ccggcgtgga aggcatcgtc gtcgatgtga
     6421 agatcttctc ccgcaaaggc ttggataagg atgaacgctc caagagtatc gagagcgaag
     6481 atcacatgaa gttgcagcgc gaccaccagg aagagctgcg gatcattgaa gatgaaaaga
     6541 ccaagaaggt ccgcaagctg ttactcggta aggtggtcgg acgcgatctg atggatcctg
     6601 agacgggcga tgtcattctg aagaagaagg gcaagctcac tgcggagatt ttgaagcggt
     6661 tgccggacga catggtgcgg cacatcattc tgagcgatcc agacgagcag aaggagctgg
     6721 aggatgtcga gcgccgcgcc aaggagcaga ttgaaatcct gcagaccctg tacgacgaaa
     6781 aagtcggtcg attgagacga ggcgatgaac ttccccccgg tgtgatcaag ctggtcaagg
     6841 tctacatcgc gatgaaacgg aagatccagg ttggcgacaa gatggcagga cgacacggga
     6901 acaagggcgt cgtctcacgc gtgttgccgg aagaggatat gccctatttg ccggacggaa
     6961 ctccagtgga aatcgttctg aatccgttgg gcgtaccgtc ccgtatgaac gttggacaaa
     7021 ttctggagac gcacttggga tgggccgcca gagcgttggg gatcaaggtg gcgagcccgg
     7081 tgttcgacgg ggcctctgag aaagagatca aagaactgct caagaaggcg aagttgtctc
     7141 caagcggaca aacgatgttg atggacgggc gtaccggtga atccttcagc agtcccgtca
     7201 cggtcggata tatgtatgtc ttgaagctcc accacctggt ggacgataaa attcacgccc
     7261 ggtcgatcgg cccatactcc ctcgtgacgc aacagccact cggaggaaaa gcgcagttcg
     7321 gtggtcagcg tttgggggag atggaggtgt gggcacttca ggcctacggg gccgcgtcca
     7381 ttctccagga attcctgact gtgaaatccg acgacgtgcc gggccggtca cgcatgtatg
     7441 aggcaattgt gaagggcgaa ccgttcctgg agcctggatt gccggaatcg ttcaacgtgt
     7501 tagtcaaaga gctgcaaagt cttgggctcg atgtcgagct ggttaaatca aaggactgat
     7561 tctagtgctg agtcttgaga tcggaagccg gatagctcac cactcagaac tgcgctcagg
     7621 cggccgttaa ggagggatca accttggaag gtgtatacac attattcgaa aagccgcgcg
     7681 actcggtgtc gttcgactcg atgcggattc gcattgcgtc gccggaaaaa atccgatcgt
     7741 ggtcatatgg cgaagttaag aagcccgaaa cgatcaatta tcgatcgttc aagcctgaaa
     7801 aggacgggtt gttttgcgcc aagattttcg gtccgatcaa ggattgggag tgcaattgcg
     7861 gcaagtacaa gcggatgaaa caccgcggca tcgtctgcga caagtgcggt gtcgaagtca
     7921 ttcaatcgaa ggtgcgccgt gaacggatgg ggcacatcga actcgccgct ccggttgcgc
     7981 atatctggtt cctgaagggt gtgccaagcc gaatcggaac gttgctcgat atgagcctca
     8041 agcagctcga aaagattctc tatttcgaga gctacgtcat ggtcgatcca ggctctacca
     8101 gcatgagcga gcaggagttg gtctcggaag agcagctgcg gtccttgcag tctgagtacg
     8161 ggagcggtgc gttcaaggtc ggcatcggcg ctgaggctat tcgcgaactc ctcaggaaag
     8221 tcgacatcaa tacgcagtgg gatgaattgc atgtgaaggc gaaggcctct gcctccgctg
     8281 cgctcaagaa aaagtatgcc aagcggttga aggtcttgga ggcgttccgc cgttccggca
     8341 ataagccgga atggatgatt atggatgtca tcccggtgct gccgccggaa ttgcgcccgc
     8401 tcgtcccgtt ggacgggggc cgttttgcga cgtcagacct caatgacctc tatcggcgag
     8461 tgattaaccg gaataatcgc ttgaagcgtt tgatcgaatt gaaggcgcca ggtgtcatca
     8521 tccgcaatga gatgcggatg ttacaagagg cagtggacgc gctgttcgat aacggccggc
     8581 gcggtcgggc gattcgcggg ccaaacaagc gtccattgaa gtcgttgagc gacatgctca
     8641 agggcaagca agggcgtttc cggcagaatt tgctaggaaa gcgagtggac tactcgggcc
     8701 gaaccgttat cgtcgtcggg ccggagttgc gtctgcacca gtgtggcttg cctaaaaaaa
     8761 tggccctgga attgttcaag ccgtttatct tccacaagct ggaagagcgt ggcgcggcca
     8821 cgaccattaa gagtgccaag cggctggttg aaaaagagcg acctgaagtg tgggatgtgt
     8881 tggatgaagt gatccgggag cacccggtgt tactcaatcg cgctccaacc ctgcaccgat
     8941 tgggtattca ggcctttgat ccggtcctgg ttgagggcaa ggcgatccgg cttcacccgt
     9001 tggtttgcgc ggcattcaac gccgacttcg acggagacca aatggcggtc cacgtgccgt
     9061 tgtccgtgga ggcgcaggtc gaagctcgtg tgctgatgat gtcgatcaac aacattctgt
     9121 ctccggccaa cgggaaaccg attgccgttc cctcgcaaga catggtgctt ggttgttact
     9181 ggttgaccaa ggagcgggtc ggcgcgaagg gtgaaggtaa gttgttcggc tcacctgaag
     9241 aagcgcggat tgcgtacgat gccggagcgc tggacgagca tgcgcggatt aaggtccgcg
     9301 tcaacggaac gatggtgcag acgaccgccg gccgcgtcat cctggccgaa attttgcctc
     9361 cgatgatgcc gttcgcggac gccaataagc tgatgactaa gaaggaaatg tcgaagctga
     9421 tcgacgcggt ctatcggcag gcggggcatc gcgagacggt gacattcctg gataagatca
     9481 aggatctcgg gttccactat gcaacccggg cggggatgtc catctgtatc gataacatgc
     9541 atattccatc ccgtaaggaa gacctgatcg ggaaggccca gcatgaagtc aacgaaatcg
     9601 aaaaacagta ttccgaaggt ttgatcacca acggtgagcg atacaacaaa gtcatcgaca
     9661 tttgggcgca cgtcaccgag caggtggcca atgagatgat gaaggaattg ggcgccggag
     9721 gcgatccagc gaagatcgag tccttcaatc cgatcttcat gatggccgat tctggtgcga
     9781 ggggtagttc gcagcagatt cgtcagctcg gcggtatgcg cggactgatg gccaaaccat
     9841 ccggtgaaat catcgaaacc ccgatcacgg cgaacttccg tgaagggttg acggtgttgc
     9901 aatactttat ctccacacac ggtgcccgaa aaggtttggc ggacacggcg ctcaagacgg
     9961 ccaattcagg ttatctgacg cgtcgattgg tcgacatcgc ccaggacgtc atcgtgaccg
    10021 aggaggattg cggcacgacg gatggtatct tggtgagtgc cctcctcgaa ggcggagaaa
    10081 tcattcagcc cttggaagag cgcctccttg gccggcttgc cggcgaggac attcgcgatc
    10141 cggtgacggg tgagatcatc gtttccttca acgaagaaat cgacgaagag caggcgaagg
    10201 cggtggtgga agccggcgtc gatcgggtca aaatccgatc agtgctgacc tgccaatctg
    10261 ctcgtggcgt ctgccgactc tgctacggac gtgatctctc ccgcggacgt cttgttgaaa
    10321 aaggcgagcc tgttggagtc attgcggcgc aatccatcgg tgagcctggt actcagttga
    10381 cgatgcgtac attccatatc ggtggtacgg ccagtaaagt ggtcgaacag acggtgttgg
    10441 aggccaagca cgcgggtcag ttgaaataca tgagcctgga tgcgaagaaa aatgccgatg
    10501 tgcacaatgc gggcattgcg gttcggaaca aagagggcga atgggttgtc atgaaccgca
    10561 acgccaaaat cgcgatcgtt gacgacagcg gtcgagaacg cgaaaaatat ccggttgtct
    10621 atggcgccaa gatcaaggtg aaggatggcg atcgggttga cgttggtcag aaattggtcg
    10681 aatgggatcc ttattcgttg accatcctga ccgagactgg cggaaaggtt gcctatggcg
    10741 acattctcga aggcgtcacc atgaaggaag aattcgacga agtgaccgga ctgtcacgca
    10801 aggtcatcat cgagcagagc ggggccacgc ttcgaccgcg cgtctcgatc aaggatgata
    10861 gtggaaagac ggctaaagtg tccgggtctg gaggagcgcc ggttgcacgc tatctcttgc
    10921 cggtcggcgc tcacattttc gtcgagaagg gtgcgatggt gcatcccggc gacgtcctgg
    10981 ccaagattcc gcgtgaaacg accaagacca aggacatcac cggaggtctc ccgcgcgttg
    11041 ccgaactctt tgaggcacgc aagcctaagg agcaggcggt gatcagtgag atcgacggcg
    11101 aagtgtcgta tggcggcttt gtgaagggta tgcggaaggt gctggtcgac aataagatgg
    11161 gcgacgtcaa ggagtatttc attcccaagg gtaagcacgt caacgtccat gagggcgatt
    11221 gggttcgggc cggtgagccg ttgatggacg gttcagccaa tccccacgac atcctggacg
    11281 tgctcgggcc aaaggagctg cagaaatacc tggtggatga agtgcaagat gtctatcgct
    11341 tgcaaggtgt gtcgatcaac gacaagcata ttgagatcat cgtgcggcag atgctccgca
    11401 aggtcaggat tgaggaccca ggggatacct cgttcttgcc aggcagccag gtgagcaagg
    11461 ggctcttcga tgtagaaaat cagcgagtct tggagaaaga cgggaagccg gctctcggta
    11521 agccggtctt gctgggcatc accaaggcag ccctgaccac ggatagcttc atttctgcgg
    11581 cgtcgttcca agagacgact cgcgtgctga ccgaggccgc gatcaatggc cgcgaggata
    11641 atctcctcgg cttgaaggaa aacgtgatcg tgggccgcct catacctgcc ggaagcggat
    11701 ttgaggagta tcgggaaacg ttcgtggcta gtgccaaggc ccctggggag ttgtctggtg
    11761 gccaaccgca gccggttgcc gtgggtcagc atgcagtcgg ttccgccggc gctgatccag
    11821 aaaataatgg atgaaaagaa aaagaacgtt cttgacataa gtagtacaca tctctataat
    11881 ccgacggctt cgctcacgag catttttgag caactgtctg tttttcaaga aagagtatag
    11941 tgcatgccaa cgattaatca gctggtgcga aaaggtcgga cgctcgtaaa gtcaaagacg
    12001 aaaagtccgg cgctcaagcg ctgcccgcaa aagcgcggcg tctgtctccg tgtctatact
    12061 accactccga agaagcctaa ctccgcgctt cggaaagtgg ctcgcgtccg tctgacgaac
    12121 gggatggaag ttacgactta tattcctggt gtcggacaca acctccagga gcactcgatt
    12181 gtgctggttc gtggtggtcg cgtaaaggac ttgccgggtg tgcgttatca catcgttcgt
    12241 ggctccttgg atgccgtcgg tgttgccgat cggaagcagg cgcgttcgaa gtatggggcg
    12301 aagcggccca agtagtaatt gattgtgtca ttgcgtgatt gcgcagtgaa aaccgatttt
    12361 atttaatagg ataacgatgc cacgcggaca attttttggt catcgagagg cgcagccgga
    12421 ctctaagtat cgcgataagc ttgtcgggaa gtttttgaat gtcctgatgg gcggtggaaa
    12481 gaagagcact gctgagcgga tctgttatgg ggcgtttgat ttgattcagg agaaaaccaa
    12541 tggtggtgat ccgatgaaga ttttcaagtc tgccattgat aacgtgaagc cggtagttga
    12601 ggtgaagtcg cgtcgggttg gtggtgcttc ttatcaggtt cctgttgaga tacgcccgtc
    12661 tcgtcgtgtt tcgctggctc tgcgatggat tactgaatac tcccgttcgc gtggcgggaa
    12721 aagtatgcag gatcggcttg ccgctgaatt gttggatgcc tccaataata ccggtgcgtc
    12781 agtgaagaag cgtgaggatg ttcatcggat ggccgaagcc aataaggctt ttgctcatta
    12841 tcgttggtag tgttgtgtcg tgctgctgat gagccgggct gcaatgtggc cggataagat
    12901 tcggcattgc ggtggaaggt ttgcggctcg gaaggcagca cgttcgtttt tttgggggag
    12961 ttgtggctag gcagacaccg ttagagcgaa caagaaatat cggcatcatg gcgcacattg
    13021 atgccggcaa gactacgact acagagcgaa ttctctacta tacgggaatg acgcataaga
    13081 tgggcgaggt tcatgagggc gccgccacca tggattggat ggagcaggag cgtgagcgtg
    13141 gtattacgat cacggctgct gctacgacgt gtttctggcg tgaacaccgt atcaatatta
    13201 tcgatacgcc tggtcacgta gactttacga tcgaggtcga gcggtccctg cgtgtgctgg
    13261 atggtgcggt cgccgctttt gactccgttc agggtgtcga gcctcagtct gaaaccgtct
    13321 ggcgtcaagc tgataagtac gaagtcccgc gtatcgcttt catgaataag atggatcgaa
    13381 ttggcgcgga cttttatgcc agtgttcagt cgattatcga tcggctgggt gccaatcccg
    13441 tgcccattca gattccgatt gggcgggaag ctgagtttcg cgggtctgtc gatctcattt
    13501 cgatgaaggg ctatttttac gacgacgaga cgctgggcgc caagtataag atcggtgaga
    13561 ttcctgctga tatggtcgat caggccaatg agtatcgtca gaagatgctc gatgcagtgg
    13621 ctgagttcga cgatcaagtg atggagaagt atatcaacgg tcagcccttg agcgaggagg
    13681 aggtgcggcg ggtcgttcgc gccgggacta tcgccatgaa ggtggtgcct gtcttgtgcg
    13741 gatccgcatt taagaataag ggcgttcagc aacttctgga tggcgtcgtt gatttccttc
    13801 cctctccggt agatattcct cctgtgattg gcgtcgatcc caatacttcg aaggaagtcg
    13861 aaaggcgtcc ctctgattcc gaaccgtttt cggctttggc cttcaagatc atgacggatc
    13921 cgttcgctgg tcaactcaca tttttccgtg tctactcggg cacgttgaag accggtacgc
    13981 cggtcctgaa tgtgaccaaa ggaacgaagg atcgtgttgg tcgtctgttg aagatgcacg
    14041 ccaacaagcg tgaagagatc gatatcgtct atgccggtga tattgccgct gcggttggtc
    14101 ttaagaatgc tactaccggt gatacgctgg ccgatgagaa gcagcctgtg ctgctcgaaa
    14161 ttatgaagtt ccctgagccg gttattgcta tggcgattga gccaaagacc aagccggacc
    14221 aggaaaagat gggattcgcc ttgcagaagt tggcgcagga agatccttcg ttccgggtgc
    14281 ggaccgatga agagactgcc cagacgatta ttgccggtat gggtgagttg catcttgaga
    14341 tcattgttga tcggctgatg cgtgaattta aggttgaggc caatgtcggc aagcctgaag
    14401 tcgcgtttcg ggaaaccatc cgtcgaaagg ctgaggcgga gtcaaagtac atcaagcaga
    14461 ctggtggtcg tggccagtac ggtcacgtgg ttatgactgt agagccttcg gagtccggta
    14521 aggggctgga gtttattaat aagactgttg gtgggtccat tccgaaggaa tatattcccg
    14581 ccattgaaaa aggtgtcaag gagcgcatgg agaccggcgt cgtggctggg ttcccgttgc
    14641 gcgatgtcaa ggtcaccgtc attgatggct cctaccacga tgtcgactcg aatgaaatgg
    14701 cgtttaagat cgccgcctcg atgggatttg ctgacgcctg taagaaggct gatccggtct
    14761 tgcttgagcc gatcatgaag gttgaggtgc tcgtgcctca agatttcatg ggtgatgtca
    14821 ttggcaattt gaacggtcgc agagggaagg ttcagggcat gaaagtgcgc gccggtgctc
    14881 aggctatcga ggcgaccgtg ccgctcatgg aaatgttcgg ctatgcgacc gatttgcgtt
    14941 ccagaacgca ggggcgcgca acatacagca tggaattcga ccgctacgac caggtgccgc
    15001 gtcaaattgc ggaagcaatt accgcaaagc atcggggcga gtaaagtgtt tcgttttgtg
    15061 aattagggga ggattgggga tggcgaaggc gaagtttgag cgacgaaagc cccacgtgaa
    15121 catcgggacg atcgggcacg tggaccatgg caagacgacg ctgacgagtg cgctgaccaa
    15181 gatctgcgcg gatcgcggga tggcgaaatt catcagctat gacgaagtgg cgaaggcgag
    15241 cgagagtcag gggcgccggg atgccagcaa gatcatgacg atcgcgatca gccacgtcga
    15301 gtacgagacg gacaaccggc actatgcgca cgttgactgc ccgggccacg cggattatgt
    15361 gaagaacatg atcaccggcg ccgcgcagat ggacggggcg atcctggtgg tgagtgccgc
    15421 cgacggtccc atgccgcaga cgcgggagca cattttgttg gcgcgccaag tcggcgtccc
    15481 ctacatcgtg gtgtttctga acaaggcgga caaggtcgac gacaaggaat tgctggaatt
    15541 ggtcgagctc gaagtgcggg agctgctgac gaagtacgac ttcccgggtg ataagattcc
    15601 gatcgtgcag gggtcggcgc tgaaggcggt cgagggggat caggggccgt tgggcgtgcc
    15661 gtccattctg aagttgctcg aagccgtcga tacctacatt ccgacgccga cccgggccat
    15721 tgataagccg ttcctgatgc cgatcgagga cgtgtttacg atcagtgggc gcggcacggt
    15781 ggtgacgggc cggtgcgaga aggggatcgt gaaggtcggc gacgaaatcg aaatcgtcgg
    15841 cttgcggccc acgcagacga ccatcgtgac gggcgtggaa atgttccgca aggtgctcga
    15901 tgaggggcaa gcgggcgata acatcggcgt cttgttgcgc ggcacgaaga aagaggatgt
    15961 ggagcggggc atggtgttgg ccaagccgaa gagcatcacg ccgcacacga aattcaaggc
    16021 cgagatctat gtgttgacca aggaagaggg cgggcgtcat acgccgttct tcaacggcta
    16081 ccggccgcag ttctacttcc ggacgaccga cgtgacgggg atcgtgacgt taacgccggg
    16141 cgtggagatg gtgatgccgg gagacaatgt gacggtcacg ggcgagttga tcagcccgat
    16201 cgccatggat cagggattgc ggtttgcggt tcgagagggc ggcaagaccg tcggctcggg
    16261 cgtcgtcacg gaaattttag cgtagtgatg tcccggtcca ttggccggca tgtgaggagt
    16321 tgagcgtgaa agtcgatcaa agaattcgaa tcaggctgcg cggttttgat tatcgcgtgc
    16381 ttgatcaatc ggtagtcgaa atcgttgaga ctgtaaggcg tagtggtgct cgagtagtcg
    16441 ggcccattcc cttgccgact cgaattgaaa agtttacagt gcagcgctcg actcacgttg
    16501 ataagaagtc tcgtgagcaa tttgagatcc gcactcacaa gcggctgcta gatattatgg
    16561 agccgacgcc ggaaacgatg gactcgctca tgaagttgaa tttggcggcc ggtgttgacg
    16621 tggagattaa gctctgacga ttgggttcgg ccaatctcat ttgagctgac aggaagacga
    16681 tgacaaacgg attattggga aaaaagttgg ggatgaccca agtttatgac gagagtgtgt
    16741 tggctccggt cacggtgatc gaggcaggcc cttgccgcgt tgtttcagtc aagacgaaag
    16801 agcgtgacgg ctacgaggct gtgcagctgt ctttcgggga agtcaaggag cagaggttgt
    16861 cgcagggcga gctcggtcat ctgaaaaagc atcaagctcc tccgagtcgt tggttgcgcg
    16921 agtttccgaa agtcggggat gtgactgtcg gccagacgat taaggtcgat atgtttaaaa
    16981 aaggtgattg ggttgatgtg gtcggtgtct ctaaaggtaa gggattccag ggggtggttc
    17041 ggcgccacaa ctatgcgggt ggccctgaaa cgcacggttc tatgttccac cgcgcgccag
    17101 gatccatcgg cgccagttcg tatccgtccc gtgtttggaa aaataaggcc ctgcctgggc
    17161 atatggggga tgagcgcgtc acggttcagc gactaaaggt ggtcgacgcc cgtccagatg
    17221 aaaacctgtt gttcgtccgc ggagcagtcc ctggtggtgt gaatggattg cttattgtgc
    17281 ggaaatcgaa aaagagttga gccatgccga tcgttgatgt tgttgactcg aagaagaaga
    17341 aagtcggtac agttgacttg ccgagcgaag tattcggctg taagccgcat gtcccgctgg
    17401 tgcatgaagc cgttgtgatg cagcgtgctt gtgatcgcca gggaaccgct tcaactttgc
    17461 gcaggggcga ggtgagtggt tcgggtaaga agccgtggaa gcaaaagcat accggacggg
    17521 ctcgtgctgg ttctttgcgc tcacccgtct ggaggcatgg agggaccgtt ttcggtccga
    17581 agccaagaag ctacgcgttt ggcatgccaa gaaagaaata ccgtgcagcg ctgcagagcg
    17641 ccttgtcggc aaaagtgctg gaagggaatg ttcttgtagt gtctgattta gccatcaatc
    17701 aagccaagac gaagttgctc gccaacgcgc tgtcgcaact cggcatttcc ggcacggcat
    17761 tgcttgttgt tggtgacgcc aattcgcatc tcgtccaggc aggaaagaac ctttcgaatg
    17821 tcacggtctt aaggcctgaa gagctgaatg tgtacgacgt cctccgatgc cactccttgg
    17881 tcatcccgca aagtgaagta aatcgggtta aggaggtgtg gtcatgaagg gcgacctcca
    17941 tcaagtatta atacagccgt tgttgactga aaagattacg gccttgcgtg agcaaggtaa
    18001 taccgttggg tttctggtgc atcctgatgc gaataagatt cagatcaggc aggccgttga
    18061 atcgctttta aaggtcaagg tctcccgcgt gaacgtggtc aatattcgcg gcaaggtaaa
    18121 gcgactcggt cgatttgttg gaaaacgctc tgattggaag aaagcatttg tcacgttgaa
    18181 agaaggcgag aagctggagc tattcgaaag cgtttagccg gtgggcctct cgatagagtg
    18241 agaatcagct atggcattaa aagtttataa accgacgaca cctggtcggc gtggcatgac
    18301 cgctatccag ggagagaagc tgactaagaa gaagcctgag aagtcattga ccggcttcca
    18361 cctccggacg ggcgggcgta acaatgacgg acgccaaacc attcgatttc gtgggggtgg
    18421 gcataagcga ctttatcgtc agatcgattt tcggcgggat aaggctggta ttccagctaa
    18481 ggtcgcgtct attgaatacg atcccaatcg atcgtcaaga attgccttgc tgcactatgc
    18541 ggatggtgag aagcgttaca ttcttgcccc agtaggattg tccgtcaatg atgaggtgca
    18601 gtccggtgtt ggcgccgagg tccggccggg gaatgcgttg ccgctcgtga atattcccct
    18661 tggtaccacg attcacaaca ttgaattgaa gcctggcaag ggtggtcagt tgatccgcag
    18721 cgccggggga tctgcccaag ttatggggcg tgatggggcc tacgttcaga ttcgtctgaa
    18781 gtccggcgag atgcgcaagg tcctgtcgac ttgcatggct acggttggtc aggtcggtaa
    18841 cgttgatcat gaaaacgttg ttgtcggcaa ggctggtcgt acacggtgga agggtaagcg
    18901 tccccacgtt cgaggtgtgg ttatgaaccc tgtcgaccac ccgcatggtg gtggagaagg
    18961 aaagtcgggg cagggcaatc ctcaccctgt gtctccctgg ggtacgccag ctaagggcta
    19021 taagacccgc aagaacaagg cgaccgataa gtttatcatc tcgcgtagga agaagtagga
    19081 gagccgagaa tgcctcgttc aattaccaag ggtccattcg ttgacgatca tctgatgaaa
    19141 aaagttgagc agatgaatca gacgaaggat cggaagctga ttaaaacctg gtcacgtcgt
    19201 tctacggttg tgccggatat gattgggcac acgtttgccg tgcacaatgg gaagaagttt
    19261 attcccgtct tcgtcacaga aaacatggtt ggccataagc tcggtgaatt tgctcctacc
    19321 cggtttttta aagggcatgg gcaggcaaag accgaaaaag cagtggctct taagtaatcg
    19381 ctggttcgct gaaggatcgg aaggtttggg tggggtcagc tatggcagaa gcaaaagcaa
    19441 tattacgttt tgtgagagtg acgcctagga aggcgcgcat tgtcatcgac atgattcgcg
    19501 ggcagcaggt cccgatggcg ttggctatgc tccggcacac tccgaagcat gccgcacgtg
    19561 tcattgagaa gctccttcgt tccgccgtcg ccaatgcgga gcaaaaggaa ttgggggata
    19621 gtgacgaaat gtgggtgtct caagccgtgg tgaactgcgg cccaatttat aagcgttttc
    19681 gtgcgcgatc gatgggccgg gccaactcaa ttcagaagcg caccagccat atcacaattg
    19741 cggttgctgc tcctggcgcc gcggtgaata gctagagctt ttacacttag actgagggtt
    19801 attcttcatg ggtcagaaga cacatccaat tgggtatcgc atcgggtata actacacgtg
    19861 gagctcccgc tggtacgcgg ataaggacta tgccaggctt ttgcatcaag acattaagat
    19921 ccgaaagatg gtgaaagcca agttgtacca tgctggagtc gccaaagtgg aaattgagcg
    19981 ctcaggcgac cagacgaggg tgatcatcca tacggctcgt ccaggcatta tcattgggcg
    20041 caaaggcgcc gaggttgaca agctcaaggc cgctctggag aaggaatact ctggtcaggc
    20101 gtacatcacg gtcaaagaaa ttaagaagcc tgaattggat gcgcaattgg ttagcgagaa
    20161 tgtggcaacc caattggaga agcgcgttgc atttcgtcgc gcgatgaagc gcagtgtgca
    20221 gtctgctctt cgtctggggg cccaaggaat caagatcatg gtagcgggac gtttgggcgg
    20281 tgcagaaatc gctcgaaccg agtggtatcg agaagggcgt gtaccccttc ataccttgcg
    20341 tgccgaggtg gactatggtt tcgccgaggc gcataccacg atggggcaga ttggtgtaaa
    20401 gacctggatc tacaagggtg aactgttgcc tgcccttcaa ctgaaaccag actcggcact
    20461 aggccggctt ggctgaggag cctcgataag gaaaaggtgg gtttgtgtta gcgccaaaga
    20521 aagttaagtt cagaaaaatg caaaagggac ggatgcgggg gaaggcctac cgcggaggag
    20581 ctatcacgct tggtgagttt ggcttgaagg cattggagcc cggttgggtc accagccgcc
    20641 aaattgaggc agcgcgtatt gctatcactc gtttcgtcaa gcgtggtggt caggtgtgga
    20701 caaggatttt ccctgataaa ccaattacca agaagccagc agagactcgc atgggtaaag
    20761 gtaaggggaa ccctgagtat tgggtggccg tagtcaagcc aggtcggatc atgtatgaaa
    20821 tgggtggcgt ggctcccgat gtcgctaagc aggcactgaa gttggctgct tacaagcttc
    20881 ctatcgcaac caaattcgtt atccgcggtg agtttcagta acctgtagcg cgtgttgagt
    20941 ggtgatgtga tggatgtaaa agatctgagc ggtctctcaa ttgatgagtt ggccgaaaaa
    21001 gaaaagcagt tgcgtcatga actgtttaat ttccgattcc agcttgggat cgggcggttg
    21061 gagaacccga tgcaagtgcg aagtacgaag cgcgatattg ctcggctcaa aacagtcagg
    21121 cgccaactgg aattgctgca gacacaagcc gaccaaccgg ttggtaagta ggagagaagg
    21181 ggcgtatgaa ggaaggccaa caacatcgac gtgaatggta tggcaacgtc gtcagcaaca
    21241 agatgaataa gacggtggtc gttgccgtgg agcgctcggt aattcatccc atctacaaga
    21301 aggttcttcg ccgggtgaca aagcttaagg cgcacgatga aggtaacgtg tgcaaagtag
    21361 gggatcgggt acagctcagt gagacacgtc ccatcagcaa agataagcat tggcgtgtgg
    21421 tacgcgtcat ggtcaagggg caacctgaaa agtaactacg gaacacactt gaaccgctga
    21481 ggtgatttga tccttggcag tgagtagggc agggcatgat tcagaattac acatacatgg
    21541 atgtggccga taattcaggc gcgaagcaag tcatgtgttt tcatgtgctt ggtgggacca
    21601 ggcgtcggta cggatcgtta ggcgacattg ttgtcgtggc cgtcaaggaa gcgattcctc
    21661 aggccggtgt gaagaagggt gatgtgagtc gcgcagtcat cgtccgtacg acgaaggaag
    21721 tgcgccgtga cgacgggtct tacatcaagt ttgaccgcaa tgcctgtgtg ttgatcaacg
    21781 cgcaagggga gcctgttggt acccgtattt tcggcccagt cgctcgtgag cttcgctgga
    21841 agaagtttat gaaaattatc tcgcttgcgc cggaagtctt gtagttgaag gaagaaaggt
    21901 tctggtaggg atggcacgaa tcaaaacgaa aattcgcaag ggcgacaccg tcgtggtggt
    21961 gaccgggcgt gagcgtggca agtcaggcaa ggtcttgtct gtggacacta tacagggcaa
    22021 ggtcttggtt gaaaagttga acatgatcaa gcgtcacacc aagccgaatc aaaaacttcg
    22081 tcaaggtggg attcttgagc gcgaggcccc gcttgccatt tccaatgtga tgtttttgtg
    22141 tccagttacg aagaagccaa ctcggttggg ggtcaagcgc caggaagatg gtcggcgggc
    22201 ccggctgagt aagaaatcca aagaaatcgt cgaatagtcg gaacaggaaa gacgttcatg
    22261 gcaaaagtag agaagggtaa gggcggcaaa ggagctactc caaagtcgtc agcaaagaag
    22321 gaagcgacag ttcctgagat ctctcaggac tctggcaatg agcatcagtt cgcgccgcga
    22381 ctcagggaga cttatcggga acaggtggtt ccggctctca tgaaagagtt cagctacgga
    22441 aatttgatgc aggttccaag gcttgaaaga atcgtcttga acgtgggtat gggtgaggcg
    22501 attcagaacg tcaagttact tgagagtgcg tcgaatgagc tcgggatcat caccggtcaa
    22561 aagcctgtca ctacaagagc gaaaaaggcg atcgccggtt ttaagttgag gcaaggcatg
    22621 ccaatcggtg cgaaggttac gctcagaagc cgacgcatgt gggagttcct cgaccgttta
    22681 atttctctcg cgctcccacg aatccgtgac tttcgtggca tatcccccaa ggcatttgat
    22741 ggccgtggca actatacgct cggcttaaaa gagcaattga ttttcccaga gattgagtac
    22801 gatagtgtcg cctccatcca cggtatggat atcaccattg tcactaccgc gagaacgaat
    22861 gacgagggca aggcgttgct caagcacttg ggaatgccgt ttcgggccta gtgcgcattt
    22921 agttcggtag gtgtggcgtt gtaggcaagt acgagtaagg gggattcagt gtctagatta
    22981 gcgctcaaga ataaggccgc cgcgaagtca aagttcgcct gtcgtgacta tcaccgatgc
    23041 gggctctgtg gtcgcgtccg tggatttttg cgacgttttc ggatgtgccg tatttgtttt
    23101 cgatttctga gcctcaaggg tgaaattccc ggagtgcgaa agtcaagctg gtagtcggcc
    23161 tgctggaagc ttgccgctgc aagcgtagaa gaggaatgca tgattactga tccaattgct
    23221 gatctgttag tgagattagg gaacgctgct cgtcggcgtc aagatgtggt gaaggtccca
    23281 gtttccaagg taaagcggca gattctgaac atccttggca aggagggatt cattcttggt
    23341 tacggcgaat ttcaagaaga tggtcatccc tacttctccg tccagct
//
LOCUS       DAORQN010000013        22736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_161_length_22736_cov_29.557118, whole genome shotgun sequence.
ACCESSION   DAORQN010000013 DAORQN010000000
VERSION     DAORQN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161_length_22736_cov_29.557118"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3270
                     /gene="smc"
                     /locus_tag="PK614_02015"
     CDS             <1..3270
                     /gene="smc"
                     /locus_tag="PK614_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRB81018.1"
                     /translation="IETRAGSKGHTVIEQGRIEQILQASPQDRRELIEETAGIVRYKK
                     QKAEALRKLEATQQNLVRVRDIVAEVKKQLNSLERQARQARSYQTLQQEARGLEIELL
                     SRDYRTMHADLESVDHEAREVEAQEAEQVAEQARLDSEQETIRLRMNDAAEAISRARD
                     TLAGTEQRQSQALTAAEVERNRTELFERQRMQAAQEMERLAADQEQAQSEIETLRAML
                     LQLEGDIVEQEAQFQQADVEAKSLAGHRASAVAEEERARKDVLNLAVLVANTEQSLTQ
                     VTARQQETAARAERVSREQEQLVAQVAGLDQQRDLLAAQREEASGRIQELMAERQAAI
                     ERIEGLGGQITGLDRDIVKFSEDVAGVESRLGALQGVVREEMGYGREGEEEGTALKTC
                     DGVREALAEWLVIPPGLDRAVETILGERVRGWLVDEPSAACRAVEFLKGKELGRGAFL
                     PQQLRWAPEQAALDSSASWWPTLNGQAGVVGRATDLIRAEGASAATLSYLFDGIVFVE
                     SLDVALQLWQQHQWAAPAGPTYVTLSGETLDAAGVMTGGGSSASGGLLQRRREVLELE
                     ARRTAAVQALEQARSAREDAAAELGLGREDEQRLGRAIREAEMLELSLQKDDAGVERQ
                     RGELHRRMEVLAEELQRGLAEQARLQEEFQSSQAQLGQWVAEKVGQETGLLHVRERLV
                     VIESQSLAIQQRLTEVRLSLESMRGRREHATNDIARFTQRLDAAQRRATDLEEQVAGL
                     VEATENSREEQTRQEALCRELGAEVDGIKAELVAAQERQAQEMAGLHAVEETLSQVRQ
                     SLSALHDRRMRAEVRKAEVKAHLSTIESTLAGTYQIEPATLLLPASDQPVPEGEEPAA
                     PVSMLETPQLREQIQKIRERLDRMGAINLAAIDEHRELEARYQFLTTQEQDLSTSIAS
                     LKEIIQRINRTTKDMFVETFNELQQKFRDVFSQFFPGGRAELQLVEEPLEEGVEDNGA
                     REPGVEIVAQPPGKRLKSITMLSGGEKTLTAMALLIASFLIRPTPFCILDEIDAPLDE
                     ENIGRFTSVLRSLSSTAQFMVITHNKRTMAMADSLFGVTMEEPGVSTLISVKLGDLQP
                     V"
     gene            3493..5670
                     /gene="shc"
                     /locus_tag="PK614_02020"
     CDS             3493..5670
                     /gene="shc"
                     /locus_tag="PK614_02020"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HRB81019.1"
                     /translation="MRLLKNLFNRLSDSLLVSVPSRIRAARDFASAPVLRLVSNKSAV
                     PASGDSAAKRAPAHSTGQVEALEEALRKSQAWFLARQDASEGYWVAELEADTTLTSEY
                     LMLRRFLDCIDPERERKAVRYLKSAQLPDGGWSIYHGGPAEISASVKAYFALKLSGVS
                     ADEPFMVNARTCILDKGGVVAANVFTKIALALFGQYDWRGVPSMPPEIMLLPKRFYFS
                     IYAISYWSRAVLIPLLIIFAKRPLCHVPPELGIDELYTEPPAEIDYGMVPPFKKDRTW
                     FTARNFFINLDALLKIYDRSPVEWVRQKALKCAENWMLDHMKGSGGLGAIYPAMANSV
                     MALHCLGYKNDDPLMVKAIREIEELEVHDTVQDNGQCVDAMHLQPCHSPIWDTALLIN
                     ALIEAGMPQDHPALQKASTWLLSKQTKTVGDWIISAPGAEPGGWYFQFENELFPDVDD
                     SAVVLMALAKVHLSDEAQQRLAIRRGCRWVTSMQGSDGGWGAYDVDNNRIVFNYIPFA
                     DHRALLDPSTADLAGRCLEMLATLGYDWTHPAVASALTFVKNDQEQDGSWYGRWGVNY
                     IYGTWSVLSGLRAIGEDLSSPYIRRAVSWVESKQNPDGGWGESCLSYGDVSQSGRGDS
                     TPSQTAWALLALMAGGVTDSFSLARGIHYLIRNQRKDGSWEEVRHTGTGFPRVFYLRY
                     HWYCQYFPLWALAMYRNLKTRGTTRADELRLQAYQSGQFRSPR"
     gene            5725..6480
                     /locus_tag="PK614_02025"
     CDS             5725..6480
                     /locus_tag="PK614_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81020.1"
                     /translation="MTAIGVFVATRWESAAVRQAFAASEMRVVGGIRCAVARQGQVEW
                     WVIPMGVGPERATATAGRVLGEQSFAAVWSTGFACALGQAVIGEVLIGTHVTMEGGAE
                     DGRSVPCAAAGADWVRQAVQQQRLPVQAGQFVSVPRVLCRAEDKQEIATRAGGVGLDM
                     ESAALGFVASERKIPFVIVRTASDLVDESLPLDFNLFLRPSGWVKGVAACLAHPTSLI
                     GLNRLRVQSRIAGTQLTAVFRACADKALREGLA"
     gene            6541..7308
                     /locus_tag="PK614_02030"
     CDS             6541..7308
                     /locus_tag="PK614_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB81021.1"
                     /translation="MEGIARIGRYTIDLTEQMGRMMLFVLSSFAWLTRPPFRVYQIVK
                     QLNFIGYKSTFVVVLTAVFTGMVLALQGHYTLRKFGSEAVLGSAVALSIIRELGPVLA
                     ALMVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIGPKLVASLIAVPLLVALF
                     DVVGIYGGYVVGVQLLNGNEGAYWSSIESAVEWKDVYGGILKSISFGLLISWVCCYKG
                     FHTKHSAEGLGTATTEAVVLSAVLILVWDYFLTSVLL"
     gene            7384..8145
                     /locus_tag="PK614_02035"
     CDS             7384..8145
                     /locus_tag="PK614_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81022.1"
                     /translation="MIKLVGVEKTLGKQPVLRGVDLTIPTGKLTTIIGRSGEGKSVLL
                     KHIIGLMQPDRGEVWIDGTNIARLKGQALNEVRKKFAMLFQGAALFDSMTVFENVAFP
                     LREKLRLKGEIVTRRVEEKLEQVGLKGMGHKFPAELSGGMRKRAGLARALVMEPEIIL
                     FDEPTTGLDPLMAKAIHDLIVAMQQQFKFTAVMVSHEIPEIFGISDYVAMLKNGRIAE
                     MAPSNEFVKTTDEEIREFIFVAGTVTPKGLPTASL"
     gene            8157..8600
                     /gene="mlaD"
                     /locus_tag="PK614_02040"
     CDS             8157..8600
                     /gene="mlaD"
                     /locus_tag="PK614_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRB81023.1"
                     /translation="MERAKLELMVGLFVLVGIACLGYLSIKLGKLEVIGGHNYPVEAE
                     FTSASGLKPGASVEIAGVEVGRVRNIGLSSDRALVALAIQDGVKLYSDTIASIKTRGI
                     IGDKYLALSVGGGGDPLKPGDKIRDTESGLDLEELVSQYVHGKVN"
     gene            8688..9302
                     /locus_tag="PK614_02045"
     CDS             8688..9302
                     /locus_tag="PK614_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB81024.1"
                     /translation="MHASRWMMIGALLALQIVVGGLSSAMAGPATDSVKGTIDEVLRI
                     LNDKELKAPARLEDRRQRLEKVVAQRFDYPEMSRRSLGAQWNQLSDKDKQEFIDLFRT
                     LLTNTYADRVETYSGEGVQYLNERTEKEYAEVRTKVLSGKTEIPMDYRLLNKSNDWHV
                     YDVVVDGVSLVNNYRGQFTKILHTSSYPELVDQLRKKSDKIKAP"
     gene            9337..10542
                     /locus_tag="PK614_02050"
     CDS             9337..10542
                     /locus_tag="PK614_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRB81025.1"
                     /translation="MQCHYLSSIRSCLALAGLLLLSLPAPAGADTQIFPVPSVSTSRN
                     DGNDAGLIAPILIADPDGELKYLMAPMLIQNSIVGTRGVFNLFKYDPGGRQMRFIASL
                     TERIERKVLFDYVDPAFGNGQYSLNFGGTFFKNATSRFFGLGQATTQAAESNYTAREA
                     RAYWRLGLYANEVTQISVGQRVRQVQLQRGAIDLPFSVEQFPTVDGIQGESIIVGHRA
                     SFYYDTRDSLVSPTDGVAITAYAELNQNVKNGDHPVYSRYELEVKKLFPSESKRAILV
                     VRADLQATIGSQVPFFEQSSLGGQNNLRGFGLDRYIDKHLIAFSIEERIHILRTKLAG
                     VTADFEVAPFLDTGQVFNSFKDVSFQDYRMTPGLGFRAIVRPNVVGRVDYGYSREGGA
                     VFAGLDFPY"
     gene            10573..11571
                     /locus_tag="PK614_02055"
     CDS             10573..11571
                     /locus_tag="PK614_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HRB81026.1"
                     /translation="MRYTIHCFCLLALLSVLPPAAGAEGFGPFPVRNFQALDQLVLAM
                     PGERATVLRKGDFDVRLEAANTASIARDSEAQAEVAMKFETIRAGLFLRYGLTDRLEL
                     GAEIPGYHRYRGFMEGAIIGVERGTTGVAPPRKALQAAGYAFNITNGARTLFQGSEGA
                     TGLGDISFYGKYQILKETSSLPALSVRVGVKAPTGDTGEVFGSGHPDAGIGLALDKKF
                     AGNWILYANLNGVFPTGRIAGLGLQPVVSGLVAVEYLWTENFSLTVQFDYYSPPFHGT
                     GTRVLDKGVTESVLGVSYRILPGLLWQLYGVENLDFITGSAADFTLSTVLTYRFRS"
     gene            11755..13701
                     /gene="dxs"
                     /locus_tag="PK614_02060"
     CDS             11755..13701
                     /gene="dxs"
                     /locus_tag="PK614_02060"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRB81027.1"
                     /translation="MSLLKNIHSPADLKRLSPEQFPELCQEIREQILTVVSNVGGHLA
                     SNLGVVELTVALQYLLDTPKDKIVWDTSNQAYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESVYDTFNAGHAGTGVSAAFGMVEAREQRNEKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGTNKDFIVVLNDNQMSISKNVGAISAYLNRTFTGEFYARMREETGQLLRKIPHIGLE
                     VQKIARRAEELAKGAILPGLLFEELGFQYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VITKKGLGYQAAMDNPVWFHACPPFVRETGVPAKKASRPSYTSMAIDALIKVAHQDKR
                     IVAVTAAMCEGTGLNAFEKVFPDRIYDVGIAEQHAVTFAAGMAAQGMKPVVALYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVVAAPKDE
                     NELQHMMKTCVTHDGPASVRYARGVSLGVPMDPEPTALPIGKGELLREGTDVAIVAIG
                     VTVWPAMKAAERLAQEGISAAVVNARFVKPLDTELIIKTAKNVRCLVTVEEGCKMGGF
                     GSAVLEALSEEGITNLRTKVIGLPDWYIEQGPQDLLRERYGLTADGIYNSVKALFGAS
                     VATDDAARLASLVGSLPHGDEQGS"
     gene            13746..14951
                     /gene="ispG"
                     /locus_tag="PK614_02065"
                     /gene_synonym="gcpE"
     CDS             13746..14951
                     /gene="ispG"
                     /locus_tag="PK614_02065"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRB81028.1"
                     /translation="MHITRKKTRQIQAGSVKIGGDAPISVQSMCSTDTRDVNATVAQI
                     RQLEEAGCEIIRVAVPDEAAAAALPQIKAAMTVPLIADIHFDHRLALKAAEVVDCVRI
                     NPGNIGAWWKVQEVIKAVNERGIPLRVGVNGGSLERPLLDKYGWPSPEALSESALNAV
                     HALEDEGFTNMKVSLKASDVHHAIDAYYLFSTQSNYPLHIGITEAGTAMTGAVKSAIG
                     LGWLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLMRKV
                     PLEELMDTLIHEVELLAKEKEAEAAGVTHETAASPDGQAEPGWELIGHAPDEHSTIVR
                     DIPVLPTKR"
     gene            15052..15127
                     /locus_tag="PK614_02070"
     tRNA            15052..15127
                     /locus_tag="PK614_02070"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15085..15087,aa:Cys,seq:gca)
     gene            15674..17419
                     /locus_tag="PK614_02075"
     CDS             15674..17419
                     /locus_tag="PK614_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRB81029.1"
                     /translation="MAPDPIFFRDLAYVFIAAVIGGASAYLLRQPLILGYVFGGILIS
                     PFTPGPSISDVHSFELFAEIGVILLMFCIGIEFSLNDLLRVRWVALLGGPLGIFLSIF
                     LAVGLGHVVGWSGIQSVVIGSVVSVASTMVLARLLLDSGDLRAKHGRVMIGITLVEDL
                     AVVVLTVLLPALGALEPGRFATIGKALWLAVLILVPFAYLAAKAIPPLLTRIAKTQNA
                     ELFLLVALSLGIGTAAVTQMAGLSLALGAFLAGLIISGSEYGHETLARLLSLRDAFVA
                     LFFVTIGALIDPRVVWANLPLLGLMLGLIIVGKFVIWTLVVKLFRYAWGTALLVGAGL
                     TQIGEFSFVLVQVAKTAGHVSEDVYNATLAASLLSILLNAPLVRYAPGWIAALHFKMG
                     RVSLPIQGGISDEQAGHVVLCGFGRMGSTVGLALDHFELPYTVIERDPDIVRQLRRRG
                     ISCVYGDASQTELLKAAGAQHAALVIVALPVIHETSLTVRRFRGLNEKIPLLARAHGF
                     REAEDLKDVGATEVILPEVEGAHTLIRHAFQALKVSKPSILDYLKSCQAFRSSGEGPD
                     SGNVEAVKASVGRST"
     gene            complement(17610..18098)
                     /locus_tag="PK614_02080"
     CDS             complement(17610..18098)
                     /locus_tag="PK614_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81030.1"
                     /translation="MNGRNVAHSSLWVLLISGFFGCAGAGPFVTLPSSAFFETDVKER
                     EALQVVSRTQEARRANCQQNASCEEVTYLRGLVALFENREDAIHAFQELRTTVPNGRY
                     AASSTRWLTLLQGGQPASVRHEPLFMQLRQEILHGLLDPDTLAASRKLKEQERRIAEV
                     SR"
     gene            complement(18620..19243)
                     /locus_tag="PK614_02085"
     CDS             complement(18620..19243)
                     /locus_tag="PK614_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB81031.1"
                     /translation="MGRWLVAFLFLVMLGFPARTPAETYVAAGVGMNAPTFDTTDAGK
                     VSLQKDLFYGGKIGHYFNDRGYNWFGLEVDAYRSTPGIKQQTLPTSSNPRLSGTIPGA
                     DLMVHSLAFNALVRVTGYQYKVEPYAGLGIGLNVGNISSGGFRPEASFAPSFNVLAGI
                     RYYMTDSIAPFVEYKYNFAQFTFNRSDITADYRANLFMFGIAYHFGR"
     gene            complement(19455..21581)
                     /locus_tag="PK614_02090"
     CDS             complement(19455..21581)
                     /locus_tag="PK614_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81032.1"
                     /translation="MRFSTTTFLLNLEKQGLEDNTFTNGLGRDTTLIGNLVNASIYKR
                     LLPTLEAELGVFANMPFGHDHEVSQVRPIMRLTYQPNDAVTGVIGTLYVPHQDFHNAV
                     FYRGNRFLRPIEQGAQMLVDSTHYRQDLFINWEQAFRGSAPNRYDVGYAGQLKFGPLR
                     FNGQAHWVRNGQALFKLDRSFQAGDNLVVAAGPELALEPFRYFPALPWWNQLGIRFTY
                     LTSYNQTDAGAEATRGRGYELHAWLDLAGWRPYLAFWKGRNFFSQQGDPEFRASNFPE
                     IGLSKTVRLGDRTSIEFGAQARRIRAFFTEDGITYQNRDTFKWVNQEYLVFNWNWDTN
                     HADLIGDLWASMSRPFGDRQDASSERRFSAKLDALTYVYNIAYPGVRQIGGVPVSNQT
                     FAGQYLSPVLQYRVSPQLTLNAGFFTGLPVGDTQRFHAVQPILSAEYEMVPDMRVVAG
                     TLHRNHPFVDALFNDAMLFVRPIEQGFQLLVAREHYQQDLFINWSQVETFQKAERFDV
                     GYAGRVSAGHFSLNGQVYWSHSGGAQYSESRTFFGPGIPRDRPAGNNFMTAFGPQFTF
                     EPRQYWSQLSWFREIDVMALYLTSQNEPTQGGSPIVRGRGYQLTAGLNLDGWRPYVTL
                     WRGEHFVTERGDPAYYAGDFTEFGLVKDMALPAGFSLRIGGFGRTIEQRITHTEYALL
                     NWSWDQSRWRGFCLRPTLLHREETQC"
     gene            21823..>22736
                     /locus_tag="PK614_02095"
     CDS             21823..>22736
                     /locus_tag="PK614_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRB81033.1"
                     /translation="MPWGSKSLRKNLSWALNGAIFYVFSQWVLLIILAKLGGPTIVGR
                     YTLGLAMTAPIVLLSNLQLRTLLASDVQEMFPFSAYAILRLLTSLGALLLICGLAACL
                     EFTLDQAVVICLIGLAKVIESGSDITYGLMQKHERMDLVSLSKGIRGILTAGAVGGIF
                     WGTNSLIAGVGGMIAVWLMVFVLFDCRHLQRILGNPPSHMAWAKMVHANLASKILSLS
                     KFSLSLGMVSMLMSYNTAMPRYLLEAYHGEAALGYFSAIACFTVACTIVPEAFSQAAL
                     PRLAKYHANSLDSYWRLMGKLMLVAFLIG"
BASE COUNT         4537 a         6078 c         7266 g         4855 t
ORIGIN      
        1 attgaaacca gggcggggtc caagggccat accgtcatcg agcaggggcg gattgagcag
       61 atcctgcagg cctcgccgca ggatcgccgg gagctgatcg aggaaaccgc cggtatcgtc
      121 cgctacaaaa aacaaaaggc cgaagccctc cgcaaacttg aggcgacgca gcagaatctg
      181 gtgcgggtgc gcgacatcgt ggccgaggtc aaaaaacagc tgaattcgct ggagcgtcag
      241 gcccgccagg cccgttccta tcagacgttg cagcaggagg cccgcggtct cgaaatcgaa
      301 ctcctgtccc gcgactatcg caccatgcat gccgacctgg agtccgtcga tcacgaagcg
      361 cgcgaggtgg aagcgcagga agcggaacag gtggccgaac aagcccgcct cgactctgag
      421 caggaaacga tcaggctgcg catgaacgac gcggcggagg ccatttcgcg tgcacgtgac
      481 acgttggccg gcaccgaaca gcggcagtcg caggccttga cggccgcgga ggtcgaacgg
      541 aaccgcacgg aattgtttga acgtcaacgc atgcaggccg cgcaagagat ggaacggttg
      601 gcggcggatc aggagcaggc gcagtcggaa atcgagaccc ttcgggcgat gctgcttcaa
      661 ttggaggggg acatcgtcga acaggaagcg caattccagc aggcggatgt cgaggcgaaa
      721 tcgttggccg ggcatcgcgc gtctgcggtg gcggaggaag agcgcgcgcg gaaagatgtg
      781 ttgaacctgg ccgtccttgt ggccaataca gagcagagcc tgacgcaggt gacggcccgt
      841 cagcaggaaa ccgccgcccg ggcggaacgg gtctcgcgcg aacaggaaca gttggtggca
      901 caggtggccg ggttggatca gcagcgcgac ttgctggccg cgcaacggga agaggccagc
      961 gggcgcattc aagagctcat ggccgaacgg caggcggcga tcgagcggat cgaaggactg
     1021 ggcgggcaga tcaccgggtt ggaccgcgat atcgtgaagt tttccgaaga cgtcgccggc
     1081 gtggaatcgc gtctcggtgc cctgcagggt gtcgtgcgtg aggaaatggg atatggccgg
     1141 gaaggtgagg aggaaggcac ggcgttgaaa acgtgcgacg gcgtgcgcga ggcactcgcc
     1201 gagtggctcg tgattccgcc ggggctggac cgggccgtag agacgatcct cggggagcgt
     1261 gttcgcggct ggctggtgga cgagccgtcg gcggcttgtc gcgcggtgga gtttttgaag
     1321 ggcaaagaac tggggcgcgg cgcctttctt ccgcagcagt tgcgatgggc gcctgagcag
     1381 gcggcgcttg attcatcggc ctcgtggtgg ccgacgctga acgggcaggc gggcgtggtg
     1441 gggcgtgcga cggatttgat ccgcgcggaa ggcgcctctg ccgctacctt gagctatttg
     1501 ttcgacggga ttgtgtttgt ggagtcactg gacgtggcgc tgcagctttg gcagcagcat
     1561 caatgggctg ctcccgccgg gccaacatat gtcacgttat ccggtgaaac gctggacgcc
     1621 gccggtgtga tgaccggggg cggcagcagt gccagtgggg gcttgctcca acgtcggcgc
     1681 gaagtattgg agctggaagc ccggaggact gcagcggttc aagcccttga gcaggcacgg
     1741 tcggcgcggg aggacgcggc ggccgagttg gggctcggac gtgaagatga gcagcgcctc
     1801 ggccgagcga tccgcgaagc agagatgctg gagttgtcgc tgcagaaaga cgatgcgggc
     1861 gtggaacgcc agcgcgggga attgcaccgg cggatggagg tgttggcgga ggagctgcag
     1921 cggggcttgg ccgagcaggc gcggcttcag gaagagtttc agtccagcca ggcgcagttg
     1981 gggcaatggg tggcggaaaa ggtcgggcag gaaaccgggt tgctgcacgt gcgagaacgg
     2041 ttggtggtga tcgaaagcca gagccttgcg atccagcaac gattgaccga agtccggttg
     2101 tcgctggaga gcatgcgcgg gcgccgggag catgccacga acgacatcgc acgatttaca
     2161 cagcgcctgg atgccgcgca acgccgggcg accgatttgg aagagcaggt ggccgggttg
     2221 gttgaagcga cggagaatag tcgcgaagag cagaccaggc aggaagcatt gtgtcgggaa
     2281 ttgggcgctg aggtagacgg aatcaaggcg gagttagtgg cggcccagga gcggcaggca
     2341 caggaaatgg ccgggttgca tgcggtggag gaaactctta gccaggtacg gcagagcctc
     2401 tccgccttgc atgatcgacg gatgagggcg gaagtgcgaa aggcagaagt gaaggcgcat
     2461 ctctccacga ttgagagcac gctcgccggc acctatcaga tcgagccggc tacgctgctc
     2521 ctgccggcga gtgatcagcc ggtaccggag ggtgaagaac cggcggcccc cgtctcgatg
     2581 cttgaaacgc cgcaattgcg ggagcagatt cagaaaatcc gtgagcgcct ggaccggatg
     2641 ggcgccatca atctggcggc catcgacgag catcgtgagt tggaagcgcg gtatcagttc
     2701 ctcacgacgc aggagcagga cctctcgacc tccatcgcgt cgctcaagga gatcatccag
     2761 cggattaacc ggacgacgaa ggacatgttc gtggagacgt tcaacgagtt gcagcagaaa
     2821 ttccgggacg tcttttccca gttcttcccc ggaggtcgcg ctgaattgca actggtcgaa
     2881 gagccgttgg aagagggcgt ggaagacaac ggggcgcgcg agcccggtgt ggagattgtg
     2941 gcccagccgc cggggaagcg cttgaagagc attaccatgt tgtccggcgg agagaagacg
     3001 ctgaccgcga tggcgctgct catcgccagc ttcctgatcc gcccgacgcc gttctgcatc
     3061 ctggacgaaa tcgatgcgcc gctcgacgaa gaaaatatcg ggcgcttcac gagcgtgttg
     3121 cgcagcttgt cgtcgaccgc tcagttcatg gtcatcacgc acaataagcg gacgatggcg
     3181 atggcggatt cgctgttcgg ggtgacgatg gaagagcccg gtgtgtcgac gttgatttca
     3241 gtcaagctcg gtgatctcca gccggtgtag tcagagcgga gatgagtacg taggggctgt
     3301 tccggcgggt ggtgaaggca atgccgccgc gcgagggggg ccgcttccct tgactgccta
     3361 gaaacagttt gttatacagt tctgctaatg gttataagtg gtactgtgcg gtttcatgtg
     3421 atcgcttcct gatcgatgga accgtctcga ctatcaatcc cgccgtcttt ttcagattca
     3481 cggcatcacg ttatgcgact tctcaaaaac ctgttcaatc gtttgtccga tagtttgctg
     3541 gtgtcggtgc cgagccgaat cagggcggcg cgcgactttg cctccgctcc ggtgctccga
     3601 ctcgtctcca ataagtctgc ggtgccggct tccggcgaca gtgccgcgaa gcgagccccg
     3661 gcacattcca caggccaggt ggaagcgctg gaagaggcgc tgcggaaaag ccaggcctgg
     3721 tttctggcgc gacaagacgc gtccgagggg tactgggttg ccgagttgga agccgatacc
     3781 acgctgacat ctgagtatct gatgttgcgc cgcttcctgg actgcatcga tcccgagcgc
     3841 gagcgcaaag cggtccgcta cttaaaatcc gctcagttgc cagacggcgg atggtcgatt
     3901 tatcacggtg gcccggcgga gatcagcgcc tcggtgaagg cctactttgc gttgaagctc
     3961 tccggcgtgt ccgccgacga gcccttcatg gtgaatgccc gcacctgcat cctggacaag
     4021 ggtggagtgg tggcggcaaa tgtcttcacg aaaatcgccc tggccctgtt cggtcaatat
     4081 gactggcgtg gtgtccccag catgcctccg gaaatcatgc tgttgccgaa gcggttctat
     4141 ttcagcattt acgcgatttc ctactggtct cgtgcggtgc tgattccgct gttgatcata
     4201 ttcgccaaac ggcccttgtg tcatgttccg ccggaactgg gcatcgacga actctatacg
     4261 gagcccccgg cagagatcga ttacggcatg gttcctccgt tcaagaagga ccggacctgg
     4321 ttcacggcta ggaacttctt catcaatctc gatgcgctcc tcaagatcta cgatcgttcg
     4381 cccgttgagt gggttcgaca gaaggcgctc aagtgcgctg agaactggat gctcgaccac
     4441 atgaagggca gcggaggcct cggcgcgatt tatcctgcca tggccaattc cgtgatggcg
     4501 ctgcattgcc tcggctacaa aaacgatgat ccccttatgg tgaaggccat ccgggagatc
     4561 gaagaactgg aagtacacga cacggtgcag gacaacggcc aatgcgtcga tgcgatgcat
     4621 ttgcagccct gccactcccc gatctgggac acggccttgc tcatcaatgc cttgatcgag
     4681 gccggtatgc cgcaagatca tccggcgctg cagaaggctt cgacctggtt attgtccaag
     4741 cagactaaga ccgtcggcga ttggattatt tcggctcccg gcgccgaacc gggcggatgg
     4801 tatttccaat tcgagaacga actgtttccc gatgtggacg attccgcggt cgtgctgatg
     4861 gccctggcga aggtgcatct gtccgatgag gcccaacaac gtctggcgat tcgtcgcggt
     4921 tgccgctggg tgacctctat gcagggatcg gacggaggct ggggcgccta cgacgtcgac
     4981 aataatcgga tcgtgtttaa ctacattccg ttcgccgacc atcgggccct gctcgatccc
     5041 agcacggcag accttgccgg acgctgtctg gagatgcttg cgacgctggg atacgactgg
     5101 acccatcctg ccgtggcttc tgcgctcacg tttgtgaaaa acgatcagga gcaggacggc
     5161 agttggtatg gtcgttgggg cgtgaattac atttatggca cctggtcggt cctgtccggc
     5221 ctgcgagcga tcggcgagga tctctcgtct ccctatatcc gccgggcggt cagttgggtc
     5281 gagtccaagc aaaaccccga tggcggatgg ggggaatcct gcctctcgta tggagatgtc
     5341 tcgcagagcg gtcgcggtga cagcacgccg tcgcaaactg cctgggcgct gttggccctg
     5401 atggcaggcg gagtgacgga ctccttcagc ctggccaggg ggatccatta cctcattcgg
     5461 aatcagcgga aggatgggtc atgggaagag gtgcgccata ccggaactgg tttcccgcgc
     5521 gtgttttacc ttcgttatca ttggtattgc caatacttcc ccctctgggc gctggccatg
     5581 taccgcaatc tcaagactcg tggaacgacg agggccgatg aattgcgtct gcaggcctat
     5641 caatcaggac aattccggtc gccacgctga ccgtgggtgt tcatcgagtt cagcttttcc
     5701 tgtcattgtc cctcgggagt cattgtgacc gcgatcggag tcttcgtggc aacccgatgg
     5761 gagtcggccg ctgtgcgtca ggcgtttgcc gcgagcgaga tgcgggtcgt cggagggatt
     5821 cgttgtgccg ttgcgcggca gggtcaggtc gagtggtggg tcattccgat gggtgtcgga
     5881 cccgagcggg ccaccgcgac cgccggacgg gtgctcgggg aacagtcatt tgccgctgtg
     5941 tggtccacgg ggtttgcctg tgccttgggt caggccgtta tcggcgaggt gttgatcgga
     6001 actcacgtga ccatggaagg tggcgcggag gacgggcggt ccgttccctg tgctgcggcc
     6061 ggagccgatt gggtgcggca ggccgtgcag caacagcgcc tcccggtgca ggccgggcag
     6121 ttcgtttcgg tgccgcgggt tctctgccgg gcggaggaca agcaggagat tgccactcga
     6181 gcgggagggg tcggcctcga catggagagt gcggcgttgg ggtttgtggc ttcggagcgc
     6241 aagattccat ttgtcattgt tcgtacggcg tcggatcttg tcgatgaaag ccttccgctt
     6301 gacttcaatc tgtttctcag gccatctgga tgggtgaagg gtgtcgccgc ttgtctggct
     6361 catccgacga gtctcatcgg actcaatcga cttcgtgtgc aaagccgcat cgccggaaca
     6421 caattgacgg cggtattcag agcctgcgcg gataaggcgt tacgcgaggg actggcctga
     6481 ccgggcccaa cgtgtttcga cggctgagtt tcgggtaggt ggacgagagg agagcgtggc
     6541 atggaaggca ttgctcgtat cggacggtat acgatcgatc tgacagagca gatgggacgg
     6601 atgatgttgt ttgtcctctc ctcctttgcc tggctgacgc gtccgccgtt ccgcgtttac
     6661 caaatcgtca agcagctgaa tttcatcggc tacaagtcca cgtttgtcgt cgtcctcact
     6721 gccgttttta ccggcatggt cctggccctg caggggcatt acacgctacg aaaattcggt
     6781 tctgaagcgg tgctcggctc tgccgtggcg ctcagcatca tccgggaatt gggtcccgtt
     6841 ctggcggcgt tgatggtgac ggcccgggcc ggatccgcca tgaccgcgga aatcggcatc
     6901 atgcggatta cggaacagat cgatgctctg gacacgatgg ccatcaatcc actgcaatat
     6961 ctgatcgggc ctaaactcgt cgccagtctc atcgccgtgc cgctgctggt cgcgctcttt
     7021 gacgtcgtcg ggatttatgg ggggtatgtc gtcggcgtgc aactgttgaa cgggaatgaa
     7081 ggcgcctact ggagttcgat tgagtcggcc gtcgagtgga aggatgtcta tggaggcatt
     7141 ttgaaatcca tcagcttcgg cctgctgatc agttgggtct gttgttacaa aggctttcac
     7201 accaagcata gtgccgaggg gttggggacc gcgacgaccg aagcagtggt gctctcggcg
     7261 gtcttgattc tggtgtggga ttattttctg acgtcggtgc tgctctaaca tcgtgaaacg
     7321 tgaagcgcat gaggagaatg cggctgcggt ggaacggggc agctgccgcc cgcgcgaggc
     7381 tccatgatta aactggtcgg cgtcgaaaag actttgggca agcagccggt gttgcggggc
     7441 gtggacctga ccattcccac tggaaagttg acgacgatca tcgggcgaag cggcgaaggc
     7501 aagagcgtgc tgctgaagca tatcatcggc cttatgcagc cggatcgcgg cgaggtctgg
     7561 atcgacggga cgaatattgc gcgtctcaag gggcaggcgc tcaatgaggt gcgcaagaag
     7621 ttcgccatgt tgttccaggg tgcggccctg ttcgactcga tgacggtatt tgaaaatgtc
     7681 gccttccccc tgcgagaaaa gcttcgcctg aagggcgaga tcgtcacccg gagggttgag
     7741 gagaaactgg agcaggtggg actgaagggc atgggccata agtttcccgc cgaactgagc
     7801 ggaggcatgc gcaaacgcgc cgggttggcg cgcgccctgg tcatggagcc ggagatcatt
     7861 ctgttcgacg aaccgacgac ggggctcgat ccgttgatgg cgaaagccat tcatgatctg
     7921 atcgtggcga tgcagcaaca attcaagttt acggctgtca tggtcagtca cgagatcccc
     7981 gagattttcg ggatttccga ctatgtggcg atgctcaaga acggacggat cgccgaaatg
     8041 gcgccatcga acgagtttgt gaagacgacg gacgaagaga ttcgagagtt tatttttgtc
     8101 gccgggaccg tcacgccgaa gggattgccg accgcatccc tctaatagga gacgttatgg
     8161 aacgcgcaaa gctggaattg atggtgggac tctttgtgct cgtcggaatc gcctgtctag
     8221 gctacctgtc catcaagttg ggaaaactgg aagtcatcgg cgggcacaat tacccggtcg
     8281 aggcggagtt tacctccgcc tcgggactca agccgggcgc ctcggtggaa atcgcggggg
     8341 tcgaggtggg ccgcgtcagg aacatcggtc tgagcagtga ccgtgccctg gtggcattgg
     8401 ccattcaaga cggcgtgaag ttgtattccg atacgatcgc ctccatcaag actcgcggga
     8461 ttatcgggga taagtatctg gccctctcgg tgggcggcgg cggcgatccg ttaaagccag
     8521 gtgacaagat tcgtgatacc gagtccggac ttgatctcga agaattggtc agccagtatg
     8581 tacacgggaa ggtcaactag gctgatcgtg tcagctgtca ggaaatgaaa gtgaacaagc
     8641 caggccggtg gggtcaggga ctcaccactc aaaggagagg tgttggaatg catgcaagcc
     8701 gttggatgat gatcggggcc ttgctggccc tgcagatagt ggtgggcggg ctttcgtccg
     8761 ccatggccgg accggcgact gattctgtta agggcaccat cgacgaggtg ctcaggatct
     8821 tgaacgataa agaattgaaa gccccggccc gcctggagga ccggcggcag cgcttggaaa
     8881 aagtggtggc ccagcggttc gactatccag aaatgtcccg gcggtcgttg ggtgcacagt
     8941 ggaatcagtt gtccgacaag gataagcaag agttcatcga tctgttccgg acgctcttga
     9001 ccaatacgta cgctgatcgt gtggagacct attcaggcga gggtgtgcag tatctgaatg
     9061 agcggaccga gaaagaatat gcggaagtcc gcaccaaggt gctctccggg aaaactgaaa
     9121 tccccatgga ttaccggttg ctgaacaaga gcaacgattg gcatgtgtac gatgtcgtgg
     9181 tcgatggcgt cagtctggtg aacaactacc gcggacaatt tacaaagatc ctccacacct
     9241 cctcgtatcc cgaactcgtc gatcaactcc gcaagaaatc cgacaagatc aaagctccgt
     9301 agctccggta ggaagcgagc gattccggcg agcgccatgc agtgtcacta tttgtcgtcg
     9361 attcgaagct gtctggcgct ggccggcctt ctgctcctga gccttccggc tccggccggc
     9421 gccgacacgc agatttttcc ggttccctcc gtctcgacca gccggaacga tggcaacgac
     9481 gccggtttga tcgcgccgat cctgattgcg gatccggacg gagaactgaa gtatctgatg
     9541 gcgcccatgc tcattcagaa ctcgatcgtg ggtacacgcg gggtcttcaa tctgttcaag
     9601 tacgatccgg gtggacgaca gatgcgattc atcgcctcgt tgaccgaacg gatcgaacgc
     9661 aaggtgttgt tcgattatgt cgatccggca ttcggcaacg ggcaatattc actcaatttc
     9721 ggcggcacct tttttaagaa cgcgacctca cggttcttcg gcctcggcca ggcgacgacc
     9781 caggccgccg agtccaacta tacggccagg gaagcgcgcg cctactggcg gctcggcctc
     9841 tacgcgaatg aagtgacgca gatttctgtc gggcagcggg ttcggcaggt gcagctccag
     9901 cgaggagcga tcgatctgcc cttttcggtc gaacaatttc ccacggttga cggtattcaa
     9961 ggcgagtcca tcatcgtggg ccaccgcgcc tcgttttatt acgatacccg tgacagcttg
    10021 gtttccccga ccgacggcgt ggccatcacc gcctatgccg aactgaatca aaatgtgaaa
    10081 aacggcgacc acccggtcta ttcgcggtac gaactcgaag tcaaaaaatt attccccagc
    10141 gagtccaagc gcgccattct ggtggttcgc gccgacctgc aggcgacgat cggatctcag
    10201 gtgccgtttt tcgaacagtc ttcgctcggg gggcagaaca atctgcgcgg gttcggcttg
    10261 gaccggtaca tcgacaaaca tctgattgcc tttagcatcg aagagcgaat tcacatcttg
    10321 aggacgaaac tggccggggt gactgcggat ttcgaggtgg cgccgtttct cgacacgggg
    10381 caggtcttca actcattcaa agacgtcagc tttcaagatt atcgaatgac gccggggttg
    10441 gggtttcgcg ccatcgttcg tcccaatgtc gtcggccggg tcgattacgg gtacagtcgc
    10501 gagggcggag cggtcttcgc agggttagac tttccctatt aagtgccact gagtgaacgg
    10561 ccggggttac tcttgcggta caccattcat tgtttctgcc tccttgcact cctgagtgtc
    10621 cttccgcctg ctgccggagc ggaaggcttc ggtccgtttc cagtcagaaa ctttcaagcg
    10681 ctggatcaat tggtcctggc catgccgggc gagcgggcga cggtgcttcg caagggcgat
    10741 ttcgatgtgc ggctggaagc ggcgaatacg gccagcattg ccagggacag tgaagcgcag
    10801 gccgaggtgg cgatgaagtt tgaaacgatc cgggccggcc tgttcctccg gtatggtctc
    10861 accgatcgat tggagctcgg cgcagagatt cccggctacc atcggtaccg gggcttcatg
    10921 gagggggcga tcatcggggt ggaacggggc acgaccggcg tggcgccgcc gcgcaaggct
    10981 ctgcaggcgg ccgggtacgc gtttaatatt acgaatggcg cccgcacgct gtttcaggga
    11041 tccgaaggcg cgactggatt gggagacatc tcgttctacg gcaagtacca gattctcaaa
    11101 gaaacctcga gccttccagc cctgtcagtc cgggtcgggg tcaaggctcc gaccggagac
    11161 accggcgaag tgttcggcag cgggcatcct gacgccggca tcggcttggc gctggataag
    11221 aagttcgcgg gtaactggat cctgtacgcg aatctcaacg gggtatttcc tacgggacgg
    11281 attgccgggt tgggactcca gccggtggtg agcgggttgg tcgccgtgga atatctctgg
    11341 acggagaact tttccctcac ggtacagttc gattattatt ccccgccgtt tcacgggacc
    11401 ggaacgcgcg tcctggataa gggcgtgacg gagtcggtgc tgggggtgag ttatcgaatc
    11461 cttcccggtc tgctctggca gctgtatgga gtggagaatc tggatttcat caccgggagc
    11521 gcggcggatt ttaccctctc caccgtcctc acctaccggt tccggtcgta gtcgcgaggg
    11581 cgaagttttt ggtgcagggg aaggcgaaat cttgacacag gcagtgccat atgtgaggat
    11641 gagccgatta tacgaaactg cttcgctgct tcgtcgcgac cttctctata cggcttgttc
    11701 ccactgaatg aatacctgcg cccaattgtc atcatttgac aaggagattg tgcaatgtct
    11761 ctgctgaaaa atatccacag tcccgctgat ttgaaacgcc tctcccctga gcagtttccg
    11821 gaactgtgcc aggagatccg tgagcagatc cttacggtgg tttcgaatgt cggggggcac
    11881 ctggcctcca atctgggtgt ggtggagctg acggtcgcgc tgcaatatct cctggatacg
    11941 ccgaaagaca agattgtgtg ggataccagc aatcaggcct atacccacaa attgctgacc
    12001 ggacgccggg agcagttcca tacgctccgg cagtacggcg ggttgagcgg gttctgtaag
    12061 cgcgaggaga gtgtctacga taccttcaat gccgggcatg ccggcaccgg agtctccgcc
    12121 gcattcggca tggtggaggc gcgcgagcag cggaacgaaa aacacaaagt ggtctgcgtg
    12181 gtgggtgacg gcgccatgac ggccgggatg accctcgaag ggttgcacca tgccggaggc
    12241 accaacaagg atttcatcgt cgtcctgaac gacaaccaga tgtccatctc gaagaatgtc
    12301 ggggcgattt cggcctacct gaaccggacc ttcaccggcg aattctacgc gcgtatgcgg
    12361 gaagaaaccg gccaactgtt gcggaagatt ccccacatcg gccttgaggt gcagaaaatc
    12421 gcccgacggg ctgaggaatt ggccaagggg gcgattctcc cgggtctgtt gtttgaggag
    12481 ttgggattcc agtatgccgg accgatcgat ggacataatt tcgagcacct gctgccgacc
    12541 ttggagaacg tgctcaaaat gaagggcccg gtcctgctgc atgtgatcac gaagaagggc
    12601 ttgggctatc aggcggccat ggataatccg gtctggttcc atgcctgtcc gccctttgtg
    12661 cgtgaaacgg gtgtgccggc caagaaagca tcgcgcccca gctatacgag tatggcgatc
    12721 gatgcgctga tcaaagtcgc tcatcaggac aagcggatcg tggccgttac cgccgccatg
    12781 tgcgagggca cgggcctcaa cgcatttgaa aaggtgttcc cggatcggat ttatgatgtg
    12841 ggcatcgccg agcaacatgc cgtcaccttc gccgccggta tggctgcgca gggcatgaag
    12901 ccggtggtcg cgctctattc cacattcttg cagcgggcct atgaccaggt cgtgcacgat
    12961 gtggctactc agaacctgcc ggtgaccttc tgtatcgacc ggggcggtct tgtggccgag
    13021 gatggcacga cccaccatgg cgcatttgac tttgccttcc tgcggcatgt gccgaatatg
    13081 gtggtggcag caccgaagga cgaaaacgag ctgcaacaca tgatgaagac ctgtgtgacg
    13141 catgacgggc ccgcctcggt gcggtacgcg cgtggcgtca gtctcggggt gccgatggat
    13201 cccgaaccga cggcgttacc gatcggcaaa ggcgagttgt tgcgggaagg cacggatgtg
    13261 gcgattgtgg cgatcggagt caccgtctgg cccgccatga aggcggctga acggttggcg
    13321 caggagggga tctccgccgc ggtggtcaac gcccgtttcg tgaagccctt ggacacagag
    13381 ctgatcatca agacggcgaa gaacgtgcgc tgcctggtga ccgtcgaaga aggctgcaag
    13441 atgggcgggt tcggttccgc tgtcttggaa gccctgtcgg aagaaggcat tacgaacctg
    13501 agaacgaagg tgatcggtct tcccgattgg tatatcgagc agggtccgca agacctgctg
    13561 cgcgaacggt atggcctgac ggccgacgga atttataaca gcgtgaaggc gttgttcgga
    13621 gccagtgtgg ctaccgatga tgccgccagg ttggcgtcgc tggtggggag tttgccgcac
    13681 ggcgatgagc agggtagcta gttgacgtcg gggcgcacca aaacaggaca tacgctgaga
    13741 agctgatgca tatcacgaga aaaaagacgc ggcagattca ggcgggatcg gtcaagatcg
    13801 gcggagatgc gccgatttca gtccagtcga tgtgctcgac ggatacacgc gatgtgaacg
    13861 ccacggtggc tcagatccgt cagctggaag aggccgggtg tgaaatcatt cgtgtcgccg
    13921 tccctgatga ggcggcggca gctgcgcttc ctcagatcaa agccgcgatg acggttccgc
    13981 tgatcgccga cattcacttt gaccatcgct tagccctcaa ggcggcggag gtagtggatt
    14041 gcgtccgcat caatccaggc aacatcggcg cttggtggaa ggtgcaggaa gtcattaagg
    14101 ccgtcaatga gcggggcatt cccttgcgcg tgggcgtaaa tggcggctcg ctcgaacgtc
    14161 cgctgttgga caagtatggc tggccctctc ccgaagccct gtcggagtcg gcgttgaacg
    14221 ccgttcatgc cttggaagac gagggcttca ccaatatgaa ggtgtcgctc aaggcttccg
    14281 acgtgcacca tgccatcgat gcctactatt tgttctccac ccagtccaat tatcccctgc
    14341 atatcggaat cacagaagcc ggtacggcca tgacgggcgc ggtgaagtcc gcgatcggcc
    14401 ttggctggct cctgtcgcaa gggatcggtg ataccctccg agtctcattg gcggcggatc
    14461 cggtggagga agtcaaagtc gggttcgaga tcctcaagtc gctggagttg cgccatcgtg
    14521 gcatcaatgt gattgcctgc ccgacctgcg gccgggttga gatcgatgtg gtgcgcatgg
    14581 cgaatgaact ggaaaagaag ctcggccata tcaagacgcc gctgaacgtc tccgtgctcg
    14641 gctgtgtcgt gaacggcatc ggggaaggca aggaagcgga tatcggcatc gccggcgggg
    14701 aaggcaaagg gattctcttc aagaagggca aattgatgcg gaaagtgccg cttgaagaac
    14761 tcatggacac gttgatccac gaggtcgagt tgctggctaa ggaaaaagaa gccgaagccg
    14821 ccggcgtgac tcacgaaacg gcagcatcgc cagatgggca ggcggagcct gggtgggaat
    14881 taatcggcca tgcgccggat gaacattcga ctatcgtccg cgatattcct gtgcttccca
    14941 ccaagcggta ggccaccgat ttccagccca acagtgtagt cccaaccgct ctactagggc
    15001 cgttttgccg gttgtgttcc agccctgctc ccttctataa tcagcccccg tggcgccgta
    15061 cccaagtggc taagggagca gtctgcaaaa ctgcgatgcg gcggttcgaa cccgcccggc
    15121 gcctccatac cctgcttgac ccgaccgcga gcctatgtat cgcgtgtttg tgattgaggc
    15181 gagcggataa actcttccag gtcattcggc aggaaatagt tctcgccctt gtcatctcct
    15241 ttgtatccga ctgctcccgg ttttacttcc ggttccgttc agacttgtcg ctgtgatccc
    15301 tttccaacct atgtacaagg cggcgatgcg tccgatccca ctcccagtgt tgctcccgtt
    15361 tcgatatcct agacagtcgc agtagggtgc aatcgcgagc ggtagatcat tgattgtctg
    15421 gtcgacctct ctctatccgt cgttcttcgc cgtcgtcggt cgtagagccc tcgggcaagc
    15481 tgcaaaactc cactgtagga aaataaaacg ggggaacctt cgccatatca caccgctagt
    15541 agccgacacc cgccgaacat gcaatgcgcg cgtccttatc aaacaatcct ttaacatctt
    15601 acttctcgcg tcaaccagcg ttggttcatg ttagtctgca gcctggagga cgaccttcta
    15661 cctgagagga ctcatggccc ctgatcctat ttttttcaga gatctcgcct atgtctttat
    15721 cgctgcggtc ataggcgggg cctccgccta tctgcttcgt caaccgttga ttctgggata
    15781 tgtcttcggc ggcattctga tcagcccgtt tacccccggt ccctccattt cggatgtgca
    15841 tagtttcgaa ttgttcgccg agatcggcgt gatcctcttg atgttctgta tcgggatcga
    15901 attttccctg aatgacttgt tacgggtccg gtgggtcgcc ttgctgggag ggccactcgg
    15961 cattttcctc tccatttttc tcgccgtcgg cctgggccat gtggtgggat ggagtgggat
    16021 tcaaagcgtg gtgatcgggt cggtggtgtc cgtggcaagc acgatggttc tggcgcgctt
    16081 gctgctggac agcggagatt tgcgtgccaa gcatggccgg gtgatgatcg ggatcacact
    16141 cgtggaagac ctggcggtgg tcgtgctcac ggtcctgctt ccggcgctcg gggctctgga
    16201 accgggccgc tttgccacga ttggaaaggc cttgtggctc gcggtcctca ttctggttcc
    16261 cttcgcgtat ctcgcggcga aagccattcc gccgttgttg acacgcattg cgaagacaca
    16321 aaatgccgaa ctctttctct tggtcgcgtt gtccctggga atcggcacgg cagccgtcac
    16381 acagatggcg gggttgtccc tggcgctcgg cgcgtttctg gcaggattga tcatcagcgg
    16441 ctcagaatat ggtcacgaaa ccttagcccg gctcctgtcg ctccgtgacg cttttgtggc
    16501 cctgtttttt gtgacgatcg gggccctcat cgatccgcgc gtggtgtggg ccaatctccc
    16561 gttgctcggc ctcatgctcg gattgattat cgtggggaaa ttcgtcattt ggacgctggt
    16621 cgtgaagctg tttcgttatg cctggggcac ggccctgctc gtgggcgccg gtctcacgca
    16681 gatcggcgag ttttctttcg tgttggttca ggtcgcgaaa acggcgggcc atgtgagcga
    16741 ggatgtgtac aatgccaccc tggccgcttc gctgctgagt attctcctca acgcgcccct
    16801 cgttcggtat gcgcctgggt ggatcgctgc attacatttt aaaatgggac gagtgtcctt
    16861 gcccattcaa ggcgggattt ctgacgaaca agccggacat gtggtgttgt gtggtttcgg
    16921 tcgcatgggc agcacggtgg gactcgccct cgatcatttt gagctcccgt acaccgtgat
    16981 tgagcgcgac ccggacatcg ttcgacagtt gcgccgacgc ggaatttcct gcgtctacgg
    17041 ggatgcgtcg caaactgaac tattgaaagc ggccggtgcc caacatgcgg cgctggtgat
    17101 cgtggcgctt cccgtcattc acgagacgtc gcttaccgtt cgacgattcc gggggctgaa
    17161 cgaaaaaatc ccccttctcg cccgagcgca tgggtttcgg gaggctgagg acctgaagga
    17221 cgtaggagcg acggaagtta tcctgcctga ggtcgagggg gcacatacct tgatccgaca
    17281 cgccttccag gccttgaagg tctcgaagcc gagcatcctc gattatctta agtcctgcca
    17341 agcgttccga tcgtccggtg agggacctga ttcagggaac gtggaggccg tgaaggccag
    17401 cgttgggcga agcacgtgaa tgtctgaatt aactgcggcg tcatccaatc tcttactgcg
    17461 atgccgcggt tcgaacccgc tcggcgcctc caaacagagc ttgctcgacc tggagccttt
    17521 acgattgttc ttggtggcgg gataacctag tcatcacctt ctcgttcatt ctgtttctgc
    17581 ctgccggcag cccgtgctga taccggcgtt cagcggctca cctcggcaat tcgccgttct
    17641 tgttctttta gtttccgact tgcggcaaga gtgtccggat ccagcaggcc atggagaatc
    17701 tcctgtctca gttgcatgaa cagcggctcg tgcctgacgg atgccggctg accgccttgc
    17761 agcagggtga gccagcgagt gctggaggcg gcatagcggc cgtttggcac ggtggtgcgt
    17821 aattcttgga aggcgtggat cgcatcctcg cggttttcga atagcgcgac caggcccctg
    17881 aggtacgtca cctcttcaca gctggcgttc tgttggcaat tcgcccgccg cgcttcctgg
    17941 gttcggctca ccacctgcaa tgcctctcgc tcttttacgt cggtctcaaa aaaggcgctg
    18001 ctcggcagcg tgacaaacgg ccccgcgccg gcacaaccaa aaaaaccgct gatgagcagt
    18061 acccagagtg aactgtgcgc aacgtttctt ccattcatac gatacctcca ggtagtaatc
    18121 ggatcgacag cctgtgtacg tgtcattggt tctgccgggt gatcgccggc tcgcctctcc
    18181 gcttagcccg gcgagtccca acattcgatg tgaaggactt gcaggctgag tcccgcttgg
    18241 cgcggatgtc tggctcagat tgcccggtag tctctaagga aagactcgat gcaacggtgg
    18301 ctgaattcga ggggatgaca acaggacagt tgcggcacct gagtagcccg ggattttgtg
    18361 tggacgcgtc agcacgggga gccgcctttc gagcgttgtc ggcggtggca ggccgcaggc
    18421 agtgggggca gagtgtgggg gatagccagc tgtgacctta cgccttctag ccggcaatag
    18481 caaaggcctg gagcgcggtg aatcggtcga tgctctggca ggcaccagac caccatggct
    18541 caactaggtg gttgtgtcag atggttgggg aacctgtttg gggctattgt gagcggagtc
    18601 ctgggaaggg caggttcccc tatcgtccga aatggtaggc gatgccgaac atgaagaggt
    18661 tggcgcggta gtctgccgtg atgtcgctac ggttgaaggt gaactgagcg aagttgtact
    18721 tgtactcgac gaagggggcg atcgaatcgg tcatatagta gcggataccg gccaacacgt
    18781 tgaagcttgg ggcaaaggag gcttccgggc ggaaccctcc gctggagata ttgccaacat
    18841 tgagtccgat accgagacca gcatagggtt ccaccttgta ctgatagccg gtcacccgaa
    18901 ctaatgcatt aaaagccagt gagtgcacca tcaaatcggc accagggatc gtccctgaca
    18961 gtcgagggtt gctggacgtc ggtaaggtct gttgtttgat gccgggtgtg gaacggtacg
    19021 cgtccacttc aagcccgaac caattgtagc cgcgatcgtt aaaataatgg ccgatcttgc
    19081 ctccgtaaaa caggtctttt tggagggaca cttttccggc atcggtggtg tcaaaggtgg
    19141 gggcgttcat accgacgcct gccgcaacat acgtttctgc aggggtgcgc gcaggaaagc
    19201 ccagcatgac caggaataga aatgcgacta accagcgacc cattattcct cctctcacag
    19261 acaaggatta acccaaaaga ggctcagggt gctactctaa cgggcttcac gggaggtggc
    19321 aagcgagaag tgtatccgag tctgcctcat aaaccgtcaa tggccgattg tgaacccgag
    19381 gccccctcag ccgtcacgtg tcagtcttca ttggtccgga atacatctga gaactcgacc
    19441 atggttgacg cacgtcagca ctgcgtctcc tctcggtgca acaacgtcgg ccgtaggcag
    19501 aatcctcgcc atctcgattg atcccaggac cagttgagca gggcatattc ggtgtgggtg
    19561 atacgctgtt cgatggttcg tccgaacccg ccgatgcgga gagaaaaacc tgcgggtaag
    19621 gccatgtctt tcacgaggcc gaattcagta aaatcacccg catagtaggc tgggtcgccg
    19681 cgttcggtca cgaagtgttc tccccgccac agagtgacat aggggcgcca cccatcgaga
    19741 tttaacccgg ctgtcaattg atacccccgg ccacggacaa tcggggaacc gccttgcgtc
    19801 ggctcattct ggctggtgag gtagagggcc atcacatcga tttcacggaa ccaggacagc
    19861 tgcgaccagt actgtctggg ttcaaaagtg aattgaggac cgaaggccgt catgaagttg
    19921 ttgccggcgg ggcgatcacg aggaatgcct ggcccgaaga aggtccttga ttcagagtat
    19981 tgcgcaccgc cggagtgaga ccaatagacc tggccattga gggagaaatg accggccgaa
    20041 acccgacccg cgtagcccac atcaaagcgt tccgctttct gaaaagtttc cacctggctc
    20101 caattgataa agagatcctg ttggtagtgt tctcgggcca ctaggagttg gaacccctgt
    20161 tcgatcggac ggacaaataa catcgcgtcg ttgaagagcg cgtctacaaa gggatggttg
    20221 cgatggagcg tgcctgccac cacacgcata tcgggaacca tttcgtattc ggcagagaga
    20281 atgggctgca cggcatgaaa ccgttgagtg tctccaaccg gcaggccggt gaaaaagccg
    20341 gcattgagtg tcagctgtgg gctgactcga tattgaagca ccggtgaaag atattggccg
    20401 gcaaacgttt gatttgagac gggaacccca ccaatctgtc gtacgccggg ataggcgata
    20461 ttataaacat aggtcagcgc gtcgagtttg gcgctgaacc ggcgttccga tgacgcatcc
    20521 tggcgatcgc cgaagggcct ggacatcgag gcccacaggt caccgatcag atctgcgtgg
    20581 ttggtgtccc aattccaatt gaagaccaga tattcctggt tcacccactt aaaggtgtcg
    20641 cggttctggt acgtgatgcc gtcttcggtg aaaaaggccc ggatgcgccg tgcttgcgct
    20701 ccgaattcga tggaggtgcg gtctcccagg cgaactgtct tcgagaggcc gatttccggg
    20761 aaattgctcg cccggaactc cggatcgccc tgctggctga aaaagttgcg gcctttccag
    20821 aaggcgaggt aagggcgcca tcctgccaga tcgagccagg cgtggagttc gtagcctcgt
    20881 ccacgggtag cctcagcgcc ggcatccgtt tgattgtaac ttgtgaggta ggtgaaccgg
    20941 attccgagtt gattccacca ggggagagcc gggaaatagc ggaagggttc cagggccaac
    21001 tcagggccgg cggcgaccac gaggttgtcg ccggcttgga acgaccggtc gagtttgaag
    21061 agggcctgcc cgttgcgcac ccaatgggcc tggccgttga agcgcaaggg gccgaacttt
    21121 agctggccgg catagccgac atcgtaccgg ttgggcgccg atccgcggaa cgcctgttcc
    21181 cagttgatga acaggtcttg ccgatagtgt gtggaatcca ctagcatttg agccccttgc
    21241 tcaatcggac gcaggaagcg attgccccgg tagaacacgg cattgtggaa atcctggtgg
    21301 ggcacgtaca gggtcccaat cactccggtg acggcatcgt tcggctgata ggtcagccgc
    21361 atgatggggc ggacttggga gacttcgtgg tcatgcccga atggcatgtt cgcgaatacg
    21421 ccgagttcag cttccagcgt ggggagtagg cgtttgtaga tcgaggcgtt caccagattg
    21481 ccgatcaggg tcgtgtcccg tcccaatccg ttggtaaagg tattgtcttc caggccctgc
    21541 ttctccagat tgagcaggaa agtcgtcgtg gagaagcgca tatcgatcac gtgaggggtt
    21601 gccgggggtg ccgtgtcctc ggcgaatgtc gcgagaggga tagtcaatag tgcggcgata
    21661 aataggaaaa ggcttgggct gcgagagtgt tgttgaggca agtggtaggg cttgggtttc
    21721 acacgtgggg tcagatgata tgaagagagc acgcgccagg tcaatatgtg agactggagc
    21781 aattatcatg tgaggagcag atcctgagaa gtaggtcagc cgatgccgtg ggggagcaaa
    21841 tcgttgagga agaatctttc gtgggccttg aatggagcca ttttctatgt tttttctcag
    21901 tgggtgctcc tcatcattct tgccaagctg ggcggcccca cgatagtcgg tcggtatacg
    21961 ctaggtctgg ccatgacagc acccatcgtt cttctttcta acctccagct tcggacactc
    22021 ttggcgagcg atgtgcaaga aatgtttccc ttctcagcct atgccatctt gcgattgctt
    22081 acgagcctag gagcgctcct tctcatctgt gggctcgctg cctgcctgga attcacactc
    22141 gaccaagctg ttgtcatatg cctgattggc ctcgcgaagg tcattgagag cggatcggac
    22201 attacctacg ggctaatgca aaagcacgaa agaatggacc tggtgagtct ttctaaaggc
    22261 atacgaggta ttttgacggc gggcgcagtt ggcgggattt tttgggggac caattcactc
    22321 attgcgggcg ttggggggat gatcgcggta tggctcatgg tgttcgtact gttcgattgt
    22381 cgtcacctgc aaaggatcct tggaaatcca ccaagtcaca tggcatgggc aaagatggtc
    22441 catgccaacc tagcatcaaa gattttgagt ttgagcaaat tctccctctc ccttgggatg
    22501 gtttccatgt tgatgtccta taacaccgct atgcctcgat acctcttgga ggcatatcac
    22561 ggagaggctg cgctcgggta cttctcagct attgcgtgct tcactgtcgc ctgcacgata
    22621 gtaccggaag cctttagcca ggcggctctt cccaggttgg cgaagtatca tgccaactca
    22681 ttggatagct attggcggtt gatgggtaag ttgatgctcg tggcattctt aatagg
//
LOCUS       DAORQN010000014        22502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_163_length_22502_cov_27.683120, whole genome shotgun sequence.
ACCESSION   DAORQN010000014 DAORQN010000000
VERSION     DAORQN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22502
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163_length_22502_cov_27.683120"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1854
                     /locus_tag="PK614_02100"
     CDS             172..1854
                     /locus_tag="PK614_02100"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRB81034.1"
                     /translation="MTDLEIARSVPHKHIFEVAQSLGIHSDDLIPFGRYKAKIAPTLG
                     ERIQNRPLGRYILVTAINPTPLGEGKTTTSIGLSMGLCRLGHRAAVTLRQPSLGPVFG
                     IKGGGTGGGRAQVHPMEDINLHFTGDAHAVSASHNLLSAFVDNHLFHGNTLKVDPKGI
                     RWPRTLGVSDRALRDILLGEETGRRPGKFVITEASEVMAVLALATDHADLRQRLGRIL
                     IGLTEGGQMVRAEEFGCAGSMAVLLKDALLPNLVQTLEGTPAFVHAGPFGNIAHGNCS
                     IVSDRLALRCADYVVTEAGFGSDLGAEKFFNIKCRTSGLRPDVGVVVATLRALKLHGG
                     GGIVKSGAPLPPGVTGPNQAALEQGFANLEQHIANVRAHGVPVVVAVNAFKDDSTDEL
                     NWVCERALEGGASAAAVSTHWSDGGRGAEELARAVVKVAAQGTQFSPLYEVTSPIKKK
                     IETIATTIYGAAGVSFSPQAERDVELATRLGFDRLPICMAKTPLSLSHDPAVKGRPSG
                     FTVPIQELRILAGAGFLTAVCSGIQLMPGLPKKPAGERIDVDPQSGQIVGLQ"
     gene            complement(1862..2722)
                     /gene="hflC"
                     /locus_tag="PK614_02105"
     CDS             complement(1862..2722)
                     /gene="hflC"
                     /locus_tag="PK614_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRB81035.1"
                     /translation="MSKQGFILAFVGIVLGLLVLGASPFYIVDVTQNAIVVQLGKPVR
                     NVTEGGLYLKMPFIEEVTYFDKRLLDYDSNAQDVITQDKKTLLLDNFAKWRITDPLKV
                     YQAFQSQRGALQRLHDIIYSELRVELGRHDLAEIVSSARAQLMAVVTQRANEKASAYG
                     IEIQDVRIKRADLPEQNEKAVFSRMQAERERQAKQYRAEGAEEAQKIKSEAEKDREII
                     LAEAYRESEELRGGGDAKAFRIYADAYRQDPHFFEFTRTMEAYRKTLKDKTTILVSPE
                     SEFFRYLKQR"
     gene            complement(2719..3756)
                     /gene="hflK"
                     /locus_tag="PK614_02110"
     CDS             complement(2719..3756)
                     /gene="hflK"
                     /locus_tag="PK614_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRB81036.1"
                     /translation="MVWDPKDPWSKKGDDLDQAFKQAQGQLRNLLPTGGFRNLLLVAF
                     TVFLIWQSAFIVAPDEEGVVKRFGIPVRVVDPGPHMKIPIIESVLQPKVAKLHRVEIG
                     FRKDRQGRQQMVPQEALMLTGDMNILAIEFIVQYKIKSSREYLFNVADIDETIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQNDTQELLQHILDDYRTGVQVAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQAQGYRNDITPKAKGEAAQLVNQAKGYAQARLNRSQGES
                     NRFLATLKEYNQAKDIISKRIYIETLEDVLPHIDKFVLDGKGADRALPYLPLDRFSKP
                     APSSSTQERTP"
     gene            3898..4203
                     /locus_tag="PK614_02115"
     CDS             3898..4203
                     /locus_tag="PK614_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRB81037.1"
                     /translation="MRNPARAAELFHALADPTRLEILDELKEGECCVCELTDRLQAGQ
                     SRLSFHLKVLKDAGMILDRREGRWMYYSVNADALAELDDLVDSLKQAKRARRATGCC"
     gene            4261..5085
                     /locus_tag="PK614_02120"
     CDS             4261..5085
                     /locus_tag="PK614_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRB81038.1"
                     /translation="MDAQAIRELVKEKYGEAAARAKSGGSSCCGASPALMNVDPITSN
                     LYSDQERQGLPADAVAASLGCGNPTALAELHAGETVLDLGSGGGIDVLLSAKRVGPTG
                     KAYGLDMTEQMLALARENQRAAGVANVEFLKGEIEHIPLPDRSVDVIISNCVVNLSGE
                     KERVLAEAFRVLRPGGRLALSDIVVRGAVPSEIRRNLELWAGCVAGALEETEYRDMLA
                     QAGFVEIGIEPTRIYQADDVKELLVGTDLSSDLLVAQVEGKFMSAFIRAKKPAVTV"
     gene            5123..6217
                     /gene="arsB"
                     /locus_tag="PK614_02125"
     CDS             5123..6217
                     /gene="arsB"
                     /locus_tag="PK614_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRB81039.1"
                     /translation="MELALSVGEPQVATKRLSFFERYLTLWVALCMVAGVLIGQAVPG
                     LVSSLRGAEIGQGSHINLPIAVLIWLMIVPMMMKVEFASVRDVGKRPVGLLITLFVNW
                     VVKPFSMAFFAWVFFRYVFSAWISPAEADQYIAGTIILAAAPCTAMVFVWSYLTDGDP
                     AYTLVQVAVNDLIMLVLFVPIVGLLVSGASSLAVPFDVLLYSVAIFIVIPLVIGAGLR
                     LWLVRRYGLRWMEELFLPRFAPVTIAALLLTLVCIFAFQSQNILGKTVHVILIAVPIL
                     LQVYMNSSLAYGLMHRWRVPYAIAAPGALIGASNFFELAVATAIALFGPESGAALATV
                     VGVLVEVPVMLSVCSVCNKTRHWFNQEVGT"
     gene            6214..6654
                     /locus_tag="PK614_02130"
     CDS             6214..6654
                     /locus_tag="PK614_02130"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRB81040.1"
                     /translation="MKARVLFLCTGNSARSQMAEGWLRHLAGDRFEVFSAGTHPVGLN
                     PGSVEAMAEVGIDIAGHRSKHVSEFLTQPFDYVITVCDRAKESCPTWPGPTHLLHWSF
                     DDPAAVTDCDEAHRQLFRRVRDEILGQIKGFLAQEEKSLVSGPC"
     gene            complement(6651..7688)
                     /gene="arsS"
                     /locus_tag="PK614_02135"
     CDS             complement(6651..7688)
                     /gene="arsS"
                     /locus_tag="PK614_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HRB81041.1"
                     /translation="MGLSLLARRDPLAAASEQLRLLAQTTACQPFETALNAVELYPLH
                     ATGITTLQLNLGKLCNQTCRHCHVDAGPDRTEVMSKETLDLCLQALARTDIPTVDITG
                     GAPELNPHFRWAVEQARLLGRQVLDRCNLSVLLLPSQADLGEFLAQHRVEVIASLPSY
                     QATQTDAQRGEGIFDKSMESLRLLNRLGYGKEGSGLTLNLVYNPVGAFLPPKQEGIEA
                     KFRKELASRHGVNFTRLYTITNMPISRFLEFLIESGNYEGYMERLAAAFNPAAAAGVM
                     CRYTLSVGWDGTLYDCDFNQMLELPVSQGTPQHIRDFDPARLHHRQIVTRNHCYGCTA
                     GSGSSCGGAVT"
     gene            complement(7719..8906)
                     /locus_tag="PK614_02140"
     CDS             complement(7719..8906)
                     /locus_tag="PK614_02140"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB81042.1"
                     /translation="MAIDDITQKVSDRYARAAATGEQMCCPTGYDFADLKSFVPEEVL
                     TISYGCGTPAGLNTVQSGETVLDIGSGGGIDCFEAARRVGPTGRVIGIDMTDTMLEIA
                     RRNAPIVAGNLGYATSNIEFRKGMADAMPVEDASVDLIISNCVINLAPDKRTVFREMY
                     RVIKPGGRFTISDIVSDQVVPQYLVHDAAKWGDCLSGALQVQDYIGGMVDAGFRAVHQ
                     IKSTPWQSIDGIHFLSVTLTGYKFPAIADEKTSSYATLRGPFSSVTDELGQRYERGVP
                     QSIDAQRAQLLQSEPLRSLFLLGPTPVTLAATDPRWCAIHPEQKPCVWQGAYAILTGP
                     IISAEDDDHHQYYRGVPLEICSKTLQVLTQEAYRPHFTIYQRASAGVDGTEVACSPTG
                     GCC"
     gene            complement(9042..9518)
                     /locus_tag="PK614_02145"
     CDS             complement(9042..9518)
                     /locus_tag="PK614_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HRB81043.1"
                     /translation="MSQRMASLSLILGLALTVAACNTVGSGPAGTAAAEKSLYDRLGG
                     KTAITAVVDDFVGRVAADTRINGKFANANIARLKSMLVDQICQASGGPCTYTGRDMKS
                     THAGMGVSSSDFDALVGDLVATLNKFKVPEREKNELLGALGPMKSDIVEKPMASMQ"
     gene            9741..10346
                     /locus_tag="PK614_02150"
     CDS             9741..10346
                     /locus_tag="PK614_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB81044.1"
                     /translation="MIQSQAVAQAPSIHDQEWTGLLARIAAGDQPALAEFYDASSAKV
                     FGLAMRILADRTAAEEVTMDVYTQVWRRASTYDTERGTPGSWLMMLAKTRAIDRFRSS
                     YLERGRQVPLDQAAELPGDAATPEQYSADLERQRLVQEAMASLSAEQRQAIALAYYWG
                     LSQSEIAGRLRLPLGTVKTRMRLGMIRLREVLAPHGEGVRS"
     gene            10343..10927
                     /locus_tag="PK614_02155"
     CDS             10343..10927
                     /locus_tag="PK614_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB81045.1"
                     /translation="MTDPRHKEDMSELAALYALEALGDDEQRQFKQALETAPPEVRQQ
                     VAAFQEVAQELAFSGPAIAPPASLKAKLMARIAQEPQEREGARDFTCVRSGQVAWRDL
                     GPGVSMKLLFNDSAGARATMLLRLAPGGKLLAHRHRQVEEFYVLEGNCICAGEFLQAG
                     DYHRAEAGSVHPITSSEQGCLVLVMTSTQNEPAG"
     gene            complement(10933..11310)
                     /locus_tag="PK614_02160"
     CDS             complement(10933..11310)
                     /locus_tag="PK614_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HRB81046.1"
                     /translation="MWYRIGADLVLLLHLAFVLFVVTGGLLLLKWPRLAWVHLPAAIW
                     GAIVEYTGWICPLTPIEHTLRAMAGESAYGSDFIGHYLLPLMYPAGLTSIIQILLGTA
                     VVLVNVTIYWRVFGRSRKEQQQE"
     gene            complement(11382..11789)
                     /locus_tag="PK614_02165"
     CDS             complement(11382..11789)
                     /locus_tag="PK614_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRB81047.1"
                     /translation="MSSGPPNQSRRMRHDSFKDFVLDQLGEMPELTWKAMFGGYGLYQ
                     RTTFFGIIHKGRLYFKTNDVTQALYRARGMHPFRPNAKHTLAQYYEVPTDILEDPDHL
                     IVWARSAVTLPTAQLPLPFTTAPPQGYDLPAQM"
     gene            complement(12104..12577)
                     /locus_tag="PK614_02170"
     CDS             complement(12104..12577)
                     /locus_tag="PK614_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRB81048.1"
                     /translation="MSTLIRWDPFRVQWNPLKERDELENRLSTLLGHRASTGNGGKEA
                     LTVAEWSPLVDIVEDENEYRIKAELPAMKKEDVRLTVDNGVLTISGERKYEQEEKREK
                     HHRIERAYGSFVRSFSLPEDADGSKVTADYKDGVLHMHLPKSEKAKPKSIEIKVS"
     gene            complement(12634..12855)
                     /locus_tag="PK614_02175"
     CDS             complement(12634..12855)
                     /locus_tag="PK614_02175"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB81049.1"
                     /translation="METNVSRTTPQRAGTAHTSPSHDKAAHAGQAVKGTAAIALRAYE
                     LYEKRGRKDGQALEDWLNAERQLAAAAGH"
     gene            complement(12982..13539)
                     /locus_tag="PK614_02180"
     CDS             complement(12982..13539)
                     /locus_tag="PK614_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRB81050.1"
                     /translation="MTTRNVLTLCAALVLGGAIITGSAHAIGKADEKTPINDSWITAK
                     TKIALFADSRVKGSEINVETAQGAVMIRGKVDSDAAKQAAEGIAKGIDGVKTVKNDLQ
                     VVAPSKREATDDKDDAITARVHEHMEKDAHLKKAGIHAQTNAGVVSLSGEVQDLATSA
                     EASWSAWQVPGVKAVKNDLTVKEKA"
     gene            complement(13602..13829)
                     /locus_tag="PK614_02185"
     CDS             complement(13602..13829)
                     /locus_tag="PK614_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRB81051.1"
                     /translation="MNADQLKGKWLQFKGDLKQQWGKFTEDDLQQIAGNYDTFVGKVQ
                     ERYGEKKAELMKWASHWHEQAKPDAEGTKAP"
     gene            complement(13924..14091)
                     /locus_tag="PK614_02190"
     CDS             complement(13924..14091)
                     /locus_tag="PK614_02190"
                     /inference="COORDINATES: protein motif:HMM:NF018715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRB81052.1"
                     /translation="MLYYALMFLVVGLIANALNLLGVSAVAVQMSWILFLIGVVLLVV
                     HLVSGRTPRTT"
     gene            14508..14786
                     /locus_tag="PK614_02195"
     CDS             14508..14786
                     /locus_tag="PK614_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81053.1"
                     /translation="MRTFIIAAIAGFCLLTGLSFSAGAGETKAKIEKGKGELKGDLEE
                     MKGETKAMKEELKGNDTKAEMERAKGKLKGTGERAKGKMKELKEKATD"
     gene            complement(14862..15719)
                     /locus_tag="PK614_02200"
     CDS             complement(14862..15719)
                     /locus_tag="PK614_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB81054.1"
                     /translation="MPSQTPEAVIEAQRQDWNRVAAGWDKWDQFFNRNMAFINHRLVA
                     DARVRPGLRVLDLGSGTGYPALLAGEVVGSDGTVVGIDLAESMLAVATRKAKTLGLEH
                     VTFRTGDVTSLPFETGSIDAVISRFCLMFLPEIPKAAAEIARVLKPGGYVAAAVWSAP
                     DKNPFIRIPMDVIKTITPLPPPDPDAPGIFRLAKPGDLAGMFERAGLKPLDDEEFTAE
                     VTYATAEEFFRGLMDIAAPIQNLFAKLTKEQQAEAERGIIKAVNEYRGTQGVSLPIAV
                     RIVSARKPQ"
     gene            complement(15757..16026)
                     /locus_tag="PK614_02205"
     CDS             complement(15757..16026)
                     /locus_tag="PK614_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81055.1"
                     /translation="MYYSDGDQLMLTHYCSLNNQPRMRAAAIKEGDKAITFAFVDATN
                     LTNPTDVHMRQLTIDVKDHDHFTQTWTLSKAGKDVPKVFTFERVK"
     gene            complement(16016..16261)
                     /locus_tag="PK614_02210"
     CDS             complement(16016..16261)
                     /locus_tag="PK614_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81056.1"
                     /translation="MPRGILRTLCGFVLLLVPLFAQAADTPDTTLSPLKFLVGDWKGA
                     DAEGKAHKIAFTLSSGGTTLTEILTPPDSPPDDDHVL"
     gene            16835..17431
                     /locus_tag="PK614_02215"
     CDS             16835..17431
                     /locus_tag="PK614_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HRB81057.1"
                     /translation="MAHHHSATITVPRHAEPRDHDHHTNGHDCVQAHGDHLGRHQPHE
                     HQEANHQAPSLDRTALMATLHCLSGCTIGEVLGMAIGTALGWSNWPTVGLAVALAFLF
                     GYAMTLYPLRRAGMAWGTALGLAFASDTLSMTTMELVDNAIMVMIPGAMDAGLPDPLF
                     WGSLVLSLLLAGVAAFPVNRWLIARGKGHALVHGQHCH"
     gene            complement(17611..17775)
                     /locus_tag="PK614_02220"
     CDS             complement(17611..17775)
                     /locus_tag="PK614_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81058.1"
                     /translation="MNTCSVVRFSRPAGLFSFGLLDGLQVRLRQPRLREAPAGFGLER
                     LATTFMNNPG"
     gene            17826..18773
                     /locus_tag="PK614_02225"
     CDS             17826..18773
                     /locus_tag="PK614_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81059.1"
                     /translation="MTIGKYMRMLAIVATAMVAFALAGCSQFEPRDKRFYYRALWNFS
                     LREDLAELDSEFNGVDFGHSNLYENLLLTGGQDVPAIEERARKQTLAFIATKPRLNPN
                     EEAIAPTYMKLAWRAQNTFDEAHALHRATYDIAVSDEPEKDRAIRNVLAYYKDSAYAI
                     TSKRLDHHRLDQFPYSKAFRTRFPLFNATIWSYHYLQVAVYDPLQAARDLAAKTQAVR
                     PILATYRRYLEQPPVQWTFMPLTAELSPQFAARYPELANIFDNLHMLHDNISDILTSE
                     RLPTWEAKRVEIYRLLNHYYLASADATNPMIVQGQEHHH"
     gene            18770..19555
                     /locus_tag="PK614_02230"
     CDS             18770..19555
                     /locus_tag="PK614_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRB81060.1"
                     /translation="MKLRGKRFLARLLGAALALVTTPLFALDHDNLDPNRPIGMEDAY
                     AIPKGEIGLEGGVRFNDRREGRTQVTFQPQIIYGAFANTQIEIQGDLFTDPRSIVGAN
                     KSGDLHLGVLYNFNTETLMLPALAVRVEADLPTGVNSKGVDTQLTGILTRSFGRLRVH
                     LNAGYTVMGSPQGQERPGAYRAVAAVSYPLGYPTSFRDTLIASVYTRQSDLRGQQNHT
                     GIEVGIRHQLTSRVVLDGGLGTEFVGPADRAALLGAMGVSVGF"
     gene            complement(19610..20320)
                     /locus_tag="PK614_02235"
     CDS             complement(19610..20320)
                     /locus_tag="PK614_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRB81061.1"
                     /translation="MNGFDTTVLGFFNQFAQRSWAFDSLIMTISDSDALKGYLSLPLI
                     WWAWFRAPHREDHRQTVLLTILAAFAAMAVARALQLVLPYRARPVHTAGLDFTLPYTM
                     PADFLAGWSSFPSDHASLFFALAAGLWLVSPFVGALAFLHAGVIVCLPRIYVGLHFPT
                     DILSGACLGVTAMAVIWKWRSVFVKLVEPALAWSKSIPHLFYPAFFILTSELAHLFKD
                     SRWLAESLWHMAKLSLAR"
     gene            20653..21546
                     /locus_tag="PK614_02240"
     CDS             20653..21546
                     /locus_tag="PK614_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81062.1"
                     /translation="MGRAGRIARGIMVLVSVLLVAACGDSFNDAYCEANNHRNQQTVE
                     HLTNVLDQKRNQPGLYLARARCHYFLGAYAQALQDASEVIRRLPNKADAYLVRAKAGK
                     MLGQIPQALQDYSAAIKFQPTAEAHYGRGLTYVREYQGKDREALEDFTAAIRLDPKHV
                     GAHAFRAQIYSRHEQFQHALADSETVLKLDPTTPNAYCNVGFAHYALGHDAEGRRFLT
                     TCYQKDPDPQTRGYYETEVNKVLYARKPKRNSGGSYVAEGGVKTPYDREMERQQNVYE
                     SLRNSGFNDRAEACRTDGSKC"
     gene            22322..>22502
                     /locus_tag="PK614_02245"
     CDS             22322..>22502
                     /locus_tag="PK614_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81063.1"
                     /translation="MAWGPDANTEDELKKLAAARGYFQQEFPGASIRDSYDAGKMAQV
                     FRIEADDVGGLCDAVV"
BASE COUNT         4379 a         6302 c         7158 g         4663 t
ORIGIN      
        1 aagaagtttg cctgttccct tcctctttct ccgttgtaat cctcgactgc ccgcgtccca
       61 gtccagctct accagccctg tgctacccag tctttgcggc ccattacttc ggcgaatgcc
      121 gtggtatact ccgcactttc ctgaaattcc tgctctcacg aggaacattg catgactgat
      181 ctggagatag cccgttccgt ccctcacaaa catatatttg aagtcgcaca gtccttaggc
      241 attcattctg atgatctcat cccctttggg cgctacaagg ccaagatcgc gcccacgctg
      301 ggtgagcgaa tccagaacag accgctggga cgatacatcc tggttacggc catcaatcca
      361 acaccccttg gcgagggcaa gactacgacc tcaattgggc tgagcatggg gttgtgccgc
      421 ttaggccatc gggccgccgt cacgctcagg cagccttcct tgggaccggt cttcgggatc
      481 aaggggggcg gtaccggagg cgggagagcc caggtccacc cgatggaaga tatcaatctg
      541 cattttaccg gcgatgcgca tgccgtttct gcgagccaca atcttctttc cgcatttgtc
      601 gataaccatc tgttccacgg aaatacgctc aaggtcgatc cgaaagggat tagatggcca
      661 cgcacgcttg gggtgagtga ccgcgcgctt cgcgacattc tcctgggaga ggaaaccggg
      721 cggcggccgg ggaagtttgt gattacggag gcgtcggaag tcatggcggt gctggccctg
      781 gcgaccgacc atgcagatct tcgccaacgc ctcgggagaa ttctgatcgg cctgactgag
      841 ggcggacaga tggtccgtgc tgaggaattt ggctgcgccg gctccatggc cgtcttgctg
      901 aaggatgcct tgttgccgaa tctcgtgcag accctggagg gaactccggc atttgttcat
      961 gccgggccgt tcggtaacat cgcgcatggg aattgctcga ttgtctccga tcggttggcg
     1021 ttacgttgtg cagactatgt cgtgactgaa gctggattcg ggagtgattt aggcgcggaa
     1081 aagttcttca atattaagtg ccgcacgtcc ggtcttcgtc cggatgtcgg ggtggtggtg
     1141 gcgaccttgc gtgcgctcaa gcttcatggc ggtgggggga tcgtgaaatc cggtgccccg
     1201 ctgcctcccg gtgtgaccgg gccgaatcag gcggcgctgg agcagggctt tgccaacctc
     1261 gaacagcata tcgccaatgt ccgggcccat ggagtgccgg tggtggtcgc ggtgaacgcg
     1321 ttcaaggatg attcaaccga tgaactaaac tgggtgtgcg aacgcgccct ggaagggggt
     1381 gcgtcggcag ctgcggtgtc gacccattgg tccgacggtg gacggggagc cgaggagctg
     1441 gccagggcgg tcgtcaaggt cgctgcgcaa ggtacgcaat tcagtcctct ctatgaggtg
     1501 acctcgccca ttaagaaaaa gattgagacc atcgccacta cgatctatgg agcggccggc
     1561 gtgtcattct cgcctcaggc cgagcgggac gtagagctgg cgacacgatt gggattcgat
     1621 cgactgccca tttgtatggc gaagaccccg ctttcactat cgcacgatcc ggcggtgaag
     1681 gggcggccct ccgggttcac ggtgccgatt caggagttgc ggattctggc cggggccggg
     1741 tttctcaccg cggtctgctc aggcattcag ttgatgccgg gtctgccgaa gaagcctgcc
     1801 ggggaacgga tcgatgttga tccacaatcg ggtcaaattg ttggcctgca gtagtggagt
     1861 gttaccgctg tttcaggtac cggaagaatt ctgactcggg actgacgagg atggtggtct
     1921 tgtccttgag ggtcttgcga taggcttcca tggtgcgggt aaactcgaag aaatgcggat
     1981 cctgacgata ggcatcggca tagatgcgga acgctttggc gtctccgcct ccacgcaatt
     2041 cctctgattc tcgataggcc tctgcgagga tgatctcccg atctttctcc gcctccgact
     2101 tgattttctg agcctcctcc gcgccttctg cgcgatactg cttcgcttgt cgctcccgct
     2161 cggcttgcat gcgcgagaac acggcctttt cattctgctc aggcaggtcg gctcgtttaa
     2221 tccgcacgtc ctggatttcg atgccatagg cagacgcttt ttcgttggcg cgttgggtga
     2281 ccacggccat gagttgcgca cgggccgatg agacgatttc cgccagatcg tgccggccta
     2341 attccacccg cagttccgaa taaataatat cgtgcaggcg ctggagcgcg ccgcgctggc
     2401 tttggaacgc ctgatacacc ttcagcgggt cggtgatccg ccacttcgca aaattatcga
     2461 gcagcagggt cttcttgtcc tgggtaatga cgtcctgcgc attggagtcg tagtccagaa
     2521 ggcgcttgtc gaagtaggtg acttcttcga tgaacggcat tttgaggtag agcccccctt
     2581 cggtgacgtt ccggaccggt ttcccgagct ggaccacaat ggcattctgg gtgacatcta
     2641 cgatgtaaaa aggcgatgca ccgaggacga gcagcccgag aacaatgccg acaaatgcca
     2701 ggataaatcc ttgcttgctc atggcgtgcg ctcctgtgtc gaactggacg gggccggttt
     2761 ggagaaccga tcgagcggca gatacggcag cgcacggtcc gcccctttgc cgtccaagac
     2821 aaatttatcg atgtggggga gcacgtcttc aagggtctcg atatagatcc gcttgctgat
     2881 aatgtctttc gcctggttgt attctttcag cgtcgcgagg aatcggttgg attcgccctg
     2941 ggagcggttg agccgcgcct gcgcataccc tttggcctgg ttcaccaact gagccgcttc
     3001 acccttggct ttgggggtaa tatcgttgcg atagccctga gcctggttga tcagcttttc
     3061 acggtcttcc ttggcgttgg tgacgtcctt gaacgcggcg gccacggctt cgggcgggtc
     3121 gacatcctgc aactggaccg cggcaacctg cacgccggtc ctatagtcgt cgaggatgtg
     3181 ttggagaagt tcctgcgtgt cgttctggat ttgcgctttg cccgttgtga gagcttcatc
     3241 gattttgctc ttgccgatca cttcccgcat agaggcttca gccgccttcc cgatggtctc
     3301 gtcgatatcg gccacattga agaggtactc gcgcgaactt ttgattttat attggacgat
     3361 aaattcaatt gccagaatgt tcatgtcgcc ggtcagcatc aaggcttctt gcggcaccat
     3421 ctgctggcgg ccttggcgat cctttcgaaa tccgatctcc acccggtgaa gcttcgcgac
     3481 cttcggttgc aagacgcttt cgatgatggg aatcttcatg tgtgggcccg gatccacaac
     3541 gcgcacgggg ataccgaagc gtttcaccac gccttcttcg tccggtgcca caataaaggc
     3601 gctttgccag atgaggaaga ccgtgaaggc gacaagaaga agattgcgga aaccgccggt
     3661 aggtaacaga ttgcgaagtt ggccctgggc ctgcttaaag gcctgatcca aatcgtcgcc
     3721 cttcttgctc caagggtctt tggggtccca gaccatgtag tggctcaatt cagtgagggt
     3781 ggcaatgtcg ccagcggatc accttagcaa agtgaggacc gtcgagcaac acataaaggc
     3841 ggccggttga cttatcaaga ttcgttgata tatatttttg tcatgaaggg aagcacggtg
     3901 cgaaatccag cgcgggcggc ggagttgttt cacgcgcttg cggacccgac gcgcttggag
     3961 attctggacg agctgaaaga gggcgagtgc tgtgtgtgcg aactgacgga tcgcctgcag
     4021 gccgggcagt cgcggctctc atttcatctg aaggtactta aggacgccgg aatgatcttg
     4081 gatcgacgag agggccgatg gatgtactac tccgtgaacg ccgacgccct tgccgagctc
     4141 gatgacctcg tcgattcgct caagcaggcg aagcgggcgc gccgcgccac cggctgctgt
     4201 tgattttttt tctgcctaat agatcaatga atcttgatgc ataaaagagg aggcgacgaa
     4261 atggacgcac aagcgattcg agagctggtc aaggaaaaat acggtgaagc ggccgctaga
     4321 gccaagagcg gaggcagttc ctgctgcggg gcctcccccg cgctgatgaa tgtcgatccg
     4381 atcacgtcca acctgtattc agatcaggag cgtcaggggt tgccggcgga tgccgtggcg
     4441 gcctcgctgg gctgcgggaa tccaacggct ctggcagaac ttcatgccgg tgagacggtg
     4501 ttggatctgg gatccggcgg cggcatcgat gtgttgctct ctgccaagcg agtcggtccg
     4561 acggggaaag cctatggctt ggatatgacg gagcagatgt tggcgttggc cagagaaaat
     4621 cagcgcgcag ccggcgtggc gaacgtcgag tttctgaagg gcgaaatcga gcacatcccg
     4681 ctgcctgatc gttccgtgga tgtgatcatc agcaattgcg tcgtgaattt gtccggcgaa
     4741 aaggagcgcg tgctggcgga agcctttcgc gtcctgcgtc cgggaggccg gctggcgttg
     4801 tccgacattg tagtgcgagg ggcggttccc tcagagatcc gacggaatct cgaattgtgg
     4861 gctggttgtg tcgcgggcgc gttggaagag acggagtatc gggacatgct ggcccaggcg
     4921 gggttcgtcg agatcggaat cgagccgacg cgcatctatc aggccgatga cgtgaaggaa
     4981 ttgctggtcg gcacagacct ctccagtgat ctgctcgtgg cccaagtcga gggcaagttc
     5041 atgagcgcat tcattcgggc caagaaaccg gccgtgacgg tctaactccg ccgggcagcg
     5101 ccagcacgaa ggagcaggag cgatggaact cgcattatcg gtcggtgagc cgcaggtggc
     5161 tacgaagcgg ctgtcgttct ttgagcggta tctcacgctc tgggtggcgc tctgcatggt
     5221 ggccggtgtg ctgatcgggc aggcggtgcc cggtttggtg tcgagcctgc gaggggctga
     5281 gatcggacag ggcagccaca tcaatctgcc gatcgccgtg ctgatctggc tgatgattgt
     5341 gccgatgatg atgaaggtcg agtttgcctc ggttcgtgac gtgggcaagc ggccggtcgg
     5401 cctgctgatt accctgtttg tgaattgggt ggtgaagccc ttctccatgg ccttctttgc
     5461 ctgggtgttt ttccggtacg tcttctcagc ctggatctca cccgccgagg ccgaccaata
     5521 catcgcgggc accattatcc tggctgcggc gccctgcacg gcaatggtgt ttgtgtggag
     5581 ttacctgacg gacggagacc ccgcgtacac gctcgtccag gtggccgtga acgacctgat
     5641 catgctcgtg ctgtttgttc cgattgtcgg gttgctggtg agcggcgcgt catcattggc
     5701 cgtgccgttc gacgtgctgc tctactcggt ggcgatcttt atcgtcattc cgttggtcat
     5761 cggcgccggg ctccggctct ggctggtgcg ccggtatggg ctgcggtgga tggaagaact
     5821 gtttctgccc cgattcgcgc cggtgacgat cgcggcattg ctcctcacgt tggtgtgcat
     5881 cttcgcgttt cagtcgcaaa acatcctggg gaagacggta catgtcatcc tgatcgcagt
     5941 gccgattctc ctgcaggtct acatgaattc ctcgttggcc tatggcctga tgcatcgctg
     6001 gcgggttccc tacgcgatcg ccgcgccggg agcgctcatc ggtgccagca attttttcga
     6061 attggctgtg gcgaccgcca tcgccctgtt cggaccggag tccggtgccg cgttggcgac
     6121 ggtggtcggg gtgttggtgg aagtgccggt catgttgtcc gtctgcagcg tgtgcaacaa
     6181 gaccaggcat tggttcaatc aggaggtcgg cacatgaaag cacgagtgct atttctctgt
     6241 accggcaact ccgcgcgcag tcagatggcg gaggggtggt tgcggcatct ggcgggcgat
     6301 cggtttgagg tgttcagcgc gggcactcac ccggttggct tgaaccctgg ctcggtcgaa
     6361 gcgatggccg aggtgggaat cgacattgcg ggacatcggt caaaacatgt ctctgaattt
     6421 ctgacgcagc cgttcgatta cgtcattacg gtctgcgacc gggccaaaga gtcctgcccg
     6481 acctggccgg ggcccaccca tctgttgcat tggagttttg acgatccggc ggcagtcacc
     6541 gactgtgacg aagcccatcg acagctgttt cgacgagttc gcgacgagat tcttggccag
     6601 atcaagggtt tcctggctca ggaggaaaag tcactcgtct caggtccttg ctaggtgacg
     6661 gcgccgccgc aggaggaacc ggagccggct gtgcacccgt agcagtggtt cctcgtcacg
     6721 atctgccgat ggtgcaagcg tgcgggatcg aagtctcgaa tgtgttgtgg ggttccctgc
     6781 gagaccggca attccagcat ctgattgaag tcgcaatcgt agagggtccc gtcccatccg
     6841 accgacaggg tgtagcggca catgacgcca gctgccgccg ccgggttgaa ggccgcggca
     6901 agccgttcca tgtagccttc gtagttgccg ctttcgatca ggaactccag aaatcggctg
     6961 atgggcatat tggtgatggt gtacaggcgc gtgaaattga cgccgtgacg ggaggccagc
     7021 tctttccgga acttcgcctc gatcccttcc tgcttcggcg gaagaaaggc gcccaccggg
     7081 ttgtacacca ggttgagcgt cagtccgctg ccctctttgc cgtagcccag ccggttcaat
     7141 aaccgaaggg actccatcga tttgtcgaaa ataccctcgc cgcgttgcgc atccgtctgt
     7201 gtcgcctgat aggacggcag cgatgcaatg acctccacgc ggtgctgcgc gagaaactcc
     7261 cccaggtcgg cttgcgaagg caggagcagc acggaaagat tgcagcgatc cagcacctga
     7321 cgtcccaata atcgggcctg ttcgacggcc cagcgaaagt gcggattcag ttcgggtgcg
     7381 ccgccggtga tatccacggt gggaatatcc gtgcgagcta gggcctggag acagaggtcg
     7441 agggtctctt tcgacatgac ctccgtccgg tccggtccgg cgtctacgtg acagtggcga
     7501 caggtttggt tacacagctt cccgaggtta agttggagcg tcgtgatgcc ggtcgcgtga
     7561 agcggataga gctctaccgc attgagggcc gtctcgaacg gctgacaggc cgtcgtctgg
     7621 gccaggagtc gcagctgctc cgatgcggct gcgagcggat ctcgtcgagc gagcaggctt
     7681 agacccatcg gatcgatccc ttcctcatcg tcaggggttc agcaacaacc gccggtcggg
     7741 ctacaggcga cttccgtgcc gtccacacct gccgaggctc gttggtagat cgtgaagtga
     7801 gggcgatagg cctcctgtgt caggacctgg agggtcttgg aacaaatttc caacggcacg
     7861 ccgcggtagt actggtgatg atcatcgtcc tcggcactaa tgatcggtcc ggtgagaatc
     7921 gcataggcgc cttgccagac gcagggtttc tgttccggat ggattgcaca ccagcgtgga
     7981 tcggtggcag cgagtgtgac gggcgtaggg ccgagaagga acagcgaacg aagcggctcg
     8041 ctctgcaaca gttgcgcgcg ttgggcgtcg atgctttggg gtacgccgcg ttcgtaccgt
     8101 tggcccaatt cgtccgtgac gctcgagaag gggccccgga gcgtggcata ggacgaggtc
     8161 ttttcgtcgg cgatggctgg aaacttgtac ccggtgagcg tcaccgagag gaaatggatg
     8221 ccgtcgatcg actgccaggg cgtggacttg atctggtgga cggcgcgaaa tcccgcatcg
     8281 accatcccgc cgatgtagtc ctgcacctgc aaggcgccgg acagacagtc gccccacttg
     8341 gctgcgtcat gcaccaggta ttgcggcaca acctggtccg acacgatgtc ggaaatcgta
     8401 aaccggccgc ccggcttgat cacgcggtac atctcgcgga agaccgttcg cttgtcgggc
     8461 gctagattga tcacacaatt ggaaatgatc agatcgacgc tggcgtcctc caccggcatg
     8521 gcatcggcca tccccttgcg gaactcgata ttcgatgtcg cgtagcccag gtttccggcc
     8581 acgatgggcg cattgcggcg ggcaatctcc agcatggtgt ccgtcatgtc gatgccgatg
     8641 acccggccgg tgggtcccac ccggcgcgcc gcttcaaaac aatcgatccc gccgccggag
     8701 ccgatatcca gcaccgtttc acccgattga acggtgttca gcccggccgg agtgccgcag
     8761 ccgtaggaga tggtcaggac ttcttccgga acaaacgatt tgaggtcggc aaagtcatac
     8821 ccggtcgggc aacacatctg ttcgccggtt gcagccgccc gcgcatagcg gtcgctgact
     8881 ttctgggtga tatcgtcgat ggccataagg tcctcggatt gggatagggg aatgcaatcg
     8941 gattgatcta tcaagagaag aggcggcagg tcaacatcgg gaaacggggg cagccggttc
     9001 gactgccccc gcagatcacg agcctaggca tcctgtcctg actactgcat cgaggccatc
     9061 ggcttctcga cgatgtcgct cttcatcggg cccaatgcgc cgagcagctc gttcttttcc
     9121 cgttcaggca ctttgaactt gtttaacgtg gcgacgaggt ctcccaccag cgcgtcgaag
     9181 tcgctgctgc tgacccccat accggcatgg gtgctcttca tatctcgtcc ggtgtaggtg
     9241 caaggcccgc ccgacgcctg acaaatttga tcgaccagca tcgacttcag gcgcgcgatg
     9301 ttggcgttgg cgaacttgcc gttgatgcgg gtgtcggcgg ccacgcggcc gacgaagtcg
     9361 tccaccactg cggtaatggc cgttttgccc cccagccggt cgtagaggga tttttcagct
     9421 gctgcggtgc cggccggtcc tgagccgacc gtattgcagg ccgcgacggt cagcgcgagg
     9481 ccaaggatca aagaaaggga tgccattcgt tgcgacatga gagtacctcc tcgttatggg
     9541 cctgactatt gggccgggtg tgtctcgaca tcgaccggct ctctgtctcg aactacgaag
     9601 tggaaccgta gttggatttc ccgggaacga aaaaactccg ttgtgccccg attagtccgg
     9661 tgctagagtt tcggcggatc ggcgcaggta gagggaatct aattccccgg gcagactcgt
     9721 agctcttgac gaggatgcgc atgatccaat cacaggctgt ggcacaggca ccttcgatac
     9781 atgaccagga atggacggga cttctcgcgc gcattgccgc cggagaccag cctgcgcttg
     9841 cggagttcta tgatgcgagc agtgccaagg tgttcgggtt ggcgatgagg atcctcgcgg
     9901 atcgtacggc tgcagaagaa gtcaccatgg atgtgtatac gcaagtgtgg cgccgggcct
     9961 cgacctacga cacggagaga ggcacgccgg ggagctggct gatgatgctc gcgaagacca
    10021 gggccatcga ccgtttccgg agcagttatc tggagcgggg gcggcaagtc ccgctcgatc
    10081 aagcggcgga gttgccgggt gatgcggcga ctcctgaaca atacagtgcc gacctggaac
    10141 ggcagcggct ggtgcaggag gccatggcga gtctctccgc cgagcagcgt caggcgattg
    10201 ccttggcgta ctactggggt ttgagtcaga gtgaaatcgc cggtcgcttg aggctgccgt
    10261 tggggacggt gaagacgcgg atgaggctgg gcatgatcag actgcgggag gtcctggctc
    10321 cgcacgggga aggggtgcgg tcatgaccga tccacgccac aaggaagaca tgtcggagct
    10381 tgcggcactc tacgccttgg aagccctggg tgatgacgag cagcggcaat tcaagcaggc
    10441 ccttgagaca gctcctccgg aagtccggca gcaggtcgca gcgtttcagg aggtggcgca
    10501 ggagctggcg ttcagcggtc cggccatcgc gccgccggca tcgctcaagg ccaagctcat
    10561 ggcgcgcatc gcgcaggaac cgcaagaacg ggaaggcgcg agggacttta cgtgcgtccg
    10621 atcggggcag gtggcgtggc gagacctggg ccctggtgtg tccatgaaac tgctgttcaa
    10681 cgattcggcc ggagcacggg ccacgatgct gctgcggctg gctccgggtg gaaaactgct
    10741 ggcgcaccgt caccggcagg tcgaagagtt ctacgtgttg gaagggaact gcatctgtgc
    10801 cggggaattc ctccaggcgg gggactacca ccgcgccgag gccgggagcg tgcatcccat
    10861 cacatcgagc gagcagggtt gcctggtcct agtgatgacc tcgacacaga acgaaccagc
    10921 cgggtaatca attcactcct gttgctgttc ttttcgcgat cttccgaaga cccgccaata
    10981 gatcgtcacg ttcaccagca ccaccgctgt ccccagcaga atctgaatga tgctggtcag
    11041 ccctgccgga tacatcagcg gcaggaggta gtggccaatg aagtcggagc cataggctga
    11101 ctctccggcc atggcccgca gagtgtgttc aatcggggtg agcgggcaga tccaaccggt
    11161 gtactcgaca atcgctcccc agattgccgc cggcagatgc acccaggcaa ggcgaggcca
    11221 tttcagcagc aacagcccac ccgtcacgac gaagagcaca aaggccaggt gtagcagcag
    11281 cacgagatcc gcgccgattc ggtaccacat gtgcggcagt ctgaagaaac ggcgcgatga
    11341 aggcaatcag gagacggcga gcggagccgg ttgccgcgtc cctacatctg agcgggaagg
    11401 tcatagccct gcggcggggc ggtggtgaag ggaaggggca actgcgcggt cggtaatgtc
    11461 acggcgctgc gggcccagac gatcaggtgg tccgggtctt cgagaatgtc ggtggggact
    11521 tcgtaatact gtgcgagcgt gtgcttggcg ttcgggcgga agggatgcat gccgcgcgcg
    11581 cgatagagcg cttgcgtcac gtcgttggtt ttgaaataga gccgaccctt gtggatgatg
    11641 ccgaagaagg tggtccgttg gtagaggcca tagccgccga acatggcttt ccatgtgagt
    11701 tcaggcatct caccgagttg atccaggacg aagtctttga agctgtcgtg gcgcatgcgt
    11761 cgagactgat tgggtggacc gctgctcatg tcggccgtat cgattcggcg cactctgcac
    11821 cgatgactca attatttcaa ctgccgggtg gcgaggggtt ccgttgtgag tccagggggt
    11881 ggtcgtcatt cgaggctcaa gcgtgagggg gaggctttat agtatacgga gccgtgcgag
    11941 tcgttgaacg tgtggaaggt ggaggctgaa accaggatgt gcggtctctg tgctgcagca
    12001 ggcctcgtcg cgcgtgaacc cggtcgcccc gtggatgcgc tattgcatga tttgagcaca
    12061 tcccacgagg cgcaagcgat tcaggggtct cagcggcagg aggctaggac accttgatct
    12121 cgatcgattt cggttttgct ttctcggatt tcgggagatg catgtggagc accccgtctt
    12181 tgtagtcggc tgtgaccttg ctcccgtccg catcctccgg gagtgagaag ctgcggacga
    12241 aactcccgta ggcgcgttcg atccggtgat gcttctccct tttctcttcc tgctcgtatt
    12301 tccgttcgcc ggaaatcgtc agcaccccgt tgtccacggt gagtcgcacg tcttcttttt
    12361 tcatggccgg caactccgcc ttgatgcggt attcattctc atcttccacg atgtccacca
    12421 acggcgacca ctcggcaacc gtcagggctt ccttgccccc attcccggtc gatgcccgat
    12481 gtcccaggag ggtcgacaat cggttttcca gttcgtcccg ttctttcaaa ggattccact
    12541 gaactcgaaa cggatcccaa cgtatgagtg tgctcatggc ttcatctcct cgtatggtcg
    12601 aaatggcatc atgacgactc gatgtcggtg cgattagtgg cccgctgcgg cagccagctg
    12661 acgttcggcg tttaaccagt cctccagagc ctgcccgtct ttccgccctc gtttctcata
    12721 cagttcgtag gcgcgtaggg cgatcgcagc ggttcccttt acggcctgcc ccgcgtgcgc
    12781 ggctttgtca tggctcgggc tcgtgtgggc tgtgccggcc cgctgcgggg tggttcgact
    12841 gacattcgtt tccattatgg tgcacctcct tgttgagccg gctgtgtagc ggctgaaacc
    12901 atcagaggct ctgccgtccg ccgctaccct tctgtcggat ctcggcggac gaagagcggg
    12961 tctgtgtgat gaagaaaagg gttaggcctt ttccttcacc gtgagatcgt ttttcacagc
    13021 cttcacgccg ggcacttgcc aggccgacca agaggcttcg gcgctggttg ccaggtcctg
    13081 aacttcgccg ctgagcgaga cgacgcctgc gttcgtctga gcatgaatgc cggctttctt
    13141 taggtgtgcg tccttctcca tatgctcgtg gacgcgggcg gtgatggcgt catccttgtc
    13201 gtccgtcgcc tcacgtttgg agggtgccac gacctgcaga tcgttcttca cggttttgac
    13261 cccgtcgatc cctttggcaa tcccttctgc agcctgcttg gccgcatccg aatcgacctt
    13321 gccgcggatc atgactgcgc cctgggcggt ctccacgttg atctcgcttc ccttcacccg
    13381 ggaatcggca aacagcgcga tcttggtctt cgccgtaatc caggagtcat tgatgggtgt
    13441 cttctcgtcg gccttgccga tggcatgcgc cgatccggtg attatcgccc cgccaagaac
    13501 caatgctgcg cagagggtca gtacattgcg tgtggtcatc aagtctcctt aagcgaacag
    13561 tgaaagtgtg cggcgaaatc tgtttcgccg atggtgtcga ttcagggagc cttcgttccc
    13621 tcggcgtccg gcttcgcctg ctcatgccag tgggaagccc acttcatcag ctcggccttc
    13681 ttttcgccgt accgttcttg cactttgccg acaaaggtgt cgtaattgcc ggcgatctgc
    13741 tgcaagtcgt cttcggtaaa cttgccccat tgttgtttca gatccccttt gaactggagc
    13801 cattttcctt tgagttgatc cgcgttcata ccgcctcctt tcgtggtgtc agccatgaaa
    13861 cctcggctgg gtgtgactgt cgggccctta tacggtgaac ggttcagaca gcctgaaccg
    13921 ttgtcaggtg gttcgcggag tccgtcccga caccaggtgc accaccagca acacaacccc
    13981 gatcaagaac aagatccaag acatctggac ggcaactgcg gagaccccca aaagattgag
    14041 ggcgttggca atcaagccga ctactaagaa catcagcgcg tagtagagca ttgggcactc
    14101 ctttcatgga gtcaccggcg tgagtcgaac aatcctccgg ggcctcgacg aatgacggtt
    14161 gcgctccacg agcggaccgg cacatcggac aggcggcacc tcagagagac tctgatacag
    14221 gagcggcgga gactggggaa tcagtcacgc gtacgaagga acgggaagcg cgaggatggg
    14281 acagtcagcg gagcttcatt gggcacctct tcacgctaag cgcaatcggc agtgacgtca
    14341 agcactataa tgtgaggccg gaacgcggag gctgaagcta agtggaaacg cgagtcgcaa
    14401 gaattgggga gatgtctcga tgtacggcta ggggttttgc tagtgctgcc cgctcggtga
    14461 tggcggcaca agggctatag gcaactcacc acacgtggag gacgggcatg cgtacattca
    14521 tcattgcggc gatcgcgggg ttctgtctgt taacgggatt gtcgttctct gccggagcgg
    14581 gagaaaccaa ggcaaagatc gaaaagggca aaggggagct gaagggcgat ctcgaagaaa
    14641 tgaagggtga aaccaaggcg atgaaagaag agctgaaggg gaacgacacg aaagcagaga
    14701 tggagcgggc caagggtaaa ctaaagggca cgggtgaacg ggccaaaggg aagatgaaag
    14761 agttgaagga gaaggcaacg gactagcgca ggttcgtttc ccgcccgccg gctcgtcagg
    14821 gccggcgggc agtgtattgt gcgagtgctg ccgtgcaacg gctactgcgg tttgcgcgcg
    14881 ctcacgatcc tcaccgcaat cggcaacgag accccttgtg tcccgcgata ttcgttcacg
    14941 gccttgatga tgccccgctc ggcctcggcc tgttgctcct tcgtcaattt ggcaaacagg
    15001 ttttgaatcg gtgcggcgat atccatcagg ccgcgaaaga attcctccgc tgtggcatag
    15061 gtcacctccg ccgtgaattc ttcatcgtcg agcggcttga ggccagcccg ttcgaacatg
    15121 ccggccagat cgcccggctt tgcaaggcgg aagatgccgg gcgcgtcagg gtccggcggc
    15181 ggcaggggcg tgatggtttt gatcacgtcc attgggatgc gaatgaacgg attcttgtcc
    15241 ggcgccgacc agacggcggc cgcgacatag cctcccggtt tcaacacccg ggcgatttct
    15301 gcggcagcct tcggaatttc aggcaggaac atcaagcaga accggctgat cacggcatcg
    15361 atcgagccgg tctcgaacgg cagtgaagtg acatccccgg tacgaaacgt cacgtgctcc
    15421 aggccgagcg tcttggcttt gcgcgtggcg accgccagca tggattcagc gagatcgatc
    15481 ccgaccacgg tgccatccga tcccaccact tcaccggcca gcagcgccgg atagccggtg
    15541 cccgatccaa gatccaacac ccgaagcccg ggccgcaccc gcgcatcggc caccagccgg
    15601 tggttgatga aggccatgtt gcggttgaag aactgatccc atttgtccca accggcggca
    15661 acgcgattcc agtcctgacg ctgggcttcg atcacagcct ctggtgtttg tgacggcatc
    15721 ggagcgactc cttgttggac aaagtagaga ggaagattat ttcacgcgtt cgaaggtaaa
    15781 caccttcggc acgtcttttc cggccttgct caaggtccag gtctgggtga agtgatcgtg
    15841 gtctttgacg tcgatggtga gctggcgcat atgcacatcg gtcggattgg tcagatttgt
    15901 ggcatccaca aacgcaaacg taatggcctt atcgccctct ttgatcgcgg cggcgcgcat
    15961 gcgcggctga ttgttgaggg agcaatagtg cgtcaacatc agttggtcgc cgtcgctata
    16021 gtacatggtc gtcatcgggg gggctgtcgg gaggagtgag aatttcggtg agggtggtgc
    16081 cgcctgaact gagggtaaag gcgatcttgt gggctttccc ttccgcgtcg gcccccttcc
    16141 agtctccgac gaggaacttt aagggactca gggttgtatc tggagtatcg gcggcctggg
    16201 cgaagagcgg gacgaggagt aggacgaaac cacagagagt gcgaagaatt ccacgcggca
    16261 tgcaaaccct ccatcaaaca agaagatgcc gaacacaagt cagtcaagca tgtctccaag
    16321 gtcgagatca agactttttc cgccgctgtc gcagaacggg acagcaggaa cggcagagtg
    16381 aatcagaaat gcacgtataa tgacttggag tgaggggaaa gacaggaggc gcgttcaggc
    16441 gagaatggcc gggggagtgg gagcgtccgc catgaatttg atagaacagg tctggcgata
    16501 gttttcctga gacacgaacg acaggccggc ctatgccgaa taactccggc gcatcgatct
    16561 cgaaggcgag tcagctgcgg ccgtggagcg ggcgctgcaa atcacgccga ataacctcac
    16621 cgtgcatctc catcgcgcgc gccaggccct gcgggccagc ctcgaacaga cctgcgggct
    16681 ttgcaccaga caaggctgtt tcaactgcac gtgcgagtag ttcctccgcc taccggtaga
    16741 tgtgaaaaga atggtcggcc ccctgtaata attctcgcct tgctccgtct gtaggggtga
    16801 acggctgatt tcaccattca cagggaggac acacatggct caccaccatt cagcgactat
    16861 caccgtgccg cgccacgcag agccacgaga tcacgatcac cacacgaacg ggcatgactg
    16921 tgtgcaggct cacggcgatc accttggccg tcatcagcct cacgagcatc aggaagcgaa
    16981 ccaccaggca ccctcgctcg atcgaacagc gctgatggcg accctgcatt gcttgagcgg
    17041 ctgcaccatc ggcgaagtgc tcgggatggc gatcggcacg gcgttgggct ggagtaattg
    17101 gccgacggtc ggcctcgcgg ttgcgctggc ctttctcttc gggtatgcca tgaccttgta
    17161 cccgttgcgg cgggcgggta tggcctgggg aacggcgttg gggttggcct ttgcgtccga
    17221 taccctctcc atgacgacca tggaactggt ggacaatgcg attatggtga tgattcccgg
    17281 tgcgatggac gccgggttgc ctgatccgct gttttggggc agtctggtct tgtcccttct
    17341 tctggccgga gtggcggcat ttcccgtcaa ccgctggttg atcgcgcggg gcaaggggca
    17401 tgcgctggtg catgggcagc actgccactg agacggttcg gctgcaaccg tcgatccgct
    17461 gctgcgacat gcctggctgc tgatgagggg cagccaggcg ggcgggctcg tcgttcagtc
    17521 cttcgacgta cttcacagta cgcctcagtc cctcacgact ccgcgcgccc gtctcgcgac
    17581 gcggcttgac gatgtcgcaa cgaaccgtcg ttagcccgga ttattcatga atgtcgtcgc
    17641 gaggcgttcg agaccgaagc ccgccggggc ttctcgcaag cgaggctgac gcaggcgtac
    17701 ttgcagtccg tcgaggaggc cgaacgagaa cagccccgcc gggcgagaga atcgaacgac
    17761 ggagcaggta ttcatgaata gtccgggcta gggttttgat caatgtgatt gaggagcgat
    17821 tcagcatgac gattgggaaa tatatgcgca tgctggcgat cgtggccacg gccatggtcg
    17881 ccttcgcgct ggctgggtgc agccagttcg agccgcgcga caagcgattc tattaccggg
    17941 cgctgtggaa tttttcgttg cgggaagatc tggccgaact cgacagtgag ttcaacggcg
    18001 tggacttcgg ccattccaat ctgtacgaga atctgttgct gaccggcggt caggacgtgc
    18061 cggccattga ggagcgggcg cgcaagcaga cgctcgcgtt tatcgcgacc aaaccccggc
    18121 tgaacccgaa cgaggaggcg attgcgccga cctatatgaa gctggcctgg cgcgcgcaga
    18181 acacgttcga cgaagcccat gccctgcacc gcgccacgta cgacattgcg gtgtcggacg
    18241 agccggagaa ggatcgggcg atccgcaatg tgttggccta ctataaggac agcgcctacg
    18301 cgatcacctc gaagcgattg gatcaccatc ggctggacca gtttccctat tccaaggcct
    18361 tcaggacacg cttcccgctg ttcaacgcga cgatctggtc gtaccactac ttgcaggtgg
    18421 ccgtgtacga cccgttgcag gccgcgcgtg acctggccgc caagacgcaa gcggtccggc
    18481 cgattcttgc aacctaccgg cggtatctcg aacagccgcc ggtgcagtgg accttcatgc
    18541 cgctcacagc ggaactgagt ccgcagtttg ccgcgcggta tcccgaattg gccaacatct
    18601 tcgacaatct ccatatgttg cacgacaaca tcagcgatat cctcaccagc gagcggcttc
    18661 cgacctggga ggcgaagcgg gttgaaattt atcgcctgct gaatcactac tatctggcga
    18721 gtgcggatgc gaccaatccg atgatcgtgc aaggacagga gcatcaccat tgaagctgag
    18781 aggtaagcga ttccttgcgc gcttgctcgg cgcggccctt gcgctggtga ccacaccgct
    18841 gttcgccctg gaccatgaca acctcgatcc caaccgtccg atcggaatgg aggatgccta
    18901 tgccattccg aaaggggaga tcggcctgga gggcggggtg aggttcaacg accgtcgaga
    18961 aggccgcacg caggtgacgt ttcaaccgca aatcatctac ggggccttcg ccaatacgca
    19021 gatcgaaatc caaggcgatc tgtttaccga cccgcgctcc atcgtggggg cgaacaagtc
    19081 cggcgatttg cacctggggg tcctctacaa cttcaatacg gaaacgttga tgctgccggc
    19141 gttggccgtc cgggtcgagg cggatctacc caccggcgtg aattccaaag gcgtggacac
    19201 gcagctcacc ggcatcctga cccgctcgtt cggacggctg cgggtgcacc tcaatgcagg
    19261 ctataccgtg atgggatcgc cgcaagggca ggagcggccc ggcgcctatc gggcggtcgc
    19321 ggccgtcagt tacccattgg gctatcccac cagttttcgc gatacgctga ttgccagcgt
    19381 ctatacccgc caatcggacc tgcgcgggca acagaatcac accggcattg aagtgggcat
    19441 ccggcaccaa ttgacctcgc gcgtcgtgct cgacggcggg ctcggcaccg agttcgtcgg
    19501 cccggcggat cgtgctgcgt tgctgggggc aatgggggtc tccgtaggat tttagaggtc
    19561 gggggggagg cgtccggcgt tgctctgtgg agcgaaggga cggggcgtct tagcgtgcca
    19621 ggctcagttt cgccatgtgc cagagactct ccgccaacca gcggctgtct ttgaacagat
    19681 gggccagttc ggacgtgagg atgaagaagg ccggataaaa cagatgcgga atcgatttac
    19741 tccaggccag ggccggttcc accagcttca caaagacgga gcgccacttc cagatcacgg
    19801 ccatggccgt cacaccgagg cacgcgccag acaggatgtc cgtcggaaag tggaggccga
    19861 cgtagatcct cgggaggcac acaatcacac ccgcatggag aaaggccagg gcgcctacga
    19921 acggggacac caaccaaagg ccggcggcaa gggcaaagaa cagagacgca tggtcgctgg
    19981 ggaacgaact ccacccggcc agaaaatcgg ccggcatggt gtagggcagg gtgaaatcca
    20041 gtccggccgt gtgcaccgga cgtgcgcggt aggggaggac gagctgaagt gcgcgggcga
    20101 ccgccatcgc cgcgaaggct gcgaggatcg tcaggaggac ggtttgtcga tggtcctcac
    20161 gatggggggc gcgaaaccag gcccaccaga tgagcggcag agagagatac cccttcagcg
    20221 catcgctgtc gctgatcgtc atgatcaggc tgtcgaaggc ccaggaccgt tgggcaaatt
    20281 ggttgaagaa acctaagacg gtcgtgtcga atccgttcat gtacgcgtca tctgcagggt
    20341 ggagtccttg tcgcgtgagt cagaggtcat gaggctcggc tcatgccggg gcgcaggtgc
    20401 gcggcagcgg caagatagcg gaggtcaggg ctgttgtaaa tgcttataag ccagaccggt
    20461 gaaaatcgaa aaatgttttc gtcattcgag cgcccgacgt gagactcgcg tccgtaccgg
    20521 ccagggccga tgtgccctgg tcaaaggccc tccgggatgg attggatctg cccgtggaat
    20581 ggggtcgatc accgccttat tgacggccat tcggccctag gcagataatg cgcccgtcgc
    20641 caggaggcag gcatgggacg ggccggccgg atcgcacgcg gaatcatggt acttgtcagt
    20701 gtcctgttgg tcgccgcctg cggcgattca ttcaatgacg cgtattgcga ggcgaacaac
    20761 caccgcaatc agcaaaccgt cgaacacctc accaacgtcc tcgatcaaaa acgcaatcag
    20821 cccggactct atctcgcgcg tgcccgctgc cactattttc tcggcgccta tgcgcaagcc
    20881 ttgcaggacg cgtcggaggt cattcgccgg ctgccgaaca aggccgatgc ctatctcgtt
    20941 cgcgccaagg caggcaagat gctcgggcag attccacagg cgctgcaaga ctacagcgcc
    21001 gccattaaat tccagccgac cgccgaagcc cactatggcc gcggattgac ctacgtgcgg
    21061 gagtatcagg ggaaggatcg cgaagccctg gaggatttca ccgccgccat tcgccttgat
    21121 cccaaacatg tcggcgccca tgcattccgc gctcagatct acagccgcca cgaacagttt
    21181 cagcacgcac tggcggattc ggagacagtg ctcaagctcg atcccaccac ccccaacgcc
    21241 tactgcaatg tcggctttgc gcactatgcg ctggggcatg acgccgaggg ccgcaggttt
    21301 ctcaccacct gctaccagaa agatcccgat ccccaaacgc gcggctatta cgaaacggaa
    21361 gtgaacaagg tgctgtatgc gcggaagccg aagcggaatt ccggcgggag ctacgtcgct
    21421 gaaggcgggg tgaagacgcc ctacgaccgg gaaatggaac gccagcagaa cgtctacgag
    21481 agcctgcgca attcagggtt caatgatcgg gccgaggctt gccggacgga tgggtcgaag
    21541 tgttgagggg gcgggtggcc tgacgggtct cctgtgctcg cgcaacgcgc ggcttcggaa
    21601 ggccctcgtt ggacggcgcg cagtgggggc tcactcggga cgccccttga gggagtggag
    21661 gggaagtgga aggagcattt gacactgccg gctcacctcc tcgccggcgt ccacaaacgt
    21721 gacgctcctt attcgtcgcg tcgtggacct cacggaacgc gcgtcctcag aagggcctcg
    21781 ttcgacgtgc gcagttggga acggcgtcac gccacccctt gaggggagga ggtaagtgga
    21841 gggagcatta gattttaacg cgcggcctcg gaaggccctc gttggacgcg cgcagtgggg
    21901 gctcactcag gctacccctg agggggaggg ggtaagtgga gggaccatca gatcatggca
    21961 tgcagcgtta agagtgaaga aggagcagcc gccacgtcct tcttgctcgc agaccgcgca
    22021 tgatcagaat atgctcggcc catgcgcgca gtgaaggaca gtccggctac tcccttggga
    22081 caaatgggca agcttggaag gatcatttcc tgggtcagtg ctcggtttga tctgttttgg
    22141 aggatggacg aggtttattt ggaaaagtag aatgccccag ttaattcccc tgaataataa
    22201 aaatgggcga ggcaatccca aaagaggaaa gcgagtgggc gcggagggag taccataaga
    22261 acgtggttcc gaatgcgtgc agtgaagatt ccaattcttg gaattcagag gagggtagac
    22321 gatggcgtgg ggaccggatg cgaatacaga agatgaattg aaaaagctgg cggccgcgcg
    22381 tggatatttt cagcaagagt tccccggcgc ctcgattcgt gattcgtacg atgccggcaa
    22441 aatggcgcag gtgtttcgga tcgaagccga tgacgtcggc gggctatgtg atgcggtagt
    22501 ct
//
LOCUS       DAORQN010000015        21431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_169_length_21431_cov_10.658308, whole genome shotgun sequence.
ACCESSION   DAORQN010000015 DAORQN010000000
VERSION     DAORQN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169_length_21431_cov_10.658308"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..1729)
                     /gene="der"
                     /locus_tag="PK614_02250"
     CDS             complement(335..1729)
                     /gene="der"
                     /locus_tag="PK614_02250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRB81064.1"
                     /translation="MLPSRTMSRTKQTRGPVAPLFTLEGGRLPIIALIGRPNVGKSTL
                     FNRILGTRAAIVDDVPGVTRDRNYADSTYRNRRFRLVDTGGLDPTASEGMLSLIRQQS
                     QLAIAEADILVLVLDARSGLTPADEEVVQTLRGSDKPVYYVINKIDTPKADPLVADFY
                     RLGQEQLYPLSAEHGIGVAELLDDLFSHMAEPLDEEATSDTPRIAIVGRPNVGKSTLV
                     NSVLGEARVVVSDVPGTTRDPVDSVATFKDRQYVLTDTAGIRRRGRVERGIEGYSVAR
                     SLRAIGRSDIAVLLLDAEEGVTEQDTKIAGLVLKQGRACILIVNKWDLKTDDVHAREA
                     YKQDLERRFPFLAWAPVLFISALEQDFLRGLFALIDQVYFSFCKRVPTGPLNQFFQGL
                     LEEHPLPVRKGKPAKASKSAFMTQVATRPPAFALFVGHPDNMTPAYLRFLENQIRKEY
                     HFSGTPIRLMTRKK"
     gene            1790..2476
                     /locus_tag="PK614_02255"
     CDS             1790..2476
                     /locus_tag="PK614_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRB81065.1"
                     /translation="MLSNRSEAAHQSVDWSRIDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAAKHALTFEAARDRLMAMYRSVEGELAWTDLHYWSERVDIDVVAMHRELDHMIGF
                     LPDAEEFLSSLRRLGKRVTILTNAHRAGVDVKTAKTGLDRHVDRIVDAFEVGWLKMRS
                     EYWPTCRQLVGFDPARALYIDDDEQCLAAAEDFGIGRIFHRSRSSSQASAVPSARFTS
                     IEHFHAILPG"
     gene            complement(2523..6467)
                     /locus_tag="PK614_02260"
     CDS             complement(2523..6467)
                     /locus_tag="PK614_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB81066.1"
                     /translation="MSSSQSFPDREPPSTSPLFKPIDSARDSSIALQRVTLLYEAMPA
                     ALVAGLGCALALIAVNWHVVPHDRLLIWLVYHLILAAGRYGLARSFTANKPGPANIER
                     WERAFLVGTTLAGLGWGASALLLFPESSPAHQVFLAFVLAGITAGAASTLAARFDAFL
                     SFSLPMLTPLILRLFALDHEQALPMAICTMLFSLLMVYTAYRTSNTVLETLRLKYHNA
                     QLVDQLTGQLQEGEQMELLLTVKTEHHRFIMEYAQDIIYRTDRSGRFTFINPAVILLL
                     GYHETEMLGHRALDFVHPDYRRPTERFYLRQFLRKTASTYCEFPLMTHTQQTLWVGQN
                     VQLLQRDQEVIGFQAVVRDISVRKQTEEALRVSQERYRALFESSPEMLLTVDARGTLL
                     TVNTTTAAELGYEADELIGQPVAGIVHPDDHGSMQQHFVDCLRRPGEVLRWNFRKMRK
                     DGSLLWVREAARAVRNSDGQFDIIIVCENVTEQKGIEDALTQTRQLLESIVEHIPHMV
                     FLKDAQELRFVQFNQAGEELIGLPREALLGKNDFDFFPLEQAEFFTARDREVLTGGTM
                     MDISAEPIQTKDHGLRWLHTKKLPLYDQAGMPRYLLGISEDITDRKQREEAERLRLAK
                     LETQQAVLHELAEDPAIHSGHPQQAFPAMTEHAVNTFAVERAGIWLFGEHQSALILQD
                     LFEATPKHHLQGTTLSTGEYPAYLHALETEPYSLAAHDAQTDPRTSELTPSYLTPLGI
                     VAMLDAPIRRHGRVVGVLCLEHIGPARVWTTEEQTFAASLAAMATLTLEASDRRQAQE
                     ALEQHVRERTGELRRTTAHLQTIIEESPLAMIELDQAGLVTTWNAAATALFGWTKDEV
                     LGKELPYVPPGQEEVSEELWTAVMSGAAPRNLELCRQKKDGALVDVNMWGSLLQSPTG
                     QAVGSIGFFVDITKHKQLEDQLRQAHKMEGIGRLAGGVAHDFNNLLTVINGCATLVLD
                     QVRAEDPLRRSLTEILTAGQRAAALTRQLLAFSRRQVLTFQVFDLNEALSSISSMIER
                     LIGEDISLMRNLDSQPCTIRADRGQIDQVVLNLAVNAKDAMPAGGTLTMATQALLITP
                     DSPVPHPALLPGAYAHLSIRDSGQGMDRTTLSHIFEPFFTTKEEGKGTGLGLATVYGI
                     VKQSQGFIFADSTPGEGSTFDLYFPLVEAPPLAATPTLPSRPHCGRETILLVEDQHAV
                     RLLLTQALSDYGYTLLEASSGQEALRLVAAARTPIHILLTDVVMPQMTGPALAEHLRR
                     RWPELRVLFMSGYAEGSVLPTFLAEPGTGFIQKPFLPTELAQKLRELLDPAK"
     gene            complement(6609..7223)
                     /locus_tag="PK614_02265"
     CDS             complement(6609..7223)
                     /locus_tag="PK614_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB81067.1"
                     /translation="MDWVLRGERFQTERRLLLTPVHGVVLEIGFGTGLNLPHYPTTVT
                     ALHTVDPAPLLPDRVAVRVAQASFPVHIQHVSAERLPYDDASFDYAVSTFTLCTIPDP
                     ARALRDIRRVLKPDGHFLFLEHGRSDDPLIARWQDRLNPLQHLLACGCNLNRRIDRLV
                     LDAGLRLEQLDRYCLPGVPRIGGEMYRGTARVNGQPPLHPSLET"
     gene            7513..8782
                     /gene="leuS"
                     /locus_tag="PK614_02270"
                     /pseudo
     CDS             7513..8782
                     /gene="leuS"
                     /locus_tag="PK614_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
     assembly_gap    8783..8792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8793..9916
                     /locus_tag="PK614_02275"
     CDS             <8793..9916
                     /locus_tag="PK614_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRB81068.1"
                     /translation="IVWVDGEPRPLPEEQLPLPLPETKNFKPSGSGESPLANLHDWLE
                     TTDPVTGKPARRETNTMPQWAGSCWYYLRFIDPKNAMQMVDPAKERYWMPVDLYIGGS
                     EHAVLHLLYSRFWHKVLFDAGVVSTPEPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDE
                     MIDQFGADAVRLYEMFMGPLESMKPWSTRGVEGITRFLDRVWRLMIGDEGALSPAVTD
                     AEPAPEQLRLLHCTIKKVTDDIDALRFNTAISQMMVFTNEMTKLEQRPRRLLEPFVLL
                     LLPFAPHLSEELWERLGQQPSAGQQPWPQFDPALVVSDRLTIPIQVNGKLRGKLEVDA
                     GASREQLEPLAKKEVAEWLQGKEPKKVIYVEKKLINFVV"
     gene            9984..10610
                     /gene="lptE"
                     /locus_tag="PK614_02280"
     CDS             9984..10610
                     /gene="lptE"
                     /locus_tag="PK614_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRB81069.1"
                     /translation="MVWLIIFFALSVSLPGCGYQFRVQGAGPTVGGAPEAATKRPHAP
                     RLAILPIANSTYEANFEVKLANYLRREFSAGAGAEIVGPSAGADLYLSGQIMQITLPT
                     LSFDQTTTFESRVEMLVSVRIEEAKTKKVVWYQVAKGTSEFFLTQDLQFNRVLQNRAL
                     EQAGRFVAEDLASRFLLFLDTGELDKALKRPAKAEVAPAGGTPPYGAR"
     gene            10631..11740
                     /gene="holA"
                     /locus_tag="PK614_02285"
     CDS             10631..11740
                     /gene="holA"
                     /locus_tag="PK614_02285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRB81070.1"
                     /translation="MSASVMSLELPQSLARSGVMPLYAVVGEEDYLRDQSVAALRAAA
                     LGPAADTGFNYDIFHGDDCSVEDVLACAAEIPVFAERRVVVYKSVEKLPAREGEKLLS
                     YFSAPNDTTTLIVVGVKLDGRMKWTQALTKGAVTVNCAPLRDAQLSAWIRQEATALGV
                     RIHEEATQLLKEVGSESLYAVKRELEKLAAYVPPDRTVQPADVEALRGTESGASVFDL
                     MNAIGAHDHGRALRILARNLENGEAPLRILGALVWQYRRLWKMKEQLKAGGREGEAAR
                     TLRMDPSRVRGFLAQFSDVQLTHTFRWFLETDSKLKGGSGSAPVRVMEDLLFRLCGQP
                     SKSASSATRVQPSPPVPRPSGSRPVSNVRTVTRKR"
     gene            complement(11724..11999)
                     /gene="rpsT"
                     /locus_tag="PK614_02290"
     CDS             complement(11724..11999)
                     /gene="rpsT"
                     /locus_tag="PK614_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRB81071.1"
                     /translation="MPVVHKSTIRRARQSEKRRLRNRSTISSVRSILRKVQDAIAAKK
                     PDEAKATLREATSALSKAVTKGVLKPNTASRRVSRLTVRVNAITASA"
     gene            12114..12833
                     /gene="pyrF"
                     /locus_tag="PK614_02295"
     CDS             12114..12833
                     /gene="pyrF"
                     /locus_tag="PK614_02295"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRB81072.1"
                     /translation="MMTRIDARDRLIVALDVPSAAEADALVARMGDQVRFVKVGLELY
                     TVAGPDIVRKLVDRGKRVFLDLKFLDIEETVRRATARVAAMGATFLTVHANRKALIAA
                     VQGRGSSDLKLLAVTVLTNFDGEDLREMGIQRSVQDLVTARAMLAAEVGCDGVVASGE
                     EPQAIRAKVGPNLVIVTPGVRPAGKGTDDHARVTTPTQTIAAGADYLVIGRPIRDAQD
                     PPAATAAILEEMQAAFDRRGR"
     gene            13002..13078
                     /locus_tag="PK614_02300"
     tRNA            13002..13078
                     /locus_tag="PK614_02300"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13036..13038,aa:His,seq:gtg)
     gene            13116..13490
                     /locus_tag="PK614_02305"
     CDS             13116..13490
                     /locus_tag="PK614_02305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB81073.1"
                     /translation="MTGGNKQVAPLAPRSSPAPVATTFQLRSFRRFPVQCSVYYSSDA
                     LQGTGVAWNLSLNGWRIDGTHPVEPGTVVTLCVFLPDQHPTVFIDRAVVRWSRGQEFG
                     IEVNEIKADEHARLEQFVTALV"
     gene            13654..13998
                     /locus_tag="PK614_02310"
     CDS             13654..13998
                     /locus_tag="PK614_02310"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB81074.1"
                     /translation="MAKSQTSRSHSRVPVSCFLYYLGEGLVGTGKVCDLSVKGWRIDG
                     DKPVTVGMKLTLRVFLPDQPKAIDVDGVTVQWVQGRVFGLETVRMSATAQARIHKFVE
                     SVLKESGSSRVA"
     gene            14223..14588
                     /locus_tag="PK614_02315"
     CDS             14223..14588
                     /locus_tag="PK614_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81075.1"
                     /translation="MEQQNRPRFIVECTGSRSEDGLLVWNGRMLDLSIPGWSRTGLKG
                     LQAGDLLQLHLHIPGQPRPLSVRLATIRWASESKLGVDPILMDADDQLRLNDFVSAHD
                     TTPPFSTDRKEQIVISDAG"
     gene            14888..16189
                     /locus_tag="PK614_02320"
     CDS             14888..16189
                     /locus_tag="PK614_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81076.1"
                     /translation="MTPGPQLPDATHTTPLTPETEAQVDQFAHTDILVGIPSFNNVET
                     IGHVVKAVSAGLAKYFPEARAVLVNSDGGSTDGTQQVVSQAVVDLKTLFIGDQQSSLH
                     KIITPYHGIPGKGSAFRTIFEIARRLKAKACAVVDSDLRSITPEWIELLVSPVFEQGF
                     DYVAPYYLRHKYDGTITNSIVYPLTRTLYGHRIRQPIGGDFGFSGQLAQHYLDKHVWE
                     SEVAKFGIDIWMTTEAIASGARVCQSFLGAKIHNPKDPAADLSAMLVQVLGAVFALME
                     DHYAVWGKTDGSNAVPLFGFQYEVGVEPVNVNVDRMISTFRQGLSDLGTIWDQILAPG
                     TRQSLRPLGTCSAQDFRIADNLWARVVYDAAVAYRNRVLPRDHVLKAFTPLYLGRTAS
                     FVLDTQGLTSSEAEGRIEALCQAFEKDKSYLVARWNEPHAG"
     gene            16247..16954
                     /locus_tag="PK614_02325"
     CDS             16247..16954
                     /locus_tag="PK614_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81077.1"
                     /translation="MKEFFEKGLLDPLELMARQVLAVLPSLLAMSIIFFAGLVVAWTA
                     SQALERLLRVVGLDRLFDRLGVTGALLRGGVKTDPSRLVGQAAYWLVMIFATVAALGA
                     LNLQPINDFAKSFLAYVPHLVTAGLILVAGWLLSNFVSQAVLIAAVNAGLPPARLVAT
                     CSRWGIQLLAVAMALEQLGIAQNIVVVGFGITLGGLVMAGAIAFGLGAKDLAKDYLER
                     GLSIRTRDGAPDDLRHL"
     gene            17002..17223
                     /locus_tag="PK614_02330"
     CDS             17002..17223
                     /locus_tag="PK614_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81078.1"
                     /translation="MTDKISDVDAEAKARRWLGGEAAVPRYPHRRQSPFVFNRSLVGL
                     VAFAVGLGFAGGLLAGLQCRRRPPVSAKE"
     gene            complement(17285..18115)
                     /locus_tag="PK614_02335"
     CDS             complement(17285..18115)
                     /locus_tag="PK614_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRB81079.1"
                     /translation="MTKAHILILTDLDGTLLDATTYSYEAATGALSAIQTLGATLILV
                     SSKTRSEMEPLRLRLDNRHPFIVENGGALFIPMGYFPFPLEQAAPRGDYEVVEIGTPY
                     VRLRTALKEIGRDLGCRLRGFGDLSLEEVSDLTGLSPAETLLATQREYDEPVVVESNG
                     MVWNRLSAAAATRGLRWTRGGRFYHLMGGNDKGVASRRLIAWYERRAQQEGRALITVG
                     IGDSLNDLPMLEAVTYPILVRKPDGSYDPDVQFPHLTRAEGVGPVGWNRSLMDLLPTL
                     "
     gene            18525..>19029
                     /locus_tag="PK614_02340"
     CDS             18525..>19029
                     /locus_tag="PK614_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81080.1"
                     /translation="MRSDKKSPLKSDKKVPPKVQKKGSIGFNKKSALLEDAITQMNAG
                     KYGRASSALKDLLALDPLNAEARRLFATLHLRLGSLMSARTAFESLAREAMERQDYWL
                     AESLLREYLTAGPRYVPFLEMLGRVYEEKGDVMAAVAEYGKAVEVLLEDPDTDHPNRA
                     SDLFVRIR"
     assembly_gap    19030..19052
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19072..>21431
                     /locus_tag="PK614_02345"
     CDS             19072..>21431
                     /locus_tag="PK614_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81081.1"
                     /translation="MAFRFAAMFDSVTGQVLQVTPQPPVVDSINETAHAPALIETPPG
                     TDSAVVMPWEHMEAASSDVPRQHEIQVSAVEQSPEVIAFTTTSSTLEAVTEDLLQAKP
                     EESLSVTLPVDSPQESEVQPQSESSVSAVTSAVEPVQAAGESDPPVLEVDPASIEPAE
                     IASASVAEVSTPTMDASAKTGPDSIATLSAHSAPMPWDQVEEAAGIIPPQPEGPAATP
                     ADIAPPPSGEVGLTPSVAESPAPSIEVLTQPSGHPIATAPASPAPMPWDQIEEVAVVI
                     PSPPSSPEPVIVDAESLPPTQSEVSTLPLTELAPFQPVEPEPPAAAAPMPFEEMDLQA
                     PVSEPIAPTIEVLTQASSHPIAQAPTSPAPMPWDQIEEATGAVDPRTETLMEPGTDEV
                     EKAIESPAPNQAVEVSQAAESVPSSRPSELTSSGLSWEEILAAVAAMQTSPAPAQTLS
                     DGAQKPASDAAVQEQTAGALTAPPAPPEEESPLFEATVPDIPADQAGAATTLSAPMPW
                     EQIEVDDVTIPRQEPEPEFGPAFAEGAVAVQDSTVALPAAPDVTEATVAGEACLAETV
                     ADRPVEPIATVTAELRILSPDAPTKAPEDSIRIPVEPYKAVEAPTPDTVPEAEQPAES
                     TIDRAVEAPLRLADSESVVATSLEEPPPASVEPVTEFAIAVSTDVTPMEPEQVTVVEP
                     QAPVGEAVAELPSEPVLPVEQPAEMLCAQEVMVEPAPEVVTESLVSAAAPSLAEEKPV
                     AEAEPVAPLSPAALEVTPEPEPEMVLVEQEQSSTPAAEVRDVDTVGL"
BASE COUNT         4079 a         5957 c         6919 g         4443 t
ORIGIN      
        1 gtcgttggta tggagaatgc gaccgtccat ggaaaggacg atgaggccgg tctcgaccga
       61 cgaggtcggt gcagcagggg tggcgaggtc cggttgcccg atgaacatag agataggctc
      121 ctctctgcta tggctgtcca ccgtcgggtc agcgcgttgg tttgtcgctg agcgtcgact
      181 gttgaaggga aaagcaagat gcgagccact gcggaaaatt gcgggaaagg aggaaaacta
      241 cgaaatgttc aaggaatcgt gagcgatgaa acgaaggggg cgaaggaaaa tgtcgtcgat
      301 tgtgtcgaat gcgtcgcgcc ccggcaaggc tgcctcattt ttttctggtc atcagtcgaa
      361 tgggcgtgcc ggagaaatga tattccttcc tgatctggtt ttcgaggaag cggaggtagg
      421 ctggggtcat attgtcggga tgtcccacaa agagggcgaa tgcgggaggc cgtgtcgcaa
      481 cttgcgtcat gaatgcggat ttgctcgctt tggccggctt tccttttctg accggcaacg
      541 ggtgttcttc cagcagaccc tgaaagaatt gattcagggg gccggtgggg acccgtttac
      601 agaatgagaa atagacctgg tcgatcaagg cgaacaagcc gcgcagaaaa tcctgctcca
      661 gcgccgaaat gaacagcacc ggtgcccagg cgaggaacgg gaagcgacgt tcaagatctt
      721 gtttgtaggc ctcgcgagcg tgcacgtcgt cggtcttgag atcccacttg ttgacgatga
      781 gaatgcaggc gcgtccttgt ttgaggacga ggcccgcaat tttggtgtcc tgctcggtga
      841 cgccttcttc cgcatccagc agcaacacgg cgatgtctga tcggccgatg gcgcgcaggg
      901 atcgggcgac gctgtagccc tcgatgcctc gttccacacg accacggcgg cggatgccgg
      961 ccgtatcggt cagcacgtac tgccggtctt tgaatgtcgc gaccgagtcg acgggatcac
     1021 gcgtcgtgcc cggcacatcg ctgacgacga cgcgcgcttc gccgaggact gagttgacca
     1081 aggtggattt gccgacattc ggacgtccga cgatggcgat gcggggcgtg tcgctcgtgg
     1141 cttcttcgtc gaggggctcg gccatgtgcg agaagagatc gtccagcaac tcggcgacgc
     1201 cgatgccatg ttcggccgac aggggataca gctgctcctg cccgaggcga tagaaatcgg
     1261 cgaccagcgg atcggccttc ggggtatcga ttttgttgat cacgtagtag acgggtttat
     1321 cggagccgcg cagggtttgt acgacttctt cgtcggccgg cgtcaggcct gagcgggcat
     1381 ccagaaccag gaccaggata tccgcctcgg cgatggcgag ttgggattgt tgccggatca
     1441 gggacaacat gccttcgctg gctgtggggt ccagtcctcc cgtatccacc aggcggaagc
     1501 ggcggttgcg ataggtcgaa tcggcgtagt tgcgatccct cgtcacaccc ggcacatcgt
     1561 ccacgatggc cgctcgcgtg ccgagaatgc ggttgaacag cgtcgatttc ccgacattgg
     1621 gccgcccgat gagggcgata atgggcaacc ggccgccctc aagggtgaag agcggggcca
     1681 caggaccgcg cgtttgtttg gtgcgtgaca tggtgcgcga cggtagcata gagatttcac
     1741 cgaacacaat gactcgtctt gctgcgctgg tctccggtat actgccagga tgctatcaaa
     1801 tcgctcagag gccgcccatc agtccgtcga ttggagtcgg atcgacgatg tgttgctcga
     1861 catggacggc acgttactcg accgtcattt cgacaacttt ttcttcgagg aagaactgcc
     1921 gaggcggtat gcggcgaagc atgcgctgac cttcgaggcc gcccgggatc gcttgatggc
     1981 catgtatcgt tccgtcgaag gggaattggc ctggacggat ttacattatt ggtcggagcg
     2041 ggtagacatc gatgtggtgg cgatgcatcg cgaactcgac cacatgatcg ggtttcttcc
     2101 cgatgccgag gagtttctct catccttgcg tcgattgggg aagcgcgtga cgatcctgac
     2161 gaacgcgcat cgggcaggag tggatgtgaa aaccgccaag acggggctgg atcggcatgt
     2221 cgatcgtatc gtggatgcct tcgaggtggg gtggctcaag atgcgatcgg agtattggcc
     2281 gacctgccgg caattggtcg gattcgatcc tgcgcgggcg ctctacatcg atgacgatga
     2341 gcaatgtctc gctgcggcgg aagatttcgg gatcggtcgg atttttcacc gctccaggtc
     2401 gagctctcag gcttcagccg ttccgtccgc gcggtttacc tccatcgaac atttccacgc
     2461 gatcctaccc ggttagcccg tttgtcgcgc atccggtttt tctgcacaat ccatcacgtg
     2521 tcctatttgg cgggatcgag caattcccgg agcttctgcg ccaactcagt ggggagaaag
     2581 ggcttctgaa tgaagccggt gcccggttcc gcgagaaacg tcggtaacac gctcccctcg
     2641 gcatatcccg acataaagag cacccgtagc tcgggccatc gccgacgcag atgctcagcc
     2701 aaggccggtc ctgtcatctg cggcattacg acgtctgtga gcagaatatg aatgggggtg
     2761 cgggcggcgg cgaccaggcg gagcgcttcc tgtccgctgg aggcttccag cagggtgtag
     2821 ccgtaatcgg acagggcctg ggtgagcagc aaccgcaccg cgtgctgatc ctcgacaagc
     2881 aggatggtct cccgcccgca gtgtggacgg gagggaagcg tcggcgtggc cgcaagcggc
     2941 ggcgcttcca ccagtggaaa gtagaggtcg aaggtcgagc cctcacccgg cgtgctgtcg
     3001 gcgaagatga atccctggct ttgtttgacg atgccgtaca ccgtggccaa tccgagtccg
     3061 gttcctttgc cttcttcttt ggtggtgaag aagggttcga aaatatggga gagggtcgtg
     3121 cggtccatgc cctgcccgga gtcgcggatc gacaggtgcg cataggctcc cgggagtaat
     3181 gcgggatggg gcacggggct gtccggtgta atcagcaggg cctgcgtcgc catcgtgagt
     3241 gttccgccgg caggcatggc atccttggca ttcacggcca gattgagcac cacttgatcg
     3301 atctgtcccc tgtccgcgcg aatggtacag ggctgcgagt cgagatttct catcaagctg
     3361 atgtcttcgc cgatcaaccg ttcgatcatg gagctgatcg acgagagcgc ctcattgagg
     3421 tcgaagacct ggaacgtcaa cacttggcgc cggctgaagg ccaggagttg cctggtcaag
     3481 gccgccgccc gctggccggc ggtcaggatc tccgtcagcg aacgacgaag cggatcctcg
     3541 gcccgcacct ggtccaacac gagcgtggcg cacccgttga tgacggtgag caggttgttg
     3601 aaatcgtggg ccacgccgcc ggccaagcgg ccgatccctt ccatcttgtg ggcctgccgg
     3661 agttgatctt cgagttgttt gtgcttcgtg atatcgacga agaatccgat ggagccgacc
     3721 gcctggccgg tcggactctg gagcaggctg ccccacatgt tcacatccac cagggctccg
     3781 tcctttttct ggcggcacag ttccagattg cgcggcgcgg cgccgctcat caccgccgtc
     3841 cacaattctt ccgacacctc ttcctgtccg ggaggcacat aggggagctc cttgcccagc
     3901 acctcgtctt tggtccagcc gaagagggcg gtggcggcgg cattccaggt ggtgacgagg
     3961 cccgcttggt ccaattcgat catcgccagg ggggactctt cgatgatggt ctgaagatgg
     4021 gcggtcgtcc ggcgcaactc tccggtgcgt tcacggacat gttgctccag cgcctcttgg
     4081 gcttgacgac ggtccgacgc ctcaagagtc agggtcgcca tggcggccag ggaggccgcg
     4141 aacgtctgtt cctctgtggt ccagactctg gccggaccga tgtgttcgag gcataacacg
     4201 ccgactacgc gaccatgcct gcggatcggg gcgtccaaca tggccacgat accaagcggg
     4261 gtgaggtacg acggagtcaa ttcactcgtg cggggatcgg tctgcgcgtc atgggccgcg
     4321 agcgagtagg gttcggtttc gagcgcatgc aggtaggccg ggtattcacc ggtcgagagc
     4381 gtggttccct gtagatggtg tttgggagtg gcttcgaaca gatcctgcag gatcaatgcg
     4441 gactgatgct cgccaaagag ccagatgccg gctcgttcga cggcaaacgt gttcaccgca
     4501 tgttcggtca tggccgggaa ggcttgctgc gggtgtccgc tatggatggc cggatcttcc
     4561 gccaattcat gcagcacggc ttgttgtgtt tctaatttcg ccaagcgcag ccgttcggcc
     4621 tcttcgcgtt gtttccggtc cgtgatgtct tccgaaatac ccagtagata gcgcggcatg
     4681 ccggcctggt cgtagagggg gagcttcttg gtgtggagcc accggagtcc atgatctttg
     4741 gtctgaatcg gttcggccga aatgtccatc atcgtgccgc cggtcaggac ctcgcggtct
     4801 ctggcggtaa agaattccgc ctgctcaaga gggaagaaat cgaagtcgtt tttccccagc
     4861 agcgcctctc gcggcaggcc gatcagttct tctcccgctt gattgaactg gacgaagcgc
     4921 aattcttgag cgtcttttaa aaagaccatg tggggaatgt gctcgacgat ggactccagc
     4981 agttggcgcg tctgggtcag ggcatcttcg atgcctttct gttcggtcac gttctcgcac
     5041 acgatgatga tatcgaactg tccgtcggag ttgcgcacgg cccgggcggc ttctctcacc
     5101 cagagcaggc tcccgtcctt gcgcatcttc cggaaattcc agcggaggac ttctcccggt
     5161 cgacggagac agtcgacaaa atgttgctgc atgctgccgt gatcatccgg gtgaacgatt
     5221 cccgccactg gttgaccgat cagctcgtcg gcctcgtagc ccaattcggc ggcggtggtg
     5281 gtattgaccg tcagcaatgt cccccgggcg tcgaccgtca gcagcatctc agggctactc
     5341 tcgaacagcg cacggtatcg ctcctggctg acgcgtaacg cttcttcggt ctgtttccgg
     5401 acggaaatat cgcgtaccac ggcctggaac ccgataacct cctggtcgcg ttgcagcagc
     5461 tgcacatttt gtccgaccca cagggtctgt tgcgtgtggg tcatcagggg aaattcacaa
     5521 taggtgctgg ccgtcttccg gagaaattgc cggaggtaga agcgttccgt cgggcggcgg
     5581 tagtcgggat gaacgaaatc cagcgcacga tggccgagca tctccgtctc gtgatacccc
     5641 aggagaagga tgaccgcggg attgatgaac gtaaaacggc cgctccggtc tgtccgatag
     5701 atgatgtcct gggcatactc catgatgaag cgatggtgtt cggtcttgac ggtgaggagc
     5761 agttccatct gctccccttc ctgcagttgc ccggtgagtt gatccaccag ttgggcgttg
     5821 tgatatttca gccggagggt ttccaggacg gtgttggacg tccggtacgc cgtgtagacc
     5881 atgagcagcg agaagagcat ggtgcagatc gccatcggca gggcttgctc atggtcgagg
     5941 gcgaagagtc gcaggatgag tggcgtcaac atcggaagag agaacgacag aaaggcgtcg
     6001 aagcgagcgg ctagggtgga agcggcgccg gctgtaatgc ctgcgagcac gaaggccaga
     6061 aagacctggt gggccggtga ggattccgga aacagcaaca gggctgaggc gccccagccg
     6121 agacccgcga gtgtcgtgcc gaccagaaat gcccgctccc agcgttcgat gttggcaggg
     6181 cccggcttgt tggccgtgaa cgatcgggcg agcccgtatc ggcctgcggc gaggatcaga
     6241 tggtacacca gccagatgag cagccggtcg tgcgggacga catgccaatt gaccgcgatc
     6301 agggcgagtg cgcagccgag tccggccacc agcgcggccg gcatcgcctc ataaaggagc
     6361 gtcacccgtt gcaaggcgat gctgctgtcc cgggctgaat ctatgggttt gaataatgga
     6421 gaggtcgacg gaggctctct gtcggggaac gactgcgagg aggacatccg ggtcactccc
     6481 gtctgctgca tggacgaatg cgacgatcgt gcattatacg gtgcagtcta caaaattgcg
     6541 gatagggata caatttccct gtttcatgtc gcgcacggtt cggcgaggcg aaggtcgtga
     6601 cgactgggtc aggtttcgag gctggggtgt aggggcggtt gtccgttgac gcgggcggtg
     6661 ccgcgataca tttccccgcc gattctcggg acgccgggaa ggcaataacg atcaagctgt
     6721 tccagccgca ggcctgcgtc cagtactagt cgatcgatgc ggcgattgag attgcagccg
     6781 caggccagga ggtgttgcag cgggttgagt cgatcctgcc accgggcgat gaggggatcg
     6841 tcgctgcggc catgctccag gaacaggaag tggccatccg gcttcagcac ccggcgaatg
     6901 tcgcgtagcg ccctcgccgg atccggaatc gtgcagaggg tgaaggtgct gaccgcgtag
     6961 tcgaagctgg catcgtcata gggcaatcgt tcggcactga catgctgaat gtgaacgggg
     7021 aacgaagcct gtgccacccg cacggcgact cgatccggca agagtggggc gggatcgacg
     7081 gtgtgcagag cagtcacggt cgtggggtag tgcggcaggt tcaatccggt tccgaacccg
     7141 atttccagga cgactccatg taccggcgtc agcaggagcc ggcgttcggt ctgaaaccgt
     7201 tcgccccgga gcacccagtc catgagtcgc ggaaagatgt aggtagcgta catcggcgtt
     7261 ggcaaaagtc tgtgaggtcg tgcgcggtcg atcttcggcg attggttgtc tcggcatttg
     7321 aacaggactc gattccggga tcaagggagg ggggatcctt cgcggtcgaa tcggtccctg
     7381 tttgtgccgc ctcgtccttg tcgctccggg cggcgtgtgc tagagtcgtc cgttctttca
     7441 ttcaacagga ctggttgccc tgcgtatgac gataccacaa tgagcaagac gtacgatcat
     7501 caggccctcg aattgaagtg gcagacctat tgggagacgc atcgcccgtt tcgggcggtg
     7561 gacgatcatt ccaagcccaa gttctattgc ctcgatatgt tcccataccc gtccgggtcg
     7621 ggtctgcacg tcgggcactt ggaagggtat accgctacgg atatcgtatc ccgctataaa
     7681 cggatgcgcg gcttcaacgt gttgcacccc atggggtggg atgcgttcgg gttgcccgcg
     7741 gaacagtatg cggtgaaaac cggcgtgcat cctgccatca ccactgcgca gaacattgcg
     7801 acctttaaac ggcaaatgaa gcgagtcggg ctctcctacg attgggaacg cgagctcagc
     7861 acgaccgatc aggactacta tcgttggacc cagtggatct ttctgaagct gttcgaacgg
     7921 ggccttgcct acgtggcgga agtgccggtg aactggtgtc ctgccttggg gaccgtgctg
     7981 gccaacgaag aaatcgtgga cggcaaaagc gaggtcggcg ggttcgacgt cattcgcaag
     8041 ccgatgcggc agtgggtctt gaagatcacc gcctatgcgg agcgattgct ggaggacctg
     8101 accctggtcg aatggccgac cagcacgctc gagatgcaga agaattggat cggccggtcg
     8161 atcggcgcgg aagtcgattt tcccttggcc gacaagacgg gcaatttgcg cgtgtttacg
     8221 acccgccccg acacgctctt cggtgcgacc tatatggtat tggcgccgga acatccgctc
     8281 gtcgacgtgg tgacgacgcc ggagcagcgt gagcaggtga cggcctaccg cgatgcggcg
     8341 tcccgcaaga gcgatctaca gcgtcaggaa ctggacaagg tcaagaccgg tgtctttgcc
     8401 ggcgggtatg ccgtcaatcc ggtgaaccag aagcgcctgc cgatctggat cgccgactat
     8461 gtcctcatga gttacggcac cggcgcgatt atggcggtgc cggcacatga cgagcgtgac
     8521 tgggccttcg cgacgcaata ccacctgccg atccgagagg tgattgaggg tgggcaggtc
     8581 gataaggcgg cgttcgtcga gacggatcgg ggacgtgtca tcaactcgac cacgcccgat
     8641 ggatcctgct ctctcgatgg actattgccc agcgaagcga ttccaaaaat gaccgcctgg
     8701 ctcgaaactc agggcagggg taagaagacg atcaattaca aactccgtga ctggctcttt
     8761 gcccggcagc gttattgggg cgnnnnnnnn nncaattgtg tgggtcgacg gcgagccgcg
     8821 tcccttgcct gaggagcagt tgccgctccc attgcctgag accaagaatt tcaaaccctc
     8881 cggcagcggc gaaagcccct tggcgaatct ccacgactgg ctggagacga cggatcctgt
     8941 caccggcaaa ccggcgcgac gggagacgaa tactatgccg cagtgggccg ggtcctgctg
     9001 gtattacttg cggttcatcg atccgaagaa tgccatgcag atggtcgatc cggcgaagga
     9061 gcgctactgg atgccggtgg acctctacat cggcggcagc gaacatgcgg tgctgcatct
     9121 attgtatagc cggttctggc acaaggtctt gttcgatgcc ggggtggtca gtacgccgga
     9181 accgttcaag aagctggtgc atcagggcat tgtgttgggc gaagacaatc agaagatgtc
     9241 caagtcgcgc ggcaatgtgg tgaacccgga cgagatgatc gaccagttcg gggccgacgc
     9301 ggtacggctc tatgaaatgt tcatgggccc gctcgaatcc atgaagcctt ggagtacccg
     9361 cggcgtggaa gggatcacac gattcctcga tcgggtctgg cggttgatga tcggcgacga
     9421 gggcgcattg agtccggcgg ttacggatgc cgagccggcg cctgaacagc tgcggctgct
     9481 ccattgcacc attaagaaag tcaccgacga tatcgacgcc ttacgcttca atacggcgat
     9541 ctcgcagatg atggtgttca ccaacgagat gacgaaactg gagcagcggc cgcgccgcct
     9601 gctggaaccg tttgtgttgt tgctcttgcc gtttgcgccg catctgagtg aggagttgtg
     9661 ggaacgtctc ggtcaacaac cgagcgccgg ccagcagcca tggccgcagt tcgatcccgc
     9721 gttggtggtc agcgaccggc tgacgatccc gattcaggtg aacggaaagc tccggggaaa
     9781 actcgaggtc gatgccgggg cttcacgcga gcagttagag cctctggcta agaaagaagt
     9841 ggctgaatgg ctgcagggga aagagccgaa gaaggtcatt tacgtcgaga agaaattgat
     9901 caacttcgtg gtgtgatgcc gtgcgccagc ctgaacgtca caggaacaac gcagcacgtg
     9961 ccggcccgga ggttcctggt cgcgtggtgt ggctgattat tttctttgcg ttgagtgtct
    10021 cgctccccgg ctgtggctat cagtttcgtg tgcagggcgc cggtccaacg gtcggcggtg
    10081 cgccggaggc ggcgacgaag cggccgcatg cgccacgcct ggccatcctt cctattgcca
    10141 acagtaccta cgaggcgaac ttcgaggtca agctcgccaa ttacctgcgt cgggagttct
    10201 cggctggggc cggggcggaa atcgtgggcc cttcggccgg ggccgatctc tatttgtccg
    10261 gacagatcat gcagatcacg ttgccgacct tgagtttcga ccagaccacg acatttgaga
    10321 gccgggtgga aatgctcgtg agcgtgagga tcgaagaggc caaaacgaaa aaggtcgtct
    10381 ggtaccaggt cgcgaaggga acctctgagt tttttctgac ccaggatctg caattcaacc
    10441 gggtgctgca aaaccgggcg ttggagcaag caggccggtt cgtcgcggaa gacctggcct
    10501 cacgattcct gctctttctg gatacgggcg agttggacaa ggcactgaag cgtccggcga
    10561 aagcggaggt cgctccggcc ggcgggacac cgccttatgg ggcccgataa ctgatggaga
    10621 gtttctgcgg atgagcgctt cagtgatgag tctggaattg ccccaatccc tcgcgcgcag
    10681 cggggtgatg ccgctgtatg ccgtggtcgg cgaagaagac tatcttcggg accagtccgt
    10741 ggccgcgttg cgcgccgcag ctttgggccc ggcggcggat accggattca attacgatat
    10801 ttttcacggc gacgactgct cggttgagga tgtgttggcc tgcgcggccg aaattccggt
    10861 gttcgccgaa cgccgcgtgg tggtctataa gtcggtggaa aaactcccgg cccgagaagg
    10921 ggaaaaactc ctctcctatt tctccgctcc gaatgacaca acgacgttga tcgtggtggg
    10981 tgtcaagctg gatggacgga tgaaatggac ccaagccttg acgaaagggg cagtgacggt
    11041 caattgcgcc ccgctgcgcg atgcgcagct gtcggcgtgg attcgccagg aagcgacggc
    11101 gctgggtgtg cgcatccacg aggaggcgac gcaattgctc aaggaggtgg ggagcgagtc
    11161 gctctacgcg gtgaagcggg agttagagaa gctcgccgcc tatgtgccgc cagaccgaac
    11221 cgtgcaaccg gcggatgtcg aggcgttgag agggacggag tccggtgcat ccgtcttcga
    11281 tctcatgaac gccatcggtg cgcacgatca tggacgggcg ttacggatcc ttgcgcgaaa
    11341 tcttgagaat ggagaggctc cgctccggat tctcggtgcc ttggtctggc aataccgacg
    11401 gctgtggaaa atgaaagagc agctcaaagc cggcggtcga gaaggggaag cggccaggac
    11461 gttacggatg gacccatctc gggtgcgcgg gtttctggcg caattctcag atgtgcaact
    11521 gacgcacacg tttcgctggt ttttggaaac cgactcgaag ctgaaaggcg gaagtggaag
    11581 cgcccctgtt cgggtgatgg aagacctctt gttccgactc tgtggccagc cgtcgaaatc
    11641 agcatcatcc gcaacgcggg tgcagccatc accaccggtc ccgcgcccat caggttcgag
    11701 accggtgtcg aatgtcagaa ccgttacgcg gaagcggtga ttgcgttcac acgcacggtg
    11761 agccgggaga ctcggcgcga ggccgtgttg ggcttcaaga cacctttggt gaccgctttg
    11821 ctcaacgctg aagtggcttc ccgcagggtg gccttggctt catccggctt cttggccgcg
    11881 atcgcatcct gaaccttgcg gaggatgctc cggaccgagc tgatggtgga gcggttgcgc
    11941 aaacgacgct tctcagattg acgggcacgg cgaatcgtgg atttgtggac aacaggcata
    12001 cggattctcc tacgttcaaa gcttgagctt gtagcatagg atgagaagag aggtcaagga
    12061 cactgaaagg agtcagccgg gccccgaggc aaccaggggg taacggcgta cacatgatga
    12121 cacggattga tgcacgagat cggttgattg tcgcgctgga tgttccctcc gcggcggaag
    12181 cggatgcgct ggtggctcgc atgggagacc aggtgcgatt tgtgaaagtc ggcctggagt
    12241 tgtacacggt ggcggggccg gacatcgtcc ggaagctggt ggaccgtggg aagcgggtgt
    12301 tccttgatct caagttcctc gatattgaag aaaccgtccg gcgtgcgacg gctcgggtcg
    12361 ccgctatggg tgccaccttt ctgaccgtcc atgcgaatcg aaaagcgttg attgcggcgg
    12421 tgcagggacg gggatcgtcc gatctcaaac tgctggcggt gacggtgctg accaatttcg
    12481 acggggaaga tttgcgggag atgggcattc agcgaagcgt gcaggatttg gtaacggcgc
    12541 gggccatgct cgcggccgaa gtgggctgcg acggcgtggt ggcgtcggga gaagagccgc
    12601 aggcgattcg tgccaaggtg ggaccgaatc tggttatcgt gacccccggg gtcaggccgg
    12661 cgggaaaagg gacggatgat catgcgcgag tgacgacgcc gacccagact attgcagccg
    12721 gggccgacta tctggtgatc ggtcgtccta ttcgcgatgc tcaggatcct cctgccgcca
    12781 cggcggccat cctggaagaa atgcaagcgg ccttcgatcg ccgggggcgt tgacggtcaa
    12841 gtcccaagtt gatttcgtcc atgcgaccgg ggatacagaa tgcgtcgcga cttcgcccgg
    12901 ccactctacg cagcaaaagc tgagtcaggg tgaggtctcg gggcggttgc gactgttctt
    12961 cggggtttgt taagatgcgc gttcctcgtt gaccttgtgt ggtgggtgta gctcagctgg
    13021 ttagagcgcc ggattgtgga tccggaggtc gcgggttcga aacccgtcac tcaccccaac
    13081 gtctgcggat acggatcact cgagcgggta gccaaatgac cggtggcaac aaacaggtcg
    13141 ctccattggc cccgcgttcc tcgccggctc cggtcgccac gacatttcag ttgcggtcct
    13201 ttcgtcgttt tcccgttcag tgttctgtgt attactccag cgatgcgctc cagggaaccg
    13261 gcgtcgcctg gaacctctcc ctcaatgggt ggcgtatcga cgggacacat ccggtcgagc
    13321 ccggaacggt cgtgaccctc tgcgtgtttc ttcccgacca gcatcccacc gtgtttatag
    13381 accgcgcagt cgtgcggtgg tcgcgtgggc aggaattcgg gattgaggtg aatgagatca
    13441 aggccgatga gcatgctcgc ctcgaacagt tcgtcacggc cttagtctga ccgctgccct
    13501 tctccatttg tctctttccg atctatgaga ttgcgaagcg ggactgcctg ccctgatgag
    13561 aaactgaata ttcttggcca tccccagcga gttgagtaga ctgagtcgtc gcgaggctga
    13621 gacggcatcg agccctctcc ctcgccggaa atcatggcta agtcacagac atctcgttcc
    13681 cattcccgtg tgcccgtcag ctgctttctc tattatcttg gggagggcct ggtcggaacc
    13741 ggcaaggtgt gcgatctttc ggtcaagggc tggcggatcg acggggataa gccggtcacg
    13801 gtcggcatga aactcacgtt gcgcgtcttt ttgcctgatc agccgaaggc gattgatgtg
    13861 gatggggtga cggttcagtg ggtgcaaggc cgtgtctttg ggcttgaaac cgtcaggatg
    13921 agcgctaccg cccaagcacg catccacaaa tttgtggagt cagtgctcaa ggagtcggga
    13981 tcttcacgag ttgcgtgact ccttaatcgt ttatcggtta ttcggctctc cgacctagtc
    14041 tgggtccggt tcgcagcagc agctctcgtt tccgttccct cttccttccg tttcgccggc
    14101 gatactacga acctctctga gcaggataaa gacgatcggg ccggagagcc cccacctttc
    14161 ggggactatc aggctattcg ttgtttttca tattctgccg aggcgtttgc ttggaggaat
    14221 ggatggaaca acagaatcgc ccgcgtttta tcgtcgagtg taccggatca cgctccgaag
    14281 atggtttgtt ggtgtggaac ggccggatgc tcgatctctc gattccggga tggtcacgga
    14341 ctggactcaa agggttgcaa gcgggagact tactccaact gcacttgcat attccggggc
    14401 agcccaggcc cctctcggtg aggctggcga cgatccgctg ggccagcgag tcgaagctgg
    14461 gggtcgatcc cattcttatg gatgccgatg accaactccg tttgaacgac ttcgtcagcg
    14521 cccatgacac cactccaccg ttcagtacgg atcggaaaga acagatcgtc atttccgatg
    14581 ccggatagcc gattcgaatg ttccttcgct tgtcctcgct tttcttcctt acccgccggc
    14641 tcgtgattct gcgtacatgc gccttgtgtc attgaggtct gccgcctatc gggctgctgt
    14701 gttggtgaaa tgtgtgagcg agatgcggca ggtagtcaag cggcctggcg gcaggattgc
    14761 ttgacatcat cgggcaagat ggtgtctctt agcgaagaca cgttgttaca cacaccagcc
    14821 tttcgccggt ccagttcggt catgtcatag atggtttgcc agggcggagg cgtgcgagac
    14881 ttgacgtatg acgcctggcc cccaacttcc cgatgcgacc cataccaccc cactgacgcc
    14941 tgaaacggag gcgcaggtcg accaattcgc ccatacggat attttggtcg ggattcccag
    15001 cttcaacaat gtcgaaacca tcgggcatgt cgtcaaggcg gtgagcgccg gcctggccaa
    15061 gtattttccg gaagcccgcg ccgtactggt gaattcggac ggcggctcga ccgacgggac
    15121 gcagcaggtg gtttcgcagg ccgtcgtcga tctcaagacc ctcttcatcg gcgatcagca
    15181 gagctcgctc cacaagatca tcacgccgta tcacggcatt cccggtaagg ggagcgcctt
    15241 tcgcacgatt tttgaaattg cgcgtcgcct gaaagccaag gcctgtgccg tcgtggactc
    15301 ggatctgcgg agcatcaccc cggaatggat tgagttgctg gtgagcccgg tgtttgagca
    15361 ggggttcgac tacgtcgcgc cctattatct gcggcacaag tatgacggga ctatcaccaa
    15421 cagcattgtg tatccgctga cgcgaaccct ctatggtcat cgtatcaggc agcccatcgg
    15481 cggcgatttc ggtttttccg gccagttggc ccagcactat ctcgacaagc atgtgtggga
    15541 atcggaagtg gccaagttcg gcatcgacat ctggatgacg acggaagcga tcgccagcgg
    15601 agcgcgcgtc tgtcaaagtt ttctgggcgc gaagatccac aaccccaaag atccggcggc
    15661 ggacctgtcg gcgatgttgg tgcaggtgct gggcgctgtg ttcgcgctga tggaagatca
    15721 ctatgcggtg tggggcaaga cggatggctc gaatgccgta ccgttgttcg ggtttcaata
    15781 cgaggtcggg gtcgagccgg tcaacgtcaa tgtcgatcgg atgatcagca cctttcggca
    15841 gggcctgagt gatctgggga cgatttggga tcagattctc gctcccggca ccagacagag
    15901 tctgaggccg ttgggaacct gttcggcgca agactttcgc attgccgaca atctgtgggc
    15961 acgggtcgtg tatgacgccg ccgtggccta ccgtaatcgc gtgttgccgc gcgaccacgt
    16021 cttgaaggcc ttcactcccc tgtaccttgg gcgaaccgct tcgtttgtgt tggataccca
    16081 ggggctcaca tcgagcgagg ccgagggccg catcgaagcc ttgtgccagg cgtttgaaaa
    16141 ggataagtcc tatctggtgg cgcgctggaa tgagccacat gcagggtaat gcgcgcaact
    16201 cgatccatct gaaggcaggc tgcagtagag gccggaggag ggaagtatga aagaattttt
    16261 tgagaagggg ctactcgatc ccctggagtt gatggcgcgg caggtgcttg cggtgttacc
    16321 gagcctgttg gcgatgtcga tcattttctt cgcgggattg gtggtcgcgt ggaccgccag
    16381 tcaggcgctg gagcggttgc tgcgggtggt cgggctggat cgattgttcg atcgactggg
    16441 agtgacgggc gcattgctgc gcggaggggt caaaaccgat ccgtcacgtc tggtggggca
    16501 agcggcctat tggttggtca tgattttcgc gaccgtcgcc gccctgggtg cgctcaatct
    16561 ccagccgatc aacgatttcg ccaagtcgtt cctggcgtat gtgccgcatc tcgtgacggc
    16621 gggtctcatt ctcgtcgccg gttggttatt atccaacttt gtgtcccagg cggttctgat
    16681 cgccgccgtc aatgcagggt tgcctccggc acgactggtg gcgacctgct cgcgatgggg
    16741 gattcaactg ttggcggtcg ccatggcgct tgagcaactc gggatcgccc agaatatcgt
    16801 ggtggtggga ttcggcatta cactcggtgg gctcgtcatg gccggggcga ttgcattcgg
    16861 cctgggcgcc aaggatttgg ccaaagacta tcttgagcgc gggctttcga ttcgcacgcg
    16921 agacggggcg cctgatgact tgcgccatct ctaaggcaac cgctgtttcg acacctaaca
    16981 tatagctggt aaggatctag cgtgaccgat aaaatttcag atgtcgatgc tgaggccaag
    17041 gcgcggcgct ggctgggcgg cgaagcggcc gtgccccgtt acccgcaccg gcgccagagc
    17101 ccgttcgtgt tcaaccgctc gcttgtgggc cttgtggcgt ttgcggtcgg actagggttt
    17161 gccggtggac ttctggcagg tctccagtgt cggcgtcgtc ctcctgtctc tgcgaaggag
    17221 taaccatgag tcgaccattg cagcgttggt gccgggtggt tcgggagtgc cgatccgctg
    17281 atcgtcaaag ggtcggcaac agatccatga ggcttcggtt ccatccgaca ggccccaccc
    17341 cttcggcgcg ggtgaggtgg ggaaactgca cgtccggatc gtaggaaccg tccggcttcc
    17401 ggacgaggat ggggtaggtc actgcttcca gcatgggaag gtcgttcaga ctatccccaa
    17461 tgcccacggt gatcagggcg cgtccttcct gctgtgcgcg ccgctcgtac caggcaatga
    17521 gtcgccggct cgcaacccct ttatcgtttc ctcccatcaa atgatagaac cggcctcctc
    17581 gggtccagcg caagccgcgt gttgccgccg ctgctgacaa gcggttccag accattccat
    17641 tcgattccac aacaaccggc tcatcgtatt cccgttgggt ggccagcaga gtctccgcag
    17701 gagacagccc cgtgaggtct gacacctctt cgagagagag atccccgaac cctcgcaagc
    17761 gacagcccag atcccgcccg atttctttca gggccgtcct gagccggaca tagggggtgc
    17821 cgatttcgac cacctcgtag tccccgcggg gtgcggcctg ctccaaagga aatgggaaat
    17881 agcccatggg gatgaacaac gctccgccgt tttcgacgat aaagggatgg cgattgtcca
    17941 ggcgaagtcg cagcggctcc atttccgatc gggtcttgct cgaaaccagg ataagcgttg
    18001 cacccagggt ctgaatggca gagagggcgc ccgtggctgc ctcgtaggaa taggtggtgg
    18061 cgtcgagcag ggttccgtcg agatccgtaa ggatgaggat gtgggccttg gtcaaggagt
    18121 cctccttgat cacagtatac gaacccccgg agacggataa cacatcattc tcgggcagtc
    18181 agtgctcccg tctgccgacg gtggcgactt ccagactgtg tgtcagaacc ggcacaggag
    18241 aatccctaat caagatcgtg cccggtaatg ccgaaaagga tagacacatg gctgtgtcgg
    18301 agagctgggt gacgtcatcc aggtaaacgg caggcattcc acggtcatca ccgcatcatg
    18361 gctcagaaga ggaccatgta gtggccaagg cacttccctt gctttccccc gaatggtgca
    18421 gttgttcaag gcggtggatt gcccggttgg gatagtccct gctggcattc tagtcatgaa
    18481 gtcatgcggc gcgagaaatg tctgtaactt tataggttta tacagtgcga tctgataaga
    18541 aatctcctct caagtcagac aaaaaagtcc ctccgaaggt ccagaaaaag gggagcatcg
    18601 gctttaacaa aaagtcggcg cttctcgaag atgccattac acaaatgaac gccggcaagt
    18661 acggccgggc gtcctccgcg ttgaaagacc tgctcgcgct tgacccgctc aacgcggaag
    18721 cccgacgcct cttcgccact ctccatctgc ggctgggcag tctcatgagc gccaggacgg
    18781 cgttcgaatc gctggctcgg gaagcgatgg agcggcagga ctattggttg gcggagtcgc
    18841 tcctgcggga atatctgacg gcgggacccc gctatgttcc tttcctggag atgctgggcc
    18901 gtgtctatga ggaaaagggc gacgtcatgg cggcggtggc tgagtacggc aaggcggtgg
    18961 aggtgttgct ggaagatccg gacaccgacc atccgaaccg ggccagcgat ctctttgtgc
    19021 ggattcgttn nnnnnnnnnn nnnnnnnnnn nncctagccc caggaagtcc ggtggccttt
    19081 cgtttcgcgg ccatgttcga ctctgtaacc gggcaggtcc tgcaggttac tccccagcct
    19141 cctgtggtcg attctatcaa cgagaccgct cacgctccgg cactgatcga gactccccct
    19201 ggtacagact ctgcggtggt catgccttgg gagcacatgg aggcggcctc cagtgatgtc
    19261 ccacgccaac atgagattca ggtctcggcg gtcgagcagt cccctgaggt gatcgctttt
    19321 accaccacct cctcgacgct ggaggcggtg acagaagatc tgctgcaggc caagccagag
    19381 gagtcactgt cggttacgct gcccgtcgat tcgccacagg agtcggaggt tcaacctcaa
    19441 tctgagtctt cagtttcagc cgttacctcg gcggtggagc cggtgcaggc tgccggtgaa
    19501 agtgatcctc ctgtcttaga ggtcgatcct gcttcgatag agccggccga aatagcttct
    19561 gcatcggttg ccgaggtgtc cacccccacg atggacgcgt cggcgaaaac cggtcccgac
    19621 tcgatcgcta cactgtcggc acactccgcg ccgatgccct gggaccaagt ggaagaggcc
    19681 gctgggatca ttcctccgca gccagaggga ccggcggcga cgccggcgga cattgcgcct
    19741 cccccttcgg gagaggtggg cctcacgcca tcagtggccg agtcgccagc tccgtcgatt
    19801 gaggtcctca cgcagcccag tgggcacccg attgccacag caccggcgag ccctgctccc
    19861 atgccttggg accagatcga agaggttgcg gtcgtaatcc cctcgccacc gtcgagtccg
    19921 gaaccggtca ttgttgacgc cgagtcattg ccgccaacgc agagcgaggt ctcaacgctg
    19981 ccgctgaccg aattagctcc attccaacct gtcgaacctg aaccgccggc tgccgccgct
    20041 ccgatgccgt ttgaagagat ggacctccag gcgccagtgt ctgagcccat cgcgccgacg
    20101 atcgaagtgc tcacgcaggc cagtagccat cctattgccc aagcgccgac aagtcctgcg
    20161 ccgatgccct gggatcagat cgaagaagca acgggtgctg tggatccgcg cactgagacc
    20221 ctgatggagc ctggaacgga cgaggttgag aaggcgatcg agtctcctgc gccaaaccag
    20281 gcggtcgagg tgagtcaggc cgcggagtcg gttccttcat cgcgtccttc ggaactcacc
    20341 tcatcgggac tctcatggga ggaaattctt gcggctgtgg cggccatgca aacgtctcct
    20401 gcgcccgctc agacgctttc ggatggtgcg cagaagcctg cgtctgatgc tgcggtacag
    20461 gagcagacgg ctggcgctct cacggctccg ccagctcctc cagaggaaga gtcacccttg
    20521 ttcgaggcga cggtcccgga catcccggct gatcaggccg gtgcagccac aaccttgtcg
    20581 gcgcccatgc catgggaaca gattgaagtg gacgatgtca cgattccgcg ccaggagcct
    20641 gagccagaat ttggcccggc ctttgccgag ggtgccgtgg cagtacagga ctccacggtg
    20701 gcgttgcctg cagcgccaga cgtgactgag gcaacggttg ctggagaggc ttgtttggct
    20761 gagaccgtcg ctgatcgccc tgtggagcct attgctacag tgaccgctga attgcggatt
    20821 ctctccccgg atgctccgac caaggcgcct gaggattcta ttcgtattcc agtcgagccc
    20881 tacaaggcgg tcgaggcgcc gactccggac accgtgcctg aagctgaaca accggccgaa
    20941 tccacgatcg atcgagcggt ggaagctccg ttgcgtctgg ccgacagtga gtcggttgtc
    21001 gccacgagtt tagaggaacc acctcctgct tcagtcgaac cggtgacgga gtttgccatt
    21061 gccgtcagca ccgacgtgac gccgatggaa ccggagcagg tcaccgtcgt agagcctcag
    21121 gccccggtcg gcgaggcagt tgcggaactg ccttcggaac cagtcctacc ggtggagcag
    21181 cctgcggaga tgctttgcgc acaggaggtc atggtcgagc ctgcgccaga ggtggttacg
    21241 gagagccttg tctctgctgc cgcgccgtcg ctagccgagg agaagccggt tgctgaagcc
    21301 gagccggttg caccgttgtc gccggcggct ctggaagtga cacctgaacc agagccggaa
    21361 atggtgttgg tcgagcagga gcaatcctcg acgccagcgg ctgaggttcg cgacgttgat
    21421 accgttgggc t
//
LOCUS       DAORQN010000016        20926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_172_length_20926_cov_12.024100, whole genome shotgun sequence.
ACCESSION   DAORQN010000016 DAORQN010000000
VERSION     DAORQN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20926
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_20926_cov_12.024100"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1346)
                     /locus_tag="PK614_02350"
     CDS             complement(21..1346)
                     /locus_tag="PK614_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81082.1"
                     /translation="MTVQLIDYAMMAGASYISTRPDSNKFPIPMGWATLPSPNGYRLD
                     PSSGFEAVACQRGNEIVISYAGTNPSDFTGDIAADIGLATGIGSVQLEQAAEYYLQVK
                     AANPNATITFTGHSLGGGLAALMGVFFGKQTVTFDQAPFANSAELSLIAPDVAANLRS
                     YLVNQLDLSGGRKYSDDALQGLTDFLNVRAALPMGEIPNTSLVKNINVDGEFLSGVPW
                     NIQDRIGQSSHIPIHAPSVSGDDLHSVALLTAFLQSMQSAQSGQALNDVTFKLTNLVE
                     MIFDDNLYAHPTTDADNRNFLERLVQNEQGNAMVTRFTEDLWKLAQDGGLTMADGPTP
                     ATNLVSKALIAFAMQMYYEDTVNATNANKELFTPVTGGLQFDRADVAASLEQAKGCTD
                     FQYYLVNAFSAGDRQVIQSLLPTLRDWHVQGGDVANDASYRNSFERSVA"
     gene            complement(1404..2045)
                     /locus_tag="PK614_02355"
     CDS             complement(1404..2045)
                     /locus_tag="PK614_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81083.1"
                     /translation="MRARWCALPFGGDSWKEEVLLHDGSKLVITRSQTYGGRHEIGQP
                     SPIKEHTVTFTLPHTNKTMTWTSEYGKELGRTNFHLLAVHVLNGTPYIVAEPNLCLSY
                     NKWGRPNPPYVFFKYDGTTWQRIPLEDLPTEFTTMNVVLGLSRQFVDAMVKQGVVSVE
                     HIQKQNSQLIQPEYKAILREPVKPGSEASAVNCLEMVFHKGVWVGGSGGSIGK"
     gene            complement(2360..3073)
                     /locus_tag="PK614_02360"
     CDS             complement(2360..3073)
                     /locus_tag="PK614_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB81084.1"
                     /translation="MNGAALSRLGVNGDIVQEEQKASVSGQHEVKILIVHPDRLFCDV
                     LTMALAVSPYVVIVMACVADAEELVQQETVDPDVVLLGGQAGSRETYGQALRVHTRFP
                     AARILLIGRPLVTMRSRRTQDQGWSASIPMVLGDSSFQGLLQVVHRLSRLHLSRSSDP
                     QTADPSLVKSAASWDDGLDQRRFGLTPREVEILRLRRNGLNNKDIAVALRIGTQTVKN
                     HVRNLSIKIGLHRVRGGQR"
     gene            complement(3100..4347)
                     /locus_tag="PK614_02365"
     CDS             complement(3100..4347)
                     /locus_tag="PK614_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB81085.1"
                     /translation="MTTLPVQEIHLSLRAAMAAAAQQNPSVLLSKERIEAAKGEVTTQ
                     LGAMLPNLSANVRQSQQTQFLGTFGLAPVRTEPFSIFDARVSASQNLFSLSLIQRWRA
                     SREALQVAEFDAQSSRFDTMASTGLAYLEGVKAATALAMRQANQQLIRELLATAKVRQ
                     HEGAATGLEVARLEGQLANEQQQVVAAQADLERAKNSLGNLLGLTFEVRMTLTDPLQP
                     QVPEAAAAQAAWEQAVTNRAEVQAQAKRIRSAELSYSSVTGERLPSLVAQGDTGLIGN
                     RWNNSLDTYNMALLLQIPLFDGGQREGRISTARSQVRQEAFRMQVVLNQVRAEVNEAR
                     IALAAARDKAALAQAGLQAATKETALARERYAVLTAASQFDVITAITSMARARENLVG
                     ALFELNAARVNYARATGTLESLS"
     gene            complement(4455..5858)
                     /locus_tag="PK614_02370"
     CDS             complement(4455..5858)
                     /locus_tag="PK614_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRB81086.1"
                     /translation="MKRTSMMRWWQVWSAAWEAERRQPVAATPSRRSVEFLPAALEIQ
                     DAPPSPIGRAMLWTIMALFTVSVVWASVGRIDIVATAQGKIIPNGYSKIIQPYESGVI
                     TAIRVQDGQVVKQGDVLIELDPTQNQADRDRAFNEYRAAKVEAARLQALIAGVPTLVV
                     PLKSDPGYVRLQQQLLHDQLAEYQARVDAAQHIITQRRAAIDATRENILRLEATVPME
                     TERAGAFKRLMERDAVTKLDYLQAEGQRIDKLQELAGQQHKLRQDQAALAEAEKNRRA
                     LVSEFQQTKQAELSALETKAASLSQEVTKAGQKAELQRLVSPIDGVVQQLAVHTVGGV
                     VTPAQPLLIVVPQDHPVEVEAQLENKDVGFVKEGQSAEIKVETFPFTLYGTIPGTVLT
                     VSDDAVPVNKDKPADGLVFATRVSVARGTIPVEGKLVHLSPGMAVTVEIKTGRRRVIE
                     YLLSPVLKSLHESLRER"
     assembly_gap    5917..5927
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5928..7957)
                     /locus_tag="PK614_02375"
     CDS             complement(<5928..7957)
                     /locus_tag="PK614_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRB81087.1"
                     /translation="MLCLLMVARFHDLPVDGAQIQHQFGQSDEALTTRELLRAAKHVG
                     LKAGLVTASWQELPGTPLPAIVQRIDGRYVVLAKVQDDKVLIQDPLESRPLIQSKDDF
                     SKAWSGGLLLCTKRAGLGSQDLVFDLTWFIPAVLKYRRLLGEVLLASFFLQLFALLTP
                     LFTQVVIDKVLVHKGFTTLHVMAVGMLALTLFDALLGGLRTYLFAHTTNRIDVGLGAQ
                     LFRHLLALPLAYFEARRVGDTVARVRELEHIRQFLTSNSVTVVLDVLFILVFLAVMWI
                     YSPVLTLVVLASLPLYAVLSVAITPTIRARLNEKFNRGAENQSFLVEAVSGIQTVKAL
                     AVEPPLQRRWDEQLAGYVQASFRATSLITMAGQAGTFIQKSTTIAVMWVGAYQVIDGA
                     LSIGELIAFNMLSGQVTGPLLRMVNLWQEFQQVGISIQRLGDVLNTRPEPSYNPNRTT
                     LPQIAGQITFDDVTFRYRPDGPPVLQRVSFTLQPGQIIGMVGRSGSGKSTIAKLVQRL
                     YVPERGRVLVDGVDLAQVDPAWLRRQVGVVLQENFLFNRSVRDNIALTDPGSSMDRVM
                     HAAKLAGAHEFILELPEGYDTIVGEHGCAISGGQRQRIAIARALVANPRILIFDEATS
                     ALDYESEAIIQHNMAQICKGRTVVIIAHRLSTVRPAHRIYVIDRGQLVEQGT"
     gene            complement(8044..8379)
                     /locus_tag="PK614_02380"
     CDS             complement(8044..8379)
                     /locus_tag="PK614_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81088.1"
                     /translation="MAESHHPMQRGDKSVAVNVRLKPVSPSDQPILVNYTHVGLSDGL
                     AYVDFGFVEPALLSAVATRAKQGEVTPKQVEGISAARVALPLDSVVRLYQQLQRVLVS
                     LQPPKTKQS"
     gene            9009..9941
                     /locus_tag="PK614_02385"
     CDS             9009..9941
                     /locus_tag="PK614_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRB81089.1"
                     /translation="MIWKSTPELTHGRIGFSLTYIQKLERQDGDKWVAVPLTPMVHHE
                     PNKCQEPTGQARRRFFTRRSLLHPALPPPHSLRSGIGACYDKTTAVMTRADDLKPDPY
                     NTPQVHTMYTPINKLLMQGVVDSWIRLSASLAVWAFCLTVTACAGVEHKDTLPTVASV
                     DLARYAGTWYEIARLPMWFQRHCVDSKAMYTSRPDGSIGVHNECVTDSGGVDQAEGVA
                     TVIDPKTNARLTVVFDNFFARLFGSSRDGNYWILALDPEYRTALVGTPDRRFLWILSR
                     APQLEEVTYQRLVEQAKRLGFPTADLIRTRRPAS"
     gene            complement(10016..10777)
                     /locus_tag="PK614_02390"
     CDS             complement(10016..10777)
                     /locus_tag="PK614_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB81090.1"
                     /translation="MTIMPQRILIADDEETFRESTAEILAEAGYACSVARDSAEAESL
                     LENGYDLLLADVRMPGNAHLEFLENVSRRHPELPVVVITGYPTVGTAVESFRLSIVDY
                     LIKPVGVDDLKRAVERGLRRRTLARAVKDAMTETAKVAGIFDQLGQSMREFGRGEPQV
                     EWSAHEYMAQAMGHIAQLSALVAQTLEASRGSRPQAPTDVCALMQCPRLDRYEAAIQD
                     AVEVMERTRSSFKSKEIGALRQRLETLLREGRRQS"
     gene            complement(10743..13517)
                     /locus_tag="PK614_02395"
     CDS             complement(10743..13517)
                     /locus_tag="PK614_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB81091.1"
                     /translation="MSVAVRWFHAVRSPLYSGMTERRWYALCAVILVVTAAIDAYTPL
                     GIAAGVGYLFAVVVAGMVYEVRFPWRVAAVSIGLTVLGFFCSPAGGVVWIGAMNRVGL
                     AMAILLTAWIVGQWSMATRSRSHDLSVRLQSILTHSQTSIFLKDLQGRYLEVSEQFLS
                     LTGFSAEMVIGKTDRDIFPPELVAIYREHDAQVIKAGAAMNFEETVPLSDGLHLNIVC
                     RFPLRDGQGSIVAIGGIATDITARLQAEAARSEAQERFDLVVTATQTGIWDWDLHTNH
                     MYYCPLWKASLGYQEHEISNSPTEWESRLHPDDKARAFALVDDYLFGRIGAYELEHRL
                     RHKDGSYRWIQTFALLQRDADGRPRRMTGSHVDITERKRAEDALTQSESTLKSFFNSG
                     AMMMGIVELLDGDILHVSDNRCVASFFGTIPELMQGRLASELGAPPDILALWHRSYEE
                     SERTKQPVRFEYVHPQKGSGVELRLSATVCWIGIGLSGRSRYSYVVDDVTEARCLAEA
                     LGQTAERYHGVVTALAEGVVLHDEQGRIIACNPSAERILGLTADQLMGRTPVDPGWQA
                     IHEDGSHFAPDQRPPMVTLRTGRPCSNVIMGLSRPTGEQRWISINTQAILRAGEERPY
                     AVVSSFHDITERRRAEQALLEVQSQLEQRVRERTARIQQLESQRSQAEKLAALGHLAA
                     DIAHEVNNPLAGITNAFHLVKQGIGSEHRHYRFVDLIDREIQRLTAIVKKMYTLYQGL
                     PATERQVTDIAELLQDVATLLEHKMAAKRLRLRTEVDTPLQVVDVSRSDLFQVLLNLV
                     QNAIDASPPASEVVLNVSEREGLLRWSVTDCGTGIAPDILPRIFEPFFTTKPDAGWQG
                     LGLGLSVSRGLVQAMGGRIEVQTELQRGSTFTVVYPLEPPDMSGERAAGVNADKEQKD
                     DDYATTHSHC"
     gene            complement(13762..13839)
                     /locus_tag="PK614_02400"
     tRNA            complement(13762..13839)
                     /locus_tag="PK614_02400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13801..13803),aa:Glu,seq:ttc)
     gene            14002..15972
                     /gene="glgB"
                     /locus_tag="PK614_02405"
     CDS             14002..15972
                     /gene="glgB"
                     /locus_tag="PK614_02405"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRB81092.1"
                     /translation="MDPLVNHRAGEHLDPATPATRLTDERRSLLTANDLYLFNEGSHF
                     HLYDKLGAHPATLQGVEGTYFAVWAPEAEQVSVFGTFNHWDPTRHLLHPCQFSGIWEG
                     FVAGVGVGALYKFHIRSRHHGAELIKTDPFARLNEIPPKSASVVWNLDYTWQDSNWMH
                     TRARHNALDAPISIYEVHLGSWMRVPGEGNRSLSYREAAPKLIEYVQRLGFTHVEFLP
                     LMDHPFFGSWGYQTTGYFAPSGNYGTPQDLMYLIDQLHQHDIGVILDWVPSHFPTDEH
                     GLSRFDGSHLFEHADPRQGLHPDWGTAVFNYSRNEVRSFLISSALFWLEQYHADGLRV
                     DAVASMLYLDYSRKEGEWIPNRHGGRENLEAITFLRQLNEEIYRRHPDVQTFAEESTS
                     WPSVSRPTYAGGLGFGMKWDMGWMHDTLEYMALDPVYRKHHHRNLTFRMLYAFQENFL
                     LPLSHDEVVHGKGSLLGKMPGDDWQKFANLRALFGYMYAQAAKKLIFMGGEIGQWREW
                     AHDDSIDWNLLQYPPHQGLQRWVADLNHLYRAEPALHEFDFDPRGFEWIDCQDVDAGV
                     ISLLRHGRTPQQHIAVVCNFTPVPRLQYRVGVPQGGYWKELLNSDASMYGGTGLGNLG
                     GLSAEPIPAHDRTHSLAMTLPPLSVLFFKAPS"
     gene            16075..16560
                     /locus_tag="PK614_02410"
     CDS             16075..16560
                     /locus_tag="PK614_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81093.1"
                     /translation="MAIRSRTASMWHGLVLLTACLTLANCSHDIHEKRADTVKDHVEA
                     FYDHLTHDRVAAAVRENEAIEHLSSQLGDIISRRVNRPGTNQVDREWTDLRTANETAA
                     QNWLALGQYLSIKKQYAQSRATYQRVIDTYTGTTERTYREQAARAIRDLDILTPPPSS
                     H"
     gene            16575..17138
                     /locus_tag="PK614_02415"
     CDS             16575..17138
                     /locus_tag="PK614_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81094.1"
                     /translation="MRRPTRGLKYRLTIMLLGTYLVGAGCVHHIHVTSPSSDKAAAPI
                     PATVQLEVPFLAIEGADHMPGIPLLEWPSKDLQQTIVEYFTQRQTFTSIGTEAGDMRL
                     VVKAWLMLRAPDRYLYRVHLESDLAFSGQAPLKTYAAEGEALGSSVRWVTASDQEPIA
                     EATAQALHQLATQIEQDREFVIKATHR"
     gene            17530..18726
                     /locus_tag="PK614_02420"
     CDS             17530..18726
                     /locus_tag="PK614_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HRB81095.1"
                     /translation="MSKRLILCFDGMWDLPPDPGRQLPSSSTPNTQTLHNSHSHLSPA
                     TNVLRLYHSILPRTEDGRAQQKWYDSGADIPWFYRFRPGSFGYGVDQTILHAYAYLAA
                     TYEPGDELFVYGFSRGAYTARSLVGLLTTAGLIPAGLLHTDLVERVREVVSHPSPSTT
                     TSTALRQCLHEMILDPSNQALDDAYRLYRKGHGDILAGIPTSARRRGTLEVPITLLGL
                     WETVGPLGIPTNALKWLNDHRYNFHDTELSSIVKQAYHALAIDEHRADHNATLWTSPA
                     CPGQSIEQRWFAGAHGDLGGAYPERDLADVALAWLQRHSMEKGLAIETRLVPLQPNAL
                     SPIHDSFSECFGRIRKWFHSRFYRPVMQTGTNTEVLDSSVHTRLIHTPHYRPKNEGLK
                     SVLHFE"
     gene            19065..19427
                     /locus_tag="PK614_02425"
     CDS             19065..19427
                     /locus_tag="PK614_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81096.1"
                     /translation="MTRSSHRRFVVHCILSAAAMGCAEKGRIIESGLVGAPHPTSHSL
                     IDGDYLALKLQVPEDRRPVSVGLARVTWAQGNRFGVELLMMDADERARLNGFLEEHLP
                     LELEFQDSHQELTITAAE"
     gene            19663..19908
                     /locus_tag="PK614_02430"
     CDS             19663..19908
                     /locus_tag="PK614_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81097.1"
                     /translation="MTCSRCNGCMVEDFLLDMEDSSGPMWLQAKRCMNCGNVAEKVLE
                     HNRQTQGAALHSAKSAPTIPNSEHAHFGSGGMAQLAA"
     gene            20499..20870
                     /locus_tag="PK614_02435"
     CDS             20499..20870
                     /locus_tag="PK614_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81098.1"
                     /translation="MTSTAKTVVRDAAIVYGLTFAAGLCMAAAGITLESNSSTAYLCN
                     LLSGVLGFTLVGTRLSANRAEHLAWVAATLWTFNLTNIVLGLQTSSAWIHSGLTILLM
                     ASLGGSLAMILTLTSAADRRT"
BASE COUNT         4464 a         6450 c         5595 g         4406 t
ORIGIN      
        1 taacaggacg tgccggttcc tcatgccacg ctcctttcga atgaatttct gtagctcgca
       61 tcgttcgcta catcacctcc ctggacatgc cagtcacgga gggtggggag cagcgattga
      121 atcacttggc ggtcgccggc agaaaaggcg ttcaccaggt aatactgaaa atcggtgcag
      181 cccttggcct gctcgagcga agccgccaca tccgcccgat cgaattggag gccgcccgtc
      241 accggagtaa agagttcctt gttggcattc gtggcattga ctgtgtcttc gtagtacatc
      301 tgcatggcga acgcgatcag cgccttgctg accaaattcg tggcaggggt gggaccgtcc
      361 gccatcgtca acccaccgtc ctgcgccagt ttccacagat cctcggtgaa ccgggtcacc
      421 atggcgttgc cctgttcatt ctgcacaagc cgttcgagaa agttgcggtt atcagcgtca
      481 gtggtcggat gcgcgtacaa gttgtcgtcg aagatcatct ccaccagatt cgtgagcttg
      541 aacgtcacat cgttcaacgc ctgtccagac tgcgcagact gcatactctg gagaaaggcc
      601 gtcagcaagg ctaccgaatg aagatcatcc cccgacacac taggagcatg gatggggatg
      661 tgggaggatt ggccgatacg atcttggatg ttccagggca ccccggacag aaactcgccg
      721 tccacattga tgttcttcac caaactggtg ttggggatct cgcccatggg cagggccgcc
      781 cgtacattga gaaagtccgt cagcccttgc aatgcatcgt cgctgtactt gcgacccccg
      841 ctcagatcga gttggttaac taagtagctt cgaagattcg cagccacgtc tggagcgatc
      901 aggctcagtt cagcggagtt ggcaaacggg gcttggtcaa acgtgactgt ttgcttcccg
      961 aagaacacgc ccatcaaggc tgctaagcct ccgcccaaac tgtggccagt aaacgtaata
     1021 gtagcgttgg gattggccgc cttcacttgc aggtaatatt cggcggcctg ctcgagctga
     1081 acagatccaa tacccgtggc caatccgata tcagccgcga tgtcgcctgt aaagtcgctg
     1141 ggattagtgc ctgcatagga gatgacgatt tcgttgccac gctgacacgc gaccgcttca
     1201 aaaccggaac ttggatcgag acgatagccg tttggacttg gcagtgtcgc ccaacccatt
     1261 gggattggaa atttattact gtctgggcgc gtagatatgt aggaagcccc tgccatcatt
     1321 gcataatcga taagttgcac cgtcatgatc acctctatat ttgagtggcc aacgaatgtt
     1381 tagttgaggt ctcgctccct catttactta ccgatggagc cgccagagcc accaacccat
     1441 actcctttgt ggaaaaccat ctctagacaa ttcaccgcag atgcctcaga tcctggcttc
     1501 accggctccc gtaagatggc cttatattct ggctgtatga gctggctatt ctgtttctga
     1561 atatgctcca cagagaccac tccttgcttg accatggcgt ccacaaactg ccgactcaga
     1621 ccgagcacta cattcatggt ggtgaactca gtgggtaagt cttcgagtgg aatgcgctgc
     1681 caagtagtgc catcgtattt gaagaacacg tagggcggat tgggccggcc ccacttgttg
     1741 tacgacagac acaagttcgg ctccgcaaca atgtagggcg tgccgttcag cacatgcaca
     1801 gccagtaggt gaaagttagt gcgcccaagt tcctttccat attcactcgt ccaggtcatg
     1861 gtcttgttgg tatgaggcag cgtgaacgtc accgtgtgtt ctttaatcgg cgatggttgt
     1921 ccgatctcat gccgcccccc ataagtctgt gaccgcgtga tcaccagctt gctcccgtca
     1981 tgcagcagca cttcttcttt ccagctatcc ccaccgaacg gaagagcgca ccatcgcgca
     2041 cgcactcatc tcaatagcaa cgatcagagc gctaaccagg aagagccatc gcaacctctg
     2101 tggcttcgtc atgccgccat cctttctctc gagttctgct cactaaaatg cgtgctcaca
     2161 caattctcat tgccccggcg gatgttgcct tcgcgaaatt cgcctccgca attccactac
     2221 cgaccagccc aactcgctgc tgactcctcg cctgctcccg gagtgatgta cgcctctgcc
     2281 taaacgcggt tcaacgagaa aaaggtaccg gtaccttttt gttgttctcg gcaataagtc
     2341 cgcctcaaag ggacttgcct caccgctgtc cgccccgcac gcgatgcagt ccgatcttga
     2401 ttgagagatt acggacgtga ttcttgacgg tctgggttcc aatgcgcaac gcaactgcta
     2461 tgtctttatt attcagaccg ttccggcgca atcggaggat ctccacttca cgcggggtca
     2521 gcccaaatcg ccgctggtcc aacccatcgt cccaggacgc cgccgacttc acaaggcttg
     2581 ggtcagcagt ctgaggatct gaagatcgac tcaagtgaag tcggcttagg cgatgcacca
     2641 cctgcagcag accctgaaag gaactgtcgc ctaggaccat agggatcgag gccgaccaac
     2701 cctgatcctg cgtacgccga gacctcatgg tcacaagcgg tctgccgatc aggagaatcc
     2761 gagccgccgg aaacctggtg tgtacccgca acgcctggcc ataggtttcc cgagatccgg
     2821 cttgccctcc caggagaacc acgtcggggt cgactgtttc ctgctgaacc aattcctcgg
     2881 catcggccac gcaggccatc acaatcacca cgtacggcga taccgccaac gccatggtca
     2941 gtacgtcaca gaatagtcgg tctggatgca cgatcagaat cttgacctcg tgctgacccg
     3001 agacggacgc tttctgttct tcctggacga tgtctccgtt cacccccagc cggctcagtg
     3061 ctgccccatt cattgcatca acgtccttct ctcctgacct taactcaacg actccagcgt
     3121 gccggtcgcc cgtgcgtagt tcaccctggc ggcgttcaat tcgaacaacg ccccgacaag
     3181 gttctctctc gcccgcgcca tcgacgtgat ggcggtgatg acatcaaatt ggctggcggc
     3241 ggtcagaacg gcgtaacgtt ctcttgccag ggccgtttct ttcgtcgccg cctgcaagcc
     3301 ggcctgggcc agcgcggcct tgtctctggc cgccgccaat gcgatgcggg cttcattgac
     3361 ctcggcgcgc acttgattca gcaccacctg cattcgaaag gcctcctgcc gcacctgact
     3421 ccgggccgtg ctgatccgtc cctctcgctg gccaccgtcg aacaagggaa tctggagtaa
     3481 gagtgccata ttgtacgtat ccaggctatt gttccaccga ttgccgatca ggccggtatc
     3541 cccctgcgcg accaaggacg gcaggcgctc cccggtcacg gaggaatagc tgagttccgc
     3601 cgaccgaatc cgcttggctt gcgcctgcac ttcggcccga ttggtgacgg cctgctccca
     3661 ggccgcctgt gccgctgccg cttccggcac ctggggttgt aagggatccg tcagcgtcat
     3721 tctgacctcg aaggtaagcc ccaagagatt cccgagggag ttcttagccc gctccaggtc
     3781 ggcctgcgcc gcgacgacct gctgctgttc gttggccagt tgcccttcca accgtgcgac
     3841 ctccaatccg gtcgccgccc cttcgtgctg ccggaccttc gcggtcgcca acaattcgcg
     3901 gatcaattgt tgattggcct gccgcatggc caacgccgtg gccgctttca ccccttcgag
     3961 ataggccagc ccggtactgg ccatcgtatc gaaccggctg ctctgggcat cgaactcggc
     4021 gacctgaagc gcttcccgcg aagcccgcca gcgctgaatg agactgaggc taaacaggtt
     4081 ctgcgacgca ctcacgcgcg catcaaaaat gctgaagggc tcggtccgga ccggcgcgag
     4141 accgaaggta ccgaggaact gtgtctgttg gctttgccgg acattggcag agagattcgg
     4201 cagcatagcg cccaattgag tggtgacctc acccttggcc gcctcgatgc gttctttgga
     4261 cagtagcacc gaaggattct gctgagctgc tgcggccatc gccgcacgca gactcagatg
     4321 gatctcctgc acagggagcg tcgtcatcgg ttctcggtca gtggctcttg cctgctgaac
     4381 agtgctcaag gacaccgtca gactcacgac gaggagaact cgccctacta tgaatccaac
     4441 gcgtggcatc cgccttaccg ctccctcagg ctctcgtgca acgatttgag tacgggactc
     4501 agcaggtact cgatgacgcg ccgtcggccg gtcttgatct cgacggtcac ggccatgccg
     4561 ggagacaaat gcaccaattt gccttccact ggaatcgtcc cacgcgccac actcacgcgt
     4621 gtggcaaaga ccaagccgtc tgccggcttg tctttgttga ccggcacagc gtcatctgag
     4681 accgtaagca cggtgccggg gatggtgccg tagagggtga acggaaaggt ctcgactttg
     4741 atctcggccg actggccttc tttgacgaag ccgacatcct tattctccaa ctgcgcttcg
     4801 acttcgacgg gatggtcttg cggcactacg atgagcagcg gttgcgccgg cgtcacgacg
     4861 cccccgaccg tatgcaccgc gagctgctgc accacgccat cgatcggact cacgagccgc
     4921 tggagttccg ccttctgccc agctttcgtc acttcctgtg agagggaagc ggctttggtt
     4981 tcaagcgccg acagttcggc ctgtttcgtc tgctggaatt ctgagaccag ggcccgtcga
     5041 tttttttcgg cttccgccaa tgccgcctgg tcttgccgga gcttgtgctg ctgtccggcc
     5101 aattcctgca acttgtcgat acgctgccct tctgcttgaa ggtaatcgag tttcgtgacg
     5161 gcatcccgct ccattaaccg tttgaacgcg ccagcccgct ccgtttccat gggcactgtc
     5221 gcttccagtc gcaggatatt ttccctggtg gcgtcaatgg ctgccctgcg ctgcgtgatg
     5281 atatgttggg cggcgtccac tcgcgcctga tactcggcca actgatcatg aaggagctgt
     5341 tgctggagcc ggacatatcc gggatcgctc ttcagtggga ccacaagcgt gggcacgcct
     5401 gcgatgaggg cctggaggcg cgcggcctcg accttggccg cgcgatactc attgaaggcg
     5461 cgatcacggt ccgcttgatt ttgggtcgga tcgagttcaa tcaggacatc cccttgcttc
     5521 acgacttgac cgtcctgcac ccggatcgcc gtaatgacac cactttcata gggctgaatg
     5581 attttcgaat agccgttggg gataatcttc ccctgcgccg tcgccacgat atcgatcctc
     5641 ccgaccgagg cccagaccac ggagaccgtg aagagcgcca tgatcgtcca gagcatcgcc
     5701 cggccgatcg gggatggtgg cgcgtcttga atctccagtg ccgccggaag gaactcgacc
     5761 gatctcctcg aaggggtggc cgcaaccggc tgtcgtctct ccgcctccca ggccgcggac
     5821 cagacctgcc accatcgcat catgctcgtc cgcttcatgc agccttcccg tcctgatggg
     5881 cgtgcagtcg gtgatacatg ccttgacgtt tcagcannnn nnnnnnntgc gtaccctgct
     5941 ccaccagttg gccccgatcg atgacataga tccgatgcgc gggccgcacc gtgctcaaac
     6001 ggtgagcaat gatgacgacc gtgcgcccct tacagatctg tgccatgttg tgctgaatga
     6061 tcgcttcgga ttcgtagtcc aaggcgctgg tcgcttcgtc aaaaatcaaa atacgcggat
     6121 tggcaacgag ggcacgagca atggcaatac gctgacgctg cccaccggag atggcgcaac
     6181 catgctcccc tacaatcgtg tcatacccct ccggaagctc caggatgaac tcatgggcac
     6241 ccgcaagttt ggccgcatgc ataacacggt ccatcgagct ccccggatcg gtcaatgcga
     6301 tgttgtctcg gacggatcga ttgaatagaa agttttcttg cagcaccacg ccgacttgtc
     6361 gacgcaacca ggccggatcg acctgtgcaa gatccacccc atcgaccagc acccggcccc
     6421 gttccggcac gtagagccgc tgcaccagct ttgcaatggt gcttttgccc gacccggagc
     6481 gtcccaccat cccaataatc tgacccggct gaagtgtgaa tgagacccgc tggagaaccg
     6541 gcggtccatc tggacgataa cgaaatgtga cgtcgtcgaa cgtaatctgc ccggcgatct
     6601 gcggcaaggt tgtgcggttc gggttgtatg agggctcggg tctggtgttc agcacgtcgc
     6661 ccaagcgttg aattgaaatg ccgacctgct gaaattcctg ccagagattc accatgcgca
     6721 acaaaggtcc ggtcacctgc ccggagagca tgttgaaggc gatcagctcg ccgatactca
     6781 gcgccccatc gataacctgg tacgcgccga cccacatgac ggcaatggtc gtggattttt
     6841 gaatgaaggt ccccgcctgg ccggccatgg tgatgagact ggtggcgcgg aaactggcct
     6901 ggacgtaccc ggccagttgt tcgtcccatc ggcgctggag gggaggctcg acggccagag
     6961 ccttcacggt ttgaatgccg ctcacggcct cgaccagaaa cgactggttc tccgccccgc
     7021 gattgaattt ctcattgagc cgtgcgcgaa tcgttggcgt aatggcaacc gacagaacgg
     7081 catagagcgg cagggacgcc agcaccacca acgtcagcac agggctgtag atccacatca
     7141 cagcgagaaa cacgaggatg aacagaacgt ccagcacgac tgtcaccgaa ttactggtga
     7201 gaaattggcg gatatgttca agttcgcgca cacgtgcgac cgtatcgccg actcggcggg
     7261 cttcaaaata ggcgagcggg agggctaaga gatgccggaa cagttgcgca cccaaaccca
     7321 catcgatccg attggtggtg tgggcaaaca ggtacgtacg caacccaccg agtagcgcgt
     7381 cgaatagggt cagcgccagc atgccaacag ccatcacgtg cagcgtcgtg aagcccttgt
     7441 gtaccaagac cttatcgatg acgacctggg taaagagcgg ggtgagcagc gcgaagagct
     7501 ggaggaaaaa cgaggcgagc aacacctcgc ccagcagccg tcggtacttg agtacggcgg
     7561 gaatgaacca ggtgagatcg aagacaaggt cctgcgaccc gagacccgcg cgtttcgtac
     7621 agagcagcag cccaccggac caggctttgc tgaagtcatc cttggattgg atcagcggtc
     7681 gcgactccaa cggatcctgg atgagcacct tgtcgtcctg caccttcgcc agcacgacgt
     7741 accgtccgtc gattcgttgg acgatggccg gcaacggtgt ccctggaagc tcttgccaag
     7801 atgctgtaac cagaccggct tttagtccga cgtgtttggc cgcacgcagc aactcccgcg
     7861 tcgtcagtgc ctcgtcggac tgcccgaatt gatgctgaat ttgtgcaccg tcaacaggca
     7921 ggtcgtgaaa ccgggcgacc atcagtaagc ataacaagcc agtgtcccga aggggttgcg
     7981 gcgcagggca agatactgga ttgggtgtca acgtatccat gaaaagaata tcgggtagac
     8041 ggattaggat tgtttcgtct tgggaggttg gagactgacc agcacccgct gaagttgctg
     8101 gtagagacgc accacggaat cgagaggtaa ggcaacacgt gccgcgctga ttccctcgac
     8161 ctgtttcggt gtgacttcgc cttgcttcgc acgcgtggcg acggcgctca acaaagcagg
     8221 ctctacaaac ccaaaatcga catatgccag tccgtccgag agaccaacat gagtgtagtt
     8281 cacaagaatc ggctgatccg atggggaaac gggtttaaga cgaacattca cggccaccga
     8341 tttatcccca cgctgcatag gatgatgact ttctgccatg ctgctcctcc tttatttcac
     8401 taagcgtgat gcaatcctcg caagtcgccc ggactctcgc aggacagagg cgatgttatg
     8461 gcgagactgc ccggcgcact attccccgga ggttgggcag aggcaaaaac tttggtaggc
     8521 caatacattt ccctaggtat attgaggtta ccccacgcag tgaaggttag aatgcagggc
     8581 tgaacaatcc gaaacgtgta ggacaaatgc ggactcgcca ggagtgacac tgcgccaatt
     8641 ctccgattca gaacgtgtca ctatcattcg ctgggttgga gaagatgcag acatgcatat
     8701 gccaccatgt cgcagattat ggggcttggc accacatgga tatcgcgatt ctcccctaca
     8761 atcagtatca gcggcggacc ggctcttgtc acagccaggg cgttccatct accgctcgta
     8821 gactcaccgg gaacgcactt gcgtcctcag acaacgggct tatcctaccg ccccgttcgc
     8881 ggacttcctc cagtggtttc atcagttccc tcctcaaacc cgaggggatt ttggttcgct
     8941 ttgaatcggc ggagctcctg ccaggctggc atgtgggaac tgtggggctg acgaaagacg
     9001 gatgtgtgat gatctggaag tcgacgcccg agcttaccca tggacgaatc gggtttagcc
     9061 tgacctacat tcagaagctt gagcgtcaag acggagacaa gtgggttgcg gttccgctca
     9121 caccgatggt gcaccatgaa ccgaacaagt gccaagagcc tacggggcaa gcacgacgtc
     9181 ggttcttcac tcggcgcagc ctcttgcacc cggcgttgcc tcccccccat tcgctacgca
     9241 gcggcattgg tgcttgttat gataaaacca cggctgtgat gacccgtgct gacgatctga
     9301 agcccgatcc ttacaacaca ccccaagtgc acactatgta cacaccgatt aataaattgc
     9361 tgatgcaagg cgtggtagac agctggatcc gattgagcgc gtcgttggcg gtttgggcat
     9421 tctgccttac cgtcacagcc tgtgcgggcg tggagcacaa ggacacattg ccgacagtgg
     9481 cttcggtaga tctcgcccgc tacgcgggaa cgtggtacga gatcgcccgg ctaccgatgt
     9541 ggtttcagcg gcactgcgtt gactcgaaag ccatgtatac cagccgtccg gacggcagca
     9601 tcggcgttca caatgaatgt gttaccgaca gtggcggagt tgatcaggcg gagggtgtgg
     9661 cgacggtgat cgaccctaaa accaatgctc ggctcaccgt ggtcttcgat aacttcttcg
     9721 cgcggctctt cggttcatca cgggatggga actattggat tcttgcgctt gatccggaat
     9781 accgaaccgc gttggtcggc acacctgatc gtcggtttct ctggattctc tcgcgagcac
     9841 ctcagctgga agaggttacc tatcagcgcc tcgtcgagca ggccaagcgc ctcggctttc
     9901 caaccgcaga tctcattcgc actcgccgac cggcatcatg atcgcctaag cttgccggcc
     9961 gccctgagga ccacggctgc aacgggaagg ctcgcttcgc acttgccaca cagagtcagc
    10021 tctgcctgcg tccctcgcgt aggagggttt ccagccgctg ccgcaacgcg ccgatctcct
    10081 tcgacttgaa cgagctgcgc gtacgctcca tgacctcgac ggcgtcctga atggccgcct
    10141 cataccgatc caacctgggg cactgcatca atgcacagac gtccgtggga gcttgaggcc
    10201 tgctcccacg cgaagcttcc aacgtttgcg cgacgagtgc cgacaactgg gcgatgtgtc
    10261 ccatggcctg ggccatatac tcatgggcgc tccattccac ctgtggttct ccccgcccga
    10321 attctctcat cgactggccc agttgatcga aaattcccgc caccttggcc gtttcggtca
    10381 tggcatcctt taccgcacgc gcaagggtcc ttcgccgaag cccgcgctcc accgcccgct
    10441 tcagatcgtc cacgccgacc ggtttgatca ggtagtccac aatcgagagg cgaaacgact
    10501 cgacggcagt cccgaccgtc ggataccccg taatcaccac cacgggaagc tcgggatgtc
    10561 gcctggaaac gttttcaaga aactcaaggt gagcattccc cggcatccgg acatcggcca
    10621 gcagcaagtc gtagccgttt tccagcagac tctcggcctc agccgaatct ctggcgacac
    10681 tacacgcgta tcctgcctca gcgagaattt ccgccgtaga ttcccgaaac gtctcttcgt
    10741 catcagcaat gagaatgcgt tgtggcataa tcgtcatctt tctgctcctt gtctgcattc
    10801 actcccgccg ctcgctcccc cgacatatcc ggcggttcca agggatacac caccgtgaac
    10861 gtcgacccac gttgtaattc agtctggact tcgatccgcc ctcccatggc ttgcaccagc
    10921 ccacgcgaca cagataaccc gagtccgagc ccctgccaac cggcatcggg cttcgtcgta
    10981 aaaaatggct cgaaaatacg gggcaatatg tccggagcta tccccgtccc gcaatcagtc
    11041 acgctccatc gcagcagccc ctcgcgctcg gacacgttca acactacctc actagcgggg
    11101 ggagaggcat cgatggcgtt ctgcacgaga ttgagcaaca cctgaaagag atcggaacgc
    11161 gacacatcga ccacctgaag gggcgtatcc acctctgttc gtaaccggag tcgtttcgcg
    11221 gccatcttat gctccagcaa tgtggccacg tcctggagca attcggctat gtcggtgacc
    11281 tgtcgctcgg tcgccgggag gccctgatac agggtgtaca tctttttcac gatcgccgtg
    11341 agacgttgaa tttcacgatc gatgagatcc acgaatcgat aatgtcgatg ctcgcttccg
    11401 attccctgct tcaccaggtg aaacgcattg gtaatccctg ccaggggatt attgacttca
    11461 tgggcgatat cagccgcgag gtggcccagc gccgcgagtt tttctgcctg agaccgctgg
    11521 gattcaagtt gctgaatcct cgccgttcgc tcacgcacgc gttgctccag ctgactctgc
    11581 acctccagca aggcctgctc cgcccgacgg cgctcggtaa tgtcatgaaa cgagctgacg
    11641 acggcgtaag gtcgctcttc accggctctc aggattgcct gcgtattgat tgagatccat
    11701 cgctgttccc cggtcggtcg agagagaccc ataatgacat tcgagcacgg gcgaccggtc
    11761 cgaagcgtga ccatcggggg gcgttgatca ggagcaaagt ggctcccatc ttcatgaatc
    11821 gcctgccatc ccggatcgac cggcgtccgc cccatcagct gatcggccgt gagcccaaga
    11881 atccgctccg cgctcggatt acaggcgata atcctccctt gctcatcatg caggacgaca
    11941 ccttctgcca gcgccgtcac cacaccgtga taccgctcgg ccgtctggcc cagcgcttct
    12001 gccaagcatc gtgcttccgt gacatcgtcc accacgtagg aataacgcga ccgcccgctt
    12061 agtcctattc caatccagca gacggtggcg gaaagacgca actcgacccc gctgcctttt
    12121 tgcgggtgga catattcgaa tcgcaccggt tgcttcgtcc gttcgctctc ttcgtacgat
    12181 cggtgccaaa gagcgaggat gtccggtggg gcgccaagct ctgacgcgag tcgcccctgc
    12241 atcaattccg ggattgtgcc gaagaagctc gccacacagc ggttgtctga gacatgcagg
    12301 atatcgccgt cgagcagctc cacaatcccc atcatcatcg cgccgctatt gaagaaactc
    12361 ttcaacgtgg actcactctg ggtcaacgcg tcttcggctc gtttccgttc ggtaatatcc
    12421 acgtgcgacc cagtcatccg gcggggtcgt ccgtcggcat ctcgctgcaa cagggcaaag
    12481 gtctgaatcc agcggtagct gccgtctttg tgacgcaggc ggtgctccag ctcgtatgcg
    12541 ccgatgcgcc cgaataaata gtcgtccaca agcgcaaatg cacgagcttt gtcatcggga
    12601 tgtaatcggg actcccactc cgtcggtgag ttggaaatct cgtgctcctg atatcccaga
    12661 ctcgccttcc acagcgggca gtaatacatg tggttggtgt gcaggtccca gtcccagatt
    12721 ccagtctgtg tcgcggtgac aacgagatcg aaacgttcct gagcctcgct tcgagcggcc
    12781 tctgcctgca atcgggcggt gatatccgtg gcaatcccgc cgatggccac aatcgatccc
    12841 tgcccgtcac gcaacggaaa cctgcagacg atatttaagt gcaggccatc gctcagcggc
    12901 acggtctctt cgaaattcat ggccgcaccg gccttgatga cctgggcatc atgttcccga
    12961 tagatggcga ccaattcagg ggggaaaatg tcccgatcag ttttcccgat caccatctcg
    13021 gccgagaagc cggtcagaga cagaaactgt tcgctcacct ccaggtatcg cccctgcaag
    13081 tctttgagga agatacttgt ctggctatgt gtgaggatgg actgcaaccg aacagagaga
    13141 tcgtgggagc gactgcgggt tgccatgctc cattgtccga cgatccaggc cgtcaacagg
    13201 atggccatgg cgaggccaac gcgattcatc gctccgatcc atacaacccc acccgctgga
    13261 gaacaaaaga atcccagcac ggtgagtccg atcgagacag ccgccacgcg ccaaggaaat
    13321 cgtacctcat acaccatccc ggccacgacc acggcgaaca gataccccac cccggccgca
    13381 atccccaatg gcgtgtacgc atcaatagcc gccgtgacca cgaggatgac ggcacagagg
    13441 gcataccatc gcctttcggt catgcctgaa tacagcggtg accggacggc gtgaaaccag
    13501 cgaacggcta cactcatcac agaacgactc cgtacgtcac tcaatcgtaa cgacgccgga
    13561 tcatgaggcg ggtaacggtg cagtttcgtg gtcgagatcc cggcggcagc gacgatccta
    13621 cctgccggcc ctacagcgat cctctcccac gacaagaacc atcctccatg aagccgcaag
    13681 aagctttata tgtgagatgg ctgtactgtc tggaaagaat gttcccgaca aggttgagcc
    13741 aacgaataac cgggagaagt gtggtgtccc caacgggatt tgaacccgtg ttactgcctt
    13801 gaaagggcaa tgtcctaggc caggctagac gatggggacg aagagaattc cgtatgagcg
    13861 ggttgattca taccatactt gatcgagagg tgtcactagt ctggagcaag atcccgaaga
    13921 gtcccacgat cggaaccatt gccggccgac cgagccgccg gccgattgac gatctgccct
    13981 caaccacagt actctgcacc tgtggacccc cttgtcaatc atcgcgccgg cgaacatctc
    14041 gatccggcta cgccggcaac gagactcacg gacgagcgcc ggagtctgct cacggcgaac
    14101 gacctctacc tctttaacga aggttctcat ttccacctgt acgataaact cggcgcgcat
    14161 cccgcgacct tgcaaggtgt cgagggcacg tactttgcgg tgtgggcgcc ggaagccgag
    14221 caggtctcgg tcttcgggac gttcaaccat tgggacccca ctcgccacct actccacccc
    14281 tgtcaatttt caggcatttg ggaaggtttc gtcgccggtg tgggagtcgg cgcgctgtac
    14341 aaatttcaca tccggtcacg gcaccatggc gcggaactca tcaaaacaga ccccttcgca
    14401 cgactgaacg aaattccgcc gaaatccgca tccgtggtct ggaacctcga ttacacctgg
    14461 caggacagca actggatgca cactcgcgca cggcacaatg cgctggatgc tccgatcagc
    14521 atttacgagg tccatctcgg ctcatggatg cgggttcccg gcgaaggcaa ccgctccctc
    14581 agctatcgcg aagctgcccc gaaactaatt gagtatgtgc agcgattggg attcactcat
    14641 gtggagtttc tgcccctgat ggaccatccc ttctttggct cctggggcta tcaaaccacc
    14701 gggtactttg ccccctccgg caactatggg acgccgcagg acctcatgta cctgatcgac
    14761 cagctccacc agcacgatat cggagtcatt ctggattggg tgccgtccca ttttccgacc
    14821 gatgaacacg ggctcagtcg attcgacggc agccacctct tcgaacatgc ggacccccgc
    14881 cagggcctcc atcccgattg ggggacggcg gtgttcaact acagtcgtaa cgaagtccgc
    14941 agctttctta tcagcagcgc cctcttctgg ctggagcaat accatgccga cgggctgcga
    15001 gtggatgccg tcgcgtcgat gctgtatctg gattattccc gaaaagaagg cgagtggatt
    15061 cccaaccggc atggcggccg ggaaaacctg gaggccatca ccttcttacg gcaactcaac
    15121 gaagaaatct atcgccgcca tccggacgtg cagacctttg cggaagaatc cacctcctgg
    15181 ccgtccgtgt cacgcccgac gtatgcgggc ggattgggct tcggcatgaa atgggacatg
    15241 ggatggatgc atgacacgct ggagtacatg gcgctggacc ccgtgtaccg aaaacatcat
    15301 caccggaacc tcactttccg catgctctac gcgttccaag agaatttttt acttcccctg
    15361 tcccatgacg aagtcgtcca tggcaaaggc tcgctcctgg gcaaaatgcc cggcgacgat
    15421 tggcagaaat tcgccaacct ccgtgcgctg ttcggctata tgtacgccca ggctgcaaaa
    15481 aaactgattt tcatgggtgg tgaaatcggc cagtggcgtg aatgggcgca tgacgacagc
    15541 atcgattgga acctgctgca atacccgccc catcaaggct tgcaacgatg ggtcgccgac
    15601 ctcaaccatc tctaccgcgc agagccggcg ctgcatgaat tcgatttcga cccgcgaggg
    15661 ttcgagtgga tcgactgcca ggacgtggat gccggggtca tcagtctctt gcgacacggc
    15721 cggacaccac agcaacacat cgccgtcgtc tgtaatttta cgccagtccc acggctgcag
    15781 tatcgcgtcg gtgtcccaca aggcggctat tggaaagaac tgttgaacag cgacgcgtcc
    15841 atgtatggcg gcaccggcct tggaaatctc ggcggcctct cggctgagcc cattccggcc
    15901 cacgatcgaa cgcactcgtt ggcaatgacg ctgcccccgc tctctgtgct gttcttcaag
    15961 gccccctcct agcgccgtcc ctaggctcgc ctttccacgc ccagctacgg taggatcgcg
    16021 acacgagacg aaaaacatcg gacctcaaac gtccgattta ccgaggaggc gacgatggcg
    16081 attcgctcac gtacagcttc gatgtggcac gggctggtat tgctcacggc ctgcctcacc
    16141 ctcgcgaact gctcccacga catccacgag aaacgggccg acaccgttaa ggatcatgtc
    16201 gaagccttct acgatcactt gacacatgat cgcgtggcag cggccgtccg ggagaatgaa
    16261 gcgatcgaac acctctcatc ccagttaggc gacatcatct ctcggcgcgt caatcgtcca
    16321 ggaaccaacc aggtcgatcg cgaatggacc gaccttcgca ccgccaatga gacggccgcc
    16381 cagaactggc tggccttggg acaatatctc tcgatcaaga agcaatatgc gcaatcccga
    16441 gcaacctacc agcgtgtcat tgatacctac accggcacga cagagcggac ctatcgggag
    16501 caggcggcgc gagccattcg tgacttagac atcctcaccc cgcccccttc ctcccactaa
    16561 ccatggagtt cggaatgcgt cgccccacac ggggtctcaa gtaccgactc acaatcatgt
    16621 tgcttggcac ctatctggtc ggggccgggt gtgtgcatca tattcacgtc acctcaccat
    16681 cgagcgacaa ggctgcggcc ccgatccccg cgacggtgca gcttgaggta ccgtttctcg
    16741 ccatagaagg ggcggatcac atgccaggta ttcccctcct cgaatggccg tcgaaagact
    16801 tacaacaaac gattgtcgaa tatttcactc agcgccagac cttcacgtct atcggaacag
    16861 aagccggtga catgcgcctg gttgtgaaag cctggctcat gcttcgcgcg cctgaccgct
    16921 acctctaccg ggtccatttg gaatccgacc ttgccttttc cggacaagcc cccctcaaga
    16981 catacgcagc cgaaggtgaa gcccttggct cctcagtgag atgggttacc gcatcggatc
    17041 aagagccgat cgccgaggcc accgcccaag ccctgcacca attggccact cagattgaac
    17101 aagatcgcga gtttgtcatc aaggctactc accggtaaac tctcctgaca tatctgcttc
    17161 gagccacagc ctcgactcca cctcgactcg cgactcacgc acacatgtca catatgcacg
    17221 tatgctttcg gatacgaatc cgtatcccat ttgatacagg tctatcgtga gatagaccct
    17281 tatttcctct ctttcctcag gattcagcct gaaaaggcag tggttgagtt gttcacatgg
    17341 ctatcgatca cctgcatcga cacaattcca actcaccccg gcaccaaaca ataatggcat
    17401 taagtttgct ctcccatgag cgagcaatgc ggctacagcg ctgcttgggc ctgctctctt
    17461 cctatcgaag caagcggatc acaagaacag ctctaaccgt ttctcgtgcg cctaggagaa
    17521 agaagtcgcg tgtccaaacg gttgattctg tgcttcgatg gcatgtggga tttacctcca
    17581 gaccctggca ggcagttgcc ctcatcgagc actcccaaca cacaaaccct acataactcc
    17641 cacagtcatc tatctccggc cacgaatgta ctgcggctct accattcaat cctcccacgg
    17701 acggaggacg gacgtgcgca acagaaatgg tatgactccg gtgccgacat tccctggttt
    17761 tatcggttcc ggcctggctc atttgggtat ggagtggatc agaccatcct tcacgcttac
    17821 gcctacctcg ccgccaccta cgagcccggt gatgagctgt ttgtctatgg cttcagccga
    17881 ggtgcgtata ccgcacgttc tctcgtcggc ctgctcacca ccgccggcct cattcccgcc
    17941 gggcttctac ataccgatct tgtcgagcgc gtgcgtgagg tcgtctccca tccgtcacct
    18001 tcgactacaa catccactgc tcttcgacaa tgcctacatg agatgattct tgatccctcc
    18061 aaccaggccc tcgacgatgc ctatcgcctc tatcgtaagg gtcacggcga tatccttgca
    18121 ggaataccta cttccgcgag acggcgagga acactggaag ttccgatcac gctactcggg
    18181 ttgtgggaaa ccgtcggccc acttggaatt ccgaccaacg ccctcaaatg gctcaacgac
    18241 caccgctata attttcacga taccgaactg agttcgatcg taaaacaggc ctatcatgca
    18301 ctcgccatcg acgaacaccg cgcagatcac aacgcgacac tgtggacctc accggcctgc
    18361 ccggggcagt ccattgaaca acgctggttc gcaggggcac acggtgatct cggcggggcc
    18421 tacccggaac gggacttggc agatgtcgcc ttggcctggc tacagcggca ctcaatggaa
    18481 aagggattgg ctattgaaac ccgtttggtt ccgctgcagc caaatgcgct aagtccgatt
    18541 cacgattcgt ttagcgagtg ttttggaagg atccggaagt ggtttcattc acgattctat
    18601 cgaccagtca tgcagacggg aacgaatacc gaagtgctgg acagctccgt ccatacacgc
    18661 ctcatccaca cgccccatta tcgaccgaaa aacgagggat tgaaaagcgt cttacatttt
    18721 gaatgagccc tgttgtattc accaaatcta ccgacctccc gcgacacgac gccttaaaaa
    18781 gcaggacgac acagccttag aacgctacct gaccgaacaa caatccccac caagcacttc
    18841 gatgctcacc tccacagaca cacctactgc ccgttaattc gtactcttca atggcctatt
    18901 ttcctcaccc ctaggacgct cgccacaggt ccttaaggct ccgcgtcccc tgacgtgccc
    18961 ctcgttagaa gtaccctctt cgcagactat ctgatccgtc atagccgaga taggttcatc
    19021 accggagttt cgtcgtgctc gttcgccaca cgcgggaggt tcgcatgaca cgttcatcgc
    19081 atcgaagatt cgtcgttcac tgcatccttt ctgctgcggc catgggctgc gcagaaaaag
    19141 ggcggatcat cgagtccggt ctcgtcgggg cgccgcaccc aacctcacac agcctcatcg
    19201 acggagacta ccttgcgctc aaactgcagg ttcctgaaga tcgccgacca gtctcggtag
    19261 gtttggcgag agtcacctgg gcacaaggaa accgattcgg agtggaactc ctcatgatgg
    19321 atgctgatga acgggctcga ttgaatggtt tcctcgaaga acacctcccc ctcgaactgg
    19381 aatttcaaga ctcacaccag gaactcacga ttacagccgc ggaatagccc cacgtacaga
    19441 cggcaggaat agttctcttg aaaaaggtgg gcatgatgcc gagtggcttc catctccgcg
    19501 cctcccggcg agcgaagact cgatgcacct atcaatattt ggccaccagt aaaaacatcc
    19561 cacctgaggg taccaccaag gaggtacttc attcaggtct gtcggagatg accatccggt
    19621 tctacgctta cacataggca ctgaacaatg aaggaggcca ctatgacgtg ttctcgctgc
    19681 aacggatgca tggttgaaga tttcctgctg gatatggagg actcgtcggg gcccatgtgg
    19741 cttcaagcca aacgatgcat gaactgcgga aatgtggccg agaaggtgtt ggagcacaac
    19801 cgacagactc agggcgccgc actgcactcc gccaaatccg cgcccacgat cccaaacagt
    19861 gagcatgccc acttcggatc aggaggcatg gcacagctag ctgcctagtc tcaatgcaaa
    19921 gaccaggatg tcagggaaat cacggccgga ggcatcctgg tcacgattgt cttgcagtga
    19981 aatgtatgcg gataacgagc atccctcttc actactcact catgtctaga ccggtggaat
    20041 aaacaatgtc cattgatgta gagctcgctc tcattctggg gctgttccta atcggcgcgg
    20101 tcgtactcgc aattgataac ctggtgtatg cgatgtctcg acgtttgagc cgttggctgc
    20161 gcaaccgaaa atcactctct tctcccccgc ctctcagttc gacccagaaa aaacggctat
    20221 gacagcatgt cggctcgccc cgtcattcaa cctcgcaccc gcgaggaaca agagaccggt
    20281 tactgttggt cttttcctcc tctgcgttcg tttccaattg cacgttccgc cgagattcaa
    20341 aaggaggcgc aggtgaaacg tcgcccagcc gagagcatga aatgtgcgtc actaccgagc
    20401 actcgttcta ccggcatctc tcaaactctt ctctttttga tcatgaagca attggaagtc
    20461 agtcggccag gagacgatca tcaacagggg aggctgcagt gacctccacc gccaaaactg
    20521 tggtacgcga tgccgcgatc gtttacgggc tgacatttgc cgcggggctg tgcatggctg
    20581 cagccgggat aacccttgag agcaattctt caaccgcata tctctgtaat ctgttgtcag
    20641 gagtgctcgg cttcaccctg gtgggcactc gcctctccgc caaccgagcg gaacacttgg
    20701 catgggtggc ggcaaccctc tggaccttca accttacgaa tatcgtactg ggactccaaa
    20761 ccagctcagc atggatacac agcggcctca ccatcctgct catggcctcc ttaggtggaa
    20821 gcctcgccat gatcctcacc ctgacatcgg cagccgatcg tcgaacgtga atgtcagagg
    20881 tggctccggt tggttggaca gtgtagagca ttccttaagt ggtgcc
//
LOCUS       DAORQN010000017        20800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_178_length_20800_cov_74.072403, whole genome shotgun sequence.
ACCESSION   DAORQN010000017 DAORQN010000000
VERSION     DAORQN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20800
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_20800_cov_74.072403"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1161
                     /locus_tag="PK614_02440"
     CDS             304..1161
                     /locus_tag="PK614_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB81099.1"
                     /translation="MRLFSMTFVVSIILFVGVWSVRAAEPLSGADGQAGGAEAITGQD
                     GAPMVLVPAGPFTMGSNDGLPAERPEHVVTLDAYVIDRYEVSLQLYRKFLQAARYDAP
                     PTWDDEAAETVGDRPAVGMSWEDAAAYCAWAGKRLPTEAEWEKAARGTDGRRYPWGPM
                     QPFVDIANYNRGVWVSEAITLVSVAGGVEGMSVRHGLKAGGRSPYGLHHMAGNAAEWI
                     ADWYDRTYYGKSPDKNPTGPANGEKRVIRGGSWADLPVALSASARVSAEPGFQDRTIG
                     FRCAMDAKK"
     gene            1210..1731
                     /locus_tag="PK614_02445"
     CDS             1210..1731
                     /locus_tag="PK614_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81100.1"
                     /translation="MLDLGAASSVPPPNRKHKAVAAFAAGLVFLVALGGIRNISPSPD
                     STPAIQNIDPNMVPLVTGDEPIQELFVRAGCPVCHQIPGIAGAKGQVGPPLWLGKTGA
                     SRLADPQYKGQAQTVREYIVESVVSPGTYVVPGFPPDTMPTWYGRKLSGAALSKIASY
                     LEQAVEEPPSGRP"
     gene            complement(1814..2977)
                     /locus_tag="PK614_02450"
     CDS             complement(1814..2977)
                     /locus_tag="PK614_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRB81101.1"
                     /translation="MDVRTLLEVMVKQESSDLYLTVDAPPIYRIHGSTHRTDAPPFTN
                     EQLESLALALMRGQQRGEFEEKMEMNLALYYKDLGRFRVNIFRQKGNVGLVFRHIKAE
                     IMTVEQLDLPPIVKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTVHAGHIISVED
                     PIEFVHHHKKSIVTQREVGFDTHSFGHALKNTLRQAPDVILIGEIRDTETMEAAITFA
                     ETGHLCLGTLHSNNANQSIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSLDGRR
                     VPALEIMLDTPRIKDLIKRSEIDTLKEAMEQGIDEGCQTFDHVLLQLYKAGKISIEQA
                     LINADSANNLRLKIKLAGLKGDDAVAALLDKNDRDEKGFQIQGQLGQGGGKKR"
     gene            complement(3034..4095)
                     /locus_tag="PK614_02455"
     CDS             complement(3034..4095)
                     /locus_tag="PK614_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRB81102.1"
                     /translation="MIDISKLLTFGVQQGASDCHISAGEPPMIRLHGDLKKLDHPPLT
                     QDETHALIYDMMSDAQRKNFEEHRECDFSFDLGDIARFRVNVFVQGRGLGAVFRTIPT
                     EILPLEKLGMPPILRQLCDREKGLILVTGPTGSGKSTTLAGMIDYLNNTFEGHILTIE
                     DPVEFVHKSKKCLVNQRELGVHTLSFANALRAALREDPDIILVGEMRDLDTIQLALTA
                     AETGHLVFATLHTSSAPKTIDRIIDAFPPNQQAQVRAQLSETLEAVLTQTLLKKKSGG
                     RIAAVEIMVGTTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVDLVTRGLVTKAE
                     AQSRSMNPNLFSAAAGGAA"
     gene            complement(4274..5434)
                     /gene="bioF"
                     /locus_tag="PK614_02460"
     CDS             complement(4274..5434)
                     /gene="bioF"
                     /locus_tag="PK614_02460"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRB81103.1"
                     /translation="MFKEYLQKLQDQHLLRRLRTIASSTGPTVRLDGHTVILLSSNNY
                     LGLATHPAVVEAAVEATRQYGAGSGASRLVCGTLTPHEELETALARFKDTEATLTFAA
                     GYLANISAVPALIGKDGLILADRLCHASLIDGCRLSGATFRIYHHRDMNHLEQLLGRR
                     PPGKPTLIVTDGIFSMDGDIAPMRDIALLAEQYGAAVYVDDAHGTGILGETGRGTLEH
                     CDVETRIPYHMGTLSKAIGSSGGYLAGSAEFVAYLVNTCRAFTYTTAPTPAAAAAATA
                     ALRVIRREPERRARLWRNRHRLAQGLSGLGFLLAASESPILPVIVGDPDRAMNLAHTL
                     LTLGVYAPAIRPPTVPASTSRIRFTITADHTDEHIDQALTALAQAGRTLNMI"
     gene            5556..6389
                     /locus_tag="PK614_02465"
     CDS             5556..6389
                     /locus_tag="PK614_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81104.1"
                     /translation="MSYRIKVPAKVDPLDEAHLLTGVERFLLVLQEQRRAVLVGLGVL
                     LVAAAVVAGVIWYDYQATLKARELDQEATLHYLNRPADDPKKSHEQLAQAINLYKQVV
                     DQYPRSPVAPLALFHLGNAQVLANEVDAGIETYKRFMLLHGSNTSLLGLVQQRMAYAY
                     LVKGDRDQAVKAFTGILEIPGALNKDHVLFELAKLEESQSRPEGALAHYQDLMKNYPN
                     SPFTSEAAVRVKVLEVKKSPESPAAAATPAPAVTAPAPQPEAPAKSSGKSAAASSKKK
                     P"
     gene            complement(6430..7821)
                     /locus_tag="PK614_02470"
     CDS             complement(6430..7821)
                     /locus_tag="PK614_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB81105.1"
                     /translation="MHARFLGTGVRALPVLSFCLVSLAWPALAGAESPTPSHADTPAT
                     TETTPEDIQPSIQEPEYSLDEEDHATAAELEAVEPPAPIASEPSTEVPPAEALLQLKP
                     LNGTTARFTDLLTPPAEVVQEEASAQDQESDEATAYNVPIVLDPSVQGHIRFFNVSIR
                     NRFEQWLIRLSHYRPLVDSIFSEFQLPSDLIYLSLVESGFNPHAYSRARAAGPWQFMK
                     GTAKVYGLRVDSYVDERRDPVKSTVAAARYLRDLYDLFGTWPLAMAAYNAGEGKVMRA
                     LHTAQAESFSDIAKTRLIRRETKEYVPRFMAATIIAKNPDRYGFPQNDVRPHQFEEVV
                     VKRPIHFKAIANVTGISYQELKVLNPELRRDATPPDDAEYHLKVPVGTREKVEQLLDR
                     APTHKFAPLPVPVRVRHVKPEPESGHWYRVRVGDSLERIAKRFNISVKTLKSNNNLTG
                     PTIKVGSRLVIAN"
     gene            8127..9272
                     /locus_tag="PK614_02475"
     CDS             8127..9272
                     /locus_tag="PK614_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRB81106.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDKLFAEVRED
                     LKWLFQTRNDVLILASSGTGGMEGSVSNFLSPGDKALTINGGKFGERWTKLCKTFGAQ
                     VTELKVEWGRAVDPQAVADALKKDPSIKAVYVQASETSTAVAHDVKALADIVKGYEET
                     ILVVDAITALGVLDLKTDAWGLDVVITGSQKALMLPPGLAFASVSEKAWRLADKAKNA
                     AFYFNFKRERENQQKSTTAYTPAVSLILGLKEVMNMLKAEGLEAVFARHAMLATAMRE
                     GVKAAGLSIFPQERPSDALTAISAPEGVDGQAVYKNLRTQYGMTAAGGQDHLKGKIFR
                     ISHMGYIDSFDVITALAAVEMVIKGLGYPIKLGSGVAKAQEIIMGKS"
     gene            9301..10893
                     /gene="serA"
                     /locus_tag="PK614_02480"
     CDS             9301..10893
                     /gene="serA"
                     /locus_tag="PK614_02480"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRB81107.1"
                     /translation="MKILVSDSLSKQGVEVLEKAGFTVVVKTKLPKEELLKEIKDADG
                     LIVRSGTKVTAEVIAAASQLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMAMIFSMSRRIPQATASTKGGKWEKEKFMGVELYNKVLGIVGVGQIGGYLTKLAQG
                     VGMQVMAYDPYLAPERAEKMGVTIVELDELFRRADVISVHTPLTPETKALINAQAIAK
                     MKTGVMIANCARGGIVHEGDLCEALKSKKVAAAAFDVFEDEPVKPDNPLLALDNFICS
                     PHIGASTTEAQENVAIGIAEQIVEYFTKGIARGAVNIPSVSPELLPKLQPYLSLGERV
                     GLLQAQLLEGGLERLTVEYSGEVAGLNVAPLTIAVLKGLLTPILEDPVNYVNAPVVAK
                     ERGIEVKEVKISDAGDFASVIRVRVEAGKKSHQVAGTLYHRKDPRIIEIDQFKVEVVP
                     DNHLLLIQNEDRPGVIGTVGHILGDHNINIARMQCSREERGGKALQIFGLDAPLPKSV
                     LDQITNSKHILSVKVADLSKGL"
     gene            10912..12036
                     /gene="hisZ"
                     /locus_tag="PK614_02485"
     CDS             10912..12036
                     /gene="hisZ"
                     /locus_tag="PK614_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRB81108.1"
                     /translation="MGALSLKASSSSSPLVAGRERSLIPVGMSTILPDAARRIRQLEQ
                     TLLAVLARAGYEEIILPMFEYLDVLAPGLESELIEKCYQLVDRTTGRLMLLRPDATAQ
                     IARTVAMGMMGERLPLRLCYRTSVFRYEREHAGRDREIFQVGAELIGVDGVTGDAEVL
                     TLLLECLAQVGLSSFKVAVGHVGFFTALLVKAGLSQDGQKRVEQAAARKDMPLLEELL
                     TREGVSRSTGRVILEALELCGGPEVLAHGRKLVGRDRTLLRPLDRLAQVYERLVSPGQ
                     QAVLLDLGEFRGFEYYDGIVFDVFAPGIGAELGGGGRYDHLMGRFGRTAASTGFGLDV
                     DRLFRALDASGVVTPPHSSPSTTAGSKPVAAEPRRRRSRV"
     gene            12033..13388
                     /gene="dnaB"
                     /locus_tag="PK614_02490"
     CDS             12033..13388
                     /gene="dnaB"
                     /locus_tag="PK614_02490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRB81109.1"
                     /translation="MKSLSAVDLTSPKLPPQNVEAEQSVLGAILLDNTAMAKVMELLT
                     DEEFYRTAHRKIYQAMLELSDRGEVIDQITLTECLKARSELEAVGGSAYLAELVQVVP
                     TAANVRYHSKIVRDKALLRGLIQTSTEVVTRGYDGTVAVDELLDFAERSVFSLAQGKL
                     DRSFTPVNQIIKESLDVVDKLSKRKERVTGVPTGFIDLDDLTAGLQPSDLIVIAGRPS
                     MGKTSLALGMAQHAALHAGTVVGIFSLEMSKPQLVLRMLSSEARVDSHALRTGRLQKE
                     DWWRLAEAAGKLEQAPIYIDDSGAVTVQQMRGKARRLKAERGLDLLIVDYLQLMQGKS
                     DSESRQQEISDISRSLKTLAKELNVPVIALSQLSRAVEARKPPVPMLADLRESGAIEQ
                     DADVVMFIYREEVYEPATERKGIAEILVSKHRNGPIGKRDLFFHDRFAKFENLESREV
                     V"
     gene            13555..15333
                     /locus_tag="PK614_02495"
     CDS             13555..15333
                     /locus_tag="PK614_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81110.1"
                     /translation="MACVTAACQSTPPARPAPAAVPSAVPVAPRAMPSSSDSRASYHF
                     LLGYQAELDQETELAIKEYQLALQTDPASNYLKARLAVLYFTAGDVPSAVRFADEVAD
                     VPGLDAQMLGQIGGMYAAAGKPDKALRLFNRAIEQEPQRSEHFFAKGLLQANQKQYVE
                     AEDTIRAGIKISPDSAVGYYYLGRIGVEARDFDKATTHFEQAVALNPAFEPAYVALGS
                     VYEAKQDRDKAIGIYRRYLQGVNPKNRDIRHHLIRLQVSAKQYDEALRELQEMLSEDP
                     SDLDAQLRMGLVYGEQKDYPKAIQQLKQILTVRPTELKVRDYLGYLYEETKDYANAME
                     AYRYNLTLEPSYFEGHLHLGVLQYRTKQYVESIQHLREASRLNPKQPEAHIVLGLSHF
                     QVEQYEPSLQAFQEGIRHNPDNADLHFNAGTAYDKLNRFDDVVKSMQTTLALDPHHAD
                     AMNYLGYSYAERGVKIEEAIALTKQAVALRPTNGYYVDSLAWAFFKKGLLAEALAEMK
                     RAVALVGDDPVIYEHLGEIYLKQQHLSDGREALLHSLELDPSNDKLMQRFRELGLGDP
                     AKEERIRQALRRVSDRKGATPAPAQQ"
     gene            15730..16257
                     /locus_tag="PK614_02500"
     CDS             15730..16257
                     /locus_tag="PK614_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRB81111.1"
                     /translation="MVTTSKQQGFTLIELMVVVVIVGILAAFAIPNFLRYRAQAMQAE
                     ARSNLAGIFVAETSFFSERKEYGNFTDVGFAIAEGGTNRYTYRSGLGIGSGMGPNGGN
                     LCGSSGSCDTIQTASPMAGAMTYTGAVGSATTSASGFTATAAADLDGDATHDGWYVND
                     AKQGLNGAEPNDVSS"
     gene            16272..16427
                     /locus_tag="PK614_02505"
     CDS             16272..16427
                     /locus_tag="PK614_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81112.1"
                     /translation="MSKDDGKWNGVQAAGPEVEGIMDLNNWKIEEMIRNYRKEVLGIE
                     AAIPKIC"
     gene            16508..17026
                     /locus_tag="PK614_02510"
     CDS             16508..17026
                     /locus_tag="PK614_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB81113.1"
                     /translation="MLKQMKGQKGFTLIELMIVVAIIGILAAIAIPNFLRYQAKSRQS
                     EAKTNLGAIFVAETAYLSEQGRYGSFSEIGYALAGATNRYTYRSPAALGGGASSDTAL
                     IDYIACGAPAACVNPADGATGSFASLAVVSPPTFTAAAVANLDSDGTIDGWSVNDIKG
                     GLTQAVPDDVTS"
     gene            17245..19242
                     /locus_tag="PK614_02515"
     CDS             17245..19242
                     /locus_tag="PK614_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB81114.1"
                     /translation="MKILPSMSLLMISALVIFSPLQEGGTTHPAQMIIRLLILAWLGI
                     VLATGIRAGRLAVPVLAMRYVVLAFVGLALVATLFSPYAHSSRQWLIMIMGYVTIFYL
                     LVSCVDRWEHVRTLTVMIALMGIGEAGWAIVQGLAWNVLRPGGTFFNPNFLAGYLTVT
                     WTILLSVAIYGYRPVSTGSRPWMSPVLWWLGLGTALGGLFLAILLTQSRGGMIVCLVG
                     TVFVLTARYGWKLAGSCVAAVVLLGLVVPTPIRDRVLAEHQQNPVSYARWQMWQGAVA
                     QMVDHPLGIGLGLYQYTYPQYAFPVDGEIARFGKVAQTPHNDYLQLGVEMGPGAILVF
                     GSGVVLLFRELRHVLHARLRRWQRSLIVGVGGGGIALLVHAALDSSLRESALAILLVL
                     CGAVIVSAARLTRRGTDAVYVVPIHSRWAWGIGAACLVLVAGAEVTRMGVAWIKFERA
                     SRQAMAGETVAAIEGLKAAVALDPGKALYHHGLGSVYARAFESSREKQVFQLAYAEFK
                     QAIELNPLDSRLLGLLGQLYVSAAQTSSSPAAFDAQQKAWLRAAVQVYERAIQLAPFS
                     AMYRYEQAQLHWMLGERSEAEQRAKEAENLEPNFLPARALLARLWVDEGRVDQARGQL
                     REILARQARYKEWSKNGLDQAFLNVDVAPLRAAVGEKAVAG"
     gene            19239..20189
                     /locus_tag="PK614_02520"
     CDS             19239..20189
                     /locus_tag="PK614_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81115.1"
                     /translation="MKGTSMKQGLIHAALGVILLAGSSVLLHLLDGQRTAVARAEQLA
                     YLPKGDYLKLAVLGYRQVVADLIWLQAVQHIGAKRDTQAGYMWTYHAVDVLTDLDPTF
                     VPPYQATGLFLGVLVGRHEEGVAILKKGIQHNPSHWQLPFLAGYISYYERCDPAAGGE
                     FFRIAARVPGAPAYLPQLAARMTVESGDSSAALEFLERFSRSVTDERVREALFRRMKE
                     IVQEQDLRVLEEGISRYRAHYGQAPAKLEDLLLRGIITSLPMDPLGGQYELNSLTGTV
                     SASSTRERLRIHEKVACRLGRATQSEGGAGSMETPLPTVQ"
     gene            20205..>20800
                     /locus_tag="PK614_02525"
     CDS             20205..>20800
                     /locus_tag="PK614_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81116.1"
                     /translation="MDDIVTDNLTKSYASGWPGRPPFVALDGLSLTVRRGEIFGFLGP
                     NGAGKTTTLKILLGLVRATSGQAYLLGQPAGDVAARRRIGFLPESPYFYDYLTAEEFL
                     GFYGQLAGLARSAITQRVTDLLGLVGLTDARTRQLRKFSKGMLQRVGLAQALIHDPEL
                     IILDEPMTGLDPVGRKQVRDLILNLRDRGKTVFFSTHIL"
BASE COUNT         4126 a         5478 c         6635 g         4561 t
ORIGIN      
        1 ctaagcacca cgccgggggg gttgcgtacc taaagaaatg aaccagttat agctttgcgc
       61 cgggtgtccc gttcggccgg catgcgtcgc aggccggccg aatctgtttc agcatcttcc
      121 ccccgtagct gttccacgaa gtgtcgggat agattgcagg gcttgctcgt ctccgccagt
      181 tgagtaaatc cctctccagt gcacgatcct ttacatgtag ttgagtttgc acggactcgc
      241 ggctacactt cgactgggta gagtcgggtt gtgtctaggt gtatccgtcg gtgagaggtg
      301 tatatgcgtc tgttcagtat gaccttcgtg gtatccatca ttctgtttgt cggagtctgg
      361 tcggtgcgag cggccgagcc actgtctggg gcggatggcc aggcgggcgg ggctgaggcg
      421 atcacagggc aggatggtgc gccgatggtg ttggttccgg ccgggccttt tacgatgggg
      481 agcaacgatg ggcttcccgc cgagcgcccc gaacatgtgg tgaccctcga cgcgtatgtc
      541 atcgatcgat atgaagtgtc gctgcaactc tatcgaaagt ttcttcaggc ggcgagatat
      601 gatgcgccgc cgacctggga tgatgaagcg gcggagacgg tcggcgatcg tccggctgtc
      661 gggatgagtt gggaggatgc ggcggcctat tgtgcctggg cagggaagcg actcccgacg
      721 gaagctgaat gggagaaggc tgcgcgtgga accgatggcc gtcggtaccc gtggggcccc
      781 atgcagccgt tcgtggacat tgccaactac aatcgcggcg tgtgggtgag cgaagccatt
      841 acgttggtga gtgtggccgg tggggtggaa gggatgagtg tccgccacgg gctgaaagcc
      901 ggggggcgga gtccgtacgg cctccatcac atggcgggga atgcggccga gtggatagcc
      961 gattggtacg atcgtacgta ttacggcaag agtcccgaca aaaatccgac gggtcctgct
     1021 aatggcgaga aacgggtgat tcgtggcggg tcgtgggccg atttgcctgt ggcgttaagt
     1081 gcgtctgcgc gggtctcggc ggagcctggt tttcaggacc gcaccattgg atttcgctgc
     1141 gcgatggatg ccaagaaata gagccagggg tgagctctga gaccttcgta agtttcctgg
     1201 ttcctgccta tgcttgattt aggtgccgca tcgtctgttc caccgccgaa ccgaaagcac
     1261 aaggccgttg ccgcgttcgc ggcagggctt gtgtttctgg ttgcgctggg ggggatccgc
     1321 aatatctcgc cctcgcctga ttcaactccg gctatccaga atattgatcc gaacatggtg
     1381 ccgttggtga cgggggatga accgattcag gaactctttg tccgcgccgg ctgtccggtt
     1441 tgccatcaga ttcccggcat tgcaggagcg aaggggcagg tcggtccacc actgtggctg
     1501 gggaagaccg gggcgagtcg cctggctgat ccacagtata aggggcaggc gcagacggtt
     1561 cgtgagtaca ttgtggaatc cgtggtgtcc ccagggacct atgtggtgcc cgggtttccg
     1621 ccggatacga tgccgacgtg gtacggacgg aagctgagtg gggctgcatt gagtaaaatc
     1681 gcgtcgtatc tcgaacaggc ggtagaagag cccccctcgg gacgccctta agcgggtgtg
     1741 ctgatcaatt gcgtggtgtg cgctcgcgcc ggaataacct ccagcagatc aggactcatc
     1801 cacgccctgc ccctcaccgt tttttgcccc cgccctgacc cagttgccct tgaatctgga
     1861 accccttctc atcccggtcg ttcttgtcca ggagggcggc caccgcgtca tcgcctttca
     1921 atcccgccag tttaattttc agacggaggt tgttggcgct gtcggcattg atcaacgcct
     1981 gttcaatgct gatcttccct gccttataga gttgcaggag cacgtggtcg aatgtctggc
     2041 agccttcgtc gatgccctgt tccatcgcct ctttcagggt atcgatctca gaacgtttga
     2101 tgaggtcctt gatgcgcggt gtatccagca tgatttcgag tgcaggcacg cggcggccgt
     2161 cgagggaggg gatcaatcgt tgagagatga tggcccgtag gttcaatgag agctgcagat
     2221 agatctgcgc gtgccgttcg accgggaaga agttcatgat gcgctcgatc gattgattcg
     2281 cattattcga gtgcaacgtt ccgaggcaga ggtgtccggt ttcagcgaac gtgatcgcgg
     2341 cttccatggt ttccgtatcc cggatttcac cgatgagaat gacgtccggt gcctgacgca
     2401 gggtattttt caacgcgtgt ccgaaggaat gggtgtcgaa gccgacttcg cgctgggtga
     2461 cgatcgattt cttgtggtga tggacaaatt cgatcgggtc ttccacgctg atgatgtggc
     2521 ccgcatgcac cgtgttgcgg tggtcgatca tcgcggcgag ggacgtggat tttccggagc
     2581 ctgtcgcgcc gaccaccaac accagacccc gcttggtcat agcgatgtct ttgacgatgg
     2641 gcggcaggtc tagctgttcg acggtcatga tctccgcttt gatatgccga aacaccagcc
     2701 cgacgttgcc cttctgtcgg aagatattga cgcgaaagcg gccgaggtct ttgtaataaa
     2761 gggcaaggtt catctccatc ttttcttcga actcacctcg ctgctggcct cgcatgaggg
     2821 ccagcgccag cgattcgagt tgttcgttcg tgaacggcgg cgcatcggtc cggtgtgtcg
     2881 agccatggat gcgataaatg ggcggcgcat cgacggtgag atacaggtcc gacgattcct
     2941 gcttgaccat cacttcgaga agcgtgcgaa catccatgaa atgcctcttt ccaatgccgt
     3001 gggaaacccg tttcgtttgc gccgagtgcc cgtttacgcg gcgcctcctg cagcggcgct
     3061 aaagagattg ggattcatgc tgcgcgattg agcttcagct tttgtcacca gccctcgtgt
     3121 gaccagatcg accagggcca tgtccatggt ttgcatccca tctttttggc tggcctgcat
     3181 gatgccggga atttgatgga gtttgccttc gcggatgagg ttccgcaccg ctgtggtacc
     3241 gaccatgatt tccaccgccg caatccggcc accactcttc ttcttcagga gcgtctgggt
     3301 caagactgcc tccagcgttt ccgagagctg tgcgcggact tgggcctgtt ggttcggagg
     3361 gaaggcgtcg atgatgcggt cgatggtttt cggggcgctg gacgtgtgca gggtcgcaaa
     3421 gaccaagtgc ccggtttcgg cagcggtcaa cgccagctgg atcgtatcca ggtctcgcat
     3481 ttcgccgacg agaatgatgt cggggtcttc gcggagtgcg gcgcggaggg cgttggcgaa
     3541 cgacagcgtg tggacgccca gttcccgctg gttgaccagg cattttttgg atttgtgaac
     3601 aaactcgacc ggatcttcga tcgtgagaat gtgtccttcg aaggtgttgt tgagatagtc
     3661 gatcatgccc gcgagtgtgg tggatttccc ggacccggtt gggccggtca ccaggatcag
     3721 gcctttttcg cggtcgcaga gctgtcggag gatcggcggc atgccgagtt tttccagggg
     3781 aagaatctcc gtcggaatgg tccggaacac ggcgcctaag ccgcgccctt gcacaaagac
     3841 gttgacgcgg aatcgggcga tgtcgcccag gtcgaaggag aagtcacact cccggtgttc
     3901 ttcgaaattc ttccgttgcg cgtcactcat catgtcgtag ataagcgcgt gagtttcatc
     3961 ttgggtcagg ggaggatggt cgagtttctt gagatcgcca tgcaggcgga tcatcggtgg
     4021 ttcaccggcg ctgatgtggc agtccgaggc gccctgttgg acgccaaagg tcaacagttt
     4081 ggagatatca atcatcggat cactccatgc agtgagagaa tgtatggata aacgagcgtc
     4141 gctatgagta gaggatcatg gctcgtcagg ttgattcgat catgccatag gaaggtgaga
     4201 aagcaagaaa atacccagaa gagacgaggg agaaacggtg gctggcagcc gctctgcaaa
     4261 gggcgattgg tgcttagatc atgttgagcg tgcgacctgc ctgtgcgagt gccgtcagcg
     4321 cctggtcgat gtgctcatcg gtgtgatcgg cggtgatcgt gaagcgaatc cggctcgtgg
     4381 acgccggcac cgtcggtggg cgaatggcgg gggcatagac tcccagtgtg agcagtgtgt
     4441 gggcgaggtt cattgcgcga tcgggatcgc cgacgatgac cggaagaatg gggctctccg
     4501 aagcggcgag caggaagccg aggccgctga gtccttgcgc aaggcgatgg cgattccgcc
     4561 acaatctggc tcgccgctca ggctctcgcc ggatcacgcg gagcgccgcc gtggcggccg
     4621 cggcggcggc cggcgtgggg gcggttgtat aggtgaaggc gcggcaggta tttacgagat
     4681 aggccacgaa ttccgctgat ccagccaggt aacctccgga gctcccgatc gctttgctca
     4741 aggtgcccat gtgatagggg atgcgcgttt cgacatcgca atgttcgagg gtgcctcgcc
     4801 ctgtttcccc aaggattccc gtgccgtgcg catcgtcaac atagacggcg gcaccatact
     4861 gttccgccaa tagggcgatg tccctcatcg gtgcgatatc accatccata ctgaaaatcc
     4921 catcggtgac gatgagggtc ggtttgccgg ggggccggcg gccaagcagt tgctccaggt
     4981 gattcatatc ccggtggtgg tagatgcgaa atgtggcgcc gctcaagcgg cagccgtcga
     5041 tcagacttgc gtggcagagg cggtcggcga ggatgagccc gtcttttcca atcagcgccg
     5101 gaacggcgct gatattcgcc agatagccgg ctgcgaaggt gagtgtggct tcagtgtctt
     5161 taaaccgggc gagtgccgtt tcgagttctt cgtggggcgt aagggtgccg cagacgagac
     5221 gcgaggcccc ggatcccgcc ccgtattgac gcgtcgcttc gacggccgct tctacgacgg
     5281 ctgggtgggt ggccaggccc aggtaattat tggaggaaag caggatcacg gtgtgcccgt
     5341 cgagcctgac cgtcggcccc gtggaggagg cgatggtgcg cagccgacgg agtaggtgct
     5401 ggtcctgcaa tttttgaaga tattccttga acatggtgtc ccgtactcgc gaagggcctg
     5461 aatatttgac aaggctgact gctccctagt ataaggcgcg aggcgctcgg aaacgaccgc
     5521 ttcttttact gcgcctagag aatgattgag agagcatgag ttatcgaatc aaggttcccg
     5581 caaaggtcga tccgctcgat gaagctcatc tgctgaccgg agtggagcgg tttctgcttg
     5641 tcttgcagga acagcggcgg gcagttctcg ttgggctggg agtcttgctc gttgccgcgg
     5701 cggtggttgc cggggtcatt tggtatgact accaggccac gctgaaagca cgtgaactcg
     5761 atcaggaagc gacgctgcac tatctcaatc gaccggctga cgatccgaaa aaatctcacg
     5821 aacagcttgc gcaggccatc aatctctaca agcaggtagt ggatcaatat ccacggagcc
     5881 ccgttgctcc cctggccctg ttccatttag ggaatgctca ggtgctggct aatgaggttg
     5941 atgcagggat cgaaacctac aaacgattca tgttgcttca tggttccaat acctcgctcc
     6001 tggggcttgt ccagcagcgg atggcctacg cctatctggt gaagggggat cgtgatcagg
     6061 cggtgaaggc gtttaccggc atccttgaga tccccggtgc gctgaataaa gaccacgtgc
     6121 tcttcgagct ggcaaaattg gaagaatcgc agtcccgtcc tgagggagcc ctcgcgcact
     6181 accaggattt gatgaagaat tatcctaact cgcccttcac cagtgaagcg gccgtgcgag
     6241 tgaaggtgct ggaagtgaag aagtctccgg agagtccggc ggcggctgct acacctgctc
     6301 cggccgtgac tgcgcccgct ccccagcctg aggcgcctgc taagtcttca ggcaagtctg
     6361 ccgcggcgtc ctccaagaaa aaaccgtaac ggctccctta gtagaatgac gcggtgaggg
     6421 gtggtcgcgt tagttcgcga tgaccaggcg gcttcctacc ttgatggtgg gacctgtcag
     6481 gttattgttg gacttgaggg ttttgacgga gatgttgaat cgcttggcga ttctctctag
     6541 agagtcgcct acgcgcaccc gataccagtg gccggattcc ggctcaggct tgacgtgccg
     6601 gaccctgacg gggaccggga gcggcgcgaa tttgtgagtc ggcgctcgat ccaacaattg
     6661 ttcgaccttc tccctggtgc cgaccggaac cttcaggtga tattcggcat cgtccggggg
     6721 cgtggcatcg cgccgtaact ccgggttcag taccttcagt tcctggtacg aaatgcccgt
     6781 cacattggca atcgctttaa agtggatggg gcgcttcacg accacttctt caaactggtg
     6841 aggccgcacg tcgttctgcg ggaagccata gcggtccgga ttcttggcga tgatggttgc
     6901 ggccatgaat cgggggacgt attctttcgt ttcccgccgg atcagccgtg tttttgcaat
     6961 gtctgaaaag ctctccgctt gcgccgtatg gagggctcgc atcaccttgc cctcaccggc
     7021 gttgtaggcg gccatggcca aaggccaggt gccgaacaaa tcgtacaggt cccgcaggta
     7081 gcgcgcagcc gcgacggtcg acttcacagg gtctcgccgt tcatccacat aactatccac
     7141 acgcagccca tagactttgg cggtcccttt cataaattgc catggaccgg ccgcacgggc
     7201 gcgggaataa gcgtgggggt taaatccgct ttcaaccaga gacagataga tgagatcgct
     7261 ggggagctgg aattcagaga agatgctgtc gaccagcggg cgatagtgac tcaggcggat
     7321 gagccactgt tcaaatcgat tgcggataga gacgttgaaa aaacgaatat ggccttgaac
     7381 ggatgggtcg agcacaatcg gcacgttgta ggccgtggct tcatccgatt cctggtcctg
     7441 cgcagaagcc tcttcctgaa ccacctctgc gggtggtgtc agcagatccg tgaatcgagc
     7501 ggtggtgcca ttgaggggct tgagttgaag gagcgcttcg gccggcggga cttcggtcga
     7561 cggttcgctg gcaatggggg ccgggggctc aacggcttcg agttccgcgg cagtggcgtg
     7621 gtcttcttcg tccaacgagt attccggttc ctgaatggat ggttgaatgt cttccggcgt
     7681 agtctccgta gtcgcgggtg tgtcggcgtg ggatggggtg ggcgactccg ctcctgcgag
     7741 ggccggccac gcgagagaga ccaggcagaa cgagagaacc ggcagagcgc gaacacctgt
     7801 gcccaggaaa cgtgcatgca taagggtacc catcagactg ttcaggaaat gtgagaatgc
     7861 tgactctcga agggtagcct atcgaggagg gtacaacttg tcaagaaatt gagggagttg
     7921 agtcgtcatc gcgtttacag ggagaaaaca gtgcgcagga gatcgcgctg cgtctctgaa
     7981 agaaatcttg cgcgatgcag tgaagtcgct gaaaagataa gcatcttcac ggttcgttac
     8041 cttgacacct cagaggggct atggtagcgt acgccccttc taaatccctg acatcgttcg
     8101 atctgcacag tgaggaggtt cgtcccatgc tcaaacggta tttgctagct cccggtccca
     8161 cccccgttcc tccggaggtg ttactggcca tggccagacc catgattcat caccgggcgc
     8221 ccgaattcga taaactgttt gccgaggtcc gtgaggacct gaagtggctg tttcagacca
     8281 ggaacgatgt gttgattctc gcgtcttccg gcaccggagg gatggagggc tcggtttcga
     8341 atttcttgtc cccgggggat aaggccctca cgatcaacgg cggcaagttc ggcgagcgtt
     8401 ggacgaagct gtgtaagacg ttcggagcgc aggtcactga gttaaaggtc gagtggggcc
     8461 gtgcggtgga tccgcaggcc gtcgccgatg ccttgaagaa ggatccgtcg atcaaggccg
     8521 tctatgtgca ggcgagcgag acctccacgg ctgtggcgca tgacgtcaaa gccttggccg
     8581 atatcgtcaa agggtacgag gagacgattt tggtcgtgga tgcgattacc gccctgggag
     8641 tcctcgatct caagaccgat gcctggggcc tcgacgtagt catcaccggg tcgcagaaag
     8701 ccttaatgtt gccgccgggc ttagcctttg ccagcgtgag tgaaaaggcc tggcgattgg
     8761 ccgacaaagc gaagaacgcg gcgttctatt tcaattttaa gcgcgagcgt gagaatcagc
     8821 agaagagcac gacggcctat acgccggctg tgtccctgat tctgggcctc aaagaagtaa
     8881 tgaacatgtt gaaagccgag gggctagaag ccgtctttgc ccgtcacgcg atgttggcga
     8941 cggcgatgcg agaaggcgtc aaggccgcag ggctctccat cttccctcaa gagcgtccga
     9001 gcgacgcgtt aaccgcgatt tcggcgcctg aaggtgtgga cggacaggcc gtctataaga
     9061 atttgcggac ccaatatgga atgacggcgg cgggtggcca ggatcacctg aagggcaaaa
     9121 tcttccgtat ttctcacatg gggtatatcg atagttttga cgtcatcacg gccctggcgg
     9181 cggtggagat ggtcatcaag gggctgggct atcccataaa actcggtagc ggggtggcaa
     9241 aggctcaaga aattattatg ggcaagtctt agactcaccg tcaggtaagg cgtcacgaca
     9301 atgaaaattt tggtcagcga tagtctctcg aagcagggtg tcgaggtatt ggagaaggcc
     9361 ggctttacgg tggtggtgaa aaccaaactg ccgaaagaag agctgctgaa ggaaatcaag
     9421 gacgcggacg gactgatcgt ccgctccggg acgaaagtca ccgcggaagt catcgcggcc
     9481 gccagccagc tgaaggtcgt cggtcgagcc ggttccggct tggacaatgt cgataccccg
     9541 gccgccacgc gccgcggcat cgtcgtcatg aatacacccg gcggcaacac cgtgacgact
     9601 gccgagcata cgatggccat gattttctcg atgtcgcgcc gcatcccgca agctaccgcc
     9661 tcgacaaagg gtgggaagtg ggaaaaagaa aagttcatgg gcgtggagtt gtacaataaa
     9721 gtgctcggca ttgtcggcgt cggccaaatc ggcgggtatc tcaccaagct tgcacaggga
     9781 gtggggatgc aggtgatggc ctacgatccc tatctggccc cggagcgtgc ggaaaaaatg
     9841 ggtgtgacca tcgtcgagtt ggacgagctg ttccgccgag ccgatgtcat ttccgtgcat
     9901 actccgctga ccccggaaac caaagccctg atcaatgcgc aagccattgc caagatgaaa
     9961 accggcgtca tgattgccaa ctgtgcgcgt ggtggaatcg tgcacgaggg agatttgtgc
    10021 gaggcgctca aatccaagaa ggttgccgcc gcggcattcg acgtgttcga agacgagccg
    10081 gtcaagccgg acaatccgct cctggcgttg gataatttta tctgctcgcc gcacatcggc
    10141 gcctccacta cggaagcgca ggaaaatgtg gcgatcggca ttgccgagca gatcgtggaa
    10201 tacttcacca aaggcattgc ccgaggtgcg gtcaatattc cctcggtctc accggagctc
    10261 ttaccgaaac tccagccgta tctttcgctt ggggagcgtg tcggcctctt gcaggcgcaa
    10321 ttgttggaag gcgggctgga gcggttgacc gtcgagtata gcggagaagt ggcgggtttg
    10381 aacgtggcgc cattgacgat cgcggttctg aagggactcc tgacccccat tcttgaagac
    10441 ccggtcaact atgtgaatgc gccggtcgtc gccaaggaac gtggcatcga agtcaaagag
    10501 gtgaagatca gcgatgccgg agactttgcc agtgtgatcc gtgtgcgggt tgaggccggg
    10561 aagaaatctc atcaagtcgc cgggaccctc taccatcgca aggacccgcg gatcattgag
    10621 atcgatcagt tcaaggtgga agtggtgccg gacaaccatc tcctgctgat ccagaacgag
    10681 gatcgtccgg gggtgatcgg cacggtgggg catatccttg gcgatcacaa catcaatatt
    10741 gcgcgcatgc agtgttcacg ggaagagcgt ggcggcaaag ccctgcaaat tttcgggctc
    10801 gatgcgccgc ttcctaagtc agtcctcgat cagattacga acagcaagca catcctttcc
    10861 gtgaaggtcg ccgacctgtc gaaagggttg tagattgccc ggcagtcgac catgggcgct
    10921 ctttcgttga aggcttcctc ttcctcctcg cctctggtgg caggtcggga acggtcgctc
    10981 attcccgttg gaatgagcac cattcttcct gacgccgctc gacgtatccg acagctcgag
    11041 cagacgctgc tggccgtgct cgctcgcgcg ggttacgaag aaatcatcct gccgatgttc
    11101 gagtatctgg acgtcctcgc gccaggcctg gagtccgaac ttatcgagaa atgttatcag
    11161 ctggtcgatc ggacgacggg ccggttgatg ctgctgcgtc cggatgccac ggcacagatt
    11221 gccagaacgg tcgccatggg tatgatgggc gagcggcttc cgctgcggtt gtgttatcgc
    11281 acctctgtgt tccggtatga gcgtgagcat gcgggccgcg accgcgagat ttttcaagtc
    11341 ggagcggaac tcatcggcgt ggacggggtt acgggagatg ccgaggtgtt gacgctccta
    11401 ctggaatgtt tggcacaggt cgggttgtct tcattcaagg ttgctgtcgg ccacgtcgga
    11461 ttttttacgg ccctgctggt caaggccggc ttatcgcagg acggccagaa gcgtgtggag
    11521 caggctgccg cgcggaaaga catgccgctg cttgaggaac tgctgacgcg ggagggcgtg
    11581 tcccgatcga cggggcgggt cattcttgaa gcgctggaac tgtgcggtgg gcctgaggtc
    11641 ttggcgcacg ggagaaagtt ggtggggcgc gaccggaccc tattgagacc actcgatcgc
    11701 cttgcgcagg tctatgagcg attggtgtcg cccgggcagc aggcggtgtt gctcgatttg
    11761 ggcgagttcc gcgggtttga atattatgac gggatagtct tcgacgtctt tgcgccgggt
    11821 atcggggcgg agctgggagg cgggggccga tacgaccatt tgatgggccg gttcggtcgg
    11881 accgcggctt ctaccgggtt tgggctcgat gtcgatcgtc tgtttcgagc cctcgatgcg
    11941 tccggtgtgg tgacgccgcc tcactccagt ccctctacaa ccgctggctc caagccggtt
    12001 gcggcagaac cgcgacggcg caggagccgg gtatgaagtc gctctccgcg gttgatctca
    12061 cctcccccaa attgccgcca cagaacgtcg aggccgaaca gtcggtgctc ggcgcgatct
    12121 tgttggataa cacggcgatg gccaaggtga tggagctgct gacggacgag gagttttacc
    12181 ggactgcgca tcgcaagatt tatcaagcca tgctggagct gtccgatcgc ggcgaagtca
    12241 tcgatcagat tacgctcacg gaatgcttga aggcgcgctc tgagctcgaa gcggtcggag
    12301 gatccgccta tctagccgag ctcgttcagg tggtgccgac ggcggccaat gtccgctatc
    12361 acagcaagat cgttcgggac aaagcgctgt tgcgagggct cattcagacc tcgacagagg
    12421 tcgtgacgcg cgggtatgac gggacggtgg cagtcgatga gctgttggat tttgccgagc
    12481 gttcggtctt cagccttgct cagggaaagc tggatcgttc ctttactccg gtcaatcaga
    12541 tcatcaaaga gagtctcgat gtcgtcgaca agctgtccaa gcgcaaggaa cgtgtcacgg
    12601 gcgtaccgac cggctttatc gatctggatg acctgaccgc cggattacag ccgtcggact
    12661 tgatcgtcat tgccggccgt ccgagtatgg gcaagaccag tctggcgctt gggatggcgc
    12721 agcatgccgc gctgcatgcc ggcaccgtcg tcggcatttt cagcctcgaa atgtcgaagc
    12781 cgcaactcgt gctccgcatg ttgagttccg aggcccgcgt cgattcgcat gcgctccgga
    12841 ccggtcggtt gcaaaaggaa gactggtggc gcctcgcgga agcggccggg aagctggagc
    12901 aggcgccgat ctatatcgac gactccggcg ccgtgacggt gcagcagatg cgcgggaaag
    12961 cgaggcggct taaggctgag cgtggcctcg atctgttgat tgtcgactat ctccagctca
    13021 tgcaagggaa gagcgactcc gagtcacggc agcaggaaat ttccgacatt tcccgttcct
    13081 taaaaaccct ggcgaaagag ctgaacgtgc cggtcatcgc gctttcacag ttgagtcgcg
    13141 cagtggaagc ccggaagccg ccggttccca tgttggccga cttgcgggaa agcggtgcga
    13201 tcgagcagga cgccgatgtc gtgatgttta tctatcgtga agaagtctac gaaccggcga
    13261 cggagagaaa aggcatcgcc gaaattctgg tcagtaagca tcgtaacgga ccgatcggga
    13321 aacgggactt attctttcac gaccggtttg ccaagttcga gaatctcgag agtcgcgagg
    13381 tcgtttgacg cgttctttct cgggccggta taattcccat cagtcactgt ccttcgaata
    13441 ttccccctgc aagttgagcg gatgattgtg caacgattgt gtgaagagtg cccgcagcca
    13501 cccgcccgaa ggggttctgc gaatgtcaga acggtagctc ttctactcgc ggctatggcc
    13561 tgtgtcaccg ccgcctgcca gagcacgccg cctgcccgtc ccgctcctgc tgcggtccca
    13621 tccgccgtgc cggtggctcc tcgtgccatg ccctcatcct cagattcccg cgcgtcctat
    13681 cactttctcc tcgggtacca ggccgagttg gaccaggaaa cggaactggc gatcaaggaa
    13741 tatcaactcg cgctgcaaac agatcccgcc tccaattatc tcaaagccag gttggccgtg
    13801 ctctacttca ccgcggggga tgtgccgtcg gcggtgcgct ttgcggatga agtggccgac
    13861 gtgccggggc tggatgcgca gatgctgggg caaatcggcg gcatgtatgc ggcagcagga
    13921 aagcccgaca aggcgttgag actgttcaat cgtgccattg agcaggaacc tcagcgtagc
    13981 gagcatttct ttgccaaggg gctgttgcaa gccaatcaga aacaatatgt tgaagccgaa
    14041 gacacgattc gcgctggaat caagatcagc ccggactcgg cagtcgggta ctactattta
    14101 ggtcggatcg gggtcgaagc cagagatttt gacaaggcga cgactcactt tgagcaggcg
    14161 gtggcactga atccagcctt cgagcccgct tatgtggccc tagggtctgt ctatgaagcc
    14221 aagcaggatc gggacaaggc gatcgggatc tatcgtcgct acctgcaggg tgtaaatccg
    14281 aaaaatcgtg acatccggca tcatcttatt cgtctgcagg tgtcggcgaa gcagtacgac
    14341 gaagccctgc gagaactgca ggaaatgtta tctgaagatc cgtcagacct cgatgctcag
    14401 cttcggatgg ggcttgtcta tggggagcag aaagactacc ccaaagccat tcagcagttg
    14461 aaacaaatcc tcacggtgcg cccgacggaa ctgaaagtcc gtgactacct ggggtacttg
    14521 tatgaagaga ccaaggatta cgccaatgcg atggaggcct atcgttacaa tctgacgctg
    14581 gagccttcgt atttcgaggg gcatctgcat ttgggtgtcc tgcagtaccg gaccaagcag
    14641 tatgtcgaat ccattcagca ccttcgcgaa gccagccggt tgaatcccaa gcagccggag
    14701 gcccatatcg tcttggggtt gtcgcacttt caagtcgaac agtatgagcc ttccctccag
    14761 gcgtttcagg aagggattcg gcacaatccg gacaatgcgg acctgcattt taatgcggga
    14821 accgcgtatg acaagctgaa tcgattcgac gatgtggtga agtcgatgca gacgacgctc
    14881 gccttggatc cgcaccacgc cgatgctatg aactacctag ggtatagcta tgcagaacgc
    14941 ggcgtgaaga tcgaagaggc gattgcgctc accaagcagg ccgtcgcact tcgaccgacc
    15001 aacgggtatt acgtcgatag tctggcctgg gccttcttca agaagggcct gctggctgaa
    15061 gcgctggcgg agatgaagcg ggcggtcgca ctggttggcg acgatcccgt gatttatgaa
    15121 catctcggcg aaatctactt aaagcagcaa catctctcgg atggtcggga agcgctcttg
    15181 cattcgctgg aactggatcc ttccaatgac aagctgatgc agcgattccg tgaactgggt
    15241 ttgggcgatc ccgccaaaga agaacgcatt cgccaggcgc ttcggcgcgt ctcagatcgc
    15301 aagggtgcca ctccggctcc cgctcagcag taatccctcc atttttttcg ctccatcggg
    15361 catccgcgaa ccgggctgca tcgcctgcgg cctttccaca ccccgaacag atcgtctccg
    15421 tcgggtttca ctttaccagc ccagaatcgt tgggaccggc tgtccccgtt ccctaagagt
    15481 gggagccgtg actgttcgat tccgtcaatt tttggcactt tctcctatga attcaggtcc
    15541 gcgggggact gtcggacggc aatcgctgtg aaatcggaga gaaaagatca atgttgacgt
    15601 gatcttatca atcttcccgg cactcggcgg tttcgtggca taaggcctgc ttatagagtc
    15661 catgtctgca gggcgcctgt ggcgagggag tatgtaaatg ggtttccaag atgtctgtgc
    15721 ccaaatccca tggtgactac atcgaagcaa caagggttca cgctgatcga attgatggtc
    15781 gtggtggtga ttgtgggaat tctcgctgcc tttgccatac ccaactttct tcgataccgg
    15841 gcgcaggcga tgcaggctga ggcacgatcg aatttggcgg gtatcttcgt ggccgagacc
    15901 tcgttcttca gtgagcggaa agaatatggg aattttacag atgtgggctt tgccatcgcc
    15961 gaaggcggga cgaaccgcta cacctatcga tccggcctgg gaattggatc tgggatggga
    16021 ccgaacgggg gcaatctctg cgggtcatcc ggtagctgtg acacgattca gacggcgtct
    16081 ccgatggcgg gggcaatgac ttatacggga gcggttggta gcgcgacgac atccgcctct
    16141 ggatttacgg ccacagccgc ggcggatctc gatggagatg ccacgcatga cggctggtat
    16201 gtgaacgatg cgaagcaggg attgaacggg gcggagccca acgatgtgtc gagttagtga
    16261 gagcgcaaaa cttgtcaaaa gatgacggaa aatggaatgg ggttcaagcg gcgggaccag
    16321 aagttgaggg cataatggat ctaaataact ggaaaataga agaaatgata aggaactaca
    16381 ggaaggaggt gttgggcata gaagctgcta ttcccaagat ctgctaggct tgaaaaagct
    16441 gtgtcgtacg aagaggcaag agaagaacgt gtcggatgaa gtgacaacat taggaaaggg
    16501 aggaagtatg ttgaagcaaa tgaagggtca aaaaggtttt acgctgattg agttgatgat
    16561 cgttgtggcg atcatcggaa tcttggcggc catcgccatt cccaacttct tgcgctacca
    16621 ggcgaaatcc agacagtcgg aagccaagac caacctgggc gcgatcttcg tggcggagac
    16681 ggcctatctc agcgaacaag gacgctatgg aagcttctcg gaaattggtt acgcgttggc
    16741 gggggcgacg aatcggtata cctatcgcag ccctgccgcc cttggcggtg gtgcgtcgtc
    16801 agacactgct ctcatcgact acattgcctg cggcgctcct gctgcttgtg taaaccccgc
    16861 ggatggagcc acgggatctt tcgccagcct cgccgtcgtg agccctccga cctttacggc
    16921 ggcggcagtt gcaaatctcg acagtgatgg gaccatcgac ggctggtcgg tcaacgacat
    16981 caagggcggg ctgacgcagg cggttcctga cgacgtgacc agttaacgat cagtcgcagg
    17041 ctgtcttact gacgggggag gaaccaggtt cctcccccgt ttttatttcg gctgacccat
    17101 cgacgaattc ttcgatcaac tccctgacat gctccctgcc ggtttacggt ctgccatgcc
    17161 gcagtattta aattcgcttt gccaaattgt ccttgtctga taaagcagga gatgtctgct
    17221 gcgtcaagtg accaaagctt ctctatgaaa attctgcctt ccatgtcgct gctgatgatc
    17281 agcgccctcg tgatcttctc gcctctgcag gagggcggta cgactcatcc ggcgcaaatg
    17341 atcatcaggc tgttgattct agcgtggctg ggaatcgttc tggcgaccgg cattcgagcg
    17401 ggacggcttg ccgtccctgt gcttgcgatg aggtacgtcg tgctggcgtt tgtcggattg
    17461 gcactggtgg cgacgctttt ctcgccctac gcccattcga gccgacagtg gctgatcatg
    17521 atcatggggt acgtcacgat tttctatttg ctggtctctt gcgttgaccg gtgggagcat
    17581 gttcgtacgc tgaccgtgat gattgcgctg atgggtatag gcgaagcagg ctgggccatc
    17641 gtgcaaggat tagcgtggaa tgtcctgagg ccaggcggta cgttcttcaa ccccaacttt
    17701 ctcgccggat atctcaccgt gacctggacg attctgttga gcgtcgcaat ctatggctat
    17761 cgaccggtct cgacaggttc acgaccgtgg atgtctcccg tcctgtggtg gcttgggctg
    17821 ggtacggcgt taggcggtct gttccttgcg attctgttga cccagtctcg cggaggcatg
    17881 atcgtctgtc ttgtcgggac ggtttttgtg ctgacggccc ggtatggatg gaaattagcc
    17941 ggaagctgtg tggcggcagt ggtgttgctc gggctggttg ttccgacgcc gattcgcgat
    18001 cgagtgttgg cggagcatca acaaaatcct gtgtcctacg ctcgctggca gatgtggcag
    18061 ggagcggtcg cacagatggt cgatcatccg ctgggaatcg ggctaggcct ctatcagtat
    18121 acgtatcccc aatatgcgtt tccggtcgat ggtgaaattg cccggtttgg aaaagtcgcc
    18181 cagacaccgc acaacgatta ccttcagttg ggcgtcgaga tggggcctgg agcgatcctt
    18241 gtgttcggga gcggggttgt cctgctcttt cgcgaactga ggcacgtgct tcacgcgcga
    18301 ctacggcggt ggcagaggag cctcatcgtg ggtgtgggag gtgggggtat cgcgttactg
    18361 gtccatgccg cgcttgattc gagtttgcgt gagtcagccc ttgcgatcct gctggtgctc
    18421 tgtggcgcag tgatcgtgtc ggcggctcgg ctcacccgaa gggggaccga tgccgtctat
    18481 gtcgtaccga ttcattcacg ctgggcctgg ggaatcgggg cggcctgtct ggtgctggtt
    18541 gccggcgcag aggtgacgcg aatgggtgtg gcctggataa agtttgaaag ggcttcgcgt
    18601 caggccatgg caggagagac cgttgcggcg atcgagggat tgaaggcggc tgtagcactg
    18661 gatcctggca aggcgttgta ccaccatggt ctcgggtcag tctacgcgag ggcctttgaa
    18721 tcttcccgcg agaaacaggt tttccaactg gcgtatgcgg agtttaaaca agcaatcgaa
    18781 ctgaatccgc tcgatagccg gctactcggc ctgctggggc aactctatgt ttcggcggcg
    18841 cagacgtcct cttctccggc ggcattcgat gcccaacaaa aagcctggtt acgtgctgcg
    18901 gttcaagtct acgaacgggc catccagctc gcaccgtttt ccgcgatgta tcgatacgaa
    18961 caagcgcagc tccactggat gcttggagag cggagcgaag ctgaacaacg agcgaaagag
    19021 gcggagaatc tggagccgaa ttttctgcct gcccgtgcat tgctggctcg cttgtgggta
    19081 gatgaggggc gggtcgacca ggcaaggggt cagcttcgtg aaattctggc gcggcaggcg
    19141 cgctacaagg agtggagcaa gaatggtctc gaccaggcgt ttctgaatgt cgacgtcgcc
    19201 ccgcttcgtg ccgcagtcgg agaaaaggcg gtcgctggat gaagggcaca agcatgaaac
    19261 agggactgat tcatgcggcc ttgggagtga ttctgctcgc aggctcctcg gtgctgctcc
    19321 atcttctgga tggccaacga actgcggtcg cgcgagccga gcagttggcc tatctcccga
    19381 aaggggacta tctgaaattg gccgtgctcg ggtatcggca agtcgtcgca gacctgatct
    19441 ggctgcaggc ggttcaacat atcggcgcga agcgggatac gcaggcgggg tatatgtgga
    19501 catatcacgc cgtcgatgtc ctgacggatt tggatccgac gtttgttccg ccctaccaag
    19561 ccaccggact ctttctgggt gtcttggtcg gtcggcatga agaaggtgtg gcgatcctga
    19621 agaagggtat tcagcacaat ccgtcacatt ggcagctgcc gttcctggcc ggctatatct
    19681 cgtactacga gcgctgtgat ccggcggcgg gtggggaatt ttttcggatc gcggcgcgag
    19741 tgcccggtgc gcctgcgtac cttccgcaat tggccgcacg tatgacggtg gagagcgggg
    19801 actcctccgc ggcgctggaa ttcctggagc gtttttcccg gagcgtcacg gatgagcggg
    19861 tgcgagaggc attgtttcgg cggatgaaag aaatcgtgca agaacaggat ctacgcgttc
    19921 tcgaagaggg gatcagtcgg taccgagcgc actatgggca ggcgccggca aaactggaag
    19981 atcttctgtt gcgcggtatc atcaccagcc tgccgatgga tccgttgggc gggcagtatg
    20041 agctcaatag tttgaccgga accgtcagcg cctcctccac gcgcgagcga cttcggattc
    20101 atgagaaagt cgcgtgccga ttgggacgtg cgacgcagtc ggagggaggg gccggttcga
    20161 tggagactcc gttaccgact gtgcagtgag aatggaggcg cgctgtggat gatatcgtca
    20221 ccgacaactt gaccaaatcc tacgcctccg gttggcccgg gcgaccgccg ttcgtcgcgc
    20281 tggacggatt gtcactcacc gttcgccgag gagagatttt cggcttcctg ggaccaaatg
    20341 gagcgggaaa aaccaccacg ctgaagattt tgctggggct tgttcgggca acgagcggtc
    20401 aggcgtactt gctggggcag ccggccggtg atgtggcggc gaggcgccga attggctttt
    20461 tgccggagtc tccctatttt tacgactatc tcaccgccga agagtttttg gggttttatg
    20521 gtcaattggc aggtttggcc cgcagtgcca tcacccagcg ggtcacggac ctcttggggc
    20581 tcgtcggcct cacagacgcc cgtacccggc agttgcgcaa attctccaaa gggatgttgc
    20641 agcgtgtcgg cttagcgcag gcgctgattc acgaccctga attgatcatc ctcgacgagc
    20701 cgatgactgg cctggacccg gtcggacgca agcaggtccg cgatctgatc ttgaacctgc
    20761 gcgatcgcgg gaagaccgtc tttttcagta cacacattct
//
LOCUS       DAORQN010000018        19876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_185_length_19876_cov_9.064124, whole genome shotgun sequence.
ACCESSION   DAORQN010000018 DAORQN010000000
VERSION     DAORQN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185_length_19876_cov_9.064124"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1599
                     /locus_tag="PK614_02530"
     CDS             55..1599
                     /locus_tag="PK614_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRB81117.1"
                     /translation="MNDFQIPTEQQPPRRSWIFPALLLIAGVLIGVILTSDLGWLPTG
                     HAVPEPVPPPAATPVSTAVQPTLPGAGGQSFVDVAKVVKPAVVNIFATRNGPSEEGKG
                     TPFEDPFFRRFFGEEWMKRFEAPKERKERGLGSGVIVDPNGLIITNNHVVNKADEIKV
                     FLSDKREFKAKLVGTDAKTDVAVLKIEAGGLPTVAWADSDKLEVGEFVLAVGNPFGLT
                     QTVTLGIVSALGRAAGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIYSQSG
                     GNMGIGFAVPSNMAHSIMEQLVQHGKVVRGWLGVSIQELTPELSSQFGVPKDVKGVLV
                     SDIMDDSPAKKAGFERGDVIVEYDGKPMDSPAHLRNAVAQTVVGKKVTVKLIREKKPK
                     SIELTIAEQPKNLAQAGVEEGGESMAPAGLLSDIEVRELNTELAGRYGLKSSDRGVVV
                     VRVKPGSPAEEAGVREGDLVLEVNRKAVGTLKAYEHVAANLPKDQAVLLLLRRQGRAI
                     YLTLRP"
     gene            1650..2675
                     /locus_tag="PK614_02535"
     CDS             1650..2675
                     /locus_tag="PK614_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRB81118.1"
                     /translation="MVTRAIFVLLSALAGMALFLRAQDPSREFLLMGLGMGAVAGGLI
                     LAGEYALRRLSFGIIVGGAVGLAGGLILTGLVEWVGSAVFDVETFLFHIGGLVFLLGL
                     PYLGLAMGARFGNEQFPGLDQGPTVAGASHASLKVLDTSVIIDGRVADLCETGFLEGP
                     FLVPHFILNELQHIADSSDSLKRARGRRGLDILNKIQKMPGIDVRIIDEDFPHVKEVD
                     AKLVVLAKKVGGRIVTNDLNLNKVAELQGVRVLNINELCNALRPVVLPGETIRVFVLK
                     EGKEAGQGVAYLDDGTMIVVDNARRCIGRNVDVTVTSVLQTTAGRMIFTRLKEESERE
                     EYQVARG"
     gene            2795..3538
                     /gene="ispD"
                     /locus_tag="PK614_02540"
     CDS             2795..3538
                     /gene="ispD"
                     /locus_tag="PK614_02540"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRB81119.1"
                     /translation="MSVRNAKPSLAGPRTVALVPAAGRGLRMGGHIPKQFLALGGRPI
                     LAQSLLVLQASPLIHEIILAVPQSERQYCLDHIVATGEFSKLTKVVPGGAQRQDSVRH
                     ALAEVSQETEIVLVHDAVRPFLTEDMIRRVVAAAVEHGAAIIALPMRDTVKYVGAGGV
                     IERTVDRRPLWLAQTPQAFRRDWLEEGHHKAFLAGVQATDDAHLVELIGKPVVVVEGS
                     GENIKVTRPEDLVIGEAILGSRAAARSAE"
     gene            3535..4017
                     /gene="ispF"
                     /locus_tag="PK614_02545"
     CDS             3535..4017
                     /gene="ispF"
                     /locus_tag="PK614_02545"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRB81120.1"
                     /translation="MTAVRVGCGWDIHPMVAGRKLILGGLEIPHHKGLQGHSDSDALV
                     HAICDALLGAMGEGDLGRHYPSSDQRFKNISSLKLLEDVVGKLRAKGYRLANIDSTII
                     AQAPRLSPHLAAMQKIIAGVLQVDPDLVNVKVKSGEGLDAVGREEAIAAQAVCLIERG
                     "
     gene            4057..4707
                     /gene="cysE"
                     /locus_tag="PK614_02550"
     CDS             4057..4707
                     /gene="cysE"
                     /locus_tag="PK614_02550"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRB81121.1"
                     /translation="MLKTISQDLQAIFDRDPAATSRLEVILTYAGFHALLAYRLAHWL
                     KARRVPFVPRAISQLARWLTGIEIHPSAKIGERFFIDHGMGVVIGETAEVGDNVTLFQ
                     GVTLGGTGKERGKRHPTLGSHVVVGAGAKILGGITIGDNVKIGANSVVLKSVPPNSTV
                     IGVPARIIKSQGERLPDATMDHTNMPDPTADRFAALELELIELRKKLENRDGNGPR"
     gene            complement(4852..5169)
                     /locus_tag="PK614_02555"
     CDS             complement(4852..5169)
                     /locus_tag="PK614_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HRB81122.1"
                     /translation="MNHLCTTLSVLMLAAVLATPQLSYAGAQQNKMKTCNADANAKGL
                     TGEGKGEERKAFMKECLSAKPEKAAAGTSQQNKMTTCNKDAKAKKLAGDERKKFMKEC
                     LSN"
     gene            complement(5251..6012)
                     /gene="truA"
                     /locus_tag="PK614_02560"
     CDS             complement(5251..6012)
                     /gene="truA"
                     /locus_tag="PK614_02560"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRB81123.1"
                     /translation="MTTFKVVLEYDGTQYAGWQRQLNVPTIQAAVEEALAAIAQTRLT
                     IIGAGRTDAGVHAFGQVASFRTDRGLSQREWLRALNAHLPADISALSVEVVPDHFHAR
                     YSAKGKLYEYHLMNRSERAPLLRERAWMLYKPLDFAAMTEAAACLTGSHDFSSFETAP
                     TDNENPQCRLQQADLRRRGDLIILSFYADRFLKQMVRAMVGTLVEVGQGKRTAADMRT
                     VLAARARAAAGRTAPAHGLYLVRVDYTDGNIPSLP"
     gene            complement(6060..7388)
                     /locus_tag="PK614_02565"
     CDS             complement(6060..7388)
                     /locus_tag="PK614_02565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRB81124.1"
                     /translation="MSHVASLIRHLLTLAFACVLFGAGISSGGEIQTEPSWPTVPQVA
                     AEEPSPRFLLVKQERAGASRYPIIVRDLRTWSTTDGTRLVLDLNHKTSFSETHLRNPD
                     RVLIEVNNAILGKSSRQRVSGGTIPQSFQIAQSRPRVVSITLTRNQNSRYKVFSLDNP
                     DRLVIDITQRPKDQIRQIGETSATSPQAPAVPLPTPPAIPSPTVTEPARPVAPKPASP
                     VQTIVIDPGHGGKDPGTIGQHGTTEKEVTLKVGLLLKSLLSTVPNTRVLMTRERDAFI
                     ELEDRAKFANGKDADLFISIHVNSHPHKGVRGLEIYHFGEAKDQRALEVAARENGTPL
                     NNTGVGWEYIVADLLTSKKIEHSQELAWTAKQAMVTNLNGRYSTIDHGVKTAPFYVLR
                     FTTMPSILAEIAFMSNPAEEEQMRSQPFLAHIAESIFEGVKTYLHTNPPR"
     gene            7482..8015
                     /locus_tag="PK614_02570"
     CDS             7482..8015
                     /locus_tag="PK614_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRB81125.1"
                     /translation="MATHLLVDGYNLVGSAGMALAAGSGRLEAVRETLLKNLAGYRQR
                     KGHAITVVFDGWQGGLGAEHREFQSGVEVVYSKRGERADQVIQRLARLYGRDCAVVSS
                     DHEVVNSARVAGAFVIGAAEFRAKLQDRSSSLSTVAFKELDRGDLDRVKRSKDKGGNP
                     RKLPKSQRKRNHQLREF"
     gene            complement(8002..8781)
                     /locus_tag="PK614_02575"
     CDS             complement(8002..8781)
                     /locus_tag="PK614_02575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRB81126.1"
                     /translation="MLIDTHTHLDDARYESDREAMIARARAAGVESMITIGCDLATSR
                     SAVTLANQYPFVYASIGVHPHEVKHIEDGWYEEFRRLARDKKVVAYGEIGLDYHYNHS
                     DPELQRTRFREQIQLARELKLPVIIHTREAQDDTIRILKEERASEIGGVFHCFSGDAW
                     LAKDAIELGFYLSFSGILTFQNATMLREIAKTVPADRLLIETDCPYLTPVPHRGKRNE
                     PAFVRHVAELLATITADNAPSTAEDVARRTSENARRLFKIP"
     gene            complement(9083..17782)
                     /locus_tag="PK614_02580"
     CDS             complement(9083..17782)
                     /locus_tag="PK614_02580"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRB81127.1"
                     /translation="MSRTDILASWRQLTGRLSGQPATMPYAGDEPPLRSELFSAEQMQ
                     QHGKTLAGLHAVSAEHEPDPLLARLAENEVVLLDVRNLVAEAVKGNRRITPAGEWLLD
                     NFYLIEEQIRLAKRHLPKGYSRELPRLGNGPSAGLPRVYDIALEIISHGDGRVDPESL
                     SGFVAAYQSVTTLKLGELWAIPIMIRLALIENLRRAGARIASDRVDRNRAHEWAGQMM
                     ETAEKDPKSLILVIADMARSNPPMVSAFVAELARRLQGQSAALALPLTWIEQRLSESG
                     LTIEQLVQSETQQQAIDQVSMSNSIGSLRFLAAMDWREFVEAQSLVEQTFHEDPAGVY
                     GQMDFATRDRYRHAVEKLAKSSPLSEGDVARQAILLAQTGAAAEGPDHRTAHVGFYLI
                     DNGQTQLEQAVSLRRSAFDRLRRWTGSSSVPIYLGSILFLTATFTGCFLALAIAAGWN
                     GWPLALLGFVSALAASQLAVTLVNWIATALVTPHPLPRMDFSDGLPPALRTLVVIPTL
                     LQDVRGIDDLLEALEVRFLANRGSHLHFALLTDFQDADQETVAGDDQLVLAAGVGIDA
                     LNAKYAGEQGDTFFLFHRPRRWNAHERLWMGYERKRGKLADLNGLLRGGSTDRFSAIV
                     GQPDILSTVKYVITLDTDTQLPRDSARQFVAAMAHPLNRPQYDPAVQRVTAGYGILQP
                     RVAVSLPGTNRSRYARLNAGEPGIDPYTRAVSDVYQDLFGEGSFIGKGIYEVDAFEQA
                     LSDRFPENRILSHDLLEGSYARAGLLSDVQLYEEYPARYNRDVTRRYRWIRGDWQIAR
                     WAFPRVPGPNGHTRSNPLSTLSRWKIFDNLRRSLVPAALTSLFVSGWTMLSPVWLWTL
                     ATLGLFLIAPLLGAVVDLCRKPEDMRMSQHLTATARGMTQQLTQALLTLMCLPYESFY
                     SLDAIIRTAGRVWFNRTGLLEWNPSGATDRSRTDLIGSYRSMWIGPAMALIITIILMQ
                     TRAEALLIAAPVLSLWALSPLFTWWISKPLARREARLTADQTMFLRKMARKTWAFFET
                     YVSPEDHWLPPDNYQEHPAPKVAHRTSPTNIGLALLANLSAYDFGYLSAGQLIERTAH
                     TFDSMATLERFRGHFYNWYDTQTLKPLLPMYISSVDSGNLAGHVMTLHSGLLSLPEDK
                     ILAERTFEGLRDTLALLSEALETPTSQVDALQKNLLAASDNRPTTLSEAHHTFTLLTT
                     QVDEVTAHLDPASNAEAHRWAHAFARQCRNAVAELTILAPWIGLAAADEILQVYPGLD
                     QIPTLRTLTRLEGEWLPAIDARLGPDVSSTERTWLIELRRHLSAASRLAEQRLASLDH
                     LARQANQFAQMEYDFLFDDTRFLLSIGYNVAERRRDASYYDLLASEARLCSFVAIAQG
                     QLPQESWFALGRLLTTTGGEPILLSWSGSMFEYLMPLLVMPTYQQTLLDQTYRAAVKR
                     QIEYGRERDIPWGVSESGYNMVDAQLNYQYRAFGVPGLGLKRGLGEELVIAPYATSLA
                     LMVAPEEACLNLQRLTAEGADGPYGLYEAIDYTPSRLPRGQSRVIIRSYMAHHVGMSF
                     LSLAYLLLGRPMQKRFEADPLFQASTLVLQERIPKATAFYAHSTELSDLRTTSGTADT
                     PVRVLTTANTPVPDVLLLSNGRYHVMVTNAGGGSSRWKDLAVTRWREDSTCDNWGTFC
                     YLRDVESGEFWSTAYQPTLKPSKTYEVIFSEGRAEFRRRDHEIETHAEIVVSPEDDIE
                     LRRIRLTNCSDTKRMIDLTSYAEVVLALPAADALHPAFSNLFVQTEIIRERQAILSTR
                     RGRSQDEHAPWAFHLMAVRGPHSGEMSYETDRLQFIGRGRTLAAPQAMDHSGPLSGNQ
                     GSVLDPIVSIRCPIVLEPEESVTVDLVSGIGENRDLCLRLVDKYQDRRLADRVFELAW
                     THSQVVLRQLNTTEADAQLYGRLAGSILYANASLRAEAAVLSKNRRGQSGLWGYAISG
                     DLPIVLLQIGDPSNIDLVRQMVQAHAYWRLKGLAVDLVIWNEDHAGYRQLLHDQIMGL
                     ISAGVEANVMDRPGGIFVRTAEQMSVEDRLLQQSVARVILTDRRGTLADQITRLASPA
                     APAYRFKPTRTHRGDPPATIEPRTDLLFYNGLGGFSSDGREYVITTTAEQVTPAPWVN
                     VLANPHFGTVVSENGLAYTWSENAHEFRLTPWHNDPVTDSSGEAFYLRDEESGHVWSP
                     MPMPCRGTSPYVSRHGFGYSCFEHTEGGIRSEVWVYVALDASVKFTVVKLRNESGRPR
                     RLSATGYVEWVLGDLRPKSVMHVISEIDPATGALFARNPYNTDFPGRIAFFDVDEGTR
                     SMTGDRTEFLGRNGTLRNPASMSRSRLSGKVGAALDPCGAIQMPFDLAVGQERDCTFR
                     LGVGKDTEDARQLVRRFRGATARRAALETVWHHWTHTLGAVHVETPDQSLNVLANGWL
                     LYQTIACRLWARSGYYQSGGAFGFRDQLQDVMALVHAKPHLAREQLLLCAGRQFKEGD
                     VQHWWHPPSNRGVRTRCSDDFLWLPYVTSRYVMTTGDTGVLDTPIQFIEGRPINADED
                     SYYDLPGRSEQSGSLYDHCVRAILKGLTKGVHGLPLIGSGDWNDGMNMVGDRGQGESI
                     WLGFFLYDVLMRFAEVAKTTGDVPFAERCQAEAAQLQQQIEQQGWDGEWYRRAYFDDG
                     TPLGSATNDECQIDSIPQSWSVLSAAGEATRSRLGMEAVYRRLVRRDDALIQLLDPPF
                     DKSALNPGYIKGYVPGVRENGGQYTHGALWATMAFAALGDRERAWELFRMINPVNHAQ
                     SPGDINRYKVEPYVVAADVYAVSPHIGRGGWTWYTGSAGWMYRLIVESLLGLKLEGNR
                     LRFIPCLPRDWKAFKVHYRFRETLYHITVRQSGTDQQAVSVMVDGIQQADHAIRLVDD
                     RREHSAEVTIPSA"
     gene            complement(18605..19663)
                     /locus_tag="PK614_02585"
     CDS             complement(18605..19663)
                     /locus_tag="PK614_02585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRB81128.1"
                     /translation="MNRPIDNQHVIEIKPLPSPRDVKTQLPISDEVSELVFQTRQAIR
                     NILHGRDMDRLIVIVGPCSIHDPDAAYEYADRLKPVADAVRDKLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIAQGIQLARTILLNINGKGLPCATELLDPVTPQYIADLLSW
                     TAIGARTTESQIHREMASGLSMPVGFKNGTEGSLQVAINAMITSRSPHHFVGVNADGI
                     TSIIKTTGNPDHHIVLRGGGGRTNYSVEDIARAEAAVASEGLARGVMVDCSHDNSGKN
                     HQRQVEVAGEVLKQFQQGRRAIMGLMLESHLQGGRQTWDPNKALTYGMSITDSCLAWS
                     DTEALLYGMAESLATRPV"
BASE COUNT         3814 a         5605 c         6410 g         4047 t
ORIGIN      
        1 ggactgtatg cgggagagaa gccctaaccg gcgcctccgt ccgcacggat tacaatgaac
       61 gactttcaga ttccaacgga gcagcagcct ccgaggcggt cctggatatt tcccgcactg
      121 ctcttgatcg cgggggtatt gatcggggtc attctgacct ccgatctcgg ctggttgccg
      181 accggacatg cggttccgga accggttcct cctccggccg caaccccggt ctccacggcg
      241 gtgcagccca cgctgcccgg cgcgggtggg cagagtttcg tcgatgtcgc caaagtcgtg
      301 aagccggccg tcgtgaacat ttttgcgacg cggaatggac ccagcgagga ggggaagggc
      361 accccgtttg aggatccatt ttttagacga tttttcggcg aagaatggat gaagcggttc
      421 gaggcgccga aggaacgcaa ggaacgaggg ttggggtccg gcgtcatcgt cgatccgaac
      481 ggtctcatta tcaccaacaa tcatgtggtg aacaaggctg acgagatcaa ggtcttcctc
      541 tccgacaagc gtgagttcaa ggccaagctg gtcggcacgg atgccaagac cgacgttgcc
      601 gtgctgaaga tcgaggccgg tggattgccg accgtcgcct gggccgattc cgacaagctg
      661 gaggtcggtg agtttgtgct ggcagtgggc aatccgttcg ggttgacgca gacggtgacg
      721 ctggggatcg tcagcgccct tggtcgcgcg gccggcatcg ccgaatatga agacttcatt
      781 cagaccgatg cggcgatcaa cccggggaac tccggcggcg ctctggtgaa tgtgcgcggt
      841 gaactggtcg gcatcaatac agcgatctat agccagagcg gcgggaacat gggaatcggc
      901 tttgccgttc cgagcaacat ggcccattcc atcatggagc agctggtcca gcatggcaaa
      961 gttgtgcgcg gctggttggg tgtctcgatt caggagctga cgccagaatt atcttcgcag
     1021 ttcggcgtgc cgaaagacgt gaagggcgtc ctcgtcagcg acatcatgga tgacagtccg
     1081 gcgaagaaag caggttttga gcgcggcgat gtgatcgtcg aatacgacgg gaaaccgatg
     1141 gattctccgg ctcacctgcg gaatgccgtg gctcagacgg tagtggggaa gaaggtgacg
     1201 gtcaagctca tccgcgaaaa gaagcccaag tccatcgaac tcacgattgc tgagcagccg
     1261 aagaatctgg cgcaagccgg ggttgaagag ggcggagaat cgatggctcc cgccggctta
     1321 ctctcggaca tcgaggtgcg ggagttgaac acggaactgg ccggccggta tggcttgaag
     1381 tcgagcgatc gcggcgtggt ggtggtgcgg gttaagccgg ggagtcccgc ggaagaagct
     1441 ggtgtgcgtg aaggggatct cgtactggag gtgaatcgca aagccgtggg gacgttaaag
     1501 gcgtatgaac atgtcgccgc gaatttgccg aaagaccagg cggtattgct cctgttgaga
     1561 cggcaggggc gagccattta tctgacgctg aggccgtgat cgcaccgaat cgtccgttcg
     1621 gggttggtca cagagaggag gcaaacagta tggtaacacg ggccatattt gttcttctca
     1681 gcgctcttgc cggcatggcc ctgtttcttc gggcccagga tccaagtcgc gagtttttgt
     1741 tgatgggatt gggaatggga gcggtggccg gtgggctcat tctggccggc gagtatgcgc
     1801 tccgcaggct ttcattcggc attattgtcg gcggtgccgt ggggctcgcc ggtggtctga
     1861 ttctgacagg tttggtggag tgggtcggga gtgcggtctt cgatgtggag accttcctct
     1921 ttcatatcgg cgggttggtg tttctcttag ggttgcccta tctggggttg gccatgggcg
     1981 cgcggttcgg caacgaacag ttccccggtc tggaccaggg gccgacggta gccggggctt
     2041 cccatgccag ccttaaagta ttggatacga gcgtcatcat cgacggccgg gttgccgacc
     2101 tctgtgagac agggtttctg gaagggccgt tcctggttcc ccactttatc ttgaatgaat
     2161 tacagcacat tgcagactcg tcggattcct tgaaaagggc gcgcgggcgt cgcgggttgg
     2221 atattctcaa caagattcag aaaatgccgg gcatcgatgt ccggatcatc gacgaagatt
     2281 ttccccatgt gaaggaagtg gatgccaagc tcgtggttct agcgaagaaa gtcggggggc
     2341 gcatcgtcac gaatgatttg aacctgaaca aagttgccga attgcagggc gtgcgggtgt
     2401 tgaacatcaa tgagctctgt aatgcgctgc gtccggtcgt gttgccgggc gaaaccattc
     2461 gggtctttgt cttgaaggaa ggcaaagagg cgggccaggg cgtcgcatac ctggatgacg
     2521 gcacgatgat tgtcgtggac aatgcacgac gttgtatcgg ccgtaatgtg gatgtgaccg
     2581 tgaccagtgt gcttcagacg acggcggggc gcatgatctt cacccgtttg aaggaagagt
     2641 ccgagcgcga agagtatcag gttgcgcgtg ggtagtttgg cgagagggaa gagcggtggg
     2701 ccaatggaat cctccgtcct ccggggcggc gcatcgagct cacacgattg cctaggtttg
     2761 gccttgtccc cttgctgctc acgtgagtgt gccggtgtcc gttcgtaacg cgaagccgtc
     2821 gctggcaggg ccgcgcacgg tggccctggt tccggccgcc ggtcgtgggc ttcgcatggg
     2881 cggccatatc cccaagcagt ttcttgcgtt aggggggcgg ccgattctcg cgcagtccct
     2941 ccttgtgctc caagcttccc cgctcattca cgagatcatt ctggcggtgc cccagtcgga
     3001 acgtcagtat tgcctggacc atatcgtggc gacgggtgag ttcagcaagc tgaccaaggt
     3061 tgtgccgggg ggagcgcagc ggcaggattc cgtgcgtcat gccctggccg aggtgagtca
     3121 ggagaccgag attgtcctgg ttcatgacgc ggtgcggccg tttctcaccg aggacatgat
     3181 ccgcagagtc gtggctgcgg ctgtagagca tggggcggcc atcatcgcgc tgccgatgcg
     3241 cgacacggtg aagtatgtgg gggccggcgg ggtgatcgaa cggaccgtcg accgccggcc
     3301 tctctggttg gcgcagacgc cgcaggcgtt tcgccgtgac tggttggaag aggggcatca
     3361 caaggccttc ttggccggtg tgcaggcgac ggatgacgcg cacctcgtgg aactgatcgg
     3421 gaaaccggtc gtggtggtcg aagggagcgg agaaaatatc aaggtgacga ggccggaaga
     3481 tctcgtgatc ggagaagcga ttctcggctc acgagcggcg gcaaggagtg cggaatgacg
     3541 gctgtacgcg tgggatgtgg atgggatatt catccgatgg tggcgggacg aaaattaatt
     3601 ctgggtggac tcgaaattcc gcaccacaag gggctgcaag ggcattccga ttccgatgcg
     3661 ttagttcatg ccatttgcga tgcgttgctc ggggcgatgg gagaaggtga tctcgggcgt
     3721 cactatccga gctcggatca gcgattcaag aacatctcaa gtctcaagtt gctcgaagac
     3781 gtcgtcggaa agctgcgtgc aaaggggtac cggctagcca atatagacag cacgatcatt
     3841 gcgcaggcgc cccggttgag cccgcatctg gccgccatgc agaagattat tgccggggtg
     3901 ctccaagtcg atccggacct ggtgaacgtg aaagtaaaaa gcggcgaagg gttggatgcg
     3961 gtggggcgtg aagaagcgat tgcggcgcag gccgtctgcc tgatcgagcg aggctagagc
     4021 ctatccgctg gtgcgctcac gagtctggta ccacctatgc tcaagactat ctctcaggac
     4081 ctccaagcga tttttgaccg ggaccctgcc gccaccagca ggctcgaagt catcttgacc
     4141 tatgcgggat ttcacgcgtt gctggcctat aggcttgcgc attggttgaa ggccaggagg
     4201 gttccgtttg tcccgcgcgc gatttcccaa ttggcccgct ggctgaccgg aatcgagatc
     4261 catccctccg ccaagatcgg cgagcgattt tttatcgatc acggcatggg tgtggtgatc
     4321 ggagaaacgg ctgaggtcgg agacaatgtc actctgtttc agggcgtgac gcttggtgga
     4381 acggggaaag agcgcggcaa gcgccatccc acgctcggca gccatgtggt cgtcggggcg
     4441 ggggcgaaga tcctcggcgg gattacgatc ggcgacaacg tgaaaatcgg cgcgaattct
     4501 gtcgtcctga agtcggttcc ccccaattcg acggtgatcg gagtgccggc caggatcata
     4561 aagtcgcaag gggagcggct tcccgatgcg accatggatc ataccaatat gccggatccg
     4621 accgccgacc gatttgccgc gctcgaactg gaactgatcg agttgcgaaa gaaactcgag
     4681 aatcgggacg gcaacggccc gcgttagcat gtcctcactt ctgcgctaat caaccccttg
     4741 cttctcagta ctgagagact ttgcgactgc tggagcgcgg acagcgcaag tcaatcatcc
     4801 gctgcctgtc gagccccggc atagtcatat cgtccagccg gggcactgtg cctagttgga
     4861 caggcactcc ttcataaact tcttacgctc gtcgccggcc agtttcttgg ccttggcatc
     4921 cttgttacag gtcgtcatct tgttttgttg tgaggtgcct gcagcggctt tctccggttt
     4981 agcggagaga cattccttca tgaatgcctt gcgctcctca cccttgccct ccccggtgag
     5041 gcccttggca ttcgcatcgg cattgcaggt tttcattttg ttctgctgcg cgccggcgta
     5101 gctgagctgc ggcgtggcga gcacggctgc caacatgaga accgacaagg tcgtgcagag
     5161 atggttcatt actggctcct ttcgaggtga gcgtgggttg tgtgcgggac tataacacaa
     5221 ggcgcccgac ctgtcccgcg ggggagttcc ttaagggaga gaggggatgt tcccatcggt
     5281 atagtccact ctgacgagat agagcccgtg agcgggggcg gttctcccgg ccgcggccct
     5341 tgcccgcgca gcaagcacgg tgcgcatgtc ggcggcggta cgcttgccct gccccacttc
     5401 caccagggtt cccaccatgg cacggaccat ctgcttgaga aaccggtcgg catagaagga
     5461 gaggatgatg agatcccccc ggcggcgcag atccgcctgc tgcagccggc actggggatt
     5521 ctcgttgtcg gtcggcgccg tttcgaatga ggagaaatcg tgcgagccgg tcaggcaggc
     5581 ggcggcttcc gtcatggccg cgaagtcgag cggtttatag agcatccagg cgcgctcacg
     5641 gagaaggggc gctcgctcag accgattcat cagatgatac tcgtacagct tccctttcgc
     5701 cgagtagcgc gcatgaaaat gatccggcac cacttcaacc gagagggcgc tgatatcggc
     5761 aggaaggtga gcgttcaacg cgcgaagcca ctcgcgttgc gagagcccgc gatcggtcct
     5821 gaagctggcg acctgcccga aggcatgaac cccggcatcg gtccggcctg cgccgatgat
     5881 cgtgagcctg gtttgggcga ttgctgcgag ggcctcctcc accgccgctt ggatagtcgg
     5941 cacgttcagc tggcgctgcc acccggcgta ctgtgtgcca tcgtactcga ggacgacttt
     6001 aaatgtggtc ataacatgga atggaaagaa ggaactctag cccaacggga aggcgcctgt
     6061 catctggggg gattggtgtg gaggtaggtc ttgacccctt cgaaaatcga ttccgcaatg
     6121 tgcgccagga agggctgtga ccgcatctgt tcttcttcgg cgggattcga catgaaggcg
     6181 atctcggcca gaatgctggg catggtggtg aagcggagca cgtaaaatgg cgcggtcttc
     6241 accccgtgat cgatggtgct gtaacggccg ttgagattcg tgaccattgc ctgcttggcc
     6301 gtccaggcga gttcttggga gtgttcgatt ttcttggagg tcaggaggtc ggcgacgatg
     6361 tattcccagc ccaccccggt attgttcaac ggcgtgccgt tttctcgcgc ggccacttcc
     6421 agggcgcgct gatccttcgc ttcgccgaaa tggtagatct ccagcccgcg gacgcccttg
     6481 tgcgggtgcg aattgacgtg gatcgagatg aacagatccg cgtctttgcc gttcgcaaat
     6541 ttggcccggt cttccagctc gataaaggca tcgcgttcgc gggtcatcag gacgcgtgta
     6601 ttcggcacgg tactgagcaa ggatttcagc agcaggccga cctttaaggt cacctctttt
     6661 tccgtcgtcc cgtgctgtcc gattgtgccg gggtcttttc ctccatgccc ggggtcaatg
     6721 acgatcgtct ggaccgggct tgccggcttg ggtgcaaccg ggcgagccgg ttccgtgacg
     6781 gtggggcttg gaatcgccgg cggagtcggc aaggggacgg ccggggcctg tggcgaagtc
     6841 gccgaggtct cgccgatctg ccggatctga tcttttggac gctgcgtaat atcgatgacg
     6901 aggcggtccg gattgtcgag cgagaacacc ttgtaacggg aattctggtt acgcgttagc
     6961 gtgatgctca ccacgcgcgg gcggctttgc gcaatttgaa acgattgcgg aatcgtcccg
     7021 cccgagacgc gctgccgtga tgattttccg aggattgcgt tgttcacctc aatgagcacc
     7081 cggtcggggt tgcgcaaatg cgtttcggag aaggacgtct tgtgattcag gtcgagcacg
     7141 aggcgtgtgc catcggtggt ggaccaggtg cgaagatcgc ggacgatgat cggataacgt
     7201 gatgccccgg cgcgctcttg cttgaccagg aggaaacggg gggagggctc ttcagcggca
     7261 acctggggaa ccgtcggcca ggagggttcg gtttggatct cgccacccga tgagatcccc
     7321 gcgccgaaca ggacgcaggc gaacgcgagc gtcaggaggt gacggatcaa ggaggcgaca
     7381 tgggacacga accggaactc ctctcgatag gataccctct aggccttttc tcagatgttt
     7441 atggtaatgt caacaaaagg cctcaaacca tttaattgta gatggcaaca catcttcttg
     7501 tcgatgggta taaccttgtg ggcagcgccg gaatggccct cgcggctggt tccggacgcc
     7561 ttgaggcggt gcgcgagacg ctcctgaaga acctggccgg gtaccgtcag agaaaaggcc
     7621 atgcgattac cgtggtcttc gacggctggc agggcgggct gggcgcggaa catcgtgaat
     7681 ttcaatccgg cgtcgaagtc gtctattcaa aacgaggcga acgcgccgac caggtcattc
     7741 aacgtctcgc ccgactgtac ggcagggatt gcgcggtggt gtcctcggat catgaagtgg
     7801 tcaattcagc cagggtggcc ggcgcgttcg tcatcggggc ggcggagttt cgcgccaagt
     7861 tgcaggatcg gtcgtccagc ctgtcgaccg tcgcctttaa agaactggat cgcggcgatc
     7921 tcgatcgcgt gaagcgatcc aaagacaagg gcgggaatcc ccgcaagctg ccgaagtcac
     7981 aacgtaaacg aaaccatcaa cttagggaat tttgaacagg cggcgggcgt tctcgctggt
     8041 acggcgggcc acgtcttcag cggtcgaggg ggcgttgtcg gcggtgatcg tcgccagcag
     8101 ttcggccaca tgcctcacaa aggccggttc gttgcgcttg ccgcgatgcg ggaccggcgt
     8161 gaggtagggg caatcggttt caatgagcag ccgatccgcc gggaccgtct tggcaatctc
     8221 gcgcaacatg gtggcattct gaaaggtcag gatgccggag aatgacagat aaaatcccag
     8281 ctcgatcgca tccttcgcca gccaggcatc tcctgaaaaa cagtgaaaca ccccgccgat
     8341 ttctgaggct cgctcttcct tcagaatgcg aatggtatcg tcctgcgctt ccctggtgtg
     8401 gatgatgacc gggagcttta actcacgtgc cagttgaatt tgctcacgaa agcgcgtccg
     8461 ctgtaattcc ggatcggagt ggttgtaatg gtaatcgagg ccgatctcgc cgtaggccac
     8521 gaccttcttg tcgcgcgcca gccgccggaa ctcctcgtac caaccgtcct cgatgtgttt
     8581 gacttcgtgg gggtggacgc cgatcgacgc gtagacgaag gggtactggt tggcgagggt
     8641 gaccgccgat cggctggtgg caaggtcgca gccgatggtg atcatcgatt ccacgccggc
     8701 cgctcgcgca cgggcgatca tggcctcgcg gtcggattcg taacgcgcat cgtcgagatg
     8761 ggtatgggta tcgatcagca tggggcgcat cctaacatgg ttgtccgctc gtaggatgct
     8821 gaaaaagttt gccggcggcg ctttcaaccg ccgtgaatcg tgaagcgtat ctcggtggga
     8881 ataagagcgt atggctgatg gcgtatagtt ggtagtaaag acgcccactc tctatttcgg
     8941 atcatgtgct atgagctata cgccataggc tcttttctac ggacgagagt cgagatacgc
     9001 ttcacgaacg acggacgaaa gggaaaccat cgggagccac cacccaccag agcgtcccgg
     9061 tatgccaacg tgttcagttc tctcacgcgg atgggatggt cacctcagct gaatgttctc
     9121 gacgatcatc cacaagtcga atcgcatggt cggcctgctg aatgccatcg accatcaccg
     9181 acaccgcctg ctggtccgta cctgattgtc gcaccgtgat gtgatagagg gtctccctga
     9241 atcggtagtg taccttgaac gctttccaat cccgcggaag gcagggaatg aagcgcaacc
     9301 ggttgccttc cagcttcagt cccaggagtg actcgacgat cagccgatac atccacccgg
     9361 ccgagcctgt gtaccaggtc catccgccgc ggccgatgtg aggagagacg gcatagacat
     9421 ctgccgccac cacatacggt tcgactttgt atcggttgat atccccgggt gactgggcat
     9481 ggttcaccgg gttgatcatg cgaaacagtt cccaggcgcg ttcgcggtcg cccagtgcgg
     9541 caaaggccat ggtggcccag agcgcgccat gggtgtattg tcctccattc tctctgactc
     9601 ccggcacgta gcccttgatg tagccgggat tcaacgcgga cttgtcgaac ggcgggtcca
     9661 acagttggat aagggcatcg tctcgccgaa ccagtctccg gtagaccgcc tccatcccaa
     9721 ggcgcgaacg ggttgcctct cccgctgccg acaacacaga ccagctttgc gggatggaat
     9781 cgatttggca ttcgtcgttc gtggccgacc cgagcggcgt gccatcgtcg aagtaggccc
     9841 ggcggtacca ctctccgtcc cagccctgct gttcgatctg ttgctgcagc tgggctgcct
     9901 ctgcctggca gcgctcagcg aacggcacgt caccggtggt tttcgctacc tcagcaaacc
     9961 gcatgaggac gtcatagagg aagaatccca gccagatgct ttctccctgg ccgcgatcgc
    10021 cgaccatatt catcccgtcg ttccaatcac ctgaaccgat gagcggcaga ccgtgtacac
    10081 ccttcgtgag ccccttcagg atggcccgga cacagtggtc gtatagactg cctgattgct
    10141 ccgaccggcc gggaagatcg tagtaggaat cttcgtcggc attgatcggg cgaccttcga
    10201 tgaactggat cggggtatcc agcacgccgg tgtccccggt tgtcatcacg tagcgactgg
    10261 tgacataggg aagccaaaga aagtcatcgg agcatcgcgt gcgcacgccc cggtttgacg
    10321 gtggatgcca ccaatgctgc acgtctcctt ccttgaactg acggccggcg cacagcagca
    10381 gctgttcccg cgcgagatgc ggtttcgcgt ggaccaatgc catcacgtct tgcaactgat
    10441 cgcggaatcc aaacgcgcct cccgattgat agtagccgct gcgcgcccaa agacggcagg
    10501 cgatcgtttg atacagcagc cagccattgg caagcacatt gagcgactga tcgggggtct
    10561 ccacatggac cgctcccaac gtatgtgtcc aatgatgcca gacggtttcc agcgccgcac
    10621 ggcgtgccgt cgcccctctg aagcgacgaa cgagctgacg ggcatcctca gtatcttttc
    10681 caactcccaa ccggaaggta cagtcgcgct cttgaccaac ggcgaggtcg aagggcatct
    10741 gaatggcgcc gcaaggatcc agagcggccc ccactttccc ggacagtcgt gaccgactca
    10801 tggacgcagg attgcgcaag gtgccgttgc gtccgagaaa ttcggttcga tcgccggtca
    10861 tgctccgagt gccctcgtcc acatcaaaga aggcaatccg cccggggaaa tccgtgttgt
    10921 agggatttcg tgcgaacagc gccccggtgg ccgggtctat ttcactgatc acatgcatga
    10981 ccgatttggg gcgcagatca cccagcaccc attccacata gccggtcgcc gacaggcgtc
    11041 gtggtcggcc cgattcgttg cgcagcttca ccaccgtaaa ttttacggag gcatccagcg
    11101 ccacatacac ccagacctct gagcgaatgc cgccttccgt atgctcaaag cagctgtacc
    11161 cgaatccatg ccgactgaca taagggctcg tacccctgca gggcatcggc atcggcgacc
    11221 agacgtggcc gctctcctca tcgcggagat agaaggcttc tccgctcgaa tcggtcaccg
    11281 gatcgttgtg ccagggtgtg agacggaact catgcgcgtt ttcgctccag gtgtaagcga
    11341 ggccgttctc tgagacgacg gtgccgaagt gcgggttcgc cagcacattg acccaggggg
    11401 caggcgttac ctgttcggct gtcgtcgtga tgacgtactc gcggccatcg gaagaaaacc
    11461 cgccgagacc gttatagaac agcagatccg ttcggggctc gatggtggcc ggcggatcgc
    11521 cgcgatgggt acgcgtcggt ttgaagcggt aggccggggc cgcaggggag gcgaggcgtg
    11581 taatctggtc cgccagcgtt ccgcgtcgat cggtgaggat cactcgggcc accgattgct
    11641 gcagcaggcg gtcttcgacc gacatctgtt ccgccgttcg cacgaagata ccgcccggtc
    11701 gatccatcac attggcctcg actccggccg agatcagccc catgatttga tcgtgcaaca
    11761 gctgacgata gccggcgtga tcttcgttcc aaatcacgag gtccacggcc agccccttca
    11821 gtcgccaata ggcatgggcc tggaccattt gacggaccag atcgatgttg gacgggtcgc
    11881 cgatctggag cagtacgatc ggcaggtccc ccgaaatcgc atagccccag aggcctgatt
    11941 gtccgcggcg gtttttgctc agcacggccg cttctgccct gagtgaggca ttcgcataga
    12001 ggatcgagcc ggccagtctg ccatacaatt gcgcatccgc ttcggtcgta ttgagctgac
    12061 gcagcacgac ctggctatgg gtccaggcca gctcaaacac ccgatcggcg agacgccggt
    12121 cctgatactt gtcgacgaga cgcaggcaca ggtcgcggtt ctctccgatg cccgacacca
    12181 gatccacggt gaccgactct tcaggttcga ggacgatcgg acaacggatg gacacaatgg
    12241 ggtccagcac ggagccctga tttcctgaaa gcgggccgga atggtccatg gcctgcggag
    12301 cggcgagggt gcgcccccgg ccgatgaact gcaggcggtc cgtttcgtag gacatctcgc
    12361 cgctgtgagg gccacgtacc gccatgaggt ggaacgccca gggggcatgt tcatcctgcg
    12421 aacggccacg gcgcgtggag agaatggcct gacgttcgcg gatgatttcg gtctggacga
    12481 agagattact gaaggccgga tggagggcat cggccgcggg cagcgccagc acgacttccg
    12541 cataactggt gaggtcgatc atgcgtttcg tgtcggaaca gttggtgaga cggatccgcc
    12601 gtaattcgat gtcgtcttcc ggggacacga cgatttcggc atgtgtctcg atctcatggt
    12661 cgcggcgtcg aaactcggcg cgcccttcgg aaaagatgac ttcgtaggtc ttggacggtt
    12721 tcagtgtcgg ctgataggcc gtggaccaga attccccgct ctctacgtcg cggagataac
    12781 aaaacgtccc ccagttgtcg caggtgctgt cttcacgcca acgcgtgacg gccaggtcct
    12841 tccatcggct ggacccgcct cccgcgttcg tcaccatcac gtggtatcgg ccgttcgaca
    12901 ggaggagcac gtccggcacc ggagtgttcg cggtcgtcag gacgcgcacc ggcgtgtccg
    12961 ccgtgccgga cgtcgtgcgc aaatccgaca gctcggtgct gtgggcgtag aaggctgtgg
    13021 ccttgggaat ccgctcttgg agcaccagcg tgctggcctg aaacagcgga tcggcttcga
    13081 agcgcttctg catcggacgg cccaggagca gataggccaa ggagaggaaa ctcatcccca
    13141 catggtgggc catgtaggag cgaatgatga cacgcgattg tccgcggggc agacgcgagg
    13201 gcgtgtaatc gatcgcttcg tacaagccgt atggtccatc ggcgccttcg gccgtgagtc
    13261 gctggagatt cagacaagcc tcttccggcg cgaccatcag ggcgagggag gtggcgtagg
    13321 gtgcaatgac caattcctcg cccagacctc gcttcagtcc aaggcctggc acaccgaagg
    13381 cccggtactg atagttcaac tgcgcgtcga ccatgttgta gccggattcc gaaacgcccc
    13441 agggaatgtc tcgctcccgg ccgtattcga tctgtcgctt caccgctgcg cggtaggtct
    13501 ggtccaacag cgtctgctgg tacgtcggca tgacgagcag cggcatcagg tattcgaaca
    13561 tcgatccgct ccaggacagg agaatcggct ctcctccagt cgtcgtgagc agacggccga
    13621 gggcgaacca gctctcctgc ggcagttggc cttgcgcaat cgccacgaag ctgcacaatc
    13681 gcgcttcgga ggccaggagg tcgtagtaac tcgcgtcccg tcgtcgttcc gcgacgttgt
    13741 acccgatgga gagaaggaac cgcgtgtcgt cgaacaggaa gtcgtactcc atctgggcaa
    13801 attgattcgc ttgccgggcc aagtggtcga gcgaggccag gcgttgttcg gccagtcggc
    13861 tggccgcgct cagatgccgt cgaagttcga tcagccaggt ccgttccgtg ctggatacgt
    13921 ctggtccaag tcgcgcgtca atcgccggca gccattcgcc ttccagtctg gtcagcgtcc
    13981 ggagcgtcgg gatctgatct aatcctggat acacctggag tatctcatcg gcggcggcca
    14041 gaccgatcca gggagccaga atcgtcagct ctgcgaccgc gttgcggcac tgtctcgcga
    14101 aggcatgggc ccatcggtgc gcctccgcgt tggaggcggg atcgagatgg gcggtgactt
    14161 catccacctg cgtggtgagc agggtgaaag tgtgatgggc ttccgagagt gtggtcggcc
    14221 tgttgtcact ggccgcgagc aggtttttct gaagtgcgtc aacctgagac gtcggagttt
    14281 cgagcgcctc gctcagcagc gcgagggtat cgcgaagtcc ttcaaaggtg cgctcagcga
    14341 ggatcttgtc ttccggcagg ctgaggagcc ctgagtgcag ggtcatgaca tgccctgcca
    14401 gatttccact gtctaccgac gagatgtaca tggggagcag cggtttcagt gtctgggtgt
    14461 cgtaccaatt gtagaaatgg cctcgaaatc gttccaaggt tgccatgctg tcgaaggtat
    14521 gagcggtgcg ttcgatcagc tgtcccgccg acaggtagcc gaagtcgtag gcggagagat
    14581 tggccaacag cgccaaccct atattcgtcg gtgacgttcg atgcgccacc ttcggggccg
    14641 gatgctcctg atagttatcg ggcgggagcc aatgatcctc cgggctgaca taggtctcga
    14701 aaaaggccca ggtcttgcgg gccatcttcc ggagaaacat cgtctggtcg gccgtgagtc
    14761 tggcctcgcg tcgagccagc ggtttgctga tccaccaggt gaacagggga gacagcgccc
    14821 ataggctgag caccggcgcc gcaatcagga gcgcctccgc gcgcgtctgc atcagaatga
    14881 tcgtgatgat cagggccatg gccggaccaa tccacatcga tcggtaagaa ccgatcagat
    14941 ccgtcctgct cctgtccgtt gcgcctgacg ggttccattc caataagccc gtgcgattga
    15001 accatacacg tccggcggtc cgtatgatcg cgtccaggct gtagaagctt tcgtagggca
    15061 ggcacatgag cgtgaggagc gcctgagtga gctgttgtgt catgccccgc gcggttgcgg
    15121 tgaggtgctg gctcatccgc atatcctcgg gcttgcggca cagatcgacg acggcgccca
    15181 gtaacggcgc gatcaggaac aggccgagcg tggcgagggt ccagagccag acaggggaaa
    15241 gcatagtcca tcccgatacg aacaaggagg tcagtgcagc ggggacgaga ctccgtcgaa
    15301 ggttgtcgaa aattttccaa cgcgacaggg tggacagcgg attgctgcgg gtatgtccgt
    15361 tcggtcccgg cacgcggggg aaggcccagc gcgctatttg ccaatctccg cggatccaac
    15421 gataacgccg ggtcacgtcc ctgttgtagc gggcaggata ttcttcgtag agctgcacgt
    15481 cgctcaagag tccggcgcgg gcataggagc cttcaaggag gtcgtggctg aggatgcgat
    15541 tctccgggaa gcgatcgctg agcgcctgct cgaaagcatc gacctcatag atccccttgc
    15601 cgatgaagga cccttctccg aacaggtcct gatacacgtc cgacacggcg cgtgtgtagg
    15661 gatcgatgcc gggttcgccg gcgttgagcc gggcgtaacg cgagcggttg gtgccgggga
    15721 ggctgacggc cacccgaggt tgcagaatcc cgtaccccgc agtcacgcgc tgtaccgcag
    15781 gatcgtactg cgggcgattg agcggatggg ccatggccgc cacgaattgc cgcgcggaat
    15841 cgcgaggcaa ctgggtatcc gtatcgagcg tgatcacata tttcactgtg gacaggatgt
    15901 ccggttgtcc gacgatcgct gaaaaccgat ccgtcgaccc gccacgcagg agcccgttca
    15961 agtccgccag tttgcctcgc ttgcgctcgt atcccatcca gagccgttca tgggcattcc
    16021 agcgacgcgg acgatggaag agaaaaaacg tgtcgccctg ttcacctgca tatttggcat
    16081 tcagcgcatc gatgcctacc ccggccgcca gcaccaactg atcgtcacct gccaccgtct
    16141 cctggtcggc atcctggaaa tccgtgagca gggcaaagtg tagatgactg ccccggttgg
    16201 cgaggaatcg gacctcgagg gcctcaagga ggtcgtcgat accccggaca tcttggagga
    16261 gtgtgggaat cacaaccagc gttcgtaacg ccgggggaag gccatcggaa aagtccattc
    16321 gcgggagggg atgtggggtc accagcgccg tggcgatcca gttcacgagg gtcaccgcca
    16381 gctggctggc agccaaggcc gacacgaatc ccagcagggc aagcggccat ccattccaac
    16441 ctgctgcaat ggccagggcc aggaaacatc cagtgaatgt ggctgtgagg aacaggatgg
    16501 agccgagata gattggtacg ctgctggatc cggtccatct ccggagcctg tcgaaggcag
    16561 aacggcgcag gctcacggcc tgttccaatt gcgtctgtcc gttgtcgatg agatagaagc
    16621 cgacgtgggc ggtgcggtgg tccggtccct ctgcagcggc ccctgtctgt gcgagcagga
    16681 tcgcctggcg agcgacgtca ccttccgaca gggggctact ctttgcgagt ttctccacag
    16741 catggcgata ccggtcacga gtggcaaaat ccatctgccc gtacacgccg gccggatctt
    16801 catggaacgt ctgctccacc aggctctgcg cttcgacaaa ctcgcgccag tccatcgccg
    16861 ccaggaaccg gagactgccg atgctgttgc tcatggagac ttgatcgatc gcctgttgtt
    16921 gcgtctccga ctgcaccagt tgctcgatcg tgaggccgga ctcggacagg cgctgttcga
    16981 tccaggtgag cggtaaggcc aacgccgcgc tctgtccttg cagacggcgg gcgagctctg
    17041 ccacgaaggc gctcaccatg ggcggattgg accgcgccat gtccgcgatc accaggatga
    17101 gactcttggg gtccttttca gccgtctcca tcatttggcc ggcccattcg tgggcgcgat
    17161 tgcgatccac tcggtcgctt gcgatgcggg cgccggcgcg tcgcaggttt tcgatcaatg
    17221 cgaggcgaat catgataggg atggcccaca actcgccgag cttcagggtc gtgaccgact
    17281 gataggccgc gacgaaaccg ctgagacttt ccgggtccac ccgtccgtcg ccatgcgaga
    17341 tgatttcaag cgcgatgtca tagaccctgg gcaagccggc cgatggaccg ttgcccagcc
    17401 ggggcagttc gcggctatac cctttgggca ggtgccgctt ggccaggcga atctgttctt
    17461 cgatcaggta aaagttatcg agcaaccatt ctccggccgg tgtgatgcgt cggtttccct
    17521 tcactgcctc ggccaccaga ttccgcacat ccagcaggac gacttcattc tcggccagtc
    17581 gagcgagcag cgggtcgggc tcgtgctcgg cactgacggc atgcaacccc gcgagggttt
    17641 tgccatgctg ttgcatttgc tcggcgctga acagctccga tcgcaacggc ggctcatcac
    17701 cggcataggg catggtcgcc ggttgtccgc tcaggcgtcc ggtcaattga cgccagcttg
    17761 caagaatatc tgtgcggctc atcttcaaat tcaagattcc cttcgatcga ggtcgcggag
    17821 gtggtctgcg ggatacaggc aaaggaccgg cccaagccgg acggtccttg cgaatgaagg
    17881 tacggaagcg ggcacaagga ggcagtagag ccccacccgc atgattcata acggcttctg
    17941 ctgcaatcgc cgatctgtcg ctgcgacaga ccttcgtccg gcggactcgc ccctcacccc
    18001 ctcactgcac tgcgcgagcg ggcccgtggt cttgggactc cgtacgcctg gcgcagtgcc
    18061 gctcagtgac tttgcggcag accgtcatga atcatgcggg ccgaggaatc tgcaagcgaa
    18121 cggcgattgt gcaatcgaca attcggacac ttcgatgcga gctgtgacgc ggatcgaacg
    18181 cagcgatggg aaggtgtgct ggatgctgtt ctactctagt ccttttaggc aggtgaaaga
    18241 cagacaaata tcctcaggct gtgcgggctg gagcgtcgaa gggagttctt gaggtgcgcg
    18301 acgagcacgg gagaaggctg agcagccggc gctcagtcag actcactggt tgtagtcata
    18361 gcgtgggggg gcgctcctca gccgaggagc gagtcgtcga aacgaatcag tcggtgagct
    18421 ggtgggagtg tgcacgcaaa aaatgttgca cagggcctcc gatcgaacat gagcagcctg
    18481 caggctgttg agaaagcctg tccggcaagg ccgcagcgag cgaagaggtg gaatcgtact
    18541 cgcgccgtac ggtgagcctc tgagcgaagc gagaacgccg ctggcgggcg tggtcagtag
    18601 cctgctagac gggtcgcgtg gcgagcgact cagccatgcc ataaagcaac gcttcggtgt
    18661 cgctccaggc caggcaggaa tcggtgatgg acatgccgta ggtcagggcc ttgttgggat
    18721 cccaggtctg ccggccgcct tgcaggtggc tttccagcat gaggcccatg atggcacggc
    18781 ggccttgctg gaactgtttg aggacttcgc cggcgacttc cacctggcgt tgatggttct
    18841 tgccggaatt gtcgtgcgaa caatccacca tcacgccgcg agccagacct tcgctggcta
    18901 cggccgcctc cgcgcgggcg atatcttcga cactgtagtt ggtgcgtccg ccgccgccgc
    18961 gcagcacgat gtgatgatcg gggttgccgg tcgtcttgat gatagaggtg atgccgtcgg
    19021 cattcacgcc gacaaaatgg tgcgggctcc ggctggtgat catggcgttg atggcgactt
    19081 gcaggctgcc ttccgtcccg ttcttgaatc cgacgggcat ggagagaccg ctggccattt
    19141 cgcgatggat ctggctctca gtcgtgcgcg cgccgatcgc ggtccagctc aacaggtcgg
    19201 cgatgtactg cggggtgacc gggtcgagca gttccgtcgc gcaggggaga cctttgccgt
    19261 tgatgttcaa gagaattgtc cgggccagct ggataccttg ggcaatgtcg caggtgccgt
    19321 ccagatgggg atcgttgatc aggcccttcc agcccaccgt ggtgcggggc ttctcgaaat
    19381 aggtgcgcat gacaatcagg agtttgtcgc ggacggcgtc ggcgaccggc ttgaggcggt
    19441 cggcatattc ataggcggcg tcgggatcat gaatcgagca ggggccgaca atcacaatga
    19501 gccggtccat gtcacggccg tgcaggatgt tccggatcgc ttgtcgcgtc tgaaacacga
    19561 gctcggacac ctcgtcgctg ataggcagct gcgttttgac atcgcggggc gaggggagcg
    19621 gtttaatctc tatgacatgc tgattatcga tcggcctgtt catgcttatc cctttgaaag
    19681 gtctgtctag ttcacgcgtc gattcaagat tgccgaataa gatatcgaat tctataggat
    19741 aaagtccagt atggacggtt gtaggaggat cggcttgcat tcgagggggg ccctttgtta
    19801 caactcacgc ctcacgaatc tgccggcccg gacggtcatg tttcgtcatt tcgcccagaa
    19861 aaaatccagc tcggtc
//
LOCUS       DAORQN010000019        18737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_196_length_18737_cov_20.025693, whole genome shotgun sequence.
ACCESSION   DAORQN010000019 DAORQN010000000
VERSION     DAORQN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18737
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_18737_cov_20.025693"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>833
                     /locus_tag="PK614_02590"
     CDS             60..>833
                     /locus_tag="PK614_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB81129.1"
                     /translation="MDSSFTLTIDWLAFTVLASNPQETMKVLGGDWSKAKGGFRGYPL
                     SWMRADGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRSLLKWVHAQQGHVTR
                     IDCALDDRAGTVPVSTIREAVSAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELQSKEQPNWQEYGVRWELELKKDRAEQCARALATLEEADWKELVI
                     GLLRSYVDFRQIPKDAEDEERYRAPVLEWYALLTEGFQKG"
     assembly_gap    834..1015
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1095..1409
                     /locus_tag="PK614_02595"
     CDS             1095..1409
                     /locus_tag="PK614_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81130.1"
                     /translation="MLTVKELSAWLNIKPSTLYLWVSQNKIPCRRIHGLVRFEPEDIR
                     KWLNAFESATASRLPALRRDDTRDLDHLIEAAKCEVYTPRHGETRPKSGLIQKEDADG
                     AV"
     gene            1396..2472
                     /locus_tag="PK614_02600"
     CDS             1396..2472
                     /locus_tag="PK614_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB81131.1"
                     /translation="MGLFKRNKVWWMTFVYQGRQVRRSTECTDRRLAEAVLGKIKVKL
                     VEGRYFDRLEEQERTFEEMVERYVAERVVGASRHGERRARAVLAHLLPVFGKKTLVQV
                     TPKEIAAYRWKRQQAGAAPATIVKELALMKTAFNIAIREWEWCRDNPVCRVSMGKVNN
                     ARVRYCDDETLARIYQACPTWLQPIVMLARYTGLRRENVVCLQWEQVDMARGLILLDH
                     TKNGDRLGIPLCDPALRTLQAVRPSRPLASGSVFLQHTEDREPVTPDMVTTAFRRACE
                     SVGVTNFRFHDLRHTFASALVQKGVDLYRVQRLLGHRDGRMTQRYAHLAPENLREAVQ
                     VFKDDYHKFSTMPGVGPTRLVLTA"
     gene            complement(2479..2556)
                     /locus_tag="PK614_02605"
     tRNA            complement(2479..2556)
                     /locus_tag="PK614_02605"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2518..2520),aa:Glu,seq:ctc)
     gene            2729..3163
                     /locus_tag="PK614_02610"
     CDS             2729..3163
                     /locus_tag="PK614_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81132.1"
                     /translation="MVLCSTRLRTFLLAGLLPAFLSLAGCGTSFLEGTIAPPYSSAWT
                     KCGATTKVRVKPPHSTVTVAYTEPTTGTDGKPLTNLAYTTIYYNAGDGPVIGKVVPAS
                     GKTGGAAVSQVVMIPLRDQSEQEVTVCVTATDSDEREGPASP"
     gene            3288..3812
                     /locus_tag="PK614_02615"
     CDS             3288..3812
                     /locus_tag="PK614_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81133.1"
                     /translation="MELRKPDPAHSSHEAESDRHKHSLEIRLGSNIFRNTNGVIRVQG
                     KEQLVLELAPDQERILLTIDLYDGSGNHVAHLRRNRWAFNDGNRFSLNTSESPPTLFP
                     NLPWLKVTDQETGETVLEAAVAPGEKIHVATGKFYSHRGQLIEITSHFCRIGSTHTLF
                     GDVFEARGGTAVLG"
     gene            3852..4241
                     /locus_tag="PK614_02620"
     CDS             3852..4241
                     /locus_tag="PK614_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81134.1"
                     /translation="MQAFLVCDCVIEDSLTKKKSLIGLFTHLQSVTFPFQHHQLGLYF
                     CITDAEGTYHVEIDLMFVNTDQLVCRASLPDIVIGDRLQISDFGINIPALLFPAPGRY
                     EFRLRMNGRMIAQKDFHVVQVSAPPGT"
     gene            complement(4477..5658)
                     /locus_tag="PK614_02625"
     CDS             complement(4477..5658)
                     /locus_tag="PK614_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81135.1"
                     /translation="MPGNGEEEMMNGRWSAWGLVVVLGILWVGGSQAAEPDSLEACRA
                     QPFDAKMQCYQRHLETVLQAEGTEQALTVLEQITVQDPEALREAHPLVHHVGQRSFAR
                     YGTAPLALAHCRDVFWSGCYHGVLQAFLSSLATVEPQHILPLCPISETVSAYSFQRYN
                     CLHGLGHGLTIQFRYDLLKSLAFCDALPGSWDRESCYGGVFMENIVTFQQAQRAQPAQ
                     SGSEHHHHEATSFLNPQDLLYPCSVLNEKYLRACYLMQTSAVLTFLNYDFAQAFTQCA
                     RVEGEHQTTCYRSLGRDISGYTLRDAVRVNELCRLGQAEQIRQCFIGAVKDFILTDAN
                     PDPGLALCRSLDGPFKKDCYATVGEMVIPLFDDKEKRAQACRKGEDEYVDACLAVATA
                     F"
     gene            5833..7536
                     /locus_tag="PK614_02630"
     CDS             5833..7536
                     /locus_tag="PK614_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRB81136.1"
                     /translation="MSTTPESPTRSDFIREIVAADRAAGKHEGRVVTRFPPEPNGYLH
                     IGHAKSICLNFGIANENSGGICHLRMDDTNPTTEDPEYVQAIQDDVRWLGFDWQDKMF
                     FASDYFERLYDYAVGLIQNGLAYVDSLTADDMRKYRGTLTEPGQASPFRTRSVEENLD
                     LFRRMRAGEFPDGTHVLRAKIDMASPNINLRDPVLYRIRHVAHYRTGTAWCIYPAYDF
                     AHPLSDAMEGITHSICTLEFEDHRPLYDWVVEHCGTAQRPQQIEFARLNVTFTVMSKR
                     KLLDLVERKLVNGWDDPRLPTLKGLRRRGFTPEALRAFCEAIGVAKRDAVVEMQLLEH
                     FVREDLNKRAPRVMAVLRPLRLVIENYPEGQVEQLEAVNNPEDPSAGTRQVPFSRTLY
                     IDQEDFREDPPKQFFRLSPGREVRLRYAYIITCVGVVKDPATGEVTELRCTYDPETKS
                     GGAQAQRKVKATIHWVSASHAVDAEVRLYESLMLMDPGKIPADQDWTQHLNPHSLERL
                     PTCKVEPSLAAIAPGTRVQFERVGYFCADPDSSPDKLVFNRTVTLKDTWAKIENAQTP
                     R"
     gene            7688..8023
                     /locus_tag="PK614_02635"
     CDS             7688..8023
                     /locus_tag="PK614_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81137.1"
                     /translation="MVTGPCSEEHLEAQGSFRERLSRLEAAIPGAAAEAQPLLRHIYR
                     ATRRLHDQLQRIDPTPLLPRSAVSEVRCFDCGTARMESFHTSTQGGYHLCPTCFQQRL
                     RTGRARTAH"
     gene            8168..10012
                     /locus_tag="PK614_02640"
     CDS             8168..10012
                     /locus_tag="PK614_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRB81138.1"
                     /translation="MAPNILLSCESISKSYGVRALFTDLSLALSDGDHVGLIGPNGSG
                     KSTLLKILVGLESPDEGTRTLRRHTRVGYVPQEPLFPPHHSIEQVLQGTLTEDGLDPH
                     EQGGRIARALSIGTFPDPEQAVSTLSGGWRKRLAITQALLLEPDVLLMDEPTNHLDLE
                     GIIWLEQLLKNRAKAFLVISHDRRFLESVTSRMVELNRCYPEGRFESRGCYSDFLEQR
                     DAALQAQADYQASLANRVRREVEWLRRGPKARTTKAKGRIQSAGKLIDELNQAESRSA
                     QSTIGIDFSASGRKSKQLVVAEQASKSFNGTPVITNLDLLLGPGQRLGLLGPNGSGKS
                     TLLRLLAGTLEPDSGTITRADALRIVSFEQHRESLDQSTSLRRALAPSGDAVVYQDRS
                     IHLASWAKRFLFRPEQLDLPVSKLSGGEQARLLIARLMLQPADLLILDEPTNDLDIPT
                     LEVLEDSLLEFPGALVLVTHDRWLLDRVSTILLALDGTGRVDWFADYAQWESAQERAG
                     GSAPESKSDGRAAVQDETASATPARKPKKMSYREQKEWGTIEESILKAEEEVTACHAA
                     MQDPAVLSDAAALQARSQALVAAQAEVERLYARWAELDEKRAQTVQSS"
     gene            complement(10107..10180)
                     /locus_tag="PK614_02645"
     tRNA            complement(10107..10180)
                     /locus_tag="PK614_02645"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10146..10148),aa:Gln,seq:ttg)
     gene            complement(10234..10307)
                     /locus_tag="PK614_02650"
     tRNA            complement(10234..10307)
                     /locus_tag="PK614_02650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10273..10275),aa:Gln,seq:ctg)
     gene            complement(10378..11793)
                     /gene="gltX"
                     /locus_tag="PK614_02655"
     CDS             complement(10378..11793)
                     /gene="gltX"
                     /locus_tag="PK614_02655"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRB81139.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQKGVFVLRIED
                     TDQSRSTDDSIQAILDGMRWVKLDWDEGPFRQTERMDLYREHAMRLFEQGHAYWCVCT
                     SEELEARRKEAETKGGSPKYDGRCRNLGLTKPTGEAALRFRAPQDGQTVVDDLIKGPV
                     VFDNQILDDVIILRSNGYPTYNFSVVVDDALMGITHVVRGDDHLTNTPRQIPIFQALG
                     FPLPRFGHLPMILGPDKARLSKRHGATSIMAYKDMGYLPDAMVNYLVRLGWSHGDQEL
                     FSRAELIEKFSWKNVQTSAAVFNPDKLLWLNAEYLKTSPPAEIAQALVPLLEQAGLKE
                     EVRAVSTEWLAQLVVLVKERTKTLVEMVHWVTPYFGQAAAFDEEAAKKFLTATQAPVL
                     AKLLSRFEAFPTFSKQEWEESFKQLVEEEGIKMGQLAQPVRVALTGRTASPGLFEVMD
                     VLGRDRTLFRLRQGIERASRS"
     gene            11911..12609
                     /locus_tag="PK614_02660"
     CDS             11911..12609
                     /locus_tag="PK614_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB81140.1"
                     /translation="MAEPTQIAEIVSLTTLTPHTTELVLRPIEHPLSFKPGQWISLQL
                     PVGDHPPLNRAYSLAEPPSTAGHLTLLFDHVPGGKGSGYLASLKPGDRIPLSGPYGHF
                     VLPDSEPQHLLLIGRYSGLVPLRCMLRALAGQGLLPSSTLIAQAPTTAEQLYHEEFMA
                     LAASQSNFRYLPFVSEGPESPTETVDAIKQIAGNGRQVTPMIAGVKAFARPLRALLME
                     LGFDRREVKLETYD"
     gene            complement(12648..13064)
                     /locus_tag="PK614_02665"
     CDS             complement(12648..13064)
                     /locus_tag="PK614_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB81141.1"
                     /translation="MGGTNPYIEKADVELPQKSYIVTFIFPDKTEKKVEVQPDKIPYG
                     PTGLPGSILDIAMGNGIDLEHVCGGVCACSTCHVMVKKGLETCNEGTDDEFDQLDEAP
                     VTTLQSRLGCQCVPNGTVEVVVEIPAINKNLAKEGH"
     gene            13271..13750
                     /locus_tag="PK614_02670"
     CDS             13271..13750
                     /locus_tag="PK614_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRB81142.1"
                     /translation="MLKLSKKADYGLMALQHIASNQYGDVAQARVVNTKEIAEEYHIP
                     VELLAKVLQTLSKSGIIESHNGPKGGYLLGKNPREITIAHVLESLEGPLGIMDCSHEK
                     DGDACMQREHCNIRTPLLKIQSSIYQLLNNMTLQDMMGGTPLITIQSVATEQQGVER"
     gene            13747..14964
                     /locus_tag="PK614_02675"
     CDS             13747..14964
                     /locus_tag="PK614_02675"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRB81143.1"
                     /translation="MKFPIYLDNHSTTPMDPRVLESMLPYFTEKFGNAASRNHAFGWD
                     AEEGVEAARKQIAKLIHADAKEIVFTSGATESNNLALKGVVEMYHEKGDHIITSSTEH
                     RAVLDTAKTLEAKRGVKVTYLPVDKFGMVNPEDVRNAITDKTILISVMFANNEIGTIN
                     PVKAIGKIAKEKGILFHCDATQGVGKVPIDVQDMGIDLMSFSAHKIYGPKGVGALYVR
                     KKNPRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMSADGARLSVMRDR
                     LHATITKALEEVYLNGHPTERLPHNLNISFAYVEGESLLMGCKEIALSSGSACTSATL
                     EPSYVLRALGVGAELAHSSIRFGLGRFTLDEEVDYAGKRIIEVVTKLREMSPLYEMAK
                     EGIDLKSVSWAAH"
     gene            15007..15390
                     /gene="iscU"
                     /locus_tag="PK614_02680"
     CDS             15007..15390
                     /gene="iscU"
                     /locus_tag="PK614_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRB81144.1"
                     /translation="MAYSEKVVDHFNNPRNVGSFKKEEDGVGTGIVGAPECGDVMKLQ
                     IKVENDTIVDAKFKTFGCGSAIASSSLATEWLKGKTVEEAGKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADSK"
     gene            15410..15769
                     /locus_tag="PK614_02685"
     CDS             15410..15769
                     /locus_tag="PK614_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRB81145.1"
                     /translation="MDATTNTDAQAPVITLSDAALKEVKRLINVQGIEEGGLRLGVKG
                     GGCSGLSYTINFDEKIGQYDQVYEFDGVKVIVDAKSAIYLQGTQLDFQKDLMGGNFKF
                     VNPNANKTCGCGESFSA"
     gene            15784..16500
                     /gene="hscB"
                     /locus_tag="PK614_02690"
     CDS             15784..16500
                     /gene="hscB"
                     /locus_tag="PK614_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRB81146.1"
                     /translation="MSGHIHNTDDRRELQMARSMCWHCQSEMAGEYFCDRCVKVQPVS
                     KDLDYFTCFGLPRRLVIDPQTLETKFYELSRAFHPDFYQNKSDAEQTISLGNSAMLNT
                     AYRTLRDPIQRAEYLLDLEAGSVKDIRTTPPADLFEEILELQDTLDEFRASDRASESA
                     STLRTKLHADRATLEQRQRDMEAQLQQLFSRWDALQDRGEATEQARAERGRILKEMRD
                     ILSNRTYVNNIVNDLVATIA"
     gene            16506..18332
                     /gene="dnaK"
                     /locus_tag="PK614_02695"
     CDS             16506..18332
                     /gene="dnaK"
                     /locus_tag="PK614_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRB81147.1"
                     /translation="MARIVGIDLGTTNSLVAYMDHGTPRVISARHERAMVPSVVALTD
                     NGLIVGDPAKEHLTRNPERTVYSVKRFMGKSLADVQNELAYFPYTLTEKGGVIRIKLG
                     EKSYSPPQISAMILKELKLRAEAFLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQEKTQGTIAVYDLGGGTFDISILKLKNGIFEVLATNGDTHL
                     GGDDFDQLIADLFLADIRTQYGLDLSTYSDHMQAVRLEAERAKIRLSEELKTAVSLDL
                     PESKGRFTRELTRDQLEGLCTALVERTLGPCRLALKDSGLTPGDIDEVVLVGGSTRMP
                     LVRQRVQALFGKRPHCELNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASAKEMFTTYVDGQTGVDIHILQGERELAKDNRSLARFRLKVPP
                     LPAGVPRIEVTFLIDANGILNVMAKDMRTGETQSIEVKPSYGLSDQEVERMIEDSFKF
                     AADDVNARKLIEARLDAGALITTTEKSLADGGHLVATGDVEAIRTALSALATAKDGSD
                     PRAIRARMADIEQAAKGLTVAMLDDSLKQGLQGKKVSDVTQS"
     gene            18348..18560
                     /gene="iscX"
                     /locus_tag="PK614_02700"
     CDS             18348..18560
                     /gene="iscX"
                     /locus_tag="PK614_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRB81148.1"
                     /translation="MDLKWSDTEELAIRLVEAHPTTDPLTVRFTDMHTWIVALPEFKD
                     DPKKSNEKTLESIQMAWHEEYQDSQS"
BASE COUNT         4178 a         5625 c         5125 g         3627 t
ORIGIN      
        1 cgtcttggta acacgggggc gctacccaca gcgctccgta caggagttcg ataggttcca
       61 tggattctag cttcaccctc accattgatt ggctcgcctt tacggtccta gccagcaatc
      121 cgcaggagac catgaaggtc ctcggcggcg attggagcaa ggctaagggt ggcttccgag
      181 ggtatcctct gtcttggatg agggcagacg gtctgcgcgg agtcggcaaa ttgggcacca
      241 atgcccctcg tcggccgaat gaaatccatg tggatctgtc gggcggccta gcgtccgccc
      301 tgacactgga tcagatccga agcctgctca agtgggtgca tgcacagcaa gggcatgtga
      361 cgcgcattga ttgcgcgctg gacgaccgag caggcacggt accggtctcg accatccgag
      421 aagcggtttc ggcaggtcag tgcgtcacga gggcggctca ggtccggcat atcgtgtcca
      481 acctcacgca tgggacagga gcgacgactg gcgagacgat gtacttcggg agtccgcaga
      541 gccagacctt gttgcgtatc tatgacaagc gattagagct ccagagcaaa gaacagccga
      601 attggcagga gtatggagtc cgatgggaat tggagctcaa gaaagaccgt gcggaacagt
      661 gtgcccgagc attggcgact ttagaagaag ccgactggaa ggagttggtg atcggcttgc
      721 ttcgttcgta tgtcgatttc cggcagattc cgaaagatgc cgaggatgaa gaacgatacc
      781 gggctccagt gttggagtgg tacgccctcc tgacggaggg atttcagaag gggnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggt
     1021 ctgccggcgg tcacgcgggc agcccatgtt agggggactg ggggtgtcga gagactcccc
     1081 cggtgatctg taccatgctt accgtcaaag agctttcggc ttggctcaat atcaagccgt
     1141 ccacactcta tctttgggtc tcacagaata agattccctg ccgccgtatt catggcctcg
     1201 tccgctttga acctgaagac attcggaagt ggctcaatgc ctttgaatcg gccacagcaa
     1261 gccgcttacc tgccctcaga cgcgacgata cccgggacct agaccatctc attgaagcgg
     1321 cgaaatgtga ggtctatact ccccggcacg gggaaactag accgaaatca ggcctcatcc
     1381 agaaggagga tgcagatggg gctgtttaag cgcaacaaag tgtggtggat gacgttcgtg
     1441 tatcaggggc ggcaggtccg acgaagtacc gaatgtacgg accgccgact cgccgaagcg
     1501 gtgctcggaa agatcaaagt caagttggtc gaaggtcgct atttcgaccg actcgaagaa
     1561 caagaacgga cctttgaaga aatggtcgag cgttacgtgg ctgagcgagt agtgggagct
     1621 agtcgtcatg gcgaacgacg ggcgcgagcc gtcctggctc accttctccc ggtgtttggc
     1681 aaaaagacct tagttcaggt cacgccgaag gagatcgccg catacaggtg gaaacggcaa
     1741 caagcaggag cagccccggc gaccatcgtg aaggaactgg cgttgatgaa gactgctttc
     1801 aatatcgcca ttcgagagtg ggaatggtgc cgggacaatc cggtgtgccg ggtgtcgatg
     1861 ggcaaggtga acaatgcacg cgtccgctac tgcgatgacg agacgttggc gagaatttac
     1921 caggcctgtc ccacctggct gcaaccgatt gtgatgctgg cacgctacac cggcctgcgc
     1981 cgggaaaacg tggtgtgttt gcagtgggag caagtcgata tggcccgggg actgatccta
     2041 ttggatcaca ccaagaacgg ggatcgttta ggcattcctc tgtgtgatcc tgcgttgaga
     2101 accttgcaag ccgtgagacc ctcccgacca ctcgcaagcg gctcggtgtt cctgcaacac
     2161 accgaggata gggaaccggt gacgccggac atggtgacaa cggcctttcg acgcgcctgc
     2221 gagtccgtag gggtgacgaa ctttcgcttt cacgacctgc ggcatacctt cgcttcagca
     2281 ctcgtacaga agggcgtgga tctctatcgg gtgcagcggt tgctcgggca tcgcgatgga
     2341 cggatgacgc aacgctatgc gcatctggcg ccggagaatt tgcgggaagc ggtgcaggtg
     2401 tttaaggacg actatcacaa atttagcaca atgccggggg tgggtccgac ccggcttgtg
     2461 ctaactgcgt gaaatggttg gtggtcccaa cgggatttga acccgtgtct gagccttgag
     2521 agggctccgt cctaggccaa gctagacgat gggaccagag aggcaatctg cgaggcagct
     2581 gaaagagcag gactgtacca tagcgggttt tggcttttca aacagcaagt caaacaggag
     2641 gtggccgagg cttgctgaac agagaatcga gaccgaattg ggctcagcct gcttgcggca
     2701 gcctatcgaa ttggcctaaa gtagcactat ggttctatgc tccactcgcc tccgaacatt
     2761 tttgctcgcg ggcctactcc cggcgttcct ctcactggcc ggctgcggga cgagctttct
     2821 ggaaggaacc atcgcgcccc cttattcaag cgcttggacc aagtgcgggg ccaccaccaa
     2881 ggtccgcgtg aagccgccac attcgaccgt cacggtcgcc tacaccgaac ccacgaccgg
     2941 caccgacggc aaacccttga ccaaccttgc ctatacaacc atttactata acgccgggga
     3001 tggcccggtc atcggcaagg tcgtccccgc gtctggaaag accgggggag cggctgtgtc
     3061 ccaagtcgtc atgattccgc tgcgtgacca atcggaacag gaagtcaccg tctgtgtgac
     3121 cgccaccgat tccgacgagc gcgaaggccc cgcctctccc tagcccgacc agaacggact
     3181 tgacagcgcc ccgccaaggg cctaccttgc tcccgacttg cccaggcaag aacgtatggt
     3241 cgagtgcgtc gctcaacagg tcctcgggtc gccctagatc tctacgcatg gaactacgca
     3301 aacctgaccc agcacactcc tcccacgaag ccgaatcgga tcggcacaag cacagcctgg
     3361 aaatccggct cggttccaac attttccgga ataccaacgg agtcatccgg gttcaaggca
     3421 aagagcagct cgtgctggag cttgcgccgg atcaggagcg catcctgctg accatcgacc
     3481 tctatgacgg cagcggcaat catgtggccc atctgcgtcg caatcgctgg gcgttcaacg
     3541 acggcaatcg cttttcactc aacaccagcg agtccccgcc gaccctcttt cccaatctgc
     3601 catggctcaa agtcacggac caggaaaccg gcgaaacggt cctggaagcc gctgtcgcac
     3661 caggtgaaaa aatccacgtg gcgaccggga aattctattc gcaccgcggc cagctcatcg
     3721 agatcacgtc gcacttctgt cgcatcggat ccacccatac gctgttcggg gatgtcttcg
     3781 aggcccgcgg cggcaccgcg gtgctcggct gacaatggcc agatcgcatg actgacgtct
     3841 cgccgcccag cgtgcaggcg ttcctcgtct gcgactgcgt catcgaagat agcctgacta
     3901 agaaaaaatc tctgatcggc ctgttcaccc acctccagtc ggtgacgttc ccgtttcagc
     3961 accatcagct cggcctgtac ttctgcatca ccgacgccga aggcacgtat cacgtcgaga
     4021 tcgatctgat gttcgtcaat acagaccagt tggtctgccg cgcatcgctc cccgacatcg
     4081 tcattggaga tcgtctgcag atttccgact tcggtatcaa catcccggcc ctgctcttcc
     4141 ctgcgcccgg tcgctatgaa tttcgtctcc gcatgaacgg ccgcatgatc gcgcaaaagg
     4201 attttcatgt agtccaggtg tccgccccgc ccggcacctg acctcgaacg gattccctgc
     4261 gaatccttcg gcgaccgtga agcgtcgttc gtatgcggtt gaagaaccga gcataggaac
     4321 tgaaatggag ggcgtggggc gtggctacca gcgataagcc atgagccata cgcgcttatc
     4381 ctcaccgaga tgcgcttcgc gctttacgag acacggttct gatcaggggc aggatccgag
     4441 cggcgtgact ggaccgggag ttccgaaaga cccccgctaa aaggcagtgg cgactgcaag
     4501 gcaggcatca acatactcgt cttcgccttt gcggcaggct tgcgcccgct tctccttgtc
     4561 gtcgaagaga ggaatcacca tttcacccac ggtggcataa cagtcttttt tgaacggccc
     4621 gtcgaggctt cggcagagcg cgagccccgg atccggattg gcgtcggtga gaatgaagtc
     4681 tttcacggcg ccgatgaaac attgccggat ttgctcggcc tgtcccagcc ggcagagctc
     4741 gttcacccgc accgcatcac gcaacgtgta gccgctgatg tcacgaccta aacttcgata
     4801 gcaggtcgtc tgatgctccc cctccacgcg agcacactgc gtgaacgcct gcgcaaaatc
     4861 gtaattcagg aacgtcagca cggcggaggt ctgcattaga taacaggccc gcaggtactt
     4921 ctcattcaat acggagcagg gatagaggag gtcttgaggg ttgagaaaac tcgtcgcctc
     4981 atggtgatga tgttcgctgc ccgattgcgc cggctgcgcc cgttgtgcct gctggaacgt
     5041 cacaatgttt tccatgaaga cgccgccata gcaggactcc cgatcccaac ttcccggcaa
     5101 cgcatcacaa aaagccagac tcttcagcag atcgtagcga aattgaatgg tcaggccatg
     5161 gcccaaccca tggagacagt tgtaccgttg aaacgaatag gcggagaccg tctcgctgat
     5221 cgggcacagg ggcagaatat gctgtggctc gacagtcgca aggctgctga ggaaggcctg
     5281 caagaccccg tggtagcagc ccgaccaaaa cacgtctcga cagtgggcca gcgcgagcgg
     5341 tgccgtacca tagcgcgcaa acgaccgctg gccgacgtga tgaaccagcg gatgcgcctc
     5401 acgcaatgcc tcaggatcct gaactgtgat ctgctccaag accgtgagcg cctgttctgt
     5461 gccctctgcc tgaaggactg tttccaagtg ccgttgataa cactgcatct tggcgtcgaa
     5521 cggttgggcc cggcacgcct cgagtgagtc gggctccgcc gcctgacttc cgcccaccca
     5581 gaggataccg agcaccacca ccagtcccca tgcgctccat cgaccattca tcatctcttc
     5641 ttctccgtta cccggcatcc gccagacatc cgccttccac agcagcctga ttttaaaata
     5701 gtccttccga tcgatccagg aaaaatcgac tcaccacagc ctgagacgcc gggccctctt
     5761 ctcccacccc gccgttctgt gatagaacca tgcgcgtttc atcacctgca actcgacgga
     5821 ctcgcaatca ttatgtccac cacgccagaa tcaccgacac gttcagactt catccgagag
     5881 attgtcgcgg ccgatcgtgc tgccggcaaa catgaaggcc gagtggtcac ccgctttccg
     5941 ccggagccga acgggtatct ccatatcggc catgccaaat cgatttgcct caacttcggc
     6001 atcgccaatg aaaattccgg cggcatttgc catctccgca tggatgatac caacccgacc
     6061 acggaagatc ctgaatatgt gcaggccatt caagacgatg tccgctggct cggtttcgac
     6121 tggcaggaca agatgttctt cgcgtccgat tactttgaac gtctctacga ttacgccgtg
     6181 ggcctgattc agaacggtct cgcatacgtc gacagcctca cggccgacga catgcgcaaa
     6241 taccgcggga ccctgaccga gccgggccaa gccagtccat tccgcacccg gagcgtcgaa
     6301 gaaaacctgg atctgtttcg gcggatgcgt gccggtgaat ttcccgacgg gacccacgtg
     6361 ctgagggcca agatcgacat ggcctcgccg aacatcaatc tccgcgatcc cgtgctctac
     6421 cggatcagac acgtggccca ttaccgcacc ggcacagcct ggtgcatcta cccggcctac
     6481 gacttcgcgc atcccctctc cgacgcgatg gaaggcatca cccattcgat ctgcacgctg
     6541 gaatttgaag accaccggcc gctgtatgac tgggtggtcg aacactgcgg cactgcacag
     6601 cggccgcagc agatcgagtt cgcccggctc aacgtcacct tcaccgtcat gagcaaacga
     6661 aaattgctcg atctggtgga acgcaaactg gtgaacggtt gggatgatcc tcgtcttccc
     6721 acactgaaag gcctccgccg ccgcggtttc acgcccgagg ccctgcgggc cttttgcgag
     6781 gccatcggcg tcgcgaaacg ggatgccgtg gtcgagatgc agctgcttga acacttcgtc
     6841 cgggaagact taaacaaacg tgcgccccgt gtgatggcgg tgcttcggcc cttgcggctg
     6901 gtcattgaga attacccgga gggacaggtc gagcaactcg aagccgtcaa caacccggaa
     6961 gacccttcag ccgggacgcg tcaggtgccc ttctcacgca ccctctatat cgatcaggaa
     7021 gatttccgag aagatccgcc aaagcagttc tttcgccttt ctccgggacg cgaggtgcgc
     7081 ctgcggtacg cctacatcat cacctgtgtc ggcgtggtga aggacccagc cacgggtgag
     7141 gttacggagt tgcgctgcac ctatgaccct gaaaccaaga gcggcggcgc gcaggcgcaa
     7201 cggaaggtga aggccacgat ccattgggta tcggcctcac acgccgtcga tgcggaagtc
     7261 cggctatacg agagcctcat gctcatggat ccaggcaaga ttcccgctga ccaggactgg
     7321 acccagcacc tgaatcccca ttcgctggag cgactgccga cttgcaaggt agagcccagc
     7381 ctggctgcga tcgcccccgg aaccagagtg caatttgaac gcgtgggata cttttgtgcc
     7441 gaccccgatt cgtcgcccga caagctggtg ttcaatcgga ccgtcacact caaagacacc
     7501 tgggccaaga tcgagaatgc tcagaccccg cgctgaacgg gaccggcacc gttcatcctc
     7561 ccatttcacc gtcccacaca aagtacgggg actccagcct tcggcaccct tgaaccgtgt
     7621 catgtcgatg aacgagaatc gccactcgca tgatcgagaa tcactccacg acaaaggagc
     7681 ctcaacgatg gtgacgggac cttgcagtga agagcatctt gaagcacagg gctcgtttcg
     7741 cgaacgcctc tcccggcttg aagctgcgat ccccggtgcg gcagccgaag cacaacccct
     7801 cctccggcac atctaccgcg ccacacgtcg tctgcacgac caactgcagc gcattgatcc
     7861 tacgcccctc ttgccgcggt ctgcggtgag cgaggtacgc tgttttgatt gcggaaccgc
     7921 caggatggag tcatttcaca catccacaca gggcggctac cacctctgtc cgacctgctt
     7981 ccagcaacgt ctccgcacgg gacgcgcgag gaccgcgcac tgagcctcac cttccctgtc
     8041 ccccaagaaa cgtgcatcgt tccgttcgag cggcacggtg gaccactccc tcccgcgccg
     8101 ctagccccaa gcccccgcca caccgtatac tgcaagcttt caacgtgatg aaagaggtac
     8161 cctgggtatg gccccgaaca ttctgttgag ttgcgaatcg atcagcaaaa gctatggggt
     8221 ccgagccctg tttacagatc tctccctggc actctccgac ggcgatcatg tgggattgat
     8281 cggaccgaac gggtccggca aatcgacctt gctcaaaatc ctcgtggggc tcgaatcccc
     8341 ggatgaaggc acgcgcaccc tgcggcgtca cacacgtgtc ggctatgttc cgcaggaacc
     8401 cctgtttcct ccacatcaca gcattgaaca agtgctgcaa ggcacgttga cggaagacgg
     8461 ccttgatcct cacgaacagg gtggccggat cgccagggcc ctcagcatcg gtacctttcc
     8521 cgacccggaa caagccgttt ccacactctc cggaggctgg cgtaagcgat tggccatcac
     8581 ccaggccttg ctactcgagc cggatgtgct gctcatggac gagccgacga accatttgga
     8641 cttggaaggc attatctggc tggagcaact gctgaagaat cgcgccaaag cctttctcgt
     8701 gatcagccac gatcgccggt tcctcgaatc ggtgacatct cgcatggtgg aactcaaccg
     8761 ctgttatccc gaaggccggt ttgaatcccg cggctgctat agtgatttcc tcgaacagcg
     8821 agatgccgcc cttcaagcac aagccgacta ccaggcctca ttagccaatc gcgtccgccg
     8881 tgaagtggag tggctgcggc gcggccccaa ggcccgcacg accaaagcca agggccggat
     8941 ccaatcagca ggcaaactca tcgacgaatt gaaccaggcc gaatcccgtt cggcacagtc
     9001 gaccatcggc atcgacttct ccgcctcggg ccgaaaatcc aaacaactcg tggtcgcgga
     9061 gcaggccagc aagagcttca acgggacgcc cgtcatcacc aatctcgacc tgctcctcgg
     9121 gcccggccaa cggctgggac tccttggtcc gaacggcagc ggtaaatcca ccctgctgcg
     9181 gttactggcc gggacgctgg agcccgattc cggcaccatc acgcgagccg acgcgctgcg
     9241 tatcgtctcc tttgagcaac atcgcgagtc cctggaccaa tcgacgagct tgcgacgcgc
     9301 actggccccg tcgggcgatg ccgtcgtcta tcaggatcgc tcgattcacc tggcttcatg
     9361 ggcgaaacgc tttctcttcc gtcccgaaca actggatctc ccggtctcga aactgtccgg
     9421 cggcgaacag gcacggctcc tcatcgcccg actcatgctc cagcctgcgg acctgctcat
     9481 cctggacgag ccgaccaatg acttggatat tccaaccctc gaagtgctgg aggatagcct
     9541 gctggaattc cccggtgcgc tcgtgctggt gacccatgat cggtggctgc tggatcgcgt
     9601 ctcgaccatc ctgctggcac tggatggcac gggacgcgtg gactggtttg ccgactatgc
     9661 ccagtgggaa agtgcgcagg agcgggccgg tggatccgcc ccggagtcga agagtgacgg
     9721 gcgtgctgcg gtccaggacg agactgcctc ggcaactccc gctcgcaaac ccaaaaagat
     9781 gagttatcgc gaacagaagg aatggggcac gatcgaagag agtatcctca aagccgagga
     9841 agaggtcaca gcctgccacg ccgcaatgca ggatccggct gtgctgtctg atgccgcagc
     9901 gctccaggca cgaagccagg cattggtggc ggcacaagct gaggtggagc gactctatgc
     9961 ccgttgggcc gaactggatg aaaaacgtgc acagactgtg cagagttcgt gagggaacga
    10021 ctgcccgtcc aaagttgtgg cgctgacagc ctcacatctc gacctgagtt gcgtacgttc
    10081 tcagaggatc gtctgaggag ggaaattggc tgggggacta ggaatcgaac ctaggtagcc
    10141 agctccaaag gctggcgtcc taccgctaga cgatccccca gcgcggtacg gaaccgagac
    10201 gtcgagctga agagcgaggc gtggaaaaga ttttggctgg gggactagga atcgaaccta
    10261 ggtagtcaga tccagagact gacgtcctac cgctagacga tcccccaacc ggtccacaca
    10321 gtaatataag cttctctact gcgtcaagat cgacgctgat tcttgaccgg ccgattctca
    10381 ggatctggag gcccgctcga tcccctggcg aagtcgaaac aaggtgcggt cccggccgag
    10441 cacgtccatc acctcgaaca gaccggggct cgccgtccgt cccgtcaagg ccacccgcac
    10501 cggctgggcc agttgcccca tttttatccc ctcttcctcg accagttgct taaatgactc
    10561 ttcccactcc tgcttggaaa acgtgggaaa ggcttcgaat cggctcagca gtttggctaa
    10621 caccggtgct tgagtggccg tgaggaattt cttggccgcc tcttcgtcga acgcggccgc
    10681 ctgaccgaag taaggtgtca cccagtggac catctcaacc aatgtcttcg ttcgttcctt
    10741 caccaagacg accagctgcg cgagccattc ggtcgacacg gcacgaacct cttctttgag
    10801 accggcctgc tcaaggagcg gcacgagcgc ctgagcaatc tcagccggag ggctggtctt
    10861 gaggtattca gcattgagcc acaacaactt gtccggatta aacacagccg ccgaggtctg
    10921 cacattcttc caggagaact tctcgatcag ttctgcgcgc gagaacagtt cttgatcgcc
    10981 atgcgaccag cctaagcgaa ccaggtaatt gaccatcgcg tcggggagat accccatgtc
    11041 cttgtaggcc atgatcgagg tggcgccgtg ccgtttcgac aaccttgcct tgtccggtcc
    11101 aagaatcatc ggtaaatgcc cgaatcgcgg caacggaaat cccagcgcct gaaaaatggg
    11161 aatctggcgc ggcgtattcg tgagatggtc atcgccccgc accacatggg taatccccat
    11221 caaggcatcg tccacaacca cggaaaaatt ataggtagga tacccattgg atctgagaat
    11281 gatgacatcg tccagaatct ggttgtcaaa caccacaggg cccttgatca aatcatcgac
    11341 aactgtctgc ccgtcctgtg gagccctaaa gcggagagcc gcttcccctg tcggttttgt
    11401 caggccgagg ttccggcacc gaccgtcata cttgggcgat ccgcctttgg tttcggcctc
    11461 ttttcgacgc gcctccagct cttccgaggt gcagacacac caataggcat ggccttgctc
    11521 gaataatcgc atcgcgtgtt cacgatagag atccattcgc tcggtctgcc gaaagggtcc
    11581 ttcgtcccaa tccagtttga cccagcgcat cccgtccaaa atggcctgaa tggagtcatc
    11641 ggtggaacgg ctctgatcgg tgtcttcaat gcgaaggaca aagaccccct tctgctgccg
    11701 cgcaaatagc cagttgaaca aggccgtccg taccccgccg atatgcagaa aaccggtcgg
    11761 actgggcgca aatcgaaccc tgacctgact catcgcatca ccgtagttcg tgtggaaata
    11821 ataaacgggg ggctatctat aacatggcgt tggaatgatg tacagaactg gcctgttcca
    11881 ttctgaatcc gtttttgtta agaacagacc atggccgaac caacacagat tgccgaaatc
    11941 gtctcgctca cgaccctgac tccgcacacg acggaactcg tgctccgccc gatcgagcac
    12001 ccgctgtcct tcaagcccgg gcagtggatc tcgctgcaat tgccggtcgg agaccatccg
    12061 ccattgaatc gtgcctactc gttggcggag ccgccgtcta ccgccggcca cctcacactg
    12121 ctcttcgacc acgttccagg cgggaaagga tccggctatc tcgcgtcgct caagccgggg
    12181 gatcgcattc ccctgtcagg cccctatggc cactttgtgc tgcccgactc ggaacctcag
    12241 cacttgctgt tgatcggacg ctattctggg ctcgtcccac tgcgctgtat gctccgcgca
    12301 ctggcgggcc agggcttgct accttcaagc acactgatcg cccaggcgcc caccactgcc
    12361 gaacagctct accatgagga attcatggcc ctggcggcaa gccaatctaa tttccggtat
    12421 ctcccgtttg tcagcgaagg ccccgagagc cctaccgaga ctgtggatgc gatcaaacaa
    12481 atcgcgggaa acggtcggca ggtgaccccc atgattgccg gggtcaaagc atttgcacgc
    12541 ccgttacggg cgcttctgat ggaactgggg ttcgatcggc gggaagtgaa gctggaaacg
    12601 tacgactgat acgagcgtta tacgacctgc actccatctc tttgccttca gtgcccttct
    12661 ttcgccagat tcttgttgat ggcaggaatt tctaccacga cttccaccgt tccattcggc
    12721 acacattggc agccgagtcg ggactgcaac gtcgtgacgg gcgcttcgtc caactggtcg
    12781 aactcgtcat ccgtcccctc attgcacgtc tccaagcctt tcttcaccat gacatggcag
    12841 gtggagcagg cgcagacgcc accgcacaca tgttccaggt cgatcccatt gcccatcgca
    12901 atgtcgagaa tacttcccgg caggcccgta ggaccgtacg gaatcttgtc cggttgtacc
    12961 tcaaccttct tttccgtttt gtcgggaaaa atgaaggtca cgatataaga tttttgcggc
    13021 agctccacgt ctgccttttc aatgtaggga tttgtcccac ccatgttgct tagccccttt
    13081 tgtcatgcat gtggtgcaat gtatcgtgct gcttccgtat cactcgtcgc aagcctccag
    13141 agagcgacat tcttacttct tccgcccctc cgctcgcttg acaggcccaa agaccacatg
    13201 ataccttttg tacgactgat ttgatcggag atcattatag tctccgactt gaaggatcgg
    13261 caataggccc atgctgaaat tatcgaaaaa agctgactac gggttgatgg cactccagca
    13321 catcgcctcc aaccaatacg gcgatgtcgc ccaagctcgc gtagtaaaca caaaggaaat
    13381 cgccgaggaa tatcacattc cggtcgaatt acttgccaaa gttctccaga ccctctccaa
    13441 gagcggaatc attgagagtc ataacggccc caagggcggc tatctgctcg gcaagaatcc
    13501 gcgtgagatc acgattgccc acgtactcga aagcctggaa ggtccactcg gtatcatgga
    13561 ctgttcacac gaaaaagacg gcgatgcctg catgcagcgc gagcactgta acattcggac
    13621 cccgttgctg aaaatccaga gcagtattta tcagcttctc aacaatatga ccttgcaaga
    13681 tatgatgggg ggcaccccac tcatcaccat tcaatccgtc gcgacggaac aacaaggagt
    13741 cgagcgatga agttcccgat ctacctggat aaccattcga ccaccccgat ggatccacgg
    13801 gtgctggaat cgatgctgcc ctattttacg gaaaaattcg ggaacgccgc cagtcgcaac
    13861 cacgccttcg gatgggatgc cgaagaaggt gtcgaagccg cgcgaaaaca gatcgccaag
    13921 ctcatccatg cggatgccaa ggaaattgtc ttcaccagcg gcgccaccga atcgaacaat
    13981 ctcgcgctca aaggcgtcgt cgagatgtat cacgaaaagg gcgaccacat tattacgtcg
    14041 tccaccgaac atcgcgcggt gttggacacg gccaaaaccc ttgaagccaa acgcggggtc
    14101 aaggtcacct atctgccggt cgataagttc ggcatggtga atccggaaga cgtgcggaac
    14161 gccatcaccg acaagaccat cttgatctcc gtcatgttcg ccaataacga gatcggcacc
    14221 atcaacccgg tcaaagcaat cgggaagatc gcgaaggaaa agggcattct atttcattgc
    14281 gacgcgactc agggcgtcgg aaaagtcccg atcgatgttc aggacatggg tatcgacctg
    14341 atgtcgttca gcgcccataa gatctatggc cccaagggcg ttggcgccct ctatgtcagg
    14401 aagaaaaatc ctcgtgtccg cattgcagca caaatggatg ggggcggcca tgaacggggc
    14461 atgcgatccg gcactcttcc ggtacccttg attgtggggt tcggaaaagc ctgcgaactg
    14521 tgcgagcagg aaatgtccgc agatggcgcg cgactctccg tcatgcgtga ccggctccat
    14581 gcgaccatta cgaaggcgct ggaagaggtc tacttgaacg gtcatccgac cgaacgcctc
    14641 ccgcacaatc tgaatatttc gtttgcctac gtcgaaggcg agtccctgct gatgggctgc
    14701 aaggaaattg ctctctcgtc cggctcggcc tgcacgtcag caaccctgga gccctcttat
    14761 gtgttgcgtg cattgggtgt gggagcggaa ctcgcgcatt cctccatcag attcggcctg
    14821 ggacggttca cgctcgacga agaagtggac tatgccggaa agagaattat cgaagtcgtg
    14881 accaagctcc gggaaatgtc accgctgtac gaaatggcaa aggaaggcat tgacctgaaa
    14941 tccgtgtcat gggcagcaca ttaacctgaa acttcactac tgtgagcgat acattcggag
    15001 gacaccatgg cctatagcga aaaagtcgtc gaccacttca acaacccccg taacgtgggg
    15061 agtttcaaaa aagaagaaga tggagtcggc accggtatcg tcggcgcccc ggaatgcggc
    15121 gatgtcatga aactccagat caaagttgaa aacgacacga tcgtggacgc caagttcaag
    15181 accttcggtt gcggctccgc cattgccagc tccagtctgg ccaccgaatg gctcaagggg
    15241 aaaacggtgg aggaagccgg caagatcaag aacaccgata tcgtgcagga gttgaatctg
    15301 ccccccgtca agattcactg ctccgtgctg gctgaagatg ccatcaaagc agccctcgca
    15361 gactatcaaa agaaagccga ctcgaagtaa cgacaggaag gagcgcacta tggacgccac
    15421 cacgaatacc gatgctcaag ccccggtcat cactctgagc gacgcagccc tgaaagaggt
    15481 caaacgtctc atcaatgtcc aaggcatcga agaaggcggc ctccgcttag gcgtcaaggg
    15541 aggcggttgc tccggcctga gctacaccat caatttcgac gaaaagatcg gccagtacga
    15601 tcaagtgtac gaattcgacg gcgtcaaggt cattgtggat gccaagagcg ccatttacct
    15661 gcagggcacc cagcttgatt ttcagaagga cctgatgggc ggaaacttca aattcgtgaa
    15721 ccccaacgcc aacaagacgt gcggctgcgg ggaatccttt tcggcctgag cgagcgacag
    15781 cccatgagcg gacatattca taacaccgat gatcgacgcg agctgcagat ggctcgcagc
    15841 atgtgctggc attgccagtc cgaaatggct ggagagtact tttgcgaccg ttgcgtgaaa
    15901 gtccaaccgg tctccaagga tctggactat ttcacctgct ttgggctgcc acggcgcctc
    15961 gtgattgatc cgcagacact ggaaaccaag ttctacgagt tgagccgggc attccatcct
    16021 gacttctacc agaataaaag cgatgcggag cagaccatca gcctcggcaa ctcggccatg
    16081 ctgaacaccg cctatcgcac cctacgagac ccgattcaac gagccgaata cctattggac
    16141 ctcgaagcgg gctccgtgaa agatatccgg acgacaccgc cggccgatct cttcgaagaa
    16201 attctcgagt tgcaggatac cctcgatgag ttccgcgcca gtgatcgcgc ctccgaaagt
    16261 gcgtctaccc tccgcacgaa actccacgct gatcgcgcca cacttgagca acggcaacgg
    16321 gatatggaag cccaactcca acagcttttc agccggtggg atgcactgca ggatcgtggc
    16381 gaggccacgg agcaggcacg cgctgagcgc ggccgcattc tcaaggaaat gcgggacatc
    16441 ctgtccaacc gcacctacgt gaacaacatc gtcaacgatc tcgtcgcaac gatcgcctaa
    16501 tcattatggc acgcattgtc ggcatcgacc tcggcacgac caattccctt gtcgcttaca
    16561 tggatcatgg cacgccacgc gtcatctcgg cacgccacga acgcgcgatg gtcccctctg
    16621 tcgtcgcgct gaccgacaac ggactcattg tcggcgaccc ggccaaagaa catctcacca
    16681 gaaatcccga gcgcacggtc tactccgtca aacggttcat gggcaagagc ctggccgacg
    16741 tccaaaacga actggcctac tttccctaca cgctcacaga aaaaggtggc gtcatccgga
    16801 tcaagctggg agagaaatcc tactccccac cccagatctc tgccatgatc ctgaaagaac
    16861 tcaagctccg tgctgaagcc tttctgggag aaagcatcac caaagccgtc attaccgtgc
    16921 ctgcctattt taacgatagc cagcggcagg cgaccaaaga cgcaggcatg attgccggac
    16981 ttgaagtctt gcgcatcatc aatgaaccaa ccgctgcctc actggcctac gggttgcaag
    17041 aaaagacgca gggcaccatc gccgtctacg acctgggcgg tggaacgttc gatatctcga
    17101 tcctgaaatt aaaaaacggg atctttgaag tgctcgccac aaatggcgat acgcatctcg
    17161 gcggcgacga cttcgaccag ttgattgccg acctgttcct cgctgacatc cgcacgcagt
    17221 atggactcga tctcagcacc tattccgacc atatgcaggc agtccgcctg gaagccgagc
    17281 gcgcaaagat tcgcctttca gaagaactca agaccgccgt gagtctcgac ctcccggaaa
    17341 gtaagggccg gtttacacgc gaactcacac gcgatcaact tgaagggctc tgtaccgcat
    17401 tggttgagcg tacgctgggt ccctgccgcc tggccttgaa ggattcgggg ttaactccgg
    17461 gcgacatcga tgaagtcgtc ctcgtcggcg gctcgacacg catgccgctg gttcgccaac
    17521 gagtgcaggc cctcttcggg aaacgaccgc actgcgagct gaatccggac gaagtggttg
    17581 cgctcggcgc tgcggtgcag gcggatattt tgagcggcgg cacaaccgat atgttgctgt
    17641 tggatgtgac ccccctttca ttgggtatcg agacaatggg cggggtcatg agcagtctga
    17701 tcagacgaaa caccaccatc cccgccagcg ccaaggaaat gttcaccacc tatgtggacg
    17761 gccaaaccgg ggtggacatc catattctcc agggcgaacg agagctggca aaggataacc
    17821 gcagcctggc ccgtttccgt ctcaaggtgc cgccattgcc cgcgggagtt ccacgcattg
    17881 aagtgacctt cttgatcgat gccaatggca tcctgaatgt catggccaaa gacatgcgga
    17941 ccggagagac ccaatcgatc gaggtcaaac cgtcatacgg attatcggat caagaagtcg
    18001 aacggatgat cgaagattcg ttcaagttcg ccgcagacga tgtcaacgcg cgaaaattga
    18061 tcgaagcccg actcgatgcg ggagccctga tcaccacgac cgaaaaatcg ttggccgatg
    18121 gtgggcatct cgtcgccaca ggggacgtcg aggccattcg caccgccctc tcggccctgg
    18181 cgaccgccaa ggacggaagc gatccccgtg ccatccgcgc acgcatggct gacattgagc
    18241 aggcagccaa aggcctcacc gtggccatgc tggacgattc cctgaagcaa gggctccaag
    18301 gcaaaaaagt ctctgacgtg acgcaatcct gaactcgagg agttgcaatg gatttgaagt
    18361 ggagtgacac tgaagaactc gccatccgtt tggtggaggc ccacccgacc accgaccccc
    18421 tcaccgttcg cttcaccgac atgcatacat ggatcgtcgc cctcccagaa tttaaggacg
    18481 acccgaagaa gtcgaacgaa aaaaccctcg aatccattca aatggcctgg cacgaagaat
    18541 atcaggactc ccagtcctga ccggccaacc gaacgtcctt caccacacaa cacatctcac
    18601 agccaggccg gcgtcggcgt catgaccatc gtagtgtggt gctgtgcccg gatgggcgga
    18661 gaagatttgg gcaggaaact tgcccggcgt cgggctacgg ctcgagagaa actggtgggg
    18721 cactgtcctg caattgg
//
LOCUS       DAORQN010000020        17829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_209_length_17829_cov_23.588950, whole genome shotgun sequence.
ACCESSION   DAORQN010000020 DAORQN010000000
VERSION     DAORQN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209_length_17829_cov_23.588950"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /gene="arfB"
                     /locus_tag="PK614_02705"
     CDS             complement(<1..226)
                     /gene="arfB"
                     /locus_tag="PK614_02705"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRB81149.1"
                     /translation="MLSISTHVTIPDEEIELTAVRAQGAGGQHVNKVSSAIHLRFNIL
                     ASSLPPFYKERLLALRDHRISRDGTIIIKAQ"
     gene            426..2198
                     /locus_tag="PK614_02710"
     CDS             426..2198
                     /locus_tag="PK614_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81150.1"
                     /translation="MEPALNNAPSKAGLVSDRSRSTQRGLPLVLLLCFVIATALGLYA
                     LSFLRTVLVSERGRELAMNAAAVADALDRVMFERFGDIQLFANDGILRDGTSAEKTAR
                     LLAYKQLYQYYSWLGVADENGQLTAATDALPAQGTQGLNPESFELVRRTGKVHFEDMR
                     PSPASQRKMAIGFTAPIYGPKGEFRGVVVTRVPFENLQSIFEQEGRLRYGELAYDWLL
                     LDREGTVLSEKHRPSNLNGNPVKIDLPSQAQAVIHRDHPGFVQETHRRGGNAVVTGYA
                     WTRGYANFPGFDWLVLFRLDRDQVYAPIDRLIWMVGGIGLLVILPLTGYGIWVSWELG
                     REKNDLVEARQKLEESVAELGRSNADLQQFAYVASHDLQEPLRMVSSYTQLIARRYKG
                     KLDADADEFIAFAVDGANRMQRLIQDLLAYSRVNTTGRQFEPTAMETVLKAALSNLTN
                     AVKESQAVITHDPLPAVMGDDKQLAQLFQNLLSNAIKFSGAQPPRIHISAKQADGEWL
                     FSVRDHGIGLDPQYADRIFVIFQRLHTRAEYPGTGIGLAICKKIVERHGGRIWVESQL
                     GKGTTFYFTMRDEEPGTSAHTPNR"
     gene            2222..2683
                     /locus_tag="PK614_02715"
     CDS             2222..2683
                     /locus_tag="PK614_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB81151.1"
                     /translation="MVPEMVKPIEILLVEDNPGDVRLTMEALKEAKVVNHLTVLKDGE
                     EALTYLRRQGPYAHATRPHLILLDLNLPRKDGREVLAQIKAEEPLKRIPVVVLTTSED
                     EQDVLKSYNLHANCYITKPVDLDQFIRVVRSIEDFWLGIVVLPVPPQNGTR"
     gene            2680..5676
                     /locus_tag="PK614_02720"
     CDS             2680..5676
                     /locus_tag="PK614_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB81152.1"
                     /translation="MSTGPAHILLVEDNAGDARLLRELLTETGAGRLTLTHVDRLDAA
                     IRCLTQAAFDIILLDLSLPDSQGAETLVRMHAAAQGIPIVLVTGREDEELGLQLIQAG
                     AQDYLVKGQTTAPLLARALKYAVERKRLEEELRQKTRFLQSVLDNMAEGVVMADERGT
                     FQVWNQAAERIIGSGPADIGIGEWSKHYALFLPDRVTPFPTDDLPLARAIRGESVTDV
                     LIFFHRPHWPGEAWLSVTARPLLDDTGHLSGGVAVFRDITAAKRTDEALRDSQERYRL
                     LVTKANDIIYRTDASGRFTFVNPVAMRIMKYTEPELLGRRFIELIHPDHQPAAERFYG
                     RQFVRKQPSTYYEFLALAKDGSEVWIGQNVQVLLEEGQVVGFQAVARDITDRKRAEEA
                     RLESEERLRSIVQSTGDAIILMDTRGQVAFWNSGAEKIFGYTAEEMLGQPVTRIIPQR
                     FREAHQRGVERVAAAGRLTLQADMFELIGLRKDGTEFPLEFSLAAWTAKSKLFITGII
                     RDISERRQAETALRDSEERFRAIMNNSPALIFIKDLAGRYLQANRQFEIISHLSHEDL
                     VGKTDEEVFPSEQATVFRDNDRKVLETGEPMRFEESALHEDGLHTSIVVKFPLRNAQN
                     RCYALCGIATDIADRKRAEEARQQLAKDRLLLLESSGEGIYGIDREGRCTFINSAAAR
                     MLGYLPDELLGQDMHERIHYSFTGGTAYPRAACRIQETLERGKGCKVDDEVYWRKDGT
                     DFPVEYSSFPIIEQEQIIGAVVVFLDTTARKRAEQQLTLSHDQLRKLTARLESVREEE
                     RILIAREIHDELGQALTGVKLDLCLLRDQISEVSPAMMKRLESISTLVDGTIQSVQRI
                     ATELRPVVLDQLGLIPAIEWQAHEFQARTGIQCTLDVYLRAITLSHAGSTAMFRIFQE
                     ILTNVARHAQASIVHITLQEQAGSLVLDVRDNGRGVTDAELADPHSLGLVGMRERALL
                     LGGETIVTGSPGTGTAVRVRIPLGQP"
     gene            5755..6399
                     /locus_tag="PK614_02725"
     CDS             5755..6399
                     /locus_tag="PK614_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81153.1"
                     /translation="MTRILVVDDHAVVRQGVKQILSEQFHGAVLGDAQNAEEMIAQVG
                     KHSWDIVILDVGMPGKSGLDALKELKQARPKLPVLVLSGYPEDQLALRMLKAGAAGYL
                     SKDSAPNELVQALRKILGGGKFVSAAVAELLALNLESDWEKPLHEQLSDREYQVMCLI
                     AGGKSLKDIADDLCVGVSTINTYRARILEKMQLKNNTELTHYAIEHRLVNRLIR"
     gene            6672..6959
                     /locus_tag="PK614_02730"
     CDS             6672..6959
                     /locus_tag="PK614_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81154.1"
                     /translation="MTELYETEALIIRELEQQGPCTLDALAYRLPACTWNQIFIAVDA
                     LSREGTISLQPRAGCQYLVSLASVHAHTLHHFDPDRRVAETRQHTGRSYGR"
     gene            6975..7406
                     /locus_tag="PK614_02735"
     CDS             6975..7406
                     /locus_tag="PK614_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81155.1"
                     /translation="MSATMNSTKQTGRSRVMIVDPEWQFGLKLADCLATNGYHAVLVR
                     DLQSMLTELGEIQPEAILLSSDSCDEERGTAELDTLRALNVLCPQAPILTVVEPMRDA
                     LGTLSPPIDARPRHQAPLVHNRVEELLRANLGIPCVRASLT"
     gene            7462..7773
                     /locus_tag="PK614_02740"
     CDS             7462..7773
                     /locus_tag="PK614_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB81156.1"
                     /translation="MAQRRLRSIEDSVRIPSRSTTEMETSARQRSGPESPPQQADNEA
                     TTEEIRTRIEKLAYELYQQRGRQDGHDRQDWLEAERLTLTEPTRARQDRSDRLPTSVQ
                     A"
     gene            7943..8233
                     /locus_tag="PK614_02745"
     CDS             7943..8233
                     /locus_tag="PK614_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81157.1"
                     /translation="MRAREGLQLLFSGQHEYVFSYGPSLSYLKVVLSTVALNLFSTHG
                     LEPVRLATHVAEWALLMGQVCGTVRPSGDLVLSSCYEYCRRQAVTHSSVSPR"
     gene            8255..9004
                     /locus_tag="PK614_02750"
     CDS             8255..9004
                     /locus_tag="PK614_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81158.1"
                     /translation="MIHERGSGSALITIAIVSQNQLLRLGLQAVIAPHAHIRLIGEAT
                     NMLQAVSIVTQEQPQLLIIELEPGMDILGLVRKVKASLSTIRIIALHGIEDSRAGLEA
                     LSSGIDGMVLTTQPGVVLLAIIEYLCRRPANVARCHPCDASALETTGSAGGFEDRIPV
                     LPNWPEDLTKREHDIITLIGDGLSNKDIACRLSICSTTVRHHLTSIFGKLDVRSRQQL
                     LIRAHKYGLVKLHANGTAAHAHLVAGSEQSA"
     gene            complement(9161..9811)
                     /locus_tag="PK614_02755"
     CDS             complement(9161..9811)
                     /locus_tag="PK614_02755"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB81159.1"
                     /translation="MNHRFVSLSHPCPSTSRSVLGLIVCLLAVMLVAVSGHATSSWAG
                     QTPTEIVERTMKDMLYILTELKDASRSAQRQWEIEQVVRRAFNYEEMAERSLGETGIK
                     SNAVERRQFVRLFVQVLRDDLADHLRDYSAAQVVYLAEGNDADGAQVMIAPAGQEVDT
                     RIEFQVVRRSGAWLVNDISIDGSSIMAKYQAQFTRILREGSFSDLMEYLKQKAVIA"
     gene            10014..11264
                     /locus_tag="PK614_02760"
     CDS             10014..11264
                     /locus_tag="PK614_02760"
                     /inference="COORDINATES: protein motif:HMM:NF023301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HRB81160.1"
                     /translation="MSHRRMPLLLLSLSIPALFSGCETTDKMLGAAERAVGSSTGRTV
                     LDIVGGKDPAEIARRRVENYGRDPQALLRDLRSIQRDFQALMAALTGEVGKKWGTKEV
                     KLPEQKKYVKYTQNYRSRAIVDFDAGNILVETLDEKDPRASLKNAVVTTLLTPNDPRS
                     VDLFTDKEITLTSDKEPYLLGLVLDQAGKPVRTPAEAEQFADTLLSKSTTTRKVEQEE
                     GPKTAHMVNISMVTNFSHKQAEKYRAVVGQFAERYQISPSLVFAIIRTESNFNPFAVS
                     SAPAYGLMQLVPTSGGRDAYRKAKGEDKAPSRDYLFDPDNNIELGTAYLNVLTYAQLD
                     DVTDLVSREYCVISAYNTGAGNVFKTFSKDQRNALQQINGLQPAALYDRLRTGLPYQE
                     TRDYLAKVVGFRKQFVSLGDTAAK"
     gene            complement(12969..14063)
                     /locus_tag="PK614_02765"
     CDS             complement(12969..14063)
                     /locus_tag="PK614_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB81161.1"
                     /translation="MQGPVLSIIVPTFNERDNIPRLVATLDACLVGVEWELIVVDDDS
                     PDQTAAVVRAQACANHRVRCLHRIGRRGLSSACIEGMLSSSAPVVAVMDGDLQHDERL
                     LPDMLKVLLQGGYDIVVGSRYMAGGSIGEWDGMRALTSRWATRLSRPLVPAGLTDPMS
                     GFFAMRREVFDRLVRRTSGMGFKLLLDVLASSPHPLRFIELPYEFRSRQAGESKLDSQ
                     VAWDYVMLLLDKSIGRFVPVRLVTFCLVGGSGVLIHFSLLWVLYRWLAVPFAWGQGIA
                     TLLAMTSNFILNNIITYRDQRLRGWQWLRGWLSFVLVCSLGAVGNVGVASYLFTQQFH
                     WGLSALAGILVGTVWNYAVTSSYTWKVKAR"
     gene            complement(14200..14925)
                     /locus_tag="PK614_02770"
     CDS             complement(14200..14925)
                     /locus_tag="PK614_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRB81162.1"
                     /translation="MDKALYPYLLFWSIGLLCFLAEWRFPARPIAYRSVFWRDLIALG
                     LYNVSFLAVVQATDRIPIPNYMPAALYNLPTVVKLALFYIVEDFGLYWVHRLMHTKPV
                     WRIHRWHHSPTSLYWLSGIRATIPHIALFNLTYIIALPLLHEASSWAFQVIMVEHIVR
                     NNWMHLNVTWRSSWLEWVFVTPRYHQIHHSTDPAHQRTNLGALLTIWDRLFGTYYNPD
                     DVRGELSFGLAERVPPVRLVIGL"
     gene            15456..15947
                     /locus_tag="PK614_02775"
     CDS             15456..15947
                     /locus_tag="PK614_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81163.1"
                     /translation="MKRHIATYSAIVLACSWALLGPAPTQAAADNIEPVTWSNSQKSS
                     AWAEELLGQVVTYQTLAEKSLIPGNFEAYVEQMRKVRELYRTGNRRATYDGVNQLMVM
                     LEARVGGIDAHSADALWDFCYRVTPDEYHARDRHIRAKGADEVKKHEDFMRDMEERAS
                     MAF"
     gene            16063..17766
                     /locus_tag="PK614_02780"
     CDS             16063..17766
                     /locus_tag="PK614_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRB81164.1"
                     /translation="MLRSTLTRTVLLVCVILLESTGATLVLSPVWAESERAGYGPASP
                     GLSSSPVRLRFEPDDAVSSPLGSSFDLQHVWMRADLRVRPEWRNGVCFGGGPPIAGAC
                     NSLDQFGSGTGAHSGRHAGDFFIQQWARIGLGYDPSPNLNFYVELQDSATWGGNGDPT
                     ERNQVGDASTHNCGIQLAGQCRLGIRAGYMLVRNIAGIDGFSAKIGRQYVVFGNQRLF
                     GHFDWANTGYSHDGIMLSYATAAFDTKLGWFRNSETDLGQASPGGSLTPNLLTCNPAA
                     NASATCNPALAQHATDAGSDVDLVVFYSQLRSIPRMIVEPYYVLYSNRLPERANPGQY
                     LPKSASQLRHMVGLRIEFRDGNWDFTHEAAYQFGRSADGFDFDNRRNLRINAWASGTW
                     LGYTWYTHHWKPRVAIGFDYASGDGDSNCVPPGGNLARSCGGNANTFENFFPTNFLHV
                     GYMLNGAWRNSVQPQVNVQARPTARDHIELWALRKYLASARDNWYRGSQGPLIFSASD
                     NRTTHIGDELDIAWSHMFADGTVSLSIIYGHFFSGSYIKHQLGTAADQDWGIVQLWTN
                     F"
BASE COUNT         4006 a         5404 c         4948 g         3471 t
ORIGIN      
        1 cctgcgcctt aataataatg gtgccgtcac gcgagattct atggtcccgt aaggccagca
       61 gtcgctcttt gtagaacggc ggaagggaag acgcgagaat attgaatcga agatggatcg
      121 cggaggaaac cttattgaca tgctgcccgc ctgcgccttg cgcccgcaca gcggtcaatt
      181 cgatctcctc gtccggaatg gtcacatgcg ttgaaataga caacatagcg gtgaggtcct
      241 gcgcccgaca gtgaagagaa cgtacttggg agacagggcg ggagtcaatc acgtgagcac
      301 accacacctt gttcgcggtc gagcattcac ccggtcacac gcccctatct gccggatgga
      361 actgtatccg tgaacagggt atgatactaa ggtcttcccc tttaaacccc tgacacaaga
      421 gtgcgatgga acctgcgctg aacaacgcgc cctccaaggc tggcttggta tcagacagaa
      481 gcagatcgac ccaaaggggc ctcccgctgg tcctgctgct ctgttttgtc atcgctactg
      541 cactgggact ctatgcgttg tcgttcctgc gaacggtgct ggtctcagaa cggggacgag
      601 aattggccat gaatgccgcc gcagtcgcgg atgcgctcga ccgcgtcatg ttcgaacggt
      661 tcggcgatat ccagctgttt gcgaacgacg gcatactccg agacggcacc tcggctgaga
      721 agaccgcccg gctcctggca tacaaacaac tctatcaata ttactcctgg ctgggggtcg
      781 ccgatgaaaa cggacaactc acggccgcca ccgacgcgct gcccgcgcag ggcacgcaag
      841 gcttgaaccc cgagtcgttc gagttggtgc gcaggacagg gaaggttcac ttcgaagaca
      901 tgcgaccctc cccggcatcg cagagaaaga tggcgatcgg attcacggca cctatctatg
      961 gtcccaaagg agagtttcgt ggggtagtgg tcacacgagt gccgtttgag aatcttcagt
     1021 cgattttcga gcaggaaggc agactgcgat acggagagtt ggcctatgac tggctgctgc
     1081 tcgaccgaga agggacggtc ctcagcgaga aacatcgccc ctcgaatctg aacggcaatc
     1141 cggtaaagat agatttgcct tcgcaagccc aagccgtcat tcatcgagat caccccggtt
     1201 tcgtgcaaga aacccatcgc cggggaggaa acgcagtcgt caccggctat gcctggacca
     1261 gagggtatgc caactttccc ggattcgact ggctggtctt gtttcgcttg gaccgcgatc
     1321 aggtctatgc cccgatcgat cgactgatct ggatggtggg agggattgga ttgctggtca
     1381 tcctccccct caccggctac ggcatctggg tctcgtggga actgggacgc gagaagaatg
     1441 acctggtcga ggcccggcaa aagttggagg agtccgtagc ggaattaggc cgctccaatg
     1501 ccgacctcca acaattcgcc tatgtggctt cgcacgactt gcaggagccc ctccgcatgg
     1561 tgagcagcta cacgcagctc attgcgaggc gctacaaggg aaaactcgat gcggatgcgg
     1621 atgaatttat cgcctttgcc gtggacggcg ccaaccgcat gcaacggctc atccaggacc
     1681 tcctggccta ttcgcgagtg aacacgacgg gccgacaatt cgaaccgacg gccatggaga
     1741 cggttctaaa ggctgcgctg agcaatctaa ccaacgccgt caaggagagc caggccgtca
     1801 tcacccatga cccgttgccg gcggtgatgg gcgatgacaa acaattggca cagttgttcc
     1861 agaatctgct gagcaatgcg atcaaattca gcggggcgca accgccgcgc atacacatct
     1921 ccgcgaagca agccgatggt gaatggttgt tttccgttcg cgatcatggc atcggactcg
     1981 acccacaata tgcggatcgg atttttgtca ttttccagcg gctccacaca agagcagaat
     2041 atccggggac cggtatcggc ctggccattt gcaagaagat cgtcgaacgc cacggcggac
     2101 gcatatgggt ggaatcccaa ctcggcaagg gaacgacatt ttatttcacc atgcgcgatg
     2161 aggaaccggg cacctcggcg cacaccccga atcgctgacg gaaggaagga gtaggagttg
     2221 tatggtacct gagatggtga agcccatcga gattctgctg gtcgaggata acccgggcga
     2281 tgttcggctg acgatggaag cactgaagga agccaaagtc gtgaaccatc tgaccgtgct
     2341 gaaggacggt gaggaagcct tgacctatct gcgccgacag ggtccctacg cccacgcgac
     2401 gcgaccccac ctgatcctgc tcgacttgaa tctgcctcga aaagacggtc gggaagtgct
     2461 ggctcaaatc aaggccgagg agccgctgaa acgcattccg gtggtcgtgc tgacgacctc
     2521 agaggacgag caggatgtcc tgaagagtta caaccttcac gccaactgct atatcacgaa
     2581 gccggtggat ctggatcagt tcattcgggt ggtgcgatcc atcgaagatt tttggctcgg
     2641 gatcgtcgtc ttgccggtcc caccacaaaa cggaaccagg tgagcaccgg tcccgcacat
     2701 atcctgctgg tcgaagacaa tgccggcgat gctcgtcttc tgagggaact cttgacggaa
     2761 accggcgccg gccggctcac cctcacgcat gttgaccggc tcgatgccgc catccgatgc
     2821 ctgactcagg ccgcgttcga catcattctc ctggatctct cgttgccgga cagccaaggg
     2881 gccgagaccc tagtcaggat gcacgcggcg gcgcagggga ttccaatcgt gctggtgacc
     2941 ggacgggaag atgaggaatt gggactacag ctcatccagg cgggagccca ggactatctc
     3001 gtgaaaggac agacgaccgc cccgctcctg gcacgcgccc tgaagtatgc agtcgagcgc
     3061 aagcggctgg aagaagaact gcggcagaaa acgcgctttc tccagtccgt gctggacaac
     3121 atggccgaag gcgtggtgat ggccgacgaa aggggaacgt tccaagtgtg gaaccaggcc
     3181 gctgaacgta tcatcggaag cggtcctgcc gacataggaa tcggagaatg gtccaagcat
     3241 tacgctctat ttttaccgga cagggtcaca ccattcccga ccgatgacct accgctggca
     3301 cgagcgatcc gtggagaatc cgtgaccgat gtcttgatct tttttcaccg gccgcattgg
     3361 ccgggcgaag cctggctcag cgtgacggcc aggccgttgc tggatgacac cgggcatctc
     3421 agcggagggg ttgccgtctt tcgagacatc accgctgcaa agcgaaccga tgaagcgctc
     3481 cgggacagcc aagaacggta tcgactgctg gtcactaagg ccaacgacat catctataga
     3541 acggatgcat cgggtcggtt cacctttgtg aacccggtcg ccatgcgcat catgaaatat
     3601 acggaaccgg aactgttggg gcgtcggttc atcgagttga ttcatcccga tcatcagccc
     3661 gcggccgaac gattttacgg acggcagttc gtccgtaaac aaccaagcac ctattacgaa
     3721 tttctcgccc ttgccaagga cgggagcgaa gtgtggatcg gccagaatgt gcaagttctt
     3781 ttggaggagg gccaggtagt cgggttccag gcggtggccc gcgacatcac ggaccgcaaa
     3841 cgggcggagg aggcacggct ggaaagtgaa gaacggctgc ggtcgattgt gcaatcaacc
     3901 ggcgacgcga ttattctgat ggatacccga gggcaggtgg ccttctggaa cagcggcgct
     3961 gaaaagatct ttggttatac ggccgaggag atgttggggc aaccggtaac ccgtattatt
     4021 ccacaacgat tccgcgaggc gcaccaacga ggcgtcgaac gggttgccgc cgccggacga
     4081 ctgaccttac aggcagacat gtttgaactg atcggtctga ggaaagacgg aactgagttt
     4141 ccgttggagt tcagcctggc ggcttggaca gccaagtcca aactcttcat taccggcatc
     4201 attcgcgata tcagcgagcg caggcaggca gagacggcac tgcgagatag cgaagaacga
     4261 tttcgagcga tcatgaacaa tagcccagcc ctgattttca tcaaagacct tgccggacgc
     4321 tatcttcagg cgaaccgtca gtttgaaatc atttcccatt tgtcccacga ggatctcgtc
     4381 ggcaaaaccg acgaggaagt cttcccttcg gaacaagcca ccgtatttcg ggacaacgat
     4441 cgcaaggtct tagagaccgg agaacccatg agattcgaag aatcggcgct ccatgaggac
     4501 ggtctccaca ccagcatcgt cgtcaagttt cccctccgca atgcccagaa tcgttgttac
     4561 gccctgtgcg gcatcgcgac cgatattgcg gatcgaaagc gagccgaaga agcccgacaa
     4621 cagctcgcca aggatcgttt gctgctgctg gagtccagcg gggaagggat ttatggcatc
     4681 gaccgtgaag gtcgctgcac attcatcaat tcagctgcag cgcgcatgct gggctatctc
     4741 ccggacgagc tcctcggcca agatatgcat gagcgcatcc attattcctt cacgggtggc
     4801 accgcatacc cccgtgcggc ctgccgtatt caagagacgc tcgagcgcgg aaaagggtgc
     4861 aaggtggatg acgaagtgta ctggcgcaag gacgggacgg attttccggt ggagtactct
     4921 tcgtttccga tcatcgaaca agagcagatc atcggagccg tggtggtgtt tctcgacacc
     4981 accgcgcgca aacgggccga acaacagctc accctctcgc acgatcaact cagaaaactg
     5041 accgcgcggc tcgaatcagt tcgggaagag gagcgaattc tgatcgcccg tgagatccac
     5101 gacgaattgg ggcaagcgtt gacaggcgtg aaacttgacc tctgcctgtt acgcgaccag
     5161 atctccgaag tctctcctgc gatgatgaaa aggctggagt cgatttccac gctggtcgat
     5221 ggcacgatcc agtcggttca acgcatcgcc acagaattac gccctgtcgt cctcgaccaa
     5281 ctcggcctca tccctgccat cgaatggcag gcccatgaat ttcaagcacg cacagggatt
     5341 caatgcacgc tcgacgtcta tctgcgcgct atcacgttgt ctcatgccgg ttcgacggcc
     5401 atgttcagaa tttttcagga aatcctgacc aatgtggctc gccatgccca ggcgtcgatc
     5461 gtccatatca cccttcaaga gcaggccggc agccttgtgc ttgacgtgcg cgacaacggg
     5521 cgcggcgtca ccgatgctga attggccgac ccgcattccc tgggcctggt gggaatgcgg
     5581 gagcgagccc tgcttctggg aggtgaaacc atcgtcacag ggagtcctgg gacaggcacg
     5641 gcggtgaggg tccggattcc tcttggtcag ccatagtcgg cccggcgccg gaacgcaaca
     5701 gggggcactg ctcgttcaac ctctcaggcg cgatagcgga aggaggaaca ggttgtgaca
     5761 agaattctcg ttgtggatga tcacgccgtc gtccgacagg gcgtcaaaca aattctcagc
     5821 gaacagttcc atggggccgt cctcggcgac gctcaaaacg cggaggagat gatcgcgcag
     5881 gtagggaagc acagctggga tattgtgatt ctcgatgtcg gcatgcccgg gaagagcgga
     5941 ctcgatgcgc tcaaagagct caaacaggcc cggccgaaac tccccgtcct cgtcctgagc
     6001 ggttatccgg aagaccagct ggcattacga atgttgaaag ccggcgcggc cggctacctg
     6061 tcgaaagaca gtgcgccgaa cgaattagtg caagctctcc ggaagatcct cggcggcgga
     6121 aaattcgtca gtgccgcagt ggcggaactc ctggccctga atctggagag tgattgggag
     6181 aaaccgctgc acgaacaatt gtctgatcgc gagtaccagg tcatgtgttt aatcgcgggg
     6241 gggaaaagct tgaaagacat cgcggatgat ttgtgcgtcg gtgtcagcac catcaatacc
     6301 tatcgggcgc ggatcctcga aaaaatgcaa ctgaagaaca acaccgagtt gacgcattac
     6361 gcgatagaac atcgtctcgt caaccggctc atcaggtgag atccttcctc tctgtcatca
     6421 ttgcctatcc gttcccgctc cacaagctcc cttcgtagca taaacgtcga cacgcgccgt
     6481 acagaaaacg gcgacgcgta aattgcgtaa aaggctatgc ttttcggaac ctgcttccgg
     6541 tacagatagc actgacgcgg cgatctggtt gcgagaccgt tcgcaccgtc attcgcaaga
     6601 gagggccggg tggcatggtt gcgctggtcc ctccttacaa ctcagacgat gaccggtcag
     6661 agagggatga catgaccgag ctctatgaga cagaggccct cattatccga gagctggaac
     6721 agcagggccc ctgcacattg gatgcactgg cctatagact ccccgcctgc acctggaacc
     6781 agatattcat cgcagtcgat gccctgtcgc gggaaggaac gatctccctc caaccgcgcg
     6841 ctgggtgcca atacctggtt tctctcgcgt ctgtccacgc acacaccttg caccacttcg
     6901 atcccgatag gcgtgtagcg gaaacccggc agcacaccgg ccgttcgtac gggagataag
     6961 agggagatcg acacatgagc gcgacaatga acagtaccaa acagaccgga agatcccgtg
     7021 tcatgatcgt cgatcctgaa tggcaattcg gactcaaact ggcggattgc ctcgctacca
     7081 atgggtacca tgcagttctc gttcgagacc ttcaatccat gctcaccgag ctcggggaga
     7141 tacagccgga ggccatcctg cttagttcgg actcttgcga tgaggaacgg gggaccgctg
     7201 aactcgatac tcttcgagcc ctcaacgtcc tctgcccaca agcgccgatc ttgacagtcg
     7261 tcgaaccgat gcgagacgcg ttgggtaccc tttctccgcc aatagacgct cgtccacgtc
     7321 atcaggctcc gctcgtgcac aatcgcgtcg aggagttgct gcgagcaaat ctcggtattc
     7381 catgcgtacg cgcatctctg acgtgatgcg ctcatcttgg gaagaacacc actcactcgg
     7441 actcaacagg gaggttcagt catggcacag cgacggttaa ggtcaatcga agacagcgtc
     7501 cggataccga gtcgatccac gacggagatg gagacctcgg cacgacaacg gtcgggtcct
     7561 gagagcccgc cgcagcaggc cgacaacgaa gcgacgaccg aggaaatcag gacgcgcatc
     7621 gagaagctgg cctatgagct ctatcagcaa cgagggcggc aggacgggca cgaccgtcaa
     7681 gactggctgg aagccgaacg attgacgctg acggaaccga cgcgcgcgcg ccaagacaga
     7741 tctgaccgcc tcccgacctc tgtccaggcg taacgcgtgg gacgatgaga agaccgcaca
     7801 cggaagatcc tgcacggaga acagtacgat gctctgccca accaccaact gcggccaact
     7861 aatggcatct gattatcgtg caggctatga cacacgcact ggtcttgaat gcctccagtg
     7921 tggacactgc gggcatcgtg gggtgagggc acgagaaggg ctgcagctgt tgttctccgg
     7981 gcaacatgaa tatgtcttca gctatggtcc gtccctctcg tacctcaagg tcgtgctgtc
     8041 gacggttgcg ctgaatctgt tcagcaccca tggcctggaa ccggtccgac tggcaaccca
     8101 cgtcgccgaa tgggcactcc tgatggggca ggtctgtggc acggttcgcc cttcaggtga
     8161 cctcgtcctg tccagttgtt acgagtattg ccgacgacag gcggtgaccc attccagcgt
     8221 ctcaccgcgc taaggctacg gattcgagga ctgattgatt cacgaacgcg gatctggctc
     8281 tgccctcatt accatcgcga tcgtcagcca gaatcagctc ctgcggcttg gactgcaagc
     8341 cgtcatcgca ccgcacgcgc acatccggct gatcggagag gcgacgaaca tgctgcaggc
     8401 ggtgagcatc gtcacgcaag agcagccaca gctgctcatc attgagctgg agcccggcat
     8461 ggacatcctc gggctggttc gaaaggtgaa ggcttcgctt tcgaccatac gaatcatcgc
     8521 cctgcatgga attgaagaca gccgagccgg gctggaagcc ttgtcgtccg gaatagacgg
     8581 catggtgttg accacccagc cgggagtcgt gctgcttgcc atcatcgaat atctctgtcg
     8641 gcgtccggcg aacgtggcgc gctgtcatcc ttgcgacgca agcgcgttgg agacaaccgg
     8701 ctcagcaggc ggcttcgagg atcggatccc cgttctgccg aattggcccg aggacctcac
     8761 aaaacgtgag catgacatca tcaccttgat tggagatggc ctgtccaaca aagatatcgc
     8821 ctgccgcctc agcatttgca gcacgaccgt gcgccatcac ctcaccagca ttttcggcaa
     8881 actcgatgtg cgcagccgcc agcaactcct gattcgagcg cacaaatacg gcctggtcaa
     8941 actccacgcg aatgggacag ccgctcacgc acaccttgta gcgggttcgg agcagtcagc
     9001 ctgagagcgt cggaaagctc gtccacccct ttcatcgtcg tcatgaagag caacgagccg
     9061 tgccatgagg aacctcgttg cggcacatcc agctgatatg aaccggctgc agaccagggg
     9121 agacagtggc tgacggctca ggaatcgctc tgtcgagcac ctaggcaatg actgccttct
     9181 gtttgagata ttccatgaga tcggaaaagg agccctcgcg aaggatgcgc gtgaactgcg
     9241 cctgatactt cgccatgatg ctggacccgt cgatgctgat atcattgaca agccaagccc
     9301 cggatcgccg aacgacttga aattcgatcc gtgtgtcgac ttcctgcccg gcaggagcaa
     9361 tcatgacctg tgccccgtcg gcatcgtttc cttcagcgag atagaccact tgggccgcag
     9421 aatagtctcg caggtggtcc gccagatcat cgcgtaacac ctgcacaaac agtcgcacaa
     9481 actgccggcg ctctacggca ttcgacttga tcccggtctc gcccagcgac cgttcggcca
     9541 tttcttcata gttgaaggcc cgccgaacca cctgctcgat ttcccactga cgctgcgcag
     9601 agcggctcgc atccttcaac tccgtcagga tatacaacat gtccttcatc gtgcgctcga
     9661 cgatctccgt cggcgtctgt cccgcccacg aagaggtggc atgaccggat accgccacca
     9721 acatcacagc gagtagacac acgatcaagc ccaggacgga cctcgaagtc gagggacaag
     9781 gatggctcag gctgacaaat ctgtggttca tacgttctgc cttccaataa cttctgccaa
     9841 tgatcgttta tggtaccggc cgtcaaaacc gagcgtcaag cacgtggata tacctgcgtt
     9901 tcaaccgtca aaggatcagg tactgaagga gccgactatt gcgccgaacg gtagcggcgc
     9961 gccattgaga gattttgttt tcctctctgt ggcaccttcg gtatactcgc gccatgtctc
    10021 atagacgaat gcccctcctg ctgctgagtc tctccatccc tgccctcttt tcaggctgcg
    10081 aaacaaccga caaaatgctc ggcgccgccg aacgggctgt cggtagcagc acaggaagaa
    10141 ccgtcctgga tatcgtcggc ggcaaggatc cggctgaaat cgccaggcgg cgcgtcgaga
    10201 attatggacg ggacccccaa gccctgttgc gggaccttcg ctcgatccag cgggacttcc
    10261 aagcgctcat ggcggcgttg accggagaag tcgggaaaaa gtggggcacc aaggaagtga
    10321 aactgcccga gcagaagaaa tatgtgaaat acacacagaa ttaccgcagt cgcgccatcg
    10381 tggatttcga tgcgggcaat atcctcgtcg agacgcttga cgagaaggat ccgcgggcca
    10441 gcctgaagaa tgccgtcgtc accaccctcc tcacccccaa cgatcctcgc agcgtcgatc
    10501 tatttacgga taaagaaatc accctgacca gtgacaaaga gccgtacctt ttgggtctcg
    10561 tgctggatca agccggcaag ccggtgcgca cacccgcgga agcggaacag ttcgccgaca
    10621 cactcctctc caagagcacg accacccgga aggtcgagca agaagaggga ccgaaaaccg
    10681 cccacatggt caacatctcg atggtcacga atttctcgca caaacaggca gagaaatacc
    10741 gcgcggtcgt cggacagttt gcggagcgct accaaatcag tcccagcctt gtctttgcca
    10801 ttatccgcac cgagagtaac ttcaatccct ttgccgtcag ttctgcgccg gcttacggat
    10861 tgatgcaatt ggtccccacc agcggagggc gggatgcgta ccgcaaggca aaaggtgaag
    10921 acaaggcgcc ctcgcgcgac tacctcttcg atccggacaa caacatcgaa ttgggcacgg
    10981 cctatcttaa cgtgctgacg tacgcccaat tggacgatgt caccgacctg gtctcgcgtg
    11041 aatactgcgt gatctcagcc tataacaccg gcgccggcaa cgtcttcaag acattctcca
    11101 aagatcagcg gaacgcgttg cagcagatca atggattaca accggccgca ctctatgacc
    11161 gcctccgtac cggactcccc taccaggaga cccgcgacta tctcgcgaag gtcgtcgggt
    11221 ttcgaaagca atttgtgagt ctcggcgata ccgccgctaa ataaccacgc accggttccg
    11281 acaatcctcc caaaccagtg atggttggat tcttgccttc cggcgcccgc ttggttacaa
    11341 caagtcaggc aacgggtttt tcaaggagga ccaccgcgaa ctcatcacct gacaagagca
    11401 ctgcattcgg catcctcggc gccgccttcg ccctactgtg catcgcgctg gtcccgttga
    11461 tgacggttga gatcccgccg ctcgtagact atcccaatca cctggcccgc atgcacatcc
    11521 tagccgatgg cgggcattcc ccctggcttc gccaatacta cgacatccac tgggacctgc
    11581 ttcctaacct ttcgatggat ctggtggtgc cgccgcttac ccggatcatg tcggttgagc
    11641 aggcaggcaa gatgttcatc gcgctgacat tcgcactgct ggcgggcggc acgatggcgc
    11701 ttcacgctgc cctgcatcga cgctggtctc cctggccact cctggcattt ttctttctct
    11761 ataacagtgt cttcctgtgg ggcttcctca actatctctt cgggctcgga ctggctctgt
    11821 tcgcctgcgc cctgtgggtc aggctccgca ctcgttccgc gctcctggtc gtgccgttat
    11881 tttcactcac cgccgtcatg ttgttattcg ctcacctctt cgcattcggc agcttcgcgc
    11941 tgatcgtgtc gacctatgag ctctctcaat ggtggcgcct gcgagacgag gccattcacc
    12001 ctgccaacgc cgcctgctgg aaggccgctc cggcactcat cattccactc atcatcctga
    12061 cactgacccc gaccttcaaa gtggcccccg aagactatcc cttgtggctc agaggatctt
    12121 ccccgccccc actcatctcc tttctttctc ccgtaacaaa actcgaagct ctgaaaggga
    12181 ccatccgaac cgaacaccgg ctcttagatc gcgtcaccgt cctcatgctg gtcgggctgg
    12241 tcggcatagg gttggtgcgg cgacgttgct ccatcctgcc gtcgatgttc ctcccgctcg
    12301 gcgcaaccgc gctggccgcc atggcgatgc ctaatacgat cggcacaacc gcggtggtcg
    12361 atatacgaat gccgatcgtc ttcgtcttac tcaccgtggc gagttcggat tggcgcgaaa
    12421 tcagtcgagg atggtttctt ccggtggcac tggccctgag cacggtattc gcggtgagga
    12481 tgggctacat caccgagcac tggcagaaca cggcacacca ctaccgacag ttcaaacaga
    12541 cccttgatca gctgccggaa ggagcgcgcc tgctgtcggc catcaaaatg gcctcctatg
    12601 acaactggtc tccggcagaa ggccagatac ctgagcccat gcccatggta aacctgtcct
    12661 gctggggagt catccggcgg tcggtcttcg tctcgaatct cttcgccgca ccgggccagc
    12721 aaccggtccg actcacgccg gcagtccatg cactgctgac ggtagaagaa ttcctctttc
    12781 gcgcggcgcc gattccctgg gaccagctcc gggcccaata cgactacgtg gtcgtccgac
    12841 gcacccaacg cctgcgcccc cccccctcct gccgacttcg ttccattcgg ctcaggagac
    12901 gagttccagt tttatcgtac cggaagggta cagccctaac gcacggccga cagggcaatc
    12961 ccgattgctc accgagcctt gactttccag gtgtaggacg aggtgacggc ataattccat
    13021 accgtcccca caagaatgcc tgccagggcg gacaatcccc aatgaaattg ctgcgtgaac
    13081 agatacgacg caacccccac atttccaaca gcgccaagac tacacaccag gacaaacgac
    13141 aaccaccctc gcaaccactg ccaccctcgt agccgctggt cccgataggt aataatgttg
    13201 ttcaagatga aattactcgt catcgcaagc aacgtggcaa taccctgccc ccaggcaaag
    13261 ggcaccgcca accagcgata caacacccac aacagactga agtggatcag gacgccggag
    13321 ccccccacga ggcaaaatgt caccaggcgc acaggcacaa accggccgat ggacttatca
    13381 agcagcagca tgacgtagtc ccacgccacc tgactgtcga gcttgctctc cccggcttgt
    13441 ctcgacctga attcataggg cagttctatg aaccgcaacg gatgcgggga cgaggccaac
    13501 acatccagca ggagtttaaa acccatcccg gacgtccgtc tcaccagccg gtcgaacacc
    13561 tctctgcgca tagcaaaaaa gccgctcatc gggtccgtca atccggcagg caccagtggg
    13621 cggctcaacc gcgtagccca tcgactggtc aaggcccgca tgccatccca ctcgccgatg
    13681 ctgccacccg ccatgtaccg gctcccgacc acgatgtcgt acccaccctg caataagacc
    13741 ttgagcatgt cggggagcaa tcgttcgtcg tgctgaaggt cgccgtccat cacggccaca
    13801 accggtgcgg aactcgacaa catcccttcg atacaagctg aggacaaccc ccgacgtcca
    13861 atccgatgga gacaacgcac ccgatggttg gcgcaggcct gcgcgcgcac cactgcagca
    13921 gtctggtccg gagagtcgtc gtcgacaacg ataagttccc actctacccc aaccaggcag
    13981 gcgtccagag tcgccacaag ccgggggata ttgtcccgtt cgttaaatgt cggcacgatg
    14041 atggacagga caggcccttg cactactacc ctctcaatgg ctttcgcgaa gcatcagaga
    14101 cacctgccgg ccaggatcgt gatcgacgcc agactttgtc catcaggccg agaagcagct
    14161 cgaagaagac agcgaaacgc ttgcgacaca atggtactct tacaacccga tgaccagcct
    14221 caccggcggg acccgttcgg caaggccaaa cgacaattca cctctgacat cgtccggatt
    14281 gtagtaggtt ccgaacagcc ggtcccaaat cgtgagcaac gcgcctagat ttgtccgttg
    14341 atgagctgga tctgtgctgt gatggatctg atggtatcgc ggggtcacaa acacccattc
    14401 cagccaggac gacctccagg tgacattcag atgcatccaa ttgttccgca caatgtgctc
    14461 taccatgatc acctgaaatg cccatgacga agcctcatgc agtaacggaa gggcgatgat
    14521 atacgtcagg ttgaacagag cgatatgcgg aatggtggcg cggattccag aaagccagta
    14581 cagggaggtg ggggaatggt gccaacggtg gatgcgccac accggcttgg tatgcatgag
    14641 ccgatgcacc caatagagcc cgaaatcttc gacgatatag aagagggcca acttcacgac
    14701 cgtgggcaga ttgtacaggg ccgcaggcat atagttcggg atcggaatcc gatccgtcgc
    14761 ctgtaccacg gcaaggaacg atacattgta caaacctaac gcgatcagat cccgccagaa
    14821 tacagaccgg taggcaatcg gacgcgcagg aaatcgccac tcggcgagaa aacacaggag
    14881 cccgatactc cagaacagca gatagggata cagcgcctta tccataggcc tccgttcgcc
    14941 tcccacgcct cacgaggaac ctcctacgcg cctacttccg aaactgctcc ccgccatgtt
    15001 cgccggcatc ggggcgggcg cttgctcccc ttcagtaccg ccggattttg cacttggcct
    15061 accctaccga aaacctccgc agttgcgaaa ccctttgtaa ctttccgcat ctccgctctc
    15121 gacggcgacc tgccgcatgc cgatcgacat gtttcgtaga cgcccacatc gccggcaatc
    15181 gatcggcacc attattttgc ccgttacaac tcctcgggcc aacaatcagc accgttttat
    15241 ttcacgccgt caccgtgaat agaaacggtg cactccgccc cgcatgagcg cctgcatcaa
    15301 cttttcgcac taattcaatg cgttggcaat cttcccccta ttcgcctgta agggcacagg
    15361 tattgctcta cctacgagca aaccgtggct cactaaccat gcgcaccatc cacccggcaa
    15421 atttctgccg gatagacctc aaggaggagg acaacatgaa gcgacacatc gcaacctatt
    15481 ccgcaatcgt gctggcctgc agctgggcct tactcggccc cgctcccacc caggcagccg
    15541 ccgacaacat tgagccggtg acctggagca acagtcaaaa atcttccgcc tgggcggaag
    15601 aactgctggg ccaggttgtg acctaccaaa cactcgccga gaagagcctg atcccaggaa
    15661 attttgaggc ctatgtggag cagatgcgta aagtgcgcga actgtaccgc acgggaaacc
    15721 gacgagccac ctacgacggc gtaaaccaac tcatggtcat gctggaagcc agggtcggag
    15781 gaatcgatgc gcacagcgcc gacgccttgt gggacttctg ctatcgcgtt accccggatg
    15841 aataccatgc acgcgatcgc catatccggg caaagggggc cgatgaagta aagaagcacg
    15901 aagactttat gcgggacatg gaagaaagag cgtcaatggc tttctaagcc ccattggctt
    15961 catcgaactg tggaatctgc ggcgggccga ccatccaccc gaggccgtcg gcccgccgcc
    16021 ccgcaccagt gaatggagga ttccgatgct ccatgatctt cgatgttacg atctacgctc
    16081 acgagaaccg tcctgctggt gtgtgtcatc ctgcttgaga gcacaggcgc aacccttgtc
    16141 ttgtccccgg tgtgggcaga aagcgaacgc gccggatatg ggcctgcctc accaggcttg
    16201 tcatcgtcac ccgtgcgcct tcggtttgaa ccggatgacg cggtatcctc gcccctcggg
    16261 tcgtccttcg atctccagca tgtctggatg cgcgcggacc tccgcgtgag acctgagtgg
    16321 cgcaacggcg tctgctttgg aggcggcccc ccgatcgccg gcgcctgcaa cagtctcgat
    16381 cagttcgggt ccggcacagg agctcatagc ggtcgtcatg ccggcgattt cttcatccag
    16441 cagtgggcac gcattggcct gggctatgat ccttccccca atttaaattt ttatgtcgag
    16501 ctacaagact ccgccacatg gggggggaat ggcgacccca ccgaaaggaa tcaagtgggt
    16561 gacgcgagca ctcataattg tgggatccag ctagcgggac aatgccgctt aggcattcgc
    16621 gccggctata tgctggtcag gaacatcgcc ggtatcgatg ggttcagcgc caagatcggc
    16681 cggcagtatg tcgtcttcgg caaccaacga ctcttcggcc atttcgactg ggccaatacc
    16741 ggctattcgc acgacggcat catgctgagt tatgcgaccg ccgccttcga tacgaaactg
    16801 ggctggtttc gaaattccga aacggacttg gggcaggcga gcccgggtgg aagccttacg
    16861 ccaaacctgt tgacatgcaa cccggcggca aacgcctcgg ccacctgcaa tccggccctt
    16921 gcgcaacatg cgaccgacgc gggcagcgac gtggatctgg tggtgttcta cagtcaactc
    16981 cgcagcatcc cccgcatgat cgtagagccc tactatgtcc tgtactcgaa ccgtcttccg
    17041 gagcgggcca atcccggcca atacttaccc aaatcggcct ctcagttacg gcacatggtc
    17101 ggcctccgca ttgagttccg ggacggcaat tgggatttca cccatgaagc ggcctatcaa
    17161 ttcggccgta gcgccgatgg attcgatttc gacaaccggc gcaatctgcg tatcaatgcc
    17221 tgggcttccg ggacgtggct cggctacaca tggtataccc atcattggaa gccccgggtc
    17281 gccatcggat tcgactacgc ctccggagac ggcgacagca actgtgtccc ccccggcgga
    17341 aacctggcac gcagttgcgg cggcaatgcc aacacattcg agaatttttt cccgaccaat
    17401 tttctgcacg tcggctacat gctcaacggc gcctggcgca atagcgtcca gccgcaggtg
    17461 aatgtccagg ctcgaccgac cgctcgagac catatcgaac tctgggcgct gcgcaagtac
    17521 ttggcaagcg cccgagacaa ttggtaccgt ggatcacaag ggccgttgat tttctccgcg
    17581 tccgacaacc ggacgactca catcggcgat gaactcgaca tcgcatggag ccatatgttt
    17641 gcggacggta cggtctcact gtcaatcatc tacggccact tcttttcggg ctcctatatc
    17701 aagcaccagc tcggtaccgc cgctgaccag gactggggca ttgtgcaact ctggacgaat
    17761 ttttagacga ctatgcaacg cgccacactg aattttgaaa cgctgctgga agtaaccaac
    17821 gccctgaac
//
LOCUS       DAORQN010000021        17510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_215_length_17510_cov_16.520137, whole genome shotgun sequence.
ACCESSION   DAORQN010000021 DAORQN010000000
VERSION     DAORQN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_17510_cov_16.520137"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..>226
                     /locus_tag="PK614_02785"
     CDS             127..>226
                     /locus_tag="PK614_02785"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81165.1"
                     /translation="MHVEFIDCRDYRAVAQAIRDLKVRGAPAIGVTA"
     assembly_gap    227..236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <237..1107
                     /gene="mtnA"
                     /locus_tag="PK614_02790"
     CDS             <237..1107
                     /gene="mtnA"
                     /locus_tag="PK614_02790"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRB81166.1"
                     /translation="AMGVALGAQAVTSIEYRDFAKQVGEICDHLAASRPTAVNLFWAI
                     GRMKQKLVALNDQPVARIKAELIIESQTILDEDIALCKAMGQHGAGLIQSGQTILTHC
                     NAGALATAGYGTALGVIRAAWEQGKQIQVIADETRPVLQGARLTAWELMQDQIPVTLI
                     TDNMAGALMRQGKIQICVVGADRIAANGDVANKIGTYSVAVLARAHNIPFYVAAPYST
                     IDLKTQSGAEIPIEERNPLEVTAIHGSHPVAPPGVAVFNPAFDVTPAGLITGIITERG
                     VFKPGELATHFRS"
     gene            1295..1714
                     /gene="ndk"
                     /locus_tag="PK614_02795"
     CDS             1295..1714
                     /gene="ndk"
                     /locus_tag="PK614_02795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRB81167.1"
                     /translation="MSERTLAIVKPDAVKKNAIGDIINRYEKAGLRPVAMRLMHMSKA
                     TAQGFYAVHKARPFFDSLCTFMSSGPCVVLVLQGDNAIKKNRELMGATDPAKAEQGTI
                     RAAHGANIEFNAVHGSDAQETAKFEIGYFFAEMELVG"
     gene            1722..3992
                     /locus_tag="PK614_02800"
     CDS             1722..3992
                     /locus_tag="PK614_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81168.1"
                     /translation="MSAVKSYVMHARRSWPGRRAGSPLFSLILLMVFVAGCAGSQPTS
                     VPSGAPPTETPAVAAETSLWYQASTAFSDGRYSAAIHLYERYLTNYPKSRRAVEAHWD
                     LGQSYEQMGEVTAAIKEYRTLVGPEGAPPSAQNSYAERAQHRIDALRNQPASPSGARS
                     GHTALYVSFSNLPPMSQVEAWIRQLSAQGISAILMDASLESSFTRPLIPTSQPASSPL
                     PNLRSGTSLPKWSWSGRRTLSAVKSYVMHARRSWPGRRAGSPLFSLILLMVFVAGCAG
                     SQPTSVPSGAPPTETPAVAAETSLWYQASTAFSDGRYSAAIHLYERYLTNYPKSRRAV
                     EAHWDLGQSYEQMGEVTAAIKEYRTLVGPEGAPPSAQNSYAERAQHRIDALRNQPASP
                     SGARSGHTALYVSFSNLPPMSQVEAWIRQLSAQGISAILMDASLESSFTRPLIPTSQP
                     ASSPLPNLPGGALFATSRGPVLTDWYGVMVPQAHELGISVYAVLDLYHAPLSDHRPES
                     KMLLYDPTRRKVHPWTQFDLLAPAVQQQMAQLLADLLKTGVDGLVFRSRAENSFAYEV
                     SDGVLRQFETQVHQPSSEVAQALRDRMALRPNEPAPASDKTLWRWVGWKARQELDTLA
                     RFKKYLQKAVPRFRMVLEIHPEALSNPTSALVNYGEDAAEARRRGFDLLLGGPSREAS
                     DVRSFGGSYKGWSRDVVQSEKGEPQTLPQGWVLLRTPAEHGGGMFTGLAQQADELRTP
                     DIRHLVFVPDAPVVIP"
     gene            4088..4474
                     /gene="gcvH"
                     /locus_tag="PK614_02805"
     CDS             4088..4474
                     /gene="gcvH"
                     /locus_tag="PK614_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRB81169.1"
                     /translation="MIPSNLRYHQEHEWVRAEGAQATVGISHFAQDALGDIVFIDLPK
                     VGAKVTAGQQIGEVESTKTTSTIYTPVSGTIAKINADLKDHPEVVNSDPYGKGWMAVI
                     DLTAPAEVDQLMTAAQYETFLSQQKH"
     gene            4482..5879
                     /gene="lpdA"
                     /locus_tag="PK614_02810"
     CDS             4482..5879
                     /gene="lpdA"
                     /locus_tag="PK614_02810"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRB81170.1"
                     /translation="MKHLAILGAGPGGYVAAIRAAQLGARVTVIENQALGGVCLNWGC
                     IPSKALLSVVEDFGIQLSGPVTYDPTVMVARKNKVVSTLVKGIATLFKTWNIDHLEGT
                     GELLDARTIRVTKADGVETRVMADGLIIATGSSWPNLPLFPIDGTQIITSKQALDLSR
                     IPASLLIVGGGVEGCEFASLYSGLGTQVTLVELVPRLLPLEDEEISQMMERELKKRGV
                     DIRTGVTVDQIVRQPEVVTAHLRDGLSLNVEQVLVSVGRGFNSRGIGLEKAGVQVGTR
                     GEIVVNDRMETNVPGVYAIGDVVGKAMLAHVASAQGKVAVENFIGHPRTIDYDVIPTG
                     IFTLPEIGRVGLTEQQARDRCAAAGKDPQQVVRVGRFRYGGLGKAQATGDIQGLVKVI
                     GDADSDRILGVHILGAHATDLIHEAALAMHLGATVSRVAEMIHAHPTLAEGLMEAMED
                     VHGHAIHLARKPSSS"
     gene            5876..6418
                     /locus_tag="PK614_02815"
     CDS             5876..6418
                     /locus_tag="PK614_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB81171.1"
                     /translation="MMRSLLIKLAMLAVTLGSLVWIGSVTPVRQIPSRPAQPVAAVPG
                     PAAVRQVSIAPPQVVSPADGSTLVANVPDVPAAPAVEESEPLVRQEQPANSLRRVHRE
                     VARQVDLNHATASDLQALPGIGPKLAQRVIDHRTARGPFGKIEDLQQVKGIGRKKLDR
                     LRPHVLVTNTRSLARHKGTL"
     gene            6415..7632
                     /gene="tyrS"
                     /locus_tag="PK614_02820"
     CDS             6415..7632
                     /gene="tyrS"
                     /locus_tag="PK614_02820"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRB81172.1"
                     /translation="MTSVAQQLDLILRGIVEVINPNELESKLARSLKEQRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHQVIFLIGDFTGMIGDPTGRSETRVALSKEKVL
                     ENAKTYERQIFKILDPHKTQVEFNSRWMSAMTADGLIELSAHYTVARMLEREDFHKRY
                     TEGKPISIHEFMYPLIQGYDSVALQADVELGGTDQKFNLLMGRDLQRDYGQDAQVVIT
                     MPLLEGTDGVRKMSKSLGNYIALEDKPDEMFGKVMSISDALMHRYYELLTTEDLERVK
                     TAHPMDAKQSLAELIVARYHGAEVGRESRAMFQQKFQSREFPDQPDARVTLTPSDVKD
                     AADPMMGIVDLVMKTGLVPSKSEARRLIVQGGVEINEKKLMNVNASVALSVGKPLHMR
                     VGRRKFAVAEYKV"
     gene            7701..7775
                     /locus_tag="PK614_02825"
     tRNA            7701..7775
                     /locus_tag="PK614_02825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7733..7735,aa:Gly,seq:ccc)
     gene            complement(8367..9074)
                     /locus_tag="PK614_02830"
     CDS             complement(8367..9074)
                     /locus_tag="PK614_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81173.1"
                     /translation="MNGIVEIKTTTVVTIVTNNSLLRLGLQKIVDDEKWVRLVGHNCH
                     AGNLDELLAKERPHIVILDTEIGVTTPEIIQTIKNMAPGIKTILLSGFDDTECTRQAF
                     ASGVDGIVLKVQPSAVLLATINYLSHTGGEVGAPISHSPAPLKLGHTPAITPPPPPSP
                     AQAKWPDGLTEREREVVRLISEGLSNKDIADRLCISSITVRHHLTNIFDKLGVSNRQK
                     LLIHAHQQGLAGPPALA"
     gene            10233..10514
                     /locus_tag="PK614_02835"
     CDS             10233..10514
                     /locus_tag="PK614_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB81174.1"
                     /translation="MKKLRFTEDQIRKILSSAGGRNTIHAICRKQRISKSTFYRWRSQ
                     LRAHDEASQTKRLLDLEMENGQLKRQVAELLLDYNALRVALVNEASSAC"
     gene            10587..12014
                     /locus_tag="PK614_02840"
     CDS             10587..12014
                     /locus_tag="PK614_02840"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB81175.1"
                     /translation="MIRYFSRICARAFFVLALILGAVLLIASEGECQQISAITPPTAG
                     TSLLGGQGYMWLSQFANPGVLGALYQTPTFPWTGIYPLYPGSFTAIAPDDGFRAGPLR
                     LHPFMGMAEMFTDNVLRTNSKRSDFFTTLAPGLQAQLPFGTRHLFVADYRTNLQFFHR
                     TPSNNVQDQTASAMTRFEFASGLKLDLQGEHKLGHDPRGSALDLQTLELNKWVANSIT
                     GRAQYEGGQVGATLTMQTMRWNYINNNQGLIRDRLTNYLGTTFSAKVLPHTSALLNLS
                     VVQEIYDENKNLDSAMYTISTGARWEISGNTSGQFLVGYQHLKFTRAQVNQPGPVLSI
                     FRRDQDSFNNLFAMGQLQWTPLANLNLALQPYRTIQQTVVAGTIFFTATGVNLSATYP
                     LGMRFEVTSNLGFEVDRFSSPAGALALTPTRSDTLKNAAIGLNYRAVRWLGVSLQYVF
                     EDRSSNARDFNYQANTAMLSVQGMF"
     gene            complement(12111..13628)
                     /locus_tag="PK614_02845"
     CDS             complement(12111..13628)
                     /locus_tag="PK614_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81176.1"
                     /translation="MMKASRQIVLVGLLMIGCTFGIGKPLHAQSLAQPPAPQSPHWPH
                     INLFAYARSLFLANLSNDTYQWYVSHLDAVEVQGNGPAVRTLNPTSELFSYQFDLSTY
                     TSAALVGVPEEAFLHFSESTVLTFYSLDRKQVVGTATVAGCPENTPLSSGCRVQTHLW
                     NDRRYVFNPQSTAFRAWKAAKILGPLNGYGYATDTSRFIWIDEHAPGFSWPLSIGYQT
                     IVQSGGGIREFAGRRPGNSSFESEYANAVTDWLSFLAIQAAQVSKKVLINANENALHP
                     WLLGQANAIHGISTESKHRPDGFSGPAEYQQYLTLIKGLTTAGGLVDLHGIWCYTGPA
                     EYSSGNYGSSAARYRMWRLASYYQFKEPAGSPGTVYFNPGFCSDETPTIDQSSKDQSE
                     WLLAYQVDVGQPSGDTILYQQGTAGCSYQIFGRSFTKALVLVRPKDGPTCTDFSDASA
                     ATVTFTTPIQTLLENGTLSAPTTIVKIRNAEAVIAYQVGGVDTTPPSQPFGVTVR"
     gene            13886..16072
                     /locus_tag="PK614_02850"
     CDS             13886..16072
                     /locus_tag="PK614_02850"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRB81177.1"
                     /translation="MNTRKVDQESYLIDLLRVLKQRWWIIILCSMCCAAIMALWSHVQ
                     APGFESTTTVLIEQEHQGPLQKSDRTMADFSPDYYETHFELLKSRRVLEKAAEELKLL
                     ERSEYLTFAGLYENLLITVQTTFGSKTTTTSSTPPPLASGERTNESWDLALGRFEQTI
                     QIKPVRGTRLAKITVKSGDAHFAAEAANAIARAYIHLNEEVHAQKKERAREWFSSHLA
                     DLRNKVEQSQEALNAYRVKYGVVETGDRQTPSRQRLAELSSELVKLELRRAEAESRYQ
                     QIALLMPEKTKAEGIDWAKWGDHAEVLSSPLIQVLRTQEIKASSEVAELAEKYGSLHP
                     KIARAEAELHQLRERVVEEVRKIYASIRHERDLATTRESAIRVAVDRQKRDVMQFDQY
                     QAQQGILEREVKSAQHVYDLFLGEAKEASLASELVATNVVIADPATPASKPSRPRKVA
                     NTILGLLSGLVFGLGIAIGLDHFDQSLKGPRDLASYLPTIAFLGAIPRVKHLETQEPM
                     ILRGPRALPALEAFRSIRTSIMLSGMIPDHGSLVITSSGPDEGKSTVAVNLAFAMAQI
                     EDGRVLLIDADLRSSHPGSLFLLKGDGIRGLGDFLDGDAEFDDVVHHSGESNLWTIPP
                     GKVTTNPSELLSSRRMKLLLEKCRTAGYFVIMDTPPVTLFSDAVILGSRADGVMLIVG
                     AGQADREACQLAGHRIVQAGGRLLGVVLQKAMGPRLSHYSQYAQIQ"
     gene            16115..17461
                     /locus_tag="PK614_02855"
     CDS             16115..17461
                     /locus_tag="PK614_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRB81178.1"
                     /translation="MSQADLNPASVPMAPIETGIRFSHGVASEIASPAPSIRRSALWT
                     GLGDAFYALCQWGILVILARMGTPEMVGQFALASAITAPVIIFANLGLRQLQSTDARN
                     QFQFRDYVAMRLASTVLALATIAVLAFQYRVDTALTILVLGIAKCFEAISDVFYGLLQ
                     KHERMDLITISLLLRGVTSAAGFAVGFILLGGVLGGVVGLAMGGRLVLIAYDIPRGSQ
                     ILSLTAHKPHTAGIWRQSQMQALCALALPLGVGTMLLSLNANIPRYFIERYIGDGALG
                     LFAGMAYVAAAAGMAVNSLGQAMSPRLSQHWARGAFVEFRTLFMKFAALAGLWGLIGI
                     GVAFAVGAQLLTLLYGADYATHSDTFVWLMGVAAVSHLASVCGFGMTAARIIRSQSIQ
                     FACVAAIGTVSAIVLIPGHGLNAAALAFGISLFAQLFISALCLHYALARKDQPCKS"
BASE COUNT         3777 a         4746 c         5060 g         3917 t
ORIGIN      
        1 atttttctgc cgtcctgaga tgacgccgga ttccttctct ttacgtaacc ggaggacatg
       61 cctatggtgc ccaccgtcga atggaaagac ggagcggttc gcctgcttga tcaaagccgc
      121 cttccgatgc acgtcgaatt catcgattgt cgggactatc gcgcggtggc gcaggcgatc
      181 cgagacttga aagttcgtgg cgcgccggcg atcggtgtca cggcagnnnn nnnnnncgcc
      241 atgggtgtgg cgctgggcgc gcaggcagtg acgtccatcg agtatcgcga ctttgccaag
      301 caggtggggg agatctgcga ccatctcgcg gcatcgcgcc cgaccgccgt caatctgttt
      361 tgggccatcg gccggatgaa acagaagctt gtggcgctca acgatcagcc ggtcgcgagg
      421 atcaaggccg agctcatcat agaatcgcag acgattctgg acgaggatat cgccctctgt
      481 aaggctatgg ggcagcatgg ggccgggttg atccagagtg ggcagaccat tctgacgcac
      541 tgcaacgccg gcgcccttgc gaccgccggg tatggaacgg ccctgggtgt cattcgggcg
      601 gcctgggagc aggggaagca gatccaggtc attgccgacg aaaccagacc ggtcttgcag
      661 ggtgcgaggc tcacggcatg ggaactgatg caggatcaga ttccggtcac gctcattacg
      721 gacaacatgg ccggcgcctt aatgcgccaa gggaagattc agatctgtgt cgtcggcgcc
      781 gatcggatcg ccgccaacgg cgatgtggcc aataagatcg gcacgtattc ggtggccgtc
      841 ctcgcgcggg cgcacaacat tcccttctat gtggcggcgc cgtactctac catcgatctc
      901 aagacgcaat cgggcgccga gattcccatc gaagagcgca atccgcttga agtcactgcc
      961 attcatggca gccatcccgt ggctccgccc ggcgtggcgg tgttcaatcc tgcctttgac
     1021 gtcaccccgg ccgggttgat taccgggatc atcaccgagc ggggcgtgtt caagccgggt
     1081 gaactggcca cgcacttccg atcgtaagag gcaggctacg cctctttccc gcctcgctga
     1141 acgtgagcgg gtcgcctgag cggcgaatgg ccatccggca tttctggtcg tcctttacct
     1201 tgccagtcat tccgacgctt ctttataatg ccgcgagacc gtgccttacg cctcaatgaa
     1261 tgtcgatttt ttagctgaag gagcctgacg acccatgagt gaacgaacgc ttgccattgt
     1321 gaaaccggat gccgtgaaga aaaacgcgat cggcgacatt atcaatcggt atgaaaaagc
     1381 gggattgcgg cctgtggcga tgcggctgat gcatatgtcg aaagccacgg cccagggatt
     1441 ttatgccgtg cacaaggcgc gtccgttctt cgacagcctc tgtacgttca tgtcgtccgg
     1501 cccctgtgtc gtgctggtgt tgcagggaga caacgccatc aagaagaatc gcgaactgat
     1561 gggcgcaacc gacccagcca aggccgagca gggaacgatc cgtgcggctc atggagcgaa
     1621 cattgagttc aacgcggtgc acggctccga tgcgcaggag acggccaaat ttgagatcgg
     1681 gtacttcttt gccgaaatgg agttggtcgg gtaggcgcac gttgagcgca gtcaagtctt
     1741 atgtgatgca tgctcgacgg tcctggccgg gccgtcgagc cggctcgcca ctattttctc
     1801 ttatcctgct catggttttc gtagctggtt gtgccgggtc gcagccgacc tcggtgccct
     1861 ccggcgcgcc gccgacagaa accccggcag tcgcggcgga gaccagcctc tggtaccaag
     1921 cctccaccgc gttctccgat ggacggtatt ccgccgccat tcatctctac gaacgatatc
     1981 tcacgaacta tccgaaatcc cgtcgcgcgg tggaggccca ttgggaccta ggacagtcct
     2041 acgaacaaat gggtgaagtg acggcagcga tcaaagagta tcggacgttg gtcggtcccg
     2101 agggtgcccc tccctccgcg caaaactcct acgccgagcg cgctcagcat cggatcgacg
     2161 ccctgcgcaa ccagccggcc tctccgtccg gcgcgagatc aggtcacacc gcgctctatg
     2221 tctccttcag caatctgccg cccatgtcgc aggttgaggc ctggattcgt cagttgagcg
     2281 cgcagggcat cagtgccatt ctgatggatg ccagtctgga gtcatccttc acccggccgt
     2341 tgattcctac cagccagccg gccagtagcc cactcccgaa tttgagatcg ggtacttctt
     2401 tgccgaaatg gagttggtcg ggtaggcgca cgttgagcgc agtcaagtct tatgtgatgc
     2461 atgctcgacg gtcctggccg ggccgtcgag ccggctcgcc actattttct cttatcctgc
     2521 tcatggtttt cgtagctggt tgtgccgggt cgcagccgac ctcggtgccc tccggcgcgc
     2581 cgccgacaga aaccccggca gtcgcggcgg agaccagcct ctggtaccaa gcctccaccg
     2641 cgttctccga tggacggtat tccgccgcca ttcatctcta cgaacgatat ctcacgaact
     2701 atccgaaatc ccgtcgcgcg gtggaggccc attgggacct aggacagtcc tacgaacaaa
     2761 tgggtgaagt gacggcagcg atcaaagagt atcggacgtt ggtcggtccc gagggtgccc
     2821 ctccctccgc gcaaaactcc tacgccgagc gcgctcagca tcggatcgac gccctgcgca
     2881 accagccggc ctctccgtcc ggcgcgagat caggtcacac cgcgctctat gtctccttca
     2941 gcaatctgcc gcccatgtcg caggttgagg cctggattcg tcagttgagc gcgcagggca
     3001 tcagtgccat tctgatggat gccagtctgg agtcatcctt cacccggccg ttgattccta
     3061 ccagccagcc ggccagtagc ccactcccga atttgcctgg cggtgcgttg tttgccacgt
     3121 cgcgtgggcc cgtgctcacg gactggtatg gggtcatggt gccgcaggcc catgaactgg
     3181 ggatttccgt ctatgcggtg ctcgatctgt accatgcgcc gctgtccgat catcgcccgg
     3241 aatccaagat gctgctctac gacccgacga gacgcaaggt acatccctgg acgcagttcg
     3301 atctcctggc tccggccgtc caacaacaaa tggcgcagtt actggcggat ctgctgaaga
     3361 cgggcgtcga tggtctggtg ttccgcagcc gcgccgagaa tagtttcgcc tatgaagtga
     3421 gcgacggcgt gttgcgccag ttcgaaacgc aggtgcatca gccttcctcc gaggtggccc
     3481 aggcccttcg cgaccgcatg gcacttcgcc cgaacgagcc ggctccggct tccgacaaga
     3541 cgctctggcg ctgggtcgga tggaaggcgc gccaggagct cgatacgctg gcccgtttca
     3601 agaaatattt gcaaaaggct gtcccgcgtt ttcggatggt gctggaaatt catccggagg
     3661 ccttgtcgaa tccgacgtcc gccctagtga actacgggga agatgcggcg gaagcccgtc
     3721 ggcgcggatt cgatctcttg ctgggcggcc cgtcccgtga ggcgtcggac gtgcgctcat
     3781 ttggcggttc atacaaggga tggagtcgcg atgtcgttca gtcggagaag ggcgagccac
     3841 aaacccttcc gcaggggtgg gtgttgctcc ggaccccggc ggagcatggg ggcggcatgt
     3901 tcacgggact cgcgcagcag gcggatgaac tgcgcacgcc cgacattcgc cacctggtgt
     3961 ttgtgccgga cgcgccggtg gtgattcctt gacaaaggtt ttcgcagctt ttacgatcgc
     4021 gcatcccgtt tcctgcgcgc ttttattcac gtaggccgct cgtgcagcgg ccgtcacgag
     4081 gagagtcatg ataccgtcca atcttcgcta ccaccaggaa catgaatggg tccgtgctga
     4141 aggggcgcag gcaaccgtcg gcatcagtca ttttgctcag gatgcgctgg gcgacattgt
     4201 gtttattgat cttcccaagg tgggcgcgaa agtcaccgcc ggccaacaga tcggcgaagt
     4261 cgaatcgacc aaaaccacct ccacgattta taccccggtc agcggaacca ttgcgaagat
     4321 caatgccgac cttaaggacc atcctgaagt cgtgaattcc gatccgtacg gcaagggatg
     4381 gatggcggtc atcgacctca ccgctccggc tgaagtggat cagttgatga cggccgccca
     4441 gtacgaaaca tttctaagcc aacagaaaca ttaggcgcgt cgtgaagcac ctcgcaattc
     4501 ttggtgccgg tcccggcgga tacgtggcgg cgattcgagc ggctcagctc ggggcgcgtg
     4561 tcaccgtcat cgaaaatcag gctctaggcg gagtctgtct gaactggggc tgtatcccga
     4621 gcaaggcatt gctctcggtc gtggaggact tcggtatcca gctgagcggg cctgtcacct
     4681 acgatcccac tgtcatggtg gccagaaaaa ataaagtcgt ctccacgctg gtgaaaggca
     4741 tcgccacgtt attcaagacg tggaacatcg accacctgga aggcacgggt gagctactgg
     4801 atgcgcgtac aatccgtgtg acgaaagcgg atggcgtcga aacccgggtc atggctgacg
     4861 gcctgatcat tgcgaccggt tcgtcctggc cgaatctccc gctgtttccc atcgacggca
     4921 ctcaaatcat caccagtaag caggcgttgg acctcagccg cattcccgca agcctgttga
     4981 tcgttggcgg gggcgtggaa ggctgtgaat tcgcctccct atacagcggg ctcggtacgc
     5041 aggtgacgtt ggtcgaactc gtcccgcgtc tgctgccgct ggaggatgaa gagatcagtc
     5101 agatgatgga gcgcgagcta aagaagcgcg gcgtggacat ccggaccggc gtcacggtgg
     5161 accagatcgt ccggcagccg gaggtcgtca ccgcccatct gcgcgacggc ctgtcgctca
     5221 atgtggagca ggtgctggtg tcggtggggc gggggttcaa ctcacgtggc attggattgg
     5281 agaaggccgg agtgcaagtc gggacgcgcg gcgaaatcgt ggtcaatgac cggatggaga
     5341 caaacgtgcc cggcgtctat gcgatcggtg atgtggtcgg gaaggcgatg ctggcgcatg
     5401 tcgcatcggc gcagggcaag gtggccgtcg aaaattttat aggccatccc cggacgatcg
     5461 actacgatgt catcccgacg ggcatcttta cgttacctga aatcgggcgg gtgggactca
     5521 cagaacagca ggcccgcgac cggtgtgcgg ctgctgggaa ggatccgcaa caggtggtgc
     5581 gggtcggacg ctttcgctac ggcggattgg gcaaagcgca ggccacaggc gatattcaag
     5641 gattagtgaa ggtgattggc gatgcggaca gtgaccggat tctcggggtg cacattctcg
     5701 gagcccatgc caccgatctc attcatgaag ctgcgctggc aatgcacctg ggcgcgaccg
     5761 tctcacgtgt ggcggagatg attcacgcgc acccgaccct ggccgaagga cttatggagg
     5821 cgatggagga cgtccatggc cacgcgatcc acctcgcacg gaaaccatcc tcctcatgat
     5881 gcgctcactc ctcatcaagc tcgcaatgtt ggccgtgacc ttgggatccc tggtctggat
     5941 cggctccgtc acgccggtca ggcagattcc gtcgcgtcct gcccagcctg tggctgcggt
     6001 tccaggtccg gcggcagtcc ggcaggtttc gatcgcgccc ccgcaggtgg tgtctccggc
     6061 cgatggatcg accctcgttg cgaacgtccc cgatgttcct gccgctccag cggtcgagga
     6121 atccgaaccc ctcgtgcgac aggagcagcc ggcgaacagc ttgcgtcggg ttcatcgtga
     6181 ggtagcacgg caagtcgatc tcaatcatgc caccgcgtcg gatttacagg ccttgcccgg
     6241 tatcggtccc aagctggcac agcgggtgat cgatcaccgg accgcacggg gaccattcgg
     6301 taaaatcgaa gaccttcagc aggtgaaagg catcggccgg aaaaagctcg accggcttcg
     6361 tcctcacgtg ctcgtcacca atacccgatc gctggccaga cacaaaggaa cgctgtgact
     6421 tccgtagcac aacagctgga tctgatactt cgagggattg ttgaggttat taatccgaac
     6481 gagctggaat caaagttggc tcgctccctc aaggagcagc gaccgctccg ggtcaaggcg
     6541 ggcttcgatc ccacggctcc ggacttgcac ctcgggcaca ccgtcctgat ccacaagttg
     6601 aagcactttc aagacctcgg gcatcaggtt atctttctga tcggtgactt caccggcatg
     6661 atcggcgatc cgacgggacg gtcggagacg cgcgtcgcgc tctcgaaaga aaaggtattg
     6721 gagaacgcga agacgtacga gcgccagatc ttcaaaattc tggaccctca caaaacgcag
     6781 gtggagttca acagtcgttg gatgagtgcg atgaccgccg atgggttgat cgaattgagc
     6841 gcgcactaca ccgtggctcg catgttggag cgggaagact ttcataagcg gtacacggag
     6901 gggaagccga tcagcatcca tgagttcatg tatcccttga ttcaggggta tgactccgtc
     6961 gcgctccagg ccgacgtcga actgggcggc accgatcaga aattcaatct tctgatgggg
     7021 cgcgacctgc agcgtgacta tgggcaggac gcgcaggtcg tcatcaccat gccgcttctg
     7081 gaaggaacgg acggcgtccg gaagatgagc aagagcctcg gcaactatat tgcgctggag
     7141 gacaagcctg acgagatgtt cggcaaggtc atgtcgatca gcgatgcgct gatgcatcga
     7201 tattatgaat tgctgacgac ggaagatttg gaacgcgtga agacggctca ccctatggat
     7261 gccaagcaat cgctggcgga gctgatcgtg gcccgttacc acggggctga ggtcggtcgc
     7321 gaatccaggg ccatgtttca gcagaagttt caatcgcggg aatttccgga ccagccggat
     7381 gcccgtgtga ctctgacgcc ctccgatgtc aaagatgccg cggatccaat gatgggtatc
     7441 gtcgatctag ttatgaagac cggcctagtg ccgagtaaaa gcgaagcgcg gcggttgatc
     7501 gtgcagggtg gcgtcgaaat taatgaaaag aaactaatga atgtgaacgc gagtgtggct
     7561 ctgtcggtcg gaaagccgct ccatatgcgc gtcggtcgcc gtaaatttgc ggtggccgaa
     7621 tacaaggtgt agcggcgctc gccggttgcc ttgacttaac agcggttccg gcgtaggatt
     7681 cgattgctca ttcgcaaggg gcgggcgtag ctcagtggta gagtccttgc ttcccaagca
     7741 agttgtcgtg ggttcaaatc ccatcgcccg ctccatacct tgcttgcccc gaccgggaac
     7801 cctccctgct cattccaggc gaccggataa acccctctag taactttctc ttcaggccaa
     7861 tccacatgca gcagatttga acccacgaca gagattctta gggcaagtgt tccctcaatg
     7921 gggagaggca ttgttcccgg cgtggagcca cacaggccgg tgctcccgct tcctagctta
     7981 cctgtccgac gggtggccat ttttattgtt tcctaggccc caaccgaatt aggaattgcc
     8041 cgttcctaat tctgcctgcc acgccgcata ccctatgtca attggcattg tcggtaatcg
     8101 gatccggtgc actgacaatc cagcttcgat tctctacctg attcggcggt tcattcatct
     8161 gaatttcttc ccattgtccg gggggagcga ctgtgtggtg acgcggatca ggcctgagta
     8221 ccgggaagca ctgtgtgcac aggctgcacg agtcgctttc cggcactatc tcaagggcgc
     8281 gacggctgga ggaccacagc gcagccggca ggcgcgttgt ttgcgacgtt aagcagagag
     8341 aacagcaaga tgattcgggt ttgcgactag gccaaagccg gcggtcctgc gagcccctgt
     8401 tggtgcgcat ggatgagcag cttctggcgg ttggacacgc ccagcttgtc aaaaatatta
     8461 gtgagatgat ggcggaccgt aatactggag atacaaaggc ggtcggcaat gtctttattg
     8521 gacaggcctt cactgatcaa gcgcacaact tcgcgttctc gttccgtcaa gccgtctggc
     8581 cacttcgctt gagcgggcga tgggggaggc gggggggtga tagccggggt gtggcctaat
     8641 tttaatggag ccggagagtg gctgatcggc gccccgactt caccgcctgt atgtgagagg
     8701 taattgatgg ttgcgaggag cacggcagat ggctgtactt tgagcacgat gccatcgacc
     8761 ccggacgcaa atgcctgccg ggtgcattcg gtatcgtcga atccgctgag caggatggtc
     8821 ttgattcctg gtgccatatt cttgattgtc tgaatgatct ccggtgtcgt gactccgatt
     8881 tccgtatcga gaatgacaat gtgcggccgc tcttttgcga gcagctcgtc aagattgccg
     8941 gcgtgacagt tgtgtccaac caatcgaacc catttctcgt cgtccacgat tttctgcagg
     9001 ccgagccgta gaagagagtt gttggtgacg atggttacca cggtggtagt ctttatttct
     9061 acgattccgt tcatgaatcc tcccgaatca gtgctgccca gcgagccgcc gagtcccaca
     9121 ttcagaagca cctgcgtagt taataaaatt actacgtaat cagtgctaag gtgaatatgt
     9181 gatggaatgt tcctacacgg cgcatattcg catatctctt tgtcagtagg caatgagcaa
     9241 atctcactaa tagattcgcg gcaaatcatt gcggggtggt ggtttgcaag gatgatcctc
     9301 gcagctttca tggaatgatt aaagtgttca aaagtgtaca tttgtgcttg tacgagtctt
     9361 gtgactagtg ttatttcgag gttttttgcg tcgaagaacg cgggacatga atctggaatt
     9421 gaagaagaat aaatacccgt attcgttgtt gtccgtctgt gcacaaggcc atggattagg
     9481 gggcacaaag gtgctgattg gaaaaaatcg tgactccaga agaagagcac ttctgtccga
     9541 tgtaaaccgg tctgaaagtc tttagaaggg tacaggtact gatgggactc agagggcgaa
     9601 gctatgttga acacttttca atccgactct caccgtgtga attcgttatc gagagacacc
     9661 gctgtcgatc ttcactggta tgccataagt actcgctcac ggcatgagaa gttggtgagg
     9721 gatcgactcg cctccatcgg tgtcgagcca ttgctgccgt taacgaggca gccgcgccag
     9781 tggtcagatc gaaaagtcat gacagaattg ccgctctttg ctggatactg ttttgcacat
     9841 ttctctctga acaattgtcg ggcgattctg caacttcctg gcattgtggg catcgtgggc
     9901 gcgacagctc ctgaggctat tcctgcccac gagttagagg cgatcagggt gctctctgcg
     9961 atcagtcgac ccatagagtc gcatgattac ttttcggagg ggacatgcgt cgaagttgta
    10021 aggggtccat tgattggcat gcgagggcag ttcgtccgtc gcgatggacg gcatttcatc
    10081 gtgttgcgtg tgaacttgat tcagcaggct gccgctgtcc acatacatat ggacgaagtc
    10141 gctaccatca gctaaatcat acccctattg ttcctcccca tcgttctctt gcagtcatcg
    10201 ctactcgcat caaatcgacc gtaaggatac ccgtgaagaa attacggttt acagaggacc
    10261 aaatacgcaa gattctgagc tcggcaggtg gtcgaaatac gattcacgcg atctgtcgca
    10321 agcagcgcat ctcaaaaagc acattctatc gatggcgttc tcagttgcgc gcgcacgacg
    10381 aggccagcca gactaaacgg ttactcgacc ttgagatgga gaatggacag ctgaaacggc
    10441 aggtagcgga acttcttctg gactacaatg cgttgagagt ggcgcttgtg aatgaagcca
    10501 gctccgcatg ctagacgtta ttcacagaag ccgttgctct tgcgaataaa gaaggctcat
    10561 ggtcggaatt cgtgaagaca gccagtatga tcaggtattt ctcccgcata tgtgctcggg
    10621 cgttctttgt tctcgctcta attcttggcg cggtcctgct cattgctagt gaaggagagt
    10681 gtcaacagat cagtgccatt actccaccca ctgctgggac ttctttgctc ggcgggcagg
    10741 gatacatgtg gttatcccag tttgccaatc cgggagtctt gggcgcgctc tatcagactc
    10801 ccactttccc atggacaggt atctatcccc tctacccggg cagttttaca gcgatcgctc
    10861 ccgacgacgg atttcgagca gggccattgc gattgcatcc cttcatgggc atggcggaaa
    10921 tgtttaccga caatgtcctt cgcaccaaca gtaaacgcag cgatttcttc acgaccttgg
    10981 cgcctggcct gcaagcgcaa ttgccatttg gaactcggca cctattcgta gcggactacc
    11041 gcactaacct tcaatttttt caccgtaccc catcaaacaa tgtccaggat cagacggcgt
    11101 ccgccatgac gcgttttgag tttgcaagcg gcctgaagct cgatctccag ggcgaacaca
    11161 agctcgggca cgaccctcgg gggtccgcac tagatcttca gactttagaa ttgaacaaat
    11221 gggtagcgaa tagtattact ggtcgcgccc aatacgaggg cggtcaagtc ggggccacat
    11281 tgaccatgca gacaatgcga tggaattata tcaacaacaa ccagggcctt attcgggatc
    11341 gtcttaccaa ctatcttggt acgacttttt ctgccaaggt gctgcctcac acttccgcct
    11401 tacttaacct cagcgtcgtc caggaaattt atgatgagaa taaaaacctg gatagcgcca
    11461 tgtatacaat aagcactgga gcccgatggg aaattagcgg taacacgtcc gggcagttcc
    11521 tggtgggata ccagcacctc aagttcactc gcgcccaagt caaccaaccg ggaccggttt
    11581 taagtatttt tcggcgagat caggattcgt ttaacaacct ttttgcgatg gggcaacttc
    11641 aatggacgcc gctggccaac ctgaaccttg cgctgcaacc atatcgcacc attcagcaga
    11701 ccgtcgtggc tggcaccatc ttctttaccg ccacaggcgt caatctgtca gccacatacc
    11761 cactaggcat gcggtttgaa gtgaccagta atcttggatt tgaagtcgac cggttctcga
    11821 gcccggccgg agcacttgcc ttgacaccga ctcgttccga cacgctgaag aacgccgcca
    11881 tcgggctgaa ttatcgtgct gtccggtggc tgggcgtaag cctccagtat gtgttcgaag
    11941 atcggagttc aaacgccagg gatttcaact accaggcgaa taccgcgatg ctttcggttc
    12001 aaggaatgtt ttaaccgcaa acaaatgctc gatggaccaa acctctgcgt tcaagtgttc
    12061 cagctgcgga tcacaaaagg ggagagaggt gaagagcaag ttggaggcgt ctaacgcacg
    12121 gtaacaccaa agggctgcga gggtggtgtc gtatctaccc cccccacctg gtacgcgatc
    12181 actgcctccg cattgcgaat ctttacgatg gtcgtgggag ccgaaagcgt cccattttct
    12241 agtaaagttt gaatcggggt ggtaaacgtc accgtagctg cgctcgcatc gctaaagtcc
    12301 gtgcaggttg ggccgtcttt cggacgtacg agtaccaggg ctttcgtgaa agaccggccg
    12361 aagatctgat acgaacagcc tgcagttccc tgctgataca gaatggtatc tcctgacggc
    12421 tgaccgacat cgacttgata ggcgaggagc cattctgact gatccttact tgactgatca
    12481 atggttggag tttcgtcaga acagaaacca gggttgaaat agacagttcc gggactgccc
    12541 gcaggctcct tgaattggta ataacttgcc agtcgccaca ttcgatatcg tgcggcggaa
    12601 gagccatagt tgccggagga atattcggcg gggcccgtat agcaccagat gccatggagg
    12661 tctaccagtc ccccggctgt cgtaagaccc ttgatgagag ttaggtactg ctgatactcc
    12721 gcagggcctg aaaacccgtc gggcctatgt ttactttccg tgcttattcc gtggattgca
    12781 tttgcctgac ctagaagcca ggggtgaagg gcattttcat tcgcattaat cagcaccttc
    12841 tttgacactt gagcggcttg gattgccaaa aagctcaacc aatctgtgac tgcattggca
    12901 tactcagatt cgaagcttga attgcccggt cttcgcccgg cgaattcacg gatgcctccc
    12961 cccgattgca caattgtttg atagccaatg ctgagtggcc atgaaaaacc aggagcatgt
    13021 tcgtcaatcc aaataaagcg gctcgtgtcg gtggcatatc catacccatt caacggccct
    13081 aatatttttg cagctttcca agcacggaat gccgtgcttt gcggattgaa tacgtagcgc
    13141 cggtcattcc acaagtgagt ctgtactcgg cagcccgaag acaagggagt gttttctgga
    13201 catccggcga ccgttgccgt gccaactact tgcttccggt ccagactata aaacgtcaga
    13261 acagtactct cagaaaaatg cagaaacgct tcttccggta ctccaaccag agccgccgac
    13321 gtatatgtgg ataggtcgaa ctgatagctg aaaagctcac ttgtaggatt taaggtccga
    13381 acggccggac cgtttccttg cacttcaact gcatccaggt gtgaaacgta ccattgatat
    13441 gtgtcattgg aaagattggc gagaaacagc gaacgggcat aggcgaaaag attaatgtgg
    13501 ggccaatgag gactctgtgg agctggaggt tgagcaagac tttgcgcatg gaggggctta
    13561 ccgatgccaa aagtacaccc aatcatcaaa aggcctacta gtacaatctg ccgggaagct
    13621 ttcatcatga tctgcctttc tcaggctgtt cctcacctct aattcttggc agagggtttc
    13681 aaattaagcc ttgttcggta acgagcatag aatgtgccag gagatcattc gttagcaact
    13741 gtctggtatt gaggctcaaa ttttcaaggc tcgccgatca accgtattca agaccatacg
    13801 atgccggacc aaaaccgatc aagaatcatc tgtcggtcga ttatagctgc gcgcactcca
    13861 gcaccacttc attctgaagg acgatatgaa tactcgcaaa gtagatcagg aatcctattt
    13921 aatcgacctt ttgagggttc tgaagcaacg atggtggata attattctct gctcgatgtg
    13981 ctgcgcagca atcatggccc tatggtctca tgtccaggcc ccaggatttg agtcaacgac
    14041 aaccgtgcta atcgagcagg agcatcaggg accactgcag aaatcagata ggaccatggc
    14101 cgacttctct cccgactatt atgaaacgca tttcgagctc ctgaaaagtc gccgcgtcct
    14161 tgaaaaagct gcggaagagc tcaagcttct tgaacggtca gaatatttaa ctttcgccgg
    14221 attgtatgaa aacttgctca tcactgtaca gaccacgttt ggttcgaaga ccaccacaac
    14281 ttcttcgact ccacccccac tcgcaagcgg ggaacgaacg aacgagtcat gggaccttgc
    14341 gctgggtcga tttgagcaga cgatccagat taagccggtg cggggaacca ggctggcaaa
    14401 aattaccgtc aaatccgggg atgctcactt tgctgcggag gcagcaaatg ccatcgcgag
    14461 agcatacata cacctcaacg aagaggtcca tgcccagaag aaagaaaggg cacgagaatg
    14521 gttctcctct cacttagcgg atctgagaaa taaagttgag caatcacaag aagccctgaa
    14581 tgcataccga gtgaagtatg gggtcgtcga aacgggggac cgtcagacac catccaggca
    14641 gcgcctggca gagttgagtt cggaactcgt caaattggaa cttcgacgtg cagaagcgga
    14701 atcgcgatac caacagatcg cgcttctcat gccagagaag acgaaagccg aaggaatcga
    14761 ttgggcgaaa tggggtgatc acgccgaagt cctctcttct ccacttattc aagttcttcg
    14821 cactcaggag attaaggcat ccagcgaggt ggcggagcta gctgaaaaat atggttcgct
    14881 gcatcctaaa atagcccgtg cggaagccga gcttcatcaa ctgagagagc gggttgtaga
    14941 agaggtacgg aaaatatatg cctcgattag gcatgagcga gatctcgcca cgacgagaga
    15001 atcagcaatc cgtgtagcag ttgatcgaca gaagcgtgat gtaatgcagt ttgatcaata
    15061 tcaggctcaa cagggtattc ttgaacggga ggtcaagagt gctcaacacg tgtacgatct
    15121 ttttctggga gaggcaaagg aagcgtcgtt agcctccgaa cttgtcgcta cgaatgtggt
    15181 gattgcggat cccgccacgc ccgcgtcgaa gccaagcaga ccaaggaaag ttgccaatac
    15241 cattcttggt ctgctgagtg gactggtatt cggccttgga atcgcaatcg gtctagatca
    15301 ttttgatcaa agcctcaaag gccctcgtga tttggccagc tatctaccca ccattgcttt
    15361 tcttggcgcc attccgcgtg taaagcacct cgaaacgcag gagccgatga tcctccgggg
    15421 cccgcgggct ctgccggccc ttgaagcgtt ccggtcgatt cgcacaagca ttatgctctc
    15481 tggaatgata ccggaccacg gttctctggt catcacgagt tccggtccgg acgaagggaa
    15541 aagtacagtg gcggtgaatc ttgcgtttgc catggctcag attgaagatg gacgcgtgtt
    15601 gctcatcgat gctgatctga ggagctcaca tccaggcagt ctattccttc tcaaaggcga
    15661 tggtatccga gggcttgggg acttcttaga tggcgatgcg gagttcgatg acgtggtcca
    15721 tcacagtggt gaatccaatc tttggacgat tcctccgggc aaagttacaa cgaatccctc
    15781 tgagttgctc tcctccagac ggatgaaact cctgctggaa aagtgtcgca ccgcggggta
    15841 cttcgtcatc atggatacgc ctccggtcac actgttcagt gacgcggtga ttctcggcag
    15901 tcgggcagac ggagtcatgc taattgtcgg tgcgggacag gccgaccgag aagcctgtca
    15961 attggctggc caccggattg tccaggctgg agggagactc ttgggagtgg tcctccaaaa
    16021 ggccatgggc cctcggctat cgcactattc tcaatacgct cagattcagt aagccgatgc
    16081 gcgattccac taaccgcccg ggagagtttc ggtaatgagc caggcagact tgaatccggc
    16141 gtcagtcccg atggccccca tagagacggg catcaggttc tcgcatggcg tggcgagcga
    16201 gatcgcctcg ccagcaccct ccatcagacg gtcggccctg tggaccggtc ttggtgatgc
    16261 gttctacgcg ctgtgccaat gggggattct tgtcatactc gcgaggatgg ggacaccgga
    16321 gatggttggg cagttcgcgt tagcatctgc gattacggct ccggtgatca tttttgcaaa
    16381 cctggggctt cggcagctgc aatcgacaga tgcacggaat caatttcagt ttagggacta
    16441 cgtggccatg cgactcgcga gcacggtcct tgccctcgca acgatcgccg tgttggcatt
    16501 ccagtatcgg gtagatactg cgctcacgat tcttgtgctc ggtatcgcaa agtgctttga
    16561 agccattagt gatgtgttct acgggctact ccaaaaacat gagcgtatgg atttgattac
    16621 catttcgctc cttctgagag gtgtgacgtc ggcagctggc ttcgccgttg gctttattct
    16681 tctcgggggg gtactgggcg gggtagtcgg tttggcaatg ggtgggcggc ttgttttgat
    16741 tgcgtatgac attccgaggg gcagccagat tttgtcattg acggcgcata agccccatac
    16801 agcagggatt tggcggcaga gccagatgca ggcactgtgc gcgttggcct tgccactagg
    16861 agtggggacg atgttgctct cgctcaatgc caatatacct cggtatttta tagaacgata
    16921 cataggcgat ggtgccctgg ggctatttgc gggaatggca tacgtggctg cggccgctgg
    16981 aatggctgtg aactccctgg gccaagccat gagtccgcga ctctcacagc attgggctag
    17041 aggggcgttt gtggagtttc gcacgttgtt tatgaaattt gcagctctgg cagggctgtg
    17101 gggtcttatt ggcatcggtg tggcgtttgc cgttggcgcg cagctgctca cacttcttta
    17161 tggggcggac tatgcgactc actccgacac atttgtctgg ctcatggggg tggcggcggt
    17221 atctcacctt gccagtgtct gtggatttgg catgacggcg gcacggatta tccgtagtca
    17281 aagcattcag tttgcctgcg tagcagccat cggaaccgtc agtgccatag ttctcattcc
    17341 cggccatggc ctcaatgctg cagcattagc ctttgggatt tctcttttcg cacagctatt
    17401 cataagcgca ttgtgcctcc attacgctct cgctcggaaa gaccaaccct gcaagagtta
    17461 gccggtcggt ttttaattga gggtgagcgg tctccatgtc taccgcctca
//
LOCUS       DAORQN010000022        16829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_226_length_16829_cov_165.858770, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000022 DAORQN010000000
VERSION     DAORQN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226_length_16829_cov_165.858770"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PK614_02860"
     CDS             <1..471
                     /locus_tag="PK614_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB81179.1"
                     /translation="RQLPQVSDYVFPGQDLSKPWNESTQRKAWDRVRSVANLPDVIAY
                     DLRRTMASWMAQHGLNLSVIQQSLNHSTLQHTSRYARLDVQTAGRALDGVSKHMAASN
                     RFFNDLCAPLTSGELLPEVQALNEAEEAPQSYDTQARSSADPSETDPPDMEFPG"
     gene            560..1057
                     /locus_tag="PK614_02865"
     CDS             560..1057
                     /locus_tag="PK614_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81180.1"
                     /translation="MKTSGAVLLSGVLLLQGCSGLIIREQDSVGMKAAKVAARVLAWP
                     VTLTLSEVEIATIKQREELEASQQQAQLSQRNHFEGLVGRLTYSEALVRWGPPVQSQT
                     HGGMIVAVWQSRRASPGYLLMPPIPGNPYSSSLAVALPESGSRLQLVFDQETERLRSW
                     SVQDW"
     gene            complement(1705..2010)
                     /locus_tag="PK614_02870"
     CDS             complement(1705..2010)
                     /locus_tag="PK614_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81181.1"
                     /translation="MPTTTLQSKPRRYRGDNYWEKEAPLKVTTDKVRICFYPRAQKLQ
                     LSAGWKNRNTGKVRYGRTVVLDSASLTASPEALALFETVLATAPRRLPLAPEPTGTI"
     gene            complement(2087..2602)
                     /locus_tag="PK614_02875"
     CDS             complement(2087..2602)
                     /locus_tag="PK614_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81182.1"
                     /translation="MNRLAPYAALLAFCLTTCVPVLAEEFAAPLEEADKILAPFSKEV
                     TDCVKKVTQWLPPVEIQTLELALDKVSIIPGHEASSLRIPLRHARGPMIELHQALKDL
                     DGALQFLRLLQTGKIGSVTAEAYDSLFITDRLARRFSKQDVRTCERSHVLTVIKECTV
                     CTITRWNSVKP"
     gene            2759..3361
                     /locus_tag="PK614_02880"
     CDS             2759..3361
                     /locus_tag="PK614_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81183.1"
                     /translation="MPTDSIPLNALLIKLEGLRKEQKWTPETLRGLIGFIRRADDQED
                     KAESNKDLARAHDLMGKAVAAYKLIKSVPPDLASRLTALQYELMGQKPPELSWSQWRT
                     WNLAPFFSGPSKWHACEFFLQKVVAETQRASAGAMKTEHSRMKRKYGAKHDSLPLDSQ
                     VALLLDRKNTRHNERSRIQIITNRSAHPRLQLALRRNSRG"
     gene            4016..5002
                     /locus_tag="PK614_02885"
     CDS             4016..5002
                     /locus_tag="PK614_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81184.1"
                     /translation="MKARPSFQFYPSDWLRDPGLRSCSLAARGLWIDSISFMHEGEPY
                     GHLTLNGHDIEPATLARMVGSPLRDVRRALVELETAGVFSRTEKGTIFSRRMVRDEAL
                     REKRAEGGHLSQNHPDVPKKKDRAKDTIKDTLPPSFNGSIEGSPSSSSSSSSSSSGKK
                     EEEKMTGATPSTPIDPPQGSDEHDALTPLEIHSMWNSIPGVKTCRELGKGIRTRLQSR
                     IKEHPERVWWDGLFAQVRVSNFLCGRTNNSRGPFQASLSWALSPENLEKILAGNYDNP
                     EPVDGQPSPGCEFRVSNGRFNKACGKNRVHGSKYCEQHRSSRSVLPANEVTL"
     gene            5002..6333
                     /gene="dnaB"
                     /locus_tag="PK614_02890"
     CDS             5002..6333
                     /gene="dnaB"
                     /locus_tag="PK614_02890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRB81185.1"
                     /translation="MSSTLSTLPPQDLEAEQSVLGAILLDADILSTVTGVLTQRDFYK
                     TTHGVIYAAMETLADGGEPVDHLTLSDQLRKTGQLEGVGGSSYLAELLSCVASTSNVK
                     HHCQLVREKSLRRQLGHTGLEIHRKAYDEQEPLADLLDSTEAQIFSLGQGRVSTSFEP
                     MRELLKDSLQDLDRLYKRRSSVTGVPTGFATLDRWTAGWQAGDLIIIGARPSMGKTSL
                     ALGMAVHAALQEQRVVGIFSLEMSKRQLVYRILSAEASIDAHVLRTGQLSKDSFWRLA
                     EAAGRLENIALSIDDSGGLTVSQLRGKARRLKAERGLDLLVVDYLQLMQGRSDSESRQ
                     QEISDISRSLKSLAKELDVPVIALSQLSRAVETRKPPIPMLADLRESGAIEQDADVVM
                     FIYREDFYDPESPRKGIAEILIRKHRNGPTGDIELEFSEQYARFREISQHE"
     gene            6326..6730
                     /locus_tag="PK614_02895"
     CDS             6326..6730
                     /locus_tag="PK614_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81186.1"
                     /translation="MSSTRRELPNRRNHHTQKVRIAGQRTAYVSVHEDQAPAELFIRV
                     RGIDCTSEVVGLYDIVGRLASLALQYGAPLEKVGAMLLGSKFELAGPVTLHPRIKNCT
                     SLPDLIGRHLLVKFCNRADLAHVSEENNEGRN"
     gene            6733..7461
                     /locus_tag="PK614_02900"
     CDS             6733..7461
                     /locus_tag="PK614_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81187.1"
                     /translation="MPAMMMALGGGALSVGGGIAKAFGGDSQAKAARKAAQMYIDYQE
                     RERKKFLDANSPIRDKLMQYSQGNSGYDADLVRGMKDDVNEDYGQGLADVSRISANSA
                     VGKNSGGGNVYTPGRYDRATRQLGQNLAMNKAKMIRDVNQKNADMAISNQRFAVSALP
                     TYSPGLSATPQIGYDAFQGLNAVAPVGNYIGDMLQSVGQPAMSAGMDGLVRGPIAMKL
                     AQMQRQQQQPGPWENFYTSPHLQY"
     gene            7750..8025
                     /locus_tag="PK614_02905"
     CDS             7750..8025
                     /locus_tag="PK614_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81188.1"
                     /translation="MSNLPLQRAVSPGYLDLRALAVYSSSSVRWLRDRINDTTAPLPY
                     HRVGGKILIRKEDFDDWISRFRTIQSATELETLVDGVMADLLTNKSS"
     gene            8265..9731
                     /locus_tag="PK614_02910"
     CDS             8265..9731
                     /locus_tag="PK614_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB81189.1"
                     /translation="MAVKVRERPAGSGKWWIYTDFRGKRTARYIPQGKRAAEKVASEI
                     AAKLDLIKQTERHGLTVSPREVITGRKDLSPVKTAPAVIGPLFSDYAAQWLDACEARG
                     LKASTYRSYKTILSVHLLPAFGPLHLSEIDRRAVKLFVQTKLTEELAVGRKEAQNKKP
                     RAPRTVLHFLRALSTLFNSAIEDHYPVVNPALNPGKLLRTKRTSEDINPFDRDEEATF
                     LQAVQDRTPRYYPFFLTLLRTGCRLGEAIALQAGDLDFRGKFIEIRRNFTNGNLTTPK
                     SNKPRRVDMSDKLIEVLRDHVVSQELEAMGRNQPKPEWLFTNEGGQRLDPDNVRKRVF
                     YRLLEKAQIRRIRIHDLRHTYATRLVSNNESLAYVRDQMGHSSIQVTVDLYSHYVPGS
                     NRQAVNRLDERVEKREPESKSATSRNQESAEESESPKSLISGAGEWNRTTDLRFTNQA
                     RTIFETLGVSSGFPVLTANTGTCTHSTDSMDSRGISAF"
     gene            9854..10579
                     /locus_tag="PK614_02915"
     CDS             9854..10579
                     /locus_tag="PK614_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81190.1"
                     /translation="MISSFVPVSVLEAKRLQNVFNESSLFLHPFKDPLLIDFGAHRWL
                     SSAREESYSDWLAWILTQLQRPDLVFGVLLEKYDANLQEKCKQRLTVHRELGLVDEDG
                     FRKRTDLELMYGAEKAVLVEVKKGDASHIDLEQLRVQLRHKPGFGHYVLLARHGEHRQ
                     YADGFQLRTWNDVCVALRRIVAIPELKGTVEGAMILAFVGAVEQNLLGYPGNFSERFA
                     RGELISVSLRQRIEAHLKSTING"
     gene            10572..11156
                     /locus_tag="PK614_02920"
     CDS             10572..11156
                     /locus_tag="PK614_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81191.1"
                     /translation="MVDHLERAESYCHFINEGLRSYLDARIAIGAFSDLILNECASSM
                     QKFLPSLSEVLGVNLQQKDIGYSTEPSGANNSPSDWNQNFICLQAKIDLSKKRNDLYQ
                     LATGLCWDKNDGPVSIWPYTCLVTQTLEKANALYKTLGNDEIDNTENYCLTIYGDSPS
                     LKDGFPYAELEWTLSEWISVLRKTKALLNKTAKG"
     gene            11280..12422
                     /locus_tag="PK614_02925"
     CDS             11280..12422
                     /locus_tag="PK614_02925"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRB81192.1"
                     /translation="MSLDLQLLSSKLTRYRKQFDSSIDEVAAATGISIEALSDYEQGR
                     SEPTGDEILILADYYKCDYKFFISNEQLAAFEQTETLFRSYSNELSKEDRWAIQEFLF
                     LCGCEEFLQREFSHKRTPFVFQKTGKHYKAHAVSAALNLRRYLQYKTTEVGRDIYKDF
                     RSIGFHVFRRHLSNSAISGLCIRHPFAGKCILVNFTQDIYRQRFTAAHEAGHAILDDD
                     RDTVISFTWDKKDLSEIRANTFASRYLMPPEFLTLLPKNAKWNDTAVVKWATNLMVST
                     EALAYALKDAGIVSQEEADVIRAVRIPRQAKTDPELPESLPPRARERKEEFMQRGLSD
                     FYVNLCFKAYDQDLISAGRLGEMLLIQDHELASIADLYGQPLKHGD"
     gene            12412..13002
                     /locus_tag="PK614_02930"
     CDS             12412..13002
                     /locus_tag="PK614_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81193.1"
                     /translation="MGIDVSRFHPHNVTDTCAVWHVLSSQILYATALSMGCHFCITQF
                     VLYECLFKPRTHSLIGRELQSRLRREQSKGIFKSYPLAVEDLQDIGILENRQRLSKGE
                     LSSIAFAKKTSQAFLTDDQGARKLARGVVTQGVQTTPHLFGWLFFSGHLADGDRTALI
                     REHEAMGGRLSRYFEEMYLESLRCRLMASHSKTADK"
     gene            complement(13045..14001)
                     /locus_tag="PK614_02935"
     CDS             complement(13045..14001)
                     /locus_tag="PK614_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81194.1"
                     /translation="MALEMVLNELSLAKAANLFEARAWMSELIKTSSFAASHGVARVL
                     HSEPNVHSLNIADGYPLARWRNDEDVPREERQYFRSLITKSPLVMVELEDGEGESSLP
                     PEYLFDNQAAKGLGFAFEGNRVAISLQSKPDWNKSILEIIVQSLSADGDLEISNGKVH
                     HASHRSHITENLGWIKRYTTSKLSSGRDLVRRRGELLPSLEFCSTALEQLEGLTQSSP
                     HFIQIVKHLLAFQEYCENWTAEMFVSSSFPGKITKESGPTLQLYGAERTFVCPDGRQR
                     LFVWHARITPEAWRIYFEPQVERRALTIAYIGPHLSTVRYPT"
     gene            complement(14008..15117)
                     /locus_tag="PK614_02940"
     CDS             complement(14008..15117)
                     /locus_tag="PK614_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HRB81195.1"
                     /translation="MISLLHIHNFKCFESQRFDFGALTLLAGLNGMGKSSLLQSLLLL
                     RQSHSQGLLDTRGLALNGDLVTVGNASDALYENAKEEYIGYDLHFSDGAKARWRFDYN
                     READVLSVTPDSVSLQSYAQGLFSNNFHYLQAERVGPRTSFPMSDFIVRNTRQIGVKG
                     EYCSHFLSVFGRSDIPGQARRHLRAESFKLTDQIEAWMGEFSPGLRTHFTTHPGMDLV
                     NLEYSFVSGKQLSNRYRSTNVGFGLTYTLPVLTVLLSAGPGGLVLLENPEAHLHPRGQ
                     GKLGELIVRAALDGVQVILETHSDHVLNGIRVAIRRLQFDPEKVMMYFFSCAEVDGEF
                     VRRISPIRVDSEGRISPWPDGFFDEWDKSLEGLLE"
     gene            complement(15114..16229)
                     /locus_tag="PK614_02945"
     CDS             complement(15114..16229)
                     /locus_tag="PK614_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRB81196.1"
                     /translation="MELFTSSESDENGVELEEVNDFITKPFDPTLIRVESKTLSVDLL
                     LTRIKEGEINLSPSFQRMADIWSPTAQSRLVESLLIRIPLPAFYMDATDENSWLVVDG
                     LQRLTALKKFVIDKQLALIGLEFLTQLNGARFDELPRFLKRRINETQITVFLIEKGTP
                     PDVKFNIFKRINTGGLPLSPQEIRHALNQGQVTTLLEELASSEEFLAATDRGINSKRM
                     VDRECVLRFIAFTLTPPTDYKTADFDSFLNAAMIEINHAPRHRIERLTSQFCNAMKAA
                     HHIFDRQAFRKMSAGRTGRSLINKALFETWSVNLAALSEDEVARLVARKGIVLEKFCA
                     LLLERDFEASISQGTGDPSRVRTRFGSIQKLIQQSLL"
     gene            16458..16706
                     /locus_tag="PK614_02950"
     CDS             16458..16706
                     /locus_tag="PK614_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81197.1"
                     /translation="MKSDDAKKETLQFKVTGQERKLIEKCAEEEGTTVSKYVRGAVLM
                     SLVMDGKAEAIKIVAREVGEKAFGVVRQKLVRPTQEGR"
BASE COUNT         4169 a         4268 c         4413 g         3979 t
ORIGIN      
        1 cgccagctac ctcaggtttc cgactatgtg tttccgggtc aggacctttc gaagccctgg
       61 aatgaaagca cgcagcgtaa ggcctgggac agagtccgat cggtcgccaa cctgcccgat
      121 gtgatcgcct atgatctccg gcggaccatg gcgagctgga tggcgcaaca tgggctaaat
      181 ctttccgtta tccaacagag cctgaaccac agcacgctac aacatacctc tcgttatgcc
      241 cgtcttgacg tccagaccgc gggccgtgcc ttggatggcg tctctaagca catggctgcc
      301 tcgaatcgat tctttaacga cctctgcgcg cctctcacat ctggggaact gcttccggag
      361 gtgcaggcgt tgaatgaagc ggaagaggcg ccgcaatcct atgacaccca agcgcggtcc
      421 tcggccgatc catccgaaac ggatccgccg gacatggagt tccctgggta gcacggcagt
      481 ggatacgggt gaggagggac ggttgacctc ctcgctcctt catccggacc ggaataatca
      541 aacggaggag gagctcacca tgaaaacttc aggggcggta ctgctgagcg gcgtgctgtt
      601 actgcagggc tgctcggggt tgatcattcg ggagcaggat tcagtcggaa tgaaagcggc
      661 caaggtggca gcgcgggtcc tggcatggcc ggtgacgttg accttgagcg aggtggagat
      721 agcgaccatc aagcagcggg aggagttgga ggcaagtcaa caacaggctc agctgagcca
      781 acggaaccat ttcgagggac ttgtcggccg gctgacctat tcagaggcgc tcgtgaggtg
      841 gggccctccg gtacagtcac agacacatgg tgggatgatt gttgccgtgt ggcagagccg
      901 ccgggcaagt cctggctatc tgctcatgcc gccgatccct ggtaatccct attcctcctc
      961 cctggcagtg gcgttgcctg aatcgggctc gcgtcttcag ctggtctttg atcaggagac
     1021 ggagcgcctt aggtcatgga gtgtgcagga ctggtagcgc aagcagcgaa aggcatcacg
     1081 cattagggcc tcggatcggc aacggtccgg gctttttatt ggcccctgaa acggtgctta
     1141 ccgctccgtc gcctttcccc tttgatccac caaccaataa ctcttattgg cagacgaacg
     1201 tattggccct aaaaaccctt taggatctgg tcccttcagg ttacccttgg tccggcctgc
     1261 tttagatcgc tccgccttga atccgttcga aggctggggg tgccgaggaa ggctcatagg
     1321 tcccattcct cacatcagcc gaatcaataa cactcctgcc aataacttct gctgactctg
     1381 aggaaggaaa ccaacggttc tggatgagtt cagatcgggg atgaggtcgg cgggtgagtg
     1441 ggggaaggtg cgcctttccc ggagtcggac tctcggagtt tcggagagag caaagagggt
     1501 aacgagaaaa cagggggatg gggggtaggt gggtcatacc ttcctccctc tgactgactt
     1561 cctaaaaacc gcggtaccac cgctttcttg gttccggttg catatatggg cagatgtcca
     1621 ttcgcgtggt acctgaccaa tagaggcgtt tctggctatt ccgagggccc gtgacgcgcg
     1681 agatcagcac gtgtattctg gacttcagat ggttccggtg ggttctggtg cgaggggcag
     1741 cctacggggg gcagtggcta gaaccgtttc gaataaggcg agggcttcag gactggctgt
     1801 aagacttgcg ctgtcgagaa ctaccgtgcg gccgtatctg acttttccag tgttacggtt
     1861 cttccagccg gcagatagct gaagtttctg agcacgcgga taaaagcaga tgcgaacctt
     1921 gtcggtcgtc accttaagtg gcgcctcttt ttcccagtag ttgtcgccgc gataacgacg
     1981 aggctttgat tgcagggtgg tggtgggcat gaggagactt taagccgagc aagaattagg
     2041 gtcaagagca cagcgctacg agcgatctac cgacgagtcc tcgcctttac ggcttgacgc
     2101 tgttccatct ggtaattgtg cagacggtgc attctttgat gactgtgagg acatggcttc
     2161 tttcgcacgt gcgaacgtcc tgcttcgaaa acctccttgc gaggcgatcg gtgatgaaaa
     2221 ggctgtcgta agcttcagcc gtaacactac ctatcttgcc cgtttgcaag agccgcagga
     2281 actgcaaggc cccatccaga tctttcaggg cttggtgaag ttcgatcatg gggcctcgcg
     2341 cgtgcctcaa aggaattcgc aagctggatg cctcgtgccc aggaataatt gaaactttat
     2401 ctagcgcgag ctctaaagtt tgaatctcga ccggaggaag ccattgggta accttcttta
     2461 cacagtcggt tacttccttg ctgaaaggcg ccaagatctt atcggcttcc tccagtggcg
     2521 ccgcaaactc ttcggcgaga acgggaacgc aggtagtaag gcagaacgcc agtaaggctg
     2581 cgtagggagc aaggcgattc atttgtagac ctcagaggtt gttgcctacg tgctgaaatt
     2641 agttcgccag tgtagaatag gttctcacgg gcagcaacgg tcgggcttga tcacctgacc
     2701 ggccagcgtt cctggcgctg gcgctgccct tcttcaccag gacccgacag gagggggcat
     2761 gcccacggat tctattccgc ttaatgcgct tttaattaaa cttgagggcc tccgcaaaga
     2821 gcaaaaatgg acgccagaaa cccttcgagg gttgatcggt tttatacgcc gtgcggatga
     2881 tcaggaagat aaggccgaat caaataaaga tctggctcgt gcgcatgatt taatgggtaa
     2941 ggcggttgcg gcgtacaagc tgatcaaatc ggttccgccg gatcttgcgt ccagactcac
     3001 cgcgcttcaa tacgaattga tgggtcaaaa acctcctgaa ctttcttggt cacagtggcg
     3061 aacatggaac cttgcgccct tcttcagtgg ccctagcaaa tggcacgcct gtgaattctt
     3121 ccttcaaaag gttgttgcgg agacgcaacg ggcgagcgct ggggccatga agacagaaca
     3181 ttcgcgaatg aagcgcaagt atggtgccaa gcatgattcc ctgccactgg attcacaggt
     3241 tgctcttttg ctcgatcgta agaacactcg tcataatgaa cgatcccgga tccaaatcat
     3301 caccaaccgc tctgcgcacc ctcgactgca actagctctc cgcagaaaca gccgcggcta
     3361 aatcaagcga ttacacgtcc tcgtacgcct gacagtggtg ggagaatacc caacaccgga
     3421 gcgggtgttc ggcgttctac ggccttgcgc cgttacaaat agatgctcgc atttgtacca
     3481 tcaattcaaa tgaatccttc cgtgctctca tggttttatg gaccatgacg catccacaac
     3541 aaaaggggca cgaaatgact tccattctgg ctacggtcga tgttgctcga attctcgggg
     3601 tcacatgcgc atcagtgcgt gcatatgaga cgaagggaca gctgcgcgca aatcgcacac
     3661 caagtgggat tcgggtattc agcgagtcgg aagtcttgcg cttcaaacag gaacgaagcg
     3721 ctagaaaccg tttccggaaa tcggccgcca gttcgggggc taagctcggc gcagtgtcca
     3781 aggatcaata aaaaccgcca ggggtggcag cccgaggcgg ttcaatcgaa ggagcatgtg
     3841 agatgcacag cgtacttcat tcaaataggc cgcagcaacc agcgggagtt tctcaggcaa
     3901 accgaactgc gtattgggct gaagagatcg ctcgccggct tcctccactt caagccctgg
     3961 cgttccggaa atactcgaag aagcgtctcc ggtcgattcg gggaggaagg cggtaatgaa
     4021 agcccgtccg agtttccagt tttacccttc cgattggtta cgagatccag gattgcgatc
     4081 atgctcgctt gccgccagag gattgtggat cgactcgatc agctttatgc acgagggtga
     4141 gccctacggc cacctgacct tgaatggcca tgacatcgaa ccggctacgt tggcgcgtat
     4201 ggtcggctca ccgctccgag atgtgcgacg tgccttagtc gaactcgaaa cggctggcgt
     4261 gttcagtcgt accgagaagg ggaccatctt tagccggcgc atggtgcgcg atgaggctct
     4321 tcgcgaaaaa cgggcggaag ggggccacct gtcccagaat catcccgatg tcccaaagaa
     4381 gaaggatagg gcgaaggata cgatcaagga taccttgcca ccatccttta acgggtccat
     4441 agaggggtcc ccttcttctt cctcttcttc ttcctcttca tcttcaggga aaaaagagga
     4501 agagaagatg accggcgcga ctccgtcgac gcctatcgat cctcctcagg gtagtgatga
     4561 acatgacgcg ctcacgcctc tggaaattca ctccatgtgg aactcgatcc caggagtgaa
     4621 gacctgccgt gaacttggga agggaatccg gacgcgcctc cagtctcgaa tcaaggaaca
     4681 tcctgagcgc gtatggtggg acggtctctt tgctcaggtt cgcgtgtcca attttctctg
     4741 tggtcgtacc aacaactccc gaggcccgtt tcaagcatct ctctcgtggg cgttgagccc
     4801 ggagaatctc gaaaagattc ttgccggcaa ctacgacaat ccggaaccgg tggacggcca
     4861 gccatctcca ggttgtgagt tccgggtgtc gaatggccga tttaataagg cttgcgggaa
     4921 aaatcgcgtc catggttcga agtattgcga gcaacatcgc tcatcgaggt ctgttcttcc
     4981 agcaaacgag gtgactctgt gatgtcctca actctttcca cgttaccccc gcaagacctt
     5041 gaagcggaac agtctgtgct tggcgcgatc ctgctggatg ccgacattct ttcgacggtt
     5101 accggtgtcc tcacccagcg agatttctat aagactactc atggtgtgat ctatgccgct
     5161 atggagacac tggcagatgg aggcgaaccg gttgaccacc tgactctgtc ggatcagctc
     5221 cggaagaccg gacagcttga gggcgttggt ggatcttcct atcttgccga gctgctcagc
     5281 tgtgtagcgt ccacttctaa cgtcaaacat cactgtcagt tggtccggga gaagtctctc
     5341 aggaggcaac tcggacacac agggcttgag attcatcgga aagcgtatga cgagcaagag
     5401 ccccttgcgg atttgctgga cagtaccgaa gcgcagattt ttagcctcgg acaaggacgg
     5461 gtgagcacct cgtttgaacc gatgagggag ttgctcaagg atagcctgca agatctcgat
     5521 cgtctgtaca aacgcagaag ctctgtcacc ggagttccta cggggtttgc cacactcgat
     5581 cgatggactg ctggttggca agccggcgat ttgatcatca tcggcgcgcg accaagtatg
     5641 ggaaaaacga gcttggcgct tgggatggcc gtccatgcgg cgctacagga acagcgggtc
     5701 gtaggcatct tcagtcttga gatgtcgaag cggcaactcg tctatcgaat cctcagcgca
     5761 gaggcctcga ttgatgccca tgtgctacgg acaggacaac tcagcaaaga tagcttttgg
     5821 cgattggctg aagcagcagg gcggcttgag aatatagcgc tctcgatcga cgacagcggc
     5881 ggcctgacgg tgtctcaact ccgtgggaaa gcccggcgcc ttaaggccga gcgtggcctt
     5941 gatttgttgg tcgtggacta tctgcaactc atgcaggggc ggagcgattc cgaatcgagg
     6001 cagcaggaga tttcggacat ctcacgttcg cttaagagtc tcgcgaagga actcgatgtg
     6061 ccagtgattg cactctctca actaagccgg gccgtcgaga ccaggaagcc tcccattcct
     6121 atgctggcag atttgcgaga aagcggggcc attgagcagg acgccgacgt ggtgatgttc
     6181 atctatcgag aagacttcta tgacccggag tcgccgagaa agggtatcgc tgaaatcttg
     6241 attcggaagc atcgcaacgg accaaccgga gatattgagc tcgaattctc cgaacagtac
     6301 gcccggttca gggagatcag ccaacatgag tagcacacgt cgagagttgc ccaatcggag
     6361 aaatcatcat acgcaaaagg tgcggattgc tgggcagcgt accgcctatg tcagcgtaca
     6421 tgaagaccag gcgccggcag agctcttcat acgtgtccgg ggcatcgatt gcacgtcgga
     6481 ggttgtgggc ctgtatgaca tcgtgggtcg gcttgcttcg ttggcgcttc agtacggcgc
     6541 tccgcttgag aaggtgggcg caatgctctt gggctcaaag ttcgagctag ccgggccggt
     6601 cacgttgcat ccgagaatca agaattgcac gtcgttgccg gatctcatcg gccgtcacct
     6661 gctggtcaag ttttgtaacc gcgcagacct tgcgcatgtg tctgaagaaa acaacgaagg
     6721 gaggaactaa ccatgccagc gatgatgatg gcgctcggtg gaggggcctt gagtgtcggg
     6781 ggtggaattg cgaaggcgtt tggcggtgac tcgcaagcga aggccgcacg taaagcggcc
     6841 cagatgtata tcgactacca ggaacgtgag cgcaaaaagt tcctggatgc caatagcccg
     6901 atccgcgaca agctcatgca gtactcgcaa ggcaatagcg ggtacgacgc cgatctggtg
     6961 cgcggcatga aggacgacgt gaacgaggac tacgggcagg gcctcgcgga tgtgtcgcgc
     7021 atctcggcga attcggcagt cggcaagaac tcaggcgggg gcaacgtcta cacgcctggc
     7081 cgctacgatc gggcgacgag acagctcgga cagaatctcg ccatgaacaa ggcgaagatg
     7141 atccgggacg tgaaccagaa gaatgcggac atggcgatca gcaatcagcg attcgccgtg
     7201 agtgcgcttc caacctactc accgggactc tcggccacgc cacagatcgg gtatgacgcc
     7261 ttccaagggt tgaatgcggt cgcgccggtt gggaactaca tcggcgacat gctgcaaagc
     7321 gtggggcagc cggccatgtc ggcgggaatg gatgggctcg tccgtggccc catcgccatg
     7381 aagctcgcac agatgcagcg gcaacaacag cagccgggac catgggaaaa cttttacacg
     7441 tcgccccatc tccagtactg agggattcat gaatcaagaa atcacacttc ctcaagaagt
     7501 gtctgacgag gtgttagtcg ggcgtcttcg tcagtgggca tcggagatga cgccgcaaga
     7561 ggctctcaat attttggcaa gcgggtccgc agaatcactg attgcctact gccgaagcca
     7621 gaaccaacca ttgcctaggg atccgcataa gctctggggc ctgttcgcag tcgaactcct
     7681 ggcacgtact gagagcatga atgcatcaca cggggccgcg ggaacggcct gaaacgttga
     7741 agcgcctgca tgagtaacct gcccttacag cgtgcggtgt ctccggggta cctcgatttg
     7801 agggctttgg cggtctatag ctcatcctct gtgcggtggc tccgggaccg catcaatgac
     7861 acgacagccc ccctgcccta tcatcgagtc ggcggcaaga tcctgattcg aaaagaggac
     7921 tttgacgact ggattagccg tttccggact attcaatccg caaccgaact agaaaccctt
     7981 gttgacggtg tgatggccga ccttctcacc aataaatctt cttgacgaac acattccata
     8041 catttatcct atacgctaaa tgcttctcag gacattacgc ctcaagcaag aattgactca
     8101 aaagcaactc gcagccaaag cgaagatgag ttacgccttt ctgtcgaaca tcgaaaccgg
     8161 taaagccgat ccatccctga gcacattgaa acgcttggcc gaggcgcttg gggtcagtgt
     8221 cagtgagttg gtgaaggaac ctcgctcaaa gaaatgaggc gcgtatggct gtaaaagtgc
     8281 gagaacgccc ggcgggtagc ggaaagtggt ggatctatac cgacttccgc gggaaacgga
     8341 cggcccgata tattccgcaa gggaagcgcg ccgctgaaaa ggttgcctca gagatcgctg
     8401 caaagctgga tctcattaag cagacggaac gccatgggct gaccgtttct ccgcgcgaag
     8461 tgatcacagg gcgtaaagat ctctcgcctg tgaagacagc tccggctgtc atcggccccc
     8521 tcttcagcga ttatgccgcc caatggctcg atgcgtgtga ggcccgcgga cttaaagcga
     8581 gtacctaccg atcctacaag acgattctga gcgtgcatct acttcctgcc tttggtcctc
     8641 tccatctgag tgaaatcgat cgacgagccg ttaagctgtt cgtacagacc aagctgactg
     8701 aggagctcgc agttggcagg aaagaagcac agaacaagaa accacgtgca cctcgcaccg
     8761 tcctacattt cttgcgagcc ctttccactc tatttaattc tgctatcgag gaccattatc
     8821 ctgttgtgaa tcctgccctc aatcctggca agctcttacg aacgaagcgc acgagcgagg
     8881 acatcaatcc attcgatcgc gacgaggaag ccacctttct tcaagcggtc caagaccgaa
     8941 caccacgcta ctatcccttt ttcctcacgc tgcttcgaac cggatgccga ctaggtgagg
     9001 cgattgccct ccaggcggga gacctcgatt ttcgaggcaa gtttatagaa attcgtcgaa
     9061 acttcaccaa tgggaatctc acgacgccaa agagtaataa accgcgccgg gtcgatatgt
     9121 cggacaagct catcgaggtg ttaagagacc atgttgtctc ccaggaattg gaagcaatgg
     9181 gtcggaatca accgaaacct gagtggctgt tcaccaatga gggtggccaa agactcgacc
     9241 ccgacaatgt gagaaagcgt gtgttttatc ggctgttgga aaaggcacag atccgtcgta
     9301 tccggatcca tgaccttcgt cacacctatg ccacccgtct ggtcagcaac aatgaaagtc
     9361 tcgcctatgt gcgggatcag atgggtcata gctcgataca ggtcacggtc gacctctatt
     9421 cgcactatgt gccaggcagt aaccggcaag cggtgaatcg gcttgatgag cgtgtggaga
     9481 agagagaacc ggagtccaaa tccgcaacca gccgcaacca agaatcggct gaggaatcgg
     9541 aatcccctaa gtcattgatt tctggagccg gcgagtggaa tcgaaccacc gacctgcggt
     9601 ttacgaatca ggccaggaca atttttgaaa cgctcggtgt atcaagtggt ttccccgttt
     9661 taactgcgaa tacaggcact tgcacgcatt ccactgattc gatggattcg agaggtataa
     9721 gtgcgttttg aaattgttta gcacaaattt agcacagcac aactcacgat gattcccagt
     9781 catactgcta tcgatttttc ctggcttgaa gggtattctt tgcgcaccta tgaactgggt
     9841 gccagtccag agaatgatta gctcatttgt tcctgtgagt gtgctcgagg ctaaacgact
     9901 gcaaaacgtt tttaatgagt ccagcctatt tttgcatcct ttcaaagatc cacttctgat
     9961 cgattttggc gcacaccgtt ggctttcgag cgctcgggag gaaagctatt ctgattggct
    10021 ggcgtggatt ctgactcaac ttcaaaggcc agatttggtg ttcggcgttc tattagaaaa
    10081 gtatgatgcc aaccttcagg agaaatgcaa acaaaggctg actgtgcatc gagaattagg
    10141 cctagttgat gaagatgggt ttcgaaagcg aaccgacctc gagttaatgt atggagctga
    10201 gaaagctgtc ctggtagagg tcaaaaaggg ggatgcgagt catattgacc ttgaacaact
    10261 aagagttcag cttcgccata aacccgggtt cggccattat gttttattgg cccgacatgg
    10321 cgagcaccgc caatatgctg acggatttca gttacgcaca tggaatgatg tatgtgtggc
    10381 tcttagacga atcgtggcga ttcctgagct gaaaggaacc gtcgaaggag ctatgatact
    10441 ggcatttgtt ggcgccgtag aacagaacct tcttggatac ccaggaaatt tctctgaacg
    10501 attcgcaaga ggcgagctaa ttagtgtttc gttaagacaa agaattgaag cccatctgaa
    10561 aagcacaata aatggttgat catctcgaga gagcagaatc gtactgccac ttcattaatg
    10621 aggggttacg ttcttacctc gatgctagaa ttgcgatagg agcgtttagt gatctgatat
    10681 tgaatgaatg cgcctcctcg atgcaaaaat tccttcctag tctcagcgag gtgttaggcg
    10741 ttaatttgca acaaaaagac attgggtata gtaccgaacc ttccggcgca aacaattccc
    10801 caagtgattg gaaccaaaac tttatctgtc tccaagcaaa aattgacctt agcaagaaaa
    10861 ggaatgattt gtaccagttg gcaactggtc tgtgttggga caagaatgac ggtccggtca
    10921 gtatttggcc gtatacatgt cttgtaaccc aaactttaga gaaggcgaat gcgctctaca
    10981 aaaccttggg caatgatgaa attgacaata ccgaaaacta ttgcctcacg atttacggcg
    11041 acagtccatc cctaaaagat ggttttcctt atgctgaatt agagtggacc ctatcggaat
    11101 ggatctcagt gctaaggaag acgaaagcat tattgaataa gacagcaaag gggtaatggg
    11161 aaaatccctg aagtacaaat cttattggct cgagcaatct caataaggtt cttccgctag
    11221 gttgatcctt gacacatgaa tctcgagtct gcgctctcct ctcgaaataa ttgcttataa
    11281 tgtcactcga cctccaacta ctatcttcga agctcactcg gtatagaaag caatttgact
    11341 cctctatcga cgaagtcgcg gctgcgacag gtatttccat agaagcattg tctgactatg
    11401 aacaaggacg ttcggagcct acaggggatg aaattctgat tctcgcggac tattacaagt
    11461 gcgactataa atttttcatt tccaatgaac agctggcagc ttttgagcaa acggagaccc
    11521 tattcagaag ctattcaaat gaactgtcga aagaggatag atgggcaatt caagaattcc
    11581 tatttttgtg tggttgcgaa gagttcctcc aaagagagtt ttctcacaag cgcacaccat
    11641 tcgtttttca gaaaactgga aaacactata aggcccatgc tgtaagtgct gctctcaatc
    11701 ttcgtcgata tctccaatac aaaaccaccg aagtaggccg ggatatttat aaggatttca
    11761 gatctatagg tttccatgta tttcgtcgtc atttgtcaaa ctctgcaata tctggtttgt
    11821 gtattcgcca tccatttgcc ggaaaatgca tattggtaaa cttcactcaa gacatctatc
    11881 gtcagcgttt tacagctgcc catgaagctg gtcatgcaat ccttgatgac gaccgagaca
    11941 ctgtcatttc attcacctgg gacaaaaaag atttatcaga aattcgagcc aatacgtttg
    12001 cgtctcgcta tcttatgccc cctgaatttc ttacattact tcctaagaat gcaaaatgga
    12061 atgacactgc ggtcgtcaag tgggcgacga atctgatggt tagtacagaa gccctcgcat
    12121 atgctcttaa agatgccggc attgtttccc aagaagaggc ggatgtaatt cgtgccgttc
    12181 ggattccgcg tcaggccaaa acggatccgg agttgcctga gtctcttcct cctcgcgcaa
    12241 gagaacgcaa agaagagttt atgcagcgag gactttcaga cttctatgtg aatttgtgtt
    12301 tcaaggccta tgatcaagat ctcatttctg ctggcaggct tggtgagatg ttattaatac
    12361 aagatcatga acttgccagc atcgctgact tgtatggtca acctttgaaa catggggatt
    12421 gatgtctcta ggttccatcc gcataacgtg accgacacgt gcgctgtctg gcatgtgttg
    12481 tcgtcgcaaa ttctgtacgc caccgctttg tcaatgggct gtcatttctg catcacgcag
    12541 ttcgttcttt acgagtgcct ttttaaacca agaacccatt cgctcatcgg cagagaactc
    12601 caatcgagat tacggcggga acagtccaag ggaatcttca aaagctaccc gttagctgtg
    12661 gaggatctcc aggatattgg cattctggag aaccgccaac gtcttagcaa aggagaactg
    12721 tcgtctatag cttttgccaa gaagaccagc caagcatttc ttacagacga tcagggagcg
    12781 agaaagctgg cacgcggtgt tgtgactcaa ggtgtccaga cgacacctca tcttttcggt
    12841 tggctcttct tttccggaca cttagcggat ggcgatagaa cggcgctcat tcgagaacat
    12901 gaagccatgg gagggcgact tagtcgctat tttgaagaga tgtacctgga aagcttacga
    12961 tgccgcctaa tggcatcaca ctcaaaaact gctgataaat agcgggatcg cttctggaga
    13021 gctcatttga agccctctga ttcgttatgt gggatatctt acagtgctca ggtgaggacc
    13081 tatataagcg atcgtaaggg ctcgtcgttc gacctgtggt tcgaagtata tcctccacgc
    13141 ctctggagtt atgcgggcgt gccagacaaa caatcgctgt cgtccgtctg ggcaaacaaa
    13201 agtccgctca gctccgtaca gttgcaaagt tggtccgctt tccttcgtaa ttttcccagg
    13261 aaatgatgaa ctaacaaaca tctcagccgt ccaattctcg caatactcct gaaaagcaag
    13321 aaggtgctta acgatctgaa taaaatgggg actactttgc gtgaggcctt ccaactgctc
    13381 caacgcagta gagcagaatt caagtgaggg gagcagttcc ccccgccgtc ttacgagatc
    13441 acgtccactc gagagcttcg aagtcgtata tcgttttatc catcccaagt tctcagttat
    13501 atgagagcgg tgagatgcgt gatgcacctt tccgttcgat atctctagat caccgtcagc
    13561 tgatagactc tgcactatta tttctagaat gctcttattc cagtcgggtt tcgactgtag
    13621 gcttatcgca actctattcc cttcaaaagc aaacccaagt cccttagccg cctgattatc
    13681 gaataggtat tcaggcggca aagagctttc gccctcacca tcttccaatt cgaccatgac
    13741 caatggagac ttagtaatca gcgacctaaa gtactgcctt tcttctcttg ggacgtcttc
    13801 gtcgtttctc cagcgggcca gtggataccc atctgcaatg ttgagagagt gtacgttggg
    13861 ctcgctgtgc aaaactctgg cgacgccgtg cgaagcggca aaggaagagg ttttgataag
    13921 ctctgacatc caagccctgg cctcaaaaag gttagccgct ttagccaacg acagctcatt
    13981 tagaaccatt tctaacgcca tatctaacta ctctagtagg ccttctaaac tcttatccca
    14041 ctcatcaaaa aatccgtctg gccaaggact gattcgacct tcactatcaa cacgtattgg
    14101 tgaaattcgc cttacgaact ctccatcaac ctctgcgcag gagaaaaaat acatcatcac
    14161 tttctcagga tcaaattgaa gccgcctaat agcgactctt attccgttca gtacatgatc
    14221 actatgtgtc tcaagaataa cttggacccc atctagagca gccctaacaa taagttcgcc
    14281 taatttaccc tgtcctctcg gatgaagatg agcctcagga ttttcgagaa gtaccagtcc
    14341 tccgggtcct gctgataaaa ggactgtcag caccggcaag gtataggtta gaccgaatcc
    14401 gacatttgta gaacgatacc gattacttaa ctgctttcca cttacaaaag aatattctaa
    14461 gttgacaagg tccatccctg gatgggtagt gaaatgggta cgaagccctg gactaaactc
    14521 ccccatccat gcctctattt gatccgttaa tttgaaagat tctgcgcgaa gatgccgcct
    14581 tgcttggcca gggatatcgg aacggccgaa cacggataaa aaatgtgaac aatactcacc
    14641 cttaacgcca atctgcctag tattcctaac aataaaatcc gacataggaa acgatgttct
    14701 aggaccaacc cgttcagcct gaagatagtg aaaattattt gaaaagagac cctgcgcgta
    14761 actttggagt gagacagagt cgggagttac cgatagaacg tcagcctccc tattatagtc
    14821 aaaccgccac cttgcttttg ctccatcgct aaagtgtaag tcataaccaa tgtactcttc
    14881 cttggcattc tcatacaggg catcacttgc gtttcctact gtcaccagat caccgttcaa
    14941 tgcgagtcct cgagtatcta gcagcccttg agagtgcgat tgtcttaaca gcagaagact
    15001 ctgtagtaag gacgatttgc ccatcccatt taaacctgcg aggagcgtca aagctccgaa
    15061 atcgaacctt tgtgattcga aacacttgaa attatgtatg tgcagtagtg agatcataag
    15121 agtgattgct ggattaactt ttgtatagaa ccaaagcgcg ttctgacccg agacggatca
    15181 ccagtgccct gagatataga ggcctcaaaa tctctctcca ataggagagc acagaacttc
    15241 tccagcacta tacccttccg agcaacaaga cgagcaactt catcttccga caatgctgcg
    15301 aggtttaccg accaggtctc gaacaatgcc ttgttgatta aggatctgcc agtccgacca
    15361 gcactcatct tccggaatgc ctgtctatca aaaatatggt gtgccgcttt cattgcattg
    15421 caaaactgac tggtcaaacg ttctatgcga tgacgaggag catgattgat ctcgatcatt
    15481 gctgcattca aaaaactgtc aaaatccgca gtcttataat cagttggggg cgtaagcgta
    15541 aaagcaataa atcgcaacac gcactcgcga tctaccattc gcttgctgtt tattcctcga
    15601 tcggtcgctg caaggaactc ttctgatgat gccagttcct ccagcaaggt ggttacctgc
    15661 ccctgattta gagcgtgcct aatttcttgc ggagaaagcg gcaggccccc agtattgatt
    15721 ctcttaaata tgttgaactt aacatcgggg ggagtgccct tttcaatcag gaatacagta
    15781 atctgcgttt catttattcg tcttttcaga aaccttggca gctcgtcgaa acgggctcca
    15841 ttcaactgtg ttaaaaattc gagtcctatg agcgctaact gcttgtcgat cacgaacttc
    15901 ttcagagcag tcagtcgctg caagccatcc acaactagcc agctattttc atctgttgcg
    15961 tccatataaa aagcaggcaa cggaattctt atcaataagg actccacaag tctgctctga
    16021 gctgtcgggc tccaaatatc agccattcgc tgaaaagatg gggagaggtt gatctccccc
    16081 tctttgatcc tcgttagtaa cagatcaacg ctcaaggttt ttgattcgac gcggatgaga
    16141 gtaggatcga aaggttttgt tataaagtcg tttacttcct ctaactctac tccgttctca
    16201 tcgctttcag atgacgtgaa tagttccatt ttgcgttcct tgggataccc tgcggatgtc
    16261 aattaatacc tcgcattttc ctcactgacg gaccagtcta atggaaatcc ttgggcttgt
    16321 taagaacaat tagcagcttc cttatcagtg gatagcggta acgtatccca gaattaccta
    16381 cccgaggatc aaaatctatg gagttatctt gactatttgt attatatttt gtattgcatt
    16441 gtttggaggt ccttaagatg aagtcagatg acgccaaaaa agaaactctt caatttaaag
    16501 tcacgggaca ggaacgcaag cttatcgaaa aatgcgctga agaagaagga accaccgtgt
    16561 ccaagtatgt tcgcggtgcg gtcttgatga gcctcgtgat ggacggaaag gccgaggcta
    16621 ttaagattgt ggcaagagag gtcggagaaa aggcctttgg cgtcgtccga cagaagctcg
    16681 tcagaccgac acaggaagga cgctgagaac agtgctatcc gtcacactat taagagacaa
    16741 agccggtccg cgccgcatgc cggtaggctg gggcgcggag cggaatggcc cccgtcttgg
    16801 taacacgggg gcgctaccca cagcgctcc
//
LOCUS       DAORQN010000023        15787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_246_length_15787_cov_19.687897, whole genome shotgun sequence.
ACCESSION   DAORQN010000023 DAORQN010000000
VERSION     DAORQN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_15787_cov_19.687897"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1224)
                     /locus_tag="PK614_02955"
     CDS             complement(472..1224)
                     /locus_tag="PK614_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81198.1"
                     /translation="MNRTVIALLTLVVLTTGACASKPPKKQFQRLAGEFVSPVHITKP
                     VDQQSAVLGITIKSPAWVPNTEDRLYLVKVENERDLFEGSKLIPTSILAPSPGVGGGG
                     TVYVQNVPPGRYAAVAFSTSEKGFGKVREVTLFLLPKTVVKQSDTTVVPGSISFMGEY
                     EVGTSTMVLQEKAADPAQEHYFEAFWGKPLAQVIADLQMIGTPAYFAVHTVSVKQGSR
                     DAAAEERFLAAAKRDLEPAWAPVIERRRTAVK"
     gene            complement(1443..2705)
                     /locus_tag="PK614_02960"
     CDS             complement(1443..2705)
                     /locus_tag="PK614_02960"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81199.1"
                     /translation="MNIGLNARALTIGLAFLGVVSGCAVDQRWRSAMQEGNHALRSGD
                     YTHAEESFVAARKEAEVLDPQGKRLAETLSQLGEVNRELGRFPRAEALFQEALAIRER
                     VYGPAHAETAASLTDLGELYRLQGLYTQSEALHQRAREIREQVFGADHSKTAESLNNI
                     AVVYQDQRRFADAEPVLQRALGILEKQLGPEHSTTAITRDNLAKMYQAQGQHARAMPL
                     YQRALTIHEKAFGPNHPIVARNLENLADTYRAQNQYPQAEVLYQRAVSIFKKSLGNDH
                     VDTAEAMSRLGQLYELQGLYSQAEPLFQQAIAIREKQQGAESPHVAGELKNLAGLYQS
                     QNKLEQSEDLYKQSLVIYEQSVGYDREAYVKALKSYASLLRRRQRSGEAERLEERAKA
                     VLKRLSLESQSQGKTAADVPLAPVSPLP"
     gene            complement(2946..4961)
                     /locus_tag="PK614_02965"
     CDS             complement(2946..4961)
                     /locus_tag="PK614_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB81200.1"
                     /translation="MANSTLTTPDSRPLRIALIGAGRHAQHHARAILRCPGVQLVAVA
                     DPSDAAQAAMRDIVPGIGCFKTPEELFASERLNVVHIITPPASHAPLARMAIKAGCHI
                     YVEKPFTESVEDAQQILDEASAKGLRVCAGHQLLYEPPTRVLTQYLPSIGRVVHVESY
                     FSFRTVRHAPGGRKVLRADHQLLDILPHPVYLLLQVLEQAGEGRTELLSLEVSQAGTV
                     HALVRRGGVTGTLIVTLEGRPVESYLRVVGKNGSLFADYVRSTTQRAIGPGSSGIDKL
                     FAPYRQAWQLLIGTTSAMANRFLKSQRSYPGLAELFTAFYESARTGGPSPLSPESLLE
                     TVRICERVAKALKVGEAKALAAAAPKPVESRGVLVTGGTGFLGKEIVRTLLSRGRPVR
                     VVARREPSPWERIAGAEYVVADVATGAAAQLFKGVDTVIHAAAETAGGWPEHQRNSLD
                     ATEQMVRGADAAGITHFVHVSSLAVLAQGNGRPIGDDHPLEPDSKGSGPYVWGKLESE
                     RLAVQLGKELGLSVKVIRPGALVDYRDFDPPGRLGKRLGNIFVAVGSPGDRLGVVDVG
                     FAGRFLGWMTDAWDNVSSPLNLLDPVSPTKQELLDRLRQANPDLTVLWLPRFVLVPLS
                     WLATLAQKILRPGKPAIDVAKVFSVLPYDTSGIAKLAPQVDHASEKR"
     gene            complement(5108..6103)
                     /locus_tag="PK614_02970"
     CDS             complement(5108..6103)
                     /locus_tag="PK614_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB81201.1"
                     /translation="MEPASILIVEDEPVVAKDIQLSLQRLGYRVPATATSGEEAIRKA
                     VDMHPDLILMDIVLKGKMDGVETALQIQRKQDVPVIYLTAYADNHTLERAKVTSPAGY
                     MLKPYQAHELRPTIELALHRAQHERHMRERLRWIATTMRCIGDGIVTTDRGGRVAYMN
                     PAAESLTGWSQDDAAGMGVTALLGFHEAGPGYESESPVQQAMTQVRIVAIEEALLISK
                     QGGRRTIRGSVAPVADDGGNVLGAVLVFHETASGERGLQASDRQGDTLWKMDAQLGRP
                     QGIINLCSWCKRVPDESGEWYDLATFIAERSAIQFNGGLCPECMDQCFPCDGRHK"
     gene            complement(6109..7941)
                     /locus_tag="PK614_02975"
     CDS             complement(6109..7941)
                     /locus_tag="PK614_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB81202.1"
                     /translation="MTIPASFFLGMSRSSYSRLGLALALIAVIGAIDWWLPLGLTITT
                     LYVVPVLIASRIPHPRLTFWVAALASLVTILDMFDRPLFALTWVSAMNRAFALMVIWV
                     TALLCLRRQRDEAELLRINEDIEQQVQERTADLAAANQELEVLRAEAVSELVAIVKSS
                     DDAIVGMTLNGMIQSWNRGAERVYGYRAEEVLGRPISVLCPPNRLDEVPTMLDRIARG
                     EHVRNVEMVQRRKQGERIDVSLTISPVKDADGCVMGASAIARDVTVKRRIEAALRESE
                     ARFRMMADTAPVMVWMAGPDTHITFINKRWLEFTGRTVQEEIGDNWFTGIHADDLDRC
                     RKSYLQAFKSEQPFFLEYRLRRHDGEYRWIMDTGVPLFDEEGQFGGYIGTCMDLTERK
                     DMEDQLRRMLKEKESLLREVHHRVKNNLQVISSLLNLQSASIKDPVVNQLFRECQVRI
                     TSIALLHETLHRSHDLSRIKMGDYIRTLTGHLFRSYGVDPNLISLELNVDDAEFDIDT
                     GLTCGLIIDELVSNCLKHAFIDDSGGTVHIDLLDHVDGTFTLCVSDNGIGIPKDGVLN
                     NPDSLGLELVTLLAEKLDGSTELRSGAGTEWQIRFQQLQYSERV"
     gene            complement(8476..9594)
                     /locus_tag="PK614_02980"
     CDS             complement(8476..9594)
                     /locus_tag="PK614_02980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81203.1"
                     /translation="MNIPLLDLKAQYQSMRSEILAAIEATCDEQGFILGPRVVALEQA
                     VATYTGSAHGVGVASGSDALLLALMALGVKAGDEVVTVPFTFFATAGAISRLGAKPVF
                     IDIRPDDFNMDPALLERAITKRTKAIIPVHLFGQCAEMDAINAIARRHKIGVIEDACQ
                     AIGARQNGRHAGVLGDVACFSFFPSKNLGGFGDAGMVTTNDKALAESIAMLRVHGSRV
                     RYVHEAIGINSRLDALQAAVLLVKLKRLDEWAEGRRRNAARYVQLFTEAKLTDRIALP
                     VVGANNFHVFNQFTIRVHKRDELRSYLKDQGVGSEVYYPLPLHLQNCYRDLGYQKGAF
                     PQSERAAEEVLSLPIYAELSDEQLQYVVQAIASFYRKR"
     gene            complement(9616..10806)
                     /locus_tag="PK614_02985"
     CDS             complement(9616..10806)
                     /locus_tag="PK614_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81204.1"
                     /translation="MHVVCHVITKLELGGAQEVAMQVVSGLDRRRFRPVLLAGPGGLL
                     TDEAHALKGVEVRTIPSLVREIRPLQDLCALWELVGAFRRLRPKIVHTHSSKAGILGR
                     VAAWLAGVPCILHTIHGYGVTPAQPFWQQKALIALERMVGRITTHWVAVSQTDRRQGI
                     EWGLFTASKVSVVRPGIDPTAFAARIESPERDRLRAMLGVAPDQLLVGTVSCLKPQKS
                     PEDFVRVAALVCQRVPAARCVLVGDGALRPKVEALLLAQGLQDRVTLLGWRRDVAALL
                     KTFDVFVLTSQWEGLPCAILEARASRIPIVATRVGGAAEAIVEGIQGTLCPAGDVRAM
                     AERVCQILEDERFRVDLRTGTQELPEEFTIQETLKQYQSLYAYLLHATRPSEDVMKLQ
                     PNRL"
     gene            complement(10820..12130)
                     /locus_tag="PK614_02990"
     CDS             complement(10820..12130)
                     /locus_tag="PK614_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB81205.1"
                     /translation="MILIVGAGLAGLSAAYHLRGMPYKILEREREVGGLCRSYVKDGF
                     TFDYTGHLLHFRQTAIKALVESLLPDQLQRHARKSYVYSHDTYTEYPFQVNTHGLPPE
                     VVRECLLGFIATLSNPSSKSPAESRSFKQWIVESLGEGIAKHFMVPFNEKLWQVPLDE
                     LTSDWVSWLVPKPDVKDVVNGALGIKDKAFGYNPSFQYPATGGIKVLPEAFLPSVENL
                     SYDSELVEIETGRRRAVFRSAQGEWTEAYDRIVSTVPLPDLVRRCVDLPASLRELAAS
                     LRWVSVYNVNLAVARERISDKHWIYFPEHRYSFYRAGFPMNFSQSMGQPGCSSLYVEI
                     SHQPTERESDAALIERVRRGLEQAGVLQRSDEVVMSDVKDLYYAYVLFDRYRGRAVQE
                     LLAELERRGISSIGRYGLWEHTSMEDAIAQGQQAAARLRMKAAA"
     gene            complement(12127..13185)
                     /locus_tag="PK614_02995"
     CDS             complement(12127..13185)
                     /locus_tag="PK614_02995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRB81206.1"
                     /translation="MILLTLTFVVAMLLSIYGVPIARRAALKFGIVDNPDGRLKHQRE
                     PVPYLGGLAIYLAFLVSLAFTFEFRQDVLGIVLSGTLIVMLGLIDDFGVLSPGTKLAG
                     QFLAVFVLIKSGIRIEIASLPDWVDIVLTVFWMIGIINAFNLLDIMDGLSAGVGVISA
                     AFLCVVAILNGDQAIAFMLAALMGSLLGFLRYNWRPASIYMGDSGAMFIGLMLGALSM
                     IGKYTTGHSVSLLTPVLILGMPIFDTLFVMYIRFLRGLPVFLGSPDHLAIRLRHWGLS
                     VTQVVLLSYLGAALLGGIGLLVMAVSQDLAVVLSGVTVLCLAVAAVALTRVNVSTGAV
                     AVSTEAIPARSTERTGVV"
     gene            complement(13182..14318)
                     /locus_tag="PK614_03000"
     CDS             complement(13182..14318)
                     /locus_tag="PK614_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81207.1"
                     /translation="MTVTYFSKSSSIGPSSRYRVFQFLPHFQAAGIGCRVEALFGETY
                     FSILKVHPRALRTLLKIPYVLICFLRRLWTLLTLGKRDLIVIEGQLFPYAPPLAERLL
                     RWCRYRVAIEMDDAIYLTPGHEKKIPALLSMATGAIVGNDRLAAYAKQFSPRVCVVPT
                     VVDTERFKPDSTRSTGSSAQNSDAITIVWIGLAYNLKYLDVLAPALRALQSRFRLTLR
                     VVCSRPPHMPGVEIEFRPWDFQREVADLQDATIGVMPLEDTEWARGKCGLKLLQYLAV
                     GVPSVASPVGVNRDIIANGENGFLAATEQEWYERLETLCRQPQLRARMGQAGRRTVEE
                     QYSLAVWGPRLGDVYRTLAGNEHVNEIHRAVHSPVSGHRTTGTR"
     gene            complement(14503..>15787)
                     /locus_tag="PK614_03005"
     CDS             complement(14503..>15787)
                     /locus_tag="PK614_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81208.1"
                     /translation="CYLAPEAMSSVITRFQECPDLDFLYADEDMVRENGEFLDPFFKP
                     CWSPDLLIGMNYVGRFNVMRKQLLLDIGADWGDIANDGFYHLVLRVAEETNKIGRVPQ
                     VLSHCHITDESSDITAGQSELDVTAQMKSLQDALNRRGESADVVRDGAGKIKVHYRVS
                     RNPLVSIIIPTKDRWDMLRQCIKSVESVTRYPSYEIVVVDNGSRTAKAKEYLSTLSKK
                     WIIHRFPGPFNFSEINNYGASKANGEFLLFLNDDTQVIAPEWMSAMVAQAQRTGVGAV
                     GAKLLYPSGMIQHAGVVLGIRGVAGHAFRHLRGDTQHYRGLPHMLRNCSAVTAACMLV
                     SRDVFRQVNGFDERLAVEFNDVDLCLRIQQQGYRIVYTPEAELYHHENATRRGRRAPD
                     DEARFRLKWGEFIMKGDPYYNPNLTQLREDWSLKL"
BASE COUNT         3429 a         4982 c         4313 g         3063 t
ORIGIN      
        1 gtattcatga atagtccggg ctagagcgca tccgcagaca aacagtcgag acgttcttga
       61 acgcagcttg cgcacagagt tgcgtgcggc cggtgtctca gttgtatgaa ggggatctcc
      121 ttccgacact ccaggcacag accagtaatc ctcggccttg cctgacggag agacaaaata
      181 tcctggcggc gcgggcgaga cagatacatc tgaatacctt gctttacgag tcgcacgcta
      241 cacctcctgt gctgaaagca acctggtcac catcagccta cggatcaaag gtcacaggca
      301 tgtccggaca aggtaaattt ttcgttaaca ggtgagggtc gctggagcaa agagttgtat
      361 tccgctggaa ggcgcccctg cgttctgtgc gcaggggctt cgattcagac catcacacga
      421 tcggggcaca caacggagcg gcgctgcatc ggatgcatac ctaaccccgc cctacttcac
      481 cgcagttcga cgacgttcga tcaccggcgc ccaagccggt tcaaggtcgc gcttggcggc
      541 tgccaggaac cgctcttcgg cagcggcgtc ccgactcccc tgtttgacgc tgaccgtatg
      601 tacggcgaaa taggccggcg tgccgatcat ctggagatcg gctatgacct gggccagcgg
      661 cttgccccaa aatgcttcga aataatgctc ctgcgccgga tcggccgcct tctcctgcag
      721 gaccatcgtg gacgtgccga cttcgtactc ccccatgaaa ctgatgctgc caggaactac
      781 ggtcgtatcg ctttgcttca cgaccgtctt gggtaatagg aatagagtga cttcgcgtac
      841 cttgccgaat cccttctccg aggtgctgaa tgcgacggcc gcataccgcc cgggcgggac
      901 attttgcaca tagaccgtcc cgcctccccc aaccccggga gacggagcca gaatgctggt
      961 tggaatcagc ttggaacctt cgaagagatc acgctcgttc tcgaccttca ccaaatacaa
     1021 ccgatcttcg gtgttaggta cccaggccgg actcttgatc gtgatcccga ggaccgcact
     1081 ctgctgatcg accggcttcg tgatgtgaac cggcgagacg aattcacccg ccagtcgctg
     1141 aaactgtttc ttcggaggct tactggcaca ggccccggtg gtcagaacga ccagggtcag
     1201 cagcgcaatc accgttcgat tcatggaggt ccctctgggt ggattaactg agcagttcgc
     1261 ggccacagtg taggggatta ggcgtactgc ctcaagcgat acttccggcg ggtatctgcc
     1321 ttgaactgac tccatgcgag agctgggcga tagatcgagt acagggcgta cggtcagggg
     1381 gtaataacgg agcagaaatt tacagcaatg ggaaggtagg aatgggcagc acaatggcta
     1441 ccttatggca acggcgaaac cggagcaagt ggaacatccg cagcagtttt cccttggctc
     1501 tgactctcca gtgagaggcg cttcaagacc gccttggctc gctcctccag gcgctcggct
     1561 tcgccggacc gctgccgacg cctcaacagg gacgcataac tcttcaaggc cttcacgtag
     1621 gcctcgcgat catacccgac ggactgctcg tagattacca gcgactgttt atagagatcc
     1681 tcggactgtt ccaacttatt ctgcgattga tagagtcccg ccagattctt gagttcaccc
     1741 gcaacgtgag ggctctctgc gccctgctgc ttttcccgga ttgcaatcgc ctgctgaaag
     1801 agcggctccg cctgagagta gagtccctgc aactcataga gttgtcccaa gcgactcatc
     1861 gcctcggcgg tatcgacatg gtcgttgccg agagactttt taaaaatact gactgcgcgt
     1921 tgatacagca cttcggcttg agggtattga ttttgggccc ggtacgtatc cgcaagattc
     1981 tcaagattcc tggccacgat cggatggttg ggaccgaagg ccttttcatg aatcgtcaag
     2041 gcgcgctgat acaacggcat ggcgcgagca tgctgcccct gcgcctgata catcttcgcc
     2101 aggttgtcgc gcgtgatggc cgtcgtgctg tgttccggcc cgagttgttt ttcaagaatc
     2161 ccgagtgcgc gctgcaagac gggctcggcg tcggcgaagc gtcgttgatc ctgatagacc
     2221 acggcaatat tgttcaaact ctcggctgtc ttgctatggt ccgcaccgaa cacttgctcg
     2281 cggatttccc gtgcccgttg atgaagcgct tcggactgcg tgtagagtcc ctgcaggcgg
     2341 tagagttcgc ccaagtcggt caggctggca gccgtctcgg catgggccgg cccatacacg
     2401 cgctccctga tcgccagggc ttcctgaaag agtgcttcgg cgcgtggaaa ccgccccaac
     2461 tcacgattca cttcgcccag ctgactcaac gtctcggcca aacgtttccc ttgtggatcg
     2521 agcacctctg cttccttgcg agcagccaca aaggattcct ccgcatgcgt gtagtcgccg
     2581 gaacgcaggg cgtgattgcc ttcctgcatg gctgaacgcc agcgctggtc taccgcgcat
     2641 cctgagacga cgcccagaaa ggccagaccg atggtaagcg cacgcgcatt caatccaatg
     2701 ttcatgatga tcaactcttc ggtggtgaga cggttgccgt ttggggactg gccttgctcg
     2761 tagaatactc ctaagactta ccccctctga cgcctcaaat caaccccacg agggcaactt
     2821 tttcgacccc tccggcatcg ctatattttt caaccacttc ccctaatgca cacatttcga
     2881 ccacagagga tgaaacggcc ccatgccgat gattaataat gaaacggacc atgcggcaga
     2941 gacggttatc gtttctcgga ggcgtgatcg acctgagggg caagcttcgc aatcccagag
     3001 gtatcgtagg gaaggacgct gaacaccttg gcgacatcga tagccggctt cccgggacgc
     3061 aggattttct gcgcgagcgt cgccagccag gagaggggaa ctaacacaaa cctcgggagc
     3121 cagagcaccg tgagatcggg gttggcttgc cggagacgat ccaacagttc ctgcttggtc
     3181 ggggaaacag gatcgaggag gttcaagggg ctcgacacgt tgtcccacgc gtccgtcatc
     3241 cagccaagga agcggccggc aaatccgaca tctaccaccc ccagacgatc gcccggcgaa
     3301 ccgacagcca cgaagatatt ccccaaccgc ttaccaaggc ggccaggcgg gtcgaaatcg
     3361 cgatagtcga ccaatgcccc gggacgaatc actttcacgg atagaccgag ttccttgccg
     3421 agttggaccg ccaaccgttc ggactccaac tttccccaga catagggacc ggaccccttg
     3481 ctgtccggct ccaacggatg gtcgtcgccg atgggccggc cgttgccctg cgccaatacg
     3541 gcaagactgc tgacatggac gaaatgcgta atccccgccg catcggcccc gcgaaccatc
     3601 tgctcggtgg catccagcga gttacgctga tgctccggcc agccaccggc ggtttctgcc
     3661 gcagcatgaa tgacggtatc cacccccttg aagagctgtg cggcggctcc cgtggccaca
     3721 tcggccacca catattcggc gccggcaatc cgctcccagg gggacggctc acgtcgcgcc
     3781 acgacacgaa cagggcggcc tcgcgacagc agcgttcgca cgatttcttt tcccaaaaaa
     3841 ccggttccac cggtgaccag cacgccccgg ctctcgaccg gcttcggcgc ggcggcagcc
     3901 agagccttgg cctcgccgac cttcagcgcc ttcgccaccc gctcacagat acggaccgtc
     3961 tcgagtaaac tctccggcga caagggcgac ggcccgcccg tccgcgcgga ctcatagaaa
     4021 gcggtaaaca gttcggccag cccgggataa ctccgctgac tcttgagaaa tcggttcgcc
     4081 atcgcggagg tcgtgccgat cagcaattgc caggcttgcc ggtagggcgc aaataattta
     4141 tcgatgccgg acgacccggg accgatggcg cgctgagtgg tactgcgaac atagtcggcg
     4201 aacaacgacc cgttcttccc gacgacccgt agataactct ccaccgggcg cccttcgagt
     4261 gtcacgatca acgtgccggt cacgccgccg cgtcgcacca acgcatgcac cgtcccggcc
     4321 tgactcactt ccaaggacaa cagctcggta cggccctccc cggcttgctc caacacctgg
     4381 agcaacagat agaccggatg cggcagaata tcgagcaact ggtggtcggc gcgtagcacc
     4441 ttgcggccac ccggcgcatg acgcacggtc cggaaggaaa agtagctctc gacgtgcacg
     4501 acgcgcccaa ttgacggcaa gtactgggtt aatacccggg tgggcggttc atacaacagc
     4561 tgatggccgg cacagacacg aagacctttt gcgctcgcct catccaggat ctgctgggcg
     4621 tcctccaccg actcggtgaa gggcttctct acatagatgt ggcatccggc cttgatcgcc
     4681 atcctggcca acggagcatg ggaggccggc ggagtaatga tgtgcaccac attgagacgt
     4741 tcggaggcaa acagctcctc cggcgtcttg aaacaaccga tgcccggaac aatatcgcgc
     4801 atcgccgctt gagccgcatc ggacgggtca gccaccgcca ccagttgcac gccggggcag
     4861 cgcaaaatag cgcgggcatg gtgctgggca tggcgacctg caccgatgag cgcgatccgt
     4921 aacggacgcg agtctggagt tgtcagcgta gagttggcca caagtcctgt gtgagtgtgc
     4981 agcgctacta tagtctattc tcacgcattc ttgaagcctt ctcgaaaatt agcgcaggga
     5041 ggaagactgc ggcggtgggc gcaacgtgcg agggacctcg tgtgaacagg tccacacaca
     5101 tctcgaacta cttatgcctt ccgtcgcagg gaaaacattg atccatacat tccgggcaga
     5161 gcccgccgtt gaactgtatc gccgatcgct cggcgataaa cgtggccagg tcataccatt
     5221 cgccggactc gtccggcaca cgcttgcacc aggaacacaa attgataatt ccctgcggac
     5281 gtccgagctg cgcatccatc ttccagagcg tgtccccctg cctatcagag gcctggaggc
     5341 cccgttcacc ggatgcggtc tcgtgaaaca ccagcacggc gccgagcacg ttccctccat
     5401 cgtcggcgac cggcgcgacg ctgccgcgaa tggttcgacg tccaccctgc ttggagatca
     5461 gcagggcttc ctcgatcgcc acaatgcgca cttgcgtcat cgcttgctgt accggactct
     5521 ccgattcata tcccggcccg gcttcgtgaa aacccaggag cgccgtcacg cccatgcctg
     5581 ccgcatcgtc ctgactccac cccgtcaggc tctcggcggc gggattcata taggccactc
     5641 gcccgccgcg atcggtcgtc acgatgccgt cgccgatgca acgcatggtc gtggcaatcc
     5701 accgcaagcg ctcgcgcatg tgccgctcat gctgggcacg atgcagcgcc agttcaatcg
     5761 tgggcctcag ttcgtgtgcc tgatacggct tcaacatgta gccggccgga gacgtcactt
     5821 tggcgcgctc cagcgtatgg ttgtcggcat aggccgtgag gtagatgacc ggcacatcct
     5881 gttttcgctg aatctggagg gcggtttcca ctccgtccat tttccccttc aacacaatat
     5941 ccatcaaaat caggtcagga tgcatgtcga ccgccttgcg gatcgcctct tcgccggagg
     6001 tcgccgtagc cggcacgcga taacctaagc gttgcaggct cagctgaatg tccttcgcca
     6061 caacgggctc atcttccacg atcaagatgc tggctggttc catggcgttc acaccctttc
     6121 tgaatattgc agttgctgaa agcgaatctg ccattctgtg ccggcgccgc tgcggagttc
     6181 cgtgctgccg tccagcttct ccgccagcag cgtcacaagt tccagcccca gtgaatcggg
     6241 attgttcagc acgccgtcct tgggaatccc gatgccgttg tcgctcacgc acagggtaaa
     6301 cgtgccgtcg acatgatcga gcagatcgat gtgcaccgtt ccgccgctgt cgtcgataaa
     6361 cgcgtgtttc aaacaattgg agaccagctc atcgatgatc aacccgcagg tcaaccccgt
     6421 gtcgatatcg aactcggcat cgtctacgtt cagttccagc gaaatgaggt tcggatcgac
     6481 cccgtatgaa cgaaacagat ggccggtcag ggtgcggata tagtcgccca ttttgattcg
     6541 cgaaagatcg tgcgaccgat gcaacgtttc gtgaagcagc gcgatcgaag tgatccgcac
     6601 ctgacattct cggaacagct ggttgacgac cgggtccttg atggaggccg actgcaaatt
     6661 caacaggctg gaaatgacct gcaggttgtt cttgacccga tggtgcactt cccgcagcag
     6721 gctttctttt tccttcaaca tccggcgtag ctggtcttcc atgtccttgc gctcggtcag
     6781 gtccatgcag gtaccgatgt acccgccgaa ctgtccctcc tcgtcgaaca agggtactcc
     6841 cgtatccatg atccaccggt actcgccgtc gtgacgccgg agccggtatt ccagaaagaa
     6901 cggctgctcc gacttgaacg cctgcagata cgacttccgg caacgatcca ggtcgtcggc
     6961 gtgaatgcct gtaaaccagt tgtcgccgat ctcttcctgc acggtgcgcc cggtaaattc
     7021 cagccagcgt ttattgatga aggtgatgtg cgtgtccggt ccggccatcc acaccatcac
     7081 cggcgccgtg tccgccatca tacgaaaccg ggcctcgctt tcccgcaaag ccgcttcgat
     7141 acgccgcttc accgtgacat cgcgcgcgat ggccgacgcg cccatgacac agccgtccgc
     7201 atctttcacc ggcgagatcg tcaacgacac atcgatgcgc tctccctgct ttcgccgctg
     7261 caccatctcc acattgcgca cgtgctctcc ccgggcgatc cggtcgagca tcgtcggcac
     7321 ttcatccaat cgattcgggg gacacagcac agagatgggc ctgccgagca cctcctcggc
     7381 gcgatacccg tagacccgtt ccgccccccg attccaactt tgaatcatgc cgttcaacgt
     7441 catgcccaca atggcatcgt ccgatgattt gacgatcgcg accagttcag acaccgcttc
     7501 cgctcgcagc acttcaagtt cctgattggc ggcagccaga tccgccgtgc gctcctgcac
     7561 ctgttgctcg atatcttcat tgatacgcaa caattcggcc tcgtcgcgct gccgacgcaa
     7621 acacaacagc gcggtcaccc aaatcaccat gagcgcaaag gcccgattca tggcgctgac
     7681 ccaggtcaat gcaaagagcg gccgatcgaa catatcgaga atcgtcacca gagacgcgag
     7741 ggcggccacc cagaacgtga gacgggggtg aggtatccgg gaggcgatga gaaccggtac
     7801 tacgtagagc gtggtgatgg tcaatcccag gggcaaccac cagtcaatcg cgccgatcac
     7861 cgcaatcaac gccagggcca atcccaacct ggagtagctg ctccgcgaca tgccgagaaa
     7921 gaatgatgca ggtattgtca tacgtacaac gacgcggcgt ctccctcatc atccatatga
     7981 gcaatcccta tgcctccggc gccacatcca cccgccaatc gcacggggaa acagacctca
     8041 cttggcaacc aagatctggt gataggcggc caagccttga ccggtgccgc ttgccaggat
     8101 tgtccaggct gcgaccggtt ctcatattga gcccaacgtc ggcgtcgtag gaattccatt
     8161 cagcgcaagc ccatgcccca cagccggccc tggtgtacgt caccgcctca tggttgttca
     8221 aacttgaacg ccaggctacg ggagcgcttg tcgtcgattc gatgaccgga agggaggcac
     8281 acatgcggtc agtcaagccg gaggaggcgc cgtcggcggc ggcgtcacaa cgtgcaacag
     8341 gcaacgacag tcacagggcg acacgccagg aggcactgct tctccggcgg aggactgtga
     8401 aactgtcggc tatcaggatg tgaaaaagaa ccgtccggcc cggaaggaaa ccggcgccgg
     8461 acggcaacgc gaatgttagc gtttgcggta gaacgaggcg atcgcctgca cgacgtattg
     8521 aagctgttcg tcggaaagtt cagcgtagat gggaagcgag agcacttctt cggccgcacg
     8581 ttccgattgc gggaacgcgc ccttctgata ccccagatca cgataacaat tttgcaaatg
     8641 caggggcagg ggataataga cctctgaacc cacgccctgg tctttcagat aactccgcaa
     8701 ctcgtcacgc ttgtgaactc gaatcgtgaa ttgattaaag acgtgaaagt tgttggcccc
     8761 cacgacgggc aaggcgatcc ggtctgtcag tttcgcctcc gtgaacaact gcacatagcg
     8821 cgcagcattc cggcgacggc cttccgccca ctcatccaga cgtttcagct tcaccaacaa
     8881 gaccgccgct tgcaacgcat ccaatcggct gttgatgccg atcgcttcgt gcacatagcg
     8941 cacccggctc ccatgaaccc gcaacatcgc gatggactcc gccagcgcct tgtcgttggt
     9001 ggtcaccatg ccggcgtccc cgaaccctcc gaggttcttt gacggaaaaa aactgaagca
     9061 ggccacatcg cccaacacac cggcatgccg tccattctga cgggcaccga tggcctggca
     9121 ggcatcctcg atcacgccga tcttgtgtcg tcgggcgatc gcattgatcg catccatctc
     9181 ggcgcattga ccgaagaggt gcacgggaat gatcgccttg gtccgtttcg taatggcccg
     9241 ctccaacagc gccggatcca tattgaaatc atcagggcga atatcgatga acaccggctt
     9301 ggcccccagc ctggaaatcg cccccgccgt cgcaaagaag gtaaagggca ccgtgaccac
     9361 ttcatcgccg gctttcaccc ccaaggccat cagcgccagc aacagcgcat cgctgccgga
     9421 ggcgactccg acgccatgag cactgccggt atacgtcgca accgcctgtt ccaatgccac
     9481 cacccgcggg ccgaggataa agccctgctc atcgcaggtg gcctcaatcg cagccaggat
     9541 ttcactgcgc atcgattgat attgggcttt gagatcgagc agcggaatgt tcatcgtatc
     9601 ccccggttcc cccttctaca gtcgattggg ctgcaacttc atgacatctt cactcggacg
     9661 cgtggcatgc aggagatagg catagagcga ctggtactgt ttgagcgtct cttgaatcgt
     9721 aaactcctcc ggaagttcct gcgtacccgt ccggaggtcg acgcgaaagc gctcgtcttc
     9781 caaaatctga cagacccgtt cagccatagc ccgcacgtcg ccggccgggc agagcgtgcc
     9841 ttgaatgccc tccacgatgg cctcggcagc gccgcctact cgcgtggcca caatcggaat
     9901 ccggctggct ctggcctcca ggatggcaca gggcaaccct tcccattggg acgtcaggac
     9961 gaacacatcg aacgttttca gcaacgcagc cacatcgcgg cgccagccaa gcagggtcac
    10021 acgatcctga agcccctgtg ccagcaacaa cgcttcgact ttcggccgca aggctccgtc
    10081 gccgacgagc acacaacgag ctgcgggaac acgctgacac accagcgcag caacgcggac
    10141 aaagtcctcg ggagattttt gcggtttcaa gcacgagacc gttcccacca gtagctgatc
    10201 aggggccact cccagcatgg cccgaagacg gtcacgttcc ggcgattcaa tacgagccgc
    10261 aaaggctgtc ggatcgattc ccggcctcac caccgagacc ttggacgcgg taaagagccc
    10321 ccactcgatg ccttgccggc ggtcggtctg cgacaccgca acccaatggg tggtaatccg
    10381 accgaccatc cgttccagcg caatcaatgc tttttgttgc cagaacggct gcgcgggcgt
    10441 cacgccgtat ccgtgtatcg tgtgcaggat gcaggggaca ccggcaagcc aggcggccac
    10501 ccgccctagg ataccagctt ttgaactgtg ggtgtgcaca atctttggcc ggagtcgtcg
    10561 aaacgcgccg accagttccc acaatgcgca cagatcttgg agggggcgga tctcacgaac
    10621 gagcgacggg atcgtgcgaa cctcgacacc tttcagcgca tgcgcctcgt cggtcagcag
    10681 cccacccggc cctgcaagaa gcaccggccg aaatcgtctc cggtccaggc ccgacacgac
    10741 ctgcatcgcc acttcctgcg cgccgcccaa ctccagtttc gtaatcacgt gacagactac
    10801 atgcatgcca ttcacccgct caggccgcgg ctttcatccg caatcgcgcc gccgcctgtt
    10861 gcccctgggc aatcgcgtcc tccatcgacg tatgttccca caatccgtac cgcccgatcg
    10921 acgagatccc gcgtcgctcc aactcggcca gcagctcttg caccgcacgg ccccgatacc
    10981 ggtcgaacaa gacatacgcg taatagagat ccttcacatc cgacatcacc acctcgtcgc
    11041 tccgctggag cacccccgcc tgctccaacc cccgccggac tcgctcgatg agcgcggcgt
    11101 cggattcccg ctcggtcggc tggtgcgaga tttccacgta gagggaactg cacccgggtt
    11161 gccccatgga ctgagaaaaa ttcatcggaa acccggcgcg atagaacgaa tagcggtgct
    11221 caggaaaata gatccaatgc ttatcggaaa tgcgctcacg ggcgacggcc agattcacgt
    11281 tatacaccga gacccatcgc agcgacgcag ccaattcccg cagcgatgcc gggagatcca
    11341 cacaacgccg caccagatca ggcaggggga ccgtcgacac gatccggtca taggcttccg
    11401 tccattcgcc ctgtgcgctg cgaaataccg ctcgccgcct gcccgtctcg atttctacca
    11461 attccgaatc gtacgataag ttctcgaccg acgggagaaa ggcttccggc agaactttga
    11521 tgccgccggt ggcgggatac tgaaacgacg gattgtagcc gaaggccttg tccttgattc
    11581 ccaaggcgcc attgacgaca tccttcacat ccggcttggg caccagccac gacacccagt
    11641 cggacgtgag ctcatccagc ggcacctgcc agagcttctc gttaaagggg accatgaaat
    11701 gtttcgcaat accctcgccc aggctctcga cgatccattg tttgaacgac cggctctccg
    11761 ccggagactt ggacgacgga ttcgacagcg tggcgatgaa ccccaagaga cattcccgca
    11821 ccacctccgg cgggaggcca tgcgtattca cctgaaacgg atactcggtg taggtgtcat
    11881 gggaatagac gtaggattta cgggcatgcc gctgaagttg gtccgggagc aggctctcga
    11941 ccagcgcctt gattgcggtc tgccggaagt gaagcagatg ccccgtatag tcgaaggtga
    12001 acccgtcttt cacgtacgaa cggcagagcc ctccgacctc gcgttcccgc tccagaatct
    12061 tgtagggcat cccgcgcagg tgataggccg cgctgagccc cgccaatcca gccccgacga
    12121 tcaaaatcat actacgccgg tcctttctgt cgaacgtgcg gggatcgcct cggtactcac
    12181 ggcgacggct cccgtcgaga cattcacacg cgtcagggcc acggcggcca cggccaggca
    12241 caacaccgtg acaccgctga gcactacggc aagatcctgg gacaccgcca tgaccagcag
    12301 accgatcccc cccaatagcg ccgcgcctag ataactgagc aggacgacct gcgtcaccga
    12361 aagcccccaa tgccgcaaac gaatcgcaag atgatcagga ctgccgagaa acaccggcag
    12421 cccgcgcaag aaccggatgt acatgacgaa cagggtatcg aaaatgggca tgcccaggat
    12481 caacaccggg gtcaacagcg agacggaatg gccggtggtg tatttgccga tcatcgacag
    12541 ggcgcccagc atcagaccga tgaacatcgc gccggaatcg cccatataaa tcgaagccgg
    12601 tcgccagttg tatcgaagga agccgagcag gctccccatg agcgccgcca gcatgaacgc
    12661 gatcgcctga tcgccattca gaatcgccac cacgcacaga aaggcggcac tgatgacgcc
    12721 gacaccggcc gacaacccat ccatgatgtc caataggttg aaggcattga tgatgccgat
    12781 catccagaac accgtcagca cgatatcgac ccaatcgggc aacgacgcga tttcgatgcg
    12841 aatgccgctc ttgatcaaca cgaacaccgc caggaactgg cccgcgagtt tggttcccgg
    12901 cgacaacacc ccgaaatcgt cgatcagccc cagcatgacg atcagtgtcc cggagagcac
    12961 gatgccgagc acgtcctgcc ggaactcgaa ggtaaaggcc aggctcacga gaaacgcgag
    13021 atagatcgcc aatccaccta gataaggaac cggctcacgc tgatgcttca gccggccatc
    13081 cgggttgtcg acgatgccga atttcagggc cgcgcgacgc gcgatcggca cgccgtagat
    13141 tgacagaagc atcgcgacca caaatgtcag cgtgagcaga atcatcgagt tcctgtcgta
    13201 cggtgaccgg acacagggct gtggactgcc cggtgtatct cattcacatg ctcatttccg
    13261 gccaatgtcc ggtacacgtc gccaagccgg gggccccaca ctgccaggga gtactgctcc
    13321 tccacggtcc gacgtccggc ctgccccatt cgagcgcgca actggggctg ccggcagagt
    13381 gtctcgagac gctcatacca ctcctgctcg gtggcggcga gaaatccgtt ctctccgttc
    13441 gcaatgatgt cgcgattcac tccgacgggg gaagccaccg aaggcacccc taccgcaaga
    13501 tactgtagga gcttcaaacc gcacttgccc cgagcccact ccgtgtcctc aagcggcatc
    13561 accccgatcg tggcatcctg cagatccgcc acttcgcgtt gaaagtccca gggcctgaat
    13621 tcaatctcaa cgcccggcat gtgcggcggc cgcgagcaga ccacgcggag ggtcaggcga
    13681 aacctggatt gcaacgcgcg aagggccggc gccagtacat ccagatattt caggttatac
    13741 gccaggccta tccatacgat tgtgatggca tccgagttct gtgcagatga accggtggat
    13801 ctcgtcgaat ccggcttgaa tcgttccgta tcgacgactg tgggaacgac acagacccgc
    13861 ggcgaaaact gcttcgcata ggcggccagc cggtcgttcc cgacgatggc gcctgtggcc
    13921 attgagagga gcgcagggat cttcttctca tgtccaggag tcaggtagat cgcatcatcc
    13981 atttcgatcg ccactcgata tcgacaccag cgcagtagcc gttcagcgag aggaggcgca
    14041 taaggaaaga gctgcccctc gatcacgatc aggtcccgct tgcccagggt caacaacgtc
    14101 cagagtcgtc ggaggaaaca gataagcaca tagggaatct tcagcaaagt ccgtaacgcc
    14161 cgcggatgaa ccttcagaat tgagaagtag gtctctccaa acagagcctc gacgcgacag
    14221 cctattcctg ccgcttgaaa gtgaggcagg aattgaaaga cgcgatagcg actgctgggg
    14281 ccgatgctcg aactcttcga gaaatacgtg accgtcattg cacgtgtttc tgccttgttg
    14341 tgttgaaata tccgccggtt gcgtgcgcac gaccgactgc tcaaatagta ccggagttct
    14401 caggcaagga taggtttttc gttgtttttg acgggggaga gaggcgccag actgggagga
    14461 cacacgggtg ccggagggtt cgcttcgcga cgcttccggc cctcagagct tcaaactcca
    14521 gtcttcccgc aactgggtaa gatttggatt gtagtatggg tcccctttca ttataaactc
    14581 cccccacttc agcctgaatc ttgcctcgtc atccggcgca cgcctcccac gccttgtcgc
    14641 gttctcatgg tgatacagct ctgcttccgg cgtatagacg attcgatacc cttgctgctg
    14701 aatacgaaga cagagatcaa catcgttgaa ttcgacggcc aatcgctcat caaacccgtt
    14761 cacctgtcgg aacacgtctc tcgaaacgag catgcaagcg gcagtgacgg cgctacagtt
    14821 tcgcagcata tgaggcagtc ctctatagtg ttgagtatct ccccgcaggt gacggaacgc
    14881 atgtcccgcc acgcctctga tacctaacac aacccccgca tgttgaatca ttccacttgg
    14941 atacaatagc ttagctccta cagcaccgac ccccgtacgt tgcgcctgcg ccaccatggc
    15001 actcatccac tcgggcgcaa tgacctgggt gtcgtcattc aagaacaaga gaaactctcc
    15061 gttcgctttc gaggccccgt aattgttgat ctcggaaaaa ttaaacggtc ccggaaaccg
    15121 atggatgatc catttttttg agagtgtgct cagatattct ttcgccttag ccgtgcggct
    15181 cccattatcg accacaacga tttcgtagga aggatatcgt gttacggatt caacgctctt
    15241 aatacactgc cgtagcatat cccagcgatc ttttgtggga ataataatag acaccagagg
    15301 gttccttgaa acacggtagt gcaccttgat cttcccggca ccgtcacgca ccacatcagc
    15361 cgattccccg cgacgattca acgcgtcctg aagagatttc atctgagctg tcacatccaa
    15421 ttcagactga ccggcagtga tatcggagga ctcatcagtg atgtggcaat gagacaacac
    15481 ttgaggaacg cgtccgatct tattcgtctc ttccgccacc cgcaatacaa gatgatagaa
    15541 gccatcgttc gcaatgtctc cccaatctgc tccgatatcg aggagaagtt gctttctcat
    15601 gacattgaaa cgaccgacat aattcattcc aatgaggaga tctggactcc aacaaggctt
    15661 aaagaacgga tctagaaatt caccgttctc tcggaccata tcttcatcag cgtagaggaa
    15721 gtccagatcc ggacattctt ggaagcgcgt gatcacagag gacatggcct ctggggcaag
    15781 atagcat
//
LOCUS       DAORQN010000024        15658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_248_length_15658_cov_394.696981, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000024 DAORQN010000000
VERSION     DAORQN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248_length_15658_cov_394.696981"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PK614_03010"
     CDS             complement(join(<1..730,730..988))
                     /locus_tag="PK614_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611071.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB81209.1"
                     /translation="MKQRFSEVQIIGFLREAETGVAVKDLCRRHGFSEASYYLWRNKF
                     GGLEVSDAKRLKALELENARLKRLLAEALLEQAVTREVLRKKLVGAPARREVVRWMCD
                     RGLTERHALQVIGMSASSLRYHPAPDRNADLRQTIVALAHRHRRYGAGMIYLKVRQQG
                     RLVNHKRIERLYAEAQLQVRRRTRKKVPVGERQPLVRPAAANEVWSMDFVFDRSAEGR
                     AIKCLTIVDDATHESVAIVPERAISGQHVTRILDHLAATRGVPQVIRTDNGKEFCGRA
                     MLTWAYAHTVTLRLIEPGKPNQNAYIESFNGRLRDECLNEHWFLTLAHAQAII"
     gene            1059..1235
                     /locus_tag="PK614_03015"
                     /pseudo
     CDS             1059..1235
                     /locus_tag="PK614_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611499.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1327..2453
                     /locus_tag="PK614_03020"
     CDS             join(1327..1582,1582..2453)
                     /locus_tag="PK614_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB81210.1"
                     /translation="MKRTRRNHGAAFKAQVALAACKGDKTLAELAEHFRVHPTQITEW
                     KQQLLTRAADVFGGTKPTSDTPDLKVLHAKIGQLALENDFLGRGAHQGGLAERKAMIN
                     RTHALSVVRQCQILKLSRSTAYYQPTPVSTMELTLMRRIDALHLDYPFAGARMLRDLL
                     RREGQAIGRRHVATLMRRMGITAVYRRPHTSQRHPAHTVYPYLLRHLEITRPNHVWAA
                     DITYIPMARGFVYLCAVLDWASRRVLAWRLSNTLTTDFCLDAVQDAVVRYGTPEIFNT
                     DQGCQFTSLEFTGLLTTHGIQISMDGKGCWRDNVFVERLWKSIKYEEVYLHAYETIGD
                     AHQGVARYLRFYNQTRPHQALDGQTPDQVYCNNLTTRQTAA"
     gene            2516..3423
                     /locus_tag="PK614_03025"
                     /pseudo
     CDS             2516..3423
                     /locus_tag="PK614_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611873.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3444..4667)
                     /locus_tag="PK614_03030"
     CDS             complement(3444..4667)
                     /locus_tag="PK614_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB81211.1"
                     /translation="MIPAVASIFAIAIALRVLGFILLPEPPLPYNALFAYLKGAELLR
                     SGASFGDPFFFFFTATIYSLIFDFVGSVSEASTIGIRIAQMVIDSLTAVLTCLLAREL
                     VSARVATFAGIAWALYPFAIYSTQYVGTEVLFAFFLLAFLLYFVWAIKHESRWLDAGA
                     GLCLGLATLTRGTTQFLPLLLPLVLVWFIKDRRRVLRSSGVMLACFVLVVFPWSLRNY
                     LVLHEVIPIGTNSSVILWGSSKPLLTIDSRVKELPQLYDRARAKGIVAAPPDASPSEK
                     DRYFLQLAIENYRQAVRNDPLGLIELLITKFVRLWYSTESGNNETVILLVNLFIYPLA
                     LVGAVAVWKERNPFGLLLFGVVIYFALLHWATLPLFRYILPVMPIILCLACLGFDRAF
                     SVTRVVDLLPRRGSL"
     gene            complement(4664..5770)
                     /locus_tag="PK614_03035"
     CDS             complement(4664..5770)
                     /locus_tag="PK614_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB81212.1"
                     /translation="METKTRTTHFLILIPAHNEEDGLPETLQSLSALQYPKDLMHIVV
                     IADRCADDTARVARAGGAQCLERNEGPGGKGAAMSWAMQVLRKDGVVFDALVIVDADC
                     LADSRLLEAFDDALAKGHEVQQGYNYLSNPWETPFTRVIAVTSVLRNFLFYGGKEAIG
                     CSAMLSGTGMCLSRSVVDQHGWSAFSVAEDWEFSVSLLLNDVVIHFNPLARVFARESK
                     GLKQASRQRLRWATGKYAVMTNGARRLFLQGLSRGKANLIDGAVTLAAPNYSSQASMT
                     LFALVCSLFLSQEPAWGFLLPWSLAVLGSLGGYFLLGAIHTQSPLKTLAGIPYIAVFL
                     PWRLSIEILGMLGFGRKGWGRTFRDSASRPTEGK"
     gene            complement(5910..7064)
                     /locus_tag="PK614_03040"
     CDS             complement(5910..7064)
                     /locus_tag="PK614_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81213.1"
                     /translation="MITKVCHIAVADLWGGAEVHLVTLLLSLSKIQDLEMSVVLFTEG
                     RVAQELRAAGLEVVVIAEHEHNLFVILWKLVKHFRKHQFMVVHAHKPKDNFLGGVACR
                     LAGVPYLVRTLHGSWEPFSGFQHFKIKVYEHIDRLANKYLVSAIIAVTLKIQSLLTQG
                     YSREKVVCIHNGIDVERLMLSRGRRSVRKELNINERALLLGVVGRLTAVKGHIHLLKA
                     MQILVSKNSGTHLIIVGDGPLRKQLEDIVVEFKIQKNVVFVGHQSDVSDFIEAMDIFV
                     LPSLHEGIPMVLLEAMALSRPVVASKTGGVPEVVDDGENGLLVEPGNHEAIAEAIESL
                     IKQVGKAGQLGRNGRVRVSQNFTASTMAKKTANLYRRLVGSRFANSAAQA"
     gene            complement(7105..7866)
                     /locus_tag="PK614_03045"
     CDS             complement(7105..7866)
                     /locus_tag="PK614_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRB81214.1"
                     /translation="MRVIINSDDLGMSPVVNEKILDLMSRRRITSASILANGPSVEQA
                     IRLIPKGTGCSLGVHLNLTEFEPLTPARHLGGLRAYLNENGAFAGERNMSNLSISPSL
                     REGIYRELRLQVEKVVALGVKISHLDSHNHVHTIPHVFPILKRLQREFGIRKVRTTWN
                     IYPPQKPVSMALRIRKKVWDFSLRRLYRTRTTQGLTTFAIFYDLARKEELSCSSIELM
                     THPGHAEYEEETRLLDRDWESEILFPVDLISYHAL"
     gene            complement(7904..9730)
                     /locus_tag="PK614_03050"
     CDS             complement(7904..9730)
                     /locus_tag="PK614_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HRB81215.1"
                     /translation="MFEFHQYDPLLHTKPLQPFSPYHPSDNVAKMSLLFWSEHCIECA
                     APACFSTCDLYDHRPDSRCRRFTFGMFRNTKFASIRGYGAEIEFKKWGKLEARANLST
                     TPISFLLRQERLIEFFLPVVNLVGSALMRVTRDERWSYLSYAILARVTKCLAHLSKRD
                     GQPDAFLFEVYNPSDTAVKIQVSMTPAKLSDRSALIELSRPGSHFAATITLEPGYSRH
                     QIDRPLFNALLESGVPFDVSLLPEGDNALRIIVLVADFVVFKDTVEKKTKTGQPDVKC
                     IVWDLDNTLWEGVLVEDSNVRLRLSALDLIKQLDQKGIVMSLVSKNDAEKALEKLKEF
                     GIDEYFLYPQINWLPKSQNIQQIAKCLNIGLDTFAFIDDNPFELAEVGETLPMVRCIS
                     VDHLSSVVDDPRFAGSDSPDAKNRRQYYQTAAMREQTQASFGDNYIDFLAHSNIKLTI
                     KKYESGDFERVAELVQRTNQLNFSGKKYKREEIADLVRRPEIDKYLLACTDKFGSYGT
                     VGFSIVKQAPGEIEVKDFMLSCRVQGKFIEKAFFGFLLKRQDHESGPRFRVGFMQTSR
                     NTPAKQVLDKLNFVSAEDGVGLVLDRPGESLACDFIRVEYQD"
     gene            complement(10267..11376)
                     /locus_tag="PK614_03055"
     CDS             complement(10267..11376)
                     /locus_tag="PK614_03055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81216.1"
                     /translation="MIQTILFLSTSSGPGGAERVISSLAASLDPSRYRAVLCLFRQGW
                     IQERSESRGVRTHIIPTRGMTDWRWALRFRRLLEEEHVDLIHAHEFDANVQGAFVAAM
                     SGTPLVATVHGKHYFWEKLRRRLAYRWVSRRATMVAVSEDLKKFIVEKVGVCPERITV
                     LYNGVDVPPPPTQFDVDACRREFGLPEKDQIVGVVGNLYPVKGHQYLIEGIPAVLKKC
                     PDTSFIFAGRGQLEMELKNQVHRLGLSERVHFLGLRQDIQAILAMLDVFVLPSLSEGL
                     SMAILEAMMAKKPVIATRVGGNPEIVLDGETGFLVPPKDSQALAASLATLLLNRDQAT
                     QFAERGKRRAEGLFSLQTMVHAYQSLYDLCLRSSP"
     gene            complement(11467..12768)
                     /locus_tag="PK614_03060"
     CDS             complement(11467..12768)
                     /locus_tag="PK614_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB81217.1"
                     /translation="MQKESNVGFYLVMLAMLFEFGRPQDVLPPLKAIPIPTLLDVSVL
                     IAVLVSGKATFANLQTRLWMGLLVFMAMWVPFANNNFWAFITFKEMTLYFFFYLGIVT
                     FVNTTSRMRKLIFMWLGVHAVLGINGILHHGQGIGGWLGDENDFGMEMNVAVPVAFFM
                     YQASTNQRSKLLYMILLGLFVMSVVATSSRGAFLGLLALGTYCWLYSPRKIMSLVLGV
                     GLVGLVLVAAPQEYWDRISSITDDSTMETGTAGQRMFTWGIGWEIFTANPIFGIGQGN
                     FPWTIGEYMGGRTWQTKSLAGRQAHSLYFTLLPELGLVGVLIFGTLVYLNYRDTRVNQ
                     FLPVAVGGTDGRKQERDPQVAQAVLFGNAILGGMIGYLTTSAFISTLYYPTFWILMGL
                     AVALRNSTQAYTVSQPDTSATSTVTPKVSLWGRPRPVRLRS"
     gene            complement(12880..13626)
                     /locus_tag="PK614_03065"
     CDS             complement(12880..13626)
                     /locus_tag="PK614_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB81218.1"
                     /translation="MSLRTRLFNIYWTLRGAIVPKLRYSQYSYEESLKRYVTPSVEWV
                     EIGCGHSILPSWRANEERQLVRTCKAIFGIDYDLPSLKAHPNIAKKLRGDVTKLPFRN
                     EAFDLVTANMVVEHLDNPDEQFREIGRILKSKGVFLFHTPNAYGYGVILSKVVPEWLK
                     GKLIYLLEGREEHDVFPTHYKANTEAQVKALAQANGFEVLQLDLIPTDAIFAMFPPLA
                     ALELLWIRLLMTPPFRSLRTNMIVALRKSA"
     gene            complement(13692..14234)
                     /locus_tag="PK614_03070"
     CDS             complement(13692..14234)
                     /locus_tag="PK614_03070"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRB81219.1"
                     /translation="MKRAVNEALDQWRYRSLVNRQAFPSPVRVILVVCKGNICRSPLA
                     EAYLKHRVEKHGLPIVVESAGLETSFGKAAHPLAQVVGTQGGLLLTKHATQPLHKEQV
                     DRADMILVMEWRQRRRVLKLYPQAKQKVFLLRQFYDQSVREVADPYSGTLEDFQTCFS
                     MIKQACDVLVMQMLPSGKQA"
     gene            complement(14289..15476)
                     /locus_tag="PK614_03075"
     CDS             complement(14289..15476)
                     /locus_tag="PK614_03075"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRB81220.1"
                     /translation="MKVLITDGNERAALAVTRALGGEQVEVIVGAESQRSLAGSSRYC
                     RESIAYPSPYQEPERFIATLMEAVRTHRVDALIPLSDIAMHVLGPEKAQFEKYTHFPA
                     PSPQAFQEISDKYRLMQQAITEGVAIPDTIFVPEGRVERVVQGIVDFPVVVKPGCSLV
                     KDGNRWTKTSVCYAESPEELLSLYRDKPYLRQPSLIQRRVIGEGQGLFVLMQEGIPLG
                     MFAHRRVRERPPSGGVSVLRESIALPKSMVEATLKLLQRVKWHGVAMVEFKVDAATQR
                     PLLMEINGRFWGSLQLAVDAGVNFPLHLLNMAMGACETIPENGYRVGVKSRWLLGDLD
                     QLVMRIRKSDRALNLPPGAPSRLQAVLSFCRFFERNTFYEVERIDDLGPSRFEVMRYF
                     KLA"
BASE COUNT         3963 a         4463 c         3811 g         3421 t
ORIGIN      
        1 tcaatgatgg cttgggcatg cgcgagggtg aggaaccaat gctcgttcag gcactcatca
       61 cgtaggcgcc cgttgaatga ctcgatgtag gcattctggt tgggcttgcc cggttcaatc
      121 aggcgcagcg tcacggtgtg cgcgtaggcc cacgtcagca tggcacgacc acaaaattct
      181 ttcccattgt cggttcgaat cacctgaggc accccgcggg tcgcggccaa gtgatccagg
      241 atccgcgtga cgtgctgacc gctgatggcc cgttccggca caatggcgac cgactcatgc
      301 gtggcatcgt ccacgatcgt caaacatttg atggcccggc cttccgcact gcggtcaaag
      361 acgaaatcca tggaccagac ttcgttcgcg gccgcaggcc ttaccagtgg ctgacgctca
      421 cctaccggca ccttcttgcg ggtgcggcgc cgcacctgca actgggcttc ggcatagagc
      481 cgttcaatcc gcttgtgatt caccaatcga ccctgctgac gcaccttcag ataaatcatg
      541 ccggccccgt accgccgatg gcgatgcgcg agtgcgacaa tggtctgtcg cagatcggca
      601 ttccggtccg gcgcggggtg ataccgcaag ctgctggcac tcatgccgat cacctgcaac
      661 gcatggcgct ccgtcaggcc tcggtcacac atccagcgca ccacctcgcg acgagctggt
      721 gcgcctacca cttttttcgg agcacctctc gagtcacggc ctgctccagc agtgcctccg
      781 ccagcaaccg cttcagccgg gcattctcca gttccaacgc cttcagccgc ttggcgtccg
      841 acacctccag gccaccgaac ttgttccgcc agaggtagta gctcgcttct gagaatccat
      901 gccgccgaca cagatccttc acggccaccc ccgtctccgc ctctcgcaaa aacccaataa
      961 tctgtacctc tgaaaaccgc tgcttcatat ccaatctcct tcctagtggg ggattggact
     1021 cgaaatcgcg gcgctactca agacgggggt gacgtcggat cctcaaagaa gccgatgccg
     1081 ggcgcccggt caacgagctg tggcggacac acggcatcag ttccgcgacc tactacaagt
     1141 ggaaagccaa gtacgggggg ctcgaggcct cagatgtgaa gcggctgaag gaattagagc
     1201 aggagaacaa ccgtcttaag cggatgtatg cggacagaga gggctccaga aattcggaca
     1261 gtgtgttaag tggtagcctg gcttcaccaa ggccaccagt tggtggcaaa ccagaggagc
     1321 gaggcgatga agagaacaag acgcaatcac ggggcggcat tcaaggccca ggtggccttg
     1381 gcggcgtgca agggcgacaa gacgctggcc gaattagccg agcactttcg cgtccatccc
     1441 actcagatca ccgaatggaa gcagcaatta ctgacgcggg ccgcggacgt atttggcggc
     1501 actaaaccaa cgtccgacac gccggatctc aaggtcctcc acgcgaagat cgggcaactg
     1561 gcgctggaga atgatttttt agaaggggcg ctcatcaagg cgggcttgct gagcgcaaag
     1621 cgatgatcaa ccgaactcac gccctgtcag tcgttcggca atgtcagatc ctgaagctct
     1681 cgcggtcgac cgcgtactac cagccgacgc cggtgtcaac catggagctg acactgatgc
     1741 gccggattga cgcactgcat ctggactatc cgtttgccgg tgcccggatg ctgcgtgacc
     1801 tcttgcggcg agagggccag gccattgggc ggcggcacgt ggccaccctg atgcgacgca
     1861 tgggcatcac ggccgtgtat cgcaggcccc acaccagcca acggcatccc gcccatacgg
     1921 tgtacccgta tctcctgcgc cacctggaga tcacccgccc gaatcacgtc tgggcagcgg
     1981 atatcaccta cattccgatg gcgcggggct tcgtgtattt gtgcgcggtg ctcgactggg
     2041 ccagtcgccg cgtgttggcg tggcggttgt ctaacacact gaccaccgat ttctgtctcg
     2101 acgcggtgca ggacgcggta gtccgctatg gcacaccgga gatctttaac accgatcaag
     2161 gctgtcagtt cacgagtctg gagttcacgg ggctcctgac cacccacggc atccagatca
     2221 gcatggacgg gaaagggtgt tggcgcgata acgtgttcgt ggaacgactc tggaaaagca
     2281 ttaaatacga ggaggtctat ctgcacgcct acgagaccat cggcgacgcc caccagggcg
     2341 tagcgcgcta tctgaggttc tacaaccaga cccgaccgca tcaggcgctt gacggccaga
     2401 cacccgacca ggtgtactgt aacaatctga caacacggca gacagctgcg tagtcagcaa
     2461 tccgccaggc accacttaag gaatgctcta cactgtccaa ccaaccggag ccacctctga
     2521 cttgtcgctt gagaatgcgg cgctgaaaga tgtcatcgca aaaaagttct gaagcccact
     2581 gaacgtcggg aagtcgtcac gcatctcgtg accgtacacg gcctcccggt tcagcgggcc
     2641 tgtcgcgcag tgggtttggg acgggcgacg tactaccggc cgctcatgga ctgggctcgg
     2701 cgggatgcgc cggtgatcgc ggcgttgacg acactcgtcg cggccaagag tcgctggggc
     2761 ttttggaagt gttgggggcg gctccgattg gatggtcatc cctggaatca caagcggctg
     2821 tggcgggtat actgtcaact ccgcttgaat ctcccccgtc ggacgaagaa gcggctctcg
     2881 gctcgtctgc gacagccgtt gggcgtgatt ccgtacccga acgccgtgtg ggctgtggac
     2941 ttcatgagtg acaccctgta cgggggccga cgattccgga cgttgaacgt gctggatgaa
     3001 ggcgtgcggg aaggcttagc cattgagatc gatacgtcgc tcccggccga gcgcgtgatt
     3061 cgcgtgctgg agcaagtcgt gagctggcgg gggcagcccc aggcgattcg gctcgacaat
     3121 ggccccgaat tcctcgccga ccggttcgtc acctggtgcg cggagcgggc gattcagctg
     3181 tggcacatcc aaccgggcaa gccggatcag aatgccttga ttgagcggta taaccggacc
     3241 tatcggaccg aagtgctcaa tgcgtatgta ttcgagtcgt tggatcaggt gcgagagatt
     3301 agtgccgaat ggttgcagag ttacaatgaa gagcggcccc atgacgcgtt ggcgggcctg
     3361 ccgcccgcca cgtatcgggc ccaagtggaa gccagaaact ctcccatgaa actgtctcct
     3421 tgacggggga gcttacggag gctctagagt gaacccctac ggggaaggag atctacaacc
     3481 ctggtgacgc tgaatgcgcg atcaaaaccc aaacatgcaa gacagagaat gatgggcatt
     3541 accggcagta tgtagcggaa cagcgggagc gttgcccaat gcaataacgc gaagtatatc
     3601 actacaccga aaagcaggag cccgaaaggg ttccgctcct tccagaccgc gacggcgccg
     3661 accaatgcca ggggatagat aaaaagattt acgaggagga taacggtttc attgttaccg
     3721 gattcggtcg aataccatag acgaacaaat ttcgttatca atagttcgat aaggccaagc
     3781 gggtcattgc ggactgcttg tcgataattt tcaatggcaa gttgcagaaa ataccggtct
     3841 ttctcggatg ggctggcgtc gggaggcgcc gcgacgattc ccttggcccg ggcccgatcg
     3901 tacaactgcg gcaactcctt caccctcgaa tcgatcgtga gaagcggctt actcgacccc
     3961 cacaggatga cgctgctgtt cgtaccgatc ggtatgactt cgtgcagcac cagatagttc
     4021 cgcaggctcc aggggaaaac cacgagcacg aaacaggcca gcatcacacc actactccgc
     4081 aagactcgcc ggcgatcttt tatgaaccag accaacacga gagggagaag tagcggcagg
     4141 aattgcgttg tcccacgcgt caaggttgcc agacccagac agaggcctgc cccggcatcc
     4201 agccatctac tctcgtgttt gatggcccac acgaagtaca gcagaaaggc caggaggaaa
     4261 aatgcgaata gcacctccgt ccctacgtac tgagtcgaat agatggcaaa tggatacagc
     4321 gcccatgcga tgccagcaaa ggtggcgacc ctagcgctca ctagctccct ggccagcagg
     4381 cacgtcaaga ccgcagtcag ggaatcgatt accatttgag ctatccggat cccgatcgtg
     4441 gatgcttcgg acacgctgcc gacgaaatca aagatcaatg aatagatcgt agccgtaaaa
     4501 aaaaaaaaaa acggatcgcc gaagcttgcg ccgctccgga gaagctccgc ccctttcaga
     4561 tatgcaaaca gtgcattgta cgggagagga ggctccggca agagaataaa ccccagcaca
     4621 cggagggcaa tggcgattgc aaatatactt gcgacggctg gaatcatttc ccctccgtcg
     4681 gccgcgaagc ggaatctcga aaggttctcc cccaaccttt tcttccgaat cccagcatgc
     4741 caaggatctc aattgaaagt cgccagggaa ggaacaccgc gatataggga attccagcta
     4801 acgtttttaa cggggactgc gtatgtatcg cgcccaaaag gaagtacccc cccaatgatc
     4861 caagcactgc cagcgaccac ggaagaagaa atccccatgc tggctcctga ctcaggaaga
     4921 ggctgcagac aagggcgaag agggtcatgg aggcctgact cgaatagttt ggagcggcca
     4981 aggtcacagc cccgtcaatt aaattggctt tcccgcgcga caaaccctgc aagaaaagac
     5041 gtctcgcccc gtttgtcatc accgcatact tgcccgtcgc ccatcgcaat ctctggcgcg
     5101 aagcctgctt gagcccttta gactccctgg caaagacacg agccaagggg ttgaaatgaa
     5161 tcacgacgtc atttagcagc agtgaaaccg aaaactccca atcttccgct accgagaacg
     5221 ccgaccaccc atgctgatcc acaacggatc gactcaagca catccccgtt ccgctcaaca
     5281 ttgcggaaca gccgattgcc tcctttcctc cataaaacag gaagtttcgc aggacactcg
     5341 tgaccgcaat cacacgggta aacggagttt cccatggatt ggagaggtaa ttgtacccct
     5401 gctggacttc atgccctttt gcaagagcat cgtcaaacgc ctctaacaac ctggaatctg
     5461 caagacagtc tgcgtcaacg atgacgagcg cgtcgaacac cacaccgtcc ttccgtaaca
     5521 cctgcatcgc ccaggacatg gccgcaccct ttccaccagg cccttcattg cgttccagac
     5581 actgagcacc acccgcccgc gcgacccttg ccgtatcgtc agcacagcga tccgcaatca
     5641 ccacgatatg catgaggtcc tttggatatt gcagggcgga cagactctgt agagtctccg
     5701 gcaatccatc ttcttcattg tgagccggaa taagaatcag aaaatgagtc gtgcgagtct
     5761 ttgtttccat cgatggagaa gtccggtgcg agatagcaag aattgcgtgc ccccactggt
     5821 agaatgaaat gagagaaaga agtactaaaa caagaaaaca gactatacct agtatcagta
     5881 acatcgtagc caacttttac tttagagggt taagcctgag cggcactgtt cgcaaaccta
     5941 gagccaacca atcgacgata cagattcgct gtttttttcg ccattgtgct tgcagtgaaa
     6001 ttttgagaaa cccgcacacg cccattcctc cctagttggc ccgcttttcc cacctgcttg
     6061 ataagtgact caatagcctc agcaatcgcc tcgtgatttc caggctcaac caacaaccca
     6121 ttctcaccat cgtctacaac ttcaggaact ccacctgttt tactagctac gaccggacga
     6181 ctcaatgcca ttgcctcaag caataccatt ggtatcccct cgtgcaggga ggggaggaca
     6241 aagatatcca ttgcctcgat gaaatcgctt acatcactct gatggccgac aaaaacgacg
     6301 ttcttctgaa ttttaaactc tacaacaata tcctccaact gctttcgcag tggaccatct
     6361 cccacaataa taagatgggt tccgctgttt ttacttacaa gaatctgcat agccttcagc
     6421 aagtgaatat gtccctttac cgctgtcagc cttccaacaa cacctaacaa caacgccctc
     6481 tcgttgatat tcagttcttt tcgaacgctc cgccttcccc tgctgagcat aagcctttcc
     6541 acatctattc cgttatggat acataccacc ttctctcttg aatagccttg ggtaaggagg
     6601 gattgaatct ttaaggttac ggcaattatg gcgctgacca aatatttatt agcaagccga
     6661 tcgatatgtt catagacttt gatcttgaaa tgctgaaagc ctgaaaatgg ctcccacgac
     6721 ccatgaagcg tcctgacaag gtacggtaca cctgccaacc tgcaggccac cccacctagg
     6781 aaattatctt tgggtttgtg ggcatgcaca accatgaact gatgtttccg aaaatgcttt
     6841 acgagcttcc acagaataac gaacaagttg tgttcatgct cagcgataac cactacttcc
     6901 agccccgcag ctctcaattc ctgcgcaacc ctcccctcgg tgaaaaggac caccgacatc
     6961 tccaaatcct gtatcttgga tagcgatagc agtagagtta ccagatgaac ctctgccccc
     7021 ccccaaagat cagccaccgc aatatggcac accttggtga tcatggatgc ccgtgcactt
     7081 gacagcttac gaggcccaga agaactagag cgcatggtag cttatgagat cgacaggaaa
     7141 aagaatctcg ctctcccaat cgcggtctaa gagccgggtc tcttcctcat attcggcatg
     7201 tccagggtga gtcataagtt caatcgaact acacgacaat tcttccttcc tggccaaatc
     7261 ataaaaaatc gcgaaagtcg ttagtccttg agtagttctc gtacgataga ggcggcgcag
     7321 ggaaaaatcc cagacttttt tccttattcg aagtgccata gaaacaggtt tctgaggagg
     7381 gtaaatattc caggtcgtac gcactttgcg aatcccaaat tctctctgca ggcgtttaag
     7441 tattgggaac acatgaggga tagtgtgtac atggttgtgc gagtcgagat gcgatatctt
     7501 gacgccaaga gcgactacct tctccacctg caagcgaagc tctctgtata ttccttccct
     7561 tagagaaggt gatatggaca agttgctcat gttccgctcc cccgcgaatg caccattctc
     7621 attgaggtag gcacgtaggc cgcctagatg tctggcagga gttagtggct cgaattcggt
     7681 aaggttgagg tgaacaccga gagagcatcc tgttccctta ggaatcagcc gtatagcctg
     7741 ctcgactgaa ggaccattgg caagaatgct tgccgaggta atccgtcgtc gggacatgag
     7801 atccaggatc ttttcattca ccacaggact catgcccaga tcgtcgctat ttatgattac
     7861 cctcatacat ggaccatcac ttggcgtcat agcttgtccc tcgctagtcc tgatattcta
     7921 cacggatgaa atcacaggca agggactcgc cagggcgatc aagcaccaac cccacaccat
     7981 cttctgcgct aacgaaattg agcttatcca acacctgctt cgcaggagta ttccggctgg
     8041 tttgcatgaa gccgacccga aaacgaggcc cggactcatg atcctgccgc ttcagtagaa
     8101 agccgaagaa tgctttttca atgaactttc cttggactct acaggacaac atgaaatcct
     8161 taacctcaat ttctccaggg gcctgcttca caatgctaaa acctacagtg ccgtacgagc
     8221 caaatttgtc ggtacacgct agtaggtatt tatcaatctc tggcctcctg acgagatcag
     8281 caatctcctc tctcttgtac ttcttgccgg aaaaatttag ctggttggtg cgctgcacaa
     8341 gttcagcgac tcgttcaaaa tcgccggatt catatttttt tatagtcagt ttaatattgc
     8401 tgtgtgcgag aaaatcgatg tagttatccc caaaagatgc ctgcgtctgt tccctcatcg
     8461 ccgcagtctg ataatactga cgccgatttt tcgcatctgg tgagtcgctt ccagcaaaac
     8521 gcggatcatc tacgacggag ctcagatggt caactgaaat acaccgcacc ataggtagcg
     8581 tctcaccaac ttctgccaat tcaaatggat tatcatcaat aaacgcaaaa gtgtccaagc
     8641 caatattaag gcatttggca atctgctgta tgttttgact tttcggcaac cagttgatct
     8701 ggggatataa gaagtactca tcaattccaa actcctttag cttttcaaga gccttttctg
     8761 catcgttctt gctgacgagg ctcataacaa tacctttctg atcaagctgc ttgatcaagt
     8821 caagagcact caatctcagc cgcacattgg aatcctcaac taataccccc tcccaaagcg
     8881 tattatccag atcccaaacg atacacttaa catcaggttg ccctgttttc gtcttcttct
     8941 caaccgtgtc tttaaataca acgaaatcgg caaccagaac tattattctg agcgcattgt
     9001 ctccctctgg gagaagagat acatcaaaag gcacgcctga ttcgaggagg gcattgaaga
     9061 ggggacgatc aatctggtgc ctggaataac ccggttctaa agtaatcgta gctgcaaaat
     9121 ggctacctgg ccggctgagt tcgataaggg cactcctgtc cgacagtttt gcaggcgtca
     9181 tactcacctg tatcttcacg gctgtatcag aagggttgta cacctcaaaa agaaatgcat
     9241 cgggctgacc atctctttta gagagatgag ccaaacattt ggtgactctt gcgagaatcg
     9301 cataggagag ataactccac cgttcatccc gagtgacccg cattaaggcg gagccgacaa
     9361 gattaaccac tggcaagaag aactcaatca agcgctcttg acgcagaagg aaacttatcg
     9421 gcgtcgtgga aaggttggcc ctggcttcaa gtttgcccca tttcttaaat tctatctcgg
     9481 cgccgtaccc acggatggat gcaaactttg tattcctaaa cattccaaaa gtgaatcgac
     9541 gacaacgact gtctggccta tgatcataaa ggtcgcaggt gctgaaacaa gcgggtgcag
     9601 cacattcaat acaatgctca ctccagaaca aaagagacat ttttgcgaca ttatcacttg
     9661 gatgataggg ggaaaatggc tgaaggggct tcgtatgaag caatgggtca tactggtgaa
     9721 attcaaacat ggctctttcc tctttcgttc atcacgaaac gaccacgcct actgccatag
     9781 ttatctctta acttggtccc cttgaggata acctcgcacc tatcccatgc ccggaggttg
     9841 aagcccttta caatgcctgc tatttcatcc ccttggtatg catacaaaaa tactctccgg
     9901 caccttcccc actcgcaaac atttccggct gtacaaatag aaggcttcac ggcaacacta
     9961 tcaaaaacca ttcatgcaga aatccgaaga taacgatccc cactacatcg ataagctaac
    10021 ggagcgggtt acagagcagt cagatgtcag atcgaatcta ccgattcatt tattaacgca
    10081 gtactaaagg aggaccgaac ttcaaattat tgaaccggct gtttcaaaat ttcttctgcc
    10141 tacttgccct catcgaacaa gacgacggaa ctgtcgaact cctttcaacg caggtacatc
    10201 aatgcatgac gcctgacttc tgacatcatg actcccacac actctctcac ttgctcccat
    10261 acaaaatcac ggacttgacc gcaggcacag gtcgtagagc gattggtacg catgcaccat
    10321 ggtctgcaaa ctaaacagac cttcagcccg acgtttaccc cgctctgcaa attgtgtggc
    10381 ctggtctcta ttcagcaaca atgtggcgag actcgccgcc aaggcttgac tatccttggg
    10441 cgggacaaga aaaccagtct cgccgtcgag cacaatttca ggattcccgc ctacacgtgt
    10501 agcgatcaca ggcttttttg ccatcatcgc ctcaagtatc gccatcgaga gtccttctga
    10561 taacgacgga agaacgaaaa cgtccaacat ggcaagaatc gcctgaatat cctgccgcaa
    10621 gccaagaaaa tggactcgct cgctcaaacc aagccgatgg acttgattct tcagctccat
    10681 ctcaagctgg cccctcccgg caaagatgaa ggaggtgtcg ggacatttct tcagcaccgc
    10741 aggaataccc tcgatcaaat attgatgtcc cttcacgggg tataaattcc ccacgacacc
    10801 aacaatctga tccttctctg gcagaccaaa ctcccttcgg cacgcatcaa catcaaactg
    10861 cgtaggaggt gggggcacat ctaccccgtt atagaggact gtaattcgct ctggacaaac
    10921 cccgaccttc tccacaataa actttttcaa atcctcagaa accgccacca tcgtcgctcg
    10981 ccgactgacc caccgatagg caagtcggcg cctgagcttt tcccagaaat aatgttttcc
    11041 gtgaaccgtc gccacgagcg gagtcccgga catggccgcg acgaacgccc cctgcacatt
    11101 ggcatcaaac tcatgcgcgt gaataagatc gacatgctcc tcttccaaga gccgcctaaa
    11161 tcgaagcgcc caccgccagt ctgtcatccc acgcgtggga ataatatggg tgcgaactcc
    11221 cctactttca ctccgttctt gtatccatcc ctgacgaaac aaacaaagaa cggcccgata
    11281 tcgagaagga tccaaagatg cggccaggct gcttatgact cgctcggctc ccccgggtcc
    11341 gctgctggtg gaaagaaaca gaattgtttg aatcattttt ttcaatatac ccgacatgct
    11401 ctgctcatcg catttgcaca gagagaaaca cccagtctct ttgcatcacc ccacacagcg
    11461 cgacgatcag gatcgtaatc tcacgggtct gggccgcccc caaagagaca ctttcggtgt
    11521 gacagttgag gtagcactag tgtcgggctg gctcacagta taggcctgag ttgaatttcg
    11581 aagggcgaca gccagtccca tcaggatcca gaaggttggg tagtacaaag tggagatgaa
    11641 ggcgctcgtc gtgagatagc ctatcatccc gcctaaaatc gcattcccga acaggacggc
    11701 ttgggcaact tgagggtccc gctcctgctt ccgtccatcc gtaccgccta ctgcgacagg
    11761 caagaactga ttcaccctgg tatctcggta gttgagatat accagcgtac caaaaataag
    11821 tacgccgaca agtccgagct cgggtagcaa ggtaaagtac agggaatgcg cctggcggcc
    11881 ggccaatgat ttggtttgcc aagtgcgccc ccccatatac tcgccgattg tccaaggaaa
    11941 gttgccctga ccgatcccga aaattggatt ggcggtgaat atttcccacc ctatccccca
    12001 ggtgaacatc cgctgtcctg ccgtaccggt ttccatcgtg ctgtcgtcgg taatagaact
    12061 gatacgatcc caatattcct gtggtgcggc aacaagtacc aggccaacaa gccctacgcc
    12121 caagacaagc gacatgattt ttctgggaga gtacaaccag caatatgtcc ccaaggccaa
    12181 taaacccagg aaggcacctc gcgaagatgt ggccaccacc gacatgacaa agagacctaa
    12241 caaaatcata tacagtagct tcgaccgctg attcgtcgac gcctggtaca tgaagaaggc
    12301 gaccggaact gccacattca tctccatgcc gaaatcattt tcgtccccta gccaaccacc
    12361 aataccttgc ccatgatgta aaattccatt tattccaagg acggcgtgca cacccaacca
    12421 catgaatatg agtttccgca ttcgactagt ggtattcaca aaagtcacga tgcccaaata
    12481 gaaaaagaaa tatagcgtca tctccttgaa cgtgatgaac gcccaaaagt tgttattggc
    12541 aaatggaacc cacatggcca tgaatacaag cagtcccatc cacaaccttg tttgcaagtt
    12601 ggcaaacgtg gctttgccgg aaaccaagac cgcaataagc acggacacgt ctagcagagt
    12661 tggaatcgga attgctttga gcggagggag gacatcctgc ggccggccaa attcaaaaag
    12721 catggccagc atgacgagat agaaaccgac gttggactct ttctgcaccg gcaacgctcg
    12781 gactgtggcc ccagattgag gtcttccggc catctgttga ttggactgta tcattgatta
    12841 aaggtctcga tcacaacgct cgaacctgtc tgtgcgacgc tatgccgatt tcctgagtgc
    12901 tacgatcata ttggtgcgaa gagaacgaaa cggaggggtc atgagcagcc gtatccacaa
    12961 taattccagg gcagccagcg gtggaaacat ggcaaagata gcgtcggttg gaatcaaatc
    13021 aagctgaagc acctcaaatc cattggcctg agcgagcgct ttaacttgag cctccgtatt
    13081 agccttgtag tgggtaggaa aaacatcgtg ttcttctcgc ccttcaagca aataaatgag
    13141 cttgcccttg agccactcgg gtacgacttt ggagaggatg actccatatc cataagcatt
    13201 cggggtgtga aacaagaaca cgcctttcga cttaaggatg cgcccaatct ccctaaactg
    13261 ttcgtctgga ttgtcaagat gctcgaccac catatttgcg gttaccaggt cgaacgcttc
    13321 gtttctaaag ggtaacttgg tgacgtcacc gcgcagcttt ttggcgatgt tgggatgcgc
    13381 tttgagggat ggaagatcat aatctatgcc aaatatcgcc ttacaggttc ttaccaactg
    13441 gcgctcttca ttcgctcgcc agctgggaag gatggagtgt ccacagccta tctccaccca
    13501 ttcaactgat ggcgtcacat accgcttcag cgattcctcg tacgagtact gggaatatct
    13561 cagcttcggc acaatggcgc cccgcagcgt ccaatagatg ttgaacagtc ttgttcgcaa
    13621 actcatgtcg atgccgggcc tcccttgatc cttgccatct gcactcgcgt ttcgatcaaa
    13681 tcaattcaat cctatgcttg cttgcccgag ggcaacatct gcatcaccaa tacatcacag
    13741 gcctgcttaa tcatcgagaa gcaggtctga aaatcttcca gcgttccact gtaggggtca
    13801 gcaacctctc gcactgactg atcgtaaaac tggcgaagca aaaacacttt ctgcttggcc
    13861 tgagggtata gcttgagcac gcggcgtcgc tgccgccact ccatgaccaa aatcatgtcc
    13921 gcccgatcaa cctgttcttt gtgcagtggt tgtgttgcat gcttggtgag taaaagcccc
    13981 ccctgcgtgc cgacgacttg cgcgagcgga tgcgccgcct ttccaaacga tgtttcaagc
    14041 ccagccgatt caaccacaat cggcaaccca tgcttttcaa cccgatgctt gagataggct
    14101 tctgctaacg gactgcgaca gatattgcct ttacagacaa ccaagatgac acgcacggga
    14161 ctcggaaaag cctgccgatt aaccaggctc cgatagcgcc actgatctaa cgcctcgttc
    14221 acagccctct tcatcgaacg agcaactcct cgcagggagg cagaatcaac ttgggacatc
    14281 acaactccct atgccagctt aaaataccgc atgacctcaa accggctagg cccgagatca
    14341 tcaatacgct caacttcata aaatgtattt cgttcaaaaa accggcagaa ggacaggact
    14401 gcttgaagcc tggatggggc gccaggcgga agattcaacg cacgatcact cttcctgatg
    14461 cgcatgacta attgatccaa atcaccaagc aaccacctgg acttgactcc gacacgatat
    14521 ccattttcag gtatcgtctc gcacgctccc attgccatat tgagtaaatg aaggggaaaa
    14581 ttgacgcccg catccacagc cagctggagc gatccccaaa accgcccatt aatttccata
    14641 agcaagggac gctgagtggc ggcatctact ttgaattcca ccatggccac accatgccac
    14701 ttcacacgct gtaacaactt cagggtagcc tcaaccatgg atttcggcaa ggcaatactc
    14761 tcgcgcaata cgctgactcc gccggaggga ggtcgttcac gcactcgcct gtgagcaaac
    14821 atccccaacg gtatcccttc ctgcatcaac acaaacaggc cctggccctc accgatcaca
    14881 cgccgctgga tcaacgaggg ctgcctcaga taaggcttat cacgatagag gctcagaagc
    14941 tcctcaggag actcggcata gcagacgctg gtttttgtcc accgattgcc gtctttcacc
    15001 aatgaacaac caggcttcac caccacggga aaatcaacaa ttccctgtac gacccgttcg
    15061 acccgacctt caggaacaaa aattgtatcg ggaattgcta caccctccgt aatagcctgt
    15121 tgcatcaatc gatacttatc ggagatttct tgaaatgcct ggggactggg agccggaaaa
    15181 tgcgtatatt tttcgaattg agccttctct ggtccgagga catgcatggc aatatcagac
    15241 aaaggaatta aggcatcgac tcgatgggtt cgcactgcct ccatcaatgt ggcgatgaaa
    15301 cgttcaggtt cttgataagg agacggatag gcaatactct ctcgacagta acgcgaggaa
    15361 cctgcaaggg atcgttggga ctctgcccca acaatcacct ctacttgttc acctccaagt
    15421 gccctggtga ccgcaagcgc agcccgctca tttccgtcgg taatcaatac tttcacaagt
    15481 attcccgccc caaagcgatt cccctcgtat ccatcgccct tgttgactta catcgaactc
    15541 ggttggggac cgaacaatcg gaaagaaaaa agcgggatgg tgtccgatat gtcgttgtgg
    15601 attcctaccc ggcgaatggc atacctattt tctgggcagc tcccttcgaa gcccattc
//
LOCUS       DAORQN010000025        15314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_253_length_15314_cov_32.416213, whole genome shotgun sequence.
ACCESSION   DAORQN010000025 DAORQN010000000
VERSION     DAORQN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_253_length_15314_cov_32.416213"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK614_03080"
     CDS             <1..303
                     /locus_tag="PK614_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81221.1"
                     /translation="QAMRTGGVLTIRTSMDEAHCLVRIQDSGCGISSSVLPRIFDPFF
                     TTKNEGEGTGLGLSVSLGIVERHGGRIFVESEVGKGTTFTVSIPLATERHAIGRFS"
     gene            300..1751
                     /locus_tag="PK614_03085"
     CDS             300..1751
                     /locus_tag="PK614_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB81222.1"
                     /translation="MKGLKVLIVDDEPLMRLSMLDALEGIGCEVMAAATGTEGLTILG
                     TRQFDIVITDLRLPGADGLTILKACKERSPTTEVILITAHGSVDTAVGAIKLGAYDYI
                     TKPFQMDELLLIVERVGKILGLRRENLELKEVLEDRFSFGGILGCNHQMRALLEKIKL
                     VAATDSPVSIVGERGTGKELVAHAIHLNSPRRDQPLIKVCCADLPEALLEAELFGHEK
                     GAFPGALRQRRGRFELAHKGTLLIDDIDAVSPNVQARVWRVLQNRRFERVGGREHIEV
                     DVRIVCASKDDLKGMAYQGRFHKELSDQLTAVQIVVPPLRERREDVLVIAEHMLEARS
                     AALGKSLDGFAQPSRDLLLHYSFPGNVGELEQMVDRAAALGRDGESLQPWDLCGFQTC
                     PYLGGSPQAGCGFCSEGLTAMGEKPESTAPATLATAREAFERDYIAAVLKQVDGSRTN
                     AATILGVSRKALWDKCKRYGLSSAKGEAEEKDE"
     gene            complement(1753..2862)
                     /locus_tag="PK614_03090"
     CDS             complement(1753..2862)
                     /locus_tag="PK614_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRB81223.1"
                     /translation="MSNQTTAPLTAPSTGQPSSQSGTAQLPPPVDHLEYWKAGLRELK
                     TFRGHSHGVWSVAYAPDGQTIVSGGVDRYVRVWDIETGRLLRSLRGHTADIRALVFTP
                     DGRTLASGSEDRTIRLWNPKTGEPTKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIK
                     IWEVTTGTDLMTLLGKDQYDHHWSVCVAFSPDGVHLASGSDIGKIRIWEVLPSGEEKI
                     LHNGHWEETAEDSTETRGFFIEDDGGFQKPMEYWIGAMVFTPDAKMLITGSRDNTIRF
                     FEMPTMNELRVVRGHNGWVRSLVVSPDGKVLISAGDDNTIRFWDIATGRNFRTDKTHG
                     GAVRGIALSPDGLRLASASWDRTVKLWEGGVEPAE"
     gene            3061..4092
                     /locus_tag="PK614_03095"
     CDS             3061..4092
                     /locus_tag="PK614_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81224.1"
                     /translation="MATGVEVSKPSMEIRFQPALLQEVIDSFIEKTEREGDPTYYKEF
                     HELADPIYEKFTLDDREAEFKKLYQYLFGIWGFSDIIRDAFNEFPELKQRVGIVLVKG
                     VLKEDQEGVDVLRKWGSVEHDLAKQFEEKGLKGVGIKLIPRRFYDPALTRYCRHELLH
                     IHDMLDPAFGYDPDTRVGQNPGEETLILHRYRILWNISVDSRLIASGKEPMLQKEDRF
                     KEFRSWYRKIPPAQLKSVFEGLWQTSYFTHSELVEMATDTLRILDRAVDVEGGEIPET
                     TNKVMLMPGFPCPLCRFPTYTWVEDMETKLEKYILDFIRENHPGWDVEYGACDRCVEV
                     YKLRADGVM"
     gene            4105..4710
                     /locus_tag="PK614_03100"
     CDS             4105..4710
                     /locus_tag="PK614_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRB81225.1"
                     /translation="MAADPKYGRRDFLKDSVVSVAKAAREFAAHKDAPREQPAAPVRT
                     DWLRPPGAVNEAMFLERCTRCSDCIKVCPPGAIVSDVANGTPVIFSNQVACELCNDFP
                     CIAACATEALLPVAGCFDVRMGVATVSHRVCTAGQGCHACVSKCPVEALSMDFHALHL
                     VVAPERCVGCGMCEQICKTVNDRIAIKVTPARNLSAGALGY"
     gene            4921..5898
                     /locus_tag="PK614_03105"
     CDS             4921..5898
                     /locus_tag="PK614_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRB81226.1"
                     /translation="MTSKQVVQPTSPSSTAIAPPQAVAIKDSPAVERALNRSKIYLLF
                     SWSLLYPEDEEFLDYLQCGEFVEDGRAALDGLRLALDGIGGDRASQKIALMKKQFDQI
                     EKLVSAECVNWQIGDLQTEHRRVFTNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFY
                     KAFGVELSKDVHERLDHLSVELEFMHFLTYKESYSRCHDGIDKTEIVVDAQKKFIKNH
                     IGRWVPLFCRMLAKKSDTGLFKLIADCMSEWMDFEVAFLGVTVQPYSEADYRPATFNA
                     PEGQTYECGAQDKGNELSMLLSEVGAESFMDQQTKEKGGEKSEGPVGTA"
     gene            6007..6951
                     /locus_tag="PK614_03110"
     CDS             6007..6951
                     /locus_tag="PK614_03110"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRB81227.1"
                     /translation="MQTRNKRLVFGILFSALIVGIMLTIGQVPLAVSQPVTIPVKAIK
                     GVIPMDGANPIWEGVPGVIIPLSGQTITTPMHPNISVKSVFVKAVSNGKDLGLRLDWS
                     DQTKNDTAIGPQDFRDQAAVMFPVNTAGAPPFQCMGQSGGTVNIWRWNAEWQKDLGKD
                     SAGMWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNSGIWSGNIMSDPTLRVS
                     SVEDLNANGFSTLTTQAHQDVIGNGVWEPSGSLKGGGYTGPTWRVVVKRSLETSDAND
                     TQFKAGASVPIAFAIWDGSNIERNGMKALSTWFTLKLP"
     gene            7227..9008
                     /locus_tag="PK614_03115"
     CDS             7227..9008
                     /locus_tag="PK614_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB81228.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLTQAGVKNVSQRDLGIEL
                     LDKVLTQSFAHGLYQQLVDKQQGLERERTGERGPGSAEQLARVIESLDRFPYLFERIE
                     LAKETLRGEGFYDIEAYRNSLFLIDKWLEVLSSLYFPTRMTVVDNQFGDYSIYSSKDL
                     IKAIRDEGQNPYISLFREHVLPSLLTDRPDLVGVSITATSQIIPGLTLCRLIKEHAPE
                     LHVTVGGSIFTRLVDNLRRCPWLFDLVDDFVVFEGETALLELVNQMDGRRDFSKVPNL
                     IYRQNGKITVNQPFYSENINQLTAPNYDGFPLDLYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQRDPGKTVDDLVWLKERYGAQSFFFTDECFALSPTKRLCQQMIDRQVN
                     VKWTCELRFEKNLTRELLGQMRDAGCLKIVFGLESFNQRVMDFMKKGIKQESVRRIVD
                     DCVDLGIAVHCYVIVGFPTEKEEEALDTMNFVVGNKKLHESYGFSCQPCLFDLEKEAP
                     IMSDPGSYGIRRIMRPATEDLSLGFFYEVQEGMTPAQAEQLYQQVYEKISEVVCELPF
                     NYSMADGLLYIARAKSQALQVPQPTGS"
     gene            9098..10219
                     /locus_tag="PK614_03120"
     CDS             9098..10219
                     /locus_tag="PK614_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81229.1"
                     /translation="MSAVATAVERSTGRVEDQGLLFEYTIKLVLFVGFFELVLYRLVS
                     RLGMHISKIAAQHEWVGTIFQLLTSVGFALLNVVAIFVFLALVVLLVNRARAKGITGF
                     NVVTIPSVALLLILTVSFLIVPPAMLGSIAYNVVTLIALTALMLEYLSQQQEWSKRVM
                     GAVYYFGISGWLYYQIFSTAYGWMGVIAAPPFVYEANRLGEALMVLASILVFWAYGRG
                     VSFRTRNRQQRRRAIWFSAVSAVVFFGLLFMDYFLGLYNPVLAHSIRKAGEGISWIFQ
                     MGMGYTFYLPFAFYVAGLLCWSYTVIRLVTMGRLAGYGLALMFIAGYALLFSSLTLMV
                     VLGVMLMTLDRPKGAVTEQAAVTRPPLVGTQESLVGGQI"
     gene            10253..11065
                     /locus_tag="PK614_03125"
     CDS             10253..11065
                     /locus_tag="PK614_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81230.1"
                     /translation="MDEQTQASGAEQGSAAVDPGDQPLSPDEEIAEIEKLLGAEPDDF
                     QARCRLGELYFSKGRLDDALTEVKKSIEMAEGLRTEMNRSLAMYYSNLGTIYATKGMI
                     DEAEAEFKRALDVHAYDVLALFNLGRVQADKRKYMESKNYYERLVEITPDDPMAWYNL
                     AGVYVELDNPQVSDYNTMDMAIQCYLRVLELDPKHLEASFKLMEIALNHRKTDLAIKV
                     MESAVEHSPDEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKKAKEGAAS"
     gene            11090..11383
                     /gene="tatA"
                     /locus_tag="PK614_03130"
     CDS             11090..11383
                     /gene="tatA"
                     /locus_tag="PK614_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB81231.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAQAQAQAQQAEPVQAQAIQAPPPVAMSTPVVEQPVAAAPPAARA"
     gene            11430..12683
                     /locus_tag="PK614_03135"
     CDS             11430..12683
                     /locus_tag="PK614_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81232.1"
                     /translation="METDVQLAVGYIETFAGNGKARSTGDGKRAVKAGIPLPHHVALD
                     RDERWLYFAESGSDRVRRVNLAEGTVHNFAGIGETCYSGDEGPCGEAGLYLPLDVACD
                     SRNDLYVCDSGSNRIRKIDRETGIITTVVGTGEHGFNGDGPALEVNLTWPAAIAFDAD
                     DVMYIADTQAHRIRRYDSRTGLVETIAGSWTAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDNGWLMPVCSDGLSLSMYLDDGHPAMEARLYDIVGIAVDNAGDVYVVDKGSNRVRK
                     IDHKTGLISTLAGVCRYGYDGDGKPAAKSMLHAPEAIVFDQHNHAYISDTGNHRVRKV
                     DADTGFISTVAGNGDSGYDDKNMGGCGAARFVAKDAAGALKHGDGLLAVEAVVNSPVG
                     LTLDTQGYLYICERGENKIRRAKLW"
     gene            12768..13601
                     /locus_tag="PK614_03140"
     CDS             12768..13601
                     /locus_tag="PK614_03140"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRB81233.1"
                     /translation="MTSVHTSRTRLLTLLSSLFVLALILGGLGIPIVSSEGMIIRAQA
                     LSGDIPTAPEDPLWQKISPMTLPLSGQVITRPVWPEPTAHALAVRAVHNGTDIAFLLE
                     WQDNTKNDRLTPGTFRDGVALGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRA
                     SRTTEKDKAASGEPRRFEVIPRRASSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGTW
                     RVVMRRPLSSEEQENEAKLIPGRIQAVSFAVWNGENKERNGQKAIAPWFQLALDPATK
                     T"
     gene            13685..14158
                     /locus_tag="PK614_03145"
     CDS             13685..14158
                     /locus_tag="PK614_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRB81234.1"
                     /translation="MSKKNRTIFTRALSLGRALCVCGALFTVLGWGFQADAGTGMNGE
                     MTAEEAVQLGEEFGIAVGEVDEEVQKELKLQRPEGVAVFEVIGNSRADYAGIKVRSVI
                     KEIDKKEVRNLIDFGKAVKAAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            14183..>15314
                     /locus_tag="PK614_03150"
     CDS             14183..>15314
                     /locus_tag="PK614_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRB81235.1"
                     /translation="MNDKGRSIQKLAMGGVLLFMALLNITFHERAIAQKTDGQVPADW
                     VAEIEKIFIRSEDCKQCHDRHYEEWKGMREQTPDLKSFGRVDAALLHGTSFESPVFRT
                     VLGVWMQTNPTMQERQRCLSCHAPAVTVFPQHVEKISAQILSGKPQVEGIGCASCHLI
                     NAVETTPLPPPTYKVQPGEMLYGPYADPEENLVHPATQLPLFRGANFCTSCHFDKVKD
                     VTQKDLPGEILQGTICQDCHMEPSTGSSTSKRGAMTRAIGRHWFRGVVIPGTLLKNRN
                     LQAEWMPRIEIDATKSGAGVEGTVLVKVGSLPHSFPDGDPVLKQFFLAITIKDAQGNV
                     LGEDTKRFGLPYDKILRGPIPDPFIKGGNTRRVPFSQTLKDGA"
BASE COUNT         3186 a         3964 c         4792 g         3372 t
ORIGIN      
        1 caagcgatgc ggaccggtgg agtcctgacc atccgtacgt cgatggacga ggcccactgc
       61 ctggttcgga ttcaggattc aggctgcggc atttcctcct cggtgttgcc gcggattttc
      121 gatcccttct tcaccacgaa gaatgaaggg gagggcaccg ggcttggcct gtccgtcagc
      181 ctgggtattg tggagcggca tggagggagg atttttgtcg agagcgaagt cggcaaaggg
      241 accaccttta ccgtctctat tccgcttgcg acggaacggc acgccatcgg gaggttttcg
      301 tgaagggcct caaagtacta atcgttgatg atgagccgtt gatgcggttg tccatgctgg
      361 atgctttgga gggcatcggc tgtgaggtca tggcggccgc cactggaaca gaagggctga
      421 cgatcctggg cacgcgtcag ttcgatatcg tgattaccga cctgcgcctt cccggcgccg
      481 acggactgac gattttgaag gcctgcaagg agcggagccc gacgacggaa gtgatcctga
      541 ttacggccca tgggtcggtg gatacggcgg tcggtgcgat caagctgggt gcatacgact
      601 acatcaccaa gccgtttcaa atggacgagc tgctgctgat tgtcgagcgt gtcgggaaaa
      661 ttcttggatt gcgacgggaa aatcttgaat tgaaagaggt gctggaagac cggttcagtt
      721 tcggcgggat cctcgggtgc aatcatcaga tgcgggccct gctggagaag atcaagttag
      781 tggccgcaac cgattcgccg gtgtcgattg tcggggagcg cgggacgggc aaggagttgg
      841 tagcccatgc cattcatttg aatagtcccc ggcgcgatca gcccctgatc aaggtctgtt
      901 gcgcggacct tcccgaggcg ctcctggagg cagagttatt cggtcatgaa aagggcgcgt
      961 ttcccggggc gctgcgccaa cgacgtggac ggttcgaatt ggcgcacaag ggaaccctgc
     1021 ttattgacga tatcgatgcg gtctctccaa atgtgcaggc aagggtgtgg cgcgtgcttc
     1081 agaatcggcg attcgagcgt gtcggcgggc gcgaacacat cgaggtggac gtccggatcg
     1141 tgtgtgcgtc caaggatgat ctgaagggga tggcttacca gggacggttc cacaaagaac
     1201 tctccgatca gctcacggcc gtgcagatcg ttgtgccacc attgcgcgag cggcgggaag
     1261 atgtgctggt gatcgccgag catatgctgg aggcgaggtc ggcggctctg ggcaaatctc
     1321 tcgacgggtt tgcgcagccg agtcgcgatc tgctcctgca ctattcgttt cccggcaatg
     1381 tcggtgaact ggagcagatg gtggatcgag ccgccgcctt ggggcgggac ggggaatcgt
     1441 tacagccgtg ggacctatgt ggcttccaga cctgtcccta tctcggcggg tcacctcaag
     1501 caggttgcgg tttttgttcg gaaggactga cggcgatggg tgagaagccg gagtccacgg
     1561 ctcctgccac gctcgcgacg gcacgggagg cattcgagcg ggactatatt gccgcagttt
     1621 tgaaacaggt agacggcagc cggacgaatg ccgccacgat cctgggagta tcccgaaaag
     1681 ccttgtggga caaatgtaag cggtacggtc tctcctccgc caagggtgag gcggaggaga
     1741 aggatgagta ggttactcgg cgggctccac gccaccttcc cagagcttca ccgtacgatc
     1801 ccaggaggcg ctggccaggc gtagtccgtc cggcgagagg gcgataccgc gtaccgctcc
     1861 gccatgggtc ttgtcggtcc ggaaatttcg gcccgtggcg atgtcccaga agcggatcgt
     1921 gttgtcatcc cctgcactga tgagcacttt tccgtctggc gagaccacta acgaccgaac
     1981 ccatccatta tgcccgcgca cgacgcgcag ctcgttcatc gtcggcatct cgaaaaagcg
     2041 gattgtgttg tcacggctgc cggtgatcag cattttggca tccggcgtaa agaccatcgc
     2101 gccgatccag tactccatcg gcttctgaaa tcctccgtcg tcttcgatga aaaagccgcg
     2161 ggtctcggtc gagtcttcag cagtttcttc ccaatggccg ttatggagga ttttttcctc
     2221 tccgctcggc agcacttccc agattctgat tttgccgatg tcggacccgc tcgcgaggtg
     2281 cacgccgtcc ggcgagaacg ccacacagac cgaccagtgg tgatcgtatt ggtcttttcc
     2341 cagcagggtc atgaggtcgg tgccggtcgt gacttcccag atcttgatgt ctttattgtg
     2401 actgccgcgg gccagcatga ggccgtcagg agacaacgac aggctgcaga cattgtgatc
     2461 gtagcgggta aataagagtt tggtcggctc gccggttttc gggttccaca ggcggatggt
     2521 tcgatcttcg ctgcctgagg ccagcgtgcg gccgtccggc gtgaacacca gggcgcgaat
     2581 gtcggcggtg tggcccctca gcgaacgcag gagtcgcccg gtttcaatgt cccagacgcg
     2641 gacgtatcga tcgacgccgc cactgacaat cgtctggcca tcgggagcgt aggcgacgga
     2701 ccagacgccg tgggagtgtc cccgaaacgt cttgagttct cgaagccctg ctttccaata
     2761 ttctaaatgg tcgacgggag gggggagctg tgcggttccg ctctggctgc ttggttgtcc
     2821 ggttgaaggc gcggtcaggg gggcggtcgt ttgattgctc ataaaagtct gtgcctcgtc
     2881 gctgtgctga gcgggggcca tcgagccccg gccgggaccc tcaacggttt gcaaaaatcg
     2941 ctaaggggtt tgtataatcg gcggtcgttt cgatgatcgt aagagaacga ctgggagcaa
     3001 gtcaagcaca gtacccgtgc ggctttcatc ccttcaccga accagaacat gtgaggtctt
     3061 atggcaaccg gagtagaagt ttccaagccg tcgatggaga tccgttttca gccggcctta
     3121 ctgcaggaag tcatcgactc ctttattgaa aagacggagc gggaagggga tccgacctac
     3181 tataaagagt ttcatgaact ggccgacccg atctatgaaa agttcacgct ggacgatcgc
     3241 gaggccgagt tcaagaagct gtatcagtac ttattcggta tctgggggtt ctccgatatc
     3301 atccgcgacg cgttcaatga atttcctgag ctgaagcagc gagtcggaat cgtcctggtc
     3361 aagggggtgc tcaaggagga tcaggaaggg gtggacgtcc tgcgcaaatg gggctccgtc
     3421 gagcacgacc tggcgaagca attcgaggag aagggactga agggagtcgg tatcaagctg
     3481 attccgcggc ggttctacga tccggcgttg acgcggtact gtcgtcacga actcctgcac
     3541 atccatgaca tgctcgaccc ggcgttcggg tacgatcccg atacgcgggt cggacagaat
     3601 cccggcgaag aaacgctcat cctgcaccgc tatcgtattc tttggaatat cagcgtcgat
     3661 agccgcttga tcgcctccgg gaaggagccg atgctccaga aggaagaccg attcaaggag
     3721 ttccggtcgt ggtatcgaaa gattccgccg gcgcagttga aatccgtatt tgaggggttg
     3781 tggcagacga gctattttac tcattccgaa ttggtggaaa tggccactga taccctgcgc
     3841 atcctggatc gcgcggtgga tgtcgagggc ggagaaattc cggagaccac caataaggtg
     3901 atgctcatgc cggggtttcc ttgtccgctc tgccggttcc cgacgtacac ctgggtggaa
     3961 gatatggaga ccaagctgga aaaatatatc ttggacttca tccgcgagaa ccatccaggc
     4021 tgggatgtcg agtacggggc ctgtgatcgt tgtgtagaag tgtacaagtt gcgggccgac
     4081 ggtgtgatgt aagcggggtg catcgtggcc gctgatccta aatacgggcg tcgtgatttc
     4141 ctgaaagact ccgttgtgtc ggtggcgaag gcggcgcggg aatttgccgc tcataaggac
     4201 gcgccccgcg agcaaccggc cgcgccggtg cgaacagact ggctgcgtcc gccgggtgct
     4261 gtcaatgagg ccatgtttct tgaacggtgc acgcgctgta gcgattgcat taaagtatgc
     4321 ccgccgggag cgatcgtgag cgatgtggcg aacggtacgc cggtcatttt ttcgaatcag
     4381 gtcgcatgcg aattgtgcaa cgattttccc tgtattgctg cctgtgcgac cgaggcgctc
     4441 ctgccggttg ccggttgttt tgacgtgcgg atgggtgtgg caacggtgtc gcaccgtgtg
     4501 tgtacggcgg gccagggttg tcatgcctgt gtgtcaaaat gccctgttga ggccctctcg
     4561 atggattttc acgcgcttca tctggtggtg gctccagagc ggtgcgtcgg atgtggtatg
     4621 tgtgagcaga tttgcaagac ggtcaatgat cgtattgcga tcaaggtgac accggcaaga
     4681 aacctgtctg ccggtgcgct gggctactga ataaagtgtg tgtttactcg ttccggaatg
     4741 cgaaatgatc aggcagagta gtgaaacgtc ggcacttgcg gcaagggcaa aggagagaga
     4801 gttcatgctt gacatccctc cctcctttac ctatcatacg cctcctgtgc cgcaggactc
     4861 agatgttgcg tagtgtgcga aaaaaatgcg caacatgttg aggtgaggag caagatacgt
     4921 atgacatcga aacaggtcgt gcagcccacc tccccgtcct ccaccgcaat cgctcccccg
     4981 caggcggtcg ccataaaaga ttcgcccgct gtcgaacgcg cgctcaatcg cagtaaaata
     5041 tatctcttgt tttcatggag tttgttgtat cctgaggacg aggagttcct ggattatctc
     5101 cagtgtggtg aattcgttga agatggacga gccgcattgg acggattgcg tctcgcgctc
     5161 gatggcatcg gcggtgatcg ggccagccag aaaatcgccc tgatgaaaaa acagttcgac
     5221 cagatcgaga aactggtttc ggctgagtgt gtcaattggc agatcggcga tcttcagaca
     5281 gagcatcggc gggtcttcac caatgtgatc acgctggatt gtccgcccta cgaaacactg
     5341 ttcggtaacg atcacgtttt tgcgcagtcc catgtgatgg gcgacattgc aggcttctac
     5401 aaagccttcg gcgtcgagct ctcaaaggac gtgcacgagc gtctcgatca cctgagcgtc
     5461 gagctcgagt ttatgcattt tctgacctac aaagaatcct attcgcgctg ccacgatggt
     5521 atcgacaaga ccgagatcgt ggtcgatgcg caaaagaaat ttatcaagaa tcatatcggc
     5581 cgatgggtgc cgttgttctg cagaatgttg gcgaagaagt cggatacggg cttattcaag
     5641 cttattgccg actgtatgtc ggagtggatg gattttgagg tcgccttcct cggagtgacc
     5701 gtgcaaccgt actctgaggc ggactataga ccagctacct tcaatgctcc ggaaggtcaa
     5761 acctacgagt gcggggcgca ggacaaaggg aatgagctca gcatgttgct gagtgaggtc
     5821 ggcgctgagt ccttcatgga ccaacagacg aaagagaagg gcggcgagaa gagcgaaggc
     5881 ccagtcggga ctgcgtagga gttctcggtt ttcgggcagt gcagtattcg gttttcggca
     5941 gacgttatac gttcccagtt ctttttctta ttaatctttc agaggagggc atacgcaatg
     6001 agactggtgc agacacgcaa taagcgtttg gtgtttggca ttctgttctc tgcgctgatc
     6061 gtcggcatta tgttgacgat tgggcaggtg cctctcgcgg tcagccaacc ggtgaccatt
     6121 cccgtgaagg cgatcaaggg ggtaatcccg atggatggtg caaaccccat ctgggaagga
     6181 gtgccaggtg tcatcatccc attgagcggc cagaccatca cgaccccgat gcacccgaat
     6241 atttcggtga agtcggtgtt tgtgaaggcg gtcagcaatg ggaaggattt ggggttgcgc
     6301 cttgattgga gcgaccagac caagaacgac acggcaatcg gtccgcagga tttccgggac
     6361 caggccgccg tcatgttccc ggtcaacacc gcgggagccc cgccattcca gtgcatgggc
     6421 cagtcaggtg ggacggtcaa catctggcgt tggaacgcgg agtggcagaa ggatctcggc
     6481 aaggatagcg ctgggatgtg ggacgtggat gaccaatacc ctggcatctt ctgggattac
     6541 tatttcgaag agccggctgg tggagtgacc tatccggatc gtattggccg gagccttggc
     6601 ccattcaatt ccggcatttg gtccggtaac atcatgtccg acccgaccct gcgcgtcagc
     6661 tccgtcgagg atctgaatgc caacgggttc agcaccttga ccacccaggc ccatcaggat
     6721 gtgatcggca atggtgtgtg ggagccgtcc gggtccctca agggcggtgg atacaccggc
     6781 ccgacctggc gtgtagtcgt gaagcgttcg ctggagacca gcgacgccaa cgacacccag
     6841 ttcaaggcgg gcgcttcggt gccaatcgcc ttcgcaatct gggatggttc caacattgaa
     6901 cgaaacggta tgaaagcgct ctccacttgg ttcacgctga agttgccgtg agcctagtgg
     6961 cctgaagtgg ccaatcctac tcctgtgccg ggtaggagtc gtgtggtgat tcgtaacagg
     7021 aaggccccga gtcgttgcca gaaatggcaa agtgattcgg ggccttcgtg cgttttgacg
     7081 gagagggctc aacttcgggg aggcctgaaa tttcttggcg tgcacagggc ggagggttgc
     7141 attcggactg gagcggagtg tatataccga tacggtgcaa ctcttctgaa aatactgatt
     7201 tttctgctta atataaagat aatcctatga aagtttcact cctgtttcct cctacgtggc
     7261 acccatccca accgtacctc agccttccat cactcaccgg attcctcacc caagccggag
     7321 tcaaaaacgt ctctcaacgt gacctcggca ttgagttgtt ggacaaggtt ttgacgcaat
     7381 cctttgcgca tgggctctac cagcagttgg tggacaaaca gcaaggtctg gagcgagagc
     7441 gaaccggtga gagggggccc gggagcgcag agcaacttgc tcgcgtgatt gagtcgcttg
     7501 accggtttcc ctatttgttt gagcgaatcg aactggcaaa agagacgctg cgaggtgaag
     7561 ggttctacga tattgaggcc tatcgcaata gtctctttct gatcgataaa tggcttgagg
     7621 ttctctcctc gctgtacttt ccgacgcgaa tgacagtggt cgataaccaa ttcggcgact
     7681 attcgatcta ctcctcaaaa gatctgatca aggccattcg tgacgaaggg cagaatccct
     7741 atattagtct cttccgcgag cacgtgttgc cttcgctcct cactgatcgt ccggatctgg
     7801 tcggtgtctc gattacagcg acctcgcaaa ttattccagg cctcacgctc tgtcgcctga
     7861 tcaaggagca tgctccggag ctacacgtga cggtcggagg aagtatcttc acccggttgg
     7921 ttgataatct gcgccgctgt ccgtggctct tcgatctcgt cgatgatttc gtcgtgtttg
     7981 aaggtgaaac agccttgctg gagttggtga atcagatgga tggcaggcgg gatttcagta
     8041 aggtcccgaa tctgatttat cgtcagaacg gtaagattac ggtcaatcag cccttttact
     8101 ctgagaatat caaccagctc acagcgccga actacgatgg ctttccgctg gatctgtatt
     8161 tgtcccccga gcctgtcctg ccggtacaat tttcacgagg ctgctactac aaagattgcg
     8221 cgttctgcgc cctcacgctg gatcaccaga atttccggca gcgtgatccg gggaagacgg
     8281 tggacgattt ggtctggttg aaagagcgct atggagcgca gtcctttttc ttcaccgacg
     8341 aatgtttcgc cctctctccc accaagcgtt tgtgccagca gatgatcgat cggcaagtga
     8401 atgtgaagtg gacctgcgag ttgcgtttcg agaagaacct cacgcgtgaa ttgcttggcc
     8461 agatgaggga cgccggctgt ttgaaaattg tgttcggcct ggagtcgttc aatcagcgag
     8521 tcatggactt catgaaaaaa gggatcaagc aggagtccgt gcgccggatc gtcgatgact
     8581 gcgtcgatct tgggattgcc gtgcactgct acgtgatcgt cgggttcccc acggaaaagg
     8641 aagaggaagc cctcgatacc atgaacttcg tggtggggaa taagaagctc cacgagtcct
     8701 acgggttctc ctgtcagccc tgcttgttcg acctggaaaa agaggcgccg atcatgagtg
     8761 atccgggcag ttacggcatc cgtcggatca tgcggccggc gaccgaagac ctgagcctgg
     8821 gcttctttta cgaagtgcag gagggcatga cccctgctca ggccgagcaa ctctatcaac
     8881 aggtttacga aaaaatcagc gaagtcgtgt gcgaattgcc gtttaactat tctatggccg
     8941 acgggctgct ctatatcgct cgggccaagt cgcaagcgtt gcaggtacca cagccgaccg
     9001 gctcttagcg ttcgtgcggt catcagggca ggctcctgcc gcgttgaccg gttgtggtca
     9061 gaggattatc atcaccgttt tgcacagggg gctggagatg tctgctgtag ccaccgccgt
     9121 tgaacggtca accgggcggg tcgaagatca gggactgttg ttcgaataca ccatcaagct
     9181 ggtgttgttc gtcgggtttt tcgaactcgt cctctatcgc ctggtatccc gactcggcat
     9241 gcatatcagc aagattgcgg cccagcatga gtgggtcgga acgatcttcc agttgctgac
     9301 gtctgtcggc ttcgccttgc tgaatgtcgt cgcgatcttt gtatttctgg ccctggtggt
     9361 tctgctggtg aacagggctc gcgccaaggg catcacgggg tttaatgtcg tcactattcc
     9421 cagcgtggcc ctcctgttga tcctcacggt gtcctttctg attgtcccgc cggccatgct
     9481 gggctccatc gcctacaatg tcgtgaccct cattgcactc accgcgctga tgctggagta
     9541 tctctcccag cagcaggagt ggtcgaagcg agtgatgggc gcggtctatt acttcggcat
     9601 ttccggatgg ctctattatc aaattttctc caccgcctat gggtggatgg gggtgattgc
     9661 cgcaccgcca tttgtgtatg aagccaatcg tctcggcgaa gcgctgatgg tgcttgccag
     9721 catcctggtg ttctgggcct atgggcgcgg ggtgtcgttc cgtaccagga atcgacagca
     9781 gcgccggcgc gcgatctggt tcagcgccgt ctcggcggtg gtgttctttg gcttactgtt
     9841 tatggattat ttcctggggc tctacaaccc ggtgttggcc cattccatcc ggaaagccgg
     9901 ggaggggatc agctggatct ttcagatggg catggggtat accttttacc tgccctttgc
     9961 cttttatgtc gcggggctgc tctgttggtc gtacaccgtg atcaggctcg tgacgatggg
    10021 gcgtcttgcg ggatacggcc tggcgttgat gtttattgcg ggctatgcat tgctcttttc
    10081 cagtttgacc ctgatggtgg tgctcggggt gatgctgatg acgcttgatc gtcccaaagg
    10141 tgcggtgacg gaacaggcgg cggtgaccag gccgcccctc gtcggcacgc aggaatctct
    10201 cgttggtgga caaatttaat cttatgagtc acccagatta ggatacgttg ttatggatga
    10261 acagacacag gcaagcggag cagagcaggg gagtgcggcg gtggatcccg gcgatcaacc
    10321 gttgagcccc gacgaagaaa ttgcggagat tgaaaagttg ctcggtgccg agcccgatga
    10381 ttttcaggca cggtgccggt tgggggaact gtatttcagc aaaggccggc tcgacgacgc
    10441 gctcaccgaa gtgaaaaagt cgatcgagat ggcggagggg ctccggaccg aaatgaaccg
    10501 gtcgctcgcg atgtactact ccaacctcgg gaccatctat gccaccaagg gtatgatcga
    10561 cgaggctgaa gcggaattca agcgcgcgct cgatgtgcat gcctacgatg tgcttgccct
    10621 gttcaatctg ggccgcgtgc aggcggacaa acgaaagtac atggagtcga agaattacta
    10681 cgagcggctc gtcgagatca cgccggacga tccgatggca tggtacaatc tcgccggtgt
    10741 gtacgtggaa ctcgacaacc cacaagtctc cgattacaac acgatggaca tggcaatcca
    10801 gtgttatctt cgtgttctgg agttggatcc gaagcatctg gaggcgagct tcaaactgat
    10861 ggaaatcgcc ctcaatcatc ggaagacgga tttggcgatc aaggtcatgg agagcgcggt
    10921 ggagcacagc ccggacgagc ccctcgccta ctacaacttg atcagcgtct acgacaagtg
    10981 caagatgttc gagcaggccg aacaggctcg ccagcgcttg aaagaacgat tcgcgaagaa
    11041 agccaaagag ggagccgcat cctgaacatg tgagcaaagg agacacacca tgtttggcag
    11101 tcttggtttt acagagttga tcctgatcct gtttattgtg ctgattattt tcggggcagg
    11161 gaagttgccg cagcttggcg aggggatcgg taaagcgatc aagggcttca agaaatccgt
    11221 tcatgaggcg gatgcgatcg aggctgaagc ccaggcgcaa gcgcaggctc aacaggccga
    11281 accggtccag gctcaggcta ttcaggcccc gccgccggtg gcaatgtcca cgcctgtcgt
    11341 tgagcagccg gtcgccgcag cgcccccggc cgcgcgcgcg tagcatcctt cgacacaatg
    11401 ggcccagcac agaacggatt gacacagtta tggaaacgga cgttcaatta gcggtcggat
    11461 acattgaaac ctttgccgga aacgggaagg cgcgaagcac gggggacggc aagcgcgctg
    11521 tgaaagcagg cattccgctg ccgcaccacg tcgcgctgga tcgggacgag cgctggctgt
    11581 atttcgccga atcaggcagc gaccgggttc gtcgcgtcaa tcttgcggag ggtaccgtcc
    11641 ataattttgc cgggatcggg gagacctgct acagcggcga cgaagggcct tgcggagaag
    11701 ccggactgta tctaccgctg gacgtcgcct gtgactctcg taacgatctc tatgtctgtg
    11761 attccggcag taaccggatt cgaaaaatcg atcgtgaaac cggaattatc accacggtcg
    11821 tgggcaccgg ggaacatggg tttaatggtg atggcccggc gttggaagtg aatttgacct
    11881 ggcccgcggc gatcgccttc gacgcggacg atgtgatgta tatcgccgac acgcaggcgc
    11941 accggattcg tcggtacgac tcgcgaaccg gcctggtgga aaccatcgcg ggaagctgga
    12001 ccgcggaaga cgaagcccgg gagcagccat tggtggcgcg caacctggtg gtgttgtccg
    12061 gcgatgcgat cggcatcgac ttcagtgacg acaacgggtg gctgatgccg gtctgttcgg
    12121 atgggctcag tttgtcgatg tacctcgatg acggacatcc ggccatggag gcgcggctgt
    12181 acgatatcgt cggtatcgcc gtcgacaatg ccggtgatgt gtatgttgtc gacaagggca
    12241 gtaatcgagt gcgcaagatc gatcataaga ccgggctgat ttccaccctc gccggtgtat
    12301 gccggtatgg atacgatgga gacgggaagc cggcggcaaa gtcgatgttg cacgcgccgg
    12361 aagccattgt cttcgatcaa cacaatcatg cctatatctc tgacaccggc aaccatcggg
    12421 tccggaaggt ggatgcggac acgggcttca tcagcacggt ggccggtaac ggtgacagcg
    12481 ggtacgatga taagaacatg ggtggctgtg gcgcggcgcg gtttgtcgcc aaagatgccg
    12541 ccggcgcgct taagcatggt gatggtctgc tcgccgtcga ggccgtggtc aattctccgg
    12601 tggggctgac gctcgacacg caggggtact tgtatatctg tgagcggggt gaaaataaga
    12661 ttcgtcgcgc caagctgtgg taatttgggg aacggattca gcgtcccgcg cacacagcgg
    12721 gtgcgggagt tttcgcggtt agcggattgc gtcgggacat gaggcgaatg acatcggttc
    12781 acacatcccg cactcggtta ctgacacttc tctccagcct gttcgttctc gccctcattc
    12841 tgggcggtct cggtatcccc atcgtcagtt cagaaggcat gatcatccgt gcacaggccc
    12901 tctcgggaga catcccgacc gcgcccgaag atcccctctg gcaaaaaatt tcgcccatga
    12961 ccttgccatt gagcggccag gtgatcaccc ggccggtgtg gccggagccg acggcccatg
    13021 cgttggcagt ccgcgccgtg cacaacggaa ccgacatcgc cttcttattg gaatggcagg
    13081 acaatacgaa aaacgatcgc ttgacgcctg gcaccttccg tgacggtgtt gcgctgggat
    13141 tgccgctcgg cgatgcgccg gcatttttct gtatgggtca gctcgatcat tacatcaata
    13201 tttggcattg gaaagccgat tggcagagcg acatcgaccg gcgtgcttcc aggaccacgg
    13261 aaaaagacaa ggcggccagt ggtgaacccc ggcgctttga ggtcattccg cggcgggcgt
    13321 cctcggtgga agatttgatc ggcggtgggt tcagcaccct gaccaccaag gagaagcagg
    13381 ggcgcgtgca gggaaaagcg acgtggaaag acggcacgtg gcgagtcgtg atgcgtcggc
    13441 cgctatcgag tgaagagcag gagaatgaag cgaagctgat tccgggtcgt attcaggccg
    13501 tgtcttttgc cgtgtggaac ggcgagaaca aagagcgtaa cggacagaaa gccattgccc
    13561 cctggtttca actcgcgctg gatccggcca caaagacgta attcttcctg gtaggatatg
    13621 actgtcggca ggacgtgccg gttccggttc aacctgtgcg gatgaggcga acccgttgga
    13681 gggaatgtcc aagaagaatc gaacgatttt caccagagcc ctttctctcg gaagggctct
    13741 ttgtgtgtgt ggggccttgt tcaccgtgtt gggatggggc ttccaggccg atgcgggtac
    13801 cggtatgaac ggcgagatga cggcggagga agccgtgcaa ctcggggaag agttcgggat
    13861 cgcggtcggt gaagtggatg aagaggtaca gaaagaactc aagttacagc ggcctgaagg
    13921 cgtggcggtc tttgaagtga tcggcaattc cagagcggac tacgcaggga tcaaggtgcg
    13981 gtcggtgatc aaggagatcg acaagaagga agtccgcaac ctgatcgatt tcgggaaggc
    14041 cgtcaaggcg gcgatgaaag agtgcaattt taccgtcggg acctatgagc cggcagatcc
    14101 gggcgatccg gtcggctggg gcgtgaattt tcattttgtc gggtgcaagc gggactaaat
    14161 ccgaaccggt catgagcgca gcgtgaacga caagggacga tcgattcaaa agttggcgat
    14221 ggggggggtg ctgctgttca tggcattgct caacatcacc tttcatgaac gggcgatcgc
    14281 gcagaagacc gatggccagg taccggctga ttgggtggcg gagatcgaaa agattttcat
    14341 ccggtcggaa gattgcaagc agtgccacga tcgtcattac gaagaatgga agggcatgcg
    14401 cgagcaaact ccggacctga aatcgttcgg gcgggtcgat gccgccttgt tgcatggtac
    14461 ctcgttcgaa tcgccggtgt tccggacggt gctgggggta tggatgcaga ccaatccgac
    14521 gatgcaggag cgacaacgtt gtttgtcgtg tcatgccccg gccgtgacgg tcttcccgca
    14581 gcatgtcgag aagatcagcg cgcaaatcct gtcaggcaag ccgcaggtcg agggaatcgg
    14641 ctgtgcgtcc tgtcatctca tcaacgccgt cgaaacgacg ccgctgcccc ctccaaccta
    14701 caaggtgcag ccgggcgaga tgctctacgg tccctatgcc gatccggaag agaacttggt
    14761 acatccggcg acacagttgc cgctgtttcg tggcgcgaat ttctgcacgt cctgccattt
    14821 cgacaaggtg aaggatgtca cgcagaagga tttacccggc gagattctgc aagggaccat
    14881 ctgccaggat tgccatatgg agccctccac gggaagctcc acgtcgaaac gcggcgccat
    14941 gacccgcgcg atcggccgcc attggtttcg aggcgttgtc attccaggga cgttgctgaa
    15001 gaaccgcaac ctgcaggcgg aatggatgcc gcgtatcgag atagacgcca cgaaatccgg
    15061 agcgggagtg gaaggcactg tgctggtgaa agtcggcagt ctgccgcata gtttccccga
    15121 cggcgatccg gtgttgaagc aattcttcct tgcgatcacg atcaaggatg cgcaagggaa
    15181 tgtgttgggt gaagacacga aacgattcgg tcttccgtac gacaagattc tacgcggccc
    15241 gattccggac ccgttcatca agggcggcaa tacgcgccgt gtcccgttct ctcaaacgct
    15301 gaaggatggc gccg
//
LOCUS       DAORQN010000026        15111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_257_length_15111_cov_19.311769, whole genome shotgun sequence.
ACCESSION   DAORQN010000026 DAORQN010000000
VERSION     DAORQN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_15111_cov_19.311769"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK614_03155"
     CDS             <1..603
                     /locus_tag="PK614_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB81236.1"
                     /translation="AREVAAAIQAKGGVAAVIQADMSRVAEAQRLVQETVHQFHRLDI
                     LVNNAGRFIPKKLVDTTETEFDAIIALNAKGPYFAMQAAAGVLSDGGRIVNISSALTH
                     LKFPGATAHLGSKAALEQYGKGLAQELAPRGITVNTVLPGFTDTGVLTEQYREMGEQM
                     SPFKRLGLPSDVADVVAFLVSEQARWLTGQVIQAGGGLVM"
     gene            621..1004
                     /locus_tag="PK614_03160"
     CDS             621..1004
                     /locus_tag="PK614_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRB81237.1"
                     /translation="MARQNVSTGGPWEAKIGYSRAVRVGQSVQVSGTTAMTATGLVGK
                     GDPYAQTIQALKTIEAALVQAGASIADVVRTRIYMANIDQWQEVGRAHGEVFGSIRPA
                     TTMVEVKRLIDPDMLIEIEADAIVS"
     gene            1188..1865
                     /locus_tag="PK614_03165"
     CDS             1188..1865
                     /locus_tag="PK614_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRB81238.1"
                     /translation="MNQQSPEIAHLCKIDPVMKRVIGEVGPYALSLRARRSPFESLAR
                     AIAYQQLHDKAAESILRRFIALFPGRRFPRPADLLAVTPELIRGTGFSRAKIAALQDL
                     AAKAIEGIVPTTTVIQRLEDDAIVERLIAVRGVGRWTVEMLLIFQLGRPDVLPVDDFG
                     VRNGFRIAYKRKAMPTPKELLVYGERWRPYRTAAAWYLWRAADRAKVVRTTLPVVRAA
                     GKRAPNG"
     gene            1858..2721
                     /locus_tag="PK614_03170"
     CDS             1858..2721
                     /locus_tag="PK614_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB81239.1"
                     /translation="MADLRKRLDANRAGNFYVDSTCINCDACRQLAPSSFEEVGEYSA
                     VTHQPATEPERRQAYRALLACPTGSIGAEHTNQTLLSAAKADFPLPIEGNVYYCGFNS
                     EKSFGANSFFVQHPDGNWLIDSPRYLKPLVDVFDRMGGLAQIFLTHEDDVADAARYAR
                     RFGARRIIHRADVHAMPEAEVIIEGPEPRSYSEDFRIIPVPGHTAGSLCLLHRDRFLF
                     TGDHLWWEPEVRELASPRQLVWNAAALHRSIEQLTNCSFEWVLAGHGGRVWLPQSEMR
                     AALRDLVERRQ"
     gene            2733..3413
                     /locus_tag="PK614_03175"
     CDS             2733..3413
                     /locus_tag="PK614_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRB81240.1"
                     /translation="MGWMRALSWTGLLFMASISLAGVEPGHRTGDQALIAAAERNDVE
                     AVRQLLKEGASVRARDPQGRTALLAATYRNHVEIAKLLILTGSDVNTQDAMQNSPLLL
                     AGASGYLDILRMTLAADPDFAIYNRYGGTALIPACERGHVEVVKELLKTKIDVNHVNR
                     LGWTALLEAIILGDGGPTHQTIVGLLVAAGADLNLGDGDGVTPLRHARQNGYRVIADI
                     LLSAGARE"
     gene            complement(3441..3791)
                     /gene="uraH"
                     /locus_tag="PK614_03180"
     CDS             complement(3441..3791)
                     /gene="uraH"
                     /locus_tag="PK614_03180"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRB81241.1"
                     /translation="MAESRISTHVLDIAQGRPAQGIPVTLEGQGLAGIWKLLGKSRTD
                     ADGRSNDLYPAKQRLQPGVYRLTFDVSTYFRSQHVTSFYPEVVVIFTLSDAGQPYHIP
                     LLLSPFGYSTYRGS"
     gene            complement(3817..4731)
                     /locus_tag="PK614_03185"
     CDS             complement(3817..4731)
                     /locus_tag="PK614_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HRB81242.1"
                     /translation="MTLTELQYIVAVSRERHFGRAADRVFVTQPALSLAIKKLEDELG
                     VAIFERRKNHVELTPLGEQIVHQAQRVLEEADQIKLLAAQGKNQLVGALRFGVIATVG
                     PYLLPDLIPLLHKRAPGMPLEIEENLTVNLTAMLKSGKLDVIMIALPFEEPGILTQAL
                     YDEPFKAVVPADHRLAKRSKIDSALLSTERVLLPHAGHCFRQQVLESCPELSRSDTES
                     LQGNSLETIRQMVASGLGITVMPCSAVTAKHQNKRLAVLEFMKPVPERRIALAWRKGF
                     TRPAVIPVIQDAVGALKIPGLMAIASGQ"
     gene            4866..5099
                     /locus_tag="PK614_03190"
     CDS             4866..5099
                     /locus_tag="PK614_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81243.1"
                     /translation="MRHLGMVLVIGFGLGMLQGCATSSVQSSVPGATGRATATDERIK
                     DGTTDPVLLGIGSEQVRQYVERQVRPGTTLRFR"
     gene            5172..6248
                     /locus_tag="PK614_03195"
     CDS             5172..6248
                     /locus_tag="PK614_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB81244.1"
                     /translation="MRVARIEWAGVAGVGVIAGAMALLTATGVMGGANASQSGAVPVA
                     QAVPLGLEDPGSYIPSDNPQTARKIELGRWLFFDKRLSKNGTVACATCHMPGLAFTDG
                     QPVSTGIYRLQGGRSAPTAINRVYSKAQFWDGRADTLEDQSVGPFVSPVEHGFLDHNE
                     LVSKIKSIEGYRMLFQEVFGREVTVGDVGRAIAGFQRTLLSGNSPVDRFDMGGEETAL
                     SAAAQRGLELFRSKARCTRCHSGFNFSDEKFHNLGIGWDTNSVDLGRYMVTKNAEDIG
                     AFKTPTLREIARTAPYMHDGRFATLEDVVRFYNQGGIKNPHLDNTVIPLELTEPEQQD
                     LVAFLRSLNGEGWQQVAAPVEFPK"
     gene            6396..8021
                     /locus_tag="PK614_03200"
     CDS             6396..8021
                     /locus_tag="PK614_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81245.1"
                     /translation="MIAAASRWIDRNILELARDFQLSYLPPLMVYMAAGISGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWALKMPFGHLVDLLWRWKSLLVYFGAGMIAASL
                     LIMVGLIGHREAMVAVMPAEVWYVLSVLLSPIGYVIQDTVADAMTVEAVPRVDERGMP
                     IDEARLKLMHTTMQTLGRVAIISGGILVALINLYLFNGVESMPKEQVASIYEQVYLLA
                     LVIPAVSVLGVITASILRVRTRRRLLSQGMTLEQVASLTDRAAEPTKPNWWILGGSLA
                     FVLFTLTVGVADFAYSEEIVFAGSMAIVALLMVRLTSELAPDARHVLVGTALVVFVFR
                     AVPGPGEGATWWMIDKLKFDQQFLSVLSLIGSALTLAGMFLFRRFMAERSIAYVVGFL
                     TVAAFFLGLPIVSMYYGLHEWTAALTGGLVDARFIALVNTALESPLGQISMIPMLAWI
                     ANSAPANLKATYFAVMASFTNLALSLGQLGTKYLNELFVVTREVRDPATNLLQTPDDY
                     SQLGILLIVQALLGLALPFAAILFARFSRYRSA"
     gene            8042..8992
                     /locus_tag="PK614_03205"
     CDS             8042..8992
                     /locus_tag="PK614_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HRB81246.1"
                     /translation="MNTFQFLAEHGASVLFWVVFIEQIGFPIPAIPLLIAAGALVGAG
                     KMSLATALLVPVAAALPPDLAWYYLGRIKGGKVLGFLCRLSLEPDSCVRDTENLFHRY
                     GPRGLLLAKFIPGFNTVAPPLAGIVGMAATTFALYDVAGTLIWAAVSAGVGALFSDQL
                     EQLVGLLDQAGGLVFGVLLIGLVGFIGYKFYYRQQFLRHLRMSKISVDELKQRLDAGE
                     AISVVDVRHPLSLQLDPDTIPGAINFTLEEIEHRHQEIPRDRDIVLYCTCPNEVSSAR
                     TAFLLKKKGIHRVRPLEGGLDAWRERQYPVERRVNAGVGG"
     gene            9240..9854
                     /locus_tag="PK614_03210"
     CDS             9240..9854
                     /locus_tag="PK614_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81247.1"
                     /translation="MTRLVILMIAGTVLMSSGREAGAWPELSTTVSETMVWLTKGSSR
                     SKPAPLKYKVVGVFQRDKQFVGGSAVADVTKQVEFEFKWPLEDGVSPKVLPNIPTKAE
                     RYRDGSAVCPAPMLKGAYEHFELKSILVMGPMFGLVGERRTPDMDEYVGGGGCKGTHP
                     IQGQVREKQVLVTLPPEFLAGQLSKGQVFTQQDQEWTWTFTPIP"
     gene            10026..11072
                     /locus_tag="PK614_03215"
     CDS             10026..11072
                     /locus_tag="PK614_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HRB81248.1"
                     /translation="MKRKTEEIRRLILERIDDIGRVSQTEVAKTFGITRQAVFKNLKE
                     LVRNGVVQKTESEVHPYSLVPLKESHVTVPIGHETQEDQIWKESVAPLLADQKVNIRG
                     ICHYGFTEMFNNVIDHSGSETADVRITTDALVVRMVIQDHGVGIWKKLQQAFQLDDPR
                     HALLELTKGKLTTDPKRHTGEGIFFTSRMFDKFILASDKLTFCRFHEGNEWLFDVKEE
                     EAGPGTRVTMVLYLDSNKTTKEVFDKFTADLDDFGFSRTHLSVQLAKYEGDHLVSRSQ
                     AKRILNRLEKFREVYLDFKDVTEIGQAFADEIFRVFANDHPSVKFVPVNVTPDVSKMI
                     QRARSHQGQIELPM"
     gene            11262..11612
                     /gene="ssrA"
                     /locus_tag="PK614_03220"
     tmRNA           11262..11612
                     /gene="ssrA"
                     /locus_tag="PK614_03220"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11591..13276)
                     /locus_tag="PK614_03225"
     CDS             complement(11591..13276)
                     /locus_tag="PK614_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRB81249.1"
                     /translation="MRAAVYARKSTEQTGVAAEARSVTRQIENAKAYAESKGWTVSDE
                     HIYMDDGVSGAEFVKRPGFLRLMNALKPRPPFHVLVMSEESRLGREAIQVGYAFKQIV
                     DAGVRVFFYLTNQERTLDSATDKMMLALTNFASEFEREKASQRTHDAMMRKAKSLYVT
                     GNKIYGYDNQPVYGTDRNSDGTLRRQHVVRKINPEQSRIVAQIFEHYAAGCGLAAIAK
                     TLNQALVPPPMGGPLGWCPTAIREILRRDLYRGIVWWNRTQTIQRDGTKKQRKRPQSD
                     WLRLEAPELRIVSDAVWAQVEALRKRNAAAYKREGQGRFVSRPTGENQRSAYLLSSIA
                     KCGRCGGSIVAISKGRDGRSGQTVYRCSYFHKRGTSICRNAVSIKQNLLDSAVLSAMN
                     EAIDGRVLSASITRALEKLRAQQAHSPDRRSVVTRELSLLETRLQHLVELIASGHGTP
                     TVVQSLHKEEARKESLVAELAQLQALTTTLSLNERQITKRLREALGDHPALLGRHVPL
                     ARQMLRTLLDGHILCEPIEEDHRPGYRFTATGTFGRLLTGTSVINAGGGGEGS"
     gene            13359..13496
                     /locus_tag="PK614_03230"
     CDS             13359..13496
                     /locus_tag="PK614_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81250.1"
                     /translation="MGGLVFRHEDLGQYIHHGIKDLRRPGIRSMDSVVGLLPLNGIAS
                     A"
     gene            14124..14402
                     /locus_tag="PK614_03235"
     CDS             14124..14402
                     /locus_tag="PK614_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRB81251.1"
                     /translation="MPRVAVDDNKRMNLRVRPEQKATLVRAAALKHTDLTDFVLQPAL
                     REAKKVIEEAERIMLSERDSLMVLKLLEKPPAPNARLRKALASLPKAK"
     gene            14399..14926
                     /locus_tag="PK614_03240"
     CDS             14399..14926
                     /locus_tag="PK614_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB81252.1"
                     /translation="MSVPAWHEEPIGKHHDRKAFDCGDAAMNEFLHRYARQSHDAGGA
                     KTFLAIANADNTTVLGFYSLAPGALAYADTPEMVRHGLARHDVPGFRLARIATHVRVQ
                     GKGLGGQLLAAAGRRCLRAAADVGGVILIIDAKNDRAADWYAAYGAVPLANTPLTLVM
                     SLVTFATELKAKGRA"
BASE COUNT         3047 a         4143 c         4718 g         3203 t
ORIGIN      
        1 gcccgggagg ttgccgcagc gattcaggcc aagggtgggg tcgccgccgt catccaggcg
       61 gacatgagcc gggtggctga ggcgcaacgc ctggtgcagg agacggtaca ccagttccat
      121 cgcctcgata ttctggtcaa caatgcgggc cggttcattc caaagaagtt ggtggatacg
      181 acggagacgg agttcgacgc cattattgcg ctgaatgcga aggggccgta ctttgcgatg
      241 caggctgccg cgggggtgct cagtgatggt gggcggatcg tcaatatttc gtcggcgctc
      301 acccatctga aatttcccgg tgccacggcg cacctgggga gcaaggcggc gcttgaacag
      361 tatggcaagg gtctcgcgca ggaactggcg ccgcgcggga ttaccgtcaa tactgtgttg
      421 ccgggcttta ccgataccgg tgtgttgacg gagcagtatc gcgaaatggg cgaacagatg
      481 tcgccgttca agcggctcgg gctaccgtcc gatgtggccg acgtggtggc gtttttggtc
      541 agtgagcagg cacggtggct gaccggtcag gtgattcagg ccggcggcgg gttagtgatg
      601 taacgtgtaa aggaggattc atggcaaggc agaacgtttc gaccggtgga ccatgggaag
      661 cgaaaatcgg gtattcacgc gcggtgcgag tcggtcagtc ggtgcaggtc tccgggacga
      721 ccgcgatgac ggctacgggg cttgtcggca agggtgatcc ctacgcgcag acgatccagg
      781 ccttgaagac gattgaggcg gcgctcgtac aggccggcgc gtcgatagcg gatgtcgtgc
      841 gcacgcgcat ctacatggcg aatatcgatc aatggcagga ggtcggacgc gcgcatggag
      901 aggtcttcgg ctcgatccgg ccggcgacca ccatggtgga agtgaaacgg ttgatcgatc
      961 cggatatgtt gattgaaatc gaagcggacg cgatcgtgtc ctaaccgtat gcggaaaatg
     1021 tccgccagcg gcatgttcag gaaccggaaa acgcgagtcg tgaaaggtga aatgtcaggc
     1081 gccgtgcgac ctttgttccg gaaacgtgcg acctatttac gtgtcacgcc tcacgaatta
     1141 ctggatggcc tttctccacc atctgcagtt tggataggtg aagtcgtatg aaccagcagt
     1201 caccggaaat tgctcacctg tgtaagatcg atccggtgat gaagcgggtc atcggcgaag
     1261 tcggccctta tgcgttgtcg cttcgagcga ggcgttcccc gtttgagtcg ctggcccgcg
     1321 ccattgccta tcagcaactg catgacaagg cggcggagag tattctcagg cggttcatcg
     1381 cgctgttccc cggccggcgc tttccccgtc cggctgacct gctggcagtg acgccggagc
     1441 tcatacgagg cacaggtttc tctcgtgcca agattgcggc gctgcaggac ctcgcggcca
     1501 aggccattga gggaatcgtg ccgaccacga ccgtcattca acgactcgag gatgatgcca
     1561 ttgtcgaacg gttgatcgcg gtgcgcggcg tcggacggtg gaccgtggag atgctgctga
     1621 tttttcagct tggccgaccc gatgtcctgc cggtggatga ctttggcgtg cggaacggat
     1681 tccggatcgc atacaaacga aaggcgatgc cgactccaaa agagctgctg gtgtatggag
     1741 aacgctggag gccctatcgc acggccgcag cctggtatct ctggcgcgcg gcggatcggg
     1801 cgaaagtggt gcgaacaact ctgcccgtgg tgagggctgc ggggaagcga gctccgaatg
     1861 gctgacctgc gaaaaagact ggatgcgaat cgagccggca acttctatgt cgattccacc
     1921 tgcatcaatt gtgatgcctg ccgacaattg gctccgtcga gcttcgaaga agtgggagag
     1981 tattctgcgg tgactcatca gccggccacc gaaccggaga gaaggcaggc ctatcgcgcg
     2041 ttgctcgcct gcccgaccgg gtcgatcggg gccgagcata cgaaccaaac cctcttgagt
     2101 gccgcgaaag ccgacttccc gctcccgatt gaagggaacg tctactactg cggtttcaat
     2161 tcggaaaagt cgttcggggc gaacagtttc ttcgtgcagc atccggacgg caactggttg
     2221 atcgattcgc cgcggtatct caaaccgctt gtcgatgtct tcgatcggat gggaggcctc
     2281 gcacagatct ttttgaccca tgaagacgat gtggcggatg ccgccaggta tgcgcgccgg
     2341 ttcggagccc ggcggatcat ccaccgtgcg gatgtccatg cgatgcccga ggcggaggtg
     2401 atcattgaag gaccggaacc tcgctcgtac agcgaagact tccgcatcat ccccgttccc
     2461 ggccatacgg cgggcagcct ctgtctgctg catcgggacc ggtttctgtt taccggcgat
     2521 cacctctggt gggagccgga ggttagggaa cttgcctcgc cgcggcagtt ggtgtggaat
     2581 gccgcagcct tacaccgttc catcgagcaa ctgacgaact gttcattcga atgggtgttg
     2641 gccggtcatg gcggccgcgt ctggctgcca caatccgaaa tgcgggcggc gttgcgcgat
     2701 ctggtcgagc gacgacaatg aggtggaatc agatgggttg gatgcgcgct ctgtcatgga
     2761 ccggtctgct cttcatggcg agcatcagcc tggctggcgt cgagccgggt catcggacgg
     2821 gagaccaggc tctgattgcg gcggcagaac gcaacgatgt cgaggctgtg cgacagctgt
     2881 tgaaagaagg agccagcgtc cgggcccgtg atccgcaggg gcgcaccgcc ttgttggctg
     2941 caacctatcg gaaccatgtc gagatcgcga agctactgat cctgaccggc tccgatgtga
     3001 atacgcagga tgccatgcaa aacagtcctc tgctgttggc cggggccagc gggtatctcg
     3061 acattctgcg aatgacgttg gctgccgatc cggactttgc gatctacaat cgctacggag
     3121 gcaccgcgtt gattccggcc tgcgaacgag gccacgtgga agtcgtgaaa gaactgctga
     3181 agaccaagat cgacgtgaac cacgtgaacc ggttgggctg gacggctttg ttggaagcca
     3241 tcattctcgg tgacggagga ccgacacatc agaccatcgt cggattgctt gtggctgcag
     3301 gggccgatct caaccttggc gacggagacg gggtgacgcc gctccggcat gccaggcaga
     3361 acggctacag agtgatcgcc gacattctct tgtcagcggg ggcgcgggaa taaccggcag
     3421 agtggaccgt cgatcttctc tcaactccct cgataggtgc tgtagccgaa ggggctcagg
     3481 agcagcggaa tgtggtacgg ctgtcccgcg tcggacagag tgaagatgac gaccacctca
     3541 gggtaaaagc tcgtcacgtg ctgtgagcga aaataggtcg agacgtcaaa tgtgagacgg
     3601 tagactccgg gctgaagtcg ctgctttgcc ggatagagat cgttgctccg gccgtccgca
     3661 tccgttctgc tcttgccgag tagtttccag attcccgcca acccctggcc ttccaacgtg
     3721 acgggaatgc cctgtgcagg tcggccttgc gcaatgtcca gcacatgcgt gctgattcgt
     3781 gactcggcca tgcttcctcc tttcatcgtg ggacaattat tgtcctgagg caatggccat
     3841 cagtccggga attttcaatg caccgaccgc atcctgaatc acaggaatca ccgccggtct
     3901 tgtaaagccc ttcctccatg ccaacgcaat tctgcgttcc ggcaccggct tcatgaactc
     3961 caggacagcc agccgtttgt tctgatgttt ggccgtgacg gcactgcagg gcatgacggt
     4021 gatgcccagt ccggaggcca ccatctgacg gatcgtctcc agcgaattgc cctgcaaact
     4081 ttccgtgtcc gaccggctga gttccgggca gctctccagg acctgttgcc ggaaacaatg
     4141 tcctgcgtgc ggtaagagca cgcgctccgt gctcaagagt gccgaatcga ttttcgaccg
     4201 tttggccagc cggtggtcgg ccggcacgac ggccttgaac ggttcgtcat agagggcttg
     4261 ggtgagaatg cccggttctt cgaacggtag cgcgatcata atcacgtcca gcttgccgct
     4321 cttcagcatg gcggtcagat tgacggtcag attttcctcg atttcgagcg gcatgccggg
     4381 ggcgcgcttg tgcagcagcg ggatcaggtc gggcaagaga tacggcccca ccgtcgcaat
     4441 caccccgaac cgcaaggcgc caacgagttg gttcttcccc tgcgcggcga gcagtttgat
     4501 ctgatccgcc tcctccagca cgcgttgagc ctgatggacg atctgctccc cgagcggcgt
     4561 gagctcgacg tgattcttcc gtcgctcgaa aatcgccacc cccaattcat cttccagttt
     4621 tttaatggcc aggctgaggg ccggttgggt gacgaagacc cgatccgccg cccgtccgaa
     4681 atgacgctcg cgcgacaccg ccacgatgta ctgcagttcc gtcagggtca tgactcccct
     4741 cctctatcga taagctaagt ttatcgtgat gccatagact atcaattgga ctgattcgtc
     4801 ggcaaccgat atcgtatcag gcatggatcg acagcacaca agacaggaaa tcaaggaggt
     4861 gtgagatgcg acatcttggg atggtgcttg tcatcggatt cggactggga atgttgcagg
     4921 gctgcgcgac gtcatcggtg cagtcgtctg ttccgggagc aaccggcagg gcgacagcga
     4981 cggatgagcg tatcaaggac ggaacaacgg accctgttct gcttgggatc ggaagcgagc
     5041 aggtgcgtca gtacgtggaa cgccaggtac ggccgggcac gacgctccgg ttccggtgag
     5101 gcagagtggt cccgtggcat cgcagcccgg cagggcggag cgcaacggtt gaggtaagaa
     5161 aggaggatcc catgagggtc gcaagaatcg agtgggcggg agtcgcggga gtcggtgtga
     5221 ttgcgggagc catggcactg ttgacggcca ccggagtgat gggtggcgcg aacgcttccc
     5281 agtcgggagc cgtgccggtt gcgcaggcag ttcctttggg tcttgaagac cccggcagct
     5341 acatcccttc ggacaatccg cagacggcgc ggaagatcga gctggggcgc tggctctttt
     5401 tcgacaagcg actctcgaag aacgggacag tggcttgcgc aacctgtcac atgccgggcc
     5461 tggcattcac cgatgggcaa ccggtctcga cgggcattta tcgattgcag ggcggacgca
     5521 gcgccccgac ggcgatcaac cgggtgtata gcaaggcgca attctgggac ggtcgagccg
     5581 acacgttgga ggatcaatcg gtcggtccgt tcgtcagccc ggttgagcac gggtttctcg
     5641 accacaatga gttggtgagc aagatcaaga gcatcgaggg ctatcggatg ctctttcagg
     5701 aggtgttcgg ccgcgaggtg accgtcggcg atgtgggccg cgcgatcgcc ggttttcagc
     5761 ggacactgtt gtcgggcaac agcccggtgg accggttcga tatgggcggc gaggagacag
     5821 ccctgtcggc cgctgcccag cgggggcttg aactcttccg cagcaaagcc cgctgcacac
     5881 gttgccactc ggggttcaac ttctccgatg agaaatttca taacctcggc atcggatggg
     5941 atacgaacag cgtggatctg gggcggtaca tggtgacgaa gaatgccgag gatatcggtg
     6001 cgtttaaaac gcccacgttg cgagagatcg cgcgtacggc gccgtacatg cacgacggcc
     6061 ggtttgcgac gctcgaagat gtcgtccggt tctacaacca gggcggcatc aagaatccgc
     6121 atctcgataa caccgtgatc ccgctcgaac tgaccgaacc ggagcagcag gatctggtcg
     6181 cgttcttacg gtcgctcaac ggcgaagggt ggcaacaggt ggctgcgccc gtagagtttc
     6241 cgaagtaagc cgtacgttcg ccgagtggaa ggggcggggt caggaggccc cgccccttcc
     6301 cgccggctct tcgggctgct tgcaaatggt ggtcccgtac gggtaacaga gagggagata
     6361 acgatcagtt cactgccgtc cctggatacg cattcgtgat tgctgctgcc tcccgctgga
     6421 tcgaccgcaa tattctggaa ctcgcgcgcg acttccagct ctcctatctg ccgccgctca
     6481 tggtttatat ggccgccggg atttccggtc tgaccggcat cgtcggcacc ttcttcgtca
     6541 aagattatct gggcctgtcc gcggcctttc ttgccgcgtt gggattctgg gcgggcattc
     6601 cctgggcgct caaaatgccg ttcggacatc tggtcgatct gttgtggcgc tggaaaagtc
     6661 tccttgttta ttttggcgcc gggatgattg cggccagtct gttgatcatg gtcggcctga
     6721 tcggtcaccg cgaggcgatg gtcgccgtga tgccggccga agtctggtac gtgctcagcg
     6781 tgttgttgtc gccgatcggc tatgtgattc aggacacggt ggcggacgcg atgacggtcg
     6841 aggccgtgcc cagagtcgat gagcgaggga tgcccatcga tgaagccagg ctgaaactca
     6901 tgcacaccac gatgcagacg ctcgggcgtg tcgccattat cagcgggggt atcctggttg
     6961 ccctcatcaa tctgtacctg ttcaacggcg tggaaagtat gccgaaggag caggtggcct
     7021 cgatctatga acaggtgtat ctgctggcgt tggtgattcc ggccgtatcg gtgctcgggg
     7081 tgatcactgc gtcgatcttg agggtgcgaa cccggcggcg gcttctcagc cagggcatga
     7141 cgctcgaaca ggttgcctcg ctgacggacc gggccgctga accgaccaag cccaattggt
     7201 ggattctggg gggcagtctg gccttcgtgc tctttaccct gacggtcgga gtcgcagact
     7261 ttgcctacag tgaagaaatc gtgtttgccg ggtcgatggc catcgtcgcg ttattgatgg
     7321 tgcgcctcac cagcgagctg gctcccgacg caaggcatgt gctcgtgggc accgcgctgg
     7381 tggtgttcgt gtttcgtgcg gttcccggtc ccggcgaagg cgcgacctgg tggatgatcg
     7441 ataagttgaa gttcgatcaa cagttcctgt cggtgttgtc gcttatcggc agcgctctca
     7501 cgctcgcggg tatgtttctg ttccgccggt ttatggcgga acgatccatc gcctacgtgg
     7561 tcggctttct caccgtggca gcgtttttct taggtttgcc catcgtcagt atgtattacg
     7621 ggcttcatga gtggacggcg gccttgaccg gaggcctggt cgatgcgcgg ttcattgccc
     7681 tcgtgaacac ggcgttggaa tcgccgctcg gccagatttc catgatcccc atgctggcct
     7741 ggattgcgaa ctctgcgccg gcgaatctca aggcgaccta ttttgccgtg atggcgtcct
     7801 tcacgaacct cgcgctgtcg ctcggccaac tggggacgaa gtatctcaac gagctgtttg
     7861 tggtgacccg ggaggtgcgc gatccggcca ccaacctgct gcagacgcct gacgactaca
     7921 gtcagcttgg aatcttgctg attgtgcagg ccctgctcgg cctggccctc cccttcgccg
     7981 cgattctctt tgcgcgattc tcacgttatc gcagcgcctg agtgaacgga agaagatcgc
     8041 catgaacacc ttccaatttc tcgccgagca cggcgcctcg gtgctgttct gggtggtctt
     8101 cattgagcag atcggattcc cgattccggc gattcctctc ctcattgcgg caggtgcgct
     8161 cgtcggggcc ggaaaaatga gcctcgccac ggcgctgctg gtgccggttg ccgctgcgct
     8221 accgccggac ctcgcctggt actatcttgg tcgcatcaag ggcggcaagg tgctgggctt
     8281 cctctgtcgc ctgtcgctcg agccggactc ctgcgtcagg gatacggaaa atctgtttca
     8341 ccggtacggg ccacgtggac tgctgctggc caagttcatt cccggcttca acacggtggc
     8401 gccgccgctg gccgggattg tcggcatggc cgcgacgacc ttcgcactgt acgatgtcgc
     8461 gggcacgttg atctgggcgg cggtcagcgc cggagtcggc gcgttattca gcgatcaact
     8521 ggagcaactc gtcgggttgt tggatcaagc gggcggactg gtgttcgggg tgctgctcat
     8581 cggcctggtg ggattcatcg gctataagtt ctattatcgg cagcagttcc tcaggcatct
     8641 gcgcatgtcg aagatctccg ttgacgagct caaacagcgg ctggatgcgg gcgaggcgat
     8701 cagcgtggtg gatgtgcggc atcccctgtc tctgcaactc gatccggaca ccattcccgg
     8761 tgcgatcaac ttcacgttgg aagagatcga gcatcggcac caggagatcc cgcgcgatcg
     8821 cgacattgtg ctctattgca cctgcccgaa tgaagtctcc agcgccagga cggcgtttct
     8881 cttgaaaaag aaaggcatcc accgtgtgcg cccgctcgaa ggtggtctcg atgcctggcg
     8941 ggaacggcag tatcccgtag agcgacgggt gaatgccggt gtgggcggtt gacggcatgc
     9001 cgctgcgcgg aataagggag ggtcgctagg ggggtgtctc tagcccggat tattcatgaa
     9061 tgccgtcgcg aggcgttcga gaccgaagcc cgccggggct tctcgcaagt gaggctgacg
     9121 caggcgtact tgcagtccgt cgaggaggcc gaacgagaaa agccacgccg ggtgagagaa
     9181 tcggacgacg gagcaggtat tcatgaatag tccgggctag cttgcagagg agtgaacgca
     9241 tgacacgtct ggtgatactg atgatcgcag ggaccgtgct catgtcgagc ggccgtgagg
     9301 caggcgcatg gccggagctg tcgacgactg tgagcgagac catggtctgg ctcacgaagg
     9361 ggtcctcccg ttcgaagccc gcaccgttga agtacaaagt ggtgggagtc tttcaaaggg
     9421 acaagcagtt tgtcggtgga tcagccgtcg ccgacgtgac gaagcaggtc gagttcgaat
     9481 tcaagtggcc gctggaggat ggggtgtcgc ccaaagtgct gccgaatatt ccgacgaagg
     9541 cggagcgcta tcgcgatggc tccgcggtct gcccggcccc gatgctcaag ggggcctatg
     9601 agcattttga gttgaagagc attctggtga tgggaccgat gttcgggctg gtgggtgaac
     9661 ggagaactcc ggatatggac gaatatgtcg ggggtggcgg ctgcaagggc acccatccga
     9721 ttcaggggca ggtgcgcgag aagcaggtac tggtgacact cccgccggag ttcctcgcgg
     9781 gtcagttgtc gaaggggcag gtcttcacgc aacaggacca ggaatggacc tggaccttta
     9841 cgccgattcc gtgagtttcc gaggagaaag cttgggccac tcgtccacat cgtgtctcat
     9901 caggtagcta ctcgctgtag agcgaagcga agggtgatca tgcataggtt gacgcagaag
     9961 cgacaaaagt ttacgctatg actcggtaaa gtttacttgt tggcctatta aagtttacag
    10021 ctatcatgaa gagaaaaaca gaggaaatac gaagacttat attggaaagg attgatgaca
    10081 tcgggagggt ttcccagact gaggttgcca agacgttcgg aataactcgc caagctgtgt
    10141 ttaaaaatct gaaagaactg gtgaggaatg gtgtggtgca aaaaacagaa tcggaagttc
    10201 acccatatag cctcgtaccc cttaaagaat cacatgtgac cgtgccgatt gggcacgaga
    10261 cacaagagga tcaaatatgg aaggaatctg tcgcacccct gcttgcagat caaaaggtga
    10321 acatacgtgg gatttgtcat tatggtttta ccgagatgtt caataacgtc atagaccact
    10381 ctggatcgga gacggcggat gtccgcatca caacggacgc gctggttgtc agaatggtga
    10441 tccaagacca tggagtagga atttggaaga agttgcagca ggcgttccaa ctggatgatc
    10501 cgagacatgc gctattggag ctcacaaaag ggaagttgac gactgatcct aagcgacata
    10561 cgggggaagg catttttttt acctcccgca tgttcgataa attcattctt gcctccgaca
    10621 agcttacctt ctgtcgtttt cacgaaggca acgaatggct ttttgatgtc aaagaagagg
    10681 aagcgggccc aggtacaaga gttacgatgg tcctgtactt agattctaac aaaaccacca
    10741 aagaggtctt cgataagttc accgctgact tggatgattt tggattcagc aggacacatc
    10801 tgtctgttca gttggcaaag tacgaaggag accatctggt ctcgcgctct caggcgaagc
    10861 gcatcttgaa tcgactcgaa aagttcagag aggtgtatct cgacttcaaa gatgtgacgg
    10921 aaattggcca agcatttgcc gacgagattt ttagagtatt tgccaatgat catccctcag
    10981 taaaattcgt ccctgtgaac gtcactccag acgtttcaaa aatgattcaa agagctcgat
    11041 cccatcaagg acagatcgaa ttacctatgt gatcgctcgg agcttggcaa aaccgactta
    11101 gtgtgctggc atccccttga cctgtgctgt atgatgagca tctaagacgt aatgagacgt
    11161 gaaaagtgaa acgcgaaagg aagcggagaa gtagccgaat cctttcatga gcgaatggcc
    11221 tctcacgttt tacgtctgac gcttcacgat caccaagaaa ggggggcgac cggtttcgac
    11281 ggggatattg acgccatcgt cgcatgtcga gatctcggga actcgtaaaa ttccgggaaa
    11341 aacgcaactg ccaaccaaga actggcactc gcagcctaat ttaggctgag acgtcgttcc
    11401 atctgaggcc cgcgggggtg gaatggcgcg atgcagcggg ctggtcccac gccggtgctc
    11461 agcggcgtgg ggcgagacaa tactgggcta gcggtgattc taagccggcc gataggcgtg
    11521 ggtcgctgcg aagctaaaag aatcggctac acatgtagac gcgatgtgcc gacgatcttc
    11581 ggacaggggt tcaactcccc tcgcctccac caccagcgtt gatgacgctg gttccggtca
    11641 acagtcggcc aaatgtcccg gttgccgtga agcggtagcc gggcctgtgg tcttcctcaa
    11701 tgggttcaca gaggatgtgt ccatccagca acgttctcaa catctgacga gccaaaggca
    11761 cgtgcctccc cagtaacgca gggtgatcgc ccaaggcctc tcgtagtcgt ttggtaatct
    11821 gtcgttcatt caaagagagg gtcgtggtca gggcttgaag ctgcgcaagt tcggcgacaa
    11881 gactctcctt tcgcgcttct tccttatgaa gggactggac tacggtaggt gtcccgtgac
    11941 cgcttgcgat cagttccaca aggtgttgga ggcgcgtctc gaggagagac agttcccgag
    12001 tgacgaccga acgccggtcc ggagaatgag cttgctgtgc ccgcaacttt tccaatgctc
    12061 gtgtgattga cgctgaaagg actcgtccgt cgatggcctc attcatcgca ctcaggaccg
    12121 ccgaatcaag aaggttttgc ttaatggaga cggcattcct acagatcgac gttccacgct
    12181 tgtgaaagta ggaacatcgg tagaccgtct gcccggaccg cccatcacgg cctttactga
    12241 tagccacaat ggaacctccg cacctaccgc atttggcgat actactcagg agatacgcgc
    12301 tgcgctggtt ctcaccggtc gggcgtgaca caaaccttcc ctgcccctcg cgcttatacg
    12361 ccgcggcgtt tcgcttccga agggcttcaa cctgtgccca cacagcatcg gatacgatcc
    12421 gtaattctgg agcctccagt cgaagccagt cagactgcgg tcgcttgcgc tgcttcttgg
    12481 tgccgtctcg ctgaatagtc tgtgttcgat tccaccacac gatgccccgg tagagatctc
    12541 gccgcaggat ctctcggatg gctgtcggac accaaccgag aggtccgccc atcggcggcg
    12601 ggaccagtgc ttgattaagg gtcttcgcaa tagccgccag gccacaacct gccgcgtagt
    12661 gttcaaaaat ctgtgccacg atccgggatt gctccggatt aatcttcctc acgacatgtt
    12721 gacgcctgag tgtcccatcc gagttccgat cggtgccata caccggctga ttgtcgtatc
    12781 cgtaaatttt gtttccggtc acgtagagac tcttggcctt acgcatcatc gcgtcatggg
    12841 tccgttggct cgccttctcg cgttcaaact ctgacgcgaa attggtcaga gcgagcatca
    12901 tcttgtccgt ggcactgtcg agcgtccgtt cctggttcgt gagatagaag aaaactcgca
    12961 caccggcatc gacaatctgt ttgaaagcat acccgacttg gatggcctca cgacccagac
    13021 gcgactcttc cgacatcacg agaacatgga acggtgggcg gggtttcagc gcattcatca
    13081 gtcttagaaa cccggggcgt ttcacaaact cggcaccgct gaccccgtca tccatgtaaa
    13141 tgtgttcgtc ggaaaccgtc cagcccttag actcggcgta tgcttttgcg ttctcaattt
    13201 gtcgcgtcac ggagcgagct tccgctgcca cacccgtttg ttcggtagat tttctcgcgt
    13261 ataccgcagc gcgcatatta agctccttct ctctcttgag ggatcgaagg atgccgttga
    13321 ctctcggata atgtcaagcg tggagaggaa cctatggcat gggtggactg gtattccgcc
    13381 acgaggatct gggccagtat atccaccacg gtatcaaaga cctgcggcgg ccaggtatcc
    13441 gaagtatgga ctcggtggtg ggactcctgc cgcttaacgg catcgcgagt gcgtgatgaa
    13501 ggagtcatct tagagagcat gatcactcat tctttcgccg acaccgtgag cttccgagcc
    13561 gcacttatga gagggttcag aatttatgac cataccgaaa aggtagggga ggctggccaa
    13621 aagaaaggac catggggtcg ggtacgcctg agtcccgagg ggtcgctcac ccgggtctgc
    13681 agcaaggcgg agtgtcggac gagtttctac ggagacgtcc ctggcctggg ctgggcgagg
    13741 ggcttcggca gctacgcatc tgaggcaccg cgtccggaga cgccgtgcca agtggagtcc
    13801 ggcgccgtcg tgtcgcgcaa cgcgactcga ccgtgcggat gcgttagcga agacggagac
    13861 gttccttgag tcaaccattg ggccagccgt gaccgccaca ccccgcacgc gaaggctcag
    13921 accgcgtggg cgcgtggtta cgaggcttgt ttcatgcgct cgatcccacg gagaatgaag
    13981 acgcgcatga ggatctgata gggaatgcct tggtgtgcca ccagcgcctt cagtttttgg
    14041 agggtgagct catcgagggc ctcattgaca tacgcgcggt gttcaagtac gctttaaatg
    14101 agtacataaa gaggagagga gtcatgcccc gagtcgctgt agacgataat aaacggatga
    14161 atctccgggt gcggccggag cagaaggcca cgctcgtgcg ggcggcggcg ttgaaacata
    14221 cggacctaac ggattttgtg ctgcaaccgg ccttgcgcga agccaagaag gtgatcgaag
    14281 aagccgagcg catcatgtta tcggagcggg acagcctcat ggtgctgaag ctgctcgaaa
    14341 agccccctgc accgaatgcc cggctcagga aggctctcgc atcgttaccg aaagcaaaat
    14401 gagcgtcccg gcctggcacg aggagccgat cggcaaacac cacgaccgca aggcgtttga
    14461 ctgcggcgat gccgcgatga acgagttcct gcatcggtac gcccgccaaa gtcacgacgc
    14521 cggcggcgcg aaaaccttcc ttgcgatcgc caatgccgac aacacgacgg ttcttggctt
    14581 ctatagcctc gcgcccggcg cgctcgcgta tgcggacacg ccggagatgg tgaggcacgg
    14641 cctcgcgcgt cacgatgttc cgggctttcg gctcgcacgt atcgcgacac atgtgcgtgt
    14701 gcaagggaag ggtctaggcg gccagctcct ggctgcagca ggccgtcgct gcctgcgcgc
    14761 cgcggctgac gtgggcggcg tgatcctcat catcgatgcg aagaacgacc gcgccgccga
    14821 ctggtacgcc gcatacggcg ctgtgccctt agcgaatacg ccgctcacgc tcgttatgtc
    14881 ccttgtgacg tttgcaaccg aactgaaggc caagggtcgg gcataacacg tgtcagcacg
    14941 atgatgtgat agaggagtgc atgtcaggcc ttccatacct cctcgccatc cagtccatcc
    15001 gtcccttcaa actcagtctg gtctaagcat tgcgggatcg tctcatcgtt tttgacaccg
    15061 ctcgtctctg atactggccc attcttttgt caccatggtt gagcttcccc g
//
LOCUS       DAORQN010000027        14903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_260_length_14903_cov_8.008890, whole genome shotgun sequence.
ACCESSION   DAORQN010000027 DAORQN010000000
VERSION     DAORQN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_14903_cov_8.008890"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1837)
                     /gene="ilvD"
                     /locus_tag="PK614_03245"
     CDS             complement(164..1837)
                     /gene="ilvD"
                     /locus_tag="PK614_03245"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRB81253.1"
                     /translation="MTIDPRHKSHNLLDGPGRAPARAMLKAVGFTDADLERPLIGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALCRLNVPSLMIYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHAAGTMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGISPM
                     GRNGVPAMDQRKDDVAFECGKLVMDLLKKDIRPKQIITRRSIENAIAAVATTGGSTNA
                     VLHLLAVAREMGVRLSIDDFDKINRKVPLLADLKPGGRFTAADLYAAGGTTLVAKRLI
                     DAKILHPDQITVTGRTIGEEAKAATEKPDQQVLRPLSKPIKPTGGLVILKGNLAPEGC
                     VVKVAGHSILHFSGPAKVYEREEDAFKAVQAGKIKAGDVVVIRYEGPSGGPGMREMLG
                     VTAAIVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAIKGGPIAAVKTGDIITFDIT
                     KRRLDVNVTQKELASRLKKVKHPSPRYLSGVMGKYARHVSSASEGAVTT"
     gene            complement(2068..2427)
                     /locus_tag="PK614_03250"
     CDS             complement(2068..2427)
                     /locus_tag="PK614_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRB81254.1"
                     /translation="MIKHVAVPALMVAALCFTSTPAWSDKGHKGKDEKCDAAELVKDA
                     KVTIDQAIKTALDAVPGTAVEAELEKKHDKTVWEVEILGADGKMTEVHIDAGTGTIID
                     KEAKQEKHEKKGKKEKH"
     gene            complement(2460..3059)
                     /locus_tag="PK614_03255"
     CDS             complement(2460..3059)
                     /locus_tag="PK614_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRB81255.1"
                     /translation="MTTKLFTFATASAIACALTISPAWANPEGGYGGHSGGYEKGQHG
                     MGAAMMEMMMHGGAGHLIRHLLKHEKDIGLSADQVAKLKDLQLNLDKNRIKMEADIQV
                     AEREVKALNDDEKSDLSAIEAKLKQSADVQIGLRVLSIKTRREALALLTPEQRAKEQA
                     EHEKMMQQHKSAGQAAPHGKNPHSSNPHGANPHGEAKPQ"
     gene            complement(3085..3732)
                     /locus_tag="PK614_03260"
     CDS             complement(3085..3732)
                     /locus_tag="PK614_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRB81256.1"
                     /translation="MTIAEYALLAFSSLFVIVDPIAVVPAFLAMTPRDSVAQRLRTAR
                     VACLVSVALLTGFALFGQTVLKLLGITLPAVQIAGGLILLLVALDMLRAQRSTVQETA
                     AETAAGTNKDDIAITPLAIPMLAGPAAISTVILLETQATTFMLHVVLLGCLVLIGLAS
                     YSILAIGARSAKWLNPIAEKIISRLMGLLLAALAVQFMVNALTGDQGLLRGLTGH"
     gene            complement(3761..4792)
                     /locus_tag="PK614_03265"
     CDS             complement(3761..4792)
                     /locus_tag="PK614_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRB81257.1"
                     /translation="MATSQRGYYDILGVPRNATADDIKKAFRRRAREIHPDLHTGTKK
                     TEMEKKFKELNEAHEVLSDPDKRKKYDQYGSNWEQAEAYEQARQQAGAQAGRGGQAGG
                     FSGDFGDIFETFFGGRGRGGGTSPGFAVDGEDLETDVHLTIRDVLTGVSRRIDLTERV
                     VCKACGGSAIVRGRPCVVCGGAGTQAEKRTIEVRIPAGVQNETRVRLAGKGQPGINGG
                     KPGDLYLRVHIQANGVFRQKGFDIQVTLPVWPWEAALGAEVMAPTLTEPVKVKIPPGS
                     KADSKLRLKGKGLPTSTGEQGDLFLKLKIVMPTAISDEERALYEQLGRARHSDPRAEI
                     IAASRRSSS"
     gene            complement(4951..6051)
                     /gene="ugpC"
                     /locus_tag="PK614_03270"
     CDS             complement(4951..6051)
                     /gene="ugpC"
                     /locus_tag="PK614_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRB81258.1"
                     /translation="MAELELSNISKSFGDQPVLRDLSLQVPDGSFTILLGPSGCGKST
                     LLRIIAGLDSQTAGQVRIDGTVVDHLPPAERDIAMVFQQYALYPHLSVRENLAFALTI
                     RRTPRRVIDERIAEAAQLLDIEPLLDRKPKDLSGGQRQRVAMGRAIVRKPKLFLFDEP
                     LSNLDAQLRTSMRIELKKLQQRLQATMIYVTHDQVEAMTLGDRIVVIEGGRIRQADEP
                     HRTYAAPADPFVASFIGTPPMNLWEGALTAEDGKTVFKGNDLTFPLPARLAPSQSTQH
                     SAVTVGIRPEDISLRESPEAIQVTGLVELVEDLGADLLLHCRAGELRLVVRTARRTDK
                     IQGQTVKLFFPLDKLHLFIEHRRFDPPTTRPT"
     gene            complement(6204..7019)
                     /locus_tag="PK614_03275"
     CDS             complement(6204..7019)
                     /locus_tag="PK614_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRB81259.1"
                     /translation="MNRRILLALGILGTVGLSLLPFLWFVLTSFKSQGEIEAAPPAWW
                     PSGSLGFYRSALFDHHLFDYVLNSAVVAGSTTLLALLLAIPAAYALARLTIPGKQGIL
                     AVLLCVSMFPQMAIAGPVWRLLDTIGGLNHRWGVVLPYVALTLPLAIWILASFFKELP
                     PELEDAARVDGCGPWTTLFRITLPLATPGIFTAAILILIYAWNEFFFALLILTQPEQQ
                     TLPVGIALFQGEFTMPWGELAAASVVATLPLIVVVLLCQRWIVSGLSAGAVKG"
     gene            complement(7016..>7880)
                     /locus_tag="PK614_03280"
     CDS             complement(7016..>7880)
                     /locus_tag="PK614_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRB81260.1"
                     /translation="WTMVAPALLVTMVFALYPVLDSLWLSLHNIFIGLPQLGSPFVGL
                     DNYVALLRDPVAQQALVVTLAFVGLSTILELACGLIIALVIHERFRGRGLVRAAILIP
                     WAIPTVVASQLWRYIFNDQYGFANLLLFGERVTDYVPWLAYPGVAFGIVVLADVWKTS
                     SFAALLILAGLQVIPDDLYDAARVDGATVWQRFWHITLPLLKPALLLALLFRTMDAFR
                     VFDLVFVMTQGGPGDATQVLQFYGYQTLFTEGRIGYGSAVSVVVFLMILALSLTYLRA
                     IGSSLLERRRT"
     assembly_gap    7881..7890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7922..9298)
                     /locus_tag="PK614_03285"
     CDS             complement(7922..9298)
                     /locus_tag="PK614_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB81261.1"
                     /translation="MRWITPRQHIQRPIDSPSQRVFRWITLVSVLFISFGVDIHHARS
                     APGSDPATQTPRVTLRFVSWKPDHPRVWDEALAEFTKTHPDISVVRELAPHSSTAYHD
                     LLTQKLKNRDATVDVFFMDVIWVPEFAEAGWARRLDERFTPAMREEFLPATIEVGRYS
                     DHLYGVPSRIDAGLLYYRSDLLAKYGFSPPTTWDELARQADAIVAGERRNNPTLRGYT
                     AQFKQYEGLVCNLLEFIDSHGGSLLTSDGTHSTLGRPETLAAVQFVRDRVIGRLASRA
                     ALTYQEPESLSVFLQGHAVFHRNWPYAWELANNRTRSTIAGQVAVMPLPGFTPGRTAA
                     ALGGWLYGISAYSQHPDEAWALIEFLSSRAMQKKFTQEAGIAPSRQALFSDPDLLAAA
                     PQLRNHFAVLRSATARPRSPLYPAISHLLQRYFSRALAIDDLDLAQEAAVTDAHIDRL
                     LALTRAAP"
     gene            complement(9433..9843)
                     /locus_tag="PK614_03290"
     CDS             complement(9433..9843)
                     /locus_tag="PK614_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB81262.1"
                     /translation="MKTPFTLGAVWHFGLAVRDPRASATWFMRILGLSKQFEYEGGVG
                     IGNANVLIVFSKGKPSPKTIGHVAFHLPNMAALKKALAHLKKHRVEVEDPGDEIGSVA
                     PGSKSMGLWFSDPDGYRWELAVQSKKQPPGLAVD"
     gene            complement(9840..10442)
                     /gene="ogt"
                     /locus_tag="PK614_03295"
     CDS             complement(9840..10442)
                     /gene="ogt"
                     /locus_tag="PK614_03295"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF007626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRB81263.1"
                     /translation="MAYNLCLFRETLETPIGTLVVITDERGDLRAIDWTDYEDRMRRL
                     LVRHYGSDRMTMTPAIQLSRPALAIAAYFEGALSAIDPLSTATGGTPFQKRVWQALRE
                     IPAGQTMSYGQLARRIGQPTAVRAVGLANGANPIGIVVPCHRVIGANGSLTGYGGGLD
                     RKRWLLNHERQATESATRFSAATTETQWYDHSLLDQEVQT"
     gene            complement(10432..11940)
                     /locus_tag="PK614_03300"
     CDS             complement(10432..11940)
                     /locus_tag="PK614_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HRB81264.1"
                     /translation="MEPLDPDISYRALQTRDPRFDGRLFVGVVSTGIYCRPICPARTP
                     KREHCRFFANSASAQAAGFRACLRCRPEIAPELAGWRGTSNTVSRALALIAEGFLDRE
                     DGTVDALATHVGMGARQLRRLFDQHLGVAPVVVAQTRRLLFAKQLLHDTRLSMTDVAI
                     AGGFGSVRRFNHVFHTLYHTPPGELRRCSAQPSSTLPADAPVVLQLPYQQPYDWQNML
                     DYLRARAIDGLEVVQDGLYRRSVLLDGLQGTVTVAHNPARHCLLATIRFPSVRALQAI
                     VARVRHQFDVGADVDSIATHLSKDGALAPLIALRPGLRVPGCWDGFELAVRAVLGQQI
                     TVEAARRLGGILVDLWGDRLLNSGEIRLTRTFPTASRLATADLSRLGMPAARRATIKA
                     LAHAAVANPRLFLPGKSLDEQIARLRAIDGIGAWTAHYIALRALREPDAFPAADVGLL
                     RGAAQLEGVVVTAAQLLHRAEGWRPWRAYAAQYLWTITTRTSHAERKIRHGL"
     gene            12439..13923
                     /locus_tag="PK614_03305"
     CDS             12439..13923
                     /locus_tag="PK614_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="insecticidal delta-endotoxin Cry8Ea1 family
                     protein"
                     /protein_id="HRB81265.1"
                     /translation="MSTVLQPPTHSAPVPTDITDWSNTAMNLIASTLGLIPEVGSLLS
                     GLVYILWPSDQEDVWDEIKDQVQALVQQDLTQLVENLEVQTLQGLKGSIQDYQDALTT
                     NDPSNISQNWTSAKLNFVQQMPQFQTQGYEVALLPYYAQAVNLYLALLRDGVLHGKDW
                     GWTDADVAVTTKEMNQATQDVCQWVDTTFGQEYGRRTSAFGAAQDAYLQFCNSGNDPV
                     NYSLGYHYNVTAWNKQNNFLTWMTLKALDFRNLWPFFNSGQVDVVLHREIYSMAFGSS
                     DYSPIPIRPWWSLNPPAPTQPLTTIDVFAADRINGVRLNYPSGGGPGGETQVVCGALT
                     DGCVTVYADADPIVEVDIYAGDIVSALSFTGQDGSSTGQIGGNKPPARSPIGFIFREN
                     SILSSLYIHGTSEFYQSADLIIVGYQYQQKATTDLTALRHLYVCSPSQIDLTELAARC
                     VTKPVSIDALKAAADTEGWDAQRQQYRNSLTRRSTAANRPRRAS"
     gene            13920..>14903
                     /locus_tag="PK614_03310"
     CDS             13920..>14903
                     /locus_tag="PK614_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81266.1"
                     /translation="MTTVTSPEALSIHKLLQIPASFAWNFDPGHLATIGCSGDIDGDG
                     RDEFIVSSTQATSPVACLSVLSYVQPNELDPGWSGNQQPMLIVQWSSSAGIPNWMYPP
                     SPAPQSCSNFSFWSADVDGDGIQEIIAFFPGYTGASASLGVLKWDGHSLSCVWQTFGV
                     ISGIPGTHSCPLAPGLQLYPMHSPAGEGGDRILFVQPVSGLGTLVGLMEWNAGAFSSV
                     WLNNGSIPGAGKVPAWTLGPVDQFAVADVDGDTEDELILLVPSYADKVNQLNPALAVA
                     KWSGGVLSVIWHVSSSAGNAPDYVTQSASLLVADLDGTGVAKVIIPMAEYKG"
BASE COUNT         2875 a         4341 c         4687 g         2990 t
ORIGIN      
        1 gcgcgcaaaa accccccccc ccctagcttt gggggggggc gccgcgccaa tgggcccccg
       61 gggggggggg gggcccccgg gggggcgcgg ggggggcccc caacgggccg ggggggggcc
      121 ccgccccccc ccgcgagacc ccatcgagac cgttttacca tggctaggtg gtcaccgccc
      181 cttcggaggc ggaagagacg tggcgggcat atttccccat gacgccggac aggtaccggg
      241 gggacggatg cttcaccttc ttcagacgcg aggccagttc cttctgagtc acattgacgt
      301 cgagacgtcg cttggtgatg tcgaaggtga tgatgtcgcc ggtctttacc gccgcgatcg
      361 gaccaccctt gatggcttcc ggcgcgacat gtcccgccat caggccgtgg gtggctccgg
      421 agaatcgtcc gtcggtaagc agcgcgacgg agtcgccgag tcctgctccg acgatggcgg
      481 ccgtcacgcc gagcatttcc cgcatgcccg ggcctcccga cggtccctca tatcgaatca
      541 cgaccacatc accggccttg atcttaccgg cctgcaccgc cttgaaagca tcttcctcgc
      601 gctcatagac cttggccggt ccgctgaaat gcagaatgga gtggccggcg actttcacga
      661 cgcacccttc cggagcgagg ttgcccttga ggatcaccag acctcccgtc ggcttgatgg
      721 gcttggacag gggacgcagc acctgttggt ccggcttctc cgtggcggcc ttagcttcct
      781 caccgatcgt gcgtcccgtg accgtgatct gatccggatg caggatcttc gcatcgatca
      841 aacgtttggc gaccagggtt gtgccaccgg cggcatacaa gtccgccgcg gtgaatcggc
      901 cgccaggttt gagatcggcc agcaacggca ccttccggtt gatcttgtcg aaatcgtcga
      961 tgctgagccg cacgcccatt tcccgcgcca cggccaacag gtgcaagacg gcattcgtcg
     1021 atccaccggt cgtcgcgacg gccgcgatcg cattttcgat ggagcggcgc gtgatgatct
     1081 gcttgggccg aatgtctttc ttgagcaggt ccatgaccag tttgccgcat tcgaacgcca
     1141 catcgtcctt acgctgatcc atggccggga cgccgtttcg gcccatcggc gagatgccga
     1201 gaaattcgaa ggcgatggcc atggtgttgg cggtgaactg gccgccgcag gcaccggggc
     1261 ctggacaggc atggtcttcg agatccttga gttccgcatt cgtcatggtg ccggccgcat
     1321 gtttgccgac cgcttcgaac acgtcttgaa tcgtgacgtc gtgtccctgg aactgtcccg
     1381 gcatgatgga gccgccgtag atcatgagtg acgggacgtt caagcggcag agggccatga
     1441 cggtgccggg aatcgtcttg tcgcaaccgg acagggcgac gactccgtcg aacaagtgtc
     1501 cacgtgcgac cagttcgatg gagtcggcaa tgacttcccg gctgatgagc gaggccttca
     1561 tgccttcggt gcccatggaa atgccgtccg acaccgcgat ggtgttgtat tcgatcgggg
     1621 tgccgcctgc cgcgcgaatt ccggccttca cccgttccga taaacggcgg aggtggaaat
     1681 tgcagggcat gacctcgatc caggtgttgg cgacgccgat caaagggcgt tcgaggtcgg
     1741 catcggtgaa gccgacggcc ttgagcatgg cgcgggccgg tgcgcggcct ggtccgtcga
     1801 gcaagttgtg gcttttgtgt cgtgggtcga tcgtcatgga aactccttca tttacgggaa
     1861 cggggggtta ccgcttcgca cagcgcgcgt tctgatggat cgcaaacttt cgcatagata
     1921 ggctcagagg gtcaatccgc cggtcctgag atcggctgcc gctaggcttg ggaatggcaa
     1981 gcagggggaa aggggggtag atgaatggga cgggtcgaga cggtgcggca ggatcgcatc
     2041 tgaaggcgcg accctgccgc aggaacctta gtgcttttct ttcttgccct tcttctcgtg
     2101 cttctcttgt ttggcttcct tatcgatgat ggtgccggtg cccgcgtcga tatggacctc
     2161 cgtcattttg ccgtcggctc ccagtatttc gacttcccag accgtcttgt cgtgcttctt
     2221 ttcaagctcg gcttcgacgg ccgtgcctgg aaccgcatcc aacgcggtct tgatggcctg
     2281 gtcgatggtg accttggcat ccttcaccaa ctcggcggcg tcacacttct cgtccttgcc
     2341 tttgtgtccc ttgtcactcc aggcaggagt gctcgtgaaa caaagtgccg cgaccatcag
     2401 ggcgggtact gcaacgtgct tgatcatgtg tgacctccgg ttctgtgcaa cgtgcacggt
     2461 tattggggct tcgcctcacc gtgtgggttg gcgccgtgcg gattgctgct atgcggattc
     2521 ttgccgtggg gagcggcctg gcccgcgctc ttgtgctgct gcatcatctt ctcatgttcg
     2581 gcttgctcct tggcccgctg ttccggggtc agcaacgcga gggcttcccg tctggtcttg
     2641 atcgacagga cgcggagtcc gatctgcacg tcggcgcttt gcttgagctt ggcttcgatg
     2701 gcggacaagt ccgacttctc atcgtcgttc agtgccttca cttcccgttc ggcgacctgg
     2761 atgtcggctt ccatcttgat ccggttttta tcgaggttga gctggaggtc cttgagcttg
     2821 gcgacctggt cggcgctcaa tccgatgtcc ttttcatgct tcaggaggtg gcgaatcagg
     2881 tgcccggcgc cgccgtgcat catcatttcc atcatcgcgg cgcccatgcc gtgctggccc
     2941 ttctcgtagc cgccgctgtg tccgccatat ccaccttcgg ggttcgccca ggcgggggag
     3001 atcgtcagcg cgcaggcgat ggccgaagcg gtggcaaatg tgaacagttt cgtcgtcatt
     3061 cgttcctcct tcagttgaga gcgattaatg tccggtcaac ccgcgtaaca gcccttggtc
     3121 cccggtgagg gcattcacca taaattgaac ggccagcgcg gcgagcaaca agcccatgag
     3181 ccgcgagatg atcttctcgg cgatgggatt gagccacttc gcgctgcgtg cgccgatcgc
     3241 taatatactg taactcgcca acccgatgag tacaaggcat cccaataata cgacgtgcaa
     3301 catgaatgtg gtggcctgcg tctccagtag gatgaccgtc gagatcgcgg ctggccccgc
     3361 cagcatgggg atggcgagcg gggtgatggc gatgtcgtcc ttgttggtgc cggccgctgt
     3421 ttccgcagcc gtttcctgca ccgtggaccg ttgcgcccgc aacatgtcca acgcgacaag
     3481 taataggatc agcccgccgg caatctgaac cgccggcaag gtaatgccta acaatttgag
     3541 gacggtttgc ccgaacaggg cgaatcccgt gaggagggcg acgctaacca gacaggcgac
     3601 ccgcgcggtc cggagccgtt gcgccacgga atcccgtggg gtcatggcga ggaaggccgg
     3661 gaccacggcg atgggatcga cgatcacgaa aagcgaactg aacgcgagga gcgcgtattc
     3721 ggcgatagtc atagaaggga ttggcctggg gcggcgctac tcaggatgaa ctgcgtctcg
     3781 atgcggcgat gatctcggcc cggggatcgc tgtggcgggc gcggcccaat tgttcgtaga
     3841 gcgcccgttc ttcgtcagag atggcggtgg gcatgacgat tttcagcttc aggaagagat
     3901 ctccctgctc gccggtcgag gtcgggagcc ccttgccctt gaggcgcagt ttactgtcgg
     3961 ctttgctacc ggggggaatt ttcaccttca ccggttccgt cagggtcgga gccatcactt
     4021 cagcgcccag cgccgcctcc catggccaga caggaagggt gacctgaata tcgaatccct
     4081 tttgccgaaa gacgccgttg gcctggatat ggacgcgtag atagaggtct cccggtttgc
     4141 ctccattgat gccgggttgt cccttgcccg ccaggcggac acgggtctca ttctgcacac
     4201 cggccggaat gcgtacttcg atcgtgcgtt tttcagcttg ggtgccggca ccaccgcaca
     4261 cgacacaggg ccggccccgt acgatggcgc tgccaccgca ggccttgcat acgacgcgtt
     4321 cggtgagatc gatgcgccgg ctgaccccgg tcaggacgtc gcggatggtg agatggacat
     4381 cggtttccag atcttcccca tcgacggcaa atcctggcga ggttccccca ccacggcccc
     4441 gtccgccgaa aaaggtctcg aagatatcgc cgaagtcgcc gctgaatccc ccggcctggc
     4501 cgccacgtcc cgcttgggcg ccggcctgtt ggcgcgcctg ctcataggct tccgcctgtt
     4561 cccagttcga gccgtactgg tcgtattttt ttcgcttgtc cgggtccgac agcacctcat
     4621 gcgcttcgtt caattctttg aacttcttct ccatctccgt tttttttgtg ccggtatgga
     4681 gatcgggatg gatttcgcgg gcgcgccgcc gaaaggcctt cttgatgtcg tcggccgtcg
     4741 cgtttcgcgg cacgccgagg atgtcgtagt aaccgcgttg agatgtggcc atcagggtct
     4801 atccgtagaa tttattcggt tgcgcgtagg ggaagaatgc aatcctttcc ggagcttacg
     4861 aggtctccgt acgcattgca agcgaactca tggtttttct cttccgccgc cgttgtcctt
     4921 atgaaagaga taagagagcc cgggggacag tcaagtgggc ctggtggtag gcggatcgaa
     4981 tcggcggtgc tcgatgaaga gatgcagttt atcgagagga aaaaagagtt tcactgtttg
     5041 tccctgaatt ttgtcggtcc ggcgggccgt acgcaccacg agtcgaagct caccggcccg
     5101 gcaatgcaga agcaggtccg ccccgagatc ctccaccagt tccaccagtc ctgttacctg
     5161 tatggcttcc ggcgactccc ggagcgagat gtcttccggg cggatgccga ctgtcacggc
     5221 ggagtgttgt gttgactgag atggggccag ccttgccggg agcgggaagg tcaggtcgtt
     5281 gcctttaaag acggtctttc cgtcttctgc cgtgagggcc ccttcccaga ggttcatcgg
     5341 cggtgtcccg atgaaggagg cgacgaaggg atcggctggt gcggcatagg tgcgatgggg
     5401 ctcatcagct tggcggatgc ggccgccctc gatgaccacg atgcggtcgc ccagcgtcat
     5461 ggcttccact tgatcgtggg tgacatagat catggtggcc tgcaggcgct gctgcagctt
     5521 tttgagttcg atacgcatcg aggtgcgcag ttgtgcatcc aggtttgata gtggttcatc
     5581 aaacaggaac agcttcggtt tccgcacgat cgcccgcccc atcgcgaccc gttgccgttg
     5641 tcctcccgac agatctttgg gtttgcggtc caggagaggc tcgatgtcca agagttgcgc
     5701 ggcttcggcg atgcgctcgt cgatcacccg gcgtggagtt cgtcggatcg tcagggcaaa
     5761 ggcgagattc tcgcggaccg aaaggtgcgg gtagagcgcg tactgctgaa agaccatggc
     5821 gatgtcgcgt tcggctgggg ggagatgatc gactaccgta ccgtcgatgc gcacctggcc
     5881 tgctgtctgg ctatcgagcc cggcgatgat gcgcaggagc gtcgatttgc cgcaaccgga
     5941 ggggccgagc agaatcgtga agctcccatc cggaacctgc agtgagagat cgcgcagcac
     6001 gggttggtca ccaaacgatt ttgagatgtt actcagttct agttcagcca caggacaccc
     6061 taccagaaac agtgctcaca gaatgttcag aaaggctgtc cagcaaggcc gcaggaagca
     6121 cggtgactga ggcgtaccct tcgggtacgt cgtaggaaga cgtgcgacga gaacgccgct
     6181 ggcagatctt tttcaacatt ctgctagcct tttaccgccc cggcggacaa tccgctcaca
     6241 atccatcgtt gacatagcag cacgaccacg attagtggaa gggtcgccac cacggaggct
     6301 gcggccagct ctccccaggg catggtgaat tctccctgga agagagcgat gccgacagga
     6361 agggtctgtt gctccggctg ggtcaggatc aggagggcga agaaaaactc gttccaggca
     6421 tagatcaaga tcagaatcgc ggcggtgaaa atccccgggg ttgccagcgg aagcgtgatc
     6481 cggaacagag ttgtccaggg cccgcaacca tccactcgtg ccgcatcttc caactccggc
     6541 ggcaattcct tgaagaagct ggccagaatc cagatcgcca acggcagggt gagggccaca
     6601 tagggcagca cgacgcccca gcggtggttg agcccgccga ttgtgtcgag cagtcgccag
     6661 acgggcccgg cgatggccat ttgagggaac atcgaaacgc agagcagcac ggccagaatg
     6721 ccttgtttgc cggggatggt cagccgggcc agcgcgtagg ctgccgggat ggcgagcaaa
     6781 agggcgagca gcgtggtgga tccggccacg acggcgctgt tcagcacata gtcgaagaga
     6841 tgatggtcga agagcgctga gcgatagaat cccaggctgc ctgagggcca ccaggcgggc
     6901 ggcgcggctt cgatctcacc ttgcgacttg aacgacgtga ggacaaacca gaggaacggc
     6961 agcagactga gtcccaccgt accgaggatg ccgagtgcga gaaggatccg acgattcatg
     7021 ttcgcctgcg ctccaagaga ctcgagccga tggcgcgcag gtacgtcaac gacagtgcca
     7081 ggatcatcag gaacacgacc acggagacgg cagagccgta tccgatgcgg ccttccgtga
     7141 agagggtttg gtagccatag aactgcagga cctgcgtggc gtcgcccggg ccgccttggg
     7201 tcatcacgaa caccagatcg aagacacgaa acgcatccat ggtccgaaat aagagcgcga
     7261 gcaacagtgc cggtttcagc agcggcaggg tgatgtgcca aaaccgctgc cagacggtgg
     7321 ccccatccac acgcgccgcg tcatagagat cgtcgggaat gacctggagg ccggccagga
     7381 tcagcagggc ggcgaaggag gaggttttcc agacatcggc cagcaccacg atgccgaagg
     7441 ccacgcccgg ataggccagc caggggacgt agtcggtgac gcgttcgccg aacaacaata
     7501 agtttgcgaa gccatactgg tcgttgaaga tatagcgcca cagttgcgac gcgacgactg
     7561 tgggaatggc ccagggaatc aagatcgcgg cccgcacgag accgcgcccg cgaaatcgct
     7621 catgaatcac cagcgcgatg atcagcccgc aggccaattc gagtatcgtg gagaggccca
     7681 caaaggcaag ggtgacgact agtgcctgtt gcgccaccgg atcacgaagc agcgccacat
     7741 aattgtccag cccgacgaac ggactgccga gctgcggcag cccgatgaaa atattgtgca
     7801 ggctgagcca gagcgaatcc aacacggggt acagtgcgaa gaccattgtt acgagcagcg
     7861 ccggtgccac catcgtccag nnnnnnnnnn tcgcgatttc gcaggattga ggcggcgcgg
     7921 atcatggcgc agccctcgtg agggcgagta gccgatcgat gtgagcgtcg gtcacggccg
     7981 cttcctgcgc aaggtcgagg tcgtcgatgg caagggcacg gctgaaataa cgctgtagta
     8041 gatgcgagat cgcggggtac aggggggaac gcgggcgagc ggttgcggac cggaggaccg
     8101 cgaagtggtt acggagttgc ggcgctgctg ccagcaggtc gggatcggaa aacagcgcct
     8161 gacgggaggg cgcgatgccg gcctcctggg tgaatttctt ttgcatggcc cggctggaga
     8221 gaaactcgat gagcgcccag gcctcgtctg gatgctgcga gtaggcgctg atgccgtaga
     8281 gccagccgcc gagtgctgcg gccgtgcgcc ccggagtgaa cccgggaagg ggcatcaccg
     8341 cgacctgtcc ggcgatggtc gagcgagtcc ggttgttggc cagttcccag gcatagggcc
     8401 agttgcggtg aaagactgca tggccttgaa gaaagacgga gagggattcc ggttcttgat
     8461 aggtcaacgc cgcgcgtgat gcaagccggc cgatcacccg gtcacgaacg aactgcacgg
     8521 cggccagcgt ttcgggtctg cccagagtgg aatgagttcc atccgacgtc agcagactcc
     8581 cgccgtggct gtcgatgaac tccagcaggt tacagacgag gccttcatac tgtttgaatt
     8641 gtgctgtgta gccgcgcagg gtcggattgt tccgtcgttc cccggcgacg atggcgtcag
     8701 cttgtcgggc gagctcgtcc caggtcgtcg gtggtgagaa gccatacttg gccagcaaat
     8761 ccgagcggta gtagagcagc ccggcatcga tgcgactcgg gacgccgtag agatggtcgg
     8821 agtatcgacc cacctcgatg gtggcgggga gaaactcctc acgcatggct ggagtaaatc
     8881 gctcgtcgag ccgtcgggcc caaccggctt cggcaaactc cggcacccag atgacatcca
     8941 tgaaaaagac atcgaccgtg gcatcacgat tcttcagctt ctgcgtcagg agatcgtgat
     9001 aggccgtcga gctgtgcggg gccagttcgc gcacgaccga aatatcagga tgggttttgg
     9061 tgaactcggc gagggcctcg tcccatacgc gcgggtgatc gggcttccag gagacaaacc
     9121 gaagcgtaac acggggcgtt tgcgtggccg ggtctgatcc aggcgccgat cgggcgtgat
     9181 ggatatctac cccgaagctg atgaacagaa cgctaaccag cgtgatccag cggaataccc
     9241 gttgtgaagg ggaatcaatc gggcgctgga tgtgctgacg tggggtgatc catcgcatca
     9301 ctccataaag ttatacacag tcagggtggg tgatgcaaaa ttagcacaat gcaagcagac
     9361 tcgtggcgct tgagggcagc gggtgtttcc cctcagcgcc gccgagctaa taggtttcct
     9421 ccaggctcaa tttcaatcga ctgccagccc cggcggctgc ttcttgctct gcacggccag
     9481 ttcccaccgg tacccatcgg ggtccgaaaa ccagagtccc atgctttttg aaccgggagc
     9541 gaccgatccg atctcatctc ccggatcctc tacttcaacc cgatgttttt tgagatgggc
     9601 tagggctttt ttgagtgcag ccatgttcgg cagatggaac gccacatgcc cgatggtttt
     9661 cggcgagggt tttccttttg agaagacgat cagcacgttg gcattgccga ttcccacgcc
     9721 gccttcatat tcaaattgtt tgctgagtcc caatatccgc atgaaccatg tggcgctggc
     9781 gcgcggatcg cgaactgcca ggccaaaatg ccagactgcg ccgagggtga atggagtctt
     9841 catgtctgca cctcctggtc gagaagagaa tgatcatacc attgggtctc tgttgtagct
     9901 gcagagaagc gcgtggcgct ttcagtcgcc tgtctctcgt gattcagcaa ccaccgcttg
     9961 cggtccagtc ctccgccgta gccggtcaac gagccattag ccccgatcac tcgatgacag
    10021 ggcaccacga tgccgatggg gttggcgcca ttggccaggc ccacggcgcg caccgccgtc
    10081 ggttgtccga tacgtctcgc gagctgccca taactcatgg tctgtcctgc agggatctcg
    10141 cgcagtgcct gccagacgcg tttctgaaac ggggtgcccc ctgtcgccgt cgagagaggg
    10201 tcgattgcag agagcgctcc ctcgaagtag gccgcgatgg ccaacgcagg tcgtgataat
    10261 tgtattgccg gtgtcatggt catccggtcg cttccatagt gccggacgag cagccggcgc
    10321 atgcgatcct catagtccgt ccaatcgatg gcgcgcaaat ctccgcgttc atctgtgatc
    10381 accaccagcg ttccgattgg tgtctccagc gtctctctga aaagacacaa gttataagcc
    10441 atggcgaatc ttcctctctg cgtggctggt gcgcgtggta atggtccaga gatactgcgc
    10501 ggcataggcc cgccaggggc gccaaccctc cgcgcggtgc agcagttgag cagccgtgac
    10561 gacgactcct tccagttgtg cggcgccgcg cagaagaccg acatcggcgg ccggaaaggc
    10621 atccggctcg cgcagggcgc gaagggcgat atagtgagcg gtccacgcac cgataccatc
    10681 gatcgcccgg agtcgcgcga tctgttcgtc gagcgatttg cccggcagga acagtcgagg
    10741 attggccacg gctgcgtggg ccagggcttt gattgtggcc cgccgagcgg caggcatgcc
    10801 gaggcggctc aagtcggcgg tggcgagacg actggccgta gggaaggttc gggtgaggcg
    10861 tatctcgccc gaattgagta agcgatcacc ccacagatcg accagtatgc caccgaggcg
    10921 gcgtgcagcc tcgacggtga tctgctgtcc caacacagcc cggactgcca gttcaaaacc
    10981 gtcccagcag ccggggacgc gtaatccagg ccgtaaggcg atcaggggag cgagggctcc
    11041 gtccttggac aaatgagtcg caatgctgtc gacgtcagcg cccacgtcga actgatgccg
    11101 gacgcgagct actatcgctt gaagtgcgcg gacagatgga aagcggatcg tggccaggag
    11161 gcaatgccga gcaggattgt gcgcaacggt gacagtgccc tgcagcccat ccagtaggac
    11221 gctgcgccgg tacaggccat cttggaccac ttccagacca tcgatagcgc gggcacggag
    11281 atagtccaac atgttctgcc agtcatacgg ctgttgatag ggaagctgca gcaccacagg
    11341 agcatcggcg gggagagtcg aggatggctg cgcgctgcaa cggcgcaact ccccgggtgg
    11401 tgtgtggtaa agggtgtgga acacatggtt gaagcggcgt acgctgccaa agcctccggc
    11461 aatggccacg tcggtcattg acagcctcgt atcgtggagt aactgtttcg cgaacagcag
    11521 tcgcctggtt tgagcgacga ccaccggtgc cacaccaagg tgctggtcga agagccggcg
    11581 caattgccgt gcacccatcc cgacgtgcgt cgcaagtgcg tctacggtac cgtcctcacg
    11641 atcgaggaaa ccctcggcga tcagcgccaa cgcacgagag acggtgttcg atgtgccgcg
    11701 ccagccggcc agctcgggag cgatttcggg acggcagcgc aggcaggcac ggaatccggc
    11761 agcctgggcc gacgcactgt tggcgaaaaa ccgacagtgt tcacgtttgg gtgtccgggc
    11821 cggacagatc ggacgacaat agataccggt cgagacgacc ccgacgaaga gtcgtccatc
    11881 gaagcgcgga tcgcgggtct gcagggcgcg gtagctgata tcgggatcga gcggttccat
    11941 gcccatactc tgccacatcc gcctgagaaa ttccggccgt ttacggacgc gatcatgaac
    12001 cggcaatcaa cggcgtctgc cggtttcgtc atgacggccg agttccaata ttcctcgggg
    12061 tgattgtgac tcacgattgc gagcgagcct caactcagga cgaggataca tctcccatcg
    12121 accaacaaca ccgctgtctc gtgccgggcg atttccgcct gtgaccgatt gcgatccgac
    12181 ccattagcta gccgagcgta gagccgttcc acgcgtccat gtcaacggta caatgacgcg
    12241 cgcgtcgtaa taaactcatg gtcattcgtc tggggccgaa atactgtttg gttttcggtt
    12301 agaagaggac tatcgtcctt cccgttcgtc cctctccatt gtaagaccta ggcattttca
    12361 catctcccgc accgtaagtg aggctgcgca tgcctccggt gagcacgcgg tcgatcactt
    12421 cataaaaagg aggtccgcat gtctaccgtc cttcagccgc cgacacacag cgcccctgtt
    12481 cccaccgaca tcaccgactg gagcaacacg gcgatgaacc tcattgccag caccttgggc
    12541 ttgatccctg aagttggatc cctgctcagc ggtctggtct atatcctatg gccgagtgat
    12601 caagaagacg tctgggacga gatcaaggat caagtgcaag cgctcgtcca gcaggacctc
    12661 acacagttgg ttgagaatct ggaagtccaa acgttgcaag gtctcaaggg gtcgatccag
    12721 gattatcagg atgcgctcac caccaacgat ccatccaaca tttcgcagaa ttggaccagt
    12781 gcgaagctca attttgttca acagatgccg cagtttcaga cgcaggggta cgaggtagca
    12841 ttgcttccct attacgccca agcggtgaac ctgtatctcg cgctgctccg ggacggcgtg
    12901 ctgcatggta aagactgggg atggaccgat gccgatgtgg ccgtgaccac gaaggaaatg
    12961 aatcaggcca cccaggatgt ttgccaatgg gtggacacga cattcgggca ggagtatggg
    13021 cggcgcacaa gtgccttcgg ggcggcccaa gatgcctatc tgcaattctg caattccggc
    13081 aacgatccgg tgaattattc attaggctat cactacaatg tgacggcgtg gaacaagcag
    13141 aacaacttcc tcacgtggat gacgctgaag gcgctcgact ttcgaaacct gtggccattt
    13201 tttaattcag gccaggtcga tgtagtgctg catcgtgaga tctacagcat ggcctttggc
    13261 tcgtcggact actcgccgat accgattcgg ccctggtgga gtctcaatcc gccggccccg
    13321 acccaaccgc tcacgacgat cgacgtgttt gccgcagacc ggatcaatgg cgtgcgtctc
    13381 aactatccgt ccggtggcgg gccgggcggt gaaacgcagg ttgtgtgcgg cgcgctcaca
    13441 gacggttgtg tcacggtcta tgccgatgca gacccgattg ttgaagtgga tatctatgcg
    13501 ggtgatatcg tcagtgcgtt gagttttact ggccaggatg gctcatccac cggtcagatc
    13561 ggaggcaaca agccgccggc ccgcagcccc ataggtttta tttttcgtga gaattcgatc
    13621 ctctcaagtc tctatatcca cggcaccagc gagttttacc aaagcgccga tctgatcatt
    13681 gtgggatatc agtatcagca gaaggcaacc accgacctga cggcattgcg acatctgtac
    13741 gtgtgctcgc cgtcgcagat cgatctgacc gaattggcgg cgcgctgcgt gaccaagcct
    13801 gtctcgatcg atgcgctgaa ggccgcggcg gacacggaag ggtgggatgc gcagcgccaa
    13861 caataccgga acagcctgac gcgtcggtcg accgccgcca accgtccacg gagggcgtca
    13921 tgactaccgt cacgtctcca gaggccctct ccattcataa gttgttgcag attccggcca
    13981 gctttgcctg gaacttcgac cccggccacc ttgccacgat cggatgttcc ggagacattg
    14041 acggcgacgg gcgcgacgaa ttcatcgtca gctcaacgca agcaaccagt ccggtcgcct
    14101 gtctcagtgt cttgtcgtac gtgcagccga atgaactcga ccccggatgg agcggcaatc
    14161 agcagccgat gctgatagtg cagtggtcat cctccgccgg aattccgaat tggatgtatc
    14221 ctccatcgcc ggcgccacag tcctgctcca atttctcctt ttggtcggcg gatgtcgatg
    14281 gcgatgggat ccaggaaatc atcgccttct tccccggtta caccggagcg tctgccagcc
    14341 tcggtgtcct caagtgggac ggccacagct tgagctgcgt gtggcaaacg ttcggggtca
    14401 tttcgggcat cccagggact cattcgtgcc cgctggcgcc gggactccag ttgtatccca
    14461 tgcatagtcc agccggtgag ggtggagacc ggatcctgtt cgttcagccc gtgtcaggcc
    14521 tcggcacgct cgtcggcctg atggagtgga atgcaggagc attcagctct gtatggctga
    14581 ataacggttc gatcccgggg gctgggaagg ttcctgcgtg gacgttgggc cccgtcgatc
    14641 agttcgcggt cgcggatgtg gacggcgata cggaggatga actcattctc cttgttccaa
    14701 gctatgccga caaggtcaat cagctcaatc ctgctctggc ggtggcgaaa tggtccggcg
    14761 gagtactgtc cgtcatctgg cacgtctcct cgtccgcggg caacgctccc gactacgtga
    14821 cccagtcggc aagtctcctg gtggccgatc tggatggtac gggagttgcc aaggtcatca
    14881 ttcccatggc ggaatataaa ggg
//
LOCUS       DAORQN010000028        14505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_272_length_14505_cov_8.423806, whole genome shotgun sequence.
ACCESSION   DAORQN010000028 DAORQN010000000
VERSION     DAORQN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_14505_cov_8.423806"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK614_03315"
     CDS             complement(<1..643)
                     /locus_tag="PK614_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81267.1"
                     /translation="MRVLVIEDETKVGSFIKRALEEESYAVDLCEDGAQGLDMALSGS
                     YDLIMIDLMLPSLPGLEVLTRLRKEKIQTPVLILTAQSKVDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGTAESSGTLQIDDLLLNPATREVTRGGQRIELTVKEYALLEY
                     FMRHAGRVLTRPMISDHVWNQDFDTFTNVIDVYVNYLRNKIDRGRARKLIHTIR"
     gene            complement(712..>1133)
                     /locus_tag="PK614_03320"
     CDS             complement(712..>1133)
                     /locus_tag="PK614_03320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRB81268.1"
                     /translation="LEQTAATLLLTAVSGDMSGGIAWGLEGARTMGSLHYTRHHETEA
                     DIEGLRMMQAARLDPAAMIAFYGTMERGAQDHAGPPDFLSTHPDMGERLATLIALAGP
                     SPSDAQRLLPGEDWKDIRTLCRLQAGGRSASASPELS"
     assembly_gap    1134..1143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1144..1863)
                     /locus_tag="PK614_03325"
     CDS             complement(<1144..1863)
                     /locus_tag="PK614_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB81269.1"
                     /translation="MPTSRSAHYLDGRTATRHGVTLTVAPATLHIVMPDGSTKQWPYD
                     EIRQTQGTYQGEPVRLEFGPEPAEAIVISTAALLDDIHEAAPALARHFHNPARRNARI
                     GWTLCAAVGVVLMVVSLYRWGIPGIASAATPYIPVAWEESLGRQVVAHLAPETQQCRD
                     PERLRKLDHIVQTLAATHPASPYRITLSVVDAPTVNAFAAPGGRLVILRGLLEQTASP
                     EQLAGVLAHELQHVYQRHTTKA"
     gene            complement(1869..3137)
                     /locus_tag="PK614_03330"
     CDS             complement(1869..3137)
                     /locus_tag="PK614_03330"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HRB81270.1"
                     /translation="MTTTDAYTVTGDRTLSITCHRCLGRYRIRDARRLTDRSRSLCPT
                     CGARFAVVVAAPAANQRHVEEQVPSPVLLQLLPTNTPESTPSIQRGSFHGAGGTLLGM
                     QIVNVCLTIVTLGAYHFWAKAKIRRYLFSQTAFAGDRFAYHGTGKELYQGFLKAMVVF
                     GIPYFSLGAAHSFLALPQSVDFLLQAMAALVLFLYVPVAIVNARRYRCTRTSWRGIRF
                     SFRGRTVDFLKLYLKGWLFTLLPLGTYYPYFQTQRQAFLHSHTYFGNQRFQFTGHGSG
                     LMVPFAVTLFTTYAVLCLCGLALALQLTNAGLTLLLIPFVLGPVWVWLLGQKQKYYWD
                     HTTFGEARFSSSITWQKLFGLYLGNLAFLLLTLGWAWPWVTVRNARFFIGTLSLQGLT
                     DLDRVLQDTTETSVTGEGLSNLLDTGFDMD"
     gene            complement(3141..4454)
                     /locus_tag="PK614_03335"
     CDS             complement(3141..4454)
                     /locus_tag="PK614_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRB81271.1"
                     /translation="MATVHPGTATSASAIASPLDSTQAVVCPFCQTLTPCAPVAGVPL
                     HQCPCPSCNENLLILNPDGQPADEDMVAPSSAGGSDATDMSRRLVGLAVALLACVLNY
                     ALLAGMASWMFNQIKSTHDPYDIDELTLSPLILKANGWTPLHLAAARGDLSEVTSLLE
                     DGSPVDRHNNNGRTALFEAATRGQTPVVTVLLQYGANPNARMKQNMTALLTAAEHGHA
                     DTIAVLLSNGADLNALCTCGDSALHRAVRKGHLAAAQILLEQGIAANQKSHGETALEI
                     AQHDEDRELIELLRAHGGREFSQAKAHRAQGVAFQKKGQVDKALFAYAEALNLDPDDP
                     EAYFDRGTALLEKDSPDEALIAFQAAIRLNSTFIEAYSAAASVHIARRQWDQALALWD
                     RFLALRPQDGRAHFERAIIKRAKGDSKGFLQELQQACTFGHRAAC"
     gene            complement(4617..6272)
                     /locus_tag="PK614_03340"
     CDS             complement(4617..6272)
                     /locus_tag="PK614_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRB81272.1"
                     /translation="MITHDILIVGAGLAGMRAAIAAPPDLNVALLSKVHPVRSHSVAA
                     QGGINAAIGEQDSWEAHAYDTAKGGLYLGDQDAIEAMCREAPQDILELERMGVIFSRT
                     PEGRIAQRPFGGAGFPRTCYAADRTGHALLHAMYEQLMKRRCRVYEEWYVTALLVEQG
                     RCCGVVAWDLVHGGLQVLRAKAVILATGGSGRVFSTSTNAVINTGDGMALAYRAGLPL
                     EDMEFVQFHPTTLKDTGILITEGARGEGGYLLNTLGERFMKRYAPEQMELATRSTVSL
                     AIGQEIQEGRGVDGCVLLDLRHLGRARILERLPQIRELALEFAGLDPIETPIPIRPGA
                     HYQMGGVKTNAWGETDLPGLFAAGECACVSVHGANRLGGNSLLETIVFGRRAGTRAAE
                     AIRDCDLQPVPEEVLKGEEQRLKTLLINPGPERAWQIRDDLGKTMSLNLGIFRTKQSM
                     QEALGAIQALKVRAQRMCVQDKGQIFNTDLIQALELQCLLDIADTIVVSALGREESRG
                     AHYRADFPTRNDSAWLCHTVSHHQTDGPRLSYVPVTITRFPPA"
     gene            6477..9362
                     /gene="gcvP"
                     /locus_tag="PK614_03345"
     CDS             6477..9362
                     /gene="gcvP"
                     /locus_tag="PK614_03345"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRB81273.1"
                     /translation="MPQPNFLEPNDTFVPRHIGPTDSDIQEMLATLSLPSLEALVEVT
                     VPSDIRLQTSLTVPSPRGEQQVLAELRGLAGQNQVWRSLIGMGYYDCVTPLVIQRNIL
                     EDPGWYTQYTPYQAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMTMC
                     AAMSRAAGHERKKFFVSENCHPQTIAVVQTRAEPLGIILQIGAIQSLDLSRDEFFGLL
                     LQYPSTDGYVGDYSEFITRAHASGAYVVVATDLLALTLLRSPGEFGADVAVGSSQRFG
                     VPLGFGGPHAAFLATKEEFRRQMPGRIIGVSKDVTGRVAYRLSLQTREQHIRREKATS
                     NICTAQVLLAVMAGMYAVYHGPAGLRRIAERIHGLTMVLAEGLRRHGCAVGLEPVFDT
                     LRVPLSPAQSDTILNRARQQKINLRQYDDQSLGLSLDEWSTVEEVQQVLALFVGHEIP
                     AEEFQAILASVDVRYPAPLARTSPYLTHPVFHRYHAEHELLRYIHRLQSRDLSLVHSM
                     IPLGSCTMKLNATAEMLPVTWPEFGRLHPFAPSEQAQGYQTLFQQLEAWLAELTGFAA
                     ISLQPNAGSQGEYAGLMVIRAHHRHRGETQRDVCLIPVSAHGTNPASASMCGMTVVPV
                     ACDEHGNVDLNDLEAKATQHRNRLAALMITYPSTHGVFEEGMRRMCQRVHTHGGQVYM
                     DGANMNAQVGLCRPADLGADVCHLNLHKTFCIPHGGGGPGMGPIGVARHLVPFLPGHP
                     VTKLGGPESIGPIAAAPYGSPSILTISWVYIALMGREGLTKATQVAILNANYMAKRLE
                     KYYPVLYSGTRGFVAHEFILDLRPLKESSGVEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESEVKAELDRLCEALIAIRGEIQSIAEGRQPRAGNVLKNAPHTALSVTAAEWT
                     KPYSREQAAFPAPWVRDNKFWPSVGRIDEAYGDRHLFCTCPPMDPAS"
     gene            9370..10008
                     /locus_tag="PK614_03350"
     CDS             9370..10008
                     /locus_tag="PK614_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81274.1"
                     /translation="MGGASSTDTATYRLPQVHAVLSIAAGCLLLPLFFSPPAWADLES
                     GAAAEMQQSSPMLDVDPGVTYRAGARLRIPGTDWSFLVPTGWQSTRPEDSEMPFLMAE
                     EGKGLGMMFPLTDVTRETVRDHLSQPLSLLHGLSFIPAGSEVETELSIARSYQGEDLA
                     GRALAVLGPGNTSVIYFLMGPPDEASGYQSVLEGLGQSTRFAAPVSGRDVGL"
     gene            10041..11027
                     /locus_tag="PK614_03355"
     CDS             10041..11027
                     /locus_tag="PK614_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRB81275.1"
                     /translation="MQRPSPTKVILDTDPGVDDALALLLAFASPELQVVGVTTVCGNV
                     PVGQATKNLFRLLNVVQPPPGLLVGQGAARPLEEDLVTAVQVHGSDGLGELDSVLAEE
                     GTPRYPQVRLPSVLSTAQDVWNECVRRYPDDVTLITLGPLTNVAVALKVNPLTVQKFR
                     SVIVMGGAIGVPGNVAPAAEFNMYVDPHAAHRVFQASLPLTLVPLDVTTRAGVTRESL
                     MTWAAESHAPLARMTTDMTRKAFEFAEKVEGHGLFYFHDPLAVLAAVDSSLLKVEPLH
                     VSVEMAGRVSRGITIADRRQRTPEEKARPNMRVAVDVDVDRTLSLLRNRLCP"
     gene            11024..11950
                     /gene="rbsK"
                     /locus_tag="PK614_03360"
     CDS             11024..11950
                     /gene="rbsK"
                     /locus_tag="PK614_03360"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRB81276.1"
                     /translation="MILVVGSSNIDLVASVDRLPSRGETVLGYRFAQSFGGKGANQAV
                     AAARAGANVAFLSKLGADANGRLIEQHLAAQGLSRPILLRDAEFPTGVAMILVDHSGE
                     NQIAVVPGSNGRLTPADLRQHRELIAGARVLLLQMEIPRETVFEALRLGRECGLTTIL
                     NPAPAAPLPSDLLRLVDILTPNESEAQALTGSADPAEAARILTDRGVGTVVVTCGANG
                     AFLTTGNDVTHIPGFLVETIDSTGAGDAFNGALACAVAEGVPIKSAIVRANAAGALAT
                     TGRGAQESMPTKDDIEQLCRSGVRQMEPRQRS"
     gene            complement(11993..12634)
                     /locus_tag="PK614_03365"
     CDS             complement(11993..12634)
                     /locus_tag="PK614_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81277.1"
                     /translation="MKCFPITMSLAIALLFVAEPAAAGTSVQQLTADALHECALGRQA
                     QTRAERVQHFAQGQAFGEQAVEKEDSSPDAHFSLFCNLGEQLRIDGESLTSLFGFRRM
                     MKELNRTLELAPDHLDALSAKGTVLVRIPAFMGGDREKGEMLLRQVIAREPAAVNARL
                     SLAKSYCANGRHEEAVAIATTALDLAQSLQRADFIPEARQVLSQLRAQAAKGN"
     gene            complement(12734..13279)
                     /locus_tag="PK614_03370"
     CDS             complement(12734..13279)
                     /locus_tag="PK614_03370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HRB81278.1"
                     /translation="MPKQPSLPDHLRPELSIVFVGINPGLRSAALGHHYAGPSNRFWK
                     LIYDANLVPEPLSYRDDGRLPEWGLGLTNLVSRSTASMESLTPQDYTAGRLVLMRKLL
                     RYRPRVVALLGMTLYPILFPLEPKQTGRKPGLQPATLYDSNIVLLPNPSGRNASYPYH
                     SMLEAFRTLTPWAKSGLKSPP"
     gene            13519..13743
                     /locus_tag="PK614_03375"
     CDS             13519..13743
                     /locus_tag="PK614_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81279.1"
                     /translation="MKKMMLTVMAAALLVAFSAPSFAGDDKKKEEKKGGHFSQTLFGE
                     EKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            complement(13820..14338)
                     /locus_tag="PK614_03380"
     CDS             complement(13820..14338)
                     /locus_tag="PK614_03380"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRB81280.1"
                     /translation="MVKPIYKGTVVTLNVDTVRLPNGHTIDLEVIRHPGASAVVPLKE
                     DGTVVLIRQFRHAANGFIYEIPAGKLHPKEDPLDCAARELEEEIGYKAGRFELLSSIF
                     TAPGFADEVIHIYLATGLTIGTQNLDQDEVLEVVEMPLREAIAKIEDGTIRDGKTIVG
                     LQAVYIRQERYR"
BASE COUNT         2724 a         4056 c         4676 g         3039 t
ORIGIN      
        1 ctcgaatcgt atggatcaat tttcgcgcac gtccgcgatc gatcttattc cgcaagtagt
       61 tcacatagac gtcgatcacg ttggtgaacg tgtcgaagtc ctgattccag acatggtcgg
      121 aaatcatggg acgagtcagc acccgtccgg catggcgcat gaaatattcc agcagcgcat
      181 attccttcac cgtcagctcg atgcgttgtc cgccgcgtgt gacttcgcgt gtggccggat
      241 tgagcaggag atcgtcgatt tggagcgtgc cggaggattc ggccgtgccg cgtcgtaaca
      301 gggcccgcac acgcgcgagc aattcatcga tggcgaaggg cttcgtgagg tagtcgtccg
      361 cgccggcgtc cagtcctttg acccgctggt ctactttcga ttgcgccgtc aggatcagca
      421 cgggagtctg aatcttttcc ttgcggaggc gtgtgagcac ttccagccca ggcaggctcg
      481 gcagcatgag atcgatcatg atgaggtcgt aactgccgct caaggccatg tcgaggcctt
      541 gcgcgccgtc ctcgcagagg tcgacggcgt aactctcctc ctcaagcgct cgcttgatga
      601 aggagcccac cttggtttca tcttcaatga cgaggacgcg catacatggg gcccataagt
      661 cggtggatta taccagaggc cgtcggatct gtgagcagcg gaatcgcggg gctaagacaa
      721 ctcgggggag gctgaggcgg aacgcccgcc tgcctgtagg cggcagagtg tacggatatc
      781 tttccagtct tctccgggga ggagccgttg tgcgtcggat ggggacgggc cggccagcgc
      841 gataagtgtg gcgagccgct cccccatgtc cggatgggtg gagagaaaat caggcggccc
      901 ggcatggtcc tgcgcccctc tctccatggt tccgtagaag gcgatcatgg cggcgggatc
      961 gaggcgggcg gcctgcatca tacgaagtcc ttcgatatcg gcctccgttt catggtggcg
     1021 ggtgtagtgc aatgagccca tcgtgcgcgc gccttcgagg ccccaggcaa tcccgccgga
     1081 catgtcgccg gagacggcgg tcagcagcaa tgtggcggct gtctgttcaa gaannnnnnn
     1141 nnnggctttg gtcgtatggc gttgatagac atgttggagt tcgtgggcga gcactccggc
     1201 cagttgctct gggctggccg tctgttccag cagcccgcgg agaatcacca gccggccgcc
     1261 gggcgcggcg aatgcgttga cggtcggggc atcgacgacg gaaagggtga tccgataggg
     1321 agacgcagga tgagtggcgg ccagagtttg cacgatgtgg tcgagtttgc gcaatcgctc
     1381 cggatcgcga cactgctgtg tttccggggc aaggtgcgcg acgacctgcc ggcccaggga
     1441 ttcttcccac gccacgggta tatagggcgt cgcggcggag gcgataccgg ggatgcccca
     1501 tcgatagagg ctcacgacca tgaggacgac gccgactgcg gcacagaggg tccaaccgat
     1561 gcgcgcgttt cgtctggccg ggttgtggaa gtgccgtgcg agagccggcg ccgcctcatg
     1621 aatgtcatcc agcaacgcgg ctgtggaaat cacgatggct tcggccggtt caggcccgaa
     1681 ttccaggcgg accggttcgc cctggtaagt cccttgcgtc tggcggatct cgtcataggg
     1741 ccattgtttg gtgctgccgt ctggcatcac gatgtgtagg gtcgcggggg cgaccgtgag
     1801 ggtgacgcca tggcgcgtgg ccgtgcgacc gtcgagatag tgggcgctcc ggctggtggg
     1861 catgcgggct agtccatatc gaagccggtg tcgagcaggt tcgacaggcc ttcgccggtg
     1921 acggatgttt cggtggtgtc ttgcaggacg cggtcgagat ccgtgagacc ctgcaacgaa
     1981 agcgtgccga tgaagaagcg ggcgttccgg acggtcaccc atggccaggc ccaccccaac
     2041 gtgagcagca agaaggcaag gttgccgaga tagaggccga acagtttttg ccaggtgatg
     2101 ctggatgaaa atcgtgcctc gccgaacgtg gtgtgatccc agtagtactt ctgtttctgc
     2161 ccgagcagcc acacccacac cggaccgagc acgaagggaa tcaggagcag cgtgagcccg
     2221 gcgttcgtca actggagggc cagggcgagg ccgcacaggc acagtaccgc gtaggtggtg
     2281 aagagtgtga cggcaaaggg caccatcagg cccgatccgt gtccggtaaa ctggaaccgc
     2341 tgattgccga aataggtgtg ggagtggaga aatgcctgcc gctgcgtctg gaaataggga
     2401 tagtaggtgc ccaggggcag gagggtgaag agccaccctt tgagatagag cttgaggaag
     2461 tccaccgttc gtcctcgaaa cgagaatcgg atgcctcgcc aggaggtgcg cgtgcagcga
     2521 taccgccgcg cattgacgat ggcgaccggg acatagagga acagcaccag cgcagccatc
     2581 gcttgtaata ggaagtcgac cgattgcggg agggccagaa agctatgcgc ggcgcctaac
     2641 gaaaaatagg gaatgccgaa gaccaccatc gctttcagga acccctggta aagttctttt
     2701 ccggtgccgt gataggcaaa ccggtctccc gcgaatgcgg tttgactgaa caggtaccgg
     2761 cggatcttcg ctttggccca aaagtggtag gcgccaaggg tgacgatggt caggcagacg
     2821 ttgacgatct gcatgcccag caaggtgccg ccggctccgt gaaaggaccc gcgttgtatt
     2881 gagggggtgg attcaggggt gttggttggc agaagctgga ggagcaccgg cgaagggacc
     2941 tgctcctcaa cgtgtcgttg atttgcagca ggtgcggcga ctacgactgc gaatcgtgca
     3001 ccgcaagtcg ggcagaggct tcgggatctg tccgtcaatc ggcgtgcgtc gcgaatccga
     3061 tagcggccga gacagcggtg acaggttatg ctgagagtgc ggtcgccggt cacggtatac
     3121 gcgtcggtgg tggtcatggt tcagcaggcc gcccgatgcc cgaaggtgca ggcctgttgc
     3181 agttcctgca ggaaaccctt gctgtctccc ttcgctcgtt tgatgatcgc tcgttcaaaa
     3241 tgggcgcggc cgtcctgtgg ccgtagtgcc aggaatcggt cccacaaggc taaggcctgg
     3301 tcccactgtc ggcgcgcgat gtgcaccgag gccgcggcgc tataggcctc gatgaaggtt
     3361 gaattgaggc ggatcgcggc ttgaaaggcg ataagtgctt catcggggga gtccttttcg
     3421 agcagcgcgg tgccgcggtc gaaataggct tccgggtcat ccgggtccag gttcagggct
     3481 tcggcgtatg cgaaaagtgc cttatcgacc tgtcccttct tctggaaggc tacgccttgg
     3541 gcacgatggg ccttggcctg ggagaattct ctgcctccgt gcgcgcgcag cagctcgatc
     3601 aattcccgat cctcgtcatg ctgcgcgatc tcaagggcgg tctctccatg gctcttttgg
     3661 tttgcggcga tgccctgctc cagcaggatt tgagcggcag ccagatgccc tttccgtacg
     3721 gcccggtgta gcgctgaatc gccacaggtg cacagcgcat tgagatcggc gccgttggac
     3781 aggagcacgg caatggtgtc ggcatgtccg tgctcagccg ccgtcagcag cgcggtcatg
     3841 ttttgtttca ttctcgcgtt ggggttggct ccatattgca gcagcaccgt caccaccggt
     3901 gtctgcccgc gcgttgctgc ttcgaacaag gccgttcgtc cattgttatt gtgtcggtcg
     3961 accgggctcc cgtcctccag cagcgatgtc acctcggaca gatctcctcg tgccgctgcg
     4021 agatgaagcg gtgtccaacc gttggctttc aagatgagcg gggagagcgt caattcatcg
     4081 atgtcatagg gatcatgcgt cgatttgatc tgattgaaca tccaggacgc catgccggcc
     4141 agcagtgcat agttcaagac acaggccagc agagccacag cgagacctac gagacgccgt
     4201 gacatgtcgg tggcatcaga cccgccggcc gacgatgggg cgaccatgtc ctcatcggct
     4261 ggttgcccgt ccgggttgag aatcagcaga ttttcgttgc aggagggaca gggacattga
     4321 tgtaggggga caccggcaac aggcgcgcaa ggggtgaggg tttggcaaaa ggggcagacg
     4381 accgcctgcg tcgaatccag cggcgaagcg atggcggacg cagatgttgc tgtgcctggg
     4441 tgaacggttg ccatggtgcc taacgaccct tcgcccaggc ttgttgtgga cggtcccgtg
     4501 agtggagcga tcggtgatta ttgaaaagga tagcgcaaaa cggtaggagc gcaaccggcc
     4561 ggccggttta gcggctactc atcgagcagg ggacgtctgg gacgggaatc gaggcgttat
     4621 gcgggaggga atcgtgtaat ggtgacggga acatagctca accggggccc gtccgtctga
     4681 tggtggctta cggtgtggca gagccaggcg ctgtcgtttc gagtggggaa atcggcccga
     4741 tagtgggccc cgcgactttc ttcccgtccg agggcgctca cgacgatggt gtccgcgatg
     4801 tcgagtaggc actggagttc aagcgcctgg atcaggtcgg tgttgaagat ctgtcccttg
     4861 tcctgaacgc acatccgttg tgcacggacc ttgagggctt gaatggcccc cagggcctct
     4921 tgcatggact gcttggtgcg gaagatgccc agattgaggc tcatggtctt gccgaggtca
     4981 tcccgtatct gccaggcccg ctcggggccg ggattaatca gcagggtctt caatcgctgt
     5041 tcttcaccct tgaggacttc ttcgggaact ggctgcaggt cacagtcgcg gatcgcctcg
     5101 gcggctctcg tgccggcccg gcgtccgaag acaatggttt cgaggaggga attccctccc
     5161 aggcgattgg ctccgtgcac actcacgcaa gcgcattcgc ctgcggcgaa gagtcccggt
     5221 aggtcggtct ctccccaagc gttggttttc acgccgccca tttggtaatg ggcgccgggt
     5281 cgaatgggaa tgggtgtttc gatcggatcg agtccggcga attccagggc cagctctcga
     5341 atctgcggga gccgctccag aatacgggcg cggccgaggt gtcgaaggtc gagtaagacg
     5401 cagccgtcga cgcctcgtcc ttcttgaatt tcctggccga tggccagcga caccgtggag
     5461 cgggtcgcca gctccatctg ctccggcgca tatcgtttca tgaaccgctc gccgagcgtg
     5521 ttcagcaaat agccgccttc cccgcgcgcg ccctcggtga tcagaatgcc agtgtctttc
     5581 aaggtggtcg gatggaactg gacgaactcc atgtcttcca gcgggaggcc cgcgcgatag
     5641 gccagggcca tgccgtctcc ggtgttaatg acggcattcg tgctggtcga aaagacgcgg
     5701 ccgctcccgc cggtggcaag aatgacggcc tttgcacgca agacctgcaa tcctccatgg
     5761 accagatccc aggccaccac tccgcaacaa cggccctgtt cgaccagcaa cgccgtgacg
     5821 taccattctt cgtacacccg gcaccgtcgc ttcatcagct gctcgtacat ggcgtgcaac
     5881 agcgcgtgcc cggtgcggtc ggcggcatag caggttcgtg gaaatccggc tcccccgaac
     5941 ggccgttggg cgattcgccc ctccggggtg cggctgaaga tcacacccat gcgttcaagc
     6001 tctaaaatgt cttgcggggc ttcccggcac atggcctcga tcgcatcctg atcgcccagg
     6061 taaagtcccc ccttggccgt gtcataggca tgggcttccc aggagtcctg ctccccgatg
     6121 gccgcgttaa ttccgccttg ggcggccacg gagtggctgc ggaccggatg aaccttggag
     6181 aggagtgcca cattgaggtc aggcggtgcg gcaatagccg cccgcattcc ggcgagtccc
     6241 gctccgacga tcaagatgtc atgtgtgatc atggtcttcg tatcctggcc accgttcagc
     6301 cgtgcgatcg acatcccggg agggacatcc gatgtcacta ttacgctatg gaatttcgga
     6361 aaacaagcga cgggcgatgg gcgctgaacg cttggaaaac tcggtcctgc atgataggtt
     6421 agcccacgcc gcttcgaatg cacgagagcg tcaactttta gcaggatttc acccccatgc
     6481 cacaaccgaa tttccttgaa ccgaacgata cgtttgtgcc ccgccatatt ggcccgacgg
     6541 attccgacat ccaggagatg ttggccacat tgagcctgcc gtcgttggag gccctcgtcg
     6601 aggtgacggt cccgtccgat attcgcttgc agacctcctt gacggtgccg tccccgcggg
     6661 gtgagcagca ggtcctggcg gaattgcgtg gcttggccgg acagaatcag gtgtggcggt
     6721 ctctgatcgg catggggtac tacgattgcg tcacgccgtt ggtgatccag cggaacattc
     6781 tggaggatcc cggatggtat acgcagtaca ccccgtatca agccgagatt gcgcaggggc
     6841 gtcttgaggc actggtcaat tttcagacca tggtcgccga tctgaccggt ttaccgctcg
     6901 ccaatgcgtc gctcctcgac gaagccactg ctgcggccga ggcgatgacg atgtgtgcgg
     6961 ccatgtctcg agcggccggg catgagcgca agaagttctt cgtctctgag aattgccatc
     7021 cgcaaacgat tgcggtggtg cagacccgtg cggaaccgct gggcatcatc ctgcagatcg
     7081 gtgcgattca gtcgttggat ctttcccggg acgagttctt cgggctgttg cttcaatacc
     7141 cctcgacgga cgggtacgtc ggtgactaca gcgagttcat cacgcgcgcg catgcctcgg
     7201 gggcctacgt cgtggtggcg accgacttgc tggcgctcac gctgctgcgt tcaccgggag
     7261 aattcggagc ggacgtggcg gtcggatcca gccaacggtt cggtgtcccg cttggattcg
     7321 gtgggcccca tgcggcgttt cttgccacca aggaggagtt tcgccggcag atgccgggcc
     7381 ggatcatcgg cgtctcgaaa gatgtgacgg gccgcgtggc ctatcgcttg tcgctgcaga
     7441 cccgggaaca acatatccgg cgcgagaagg ccaccagcaa tatctgcacg gcgcaagtgc
     7501 tgttggccgt catggcgggg atgtatgcgg tctatcacgg tcctgcaggg ttgcggcgga
     7561 ttgcagagcg aattcacggc ctgacgatgg tactggctga aggtttgcgc cggcatggtt
     7621 gtgccgtcgg cctggagccg gtgttcgaca cgttgcgtgt tccactgtcg ccggcgcaat
     7681 cggacaccat tctgaaccgg gcgcgacagc agaagatcaa tctccgtcag tacgacgatc
     7741 agagcctggg gctttcgttg gatgaatgga gtacggttga ggaagtccag caggtgttgg
     7801 cgctcttcgt cggtcatgag atccctgccg aagaatttca ggccattctc gcctcggtcg
     7861 atgtgcgtta tcccgcaccg ctcgcgcgca cgagccccta tctcacgcat ccggtgtttc
     7921 accggtacca tgccgaacat gaacttttac gctacatcca ccggcttcag tcccgcgact
     7981 tgtcgctcgt gcattccatg attcccttgg gatcctgcac gatgaaactg aatgcgacgg
     8041 cggaaatgtt gccggtgacc tggccggagt tcgggcgcct ccacccgttt gctccgtccg
     8101 aacaggcgca gggataccag acgctgttcc aacagctcga agcctggttg gctgaactga
     8161 ccgggtttgc cgccatttcg ctgcaaccca atgccggttc gcagggggag tatgcagggt
     8221 tgatggtgat ccgtgcgcat catcggcatc gcggcgaaac gcaacgcgac gtttgcttga
     8281 ttccagtgtc ggcgcacggg accaatcctg cgagcgcgtc gatgtgcgga atgacggtgg
     8341 tcccggtggc ctgtgacgag catggcaatg tggacctgaa cgatcttgag gccaaagcca
     8401 ctcagcatcg caaccgcctc gcggcgttga tgatcaccta tccctcgacc cacggtgttt
     8461 ttgaggaagg gatgcgccgc atgtgccagc gcgtgcacac gcatggcggg caggtttata
     8521 tggacggggc caacatgaat gctcaggtgg ggctctgtcg gccggccgac ctgggggcgg
     8581 acgtctgtca cctgaatctt cacaagacgt tctgcatccc tcacggcggt ggcgggcccg
     8641 ggatgggacc gatcggggtc gcccgtcatt tggtgccgtt tctgcccgga cacccagtca
     8701 ccaaactggg tggaccggag tcgatcggcc cgatcgccgc cgcgccgtac ggcagcccga
     8761 gcatcctcac gatttcctgg gtctatattg ccctgatggg acgagagggc ctgaccaaag
     8821 ccacgcaggt ggccattctg aacgccaact atatggccaa acgattagaa aagtattacc
     8881 ccgtgttgta tagcggcacc cgtggcttcg tcgcacatga gttcattttg gatctgcgcc
     8941 cgctcaagga gagcagcggt gtggaagcga tggacgtagc caagcgtctc atggattacg
     9001 ggttccacgc tcccacggtc tcgtttcccg tggccggcac cttgatgatc gagccgacgg
     9061 agagcgaagt gaaggcggag ttggatcggt tgtgcgaagc cttgatcgcg atccggggcg
     9121 agattcaatc gattgccgaa ggccggcaac cgcgtgcggg caatgttcta aaaaatgctc
     9181 ctcacacggc attatctgtg accgcggctg agtggacgaa gccgtactcg cgtgagcagg
     9241 cggcgtttcc tgctccgtgg gtgcgtgaca acaaattttg gccgagcgtg ggtcgtatcg
     9301 atgaagccta tggcgaccgc cacctctttt gcacctgtcc tccgatggat cccgcttcct
     9361 aatcgatctg tgggcggtgc ctcctcgacg gacacggcga catatcgcct gccgcaggtg
     9421 catgccgttc tttcgatcgc cgccggttgt cttctgttgc ccctgttctt ctcgccgccg
     9481 gcttgggctg atttagaatc gggcgcggct gcggagatgc aacagagcag tcccatgctc
     9541 gatgtcgatc ccggcgtgac gtaccgcgcc ggcgccaggc tcagaattcc cgggaccgat
     9601 tggtcgttcc tggtgccgac cggttggcaa agtactcgcc ccgaagactc ggagatgccc
     9661 tttctgatgg cggaagaagg gaagggcctg ggaatgatgt ttcccctcac cgatgtgacc
     9721 cgggagacgg tccgcgatca cctcagtcaa cccctgtcac tgttacacgg gttgtcgttt
     9781 attcctgccg gctctgaagt tgagactgaa ctctcgatcg cccgctcgta tcagggtgaa
     9841 gacctggctg gtcgtgcctt ggcagtcctc gggcccggca atacgtcggt gatatatttt
     9901 cttatgggac ctcccgacga ggcgtcaggg tatcagtccg ttctggaagg acttggacaa
     9961 tctacccgat ttgccgcacc ggtatccggg cgtgacgtcg gcttgtaata ggggtcgatt
    10021 ccctatcaaa tcttctaggc atgcaaagac cctctcccac gaaagtgatt ctcgataccg
    10081 atccaggagt cgatgacgcc ctggcgctgt tgctggcgtt cgcctcgcca gaattgcagg
    10141 tcgtcggggt gaccaccgtg tgcgggaacg tgccggtggg acaggcgacg aagaatttgt
    10201 tccgcctgct gaacgttgta cagccacccc cggggctgtt ggtcggacag ggcgcggcgc
    10261 ggcctttgga ggaggatctt gtgaccgcgg tccaggtcca cgggagcgac gggcttggcg
    10321 aactcgactc tgtcctggcc gaggagggga cgccgcgata ccctcaagtc cggttgcctt
    10381 ctgtgctctc aactgcccag gatgtttgga atgaatgtgt ccgccggtat cccgatgatg
    10441 tcacgctgat cacgttagga ccactgacga atgtggccgt ggcgttgaag gtgaatcccc
    10501 tgacggttca gaagttccgt tcggtgatcg tgatgggggg cgcgatcggc gtacccggca
    10561 atgttgctcc cgcagcagag tttaatatgt atgtggatcc gcatgcggcg catcgggtgt
    10621 ttcaggcgtc gctcccgctc acgctcgttc cgctcgatgt gacaacccga gccggagtga
    10681 cacgggagag cttgatgacc tgggccgctg agtcgcatgc cccgctcgct cggatgacaa
    10741 ctgacatgac gcggaaggca ttcgagttcg ctgaaaaggt tgaagggcat gggctcttct
    10801 atttccacga tccgttggcc gttctggccg ccgtcgattc ctccttgttg aaggtagagc
    10861 cattgcatgt gtcggttgaa atggccgggc gagtgtctcg cgggattacc atcgccgatc
    10921 gtcgtcagcg cacgccggag gagaaggcac gtccgaacat gcgcgtggcc gttgatgtcg
    10981 atgtcgaccg gacgctgagt ctcctacgca accggctgtg cccatgatcc tagtggtcgg
    11041 gtccagcaat atcgatttgg tggcgtcggt cgatcgcctg ccgagtcgag gagaaaccgt
    11101 cctcgggtac cgtttcgccc aatcattcgg cggcaagggc gcaaatcagg ccgtcgcggc
    11161 agcccgcgcc ggtgcgaatg tcgcatttct cagcaagctg ggggcggatg ccaatggacg
    11221 gttgatcgaa cagcacttgg ccgcgcaggg gctgtcgcgc ccgatccttc ttcgcgatgc
    11281 cgaattcccc accggagttg cgatgattct cgtcgatcac tccggagaga atcagattgc
    11341 ggtcgtgccg gggagcaatg ggcgtctgac accagctgat ctgcggcagc atcgtgagtt
    11401 gatagccgga gcacgggtgt tgttgcttca aatggaaatt ccgcgggaga ctgttttcga
    11461 agcgttgagg cttggccgag aatgcggtct caccacgatt cttaatccgg ctccggctgc
    11521 tccattgccg tcggacctcc tccgattggt cgatatcctc actcccaacg aaagcgaagc
    11581 gcaggccttg accggttcag cggatccggc agaggcggca cggattctca ctgatcgtgg
    11641 cgtcggcacc gtggtcgtga cctgtggcgc aaacggggcg ttcctgacaa ctgggaacga
    11701 cgtgacccat atccccggct ttcttgtcga gacgattgat tcgacaggcg ccggagatgc
    11761 gtttaacggt gctttggcct gtgctgtggc tgaaggggta ccgatcaaga gcgcgatcgt
    11821 gcgggctaat gcggccggtg cattggccac caccggtcgt ggggcgcagg agtcgatgcc
    11881 gaccaaagat gacatcgagc agttatgtcg atccggggtc aggcagatgg aaccgcggca
    11941 gcgttcgtaa gtcggtaggc ctgatttcag gagagtggca gaagcgggct gctcaattac
    12001 ctttggccgc ttgggctcgc aactgggaca gcacttgccg ggcttcggga atgaagtctg
    12061 cgcgctgaag gctttgcgcg agatccagcg ccgttgtcgc gatagcgacc gcttcttcat
    12121 gtcgcccatt ggcgcaatag ctctttgcca ggctcaatcg ggcattgacg gcagccggtt
    12181 ccctggcaat gacctgacgc agcagcatct cccccttttc tctatcccca cccatgaatg
    12241 ccggaatgcg caccaggacg gtccccttgg ctgagagggc atccaaatgg tccggagcca
    12301 gttcgagggt gcggttcaac tccttcatca tgcgccggaa gccgaagagc gaggtcaggc
    12361 tttccccgtc gatccgcagt tgctcaccga ggttacaaaa cagcgagaag tgggcatcgg
    12421 gcgatgagtc ctccttttct accgcctgtt ctccaaacgc ttgaccttgt gcgaaatgtt
    12481 gaacgcgttc cgcacgagtc tgagcctgac ggcccagcgc acattcgtgc agcgcatcgg
    12541 ccgtgagttg ttgaaccgag gtgcctgcgg cagccggctc ggcaacgaat aacagagcga
    12601 tcgcaaggct catcgtgatg ggaaaacatt tcatggagtg tccgtttcgg tgtgcccgaa
    12661 ggaaatcagc cgcagatttt acagcaggtc cccgacggaa ttccagcgga gcgcacggcg
    12721 tgaataggat gagtcaggga ggactcttga ggccgctctt ggcccacggt gtcagcgtac
    12781 gaaaagcctc cagcatggaa tggtagggat aggatgcgtt tcgcccactc gggtttggga
    12841 gtagcacgat attggagtcg tacagtgtcg ctggttgcag acctggtttt cgtcctgtct
    12901 gttttggttc gagtggaaag aggatggggt agagcgtcat gccgagcagg gcgacaacac
    12961 gcgggcgata ccggagaagt ttccgcatga gcacaagtcg gccggccgta taatcctgcg
    13021 gcgtgagact ctccatgctc gcggtcgatc ggctgaccag attggtgagt cccaatcccc
    13081 attccggtag tcgcccgtca tctcggtagc tgagtggttc cggcacaagg ttcgcgtcgt
    13141 aaatcagttt ccaaaaacga ttcgaaggtc ccgcgtagtg atgacccagc gcggctgagc
    13201 gtagcccagg attgatgccc acgaatacga tgctgagttc cgggcggaga tgatctggca
    13261 gagaaggctg ttttggcatg atggtcgtga gagcgtacct tgtcgcgtct gctgaatcga
    13321 ctgatgaggc ggtttagtat cgtccaggct attctgccac agaatcgagc tgtgctttct
    13381 gtagcattac gaagtgcgat gcatgacatg tccgaaaaaa caacagttta gatctgtggg
    13441 tgtatgaggc aaggactttg ctttaccgaa catccgtcgg tcgttggtaa tttcaaacct
    13501 aacaggaggt gagtcagaat gaagaagatg atgttgacgg tgatggcggc tgcattgttg
    13561 gtggcgttca gcgctccttc gttcgccggt gacgacaaga agaaggaaga gaagaagggc
    13621 ggccacttct cccagaccct cttcggtgaa gagaagaaga aggaagagaa gaagggtgga
    13681 cacctgtccc agaccgtgtt cggcgaagag aagaagaagg acgagaaaaa gggcggccac
    13741 taaacacaac accgtttctg gcgaggctag ctggcttggg aggtctccca tcctggaggt
    13801 gggagacctc ttgggtcgtc taccggtatc gctcctgccg aatatagact gcttgtagcc
    13861 ctacgatcgt ttttccgtcc cgaatggttc catcctcgat tttcgcaatg gcctcgcgga
    13921 gaggcatttc caccacttcc aggacttcat cctgatccag attttgcgtg ccgatcgtca
    13981 ggcccgtcgc aagatagatg tggatgactt catccgcaaa tcccggcgcg gtgaaaatgc
    14041 tggagaggag ttcgaagcga ccggccttgt agccgatctc ctcttcgagt tcgcgggctg
    14101 cacagtcgag tgggtcttct ttcggatgga gtttcccggc gggtatttcg taaataaaac
    14161 cgttcgccgc atgacggaat tggcggatca gcacgaccgt tccatcttct ttcaggggaa
    14221 cgacggcgga ggcgccggga tggcggatga cttcaagatc gatcgtatgg ccgttcggca
    14281 ggcgcaccgt atcgacgttg agcgtgacca ccgtgccctt gtagatcggt ttcaccatga
    14341 cttacggcgt gcccgcgggt ttcttgtaaa acggtggttt tacgacgacg gcggggcaag
    14401 ccctgcctcg aatatcgatc agaatggatg atcctggttg ggctgcagcg ggcgtgacat
    14461 atcccatacc gatacctttt tgtagcaagg gcgacaagtt tccgc
//
LOCUS       DAORQN010000029        14109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_276_length_14109_cov_473.173616, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000029 DAORQN010000000
VERSION     DAORQN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14109
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_276_length_14109_cov_473.173616"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PK614_03385"
     CDS             complement(<1..884)
                     /locus_tag="PK614_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB81281.1"
                     /translation="MAVLMYHRVLTKDEVARYPVQPGMYVLDSVFAQQMSFVRSNFTV
                     LSLQELLDLWQRGEWSAQARYCVITFDDGWLDNYRHAYPVLKQLRMPATIFLPTDYVG
                     RNEWFWPDQLAVLCKVVAARKQRQKPLAAVEGVLSGFLGEDASRLVEASVRQEQLGDE
                     IIERCKHLPIERIRELVQALAAELRVTLPLDRVIVNWDEVREMSRGGVSFGSHSCTHR
                     IMTTITLDEVSEELVRSRQVLFDQGVNFVPVYCYPNGNSDASIEDLVKANGYEAAVSV
                     RMGFEGSCPENRYAIRRVG"
     gene            complement(1006..1158)
                     /locus_tag="PK614_03390"
     CDS             complement(1006..1158)
                     /locus_tag="PK614_03390"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRB81282.1"
                     /translation="MGEFLTELWAFMKERKKFWLLPIIVMLVLLGSLIVLTQGSAVAP
                     FIYTLF"
     gene            complement(1175..1570)
                     /locus_tag="PK614_03395"
     CDS             complement(1175..1570)
                     /locus_tag="PK614_03395"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HRB81283.1"
                     /translation="MDTMVEKKELRNFGLIVGGVFILIGVWPMIRYGEGIRLWAIVPG
                     SLLVPLGLAAPTILAPLFRVWMKVGHVMGWINTRIILGILYFGLITPMGVIMRMFGWD
                     SMRRALSRDAESYRVVRQARPRNHMTRQF"
     gene            complement(1634..2773)
                     /locus_tag="PK614_03400"
     CDS             complement(1634..2773)
                     /locus_tag="PK614_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB81284.1"
                     /translation="MWFVEWIFWGSLIFIIYAYAGYLLALLVLSCFRNRPVLVGDIQP
                     KVSFVITAYNEEARIKEKIENSLQQQYPSERLEIVVASDCSSDRTDDIVRSYAALGVR
                     LVRAQERRGKEAAQKLAVSQTNGEILVFSDVATTLPPQGIANIVKSFSDPTVGCVSSV
                     DQFVDAQGNLSGEGAYVKYEMVLRHLETKVNTLVGLSGSFFAARRTVCSPWADDLQSD
                     FNTLLNSMKAGLRGVSDPASVGYYKNLTDEKKEYQRKVRTVLRGIAVLMRSLPMLNPL
                     RYGIFAWQLFSHKLCRWLVPFAMIGTLGSNIVLAISSGPYRVLLLGQILFYAIAIAYA
                     GCSWMPKSNVFRLPSFFVLVNLSIIDAWMRYFRGDRVFRWEPSKR"
     gene            complement(2834..4111)
                     /locus_tag="PK614_03405"
     CDS             complement(2834..4111)
                     /locus_tag="PK614_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81285.1"
                     /translation="MKAQRTLPPSAAPIEWRDLIQGLMGLVHPHETVGQLQAEFRQYF
                     GVKHVWFVSSGKAALSLIFRALHSLSGRSKVVLPGYTCFSVPSAVVRARLSVALCDVD
                     PLSLDFDFVQLSQVADSNVLCVLATHLLGIGVDVPRVVELCRQRGIFVVEDVAQAFGG
                     ERDGVPFGAMGDVSFLSFGRGKNITCGSGGAILTNDDRIGEVLAREYALLPEMSLADM
                     LKNWLEVAVTKVLLNPSLYWLPAGLPFLKLGETKFYTDFPITRLDPIRAGLLRRWKRR
                     LANSTTSRVAHSEQILRSLALSNVQTIKPSGRGQSVYLRLPVLMRSRQEKDAVCRTSA
                     EQGLGISPLYPSSLQHITKLRDTLSSQDVPQSTMIAERLVTLPTHELVSDSDIVRIST
                     IIQAAQRGDGAATIRRPDITNGQRHAPELPRNI"
     gene            complement(4108..5196)
                     /locus_tag="PK614_03410"
     CDS             complement(4108..5196)
                     /locus_tag="PK614_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HRB81286.1"
                     /translation="MWSLGQKNVRSVVVMSTMVSSILGRTNAEILAWESYVLSSPLAT
                     GYHQSGWRRVIEEAFGHPTHYLVVHGQDGALQGVLPLVLLASRGFGRFLVSLPFVNYG
                     GVVASSAEAHRFLEARAIEQARELNADHIELRHQALMDTAWVATERKVSMRLPLPSSY
                     EQLVKGFPSKLRSQVRRAQKEGMTARVGGSECLDEFYSVFTRCMRDLGTPVYAKGFFA
                     KILEVFPKESNICIVSHGQTPVAAGFLYGFRSSLEIPWAASDKRFNKLAPNMLLYGTV
                     LEYACQQGFQVFDFGRSSPDSGTYRFKEQWGAQPHQLYWYYWMKDGRPVPQLNPQNPK
                     YALAIRLWQKLPVVVANLIGPYIVKHLP"
     gene            complement(5165..6547)
                     /locus_tag="PK614_03415"
     CDS             complement(5165..6547)
                     /locus_tag="PK614_03415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81287.1"
                     /translation="MKRVLIIAYYFPPVAASGAMRPLGFCRNLPTFGWQPYVLTTAPE
                     CVYPAHRADEKLGERVPATVGVTRVPYTDRLQQILYYRDVLRRMVKRQVGQDRTGNLQ
                     TTQAPPSSPQSEAGRSTVKDFLLDWAFAFPDRQCGWLGQVVRHFQQFDEKQIPDVVFA
                     TGGPWTSFLVGSTLAKQFNRPLVLDYRDPWNCNPYYSFSSRLLTRKSQAIEARVCKAA
                     SHVIANTEELRQRLIEEYGELRGRCTWIPNGFDRDVLPLGQIPAEQLDSLSPSTGYEL
                     CHFGTVYGKRTPRVLLQAMWELFQEGRLKPEAVRLRFVGGWDSTDQECERYAAELEKR
                     SFLRREPPISHSLCLQEMQRSSVLLVLQPDSPLQVPAKIYEYVATGRPLLLIGGEGAT
                     ANLVTRHALGVSSPNQLAAIKVLLNELATGARKLAQPDVTRVNRFDYRSLTGDLAAVL
                     DVVIGAKECS"
     gene            complement(6715..8610)
                     /gene="asnB"
                     /locus_tag="PK614_03420"
     CDS             complement(6715..8610)
                     /gene="asnB"
                     /locus_tag="PK614_03420"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB81288.1"
                     /translation="MCGIAIAIGLNGRPIERVAVERMAKSLLHRGPDDGGIYLDGAVG
                     MGFRRLSILDLSEAGHQPMVSEDGQYVLVFNGEIFNYVELRSELRQLGYQFRSSGDSE
                     VLLAAYREWGRECLSKLNGMWAFVIYDRRHRRIFGSRDRFGVKPLYYSRSSDVMQFAS
                     EIKALRASGYQPGEINWRTAAKFLFEGRLESQVETFYQGIEQIPPGSGFELTLDGTWH
                     QWSFWSLDELPPSASKNPAATFADLFEDSVRIRMRSDVPVGVCLSGGLDSTAIICAAA
                     RQRGETAAGQSESLQAFCYMAKEFDESKYITDTLTQTRAQLRQLETSPAELWSDLRQL
                     LWFQDEPVHTMTAVVGYQLMRLAASNGIRVVLNGQGADETIGGYSSYFQDYWVSLLRQ
                     GRVREAWQAVTAYTEAHGGNSRQRLAAAAARCLSWEMYKFNAYRSWARARRQARLRQN
                     PWFSEDLTKHFINEDVSPASTTLSNSLKQSVVSAPLPLYLRIEDRNSMAHSVEARLPF
                     LDYRLVSFVSGLSDDWKIRGPWNKYVLREGMRGRIPESVRSRVDKMGFPTASKKWFAH
                     DLYEPLRDILGSQAVRERGIYNIGALTTDLERHHRGESDFANRLFHVAEFEIFADLLR
                     HDSRLAA"
     gene            complement(8663..9673)
                     /locus_tag="PK614_03425"
     CDS             complement(8663..9673)
                     /locus_tag="PK614_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB81289.1"
                     /translation="MRILITGGAGFLGSHLSDLLIGQGHDVIALDNLITGRAENISHL
                     IGNPKFSFVKYNVCDYLHVDGQLDAVMHFASPASPQDYLEMPIATLKVGALGTHKALG
                     LAKAKGARFLLASTSEVYGDPLLNPQPETYWGNVNPIGARGVYDEAKRFAEAMTMAYH
                     RYHGVDTRIVRIFNTYGPRMRPKDGRVVSNFIVQALQGKPLTVFGDGSQTRSFCYVDD
                     LVRGIVALLMVKSDKSVAERTDRTKFLTQKPDAILDSIHDPVNIGNPRELTVRGIAEI
                     ILKITGSKSVIEERPLPADDPKVRRPDITRAKSLLDWEPKVELEDGIRKATEYFRQVI
                     AK"
     gene            complement(9710..11554)
                     /locus_tag="PK614_03430"
     CDS             complement(9710..11554)
                     /locus_tag="PK614_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HRB81290.1"
                     /translation="MNILGISAFYHDSAACLVRDGEIIAAAQEERFTRKKHDPGFPHR
                     AIDYCLREGQIELKDVRHLVFYDKPLVKFERLLETYLAFAPRGVQSFVAAMPVWLKEK
                     LLLKSLLQKEFLQHAPDLTTSSLPQILFSEHHESHAASAFFPSPYEKAVVLCMDGVGE
                     WATTSAWLGQGNTLTPLWEIPFPHSIGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPK
                     YVKAIYEYLLDLKPDGTFRLNMDYFNYCTGLTMTGGKFDDVFGGPPRKPESKLTQREM
                     DLARSVQEVTEEVMLRLSRTLHRETGVDYMCMAGGVALNCVGNGRILREGPFKGLWIQ
                     PAAGDAGGALGAALTAWHQYEQQPRKVTSGKDGIKGSYLGPCHSNEEIEAYLKKVDAP
                     YVRLDENQLYARVAEELAAGKVVGWLQGRMEFGPRALGGRSILGDARNRDMQSVMNLK
                     IKYRESFRPFAPSVLRERVSDYFVLDADSPYMLLVAPVVEKRRLPVSSAQDGLWGIEL
                     LNIPRSDIPAVTHLDYSARIQTVHQDTNPRYYALLKAFEAKTGHAVLVNTSFNVRGEP
                     IVSTPEDAYRCFMRTEMDVLVLENCVLLKADQKPLEGDSDWKKEFELD"
     gene            complement(11670..12842)
                     /locus_tag="PK614_03435"
     CDS             complement(11670..12842)
                     /locus_tag="PK614_03435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81291.1"
                     /translation="MNIMLVTPWRPSLTGGISTVVARLTGEFQKKGHNITIFVADREN
                     RLRQIEALDEIPVYGMYLRSPVSSQYPLRAMVMCCIWFPITMVQLIWLARKKRLDAVL
                     IQYPLPAMFYFGILKRVFGGTLFITYQGNDAHDLCLWDRREQRLVRFLLEKADLVLGV
                     SRTLLAKVESVLQGVHLRRSRQLPNGAPLDVIRAVKAGEAGSDLPPDYLLTAGHLIQR
                     KGIDLVIAALREAKERGVVLQLVVAGDGPERENLIRQSREQGVSDQVRFLGNQSHRQV
                     LSLMKTCLAFVLASRAEGMPLVIAEAMACGKAVIASNIDGVPEIVHDGTTGILVPAED
                     SGALATGLIRLCSDVAFRETLAKQGKEWACREYNWEAIAHRYLGFIEECQAHRQMS"
     gene            complement(12912..13820)
                     /locus_tag="PK614_03440"
     CDS             complement(12912..13820)
                     /locus_tag="PK614_03440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81292.1"
                     /translation="MPRVSVVIPTYNCAKFLGRTIDSALRQTYRDFEIIVVDDGSTDG
                     TQALVAAYEESVRYVYQPNQGASAARNAALSRASGEFIAYLDADDLWRPDKLSRQVEF
                     LDAHSTCGFVHTEVSVIDEQDTVLHTCFNQDTKRPIPQGQCVRDLLLRSHIQTLTVVE
                     RRTAFDDAGKFDPRLPIAQDYLHWIGVGLQGYGVGYLPEPLGQYRWRAGSLMSSQRRL
                     LGDFVKIYEILSTEYGLERSQGPEIMQLVQAQLYATQRQLAYLERRECSGAAARQRLG
                     ALIREWPLNLELYVDFAKTYVSRQVS"
     gene            complement(13847..>14109)
                     /locus_tag="PK614_03445"
     CDS             complement(13847..>14109)
                     /locus_tag="PK614_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81293.1"
                     /translation="AHGLPIIVTRGPLMDKAFVHGKNVLTCEPKDHPAVTKLLLEVMD
                     RADLRERLRSGAVKLAAEWFSWEAATEKTLTALRPRLSDKVV"
BASE COUNT         3600 a         4163 c         3268 g         3078 t
ORIGIN      
        1 cctacccggc gaatggcata cctattttct gggcagctcc cttcgaagcc cattcgcact
       61 gacactgcgg cctcatatcc attagctttc accagatcct caatggaagc gtcgctgttc
      121 ccattcgggt aacaatagac cggcacaaag ttgacaccct gatcaaaaag aacctgtcgt
      181 gacctcacaa gctcttcgga aacctcgtcc aatgtgatgg tcgtcataat acggtgtgtg
      241 caggagtggg aaccgaatga cacccctccc cgagacattt cacgcacctc gtcccaattt
      301 acaatcaccc gatccagcgg taatgtcact cgtagttctg cagccaacgc ctgcaccaat
      361 tctcgtatgc gctctattgg caaatgctta cagcgctcga taatttcgtc ccctagttgc
      421 tcttggcgaa ccgacgcttc aaccaaccga gaagcatctt cacccaggaa accggacaac
      481 acaccctcaa ccgctgccag aggtttctga cgctgtttcc gagccgcaac aaccttacac
      541 aaaaccgcca actgatcagg ccaaaaccac tcgttccttc ctacgtagtc agtgggaagg
      601 aaaatcgtcg caggcatacg cagctgcttc aaaactggat acgcatggcg atagttgtcc
      661 aaccagccat catcgaaggt aatcacgcaa taccgcgcct gggcactcca ctcgccccgt
      721 tgccacagat ccaggagttc ctggagcgac agcactgtaa aattactcct tacaaaagac
      781 atctgttgag caaatacgct gtcaagcaca tacattcctg gctgaaccgg ataacgagcg
      841 acttcatctt tcgtcagcac gcggtgatac attagcaccg ccaccttgcc tcgatgacgc
      901 catcgataga tagatgaagc gtggaaggtc cttgctagcg cagaccgaat tgcttttttt
      961 aaccgtccca ccgagaagtt catctcaaca gctgagtcct cgaaattaga acaacgtgta
     1021 gataaacgga gccaccgctg atccttgcgt cagcacgatc aagctgccca atagcacgag
     1081 catcacaata atcggcaaca gccagaattt tttccgttct ttcataaagg cccacagttc
     1141 tgttaggaac tcgcccatag tgccctccgt ttacttaaaa ttgcctggtc atgtggttgc
     1201 gtggcctcgc ctgccgcacg actcgataac tttcggcatc tcggctcagc gctcttcgca
     1261 tcgagtccca accaaacatc cgcatgatca cccccatcgg ggtgatgagt ccgaagtaca
     1321 gaatccctaa aatgatcctg gtattgatcc atcccatcac gtggccgact ttcatccaca
     1381 ctctaaaaag aggcgcaaga atggtcggcg cggctaatcc caatggaact agcagtgatc
     1441 ccggcacgat ggcccataga cgaatccctt caccatagcg gatcataggc cagacgccga
     1501 tcagaataaa aacaccaccg acaatcaacc caaagttgcg caactctttc ttttcaacca
     1561 tcgtatccat tcagtacccc actgtaacta tgttttccta cgcgactgtg ctgatcaatc
     1621 tgcccatccg cgcttagcgc ttggacggct cccaacgaaa cacccggtct ccccgaaaat
     1681 accgcatcca ggcgtcaatg atggaaaggt tgacaagaac aaaaaacgag ggcaatcgaa
     1741 acacattgct ctttggcatc cagctgcagc cagcataggc aattgcaatg gcataaaata
     1801 aaatctgtcc cagcaaaaga acccggtacg gaccggaact tattgccaag acaatatttg
     1861 agcccagcgt gccgatcatc gcaaatggca ctaaccatcg acatagcttg tggctgaaca
     1921 gttgccaggc aaagatgcca tatcgaagtg ggtttaacat tggcaggctg cgcatcaaca
     1981 cagcaatccc ccttaagacg gtccggactt tgcgctggta ctcctttttc tcatctgtta
     2041 agtttttata gtagcctaca ctcgctggat ccgacacacc acgtagcccg gccttcatcg
     2101 aattcaacaa cgtattaaaa tcgctttgca gatcgtctgc ccaaggacta cagaccgtcc
     2161 tcctggcggc aaagaatgat ccgctgagac cgaccaacgt attcactttt gtttccaaat
     2221 gacgcagcac catctcgtac ttgacatacg cgccctctcc actgagattg ccctgagcat
     2281 cgacaaactg gtccacacta ctaacacagc cgaccgtggg atcactgaac gacttcacaa
     2341 tattggcaat gccctgagga ggcaaagtcg tagcgacgtc cgagaaaacc agtatttctc
     2401 cgttcgtctg gctgaccgcc agtttctgag ccgcttcttt gcctcttcgc tcctgggcac
     2461 gcaccagccg cacacccaaa gccgcatacg atcgtacgat gtcgtcggtc cggtctgacg
     2521 agcaatctga ggccacaaca atttcaaggc gctcagaggg atactgctgc tgaagactat
     2581 tctcaatctt ctctttgatg cgcgcctctt cattataggc cgttattaca aacgaaactt
     2641 tcggctgaat atcgccgacc agcacgggtc tatttctgaa acaggaaagg accagcaaag
     2701 caagcaagta gcctgcatag gcataaatta tgaatatcaa tgaaccccag aagatccatt
     2761 ccacaaacca catgattcag gttcctaccg tttcaacagt ctgccgttgc tcaagccaaa
     2821 atcgcccgat cacctaaata tttcgaggca gctcaggggc atgacgctgc ccgttcgtaa
     2881 tatccggacg acgaatcgtt gctgcgccat caccccgctg agccgcttga ataatagtgg
     2941 aaattcgaac gatgtcggag tcagacacca attcgtgggt tgggagggta acaagccgtt
     3001 cagcgatcat agtcgactgg ggcacatcct gagatgaaag cgtgtcacgg agtttggtaa
     3061 tgtgctgaag agatgaggga tacaggggac tgattccaag cccctgttcg gctgaggtcc
     3121 gacacaccgc atctttttcc tgcctcgaac gcatcagcac aggcaatcgc agatacactg
     3181 actgccctcg tccagacggc ttaatagtct gcacattcga cagggccagc gagcggagga
     3241 tctgctcgga atgagctact cgactcgtgg tcgaattggc tagccgccgt ttccagcgac
     3301 gcagcaatcc ggcgcgtatc gggtccagac gagtaattgg gaaatcagtg taaaactttg
     3361 tctctcccag ctttagaaac ggaagtccag ccggcagcca atagagggaa gggttaagca
     3421 acaccttcgt aactgccacc tcgagccaat tcttcaacat gtcagccagc gacatctcgg
     3481 gaagcagtgc atattcacga gccaagactt cacctatccg atcatcattc gtaagaatgg
     3541 caccccccga accgcacgta atattcttcc ctctcccaaa actcagaaac gaaacatcac
     3601 ccatcgctcc gaacggaact ccatcgcgtt cacctccaaa cgcctgcgcc acatcctcca
     3661 cgacaaaaat gccccgttga cgacacagct cgacgactct agggacatca acgccaattc
     3721 ccagtagatg agtggccaac acgcacaaga cgttggaatc agccacttgg gaaagttgca
     3781 caaagtcgaa atccaacgac agcggatcca cgtcacacag cgccaccgac aatcgagcac
     3841 gaacaacggc cgacggcaca gaaaaacaag tgtatcctgg aagaactacc ttactgcgcc
     3901 cagatagact atgcagtgct cggaagatga ggctgagagc agctttgccc gatgacacaa
     3961 accagacatg cttcacaccg aagtattggc gaaattcagc ctgtagctgc ccaacggttt
     4021 cgtgcgggtg cacgagcccc atcaacccct gaatgagatc tcgccattcg atcggtgcgg
     4081 ccgatggagg aagtgttcgc tgagctttca tggaagatgc ttcacaatgt aagggccaat
     4141 cagattcgcg actaccacag gcaatttctg ccacagacga attgcgagag catatttcgg
     4201 attttgcgga ttgagctgag gaaccggacg cccatctttc atccaatagt accaatacag
     4261 ctgatgtggc tgagctcccc attgctcctt gaatcgatag gttccgctgt cgggacttga
     4321 acggccaaag tcaaagacct gaaagccctg ttggcaggca tattccaaca ccgtgccgta
     4381 gagcaacata tttggagcca gcttgttaaa gcgtttatcc gacgcggccc aaggaatttc
     4441 taaagaagac cggaatccgt ataggaaacc tgcagcaacg ggcgtctgac cgtgggaaac
     4501 aatgcagatg ttcgattcct ttggaaacac ctcaaggatc tttgcaaaga atcccttcgc
     4561 ataaaccggt gtccctaaat cacgcatgca tcgagtgaag actgagtaaa actcatcgag
     4621 acactcactc ccccctaccc gtgccgtcat gccttctttt tgagcccgac ggacctggct
     4681 tcgtaatttg gagggaaatc cctttaccag ctgctcatac gagctcggca acggaagccg
     4741 catagacact tttcgttcgg tggccaccca agccgtgtcc atcagcgcct ggtgtcgaag
     4801 ctcgatatgg tccgcgttta gttctcgagc ttgctcaata gcacgcgctt ccaaaaagcg
     4861 atgtgcttcc gctgaacttg caaccacccc gccgtagttt acgaacggca gggatacgag
     4921 gaaacggcca aaccctcggc ttgccaaaag aaccagcggc agaacgccct gtagtgcacc
     4981 gtcttgcccg tgcacaacca gatagtgggt tggatgccca aaagcctcct caatcacgcg
     5041 gcgccagccg gactgatgat accccgtcgc gaggggagaa cttagcacat aactttccca
     5101 tgcgaggatt tcggcattgg ttcggccaag tattgaagag accatcgtac tcataacgac
     5161 aacactacga acattctttt gccccaatga ccacatcgag tacagctgcc aagtccccgg
     5221 tcaacgagcg ataatcgaaa cggttcacac gcgtcacgtc aggctgagcc agttttcgcg
     5281 ctcctgttgc cagctcgttg agaagcactt tgatcgcggc cagctgattc gggctactta
     5341 cacccagggc gtgccgagtg actagattgg cggttgcccc ctccccaccg atcaataaca
     5401 gcggtcgacc agtcgcaaca tattcgtaaa ttttggccgg tacctgcaaa ggggagtcag
     5461 gctgaagcac caacagcacg ctggatcgtt gcatctcttg taggcacaag ctgtgcgaga
     5521 tcggcggctc gcgtcgcaaa aaagagcgtt tctcgagctc tgctgcatat cgctcacact
     5581 cctgatcggt tgagtcccat ccgcctacga agcgcaatcg gaccgcttcg ggcttcaacc
     5641 gaccctcttg aaataactcc cacattgcct gcagtaagac acgcggagtc ctcttcccgt
     5701 agaccgtccc gaaatgacac agttcgtacc ctgtcgaggg agaaaggcta tccaattgct
     5761 cagcagggat ctgtccgaga ggcaacacgt cccgatcgaa cccgttggga atccaggtac
     5821 agcgacctcg gagttcgcca tattcctcaa tgagtcgttg gcgaagctcc tcggtattag
     5881 caatgacatg actagcagcc ttacaaactc tcgcctcaat cgcttgtgat ttcctggtca
     5941 acaaccgaga actgaaggaa taatacgggt tacaattcca tggatcgcga tagtccagta
     6001 ccagtggccg attgaactgc ttggccaggg ttgagccgac caaaaaacta gtccacggcc
     6061 cacccgtcgc gaaaaccacg tccggaatct gcttttcgtc gaattgttga aaatgccgca
     6121 caacctgccc caaccagcca cattgtcgat ccggaaatgc aaatgcccaa tctagcaaaa
     6181 aatctttcac cgttgaacgg cctgcttccg attgaggcga cgagggaggg gcctgggttg
     6241 tttgtagatt ccctgtccta tcctgcccca cctgtctttt caccattctc ctgagtacgt
     6301 cacgatagta caatatctgc tgaaggcgat cggtataggg cacacgagtc actcctaccg
     6361 tcgccggcac acgttcacca agcttctcat cagctcggtg tgccggatac acacactccg
     6421 gagcggttgt gagcacataa ggctgccacc caaatgttgg aagatttcgg cagaaaccca
     6481 gcgggcgcat tgcaccactc gccgcaaccg gcgggaaata gtaggcgatg atgaggactc
     6541 gcttcacagc gtcaccaccc ctcctccaac gaggccacgt ttgctggata gaccttcggc
     6601 aacctggcga acacgagttt gatcacagtg gagatgaacg atccgttcag aaacatggcc
     6661 atgccacaat aggaaagata ccgacgtcta acggcactca ccaaaaataa actgttaggc
     6721 agcgagacgg gaatcgtgac gcaggagatc tgcaaaaatc tcgaactccg caacgtggaa
     6781 tagcctattg gcgaaatctg actctcctcg gtggtgccgt tccaaatccg ttgtcaaagc
     6841 accaatgtta tagatacctc gctcacgaac ggcctgactc cccaggatat ctcgaagcgg
     6901 ctcatagaga tcatgcgcga accacttctt gctcgctgtg ggaaacccca tcttatccac
     6961 gcgtgatcgc actgactctg ggatccgccc cctcatgcct tctcgaagca catatttgtt
     7021 ccagggccca cgtatcttcc agtcatctga aaggcccgac acgaatgaga ccaggcgata
     7081 gtccaaaaac ggaagccgcg cttccaccga gtgggccatg gagttgcgat cctcaatccg
     7141 taagtacaga ggcaacggcg cggaaacaac tgactgcttc aatgagttcg acaaggtcgt
     7201 agaggccggc gagacatctt cattgatgaa atgcttggtg aggtcctcac taaaccacgg
     7261 attttgacgg agacgtgcct ggcgtcgagc ccgtgcccaa ctgcggtacg cattgaattt
     7321 atacatttcc catgagagac accgggcagc agccgctgca agacgctgac gtgaattgcc
     7381 gccatgagcc tctgtatagg ccgtcacagc ctgccacgct tctcgcactc gcccctgtcg
     7441 aagaagtgat acccaatagt cttggaaata actggaatag cccccaatgg tctcatcagc
     7501 tccctgtccg tttaatacaa cacggattcc attggacgcg gctagacgca tgagctggta
     7561 gcccactacg gcagtcatag tgtgcaccgg ctcgtcttga aaccagagaa gttgtcgcaa
     7621 gtcactccat agctctgcgg gactagtttc gagctgcctg agttgcgcgc gggtttgagt
     7681 caacgtgtcg gtaatatatt tcgactcgtc gaactccttt gccatataac aaaaagcctg
     7741 gagagattcg gactgcccag cagccgtctc acctcgctga cgagccgctg cacaaataat
     7801 cgctgtcgaa tcaagccccc cggacaaaca tactcccact gggacatcgc ttcgcattcg
     7861 aattcgaacc gaatcttcaa acagatctgc gaacgtcgcc gcagggtttt tggatgcact
     7921 cggaggtaac tcatcgagag accaaaaaga ccattgatgc caggtgccat ctagcgtcag
     7981 ttcaaacccg ctccccggag gaatctgctc gataccttgg tagaacgtct caacctggct
     8041 ttccaagcga ccctcgaaca ggaacttggc ggcagtccgc caattgatct cacccggctg
     8101 gtagccagat gctcgcaacg ccttaatctc cgaagcgaat tgcatcacat cggagctacg
     8161 actgtagtac agcggcttga caccaaatcg atcgcgcgag ccaaaaatcc gccgatgtcg
     8221 acgatcatag atcacaaacg cccacatgcc attgagtttg gacaagcact cacgacccca
     8281 ttcacgatat gccgccagca ggacctcgct gtccccgctg gaccgaaact ggtaccccaa
     8341 ttgacgaagt tccgatcgca gttcgacata attgaagatt tcgccgttga agaccagcac
     8401 atactgccca tcctcactaa ccatgggctg atggcccgct tcggacaagt caagaatcga
     8461 aagacgacga aatcccattc cgacagcacc atccagatag atgccaccgt catcggggcc
     8521 ccgatgaagc aaacttttgg ccatccgctc aacggccact cgctcgatgg gccgtccatt
     8581 cagccctatg gcaatcgcaa tcccacacat aataactatc tgctgcccct gccctttcta
     8641 gcctagcgtc accgaacacc cattacttcg caataacctg gcgaaagtac tccgtggcct
     8701 tccgaatacc atcctcgagt tctactttcg gttcccagtc taagagactc ttagctctcg
     8761 taatatccgg ccgacgaacc ttcggatcat ctgctgggag cggacgctct tcgattacac
     8821 tcttggaacc ggtaattttc agaatgatct cagcaatacc acgaaccgta agttctcgtg
     8881 gattcccgat attgaccgga tcatgaatgc tgtccagaat agcgtcgggc ttctgagtga
     8941 ggaacttcgt acgatcagtg cgttccgcca cagacttgtc tgatttcacc atcaataacg
     9001 ccacgatacc acgaaccaaa tcatcgacat agcaaaagct gcgtgtctgg gagccgtctc
     9061 caaagaccgt cagcggtttt ccctgaaggg cctgtacaat gaaattcgag acgacccgac
     9121 catccttcgg cctcatcctt gggccgtagg tattgaatat ccgaacgatt cttgtgtcga
     9181 ctccatgata tcggtgatag gccatcgtca tagcctcagc aaatctcttc gcctcatcat
     9241 agaccccacg cgcgccgatg ggattcacat tgccccagta ggtttccggt tgaggattga
     9301 gcaatgggtc gccatagact tcagaggtgc tcgccaatag aaaccgcgcc cctttggctt
     9361 tcgccaatcc caacgccttg tgtgttccca aggcccccac cttcaaagtc gcaatgggca
     9421 tctcaagata atcctgaggg ctcgccggag acgcaaaatg cattaccgcg tccaattgcc
     9481 catcgacatg caaatagtcg cacacattgt atttcacaaa gctgaatttc ggatttccga
     9541 tcaggtgaga aatattctcc gctcgtccgg tgatcaaatt atccagcgca atcacatcat
     9601 gtccctgccc aatcagcaga tcggataagt gactaccgag aaaccctgct ccacccgtaa
     9661 tcagaattcg catgaatcaa ttcctccttc gccttcacta tcttactggt tagtcgagct
     9721 caaactcttt cttccaatcc gaatcgccct caagcggctt ctgatcggcc ttaagcaaga
     9781 cgcaattctc cagcacgagc acatccattt ccgttcgcat aaaacatcga taggcatctt
     9841 ccggcgtgct gacaattggt tcccccctca cattgaagga agtatttacc aacacggcat
     9901 gccccgtctt tgcttcaaaa gctttcagca acgcatagta ccgtggattt gtgtcctgat
     9961 ggacagtttg aatccgcgct gagtagtcaa ggtgagtcac agcgggaata tcagaccgcg
    10021 ggatattcaa caattcgatc ccccacaatc catcttgcgc ggaactcaca ggaagacgtc
    10081 gtttttctac gacaggagct acgagtagca tataggggct gtcggcatcg aggacgaaat
    10141 agtcggagac tcgttcgcgc aagaccgacg gtgcgaaagg ccgaaacgat tcccgatact
    10201 taatcttaag gttcatcacc gactgcatgt cccgatttcg cgcatcgcct aggatgctac
    10261 ggcctcccaa tgcccgaggt ccaaattcca tacgtccctg tagccaaccc acgaccttcc
    10321 ctgcagcaag ttcttcggct acacgggcat agagttgatt ctcatcgagc ctgacatacg
    10381 gcgcatcaac ctttttcaga tatgcttcaa tttcctcgtt gctatgacag gggcctaaat
    10441 atgatccctt aataccatcc ttgccggaag tcactttgcg cggttgttgt tcatactggt
    10501 gccaggcagt taatgcagca cctaatgcgc cgccggcatc gcccgcggca ggctgtatcc
    10561 agagaccctt gaagggtccc tcccgaagaa tacgcccgtt cccgacacaa ttcagcgcca
    10621 caccaccggc catacacata tagtctactc cggtctcccg atgtagcgtc cgcgacagac
    10681 gtagcatgac ctcttctgtc acttcttgaa ctgaacgagc taaatccatc tcccgctgag
    10741 tcagcttgga ttcaggcttg cgaggtggtc ccccaaaaac atcatcaaac ttgcctccgg
    10801 tcatcgtcag tccggtgcaa taattgaaat aatccatatt gagccggaac gttccatccg
    10861 gcttgaggtc cagcaaatat tcgtaaatcg ctttcacata cttcggctcg ccatagggcg
    10921 ccaaacccat caccttgtac tcgccggaat tcaccttgaa acccgtatag tatgtgaagg
    10981 cggagtacag cagtccgatg gaatgaggga aaggaatttc ccagagggga gtcaacgtgt
    11041 tcccttgccc aagccacgcc gacgtagtgg cccactcccc gaccccgtcc atgcacaaaa
    11101 ccactgcctt ctcataaggt gagggaaaaa aagcagatgc cgcatgcgat tcatggtgct
    11161 ctgaaaacaa aatctgtggc agggaagatg tcgtcaggtc cggggcatgc tggagaaatt
    11221 ccttctgcag caaactcttc aacagaagct tctctttcag ccacaccggc atggcggcca
    11281 cgaaggattg aacaccccta ggcgcaaaag caagataggt ttctagcaat cgctcaaact
    11341 tcaccagcgg cttgtcatag aagacgaggt gccgcacatc tttgagctct atctgtcctt
    11401 cacgaagaca gtagtcgatg gctctatgag gaaacccggg atcatgtttc ttcctcgtga
    11461 agcgctcttc ctgagccgca gcaatgattt ccccatcacg caccaaacat gcggcactgt
    11521 cgtggtagaa cgcagaaata ccaagaatat tcatcgctgt tccccgcctg taaaagaaat
    11581 gtgtctctgc actaatctag catcgtttaa gcaatcccac agtcctctgt cagagggggt
    11641 agcccctgct tttactgaga tggttataat catgacattt gccggtgggc ctggcactcc
    11701 tcgatgaagc caaggtatcg atgcgcaatc gcttcccagt tgtattctcg acaggcccac
    11761 tcttttccct gcttagctag agtctctcga aaagctacgt cagagcacaa cctgatcaga
    11821 cctgttgcga gagctccaga atcctctgct ggtaccagaa ttccggttgt gccgtcatga
    11881 acaatttccg gcacaccgtc aatattgctc gcaatgacag ctttcccaca tgccatcgcc
    11941 tctgcgatga ccaagggcat cccctctgct cgcgaggcca gcacgaaagc aaggcacgtc
    12001 ttcatcagac tgagaacctg cctatgggat tgattgccca aaaaccgaac ctgatctgac
    12061 acaccctgct cgcgtgactg gcgtatcaaa ttttcccgtt ctggtccatc acctgcaaca
    12121 acgagctgca acaccacacc gcgctctttc gcttcccgca atgcggcgat cacgaggtct
    12181 atccccttcc gctgaatcag gtggcctgcg gtcaatagat agtctggagg tagatcagaa
    12241 cccgcctcgc ctgctttaac cgcccggata acgtcaagcg gtgcgccatt gggaagctgc
    12301 cgactgcgcc taagatggac gccctgcaaa actgattcaa ctttggccaa gagcgttcgt
    12361 gagacaccca acaccaggtc agccttttcc aataagaacc ggaccaatcg ttgttctcgc
    12421 cgatcccata gacataagtc atgcgcatcg tttccttgat aggtgatgaa aagagttcct
    12481 ccgaatacac gcttcaatat cccaaaatag aacatcgcag gaagcggata ctgaattaaa
    12541 actgcatcca gccgtttctt cctagccagc cagatgagtt gcaccatcgt aatcggaaac
    12601 caaatacaac acatgaccat ggcacgaaga ggatactgcg acgagacagg tgaccggaga
    12661 tacatgccgt agactgggat ttcatccagt gcttcaattt gacgaagccg attctcgcga
    12721 tcggcgacaa aaatggtgat attgtgacct tttttctgaa attcccccgt caaccgtgcg
    12781 acaacggtac tgatcccccc ggtgagggaa ggtctccaag gagtgaccag cataatgttc
    12841 atagttgaat tgcacctact cctacaaacc gcctatctgc gttcgctgtt gaaccagagg
    12901 attcgctgcc gtcaggacac ttgccgactg acataggtct tggcgaagtc cacatacagc
    12961 tcaagattta ggggccactc tcggattagg gcaccaagcc gttgccgcgc tgctgcacct
    13021 gaacattccc gtcgctcgag atacgccagt tgccgctgag ttgcgtacag ctgcgcttgc
    13081 accagttgca tgatctcagg cccctgcgac cgttcaagac catactccgt cgagaggatt
    13141 tcgtatatct tcacaaaatc tcccagaagc cgacgctggc tggacatgag actccccgcc
    13201 ctccagcgat attggcccag cggttcaggc agatatccaa ctccataccc ctgcaggccc
    13261 acaccaatcc aatgcaaata atcctgagca atcggcaggc gcggatcaaa tttccccgca
    13321 tcgtcaaatg cggtgcgacg ctctaccacc gtcaacgtct ggatatgaga ccgcagcaac
    13381 agatccctga cacactgccc ctgaggaata ggtcttttgg tgtcctgatt aaagcaggtg
    13441 tgcaggactg tgtcctgttc atcgattacc gacacctctg tatgcacaaa tccgcaggtg
    13501 gaatgcgcat caagaaattc gacctgccga gacaacttgt cggggcgcca aagatcatcg
    13561 gcatccaggt aagcgataaa ttctccgctc gctctcgaca gggctgcatt ccgtgccgcc
    13621 gaagcaccct gattgggttg atagacgtac cgtaccgact cttcgtaggc cgctaccaaa
    13681 gcttgggtgc catccgtcga tccatcgtcg acgacaatga tttcgaaatc tcgataggtc
    13741 tgtctcaacg ccgagtcgat agtccgccca agaaatttgg cacagttata agtcggaatt
    13801 acgacggata cacgaggcat gtgggaatgt acccaggaaa agcgacctat acaaccttgt
    13861 ccgacagccg cggtcgcaaa gcggtcagtg ttttctccgt cgccgcctcc caggaaaacc
    13921 actcggcggc gagcttgaca gcaccagatc gaagacgctc acgaagatcc gcacggtcca
    13981 tcacttcaag caggagtttc gtcacggccg gatgatcttt tggttcgcag gtaagaacat
    14041 ttttcccatg gacaaatgcc ttgtccatca gaggaccccg tgtgacgatg atgggaagcc
    14101 cgtgcgcaa
//
LOCUS       DAORQN010000030        13863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_279_length_13863_cov_51.224508, whole genome shotgun sequence.
ACCESSION   DAORQN010000030 DAORQN010000000
VERSION     DAORQN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13863
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_13863_cov_51.224508"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PK614_03450"
     CDS             complement(<1..728)
                     /locus_tag="PK614_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRB81294.1"
                     /translation="MASVLLNKRIEFCASHRYHKPEWDAAKNRATFGPCNNDPGHGHN
                     YMLEVTVAGEVDQRTGMVVNLFDLKRVLLQVLEEFDHKHLNLDLPYFERQIPTSENVA
                     RVLWDKLQAQPDIGTLQRLSLYEDEDLCADLTAEAGPEVASVTRRYSFTAVHDGHRGH
                     TWDLFVSVYGPIDRETGMVTDIVALDHLLKERVLIPFDGRDLRIVLATPSVTGEYLAK
                     AVWDRIASAIPAGTLQLVKLVQTR"
     gene            complement(802..1392)
                     /locus_tag="PK614_03455"
     CDS             complement(802..1392)
                     /locus_tag="PK614_03455"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HRB81295.1"
                     /translation="MKALTQQDLIPHDEYERRRETYRQAIIEIKRRRRIGLGEKITLV
                     FENRETLRFQIQEMIRVERIFDPQKVQEELDVYNALLPASGELSATLLIELTDPDTMK
                     QWLDLFMGLDHGQKVGLRAGGDVVYGEFEGGHSHDTKISAVHFVRFRPTPAMVTAIGD
                     QAARVALTVRHAGYEAEAEVPWVMRQEWLEDLSTVG"
     gene            complement(1445..2731)
                     /locus_tag="PK614_03460"
     CDS             complement(1445..2731)
                     /locus_tag="PK614_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRB81296.1"
                     /translation="MNQFNLINPIDPKDLEKETLRIYDVCDGCRRCFNLCPSFNTLLD
                     RIDEYESDVAKFTATDHHRVVDECYYCKLCFNHCPYTPPHQYGIDFPLLMAVWKKRLA
                     TERGTRWRDWLLTRTDLLGRVNSAFAPLVNWGLGQGWLRGLLQTVLGVHRDRQVLHYQ
                     RETFVRWWARRSAGRKVGTGKKAALFAGCLVNYQATDVGKATVQVLEKNGVEVALPDQ
                     SCCGMPSFDIGDTAAMVAAAERTIASLKSWVDQGYDVVIPTPSCSLMVKREYVSLVAG
                     DEAKRVAEHTYDISEYLMKMKREGGLAMDFTQKPGRVAYQVPCHLRDQNIGFKSKELM
                     ECAGAKVEVIEKCSGHDGSWSAKVEFFPLSMKIAQKAVRVVEQAPADVVASDCPLAGL
                     QLDQAGAGAHAGGKSSQHPIQIVRDAYGLPRDPRAS"
     gene            complement(2841..3269)
                     /locus_tag="PK614_03465"
     CDS             complement(2841..3269)
                     /locus_tag="PK614_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRB81297.1"
                     /translation="MGNSLKGTKSHENLKHAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFSELAEWFETLAKAERSHANRFTKGLDSLKQG"
     gene            complement(3306..3758)
                     /locus_tag="PK614_03470"
     CDS             complement(3306..3758)
                     /locus_tag="PK614_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRB81298.1"
                     /translation="MKLNSQEIEARFRRHAVRVTRQRAAIYAALAETTSHPTAEDLYR
                     TVKREHPMMSRNTVYYTLGVLRQAGLVQEVNVGHEVARFDGNVTVHHHLICLGCGQIV
                     DVMDDGLNHLAIPSRQAGGFHVLGHRVEFHGYCADCQQAGLGAMERRG"
     gene            complement(3989..4546)
                     /gene="rfbC"
                     /locus_tag="PK614_03475"
     CDS             complement(3989..4546)
                     /gene="rfbC"
                     /locus_tag="PK614_03475"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRB81299.1"
                     /translation="MQITPTPLKGLFQIEPDVFGDARGKFVEIFRESRYDAAGIDKPF
                     VQDNFSWSVRGTLRGLHYQLSRPQGKLVTVVKGTVYDVAVDIRHGSPTFGQWYGVELS
                     DSNMRQLYIPPGFAHGFCVLSEDAGFLYKCTDVYSPPDERGILWNDPALAIAWPVKTP
                     LLSAKDQAYKCLADMMTELPRYESR"
     gene            complement(4678..5049)
                     /locus_tag="PK614_03480"
     CDS             complement(4678..5049)
                     /locus_tag="PK614_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81300.1"
                     /translation="MPDLQFALVVSALCTSGLETLNVQEELRRRVFDACWALVSTDPP
                     PTNPRERVLDLRFGTELTLDAIVATIRQLFAEAGISTLTWDHAPSDPTRPSSPAAEPL
                     IDRLQRLYPDPPPTADPSDRN"
     gene            5184..5456
                     /locus_tag="PK614_03485"
     CDS             5184..5456
                     /locus_tag="PK614_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB81301.1"
                     /translation="MITITLMGQLQTSDGERDLACELPTPVTVRQVIQRQGRGLRHML
                     QLLREKKVLVTINKRIASEDSLVQDGDAVRFVGHDGAGGSGLAPSF"
     gene            complement(5571..5858)
                     /locus_tag="PK614_03490"
     CDS             complement(5571..5858)
                     /locus_tag="PK614_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRB81302.1"
                     /translation="MAKSMTKSQIADHLAGKAGITKKTAVQFLDDFAALAYREAKNAF
                     VVPGIGKLVLANRKARMGRNPQTGEPIKIPAKRVVKFRVAKMAKDSILGKK"
     gene            6154..6711
                     /locus_tag="PK614_03495"
     CDS             6154..6711
                     /locus_tag="PK614_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81303.1"
                     /translation="MAKELPILPSNAQPAEGAAPEAVIYSRVFCKNDNAPPLRLLIEF
                     LKSRGQLPILPPDMSDAMLDEWAWVQVVLGYARERKPIHVFCVRDRGSYQDLFEQEQK
                     QFLEHLSSHDSLEASLAQEHVTRARFILTTRMVEADVTDEGYDFNGWILEFFQEHCNG
                     IVQIDQQGFFSPKGELIVDLSLPEE"
     gene            complement(6981..7160)
                     /locus_tag="PK614_03500"
     CDS             complement(6981..7160)
                     /locus_tag="PK614_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81304.1"
                     /translation="MDYPVRFAGNAHRAACDGSVGVNTAGSVGLQGGAWKVGESFGHL
                     IDGVICPPVKDRFRF"
     gene            7394..7470
                     /locus_tag="PK614_03505"
     tRNA            7394..7470
                     /locus_tag="PK614_03505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7428..7430,aa:Arg,seq:ccg)
     gene            7518..7593
                     /locus_tag="PK614_03510"
     tRNA            7518..7593
                     /locus_tag="PK614_03510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7551..7553,aa:Lys,seq:ctt)
     gene            7845..8183
                     /locus_tag="PK614_03515"
     CDS             7845..8183
                     /locus_tag="PK614_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81305.1"
                     /translation="MAKVIRLEVRKLSLAEDPGEDLSKVSLVQHAPLFIDKQPLRLFF
                     PPGNGLRVQPRQVVAQRASQPAAQVNKPRLFCLRRFNLSIQIQRSLNPNGPPRHVQIG
                     GAQRHELAGP"
     gene            8532..8807
                     /locus_tag="PK614_03520"
     CDS             8532..8807
                     /locus_tag="PK614_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81306.1"
                     /translation="MQLEQLAVAADGALRKLILHRGQPPAGILPQQNVTIREVTGELA
                     RLLNLLCELILSLCPSATADLPDPLTGRVPEIDDPHITVFSLPDYRH"
     gene            complement(8804..8995)
                     /locus_tag="PK614_03525"
     CDS             complement(8804..8995)
                     /locus_tag="PK614_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81307.1"
                     /translation="MDGKELRRRRDQMKLTQVELAEELGVTSNTVARYERDELAIPEP
                     VARLTVLIQKLKAEKRRRP"
     gene            complement(9042..9365)
                     /locus_tag="PK614_03530"
     CDS             complement(9042..9365)
                     /locus_tag="PK614_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81308.1"
                     /translation="MKNLLSELEAVIGSAPADHLPELLGHLERLKGQAWARMMSASLT
                     KNVGPSPDQQLLTLPQVAERLRIPESRAYELARQHKLPAVRIGKYVRVPLGKLQEYEK
                     SLPRI"
     gene            complement(9372..9650)
                     /locus_tag="PK614_03535"
     CDS             complement(9372..9650)
                     /locus_tag="PK614_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81309.1"
                     /translation="MEAASEQHSQSLPQEVPDDVLIMRLRHWASELTPQEALNVLATC
                     SAESLIAYLRSQNRPMPSDPFKLWHLFAAELLARTESMNASHGAVGTA"
     gene            complement(9658..10401)
                     /locus_tag="PK614_03540"
     CDS             complement(9658..10401)
                     /locus_tag="PK614_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81310.1"
                     /translation="MEPFTMMALGGGAASIGGGIAKAFGGDSQAKAARKAAQMYIDYQ
                     NREREKFLGATSPIRDKLMTYSQGKSGYDEDLMRGMKDDVNEDYGQGLADVSRISANS
                     AVGKNSGGGNVYTPGRYDRATRVLGQNLAANKAKMIRDVNQKNADMAVSNQRFAVSAL
                     PTYTPGLSATPQIGYDAFQGLNAVAPVGNYIGDMLQSVGQPAMSAGMDGLVRGPIAEK
                     LAKIQAQNSQQQNQPGPWQSYYTSPYLRY"
     gene            complement(10404..10808)
                     /locus_tag="PK614_03545"
     CDS             complement(10404..10808)
                     /locus_tag="PK614_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81311.1"
                     /translation="MTTPRRELPSRRNHIIQKARISGRRTIYISVHDDASPGEIFLRV
                     KGTDCSAETVGLYDVLARVASLALQYGAPLEKVGEMLLGSKFEPAGPVSLHPRIKNCT
                     SLPDLIGRHVLVEYCNRADLAHVPEPDPDKEG"
     gene            complement(10810..12525)
                     /locus_tag="PK614_03550"
     CDS             complement(10810..12525)
                     /locus_tag="PK614_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRB81312.1"
                     /translation="MKTFEDFQIDIGGRTGVEVQTTCPQCSHTRKKSKVRCLSVNTVE
                     GVWVCHHCGWAGSLRSGADAPSRPLKRVVKPHFEKPSTVPPAVRDWFEKRGISETVVA
                     RHCITLQHAYLPQLEDEAPCVVFPYFRQGEAVNLKYRSLEGKDFRQVGGAEKVLYGLD
                     DLTEDWALIVEGECDKLALEVAGICNAVSVPDGAPPANSKPSDAKFGYLTNCAAHLDR
                     LKKIVLAVDNDAPGHTLEAELARRLGPERCFRVRWPEGCKDANEVLIQSGAEELRRCI
                     AEAKPYPLEGVLQVADLAGDVMSLYQEGLPGGVSTGWPSVDQHYTVRPGEMTIVTGIP
                     SHGKSQFLDALTVNLSRQHEWMIAVCSPENLPVSRHIAKLVEQYTGWPFRQGPTRRMT
                     PHELVMALDWLHGHFVFIAPDEALTIPALLQHAKALVARHGIRGLILDPWNEFDHARG
                     TTTETEYISTSLTQIRRFARNYGVHVWLVAHPQKLYRRDDGSYPVPTPYDISGSAHWR
                     NKADNCLAVWRDENDPDEPVHLYVQKVRFREVGKIGLVPLRWSSINGRYTEALPELAS
                     RWGNS"
     gene            complement(12522..13184)
                     /locus_tag="PK614_03555"
     CDS             complement(12522..13184)
                     /locus_tag="PK614_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81313.1"
                     /translation="MSIRVMSHVWKQSRAKGGDLLVLLAIADFADDSGTAFPSIATLG
                     LKSRLDGRNVQRSIRRLITAGELTLSERQGPHGTNLYQVANCHPANCHPGILPGGVCD
                     MGPLAPAPPNPLKRTISKNIYSPKGDKLPEESCTRLGKFWAAYPRKEGKGACIRWWVA
                     HTPDDSLLDRMLAKIAEAKQTTKWREQGGKFIPMPATWLNQARWDDEYIQSVKRKEPL
                     PL"
     gene            complement(13181..13405)
                     /locus_tag="PK614_03560"
     CDS             complement(13181..13405)
                     /locus_tag="PK614_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81314.1"
                     /translation="MRRTTDNSLYATRGGQTEPVGASEFTPVGSVLGGVLKEIARRVE
                     LRQRLEGEQGVPISDEAFLEIAESTGGVRL"
BASE COUNT         2921 a         4306 c         3675 g         2961 t
ORIGIN      
        1 tcgcgagttt ggacgagctt gacgagttga agagttccgg ctggaatggc cgatgcgata
       61 cgatcccaca ccgccttggc cagatattcc ccggtgactg agggagtcgc caacacaatg
      121 cgcagatccc gcccgtcgaa cgggatcaat acgcgctctt tcagcaggtg atccaacgcg
      181 acaatatccg tgaccatgcc ggtctcacga tcgatcgggc catacaccga cacgaacaga
      241 tcccaggtat gtccacgatg gccgtcatgc acggcggtaa acgaatacct cctcgtcacc
      301 gaggccacct ccggcccggc ctctgccgtg aggtcggcac agagatcttc atcctcgtac
      361 aacgacagtc gttggagggt accgatatcc ggctgcgctt gaagcttgtc ccacaggaca
      421 cgcgcgacgt tttcggaggt tggaatttgg cgctcaaaat agggcaggtc gagattgagg
      481 tgcttgtgat cgaactcctc caacacctgc aacaacacgc gtttcaaatc aaagaggttc
      541 acgaccattc cggtccgctg atcgacctct cccgccaccg tcacttccag catgtagtta
      601 tggccatgcc caggatcgtt attacagggg ccgaacgtgg cgcggttctt cgcagcatcc
      661 cactccggct tgtggtaccg gtgggacgcg cagaactcaa tgcgtttgtt gaggagaact
      721 gaggccatag gaaccgattc tcgtttgaca gcgttcagtc acgattattg tgggtgcgca
      781 tcgcgcacac atccatgaac attacccgac cgtgctcaaa tcctcaagcc attcctgtcg
      841 catcacccac ggcacctcgg cttcggcctc atacccggca tgacgaactg tcagcgccac
      901 ccgcgcagcc tgatcaccga tggccgttac catcgcaggg gtcggacgaa agcggacgaa
      961 atgaaccgcg ctgatcttcg tgtcatggct atggccgccc tcgaattcgc catagaccac
     1021 gtcccccccg gcccggagac cgaccttctg gccgtgatcc aggcccataa acaaatcgag
     1081 ccactgcttc atcgtgtcgg gatccgtgag ttcgatcagg agggtcgcgc tcaattcacc
     1141 cgacgccggg agaagggcat tgtacacatc cagctcctcc tggacctttt gcgggtcgaa
     1201 gatccgctcg acgcgaatca tttcctggat ctggaaacgc agagtttccc gattctcgaa
     1261 gaccaacgtg atcttctcgc caagaccgat ccgcctacgc cgtttgatct cgatgatggc
     1321 ctgacgatac gtttcgcgcc gccgctcata ctcatcgtga ggaatcaggt cctgctgtgt
     1381 gagtgctttc ataatctagt ccggggtgag aagacatctc ctgccgcgag caggaggcca
     1441 accctcagga ggcacgcggg tccctgggca atccataggc gtcgcgcaca atctggatgg
     1501 gatgctgcga ggatttccct cccgcgtggg ctccggcccc ggcctgatca agctgaagcc
     1561 ctgccaaggg acagtcggac gccaccacat cggccggcgc ctgctcgacc acacgcacgg
     1621 ctttttgcgc gatcttcatg gaaaggggga aaaattcgac cttggccgac caggaaccgt
     1681 cgtgcccgga gcatttctct atgacctcga ccttcgcccc ggcgcattcc atgagttcct
     1741 tcgatttgaa accgatattt tgatcccgca gatgacaggg cacctggtag gccacacggc
     1801 caggcttctg tgtgaaatcc atggccaagc ccccctcacg cttcatcttc atcaggtact
     1861 cgctaatgtc gtacgtatgc tcggccaccc gtttcgcctc gtctccggcc accaaactga
     1921 catactcccg cttcaccatg agactgcaac tgggagtagg aatgaccaca tcatagccct
     1981 gatccaccca tgatttcagt gaggcgatcg tacgctctgc cgccgccacc atcgccgctg
     2041 tatcgccgat atcgaatgac ggcattccgc aacaggactg gtccggcagt gccacttcaa
     2101 ccccgttctt ttcgagcacc tgcacggtcg ctttgcccac atcggtagcc tggtaattaa
     2161 ccaagcagcc ggcaaacaat gccgctttct ttccagtgcc taccttgcgg cctgcagatc
     2221 gacgagccca ccagcgcaca aatgtttcac gctgatagtg caacacctgc ctgtcccggt
     2281 ggacacccag caccgtctgc agaagacccc gtagccaccc ctgacccaac ccccagttga
     2341 ccaacggtgc gaaggcgctg ttcactctcc ccagcagatc ggtccgcgtc aacagccagt
     2401 cccgccagcg ggtgccccgt tccgttgcca gccgcttctt ccagaccgcc attaagaggg
     2461 gaaagtcgat gccatattga tggggcggcg tatacggaca atggttgaag cagagcttgc
     2521 agtaataaca ttcgtcgacg acgcgatgat ggtccgtcgc ggtaaatttc gccacatcgc
     2581 tctcgtactc atcgattcga tccagcaacg tattgaatga ggggcagaga ttgaaacacc
     2641 ggcggcagcc gtcgcacaca tcgtagatcc gcagggtttc cttttccagg tccttgggat
     2701 cgatcggatt gatgaggttg aattggttca taggcgcgtt gccatcaggc aggcagccat
     2761 cggcaaccag caatcagcgg aggagatcac atcgatccct cgctgatcgc cgatatccaa
     2821 agactgacgt cgaacaggtc ttagccttgc ttcaagctat ccaggccttt ggtaaaccga
     2881 ttggcatggg accgttccgc cttggccagg gtctcaaacc actccgccaa ttcagagaag
     2941 ccttcatcgc gtgcggtctt ggccataccc ggatacatct gcgtgtattc gtaggtttcc
     3001 ccttcgatcg cggacttcaa gttggcttcg gtgtttccga tcggcacacc ggtggcggga
     3061 tcacccactt ctttcagaaa atccagatgg ccgaatgcat gaccggtttc cgcttcagac
     3121 gtatcacgga acagcccgcc cacatcggga tacccttcga tgtctgcacg gcgcgcaaaa
     3181 tagagatagc gccggttggc ttgtgattcc ccggcaaacg cgtgcttaag attctcgtga
     3241 ctcttcgtgc ccttcaaact gtttcccata ctctcctcct tcagttaagt gacgaatcga
     3301 tactcctacc cacgacgctc catcgcgcca agacctgcct gctgacagtc tgcacaatat
     3361 ccgtgaaact ccactcgatg cccgagtaca tgaaatcccc cggcctgccg actcgggatg
     3421 gccagatgat tcaacccgtc gtccatcaca tccacaatct gaccacagcc cagacaaatc
     3481 agatgatggt gcaccgtgac attcccgtcg aaccgcgcca cctcatgacc gacgtttact
     3541 tcctgcacca aaccggcttg ccgcaagacc cccagcgtgt aatacaccgt attgcgtgac
     3601 atcatcggat gctcgcgctt gaccgtgcga tagaggtcct cggcagtggg atggctggta
     3661 gtctccgcca acgccgcata gatggccgcc cgctggcgag tcacacgcac cgcgtgccgt
     3721 cgaaaccggg cctcaatctc ctgcgaattc aacttcatga tctcgctcac taaacaggaa
     3781 tgattcctac atagcatgca ggcccatcgc gcggcaagac tggctcggtc tcaggcatcg
     3841 gaaggaaggc cacaaagtac ctgagtccat gattccggac gacgagcgaa aggaagggga
     3901 agagaagaca cgggcaacac gttgccggag acccgcctaa gaaaaagagt tgtgtggcca
     3961 tggaatcggc cgaccaaacg gccgaggact agcggctttc ataacgaggc aattccgtca
     4021 tcatgtcggc caggcactta tacgcttgat ccttcgccga cagcagcggg gtctttacag
     4081 gccaggcaat ggcgagagcc ggatcattcc ataggatgcc ccgctcatcc ggcggggaat
     4141 agacatccgt acacttatac agaaaccctg cgtcctcgct gaggacgcaa aagccatggg
     4201 caaaaccggg aggaatatac aactgccgca tgttgctatc ggatagctcg actccatacc
     4261 actgcccgaa cgtcggcgac ccgtgccgaa tatcgaccgc cacatcgtaa accgttccct
     4321 tgacgaccgt gaccaatttc ccttgcggcc gagatagctg atagtggagt ccgcgcaacg
     4381 tgccacgtac ggaccaggaa aagttgtcct gcacaaacgg tttgtcgatt ccggccgcgt
     4441 cgtagcgtga ttcacgaaag atctcgacga attttccccg ggcatcgccg aacacatcag
     4501 gctcgatctg aaagaggccc ttgagtggcg tgggcgtaat ctgcatgaca cctcctgaat
     4561 cgcattggag aaaacggatg gcatcatacc tcgttcgctc tcaggcggaa agcggacctc
     4621 ccacctctcc aacctgatgt tgaatgaacg aagccaataa agaatcgaga agggtagcta
     4681 gtttcggtct gagggatcag cagtcggagg cggatcggga tagagcctct gcaatcgatc
     4741 aatcaacggc tctgcagcag gactgctcgg tcgggttgga tcgctgggag cgtgatccca
     4801 ggtcagcgtg gaaatccccg cttctgcgaa gagctgacga atggtggcca cgatggcgtc
     4861 cagcgtgagt tccgtgccga atcgcaagtc cagcacacgt tctcgagggt tagtcggtgg
     4921 gggatcggtg ctgacgagcg cccaacaggc gtcgaacact ctgcgccgaa gttcctcctg
     4981 gacattcagg gtttccagac cggacgtaca caaggccgat accaccaagg caaactggag
     5041 gtccggcatt cctggtcgct ggatattcag ggattgcatg gtgataatat tagtcgcagc
     5101 cggctggggg agtcaatttg ggccgctcgg ctgcacggca caccggaaca ctacggcaga
     5161 tcgactggga ggacacaggt cgcatgatta cgattacact gatgggacaa cttcaaacct
     5221 cggatggaga gcgggacctc gcctgtgagc tccccacccc tgtgacagtc cgccaggtta
     5281 ttcagcggca aggacgcggg cttcgtcaca tgctccaatt gttgcgggag aaaaaagtcc
     5341 tggtcaccat caacaaacgc atcgccagcg aagattctct agtgcaagac ggcgatgcgg
     5401 tgcgcttcgt gggacatgac ggcgcaggtg gaagcggact cgccccgtct ttttagtatc
     5461 caccgcgtac tcactggcac gccgctacct atggccgctg caacaacaca aaggccggtg
     5521 ctctcaatga cggagcaccg gcccttcagt ctatagagga acgaggctga ctacttcttt
     5581 ccgaggatcg aatctttggc catcttcgcg actcggaact tcaccacccg ctttgccgga
     5641 atcttgatcg gctcacccgt ttgcgggttc cgacccatgc gggccttccg attggccagc
     5701 accaactttc cgattcccgg tactacgaag gcattcttcg cttcacgata ggcgagagcg
     5761 gcaaaatcgt ccaggaactg gacagccgtc tttttggtaa tccccgcctt tccagcaaga
     5821 tggtcagcaa tctgcgattt cgtcatcgac ttggccatgc gttcctccct ttgaacaacg
     5881 aatgaaagtg aactgacttg tgcggtctgc gatctataaa ctcgtacgta cagaggtgga
     5941 gccggaaatc tgcgagtgtc ctgcagaatc agtccgaaaa cagggcgaga gtgtatccaa
     6001 agccctgggg gctgtcaaga gccgcaaagc ggagcacgcg catccagaca ggcgaccagc
     6061 cactcgatgc tcttgcgcgc cgcgtgagcc acagagtaca gtacgaaggc aggttgctgt
     6121 ctattgcatc actcaggatc acgaggcgtc gccatggcta aagaacttcc tattcttccc
     6181 tctaacgccc aaccggccga gggcgccgca cccgaagcgg tcatttactc acgcgtcttt
     6241 tgcaaaaatg acaatgctcc accccttcgc ctgttgatcg aattcctcaa atcgcgcgga
     6301 caacttccca ttctcccccc cgacatgagc gatgccatgc tggacgagtg ggcatgggtg
     6361 caggtcgttt tgggctatgc gcgcgagcgt aagccgattc acgtgttttg cgtgcgcgac
     6421 cgggggagct atcaagattt gttcgagcag gagcagaagc aatttctgga gcatctctcc
     6481 tcacatgaca gcctcgaggc cagtctggcc caagaacacg tcacccgtgc gcgattcatc
     6541 ctcacgacgc gcatggtcga agccgacgtc accgacgagg gctatgattt caacgggtgg
     6601 attttggaat ttttccagga acactgtaac ggcatcgtgc agatcgatca gcaaggattc
     6661 ttctccccca aaggagaact catcgtcgat ctctctctcc ccgaggaata ggcaccatgg
     6721 ggaccacgcc gtcctcccct caccccttgc ccctcttcca gcaagccgag gactggttcc
     6781 aacgtgcacg agcctcactg ctggggcaga tcccctgcgg gcgcggctgc tgcgaatgct
     6841 gtaccggcat ctttcccatc actcgcctcg acgccctcga aatacaacgc ggccttgatg
     6901 aattaccttc tgctcttacg cacgccatcg tcacgcgggc ccgtacccaa atcaccgctg
     6961 tggaaacggc ctacccgagg ctaaaatcga aaccggtcct tgacgggtgg gcagatgaca
     7021 ccatcgatga gatggccgaa cgattcgccc accttccatg ccccgccctg gaggccgacg
     7081 gaacctgcgg tatttacacc cacagaccca tcacatgccg cacgatgggc attcccggcg
     7141 aatcggacgg gatagtccat ggtgcttgtg ccgtccaaac cgccgtgccc attgtccggc
     7201 tctcagccac ccttcgacgc gacgccgacc gcctagctga acacgaggca ctctctttgt
     7261 ccatcctgcg ccgccctcaa tcttcaggcg acgacgaaat cctcctgccc tatggattcg
     7321 tgccaaatgg tgatccaact ccctagacag caacagaaac gctatgctaa tgtgtcggat
     7381 ctgatcgacg ggagcgcctg tagctcagct ggatagagca tcagcctccg gagctgaggg
     7441 ccacaggttc aaatcctgtc aggcgcacca ttcgctctgt caggtacgtt acgatcgaaa
     7501 catagtgcac acaggtgggg ccgttagctc agttggtaga gcagctgact cttaatcagc
     7561 gggccgcagg ttcgacccct gcacggccca ccattttctt ccttaacttt ctcaaacact
     7621 tgcgattctg gccgggctga ctcggccgtc atttctaccg ttggtgccaa cttttggtgc
     7681 caaacttttt caaactcccg atccagcaca tcgacatagc gtcgattccc gctcggcatc
     7741 cacttcgcgt agtgtttcaa cgtcgtcgtc ggcttcgagt gtccgagctg ttgagagacg
     7801 tacaccaacg gcacggcctc agacagcagc aacgaggcgt aggtgtggcg aaggtcatac
     7861 ggcttgaagt gcggaagctc agccttgcgg aggatccggg cgaagacctt tcgaaggtat
     7921 cgctcgttca gcacgctccc cttttcatcg acaaacaacc actccgcttg ttctttcccc
     7981 cgggcaatgg cctcagagtc cagccacgtc aggtagtcgc tcaacgagcg agtcagccgg
     8041 ccgctcaggt caacaagccg cgtctcttct gtcttcgtcg ctttaatctg tccatccaga
     8101 tccaacgatc gctcaacccg aacggtccgc cccggcatgt ccagatcggt ggggcgcagc
     8161 gccatgagct cgctgggccg taaccctgtc cccgccatca ccaagaatcc catcccaaca
     8221 ccaggaggca agagcccatc ggtcaccaag cgctcaaccg tgtgcttgaa tcgggccaac
     8281 tgttcgtggc tcatcgggtt cacgtcagac tgtcgagagc tgccagctgt gcgggactcg
     8341 cgaaaccggc cgagcgccgg gttcactgta atgatctctt cttcaacggc ttgagaaaga
     8401 acggtcgaga gtgccgcttt gatgagacgg acgctatttt ttccgagagt ctggcgcttc
     8461 tctgcgagga gccttttcac atcgcgtcgg cgaagctccc gcaccttcat acttccaagc
     8521 acggggacga tatgcagctc gaacagctgg ctgtagctgc ggatggtgcg ttgcgcaagc
     8581 tgatcctgca cagaggccag ccaccggccg gcatactccc tcaacagaat gtcacgatcc
     8641 gcgaggttac cggagaactc gcgcgactcc ttaatcttct ctgcgagctt attctcagcc
     8701 tctgcccgag tgctaccgcc gacctcccgg acccgcttac cggtcgcgtc ccggaaatcg
     8761 acgatccaca catcacggtc ttttcgcttc cagactatcg acattagggc ctccgccgtt
     8821 tctcagcctt gagcttctgt atcagcaccg tgagccgcgc aaccggttct ggaatcgcca
     8881 attcatcccg ctcatagcgc gcgaccgtat tggacgttac acccagttct tcggctaact
     8941 ccacctgggt tagcttcatt tgatcgcgcc gcctccgtaa ctcttttcca tccatacaat
     9001 acctatacta tcagtatata acattgtcaa gaggtattct gtcagatccg aggaagggac
     9061 ttttcatact cctgaagctt cccgagcggg actcgcacat acttcccgat cctgaccgcg
     9121 ggcaacttgt gttgccgggc cagttcatac gcgcgcgatt ccggaatcct taagcgttcg
     9181 gccacctgag gaagcgtaag aagttgctgg tccggggatg ggccaacatt tttcgtcaaa
     9241 gaagccgaca tcatccgagc ccaggcttgc cccttcagtc gttctaggtg ccctaacagc
     9301 tccggcaaat gatctgccgg agcacttccg attaccgcct caagctcgga tagcagattc
     9361 ttcatcgtac ctcaggccgt tcccacggcc ccgtgtgatg cattcatgct ctctgtgcgt
     9421 gccagaagtt cggccgcaaa cagatgccac agtttgaacg gatcgcttgg catcggtcgg
     9481 ttctggcttc gcaggtaggc gatcaacgat tctgctgaac acgttgccag aacattgagg
     9541 gcctcttgcg gcgtcagctc cgacgcccaa tgacgaagac gcatgatcaa cacatcgtcc
     9601 ggcacttctt gcgggagact ttggctatgc tgttcgcttg ccgcttccat gctggcctca
     9661 gtaccgcaaa tacggcgagg tgtaatagct ttgccacggg ccaggctgat tctgttgctg
     9721 actattctgc gcctggatct tggcgagttt ttcagcgatc ggcccccgca ccaatccatc
     9781 cattcccgcc gacatagccg gctgccccac gctttgcagc atgtcgccga tgtaattccc
     9841 gaccggcgcg accgcattca gcccttggaa ggcgtcatac ccaatttgcg gcgtggctga
     9901 gagtccaggg gtgtaggtcg gcagcgcact caccgcgaac cgctgattgc tcaccgccat
     9961 gtcggcattc ttctggttca cgtcccgaat catcttcgcc ttgttcgcgg ccagattttg
    10021 gccgagtacg cgtgtcgccc gatcgtagcg gccaggcgtg tagacgttgc ctccgccgga
    10081 gttcttgccg actgccgaat tggccgagat acgcgacaca tccgcgaggc cctgtccgta
    10141 gtcctcgttc acgtcgtctt tcatgccacg catcaagtcc tcgtcgtacc cgctcttccc
    10201 ttgcgagtag gtcatgagct tgtcgcggat cggactagtc gccccgagga acttctcgcg
    10261 ttcccggttc tggtagtcga tatacatctg agccgctttg cgcgcggcct tcgcttgcga
    10321 gtcaccacca aaggctttcg caatcccacc gccgatactc gccgcgcccc cgccgagcgc
    10381 catcatggtg aacggttcca tgcttacccc tccttgtccg ggtcgggttc aggcacatgc
    10441 gcgagatccg cgcggttgca gtactcgacg agcacatgac ggccgatgag atccggaaga
    10501 ctcgtgcaat tcttaattct gggatgaagc gacaccggcc ccgccggctc gaactttgag
    10561 cccaagagca tttccccgac cttctcaagc ggagcgccat attgcagcgc caacgaagcc
    10621 acacgcgcca gcacgtcata caacccgacc gtttccgcac tgcaatccgt acccttcact
    10681 cggagaaaga tttctcctgg tgaagcgtca tcgtggacgc tgatatagat cgtcctacga
    10741 ccgctaatgc gcgccttctg gataatgtgg ttccggcgtg agggtagttc tcggcgtggt
    10801 gtcgtcatgt cacgagttcc cccagcgact cgccaactca ggcagcgctt cggtatagcg
    10861 tccgttgata ctcgaccacc gcaacggaac taggccaatc ttccccacct cacggaagcg
    10921 caccttctgc acatagagat gtaccggctc gtcagggtcg ttctcgtcgc gccagactgc
    10981 caggcaattg tccgccttgt tccgccagtg agcagatccg gagatgtcat agggagttgg
    11041 aacgggatag ctcccatcgt ctcttcggta cagcttctgc gggtgagcga caagccacac
    11101 gtgcacgcca tagttgcggg caaatcggcg aatctgcgtg agggacgtgc tgatgtactc
    11161 ggtttcagtc gtcgtaccgc gcgcgtggtc gaactcgttc cagggatcga gaatgaggcc
    11221 gcggattcca tgccgtgcta ccaatgcctt ggcatgctgg agaagagccg gaatcgtgag
    11281 ggcttcatcc ggggcaataa acacgaaatg cccgtgcagc cagtccaggg ccatcacgag
    11341 ttcatggggg gtcatccgtc gcgtagggcc ttggcggaac ggccagccgg tatactgctc
    11401 gacgagcttg gcaatatgcc gagagaccgg caggttttcg ggtgagcaga cggcaatcat
    11461 ccactcgtgc tgtctggaaa gattcaccgt caacgcatcc aggaactgac ttttcccgtg
    11521 cgagggaatt cctgtgacga tggtcatttc accaggccga accgtgtaat gctgatccac
    11581 gctcggccat cccgtactga cgccgccagg taacccttcc tggtacagac tcatcacatc
    11641 accagcgagg tctgcgacct gcaacacacc ttcgagcgga tacggcttcg cctctgcgat
    11701 acatcggcga agctcttcag ctccgctctg aatcaacacc tcgttcgcat ctttgcagcc
    11761 ttccggccat cgaacacgaa aacagcgctc cgggcccagt cggcgggcca gttcagcttc
    11821 cagcgtgtgg cctggagcat cgttgtctac agcaaggacg atcttcttca gccggtccag
    11881 gtgtgcggcg cagttggtca gatatccaaa cttcgcgtca ctgggtttcg agttggccgg
    11941 cggggctcca tccggcacac tcacggcgtt acaaatccca gcgacctcga gcgccagctt
    12001 gtcacattcg ccttcgacaa tcagtgccca gtcttcggta agatcgtcca ggccgtacag
    12061 aactttctca gccccgccga cttgccggaa gtctttgcct tccagcgacc ggtatttgag
    12121 gttgacggcc tcgccttggc ggaaataagg gaagacgacg caaggggctt cgtcttcgag
    12181 ctgaggcagg taggcatgct gcagcgtgat gcagtgtcga gccacgacgg tttctgaaat
    12241 cccgcgcttc tcaaaccagt cacgcacagc cggcggaact gtggacggtt tttcgaagtg
    12301 tggcttgacg acgcgcttca acggccggga gggcgcatcg gcgccagatc gcaacgagcc
    12361 ggcccatccg caatggtgac agacccacac tccttcaacc gtgtttacac tcaagcagcg
    12421 gaccttggat ttctttcggg tgtggctaca ctgggggcag gtggtttgca cctccacgcc
    12481 ggttcgccca ccaatgtcaa tctggaaatc ctcgaaggtt ttcacagcgg aagtggctcc
    12541 tttcgcttca cagactggat atactcatca tcccaccgtg cttggtttag ccaggtcgct
    12601 ggcatgggaa tgaactttcc accttgctcc ctccacttcg tggtttgttt cgcttcggca
    12661 atcttggcca acatgcggtc gagtaatgaa tcgtccggcg tgtgcgccac ccaccaacgg
    12721 atacaagccc ccttaccttc tttcctcgga taggctgccc aaaacttccc aagacgtgtg
    12781 caactctcct ccggcaattt gtcacccttc ggactatata tattcttact gatggttctc
    12841 ttaagaggat tgggtggcgc cggcgccagg ggtcccatgt cacaaacgcc acctggcaat
    12901 atgccagggt gacaattcgc cggatggcaa ttcgccacct gatagaggtt ggttccgtgg
    12961 ggtccttgcc gctcactcag ggtcaattct ccggctgtta taagtcgccg aatcgagcgt
    13021 tgaacgttcc ggccgtcgag gcgtgatttc agtccaagcg tggcaatact gggaaacgct
    13081 gttcctgaat catctgcgaa atcggcgatc gccaagagta cgagcaaatc cccgcccttt
    13141 gcccgagact gcttccacac gtggctcatg acgcggatgc tcacagccgg actcctcccg
    13201 tgctctcggc aatttcaaga aaagcttcgt cactgattgg cactccctgc tcaccctcca
    13261 accgctgccg aagttcgacc cggcgcgcga tttccttcag tactccgccc aacacggagc
    13321 cgactggagt aaattcactc gcccccacag gctctgtctg tccaccgcgc gtcgcataaa
    13381 gcgagttatc agttgttcgg cgcatgagcc actgatccct cttgcgactt tcgttcacgg
    13441 aaaaccggtc gaaacaatgc agcaggagag gtgcccagga tttgggcgaa tcgcgcacac
    13501 tcctcagttg ttggcttgat aagccttcgc tccacgaggg aatagcgggc ttgcgacatt
    13561 ccagcctcac gccccatatc ccactgcgtc atccgccgat tgattcgcat aaattttatt
    13621 ttcagcatca caaccccctt tctcgttgag tgcaaaataa tgaaacacta ttcagccccg
    13681 tacactcaaa ggattatggg gaacaaatgc gtataacaat ttgtaacggc gcatggcagc
    13741 agaacgcaga atgccagctc cagcgttggt ttttcgctgt gcacgggtag gcgcacgagg
    13801 atgtggaact cttcgattta ggcgcggctg tttctgcgga gagctagttg cagtcgaggg
    13861 tat
//
LOCUS       DAORQN010000031        13461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_284_length_13461_cov_9.737879, whole genome shotgun sequence.
ACCESSION   DAORQN010000031 DAORQN010000000
VERSION     DAORQN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13461
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_13461_cov_9.737879"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>884
                     /locus_tag="PK614_03565"
     CDS             <1..>884
                     /locus_tag="PK614_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB81315.1"
                     /translation="AVYSLVDFVDRGGTWRDRYIRANAFGSDYNWLSTYMVMTIPVVG
                     SLIVLGRLVWFRAAQALALALMGGAQLFSYTRGGWLGHAAQGIAFATMVGGRRLALWV
                     LGLLAVAGAGLLVASQAGFQRDTVAASTVDTRLTVWAIGLRQVATHPVFGIGYGNNSF
                     VKKFPEYSSEAQAQVSEGTRIIPAMHNTFLMVALGSGLPALLSFVWIFVALLRRLIPI
                     PWRAAWNDRWSVLAAGIGLAVIGFAVRNLFDYMFMGSLAHIFWLLAAVGITVTGSAWR
                     GVGSGRQPGSPQDEGTSQ"
     assembly_gap    885..906
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1101..2108
                     /gene="gmd"
                     /locus_tag="PK614_03570"
     CDS             1101..2108
                     /gene="gmd"
                     /locus_tag="PK614_03570"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRB81316.1"
                     /translation="MNALITGITGQDGSYLAEFLLARGYDVYGIIRRSSSFNTARIDG
                     IYQDPHTSGAKLHLVYGDLNDASSLNKIIRTVQPDEIYNLGAQSHVRVSFDIPEYTAE
                     ITGLGTVRLLEAIRESGLRPKFYQASSSEMYGKVQEVPQRETTPFYPRSPYGAAKVYA
                     HWITVNYREAYGLFACNGILFNHESPRRGETFVTRKITKAAARIKLGLQQELFLGNLD
                     AKRDWGYAGDYVEAMWLMLQQDEPDDYVVATGETHTVREFLDVAFGQLGLDWQRYVKI
                     DPRYYRPTEVDLLIGNPAKAQQKLGWKPTVDFHRLAIMMVEADVEAERLKLQGTASKG
                     A"
     gene            2109..3059
                     /locus_tag="PK614_03575"
     CDS             2109..3059
                     /locus_tag="PK614_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRB81317.1"
                     /translation="MIDTHARIYVAGHRGMVGSAIVRALEARGCDNLLFRTSKELDLR
                     DNRRVAEFFAETKPDYVYLAAAKVGGILANSTFPAEFIYDNLAIQTNVIHHAYVHGVK
                     KLLLLGSSCIYPRDSPQPMKEEYLLTGPLEPTNEWYAVAKIAGIKMCQAYRRQYGCDF
                     IAAMPTNLYGPNDNFDLQTAHVLAALLRRFHEAREQGTPPTLWGTGAPRREFLHVDDC
                     ADACLFLMDRYSDARIMNVGAGQEISIGELATLVAEVVGYQGKIRWDPAKPDGMPRKL
                     MDCARIHALGWKSTIALREGLFETYQWYKRCAGPASAATP"
     gene            3110..3790
                     /locus_tag="PK614_03580"
     CDS             3110..3790
                     /locus_tag="PK614_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRB81318.1"
                     /translation="MKASVKAFVQARMSSQRYPGKVLAPFRGEAMIRHVLRAVEHTFS
                     SEDIVVVTSTELSDDPLVRYIESLGMHVHRGSLNNVLARFQSGAREYPCEWIMRVNGD
                     SPLLFPEVLRLAIPHVAVDAPDVVTTVFPRSFPKGQNVELIRVSALQTIRMEETTLND
                     QEHVTPFFYRQPDRFRIVNLDSGNRSLAEVGLAVDTIEDLSRLEQLSIDELRRFRPPV
                     LSASVASM"
     gene            3829..5004
                     /locus_tag="PK614_03585"
     CDS             3829..5004
                     /locus_tag="PK614_03585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81319.1"
                     /translation="MTDTLPIPFARPWITDEDRHAVLEVLSGHILTHGPQCKSFEQEF
                     AAFLGGGHCVSVSSCMAALHLAYLHFGIGPGDEVIVPAQTHAATAHAVEWVGATPVFA
                     DCESGTGNLTVSAIEAALTPKTKAISVVHFAGIPCDMPPIVDLAQRYNLRIVEDCAIA
                     LGARHRGRHVGLFGDAGCFSFYPVKHMTTGEGGMFVSRHEGVSQAVAKLRAFGVDRTH
                     SERTVPGMYDVPSLGLNYRMSEIQAALGRSQLRRMSVVLAQRASQFDRYRKLVGDLSN
                     VRLIDTDAAECRSSHYCASVVFEGENASKRNEMVERLNGAGIGTSIYYPQPVPRMTYY
                     RNKYGFVPGSYSQAAHISDHSVALPLGMHVADGDVERIVATLKCSLTESASGKGLTR"
     gene            5001..6065
                     /locus_tag="PK614_03590"
     CDS             5001..6065
                     /locus_tag="PK614_03590"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB81320.1"
                     /translation="MIELQGRKIALIGGAGFIGHNLALTLAEMGAEVHAVDSLQVNNL
                     GAFSNSHEDANKALYLHFIHDRLRLLQEAQIPLHVVDARDYHVLSRCLNDMKPDTIVQ
                     LAAIAHANRANKDPYSTFDHSLRTLENALDCARGLKARFIYFSSSMVYGNFDGEAVTE
                     ERHCEPLGIYGALKYGGEKLVIAYSQVFGLSYTIVRPSALYGQRCVSRRVGQAFIEGV
                     LRGAPLSVNGDGSDALDFTYIKDLLQGLVLCIGKDEARNQIFNLTYGGARSLAQMIDI
                     VRQQFPNVEVKFQPRDALMPERGTLSIEKAKRLLGYCPAYPLEKGFVDYIQWYKELAA
                     RHQKFFTPLQGPSVSGSNQR"
     gene            6062..7072
                     /locus_tag="PK614_03595"
     CDS             6062..7072
                     /locus_tag="PK614_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRB81321.1"
                     /translation="MKLFGKNLDETVAVVAEIGLNHEGDVAAAERLITLAAQSGADAV
                     KLQTYTPERYASASDPQRLARVTQFALSEEAHLQLATHAAKLDIPLFSTALTEDVVGF
                     LSRWCPAIKIASGDVTFEPVIRAAIRTGKPVILSTGNCTTDEIDQALKWCSLELDEIA
                     LNDRLILMHCVAAYPVPIEQANVSSVQFLRQRYGLTTGYSNHVIGAEAVLAAVAVGAQ
                     LIEVHFTDQREGREFRDHALSFEPHDLKGLMRSVERVKASLGRFGKEIQPCELPLRDV
                     IRKGVVAARALSAETVLSADDLMWARPATGVKAAEWPALIGKRLVRPLRRGEQITWNE
                     VR"
     gene            7075..7788
                     /locus_tag="PK614_03600"
     CDS             7075..7788
                     /locus_tag="PK614_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRB81322.1"
                     /translation="MRVLILTTDTLHHSYFVRELVSHVPKLHVILETTGVRPSFEVTH
                     PFEPVRDQYERDMWFGGNTPSVSDFAEAMTCPNVNDSEAVKAVQQFVPDITVAFGIRK
                     AEPALIRAAGPRVVNLHGGDPEFYRGLDSHLWAIYHNDFRNVVTTLHVLNEALDDGAL
                     IGIRPVALYRDMRLHQLRQSNTESALNLVRAALEELQRTGHIASRSQRQVGRYYSFMP
                     TVLKEVCVKRFEKYCARLE"
     gene            7785..8684
                     /locus_tag="PK614_03605"
     CDS             7785..8684
                     /locus_tag="PK614_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB81323.1"
                     /translation="MNYPSRISDHQIAIFLFHGVTLPVECAVRNYTRKHIERDYFATV
                     LRGLKRAGTPVSMNDILNHRELGSPLPPRPFAVTFDDGFRNNLSVAAPVLVDEHVPAT
                     FYISTDFVERNRMSWIDRIEFAVEQCIDAELQLPWGRRRFRGDEERRAFLSEIRHIVK
                     NDLRIDGEKLATDVQVQLGVAITYSSSHPLDQKLDWNDVRTLAAEPLFIVGGHGHSHV
                     ILDSLTDQDLECELDTGMRLLQEKAAIHGPHYSYPEGMPNCYSARVIDALKRRGVRCC
                     PSAVDGVNDSTADLFHLKRVMVV"
     gene            8736..9740
                     /locus_tag="PK614_03610"
     CDS             8736..9740
                     /locus_tag="PK614_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB81324.1"
                     /translation="MPKLRVGVIGLGVGAKHAEAIARIPECELAAVCDKNPTTLAAIA
                     GTFPGVTALERDTDLLDDPSIDLVCIASYDDCHFSQTLRALERGKHVFVEKPFVLHEV
                     EAIRVREQLSRRPDLRLSSNLILRRSPRFLDLRQRIQAGQFGDLYHLEAEYNYGRLEK
                     ITEGWRGKLPFYSVVHGGGVHVADLLMWLAHDRILEVSAVGNALASQGSGFGNFDNVV
                     AILRFASGAIGKLGVNFGCRLPHFHAMALYGTKATFLNGRDCAWLYTSADPHVPPLIL
                     NTAYPGVHKGGLLESFVAAIQGRGKAEVSEEDVFASLSVCFAIERSAHSGLPVKVDYV
                     "
     gene            9733..10500
                     /locus_tag="PK614_03615"
     CDS             9733..10500
                     /locus_tag="PK614_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB81325.1"
                     /translation="MSDRPSKVSGSHSVRGLSIVPDAWLATIMNRDVHRVTGVWEKDE
                     GNPVRQELRRLTLRPGFAFAKIATQEVRTSHQLEECGFRIVDTALTLETRRLSHVGTA
                     GSRVRLATSDDAATVVGIARQSFRYSRFHLDPDIPLSLADEIKARWAGNFFSGQRGDY
                     MVVAECDGRVVGFLQLIAAADHVLMIDLIAVHPDSRGLGLAAAMIGFAGKKACGHPRM
                     VRVGTQSANLPSLSLYHKLGFQVVSSSHIFHHHGPSR"
     gene            10567..12396
                     /gene="asnB"
                     /locus_tag="PK614_03620"
     CDS             10567..12396
                     /gene="asnB"
                     /locus_tag="PK614_03620"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB81326.1"
                     /translation="MCGIAGFIGTRHLSEETARACLALMRRRGPDASGVRRFRTSSGR
                     HVLLLHSRLSIIDLDSRSNQPFNVDRCWMALNGELYNYLEIKQRLTEVGISFRTRSDT
                     EVFLAAICRDGWGALDQAEGMWSLAVYDEDREQLTLSRDRFGEKPLYVVRSPEGLYFG
                     SEAKFLFALMERVLPVNRQHVRRYLVNGYKSLYKSGERFFEGLEEVPPAALLRIDADG
                     KETQSVYWEPAFRPNDSMTYGEAVAGVRARIIQSMELRLRGDVPAAFLMSGGVDSNVL
                     IGIAKRVFDYDVHGFTILTEDERYDEREMLDRSVAELGVRHTGLKADPIDYIDNMKLL
                     VRYHDAPVSTISHYVQWLLMREVHAKGYKICLSGVGADELVSGYYDHYLMHLAEIRDL
                     PEYSSTREAWEQYVRPLVRNPYLRDADLFVNAPQFRDHIFLNSDEFAGYLTHPFCEPF
                     VEHHYADSLLRNRMMNEMFVEATRVILHETDLNAMYFSIENRSPYLDRHLFEFCYSIP
                     TRYLSRNGYNKVVLRDAARGLAPDCVLNERRKVGFNAPIHAFLDVNDPAVMEWLLEDS
                     PVFDLLKRKKITELLRQPRLANSASKFLFYFVNAKVFLEEFAG"
     gene            12433..>13461
                     /locus_tag="PK614_03625"
     CDS             12433..>13461
                     /locus_tag="PK614_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRB81327.1"
                     /translation="MRYCVSCVLPDTRPNLSLGPDGVCNACRVHSSKTEIDWQSRARA
                     FERLVAEIKARSRGYDCVIPVSGGKDSTWQVIQCLSAGLTPLAVTWKTPSRTPIGQQN
                     LDNLVSLGVDHIDYQINPKVEKRFMLRALERFGSTAIPMHLALFNIPLRIAVKFGIQL
                     VVWGENSAFEYGSTNLAHTGMKLDDEWHRTYGVTHGTTASDWVGEDFTEKDLAGYAGP
                     HSNELEQSGVHAIFLGWYFPWDPQKSLAVARAQGFQSQPAGARTGYYDFADIDDDFIS
                     IHHWLKWYKFGFTRLFDNLSLEIRNRRLTREQAVAIIRARGDQTPHEDIQKFCRFVGI
                     EQAQFWKTC"
BASE COUNT         2803 a         3398 c         4057 g         3181 t
ORIGIN      
        1 tgccgtctat tcgttggttg attttgtcga caggggcggc acgtggagag accggtatat
       61 ccgggccaat gcgttcgggt cggattataa ctggctcagt acttacatgg tgatgacgat
      121 tcccgtggtc ggcagcctca tcgtcctggg tcgcctggtc tggtttcggg cggcacaggc
      181 cctggcgctt gcgttgatgg gaggcgcgca gcttttttcc tatacacgtg gcggatggct
      241 tggtcatgcg gctcaaggca tcgcatttgc gacgatggtg ggtggccggc gtctggcttt
      301 gtgggtgctg ggactcctcg cggtggcggg agcggggctg ctggtcgcgt cgcaggccgg
      361 atttcagcgc gacacggtgg ccgccagcac tgtggacacc aggttgacgg tgtgggccat
      421 cggattgcgg caggtggcga cgcaccccgt gttcgggatt ggatacggga ataattcgtt
      481 cgtgaaaaaa ttccctgaat actcatcgga ggcacaggca caggtgtctg aagggactcg
      541 catcattccg gccatgcaca acacctttct catggttgcg ctggggagcg gccttcccgc
      601 gttactcagc tttgtctgga tctttgtcgc tctcctgcgc cggctcattc cgattccctg
      661 gcgggctgca tggaacgata ggtggtcggt gctggctgcg ggtatcgggc tggccgtcat
      721 cggcttcgcc gtacgcaatc tcttcgacta tatgtttatg ggcagcctag cgcacatatt
      781 ttggctgctg gcggcggtcg gcatcaccgt gacggggtcg gcctggcgag gggtcggatc
      841 cggtcggcag ccggggagcc ctcaggatga gggcacctca cagcnnnnnn nnnnnnnnnn
      901 nnnnnnggca ccgaactgaa gatgcgggct gataggcgga gggtcggctc acgtctatgg
      961 tgtgggtgcg gacctgcact gttgcttgac accgcgtcgc agatctatca caagtggggt
     1021 cattgtggtg tgtcgcctgg gggatggata gtgatgtgcc cgttccttag ggcgggaaga
     1081 ccgatcggag tgcctgctca atgaatgcat tgatcaccgg tatcacgggc caggatggtt
     1141 cgtatttggc cgaatttctt ctggctaggg gatatgatgt gtatgggatc attcgccggt
     1201 ccagttcctt caacacggcg cggatcgacg gcatttatca ggatccgcat acctcgggag
     1261 ccaagctgca tctggtctat ggggatctca acgatgcgag ctcattgaat aaaatcatcc
     1321 gcaccgtgca acccgacgaa atctacaatc tcggggcgca gagtcatgtc agggtcagct
     1381 tcgatattcc cgaatatacc gcggaaatca cgggcctggg aacggttcga ttgttggaag
     1441 cgattcgtga gtcggggctg cgtcccaagt tttatcaggc ctcatcgagt gaaatgtacg
     1501 gcaaggtgca ggaagtgccg cagcgggaaa ccacgccctt ttacccccgt agtccctacg
     1561 gcgcggcgaa ggtctacgcc cactggatca cggtcaatta tcgtgaggct tatggcctgt
     1621 tcgcctgtaa cggcattctg ttcaatcatg aatcgccgcg ccgtggcgaa acattcgtga
     1681 cgcggaagat cacgaaggcg gcagcgcgga tcaaactggg actgcagcag gaattgtttc
     1741 tcggcaactt ggacgcgaaa cgggattggg gatatgccgg agattatgtc gaggcgatgt
     1801 ggctcatgct gcagcaggat gagccggacg actatgtcgt ggcgaccggt gaaacccaca
     1861 ccgtccgcga gtttctcgat gtggcgttcg gccagctggg gttggactgg cagcggtatg
     1921 tgaagatcga tccgcgatat taccgcccga ccgaggtcga tcttctgatc ggcaatccgg
     1981 cgaaagcgca gcagaagctc gggtggaaac cgacggtcga ttttcaccga ctggcgatca
     2041 tgatggtgga ggcggatgtg gaagccgaac ggctgaagtt gcagggcacc gcgtcgaagg
     2101 gggcctaggt gatcgacaca cacgcgcgca tttatgtggc cgggcatcgg gggatggtgg
     2161 gatcggccat cgtgcgggcg ctcgaggccc ggggatgtga caatctgctc ttccggacca
     2221 gcaaagaatt ggatttgcgt gacaataggc gcgttgccga attttttgca gagacgaagc
     2281 ccgactacgt gtatcttgcc gcggcaaagg tggggggtat tctggcgaac agcacgttcc
     2341 ctgccgaatt catctatgac aatctggcga ttcagaccaa tgtcatccat cacgcctatg
     2401 ttcacggcgt caagaaattg ctgctgctgg gctcatcttg tatctacccg agagattccc
     2461 ctcagccgat gaaggaggag tacctcctga ccggtccgct ggagccgacc aacgagtggt
     2521 atgccgtggc caagatcgca ggtatcaaga tgtgccaggc gtatcgccgg caatatggat
     2581 gcgatttcat tgcagccatg cctacgaacc tgtatggccc gaacgacaat ttcgatcttc
     2641 agacggcgca cgtcctggcg gcattgctgc ggcgcttcca tgaagcgcgt gagcagggca
     2701 cccctcccac tctgtggggc accggggctc cgcggcgtga gtttctgcac gtggacgact
     2761 gcgcggatgc ctgcctattc ctgatggatc gctattccga tgcgaggatc atgaatgtcg
     2821 gggcaggcca ggaaatttcg atcggcgaat tggcgacgct ggtcgccgag gtcgtcggat
     2881 accaggggaa gatccgctgg gaccctgcca agccggacgg catgccgcgg aagttgatgg
     2941 attgtgctcg aatacatgcc cttgggtgga aatcaaccat tgctctgcga gagggactgt
     3001 ttgaaacgta tcagtggtac aagcgatgtg cgggtccggc atcggctgcg actccatgat
     3061 gcaatgaatc cctctgcgat caacaccttt ttcggggcca ggtcttgaga tgaaagcgag
     3121 tgtcaaagca ttcgttcagg cgcgaatgtc ctctcagcgg tatccgggca aggtgctcgc
     3181 tccgtttcgt ggcgaagcga tgattcgtca tgtgctgcgt gcggttgagc atacattttc
     3241 ttctgaggac atcgttgtgg tgacgagtac cgagctgtcc gatgatccgc tcgtccggta
     3301 catcgaatcg ctgggcatgc acgtccatcg aggttccttg aataatgttc ttgcccgttt
     3361 ccagtccggt gcgcgggagt atccgtgtga gtggatcatg cgcgtcaacg gtgacagtcc
     3421 gctactgttt ccagaggtgc tgcgtcttgc gatccctcac gttgcagtgg acgctcctga
     3481 cgtggtgact accgttttcc ctcggtcgtt tccaaaggga caaaacgtcg aactcatcag
     3541 agtcagcgct cttcaaacga ttcgcatgga agagacgacg ctgaatgatc aagagcacgt
     3601 caccccattt ttttatagac agcctgaccg ctttcgaatt gtgaatctcg attccgggaa
     3661 tcgaagcctc gccgaagtcg gtttggccgt cgatacgatc gaggatttgt ctcggctcga
     3721 acagttatcg atcgacgaac ttcgccgttt ccgtccgccc gtgttgtcag ccagtgttgc
     3781 gtcaatgtga ctctacatga gaatttaaac cagaaggaca ggatacacat gactgacact
     3841 ctgcccatcc cctttgcccg gccttggatc acagacgagg atcgacatgc ggttctggag
     3901 gtgctcagtg ggcatatctt aactcatggg cctcagtgca aatcgtttga acaagagttt
     3961 gcagcgtttc tgggaggagg ccactgtgtg tccgtcagtt cgtgcatggc ggcattgcac
     4021 ctcgcgtatc ttcatttcgg gattggcccg ggtgatgagg tgatcgtgcc tgcacagacc
     4081 catgcggcga cggcccatgc cgtggagtgg gtcggagcca cgccggtatt tgccgactgt
     4141 gagtcgggca cagggaattt gaccgtgtcg gcgatcgaag ctgcgctgac tcccaagaca
     4201 aaagcgattt ctgtcgtgca ctttgccggc attccatgcg atatgccgcc cattgtcgat
     4261 cttgcgcaac ggtataatct caggatcgtt gaggactgtg cgattgcctt gggagcccgc
     4321 caccgggggc gccacgtcgg actgttcggc gatgccggct gtttttcctt ctatccggtg
     4381 aagcatatga ccacgggtga aggtggaatg ttcgtcagtc gtcatgaagg cgtctcccaa
     4441 gctgttgcta agctgcgagc ctttggcgtg gatcggactc acagtgagcg gaccgtgccg
     4501 ggcatgtatg acgtcccaag tctcggactg aactaccgga tgagcgaaat tcaagcggct
     4561 ctcggccgct ctcagcttcg gcgaatgagt gtcgtgttgg ctcagcgcgc ctcacagttc
     4621 gatcgctatc ggaaactggt gggcgatctc tcgaatgttc ggctcatcga tacggacgct
     4681 gcggagtgcc gatcaagcca ctattgcgcc agtgtcgtat tcgaagggga aaatgcgagc
     4741 aaacgaaacg agatggtcga acgactgaat ggggccggaa tcggaaccag catttactat
     4801 ccgcagccgg ttccacggat gacctactat cgaaataagt atgggtttgt gcctggtagt
     4861 tattcgcaag cggcacacat tagcgaccat tcggtcgcgc tgccgctagg aatgcatgtg
     4921 gcagatggcg atgtcgagag aatcgtggct accctgaagt gtagcttaac cgagagcgcc
     4981 agcgggaaag gactcacacg gtgatcgaac tccaaggacg gaagatcgcg cttatcggag
     5041 gcgctggttt tattggccat aatcttgcct tgaccctggc agaaatgggt gccgaggttc
     5101 acgccgtaga cagcctgcag gtcaacaatc ttggtgcgtt ttcgaactcg catgaggacg
     5161 cgaataaggc gctgtacctt catttcatac acgaccgctt gcggttacta caggaggctc
     5221 aaattcccct tcatgtcgtc gatgcgcggg actatcatgt gctgtctcga tgcctcaacg
     5281 atatgaagcc tgatacgatt gtgcaattgg cagccatcgc ccatgcgaat cgtgccaaca
     5341 aggatccata cagcacgttc gaccatagtt taagaacctt agagaatgcg ttggattgcg
     5401 cgcgggggtt gaaggcccgc ttcatctact tctcatcttc gatggtctac gggaatttcg
     5461 acggtgaagc tgtgacggag gaacgtcatt gcgaaccgct gggtatctat ggcgcgttga
     5521 agtatggagg agaaaaactc gttatcgcgt atagccaagt ctttggtctg tcctacacga
     5581 ttgtcagacc ttcggccttg tatggccaac gctgcgtcag tcggcgtgtc ggacaggcgt
     5641 ttatcgaagg cgtccttcgc ggggcgcctc tcagcgtgaa cggcgacggc agcgatgcgc
     5701 tcgatttcac gtatatcaaa gaccttctgc aggggctggt gctgtgtatc ggcaaggacg
     5761 aagcccggaa ccaaattttt aatttgacgt atggaggagc gcgttccctc gcccaaatga
     5821 tcgacattgt gcggcaacaa ttccccaacg tcgaagtgaa attccaaccg cgcgatgcct
     5881 tgatgcccga acgcgggacc ctctctattg agaaggccaa acgattgctc gggtactgcc
     5941 ctgcgtatcc gctggaaaaa ggcttcgtcg attatattca atggtataag gagctggccg
     6001 cccggcatca gaaattcttc actccacttc agggtccatc agtgtcaggg agcaaccagc
     6061 gttgaaatta tttgggaaaa atcttgatga gacagtcgcg gttgtcgcgg aaatcggtct
     6121 caatcacgaa ggggatgttg ccgctgccga acgcctgatc actcttgccg cacagtccgg
     6181 cgccgatgcc gtaaagctac agacctacac tcctgaacgg tacgcatccg catcagatcc
     6241 acagagactg gctagggtga cacagttcgc tttgtcggag gaggctcatc ttcaactggc
     6301 aacccatgct gccaaactgg atattccact gttttcaacc gcgttgacgg aagatgtcgt
     6361 cggcttcttg agtcgatggt gtccggccat caagattgcc agcggagatg tgacctttga
     6421 gccggtcatc cgcgcagcga ttcgtacggg aaagcctgtc attctttcta ccggaaactg
     6481 taccaccgat gagattgacc aagcattgaa gtggtgcagt ctggagttag acgagattgc
     6541 gctgaacgat cgactgatac tgatgcattg tgtcgccgcc tatccggttc ctattgagca
     6601 agcgaacgtt tcgagtgtgc agtttctcag gcagcgatat ggactgacga ccgggtattc
     6661 caatcacgtg atcggcgcgg aggcagtact cgcggcggtt gcggtgggcg cacaactcat
     6721 tgaggtacat ttcacggacc agcgagaagg tcgagagttt cgtgatcatg cgctctcctt
     6781 tgaaccacac gacctgaagg gcttgatgcg aagtgtggaa agagtgaagg ccagcttggg
     6841 ccgttttgga aaggagatac agccctgcga acttccgttg cgggacgtca tacgaaaagg
     6901 agtcgttgcg gcaagagcgc tgtccgcaga gacggttctg tccgcagacg acttgatgtg
     6961 ggcgcgtcct gcaacaggag tcaaagctgc tgagtggcca gcccttatcg gaaaacgcct
     7021 tgtgcgtccc ctccgccgtg gagagcagat cacttggaat gaggtgcgct gaccatgcgc
     7081 gtgcttatcc tgacgacgga caccttgcac cattcgtatt tcgtgcggga gctcgtttcg
     7141 catgtgccca agttgcacgt catccttgaa accaccggcg tccggccgtc ctttgaggtg
     7201 acccacccgt ttgaaccggt acgcgatcaa tacgaacgtg atatgtggtt tggcgggaat
     7261 acaccatcgg tgtcggattt cgcagaagca atgacatgcc cgaatgtgaa cgactccgag
     7321 gcagtgaaag ctgtacagca gtttgtgccc gatatcacgg tcgcattcgg tattcgcaag
     7381 gctgagccgg cgctgatcag agccgccggg cctcgagtgg tcaatctgca cggcggagat
     7441 cctgagtttt atcgtgggct ggacagtcat ctctgggcca tttatcacaa cgattttaga
     7501 aatgtggtga cgactctgca tgtgctgaat gaagcgcttg acgatggagc cctcattggc
     7561 atacgtcctg tcgcgcttta tcgagacatg cgtctccatc aattgcggca aagcaatacc
     7621 gaatcggcgt tgaacctcgt ccgcgcggct ttggaggagt tgcagcgtac gggtcatatc
     7681 gcgagtcgtt cacagcggca ggtgggacga tactactctt tcatgccgac cgtgttgaaa
     7741 gaggtgtgcg tgaaacgatt tgaaaagtat tgcgctcgat tagaatgaac tacccgtcaa
     7801 gaatctccga ccaccagatt gccatttttt tgtttcacgg tgtgacgttg cccgtcgagt
     7861 gtgccgtccg caactatacc aggaagcata ttgaacgcga ctattttgcg accgtgcttc
     7921 gtggattgaa gcgggcgggc actccggtga gcatgaacga catactcaat caccgggagc
     7981 tagggagtcc tctccctcca cggccctttg cggtgacgtt cgatgatgga tttcggaaca
     8041 atctctcggt agcggctccc gtgcttgtgg acgagcatgt gcccgccacc ttttacatca
     8101 gcacggattt tgtcgagcgg aacaggatgt cctggattga tcgaattgag tttgccgtgg
     8161 aacagtgcat tgatgcggag ctccagcttc cctgggggcg gcgacggttt cgtggggatg
     8221 aagagcgccg agcattcctg agtgagatcc ggcatatcgt gaagaacgac ttgaggattg
     8281 atggagagaa gctggcaacc gatgtacagg tgcagttagg tgtcgctatc acctactcaa
     8341 gttcacaccc tcttgaccag aagctggact ggaacgacgt tcgaacactt gcggctgagc
     8401 cgttgtttat cgtgggagga cacgggcact cccacgtcat cctcgactct cttactgatc
     8461 aggatctgga atgtgagctc gatacgggta tgcgactgtt gcaagagaag gcggccattc
     8521 acgggccgca ttactcgtac cccgaaggca tgcccaattg ttattccgcg cgagtcatcg
     8581 acgcgttaaa gcgcaggggt gttcgctgct gccctagtgc agttgatggc gtaaatgatt
     8641 caactgctga cctgtttcat ctgaagcggg tgatggttgt ctgattatcc cctgcgtgag
     8701 tccgagacgt gcgtttcatg gacagcgatg tattcatgcc caagttgaga gtcggagtca
     8761 tcggtcttgg agtcggggcg aagcacgcgg aggccatcgc caggattcca gagtgcgaac
     8821 tggctgctgt gtgtgacaag aatccgacga cgcttgccgc catagccggt acgtttccag
     8881 gtgtgacggc gctcgaacgc gacacggatc ttctcgatga tccctccatc gacctggtgt
     8941 gtattgccag ctacgatgac tgccattttt cccagaccct acgggcgctg gaacgcggca
     9001 agcatgtgtt tgtggagaaa cctttcgtgc tgcatgaagt ggaggccata cgcgtcagag
     9061 agcaattatc tcgtcgccca gaccttcggt tgtcgtcaaa tttgattttg cgacgctcgc
     9121 cgcggttcct cgatctgcga cagcgaatcc aggcgggtca gttcggcgat ctctaccacc
     9181 tggaagccga atataattac gggcgacttg aaaaaattac cgagggatgg cgaggaaaac
     9241 tcccgtttta ttccgttgtg catggagggg gggtccatgt ggcggatctt ctgatgtggt
     9301 tggcgcacga tcgaattctc gaggtatcgg cagtaggaaa tgcgctggcg agccaagggt
     9361 ccggattcgg aaactttgac aatgtggttg cgatcttgcg ttttgccagc ggcgcaatcg
     9421 ggaaactcgg cgtcaatttc ggttgccgat tgccccattt ccacgcaatg gccttgtatg
     9481 ggacgaaggc gacgtttctc aacgggagag actgcgcgtg gttatatacg tctgcggacc
     9541 ctcatgttcc gccgctcatt ttgaataccg cgtacccagg agtgcataag ggtgggttgc
     9601 tcgagagttt tgtggcggca attcaagggc gagggaaggc ggaggtgagt gaagaagatg
     9661 tttttgcgtc tctatccgtg tgcttcgcga tcgaacggag cgctcattcc ggccttccag
     9721 tcaaagtaga ttatgtctga ccgtccctcg aaagtgtccg gatcacattc cgtgcgtggc
     9781 ctgtcaatcg ttccggatgc ctggctggct acgattatga accgtgatgt tcaccgcgta
     9841 accggggtgt gggaaaaaga cgagggaaac cctgtgcggc aagagcttcg ccggcttacc
     9901 ctccggcctg gttttgcctt tgctaaaatt gcgacacaag aagtgcgcac aagtcatcaa
     9961 ctggaagaat gcggcttccg aatcgtggat accgctctga cactggagac gcgacgcttg
    10021 tctcatgttg ggacagccgg ctctcgggtg cggcttgcga catcggacga tgcggcgacg
    10081 gtcgtgggta tcgcgaggca gagctttcgc tattcgcggt ttcatttaga tcccgacatt
    10141 cccctgagct tggctgacga aatcaaggcc cggtgggcag gcaatttctt cagcgggcaa
    10201 aggggcgact acatggttgt tgccgaatgc gatggtcgag tcgtgggatt tcttcagttg
    10261 atagccgctg ctgatcatgt cctcatgatt gatctgatcg ccgtccatcc agactcacgg
    10321 ggattgggat tggctgcagc gatgatcggg ttcgcgggca agaaggcctg tggtcatccc
    10381 agaatggttc gcgtcggcac ccagagcgcg aaccttcctt ctctttctct gtaccataaa
    10441 cttgggtttc aggttgtttc gagcagccat atttttcacc accacgggcc gtcgcgttag
    10501 gctgctgcac gcactgtttg cacagactct gctgcaggcg ccccgcctca atgaggaatc
    10561 gctgctatgt gcgggatagc tggtttcatt gggacacgac atctctccga ggagaccgca
    10621 agagcctgcc tcgctctgat gcggcgccga gggccagacg cttccggggt gcgtcgcttt
    10681 aggacctctt ccgggcggca tgtgttgttg ctgcacagtc ggctctcgat tatcgatctg
    10741 gattcgagat ccaaccaacc cttcaacgtt gaccgatgct ggatggcgct caatggagaa
    10801 ttgtacaact atcttgagat caaacagcgt cttacggagg ttggcatttc atttcgcacc
    10861 cgatccgata ccgaagtgtt cttggccgct atttgtcgtg acggatgggg tgcattggat
    10921 caagcagaag ggatgtggtc actagcggtc tacgacgaag accgggaaca gttgacgctg
    10981 tcacgtgacc gattcgggga aaaaccactg tatgtcgtga ggtcaccgga aggattgtat
    11041 ttcggatccg aagccaagtt cctgtttgcc ctcatggaaa gggttttgcc ggtcaaccga
    11101 caacatgtgc gccgttacct ggtgaatgga tacaagtcgc tgtataagtc gggggagaga
    11161 tttttcgagg ggcttgagga agtgccgcct gcggcgctgc tgcgaataga tgccgatggc
    11221 aaggaaacgc agagcgtcta ttgggagcct gcctttcgcc cgaacgacag tatgacctat
    11281 ggggaggctg tggcaggcgt gcgggcgagg attattcagt cgatggaact gcggttgcgg
    11341 ggtgacgtgc cggccgcatt tttgatgagt ggcggggtgg actccaacgt gctcatcgga
    11401 atcgcgaagc gtgtctttga ttacgacgtg cacggattta cgattcttac ggaagatgaa
    11461 cgatatgacg agcgcgagat gctcgatcgg tcagtcgctg aattgggagt gcgacataca
    11521 ggactgaaag ccgaccctat cgactacata gacaatatga agctcctggt gagatatcat
    11581 gatgcgccgg tttcaaccat cagtcattat gtgcaatggc ttctgatgcg cgaagtgcac
    11641 gcgaaagggt acaagatctg tctaagcggt gtcggtgcgg atgagttggt gagcggctac
    11701 tatgatcact acttgatgca tctggctgaa attcgagatt tgcctgaata ttcatccacg
    11761 cgcgaggcgt gggaacagta tgtccgaccg ttggtgagaa atccgtattt aagggacgca
    11821 gacttgtttg tcaacgcccc tcagtttcga gatcatattt tcttgaatag cgacgaattc
    11881 gccggttatc tcacgcaccc gttctgcgag ccgttcgtgg aacatcacta tgccgactca
    11941 ttgcttcgaa atcgcatgat gaatgaaatg ttcgtggagg cgacccgggt catccttcat
    12001 gaaaccgacc tcaacgccat gtatttttcc attgaaaatc gatcgcctta tttggaccgc
    12061 catttgttcg agttttgcta ctcgatcccg actcggtatc tgagtcgaaa cgggtacaac
    12121 aaggtcgtgt tgcgtgatgc ggcgcggggg cttgcgccgg actgcgtctt gaatgaacgg
    12181 cgaaaagtgg ggttcaatgc gcccattcat gcgtttcttg atgtgaacga tcccgcagtg
    12241 atggagtggc tgctggagga tagcccggtg tttgatctgc tcaagcgaaa gaagattacg
    12301 gaattgctgc gtcagcctcg attggccaat agcgcaagca aattcttgtt ctattttgtc
    12361 aatgcaaagg tgtttcttga agagtttgcc ggctgatggt cgcccggcgt cctgagaacg
    12421 gtgctggtta ttatgcgcta ttgcgtgtca tgtgtgttgc ctgatactcg ccccaatctt
    12481 tctctgggcc cggatggggt gtgtaatgcc tgtcgtgttc atagcagcaa aacagagatt
    12541 gattggcaaa gccgagctcg tgccttcgag cgtctggtgg cagagatcaa ggctcgaagt
    12601 cgcggatacg actgcgtgat ccctgtcagc ggagggaagg atagtacgtg gcaggtgatt
    12661 cagtgtctca gcgcgggact gacgccgctt gccgtgacct ggaaaacgcc ctctcgcacc
    12721 ccgatcggtc agcagaatct tgataatctg gtctctcttg gcgtagatca catcgattat
    12781 cagattaacc caaaggtcga aaagcggttc atgttgcgcg cgctcgaacg attcggaagt
    12841 acggcgattc ccatgcattt ggcattgttc aacattcctc tccgcattgc cgtgaagttc
    12901 ggtattcagt tggtggtgtg gggagaaaac tcggccttcg aatatgggag tacgaacctc
    12961 gctcatacgg ggatgaaatt ggatgatgag tggcatcgca cgtatggggt gactcacgga
    13021 accacagcct ccgactgggt aggcgaagac ttcacggaaa aagacttggc gggatacgcg
    13081 ggacctcatt cgaatgagtt ggaacagagc ggagtgcacg cgatttttct cggatggtac
    13141 tttccatggg atccgcagaa aagtcttgcc gtcgcacgcg cgcagggatt tcagtctcaa
    13201 cctgcagggg ctcgaaccgg ctactatgat tttgccgaca tcgacgacga cttcatctcg
    13261 atccatcact ggttgaagtg gtataagttt ggtttcactc gcctcttcga caatctttcg
    13321 ttagagattc gaaacaggcg tcttacgcgc gagcaagcgg tagctattat ccgagcgcgt
    13381 ggagatcaga cgccccatga ggatatccaa aagttttgtc gtttcgtcgg gattgagcag
    13441 gcacaattct ggaaaacatg t
//
LOCUS       DAORQN010000032        13306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_287_length_13306_cov_13.508641, whole genome shotgun sequence.
ACCESSION   DAORQN010000032 DAORQN010000000
VERSION     DAORQN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_287_length_13306_cov_13.508641"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1714
                     /locus_tag="PK614_03630"
     CDS             194..1714
                     /locus_tag="PK614_03630"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRB81328.1"
                     /translation="MKKIQVHAQVGRAENEAAEVLVLLHCEGGSDLAQEAAAIDAQLG
                     GQLAALIQRGEFEGKLGEGLLVHTQGKARAKRLLLAGLGKEKDLRVDAFRQALGAAVK
                     RVRQTKVTSFSVVLPAAMLEEIPVQDVAQALAEGAILGNYQFTAYRSANGSKPIDVER
                     LTIYTSQASLLPQMTEGIRRGVATAEATVLVRDLCNHPANVMTPTRIVREAKAVAKES
                     GVRLKVLEQKDMEQLGMGALLGVARGSHEPPKFIILEYKGAKAKKGDPPVVLVGKTIT
                     FDTGGISLKPAENMEHMKADMTGGAEVLATMRAAARLKLPLHLVSILPVAENMPGGRA
                     MRPGDIVTTLSGKTVEVQNTDAEGRLILSDALAYATRYKPAVLIDIATLTGACVVALG
                     QFAIGMFGNNDQLKEQVRNAGQRAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGM
                     ITAALFLSKFVGDCPWIHLDIASTDWSERERAYLPKGPTGIGTRLLIQFLLDRTLP"
     gene            1751..2902
                     /gene="nagZ"
                     /locus_tag="PK614_03635"
     CDS             1751..2902
                     /gene="nagZ"
                     /locus_tag="PK614_03635"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRB81329.1"
                     /translation="MTLREQIGQLFMMGFTGTTVTKDLASFLTAYKPGGVIFFRRNLE
                     SVQQIVDLTNGLQKLSPVRPLLIAIDQEGGRVSRLPAEFTIFPPCGQLGQCNSSELAY
                     SAAATIAKELRAVGINMNMAPVLDVNSNPDNPVIGDRAFGAAPELVGEMGSATIGGLQ
                     DNMVVACGKHFPGHGDTATDSHKELPVVDAGLQRLRDTEFPPFQQAIRYGVASLMTAH
                     VLYRALDPEAPATLSSAVIQRLLREEFRYEGVVFTDDLEMHAIIDHDGIGEAAVRSFV
                     AGCDVLLICKDQDRVMTAMQAMERAVKDGRITQERLDQSLARVAKLKARYLHPYKPVT
                     ISDARLVVGCRSHKVLLDSWHKTYARIPKPTSSDTAQAAPAHDSPVTHV"
     gene            3125..3511
                     /locus_tag="PK614_03640"
     CDS             3125..3511
                     /locus_tag="PK614_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81330.1"
                     /translation="MAIKKGDILDERKRFPDSCGLVVKVAGGGEGFQKILCCGHELTE
                     EDVVSDIISSRGRKKGALLPGAMLEEKRQFPDSCGLRLMVIDGGAGFQGIDCCGHTIT
                     AKAVNGLKFGQPLEDSPMPTGQAGTA"
     gene            3508..4032
                     /locus_tag="PK614_03645"
     CDS             3508..4032
                     /locus_tag="PK614_03645"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRB81331.1"
                     /translation="MTPPVTPPGPLEKSRRATMQWLLGFMDHLDRLGYVAAGFSLLAL
                     GMLIFVHAWYAFFLRSNDVPLLPAGLKLLNDLLLVIILLELFRTVVRFLQTEVLELEP
                     YLAVGIIACTRRVLTASAELSHQLEVVTREARQELFQQYVMDVGLNVTVIIVLILGVY
                     LLRMRPTRERPVTT"
     gene            complement(4371..4586)
                     /locus_tag="PK614_03650"
     CDS             complement(4371..4586)
                     /locus_tag="PK614_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81332.1"
                     /translation="MTGKTRTTLRLEQPGRLSEVLLQPGPQAFWQPPQVEEMLAPESR
                     YLLPQERAEQPQVWEEQGASAEQAAPV"
     gene            complement(4610..6055)
                     /locus_tag="PK614_03655"
     CDS             complement(4610..6055)
                     /locus_tag="PK614_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRB81333.1"
                     /translation="MGNPLFRTKSIERILADSDAPEHRLKRTLTAWDLTGLGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIMLSFILSGITCALAALCYAEFAAMIPVAGSAYTY
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNNILKLCGLELPYWATHPPGAD
                     GGIANIPAAIIVLLVTGILIVGVKESARATCGIVLIKLAVIAFFIAVGTSSVDTANWS
                     PFMPFGFAGVGAAAAIVFFAYIGFDAVSTTAEEAKNPQRDLPIGIFASLAICTVLYIS
                     VAAILTGLVPYSQIDVHAPVAEGLRMVGFKWGAAIVATGAVAGITSVLVVMMLGQIRV
                     FFAMSRDGLLGPWLSGVHPKFRTPHHATYLTGVAVAIMAALIPIGEAADMTNIGTLFA
                     FVLVCIGIMVLRYTKPNHPRPFRMPFMPVVPILGVLACLGLMYFLPWMTWIRFFVWTI
                     IGILVYTMYGMRHSKLATRPSTEQSETPRPA"
     gene            complement(6119..6895)
                     /locus_tag="PK614_03660"
     CDS             complement(6119..6895)
                     /locus_tag="PK614_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRB81334.1"
                     /translation="MKPVIGVTPDFNAGDRQEWGGKEPTYFLRARYVRAIEELGGVPV
                     ILPLVANQSARRRLLEGVDGLLLTGSGPDLDPTLYGEKKRYTFPVVAKRRSSFELDLV
                     RLAIRNHIPTLAICGGMQSMNVACGGSLYQDIPAQVDHVLQHRQTTPAVRLSHSISIT
                     PGSLLNRIVKRARMQVNSSHHQSVKAVGHALIASATAPDGIVEAIELPTHPFFLGLQW
                     HPEFLFERHLLHRRLFQTFLRAASRRPSLQPSPARRRNNS"
     gene            complement(6897..7370)
                     /locus_tag="PK614_03665"
     CDS             complement(6897..7370)
                     /locus_tag="PK614_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81335.1"
                     /translation="MNVSVYSPLKRRRSLWAQVLVFSLVTVCSLALLIAPVSAQESGG
                     ASNVPVSSNDASPSGVGIQAGSALATIVYFPLKLAFAIGGGVVGGLAYGFSGGSEQTA
                     KNIWIPSMYGTYIITPDHLKGDKPIRFLGVSGEPAREAGDYTPQEPAPLPLEPIR"
     gene            complement(7504..7962)
                     /locus_tag="PK614_03670"
     CDS             complement(7504..7962)
                     /locus_tag="PK614_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81336.1"
                     /translation="MAIVPSSDPAQYRVTFFFGPEAVEERPDHLRCVFNVKKRSWKGG
                     VQVAVDVAGRHLDRARERIGYASWLAELIHSDHQEEREELTSRADDLFVQSLCTSALN
                     LALQAGIEQVNQQIDAATFADELHQLLDEQPEQVTDRIRLELDLGETSEI"
     gene            8045..9274
                     /locus_tag="PK614_03675"
     CDS             8045..9274
                     /locus_tag="PK614_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRB81337.1"
                     /translation="MMASMNVADRSHARVIRIQQAIREQPGLDGWLFYDFRHLDPIAY
                     RVLLLDPSLHVTRRWYYWVPAQGTPVKLQHRIEPHVLDGLPGDAHAYVSWRDQQAALG
                     SLLHSAKRIAMQYSPMNAIPYLSRVDAGTIDLVRSLGAEVVTSADLVQQFEAVWDDAQ
                     LASHQVAAEGLRAIVDEAFGFVGTSLAARSSLTEYGLQQYILSRMQARGLVTSSPPIA
                     AVNAHSADPHYAPASEGSAPIRQGDLVLIDLWAKQPGPGAVYADITWTGFVGATVPAR
                     HQDIFQIVRRARDAAVTFVQGRVRAGECPYGWEVDDVCRRVIQDAGYGQYFVHRTGHS
                     IGEEVHGNGANIDNLETQDARRLLPGTCFSIEPGIYLPDEFGIRSELDVYLSARGAVV
                     YGQPVQADLLPIPIPAS"
     gene            9421..10275
                     /gene="tatA"
                     /locus_tag="PK614_03680"
     CDS             9421..10275
                     /gene="tatA"
                     /locus_tag="PK614_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB81338.1"
                     /translation="MFGTMGFSELIIILVIVLIIFGAGKLPQIGEGVGKALKGFKKEV
                     NDIPPPENATEQNEIAATPAQIQAQPAADIPQAAQPATVAPAASAPKATSPYTPGPEL
                     TPGTTAALMAAAGPQGPQAIQPAKPRVATVTPGQAAAGSAVAQSHQPPTMEDRMAAPA
                     PVMRAQYPPLPPGAQSKPVAKRPSAIVNKDAVARVQAAQAAMRTKAAQAQPSGLSVQD
                     MQGLGEGLGDAVRTFRQAVADVRSSVDPEMRTIRAEMDSAQKELEQSIEAAKQAPVLD
                     DDAPAKSV"
     gene            complement(10282..10497)
                     /locus_tag="PK614_03685"
     CDS             complement(10282..10497)
                     /locus_tag="PK614_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81339.1"
                     /translation="MKEFNGIETSILAGRKEPKPARNYEIGMQVRDEALGWIAQDTNQ
                     QDASKKEPVHFLIGRGPGITRAGEKIY"
     gene            10520..11311
                     /locus_tag="PK614_03690"
     CDS             10520..11311
                     /locus_tag="PK614_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81340.1"
                     /translation="MRSYFRLQFLFWLLLCSVVYGCYVDTPSGTPETVSGRLVELLAD
                     PDSAVRRTAAEALGKVGHKSSNASLIVALNDPDARVREAAALALGRLGDGKSGIALAG
                     HLADSAEPVRMASALALGEIEFSADREAQTLAVLRHPQGSARIAATRALLSLDTVSLS
                     ADLIKALRDPDARVRQGVAAVLGESGNPGAVSHLRSLLRTDGAASVRAEAAFRLGKIG
                     DNGVLAELSRAAEADPDMMVRGWARWAVQQITLSREFGSERQPSQ"
     gene            complement(11265..11783)
                     /locus_tag="PK614_03695"
     CDS             complement(11265..11783)
                     /locus_tag="PK614_03695"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB81341.1"
                     /translation="MKFPVTATREHQGKTLSIDCDQETLSLFDDTGQRLSILTWGTLI
                     EHMLSVDEDARFAHSRAHPRAPLAIKVHCTTGDGKHFDSLTGGISGGGLFIESSAPLA
                     PGTELKVEFALPDRPSQKFETRAKVAWARSKPERYLLFPGMGIQFTDIDPEAQARLID
                     LVASLNRTRATA"
     gene            complement(11849..12373)
                     /locus_tag="PK614_03700"
     CDS             complement(11849..12373)
                     /locus_tag="PK614_03700"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB81342.1"
                     /translation="MKFPVVSSNRHKGKTLEIDSSKEVITLVGINGEPMGSLTWDFLV
                     DQILTYRKPQQSREARSEPRISLSIRVKYHTPEGTQFESRAGGIGGGGLFIESFTPLA
                     VGTKLAMEFTLPESPGEWLSAKGVIAWVCPKADQYTFSPGMGVRFSDIAAETRNRVLS
                     LVHSVKSITQDEPK"
     gene            complement(12592..>13306)
                     /gene="clpX"
                     /locus_tag="PK614_03705"
     CDS             complement(12592..>13306)
                     /gene="clpX"
                     /locus_tag="PK614_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRB81343.1"
                     /translation="DEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVANVPPQGG
                     RKHPHQEFIQVNTSNILFVCGGAFVGLEHIIEQRMNRKSMGFGAEVRGRNDIRLGELL
                     PHVQPEDLLKYGLIPEFIGRLPVVATLDELDEQALIRILTEPRNALTKQYEKLLSFEK
                     VKLRFTEGALSAVARKAFTQKTGARGLRSILEEAMLDVMYDAPSQKQIKEVVITEDAI
                     SGKHAPIRIFEQDKDVKSA"
BASE COUNT         2581 a         3660 c         4191 g         2874 t
ORIGIN      
        1 cgaagtggag gtgcggcatg gggcggagtg gatactggcc gataagctgc cggtgcggtt
       61 ccagctccaa atgcacaaat acatttgggc gccggatatg cgtggcgttt gagaggggcg
      121 tgattcttaa agacggggcg gtctgtgaac ggctgatgat gaacgctgaa cggcaagaac
      181 caggggagga ttgatgaaga agatccaagt tcacgcacag gtcggacggg cagaaaacga
      241 ggcggccgag gttctggtat tgctccattg cgagggtggc tccgatctcg cgcaagaggc
      301 tgccgccatc gatgctcaac tcgggggaca gcttgccgct ttgattcagc gcggagaatt
      361 cgaagggaag ctcggcgaag ggttgctggt ccatactcag ggcaaggccc gggcaaaacg
      421 gctgttgttg gctggattgg gtaaggaaaa agatctacgt gtggatgcgt tccgccaggc
      481 gctcggtgct gcggttaaac gtgtccgtca gaccaaagtg acctcattct ccgtggtgtt
      541 gccggccgct atgctggagg agattcccgt ccaggatgtg gcgcaagcct tggcggaggg
      601 tgccattctc ggcaactatc aattcaccgc ctatcgcagt gccaacggca gcaagccgat
      661 cgacgtcgaa cggctgacga tctatacgtc gcaagcctcg ctgctcccgc agatgacaga
      721 aggaatccgg cgcggcgtgg cgactgctga agcgacggtg ctggttcgcg acctgtgcaa
      781 ccatccggcc aacgtgatga cgccgacgcg catcgtgcgt gaggccaagg cggtggccaa
      841 agaatccggt gtgcgcctga aggtgctcga acagaaggat atggaacaac tcggcatggg
      901 ggccctgctg ggtgtggcgc gtggcagcca cgaaccgccg aagttcatca tcctggaata
      961 caagggcgcc aaagcgaaga agggagatcc tccggtggtg ctggtgggaa agaccatcac
     1021 ctttgacacc ggtggcattt cgctgaagcc ggccgaaaat atggaacata tgaaggctga
     1081 tatgacgggc ggcgctgaag ttctggcgac gatgcgggcc gctgcgcggc tcaagcttcc
     1141 tctccacctc gtcagcattc ttcccgtggc cgaaaatatg ccgggcgggc gcgcgatgcg
     1201 gccgggagat atcgtgacga ccctctccgg taagaccgtc gaagtgcaga ataccgatgc
     1261 cgagggccgg ctgatcctgt cggacgcctt ggcatacgcc acccggtaca aaccggcggt
     1321 gttgatcgat atcgcgaccc tgaccggggc ctgcgtggtg gcactcggcc aatttgccat
     1381 cgggatgttc ggcaacaacg atcagttgaa agaacaggtc cggaacgccg gtcaacgcgc
     1441 aggcgagcgc gtgtgggaga tgccgttgtg ggaggagtat ttcgagcagc tccgcagcga
     1501 tgtggcggat atgcggaata tcggcggccg gggcggaggg atgatcaccg ccgccttgtt
     1561 cctgagcaaa tttgtcggcg attgcccatg gatccatctg gacattgcga gcaccgactg
     1621 gagcgaacgt gagcgagcgt atctgccgaa gggtccgacc gggatcggca cacgcctgct
     1681 cattcagttc ctcctggacc gcacgttgcc gtagggcctg cttctcacca gatcgtcgga
     1741 gtagcgagtg atgacattac gcgagcagat cggtcagctg tttatgatgg gcttcaccgg
     1801 gaccacggtc accaaagacc tggcctcgtt tctgacagct tataagccgg gcggcgtcat
     1861 ctttttccgc cgcaatctcg agtcggttca gcagatcgtc gatctcacca acgggctgca
     1921 gaaactatcg cccgttcggc cgctgctgat cgcgattgac caggagggcg gccgggtatc
     1981 ccggctgccc gccgaattta cgattttccc tccctgcgga cagctggggc agtgcaactc
     2041 cagtgaactg gcctactccg ctgccgcgac cattgccaaa gagttgcggg cggtggggat
     2101 caatatgaac atggctccgg tcctggatgt gaacagcaat ccggacaatc ccgtcatcgg
     2161 cgatcgggcg ttcggcgctg ctcccgagct tgtcggcgag atggggtcgg cgaccatcgg
     2221 agggctgcag gacaatatgg tggtcgcatg cgggaagcac ttccccggcc atggagacac
     2281 ggcgacggat tcgcacaaag agcttcccgt cgtggatgcg gggctccagc ggttgcgcga
     2341 tacggagttt cctccctttc agcaggccat tcgttatggc gtagccagtt tgatgaccgc
     2401 ccatgtgctg tatcgggcct tggatccgga ggcaccggca accctgtcct cggcggtgat
     2461 ccagcgcttg ttgcgcgaag agtttcggta tgagggcgtg gtttttaccg acgatctgga
     2521 gatgcatgcc attatcgatc acgacgggat cggcgaagcg gcagtccggt cgtttgtggc
     2581 cggatgcgat gttctgttga tctgcaagga ccaggaccgt gttatgaccg ccatgcaagc
     2641 gatggagcgt gcggtgaaag atggcaggat cactcaggag aggctggacc agtcgctggc
     2701 ccgtgtggcg aagctcaaag cacggtacct ccatccctac aagccggtca cgatttccga
     2761 tgcacgactg gtcgtcgggt gccgctcgca caaggtgctg ctcgattcat ggcacaagac
     2821 ctatgcgcgc atacccaagc cgacgtcatc tgatacggct caggctgcac cagcccatga
     2881 ttctccagtg acgcacgtgt gatctgcgtg cgccctgttc cgttcgcctc tcgatccagg
     2941 cacgggggga gtgggtgttc tttttctggt caattcatgg cgccggcctg atttctacag
     3001 cgcgatccag gctctccata tcctgggctc ggccgataga gtctgcgctg gcttttcccc
     3061 cggcgatctg attgactatc gaatcatgtg acgtttcccg tctttccttt cataccgagg
     3121 cggcatggca atcaaaaaag gtgacattct cgacgagcga aagcgctttc cggattcgtg
     3181 cgggttggtg gtcaaggtgg ctgggggcgg cgagggtttc cagaagatcc tctgttgtgg
     3241 gcatgagctg actgaagaag atgtggtctc cgatatcatc tcgtcgagag gccggaaaaa
     3301 aggcgcattg ctccccgggg ctatgctgga ggaaaagcgg cagttccccg actcctgcgg
     3361 cctgcgcctg atggtgatcg acggcggtgc cgggtttcag gggatcgatt gttgtggcca
     3421 caccatcaca gccaaggcgg tcaatggttt gaaattcggt caaccactgg aggattcccc
     3481 catgcctacc ggtcaggcag gaacagcatg acgccgccgg tgacccctcc cgggcctttg
     3541 gagaagtctc gccgggcaac catgcagtgg ttgctgggat tcatggatca cttagaccgg
     3601 ttgggctatg tcgcggcggg attcagcttg ctggccttag gcatgctgat ttttgttcat
     3661 gcctggtacg cctttttctt gcgctccaac gatgtcccgc tgctgcctgc cggactcaag
     3721 ttgttgaacg atctgctgct cgtcatcatc ctgctggaat tgttccgtac ggtggtgcgg
     3781 tttcttcaga ccgaggtgtt ggaattggag ccctatctgg ctgtcggcat catcgcctgc
     3841 acgcgacggg tgttgacggc cagcgcggaa ttatcccatc agcttgaggt ggtgacgcga
     3901 gaggcgcggc aggaactctt ccagcagtat gtgatggatg tcggcctcaa cgtgaccgtt
     3961 atcattgtcc tcatcttggg ggtgtacctg ctgcgcatgc gccctacgag agagcgcccc
     4021 gttacgacct aatcgcttct ggaccaaata gcaggggggt gataggtcca tcttgccctc
     4081 ttcccgcctc ttatggcatc atgcacctct tcttggaggc gcggtggcca cattgctcga
     4141 atatgtgggg tcagtgcata tctatctcgg gccgtaccgg ggaaacccga ttgccctcta
     4201 tttgaggcgt acggagaccg ggtgccagat tggtccccgg gcctatccct ggaatgacgt
     4261 ggtgggtgcg ggagaaaccc cgaacaaagc ggctgcagat ttcgaagaaa agtggaaagg
     4321 caaagggctg gccgccgata tgtattcagg tccttcctgg gaaggcggga ttaaaccgga
     4381 gcggcctgct ccgccgaagc cccctgctcc tcccaaacct gcggctgctc cgcccgctcc
     4441 tgcggcagca ggtacctcga ctccggcgcc agcatctcct ccacctgcgg cggttgccaa
     4501 aaagcctgcg gccccggctg cagcagcacc tccgacaacc gccccggttg ctccaaccga
     4561 agcgtcgtcc tagtttttcc cgtcaccagt ttcttggctc gtaattcgac tatgccggtc
     4621 gaggtgtctc ggactgttct gtcgatggcc ttgtggcgag tttgctatgg cgcatcccgt
     4681 acatggtgta gaccaggatg ccgatgatgg tccacacgaa aaaccggatc caggtcatcc
     4741 agggcaggaa atacatcagg cccaggcagg ccaggactcc caaaatgggg acgaccggca
     4801 tgaatggcat acggaagggg cgtggatggt tcggcttcgt gtagcgcaag accatgatgc
     4861 cgatacagac gaggacaaag gcaaagaggg tcccgatgtt cgtcatatcc gctgcctcac
     4921 cgatcggaat gagtgcggcc atgatggcga cggccacgcc ggtcaaatag gtggcgtggt
     4981 ggggcgttcg aaacttcgga tggacgcctg aaagccaggg acccaggaga ccgtcccgcg
     5041 acatggcgaa aaagacccga atctggccca gcatcatcac caccaggacg ctggtaatgc
     5101 ccgctaccgc accggttgcc acgatggcgg ctccccactt gaatccgacc atgcggagcc
     5161 cttccgctac cggggcatga acgtcgatct gcgaataagg cactaatccg gtcaggatcg
     5221 cggccacaga aatatagagc acggtgcaga tggccagcga ggcgaagatc ccgatgggta
     5281 aatcccgttg ggggttcttg gcctcttcgg cggtggtgga cacagcgtcg aatccgatat
     5341 aggcgaagaa cacgatggct gcggccgcgc cgacgccggc aaatccgaac ggcatgaagg
     5401 gggaccaatt agccgtgtcg acggaggatg tgccgaccgc aatgaagaag gcgatcacgg
     5461 ccagcttgat gagcacaatg ccgcaggtgg cgcgcgcgct ctctttgact ccgacgatca
     5521 ggatgccggt gacgagaagc acgatgatcg cggccggaat gttcgcaatg ccgccgtccg
     5581 cgccgggagg atgagtggcc cagtatggga gttcaaggcc gcagagtttg aggatgttgt
     5641 taaagtatcc ggaccacccg atcgcgacgg ccacgcaggc cacgccatat tccaggatca
     5701 gattccagcc ggtcagccag gccagaaatt ctcccaaggt ggcataagaa taggtatagg
     5761 cactgccggc gaccgggatc atggcggcaa attcggcata gcagagggcg gcgagcgcgc
     5821 aggtgatgcc tgaaagaatg aacgacagca tgatgccggg ccccgcgcct ggccggtgcg
     5881 catcaccgac gatagcggtg ccgatcagca cgaagatgcc ggtgccgata atcgcgccga
     5941 tgccgagtcc ggtcaggtcc caggccgtca gggtccgttt caggcgatgt tcaggggcgt
     6001 cggaatcagc gaggattcgt tcgatggatt tggtgcgaaa cagcggattg cccacgcagt
     6061 gtcctctctc cggcggcgcc acgtccatgt ccgctgttat ggcgaaatga accccgtgtc
     6121 atgagttgtt cctgcgtctc gcaggcgaag gttggagtga cgggcggcgc gaggcggccc
     6181 gcaagaatgt ctgaaagagt cgtcggtgca agaggtggcg ttcgaacaaa aattcggggt
     6241 gccattgcag gccgagaaag aaggggtgcg tcggaagttc gatcgcttca acgatgccat
     6301 ccggcgcggt tgcgctggca ataagggcgt gcccgaccgc tttcaccgat tggtgatgcg
     6361 agctgttgac ctgcatgcgg gcgcgcttga cgatgcgatt gagcaggctg ccgggtgtga
     6421 tggagatgct gtgagagagt cgaaccgccg gtgtcgtctg tcggtgctgt aacacatggt
     6481 cgacctgggc gggaatatcc tgatacaggc tccctccgca ggcgacattc atgctttgca
     6541 tgcctccgca aatagccagc gtggggatgt ggttccggat ggccaaccgc accaagtcca
     6601 attcaaagct ggagcgtcgt ttagcgacga ccggaaaggt atatcgcttt ttttctccgt
     6661 acagggttgg atccaagtcc ggcccgcttc ctgtcaggag gagtccgtcg accccttcca
     6721 gcaggcgccg gcgtgcggat tggttcgcca caagtggcag aatcaccggc acgccgccga
     6781 gttcctcaat ggcgcggaca taccgggccc gcaaaaaata tgtgggctcc ttgccgcccc
     6841 attcttgacg atcaccggca ttgaagtcgg gggtgactcc gatgacgggt ttcatgctag
     6901 cggatcggtt ccaaaggcaa cggcgctggt tcttgcgggg tgtaatcgcc ggcctctcgc
     6961 gcaggctccc ctgagacgcc gaggaagcgg atgggtttat cgcccttgag atggtccggc
     7021 gtgatgatgt aggtgccgta catgctgggg atccagatat tcttggccgt ctgttcactg
     7081 cctccggaga agccataggc caatccgccg acgaccccgc cgccgatcgc gaaggccagt
     7141 ttgagcggga aatagacgat ggtggccagt gcggagcccg cttgaatgcc gacacccgac
     7201 ggactggcat cgttcgacga gaccggcaca ttagacgcac ctcccgattc ctgcgcggag
     7261 acgggggcga tgagtaaggc cagagagcag actgtgacca gactgaagac gaggacttgg
     7321 gcccagaggc tgcgtcgtct tttaaggggc gaatagacag agacgttcac gttgggtgcc
     7381 tccttcgtgc tgaggttctg atgcggtgga aagaaaaata aatgccgatg agtccgttgt
     7441 cgttataaca aattcccccc cgttttcaaa ccccttccgg ctttgctgcc acaccgttgc
     7501 tcattagatt tcggaagtct cacccaggtc cagttcgagg cgaattcgat cggtcacctg
     7561 ttccggttgt tcgtcgagga gttgatgcag ttcgtcggcg aacgtggcgg cgtcgatctg
     7621 ttgattcacc tgttcaattc ccgcctggag ggccaagttg agcgcactgg tacagagcga
     7681 ctggacgaag agatcatcgg cgcggctggt cagctcctcc cgctcctctt ggtggtcgct
     7741 gtgtatgagt tcagccagcc aggaggcata cccaattcgc tcgcgggccc ggtcgaggtg
     7801 acgcccggcc acatctacag ccacttgcac gccgcctttc cagctccgct tcttcacgtt
     7861 gaagacgcat cgcagatgat ccggtcgctc ttcgacggct tccggcccga agaagaaggt
     7921 gacgcggtac tgcgcgggat cggatgaggg aacgatagcc atagcgggtt cggctcattc
     7981 taacattgcg tggagggtag agaacagcgt gattgcccag tggtatcgat ggcggaatga
     8041 cgctatgatg gcaagcatga acgtggccga tcgttctcac gcacgggtga ttcgtattca
     8101 gcaggccatc cgggagcagc cgggacttga tgggtggctc ttttacgact ttcgccacct
     8161 cgaccccatc gcgtatcgcg tcttgttgtt agacccctcg ctccatgtca cgcggcgttg
     8221 gtactattgg gttcccgcgc agggtacgcc ggtgaagctc cagcatcgca ttgagcctca
     8281 tgtcctggat gggttgcccg gagacgcgca tgcgtatgtc tcttggcgcg atcagcaggc
     8341 ggctcttggt tcattgcttc actctgctaa gcggattgcg atgcaatatt cgccgatgaa
     8401 tgccattccc tatctctcgc gagtggatgc cggaacaatt gatcttgtgc gcagtctcgg
     8461 cgcggaggtg gtgacctctg ccgatctggt tcagcaattt gaggcggtgt gggatgatgc
     8521 acaattggcg tcgcatcagg tggcagccga aggtctacgc gcaattgtcg acgaggcgtt
     8581 tggatttgtc ggaacttcgc tggctgctcg gtccagcctg acggaatatg gattacagca
     8641 atacattctt tcccgcatgc aggcccgtgg cttggtaacc tcgagtccgc cgattgctgc
     8701 ggtgaatgcg catagcgccg atcctcatta cgcgccggct tccgaaggat cagccccgat
     8761 tcggcagggt gaccttgtac tgatcgacct gtgggctaaa cagcctggac caggggcggt
     8821 ctatgcggat atcacctgga cggggttcgt cggggcgact gtgcctgccc ggcaccagga
     8881 catttttcag attgttcgac gggctcggga tgcggccgtt acctttgtgc aaggtcgtgt
     8941 acgagccggt gaatgcccct atgggtggga agtggatgat gtctgccgcc gggtgattca
     9001 ggacgcaggg tacgggcaat atttcgtgca ccgaaccggc cattccatcg gtgaggaagt
     9061 gcatggcaac ggcgcgaata tcgacaatct ggagacgcag gatgcgcgcc ggttgttgcc
     9121 gggcacctgc ttttcgattg agcctggcat ctacctgccg gatgagttcg gtattcggag
     9181 tgaactggat gtgtatctct ccgcccgcgg cgcggtggtg tacggccagc cggttcaggc
     9241 cgatcttctc ccgattccca tccctgcgag ctaggttctt cggtgctatt cctgggccgg
     9301 ctgtggccgc ccacctttct tgacaccctt tgcatcgagc agctatcgtg ggagttcaga
     9361 gggccgaaga ggccctctga cgctggattg actcctatct gccgaggaag gatatcgaac
     9421 atgtttggca ctatgggatt ttccgagttg attatcatcc tggtcattgt gttgatcatt
     9481 tttggggccg ggaaacttcc gcaaatcggc gagggggtcg gcaaggcgct caaagggttt
     9541 aagaaggagg tgaatgatat tccacctccc gagaacgcta ccgaacagaa cgagatagct
     9601 gcaacgcctg ctcagatcca ggctcagccg gcggcagata ttccgcaggc cgcacagccc
     9661 gccaccgtag cgccggcggc atctgctcct aaagccacct ctccatacac accgggtcct
     9721 gagctgactc ccggtacgac agcggctctg atggctgcgg ctgggccaca ggggccgcaa
     9781 gcgattcagc cagcgaaacc gcgtgtggcg acggtcacgc cggggcaagc ggcagcgggg
     9841 tcggcagttg cacaaagtca ccagcctccg acgatggaag accgtatggc ggctcctgcc
     9901 ccagtgatgc gcgcgcagta tccgcccctt ccgccaggcg cccagagcaa gccggtggct
     9961 aagcgtccgt cggcgattgt gaacaaggat gccgtggcgc gggtgcaggc ggctcaggca
    10021 gccatgcgaa caaaggcagc tcaggctcaa ccgtcgggcc tgtcagtcca ggacatgcag
    10081 ggtttggggg agggattggg ggatgccgtg cgcaccttcc gtcaggcggt tgcggatgtg
    10141 cgaagttcag tcgatcccga aatgcgaaca atccgggccg aaatggattc ggcgcagaag
    10201 gaactggaac agtccatcga ggcagccaag caggcacctg tgttggatga cgatgccccc
    10261 gcaaaatcgg tgtagcagag gctaataaat cttttcccct gcgcgggtga ttccggggcc
    10321 tcgtccaatt aggaagtgga ccggttcttt tttgctcgcg tcttgctggt tggtatcctg
    10381 ggcgatccac ccaagtgcct catctcgaac ctgcattcca atctcgtagt tacgtgcggg
    10441 cttaggttct tttctgcccg ctaggatgga ggtttcaatt ccgttgaatt ctttcactca
    10501 tatacgttcg gcttccccga tgcgttctta ttttcgactc caattcctct tctggctgct
    10561 gctgtgctcc gtggtgtatg ggtgttacgt cgatactcca tcagggactc cggagacggt
    10621 atctgggcgc ctcgttgagt tgcttgccga cccagactcg gccgtccggc gcactgccgc
    10681 agaggctttg gggaaggtcg gccataagtc ttccaatgcc agtctgatcg tggcgctcaa
    10741 tgatccggac gctcgtgtcc gcgaagctgc ggctctcgca ctgggtcggc tgggtgacgg
    10801 gaagagtggg atagcgctcg cagggcattt ggccgattct gctgagccgg ttcgaatggc
    10861 atcggcgctg gctctcggtg aaattgagtt ttctgcggat cgtgaggctc agactcttgc
    10921 ggttcttcgg catccgcagg gttctgctcg tattgccgca actcgtgcgt tgctcagcct
    10981 ggataccgtg tcgctctccg ctgatctgat caaagcgctt cgagatccgg atgcgcgtgt
    11041 gcgtcaggga gtcgcggccg tgctagggga gagcgggaat cctggtgccg tttcccacct
    11101 tcgctcactt ctcagaacag atggggcggc aagcgttcgc gcagaggccg cgtttcggtt
    11161 aggcaaaatc ggtgataacg gggtcttggc ggagctgtcg agggcggccg aggcggatcc
    11221 agatatgatg gttcgtggat gggctcgatg ggctgtgcaa cagattacgc tgtcgcgcga
    11281 gttcggttca gagaggcaac caagtcaatg agccgtgctt gtgcttccgg gtcgatatcg
    11341 gtgaactgaa tgcccattcc ggggaataac aagtatcgtt cgggttttga acgtgcccat
    11401 gccaccttcg cgcgcgtttc gaatttctga cttggccggt cgggcaaggc aaattcaacc
    11461 ttcaattcag tgccgggtgc taacggtgca ctactttcaa taaatagtcc gcctccgctg
    11521 atcccgcccg tcagactgtc gaagtgtttt ccatcgccgg tggtgcagtg taccttgatt
    11581 gcgaggggcg ctcggggatg cgcacggcta tgggcgaagc gtgcgtcttc gtcgaccgag
    11641 agcatgtgtt cgatcaacgt gccccaggta agtatgctga ggcgttggcc ggtgtcatcg
    11701 aatagagaca gggtttcttg gtcgcagtca atgctgagcg tcttcccttg gtgttcgcga
    11761 gtggcggtaa cgggaaactt catccttggc tcagatctct tgtgtgtgta ttggggagtt
    11821 tcgaattgcg gtgttgtata ggagttgttt actttggttc gtcctgggtg atgctcttga
    11881 cggaatgaac caggctgagt actcgattcc gtgtttccgc cgcgatgtcg ctgaagcgga
    11941 cgcccattcc cgggctaaat gtatattggt cggctttcgg gcaaacccac gcgatcacgc
    12001 ctttggccga caaccactct cccggacttt ccggcagagt aaactccatg gcgagcttag
    12061 ttcccacggc taagggcgta aagctctcga taaataagcc tcctccgccg atcccgcccg
    12121 ctcgactttc gaattgagtg ccctcggggg tgtggtattt gacccgtatt gagagtgaga
    12181 tgcgaggctc agatcgcgcc tcacgggact gctgaggttt ccggtaggtc agaatttgat
    12241 cgacgagaaa gtcccaggtc aggcttccca ttggctcgcc gtttatgccc accaaggtga
    12301 tcacttcttt ggaggaatca atctcaagcg ttttgccctt gtgtctattg ctggatacga
    12361 ctgggaattt cacatgcgcc gcccttcgga aaaggcacca gtggggggat atccatcttt
    12421 ccgcaagtat accgcacgaa atggtcttgt cgacaaaaac ctaagatttg gggaagtggg
    12481 ttggtgaagg cacgcctgaa gtgctgcccc gggggagcat aaaaagaggg ggcgcgaggc
    12541 cccctctgga aggagtaaag ctagtggtga gaataaactg ccacgggaaa gttatgcgct
    12601 cttcacgtcc ttatcctgct caaagatccg gatcggagcg tgttttccgc tgatagcgtc
    12661 ttcggttatg acgacttcct taatttgttt ctgcgaaggc gcatcgtaca tcacgtcgag
    12721 catggcctct tctagaattg agcgtaaccc gcgtgcaccg gtcttttgtg tgaaggcttt
    12781 ccgtgcaacg gcgctcaggg ctccctccgt aaaacggagc ttgaccttct cgaaggaaag
    12841 caacttttca tattgcttgg tgagcgcatt tcggggctcg gtaaggatgc gaatcaacgc
    12901 ctgctcgtcg agttcatcca gggtggcgac gaccggcaag cggccgatga actccgggat
    12961 caatccatac ttcaggagat cctccggttg gacatgcggc agcaattcac ccaatcggat
    13021 gtcgttgcgg cctcggactt ccgctccgaa ccccatcgat tttctgttca tgcgttgttc
    13081 gatgatatgc tcaagcccga caaatgcccc gccgcagacg aacaagatgt ttgaggtgtt
    13141 cacctgaatg aactcttgat gcgggtgctt gcgcccacct tgcggcggca cgttcgcgac
    13201 ggttccttca ataagtttca ggagcgcctg ctgaacgcct tcaccggaga catccctcgt
    13261 gatggaaggg ctatcgcttt ttctactaat cttgtcgatt tcgtcg
//
LOCUS       DAORQN010000033        13080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_289_length_13080_cov_23.880537, whole genome shotgun sequence.
ACCESSION   DAORQN010000033 DAORQN010000000
VERSION     DAORQN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_289_length_13080_cov_23.880537"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..2902)
                     /locus_tag="PK614_03710"
     CDS             complement(125..2902)
                     /locus_tag="PK614_03710"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRB81344.1"
                     /translation="MTPEAEARQRIDHKLERAGWVIQDLKQLNLSAGAGVAVREYPTD
                     TGPADYVLFVNRRPVGVIEAKKDSEGETLTVTESQTERYANATLKWRKDNAPLRFLFE
                     ATGQIIRFTDGADPAPRSRELFHFFTPDTLDAWQAQPKTLRRRLAEQMPPLPETNLRN
                     CQISAVTGLEQSLGLNRPRALIHMATGAGKTFTAITSVYRLLKFGGATRILFLVDTRN
                     LGKQAHQEFMAYTPPDDVRKFTELYNVQRLTSSTIDPHAQVCISTIQRMYSILSGEPI
                     DELAEDISLNEVQQTAKQTKVVRYNPAVPVETFDVIIIDECHRSIYNVWKQVLDYFDA
                     YLIGLTATPDNRTFGFFNENIVAQYTYEQSVADGVNVGYDVYEIETEVTQKGAKVKAK
                     EWVDHRDRQTRKKRWAETEEDTVYTGKELDRSVVNPSQIRHVVQAMKAAVEAQIFPAR
                     KETPKTLIFAKTDSHADDIINIVREVYGQGNAFCRKVTYSEKEADGILSSFRNDYNPR
                     IAVTVDMIATGTDVKPLEVLLFMRDVRSKGYYEQMKGRGVRSLDGDSLKRVSNSADGA
                     KTRFVLIDAVGVEKSLKTESRPLERKPTVPLKDLLQGVAVGHRDEDTVLSLANRLVRL
                     AKQLDDKAQARITKASGGITVAELGKRLIAALDPDAIVAAALATAQAHGITRTEETLL
                     PQEIETARATRVAAACAPFDQPGLRIEIEAARLEREQIIDHLNPDQITFSGFSEQAET
                     QAKEVIQRFADYIATHKDEIAALSFFYQQPYQRRQLTFEMIEELHEHLAKPPLLLTTE
                     RLWSAYARVQAGQVKGATSHRRLTDLVSLVRFALGLDSELKPFSDQVDKRFQEWIFRH
                     NAQRATAFSPEQTEWLRLMKDHIANSCSISREDFDYAQLADKGGLQKAWGLFGKELDT
                     LLNEMNEELVA"
     gene            complement(3705..4217)
                     /locus_tag="PK614_03715"
     CDS             complement(3705..4217)
                     /locus_tag="PK614_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81345.1"
                     /translation="MSATLSQVRLRDYVLSCGLLTIPILVWNMAFTGFLPPALGSQEF
                     WRDIPPMVTYGENLLRLIVLILPFLMPLDLRMVSQRRGLTLFTVGMVLYFLAWLALIL
                     FPQSRWSLSWPGFLAPAYTPLLWLAGLGLIGRRLYWPGPYRWWMYLWLAAGFTAFHVA
                     HTSIVYERNY"
     gene            complement(4221..4835)
                     /locus_tag="PK614_03720"
     CDS             complement(4221..4835)
                     /locus_tag="PK614_03720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRB81346.1"
                     /translation="MRTPLLGFSIALLEFYLVFWGSQILFPGGTAGTVILVLTTVAGV
                     VFVKPGWNIWPSRPAGCWAVLFGLLVGSAVLAASVLRLTKIPTPYDTATLNIVMILPG
                     IMTVTGVEELLFRQVLFRWLEQQQISARGVVLATSVAFGGAHLGPLLIGGDVSGPFYL
                     LQSLYMAWVGLLLGELRRASESWVMSWVGHAGYNVAVLVLLSQR"
     gene            complement(4858..5574)
                     /locus_tag="PK614_03725"
     CDS             complement(4858..5574)
                     /locus_tag="PK614_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81347.1"
                     /translation="MMRQPSTAWGDRRRYLAVAILSLGAIGLTGILSFSAHLVFQPYF
                     RSIPPVLAVSVISVLGVVSLGFLSSRRWFEMAVGRESLRGMAWSSAFATLFAVVIILA
                     DLMVVFPYQHVPPPQSLLFYPTMAYVAEIVFHTLPLAILLALLGPLFKGRNSTKLLWL
                     CIILASCPEPIFQLSWLSSEIAFSWADAYVGLHVFVFNVLQLSIFRRYDFVSMYACRL
                     VYYLYWHVIWGSVRQYLLPM"
     gene            complement(5598..6602)
                     /locus_tag="PK614_03730"
     CDS             complement(5598..6602)
                     /locus_tag="PK614_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB81348.1"
                     /translation="MKRRGTTPPFRGPDGKTLPNSIAEISYLRLGGFDQWVMIRGENI
                     ANPILILLHGGPGFPEMRLFRTFNAALEQHYTVVYWMQRGTSKSFDRRMPASSLTIEQ
                     FIADLDELVEMLRARFRKEKVVLYGHSWGSVLGVLYAARFPEKVAAYVGTGQIGEWAA
                     FETASYTFILAEAERRQHRKAIRELRAIGPPPPAFWDMVVERKWITRFVGIVRGMSLW
                     RFTRITLSGPEASIFDLPNILRSQLVTPKAMWQELIQVNLIKNAPALQMPVFFFLGRH
                     DRVVVPETSVAYFDVLRAPSKTLIWFEESGHEPPTEEPDKFNELMAKLVRPVVLTSSS
                     "
     gene            6970..7128
                     /locus_tag="PK614_03735"
     CDS             6970..7128
                     /locus_tag="PK614_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81349.1"
                     /translation="MYIPPGFNTVTPYFFVADAEAFVRFLVQGLGGTETLSNYATERS
                     DPKRPSQT"
     gene            7139..7366
                     /locus_tag="PK614_03740"
     CDS             7139..7366
                     /locus_tag="PK614_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81350.1"
                     /translation="MMVSEATERYKPMAAAYYLYVEDADASMQRALKHGAALEMEVSD
                     MPYGDRQGGVKDQHGNIWWISQRTVHEPYTP"
     gene            complement(7437..7829)
                     /locus_tag="PK614_03745"
     CDS             complement(7437..7829)
                     /locus_tag="PK614_03745"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRB81351.1"
                     /translation="MHPEILQYNKQQGLHYKEVCDVLAEEIDRNLPNAENKIWHAHPV
                     WFLDGNPIVGYSKQKPGIRLMFWSGADFEEVGLNVVGKKFKDASVFYSEVSEVTKTDL
                     RRWLKKARAIQWDYKNLVRRKGRLERLR"
     gene            complement(8030..8326)
                     /locus_tag="PK614_03750"
     CDS             complement(8030..8326)
                     /locus_tag="PK614_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81352.1"
                     /translation="MRTIGMRAIFDGPEVSVYEMRGGTHLILMRKDKVVEGDASFDLM
                     VDDLRATHQRLSFLGLAPSPIEACPSIDHEVFTISEPAGNVITFFSSHASGKPI"
     gene            complement(8613..9266)
                     /locus_tag="PK614_03755"
     CDS             complement(8613..9266)
                     /locus_tag="PK614_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HRB81353.1"
                     /translation="MSAIAGVILLFVGTYLHPLGADPNDAVAAFTEYAADHLWIASHL
                     IQLSGVIFMVVALLFLTQELEGAGGNGWSRIATGGAVASLGVGAVLQAVDGIALKVMV
                     DAWATAPTAQKEVTFQAALAVRQIEIGLASMGNLLFGLTAICYGMALLVDDTYPKWMG
                     GLAIVGGVGTTIAGVMMAYTGFSGPAMAINMPASSILLGWMLSLGVLMWRRGGIRPR"
     gene            9437..9817
                     /locus_tag="PK614_03760"
     CDS             9437..9817
                     /locus_tag="PK614_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRB81354.1"
                     /translation="MKYLLLVHHNEDRFKSMPEGTRKDMLAESIQLCHQLDSKGQYVH
                     ASPLQPEGTGTIVRVRNGKPMVTDGPFMETKEQLADYFLIDAKDREEAIRIAERVPEA
                     RIGTVEVRPVIAISGLPDTVKRES"
     gene            9952..11133
                     /locus_tag="PK614_03765"
     CDS             9952..11133
                     /locus_tag="PK614_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRB81355.1"
                     /translation="MSSIRLLVGTRKGAFILTSDGKRKQWSVDGPHFGGWELYHLKGS
                     PSDPDRIYASQTSSWFGQVIQRSDDGGKTWNPPGTKPEDLMGADGMPKGESNMFCYDA
                     SEKTGKPLTTHQHYDGTQRPWEFKRVWHLEPSLTDPDTVYAGVEDAAIFKSTDGGTTW
                     KELAGLRSAKGQLWQPGAGGMCLHTILLNTGQPDRMFVAISAAGSFRSDDTGQTWKPI
                     TKGLVAKYELPDPDAEVGHCVHCITMHPARPNVLFMQKHWDVLRSDNGGESWQEISGN
                     LPSDFGFPIAVHTHEPNTIYVVPIKSDSEHYPPEGKLRVYRSTVGGNEWEALTKGLPQ
                     QNCYVNILRDAMAIDQLDPCGIYFGTTGGQVYGSADGGDSWEPIVRDLPAVLSVEAQT
                     L"
     gene            11614..11979
                     /locus_tag="PK614_03770"
     CDS             11614..11979
                     /locus_tag="PK614_03770"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB81356.1"
                     /translation="MPKITPFLWFDGKAEEAASFYTSIFKNSKIENVSAVSATFYLDG
                     QEFIALNGGPQFTFTEAISFFVNCETQAEVDELWEKLSAGGTKQRCGWLKDKFGVSWQ
                     VIPSVLGDMLQDENEDKSG"
     gene            12179..12478
                     /locus_tag="PK614_03775"
     CDS             12179..12478
                     /locus_tag="PK614_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRB81357.1"
                     /translation="MSITVVWDTWLRPGAEAEGLRLTRQIWSDMRRFDGFLSHHILID
                     QHEPSHLIALARWRSLADADAVRERYKDSETIRRLTPLLARPRDRWIMQEDENRS"
     gene            12617..>13080
                     /locus_tag="PK614_03780"
     CDS             12617..>13080
                     /locus_tag="PK614_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81358.1"
                     /translation="MSRSRRPRLEQWTVKDYEELAPPQSLVPFVDRFWHGVVSATSPF
                     RDFTIVPDGCVDVVYEQLGSHVRCFIFGTVSHLQQFQFTPDAQYLGIRFRPGMGRHFL
                     DLHPRELTDQHVELPAFLGVRPDELAGETDFLAQSELLTHRLGTTLHRLDITP"
BASE COUNT         3043 a         3723 c         3584 g         2730 t
ORIGIN      
        1 attggcacaa aatgaaccag ttcatccttt gcgctaggca acggacagaa tgagcggagg
       61 ttaacatctg caatatgcga aatcggagcc atcgcccaac ttttcgggag cttctccgac
      121 tcgatcacgc caccagctcc tcattcatct cgttcagcag cgtatctagc tccttcccaa
      181 acaggcccca agccttctgc agcccgccct tgtcggccag ctgggcgtaa tcgaaatctt
      241 ctcggctgat gctgcaggag ttggcgatgt ggtctttcat gagccgcagc cattcggtct
      301 gctcagggct gaacgccgtg gcgcgctggg cgttgtggcg gaagatccat tcctgaaaac
      361 gcttgtccac ctggtcgctg aagggtttca gttcgctgtc gagccccagc gcgaaacgaa
      421 ccagtgagac cagatcggta agccggcgat ggctcgtcgc gcctttcacc tggccggcct
      481 gcacgcgagc ataggcgctc cacaggcgtt cggtggtgag tagtagcggc ggcttggcga
      541 ggtgttcgtg caactcttcg atcatctcga aggtgagctg ccgacgctga taaggctgtt
      601 ggtagaaaaa gctcagcgcc gcgatctcgt ctttgtgtgt ggcgatgtag tcggcaaacc
      661 gttggatgac ctccttggcc tgggtttcgg cctgttcgct gaatcccgag aaggtgatct
      721 gatccgggtt gaggtggtcg atgatctgct cccgttcgag gcgggcggct tcgatctcga
      781 tgcgcaggcc aggttgatcg aacggcgcgc aagcggcggc cacccgtgtc gcgcgggcgg
      841 tctcaatctc ctgaggcaac agcgtctcct cggtacgggt gatgccatga gcctgggcgg
      901 tggcgagggc ggcagccacg atggcatcgg gatcgagcgc ggcaatcaga cgtttaccaa
      961 gctcagccac tgtgatgccg ccggacgcct ttgtgatccg cgcctgggct ttgtcgtcca
     1021 gctgcttggc gagccgtacc agtctgttcg cgagtgagag cacggtgtct tcgtcgcggt
     1081 gccctacggc cacgccctgc agaaggtcct tgagcggcac cgtaggcttc ctctccaacg
     1141 ggcggctttc ggtcttgagg gatttttcca cgcccacggc atcgatcagc acgaagcggg
     1201 ttttcgcgcc gtcggcgctg ttgctgacgc gcttgagact gtcaccatcg agtgaccgca
     1261 cgccgcggcc cttcatttgc tcgtaatagc ccttgctgcg cacatcgcgc atgaacagca
     1321 gcacttccag cggtttgacg tcggtgcctg tggcgatcat gtccaccgtg acggcgatac
     1381 gcgggttgta gtcgttgcga aagctagaga ggatcccatc ggcctccttc tcgctgtagg
     1441 tcaccttgcg acagaaggca ttgccttggc catagacttc gcgcacgatg ttgatgatgt
     1501 cgtcggcatg gctgtcggtc ttggcaaaga tcagggtctt cggcgtttcc ttccgtgctg
     1561 ggaagatctg cgcttccacg gcggctttca tcgcctggac cacatgacga atctggctgg
     1621 gattgaccac ggagcgatcc agctccttgc cggtatagac ggtgtcctcc tcggtttcgg
     1681 cccagcgctt cttccgagtc tgccggtcgc gatggtccac ccactccttg gccttaacct
     1741 tggcaccctt ctgggtgacc tcggtttcga tctcgtacac gtcgtacccc acgttgacgc
     1801 catcggcgac cgattgttca taggtatact gggccacgat gttttcgttg aagaacccga
     1861 acgtgcggtt gtccggcgtg gcagtgaggc cgatcaggta cgcgtcgaag tagtcgagga
     1921 cctgcttcca cacgttgtag atgctgcggt ggcattcatc gatgatgatg acatcgaaag
     1981 tctccaccgg cacagccgga ttgtagcgga cgacctttgt ctgcttggcg gtctgctgca
     2041 cttcgttcag cgagatgtcc tccgccaatt cgtcgatcgg ctcgccggat aggatcgaat
     2101 acatgcgctg gatggtactg atgcacacct gcgcatgggg gtcgatggtg gatgaggtca
     2161 gtcgctgcac gttatagagc tcggtgaatt tccggacgtc atcgggcggg gtgtaggcca
     2221 tgaattcctg atgggcctgt ttgccgaggt tgcgggtatc gaccagaaac agaatacgtg
     2281 tggcgccgcc gaatttgagc agccggtata ccgaggtgat ggcagtgaag gtttttcccg
     2341 cgccggtagc catatgaatc aaggcccgcg gcctgttcag gcccaaggat tgttccaacc
     2401 ccgtcacggc gctgatctga cagttgcgca ggtttgtttc ggggagtggc ggcatctgct
     2461 cggcgagccg gcggcgcaag gttttcggtt gagcctgcca ggcatccaag gtgtcgggcg
     2521 tgaagaaatg aaagagttca cgcgagcgcg gggccgggtc ggctccgtcg gtaaagcgaa
     2581 tgatctggcc ggtggcctca aacagaaatc gcaacggggc gttatccttg cgccatttga
     2641 gagtggcgtt cgcataacgc tcggtttggc tttcagtgac ggtgagcgtt tcgccctcac
     2701 tgtctttctt ggcttcgatg acgcccacag gccggcggtt cacaaacaac acatagtcgg
     2761 ccgggccggt gtcggtcggg tactcgcgca cggccactcc cgcacccgct gacaaattga
     2821 gttgtttcag atcctgtatg acccagccgg cccgttcgag cttgtggtcg atccgctggc
     2881 gcgcctcggc ttccggcgtc ataggaagtc ttccccgttg aacggtgctc ggccgtgcgg
     2941 ctgcaaagcc gacgtaaatc ggcgccagtc taccagacag tcacctccgc agccagcgta
     3001 ggaatgccgc tggaaccaac ggcaccgcaa gcaagagaat gggacgcgtg tatcagggcg
     3061 ggctgaaggc tggcaagaaa aaggcccgga ggtgaatccg ggcccttggg tttggtggag
     3121 gcgaggcgct caacccatcg ttgatagcct atattggccc ctctctcacc ttcacaatcc
     3181 aagggacagt gcttgccgca tgacacctaa cccacaagtg aagcccatcg tatgatcaac
     3241 cagactctcc atcctaggca gatcgaacgc atcacagttt ctttatggta actgacgaga
     3301 tcggctgata gtggggtgaa aggttgtggc ccagtgtgta ttttcgagat cgatcgacct
     3361 atgaccttcc cttcagcgtc aaacgcaatg attagcccca tcccatcggt gaacacccct
     3421 tcaccgctca ccgcgacaat gaggcctatg ccaggcacaa ataacagcag gcttcgaatt
     3481 gcaccggatt catcgtcaca tcaacaatat taacaagata gttctgcaaa gcacggcaaa
     3541 accgggcaag aaatacgcaa atctggaacc ggacgcaacc aggaatcaaa ccctcaagac
     3601 ttgcctccat gcaaggggga tgactcatga gcactcgtct cgcccttccc tgtcgtcgtc
     3661 gttctttcgc ctggaactaa gcttccgacg ataagtgggg tcgctcaata attccgctcg
     3721 tagacgatgc tcgtatgtgc gacatggaag gccgtgaagc cggcggcaag ccagagatac
     3781 atccaccagc gatatggccc tggccaatag agcctgcgac cgatgagacc gagaccggca
     3841 agccatagga gtggcgtata tgctggagcg aggaatcccg gccaactcag actccaccga
     3901 gactgcggga agagaatcaa tgcgagccaa gccagaaaat acaacaccat cccgaccgtg
     3961 aacagggtca ggcctcgccg ttgggatacc atcctaaggt caagcggcat gaggaacggt
     4021 aagatgagga cgatgagacg aagaagattt tccccataag tcaccatcgg tggaatatcc
     4081 cgccaaaact cctgtgaccc taaggctggt ggcagaaaac ctgtgaacgc catgttccaa
     4141 actagaatgg gtattgtgag cagcccgcaa ctcaggacat agtcacgaag gcgaacctga
     4201 ctcaatgtcg cgctcataga tcaccgttga gagagcaaga ccaggaccgc cacattgtag
     4261 ccggcgtgac cgacccacga catcacccac gactcggagg ctcggcgaag ctcgccaagc
     4321 aacagcccaa cccacgccat atacaaactc tgcaacagat aaaatggccc gctgacgtcg
     4381 cctccgatga gcaagggccc aaggtgcgca ccgccaaatg cgaccgacgt cgcgaggacc
     4441 acgcctctcg cagaaatttg ctgttgttcc agccatctga acagaacttg ccgaaagagc
     4501 aattcctcca ctccagtcac agtcataatg cccggcagga tcatcacgat gttgagcgtc
     4561 gctgtatcgt agggtgtggg gatcttggtc agccgcagca cggatgccgc cagtacggca
     4621 cttcccacaa gcagcccaaa cagaacggcc caacacccgg cgggccgact tggccagatg
     4681 ttccaacccg gtttcacgaa caccactccg gcaaccgtcg tgagcacgag aatcacggtg
     4741 cccgctgtac ctccgggaaa gagtatctgg gacccccaga agactagata gaactcaaga
     4801 agggctatgc tgaatcccag caatggcgtt cgcatatgcc ttcgcatcct atcctcgcta
     4861 catcggcagc aggtactggc gcaccgagcc ccatatgaca tgccaataga ggtagtacac
     4921 cagccgacag gcatacatcg agacaaagtc ataccgccga aagatggaga gctgaagcac
     4981 gttgaacaca aacacgtgta accccacata tgcatccgcc catgagaacg ctatctccga
     5041 ggacagccaa ctcaattgaa agatcggctc cggacaggat gcgaggatga tacagagcca
     5101 gagaagcttg gttgaattcc gtcccttaaa gagcggtcca agcaatgcca ggagaatcgc
     5161 aagaggcagg gtgtgaaaca cgatttccgc gacataggcc atggtcggat aaaacaacaa
     5221 agactgcggc ggcgggacat gctggtatgg gaacacgacc atgagatcgg caagaatgat
     5281 caccacggcg aacagcgtgg cgaacgccga ggaccatgcc atgcccctca gactctctct
     5341 tcctacggcc atctcaaacc aacggcgtga agaaagaaaa ccgagagaga caactcccag
     5401 aacgctgatg acggagaccg cgaggacagg agggatactt cgaaaatacg gctgaaacac
     5461 aagatgggcg gagaaagaca ggatgcccgt gagtccgatc gccccaagag acaagattgc
     5521 aacggccagg taccgtcgtc gatcacccca ggccgtggat ggctgtctca tcatcgtcgg
     5581 attccaggaa ccaccggtca cgaagatgag gtcagcacga cgggccgcac aagtttcgcc
     5641 atcaactcgt tgaacttgtc cggttcttcg gtcggcggtt cgtggccgga ttcttcgaac
     5701 cagatgagtg ttttggacgg agcccgcaag acgtcgaaat acgcgacgct ggtttccggc
     5761 accaccacgc gatcatggcg accgagaaaa aagaagaccg gcatctgcaa tgcgggtgca
     5821 tttttgatga gattgacctg gataagctcc tgccacatgg ccttcggcgt tacgagttgg
     5881 cttcgcagaa tgttgggcaa gtcgaaaatc gacgcttccg gcccgctcag cgtgatacgg
     5941 gtgaatctcc acaaggacat cccgcgtacg atgccgacga agcgcgtgat ccatttgcgt
     6001 tccaccacca tgtcccaaaa ggcaggtggc gggggaccga tggcacgcaa ttcacgaatg
     6061 gccttgcggt gttgacgacg ctccgcttcg gcaagaatga acgtatacga ggccgtttcg
     6121 aaggcggccc actcgccaat ctgtccggtg ccgacatagg cggccacctt ctccgggaat
     6181 cgtgcagcat agagaacgcc caagaccgaa ccccaggagt ggccgtagag caccactttc
     6241 tcttttctaa aacgtgcacg gagcatctcg accagttcat cgagatcggc aatgaattgc
     6301 tcgatcgtca gggaggaggc gggcatgcgc cggtcgaagg acttgctcgt gccacgctgc
     6361 atccaataca cgacggtgta gtgttgttcg agtgcggcat tgaacgtacg gaagagacgc
     6421 atctctggaa agcccggccc cccatgcagc aggatcaaga tcggattggc gatgttctcc
     6481 ccccgaatca tcacccactg atcgaaccca cccaaacgta ggtagctgat ttccgcgatg
     6541 ctgttcggaa gggtcttgcc atcaggaccg cgaaacggcg gcgttgttcc cctacgtttc
     6601 attgcattct cttaattgag ccaagacaag cacacctatc cctctactac tcctccactc
     6661 ccaccatgtg agaccctgtg gcctctctcc acaggcaaaa gcttcttgct gtcgcgtatc
     6721 accccatgga tgttcctcaa acatcccgtc tctttgtaca agcacattgt atgccctttg
     6781 ttacgcagcg atcttcgacg aacaggcagg cagcatactc cttgcaaaac aagagccgga
     6841 acgacgattc ctggagatgc ggtccagcga actccgtgaa cgaggggcac tcaataggaa
     6901 ccttgtctcc gatccacgag tctacagcgc ctgcactgct gtgtactcat atcttccaag
     6961 aggtccgtca tgtatattcc gcccggcttc aacacagtca cgccctactt ctttgttgcc
     7021 gatgccgaag cgttcgtgcg cttcttagtg cagggtcttg gtggaactga gaccctgtcg
     7081 aactatgcga ccgagcggtc tgatccaaaa cgtccaagtc aaacttgata catccactgt
     7141 gatggtcagc gaagccacag aacgttacaa gccgatggct gctgcttatt atttgtacgt
     7201 cgaagacgcc gacgcgtcca tgcaacgggc gctcaaacat ggagcggctc ttgagatgga
     7261 ggttagcgac atgccctacg gcgatcgaca aggcggtgtg aaagatcagc acggaaacat
     7321 ctggtggatc tcacaacgga ccgtacacga gccctatact ccgtagggat gtacaaagat
     7381 tcataaggac tgtgctattt cgagagaact gctcgcggca ttctggcctc tgcatcctac
     7441 ctcagcctct ccagcctccc tttacgccga acgagattct tatagtccca ctgaatcgcc
     7501 cgagctttct tcaaccatcg gcgcaaatct gtctttgtta cttctgagac ttcactatag
     7561 aaaactgatg catccttgaa cttcttgccg accacgttca gtcctacctc ttcaaaatcc
     7621 gccccgctcc agaacatcag gcgtatacct ggcttctgtt tgctatatcc gacaatggga
     7681 ttcccatcca aaaaccagac tggatgcgca tgccatatct tgttttcagc gttgggcagg
     7741 tttctatcga tttcctcagc aagcacgtca cacacttcct tgtaatgaag tccttgttgc
     7801 ttgttgtact gtagaatttc tggatgcatg gcattcctcc agctgcatga caatgtttcg
     7861 gtcaaaccgt atacgagagg tatcaggcag tttcaatcca catgacacgc aatgaacgcg
     7921 tcgaataccc tacgccattg gggccaccgc gatgacgata acactctgac cccaatccgt
     7981 tacttgacca acgcctgctc gcagcgcttg ttgggcacca agacctatgt catataggct
     8041 tacccgaagc gtggcttgag aaaaatgtga tgacgttccc agcaggctca ctgatggtaa
     8101 aaacttcgtg atcgattgat gggcaagcct caatcggtga cggggctagc cccaagaatg
     8161 aaagccgttg atgagttgct cgtagatcat caaccataag gtcaaatgag gcgtcaccct
     8221 cgacgacttt gtctttgcgc atcaagatca gatgtgtacc accacgcatt tcgtagaccg
     8281 acacctctgg tccgtcaaat atggcgcgca tgccaattgt gcgcatgaac cgggcagtct
     8341 cctccatgcg atccgtattg agaacaacat gcgctacgcc cacctcagga cactgatcat
     8401 actcgctcat tttcctggtg cccaactgtg tataaaccga tccgcatgag cgcggtatcc
     8461 ggcagttcaa tacgcacaat acgcgacaaa cactcgcaca tcgtacgcct tccctgttgt
     8521 tgtttccacg agttgcaacc acacgatcat tcttcacaaa ttcttatacg gatcggcata
     8581 acgcgatagc tcagggtcag agtacaatac tgttatctgg gccggatccc ccctcggcgc
     8641 cacataagca ctccaagaga gagcatccaa cccagaagga tggaactcgc cggcatattg
     8701 atcgccatcg ctggtccgga aaaccctgtg tatgccatca tgacccctgc gatcgttgtg
     8761 cccacacctc ccacaatggc aagcccaccc atccactttg gatacgtatc gtcaaccagc
     8821 aaagccattc cgtagcagat cgctgtgagc ccgaacaaca gattccccat gctggccaaa
     8881 ccgatctcta tctgacggac ggcgagagcc gcctgaaagg tcacctcttt ttgcgctgtc
     8941 ggcgcggtgg cccaggcatc aaccatcacc ttcaaggcga ttccgtccac ggcttgcagc
     9001 acagctccca ctcctagact agcgacggct ccgccagtcg caatgcgcga ccacccgttc
     9061 ccgcctgcgc cctccaattc ctgggtgagg aacaacagtg cgacgaccat gaatatgaca
     9121 ccacttaatt ggatgaggtg gctcgctatc cataagtggt cagcagcgta ttcggtaaac
     9181 gccgcaacgg catcattcgg gtctgcccca agcggatgca gataggtgcc gacgaagagt
     9241 aatatcaccc cagcgatggc actcatggca ccaactctgc ttctgttcat ggcaacacag
     9301 ttatcccaag gaaacattcg ttacgccaaa atatcagtcg cttccaaaag cgtgttgctt
     9361 accaaacctc aggtaggcca cccaatgtcg attctcgcct gacccgacga ctatcttatg
     9421 ccatgggagg catgccatga aatatctgct gctagtccat cacaatgaag ataggttcaa
     9481 gagcatgccc gaaggcacca ggaaagacat gctcgcagaa tccatccagc tctgccacca
     9541 acttgatagc aaaggacagt atgtccatgc ctcgccccta cagcctgagg gaacaggaac
     9601 catcgttcga gtacggaacg gcaagccgat ggtgaccgac ggccccttca tggaaaccaa
     9661 ggaacaactc gctgactact ttctgatcga cgctaaggat cgagaggaag ctatcagaat
     9721 tgccgaacga gtacctgagg cacggattgg cactgtcgaa gtcagaccag taattgcgat
     9781 ctctggattg ccggacaccg tgaagcgtga atcctgaaac gtatcaagcc ttggggtgga
     9841 gttgcgggtt gcgggttgcg ggtttctcgt ctcgaaagta tcacgaaacc ttgaactcac
     9901 aacccgaaac atttgcttca cagatacgaa cgacgaagaa aggagcgcgg aatgagcagc
     9961 atacggctac tggtcggaac aagaaagggt gcgttcatct tgacctcgga cggcaagcgg
    10021 aaacagtggt ccgtcgacgg tccgcatttc ggtggctggg aattgtacca tctgaaaggc
    10081 tcgccgtccg atcctgatcg gatctatgcg tcacaaacca gtagttggtt tgggcaagtc
    10141 atccagcgat cagatgacgg cgggaagacc tggaatccac caggtaccaa accggaagac
    10201 ctcatggggg cggacggtat gccgaagggg gaaagcaata tgttttgcta cgacgcctcg
    10261 gagaagaccg gcaagcccct cacgacacat cagcattatg acggcacgca acgtccctgg
    10321 gagttcaagc gcgtatggca tctcgaaccg tcgctgaccg atccagatac cgtatacgca
    10381 ggcgtagaag acgcagcaat cttcaagtcg actgacggcg gtaccacatg gaaggagctg
    10441 gcgggactgc gcagcgccaa aggccaactc tggcaacctg gcgccggcgg catgtgcctt
    10501 cacaccatct tgctgaatac cggacagccg gatcgcatgt tcgtcgccat ctcggcggcc
    10561 ggttccttcc gcagcgacga cacaggccag acgtggaagc cgatcaccaa aggcttggtt
    10621 gcgaagtatg aactgcccga tccggatgcc gaagtcggcc attgcgtcca ttgcatcacg
    10681 atgcatcccg ctcgtccgaa cgttttgttc atgcagaaac attgggatgt gctgcgcagc
    10741 gacaatggtg gcgagtcgtg gcaggagatc agcggcaacc taccgagcga tttcggattt
    10801 ccaatcgcgg tgcataccca cgaaccaaac acgatatatg tcgtcccgat caagagcgat
    10861 tccgaacatt atccgccgga aggcaagtta cgcgtgtacc gcagcacggt cggtgggaac
    10921 gaatgggagg cactgacaaa gggactgccg caacagaact gctacgtcaa cattctgcgt
    10981 gatgcgatgg ctatcgatca gcttgatccc tgcggcatct acttcggtac caccggcgga
    11041 caggtttatg gttctgccga tggtggagac agttgggagc caattgttcg agatctacca
    11101 gcagtcttgt cggtggaagc acaaacacta tgatacaacg tgaagtgcta agcgtaacga
    11161 gttgcgagct tcgagttacg agttcctgaa ttcgaaacgt gaaactcgaa acacgaaaca
    11221 tcagaaggtg agacacgact catggtgcgt gtgattctcc cacaacattt gcggactctc
    11281 gcaagaatta gcggcgacgt tgcgttgggc gttaccggcc cagttacgat cgactcagtc
    11341 ctcgatgccg tggaggctcg ttatccaatg ctacgcggga ccatccgtga tcatgtaacg
    11401 aaacgccgtc gccccttcat ccggttcttt gcatgcgagc aggatctatc gcacgaagcg
    11461 ccagacactt tcctccccac agccgtgaca agcggagcag aaccgttgtt gattatcgga
    11521 gccatggcgg gcgggtgatt gcgctgccga tcttgagcta cttcgctcga acatgaagcg
    11581 gacaatagtt gatgaaaata ggaggaataa gcgatgccga agattacgcc gttcctatgg
    11641 ttcgacggca aagccgagga ggccgccagt ttctacactt cgatctttaa gaattcaaag
    11701 attgagaacg tttcggccgt gtctgcaaca ttctacctcg acgggcaaga gttcatagcc
    11761 ctgaacgggg ggccacagtt caccttcacc gaggccatct ccttcttcgt caactgtgag
    11821 acccaggctg aagtcgacga gttatgggag aaactttccg ctggcggaac caaacaaagg
    11881 tgtgggtggc tgaaagataa gttcggcgtg tcgtggcaag ttattccctc tgtcttgggc
    11941 gacatgttgc aggacgagaa tgaagacaaa tcagggtgag tcatgcagtc catgttgcag
    12001 atgagcaaga tcgatatcgc cggcttgaaa cgtgcctgga aggaggtgca tagaatgcta
    12061 ccggtgtcgt gttttttcaa gacgcttcct ctcggcaatc tgtagggtgt tattgctagt
    12121 accgagaggg agtaccccga tagtcagcgt tgcgttctgc aagagaagga ggacacccat
    12181 gagcatcacc gtcgtgtggg atacgtggct cagaccaggc gcggaggccg aggggctgcg
    12241 gctgacgcgg cagatctggt cggacatgcg acggtttgac ggttttttgt cacaccacat
    12301 tctcatcgat cagcatgaac ctagccatct catcgcgctg gcacggtggc gaagcctcgc
    12361 ggacgccgat gcggtccggg aacggtacaa ggattcagaa acaatccgac gactcacccc
    12421 gttgctggct cgtccgaggg atcgctggat catgcaagag gatgaaaacc ggtcatgagc
    12481 cctctgtaat ggtaggtcga catttcaccg ctccccctaa tgtggctccg attatgcgcc
    12541 ggttgagggc tagtaaagtc agcaggttga ctttcaatca ggacagcagg aggatcaact
    12601 caatctgagg ttccctatga gccgcagcag acgccctcga ttagaacaat ggactgtgaa
    12661 agattatgag gagctggcac caccgcagtc gctcgttccc ttcgtcgatc gattctggca
    12721 tggcgtcgtg tcggcgacga gcccgttccg cgactttacg attgtgcctg acggatgcgt
    12781 ggatgtggta tatgaacaac tgggctctca tgttcgttgt ttcatcttcg gaacagtcag
    12841 ccacctccaa cagtttcaat tcacgccgga cgcgcagtat ctcgggattc ggtttcgtcc
    12901 aggaatgggc cgacactttc ttgatctgca ccccagagaa ctgaccgacc agcatgtgga
    12961 gctcccagct ttccttggtg tgcggccgga cgaacttgca ggcgaaacag acttcctcgc
    13021 tcagtcagag ttgctcaccc acaggctggg caccaccctc catcgattag atatcacacc
//
LOCUS       DAORQN010000034        13018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_291_length_13018_cov_13.965054, whole genome shotgun sequence.
ACCESSION   DAORQN010000034 DAORQN010000000
VERSION     DAORQN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13018
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_291_length_13018_cov_13.965054"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /gene="ffh"
                     /locus_tag="PK614_03785"
     CDS             <1..1232
                     /gene="ffh"
                     /locus_tag="PK614_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRB81359.1"
                     /translation="ADVNFKVVKDFLDRVREKAVGQEVLKSLTPGHQVVKVVWDELRG
                     MMGGERSGISLASRPPTIIMMVGLQGAGKTTTSGKLARLFKSQGKRVLLVAADPRRPA
                     AGDQLASLGHDLGIDVHRFDQIDASRADVVRICQRGVERGQEQGYDLIVLDTGGRLHV
                     DDELMAELVAVKQAVNPHEVLLVADAMTGQDAVNMAGQFDQQVGLTGVILTKVEGDAR
                     GGAVLSIRAVTGKPIKFLGMGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQETFSR
                     EEAEAAQKKLTSSTFTLEDFRSQLGQMNRLGSFEQILGMLPGGQKLKDLANSGLPEKE
                     MKRVAAMIDSMTTRERRDHTLINGSRKKRIARGSGTSVQEVNRLIKQFLQARKIAKVM
                     SGGAGGRRQLAQMLRGM"
     gene            1339..1656
                     /gene="rpsP"
                     /locus_tag="PK614_03790"
     CDS             1339..1656
                     /gene="rpsP"
                     /locus_tag="PK614_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRB81360.1"
                     /translation="MAVHLRLARTGRHKRPMYRVIAADSRKPRDGRFLEILGIFDPLK
                     NPAVPVLKSERVLSWLRHGAQPTVTVRTLLKRHGVWKEFETEKSAKAEKTEKTEKSAK
                     SKK"
     gene            1667..2194
                     /gene="rimM"
                     /locus_tag="PK614_03795"
     CDS             1667..2194
                     /gene="rimM"
                     /locus_tag="PK614_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRB81361.1"
                     /translation="MATLDQAELVTIGRIERSFGVRGEARVRSLTDVPGRFESLGQVT
                     IVGPAGKTLDTRVTHVRPGGPTWIMGFEAFATPEQVAEFRGAFIQVPRGDSPALPADQ
                     YYQCDLIGMVVQDEAGTVLGCLEEVVSMSGNQNFLVRHDGKELLIPAAKQVVVAVDVV
                     GRVMTVRLPEGFGDL"
     gene            2196..2969
                     /gene="trmD"
                     /locus_tag="PK614_03800"
     CDS             2196..2969
                     /gene="trmD"
                     /locus_tag="PK614_03800"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRB81362.1"
                     /translation="MRCAVLTLFPDMVAPVLGQSILKRAQEKGLLEVSVQNLRDHTFD
                     RHKTADDVPYGGGAGMVMKAEPVLQAVDALRAHYQASHPETTLRVIIPSPQGRPFTQE
                     LAQSLAQDERVVVFVCGHYEGMDERVRLALHPEEISVGDYVLTGGELPALVMIDAAAR
                     LVPGVLGDAASAAEESFTEGLLEYPHYTRPAEVRGMAVPEVLVSGHHEAIRLWRRKEA
                     LRNTYLKRPDLLRDRELGSEDRRLLSEVMQESLVQVPVR"
     gene            2986..3399
                     /gene="rplS"
                     /locus_tag="PK614_03805"
     CDS             2986..3399
                     /gene="rplS"
                     /locus_tag="PK614_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRB81363.1"
                     /translation="MNRLERIQRSLTKKTTPKFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGTLNSETFTVRKLSYNVGVERTFPIHSPNVAKVDVVRQGRVRRAKLYYLRTK
                     KGKFAKVEDREFKVESKAQTAAKREAAAAATVAKA"
     gene            3500..4162
                     /locus_tag="PK614_03810"
     CDS             3500..4162
                     /locus_tag="PK614_03810"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRB81364.1"
                     /translation="MEARCRGYRHIAGLDEAGRGPLAGPVVAAAVLLPRRCRLPGLND
                     SKQVGESDRVRLFAEIVRRATGVGIGAATEAEIDRLNILQATRLAMRRALQALPLQPD
                     FLLLDAVTLSGLSVPQRSIIKGDGLSCSIAAASIIAKVTRDRLMVEYHRWYPQYNFAE
                     HKGYGTPEHLRLLREHGPCAIHRRSFAPVQQHCVGDARKLCSSVDQSESPLDRCATDA
                     DW"
     gene            4138..4524
                     /locus_tag="PK614_03815"
     CDS             4138..4524
                     /locus_tag="PK614_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRB81365.1"
                     /translation="MRDRRRLVGDEGEGRAEAYLRRQGFRILGRNVRSPLGELDLVAD
                     DQGVLVFIEVKRRRSGTYGGAIEAVDARKRAKLIQLAAQYLAQHRIHDRACRFDVVLI
                     QDDSGTTEPVQHIANAFDVAGDDLRW"
     gene            4627..5295
                     /gene="ftsE"
                     /locus_tag="PK614_03820"
     CDS             4627..5295
                     /gene="ftsE"
                     /locus_tag="PK614_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRB81366.1"
                     /translation="MIQLFHVSKYYDRRLALSDVTLEIEKGEFVLLMGPSGAGKSTLL
                     KLLIGAERPEEGQILVQGRSLSKLRSSEIPLLRRKIGVVLQDFRLLPKKTVFDNVSLP
                     LLVQGASTVEIRRKVTDALRSVGLDHKKDLFPPGLSTGEQQRICIARAIVNGPIMLLA
                     DEPTGNLDPELTSEIIELFKSINARGTTIIVATHDPNVLAQVNRRVITLDQGKVVSQE
                     QVCS"
     gene            5286..6191
                     /gene="ftsX"
                     /locus_tag="PK614_03825"
     CDS             5286..6191
                     /gene="ftsX"
                     /locus_tag="PK614_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRB81367.1"
                     /translation="MLVRRVLYLLREAVANVLTNRTTTVVAVATTAFTFACVGVFLLL
                     YVNLRTMASSLEQDIQVMVYLQDDLTEQTRNEIELQLKADRAVGSLTFVSKERALADF
                     QSQFPAESHLLQGLGQNPLPASFVVTLAVESRSSDAMRRWANRTQSIPGVAQVQYNQE
                     WVEALAGIVRYIEVAAIIVGLILSAASVTIIANTIRLALYSRREEIEILGLIGASTTF
                     IRVPYLLEGAALGLLGSALSLVILKAGFELFRHEIRSASRFLGVDALLTFFPFQMCVV
                     LVCVGLFLGCAGSFLSLLRFGEGRA"
     gene            6188..7378
                     /locus_tag="PK614_03830"
     CDS             6188..7378
                     /locus_tag="PK614_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRB81368.1"
                     /translation="MKWARWGCVVVAAHLVLWTVPAWAERKDSYADKIEQEKKTLEKL
                     RGTIVEKRKKADEAEKKRESVLQGLQSLDERLVRYRQEHQDVVKKLKKKDVEIEQMSV
                     QLSRLSERIDERQDAIAARLRVQYVEGRFWHLRTLLAASSSRDFQRRFRYLSAVTQRE
                     YEIMETYRKDAERIAEVERSREEARQGILAYKASTEEKLAQIQGLKKQKRVYLAKITQ
                     EKESHDRAVEELERSANRVDSLLKELETRRRAALATRPPSATGGLRALRGTLLWPTEG
                     QVVSYFGRQKHPTFNTYIQRKGIEIRAAEGSNIRSVLAGQVVYADWLKGYGLVIIMDH
                     ANGVFSLYAHASKILTAVGARIEAGEAIGETGDTGMTGENTLYFELREGAEPVDPLVW
                     LSKR"
     gene            7405..8754
                     /locus_tag="PK614_03835"
     CDS             7405..8754
                     /locus_tag="PK614_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRB81369.1"
                     /translation="MEQRRSRRWLYLLLMVTVALGIGIVLEKGLERTGHASETYEELR
                     TFSEVLTQVQKHYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFG
                     GVGIQIGVKENRLAVISPIEGTPAHRAGIKAGDFITKVNDEPTKDLTLMDAVQKMRGP
                     KDTKVNLTIQRDGTADPLAFSLVRDTIKIESVKFKVLDNTIGYVRLTQFQEATGRDLS
                     RALKQFKEQKVQGTILDLRNNPGGLLTAAVDVSEQFVGNGKLIVYTKGREGKKDEWFS
                     KTKETLEDSPMIILVNEGSASASEIVAGALQDWGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVVKLQTQTVAKAGEKDGKESEPKTAKAPAAG
                     KDPSPSAKPGEEPAHKNGAVSAGDGAGDISLDDDVQLQKAVELLKTWKIFKELRPAA"
     gene            complement(8729..9220)
                     /locus_tag="PK614_03840"
     CDS             complement(8729..9220)
                     /locus_tag="PK614_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRB81370.1"
                     /translation="MDDRTHQLAQIFRAMANLLASQRANPYRVRAYRKAADSILTVTG
                     DLADLASRHQLQEIPGIGRDLAVKIEEFLATGTIRAYEELKTPLPEEVAGWASLPGLS
                     DALVSYLYFRLNIRTLADLESLVASHLLRTQPGFSGSEDTLLAAIRERIGTVPTTPPA
                     AAP"
     gene            9361..9555
                     /gene="thiS"
                     /locus_tag="PK614_03845"
     CDS             9361..9555
                     /gene="thiS"
                     /locus_tag="PK614_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRB81371.1"
                     /translation="MNGESRGIGDGETVAALLHELEIRADRVAVELNLEILDRKDFET
                     RGLREGDRVEILSFIGGGAR"
     gene            9564..10343
                     /locus_tag="PK614_03850"
     CDS             9564..10343
                     /locus_tag="PK614_03850"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRB81372.1"
                     /translation="MDTINDRLVIAGREFKSRLWVGTGKYKDFVETKKAIEASGADVV
                     TVAVRRVNITDRSKENLLDYLDPKKYIILPNTAGCYTVEDAVRYARLARAAGVSDLVK
                     LEVLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAA
                     PIGSGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQ
                     DPIAMAEAMKYGVWAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            10473..11099
                     /gene="thiE"
                     /locus_tag="PK614_03855"
     CDS             10473..11099
                     /gene="thiE"
                     /locus_tag="PK614_03855"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRB81373.1"
                     /translation="MPSVDFRLYLVTDRQQTAGRPLVSVVGRAVSAGVRAVQLRERDL
                     PIRELLSLALDLQRELSDMQVFINDRVDLAVALGCRGAHLRESSLPAPVVRTLLQPSQ
                     LLGLSVHSIQDAVAAERHGADFVVLGPIYDTPSKREYGAPLGLQVLEQAARAVNIPIF
                     AIGGMTASRTREVRQAGAFGVAVLSSILSASNVEAATEKFLSAIERES"
     gene            11255..>13018
                     /gene="arc"
                     /locus_tag="PK614_03860"
     CDS             11255..>13018
                     /gene="arc"
                     /locus_tag="PK614_03860"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HRB81374.1"
                     /translation="MAESKGSSGRLRSFRDSVKRLTKSLSDGDGVVTHKNDEQAREVE
                     KLRIQIQSMEEEIRRLYQSRYQLEQTTKQNEKLVATLQEAKSQIETLRAEVEKLTAPP
                     STYGIFSSLDTDGTGNVYVSGRKMKVSLHPSIKAKSLRKGQEVILNEALNVIEVRGFD
                     VQGEVVRLKDVLEGNRALVTLHFDEEKVAELGEPLLSERLSVGDHLLYDPRSGCVIEK
                     LPKSEAEELVLEEVPDVDYEHIGGLQKEIEQVRDAVELPFLYPHIFANYKLSAPKGVL
                     LYGPPGCGKTLIAKAVASSIAKKLGHLKDKQLRSYFLHVKGPELLNKYVGESERQVRE
                     VFKKAKERADDGHPVIVFFDEMDALFRTRGTGISSDIESTIVPQFLSEIDGVERLTNV
                     IVIGASNRQDLIDPAVLRAGRLDVKVKVGRPDATAARDIFSKYVSTDLPFAEDDLKRH
                     GGDARALVDSLMNMTVDAMYATTDENKFIEVTYANGEKEVLYFKDFASGALIEGIVSR
                     AKKFAVKRAIAQEGTGLRAEDLIRAIREEFKEHEDLPNTTNPDDWAKVAGKKGEKIVH
                     LRTISGGPAEARQIETVNTGHY"
BASE COUNT         2724 a         3348 c         4286 g         2660 t
ORIGIN      
        1 aggcggacgt caactttaag gtcgtcaagg atttccttga ccgcgttcgc gagaaggcgg
       61 tcgggcagga agttctgaaa agcctgaccc ccgggcacca ggtcgtcaaa gttgtctggg
      121 acgagctccg cggcatgatg ggcggtgagc ggtccggtat cagtctcgcc tccaggcccc
      181 cgaccattat tatgatggtt gggttgcagg gagccgggaa aacgaccacg tccggcaaac
      241 tcgcccgctt gtttaagagc cagggcaagc gcgtgttgct cgtggcggcc gacccgcgtc
      301 gtcctgccgc aggtgaccag ttggccagcc tcgggcatga tcttggaatc gatgtgcatc
      361 gattcgatca gatcgatgcc tcacgcgccg atgtggtgcg tatctgccag cgcggggtgg
      421 aacgcggtca agagcagggc tacgatctga ttgtgctcga taccggcggc cggttgcatg
      481 tcgatgatga gttgatggcc gaactcgtgg cggtcaagca ggctgtgaac ccccacgaag
      541 tgctgctggt tgccgatgcc atgaccggcc aggatgcggt caatatggcc ggtcagttcg
      601 atcagcaggt cggcctcacc ggtgtcatcc tgacgaaagt cgagggcgac gcgcgtggtg
      661 gcgccgtgct gtcgatccgg gccgtgaccg gcaagccgat taagtttctc ggcatggggg
      721 aaaagctcga tgccctggag ccgttccatc ctgaccggat ggcgtcacgg atcctcggga
      781 tgggtgatgt gctttcgttg atcgagaagg cgcaggagac gttttcacgg gaagaggccg
      841 aagcggcaca aaagaagctg accagcagca cctttacgct ggaggatttc cggtcacaac
      901 tcgggcagat gaatcggttg gggtcgttcg agcagattct gggaatgctg cccggcggcc
      961 aaaaattgaa agatcttgcg aacagcggat tgcccgaaaa agaaatgaag cgggttgcgg
     1021 ccatgatcga ctccatgacc acgcgtgagc ggcgcgacca tacgctgatc aacgggagcc
     1081 ggaagaaacg gattgcgcgt ggaagcggaa cgagtgtgca ggaggtcaac cgcctgatca
     1141 agcaattcct gcaggccagg aaaattgcca aggtcatgtc gggtggcgcg ggaggccggc
     1201 gtcagttggc ccaaatgctc cgaggcatgt agcaggccac gagtgtgtcg agtgtgcatc
     1261 gattcgaggt gaagaagaga gtcctaaccg agcggacaca gtctagggct gaacgctcat
     1321 tactctgagg aggttctagt ggcggttcat ttacgattgg cacggacagg gcgacataag
     1381 cggccgatgt atcgggttat tgcggccgat tcacggaaac cgcgcgacgg gcgctttttg
     1441 gagattctcg ggattttcga tccgctcaag aacccggcgg tgccggtgtt gaagtcggaa
     1501 cgggtgctgt catggttgcg gcatggcgcc cagcccactg tgaccgttcg gactctgctg
     1561 aagcggcatg gggtttggaa agagttcgag accgagaagt ccgcaaaggc ggagaagacc
     1621 gaaaagaccg agaagagcgc aaagagcaag aagtagccgg catcacatgg cgacgctcga
     1681 tcaagcagag ctggtgacca ttggaaggat cgagcgatcg ttcggggttc gaggcgaggc
     1741 gcgcgtgcgt tccctcacag atgtgcctgg acggttcgag tcccttgggc aggtcacgat
     1801 tgtcgggcct gccgggaaga cgctcgacac ccgtgtgacc cacgtgcggc caggcgggcc
     1861 gacgtggatt atggggttcg aggcgtttgc gacccctgag caggtcgccg agtttcgtgg
     1921 agccttcatt caagttcccc gcggagactc gccggcgctg ccggccgatc aatactatca
     1981 atgtgatttg atcgggatgg tcgtgcagga tgaagccgga accgtactgg gctgcttgga
     2041 agaggtcgtg tctatgtccg gcaaccagaa ctttctggtg cgtcacgacg ggaaagaact
     2101 gctgattcct gcggcgaaac aagtcgtggt tgcggtcgac gtagtggggc gggtcatgac
     2161 ggtgcggctg ccggaagggt ttggagatct gtaagatgcg gtgtgccgtt ctgacattgt
     2221 ttccggatat ggtggctccg gtgcttggcc agagtatcct caagcgcgcc caggaaaagg
     2281 ggttgttgga ggtttctgtg cagaacctgc gtgaccatac cttcgaccgg cataagaccg
     2341 cggatgatgt gccgtatggc gggggagccg gaatggtcat gaaggccgaa ccggtgcttc
     2401 aggctgtcga tgcgctgcgg gcccattatc aggcttctca tccggagacg acgttgcggg
     2461 tgattattcc gtctccgcaa gggcggccct ttacgcagga actcgcacag tccctggcgc
     2521 aggatgaacg ggtagtggtg tttgtgtgtg ggcactatga ggggatggat gagcgcgtca
     2581 ggctggcgct gcaccccgaa gaaatctccg tcggcgatta tgtgttgacc ggaggggagc
     2641 tgccggcgtt ggtgatgatc gatgcggcgg cgcgcctggt ccctggcgtg ttgggcgatg
     2701 cggcgtcggc ggccgaggaa tcgtttaccg agggattgtt ggagtatccg cactacacca
     2761 gaccggcgga ggtgcgcggg atggcggttc cggaggtact ggtatcgggt caccatgagg
     2821 cgatccggtt gtggcgccgg aaggaagcgt tgcggaacac ctatctgaag cggccggatc
     2881 tcctgcggga tcgcgagctt ggatcggaag atcgacggtt gttaagcgaa gtgatgcaag
     2941 agagtcttgt acaggtacct gttcgttgag aggaggagaa ggactatgaa tcggctagaa
     3001 cggatccaac gatccttaac caagaagacg acgcccaagt tcgagatcgg ggatactgtt
     3061 cgtgtccacg tgaaggtggt cgagggtgaa aaagagcgta tccaggtgta tgaggggacg
     3121 gtcatcgcca ggaaaggcac gctcaatagc gaaaccttca ctgtgcgcaa gctgtcctac
     3181 aacgtcggcg ttgagcggac gtttcccatc cactcgccca atgtggccaa ggttgatgtc
     3241 gtccgtcagg gtcgtgttcg ccgggccaag ctctattatt tgcgcaccaa gaagggcaag
     3301 tttgccaagg tggaagatcg cgagtttaag gtcgagagca aggcgcaaac cgcagccaag
     3361 cgtgaggcgg cagcagcagc cacggttgcc aaggcctgat cgatcgtttg tgctgtgttc
     3421 cgcttgagac caggacagag gcgcacgttt ggctggttct tcgaacccga cgacaggggg
     3481 ccccaccgag gagtttgagg tggaggctcg gtgtcgcggg taccggcata tagccgggct
     3541 cgatgaggcc ggacgtgggc ctctcgccgg tcctgtcgta gcggctgccg tgctgttgcc
     3601 gcgtcgatgc cgcctgcccg gactcaacga ttcgaagcag gtcggcgagt cagaccgagt
     3661 tcgattgttc gccgaaatcg ttcgccgggc aaccggcgtc ggcatcggag ctgcgacgga
     3721 ggcggagatc gatcggttga atatccttca agcaacccgg ttggcgatgc ggcgtgctct
     3781 gcaggcatta ccgcttcaac cggacttttt gttgttggat gcggtcactc tttccgggct
     3841 ctcagttcct caacgttcta tcatcaaggg cgatggcctc tcctgctcca ttgcggccgc
     3901 ctcgatcatc gccaaggtga cgcgcgaccg tttgatggtt gagtaccacc gttggtatcc
     3961 ccaatataat tttgccgagc ataaagggta cgggactccg gagcatctcc gtctgctccg
     4021 ggagcacggg ccctgtgcga ttcatcgccg gagttttgcg cctgtgcagc agcactgtgt
     4081 cggcgatgct cgaaaattgt gttcctctgt cgaccagtct gagagcccgc tggaccgatg
     4141 cgcgaccgac gccgattggt aggggatgaa ggagaggggc gagccgaggc ctatctgcgc
     4201 cgacaggggt ttcgaatcct tggacggaat gtgcgctctc cattgggtga actcgacctg
     4261 gttgccgatg accagggggt gctggtgttc atcgaggtga aacggcgacg gtctgggacc
     4321 tacggtggtg cgattgaagc ggtggatgcg cgcaaacgtg ccaaattgat tcagcttgcg
     4381 gcgcagtatc tggcgcaaca ccggattcat gatcgtgcgt gccgatttga tgtcgtcttg
     4441 atacaggatg attccgggac gactgaaccc gtgcagcata ttgcgaatgc attcgacgtc
     4501 gcgggggatg atctgcgatg gtaggatggg aatcgcgctc ctgattcatc cggtcactgt
     4561 gtgtcgtgcc gggcggcggg ggcccgagag gtcactccgg tccggcggct gctcttgtca
     4621 ccatccatga ttcaactttt tcacgtctca aaatattacg accgccgttt ggcgctgtcc
     4681 gatgtgaccc ttgaaatcga gaagggcgag ttcgtgctct tgatgggacc gagcggcgcc
     4741 ggaaaatcga ccttgttgaa attgctgatc ggcgcagagc gccctgagga aggacagatc
     4801 ctggtgcagg gccgcagttt gtcgaaattg cgatcgtctg aaattccctt actgcgtcga
     4861 aagatcggtg tggtgctgca ggatttccgc ctgctgccaa agaaaaccgt attcgacaat
     4921 gtgtcgttgc ccttgttggt gcagggggcc tcgactgtcg aaatcaggcg gaaagtgacg
     4981 gacgcattac ggtcggttgg gctggatcac aagaaggatc tctttccccc cggcctctct
     5041 accggtgagc agcagcggat ttgcatcgct cgcgcgatcg tcaacgggcc gatcatgctg
     5101 ttggccgatg aaccgacggg gaatctggat ccggagctga ccagcgaaat catcgaactg
     5161 ttcaagtcga tcaacgcccg gggcacgacc atcatcgtgg cgacgcacga tcccaatgtc
     5221 ttggcccagg tcaatcgccg ggtgatcaca ttggaccagg gcaaggtggt ctcgcaggaa
     5281 caggtgtgct cgtgaggcgt gtgttgtacc tgctgcggga agcggtcgcc aatgtgttga
     5341 ccaatcgaac gaccacggtg gtcgcggtgg ccaccacggc cttcacgttt gcctgcgtcg
     5401 gcgtgttttt gttgttgtat gtcaatttga ggaccatggc ctcgtcgctt gagcaggaca
     5461 tccaggttat ggtgtacctg caggacgacc tgacggagca gacgagaaac gaaatcgaac
     5521 tgcaactgaa ggccgatcgt gccgtcgggt cgttgacctt cgtgtccaag gaacgggcgt
     5581 tggctgattt ccagagtcag tttccggccg agtcccatct gctgcagggg ctcgggcaga
     5641 acccgctgcc ggcctcattt gtcgtgacgc ttgcggtgga gtcccgttca tcggatgcga
     5701 tgcggcggtg ggccaatcgc acccagtcga tcccgggagt tgcgcaggtg caatacaatc
     5761 aggaatgggt cgaggccttg gccggcattg tccggtatat cgaggtggct gccatcattg
     5821 tcggcctcat tttatcggcg gcgtcggtca cgatcatcgc gaacacaatc cgcctggcgc
     5881 tgtattcacg ccgggaggaa attgaaattc tggggttgat cggggcgagt acgaccttta
     5941 tccgtgtgcc ctatttgctc gaaggtgcgg cgctcgggtt gctcggcagt gctctctcgc
     6001 tggtaatcct caaagccggg tttgagttgt tccgccatga aatccggtct gccagccggt
     6061 ttctgggcgt ggacgcgctg ctgacatttt tccccttcca aatgtgtgtg gtgcttgtct
     6121 gtgtggggct ctttctcggt tgtgccggca gttttctctc gctgctccga ttcggagagg
     6181 gacgggcatg aagtgggcgc gatggggatg tgttgtggtg gctgctcatc tggtcttgtg
     6241 gactgtccct gcctgggcgg agcgcaagga ctcgtacgcg gacaagatcg aacaagaaaa
     6301 gaaaacgctg gaaaagttgc gcggcacgat tgtcgagaaa cgtaagaagg cggacgaggc
     6361 ggaaaagaaa cgcgagtcgg tcctgcaggg attgcagtcc ttggacgaac gtctggttcg
     6421 ttaccggcag gaacaccagg acgtcgtgaa gaagctcaaa aagaaggatg tggaaatcga
     6481 gcagatgagc gtgcagctga gccggctgtc cgaacgcatc gatgaacggc aggatgccat
     6541 cgctgctcgc ttgcgggttc aatatgtcga ggggcggttt tggcacctga gaacgctctt
     6601 ggcggccagt tcttcccgag actttcagcg gcggtttcga tatctctcgg ccgtgacgca
     6661 gcgagagtat gagatcatgg aaacctaccg gaaagatgcc gagcggatcg ccgaggtcga
     6721 gcggagtcga gaagaggcgc gccaggggat cctggcctac aaggccagca ccgaggaaaa
     6781 gctggctcag atccaggggc tgaaaaaaca gaaacgggta tatctcgcta aaatcactca
     6841 agaaaaggaa tcgcatgatc gggctgtaga ggaactggag cggtctgcca accgggtcga
     6901 cagcttgctg aaggaactgg aaaccagacg tcgggcagcg ctggcgactc gtccaccctc
     6961 cgcgacggga ggccttcgcg cactacgtgg gacgctcctc tggccgaccg aagggcaagt
     7021 ggtgtcctac ttcggacggc agaagcatcc gaccttcaat acctacatcc agcgaaaagg
     7081 gattgagatt cgcgctgcgg aagggagtaa tatacggtcg gtgcttgcag gccaggttgt
     7141 ctacgcggac tggttgaaag gctacggact cgttataatc atggatcatg caaacggagt
     7201 tttttcgttg tatgcccatg cgtcgaaaat attgacggcg gtcggggccc ggatcgaagc
     7261 cggagaagcg atcggtgaaa cgggcgacac gggcatgacg ggtgagaata cgctctattt
     7321 tgagttgcgc gagggggcag agccggtgga tcccctcgtg tggctgtcga agcgataggc
     7381 ctcggaagga aggaaaccta cgttatggaa cagcggcgaa gccgacggtg gttatatctt
     7441 ttgctgatgg tgacggtagc cctgggtatc ggcattgtgc tggaaaaggg attggaacgg
     7501 accggccacg cctccgagac ctatgaagag ctgcggacct tctccgaggt cttgacgcag
     7561 gtgcagaaac actacgtcga cgatacaaag gtgaaagacc tcgtacaggg tgcgattcgt
     7621 gggatgctct ccacgttgga tccgcactca gcctatatga cgcccgagat gtacaaggaa
     7681 atgcaggtcg aaacgaaagg cgaattcggc ggggtcggga ttcagatcgg cgtgaaggag
     7741 aatcgccttg ccgtcatttc cccgatcgaa ggcacgcctg cgcatcgagc cgggatcaag
     7801 gccggtgatt tcattaccaa ggtgaacgac gaacccacaa aggatttgac gctgatggat
     7861 gcggtgcaga aaatgcgcgg ccccaaagac accaaggtga acctcacgat ccagcgggac
     7921 ggcacggcgg acccgctggc gttttcattg gtgcgggaca ccatcaagat cgaaagcgtg
     7981 aagttcaagg tgctcgacaa taccattggg tatgtgcgtt tgacgcagtt ccaggaagcc
     8041 acgggacggg acctcagccg ggcgctcaag cagttcaagg agcagaaggt tcaggggacg
     8101 attctcgact tgcggaacaa tcccggtggc ctgttgacgg cggccgtcga tgtgtccgaa
     8161 cagtttgtgg ggaacgggaa gctgatcgtc tataccaagg gtcgtgaggg gaagaaggat
     8221 gagtggttct ccaagacgaa ggagacgctg gaagattcgc cgatgatcat cctggtgaac
     8281 gaagggtcgg ccagcgcgtc ggagattgtg gccggtgcgt tgcaggattg gggacgggcc
     8341 gtcattgtcg ggaccacgtc gttcggcaag ggatccgtcc agacgattct gccgctgggt
     8401 gacgggtcgg gactccggct cacgactgcc aagtactaca cgccgaaggg gcgctcgatt
     8461 cagtccaccg gcatcacacc ggacattgtg gtcaaactgc aaacgcagac cgtcgcaaag
     8521 gccggcgaga aagacggaaa agagtctgaa ccgaagacgg ccaaagcgcc tgcagccgga
     8581 aaggatccat cgccttcggc caagccgggt gaggagcctg cgcataagaa tggcgccgtg
     8641 tcggccgggg atggggccgg ggatatttcg ctggatgatg acgtgcagct tcaaaaggcc
     8701 gttgagctgt tgaagacctg gaagatcttt aaggagctgc ggccggcggc gtagtcggca
     8761 cggttccgat ccgttcgcgg atggcggcga gcagtgtgtc ttccgacccg gaaaaacctg
     8821 gttgtgttcg taagagatgg gacgcaacca ggctttccag gtcggccaat gtgcggatgt
     8881 tcaaccggaa atataagtaa ctcaccaggg catcggagag gcccggcaac gaagcccagc
     8941 cggccacttc ctccggcaac ggtgtcttca gttcttcata ggcgcgaatc gttcctgtcg
     9001 ccagaaactc ttcaatcttc actgccaagt cccgcccgat tccggggatc tcctgaagct
     9061 gatggcgcga ggctagatca gccaggtctc ccgtaaccgt caggatcgaa tcagccgctt
     9121 tccgatacgc gcggacgcga taaggattgg cacgttggct tgccaacagg ttggccatcg
     9181 cacgaaagat ctgcgcaagt tgatgggtgc ggtcgtccat agtcgtgtgc ccggcggtct
     9241 ttatctacat tgtgcgagta tcgaatccca aacggcaagg cctaaccggg cttggttgac
     9301 aaccggtttt cccgtttcat aggatgatct gaagaattga ggtgcgcagt gatcattcac
     9361 gtgaatggtg aatctcgcgg aatcggcgat ggggagacgg ttgcagcgct gttgcacgag
     9421 ttggagatcc gcgcggatcg cgtcgccgtc gaattgaatt tggagattct ggatcgcaag
     9481 gattttgaaa cccgcggact ccgcgagggg gatcgggtgg agatattgag ctttattggt
     9541 ggcggagcga ggtaaccaca tttatggata cgatcaacga tcgcttagtg attgccggcc
     9601 gggaatttaa gtctcgcctc tgggtgggga ccggtaagta taaagacttt gtcgagacta
     9661 agaaggctat cgaggcgtcc ggtgcggacg tggtgacggt ggcggttcgt cgcgtgaaca
     9721 tcacggatcg gtccaaggaa aatctcctgg attatctcga tccgaaaaaa tacatcattt
     9781 tgccgaacac cgccggttgt tacacggtgg aagatgccgt ccggtatgcc cggttggctc
     9841 gcgcggccgg cgtgtccgat ctggtcaagc tggaagtcct gggtgatgaa aagactcttt
     9901 tcccggacac cgcgggattg atcgaggcgg ccaagatcct catcaaagaa ggcttcatcg
     9961 tcctgcccta tacgaacgat gacccgatcg ttgccaagaa gctggtggat atcggctgtc
    10021 ccgcggtgat gccgctcgct gctccgatcg gctcggggct cgggatccgt aatccataca
    10081 atctcaagat catcatggaa acggtcaagg tgcccatcat cgtggacgcc ggagtcggaa
    10141 cggcatccga tgccgcgctg gcgatggagt acggagcgga tgcggtgctg atgaacaccg
    10201 ctattgccgg cgcgcaggac cccatcgcga tggccgaggc catgaagtat ggtgtctggg
    10261 ccgggcgact ggcctacaag gccgggcgca ttccgcgcaa gctctatgcg acagccagta
    10321 gcccgatcga aggcatgctc taatagccag agaaccgtct ttggtcggtg tcggcgagcc
    10381 gggctgagtg cgtgtgtgag tggtgaaccg gtcctgagct cccggcatct cacattcaac
    10441 tggcatcatt cagcatccag aatatttccc gaatgccctc cgtcgatttc cgtctgtatt
    10501 tagtgactga tcgtcaacag accgccggac gtccgctggt gtcggtcgtc gggcgggcgg
    10561 tgagtgccgg tgtgcgcgcg gtgcagctgc gtgaacgtga tcttccgatc cgagagctgc
    10621 tctctttggc gctcgacctg cagcgcgagt tgtcggacat gcaggtgttc atcaatgacc
    10681 gtgtggatct ggcggtggca ctggggtgcc ggggggcgca tttgcgcgag agcagtttgc
    10741 cggccccggt ggtgcgaacg ctgttgcagc cctcgcagct gttgggtttg tccgtccatt
    10801 ccatccagga tgcggtggcg gcagaacggc acggcgcgga ttttgtggtg ttgggtccga
    10861 tttacgacac gccttcgaag cgggagtatg gggctccgct tggcctccag gtactcgaac
    10921 aggccgcgcg cgcggtgaac attccaattt ttgccatcgg agggatgact gcctcgcgta
    10981 cacgtgaagt gcggcaggcg ggagcattcg gtgtggcggt gttgtcctcg atcttgagtg
    11041 cgtcgaatgt cgaagcagcg accgagaaat ttctttcggc gatcgagcgc gaatcatgaa
    11101 gaggattgct gcagagggcg tctcacgcat cgacacacgt cacgctgcat cggcgtctgc
    11161 tgctgttgac caccccatag gcgggtgata gaatccgaaa cagtgttgat gaccgttact
    11221 gacagggatt gtaagcgatc caacgagtgg ggcgatggcc gaatcaaaag ggagttcagg
    11281 ccgactccgg tccttccgtg attccgtcaa gcgcctgacc aagtcacttt ctgacggaga
    11341 tggagtcgtg acacacaaga acgacgaaca ggcccgcgag gtagaaaaac ttcgcatcca
    11401 aatccagtcc atggaggagg agattcggcg gctctatcag tcacgctacc agctcgaaca
    11461 aacgacgaag cagaacgaaa agctcgtcgc cactctccag gaagccaaat ctcaaatcga
    11521 aacattgcgg gctgaagtgg agaagttgac cgctccgccc tcgacctacg gcattttctc
    11581 cagcctcgat accgatggca cggggaatgt ctacgtatcc ggtcgtaaga tgaaggtcag
    11641 tcttcatccc tccatcaaag cgaagtctct ccgcaaaggg caggaggtca tcctcaacga
    11701 agcgctcaat gtcatcgagg tgcgtgggtt cgatgtgcaa ggggaagtcg tccgtttgaa
    11761 ggatgtgctg gaggggaatc gggcgctggt gacgctgcac ttcgatgaag aaaaagtcgc
    11821 ggagcttggc gagccattgc tgagtgagcg cctcagcgtc ggcgatcatc tgttatacga
    11881 tccgcggtcc ggttgcgtta tcgaaaagct gccgaaatcg gaagccgagg agctggtact
    11941 cgaagaagtc cccgatgtgg actacgaaca tatcggcgga ctccagaaag aaatcgagca
    12001 ggtgcgtgat gcggtggagt tgcctttcct gtatccgcac atttttgcga actacaaact
    12061 cagcgccccg aagggcgtgt tgctctatgg cccgccgggt tgcggcaaga cgttgatcgc
    12121 caaggccgtg gccagttcga tcgccaaaaa actggggcat ctcaaggaca aacaactgcg
    12181 cagctacttc ctccatgtga aggggcctga actgttgaat aagtatgtcg gcgagtcgga
    12241 gcggcaggtg cgcgaggtgt tcaaaaaagc caaggaacgc gccgacgacg gtcatcccgt
    12301 gattgtgttt ttcgacgaga tggacgcact gttccgcacc cgcggcaccg gcatctcctc
    12361 ggacattgaa tcgaccatcg tgccgcagtt cctctcggag atcgacggcg tagagcgtct
    12421 taccaatgtc atcgtcatcg gggcaagcaa ccgacaggac ttgatcgatc cggccgtgct
    12481 ccgcgcgggc cgtctcgatg tgaaggtgaa ggtcggtcgt ccggacgcca cggcggcccg
    12541 ggatattttc tccaagtacg tctcgaccga tttgccgttt gccgaggacg atctgaagcg
    12601 gcatggcggg gatgcccggg cacttgtgga ttccctcatg aacatgaccg tcgatgccat
    12661 gtatgcgacg acggacgaaa acaagttcat tgaagtgacg tatgcgaacg gcgagaagga
    12721 agtgttgtac ttcaaggatt tcgccagcgg cgcgttgatc gagggcatcg tgtcgcgtgc
    12781 caagaaattc gccgtgaagc gggcgattgc gcaagaagga accggtctgc gggccgagga
    12841 tctgatccgc gcgatccgag aggagttcaa agagcacgaa gacttgccca acaccacgaa
    12901 tccggatgat tgggcaaagg tcgccggcaa gaaaggcgag aagattgtcc acctccggac
    12961 gatcagcggc gggcctgcag aggcgcgcca aatcgaaaca gtcaacaccg gtcactac
//
LOCUS       DAORQN010000035        12813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_299_length_12813_cov_18.902728, whole genome shotgun sequence.
ACCESSION   DAORQN010000035 DAORQN010000000
VERSION     DAORQN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_299_length_12813_cov_18.902728"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK614_03865"
     CDS             <1..222
                     /locus_tag="PK614_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81375.1"
                     /translation="VLLPFFKERRMMLVDFKLGIIDPTKVSRCALQNAASVAGLMLTT
                     EVMITELPEEKKEAAGGHAGHNHGMEGMY"
     gene            complement(219..2033)
                     /locus_tag="PK614_03870"
     CDS             complement(219..2033)
                     /locus_tag="PK614_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81376.1"
                     /translation="MTRRSPQHDPPAPRRDSVSSRTFPDPSPLLVASGLEPEQVVKIL
                     SPYGITHPAEADTNIQSMAGDPHTRRILATILPGLLTEIARTADPDQALNHWERLFSG
                     SVNRSSLLQYLQASPKMLGLLCTIFGNSDSLAFALIRDPMLVYWLAEEDVLTTAPTRE
                     GMVAALRQSLGSLKAIELRLDVLRRVRRREMLRIGVRDLLHLATVEDTTGSLSDLASV
                     LIHGAYEIVDQDLKAVHGIPMHRDANGAWIETEFVVIGMGKLGAHELNYSSDVDLIYV
                     YASADGETRKGKGGRQSNSHGLSNEEYFELLARSLTKALAEQTREGAVFRVDLRLRAE
                     GSVGQLARSVEAYEKYYAHRGQVWERLALLKAWPIAGSPTVGQAFVRMVEGFVLQPDL
                     QPLSRERALAIMRDVRSVKEMIDEKIAGRGHQHRNVKLGTGGIRELEFLVQTIQVLIG
                     KAVPEILDRRTVGALHRFCQHRFVDADDQRRLTEAYWFLRDVEHKLQMMHDLQTHALP
                     DSDEELQRCAIRMGYSAESREGALAKFLADRQRHTAIVHQTFRSLFYHPETSALFAAV
                     VAAAEGHSRRSASRLEKETGPAGPPPDRSSTAKKKRKL"
     gene            2104..4380
                     /locus_tag="PK614_03875"
     CDS             2104..4380
                     /locus_tag="PK614_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81377.1"
                     /translation="MLHANADRDQILTTLAPLCQGIDRDILQDFVTRMDPDYFSAFSP
                     KTLAAHVKQAATLTPDHPCDVAIAETAGGHFVITIVAYDYFSEFATICGLLSAFGLNI
                     EEGRIFTSAESEQPSRSRSADPYPIRTRPQGRPGLTRKKIVDVFTVSPIEAQTFAAAD
                     RKRLTDQLARMIMLLDEGQLDEARQQVNRQLVEHLGKRRSSFSGLLHTVQITFDNSQS
                     ATDTIMDIRSDDTPAFLYAFANALAMRNVYISKALFAIEDGKLHDRFYIRNRFGQKLL
                     DPGDLEQLRLTAVLIKQFTHALTWAPDPAKALEAFDQFLDLVLEGSRQAGRKQAWAFV
                     KDKKTFPLLARLLGASDFLWEDFLRRQHVNLLPLLKDYRDAPLIKSQATLRKELNRAI
                     VKAKTDEARKEALNRFKDQELFRIDMKHIVEPGTSLPDFSLAISELAEVIVERSLVDC
                     QAKLTKLYGSPRLPNRKPCPFAILGAGKFGGKEMGYASDIEVLFVYGGPGRTSGKQGI
                     ENSEYFERLAQEFLQWIEAKQEGIFHIDVRLRPHGGKGSLANAFDEVCKYYSAEGQAA
                     PFERQALIKLRHIAGDAALGKKVETHRDSFVYSGAPWDLTVALDLRRQQVKQLVEPGQ
                     INLKHSHGGIVTLEYAIQYLQVMHGHRHPSLRTPNTLRALAALIDVGLIPRATGENLR
                     KSYLFIRMLIDGLRMVRGNTKDLVIPPPDSDEFIFLARRVGYQTEDWQAGARHLQTDI
                     EEHMKQNRQFFEKMFGKV"
     gene            4846..5946
                     /locus_tag="PK614_03880"
     CDS             4846..5946
                     /locus_tag="PK614_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB81378.1"
                     /translation="MAYNWEQPDWPNFRYDLSGLQDSLLLLAERTGRASGLLQGLTPD
                     AQVEATVMIMVTEALKTSAIEGELLSRKDVMSSIRKNLGLETGFPSGDEHAQGAAALM
                     LAVRNTIETPLSKDLLFAWHRTVMAGRRHVATGQWRTDAEPMQVVAGAYGHEKVHFEA
                     PPSSRVPSEMARFIRWFNETAPGGRKAIQKAAVRSAVAHLYFESIHPFEDGNGRIGRA
                     LSEKALSQGLGRPALLSLSNAIETKRRDYYDALKEAQQSNEITPWVTWFIHMTLEAQV
                     QAEAQIDFTLKKARLFDRHRNELNERQLHILTRMLEEGPGGFEGGMSAKKYMSITGAS
                     KATATRDLQELTIKGIFEPIGGGRSTGYKINL"
     gene            6180..7007
                     /locus_tag="PK614_03885"
     CDS             6180..7007
                     /locus_tag="PK614_03885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HRB81379.1"
                     /translation="MKDIPQRVMDEVMNSLKNYLVAFYILIPQRQQSRIRMIGSGTLV
                     ELERDHYILTAAHVWHETREADKVGLVLTDHQSSFLIPRDSLQAKEVWSGRLTEWGPD
                     VALLKIPSPFIPTISAHKSFLNLALQKQNLRISPPTIQNGLWAVTGMVGELSEVQDHP
                     ETGTTEGHVDGEAFFSVVQDTHEHNGFDYFDLGANLSLSGVPSSFGGVSGGGLWEIKL
                     SMSESTNEIRWDEKRHLRGVAYWQSEGSTNHRVIRCHGPKSIYETAWESWSLSSGED"
     gene            7278..7574
                     /locus_tag="PK614_03890"
     CDS             7278..7574
                     /locus_tag="PK614_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRB81380.1"
                     /translation="MAKTLSLSEVKTRLPELVAGVQEREEEVIVTKNGRPAAILINVD
                     EYTRLKETVDVLSDPGLMSQIAESRAFYKAKHKGLSFEDVFGEPLTPIKKRRTA"
     gene            7571..7864
                     /locus_tag="PK614_03895"
     CDS             7571..7864
                     /locus_tag="PK614_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB81381.1"
                     /translation="MTPFRPDIPPHVAEVIRTLHPDLKQLIKSAIRAIAANPECGEPL
                     QRELDGLRKYRVRRFRIVYAVEQKRRVIRLMAVGHRRYVYEELTDRLRRKTRE"
     gene            8025..8708
                     /locus_tag="PK614_03900"
     CDS             8025..8708
                     /locus_tag="PK614_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRB81382.1"
                     /translation="MPKKILVVLTATEKYPNLNRATGVWLGEAVHFVEKVERAGFAVD
                     YMTPSGGYTPIDPHSLAMAEPIDWEWYHNRQFMNRLGATLKPSDVNPGDYAAIYFVGG
                     HGVLWDFPDNADFQQLSRKIYENGGIVSSVCHGAVGLLNITLSDGTLLIKGKQVTGFS
                     DEEERLAQLDQYVPFLTEAELKKRGAAYRKAEKPWEAFAVADHRVITGQNPASGGAVA
                     DLVIKALKG"
     gene            8858..9292
                     /locus_tag="PK614_03905"
     CDS             8858..9292
                     /locus_tag="PK614_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81383.1"
                     /translation="MMLRRLSVILLCCAGLTACGESGDSAGSGLKPVGEWAKIGETDN
                     YVYYADYASIKKADETVVMLDLFDYKSVQTEGGNAPALSKATQREYDCQNKKSQSLKS
                     SWFSGQMGAGNVVRSAGTSNQWSVATQGTATGGLLKAACGNS"
     gene            complement(9385..10161)
                     /locus_tag="PK614_03910"
     CDS             complement(9385..10161)
                     /locus_tag="PK614_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB81384.1"
                     /translation="MAKRVKSSQEPADLLSRHIEQIQATLVAIQPFLSRRKATASLDG
                     FDEQTEEVLSETFGGASEELEAYQYAKLGEAAILPEEAQEAGTHNVDRESLHQRKQVL
                     ESCLAQLELRKATRAGRDWRRTLGERIDGHTVAEFMSAEVRSVHKSASIKEAGRLLQK
                     WRIGSLLVDDGSRYIGIITDTDLSRKAVAKGLDPNTTTVLSCMSKSVVTIEDSEPLME
                     ALRLMKKEGIRHLPVTEDGTIIGVLSVGDLLRAYQKVLEL"
     gene            complement(10266..11861)
                     /locus_tag="PK614_03915"
     CDS             complement(10266..11861)
                     /locus_tag="PK614_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HRB81385.1"
                     /translation="MNKIEAIKSERDGLTVRDMIAHYAQAGWEAIPEDDIQRLKWYGL
                     FLRNPTPGHFMLRVRLPGGQTTSAQLETLADIAQQYGNGVVDVTTRQQVQLRHLTIAH
                     VPSVFSKLEEAGLTSLQTGMDTVRNIMTCPVAGLNPNELLDGTDIVRAINHEVLGNPA
                     YTNLPRKCNIAVTGCPDNCLHTETQDIALVPAYHDLGHDKCYGYNVLVGGKLGSGGYR
                     IAGSLDMFVNPAEAFEVCRTLLHIYRDHGPRENRTQARLAFLVEAWGEARLRHEVERL
                     VGRTLPTAGVDARGAAERDHIGIFRQKQRGMNYVGLKVLVGRVKAADLRSIAALAVRY
                     GNGEVRLSPAQAFVIPHISDRLVGELAEEPLVKQFAYNPSALYKGLVSCVGSDYCNLA
                     VIETKSRAVETARVLERKLGDTLKPITLHWSGCPAGCGNHLVADIGLLGKKAKVGGKV
                     VDAVDVFVGGRSGPDPKLATKIMEDVPCDRLPAVLEMIMPYHTREKMHRVRGKAVPKA
                     TGASKSTASGNETTGLTPQPLPA"
     gene            complement(11861..12055)
                     /locus_tag="PK614_03920"
     CDS             complement(11861..12055)
                     /locus_tag="PK614_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81386.1"
                     /translation="MSTNAETTVLTVLEHQAPQTFEQLMALSGLSASQVLLVVDQLSR
                     AGAVVLRKSGPDYSVALGGT"
     gene            complement(12107..12547)
                     /gene="cynS"
                     /locus_tag="PK614_03925"
     CDS             complement(12107..12547)
                     /gene="cynS"
                     /locus_tag="PK614_03925"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:TIGR00673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HRB81387.1"
                     /translation="MEKAAVRKAIKAQRLNKKVTIAEVAKTVGKNPTFVAAALNGNHR
                     LSPEEAGKVGKLLDLDKEQISSLSAFPVRADFPNATDPFKYRLLEVIGVYGDSMREMA
                     NEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(12601..>12813)
                     /locus_tag="PK614_03930"
     CDS             complement(12601..>12813)
                     /locus_tag="PK614_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="member of the FNT family of formate and nitrite
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HRB81388.1"
                     /translation="SLLGMALFLPHDAAVSWGAFWYNQLFVVLGNLVGGGLFVGGLYW
                     MVSPYRVTEATAAPVAQPVVSAVAGS"
BASE COUNT         2788 a         3984 c         3498 g         2543 t
ORIGIN      
        1 gtgttgctgc cgtttttcaa agaacgccgg atgatgctcg tcgatttcaa gttgggcatc
       61 atcgatccga caaaggtgtc gcgttgcgcc ttgcagaacg ccgccagcgt cgcgggcttg
      121 atgctcacga ccgaagtcat gatcacggag ttgccggaag agaagaaaga agctgccggt
      181 ggccatgctg gtcacaacca cggcatggaa ggtatgtact aaagtttccg tttcttcttc
      241 gctgtcgaag accggtccgg cggtggcccc gccgggccgg tctctttttc gagccgagac
      301 gcgctgcggc gggagtggcc ttccgccgca gccactacgg cggcaaacag ggcggaagtc
      361 tccgggtggt agaagagaga ccggaacgtc tgatgtacga ttgccgtgtg ccgctgccgg
      421 tccgctagaa acttggccaa cgccccctcg cgcgactccg cgctatatcc catccgaatc
      481 gcacaacgtt gcagttcctc atcgctgtca ggcaacgcgt gcgtctgcaa atcatgcatc
      541 atctgcaact tatgttcgac atcccgtaag aaccaatagg cctcggtcag tcgacgctga
      601 tcgtctgcat ccacgaaccg gtgttggcaa aaccggtgca gggcacccac cgttctgcga
      661 tccagaatct cgggaaccgc cttgccgatg agcacctgaa tcgtttggac gaggaattcc
      721 agttctcgaa tcccccccgt gcccaacttc acattccgat gctgatgtcc gcgccccgcg
      781 atcttttcat cgatcatctc tttgacggaa cggacatcgc gcataattgc cagcgcccgc
      841 tccctcgaca ggggctggag atcaggctgc aaaacaaatc cttcgaccat ccgcacgaac
      901 gcctgtccga ccgtaggaga accggcgata ggccaggcct tgagcaacgc cagccgctcc
      961 cacacctggc cacggtgggc atagtacttt tcgtaagctt cgaccgatcg tgcgagttgg
     1021 ccgaccgaac cctctgcgcg cagccgcaaa tcaacgcgaa acaccgcacc ttcacgagtc
     1081 tgttcggcca gggccttcgt cagtgaacgc gccagcagct caaaatattc ttcattggac
     1141 aggccatgac tgttcgattg gcgcccgccc tttcccttcc tcgtctcacc atctgcggag
     1201 gcatagacat aaatcagatc cacgtccgag ctgtaattca gctcatgcgc gccgagtttg
     1261 cccatcccga tgacgacaaa ctccgtctcg atccaggcgc cgtttgcgtc tcgatgcatc
     1321 gggatcccat ggaccgcctt aaggtcctga tcgacgattt cataggctcc atggatgagg
     1381 accgaggcga gatcggacaa ggagccggtc gtgtcttcga ccgtggccag gtgcagcagg
     1441 tcccgcacac cgatgcgcag catttcccgg cgacgcaccc gccgcaggac atccagcctt
     1501 aattcgatcg ccttcagcga gcccaggctt tgacgaaggg ccgccaccat tccttcccgg
     1561 gtcggagcgg tcgtcaagac atcttcttcc gccagccagt acacgagcat ggggtcgcga
     1621 atgagagcga aggcaagaga atcgctgttt ccaaaaatcg tacagagcag ccccaacatc
     1681 ttcggcgagg cttgcagata ctgcaggagc gaggagcgat tgacactccc tgaaaagagt
     1741 cgctcccaat gattcagggc ttgatcagga tcggccgtgc gggcaatctc tgtcaacagc
     1801 ccaggcagaa tcgtggccag aatccgacgt gtatggggat cccccgccat actctggata
     1861 ttcgtatccg cctcggccgg atgtgtgatc ccgtaggggc tcagaatctt gaccacctgc
     1921 tccggctcaa gcccggacgc caccagcaag ggggatggat cggggaaggt ccgggaggaa
     1981 acggaatccc ggcgcggggc aggcgggtcg tgctgtggcg aacgtctggt catggcgccg
     2041 cattatactg gcgcaattct tccggtacaa cgagaccgcc ttcgggtata ctccaccgga
     2101 atcatgctgc acgcaaacgc agaccgcgac cagatcctca caaccctggc tccgctctgt
     2161 cagggcatcg accgcgacat cctgcaagac ttcgtcacga ggatggaccc ggattatttc
     2221 tccgcctttt cgccgaagac actcgccgca cacgtcaagc aagccgccac gctcacaccg
     2281 gaccacccct gtgacgtggc gattgcagaa accgcaggag gccacttcgt catcaccatc
     2341 gtggcctacg attacttttc cgaattcgcc acgatctgcg gcctcctatc tgccttcggt
     2401 ctcaacatcg aggaaggtcg catcttcacc tcagcagaaa gtgaacaacc cagccgcagc
     2461 cgatcggccg atccgtaccc gatcaggaca aggccgcaag gtcgccccgg gcttactcgg
     2521 aagaagatcg tggacgtgtt cacggtgagc cccatcgagg cgcagacgtt cgccgccgca
     2581 gatcgcaaac gcctgaccga ccaactcgcc cgcatgatca tgctgctcga cgaagggcaa
     2641 ctggacgaag cccggcaaca ggtcaaccgc caattggtcg aacacctggg caaacgccgg
     2701 agttccttca gcggtctgct ccacacggtc cagatcacct tcgacaacag ccagtcggcg
     2761 accgatacga tcatggacat ccggtccgac gacacgccgg cgtttctcta cgcgttcgcg
     2821 aacgcgctcg caatgcgcaa cgtctacatc agcaaagcgc tgttcgcgat cgaagacggc
     2881 aagctgcacg accgttttta catccgcaac cgcttcggac agaagctgct cgacccgggc
     2941 gacctggagc agctgcgcct caccgccgtg ctcatcaaac agttcaccca tgccctcacc
     3001 tgggcgcccg atccggccaa ggcgctggaa gccttcgacc agttccttga cctcgtactc
     3061 gaaggctcgc gtcaagccgg acgaaaacag gcctgggcct tcgtgaaaga caagaaaacc
     3121 tttccgctcc tggcacggct gctcggggcg agtgattttc tgtgggaaga cttcctccgg
     3181 cggcaacacg tcaacttgct gccgttgctg aaagattatc gcgatgcgcc gctcatcaaa
     3241 tcccaggcga cgctgcgcaa ggaattgaac cgcgccatcg tcaaggcgaa gaccgacgag
     3301 gcacggaagg aagccctcaa ccgattcaag gatcaggaac tcttccgcat cgacatgaaa
     3361 catatcgtcg aaccggggac gagcctgccc gacttctcgc tcgccatctc agaactggct
     3421 gaagtgatcg tcgaacgcag cctcgtggac tgccaggcca aactgacgaa gctctatggc
     3481 agcccccgtt tgcccaacag aaagccctgt ccctttgcga tcctgggcgc ggggaaattc
     3541 ggcggcaagg aaatgggcta cgcatccgac atcgaggtgc tcttcgtcta cggcgggccc
     3601 ggacgcacaa gcggcaaaca ggggatcgag aacagcgaat acttcgaacg ccttgcgcag
     3661 gaatttttgc aatggatcga agccaagcag gaaggcattt ttcacatcga cgtccgactg
     3721 cggccccacg gcggcaaggg gtccctcgcc aacgcgttcg atgaggtctg caagtattac
     3781 agcgcagaag gccaggctgc tccgttcgag cggcaggcgc tcatcaagct gcgccatatc
     3841 gcgggagacg ccgcgctcgg caagaaggtg gagactcatc gcgacagctt cgtttacagc
     3901 ggcgcgccct gggatctgac ggtggcgctc gacctgcggc ggcaacaggt caaacaactc
     3961 gtcgagccgg gccagatcaa cctcaagcac agccacggcg gcatcgtcac gctggaatac
     4021 gccatccagt acctgcaggt catgcacggc caccggcacc cgagcctgcg cacgccgaac
     4081 accctccggg ccttagccgc cctgatcgac gtagggctca ttccccgcgc gaccggcgag
     4141 aacctgcgga agtcctatct cttcatccgc atgctgatcg acggcctgcg catggtgcgc
     4201 ggcaacacca aggacctcgt catcccgccg ccagactccg acgaattcat cttcctcgcc
     4261 cgccgcgtcg gctaccagac cgaagattgg caggccgggg caagacatct acagacagat
     4321 atcgaagagc acatgaagca aaacagacag ttcttcgaga agatgtttgg gaaggtgtga
     4381 tggaaaggga agaggatgga ggaggacagg ccttcgcggc tccggcgatg gctcaagcat
     4441 gtagggcctt gtggtccctg ggctgtgcaa ctcgttccat tccggattct atccgaatgg
     4501 aacttcgaac agtgctcacc ctcgcctccg gcgaccgcgc ccccaaggcc ctgcgagcca
     4561 tcgcaagtcg ccgctctcac ctgtcctcct ccaactcaga gagagtgggc agcagcaagc
     4621 ccagcaggac cattgatatc aactcgcaat cggggagcag tcaagcccgc tcccatgaag
     4681 ggaaaccggc aagattagaa gcaatatgta gctttggagc cctctaccgg tcgctgttct
     4741 cactccacac cctaaaacca gccagaggat agatgagccg atcagagaac gtattcggct
     4801 catatttgac ttaatacatg agccgattat ctgtataatc ggctcatggc ctataactgg
     4861 gaacagcctg actggcccaa cttccgctac gacctctccg gtctccaaga cagcctcctt
     4921 ctactcgccg aaagaaccgg ccgtgcgagt gggctcctgc aggggctgac ccctgacgcg
     4981 caggtcgaag ccaccgtcat gatcatggtg accgaagcgc tcaagacctc ggcgattgaa
     5041 ggtgagctcc tcagccgcaa ggacgtcatg tcttcgatcc gaaagaacct tggcttggag
     5101 acaggcttcc ccagcggaga cgagcacgca caaggcgccg ccgcgctgat gctggccgtc
     5161 cggaatacga ttgagacgcc cctctcgaaa gacctactct ttgcctggca tcgcacagtg
     5221 atggcaggac gccggcatgt ggccacggga caatggcgga ccgatgcgga gccaatgcaa
     5281 gtcgtggcgg gtgcatacgg gcatgaaaaa gtccatttcg aggccccgcc ttcctctcgc
     5341 gttccaagcg agatggcgcg gtttatccgc tggttcaacg agacggctcc cggtgggaga
     5401 aaagccattc agaaagctgc ggtgcgctca gccgtcgcac acctgtattt cgaatcgatc
     5461 catcctttcg aggacggcaa tggtcggatc ggcagagccc tttccgagaa ggccctatcg
     5521 cagggactcg gtcgccccgc cttgctgagc ctttcaaacg cgatcgaaac caaacgtcgc
     5581 gactattatg acgcgttgaa agaggcccaa cagtcgaatg agattacgcc gtgggtgacc
     5641 tggttcatcc acatgacgct tgaggcgcag gttcaggcag aagcgcaaat tgactttacc
     5701 ctgaagaaag cacggctgtt cgaccggcac cgcaacgaac tcaacgagcg acagttgcac
     5761 atcctgaccc gcatgctgga agaggggcca ggcggcttcg agggcggaat gagcgccaag
     5821 aaatatatgt cgatcaccgg cgcctccaag gccacggcaa cacgcgacct ccaggaactg
     5881 acgataaagg ggatcttcga accgatcggt ggcgggcgga gcacggggta taagatcaat
     5941 ctataaaagc agcaaccggt ggtagctaga gttggcacgt caccgcggca atgactggca
     6001 agccggtgcc aggaatccgc agacggatat tgcgcatcac atggccacca aagagctctt
     6061 cgaaagaagt tttcgcaagc gtacgactta tttacttaat gttcgactgc aagacctgac
     6121 acattagttt ccacaccttt tggataatta cggagagcaa attgaggaac agcatcacga
     6181 tgaaagatat tccccaacgc gtgatggacg aggtgatgaa ttccctcaag aactacctcg
     6241 ttgcattcta tattttgatt ccgcagaggc aacaatctcg tatcaggatg atagggtccg
     6301 gaaccctggt ggagcttgaa cgggatcact acattctgac tgctgctcat gtctggcatg
     6361 aaacgagaga ggcggataag gtcggcctag ttctcaccga ccatcaatca tctttcctga
     6421 taccacgtga ttctcttcag gcaaaagagg tctggagcgg cagattgaca gaatggggcc
     6481 cagacgtggc tcttctgaaa atcccgtcgc cgtttattcc cacaatatct gcacataagt
     6541 cttttctaaa cctagcctta cagaaacaga accttcgaat ctctcccccc acgatacaga
     6601 acggtctttg ggctgttaca ggcatggtcg gcgagttgag cgaggttcag gaccatccag
     6661 agacaggaac aactgagggt catgtcgatg gcgaagcatt cttttctgtc gtgcaagaca
     6721 cacatgaaca caacggtttt gactatttcg accttggagc taatttgagc ttgtctggtg
     6781 ttccatcgag cttcggtggc gttagcggag gtgggctctg ggaaatcaag ctttcaatgt
     6841 cagaatcaac gaatgaaata cgttgggacg agaaacggca cttgcgtggg gtcgcatatt
     6901 ggcaatcgga aggctcaaca aatcatcggg taatccgctg tcacggtccc aagagtatat
     6961 atgaaacagc atgggagtca tggtcacttt cttcaggaga agactgaatg gatgtcggga
     7021 tctatgcgag caagccgact cagacgctcg accgacgcgt ggctacggca ctttccactt
     7081 tggaatatgc acgagactga agaactactg cctcctggtg ttctacagct tccgtcatgc
     7141 ctctcagttc gtaagcttga tattccccct caccgctcac gtcgcacagc gaccatttgc
     7201 ccgttcattt gacggagcct tacctgtggt tgtatcctaa cgaccagaat tctggtctct
     7261 tatacacgag gtctaccatg gcaaaaacat tgtcgttgtc ggaggtcaaa acgcgcctgc
     7321 ctgaattggt ggctggggta caagaacgcg aggaggaagt cattgtcacg aagaacggcc
     7381 ggccggctgc cattttgatc aatgtcgatg aatatacccg cctcaaagag accgtggatg
     7441 tcctgagcga tcctggactc atgagccaga tcgccgagag ccgggccttt tacaaagcca
     7501 aacacaaagg gctctcgttt gaagacgtgt tcggcgaacc cctcacgccc atcaagaagc
     7561 gtcgcacggc gtgacgccat tcagaccgga catccctccg catgtcgcag aggtgatccg
     7621 tacactccat cccgatctga agcaactgat caagtcagcc attcgtgcca ttgccgcgaa
     7681 tcccgaatgc ggggagccgc tccaacggga actggacgga ttacgaaaat atcgcgttcg
     7741 ccgctttcgc atcgtctatg ccgttgagca gaaaagacga gtcatccgcc tgatggccgt
     7801 cggccaccgt cgctatgtgt atgaagagct gaccgaccgg cttcgacgca agacccgtga
     7861 atagcactac gccggtcgcc ccccttaccc tctcgcttct acctcctctt actaaggctc
     7921 cacaggaagg cggtttctga tctttcgcgc acacgtcctt gtgcacgctc gtgacattgt
     7981 gccctaatat cctgaaccgc tcctgaaaac cgaaaggact ccccatgccc aagaaaatac
     8041 tcgtggtgct gacggcgacg gagaagtatc cgaatctgaa ccgggcaacc ggcgtgtggc
     8101 ttggcgaagc cgtgcacttc gtggagaagg tcgaacgggc tggatttgcc gtggattaca
     8161 tgactccgtc aggcgggtat acgccgatcg atccccacag cctcgccatg gcggaaccga
     8221 ttgactggga gtggtaccac aatcgccagt tcatgaaccg tctcggcgca acgctcaaac
     8281 cgagcgacgt gaaccccggt gactatgccg ccatctattt cgtaggcggg catggcgtac
     8341 tctgggactt tcccgacaat gccgattttc agcagctcag ccgcaagatc tacgagaacg
     8401 gcggaatcgt ctcatcggtc tgtcacggcg ccgtcggctt attgaacatc accctgtcgg
     8461 acgggacgct gctgatcaaa ggcaaacagg tcaccggatt ctcggatgag gaagaacgcc
     8521 tggcccaact cgatcaatac gtgccgttcc tgaccgaggc cgaactgaag aaacgaggcg
     8581 ccgcttatag aaaggcggag aagccctggg aagcattcgc cgttgcagat catcgcgtga
     8641 tcacgggaca gaaccctgcc tccggcggcg ccgtggccga cttggtcatc aaggccctca
     8701 agggctgata acaaccagac tacaccatcg gcaggtgccg tcagacttgc gcgatcacga
     8761 agtccccacc catgcctggc ggttaactcc gccaggtccc cgcgtcgaga ttcggcaaaa
     8821 ccatgttaca gtgggaactg cggagtgatt ggagtggatg atgctacgac gactatctgt
     8881 gatcctgctc tgctgcgcgg gtctgactgc atgcggagaa agcggtgaca gcgccggctc
     8941 cggcctcaag ccggtgggag aatgggcgaa gatcggcgag acggacaact acgtgtatta
     9001 cgccgactac gccagcatca agaaggccga tgagaccgtc gtgatgctgg acctgttcga
     9061 ttacaagagc gtacagaccg aaggtggaaa cgcccccgct ctttccaaag ccacacaacg
     9121 cgaatacgac tgccaaaaca agaaaagcca gtcgctcaag tcgagctggt tttcaggaca
     9181 aatgggagcc ggaaacgtcg tgcgctccgc cggcacgtcg aaccaatggt ccgtggccac
     9241 gcagggcacc gccacaggcg ggctcttgaa ggccgcctgc ggcaattcct agaatcgcgg
     9301 tcgattcatg aaaaccggac tggccgcatt tccggaggag cttctttacc cgacaagtct
     9361 actctggtct ccggattgcc ttccctacag ctccagcacc ttttgatagg cccggagcaa
     9421 gtcacccacg gagagcactc cgatgatcgt gccgtcctcc gtgaccggca gatgtcggat
     9481 gccctctttc ttcatcaatc tgagcgcttc catcagcggc tcactgtcct cgatggtgac
     9541 cacggatttg ctcatgcagg agagcaccgt ggtcgtgttc gggtccagtc ccttagcgac
     9601 ggccttccgg ctgagatccg tatccgtgat gatgccgatg tagcgcgatc cgtcgtccac
     9661 cagcagggaa ccgatccgcc acttctgcag caaacggccg gcctccttga tcgacgcact
     9721 cttatggacg ctcctgactt cggccgacat gaactcggcc accgtgtgac cgtcgatccg
     9781 ctcaccgagg gtcctgcgcc aatcccgacc ggctctggtg gcttttctca actcaagctg
     9841 ggccagacaa ctttccaaca cctgcttccg ttgatgaagg ctttcgcgat ccacgttgtg
     9901 cgtgccggcc tcctgcgctt cctccggcag gatcgcggcc tcgcccagct tggcgtactg
     9961 ataggcttcg agttcctcgg acgctccgcc gaaggtttca ctcagcacct cttcagtctg
    10021 ctcatcgaat ccgtccagcg aggcggtggc cttacgtcgg gataagaatg gctggatcgc
    10081 caccaacgtc gcttgaatct gctctatgtg ccggctgaga agatccgccg gttcctgact
    10141 cgatttcact ctcttggcca tgctgcctcc ttgttgaggg caagagaata acgcaactgt
    10201 ggcagcggct caagccttcg cgcctggcag gctgctgaaa aagtccgtcg gctttgaagg
    10261 gccctttagg caggaagcgg ttggggcgtg agccccgtcg tctcgttgcc ggatgccgtc
    10321 gactttgagg ctcccgtggc ttttggcacg gccttcccgc gcacacgatg cattttttcg
    10381 cgcgtgtggt agggcatgat catttccaac accgctggaa gccgatcgca gggcacatcc
    10441 tccatgattt tggtggcgag cttgggatcg gggcccgagc gcccgcccac gaacacatcc
    10501 acggcatcga cgaccttgcc gcccaccttc gccttcttgc ccaagagtcc gatatccgcc
    10561 acgagatggt ttccacaccc ggccggacaa ccggaccaat gcaaggtgat cggcttgagg
    10621 gtatcgccca gcttccgctc cagcacccgc gccgtttcga cggcccggct tttggtttca
    10681 atgacggcca gattgcagta gtcgcttccc acacaactga ccagtccctt gtagagggcg
    10741 gacgggttgt aggcgaattg cttgaccagc ggctcttccg ccagttcacc caccagccgg
    10801 tcgctgatat gagggatcac gaaggcttgc gccggcgaga gacgtacttc tccattgccg
    10861 taccgaaccg ccaacgcagc gatgctccgg agatcagccg ccttcacccg accgaccagc
    10921 accttcagcc cgacatagtt catgccccgt tgtttttgac ggaagatgcc gatgtggtcg
    10981 cgctccgccg ctcccctcgc atccaccccc gccgtgggca gggttctccc gaccagccgt
    11041 tcgacttcgt gccgcaaccg agcttcgccc caggcctcca cgagaaatgc gaggcgcgct
    11101 tgggtgcgat tctcacgcgg tccatgatcg cgatagatgt gcagcagcgt ccggcaaacc
    11161 tcgaaggctt ccgccggatt cacgaacatg tccagggagc cggcaatgcg atagccgccg
    11221 gaccccaact tgcccccgac cagcacgttg tacccgtaac acttgtcatg ccccaggtcg
    11281 tgataggccg ggaccaacgc gatgtcttgc gtctccgtgt ggaggcaatt gtccggacaa
    11341 ccggtgaccg caatattgca cttgcgcggc agattcgtat aggccggatt acccagcacc
    11401 tcgtgattga tcgcgcgcac gatgtccgtc ccgtcgagca actcgttggg attcaggccg
    11461 gccacgggac aggtcatgat gttccgtacc gtgtccattc cggtttgcag tgacgtcagg
    11521 cccgcctcct ccaacttgct aaacaccgac ggcacatggg caatcgtcag atgccggagt
    11581 tggacttgtt gcctggtggt aacatcgacc accccgttgc cgtattgctg cgcgatgtcg
    11641 gccaacgtct cgagttgagc cgacgtggtt tggccacccg gcaggcgtac ccgcagcatg
    11701 aagtggcccg gagtcggatt gcgcaaaaat aacccgtacc acttcaaccg ttgaatatcg
    11761 tcttccggaa tagcctccca tcccgcttgc gcatagtgtg cgatcatgtc ccgcaccgtc
    11821 agcccgtccc gctccgactt gattgcctcg atcttattca tcacgtgcct cccaatgcga
    11881 cggaatagtc cggtccggat ttcctcaata ccaccgctcc tgcccgactc aactgatcca
    11941 cgaccagcaa cacctgtgac gcgctcaaac cggagagggc catcagttgc tcgaatgtct
    12001 gaggagcctg atgctccagg actgtcagta cggtagtttc tgcattcgtg ctcatgatgt
    12061 cctcctttcg gttgatcgcg atccacgcat cagggaccga ttcctcttaa aacgttttgt
    12121 actcaagcca cttgccgttc agcgtgatct tgcaccgggc ctcgccctga ctgccggtga
    12181 ccttttccat atccagcgta aagtcgatgg cgctcatgat cccgtcgccg aacatttcgt
    12241 tcgccatctc ccgcatcgaa tcgccgtaga caccgatgac ttccaacagg cgatacttaa
    12301 acggatccgt cgcattcggg aaatcggccc ggacgggaaa cgcgctcagg gagctgattt
    12361 gttctttatc caaatccagc agcttcccca ctttgcccgc ttcctccggt gataggcggt
    12421 gattcccgtt taacgctgcg gcgacgaacg tcggattctt cccgaccgtc ttcgccacct
    12481 ctgcaatcgt gaccttcttg ttcaaccgtt gtgccttgat cgccttgcgt actgctgctt
    12541 tttccattgt gatctccctt tagtgattcg cccgtgaata cactcccgca ccgcgcttca
    12601 ttacgatcct gccaccgccg acaccaccgg ttgagctacc ggggccgccg tcgcttcagt
    12661 cacgcgatag gggctcacca tccaatagag ccccccgaca aacaacccgc cgcccaccag
    12721 gttccccagc acgacgaaca gctggttgta ccaaaaggcc ccccaactca cagccgcgtc
    12781 atgcggcaga aacaacgcca tgcccaacaa cga
//
LOCUS       DAORQN010000036        11947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_315_length_11947_cov_13.391187, whole genome shotgun sequence.
ACCESSION   DAORQN010000036 DAORQN010000000
VERSION     DAORQN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_315_length_11947_cov_13.391187"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..473
                     /locus_tag="PK614_03935"
     CDS             21..473
                     /locus_tag="PK614_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81389.1"
                     /translation="MIVQLQAAFRSLLGPAFDGYSCGNAAARSDLQKITLKELVKNGW
                     PEETTPLIQTRVTYPENISKNNPDYKCRALLHAKVGERGYESFSFAVYYNYDDYKVTD
                     YKSDFTKYQRMKIADLLAGKLEPLAQKMKVCSQQEKAEGCEDGRDRLH"
     gene            451..1728
                     /locus_tag="PK614_03940"
     CDS             451..1728
                     /locus_tag="PK614_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB81390.1"
                     /translation="MAETDSINHLTHKGLLIIVMALLGISFSLGDGQTWSFSRSAFAE
                     EIVPSNDTSFEQTKPCSLWLSGTPRPATSTFSKPLGDTNANNKGPADALREYLAQVQA
                     RIRQSWSIIPINLPNRALTTIINFRLERAGRVSNVTIQQSSGNEYTDLAARQALQRAV
                     PLPPFPPYIADRYLDAAYTFFVEELKKTSDQPRSGLIRGHALAGTELIVTDQSASEES
                     WTSQRYQYGDCRKHLETLTQVIDEVADSQAGLSRLQHIANDLPFSENNTESFENSDIV
                     SLVESLRYRIKARSEEIKVKLVTDSEMERFDNVCKPEILKSGFPDRLRDEPVASSRPE
                     TKTLGRAFCTAINGGGKVEFHVIDEKTPTVGIFVTTKKRRFVVVLQKKMQGDLSEEWT
                     AIEIQTPSDRKPIINMLGYSFMDMRFPALVRNE"
     gene            1823..2587
                     /locus_tag="PK614_03945"
     CDS             1823..2587
                     /locus_tag="PK614_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81391.1"
                     /translation="MNNYSEGRLIGEYFKAEQQRLLCYMRENEAPQVCWPDGCYGSGL
                     PWLTFVGPSPGGGNGRQPEVARSLMANRPLWNEDYTDPCQLWSPGFRTSIRMLIEIIL
                     GRSSEFGALRLYNFLNFDWMQNPKASEVPRERMRRGAEVVLDHLESSRPRTVVTLESS
                     SHSLLKEMLSRKYQLRPAEFGDVSILSYKTHAHRAMDAFEVLGDTGLGGSFVLRCPQH
                     PARIFRQDYAGRVGRALRHTLVSLAEAQRPISIYEQ"
     gene            2851..3081
                     /locus_tag="PK614_03950"
     CDS             2851..3081
                     /locus_tag="PK614_03950"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HRB81392.1"
                     /translation="MKTTMMQFRVNEEEKALIEKCAKKGGMTVSEYIRACMLMEMVID
                     GEVAALRIIGRTIGMKAMDAMKRRLARNPVTE"
     gene            3215..4222
                     /locus_tag="PK614_03955"
     CDS             3215..4222
                     /locus_tag="PK614_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB81393.1"
                     /translation="MDSAFTLSIDWLAFTVLASRPQETMKVLGGEWSKAKGGFRGYPL
                     SWIKLDGLHGVGKLGTNAPRRPNEIHVDLSGGLVSALTLDQVRALLKWVHEQQGHVTR
                     VDCALDDRAGTVSVATVREAVSAGQCVTRAAQVRHIASNLTHGTGASTGETLYFGSPQ
                     SQTLLRIYDKRLEMQSKERENWQDYGVRWELELKKDRAEQCARALASLNETDWKEFVV
                     GLLRSYVDFRQITKETEDEDRYRAPMLEWYALLTEGFQKGRLAQEKQVQTLQNVKRWV
                     SHALTPLLAVICATPGGEEWLQQEIVRGIARWKDRHRNLLKQPPNRFHTSAGGHAGSP
                     C"
     gene            4265..4576
                     /locus_tag="PK614_03960"
     CDS             4265..4576
                     /locus_tag="PK614_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81394.1"
                     /translation="MLTTKELSAWLNIKPSTLYLWASQNKIPCRRIHGLIRFDPDAIQ
                     AWLNSFGVTPGKPPSLPIRHVSRDLDQLIEAAKREVYTPRHGETIQSSLIRKEDADGA
                     I"
     gene            4596..5636
                     /locus_tag="PK614_03965"
     CDS             4596..5636
                     /locus_tag="PK614_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB81395.1"
                     /translation="MTFVHQGQQIRRSTGTTDRRLADAILGKVRVKIVEGRFFDTLEA
                     KDHTFNEMMDRYLIERSVTKAPASRLRDEQCLVHLRSAFGTLSLADVTPKLLAAYKAQ
                     RRERAKPATVNKELGLVRHSFNVAIREWEWCRDNPMQKVCMEKVRNERDRWLSSDEEG
                     RLLAHSAPWLQDIIVFALNTGMRRGEILNLQWQAVDLTRQVLVVMRSKNGEKRTVPLN
                     GRLTELLQRKAPKKTTGSFVFASSAETLVDARNLTRAFRLAAKKAELIDFRFHDLRHT
                     FATRLAQAGVDLYKVQRLLGHKTAAMTQRYAHHSPESLREGVLILERVHPQISQKYHN
                     EGATVGGCCASA"
     gene            complement(5643..5717)
                     /locus_tag="PK614_03970"
     tRNA            complement(5643..5717)
                     /locus_tag="PK614_03970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5683..5685),aa:Gly,seq:gcc)
     gene            6001..6330
                     /locus_tag="PK614_03975"
     CDS             6001..6330
                     /locus_tag="PK614_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81396.1"
                     /translation="MTSRPQPSAPFAAQAIPFDEYLASGKIPEGLLTSEYIGQQFVER
                     LVHYVLSVPAGSYTMAQLSRLLEELDPRAQVFFFKRLKENSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
     gene            6415..6783
                     /locus_tag="PK614_03980"
     CDS             6415..6783
                     /locus_tag="PK614_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81397.1"
                     /translation="MNPTLQRAVTSFYNLVYEAQTFATTMSRIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDANITPTTLQRVLTEVKSDLDHELSVQRREKGRRADGREEALTFV
                     ADYLSSLITATEIESAKTSV"
     gene            6841..7080
                     /locus_tag="PK614_03985"
     CDS             6841..7080
                     /locus_tag="PK614_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81398.1"
                     /translation="MAREWIIFAVCLGLGGHIALAVVLHAPDLWPWSKAWLYGLLSGV
                     ALYVVVQVIRSLWWIFRGRNAEEPETPEDQRIGHP"
     gene            complement(7590..7886)
                     /locus_tag="PK614_03990"
     CDS             complement(7590..7886)
                     /locus_tag="PK614_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRB81399.1"
                     /translation="MRDIVSDAAGKKVNLDKDLLAILCCPETKQAVALADEQLIQKVN
                     GAIERGALKNKAQKPVTEKLDGGLIRSDNKILYPVREDIPVMLIDEGIPLEQVS"
     gene            8030..8656
                     /locus_tag="PK614_03995"
     CDS             8030..8656
                     /locus_tag="PK614_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRB81400.1"
                     /translation="MTPSHRLGRIFLTGLLVLLPAWTTFLILAALFETLDSFLLNLIG
                     RQIQPYAPGLGLLLLIGMVLTVGAIATQVIGQRVVRWTEAVLDRIPLIRSIYMTLKGM
                     TDLLNYRARFGQSTVVAFPFPRDGLWALGFVMGSPPAALQIAPMVELVMVFVPTAIHP
                     FTGYLALIPKTQLHPLNLLPEEALKLEFSAGLYRPLPGWLTSPARNPS"
     gene            8653..9171
                     /locus_tag="PK614_04000"
     CDS             8653..9171
                     /locus_tag="PK614_04000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB81401.1"
                     /translation="MSLADSLTVRPATLEDLDALTTFSAAMALETEQRTLDHARLRLG
                     TRAVLEQPERGRFYVADLRPEPPADRVTVGQLLLTYEWSDWRNAQFWWIQSVYVHPDW
                     RRKGIYRSMHGAIINLARSQPGVCGVRLYVEGDNHIAKCVYERVGLSPSSYQVYESDF
                     VLPTPHSARTKA"
     gene            9207..9587
                     /locus_tag="PK614_04005"
     CDS             9207..9587
                     /locus_tag="PK614_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81402.1"
                     /translation="MRRVSVLLVLMLLTLNACAFSRGTLGDEIKTETVNSIKKGATTR
                     SEVLALLGAPDRLLQLNGRDLFQYYRYDAKVGSLLLIVLNFSRVSVKSDDLFVVINRE
                     GTVEEVIASKRTEGLEFRFWPFGD"
     gene            9592..10014
                     /locus_tag="PK614_04010"
     CDS             9592..10014
                     /locus_tag="PK614_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81403.1"
                     /translation="MTGWNRSVTLLFSAVIALALSGCNVARLTINTPLTPESVAFIAE
                     GRTTLADVVDTLGAPDSITDTETGTIVTYRFLNAKYSRVNFGWLLKPWSPVDPDLVFS
                     RSGLGTDAFELLCNDNWIVTHHAFVRHRPAPPFTPYPF"
     gene            10082..10939
                     /locus_tag="PK614_04015"
     CDS             10082..10939
                     /locus_tag="PK614_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRB81404.1"
                     /translation="MGAGSSDESSSQQAPATSSSYIPADHDIDFLQRDELRPLRLGLE
                     LLKPELIQTEQGIRSTIVVFGSARLLEPSRARQALEQASAALQACPDDRTCQQRVAVA
                     ERRLALSRYYDVAREFGRLVSSTCQIDGQCDYVIVTGGGPGIMEAANRGAADAGAKSM
                     GLNITLPHEQRPNPYITPDLCFQFRYFALRKMHFLLRARALVVFPGGFGTLDELFETL
                     TLLQTGKTRNITIILMGQAFWERLINWPLLIEEGLVGPEDLALFHFAETAQQAWDLIS
                     RSHGGPSAR"
     gene            10936..>11947
                     /locus_tag="PK614_04020"
     CDS             10936..>11947
                     /locus_tag="PK614_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB81405.1"
                     /translation="MKLSFHGAARSVTGSRHLLEMPGNRILLDCGLFQGQRQEADRQN
                     RFLGFDPRSIQAVLLSHAHIDHSGALPVLGKHQFRGNVHMTRATVDLAAVMLEDSARL
                     QENDCAYLNKKENRRGKRCLQPFYESRDARAIIRRFVGARYGDTIKVTPRVTASFHDV
                     GHILGSAAIRLKYSARGNSTTVLFSGDLGRSQMPILRDPTPPPSCDVLIIESTYGDRL
                     HEEAGEALTQKAQQLVAHAKEHKSKIIVPAFALGRTQDLVMRIKRLVAEGRIDPLPIY
                     IDSPLASKVTDVFRKHPECYDEETFRTFTSEGDPFAARYIRYVSSPEESKRLNEMKGP
                     CVI"
BASE COUNT         2778 a         3356 c         3202 g         2611 t
ORIGIN      
        1 aagaggtcta ttagccatta atgatcgtgc aacttcaggc tgccttccgt tcccttctcg
       61 gcccagcctt tgacgggtat agctgtggga atgccgcggc taggagcgat ctgcaaaaaa
      121 tcacactcaa ggaattagtc aaaaacggat ggcctgaaga aacgacgcca cttatacaga
      181 cacgggtcac atacccagaa aatattagta agaataaccc cgattataaa tgccgggctc
      241 ttctacacgc gaaggtaggt gaaaggggat atgagtcgtt ttcgtttgca gtctattaca
      301 actatgacga ctacaaagtg actgattaca aatctgactt tacgaaatat cagcgaatga
      361 aaattgccga tctgttggct ggcaaattag aaccattagc tcaaaaaatg aaggtgtgtt
      421 ctcagcaaga aaaagcggag ggatgtgaag atggcagaga ccgactccat taatcatctg
      481 acccataagg ggcttctcat tatcgtaatg gccctcctag ggatttcatt ctcattgggc
      541 gacggacaga catggagttt ctcccgatcc gcgttcgccg aggaaattgt cccatccaat
      601 gatacctctt tcgaacagac caaaccatgt agcctctggc tgtctggcac cccgagacca
      661 gcaacatcca cattttctaa gccgttgggg gacaccaatg caaacaataa ggggccggct
      721 gacgcattga gggaatactt agcacaagtt caagctcgaa tccgtcaatc ctggtcaatt
      781 attccgatta atctccccaa tagagccttg acgactatca taaatttccg gctagagcgg
      841 gcgggaaggg ttagcaatgt aaccattcaa cagtcttccg gcaatgaata taccgatctc
      901 gcggcacgtc aagcgttaca gagagctgtt cccttgccac cgtttcctcc gtatatagcg
      961 gatcgttatt tagatgccgc ttatactttt ttcgtcgagg agttgaagaa aacatcagac
     1021 cagcctcgta gcggcctcat tcgtggtcat gccctcgctg gcacagaatt aatcgtgacc
     1081 gatcagtccg cttcagaaga gtcctggact agccaaaggt atcaatatgg tgactgtcgc
     1141 aagcatttag aaacgttgac gcaggtaatc gatgaagtag cggatagtca agcaggacta
     1201 agcaggctgc aacacattgc caatgattta cccttttccg aaaacaatac cgagtcattt
     1261 gagaactcag acatagtctc actcgttgag tcactacgct atagaattaa agcgaggtct
     1321 gaagaaatca aagttaagct cgtcaccgat tccgaaatgg agcggttcga caatgtgtgc
     1381 aagcctgaga tactgaaatc cggatttcct gatcgtctcc gcgatgagcc tgttgcctca
     1441 tcgcgtccgg agaccaagac gctcgggaga gccttttgta ctgctatcaa cggaggggga
     1501 aaagttgagt tccatgtaat tgacgagaag acacccacgg ttggtatttt cgtcaccacg
     1561 aagaaacgac gatttgtcgt cgtactgcag aaaaagatgc aaggagattt gagtgaggaa
     1621 tggacagcta ttgagattca aacaccttct gatcggaaac cgattattaa tatgttggga
     1681 tattccttca tggatatgcg attcccggca ttggtacgga atgaatagta gcaaccatat
     1741 ctaggcttga ttgatattat gggagcccgt atcaaccaac ggcgcaagga tttccttatt
     1801 gatagagact aagcggcttg ttatgaacaa ctattcggaa ggtaggctaa ttggcgaata
     1861 ttttaaagca gagcagcagc gtctgttgtg ctacatgcgt gaaaatgaag cccctcaagt
     1921 atgctggcct gatggatgtt atgggtcagg cctgccgtgg ctcacatttg tcgggccaag
     1981 ccctggggga gggaacggaa ggcagcctga agttgcacga tcattaatgg ctaatagacc
     2041 tctttggaat gaggattata ccgatccttg ccaattgtgg agcccgggtt ttcgcacaag
     2101 tataagaatg cttattgaga tcattcttgg tcgatcgagt gagttcggtg cactgaggct
     2161 ctataatttt ctcaactttg attggatgca gaacccaaaa gccagtgagg tgccacgaga
     2221 gcgaatgcgc cgaggtgcag aggttgtgtt ggatcatttg gaatccagtc gaccccgtac
     2281 ggtcgtcacg ctggaaagta gctcccactc tcttcttaag gaaatgctct ctaggaagta
     2341 tcagcttcgg ccagcggagt ttggagatgt atccatactt tcttacaaga ctcatgctca
     2401 tagggcaatg gatgcctttg aagtccttgg agatactggt cttggtggat cttttgtctt
     2461 gcgatgcccg caacatcctg ctcggatctt tcgccaagac tatgcaggca gagtgggacg
     2521 tgcgctgcgg catactttgg tttctcttgc cgaagctcag agaccgattt caatttacga
     2581 acaataatcg aataattggt gaagctgatc aaaattccaa tactcccatc ggtatacttg
     2641 ctctcgtctc gatcggcctt aaagcagtcg cgtcggggcg gctgccgccc tccacatatc
     2701 gaattaatct taaagccaga agttctccct tagaagtgtc tcgtcgtctg ggatcttacg
     2761 ggcctatccg tatcacgcct gcctcttctt gacatgtcat tcaatatgac gtacatattc
     2821 gtcattcatt atgaatgaca tggaggtccc gtgaaaacca caatgatgca gtttcgggtg
     2881 aatgaagaag aaaaagcctt aatcgaaaag tgtgccaaga agggtggcat gaccgtatcc
     2941 gagtacattc gggcctgcat gctgatggag atggtcattg atggtgaagt ggcagctctt
     3001 cgcatcatcg gtcggaccat tggcatgaag gcgatggacg ctatgaagag acgcctggcg
     3061 aggaacccgg taacggaatg aacggtgtaa tgaagcatgg caaagccggt ccgcgccgca
     3121 tgccgatagg ctgcggggcg gagcggaatg gcccccgtct tggtaacacg ggggcgctaa
     3181 ccacagcgct ccgtacagga ggtcgataga ttccatggat tcagccttca ccctctccat
     3241 tgattggctc gcgtttaccg tcctggcgag caggccacaa gagaccatga aggtgcttgg
     3301 gggagagtgg agtaaggcga aaggaggctt cagaggctat cccctgtcct ggatcaaatt
     3361 ggacggactg catggagttg gaaaactcgg caccaatgcg ccacgtcgtc ccaatgaaat
     3421 ccatgtggat ctctccggcg ggctcgtctc cgcactcacg ctcgatcagg ttcgagccct
     3481 gctgaagtgg gtgcatgagc agcaaggcca cgttacccgt gttgattgtg ccctcgatga
     3541 tcgagcagga accgtctccg tggcgacagt tcgagaagcc gtgtcggccg gtcagtgtgt
     3601 cacacgagcg gcacaggtcc ggcatatcgc ctccaacctg acgcatggca cgggagccag
     3661 tactggtgag accctgtatt tcggcagtcc gcagagtcag acgttgctcc gtatctatga
     3721 caagcgactg gagatgcaaa gcaaggaacg tgagaactgg caagactatg gcgtccggtg
     3781 ggaattggag ctcaagaagg atcgggcgga acaatgtgct cgtgcgttgg cgtctttaaa
     3841 tgaaaccgat tggaaggagt ttgtcgtcgg actgctccgg tcctatgtcg atttccgaca
     3901 gattacgaaa gagacggaag atgaagaccg ctaccgcgcc ccgatgctgg agtggtacgc
     3961 cctcctgacg gagggatttc agaagggccg attagcccag gagaagcagg tgcagaccct
     4021 gcagaacgtg aaacgatggg tgagccatgc cctgacgcca ttgctggcgg taatttgtgc
     4081 cacccctgga ggggaagaat ggctacagca agagattgtc aggggcattg ctcggtggaa
     4141 ggaccgacac cgaaacttgc tcaaacaacc acccaatcgg tttcacacgt ctgccggcgg
     4201 ccacgcgggc agcccatgtt agggggactg ggggtgtcgt cagactcccc cggtgatctg
     4261 caccatgctt accacgaaag agctttcggc ttggctcaac atcaagccgt ccacactcta
     4321 cctctgggcc tcacagaaca agattccttg ccgtcgcatt cacggcctca tccgattcga
     4381 tcctgacgcc atccaggcct ggttgaacag ctttggtgtg actcctggaa agccaccctc
     4441 ccttcccatt cgccatgtca gccgtgacct agaccagctc attgaagccg caaaacgtga
     4501 ggtctatact ccccgccacg gggaaaccat tcagtcgagc ctcatccgga aggaggatgc
     4561 agatggggct atttaggagg aataaagtgt ggtggatgac attcgtccat caaggccaac
     4621 agataagacg ctccacaggg acaacagacc ggcgtttagc tgacgcaatt ctgggaaaag
     4681 taagggtcaa gattgtcgag ggccgattct ttgacacgct ggaagccaaa gaccacacct
     4741 tcaacgagat gatggaccgt tatctaatag agcgatccgt cacgaaggca ccggcaagca
     4801 gactgcggga tgagcagtgc ctcgtgcatc ttagaagtgc gtttgggact ttgagcctgg
     4861 cggatgtgac gccgaaactt ctcgccgcct ataaagcaca acggcgcgag agagccaaac
     4921 cagcgacagt caacaaagag ctgggactgg tccggcatag cttcaacgtg gccattcgag
     4981 aatgggaatg gtgtcgggac aaccccatgc agaaagtctg catggagaaa gtgcgcaatg
     5041 aacgagaccg gtggctgtct tcagatgaag agggtcgatt actggctcat tctgcaccat
     5101 ggctccagga cataattgtt tttgccttaa acacgggcat gcggcgaggc gagattctca
     5161 acttgcagtg gcaagccgta gatctcactc ggcaggtctt ggtggttatg cgaagcaaga
     5221 atggagagaa gcgcacggtt ccgctcaacg gtcggttaac ggaattgcta cagagaaagg
     5281 cgccaaaaaa gacgacaggc tcgttcgttt ttgctagttc agctgaaacg ctagttgacg
     5341 ctcgaaacct cacacgagca ttcagactag ccgcgaagaa agcagaattg atcgactttc
     5401 gttttcacga cttgcggcat acgtttgcca cccggctcgc acaagccgga gtcgatttgt
     5461 ataaggtaca acgcctgctg ggacacaaaa cagcggctat gacgcaacgc tatgcgcatc
     5521 actcgcctga gagtctcaga gagggagtgt tgattctgga gcgcgttcac cctcagatat
     5581 cacaaaagta tcacaatgag ggtgcgaccg tcggggggtg ttgtgcaagt gcttgagaaa
     5641 attggagcgg gaaacgggat ttgaacccgc gaccctcgcc ttggcaaggc gatgctctac
     5701 cactgagcta ttcccgcttc caccgacaga agaagcggga ttctaccgac catgcgtaaa
     5761 gactgtcaag ccgggcagca gaaatatcgt cattacagcg actctacgta gctgcctgcg
     5821 cggggccact gtcaaagccg gccatctgca cgaagcgcgg gtggcccctt ttctcgcaaa
     5881 catacagcct ttcgactcag acctgcgtgc ttcttctgtc cacttgacgg acctgcgggg
     5941 cttgagtaga gtgattccaa tgtcggccca ctccatcctc atcagcgagg tacccttagc
     6001 gtgacctcac gtcctcagcc gtccgctcct tttgcagcac aagccattcc cttcgacgag
     6061 tatctcgcat cgggcaagat ccccgagggg cttctcacga gtgaatacat cgggcaacag
     6121 tttgttgagc gattggttca ctacgtactg agcgttcctg ccgggagcta cacgatggca
     6181 caactcagcc gtcttctcga ggagctggat ccacgcgccc aggtcttctt tttcaagcga
     6241 ctgaaagaaa atagtccgga tagtctgaaa gattttgccc cgctgtatta cggattcatg
     6301 aacgagtttc attccctcct gtttacgtaa gtcactgccc agtaaaaaaa taccttgtcg
     6361 gccccccatt ctcaggtata atcacgacaa cctcttaatg agccaaggag cactatgaat
     6421 cccacgttac agcgcgctgt tacgagtttt tataatctgg tgtacgaagc ccagactttc
     6481 gcgaccacca tgtctcggat cgacactgcc gagcaggagc actatgccgg gcggatcgag
     6541 gggctgaact gggtcctgga ccgctgtcag gagcttgagg acatggatgc caacatcact
     6601 cctacgacac tgcaacgtgt cctgacggaa gtgaaatcgg acttggatca tgagctctcc
     6661 gtacagcgac gcgaaaaagg ccgacgggcc gacggacgcg aggaagcctt gaccttcgtg
     6721 gcggactact tgtccagcct gatcacggca actgagatcg aatccgccaa aacctccgtg
     6781 taaccgcctg tcgattgtct ccaggcttat ccttcgtcat ggcctcctcc gagggcgact
     6841 atggcccgtg agtggatcat cttcgccgtt tgtctcggct tgggaggcca cattgccctt
     6901 gccgtggtgt tgcacgcgcc tgacctgtgg ccatggagca aagcctggct ctacgggttg
     6961 ctctcaggcg tcgcgctcta tgtcgtggtt caggtcatcc gttcactctg gtggattttt
     7021 cgcggcagga acgctgagga gccggaaaca cccgaggatc aacgtatcgg ccatccttga
     7081 ttccaatcca ggcaggcatc gtcctgttgc accgcatcga tctccctcag tagaataccg
     7141 ctcatgcagc cacgttcgaa caaaccctcg ccgcccaatt ggcctccaag cgccatgcga
     7201 ccacgccccg caccgtcaca gcctggtcaa cgccagacag atcaatatga ctcgttcaac
     7261 gcctccctgc tgtactgcgg acaatgtcgc caggcaactc ctacgcgcca acggctcctg
     7321 ctcgtcctgc cgacagggaa tctctacgaa tacctctgcg ctcaatgcgg agcctccacc
     7381 ggctcaaaga cggaaagtgc ttcggccgaa ggtggactgc tcgccccgta accattcgtg
     7441 tcgcaatcca gtgccagacg gtcgcttcgg cgcgtacgct cacgcatgac acgccgagtt
     7501 ataagccggg atcgtcaacg cccatggcaa gcgcgaacgc ctgccaaggg agtactcgca
     7561 ttggagaaga gaggaccgag ggagggtaat caggaaacct gttcgagagg gataccttcg
     7621 tcgatcaaca tgacaggaat gtcttctcgt acggggtaga gaatcttgtt gtcggatcgg
     7681 atcaatcctc catcgagctt ctccgtgacc ggcttttggg ccttattttt gagcgccccg
     7741 cgctcgatcg caccgttcac cttttgaatc aactgctcgt cggccagagc cacggcctgc
     7801 tttgtttcgg ggcaacacag tatggctaac agatccttgt cgagattcac tttttttcct
     7861 gcggcatcgc tcacaatatc tctcacaatc ccacccgata ggggtgactg aacggttacc
     7921 ggaaagatag tccaaatgaa tcgacagatc aagaagatac caaatcgccc ccctttctga
     7981 tatcgtcatt gatctattcc cccttcactc accaacatcg ggacgcgacg tgactccttc
     8041 gcatcggctc ggacgcattt tccttaccgg actcctcgtg ctccttccgg cttggacaac
     8101 gtttctcatt ttggccgcgc tgttcgagac actggattcc ttcttgctca atctcatcgg
     8161 ccggcagatc cagccctatg ccccgggact cggcctcctg ctgctcattg gaatggtact
     8221 cacggtcggg gccattgcga cccaggtcat cggacaacga gttgtacgct ggaccgaggc
     8281 cgtcctggac cggattccct tgattcgcag catatatatg acactcaagg gcatgacgga
     8341 tttactgaac taccgggcac gcttcggaca aagcaccgtg gtggccttcc cttttccacg
     8401 cgacggcctt tgggccctgg gttttgtcat gggctctcca ccggcggcac ttcaaatcgc
     8461 accaatggtg gaattggtga tggtctttgt cccgactgcc atccatccct tcacgggata
     8521 cttggccttg attcccaaaa cacaattaca cccgctgaac ctgttgccgg aagaggcatt
     8581 gaagttggag ttttcagcgg gactctatcg ccccttacca ggctggctga cgtcgccggc
     8641 acgtaaccca tcatgagtct agccgatagc ctgaccgtac gtcccgccac actggaagac
     8701 ctcgacgcgc tcacgacatt cagcgccgcc atggccctgg aaactgagca acgtacactg
     8761 gatcacgcac gcttacgcct gggaacccga gcggttctcg aacagcctga acgagggcgg
     8821 ttttacgtgg cagacttgcg gccagagccg ccagccgata gagtaaccgt aggccagctg
     8881 ctcctcacgt acgagtggag cgattggcgg aacgcccaat tctggtggat tcagagtgtg
     8941 tacgtacacc ctgactggcg acgcaaaggc atttatcggt cgatgcatgg cgccatcatc
     9001 aacctcgctc gatcacaacc gggtgtgtgc ggtgtccggc tgtacgtcga aggcgacaat
     9061 cacatcgcca aatgcgtcta tgaacgagtc gggctctcgc catccagcta ccaggtgtac
     9121 gagtctgatt ttgtcctacc caccccccac agcgcccgca caaaggcgta agttagtccg
     9181 atcgacgtcc gcataaggag gatcacatgc gacgagtgag tgtgctgttg gttctgatgc
     9241 tcctgacact gaatgcctgc gcgttcagcc ggggcaccct cggcgacgag atcaagaccg
     9301 aaaccgtcaa ctccatcaag aagggagcga ccaccaggtc ggaagtcctg gctctactcg
     9361 gtgctcctga ccgactcctt caactgaacg gacgtgatct ctttcagtac taccggtacg
     9421 atgccaaggt cggcagtctg ctgttaatcg tgctcaattt ttcgcgcgtg tcggtcaaga
     9481 gcgacgatct gtttgtggtg atcaaccgcg aagggaccgt cgaggaagta atcgcgtcaa
     9541 aacgaacaga gggcctggaa ttccgcttct ggccattcgg agactaaggt catgacaggc
     9601 tggaaccgct ctgtcacgct gctgttctcc gcagtcattg ccctggcttt gtccggctgt
     9661 aacgtggctc gcctcacgat caacaccccc ctgacgccgg aatcggtagc cttcattgcg
     9721 gaaggccgca ccacactggc cgatgtcgtc gacacactcg gagcaccgga ttccattacc
     9781 gatacggaaa caggaaccat cgtcacctac cggttcctca acgccaaata ttcgcgggtc
     9841 aatttcggct ggctactgaa accctggtcg cccgtggatc ccgacctcgt gttctcgcgc
     9901 tcaggcctcg gcacagacgc gttcgaactc ctgtgcaacg acaattggat tgtcacccac
     9961 catgccttcg tgcgtcaccg ccccgcaccg ccgttcacac cctatccgtt ctgacccctc
    10021 tgcacatgcg cctcgcatcg gtgaattgcc gcccccggca catctcgtta taatgccgct
    10081 catgggtgca ggttcttccg acgagtcatc gagccaacag gcccccgcca catcatcctc
    10141 ctatatcccg gctgatcacg acatcgactt tctccagcgc gacgaactgc gccccctgcg
    10201 cctcgggctg gagttgctca agccggaatt gatacagaca gagcagggta tccgctcgac
    10261 gatcgtggtc ttcgggagcg cacggctgct ggagccatcg cgcgcacgcc aagccctgga
    10321 acaagcctcg gccgcattgc aggcctgtcc cgacgatcga acctgtcagc aacgtgtcgc
    10381 cgtagccgaa cgccgcctgg ccctctcgcg atactatgat gtcgcccggg aattcggccg
    10441 gcttgtgtcc tcaacctgcc agatcgacgg gcaatgcgac tacgtcatcg tcacgggtgg
    10501 aggcccgggc attatggagg cggccaatcg tggagccgcg gacgcaggcg ccaagtccat
    10561 ggggctcaac atcacgctgc cgcacgagca gcggcccaat ccctacatca cgccagacct
    10621 ctgttttcaa tttcggtact tcgcgctgcg caagatgcat tttctccttc gagcccgggc
    10681 gctggtcgtg ttccccggtg ggttcggtac actcgacgaa ctctttgaga cactgaccct
    10741 tctccagacc ggcaaaacgc gcaacatcac gatcatcctg atggggcaag ccttttggga
    10801 gcgcctcatc aattggccgc tgttgattga agaaggcctg gtgggccccg aggacctcgc
    10861 gctcttccac tttgcagaaa cggctcagca ggcctgggat ctcatcagcc gaagccatgg
    10921 aggcccgtcc gcccgatgaa actctcattc cacggagccg cgagatccgt tacgggaagc
    10981 cgccacctgc tcgaaatgcc cggtaataga attctcctgg attgcggact ctttcaggga
    11041 cagcgccagg aagcggatcg tcaaaaccgg tttctgggat tcgacccgcg ctcgatccaa
    11101 gccgtgttgt tgtcccatgc ccatatcgac cattccgggg ctcttcccgt gctgggcaaa
    11161 catcagttcc gcgggaatgt gcacatgacc cgcgcgaccg tagatcttgc cgcggtgatg
    11221 ctggaagatt cggcccgcct gcaggagaac gattgcgcct atctgaacaa gaaagaaaac
    11281 cgtcggggca agcgctgcct gcaacccttc tatgagagcc gcgatgcccg cgccatcatc
    11341 cgtcgcttcg taggcgcgcg gtacggggac acgatcaagg tcacccctcg cgtgaccgct
    11401 tccttccacg acgtcggcca cattctggga tccgccgcca ttcgcctcaa atattccgcg
    11461 cgcggcaaca gtacgacagt gctcttctca ggtgacttgg gacggtcgca gatgccgatc
    11521 ctccgcgatc cgactccgcc gccaagctgc gatgtgctga tcattgaatc cacctacggc
    11581 gaccgcctgc acgaagaagc cggggaggcg ctgacccaaa aagcccagca acttgtcgcc
    11641 catgcgaaag aacataagag caagatcatc gtgcccgcgt tcgcgctcgg gcgcacgcaa
    11701 gacctggtca tgcggatcaa acgattggtg gcggaggggc gaatcgaccc gctcccgatc
    11761 tatatcgatt cgcctctggc ctcgaaagtg acggacgtct tccgaaagca ccccgagtgt
    11821 tacgacgaag agacgttccg cacgttcaca tcggaagggg atccctttgc ggcccgatat
    11881 atccgctatg tctcctcgcc agaagaaagc aaacgattga atgagatgaa aggcccctgc
    11941 gtgatca
//
LOCUS       DAORQN010000037        11867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_316_length_11867_cov_10.643075, whole genome shotgun sequence.
ACCESSION   DAORQN010000037 DAORQN010000000
VERSION     DAORQN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_11867_cov_10.643075"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..559)
                     /locus_tag="PK614_04025"
     CDS             complement(128..559)
                     /locus_tag="PK614_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRB81406.1"
                     /translation="MPTADEVGTPFDIIQWSDLLKSASALEMYYKRYGRIAPDDVAAF
                     LILEPTFPRAIRYCLIKAEDSLHAISGSERGSYQNLAEKRLGRLRAELDFVDIEDCVT
                     RGLHEFVDHFQARLNQVGEAISETFFVPRRVHYTESVTEEQ"
     gene            515..1198
                     /locus_tag="PK614_04030"
     CDS             515..1198
                     /locus_tag="PK614_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81407.1"
                     /translation="MDDVERRADFIGGRQEDEIFDIENARGFVRSFEEAISESDQTFD
                     KLFKAVNVFAAQIRRVAEEDKSALYNAGLTFNLHALVGGQLENDLTHKLYLIYPQGNW
                     VEVSEGTPYCIIGESGYGKPLLDRVLRYNSSMDLALKVGFLAFDATRTSSTSVEYPLD
                     VVLYRHDTFDILEHRFQKEDLAEISSWWQSRIADSVEKLPASWVDRLLEALPKGTRSS
                     PQDHGNSAS"
     gene            2002..2598
                     /locus_tag="PK614_04035"
     CDS             2002..2598
                     /locus_tag="PK614_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB81408.1"
                     /translation="MTSSQKLLRVTTLLGFALVCATVLWPSHALPENAPVTLPIETVA
                     NYLHAVIEADRDVYTRHVVERLQTKGVVVASENWEQKNTLPLPAQFLMESGRYIAKKG
                     LGIQYRLISLWPINKRNVAANELEKAGLGTILTQPNRPHTGFMKIGETRYFQAVYADL
                     AATQACLGCHNAHPDSPKRDFKLNDVMGAIVITIPVGQ"
     gene            complement(2717..3619)
                     /locus_tag="PK614_04040"
     CDS             complement(2717..3619)
                     /locus_tag="PK614_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81409.1"
                     /translation="MLRITSFNSCFLGLTILLCLGCTAPTLTSRVIEQDSSWFVRLDS
                     LLEAGSASPRYDHPSVWRDEELFAILSRLLLQERVGLMDNARPPRSVFSLEEINFLMP
                     TVRKSFQEATSREWIVFTLVGQEGTGRETTSGGMFVEEGKLHLVIANHRLALAENSEE
                     LARVRANPLHSVQGSGGALVFDLPRFVIGTKANWSGGHRASASELILDHAAFLSSLQH
                     RGSAAAPVPVSGSHAPVSAPEADAPGPGSFHSGQVDSEQIILQLREEIEVLKQQLADK
                     DAEIDRLKPREIRTTPAPVIRTPP"
     gene            complement(3690..4112)
                     /locus_tag="PK614_04045"
     CDS             complement(3690..4112)
                     /locus_tag="PK614_04045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRB81410.1"
                     /translation="MAGKILLEVVTPEKLLLSQEVDEVIAPGSEGEFGVLPGHCHLLS
                     SLRIGELRYKTQDAWHYMSILWGYAEVTPSKVTVMAEIAEKAEDIDVGRAQQAVEKAE
                     QRLQAGGLPSEVKEAQISLEKARLRKKIADRAKKSGRA"
     gene            complement(4238..5680)
                     /gene="atpD"
                     /locus_tag="PK614_04050"
     CDS             complement(4238..5680)
                     /gene="atpD"
                     /locus_tag="PK614_04050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRB81411.1"
                     /translation="MSTGKVIQVIGPVVDVEFPPGQLPNIYNALKVTQEENKAAGKPA
                     VRITLEVASHLGENRVRGIAMSTTDGLTRGMDVQDTGAPISVPVGRETLGRLINVLGE
                     PVDEKGPIKAKKTYPIHRPAPRLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFGGA
                     GVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDHTKSKA
                     ALVYGQMNEPPGARLRVALTGLAVAEFFRDEENQDVLLFVDNIFRFTQAGSEVSALLG
                     RMPSAVGYQPNLSTEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDA
                     TTVLSRQLAELGIYPAVDPLDSTSRILDPQVIGEEHYKVARGVQSVLQRYKDLQDIIA
                     ILGMDELSEDDKMAVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFKEILEG
                     KYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            complement(5749..6636)
                     /gene="atpG"
                     /locus_tag="PK614_04055"
     CDS             complement(5749..6636)
                     /gene="atpG"
                     /locus_tag="PK614_04055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRB81412.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILAARP
                     YAHKMRGVLSNLSQRVNRTSHPLLQKREGKKIEILVVTSDRGLCGGFNGNIVRKSSEF
                     VRQCEAQGLSVNLSLIGRKGRDYFRRRAWPIRQEWTGIFDKLSFEHAIDIGGDLTENF
                     VKGTFDELYVVYNEFKSAIQQRVIVEKLFPIDAAAEFGAAPSATEQSAVGGSYLYEPD
                     EGELLNVLVPKHFQIQAYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTR
                     QAAITKELMDIVGGAEALK"
     gene            complement(6701..8218)
                     /gene="atpA"
                     /locus_tag="PK614_04060"
     CDS             complement(6701..8218)
                     /gene="atpA"
                     /locus_tag="PK614_04060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRB81413.1"
                     /translation="MQIKAEEISSIIKEKIKGFDQQVDVSQTGSVIQVGDGIAKIYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGEA
                     LIGRVVNAIGQPIDGKGPINSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCIYVAVGQKRSTVARVVKTLEENHAM
                     EYTMVVAATASDPAPMQFLAPFSGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVPV
                     DKVLQFEADLLHYVAQNHQDMRKDIATVGKIDDKVGGKLKEIITTFKQKMGYGAK"
     gene            complement(8221..8760)
                     /gene="atpH"
                     /locus_tag="PK614_04065"
     CDS             complement(8221..8760)
                     /gene="atpH"
                     /locus_tag="PK614_04065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRB81414.1"
                     /translation="MIKTAVARRYAKALFELLDTPSIEPARSALNGLGEAFTQSAALR
                     HAIASPVFPEETKLGALIELATRLGCPPVGQRFLELLVKKNRVSFLPDIAEAFAKLVD
                     QSKGTQQVLVSSANALPAAEQDRITTRLRDLLQRDVDVTFHTEPEHVAGLHIRLGSTV
                     VDSTVRGRLSAMQRVLTKE"
     gene            complement(9010..10092)
                     /gene="mnmA"
                     /locus_tag="PK614_04070"
     CDS             complement(9010..10092)
                     /gene="mnmA"
                     /locus_tag="PK614_04070"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRB81415.1"
                     /translation="MTRQTVLLGMSGGVDSSVAASLLVRQGYDVHGVTLQVWEHEDDQ
                     VAVSKRWEERGCCKIGIAKYVAQRLRIPYEVVDRREVFQQGVIDDFVAGYASGTTPNP
                     CVRCNERVKLRSLYALAHERGMDYVATGHYARVQQIDRQWSLHRALDARKDQSYFLYR
                     INPAWLPHLLFPVGHMQKCDVWQEAESLGLPVEELKESQEICFVSHGDYRTFIEQEMP
                     EAKKPGPFVGVDGEVLGQHEGIAFYTPGQRRGLGIAVGQRLYVQKVVPESGQVVLCAE
                     DQLVQSECQVADLSLFDHALGRQPVEAAVKIRYATPPCPATLLPSESGTLQVQFHQPQ
                     RALSPGQSAVFYDGDRVLGGGIIQRS"
     gene            complement(10125..10580)
                     /locus_tag="PK614_04075"
     CDS             complement(10125..10580)
                     /locus_tag="PK614_04075"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB81416.1"
                     /translation="MWGETKKQPVAEDDKFTFLGKGTSFKGIVTFDGTVRIDGRVEGE
                     VHTGGAVIVGESAVIKGVIAAGSVSISGRVKGSVTALQKVEIQKPGILIGDIQSPVIM
                     IEEGAHFHGMSNMGAEKWTEDESSEFGAPQDPGVRDLVAHRGKVKTQEP"
     gene            complement(10710..11198)
                     /locus_tag="PK614_04080"
     CDS             complement(10710..11198)
                     /locus_tag="PK614_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB81417.1"
                     /translation="MAWINKDKQDGKRQAGQSEGTEMENLESTAPREGNEEINAFVGK
                     GVSFKGVISYNGTVRIDGNLDGEIHTEGVLLVGEDAVLTAKITAGTVVCKGKITGDIS
                     AREKVKLRAPAVVNAGVKAPLLAMEEGVVFNGTLEMSQSGTREPQGQGSTRGQGVKLV
                     NG"
     gene            complement(11367..>11867)
                     /locus_tag="PK614_04085"
     CDS             complement(11367..>11867)
                     /locus_tag="PK614_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRB81418.1"
                     /translation="RRRPDHPHDPMETARAYQRMLNEFGLTQEAIAQKVARDRSSVAN
                     LLRLMSLPSEVQHMIESDQLSTGHAKVILGLMTPAAQVSLATQIVSGQLSVREAERLV
                     QEHAEARKPGKRPVRAPLRSDLEERLQKRLGTRVDVQKGRRGGKIVIHYFSPEELDGV
                     VERLLN"
BASE COUNT         2444 a         3627 c         3206 g         2590 t
ORIGIN      
        1 aacatcgagt ggcggccatg ttgatggatg gagacctttc ccgcggtgat gcattccgcc
       61 cctgtcgtca cccgcgtgtc ggcgatcccg accaacccct cctccacctt cattcccaag
      121 caaaacgtca ttgctcctcc gtgacggact ctgtgtagtg aactcggcga ggcacgaaga
      181 acgtttcaga aatagcctcg cctacttggt tgagtcgtgc ttgaaagtgg tccacaaatt
      241 catgcagacc ccttgtgacg cagtcttcga tgtcgacaaa gtctagttcg gcgcgcaggc
      301 gtcccaatcg tttttcggcc aggttttgat acgatccccg ttcggatccg ctaatcgcgt
      361 gcagggagtc ctccgcttta atgaggcaat agcggattgc ccgcgggaag gtgggttcaa
      421 ggatgagaaa ggcggccacg tcatccggag cgatgcgtcc ataccgtttg taatacatct
      481 ctaaggcgct cgcggatttc aataaatccg accattggat gatgtcgaac ggcgtgccga
      541 cttcatcggc ggtcggcaag aggatgaaat atttgacatc gagaatgcgc gaggttttgt
      601 ccgctcgttc gaggaggcca tcagcgagag cgaccaaacg ttcgacaaac tgttcaaagc
      661 ggtcaacgtg tttgccgcac agatccgccg tgtggccgag gaagataaaa gcgccttata
      721 taatgctgga ctgaccttca atctccacgc cctggtaggc gggcaacttg aaaacgacct
      781 cacccacaaa ctttatctga tctaccctca aggcaattgg gtcgaagtca gtgaaggcac
      841 cccctactgt atcatcggcg aatccggcta cggaaagcct ctcctcgatc gcgtgctgag
      901 gtataactcc agcatggacc tcgcgctgaa ggtcggcttc ctcgccttcg atgctacacg
      961 tacgagctcc accagcgtcg aatatccgct cgatgtcgtg ctgtatcggc acgatacgtt
     1021 cgacattctc gaacaccgct ttcaaaaaga ggacctagct gagatttctt cttggtggca
     1081 atcgcgcatt gccgattcag tggaaaagct tcccgcctcg tgggtagatc gtttgctgga
     1141 ggctctgccg aaaggcactc gctcttcccc tcaggaccac ggcaattcag catcgtaagt
     1201 ccggctcgcg attctccgct agcccggcca acgaccgaca aactgtgtcc cgatgacacg
     1261 tcacgatcaa tcgcgccgtc gaccaagctc gatgcactgg ccgcgccaca catctcttcc
     1321 cagtccaaag gtcgatatca ccgggaagga tcgccactgc gtttcaatcg aagaatttcc
     1381 gcttccggct cagccatctg cagtttcaat cgcttgatct tttcttggag tcgaccgagc
     1441 acacggtcgt gatcgagtcc ccactgacca gcgaatggcg tacggtttga cgcgacctcg
     1501 ccgctcgccc acgttgccct gatctcgtgc gcaccgaagg tcgtacttca catcatggcc
     1561 cgagccattg cgatatatgc gacaagatga cgggtaaaga aatggacgta tttcccctag
     1621 tggccaccga tggtgtcgtg ccctcccata agatgattag acagcgtata gatcgaagcg
     1681 taatatgatg agtgaccgta tgatctggtg ggcacgttgg ttcgtacggg caaggagctg
     1741 gggcgatgcc ttcggtacct tcccacgacc ggtcgttttt tcaggaggcc atcgataggg
     1801 ccggcatccc ggctcatcgc tccgccccgt cctccgcgtc gatacctctc aactcattat
     1861 cctcattcac ccggcgatcg atcccgttcg tcctctccac ttcaccaagc actcaccggc
     1921 cagaccgtat ggtgcaattc atctgctcac gtggtgcgcc ggccagaccg gatcacccgt
     1981 gtgcgcaaca aggaggttgg tatgacatcc tctcaaaaat tgctgcgggt cacgaccttg
     2041 ctgggatttg cattggtctg cgcgactgtg ttgtggccca gtcacgcact gcctgagaat
     2101 gcaccggtca ccctgccgat cgaaacggtg gctaactacc tacacgccgt gattgaagca
     2161 gaccgggatg tgtataccag acatgtcgtc gagcgcttgc aaacaaaggg tgtggtcgtg
     2221 gcatcggaga actgggagca gaagaatacg ctgcccctac cggctcaatt tttgatggag
     2281 tcgggacggt acatcgccaa gaaagggtta ggaattcagt accggctgat cagcttgtgg
     2341 ccgatcaaca agcgcaatgt tgcggcgaac gagcttgaga aggccgggct cggaaccatc
     2401 ttgacgcaac cgaatcgacc ccacaccggc tttatgaaga tcggggagac gcgctatttt
     2461 caagcggtgt atgccgatct cgcagccacc caagcctgcc tcggttgcca caacgcgcat
     2521 cccgacagcc ccaaacgtga tttcaagctg aatgatgtga tgggtgcaat cgtgattacg
     2581 atccccgtcg ggcaataatg ccgcggatgt tcagcactct gtcactcagg cgccacgaca
     2641 ataccggcag gacgcaccac gatcctgccg gtgggaacag gtccggtcct tcacctaaaa
     2701 ccttgtcgag tctgtttcag ggaggagtac ggatcacggg agccggtgtg gtgcggatct
     2761 cgcgtggctt caatcgatca atctctgcgt ccttgtctgc gagttgctgc ttcagtactt
     2821 cgatctcttc ccgaagttga agaatgatct gttctgaatc cacctggccg gagtggaatg
     2881 atcccggccc tggtgcatca gcttctggtg ccgagactgg cgcgtgcgat ccactgacag
     2941 gaacaggagc cgccgctgat cccctgtgct ggagagagga aagaaatgca gcatgatcga
     3001 ggatcaattc gctggcggac gctcgatgcc cacctgacca attggctttt gtcccgatca
     3061 caaatcgagg taagtcaaag accaaggcgc cgccgctgcc ttgaacggaa tggagaggat
     3121 tcgcccgcac acgcgcaagc tcttccgaat tctccgcgag cgcgagacgg tgattggcga
     3181 tcaccagatg gagcttgccc tcctcaacaa acatgccacc ggacgtggtc tcacgacccg
     3241 tcccctcttg gccaaccaac gtaaagacga tccattcacg ggatgtcgct tcttgaaagg
     3301 acttgcgcac agtgggcata aggaagttga tctcttccaa cgagaatacc gaacgcggcg
     3361 gtctcgcgtt atccataagg ccgactcgtt cctgaagcaa caatcggctg aggatggcaa
     3421 agagttcttc atccctccaa acactcggat gatcatatcg tggggatgca ctgccggctt
     3481 cgagaaggga gtcgagccgc acaaaccagg aggagtcctg ttcaatgaca cgactcgtta
     3541 gcgtgggcgc ggtacaaccg agacagagga ggatcgtgag gccgaggaag caagaattga
     3601 atgacgtgat tcgtaacacc ggacgtcccc cgacagcgga tcagggaggt gcaaaatcgg
     3661 cagactgcgt gaagtatcgg gtagaggggt taggcgcgcc cggatttttt ggcacggtct
     3721 gcgatctttt ttcggagacg ggccttttcg aggctgatct gagcttcctt cacttcggaa
     3781 gggagcccac cggcctgcag tctctgttcg gccttttcga cggcctgttg cgcacgaccg
     3841 acatcgatgt cttcggcctt ctcggcgatc tccgccatca cagtcacttt ggatggtgtg
     3901 acctcggcgt agccccacag gatcgacatg tagtgccacg cgtcctgagt cttgtaccgc
     3961 agctctccga tgcgcaggct ggagagaaga tggcagtgtc caggcaggac accgaattct
     4021 ccctcgctac ccggagcgat gacttcatcg acctcctggc tcaggagcaa cttctccggc
     4081 gtcacgactt ctaatagaat cttcccggcc atcttctctt acaactccct tgtcccactt
     4141 gagcctgaaa cgatgcgtcc tctcctgcgg gcgtccaacg gggtcttgcg agaccgcgcg
     4201 ttgcgacgag cagcgaggac gcgcggtttc cgcgctccta aaccttgact cccatctttt
     4261 ccgccttcgc gacggcttcc tcgatcggac ccaccatgta gaaggcctgc tccggcaggt
     4321 ggtcgtattt cccttcgagg atttccttga agctgcggac ggtatccttg agcttcacgt
     4381 acttgcccgg tgcgccagtg aacgcttcgg ccacatggaa cggctgggaa aggaagcgct
     4441 gaattttgcg tgcacgggcc acggccatct tgtcgtcttc cgacaattcg tccataccga
     4501 gaatggcaat gatgtcctgc aaatctttat accgctgcaa cacggactga acgccgcggg
     4561 ccaccttgta atgctcttct ccgatgactt gcggatcgag aatacgtgac gtggaatcga
     4621 gcggatcgac cgccgggtaa atacccaact cagccaactg ccgggacaac acggtcgtcg
     4681 catccaagtg cgcaaaggcc gtcgccggag ccgggtcggt caagtcgtcg gccggcacgt
     4741 aaatcgcctg caccgaagtg atggagccct tcttggtgga cgtaatccgc tcctgtagcg
     4801 cacccatttc ggtggacagg ttcggctgat aaccgaccgc cgatggcata cggccaagca
     4861 acgcggacac ttccgatccc gcctgcgtga accggaagat attgtcgacg aacaacaaca
     4921 cgtcctgatt ttcttcgtcg cggaaaaatt cggcaacagc gagtccagtc agcgcgaccc
     4981 gtagacgggc tccgggtggc tcgttcatct gaccgtagac cagtgccgcc ttggacttcg
     5041 tatggtcgtc gggatcgatg accttggact cctgcatttc gtgccaaagg tcgttaccct
     5101 cacgggtacg ttcaccgacg ccagcgaaca ccgagaatcc accgtggtgg agcgcgatgt
     5161 tattgatgag ctccatgatg atgacggtct taccgactcc ggctccgccg aagagcccga
     5221 ccttaccacc cttgctgtac ggctccaaca gatcgaccac cttgataccg gtctcgagca
     5281 cttcggtctt cgtgtcctga tcttccagtc gcggcgcagg ccggtgaatg gggtaggttt
     5341 tcttcgcctt gatcggaccc ttttcatcga ccggctcacc caacacgttg atcagccgtc
     5401 ccagggtctc acgaccgacg ggaacagaaa tcggggcgcc cgtatcctgc acatccatgc
     5461 cgcgagtcag accgtccgtt gtcgacatcg cgatgccgcg aacgcggttc tcaccgagat
     5521 gcgatgcgac ttcaagcgtg atccgcacgg ccggcttccc ggctgccttg ttctcttcct
     5581 gcgtcacctt gagcgcattg tagatgttcg gcaattgacc ggggggaaat tccacgtcga
     5641 ccacggggcc gatgacttga atgacttttc ctgtgctcat aaggctcctc ttgtcgtcaa
     5701 tgatgcaccc ggcacattac cgtgatgcag cattcctcat tccacatttt atttcagggc
     5761 ttctgcgccg cccacgatgt ccatgagttc tttggtgata gccgcctggc gagtcttgtt
     5821 gtaatacaag gtgacttttt tgatgagttg ccctgcattg cgagtcgcac cgtccatggc
     5881 tgccatgcgt gccccgtgct cggccgccgc cgactccaga aggatgcgat aggcttgaat
     5941 ctgaaaatgc ttcgggacca agacattcag aagctcgccc tcatccggct catacaagta
     6001 actaccgccg actgcggact gctcggtcgc actcggcgcc gctccgaatt cggcggccgc
     6061 atcgatgggg aacaatttct cgacgatcac ccgttgctgg atcgcagact tgaactcatt
     6121 gtagacgacg tagagttcgt cgaacgtgcc cttcacaaaa ttttccgtca ggtcgccgcc
     6181 gatgtcgatg gcatgttcga agctcagctt gtcgaagatt ccggtccact cctgtcggat
     6241 cggccaggca cgacggcgga aatagtcacg ccccttacgg ccgatcaggc tgagattgac
     6301 cgagagcccc tgcgcttcgc actgtcggac gaactctgag ctcttccgca cgatgttgcc
     6361 gttaaatccg ccgcacaagc cgcggtcgct cgtaacaacg agaatttcga ttttcttccc
     6421 ctcgcgtttt tgaagcagcg ggtgggaggt acggttgacg cgctgactga gattgctgag
     6481 caccccgcgc attttatggg cgtaaggcct cgcagccaaa atgcgatcct gcgaacgctt
     6541 gagcttcgcg gccgcgacca ttttcatggc cttggtaatc ttttgcgtat tcttgaaggc
     6601 ggcgattttc cgccggagag attgtaagct cggcattctt gtacgctctc agcagtcagc
     6661 gctcagacct cagctccatg ctgaaggcga agactgattg ctattttgct ccgtagccca
     6721 ttttctgctt aaaggtcgtg atgatttctt tcagcttgcc gccgaccttg tcatcgatct
     6781 tcccgaccgt ggcgatatct ttccgcatgt cctggtgatt ctgcgccacg taatggagca
     6841 ggtccgcctc gaattgcagc accttgtcta cggggacatc gtccaggaat ccgttgacgc
     6901 ccgcgtaaat cgacaagact tgatcggcaa ccggcatcgg cttgtattgc ccttgcttga
     6961 gcaattccac catacgtacg ccacgggcga gctgcatctg ggtcgccttg tcgagttcgc
     7021 tcccaaactg ggagaaggcg gccatttcac gatattgcgc gagatccaac cggagcgtac
     7081 ctgcgacctg cttcatagtt ttgatctgcg cggatccgcc gactcgggac accgacaatc
     7141 cgacgttgat cgcgggacga ataccggaat agaacaagtc gctgcccagg taaatctgcc
     7201 catcggtaat cgaaatgacg ttggtcggaa tgtaggccga cacgtcgccg gcttgcgtct
     7261 cgatgatcgg aagggccgtc aggctgcctc cgcccaactt atcgctcaac ttcgccgcgc
     7321 gctccagcaa gcgggagtgc agatagaaca cgtcgcccgg ataggcttca cggcccggcg
     7381 gacggcgcag cagcaaggac agctgacgat aggccaccgc gtgcttggac aaatcgtcgt
     7441 acacaatcaa cgcatgcttg ccgttgtcgc ggaaatattc accgatcgcg gcgccggaga
     7501 agggggccag gaactgcatc ggcgccggat cgctggccgt cgccgccacg accatggtgt
     7561 attccatggc gtgattttct tcgagggtct tgactacccg cgcaaccgtc gaacgcttct
     7621 gaccgacggc gacgtagata cagaagacac cgaggccctt ctggttgatg atggtatcga
     7681 cggcaatagc tgtttttccg gtctgccggt ctccgatgat caactcacgc tgaccacgac
     7741 cgatggggat catggcgtcg atggccttga tgcccgtttg cagcggctca cgcaccgact
     7801 gtctcgtgtt cacaccgggt gccacgactt cgatacggga agaatgttgg gaattgatcg
     7861 ggcctttgcc gtcgatcggc tgaccgatcg cgttcaccac ccgtccgatt aacgcttcac
     7921 caaccgggat ttccgcaata cgcccagtgc gcttgaccgg atcgccttcc ttgatcccga
     7981 catcgtcacc catcaacacg gcgccgacat tgtcttcttc gaggttcaac gcgatgccgt
     8041 acaggccccc cgggaactcg agcatttctc cggccatcgc gccgtctaat ccgtagattt
     8101 tcgcgatgcc gtctccgacc tgaatgacgg aaccggtctg gctgacatcg acctgttgat
     8161 cgaagccctt gatcttctct ttaatgatgg agctgatctc ttctgccttg atctgcatgg
     8221 ctactccttg gtcaacacac gctgcatggc gctcagtcgg ccccggaccg tgctgtctac
     8281 gacggtgctg cccaggcgga tgtggaggcc ggcgacatgt tcaggttctg tatggaaggt
     8341 gacatcgacg tcgcgctgta aaagatcgcg caatcgggtg gtgatccgat cctgctcggc
     8401 cgcgggcaag gcattcgcgg atgacacgag cacttgttgc gtccccttgg attgatccac
     8461 gagcttggcg aaggcctcgg cgatgtccgg caggaaactg acccgattct ttttgaccaa
     8521 gagttcgaga aatctctggc cgaccggcgg gcatccgagt cgagtggcca attcaatcag
     8581 cgcacccaat ttggtttctt ccgggaacac aggagaagcg atggcatggc gtaacgcagc
     8641 ggactgcgtg aaggcctctc ccagcccgtt caacgccgag cgggcgggct cgatgcttgg
     8701 ggtatcaaga agttcgaaca gagcttttgc gtatcgacgt gcgacggctg tcttaatcac
     8761 aatggactat ccgctacggt taaccgtgtt ttgatcaaac gggaagcttg cgtgcggtgg
     8821 gcacgacaaa aagcgcgcca agttagcatt caaaagcggg tactgtcaag aagtgatgcg
     8881 cggggagacc gcagatgaga catcgcaacg agcaggagcc ccgcgccagt gccgcttgcg
     8941 cacggcctcc actgagcccc cgcttcgcaa aattgattta ctggagaaaa aattggagag
     9001 tggcactggc tatgaccgct gaatgatgcc gccaccgagt acgcggtcgc catcataaaa
     9061 cacggcggac tgacctgggc tcaaggcgcg ctgcggctga tgaaattgaa cctgaagcgt
     9121 gccgctttca gacggtagaa gggtcgccgg gcagggaggc gtggcgtaac gaatcttcac
     9181 ggccgcctca accggctgcc gaccgagggc gtgatcaaac agactgagat cggcgacctg
     9241 acactctgac tgcacaagct gatcttccgc acataacacc acctggccgg attcaggcac
     9301 cactttctgc acgtacaacc gctgccccac ggcaataccc aggccgcggc gttgccccgg
     9361 cgtatagaac gcgatgccct catgctgccc gaggacctcg ccatccaccc ccacaaaggg
     9421 tccgggcttc ttagcctccg gcatttcctg ctcgatgaac gttcgatagt cgccgtggct
     9481 cacgaaacaa atctcttggc tttccttgag ttcctccacg ggcaatccga gcgattcggc
     9541 ctcttgccac acatcgcact tctgcatatg gcccaccgga aacagcaggt gcggcaacca
     9601 ggcaggattg atccgataga gaaagtaact ttggtctttg cgcgcatcaa gcgcccgatg
     9661 cagcgaccac tgcctatcga tctgctgcac gcgggcatag tgccccgtcg ccacatagtc
     9721 catgcctcgc tcatgtgcca aggcatacag ggagcgcaat ttcacccgct cattgcaccg
     9781 cacgcaggga ttgggcgtgg tgccggaggc atacccggcg acgaaatcat cgatcacgcc
     9841 ctgctgaaag acttcgcgac gatcgacaac ctcatatgga atacgtagac gttgcgcgac
     9901 atacttcgcg atcccgatct tgcagcatcc ccgctcttcc cagcgcttgg agacggcgac
     9961 ctgatcatcc tcatgctccc atacctggag cgtgacgccg tggacatcgt acccctgccg
    10021 cacgaggagc gaggcagcga cggagctatc cactccgccg ctcataccta gcagtacggt
    10081 ctgacgtgtc ataaatggta ccggaggaac aatgcgccga gcacctacgg ctcttgcgtt
    10141 ttgactttgc cgcgatgggc tacgaggtcg cgcacgccgg gatcctgcgg agcaccgaat
    10201 tcagaggact cgtcctccgt ccacttttcg gcgcccatat tgctcatgcc gtgaaaatgc
    10261 gcgccttctt cgatcatgat caccgggctc tggatatccc cgatgagaat ccccggcttt
    10321 tgaatctcga ccttctgcag agccgtcacc gaccccttca cgcgtccgct gatcgagacg
    10381 gatccggcag caatcacgcc cttgatgacg gcgctttctc cgacgatcac cgccccgccc
    10441 gtatgcactt ccccctcgac acggccatcg attcggacgg tgccatcaaa cgtaacaatg
    10501 cccttaaagc tggtcccctt gccgagaaag gtaaatttat cgtcttccgc gaccggctgc
    10561 tttttggtct caccccacat cggcgtcctc cttcacacac cccacacatc ctagagaccg
    10621 gggcgcaggc tgaatggaac cttgctatgc ctacctcttg cttacaaggc aagccagacc
    10681 ctgtccaaca aattcgtcgt tgagccgagt tatccgttga ccaacttgac gccctgtccg
    10741 cgcgtggatc cctgcccctg cggctcgcgc gttcccgact ggctcatctc caacgtgccg
    10801 ttgaagacca ccccttcttc catggccaac agcggcgcct tcacgccggc attcaccacg
    10861 gcaggcgcgc gcaacttcac cttctcccgg gcgctgatgt cgccggtgat cttccctttg
    10921 cacaccacgg taccggccgt gatcttcgcc gtcagaaccg catcctcacc gaccagcaac
    10981 actccttcgg tatgaatctc tccatccaga ttgccatcga tgcggacggt gccgttatag
    11041 gaaatgacac ccttgaagct gacacctttt ccgacaaagg cattgatctc ttcgtttccc
    11101 tcgcgcggtg ccgtagattc caaattttcc atttcagttc cctcgctttg tcctgcctga
    11161 cgcttcccgt cctgcttgtc cttattaatc cacgccattt gttgcacctc ccacctgtta
    11221 caacgtcgtc catgatcgga ccggcaggct ttcaattacc tgccggacgg tcaccactaa
    11281 gagtgtcccc atttaatatc cggtgaaaat cgccgatatt ttactctcgc agcgagcaga
    11341 aaatctcgcc taaaacacat ctgccatcaa ttgagcagcc gctcgacgac gccgtccaat
    11401 tcttccggcg aaaaataatg gatgacgatc ttgccgccgc gccgcccctt ctgaacatcc
    11461 actcgggtcc ccagacgttt ctgcaatcgc tcttcaagat ccgatcgtaa tggcgcacga
    11521 accggacgct tccccggctt tctcgcttcc gcatgttcct gcaccaatcg ctccgcttcg
    11581 cggacggaca gttgcccgct gacgatctgt gtcgccaaac tgacctgcgc ggccggtgtc
    11641 atcagcccga gaatgacctt agcatggcct gtggagagct gatcggattc gatcatgtgt
    11701 tgcacctcgg agggcagcga catcaatcgg agcaggttcg ccacggacga ccggtcgcgc
    11761 gccactttct gagcgatcgc ctcctgggta agtccgaatt cattcagcat gcgttggtac
    11821 gcccgcgcgg tctccatggg atcatgagga tgatcaggtc gccgtct
//
LOCUS       DAORQN010000038        11172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_333_length_11172_cov_6.421427, whole genome shotgun sequence.
ACCESSION   DAORQN010000038 DAORQN010000000
VERSION     DAORQN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_11172_cov_6.421427"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4121)
                     /locus_tag="PK614_04090"
     CDS             complement(<1..4121)
                     /locus_tag="PK614_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRB81419.1"
                     /translation="MGRCERMLRAVGVVLALAFTLVASLPLHAQTVLFGPIQYTRTAG
                     PPDQFTATFALPTGTTAPYTIHIVNGNADGTKRVSSGTIKLNGIQIAGPNDFGQNVAV
                     IDRAVTLQTNNTLEVRLTSAPGSVLTISVLDTGAGSQPTAMTPNPLNLAAGTTGTLTV
                     TLAPASTAAGSLMVSSANPAVATVPASVSFTAGQTTVPITVTAVAAGSTTVTATLNGG
                     SAAGQVTVSPALPTITGVMPTSGQPGTSVTVTGTNYVNVQTVTFNGVAATFTVTDPTT
                     LVAVVPPTATTGPLAVQTFAGTAISATLFTVVPAPTLANLQPATLSIAQGNTGSLTVT
                     LSAVQTTDTTVVLASSTPTVATVPSSITVPATQLSASIVVTAVALGQADITASLNGTS
                     ATSAVTVILGSPSITGINPASGPAGTVVTIAGSSFNPTAANNQIAFNGQAAIVSSVNT
                     AGTALVTTVPQGTTSGPVTVTTLVSPPATGPIFTVTVPDFTVTALPTSLLLPVTGQRT
                     VAVTVVGIHNFSGLVSLSTSDVPAGVSAVFASTTLAAGQSGMLNVTTTGATTAGTYPV
                     TVQATGLVNGSPIDKTVTVNLEVLSGGLTSFAGQVQDEDNQPVKGARVTMGAVHAITD
                     AGGNFLLQNPPVGVDQLFLIDGGPASTPGRNLPIIPYKGTIVAGQANVLSFIPKLHVQ
                     KTTGLVDIADSSVQRVVTDPELPGYQMTIPAGATITGWDGQPNTQVSIRRVPLDQNPL
                     PPIPGDRVATDIYMDYFGKAGGGTASEPIPITLPNDLNLPPGTSSELWFYDEAPDGSR
                     PNQWAKLGTATVSGDGSQLVPDVDPATGKAYGQPRFCCGGIFAAMLRAAFDLINNQVN
                     GQPQTPNGAKGGEPVDLGTGAFNLMKTDMVLPGRLPITITRHYRSNGPTTGPFGPGTS
                     HGYQVVVRAEANVRTLFMPDGSRMQFRQQPDGTFRNSSEPTVRGAVYSEVNGRILRFK
                     DGTRWTFGAGSQGFFFLTALADRNGNQLTLTRSGATLNVTAVTAQDGRRLTLAYDAGN
                     RITQITDPIRRVVQYQYDGSGRLTSLVDPEGGITRYAYDAAGRLVTLTDARALTFLTN
                     EYSPGSSRVLRQTQIDGGIWKFRYQLSGVRVTGPGCPAPGSQDVWVVIEGAPICPREE
                     SVETVAQGYQFVGGVVTAATVIDPLDHTTTYRFNGAGHSVSQTDALGQTTISTRDGAS
                     NQIVSSIDPLGRVTAFEYDLAGNVSKITDPNNQATRFEYHPTWNRVTKITDALNQVTE
                     FTYDPTNGNLLAIKDPLNHVTTITYNGFGQPLSVQGPIATEPPTTFAYDAAGNLLTTT
                     DPLGNQAQRTYDVVSRLLSLTDPRGLVTQFRYDGLNRVTEIADARQGLTRFTY"
     assembly_gap    4521..4557
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4718..5617)
                     /locus_tag="PK614_04095"
     CDS             complement(4718..5617)
                     /locus_tag="PK614_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81420.1"
                     /translation="MTRRRRDFLGSFFFLILFILLLTLGLAIPSFSADLSDREEDGLL
                     GDVQSVETRESLLVQTDRYDPVGRLIERIQGGGETSQGLWPLRFVYTYDQAGRRTAEV
                     VQDARGAVVKETRSVYDDRGNRSAELAAWGDGTFENVSLYEYDEAHRPIRGLHFNGVQ
                     VINRNLYAFDSAGRLVRERFERNYHYNAAGTQVVMTDRFDTGYEVAIVYDEQGYVREK
                     VVSDLRSRPQGRSEFRYDEQGNQNEERIFNAKGQATDRKVYRYEYDAVGNWIKETLQW
                     WAVADGRETLKQSSVRERSISYY"
     gene            complement(5679..6458)
                     /locus_tag="PK614_04100"
     CDS             complement(5679..6458)
                     /locus_tag="PK614_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRB81421.1"
                     /translation="MLLEGKKGLIIGVANKHSIAWAIAQAAAREGAQMLFSYQNERLR
                     ENVEELVQTLPGASACVCDVGDDSQIDAMMKQASEKLGRLDFLVHSLAFAPREELTGE
                     FVNTTRQGFATALDVSAYSLVAVTRAAMPLMTEGGSVVTLTYLGSERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPKNIRVNAVSAGPIKTLAARGVSGISKMVDHHRAFAPLRRAT
                     EQGEVGDTALFLVSPLGRGITGEVIYVDGGYHILGSLASVD"
     gene            complement(6643..7491)
                     /locus_tag="PK614_04105"
     CDS             complement(6643..7491)
                     /locus_tag="PK614_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81422.1"
                     /translation="MAEWLRLGSGTFLLLLLFGWIMAGTGGAAEKVQGILTVHDALTL
                     PNHPVRIEAQLGGKAPGGSSPWGGVGLHLSIDGKPVAMAKTNESGHASFDYLAKMRGT
                     YAVTVSVDDAASVAADNSDATLCVWERRRPIILVELAALIQPVTSASTGTAAESSLQP
                     LSDAADELSRLTQYYYNVVYVTGGEQPPSEIAAIGNVRRWLESHKFPPGFVAPPSEGL
                     GATLETFKQGGWATMKTGIVRTRSSAEMLLQHRFEVVVVPELPKGEMPRKAKAAKEWK
                     DVRKKL"
     gene            complement(7532..7774)
                     /locus_tag="PK614_04110"
     CDS             complement(7532..7774)
                     /locus_tag="PK614_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81423.1"
                     /translation="MDPLEKTSHACRAAALSLVPGLGHLYIGEKRGGPLLLAGVLLIV
                     LWMAVFSFVLVPLIGLVLFCMGDTYLTVSRDRGLLT"
     gene            complement(8131..8370)
                     /locus_tag="PK614_04115"
     CDS             complement(8131..8370)
                     /locus_tag="PK614_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81424.1"
                     /translation="MAIQSPLSFSDSLTLARRTAFYERHKPIAVVMILLVFLLPFFGV
                     FVRGLIGAAAGVMISVLCYYLTPYLVLKLGGWADR"
     gene            complement(8896..9414)
                     /locus_tag="PK614_04120"
     CDS             complement(8896..9414)
                     /locus_tag="PK614_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81425.1"
                     /translation="MKMKRLVGMAVMVIGLVMSDAIEAADLAGAWKGLLSSADGSQAE
                     VQIDFSPQGFPLYSYTNNKGLTRQVELSHVGQTVEYVPPGGGVQRMVVDRVEKGHGRL
                     SVGIVGSFERASQGYLDQRQEAALFEYALVPGGLKMRVTTSWTSHFGDKDLMVGGDPD
                     AVVAEGVLQKLR"
     gene            complement(9443..10354)
                     /locus_tag="PK614_04125"
     CDS             complement(9443..10354)
                     /locus_tag="PK614_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB81426.1"
                     /translation="MATTTSSSSSKPKGLSLHIGLNAVSPAAYGGWSGDLAACEFDAK
                     DMAAIAASRGMKSTVLLTKKGTRAKTLAAMRKAAKALRKGDFFFLTYSGHGGQVPDVT
                     GEEADKQDETWCLYDGQLIDDELYFELSRFAAGVRILVFSDSCHSGTVTRNMPPQFDP
                     NTSIGRSKMMPISVGLRTYREHQKFYDTLQQNVAKAAGKASVADPDSVLAQLAVSPRL
                     TAIAKEFKPSVLLISGCQDNQTSMDGEQNGAFTEQLLKVWNHGNYSGNYAKFHAAIKA
                     GLPASQTPNLFTLGAAARFVRQRPFTV"
BASE COUNT         2259 a         3379 c         3301 g         2196 t
ORIGIN      
        1 tcgtaggtaa agcgcgtgag cccctgccgg gcatcggcga tctcggtgac gcggttcagg
       61 ccgtcgtagc gaaattgagt gacgaggcct cgggggtcgg tgagtgagag gagccgcgag
      121 acgacatcgt aggtccgctg ggcctgattg cccaggggat cggtggtcgt cagcagattc
      181 ccggccgcat cgtaggcaaa ggtagtcggt ggctcggtgg caatcggccc ttgcaccgag
      241 agcggctgcc cgaagccgtt gtacgtgatc gtggtgacat gattcagtgg atctttgatg
      301 gccagcagat tgccgttggt cggatcatag gtgaactccg tgacctggtt caacgcatcc
      361 gtgatcttcg tcacgcgatt ccacgtcgga tgatattcga agcgcgtggc ctggttgttg
      421 ggatccgtga tcttgctgac gtttcccgca aggtcatatt cgaacgccgt gacccgaccg
      481 agcggatcaa tgctgctcac aatctgattg ctggctccgt ctcgggtaga gatggtcgtt
      541 tgtcccaatg cgtcggtctg actcacactg tgcccggccc cgttgaatcg gtaagtcgtc
      601 gtgtggtcca gcggatcgat aaccgtagct gcggtcacca cgcccccgac gaattggtag
      661 ccctgggcaa ccgtctcaac actctcttcc cttggacaga tgggcgcccc ttcgatcacc
      721 acccagacat cctgactgcc aggcgcggga cagcctggcc ccgtgacgcg cacgccgctg
      781 agctgatacc gaaatttcca aatcccacca tctatctgcg tctgtcgcag aacacggctg
      841 ctgccgggac tatactcgtt cgtcagaaat gtcagggctc gcgcatcggt cagggtcacc
      901 agacgccccg ccgcatcata ggcgtaccgg gtgatccccc cttcgggatc aacaaggctc
      961 gttaatcgac cggatccatc atactggtat tgcacgaccc gtctgatggg gtccgtgatc
     1021 tgcgtgatgc ggttcccagc atcataggcc aacgtgagcc ggcggccatc ctgcgcggtg
     1081 acggccgtca catttaacgt ggctccactt cgtgtcaacg tcagctgatt gccgttgcga
     1141 tcggccaggg cggtgagaaa gaagaagccc tgcgagccgg cgccgaatgt ccatcttgtc
     1201 ccatctttga accgcaatat ccggccgttc acttcggaat agaccgcgcc cctcacggtc
     1261 ggctctgaac tattgcgaaa tgttccgtca ggctgctggc ggaattgcat gcggctaccg
     1321 tcgggcatga agagcgttcg gacattcgct tccgcgcgca ccacgacctg gtatccgtga
     1381 ctcgtgccgg gaccgaaggg gccggtggtt gggccattgc tccgatagtg acgggtgatc
     1441 gtgattggca gacgccccgg caagaccatg tcggtcttca tcaggttgaa cgcgccggtg
     1501 ccgagatcga ctggctctcc gcctttcgct ccattcggcg tttgcggctg gccgttgacc
     1561 tgattgttga tgagatcgaa ggcggctcgc agcatagcag cgaagatgcc accacaacaa
     1621 aaccggggtt gaccgtaggc ttttcctgtg gccggatcaa catcgggcac gagttggcta
     1681 ccgtcccccg aaactgtggc tgttcccagc ttggcccact gattaggccg actcccatca
     1741 ggggcctcgt cataaaacca gagttccgaa gatgtcccgg gaggcagatt caaatcattc
     1801 ggcaacgtga tgggaatcgg ttccgaagcg gtgccgccgc ctgcctttcc gaaatagtcc
     1861 atgtagatat ctgtggccac gcggtcgccc ggaatcggcg gtaggggatt ctggtccagc
     1921 gggacgcgac ggatggacac ttgcgtatta ggttgtccat cccatccggt gatggtcgcg
     1981 ccggcaggga tggtcatctg atagccaggc aactccggat cggtcacgac acgctgtacg
     2041 ctgctgtcgg caatatcgac caggcccgtg gtcttctgaa catgcagctt cggaataaaa
     2101 ctcagcacat tggcctgccc agcaacgatg gtgcccttgt agggaataat cggcaagttg
     2161 cgccctggcg tcgaagctgg cccaccatcg ataaggaata actggtccac cccgaccggg
     2221 ggattctgca gcaggaagtt gccgccggcg tccgtgatag catgaaccgc ccccatcgtc
     2281 actcgtgcgc ctttgaccgg ctggttgtct tcatcctgaa cttggcctgc aaacgacgtg
     2341 aggcctccgc tcaacacctc cagattcacc gtgacggtct tgtcgatagg actaccgttc
     2401 accaggccgg ttgcttgcac agtcacggga taggtccctg ccgtagtcgc cccggtggtc
     2461 gtcacgttga gcatgcctga ctggcctgcg gcgagtgtcg tcgaggcaaa gactgccgag
     2521 accccggccg ggacgtccga cgtggatagg gaaacgagcc ccgaaaagtt gtggataccc
     2581 accaccgtga cggcaacggt tctttggcca gtcaccggca ggagtaacga tgtaggcaag
     2641 gcggtcaccg taaagtccgg cactgtgacg gtgaaaatcg gcccggtggc cggaggtgac
     2701 accagtgtgg tgaccgtcac tggtccgctc gttgtcccct gcggcacggt cgtgacgagc
     2761 gccgtgccgg ccgtattcac tgaactcaca atggcagctt gaccattgaa ggcaatctgg
     2821 ttgttcgcgg ccgttggatt gaaactgctc ccggcaatcg tcacaacggt cccagccggt
     2881 ccactggcgg gattgatccc tgtgatcgag ggcgacccga gtatcacggt gacggcgctg
     2941 gtcgcgctgg tgccgttcag cgaggccgtg atgtcggcct ggcccagtgc gaccgcggtg
     3001 acgacaatcg aggcactgag ctgagtagct ggaaccgtga tgctgctcgg cacagtggca
     3061 accgttggag tgctgctcgc cagcacgacg gtcgtatccg tcgtttggac agcactgagc
     3121 gtgacggtca aggacccggt attaccttga gcgatcgaga gcgtggccgg ctggagattc
     3181 gcaagggtcg gcgcgggaac cacggtaaac agcgttgcac tgatcgctgt gccggcgaag
     3241 gtctgcaccg cgagtggacc ggtggtggcc gtgggtggca cgaccgcgac gagtgtcgtc
     3301 ggatcggtca ccgtaaacgt ggccgcgaca ccgttgaacg tgaccgtctg cacgttgacg
     3361 tagttggtgc cggtaactgt gaccgacgtg ccaggctggc cgctggtcgg catcacgccg
     3421 gtgatagtgg gcaaggcggg gctcacggtg acttggccag ccgcactccc accattgagc
     3481 gtagcggtca cggttgtcga accggcagca acggccgtca cggtgatggg tacagtggtt
     3541 tgtccggccg taaacgacac ggaggccggc acggtggcca ccgcgggatt cgcgctactc
     3601 accatcaacg atccggcggc agtggacgct ggcgctaagg tgacggtcaa cgtgccggtc
     3661 gtaccggctg caaggttcag cggattcggc gtcatggccg tgggctgaga tccggcgccg
     3721 gtatccaaga cactgatcgt gagaacgctt ccgggtgcgc tggtgaggcg gacttcgagt
     3781 gtattgttcg tttgcaatgt gacggcccgg tcgatcaccg cgacgttttg accgaaatcg
     3841 ttagggccgg cgatctggat gccgttcagc ttgatggtcc cagaggacac ccgcttggtc
     3901 ccatctgcgt tgccattgac gatgtggatg gtatagggcg cggtcgtgcc ggtaggaagc
     3961 gcgaacgtgg cggtgaattg atcaggcggc ccggcggtgc gggtatattg tatggggccg
     4021 aagaggacgg tctgggcgtg tagtggaagg ctcgctacca gcgtaaacgc cagggcgagg
     4081 acaaccccta cagcccgaag catccgctca catcgtccca tcacacctcc tccggttgac
     4141 ggactgccta tcggctacgc actggctatg agctagtacg tactagctcg taacacaatg
     4201 cagcagctat acaggactct cggccgtttg ccaatacggc tgaatggctc tatacatgac
     4261 tccgagcaat ggccctagcc acttcacaat aagtggaact cgttcaggct ctcacgaaag
     4321 cagccacact ccatatcctg tgcataatca ccccaccatc tcgccagatt gtgtctcacg
     4381 gctggcacga tacctctctt ttccgattaa ttacatactg tccgtatacc tttccttcca
     4441 cccgccgtct tacttcatga caggttgatt ggagcgtgcc accggatgag agagccaaag
     4501 cggcgccctt caaaaacaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     4561 gcaggattga tcgccagcct agttgtagcc gtcaccgcac cggtaacgat gccatgaata
     4621 caaagcggac gcaggacgct tcgatccaca cgccaacctc gtcaaggatc gtttccgacg
     4681 cggcacggcc atacgacctc tcaaaccagc aggcgcctta atagtacgaa atgctccgct
     4741 cccgaacaga ggattgcttg agcgtctcgc ggccatcagc cacggcccac cactggagcg
     4801 tttccttgat ccaattcccc acggcgtcgt attcgtagcg gtagactttg cggtccgtgg
     4861 cctgtccctt ggcattgaag atgcgttctt cgttttgatt gccctgttcg tcgtagcgaa
     4921 actccgaacg gccctgtggt cggctccgga gatcggacac gactttctcg cgaacatacc
     4981 cctgctcatc atagacgatg gcgacttcat acccggtatc gaatcgatcg gtcatgacga
     5041 cctgagtccc ggctgcattg tagtgatagt ttcgttcgaa ccgctcacgc accagccgtc
     5101 cggcactgtc gaaggcatag agatttcgat tgatcacttg cacgccattg aagtgaagcc
     5161 ctctgatcgg ccgatgtgct tcgtcgtatt catacaagga gacgttctca aacgtgccgt
     5221 cgccccaggc tgccagttcg gcggaccgat tcccgcggtc atcgtagacc gaacgcgtct
     5281 ctttcacgac ggcgccccgc gcatcctgca caacctcggc agtccgtctt cctgcctggt
     5341 cataggtata gacaaatcga agcggccata acccctgaga tgtctccccg ccgccctgaa
     5401 tgcgctcgat gaggcgaccg accggatcgt accgatcggt ttgcaccagc aggctctctc
     5461 tggtttcgac ggactggacg tcacccagca acccgtcttc ctcccgatcg gagagatcgg
     5521 cagagaagga gggtatggca aggccaagcg tgagaagaag aatgaagaga atcaggaaga
     5581 agaacgagcc gagaaaatcc cgcctgcgcc gcgtcatgac atcggctcga tcaggcaggg
     5641 cgtacgacac gaactgtgcg ggcagccccc caggcctctt agtcgacaga ggccaatgac
     5701 cccaggatat gataaccccc atcgacgtag atcacctctc cggtgatacc gcgccccaaa
     5761 ggactcacga ggaacaaggc cgtatcgccg acctcgccct gctccgtcgc ccgccgtagc
     5821 ggagcgaatg cgcgatggtg atcgaccatc ttactgatac cggacacgcc acgcgccgcc
     5881 agcgtcttga tcggcccggc ggagacggca ttgacccgga tattcttcgg ccccagatcg
     5941 tgagccagat accggaccgt cgcctcaagc gccgccttgg ccacacccat gacattgtaa
     6001 tgcggcacca cccgctcgct gccgaggtag gtcagcgtca cgaccgatcc gccttccgtc
     6061 atcagcggca tcgccgcccg cgtgacggcc acgagggagt aggcgctgac atcaagggcc
     6121 gtcgcaaatc cctgacgggt ggtattcacg aattctccgg tgagctcttc ccgcggagca
     6181 aaggccagcg aatgtaccag aaagtctaac cgccccagct tctcagaggc ctgtttcatc
     6241 atcgcatcga tctgactgtc gtctccgaca tcgcacacgc aagccgacgc gccgggcagg
     6301 gtctgcacca actcctcgac gttttcgcgc aaccgttcgt tctgataact gaacagcatc
     6361 tgcgcgcctt cacgagcggc agcctgcgcg atcgcccagg cgatgctgtg cttgttggcc
     6421 acaccgatga tcagaccctt tttcccttct aacaacatac cggctcctgc tccttcaatg
     6481 aaatgacact gtgtcgggcg ctgaacatac catgattcgc cccgtgagtg aatcgccctc
     6541 tcgccatgaa cgctcgcagt accttctcgc ccatgatcac aggccgcggg caaacccaaa
     6601 gaccctgcga cgttcgcgcg cgcacacgtt ctccgacacc cctcacaact tctttctgac
     6661 gtccttccac tctttcgctg ccttggcctt tcgtggcatc tcgcctttgg gcaattcggg
     6721 gaccaccacc acctcgaatc gatgctgcag gagcatttct gcagacgacc gcgtgcggac
     6781 gatgccggtt ttcatagtcg cccagccgcc ctgcttgaag gtctccagcg tggcgcccag
     6841 gccttccgac ggcggcgcca caaagccggg cggaaacttg tgagactcca accatcgacg
     6901 cacatttccg atcgcggcaa tctccgacgg cggctgttcg ccacccgtca catagaccac
     6961 gttgtagtaa tactgggtca accgcgacaa ttcgtcggct gcatcggaca ggggctgcag
     7021 ggatgattca gccgccgttc ccgtcgaggc ggaagttacc ggctggatga gggctgccag
     7081 ttcgacgagg atgatcggac gccggcgctc ccacacacag agcgtcgcgt cactgttgtc
     7141 cgccgcaacg gacgccgcgt catcgaccga taccgtgaca gcgtacgtgc cccgcatctt
     7201 cgccaggtaa tcgaacgagg cgtgcccgct ctcgttagtc ttcgccatcg ccaccggctt
     7261 gccgtctata gacaggtgca agccaacgcc accccatggg gaacttcccc ctggagcctt
     7321 cccgccgagt tgggcctcga tgcggaccgg gtgattcggc aacgtcaacg catcatgtac
     7381 ggtcaggata ccctgcacct tctcggcagc cccgccggtg cccgccatga tccagccgaa
     7441 gagaagcagc aggaggaacg tgcctgaccc gagacgaagc cattctgcca tgagacggtc
     7501 cacgctccct ttcagaggac aaccggcttg gttaggtgag gagtccgcga tcgcggctga
     7561 cggtgagata ggtatctccc atacaaaaca gcaccagccc gatcaacggc acgaggacaa
     7621 acgagaacac cgccatccag aggacaatca gcagcacccc ggccaggagc agaggaccgc
     7681 cgcgcttttc cccgatgtag agatgtccga gccccggcac gagggagagc gcggcggcac
     7741 ggcaggcatg agaggttttt tccagcggat ccatgtcatt caaccaagca gtcgacccgt
     7801 atggtggatc gccgccctcg caggcggcgg aaaaactcga ttgttgcgca atgcgctacg
     7861 atcactcatg cgctgtgtcg gaattacaag aacccgcagg atgcgcaaaa aggctgtcca
     7921 gcaaggccgc agcaagcgaa gcggcgaatc gttcattcgt acccacccac cccgagcctg
     7981 ccaagacagg ctcttgtccc gtggcggtac ggtgagcctc tgaggttcac gacgcgcgga
     8041 ataacgcgcg tcacgtttgt gaacgccgcc gagccggtga ggcggcagtg tcttgcgaga
     8101 acgccgctgg cggactttat cgacagcctg ctagcggtcc gcccatccgc cgagtttgag
     8161 caccaggtag ggcgtgaggt aataacacaa gactgaaatc atcacgcccg ccgcggcgcc
     8221 gatcaacccg cgcacaaaga cgccgaagaa cgggaggagg aagacgagga ggatcatcac
     8281 caccgcgatg ggtttatgcc gctcatagaa ggcggtgcgt cgcgccaagg tgagcgagtc
     8341 ggagaaagac aagggggatt gaattgccat gggatcgcgc ctattccatt ttgcccggat
     8401 tgcgtgtatg aacgtggggc actgacatcg acagggtact agcaggctgc ggaaaaactc
     8461 gcttgttgcg caatacgaga cgatccactc ctgcgccgtg tcggtatggc agtaacccgc
     8521 aggatgcgca aaaagtccgt ccagcaaggc cgcagcaagc gaagaggcga atcgtactcg
     8581 cgccgtacgg tgagcccctg agtgccgcga gaacgccgct ggcggacttt ttccgcatcc
     8641 tgctactcat tcatttacca cgggaggtta ggtatggcga agggcatgca gcagaaaaag
     8701 gcgaccaaga agaagccggc taagacgctg atggagaaac gcgctgaaaa aagcgcgaag
     8761 aagggccgct gattcggttt cgttcgtgca tcaccgccgc ccgatgattg cgacgctcac
     8821 ctgtgtgacc gggaggccgc cgaaccgcac gcctttccgg tcacgcaggc ggtcgactgt
     8881 gctccagtgc ccgcctcaac gcaacttctg caggacaccc tctgcgacga cggcgtccgg
     8941 atcgcctccc accatcagat ccttgtcgcc gaagtgggat gtccaactgg tggtgacccg
     9001 catcttcagg ccgccgggga cgagggcata ttcgaacagc gccgcttcct gtcgctgatc
     9061 cagatagccc tgactcgccc gctcgaagga gccgacgata ccgaccgaca atcgcccgtg
     9121 ccccttctcg acacgatcca cgaccatccg ttgcacgccg cctcccggcg gaacgtattc
     9181 caccgtttga ccgacgtgcg acaactccac ctgccgcgtc agccccttgt tgttggtgta
     9241 cgaatagagg ggaaagccct gcggactgaa atcgatttgc acttcagcct gactgccgtc
     9301 ggcgctgctg agtagacctt tccacgctcc ggccaggtct gccgcttcaa tggcgtcgga
     9361 catgaccagc ccgatgacca tcactgccat gccgacgagc cgcttcatct tcatgaccct
     9421 accctcttgt cggtcgtggt cgtcacaccg tgaaggggcg ctggcgcaca aaacgcgcgg
     9481 ccgctccgag ggtaaagaga ttcggtgtct ggctggccgg caatcccgcc ttgatggcgg
     9541 cgtggaactt ggcgtagttc ccgctgtagt tcccatggtt ccagaccttc agcaattgtt
     9601 cggtgaacgc cccgttctgc tccccatcca tggaggtttg gttatcctgg cagcctgaga
     9661 tgaggagcac cgacggcttg aattcttttg cgatggccgt caagcgcggg ctcacggcca
     9721 gttgggcgag cacgctatcc gggtctgcta cagaggcctt gccggccgct ttggcgacgt
     9781 tctgctgcaa ggtgtcgtag aacttctggt gctcgcgata ggtgcggaga ccgacggaga
     9841 tcggcatcat tttcgagcgc ccgatcgatg tgttgggatc gaactgcggg ggcatgttgc
     9901 gggtgaccgt accgctatgg cagctgtcgg agaacaccag aattcgcacg cccgccgcga
     9961 agcggctcaa ttcgaagtac aactcatcgt cgatcagctg cccgtcgtac aggcaccagg
    10021 tttcgtcctg cttgtccgct tcttcacccg tgacgtcggg gacttgtccg ccgtggcccg
    10081 aataggtcag aaaaaagaaa tcgcctttcc gcagtgcctt ggcggccttg cgcatggctg
    10141 ccagggtctt ggcgcgtgtt ccctttttgg tcagcaggac ggtggatttc atgccgcgcg
    10201 atgcggcgat cgccgccatg tccttggcat cgaactcaca ggctgccagg tcgccgctcc
    10261 agccaccgta tgcggcggga ctcacggcgt tcaggccgat gtgcagggat agtcccttcg
    10321 gtttactgct ggatgacgac gtggtcgttg ccatacgtac ctccttggtc ggcgggacac
    10381 ggtcaggatg aacaagacga tgcaaaaata tggcggtacg gtagtcctta aggacaaatc
    10441 tgtcaacgga gaaggagggg agaatgctgg tatttcaagc gtgtcggcgt ttggaacgtc
    10501 gtgtcgtgag aaaaaacgag atcgcccgaa actgtgaccc cagatgtcac accacggtgg
    10561 tagggtcggg cgaatgcgca gagcaaatgg cttatggccg atagtaagag cggataggcc
    10621 aggccacgtt tctcgtgaag catcgttcgg gtatcccgga aatgtttaat tgagcatgtg
    10681 tcttcaagac gagagacgct tcatgaggca ctcttcacga gatgaggggc cctaggtatt
    10741 tatctaggat ggagtgacga actttgcgcg ggacggctac atcaattctt gctacagtgc
    10801 gctgccctct acagccggtc acgggaggat acgtgataca tcgacgcaac ctctgccagg
    10861 cattagcagc cctggcgctt gccggactat ccagcccgtt tcagaaactc gccgccgcga
    10921 gatcgatagc aatacgaact tccggtaagt cgggtggaag aattcaggat gacgaacaaa
    10981 agctccgtat ctgcattcta agacgccaat ctcatggcgt cttagagaca ttcttagggc
    11041 agccgggcgg tgcgcagcca gacgtatatc ggaaaataga gctgaaaaaa ctgcgataga
    11101 tcgcgcatga attcaacacg gcctccttct gaacaatccg gaaaacgtta cctcgctcac
    11161 gtgcgtcaga at
//
LOCUS       DAORQN010000039        10945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_341_length_10945_cov_19.633150, whole genome shotgun sequence.
ACCESSION   DAORQN010000039 DAORQN010000000
VERSION     DAORQN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_10945_cov_19.633150"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PK614_04130"
     CDS             <1..385
                     /locus_tag="PK614_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB81427.1"
                     /translation="GEPVTEVIHMTKGRRIFLMQCGFHAYMESWGFAVDNPYFALTGD
                     DGTFSLTDVPPGDYTLMVWHPGVGTMLQKKVTVTEKGLSNVDFDFKAPQGRRSVHEIE
                     QNPHYGPESLGKVVDIRPTLQRQVP"
     gene            415..1341
                     /locus_tag="PK614_04135"
     CDS             415..1341
                     /locus_tag="PK614_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRB81428.1"
                     /translation="MRLTRSSLLCCLALVVWLCQVVPSVSAYDIVDVQHGGSLEGRVT
                     LSGAVPDPKAFNLITFPDPTYCGRISNGKGWRLLRDFIVNAESGLKNAVVLLEGVESG
                     KPFDLSVPLIEARDCMFGPWMTIVRNGHAVEVVNMDPVMHDIQGYETSPEAGARVLFN
                     TPLILNQQHQRGNMHAIHNHAPGKSLVGPVYLNKGRRTFYMQCGFHAYMESWAMAVNN
                     PYYAVTDDQGAFRIENVPPGTYQMVVWHPQAGPGVTRTITVGPDGATVEQVALPAPRG
                     TRTAYRVMDNPRFGLESLGHPVEIEPLVEHQH"
     gene            1373..1978
                     /locus_tag="PK614_04140"
     CDS             1373..1978
                     /locus_tag="PK614_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81429.1"
                     /translation="MGAGFSGVLCLSGFGYASATDGPSAIGPAEFSASLVKRVEGRRF
                     EAQVFAKGDRIRLEYKYAVKTELGYSSIEIIRLDKHESWFLLAQRRQILPVPVKPEEI
                     LPIQPSLPGEKSRTLVGDATTIGRASKLYEVRVDYNGRNERFYEWVDVETGVVLKLVS
                     QDRDWSIEYLRIRLSPQPDYYFEEPTGYRRWAPASATQERG"
     gene            2008..3405
                     /locus_tag="PK614_04145"
     CDS             2008..3405
                     /locus_tag="PK614_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81430.1"
                     /translation="MALHNESRSFLTMSGWMAVAMATGVLLCLWPSAASARSLKEAQA
                     AFDKKQYQEALDLVEQVTKEQGPQPEARRLKIHSLILLAKPKDALVEYEHLERDLKQD
                     DRALLKEVALGFVYVLLKDMREQMRGAAYTALKDVDSPEMIPALEDGLSDGSGLVRAL
                     AAEALGKLEAGRRSPRLRNALEDQAGLVKATVLKVLGKSGDRSVIPLLEKALKDEQPA
                     VRLAAAGALYHMGQTAMWDTILKAASAPNPEERATALRMVGELKDARGLSVLLEAITN
                     TQPSVRGAAASALGDLGKVQGIPALEHALEDKIPAVKTSAAISLGELGVKDSLVALRK
                     ALADPNPVVKAAVVSALLRVEEPFESVGEELYELAQNHDPGTRSAAGKAAGRAHGANM
                     KAAIEFLSGMLKDPIPRPRIAAARALGQIGGAEILPMLKLALHDEDDAVRATVGGSIA
                     RILNHAKPSPNRAKS"
     gene            3618..4415
                     /locus_tag="PK614_04150"
     CDS             3618..4415
                     /locus_tag="PK614_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB81431.1"
                     /translation="MKKGAMKVVFGVAAAAFLAAPLASFAGGTIAGKVTYAGKAEQKE
                     FSFSKFPNPKFCPKNPNKSLMDGDKRFLKTIEVAKDGGLKGAVVSVVDIEDQAFQDGF
                     KGTDVTAEFCEFLPFSGVVVNNKNFRTENKDADPDDPKSTLGVLHNPHSFVVKGSSSA
                     TGFNIGLAKKGDKLEKPVTFRGGAEKLGFYRLQCDQHEFMQSFFLPVWNPYHAVVKDD
                     GSFELPGVPAGKHKVVVWHPFVGKGKLNEFEVEVAEGGTANLKAEIK"
     gene            4685..5674
                     /locus_tag="PK614_04155"
     CDS             4685..5674
                     /locus_tag="PK614_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB81432.1"
                     /translation="MPRELSLAEVFQLGYYWETKILLTAVKLDVFSVLDGRGRTASEA
                     AEKLGADVRALELLLNALVAIRLLSKTGDLYANTPVATTHLVKHGPQYIGHLLLLHDA
                     EWGNWGKLEEAVKTGRSPVTRHVFETDPALGANVLSVLHRIGQQSGPDLAKRLALGQV
                     RTMLDLGGGAGTNAIAFCQVYPQLSATVFDLQTTLPLTERTVKDAGLEGRISLKAGDF
                     NRDSLGGPYDVVLMSDILHYQDLATNAALVKKIYSHLNPGGRLIIKDRFLDASGTSPA
                     WTAAFAVHILVNTEQGACYRTAEAMQWMHDGGYVSVEEIERTAVVQGVRPSVG"
     gene            5683..6399
                     /locus_tag="PK614_04160"
     CDS             5683..6399
                     /locus_tag="PK614_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRB81433.1"
                     /translation="MFDFLRQPGFFGTHATMGADLSQLMATLFTGLFIIGWLQAKQHR
                     GHHHHWLMLGGMVTMVGFFTAYYLFRQLGVLAFEGKEGFGGSQALYDYVFIPVLTIHI
                     ILVIIGLVMAVYMIVLGFRSQQFLSGARVLSAARLLTSWKKVSLIFAALLAVVMLLFG
                     TRVMSAGFSMRKLEVYIGFLTLVAIVFAVEMGIQRIWPDGGQRHRALGRFTMIVYCIL
                     FLTGTFTYAMLYILYPGKIG"
     gene            6462..6935
                     /locus_tag="PK614_04165"
     CDS             6462..6935
                     /locus_tag="PK614_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HRB81434.1"
                     /translation="MHTEGVEERISREGDPTWFVRSECRPCQWGVGVDVPVGQVDGLV
                     DRLMWTDDALHRLDRVPPYAVPVLRELVEGFARTRRQRVITYDLIDQAKTGDMVSWDL
                     DAEQRLANVPAPVRAMARVELERTAVDRGECSVTVALMEEVKARYFGMAAQRDDA"
     gene            7012..8097
                     /locus_tag="PK614_04170"
     CDS             7012..8097
                     /locus_tag="PK614_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81435.1"
                     /translation="MLHRLALRVLPSLTLAVTEDSVRTRKRLVMFVPGLVAFAVYRAA
                     KHGMSLAEPVTLLLLSGLVSLVTAICAYRVGRGVPFAQLVSADGARRIGWILAWIGFV
                     YGVQLSLLVLALLWLVGYDYAKHPDGPAMMAMIIPSTAVARDAFEIGYVRWLEASGRP
                     FVTFPDGAGLRALLARVTPALAGWMAAGVAGCVSLSLLLAALVSGDMAVLAQGAVVTM
                     AAGTVGLLAFFAGQGNGAAWTAGLSATRWGELMKFWWWPGLAFASTYYLVLVGGCLYV
                     LKQPMSSPGLFAGVAGLVGGVMALYCYYLGHRRDIENRQPGGVPSALLRCPFVMGILG
                     KSREAIAGTALSGSAAVFDKNSQRASS"
     gene            8094..9962
                     /locus_tag="PK614_04175"
     CDS             8094..9962
                     /locus_tag="PK614_04175"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB81436.1"
                     /translation="MIKRLPLPSVILLLLSGLLVAASVLLLSPPSLVAADPSADVYFH
                     TPGVPDGPSAPTANENHYPQVGSLDSRLLMWFIIQQHTYFGGFVLALPIFCVLLEFLG
                     LVARNPAMAVRYDGLAQDLLKVALLALSVTAAVGSVMLTMFITLYPSFMQYMGGTFKV
                     MMPLYALVFVGTTFLTIAYYYSWDRMAAPGSKWVHLSIGVLAVVFGTSLLLLANAWSA
                     FMMAPSGVDGQGRYLGNPWHLLRSALWNPLNLHRFLADIMSGGAVVLAYACYRFFSGK
                     SQEERAYYDWVGYVFLFVTVCALLPMPFAGYWLMRSVYAYSQSMGVTMMGGLLTWLFV
                     VQALLIGALFLGVNYYLWQSMGRIRGGERYQPYYQYLLLALTGCLFIWLTPHTILMTG
                     TEVKAMGGAQHPVIGNYGVMSSKNGAVNVMICITALSYIFYRRANRTMTISWAKAGNV
                     ILGALFGFGAAHIIGLSVYGFYLPASLRVGLSGPQAVTTLLVVTLGLLLNRRMLRGAT
                     IHGPVQWGQISVRGMVGLFGLAAAFTWVMGLMGYIRSSGRLAWHVNELMPDTSPWAFT
                     PSLGFAAKMVTINMALFWGAVFFLFWVCQRGQQPVMHEDVSAEKEAGFLPSPSHEG"
     gene            9986..10648
                     /locus_tag="PK614_04180"
     CDS             9986..10648
                     /locus_tag="PK614_04180"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HRB81437.1"
                     /translation="MAMKMFVAAIGLLGVVGLCSVMGVGAPAHAGEAGVLVLEDFQSA
                     DQDGFPIDWQHENQRSQAKGRDAYKIQSQDGKKFLAAKDAGQRVKKRKIDWDPKVYPV
                     LTWRWRLHKAPSGSEPIAALYASLDTDLMFIPVFTKYIWSAGKPEGTFVEGGMFSGSE
                     LVVQSGVLPVGEWVEERVNVYEDFKRIHKHEPAEKAWGISLFAGPGVEIDFGTVTVSS
                     GK"
     gene            10683..>10945
                     /locus_tag="PK614_04185"
     CDS             10683..>10945
                     /locus_tag="PK614_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81438.1"
                     /translation="MAGMNPPSTRVLLEVAFGAVVMGTVGALIGAILGGSSIPLASGL
                     GLALGAVVGFLGGRRFLVSILVGTVLGGLLAWFIAGIEKVSFGA"
BASE COUNT         2028 a         2698 c         3641 g         2578 t
ORIGIN      
        1 gggcgagccg gttacggaag tgattcatat gactaagggg cgaaggattt ttctgatgca
       61 gtgcgggttc catgcctaca tggagagctg gggattcgcc gtcgataacc cctacttcgc
      121 gctgacgggt gatgatggca ccttcagttt gacggatgtc ccgcctggcg actatacgct
      181 gatggtctgg catcccgggg tcgggacgat gttgcaaaag aaagtgactg tgacggaaaa
      241 gggactatcg aacgtcgatt ttgacttcaa ggctccgcaa ggacggcgaa gcgtgcatga
      301 aatcgagcag aatccgcatt atggtcctga gtcacttgga aaggtcgtag atattcgtcc
      361 gacattgcag cgccaggttc cctaaggtct gttcgaagag gaaagaggga gatcatgaga
      421 ttgactcgtt ccagcctgct ctgttgtctt gcgctcgtcg tgtggttgtg tcaggtcgtg
      481 ccctccgtct ctgcctatga cattgttgac gttcagcatg gcgggtcact tgagggacga
      541 gtgaccctgt cgggcgccgt tccggacccg aaagcgttca atctcattac gttcccagac
      601 ccgacctatt gtgggcggat ttcgaacggg aaggggtggc gtctcctgcg cgacttcatc
      661 gtgaacgccg agagcggttt gaaaaatgcc gtagtgctgc tcgaaggggt cgagtcgggg
      721 aagccatttg atctctcggt gcccctgatc gaggcgcggg attgcatgtt cggcccctgg
      781 atgacgatcg tgcgcaatgg ccatgcggtg gaagtggtga atatggatcc ggtgatgcac
      841 gacattcagg gctatgagac ctcccccgag gccggggcac gagtgttgtt caatacgccc
      901 ttgattctaa atcaacagca tcaacgggga aatatgcatg cgattcataa ccatgcaccg
      961 ggaaagtctc tggtaggacc ggtctatctg aacaagggac ggcgaacgtt ttacatgcag
     1021 tgcggctttc acgcctacat ggaaagctgg gcgatggccg tgaacaaccc ctattacgcg
     1081 gtgacggatg accaaggcgc gttcaggatc gagaatgtcc cgccggggac gtaccagatg
     1141 gtggtctggc atccgcaggc agggcctggg gtgacgcgga cgattacggt cggtccggac
     1201 ggggctacgg tggaacaagt ggcgcttcct gctcccaggg gaactcggac tgcctacagg
     1261 gtcatggaca atcctcgttt cgggctggag tcccttggcc atcccgtgga gattgagccg
     1321 ttggtggagc atcagcacta agcgcaggtg ggggtgcctc ccacggtggt gcgtgggagc
     1381 cggattctca ggggttctct gtttgtccgg tttcgggtat gcctctgcga cggacggtcc
     1441 atctgccatc ggtccggcag agttttctgc ctcgctggtg aagcgggttg aagggcgtcg
     1501 tttcgaggct caggtttttg caaaaggcga tcgtatccga ctggagtaca agtacgcggt
     1561 caagaccgag cttggctatt ccagtataga aatcatccga ttggataagc acgagtcgtg
     1621 gtttttgctg gctcagcgcc ggcaaattct tccggttccc gtcaagcccg aagaaatcct
     1681 accgattcag cccagccttc cgggggaaaa gagccggacg ttggtgggcg atgctacgac
     1741 catcggccgt gcgtccaagc tctacgaggt gcgagtggac tataacggcc ggaatgaacg
     1801 gttttacgaa tgggtggatg ttgagaccgg cgtggtcttg aaattggtga gccaggatcg
     1861 ggactggtcc atcgagtatt tgcggatccg tctctcccct cagccggact actattttga
     1921 ggaaccgacg ggatatcgac gctgggctcc ggcatccgcg acacaagaaa gaggataggc
     1981 ctcgtggtag gaccaggaag ggatttcatg gcattgcata atgagtcgcg ttcgtttctg
     2041 acaatgagtg gatggatggc ggtggcgatg gcgaccgggg tgctcttgtg cctctggccg
     2101 tcggccgcgt ccgcgcgttc gttgaaggag gcgcaggccg cgttcgacaa gaagcagtat
     2161 caggaagccc tggatctcgt cgaacaagtg accaaagagc aggggcctca accggaggcc
     2221 aggcggctca agattcattc attgattctt ctagcaaagc ccaaggacgc attagtcgaa
     2281 tacgagcatc tcgagcggga cttgaagcag gacgaccggg cgttgctgaa agaagtggcg
     2341 ttgggatttg tgtatgtgct cctgaaagac atgcgagagc agatgcgcgg cgcggcctac
     2401 acggcgctga aagatgtgga ttccccggag atgattcccg cgctggagga tggactaagt
     2461 gacggatccg gactggtgcg cgccttggcg gcagaagctc tggggaagct ggaggcgggg
     2521 cggcgttcgc cgcgcttgcg taatgccttg gaagatcagg ccgggctggt caaggcaact
     2581 gtcctgaagg tgttggggaa gagcggtgac cgctcggtga tcccgctcct tgagaaagct
     2641 ttgaaggacg agcagccggc ggtgcggctt gcggcggcgg gagcgttgta ccatatgggg
     2701 cagacggcga tgtgggacac gatactgaag gccgcctccg ccccgaatcc ggaagagcgc
     2761 gccacagcgc tgcggatggt gggggagcta aaggacgcgc gcgggctgtc ggttctgctg
     2821 gaggcgatca ccaatacgca gccatctgtg cgcggggccg ccgcttcagc cttgggggac
     2881 cttgggaagg tgcaggggat ccccgcgttg gagcatgcgc tggaggataa aattccagcc
     2941 gtgaaaacgt cggcggcgat cagcctgggc gaattaggcg tgaaagattc actggttgcc
     3001 ctgaggaagg cgctggctga tccgaacccg gtggtcaagg cggcggttgt gtcggcgctg
     3061 ttacgggtcg aggaaccgtt tgaatccgtt ggagaggaac tgtacgaatt ggcgcaaaat
     3121 catgatcccg gaacccgctc cgcagccggt aaggcagcag ggagggcgca tggcgccaat
     3181 atgaaagctg cgattgagtt tctttccggc atgctgaaag atcccattcc gcgccctcgg
     3241 atcgccgcag ccagggcact cgggcagatt ggaggggcgg aaatattgcc gatgctgaag
     3301 ttggccttgc atgatgaaga cgatgcggtt cgggctaccg tgggtgggtc gattgcgcgc
     3361 attctcaatc atgcgaagcc atcacctaat cgcgcgaaat cgtaggaggc tttggcggct
     3421 ggatttcaag ttgacagtgt gagtatgatt ccagtataga gaaatgttca gaggcctaag
     3481 cgtagtcgag gtggagccac gtcaagttaa tgcgactgtc ttatacaagg ggtaagggtc
     3541 accagccctt gtgattgagt ggtgacggag taggtgttca gtttcggtta attgttcatt
     3601 actaaggagg cagtgacatg aagaagggtg cgatgaaagt agtattcggc gttgcggccg
     3661 cagcgttttt ggctgcgccc ctcgcctcgt ttgccggagg cacgattgca gggaaagtga
     3721 cttatgccgg gaaagccgag cagaaggagt tctccttctc caagttcccg aacccgaagt
     3781 tttgtccgaa gaacccgaac aagagcctca tggatggcga caagcgtttc ctcaagacca
     3841 ttgaagttgc caaggatggt ggtctgaagg gtgcggtcgt ttccgtggtc gatatcgaag
     3901 atcaggcgtt ccaggatgga ttcaagggca cggacgtgac ggcggaattc tgcgaattct
     3961 tgcccttcag cggcgtggtt gtgaacaata agaacttccg tacagagaac aaggatgccg
     4021 accccgatga tcccaagtcg accttgggtg tgttgcacaa cccgcacagc tttgtggtga
     4081 agggctccag ctctgccacc ggtttcaaca tcggtttggc gaagaagggt gacaagttgg
     4141 agaagccggt gaccttccgt ggcggcgcgg aaaagctggg cttttatcgt ttgcagtgcg
     4201 accagcacga gttcatgcag tccttcttcc tccctgtctg gaatccgtac catgcagtgg
     4261 tgaaggatga cggatcgttc gaactccctg gcgtgccggc cggtaagcac aaagtggtgg
     4321 tgtggcatcc ctttgtcggc aagggcaagc tgaacgagtt tgaagtcgaa gttgcagagg
     4381 gtggaacggc taacttgaag gctgaaatca agtagtctcg gttttaacgg cgggatagct
     4441 cgctgactga ggggcagtgg ttcacatcgg accactgcct cttttgtttg gcaatcttcc
     4501 tcttcatcac atcaggtgtt ccggcctctg agtccatcga tttcatcagc atcatccagc
     4561 cggccaatcc gcgtcgttat tggttttcag cagttgttcc ctgcccgcca ctcgccttgc
     4621 gtttcccttt ctgaactggg taagatgcca aatttcaaca ccacattatc tgagtgggtg
     4681 atctgtgcct cgtgaattgt ctctcgccga agtgttccag ttagggtact actgggaaac
     4741 aaaaattctt ttgacagccg tgaagttaga cgtcttttcg gtgctggatg gacgcggccg
     4801 taccgcctct gaagcggccg agaaactcgg cgccgatgtg cgggcgctgg agttattgct
     4861 gaatgcgctg gtggcgatca ggcttttgtc gaagaccggc gatctctatg ccaatacgcc
     4921 ggtagccacg acgcatctcg tcaagcacgg gccgcaatat atcgggcact tgctgctgtt
     4981 gcatgatgcc gaatggggca attggggcaa gctggaggag gccgtgaaaa caggccgatc
     5041 gccggttacg cgacatgtct tcgagacgga tcccgcgctt ggcgcgaatg tgttgtccgt
     5101 cttgcaccgg atcgggcagc aaagtggccc agatttggcg aaacgccttg cgcttggcca
     5161 ggtacggacg atgctcgatc tcgggggcgg ggccggaacc aacgccattg cattttgtca
     5221 ggtgtacccg caactttcgg cgacggtctt cgatctgcaa acgacgctcc cattgaccga
     5281 acggaccgtg aaggacgcgg ggcttgaagg caggatttcc ctgaaggccg gtgacttcaa
     5341 ccgcgattcg ttgggtggcc cctatgacgt ggtgttgatg tccgatatcc ttcactatca
     5401 ggatctggca accaatgccg cgctggtgaa gaaaatctat agccatctga atcccggtgg
     5461 gcgcttgatc atcaaagacc ggttcttgga tgcctcgggt acgagccctg cctggacggc
     5521 ggcgtttgcc gtccatattc tcgtcaatac tgaacaaggc gcctgctacc gcaccgcaga
     5581 agccatgcag tggatgcacg atgggggata tgtctcggtt gaggaaatcg agcggacggc
     5641 ggtggtgcaa ggtgttcggc caagtgtcgg gtaagatgta ccatgtttga ttttttgcgt
     5701 cagccgggat tcttcggcac ccatgcgacg atgggggccg atctcagtca gttgatggcc
     5761 acgctgttca ccggcctctt catcatcgga tggttgcagg ccaagcagca tcgtggacat
     5821 caccatcatt ggctcatgtt gggcgggatg gtgaccatgg tcggattctt caccgcctac
     5881 tatctgtttc ggcagttggg ggtgttggcg ttcgaaggaa aagaaggatt cggggggtcc
     5941 caggccctgt acgactacgt gttcatccct gtgctgacaa tccatatcat tctggtgatc
     6001 atcgggttgg tcatggccgt gtacatgatt gtgctggggt tccgttcgca gcaatttctg
     6061 agcggtgcac gagtgctgag tgccgctcgg ctgctgacat cgtggaaaaa ggtgagcctg
     6121 atatttgcgg cgctcttggc ggtggtgatg ctgttattcg gaacacgcgt catgtctgcc
     6181 gggttttcca tgcgaaagct ggaggtctat atcgggtttc tcaccctggt cgcgatcgtg
     6241 tttgcagtgg agatgggcat tcaacggatc tggcctgatg gcgggcagcg gcaccgcgcc
     6301 ctcggccgtt tcaccatgat cgtctactgc atcctctttc tgaccggaac ctttacctat
     6361 gccatgttgt acatcctcta tcccgggaaa atcgggtagg aacgtcgagc ggagatgaag
     6421 agctgagggc cgatatgctt gtgtgcgggt gcggcggatg gatgcatacc gagggtgttg
     6481 aggagcgcat ctcccgtgag ggcgatccca cgtggtttgt ccgttcggag tgtcggccct
     6541 gccagtgggg agtgggggtc gatgtgccgg tgggacaggt cgatgggttg gtcgatcgtc
     6601 tgatgtggac tgatgatgcc ctgcatcggc tggatcgtgt tcctccctat gccgttccag
     6661 tcctgcgtga actggtggaa gggtttgccc ggaccagacg gcagcgagtg attacctacg
     6721 atttaatcga tcaggcgaaa acgggcgata tggtctcatg ggacctggat gcagagcagc
     6781 gcctcgccaa tgtgccggcg ccggttcggg ccatggcgcg cgttgagttg gaacgaacgg
     6841 cggtggatcg tggtgaatgc tctgtgaccg tcgcgctgat ggaagaagtg aaggcgcgat
     6901 atttcggcat ggcggctcag cgcgatgacg cgtaaatgct aaggcggctc ttgcgcggag
     6961 tccacggcgt cgctcccgcg ccccacatca ttgtgtttgg cgactgacaa catgttgcac
     7021 cgattggcat tacgggtcct tccaagtttg acgctggccg tcaccgaaga ttcggttcga
     7081 acaagaaagc gcctggtcat gtttgtcccg gggctggtgg cgtttgcggt gtaccgggcg
     7141 gcgaagcatg gcatgtcgct ggccgagccg gtgacgctgc tgctcctgag tggcctggtg
     7201 tcgctggtga cggcgatctg tgcctatcgc gtcggtcggg gggtgccgtt tgcgcaacta
     7261 gtgagcgcgg atggagccag acgtatcgga tggattcttg cctggatcgg ttttgtgtat
     7321 ggagtgcagt tgtcgctgct cgtgctggcg ctactctggc tcgttggata tgactatgcc
     7381 aagcatcccg atggcccggc catgatggcg atgattattc ccagtacggc ggtcgcgcgc
     7441 gatgcatttg agatcgggta tgtgcgatgg cttgaagcca gcgggcggcc gttcgtcacg
     7501 tttcctgatg gcgcagggct tcgtgccctg ctggcacggg tgacgccggc cctggcgggg
     7561 tggatggcgg caggggttgc cggttgtgtg agtctctcgc tgcttctggc ggcgctcgtt
     7621 agcggtgaca tggccgtgtt ggcgcaaggc gctgtcgtga cgatggcggc gggaacggtc
     7681 ggtctcctgg cgtttttcgc cgggcaagga aatggggcag cctggacggc cggattgtcc
     7741 gcgacccgct ggggtgaatt gatgaagttt tggtggtggc ccgggctggc cttcgcctcg
     7801 acctactatc tggtgttggt cggcggttgt ctgtatgtgt tgaaacagcc gatgagttca
     7861 cccggactct tcgctggcgt cgccggcctc gtcggtggcg tgatggcgct gtactgctat
     7921 tacctgggac atcggcggga tatagaaaat cgtcagcctg gaggagtgcc cagcgccttg
     7981 ttgcgctgcc cattcgtcat gggcattctc ggcaagtccc gcgaagccat tgctggtacg
     8041 gcattgagtg gatctgcggc tgtctttgac aagaacagcc agagagcgtc gtcatgatta
     8101 aacgccttcc gcttccgtcc gtgatcctgt tgttgctgtc cgggttgttg gtggccgcga
     8161 gtgttctgct gttgagtccg ccatcgctgg tggccgccga cccctccgca gatgtctatt
     8221 tccatacgcc tggagttccg gacggtcctt cggcgccgac ggcgaatgag aatcactatc
     8281 cgcaagtcgg gtcactcgat agccggttgc tgatgtggtt tatcatccag cagcatacct
     8341 acttcggtgg ttttgtcctg gccctaccga tcttttgcgt cctcttggaa tttctggggt
     8401 tggtcgcgag gaatcccgcg atggccgtgc gttatgacgg gttggcgcaa gatctgttga
     8461 aggtggcgct gctcgcgctt tccgtgaccg cagcggtggg gagcgtgatg ctgacgatgt
     8521 tcatcacgct gtaccccagc ttcatgcaat acatgggagg caccttcaag gtcatgatgc
     8581 ctctctatgc gctggtgttt gtcgggacga cctttcttac gatcgcgtac tactacagtt
     8641 gggatcgtat ggcagcgccg ggatcgaagt gggtccacct gtcgatcggc gtgctggcgg
     8701 tggtgttcgg cacgtcgctg ctgttattag ccaatgcctg gtccgccttc atgatggcgc
     8761 cgtccggcgt cgacgggcag ggacggtatt tggggaatcc ctggcattta ctccgctcgg
     8821 cactctggaa tcccctgaat ttgcatcgat tcctcgcgga catcatgtcg ggtggcgcgg
     8881 tcgtcctggc ctatgcctgt tatcggttct tttccggcaa gagtcaggag gagcgggctt
     8941 attacgattg ggtcggctat gtgttcttgt ttgtgacggt ctgcgccttg ttgccgatgc
     9001 cgttcgccgg ttattggttg atgcgttccg tctatgcgta cagtcaaagc atgggcgtca
     9061 cgatgatggg cgggttgctg acctggctct tcgtggtgca ggcgctgttg atcggtgccc
     9121 tctttcttgg cgtgaattac tatctctggc agagcatggg acggattcgc ggcggtgagc
     9181 gctatcagcc ctactatcaa tatctcctgc tcgcgctgac gggttgtctg tttatctggt
     9241 tgacgcctca caccattttg atgaccggga cggaagtcaa agccatgggc ggggcgcagc
     9301 atccggtcat cggaaactac ggcgtcatgt cgtcgaagaa cggcgcggtc aacgtgatga
     9361 tctgcatcac cgccctgagt tacatctttt atcgacgcgc aaatcggacg atgacgatct
     9421 catgggccaa agcgggaaat gtcatcctgg gggccctgtt cggtttcgga gcggcgcaca
     9481 tcatcggctt atcggtctat ggattttatc ttcccgccag tctccgcgtc gggttgtccg
     9541 gcccgcaggc cgtcaccacg ttgctcgtgg tcacgctggg tttgctgctg aaccgacgta
     9601 tgttgcgtgg tgcaacaatc cacggtcctg tacagtgggg ccagatttcg gttcgcggaa
     9661 tggttggctt gttcgggttg gcggcggcgt ttacctgggt gatggggttg atggggtata
     9721 tccgctcttc gggtcggttg gcttggcacg tcaacgagtt gatgccggat acgtccccct
     9781 gggcgtttac gccatcgctt ggattcgccg caaaaatggt cacgatcaac atggccctgt
     9841 tttggggggc ggtattcttc ctattttggg tctgtcagcg cgggcagcag ccggtgatgc
     9901 atgaagatgt gagcgcggag aaggaggcag gctttctgcc gtctccgtct catgagggat
     9961 aaggacggtg atgagggaat gtgagatggc tatgaagatg tttgtcgcgg caatagggct
    10021 gttgggtgtg gtgggattgt gcagtgtgat gggagttggc gctccggccc atgcaggcga
    10081 agccggcgtg ttggtgctgg aagattttca gtcggcagat caggacgggt ttccgatcga
    10141 ttggcagcat gagaaccagc gtagtcaggc caaggggcgc gatgcctaca agatccaatc
    10201 gcaagacggg aaaaaatttc tcgcggccaa ggatgcaggg cagcgtgtga agaaacgcaa
    10261 gatcgactgg gacccgaagg tctatccggt gctgacctgg cggtggcgtc tccataaggc
    10321 tcccagcggt tcggagccga ttgcggcact ctatgcttcg ctggatacgg atctcatgtt
    10381 tattccggtg tttacgaaat acatctggag cgccgggaag ccggaaggga cgttcgttga
    10441 gggcgggatg tttagcggat cagaattggt ggtgcagagc ggagtgcttc cggtcgggga
    10501 gtgggtggaa gagcgggtca acgtgtacga agattttaag cgtattcata agcacgagcc
    10561 ggctgagaag gcctggggta tttcgttgtt tgcggggccc ggtgtggaaa tcgattttgg
    10621 gacagttacg gtgagttcgg gcaagtgagt gttgggggaa ccccctttcg tagaaggtca
    10681 tcatggcagg gatgaatcca ccatcgacca gggtgttgct tgaagtggca tttggtgcgg
    10741 tggtgatggg tacggtcggt gcgttgatcg gagcaattct tggcggctcg tccattccat
    10801 tggcttcggg attgggattg gcgttgggcg cggtggttgg gtttctcggt gggcgccgct
    10861 tcctggtcag tatcctggtc ggtacggtac tcggtggcct gttggcgtgg tttattgccg
    10921 gaattgagaa ggtctcgttc ggggc
//
LOCUS       DAORQN010000040        10846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_343_length_10846_cov_12.384302, whole genome shotgun sequence.
ACCESSION   DAORQN010000040 DAORQN010000000
VERSION     DAORQN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_343_length_10846_cov_12.384302"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..811)
                     /gene="xrt"
                     /locus_tag="PK614_04190"
     CDS             complement(35..811)
                     /gene="xrt"
                     /locus_tag="PK614_04190"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HRB81439.1"
                     /translation="MTLLGLLTVALLGYMYADSLIFIFDQWGSDDYSHGLFVPIISVT
                     LIWQRRQRLVAIGIEPSWWGPPIVLTGLLLYIIGNLATVYIVTHVSLWLVIAGIVISF
                     IGPIATGKIAFPLFYLLTSIPLPDFLYNSLSSQLQLWSSALGVDFLQAIGVTAFREGN
                     VIDLGPVQLQVAEACSGIRYLFPLTTLALLCAYFLRDRMWKRVLLVLSAMPLSVFVNG
                     LRIGIVGLLVEFYGPQAAEGFLHLFEGWVLFLVTLGLVIG"
     gene            complement(984..1673)
                     /locus_tag="PK614_04195"
     CDS             complement(984..1673)
                     /locus_tag="PK614_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HRB81440.1"
                     /translation="MSQFKQRIHLRTVGLTLGAFCSLFAAITSEAANIPFNFSGSVAE
                     VHGGVFTSGGSGANGFGSALPVSGSFTFNSTTPDVLPGDPMWGLYANPIQNMTVKVGN
                     YTATFTPGSSVMQVINNPGLGDTFKVTLNGFTSSPATVSGLAPATFEMELVNPNGNVF
                     ANDHLPTTPPSLSSFASNQWRLVFGGVGNRVQGALTSLVPLPAAVWLFGAGLIALVGL
                     GSRGLVSRKES"
     gene            complement(2049..3281)
                     /locus_tag="PK614_04200"
     CDS             complement(2049..3281)
                     /locus_tag="PK614_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81441.1"
                     /translation="MHILFLSHYFPPEVNAPASRTFEHCRQWVRDGHRVTVVTCAPNH
                     PRGKIYPGYRNALYGREEKDGITIIRVWTYVTANEGFLKRTLNYVSYMGAAIMAAPFL
                     QKADIVISTSPQFFNGLAGYVVSRIKRAPWVLEIRDLWPESILAVGAIKNPFIIHLLE
                     WLELFAYRQADRIVPVTDAFQRYIEGKGIPKEKITVIKNGADLTLYKPVTGTNRLAES
                     LGVQGKFVAAYLGTHGMAHHLETVLYAAQRLREYRNIVFILAGDGAERQRLAALRNEL
                     GLDNVLMLDQQPKDQMPWLWALSDVSLILLKKSELFKTVIPSKIFESMAMGKPIILGV
                     EGESADIVRAADAGYCIEPENVEELVTRLIGLHSDKALCQEFGTKGRMHVSNYYNRIT
                     LAKRFEKLLEATHNEKCG"
     gene            complement(3374..4072)
                     /locus_tag="PK614_04205"
     CDS             complement(3374..4072)
                     /locus_tag="PK614_04205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB81442.1"
                     /translation="MDKWLYPIYSEKNWDDTILRDRILKILRRGDVMLDLGAGAGIIR
                     QMNFRGLAGRVCGIDLDPRILTQNRSLDEALQGSAEAIPYPSNTFDFVMANNVMEHIE
                     DPHGVYREVYRVLKPGGRFLFKTPNKWHYMPLIARLTPLWFHQYINRVRGRNTEDTFP
                     TKYLANSPRQIRALAKANGFRVESIELIEGRPEYTRLTALTYLFGFLYERIVNWVPGL
                     FVFRILIIGQLRKP"
     gene            complement(4201..5880)
                     /locus_tag="PK614_04210"
     CDS             complement(4201..5880)
                     /locus_tag="PK614_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRB81443.1"
                     /translation="MKRVLFYLTTLWYLRPTQILSLIRRRVLPASKPNSRISELRLRP
                     GVKLCGGIPASRVRAGDYSFCFLNQTKEFPDGQIDWTSREMSKLWRYNLHYFDYLLHA
                     DRSLDAKRKIIAAWISQNHPGTVDAWEPYTLSLRIVNWIKFFLQNSSYPKENSLHSLY
                     TQALWLEKNIEYHLLANHYFKNGVALFFAGMYFTGSDADRWLAKGREILASEIEEQFL
                     ADGGHYERSPMYHAISVTDCLDVLNLATSSCQAEMCSEMHRFKIRINKALEFLEDICL
                     PDGDIPLFNDSALGIAPHPRDILAYGNQITAYHRRLQANGLTIKSKSQTGYYVLRDGA
                     DMMVIDCGAVGPDYQPGHAHADTLSFELALNGQRVIVDSGVYDYELGPRRIYARSTCA
                     HNTIRIDGQDQSEVWGVFRVARRAYPLRSSLTSDQPRHATFEGAHDGYSRLPGCLIHK
                     RVIDYLSPGEWTVHDTVEGSGIHTIESYLHIHPSYSLHPGKKSVEIQNPLEQVILTIT
                     PALKVQIGIQPGSYFPEFGKELPNSTLVMTYRGELPITLTYRMSKTLPSNS"
     gene            complement(6035..7078)
                     /locus_tag="PK614_04215"
     CDS             complement(6035..7078)
                     /locus_tag="PK614_04215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81444.1"
                     /translation="MRLLLIGPIPPPLGGATVLFGQLVGELAQREDLQVEVVNTSRPH
                     RSISRNLLHGLRSLVALLRRIRHTEVVGLHASIQGAALFGPVVSVICYICRRPWIFRG
                     FGGFYPNWYQSAPVPIRWIFRHTVLGADIILFETRRSVEFFSSISSRPVRWYANSRAL
                     SSAPAVVNSCSDRGARDFVFLGHVSEIKGVHDLIHAARKLKGVTIDVYGPLTDRMTEA
                     EFTGSGVTYKGVIPPEAVPDMLRAYDVLVLPSRYWAEGYPGVILEAFSAGMPVIASSI
                     GAIPEIVDERNGILFEPGQVNQLINAIELLRDNPAQFTSLQLGAQEAALDFSSVRWTD
                     EFVQHCRKIYRSV"
     gene            complement(7063..7467)
                     /locus_tag="PK614_04220"
     CDS             complement(7063..7467)
                     /locus_tag="PK614_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRB81445.1"
                     /translation="MDYAIRLVFSCWLPHQVTAGENLILGYGGLAIVIHSDVIIGDNV
                     HIDQGVTLGGNGTEYGVPRIENNVYVGAGAKVLGPIRIGEGSVIGANAVVVDDVPPRS
                     LVAGVPAKIIRSGINIQTFLYQKKSKPLCDCS"
     gene            complement(7535..8701)
                     /locus_tag="PK614_04225"
     CDS             complement(7535..8701)
                     /locus_tag="PK614_04225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81446.1"
                     /translation="MLVHARRIKLAMVTPFPEANGLIKGGVESVSCNLIDSLRRIGSV
                     EIHVLSPSRKHAVTTEQRDGITIHWIPNSRLPGFLGYWSTFRRALHRQLHEIQPDLTH
                     FQGVAGWTLFYNRPYVFTIHGIPENDALFSKRWFSRSRSFLIGLIERLGRQRSPDTII
                     INPYVMTELKSQLRGRTWHIENPISREFFEIERKSFIPLILYVGRISHRKNILGLLRA
                     FHTVHEHIPNSRLHLAGSGDKQTLHACSIYVKESGLSTAVEFMGNVDRNTLTKELTQA
                     ACLALVSHQETAPMIIGEAMAAGVPIVASDMNGIPYMVEEGLTGFRVNQNDPHQIATQ
                     LIELLRNVPSNQVMGKRCREVAFKRFHPDEVVMQTLNVYRHILDQVRDSTASHA"
     gene            complement(8719..9840)
                     /locus_tag="PK614_04230"
     CDS             complement(8719..9840)
                     /locus_tag="PK614_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81447.1"
                     /translation="MRRETVLHLTGMHSTKYGGVERYLLGLTKSSARRGYHTAIQYES
                     QPQSAKYIQEIKQSGGEIIIARLGAGWVDSFFTLTHILRTMRPSIVLTHFVRGYIHLQ
                     LPFIKRRHGIRKILAMVHGIQSSRRSWRHFAFNRYDGIIGVSKGVSASLIAIGTRPEL
                     VSTHYLGLFRGNTRSQTERVRYRNEFGIKEDAIVIGCIAFDSKVKGVDLLLEAIARIS
                     SDFPQIHLLIIGIDARHSTLPAEAVRLGIGERTHWAGLREEGWRLLNAADLYAQPSRS
                     EAMPFAVMEAMELGLPVIATDVGGLPEIVADGETGYLARLDPAHLAEAINKMMAMRRS
                     WKTMGTAGHARYQKMFNGESSVESFIGIYFPSENQTVKG"
     gene            complement(9837..>10846)
                     /locus_tag="PK614_04235"
     CDS             complement(9837..>10846)
                     /locus_tag="PK614_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB81448.1"
                     /translation="FALALLLAGCVRNRDELNVITLYCLVGTVCGALVALYQHYTGNY
                     SVNVYEVQRAAGLRGDPNDTAMLLIAGIPLAIYWMLQCNSFVLKSLFAGTLVLLVLGI
                     ALTGSRGGFVTLLLVSLLIYAKRPTVLATISGLALATILLAFAPQSYWTRMETLISGQ
                     ELKTSGSMQHRSELQRKGLSILLEHPLLGVGPDNFGEAFLGTGRPRKVSVMTGGGRGK
                     NDPGLVAHNMYLEFFVENGIFGGLLLLAIFFKSMRSLMRYDRNESQGSGYFGIGNALA
                     LALLGMLFSGLFLSQGKNSVLWFLVGIGFATGQVMNSALRASPCEFNRQDHSPDAIGM
                     A"
BASE COUNT         2802 a         2747 c         2626 g         2671 t
ORIGIN      
        1 tgcgctagca ggtggagacc ctatcacact ctacttagcc tattaccagt cccaaggtca
       61 ccagaaacag gacccagccc tcaaataggt gtaaaaatcc ctcagccgcc tggggaccgt
      121 aaaactccac aagcagaccg acgataccga tccgaagtcc gttcacaaac actgataaag
      181 gcatggcaga tagcacgagc agtactcgct tccacattcg atccctgaga aagtatgcgc
      241 ataagagagc gagtgtcgtg agggggaaca gataccggat gccactgcag gcttcggcaa
      301 cttgcaattg aactggaccg agatcaataa cgttcccctc acgaaatgcc gtcaccccga
      361 tggcctgaag aaaatctacg cccagggcag atgaccataa ctgcaattgg ctggagagcg
      421 agttataaag aaagtccgga agaggaatac ttgtgagtaa gtagaataaa ggaaaagcaa
      481 tttttccggt agcaatcggc ccaatgaatg aaataacaat gcctgcgatc accaaccaga
      541 gtgaaacatg agtgactata tatacagtcg ccaggttgcc gatgatgtag agcaacagtc
      601 cagtcagaac gatgggcggt ccccaccagg aaggctctat accgatggcc accagtcgtt
      661 gccgccgttg ccaaataagg gtgacgctga taatcgggac aaagagtcca tggctatagt
      721 cgtcactccc ccattggtcg aaaatgaaga tcaggctgtc ggcgtacatg tagcccaaga
      781 gggcaacagt gagtaacccg agaagagtca ctggacacct ggatgtaaat ctagtcctct
      841 cccccgattg ggggagagga aaagagccgg agagcgacgt gagtcgtgct aatgctgcag
      901 ggagtagcgt gatcgttaag cgtcgaggtt aaagagctta tgtctggaaa tgtcaaagac
      961 agttgttcag gtctgtccca ggtttacgac tcttttcgtg ataccaggcc gcgcgatcct
     1021 aatccgacca gtgcaatcaa gcctgcaccg aacagccaaa ctgccgcagg caatggaacg
     1081 agagatgtta atgctccctg tacccggtta cctactccac caaaaaccaa acgccattga
     1141 tttgaggcaa aagatgagag acttggagga gtggtaggaa ggtggtcatt cgcgaagacg
     1201 tttccattag gatttaccaa ctccatttcg aacgtcgcgg gtgccaggcc gcttaccgtt
     1261 gcaggactgc tcgtgaatcc attgagcgtc accttaaacg tgtcaccgag gccggggttg
     1321 ttaatcactt gcatgacact agatccaggg gtgaaggttg ctgtgtagtt cccaacctta
     1381 acggtcatgt tctggattgg gttagcgtac aggccccaca ttggatctcc aggcaagacg
     1441 tccggagtgg tggagttaaa ggtgaaactt ccagacaccg ggagcgctga gccaaaacca
     1501 ttagccccgg atcctcccga tgtaaagaca ccaccgtgaa cttccgccac cgagccgcta
     1561 aaattgaagg ggatgttcgc cgcctcgctt gtgatagctg caaatagaga gcaaaaagcg
     1621 cccagcgtca gtcctacagt tcgaaggtgg attctttgtt tgaactggct catcgtttct
     1681 cctccacgaa gaaaagtaaa cagtacagct atgcttcgat aagcagcata gagttaattt
     1741 actcgactac ttatcatgaa tattttttgc acaatactta aaaccaataa actgcgcaag
     1801 cgttttttct ggaaatcaga aaatcggata agtagatcca tgaagtgaat ggaaatacct
     1861 tcagtgtgta gcgttctgat acgaaaatcg aaatcgaatg tataaacttg ttactgtggt
     1921 aagcaacgca tatatcactg gagcacaagc attaggccca aaaggatatg tccattcggc
     1981 accatcggaa tccgcaccgg ttgagaattt cattttcacc ttcgagtgat cccaggtgag
     2041 gtgcgtattt acccacactt ttcgttatga gttgcttcca ataatttctc gaaacgtttt
     2101 gccagtgtga tgcgattata atagttgcta acgtgcatgc ggccctttgt gccaaattct
     2161 tggcagagtg ctttgtcact gtgcagtcct attagacgag taacaagttc ctcgacgttt
     2221 tctggctcaa tgcagtagcc ggcgtccgca gcacggacaa tgtcagcgct ctctccttcg
     2281 accccgagga taatcggctt ccccatggcc atactctcaa aaatcttcga gggaatgacg
     2341 gtcttgaaga gttcggattt cttcagcaag atgagactca catcggatag cgcccagagc
     2401 caaggcatct gatctttcgg ttgttggtcc agcatgagga cattgtcgag accaagttca
     2461 ttgcgcaagg ccgctagccg ctggcgctcg gcaccgtctc cggcgagaat gaaaacgatg
     2521 ttccggtatt cacgcaggcg ttgcgccgca tagagcaccg tttcaaggtg gtgggccatt
     2581 ccatgcgtgc ctaaataggc ggccacgaac ttcccctgaa ctccaagcga ttcagccagt
     2641 ctgtttgtgc cggtgaccgg cttatacaag gttaggtctg ccccgttctt gatcacggtt
     2701 atcttctctt tggggatccc ctttccctcg atgtaccgtt ggaacgcatc cgtgaccggc
     2761 acgattcgat cggcctgacg gtaggcaaac agttccagcc attccaggag atgtatgatg
     2821 aagggatttt tgattgcacc caccgctaaa atggattccg gccagagatc ccggatttcc
     2881 agcacccagg gggcccgctt aattcggcta accacatacc ctgccaggcc gttgaaaaac
     2941 tgaggcgagg tggagatcac gatatcggcc ttctgaagga acggcgcagc cataatggcc
     3001 gcccccatgt aggagacgta gttcagtgtc cgtttgagaa atccttcatt ggcggttacg
     3061 tacgtccaga cacgaatgat cgtgatcccg tctttctctt cacgcccata caacgcgttg
     3121 cgatagcccg gatagatctt tccccgaggg tggttcggtg cacaggtgac gacggtcacg
     3181 cgatgcccgt cccgcaccca ctgtcggcaa tgttcgaatg tgcgcgaggc cggcgcattc
     3241 acttctggcg gaaaataatg agacagaaaa agaatatgca caggctcaac tcatccttct
     3301 tgcgtggcaa acaaatgaga tccttcttcg cacggctgca aatgattttc gctcatggca
     3361 tcgcgcgacg catctaaggc tttcgaagtt gacctatgat taggattctg aagacaaaca
     3421 agccaggtac ccagttcaca atacgctcat aaagaaatcc gaataaataa gttagcgccg
     3481 tcagcctggt gtattcgggc ctgccttcga tgagttcgat cgactccacg cggaacccat
     3541 ttgccttggc tagcgccctg atctgccttg ggctgttcgc aagatattta gtcgggaatg
     3601 tgtcttcggt atttctgcct cgaacacggt taatgtactg atgaaaccat agcggcgtaa
     3661 gtcttgcgat gaggggcata tagtgccatt tgttcggtgt cttaaataag aatcgacctc
     3721 cgggtttcag cacacggtaa acttctcgat agactccatg aggatcctct atatgctcca
     3781 ttacgttatt tgccatgacg aaatcaaagg tgttactcgg ataaggaatc gcttcagcac
     3841 ttccctgaag agcctcgtca agggagcgat tttgagtcag gattcgcgga tctagatcaa
     3901 tcccgcagac gcgcccagcc aatcccctga agttcatctg gcggataata cctgccccag
     3961 caccaagatc gagcatcacg tctccacgcc tcagtatctt gaggatgcgg tcccgtaata
     4021 tcgtatcgtc ccaatttttt tccgaatata tagggtatag ccacttgtcc atcctcgcca
     4081 caaggttact catgtttccc ccatgcagta attctgccgt gacaaaatcg cgaagcccaa
     4141 atcactaggc gtattgaagc cctgaaagta tccttctcat gcggccattc aattcttgaa
     4201 tcatgagttc gacggcagtg tcttgctcat acggtatgtt agggtgatgg gtaattcacc
     4261 gcgataggtc atgaccagag tgctattggg taactccttt ccgaattccg ggaaatagga
     4321 tcccggctgg atccctatct gtacttttag ggctggtgta atagtaagaa tcacctgttc
     4381 tagaggattt tgtatctcca cagatttctt gcctgggtgc aggctgtatg agggatggat
     4441 gtggagataa ctctcaatcg tgtgtattcc gcttccctca accgtatcgt gaacggtcca
     4501 ctctcccggc gagaggtagt cgatgactcg cttatgaatg aggcatccag gcaacctgct
     4561 atatccgtca tgggccccct caaaagttgc atgtctcggt tggtcagatg tcaaactgct
     4621 acgcagtggg tatgctcgtc gtgcgacccg aaagactccc cacacctcgg attggtcctg
     4681 accatcaatt cggatcgtgt tgtgtgcaca agtgcttcga gcatagattc tccgagggcc
     4741 cagctcataa tcgtaaaccc cactatccac gatgacgcgc tggccattca gagccagttc
     4801 aaaactcaag gtatccgcat gtgcatgacc gggttgataa tccggcccta ctgctccgca
     4861 gtcaatgacc atcatgtccg ccccatcgcg gagaacgtaa tagcctgttt gggatttact
     4921 ttttatcgta agaccattgg cttgcaatcg tcgatggtaa gcggtgattt gattgccata
     4981 cgcgagaata tctcttggat gcggtgcaat acctaaggct gaatcgttga ataagggaat
     5041 gtcgccgtca ggcagacaaa tgtcctcaag aaattccaac gccttgttga tgcgaatttt
     5101 gaaccgatgc atctcggagc acatctcggc ttgacatgac gacgtcgcga ggtttagcac
     5161 gtccaaacaa tctgtcacac taattgcatg gtacatgggg ctgcgctcgt aatggccgcc
     5221 gtctgcaaga aattgttcct ctatctcact ggcaagaatt tctctcccct tcgctagcca
     5281 acgatccgcg tctgagcccg taaagtacat gccagcgaag aatagcgcca ctccgttttt
     5341 aaaatagtgg tttgcaagca agtgatactc gatgtttttt tccaaccata gcgcttgagt
     5401 ataaagactg tgtagcgagt tttcctttgg gtaggatgaa ttctgcagga aaaacttgat
     5461 ccagttaaca attcgcaaag aaagtgtgta aggctcccat gcatccacgg tgcccggatg
     5521 gttttggcta atccaggcag cgattatttt tctctttgca tcaagcgagc gatccgcatg
     5581 aagcaaatag tcaaaatagt gcaggttata acgccaaagt ttcgacatct ccctgctggt
     5641 ccaatcaatc tgaccatcag ggaactcttt cgtctgattt aagaagcaga atgaatagtc
     5701 tccagctctc acgcgcgaag cgggaattcc accacagagc tttacaccgg gccgtagtcg
     5761 gagttcagat atcctagagt ttggcttgga tgctggtaaa actcgtcttc gtatgagcga
     5821 cagtatctgt gtggggcgta gataccaaag cgttgtgaga tagaacagca ctcttttcat
     5881 gatcattgtg cattatcgct gctgcctggc ccttccatga gcatgttgag acacgatttg
     5941 gatcgctgct aaatttcgcg cctgaaaggt ccacgcctgc tccatcaagg agcgcttgtc
     6001 aacgcagata gccaggagaa tcgctcggga aatgtcacac gcttcgatag atttttcgac
     6061 aatgttgcac aaactcatcg gtccatctaa cagaagagaa gtcaagtgcc gcttcttgag
     6121 cgccgagttg cagtgaagtg aactgcgctg ggttatcgcg aagtagttct atggcattaa
     6181 tgagctggtt aacttgccct ggttcaaaga gaattccatt acgctcgtcg acaatttccg
     6241 gtatcgcacc gatcgatgac gcaataaccg gcattccagc gctgaatgct tccagaatga
     6301 cgccaggata accttcagcc caataccggc ttggcagaac caaaacatca taagcacgca
     6361 acatgtcagg caccgcctcc ggtgggatca ctcccttata ggtaacgccg cttccagtaa
     6421 attctgcctc ggtcattcgg tcagtcagtg ggccataaac atcgatggtg acgcccttga
     6481 gcttacgcgc agcgtgaatc aaatcatgga caccttttat ctcgctgacg tggcctagga
     6541 agacgaaatc acgcgctccc cgatcgctac acgaattaac cacggctggt gcggatgaaa
     6601 gagcccgact attagcgtac cagcgtacag gtcgactgga aatggagctg aagaactcaa
     6661 ccgaacggcg agtctcaaac aaaataatgt ctgccccaag tactgtatga cgaaagatcc
     6721 aacgaatcgg cactggggca gactggtacc agttagggta aaaccctcca aaccctcgaa
     6781 agatccaggg ccgtctacat atgtaacaga ttactgaaac aactggcccg aacagtgcag
     6841 caccctgaat cgaggcgtgg agtccaacga cctctgtatg tcgaatgcgt cgcagcagcg
     6901 ctactaggct tcgcaatccg tgtaggagat ttctgctaat actccggtga gggcgagatg
     6961 tgttgaccac ttcaacttgc agatcttctc gctgcgctag ttcgccaacc agctgaccaa
     7021 ataaaacggt tgcgccgcca agaggcggag gaatagggcc aattaagagc aatcgcataa
     7081 aggcttagac tttttttgat aaagaaacgt ttgtatatta atgccgcttc ttatgatttt
     7141 tgccggcact ccagcaacaa gggaacgagg cggaacatcg tcaactacga ctgcattggc
     7201 cccaattaca gacccctctc cgatcctaat cggaccgaga actttcgctc cagcaccaac
     7261 gtacacattg ttctcgattc tcggaacacc gtattctgtt ccgttaccgc ccagggttac
     7321 tccctggtcg atgtggacat tgtctccgat aattacatca ctatggataa caatggccag
     7381 accaccatat ccaagaatta agttttctcc agccgtaact tggtgaggca gccaacacga
     7441 aaacaccagt cttatcgcgt agtccataag tctcgccaaa aggggcatgc ggtgtacggc
     7501 taaccaccgc gccaggcgat gccacgttac gatgttacgc atgtgaggca gttgaatccc
     7561 gtacctggtc tagtatgtgt cgatagacat ttagagtctg catcaccact tcatctggat
     7621 gaaatcgttt gaaagcgact tctcgacagc gtttacccat tacttggttg ctaggcacat
     7681 tgcgaagtag ttcaataagt tgggtcgcga tctggtgcgg atcattttga tttacccgaa
     7741 atcctgtcag accttcttca accatataag gtatgccgtt catatctgaa gcaacaatgg
     7801 ggacaccagc agccattgct tctccgatta tcattggcgc cgtttcttgg tgagacacta
     7861 atgcaaggca agccgcttga gtgagttctt ttgtaagagt attcctgtcg acattaccca
     7921 tgaattctac ggctgtactc agtccggact ctttcacata tatcgagcaa gcatgcaagg
     7981 tttgtttgtc gccgcttcct gcgagatgga ggcgagagtt tggaatatgc tcgtgaactg
     8041 tatggaaagc gcgaagtagt ccgaggatgt tctttcgatg actaatacgg cctacgtaca
     8101 atataagtgg aatgaatgat tttctctcaa tttcaaagaa ctctctggag atagggtttt
     8161 ctatgtgcca tgtccgtcct cgaagttgag attttaattc cgtcattaca tacggattga
     8221 tgatgatggt atctggcgat cgctgtctcc ccagcctctc gatgaggcct atgaggaagc
     8281 tgcgcgatct gctaaaccag cgtttcgaga agagggcatc gttttctgga attccatgaa
     8341 ttgtaaatac gtaaggacga ttgtagaaaa gagtccagcc tgctactccc tgaaaatgtg
     8401 ttaggtcggg ttgaatttcg tgaagttgac ggtgaagggc ccttcgaaag gtactccaat
     8461 atccaagaaa tcctggcaga cgagaattgg gaatccagtg aatcgttatt ccgtcccgtt
     8521 gttcggtggt tacagcgtgt tttcggctag gagaaagaac atgaatctcg actgatccaa
     8581 tacgacgtaa actatcaata agattgcagg agacgctttc tactcctccc ttaatgagtc
     8641 cgttcgcttc aggaaatggt gtgaccatcg ccagcttgat tcgacgagcg tgtacaagca
     8701 tgtgctctgc tagtttcctc atcctttaac ggtttggttc tcagagggga aataaatccc
     8761 aataaatgat tccaccgatg attccccgtt aaacattttt tgatacctgg cgtgccccgc
     8821 cgtacccata gttttccaac ttctcctcat cgccatcatt ttatttattg cctcagctaa
     8881 atgggcgggg tccagtctag ccaagtatcc agtctctccg tccgccacta tctcagggag
     8941 accgccaaca tctgtcgcta tgactggcaa tccaagttcc atggcctcca taacagcgaa
     9001 tggcattgct tctgagcgtg aaggttgggc atagagatct gctgcattta agagccgcca
     9061 accttcctct ctcaacccag cccagtgagt ccgttcgccg ataccgagcc gaaccgcttc
     9121 ggcgggcagg gttgaatgcc gggcatcaat tccaataatg agaaggtgga tttgggggaa
     9181 atcactcgaa attctggcga tggcttccag tagaaggtca acccccttaa ccttggagtc
     9241 aaaagcgata cacccaataa cgatcgcgtc ctctttaatc ccaaattcat ttctatatcg
     9301 cacacgttcc gtttgcgatc tggtatttcc cctgaacaga cctaaatagt gagtgctaac
     9361 gagctctgga cgagttccaa ttgctattag gctggccgaa acaccttttg aaacgcctat
     9421 gatgccgtca tatcgattga aggcaaaatg cctccacgac cttctagagc tctggatgcc
     9481 atgcaccatc gccaggattt tcctaatccc atgcctacgt ttgatgaagg gcaactggag
     9541 atgaatatag cctctgacga agtgagtcag gacaatagat ggcctcatgg ttcgaagaat
     9601 atgggtgaga gtgaaaaatg agtccaccca accagcacca aggcgagcga taattatttc
     9661 gccaccggat tgttttatct cctgaatgta ttttgcactt tgaggctgtg actcatattg
     9721 gatagcagta tgatagccgc gtcgggccga agattttgtc aacccgagta agtagcgttc
     9781 gacccctcca tatttcgtgc tatgcatccc tgtcaaatgc agaactgttt ctcgtctcat
     9841 gccattccaa ttgcgtcagg cgagtggtct tgcctgttga attcgcaggg tgacgctctg
     9901 agtgctgaat tcatcacttg tcccgtggca aacccgattc ccacgagaaa ccacaataca
     9961 gaattcttgc cttgagatag aaacagacct gagaacagca tccccaacaa ggccagggcg
    10021 agagcattcc caattccgaa ataaccactg ccctgagact cgtttcggtc atacctcatc
    10081 aagctgcgca ttgacttgaa aaagattgca agtagcaaga gaccgccaaa gatgccgttc
    10141 tcgacaaaga attcaagata catattatgg gctactaacc ctggatcgtt ttttcctcgc
    10201 cctccccctg tcatgactga aaccttcctc ggccggcctg ttccaagaaa ggcttctcca
    10261 aagttatctg ggccaacccc caagagcgga tgctctaaca aaattgataa ccccttacgt
    10321 tggagttcgc ttcggtgttg catggaacca ctggtcttca gctcttgacc gctaattaat
    10381 gtctccatcc gagtccaata agattgaggc gcaaaggcta ataagattgt ggcgagggca
    10441 agcccagaaa tcgttgctag aactgtgggt ctcttcgcgt agattaagag agagaccaac
    10501 agtaaggtca cgaagccgcc tcgagaccct gtgagcgcta taccaagcac aaggaggacg
    10561 agcgtccccg cgaacaggga ttttagtacg aatgaattgc actgcagcat ccagtatata
    10621 gccaggggta tgccagcaat taatagcatt gctgtatcgt tgggatcccc ccgtaatcct
    10681 gcagcccgtt gaacttcata gacattgacg gagtagtttc ccgtgtagtg ttggtaaaga
    10741 gcaacgagag caccacaaac cgttccgacc aaacagtaaa gtgtgattac gttcaactca
    10801 tctctatttc ttacgcagcc cgctaacagc aaggcaagcg cgaagg
//
LOCUS       DAORQN010000041        10638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_349_length_10638_cov_11.060191, whole genome shotgun sequence.
ACCESSION   DAORQN010000041 DAORQN010000000
VERSION     DAORQN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_10638_cov_11.060191"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK614_04240"
     CDS             <1..728
                     /locus_tag="PK614_04240"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRB81449.1"
                     /translation="VPYAHAVAFGHARRGRLVVNIGGISNVTYLPPGGGVAELRAFDT
                     GPGNMVLDAIVHEATKGQSAYDAGGRRARKGTVNRPLLTELMAHPFLTRRPPKSTGRE
                     EFGAPFVRTLLAKQRRARLSVEDLLATCAAWTAEAIGSSRRWVTGEIHDVIVGGGGVY
                     NRAIMASLRQVFAPTSVLTFDECGWSSKAFEATAFALLAHDTYHGQCTNVPQVTGAHH
                     PVLLGAVVPGSPHMLTKWARLAR"
     gene            744..1262
                     /locus_tag="PK614_04245"
     CDS             744..1262
                     /locus_tag="PK614_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRB81450.1"
                     /translation="MELLYPILGIGALVFLGSLAWESFGRRRSPGASMAEAPAGMSFK
                     AKPLLTDQEVQLYNLLRLAVEDRYLLFTQIPLWSLLDLRLPSGTPPAEMLRELALKRA
                     TFVLVHPGSRLVEKVVQVERPVPDDAPDGSHDSLFESALRAAGVQLVRLSPLHAYTVP
                     GLVTVLDLADPE"
     gene            complement(1313..1834)
                     /gene="ybgF"
                     /locus_tag="PK614_04250"
     CDS             complement(1313..1834)
                     /gene="ybgF"
                     /locus_tag="PK614_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRB81451.1"
                     /translation="MTRTHTKLGPVRGICLILALIGNLTTVPVLFAASPQPPDDAHRL
                     YDRVMEEFRHKDYAAALAGFRFFLELHGQSSLSANAQYWKGECQYRMGRYKDALDSFY
                     NLISDYPMSQKLAASTLKIGQIYTKQGDREKAQMMFERVTDQYPDSTEAEVARKALEA
                     AAAKGEPVAAEPS"
     gene            2158..2991
                     /locus_tag="PK614_04255"
     CDS             2158..2991
                     /locus_tag="PK614_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRB81452.1"
                     /translation="MERPQESGAVGGRREPLSEPPPNPKGGRTLIVDARDPHAYIRPS
                     AALKEAGETDQVFIRPGVYEDKVFVADRPVLLIGAGRDHVQIYSRRGGPLYLQRVPSG
                     MISGITFRYVGSDQHSAINVLDSVCTITQCRATEGVLSGVVIYGPEARATFVENEVCY
                     NRESGIFVFAGAQPRVAQNQCYGNHHFGLAVRDPGSHPECVRNVCHSNMLSGILLFHH
                     AEALLLDNTCRDNQHWGLVMTPDTHPTPPREDLVTSNVLLPNPRGSLVVTEQPLADIG
                     R"
     gene            complement(3071..4003)
                     /locus_tag="PK614_04260"
     CDS             complement(3071..4003)
                     /locus_tag="PK614_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HRB81453.1"
                     /translation="MIKLLLVEDNPVDAQLTRDLLAEWPLDQFEITHAPILAEGLTRL
                     SRDRFDVVLLDLSLPDTHGLSTVTQVLATSPGVPVVVLSGHDDHPLALKALQHGAQDY
                     LVKGEGGTDFLARSILYAIERKRAQERLTYLAQHDQLTGLINRALFRDRLIHAMARSK
                     RKDHPLAIMLLDLDRFKAVNDTLGHDVGDQLLKLVATRLLECVREVDTVARMGGDEFT
                     AILEGISGVADVLVVAKRIVESISTPFEIGPHRISIGVSIGITLYPLDDEDIDELLRH
                     ADKAMYAAKQQGGSRFHFHSTTGQPPSGTPAPVQ"
     gene            4638..5411
                     /locus_tag="PK614_04265"
     CDS             4638..5411
                     /locus_tag="PK614_04265"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB81454.1"
                     /translation="MDHEKAVRQELRVVSHNLGGQSDDGGRVTAMWALPLALAGVLVA
                     GEPASADSEIYGYVGPNGVFEMTNVPTDQRFRSAESQVRRLSHRASAEEVEEAVKRYA
                     FQFQLHPALLLAVIKAESDFNPTVISRSGAVGLMQLIPETAIRHGVLNLYDTDDNIRG
                     GARHLRYLLDRFNGNVRLAVAAYNAGERRVERYRAIPPYPETRDYVRKVMIYYKSFRG
                     DYQASPGKAVLLAMYHKPLQLEPLPVSTDVRNSRPAVRK"
     gene            6089..7201
                     /locus_tag="PK614_04270"
     CDS             6089..7201
                     /locus_tag="PK614_04270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81455.1"
                     /translation="MISVFGSCVGQEEIDEIRTSFEKQWIGIGLKTRSFESLFAHRLG
                     LKDFVFLDNGSNSLYMAVKLLNLSPGSEVILPSFTWVACAHAIILAGGIPVFCDVDVN
                     TQNVTADTIAPHISSKTRAIMVVHYAGKPVRMEPVLSFGQPVIEDAAQAVDSKLGNKY
                     CGSIGTIGVYSFDPVKNITTGEGGGITTRDSELMTRARQLRYCGIGSAGFEAAAMKER
                     WWEYDIVDVFPKLLPNDIAASIGLAQLRKIDKFQTIRKKIWNIYQAQLADFDWLIRPQ
                     DPASDEQHSYFTYSIRVAKGKRDQLAKYLYSRGIYTTVRFHPLHLNPIYKSSYRLPIC
                     EQLNEEILSLPLHPNLSDSDIDAILLSLQKFNEQYM"
     gene            7533..8426
                     /locus_tag="PK614_04275"
     CDS             7533..8426
                     /locus_tag="PK614_04275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81456.1"
                     /translation="MPRVSVIIPAYNSMQWVSAAIDSVLSQGYRDYEVVVIDDGSTDK
                     TADVLESYGRKIRCIYQANAGVSNARNRGLSVSTGEFIAYLDADDMWYSQKLECQIAF
                     LDAHPQCGLVHSDVTVIDEQEKVIRARFNVETARPVPSGYCLQDLLRRCHIQVPSVIE
                     RRECIEEVGGFDEQFMATEDYLHWIMVSLKGWEFGYINEPLAKYRWRGGSVSSNKRLF
                     LEEYGKMFGVLLPALYENRPQAPDLAIIRKRFLQVERELAYMDCIEGHLSSARQRLMR
                     LIQRSPFRYNLYWDLAKTYLR"
     gene            complement(9624..10268)
                     /locus_tag="PK614_04280"
     CDS             complement(9624..10268)
                     /locus_tag="PK614_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81457.1"
                     /translation="MTKPRVLLADDHSLVLEGFRRILEDQCELVGMVEDGRALLEAAR
                     KLEPDIVILDVSMPLLNGIDAATQLKKVQPSVKVIFVTMHSDADYVRSAFESGASGYL
                     LKRSAVDELEQAIRAVWAGHTYITPLIAKDLLDVLLTTGSRQPQQKKTLTFRQREVLQ
                     LLAEGRTMKDIALRLKISTRTVEFHKAQVMEQLNLRTTADLIKYALTHGVLASS"
     gene            complement(10261..>10638)
                     /locus_tag="PK614_04285"
     CDS             complement(10261..>10638)
                     /locus_tag="PK614_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81458.1"
                     /translation="VDDFSASTGLEAVYVHRDPVSPVPTDLATCVYRIAQESLTNVAR
                     HAHASEVEVELICDEGMITLSIRDNGIGFDLSRASQERGRLGLLSMKERVRLVRGTLD
                     VIAAQGCGTHVEVHVPLSGSNHD"
BASE COUNT         2262 a         2825 c         3112 g         2439 t
ORIGIN      
        1 tggtccccta cgcccatgcg gtggcctttg gccatgcgcg tcggggtcgt ctggtggtga
       61 atatcggcgg aatcagtaac gtgacctatc tgccgccggg cggtggtgtc gccgaactcc
      121 gtgcgttcga caccggccct ggtaacatgg tgcttgatgc gatcgtgcat gaagcgacga
      181 aggggcagag tgcctacgat gccggcgggc ggcgcgcacg caaggggaca gtgaaccggc
      241 ctctgctcac cgagttgatg gcccatccgt tcctgacgcg gcgtccgccc aagtcgaccg
      301 gccgggaaga gtttggtgcg ccgtttgttc gaacgcttct cgcgaaacaa cgcagggcgc
      361 gtctgtccgt cgaggatctt ctggcgacct gtgcggcctg gacggcggag gccatcggtt
      421 cgtcccgacg gtgggtcacc ggcgagatcc atgatgtgat cgtcggaggg ggcggtgtgt
      481 acaatcgcgc cattatggcc tctctccggc aggtatttgc gccgacgtct gtgttgacgt
      541 ttgacgaatg tggatggagc agcaaggcct ttgaagcgac ggcgttcgcc ctgctggctc
      601 acgacaccta tcacggacaa tgcaccaacg tgccgcaggt gacgggggca catcaccccg
      661 tgctccttgg cgctgtggtt ccgggatccc ctcacatgct gacgaaatgg gcgcgtctcg
      721 cccgctgatc cgattggctt gtcatggaat tgctgtatcc tatccttggt atcggggcgc
      781 tggtgtttct gggaagcctg gcctgggaga gtttcgggcg gcgccgttca cctggtgcgt
      841 ccatggcaga ggctcctgcc ggtatgagtt ttaaggccaa gccgttgctg accgaccagg
      901 aagtgcagct ctacaatctg ctgcgcctcg ccgtggaaga ccggtacctg ctgttcacgc
      961 agattcctct ttggagcttg ttggatttgc gtcttccctc gggaacgccg cctgccgaga
     1021 tgttgcggga actggcgctc aaacgcgcca ccttcgtgct ggtgcatccg ggcagccgtt
     1081 tggtcgagaa ggtggtacaa gtcgagcggc cggtgccgga tgatgccccg gacgggtcgc
     1141 acgattcact ctttgagtcg gcgcttcgtg cggcaggggt gcaactcgtg cggctgagcc
     1201 cgctgcatgc ctatacggtt cccggtctcg ttaccgtgtt ggatcttgcc gatccggaat
     1261 aacgccggtt cgactgccaa cgggcctgtg tcgaaagggc gcgcaatccg gatcagcttg
     1321 gctcggcagc gaccggttca ccctttgcgg cagcggcctc caaggctttg cgagccacct
     1381 cggcctctgt gctatcgggg tactggtccg tcacccgctc gaacatcatc tgcgcttttt
     1441 cgcggtcgcc ttgcttcgtg tagatctgcc cgatcttgag cgtcgaggcg gcgagcttct
     1501 ggctcatcgg gtaatcggag atgaggttgt agaacgagtc cagcgcgtct ttgtagcgcc
     1561 ccatacggta ctggcactcg cctttccagt attgagcgtt ggcggacagg gacgattgcc
     1621 cgtggagttc gaggaagaag cgaaacccgg cgagggctgc ggcatagtcc ttgtggcgga
     1681 actcttccat cacgcgatcg tagagccggt gtgcgtcatc cggcggttga ggtgaagctg
     1741 cgaacagaac tggaacggtc gtgaggttgc cgatcaacgc caagatgaga cagatgcctc
     1801 gcacgggacc gagcttggta tgagtcctgg tcatgagtgc ccctctttct tccctgttgg
     1861 ccgagtctgg gttcacacgg tcgttgtgcc ttccgatatg aaagaaccgc accagcgagg
     1921 ctcgacaagt gaacccgttg tgaaattgta tagttcgcgt ggaattgaag gcggagtggc
     1981 ccggatgtgc cggaacaact ggggcggaaa gcagacagca ttgtacggaa agcgccagcc
     2041 atgaggcggt gctctggccg gttgacagat cgcatgtttt cacttgttgt cggttagcag
     2101 gcagtcggcc atggcggcgg gtgtgctgat tccgagtctg tagaggatcg agaaagtatg
     2161 gaaagaccac aggagtcagg cgcggtcggt ggccgaaggg agccgctcag cgagccacct
     2221 cccaatccga aggggggccg gacgttgatt gtcgacgccc gcgatcccca cgcctacatc
     2281 aggccgagcg cggcactgaa agaggccggt gagactgatc aggtcttcat tcgaccggga
     2341 gtgtatgaag acaaagtctt tgtcgcggac cgtcctgtgc tcctcatcgg cgccgggcgt
     2401 gatcacgtgc agatttacag ccgtcgcgga gggccgctct atctccagcg ggtcccctcg
     2461 ggcatgatca gcgggattac cttccgctat gtgggcagtg atcaacattc cgcgatcaac
     2521 gtcctggact cagtctgcac gattacgcaa tgccgggcga cggaaggcgt gttgtcagga
     2581 gtggtgattt atggtcctga ggcccgtgcg acgtttgtcg agaatgaggt ctgttacaac
     2641 cgcgagtcag gcatttttgt ttttgcgggc gcacaaccgc gtgtggcgca aaaccagtgt
     2701 tatgggaatc atcatttcgg cttggccgtg cgagatcccg gcagccatcc ggaatgtgtg
     2761 cgcaacgtct gtcacagcaa catgctcagc gggatcctcc tgttccacca tgcggaggcg
     2821 ctcctgctcg acaacacctg tcgggacaac cagcactggg gtttggtcat gaccccggat
     2881 acgcatccga ctccgcctcg cgaggacttg gtcacctcta atgtgttgct gccgaatccc
     2941 cgagggtcgt tggtcgtgac agagcagccg ctcgccgata tcggtcgcta agcagacgtg
     3001 ccgtgcggag ggggtgcttg tcgcggcggt cgatctgtga agaaagaacg gctcctgaag
     3061 cggcttctcg ctactgtacc ggggcggggg taccggaggg aggttgtccg gtcgtggaat
     3121 gaaagtgaaa tcggctcccg ccttgctgct tggcggcata catggcctta tcggcatgtc
     3181 tgagaagttc gtcgatatcc tcatcgtcta acgggtagag cgtgatgccg atgctcacgc
     3241 cgatcgagat ccggtgcggt ccgatctcga acggggtgct gatcgactcc acgattcttt
     3301 tggccaccac gagtacatcg gcgacgccgg aaataccttc caggatggcg gtgaactcat
     3361 cgccgcccat gcgcgcgacg gtatccactt cacgaacgca ctccagcagt cgggtcgcca
     3421 cgagtttcag caactgatcg ccgacatcgt gccccagcgt atcattgacg gctttgaatc
     3481 gatccaggtc gagcagcatg atcgccaggg ggtgatcttt ccgtttgctg cgcgccatgg
     3541 catggattaa gcggtcgcgg aagagggcgc ggttgatcag gccggtaagc tgatcgtgct
     3601 gggcaagata ggtcaggcgt tcctgtgccc gcttgcgttc gatggcatag agaatcgagc
     3661 gtgcaagaaa gtcggttccg ccttctccct tcaccagata atcctgggcc ccgtgttgca
     3721 gcgctttgag ggccagcggg tgatcgtcgt gcccgctcag caccaccacc ggcacgcctg
     3781 gactggtcgc taagacctgg gtcacggtac tcaagccatg ggtgtcggga agggagagat
     3841 cgaggagtac cacatcaaac cggtcccggc tcaatctggt caagccttcc gccagaatgg
     3901 gggcatgggt gatctcgaac tggtcgagcg gccattcggc caggaggtct cgggtgagct
     3961 gggcatcgac gggattgtct tcgaccaaca gcagtttgat catcggtgcg tgctcgcgtc
     4021 cgtaagacaa tctggcgagg cgatgtgctg cgcacaagcg gatggctgac agatcatcgt
     4081 gatgctgcgc gccggaacta tcgagtgtgg aattttcatc tcaaatggtg gtgacggccg
     4141 aggggcgtat tcagtgcggg aatctccctg tggctgcagg tagtgcgtga ttgagggacc
     4201 ggcccgaaat cgattctagc acgtcactcc gaagcttcaa acaagagcgc acttcttcac
     4261 gatgatcgtg cgtgatctgc cggcatgccc tgcgtcctgc tgtcgaccga caatgcctca
     4321 tgtgaagaaa cctccttggt tcgggtctac agatgcatgt cctgcccgca aacctgcgat
     4381 ggcgcgtcat gttggtaacg aacagcaacg aaacaaagaa caaaatccgg ccaaatggtg
     4441 gcacaaaacg gttcggttcc tctccccgct caccagggga gagactcctt gtgaacgccc
     4501 taaccgtacg aagttattgg agctgcctgc ttttgtacac gtcaggacga tggcatgaaa
     4561 gctgcgcttt agcctggggt tcgcgacgct gtggattccc cggctatggg ttgctgcggg
     4621 aacaagagga ggaagagatg gatcacgaaa aagctgtccg gcaggagttg cgagtagtga
     4681 gccataacct tggcggccag agtgacgatg gcgggcgtgt gactgccatg tgggcgcttc
     4741 ctctggcgct tgcgggggtg ctggtggccg gggagcccgc ctctgcagac agcgagattt
     4801 atggctatgt ggggccgaac ggggtgttcg aaatgacgaa tgttccgacc gaccaacggt
     4861 ttcgctcggc ggaatcgcag gtgcgccgcc tgtcgcatcg cgcctcggcg gaggaagtgg
     4921 aggaagccgt caagcgctac gccttccagt ttcaacttca tccggcctta ctgctggcgg
     4981 tgatcaaggc ggagtcggac tttaatccca ccgtcatttc acgatccggg gcggttggac
     5041 tgatgcagct gatacccgaa accgccatca ggcacggggt gctcaatctc tatgacaccg
     5101 acgacaacat tcggggcggc gcgcggcacc tgcggtactt actggatcga tttaacggga
     5161 acgtgcggtt ggcggtcgct gcgtataatg caggtgaacg gcgcgtggag cggtatcgcg
     5221 caattccgcc ctatccggaa actcgcgact atgtcaggaa ggtgatgatc tattacaaga
     5281 gcttccgtgg tgactaccag gcgagccccg ggaaggccgt gttgctcgct atgtatcaca
     5341 agccgctgca acttgagcca ctgcctgtct ctactgatgt gcgaaactca cgacctgcgg
     5401 tcaggaaata gggggggccg agtatctcat cttctctaac gttcccagac gaggcggaca
     5461 aataaccagg ccgaccattc ggtcggccga tacatcacat catcaagata gggttacgag
     5521 gcgtagcgca catttagcag accgaatgcg tggcgttggt caacagccac cagtgctggg
     5581 tggccacgac cagcgtggct ctcttgcctg ttcaatcttc aacactcccg gccttctttg
     5641 ccgtgcaagc ttgggtggtt tcatcagggt aatagggcgc ccttcattcg gcgaatgctg
     5701 cgcgctcggt cacgctcaat cgtaacaatg tgcgaccgca ccatcgcaca agtccgtcat
     5761 cacatgggcc atgaagtaat gaactggttc ggctatgatg cgtcacatgt ggtgacggtt
     5821 ttgatagggc aaacgagggg caagggagag ctttactgaa gtcgcacatc gccgaaataa
     5881 acgacggttt gtgctcgata gcaatgcaaa tcgcggcgga cgagactgaa taggggcacg
     5941 gtgatctagc catagaactt cgcgacatga ttgatgccgt gaaggcgaga attgtgcggc
     6001 ggggtcacgt tattaccgac tgacactgta atttccagcc gccgcggctc atccgatcct
     6061 cagtatgtcc ttgaataggg gccttactat gatctcagta ttcggttcat gcgtaggaca
     6121 agaggaaatt gacgagatac gcaccagctt tgaaaagcaa tggatcggaa tcggtctgaa
     6181 aacaagatca tttgaatctc tcttcgctca caggttgggg ctcaaggatt tcgtgtttct
     6241 ggacaacggt tccaatagtt tatacatggc tgtaaaacta ttaaacctat cccctggatc
     6301 ggaggtcata ctgccctctt ttacatgggt cgcatgcgcc cacgctatca tcctcgctgg
     6361 aggtattccc gtattttgcg atgtagacgt gaatactcaa aacgtgactg ctgacacaat
     6421 tgccccgcat atttcctcaa agaccagggc tattatggtt gtacattatg caggcaaacc
     6481 ggtccgtatg gagccagtgt tatcgtttgg acaaccagtg atagaagatg cggctcaagc
     6541 cgtagattcc aaactaggca ataaatattg cggctctatt ggaacaatag gcgtctatag
     6601 ttttgatcct gtcaaaaata taacaacagg tgagggagga ggaattacaa ccagagattc
     6661 cgaattaatg actcgggccc gacagttgcg gtattgcggt attggaagcg caggatttga
     6721 ggctgcggca atgaaagagc gctggtggga atatgacatc gtagatgttt ttcctaaact
     6781 tcttcccaat gacattgcgg cttcaatcgg actggcgcag ctacgcaaga tagataaatt
     6841 tcaaacgatc cgtaagaaaa tttggaatat atatcaagct caattagctg atttcgactg
     6901 gctgataaga cctcaagacc ctgcttcaga tgaacagcat tcctatttca cttactccat
     6961 tcgggttgcc aaaggaaagc gcgatcaatt ggctaaatac ctctattccc gcggtatcta
     7021 tacaactgtt cgattccatc cattgcatct gaatcctatc tataaatcat cttacaggct
     7081 tcccatttgt gagcagctta acgaggaaat acttagtctt ccacttcacc caaatctttc
     7141 tgatagcgac attgatgcga ttttattaag tttgcaaaaa tttaatgaac agtacatgtg
     7201 agtacagcgt ccgttgtgat cactgtatcc acggaataat tgcgttcttc aaaggcactg
     7261 gtaggtaatc gtggttattg ctgaaggtca catttgcgat aaaagatgtg tcggcgcata
     7321 agcagctgtg tgacccaaag aggccgtcag ccgagaggat cgtgcgccgt attcgcaggc
     7381 cattcccgga catgttgagg gcagtgagca gaaggcagtt attcttgtga caaagcactc
     7441 cacgggacaa ttacaacgtt cgcggggatc agaatagttc ttccctgatc tgcgcatcgt
     7501 gcgttgcaga gcattgttca gggaaagccc gcatgccacg tgtatccgtc atcatccccg
     7561 cctataattc tatgcagtgg gtctctgctg ctatcgacag cgtcctgagc cagggttatc
     7621 gggactacga agtggttgtg attgatgatg ggtcgaccga taagacggca gacgttcttg
     7681 agtcctacgg cagaaaaata cgatgtattt atcaagccaa tgctggtgta tccaacgcta
     7741 gaaaccgcgg cctgagcgta tcgaccggcg aatttatcgc gtatctggac gccgatgata
     7801 tgtggtattc gcagaagctc gaatgtcaaa tcgcgttctt ggatgcccat ccccaatgcg
     7861 gccttgtgca cagcgatgta accgttattg atgaacagga gaaggtcatc cgtgcgcgct
     7921 ttaatgtgga aaccgctagg cccgttccct caggctattg tctgcaagac cttctgcgac
     7981 gctgtcacat ccaggttcca tcggtcattg aacgacggga gtgtatcgaa gaggttggcg
     8041 ggtttgatga gcaatttatg gcaacagaag attatttgca ttggattatg gtgtcactga
     8101 agggatggga atttggatac atcaatgagc cgttagcgaa gtatcgttgg cgaggcggaa
     8161 gcgtttcgtc caacaagcga ctcttcctgg aggagtatgg gaagatgttt ggtgtactac
     8221 tgcccgcttt atacgagaat cgtccccaag caccggattt ggccatcata cgcaagagat
     8281 ttctgcaggt tgaacgcgaa ttggcatata tggattgcat agaagggcat ctgagtagcg
     8341 cgaggcagcg acttatgaga ctgatacagc gatcaccgtt ccgatataac ctctattggg
     8401 acctggcaaa gacctacttg cgctagtacg accgaaattc aaagctactt ctatttggat
     8461 cacattcggg cacacactga agccgctgga ggcatatcgg ggcagtcctt gctggtaagc
     8521 gggcaagcca ttccaggtaa acctcgcacc ccttcctgct ggttcaacat gcttcctggc
     8581 tgatgtcagc gctcaaagtc ccttcaggct gacgtcgagt tccctgcgag tcgtcgtacg
     8641 gcgtgagccg ctccactgcc acctgccgaa aggtactgca aattcccccg cgcgtttctt
     8701 cgccacacgt ctggtgctaa gtgcgccaat ccgttgcggc tcgctctagg gtggcagtcc
     8761 atggctgctc gtgcgagtgc tgaaactgct gtatgtggtt gaaagagaag agagaaggtt
     8821 ggttgcgggg gccggatttg aaccgacgac ctttgggtta tgagatgctt atggggtgat
     8881 ttgctaatgc tctgatcttt cgtctggttt ctcgctttat ttaattgaat cagtgccttg
     8941 taagattcta tatggtccag ttcttttgag tgattctgtt ctgtttcggc gaggtttagc
     9001 acaaatttag cacacagcga tggggtgaac ccctcgggtg agacagtgtg gattagcaga
     9061 cttggtggct atccaaaaat cctcgcgtgg cctgagccga gatcgactga tgaaggttgg
     9121 ttcgaaatcg ccggatggca tatgcagccc agcactctga gacaagatca ggcccgactt
     9181 tgtctggcgt ttcgaggtcg gcagcacaaa ggagcaggat tcaactcctt tgtcggatct
     9241 gacagggctc acaaatggta tcaaaccacc acaggtcttc aggactgacc ttgtaccttc
     9301 ccatggcgac cgcttcctcc tgccaagttt ccttggaggc ttcccccgtc tccgcaactt
     9361 tcactcgttc gcacctcttg cagtggaacc acaccgggcg accaaagagg gcgactaaaa
     9421 actcgttccg gttcattggt cacatttcat tggcctacgg catttattgg ccctcctgtg
     9481 agggctcctc cgccagaaga tcccttgtaa ggcacctcgc cagaatgggg aactcgtaaa
     9541 gcccgatagt tttggatgga agattacgag gtatgaagat cagcagaaac tgcgggcaac
     9601 ctatgtgaga tctcgggaag gagttaggaa gaggccagaa ccccatgcgt cagcgcatat
     9661 ttgatcaaat ccgccgtggt gcgcagatta agctgctcca tgacctgcgc cttgtgaaat
     9721 tctaccgtcc tcgtcgaaat tttcagccga agggcaatgt ccttcatggt ccggccttcg
     9781 gccaacagct gcaagacttc acgttgacga aaggtaagcg tttttttctg ttgcggttgt
     9841 cttgatccag tggtgagcaa cacgtcgagc aggtctttcg caattaaggg cgtgatgtat
     9901 gtatgcccgg cccatacagc ccgaatcgcc tgctccaatt catctaccgc ggaccgcttc
     9961 agcagatacc ccgaggcccc cgattcgaac gcggatcgca cgtaatcggc atcggaatgc
    10021 atggtcacaa agatgacctt gaccgacggc tgaacttttt tcagctgcgt ggctgcatcg
    10081 ataccgttca acagcggcat ggatacatcg agaatgacaa tgtcgggctc cagcttccgt
    10141 gccgcctcaa ggagcgcccg tccatcctcc accataccca cgagctcgca ttggtcttcg
    10201 agaatgcggc gaaagccctc cagcactaac gaatggtcgt cggccaacaa cacgcgcggc
    10261 ttagtcatga ttgctccctg ataacggcac gtgtacttcg acatgtgtcc cgcagccctg
    10321 cgctgcaatc acatccaaag ttccccgaac cagtcgcacc cgttctttca tactcaagag
    10381 accgagacgc ccacgctcct gtgacgctcg cgatagatcg aagccgatcc cgttgtctcg
    10441 tatcgagagt gtgatcatcc cctcgtcgca gatcaattct acttccacct cagaggcgtg
    10501 cgcatgacgt gccacgttgg tcaagctttc ctgcgcaatc cggtacacgc acgtcgcaag
    10561 gtcggtcggc acggggctca cgggatcccg gtgcacataa accgcttcca gtcccgtgct
    10621 ggcggaaaaa tcatcgac
//
LOCUS       DAORQN010000042        10541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_350_length_10541_cov_20.457372, whole genome shotgun sequence.
ACCESSION   DAORQN010000042 DAORQN010000000
VERSION     DAORQN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_350_length_10541_cov_20.457372"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK614_04290"
     CDS             complement(<1..341)
                     /locus_tag="PK614_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81459.1"
                     /translation="MRYFCTYFDRNYLSRGLALYRSLVRTSSSFQLWILCMDSESYDL
                     LTRFRLPQVRLISIEELEAADPELSATKGTRSRIEYYFTCSPSLPLHILKSNAEIDVI
                     TYVDADLYFFS"
     gene            complement(341..1435)
                     /locus_tag="PK614_04295"
     CDS             complement(341..1435)
                     /locus_tag="PK614_04295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81460.1"
                     /translation="MARKPEIDQAIQRVLSGGRYILGPEVAAFEQEFASFLGAGFAVG
                     VGSGTEALHAALRVCGVGPGDTVITVSHTAVATVAAIELAGAIPLLVDIDPKSYTLDA
                     NQLEDTLLAGRSLRIKALIPVHLYGHPADMPSIMSIAERFNVKVIEDCAQSHGASIGG
                     RKVGTWGHLAAFSFYPTKNLGALGDGGAVVTNDLTLAEHTALFREYGWKQRYISEIPG
                     ANSRLDELQAAILRVKLRFLAQDNAKRQAVAGQYDARLSGSSLTIPRTYPGHTHVYHQ
                     YVIRTRHRDELKEFLEKSSVGSLIHYPVPIHLQPAYRDCRLAQGGLTETERICCEILS
                     LPIHPYLTADEVGRVADAILSWLQQEPQPK"
     gene            complement(1495..2484)
                     /locus_tag="PK614_04300"
     CDS             complement(1495..2484)
                     /locus_tag="PK614_04300"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRB81461.1"
                     /translation="MTGSGNFSGSNVLITGGMGFIGSNLARELVGRGAHVTLVDSLVP
                     EYGGNLFNVADIEGNIRVNISDVRDEYSFRHLIQGQDFLFNLAGQTSHLDSMENPYTD
                     LEINCRAQMSILEACRRYRPNIKIVFASTRQIYGKPDYLPVDEKHLLRPVDVNGINKM
                     TGEWYHILYNNVYDIRSCALRLTNTYGPRMRIKDARQTFLGIWIRLLAEGRPFDVWEG
                     HQLRDFTFVSDVVDAMLLAAVSDDANGQVFNLGGEPAISLKDLADLLVKVNAGGSYVL
                     RSFPGDRKRIDIGDYYANFEKIRTTLEWEPRVSLEQGLHRTVEFYRLHLAHYL"
     gene            complement(2481..3209)
                     /locus_tag="PK614_04305"
     CDS             complement(2481..3209)
                     /locus_tag="PK614_04305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB81462.1"
                     /translation="MLTRLKRFYTAQMMCPNFAGLFINPFYFARKGLYDGIRGLAPRI
                     TGRVLDVGCGTQPYRRLFSTAEYTGMELDTQENRAHKRADVFYGGQIFPFDSESFDSL
                     VCSQVLEHVFTPREFVGELNRVLKPGGALLLTVPFVWDEHEQPYDCARYTSFGLLALM
                     KEAGFEVVEHVKTMGDVRVLCQLLNAYLYKVTVSKSPYLNLLSTLLLMAPINLFGAVA
                     RFVTPSNLDLYLDNVILARKAGHE"
     gene            complement(3203..4024)
                     /locus_tag="PK614_04310"
     CDS             complement(3203..4024)
                     /locus_tag="PK614_04310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, TIGR04325 family"
                     /protein_id="HRB81463.1"
                     /translation="MKRVIKSWIPPAILNAYRSFCSDDIRFHGQFSTWESARAASSGY
                     DDEVIFRKSRAAALKVKKGEVAYERDSVLFDHVEFSYPILSGLLRSARGRDGNVSVLD
                     IGGSFGTTYRQFKAFLRPSASVRWNIIEQPRVVESGQAEFADEELHFFRTIGEAADAE
                     FPDVVLLSSVLQYVQNPYDLIREVCSIGARSIVVARTPCALAERDILTVQVVPPSIYK
                     ASYPCWIFAESKLLEAFSARYRLVASFQEPGGSLWSDQGPFELRGFILDRKSSRC"
     gene            complement(4021..4938)
                     /locus_tag="PK614_04315"
     CDS             complement(4021..4938)
                     /locus_tag="PK614_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81464.1"
                     /translation="MLAPIGLFVYKRPEHTVRVLEGLRSNREARDSDLFVFSDGAKNS
                     LANEGVSAVRALVRGLTGFRSVTVVEQSANLGLARSIVTGVTDLTGRFGRVIVLEDDV
                     VPGRHFLHYMNAALEKYEHVREVVSIHGYSYPVNCSLPDTFFLRGADCWGWATWARGW
                     RVFEPDGAKLLARLSESGLSYVFDFDGGYPYTKMLEEQVAGRNDSWAIRWYAAAFLDG
                     LLTLYPGSSQVQNIGTDGSGTHGGCAAEFLHASWGKPVLLEDIPIQENQRAREAFVHM
                     LKSLRPSLTRRILARLRKITGEIPKGVKR"
     gene            complement(4932..5645)
                     /locus_tag="PK614_04320"
     CDS             complement(4932..5645)
                     /locus_tag="PK614_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRB81465.1"
                     /translation="MHGFLSRCLTSCLYSMLRAIRPEVRWSYSQEGEDLVLSRLFENQ
                     RHGFYVDIGAHHPFRFSNTYLLSRRGWRGINVDPNPQAMRLFGRLRRRDINLAIGIAE
                     EAGSRRYFQFNESALNTFDEQLARQREVKDGYQLIASTLVSVQPLRQVFEQHLPQGQA
                     IDVLSVDAEGCDLSVIRSNDWDRFRPYCLLVETLQSSLTSLETNATHQYVSARGYGLF
                     AKTVNTFIYLDQKSPRQPC"
     gene            complement(5673..7439)
                     /locus_tag="PK614_04325"
     CDS             complement(5673..7439)
                     /locus_tag="PK614_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81466.1"
                     /translation="MAGITSPFGIDRTAIVSWLTTIHSLVAYAARKFPLLAACVLLSL
                     ISVAMELTAMASLVPLMELAVGHVIPSTSKWSSVPRWLGYTPDIAFYVMMFLLLISLR
                     LITSFASSLLVSYLSRQMIAHFSSEAFTAFVNSLAFEEIQQRSVGYFINLAGDEANRA
                     SQVITALLRLIPVAVLGLLYFSAVTYQSWWIAIGVLAFLASSLAALGQTFRRVHELGA
                     RQQQESRDLNSHFLDSLNSLRTVRSYTAEDFVSQEYQRMIYGYAGTCFRVDALSLLSS
                     FVPALILVLASGAAILWFIDVSAIAAHFSVIMVAVVLLLRFFPVAGQAIDIFLRLVAD
                     LRAGDDLRRVLRDVSAGQVTEPAQDECAVAPPIHHIRFETVSFAYAQREPVLQAFTVS
                     FCAGNNYAVLGRSGIGKSTFVDVLLKFYVSHSGRITVNDVDLSRVNTQWLRDRIRVVE
                     QHAKLFNDTIYNNVTFGAQVTLDMVREACRIACIDSDIERLAEGYNTLVTYQGSNLSG
                     GQRQRLAIARALVRNPDVLILDESVNALDAVLRRQVVTSILQAYRQRIVICITHDPAV
                     SEQFERVLEFTRLKAEPQPLSS"
     gene            complement(7427..8455)
                     /locus_tag="PK614_04330"
     CDS             complement(7427..8455)
                     /locus_tag="PK614_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRB81467.1"
                     /translation="MTQSKGQTALQPVLEKLLGHQRLIIADAGAAYGLPAHLQMLASV
                     SLLCLFEPHAQRAEELRRLLGAEARVFQTALSRQGGERTLYVTNVPTGASLLRPGSDA
                     AFELAEHAYFFPVQEERIHTRRLEDVLVEDGIARLDFLKADVQGAELEILQGLGASLG
                     RTLLGVELEVGFPGGYIGQPGFGDLDQYLRDLGFELLDLRLARIHRFVSGSYRHLSEH
                     VFGVHADSSSLSKRVWEADVLYFVKPATLMHAGDVPGIRRLAVLYCAYGFYTEAYHLL
                     EQAGQVGMLSSEEAAELKGDVVRLHRMVRSCVFESVMWHRLTSPFRRFGIRLRNTLCG
                     FQGGKWQE"
     gene            complement(8462..9661)
                     /locus_tag="PK614_04335"
     CDS             complement(8462..9661)
                     /locus_tag="PK614_04335"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRB81468.1"
                     /translation="MTQDVAGVDAAFHDLLRQADRATGGEEARFNEAVGCRIDALQDY
                     VVGAGGLTGRIAEYSIRAIRMARQSLLDEWEFQVRAHHLRYQTVLSAAQHTKLETLRK
                     DGVICLEEKRELASRLWDKMWWERGLLRQQAARLPWGRCAMALDPHSPAGLMVQAALK
                     HSGVFELAGAYLGCRMEFLYAALDHAHERQAWYKGCYADVGLGTSETAYMHLDADCDI
                     VKAMLYLTDVNSENGPFRFVRGSHQWARSPFLIALYKGFDCEQDVYFERAQNTLDYEG
                     GYYRPRFQAAAHRRELMCLPSSFRGSTHFGDDVVDASPLSKRLLAHEDVFTGCGGTFV
                     LFHGAAGIHRGAQVSAGQRWAIQIALRASREQSERPHRYVKERLASLRYHIRRLKRTL
                     LLTSKAT"
     gene            complement(9825..>10541)
                     /locus_tag="PK614_04340"
     CDS             complement(9825..>10541)
                     /locus_tag="PK614_04340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRB81469.1"
                     /translation="KSAQERRSDYFDSGAGTTLQKLHSFMRFVSRQDVAKHRAYVSLF
                     ETTRNVVGSIAECGIYFGQGLMTYANLAAAFEPYNYQCRILGFDTFEGDRSHSESDEK
                     GHVVDRNVWNYEAKVLEDLRQAIAIYDLDRPLNHLEKVKLIQGDLCETAPAFMAAHPE
                     TLFRIIHLSVNLYLPTRSVLQHFYPKLSRGGILAIHGLNYSAPGAQKALTEVVDQEFG
                     ERLNLCTFEYYPNLVYSVRP"
BASE COUNT         2724 a         3069 c         2553 g         2195 t
ORIGIN      
        1 tcggagaaga agtacaaatc cgcatctaca taggtaatga cgtcgatctc agcattggac
       61 ttgaggatgt gcaatggaag agacggagaa caggtaaaat agtactcaat cctggaacgt
      121 gttcctttcg tagccgacaa ctcaggatcg gcggcttcga gttcttcgat tgaaatgagc
      181 cgaacttgcg gcaatcggaa tcgagtcagc aaatcataac tctcactgtc catgcaaaga
      241 atccacagtt gaaacgaaga agaggtccgg acaagcgagc gatacaacgc caacccgcga
      301 gagagataat tgcggtcaaa atatgtacaa aaatagcgca tcacttaggc tgaggctcct
      361 gctgaagcca cgacagaatc gcatcggcga cccgaccaac ctcatcagca gtcaagtatg
      421 gatggatcgg aagactaagg atctcacaac agatccgctc cgtctcagta agccccccct
      481 gtgcgagccg gcagtcgcga taggccggtt ggagatggat cgggacagga taatggatca
      541 gcgatcccac agaagatttt tctaaaaact ccttcaattc gtcacggtga cgagtacgga
      601 ttacgtactg gtgatagacg tgcgtgtggc cagggtacgt tcgcggtata gtaagcgacg
      661 agccagacaa tctcgcatcg tactgaccgg ccaccgcctg ccgcttcgca ttgtcctgag
      721 ctagaaaccg aagtttcact cgcaggatag cggcctgaag ttcatcaagc ctggaattgg
      781 caccgggtat ctcgctgatg tagcgctgct tccaaccata ttcgcgaaac aatgctgtat
      841 gctcagccag tgtcagatca ttggtcacca ccgccccgcc atcccccaaa gctcccagat
      901 tcttcgtagg gtaaaaactg aatgccgcca aatgccccca cgtaccgact ttccggccac
      961 cgatcgaagc tccgtgggac tgagcacaat cctcaatcac cttcacattg aatcgctctg
     1021 cgatgctcat gatcgagggc atatccgcag gatgaccgta aagatgaaca ggaatgagcg
     1081 ctttgatacg caacgatcgg cccgccaaga gcgtatcttc cagttgattg gcatcgaggg
     1141 tatagctctt ggggtcgata tccacgagga ggggaatcgc gccggccaat tcgattgcag
     1201 ccacagtagc cacagcggta tgagagacgg taatcaccgt gtcgcctggg cctacaccgc
     1261 aaacgcgcag agccgcatgc aacgcctctg tgccgctgcc cacacccaca gcaaaaccag
     1321 cccctagaaa ggaggcaaac tcttgctcga atgcggcaac ctccggccca agaatatacc
     1381 ggccccctga cagaacacgc tggatcgcct ggtcaatctc tggcttccga gccaagtagt
     1441 ctgcgagtgg gttagcggct gagatcacag gcaaatgcga cacgacgcgc tttcttaaag
     1501 atagtgggca agatggagcc gataaaactc gaccgtgcga tgaagccctt gctctaacga
     1561 cactctaggt tcccattcga gtgtggttcg aattttctcg aaattcgcat aatagtcacc
     1621 gatatcaatt cgcttccggt ctcctggaaa cgaccgtagg acataactcc ctccagcgtt
     1681 gaccttcaca agtaaatcag caagatcctt gagactgatt gccggctctc caccaagatt
     1741 gaatacctga ccattcgcgt catcactcac cgcagccaag agcatcgcat caacaacgtc
     1801 gctgacaaat gtgaaatctc gtagttgatg tccttcccac acatcaaacg gcctcccctc
     1861 ggcaaggaga cgaatccata tccccaaaaa cgtttgcctg gcgtccttga ttcgcatccg
     1921 aggcccgtag gtattggtca ggcgcaatgc acaggaacga atgtcgtaca cgttgttgta
     1981 caggatgtga taccactccc cggtcatttt attgatccca ttcacgtcca ccggccgaag
     2041 aagatgcttc tcatccaccg gtagatagtc cggcttacca tagatctgtc tcgtactcgc
     2101 aaaaacgatc ttgatattcg gtcggtaacg acggcacgcc tctaaaatcg acatctgggc
     2161 gcggcaattg atctccagat ccgtataggg attttccatt gagtcgaggt gacttgtttg
     2221 ccctgccaaa ttgaacaaaa aatcttgtcc ttgaatgaga tgcctgaaac tgtattcgtc
     2281 acgaacatct gaaatattca cgcgaatatt tccttctata tccgcaacat tgaatagatt
     2341 gccgccatac tctggaacga ggctatcgac cagcgtcaca tgcgcccctc ttcccaccag
     2401 ctctcgagcc agattggagc cgataaatcc catccctccg gtaatcagga cattcgagcc
     2461 tgaaaagttt ccagatcctg tcattcgtgc cctgcttttc tcgctagaat aacgttatcg
     2521 agatacaaat caagatttga aggagtgacg aatcgtgcga cggcgccaaa gaggttgatg
     2581 ggtgccatca acagcagggt actcagaaga ttgaggtatg ggctcttact cactgtcact
     2641 ttatacagat acgcgtttaa caattgacag agcacgcgaa catctcccat tgtcttgacg
     2701 tgttcgacaa cttcaaagcc ggcctccttc atcagagcaa gcaggccaaa cgacgtgtac
     2761 cgtgcgcaat cgtacggctg ttcatgctcg tcccacacga atggcaccgt cagcagcaag
     2821 gctcccccag gcttcagcac tcgattcagt tctccaacaa actcccgtgg tgtgaagaca
     2881 tgctccaaca cctgactaca cacgagggaa tcaaaacttt cagaatcgaa cggaaagatt
     2941 tggccgccgt aaaacacatc tgcccgcttg tgcgcgcgat tctcctgcgt atccagctcc
     3001 attcccgtat attcagcggt cgaaaataat cgccgatacg gctgcgttcc acagccgaca
     3061 tccagaacac gccccgtgat tctgggcgcc aggccgcgga tgccatcata cagccctttt
     3121 cttgcaaaat agaacgggtt gatgaacaag cccgcaaaat ttggacacat catctgggca
     3181 gtataaaacc gcttgagcct ggtcaacatc tggaagactt tcggtccaag atgaaaccac
     3241 gaagttcaaa cgggccctga tcactccata acgatccacc aggctcttga aatgaagcca
     3301 ccagtcgata ccgcgccgaa aacgcctcga gcaatttaga ttcagcgaag atccaacacg
     3361 gataacttgc cttataaatc gaaggaggca caacttgaac cgtgaggata tcccgctcgg
     3421 caagggcgca cggcgttcgt gcaaccacaa tactgcgcgc gccgatggag caaacctcac
     3481 ggatgagatc gtagggattc tggacatact gcagcacgct cgacagcaat acgacgtcag
     3541 ggaattccgc atcggcagcc tctccgatcg tgcggaagaa atggagttct tcatccgcaa
     3601 actcagcctg ccctgattcg acaactcgag gctgttctat gatattccac cgtacggatg
     3661 ccgaaggcct gagaaaagcc ttgaactgtc gataggtggt tccaaatgat ccgccgatat
     3721 ccaatacgct cacattcccg tctcgtcccc gcgcgctacg caagagaccc gacaggatag
     3781 gataagaaaa ctccacgtga tcaaatagaa cagagtcgcg ttcatacgcg acctctcctt
     3841 tcttaacctt gagcgccgct gctctggatt tcctgaaaat gacttcatcg tcatatccgg
     3901 aagaggccgc ccgcgctgac tcccaggtag aaaactgacc gtgaaaccga atgtcatcag
     3961 agcaaaagct acggtaggca ttcagaattg cgggagggat ccaggacttg atcacgcgtt
     4021 tcacctcttg actcccttcg gaatctcgcc ggtgattttg cgtagacgcg ccaaaatcct
     4081 ccgagtcagt gacggccgta gcgatttcag catatgcaca aatgcttcac gtgccctctg
     4141 attttcctgg atcgggatat cctccagtaa gaccggcttt ccccatgagg catgcaagaa
     4201 ctcagcagca catcccccat gagtcccgct cccgtcggtc ccgatgttct gcacttgaga
     4261 cgagcctgga tacagcgtga gtaacccgtc caggaatgcc gctgcatacc atcggatagc
     4321 ccaggagtcg ttccgccccg ccacctgctc ctcgagcatt ttcgtgtagg gatacccgcc
     4381 gtcgaagtcg aacacgtacg acagcccgct ttcggaaagc cttgccaaca gcttggctcc
     4441 atccggctca aaaacgcgcc agcctcgcgc ccacgtggcc cacccccaac agtctgcgcc
     4501 gcgcaggaag aacgtgtccg gcagtgagca gttcacagga taggaataac cgtgaatgct
     4561 gacgacttct cggacgtgct catacttttc taacgcggca ttcatgtagt gcagaaaatg
     4621 ccgccccgga acaacgtcat cctccaatac aatcacccgt ccaaatcgtc ccgtcaaatc
     4681 cgtgactccg gtcacgatcg aacgagcaag cccgaggttt gcagattgtt ccacgacggt
     4741 gacggaacga aatcctgtca gtccacgaac caacgccctg acggcagaaa ccccttcgtt
     4801 cgcgagcgag ttcttcgctc catctgagaa gacaaagaga tccgaatccc gagcttctcg
     4861 gttgcttctc agaccttcca gtactcgcac agtgtgctca ggacgcttat acacgaacaa
     4921 gccaatcggc gctaacacgg ctgtctcggg ctcttctggt ccagataaat gaaggtattg
     4981 accgtcttgg cgaataggcc gtaaccgcgc gccgatacat attgatgcgt cgcgttcgtc
     5041 tcaagggagg tcaacgagga ctgcaacgtc tccaccaaca agcagtacgg ccgaaaccgg
     5101 tcccagtcat tcgaccggat gacgctcaga tcacatcctt ccgcatccac cgacaagacg
     5161 tctatggctt gtccttgcgg caagtgctgt tcaaatactt gacggagcgg ctgtacggaa
     5221 accagtgtcg acgcaatcaa ttgatagcca tctttcacct cgcgctgacg ggccaactgt
     5281 tcgtcgaacg tgttcaacgc agactcattg aattggaaat accgccggct tccggcctcc
     5341 tcggcgatac ctatggctag attgatatcg cgtcgacgca gacgtccgaa caaccgcatc
     5401 gcctgcggat ttggatcaac attgattccc cgccaaccac gacgagagag caaatacgta
     5461 tttgaaaatc ggaatgggtg gtgtgcgcca atgtccacat aaaagccatg acgctggttt
     5521 tcgaatagcc gactcaaaac gagatcttca ccctcttggg aataggacca acgaacttca
     5581 ggacggatcg ccctcagcat ggaatacagg caggaggtca gacaacgtga gagaaaacca
     5641 tgcatggcgt ccaatggacc gatcgatctc gcttaggaac tgagtggctg cggctccgcc
     5701 ttgagccgtg taaactccag cactcgctcg aactgctccg acactgcagg atcatgagta
     5761 atacaaatca cgatccgctg tcgatacgct tgaagaatac tcgtcaccac ttgacgacgt
     5821 aataccgcat ctagtgcgtt gacgctttca tccaaaatca gaacatcagg attccgaacc
     5881 aatgcccggg cgattgccag tcgctgtcgt tgccctcccg agaggttgct tccttggtag
     5941 gtaaccaaag tattgtaccc ctccgcaagg cgttcgatgt cggagtctat gcaggcgatc
     6001 cgacaagctt ctcgaaccat atccaacgtg acctgcgcgc caaaggtgac gttattatat
     6061 atggtgtcgt taaaaagttt ggcatgctgc tcaacgaccc ggatacgatc ccgtaaccac
     6121 tgtgtattga ctcggctgag gtcgacatca ttgaccgtga tgcgcccgct gtgagacacg
     6181 taaaacttaa ggagaacatc gacaaacgtt gatttaccga taccagaccg gccgagcact
     6241 gcatagttgt ttcctgcgca gaatgacacc gtgaacgcct gcaacaccgg ttcgcgttgc
     6301 gcatatgcga acgatacggt ttcaaaccgg atatgatgaa ttggtggagc gaccgcgcac
     6361 tcatcttgag caggctccgt cacctgccca gcggacacgt cacgaagaac ccgtctcaaa
     6421 tcgtctcctg cgcgaagatc agccaccaag cgcaagaaaa tatcgatagc ttgacctgca
     6481 accgggaaaa accgcaataa gagaacgact gccaccataa taacggaaaa atgcgcggca
     6541 atagccgaca catcgatgaa ccacagaatc gcggcaccgc ttgccagaac gagaataagc
     6601 gccggcacga agctagacaa cagagaaagc gcatcgactc gaaaacaagt tccagcatac
     6661 ccgtagatca tccgctgata ttcctggctc acgaagtcct cggccgtgta actgcgaacg
     6721 gttcgcaagg aattgagcga gtcaagaaag tgcgaattga gatctcggga ctcttgttgc
     6781 tgacgcgctc ccaactcatg cacccgtcga aacgtctggc ctaaggctgc cagcgacgac
     6841 gccaggaacg ccagcactcc gattgcaatc caccacgact gataggtaac ggctgaaaag
     6901 tacaggagac cgagcaccgc cacgggaatc aagcgtaaca atgctgtgat gacctgactc
     6961 gcccgattcg cctcatcacc ggcgaggttg ataaaatatc ccacgctacg ctgctgaatt
     7021 tcttcaaatg cgagtgaatt cacaaatgcc gtgaacgctt cggatgagaa atgcgcgatc
     7081 atttgccgag agagatacga caccaggaga ctcgacgcga aggaggtgat caaacgcaaa
     7141 ctgatcaaca gcaaaaacat catcacatag aaggcgatat ccggagtata gccaagccac
     7201 cgcggaacac tgctccattt gcttgtcgaa ggaatgacat gccctaccgc caattccatg
     7261 agtggaacca gactcgccat agcagtcagt tccatcgcca ccgagatcag agacaacagg
     7321 acgcaggccg cgagcaacgg gaactttcgc gcagcatacg cgacgaggga atgaatggtc
     7381 gtcagccatg acacgattgc ggtccggtct atgccgaacg gcgacgttat tcctgccatt
     7441 ttccgccttg aaacccgcag agtgtgtttc gcaagcgaat cccgaagcgg cggaatggac
     7501 tcgtaagccg atgccacatc accgactcaa agacacaaga tcgaaccata cggtgtagcc
     7561 ggacgacatc acctttaagt tcggcagctt cttccgaaga aagcattcct acttgccccg
     7621 cttgttcaag caggtgatag gcttcggtat agaacccata cgcgcagtac aacactgcca
     7681 ggcggcgaat tcccggcaca tcgccggcat gcatcaatgt cgccggcttc acaaagtaca
     7741 gcacgtctgc ctcccagaca cgcttactga gtgaagaaga atcggcatga acaccaaaca
     7801 cgtgttctga aagatgacgg tagcttccgg acacgaatct atggattctc gccagccgca
     7861 aatccaatag ttcgaaaccg aggtcacgaa gatattgatc gagatcccca aatccaggct
     7921 gcccgatgta tccgccggga aatccgactt ccaattcaac ccccaaaagt gtgcgcccaa
     7981 gcgatgcgcc gagtccttgg agaatttcca actctgcgcc ttgaacgtcg gctttcagaa
     8041 aatccagccg ggcaatgcca tcttcgacga gcacgtcctc aagccgacgc gtatgaatcc
     8101 gctcctcttg cacaggaaag aaataggcgt gctctgccaa ttcgaacgcc gcatccgaac
     8161 ccggccgcaa gagggaggcc ccggttggaa catttgtcac atacaacgtc cgttcaccgc
     8221 cttgacgtga cagagccgtc tgaaacacgc gcgcctcagc gcctaagagc cgacggagtt
     8281 cctctgcgcg ctgggcatgc ggttcgaaaa gacacagaag ggatacggag gcgagcatct
     8341 gtagatgcgc gggcaatcca tacgcagcac ccgcatccgc aatgatcaag cgctggtgac
     8401 cgagcagttt ttcaagaacc ggctgaagcg ccgtctggcc cttcgattga gtcacagcgg
     8461 atcaggttgc cttagaagtt aacagcaagg tgcgcttaag ccgtctgata tgatatcgaa
     8521 gactcgcaag tctttccttc acataccgat gcggacgttc cgattgttct ctcgatgcgc
     8581 gaagcgcaat ctgtatcgcc caccgctgac cagcggatac ttgcgctccc cgatgaatgc
     8641 cggccgcgcc atggaacagc acgaaagtcc cgccgcaccc agtgaacaca tcctcgtgtg
     8701 cgagtaaccg ttttgacagt ggacttgcat cgactacatc atcgccgaaa tgagtggatc
     8761 ctcgaaagct gctcgggagg cacatcaatt ctcggcgatg agccgctgcc tgaaaacgag
     8821 gccgataata ccctccctca tagtcgagcg tattctgcgc tcgctcaaaa tacacgtctt
     8881 gctcgcagtc aaaccccttg tatagggcaa taagaaacgg agatcgcgcc cattggtgac
     8941 ttccgcgcac aaatcgaaat ggtccgttct cagagttcac atccgtgagg tacagcatgg
     9001 ccttgacaat atcgcagtct gcgtccaagt gcatataggc ggtctcagac gtacccaggc
     9061 ctacatctgc ataacacccc ttgtaccatg cttgacgctc atgcgcatga tccagcgccg
     9121 catagaggaa ttccatacga caccctaaat acgcaccggc aagctcaaac accccggagt
     9181 gcttcaatgc cgcctgaacc atcaatcccg caggagaatg cggatcgagc gccatggcgc
     9241 accgccccca aggcaatcgc gcggcttgtt gtcttaacaa cccacgctcc caccacattt
     9301 tgtcccaaag gcgtgacgcc aactcccgtt tctcttcgag gcatatcacg ccgtccttcc
     9361 tgagggtttc aagtttcgtg tgttgcgccg cactaagcac cgtctgatac ctgagatgat
     9421 gcgctcggac ttgaaattcc cactcatcca ataacgattg tcgcgccatc cttatggctc
     9481 gtatggaata ctccgcgatt cgaccggtca gtccgcccgc tccaaccaca tagtcctgca
     9541 gggcatcaat ccgacacccg accgcctcat tgaatcgagc ctcctctcct ccggtcgcac
     9601 ggtcagcctg tctcaataag tcgtggaacg ccgcatcgac tcccgctaca tcttgcgtca
     9661 ctcgatcatc atcgagacac cagagggagc ctgtcgatac gtacgcagga tgggaggcat
     9721 agtcgacgca tgggaacggt tctatctctg acatgagcgg cgtaaccatt ggatcgtgtg
     9781 tccctctcgc ttcagaccac ggcttgccag tcttctgtag gcctctatgg tctgacactg
     9841 tacacaaggt tgggataata ttcgaacgtg cacagattta gcctttcccc aaactcctga
     9901 tcgacgactt ccgtcagcgc tttctgcgct cccggcgcac tgtaattgag cccatgaata
     9961 gccaatatgc cgccgcggga caatttcgga taaaagtgct gcagtaccga acgggtcggc
    10021 agatacaaat tgaccgacaa gtgaatgata cggaaaagag tctccggatg tgctgccata
    10081 aacgccggcg cagtctcaca caggtctcct tggatcagct tgactttttc cagatggttt
    10141 aatggccgat caaggtcata aatcgcaatc gcttgccgaa gatcttccag caccttcgct
    10201 tcatagttcc acacatttcg atcgacaacg tgccccttct catctgactc agagtgcgat
    10261 cggtctcctt cgaatgtgtc aaacccaaga atgcggcatt gatagttata cggttcgaat
    10321 gctgcggcaa ggttggcata tgtcataagc ccctgcccga agtagatgcc gcactcggct
    10381 atggagccga caacattccg agtcgtctca aataatgaaa cataggcacg atgcttggcg
    10441 acatcctgcc ggcttacaaa ccgcataaac gaatgcaact tttgtaatgt agtccctgcg
    10501 cccgaatcga aatagtcaga acgtctttct tgtgccgact t
//
LOCUS       DAORQN010000043        10458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_353_length_10458_cov_12.527925, whole genome shotgun sequence.
ACCESSION   DAORQN010000043 DAORQN010000000
VERSION     DAORQN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_10458_cov_12.527925"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1195
                     /locus_tag="PK614_04345"
     CDS             23..>1195
                     /locus_tag="PK614_04345"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRB81470.1"
                     /translation="MTYRHRRRKVLSVLAGIPMIAATLLVGGMQPGSQAQAAGVPPAF
                     AQGFSEIVKAVTPAVVNIAVTGGGEGRREGRRQLPPGGPFGGPPPGPGDEPPGMEPPG
                     GPGGPPGPPGGGPHRPEQSAGSGVILDPNGYIVTNNHVVEGATQITVTLSDRREFPAK
                     IIGTDPKTDLAIIKIEAKDLASMKWADYDELQVGDVVLAVGSPFGLSSTVTLGIISAL
                     GRGNVGIADYEDFIQTDAAINPGNSGGALVNMQGKLIGINTAIFSRTGGSEGIGFAIP
                     SSIATDIVESLTKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSH
                     SAGMRRGDVVIAFNGKEVQSVSQLRNLVARMAVGKDADIKILREGKEQSLKVKVAER"
     assembly_gap    1196..1245
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1246..1545
                     /locus_tag="PK614_04350"
     CDS             <1246..1545
                     /locus_tag="PK614_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRB81471.1"
                     /translation="KPPDNVLASLRVQMLDAAMQSQLNIPAKTSGVVVSSVEAGSAAE
                     SAGLQRGDVIQEVNHEVVKSLEDYQKASAKVKKDEMVVLLLSRQGNNLFVAVNPK"
     gene            1626..2570
                     /gene="tatC"
                     /locus_tag="PK614_04355"
     CDS             1626..2570
                     /gene="tatC"
                     /locus_tag="PK614_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRB81472.1"
                     /translation="MADGFKFQEWLQDTVFKPLEDKKMPVMEHLVELQVRLTRAVIIT
                     AIIFVGTFFYADTLVKWIRVPLQNMFVPGSLSWIPTDLPTVPFVFLAPAEALWQNVKV
                     AGLFALVLGTPYILIEFWHFVVPGLHAQERRFVGPFVILSTLAFYLGLLFSFFFVLPF
                     ALNFLISYGVNAGFIPQLSIAQYVGFALWFLLVFGLIFEVPLVITLLAKLGWVDAPLL
                     KRYRKWAFLAAFIFAAILTPTPDPFNQCLMALPMYIFYEVGIVSAGIFNKKKAGAPEE
                     ALVPAVATAGPGLGKPGSSMPSGGGDSEYVGVPTGRRG"
     gene            2651..3607
                     /locus_tag="PK614_04360"
     CDS             2651..3607
                     /locus_tag="PK614_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRB81473.1"
                     /translation="MPRELNVISQPGSSSGGDACTYMWACAICDENETCQKDKEGHSR
                     WLVAKRMERIEYKVLVMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKM
                     VGAEGQKLKISTSGGIIPFQAYNMKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGV
                     EWQDLNFLLIDLPPGTGNESVTTIDLLGGVSGAVIITTPQEVALLDSRKSVTFCKDSE
                     VPIIGIVENMSGLECPHCHKEVNVFRKGGGEASATDMGVPFLGRIPLDPDVVTQSDAG
                     EPFALFNSDSATAQAYHDIANQVEAFCKKSGSLVKLAPRQQH"
     gene            3622..4920
                     /locus_tag="PK614_04365"
     CDS             3622..4920
                     /locus_tag="PK614_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRB81474.1"
                     /translation="MKAPDATTGLTPLHEAQRIVLDAATPLGLEKISILDTLGRVLGE
                     DIIAERHNPPWDNSAMDGFAVQAEDIQQEHTISKPVTLTVIEDVPAGKMPTKSVGRGQ
                     AIRIMTGAPIPKGADTVLKVEDTEHTPDSVRVFKPEPRGSNIRPQGEDVKKGDCIIPK
                     GTTIRSGEAGMLAILAKSFVLVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQEAGGVPILLGIARDTPAALKEKISHGLNADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKLAGARGYG
                     RPLLEAVFQEKFSKRTDRRHFLRGVLWREDGVFKVRTTGDQGSGILTSMVKANCLIDI
                     PVEAERINPGDSVTVQLLSGSAWLEQAAPMASTGHRPSCC"
     gene            4941..5450
                     /gene="mobB"
                     /locus_tag="PK614_04370"
     CDS             4941..5450
                     /gene="mobB"
                     /locus_tag="PK614_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRB81475.1"
                     /translation="MSVPILSFVGRSNSGKTTLIERVIPELVRAGYKVATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVIISKSSLAMFADVSDHMNVEDVRERYLDASYDLILAE
                     GWRSEGYPKIVVVRDQIGEVPVSQDGLLAVVSNKPVETSVPLLDPDDVAGVAALIIRH
                     FPKPQRDDA"
     gene            5443..6147
                     /locus_tag="PK614_04375"
     CDS             5443..6147
                     /locus_tag="PK614_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRB81476.1"
                     /translation="MRKSSVIVLGAIVAGAVALGGVAVPLTNHPKFCASCHTIRPAYE
                     RWLESSHKEVECVACHVRPGVSGWLQDKAWHGTRDVAIYLFGKPTAPHNLQAHVDSGV
                     CLSCHRNILRVSEIATRDLPAPVKDVGLVMSHRKHMDAFGKRGQGEGCTTCHAAVVHE
                     RPIKGYPIVIPRGHVSADSRPWKPEHPEGSVLHKRAQADCFRCHDNKTEYEGRVLSRK
                     CETCHLPEKLTEFLSF"
     gene            6168..6896
                     /locus_tag="PK614_04380"
     CDS             6168..6896
                     /locus_tag="PK614_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81477.1"
                     /translation="MSPSAVEVRNLTKRFGGFTAVDGISFDIGRGEILGLLGPNGAGK
                     TTTFQMMLGLVTPSSGSIRMFGLDLQTNREAILQQVNFSSTYISLPYSLTVEENLGVI
                     ARLYGMSNIAGRIDDMVKKLEMSEWRNKLTRKLSSGQMTRLTLAKALLTEPKVLFLDE
                     PTASLDPDIAHKIRAILLEERQATGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     AKDIVARFGQADLEEVFLKLAREQ"
     gene            6942..7412
                     /locus_tag="PK614_04385"
     CDS             6942..7412
                     /locus_tag="PK614_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81478.1"
                     /translation="MVDAGVSFGVGLLIGGLGLMLGWGLLWLVVGAIGLARETCGWAI
                     FMSSVSSTVIAGLSMAGVLWVVDSARLGSSALHAGLAGVPLALLVAGFCRLEDGRRIG
                     PAFLEGSRLMLHQLLGVHEEGCGHCHEKPVEEPPCHQKPCYEQSCHEKPCEGKP"
     gene            7419..8210
                     /locus_tag="PK614_04390"
     CDS             7419..8210
                     /locus_tag="PK614_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB81479.1"
                     /translation="MAYHRIAALVIRHLYLYRRSLPRVMEIIYWPFLDLVVWGFITVY
                     LATFQGQMPAVVTFLLGALILWDVLFRSQQGITISFLEEIWARNLMNLFASPLTPSEF
                     LAATMVMSLFKVTAVSMVMSVCAWIFYGYNVFIIGLWLMPFIVNLVLTGWIIGVFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPMEVLPSWLRAVASINPAAHVFEGMRGLLA
                     THAAPLSSLSLASGLNLVYLGVVILWFHHTFSVCKDRGSLVRVGE"
     gene            8246..8920
                     /locus_tag="PK614_04395"
     CDS             8246..8920
                     /locus_tag="PK614_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRB81480.1"
                     /translation="MNINSGQVRPALLPVGLESFSFHQTPVLVIENFWSAEERTQFRQ
                     AMNRANWNQLQDLSYVRQDFPNSGNWAKAEIAQPQGQLLLSRLEMPCIQQYIESFPNI
                     TRRHLGFSYYSYGAGDCLLTHDDTDQGRPVAGKPAPRRRLAMVSYFHEEWECDWGGEL
                     MIYSATGEPTNGKPELAITHCIAPKPGSLVMFTVPRFHRVCRVDQTAGDHKRLSIAGW
                     FMTEHA"
     gene            complement(9005..9613)
                     /locus_tag="PK614_04400"
     CDS             complement(9005..9613)
                     /locus_tag="PK614_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRB81481.1"
                     /translation="MEKKSKKKAPQESVATEDRKKTHVGDIVRRLRKSRHLSVRTLAD
                     KCGFSPSFISQVELRQASPSIASTERIASALGVTLGEFFRTTSPSHAAVIRADARPVV
                     ESEWSRARIEAIGPISEDSQLEPMVITLESGGASGSRPYVRRAEQLAVVLQGTVELTL
                     EENTYSLKRGDAACIPSDIHHCWRNTSRKPAQVLIVTAHRHL"
     gene            complement(9786..10364)
                     /locus_tag="PK614_04405"
     CDS             complement(9786..10364)
                     /locus_tag="PK614_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81482.1"
                     /translation="MNKGEIVQAGPAHEVVNHYLHAGHIQPAVEWLDPTQAPGNDVVR
                     LRAVRVRAESGIITDRFDIRKPIDLEVEFDVVKAGHIFVPVFNLYNEEDVLVFIAHDR
                     DQAWQRTPRPTGRYTSTARIPGNWLAEGMMSVSSLMMTEDPFRMHVHAPRAIGFRVDD
                     SGTGDSARGDFHGRWPGVVRPLLEWRTKYLPA"
BASE COUNT         2066 a         2791 c         3244 g         2307 t
ORIGIN      
        1 tcaggcttcc agaggagtac acatgacgta tcgacatcgg cgcaggaaag tgctgtccgt
       61 actggcaggg atccctatga ttgctgccac gcttttagtc ggcggaatgc agcccgggtc
      121 tcaggctcag gcggcgggtg tccccccggc gtttgcgcag ggattctcag aaatcgtcaa
      181 ggcggtcacg cctgcggtcg tcaacattgc cgtcaccggt ggaggggaag gccgtcggga
      241 aggccgtcgt cagcttcctc caggcggacc attcggtggc ccgcctcctg gacccggtga
      301 tgagccgccc ggcatggaac ccccaggtgg tcccggtgga cctcccggcc ctccgggtgg
      361 tggcccccat cgccccgagc agagtgccgg atccggtgtg atcctcgatc cgaacgggta
      421 tatcgtcacg aataaccacg tggtcgaagg cgcgactcaa atcaccgtga ccctgtcgga
      481 ccgtcgtgaa tttccagcca agatcatcgg aaccgatccg aagacggatt tggccatcat
      541 caagattgaa gccaaagatc tcgcgtccat gaagtgggcc gattatgacg aattgcaggt
      601 cggtgacgtg gtgctagcgg tgggcagccc gttcggactg agttcaaccg tgaccttggg
      661 cattatcagc gctctgggac gcgggaacgt aggcattgcc gattacgaag actttattca
      721 aaccgatgcg gcgatcaacc ccggtaattc cggcggcgct ttggtcaata tgcaaggcaa
      781 gttgatcggc atcaacacag cgatcttctc ccgcaccggc gggtccgagg gcatcggctt
      841 tgccattccg agtagcatcg cgacggacat cgttgagagt ttgacgaaga ccggcaaagt
      901 ggtgcgcgga tggatgggtg tggccattca ggaaattacc ccggcgctgg cgaaatcctt
      961 caaattgccg gagcagcgca aaggcgtgct tatcagcgac gtgaacgaga atgggccctc
     1021 tcattcggcc ggcatgcggc gtggtgacgt ggtcattgcc ttcaatggta aagaagtgca
     1081 gagcgtcagc caattgcgga acctcgtggc ccgtatggct gtcggcaagg atgccgacat
     1141 caagattttg cgcgaaggga aagagcagag cttgaaggtg aaggtggctg agcggnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagcc gccggacaat
     1261 gtcctggcgt ccttgcgcgt tcagatgctt gatgccgcga tgcagagcca gctgaatatt
     1321 cctgcaaaaa ccagcggcgt ggtggtcagt tcggttgagg ccggcagcgc cgccgagtct
     1381 gcagggttgc agcgtggcga tgtgattcaa gaggtcaacc acgaagtggt caagagcctt
     1441 gaggattacc aaaaggcatc ggccaaggtg aagaaagacg aaatggttgt gctcttgctg
     1501 agtcggcagg ggaacaatct gttcgtagcc gtcaacccga aatagacagc aaccagcgga
     1561 cagctttcag ctatcaggcc aattccggct gatggctgaa ggcctcctgc tgaaagcgat
     1621 tttgaatggc tgacgggttc aagtttcaag agtggctgca agatacggtc tttaagccgc
     1681 ttgaagataa aaaaatgccg gtcatggagc acctggtgga gctccaggtc cggctgaccc
     1741 gtgcggtcat tatcacggcg atcatttttg tcggcacatt cttctacgcc gacaccctgg
     1801 tgaagtggat cagggttccg ctccagaata tgttcgttcc cggatctctt tcctggatcc
     1861 ccaccgatct tcccaccgtc cccttcgtgt ttctcgcgcc ggctgaagcg ctgtggcaga
     1921 acgtcaaggt ggccggcctc tttgccctgg tgttggggac tccgtatatt ctcattgagt
     1981 tctggcattt tgtggtgccg ggtctgcacg cgcaggagcg tcgattcgtc ggtccgtttg
     2041 tgatcctgag tacgctggcg ttctacctgg gcctgttgtt ctcgtttttc ttcgtgctcc
     2101 cctttgctct gaactttttg atttcgtatg gcgtgaatgc gggcttcatt ccgcaactgt
     2161 cgattgcgca atatgtcgga ttcgcgctgt ggttccttct ggtcttcggt ctcatttttg
     2221 aagtgccgtt ggtgattacc ctgctggcca aacttggctg ggtggatgcc ccactgctga
     2281 aacgatatcg caagtgggcc ttcctcgcgg cgtttatctt cgccgcgatt ctgacgccga
     2341 cgccggaccc cttcaatcag tgcctgatgg cgctgccgat gtatattttt tatgaggtcg
     2401 ggatcgtgag tgccggaatc ttcaacaaga agaaggcggg cgctcccgaa gaggcgcttg
     2461 tgccggcggt tgccactgcg ggtcccggtc tcgggaagcc gggatcgtcc atgccgtccg
     2521 gcggcggtga tagtgaatat gtcggtgtgc cgactggtcg tcgagggtaa cgcagggcag
     2581 gaatcagcaa cgtgacgaac gcacatgttt gtcccacgca tcatgatata cgctttatga
     2641 ggagtctagg atgccacgcg agttaaacgt catttcacag cccggttcca gcagcggcgg
     2701 agatgcctgc acctatatgt gggcctgcgc catttgcgac gagaatgaaa cctgtcagaa
     2761 agacaaggaa ggccacagcc ggtggctcgt cgccaaacgc atggaacgga tcgaatataa
     2821 ggtcctggtc atgagcaaca agggtggagt ggggaaaagc acctgcacca ccaacctggc
     2881 ggtcagcctc gcgctcaaag gctggcatgt cgggatttgc gatatggaca tccatggtcc
     2941 gaacattccc aagatggtcg gggcggaagg acagaagctg aagatcagca cgtccggggg
     3001 aatcattccg tttcaagcct acaacatgaa gatcgcgtcg atgtcgttcc tgttgcagaa
     3061 ctccgatgat ccgatcatct ggcgcgacgc ttataagtat gagttcatca atcagttgct
     3121 gggaggggtg gaatggcagg atctcaattt cctgttgatc gatctgcccc caggcaccgg
     3181 caacgaatcg gtgacgacta tcgatctctt ggggggggtc agcggagcgg tgatcattac
     3241 gacgccgcaa gaagtcgcgc tgctcgattc ccgcaagtcc gtgaccttct gtaaagacag
     3301 cgaagtgccg attatcggca tcgtcgagaa catgagcggg ctggaatgtc ctcattgcca
     3361 caaggaagtg aatgtcttcc gcaagggcgg cggtgaagcg tcggctactg acatgggcgt
     3421 cccgttcctg ggccggattc cgctcgatcc cgatgtcgtg acacaaagtg atgccggtga
     3481 gccattcgcg ctctttaatt ccgattcggc gaccgcgcag gcctatcacg acatcgcaaa
     3541 tcaggtcgaa gcgttctgca agaagagcgg atcgctggtc aagctggcgc cgcgtcagca
     3601 acattgatgg gacggtgacg cgtgaaagct cccgatgcga caaccggatt gactccgctc
     3661 cacgaagctc agcgcattgt gctggacgcc gcgactccgc tggggcttga aaagatctcg
     3721 attctggata ccctggggcg cgtgctcggc gaagatatca tcgctgagcg ccataatccg
     3781 ccgtgggaca attccgcgat ggacggattc gcggtgcagg cggaggacat tcagcaggag
     3841 cacacgatct ccaagccggt cacgttgacg gtgatcgagg atgtgccggc cggaaagatg
     3901 cccaccaaat ccgtggggcg gggccaagcc atccggatca tgaccggcgc gccgattcca
     3961 aagggcgcgg ataccgtact caaggtcgag gacacggaac atacgcccga ttcggtgcgc
     4021 gtattcaagc cggaaccccg tggctcaaat atccggccgc agggtgagga tgtgaaaaag
     4081 ggcgattgca tcattccaaa aggaacgaca atccgttccg gcgaagcggg gatgctggcc
     4141 attctggcca aatctttcgt gttggtctat cagcggccgc gagtcgcgat tttatcgacc
     4201 ggcgatgagt tggccgatct cgatgagcgc ttcagcgaag agaaaatcat caactcgaac
     4261 agctacggga ttgccgctgc ggtgcaggaa gccggcggcg tgccgatcct gttggggatt
     4321 gcgcgggaca cgccggccgc gttgaaagaa aagatttcgc acggattgaa tgccgatatt
     4381 ctcgtgctct caggcggcgt gtcgatgggg gactacgact tcacgaaagc ggtcttccgg
     4441 gatttaggcg ccgagatgaa tttctggaag ttggcgatcc gcccaggtca gccgcttgcc
     4501 ttcggaaaga ttcagggcaa actggcattc gggttaccgg ggaatcccgt ttcctcgatg
     4561 gtgaccttcg agcaattggt tcggcctgcc atgctgaaac tggccggggc gcgaggatat
     4621 gggcgtccgc tgcttgaggc ggtgtttcag gagaagtttt cgaagcgaac cgatcgccgt
     4681 cattttctcc gcggagtgtt gtggcgggaa gacggggtct tcaaagtccg gacgaccggc
     4741 gatcaggggt caggcattct cacctccatg gtcaaagcca attgtctgat cgacattccc
     4801 gtcgaggcgg aacggatcaa tcccggcgac tcagtcaccg tacagcttct cagtggatcg
     4861 gcatggctgg agcaagcggc cccgatggca tcgaccggtc atcgtccttc ctgttgttga
     4921 gccgcgaaag gaacatgttc atgtccgtgc cgattttatc ttttgtcgga cgatccaata
     4981 gcggtaagac gacgctgatc gagcgtgtca tcccggaact ggtgcgggcg gggtataagg
     5041 tggcgaccgt caaacatgcc gggcatggat tcgatctgga taccgaagga aaagacagct
     5101 ggcggcacaa acaagccggg gcgagcagtg tggtcatcat ttccaagagc agcctggcca
     5161 tgttcgccga tgtgtcggac catatgaacg tcgaggatgt tcgtgaacgg tatctggatg
     5221 cgtcctacga cttgatcctc gctgaaggct ggcggagcga agggtatccc aagatcgtag
     5281 tggtccgcga tcagatcggc gaggtgccgg tttcgcagga tgggctactg gctgtggtgt
     5341 ccaataagcc ggtcgagacc tcggtcccgc tcctcgaccc cgacgatgtc gccggcgtcg
     5401 cggcattgat catccgccac tttcccaaac cccaacggga tgatgcgtaa atcctccgtc
     5461 attgtgctcg gagcgatcgt ggccggcgca gtggctttag ggggagttgc ggttccgctg
     5521 accaaccacc cgaaattttg tgccagctgt cacactatcc gaccggccta tgagcgctgg
     5581 ctcgaatcat cccataaaga ggtggagtgt gtggcgtgcc atgtcaggcc cggcgtctca
     5641 ggttggttgc aggacaaggc gtggcatgga accagggatg tggccatcta tctcttcggc
     5701 aaaccgaccg cgccccacaa tctgcaagcg catgtcgatt cgggcgtgtg tctgagttgc
     5761 catcggaaca tcctccgggt gtcggagatt gcgactcggg atctgcccgc acctgtgaag
     5821 gatgtgggat tggtcatgag ccatcgcaag catatggacg cattcgggaa gcgagggcag
     5881 ggcgagggct gcacgacctg tcacgcagcg gtcgtgcacg agaggccgat caagggctac
     5941 cctatcgtga ttcctcgcgg gcacgtgtcg gccgatagcc ggccctggaa gcctgaacat
     6001 ccggaaggtt ccgtcctgca caagcgggcg caggcggatt gcttccgctg ccacgataat
     6061 aaaaccgagt atgaaggtag agtcctgagc cggaagtgcg agacgtgcca tctgcctgag
     6121 aaactcacag aattcttgtc gttttaatcc acgggaatac tcgctccatg tcgccctcgg
     6181 ccgtcgaagt gcgcaatctg accaaacgct tcggcgggtt cacggcagtg gacgggattt
     6241 cgttcgacat cggccggggt gagattctcg ggctgttggg cccgaatggc gccggaaaaa
     6301 cgacgacgtt tcagatgatg ctgggactgg tcacacccag ttccggctcg atccggatgt
     6361 tcggactcga cttgcagacc aatcgggagg cgattcttca gcaggtgaat ttttcctcca
     6421 cgtacatctc gttgccctat tccctgacgg tggaggaaaa tctcggtgtc atcgctcggt
     6481 tatacgggat gtccaacatc gccggccgaa tcgatgacat ggtgaagaag ctggagatga
     6541 gtgagtggcg aaacaagctc acccgcaaac tttcgtcggg gcagatgacc aggctcaccc
     6601 tggccaaagc gttgctcacg gagcccaagg tgctgtttct cgatgagccc acggcaagcc
     6661 tcgatccgga tattgcgcac aagatccgtg ccatccttct ggaagaacgg caggccaccg
     6721 ggctcagcat tctctacacc tcgcacaaca tgcgggagat ggaggaaatg tcggatcgta
     6781 ttatcttcct gcagcggggc aaaatcgttg cggagggcac ggcgaaggac attgtcgcca
     6841 gattcggaca ggccgatttg gaagaggtgt ttctcaagtt agctcgcgag cagtagcggc
     6901 agatcgggat cgttgcttcg tggaagggag tacgggcagt catggttgac gcaggagtgt
     6961 ccttcggagt gggtctgctc atcggcgggt tgggcctgat gctgggatgg ggattgttgt
     7021 ggctcgtcgt cggcgccatt ggtctggcac gagagacgtg cgggtgggcg atcttcatga
     7081 gcagcgtcag cagcacggtt atcgcggggt tgtcgatggc gggagtgctc tgggtggtgg
     7141 attcggcgag gctaggtagt tctgcgctgc atgccggtct ggccggggtt ccgctggcgc
     7201 tgctcgtcgc agggttctgt cgtttggagg atggccggcg gatcggtccg gcgttcctgg
     7261 aaggcagccg cctcatgttg catcaactgc ttggtgtgca tgaggaggga tgcgggcatt
     7321 gccacgagaa acctgttgag gagccgccct gtcaccagaa gccttgttac gagcagtcct
     7381 gccacgagaa gccttgtgaa gggaagccct agctgccgat ggcctatcat cgtattgcag
     7441 ccctggtgat tcgtcacctg tatctctacc gtcgcagtct cccgcgggtg atggagatca
     7501 tctactggcc gtttctggac ctggtggtct ggggattcat caccgtctat ctggcgactt
     7561 ttcaggggca gatgccggcg gtggtcacgt tcctcctcgg ggcgttgatt ctctgggacg
     7621 tgttatttcg ctcgcagcag ggtattacca tttcgtttct cgaagaaatc tgggcgcgta
     7681 atctgatgaa cctcttcgcc agcccgctca cgccgagcga atttctggcg gccaccatgg
     7741 tgatgagcct gtttaaagtg accgcggtgt cgatggtgat gtcggtctgc gcctggatct
     7801 tttacggcta caacgtcttt atcatcgggc tctggctgat gccgttcatc gtgaatctgg
     7861 tgctgacagg ctggatcatc ggtgtgttca cgacctcgct gatcatgcgg tttgggcagg
     7921 aggcggaagt gttggcctgg agcatggtct ttctgtttca gccgatctcc tgcgtatttt
     7981 atcccatgga ggtcctgccg tcctggctga gagctgtggc ttccatcaat ccggccgccc
     8041 atgtgtttga gggcatgcgc ggcctgctgg ccacccatgc ggctcccctg agcagcctga
     8101 gcctggccag cgggttgaat cttgtgtatc ttggagtggt catcctgtgg tttcatcaca
     8161 ccttcagtgt gtgcaaggac cggggatcgt tggtgcgggt cggtgagtag gtgatcgacc
     8221 gaacagggaa tgtgggagta gtgggatgaa catcaactca gggcaagtca ggccggcact
     8281 gctgccggtc ggtctcgaat ccttttcgtt tcatcagacg ccggtcctcg tcatcgagaa
     8341 tttttggtcc gccgaggagc ggactcagtt tcggcaggcg atgaaccgtg cgaattggaa
     8401 tcagttgcaa gatttgtcgt acgtgcggca ggacttcccc aattccggga actgggcgaa
     8461 ggccgagatc gcccaaccgc aaggccaact cctgctgagc cggttggaga tgccctgcat
     8521 tcagcagtat atcgagtcat ttccgaacat caccaggcgg catctgggtt tcagctatta
     8581 ctcctatggt gccggtgact gccttctgac ccacgatgat acggatcaag gtcgtccggt
     8641 cgctgggaag cccgctcccc gtcgccgtct cgccatggtg agttatttcc atgaagaatg
     8701 ggaatgtgat tggggcggag agttgatgat ttactcagcg accggcgaac caacgaacgg
     8761 aaagccggaa ctggccatta cgcactgcat tgcacccaag ccgggatcat tggtcatgtt
     8821 taccgtgccg cggttccatc gggtgtgccg ggtggatcaa acggcgggag accacaagcg
     8881 cctctcgatt gccggctggt tcatgaccga acatgcctga gtgtgtatcc tgttgcagaa
     8941 caatcgattg cagcgtgagc ggagcacggc aaaatatttt tcagaagttg agaagggcga
     9001 gtggttacaa gtgccgatgg gcggtcacga tgaggacttg ggccggtttt cggctcgtgt
     9061 tgcgccaaca atgatggatg tcagaaggaa tgcaggcggc atcccctcgt ttgaggctgt
     9121 acgtgttttc ttccaatgtc agttcgacgg taccctgaag cacgactgcc aattgttccg
     9181 cgcgccgtac atacggacgt gatccgctgg ccccaccaga ctccagtgta atgaccatgg
     9241 gctccagctg actgtcttcg ctgattggcc cgatcgcttc gattctggcc cgagaccatt
     9301 cgctttccac taccggccgg gcatcggcgc ggatgacagc cgcatgtgat ggactggtcg
     9361 ttctgaaaaa ttctccgagg gttacgccga gcgccgatgc gatccgctcg gtggaggcaa
     9421 tagacggtga ggcctgccgg agttcgacct gggagataaa actaggggag aagccgcatt
     9481 tgtccgctag ggttcggaca gagaggtgtc ggcttttgcg gagacggcgg acgatgtcgc
     9541 cgacatgcgt tttcttccgg tcctcggttg ctacgctctc ctgcggggcc ttcttcttgg
     9601 attttttctc cacctcgtcc tatcctttgg gtcctaccca ggattcgcgc ggttggtgta
     9661 gcacgtttat gggcctgtcg caagccggcc gaaggggcgg aatcttctca gacatatctg
     9721 cggttgtctt ccaataggga gagtgttgtc accatgggac caccctgctg tgtctctgca
     9781 gcctcttatg ccggtagata cttggttctc cactccaaca acggccgaac gactcccggc
     9841 caccgaccgt gaaagtctcc tcgcgccgaa tctccggtcc cgctgtcatc cacacgaaat
     9901 ccaatcgctc ggggcgcatg gacgtgcatg cggaaaggat cttcggtcat catcagggat
     9961 gagaccgaca tcattccttc ggcaagccag tttcctggaa ttctggccgt ggaagtatat
    10021 cgaccggtcg ggcgaggggt tcgctgccag gcctggtccc ggtcatgggc gataaaaaca
    10081 aggacatctt cctcattgta gagattaaag accggcacaa agatatgccc ggctttcacg
    10141 acatcaaatt ctacttcgag atcaatcggt tttcgaatgt cgaagcggtc cgtgatgatg
    10201 ccggactcag cccggacgcg tacggcgcgc aagcggacga cgtcattgcc cggagcctgg
    10261 gttggatcca gccattcgac cgcaggctgg atgtgtccgg catggaggta gtgattcacc
    10321 acctcatgcg ccggccctgc ctgcacgatc tcccccttgt tcatcgacac gccggtgaaa
    10381 cattattcca gcggcatgta tgtccggtta gggttctccg tgctggccca tatggatccg
    10441 gacattctga tcgtcgat
//
LOCUS       DAORQN010000044        10203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_360_length_10203_cov_31.691565, whole genome shotgun sequence.
ACCESSION   DAORQN010000044 DAORQN010000000
VERSION     DAORQN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_10203_cov_31.691565"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..365)
                     /locus_tag="PK614_04410"
     CDS             complement(147..365)
                     /locus_tag="PK614_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81483.1"
                     /translation="MIDAARRWKAWATAGCAEAPAQNHKLVLAQHQHGMAEQIQRIRG
                     EGPVQRDRSDQAHAPQWKGVSKSASRRS"
     gene            534..776
                     /locus_tag="PK614_04415"
     CDS             534..776
                     /locus_tag="PK614_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81484.1"
                     /translation="MAPQDKKPQPVTTLRCSRIKASIWKNEGMNGSFYNVTVARSYKD
                     RDGIWKNSESFGLADLDALVAVAQQAKLWVTERSDR"
     gene            complement(754..933)
                     /locus_tag="PK614_04420"
     CDS             complement(754..933)
                     /locus_tag="PK614_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81485.1"
                     /translation="MADSQERDADGMQAVNQLIEVLPQFLITLHRLTFFLRIQEAPDP
                     RAGGFHTAVSDPTAP"
     gene            complement(975..1418)
                     /locus_tag="PK614_04425"
     CDS             complement(975..1418)
                     /locus_tag="PK614_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81486.1"
                     /translation="MFVQPSSLSRLLTLSFLLGVSSVSAGDPEQVSVQTLFSPRVLSY
                     QQHAVTVEGVIQNLQVFSPFDRTNGRSAPAKCLLYGRAAFLLEDETGIIPVEVLGTCH
                     PHVVELLPRNGDHVRITGLVQVLKSEAPRHVRIQAMTIQILESIP"
     gene            complement(1529..2002)
                     /locus_tag="PK614_04430"
     CDS             complement(1529..2002)
                     /locus_tag="PK614_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81487.1"
                     /translation="MSMHPLTRSAIRGLMVLLPVATASVSPAQFVPRSKPPAFHSDAV
                     TSIHAILSDPGHYQFRLVRIRGVVQSIIQIPNWIKCGFAPAYKIRVEDESGGLTVIDQ
                     GPCGRGNRGPLFPETLAGGERIDALIFVSFTNQPRESPNPPEGLLQWLEQTPSQD"
     gene            2392..2943
                     /locus_tag="PK614_04435"
     CDS             2392..2943
                     /locus_tag="PK614_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81488.1"
                     /translation="MGQTTKRMTTVTVDLGELKTAWLAWCQQQGVTPSHALRNALRRA
                     MDRRATRAPPPRSRVSPKRERATARIELNLTTSELAALKKMAGHEGYMPTKWMVAMVR
                     TKLTGQPQVGQPELETLARSNQQLLALGRNLNQIAKVFNTAPQNRTAFRVEVVTELSR
                     VIQAHTKKVSDVLRGTVERWHIQ"
     gene            2940..3923
                     /locus_tag="PK614_04440"
     CDS             2940..3923
                     /locus_tag="PK614_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HRB81489.1"
                     /translation="MSSTGSQIDQKLDEWGSRLFNVSTETLPRPRFSKHVRLASTKPV
                     GTGGRFSTPQARATYVRQKLQAMVRRAPQVVVKLVRAPKGMKGISNNLTYISRDGQLE
                     IEDQDGQVIQGKEAVADLKAEWRDGGMPIAADSTMRDAFHLVLSMPTSTDPLSVQRAA
                     RDFATREFGGFQYAMVLHTFETDPDPHPSPHPHVHLTVKAAGLDGVRLNPRKVDLQRW
                     REGFAEALREHGIEATTTSRIHRITHDRWTVRHLPEPSKKGETLERLKRTTGRQGRTQ
                     DVMRNYEQVMRTLARSDRGEDRQLAADLVRYLGARGRTPETDRERGKDRER"
     gene            3920..4111
                     /locus_tag="PK614_04445"
     CDS             3920..4111
                     /locus_tag="PK614_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81490.1"
                     /translation="MRRLVIDDTGKDNISTSTVVLLNDANHTRVQKMMRHQHYATTEI
                     YVEEVQRLLEGAEDAVTQI"
     gene            4475..5080
                     /locus_tag="PK614_04450"
     CDS             4475..5080
                     /locus_tag="PK614_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81491.1"
                     /translation="MAFGVQIPQSTRILGLLVVTFLLIGCASTTVTTTGLPLKEPLCQ
                     PSKPSIPTVVYWGTKWRPDQKEPKLREAAAMRGIQDFLSRSGCLLVLTVNRLAPEQAQ
                     ASNEELMHWASSANPKPDRIVRIVVRELGPRLLIGIPVIVEGGTEVVVEVNVLDAMTS
                     QLLANTQTTWRNGGPFVIKGIKTLEQDMSAALAATLMVNGH"
     gene            5182..5913
                     /locus_tag="PK614_04455"
     CDS             5182..5913
                     /locus_tag="PK614_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB81492.1"
                     /translation="MTEQIDSRHVEYALTRNGQEIVVFENGLGGTMHWWAKVFPEIAK
                     TTTSFAYNRPGYGKSDPASTPRDGQHIVNELRSLLRSKGLNPPYVLVGHSLGGLYTQL
                     FARRYPHEVQGLVLVDSTHPLQLKGAGSPENWPTWVRVVFSLATSAVAKEELTAIDPT
                     GEEILGLPTVGNKPMIILSALRPMKGTSALAKDGNEKRKDLARLYPDARQIWVNSDHG
                     IPLENPDSVIEAVREVLSQLQPSLP"
     gene            6003..6653
                     /locus_tag="PK614_04460"
     CDS             6003..6653
                     /locus_tag="PK614_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HRB81493.1"
                     /translation="MKFSPHHAAGFAGLGATVLVLVGTWAFALARPEYSHIRHTISEL
                     GESGAPLASLVSFGFFLPTGVLVWLAIVLAYPCYARDRSTYLGLCAFASLGLGYVMAA
                     FFPCDPGSPLMGSWQQQVHNVFGLVEYLGTGAALIAFGWSQTKAKSYLTGLALVGSGV
                     AVFIGLVLLSTPSWVSVRGLVQRITEAIIFGWLTLASLSLIFRCTQPNQALKWYSF"
     gene            7015..8175
                     /locus_tag="PK614_04465"
     CDS             7015..8175
                     /locus_tag="PK614_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81494.1"
                     /translation="MKLEKHLFSMILGICLVATEAFAQSALVHLQGETGSREAYFANF
                     AVVLDRTPTDKLLGPTRVRQLDTTIIYESADKPEFSELRLQFECVAKHAYDGKNVPKQ
                     PALDAPVNVRVGEGSWKLRRQDIKSEDLPAGDWRTSSSPVLLKLHKIACNEDVMRNAM
                     IKAAKENNNMDIFRNEIVKIGLPKDLQLVGQQVSVEYMQFAWSVLWNGAKRPDPTGGW
                     SRRPTKKQEEEFRFKMDQMQKQYDQIAAGVTPKLEASIKEMDSESAFEKTAADIRGGR
                     TLSKNEFHMLTVWKGKTETDVGATLGAPIVSEAGKLRFLSYGKEFDNRVVVGNMQGAV
                     WQEGFRENCNVQFVMHQDAKSVSRVVDVRIWASSSRVGQVVFACDGLLEVPK"
     gene            8187..8825
                     /locus_tag="PK614_04470"
     CDS             8187..8825
                     /locus_tag="PK614_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81495.1"
                     /translation="MRKIVALLLCLVGALAYAESYDPMDDLVSTAKKEINAQEAIAAS
                     KDRVLNDPKREKVEKGFWKVFQGKSGAKPGEFCAAVYWQHDRMIIISGPGGDYKGAML
                     GFVALIPDQSFPRANNPSERQKVKVTLTQGNDSPATVTAFNSIIGDRSGQIQFPVPTI
                     DAALAGMDDKVNFRIDYENKNIFELEWHSAFAAREALKKCLKGENVQGKEVL"
     gene            9339..9818
                     /locus_tag="PK614_04475"
     CDS             9339..9818
                     /locus_tag="PK614_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81496.1"
                     /translation="MGIQPTSAYRAPSLIGNILQHRHNSADNTAMLAASATNAADMTT
                     ISQAARNRAAEETLGGATYDFTNMSPNDILPTVNSLIKSGQMSLDESDSLILLVPISL
                     GGDPVPPAALSQKRNLFSDLEDWLSSNRYLHNDVGAEADQRAISALKRIQALHQTEG"
BASE COUNT         2322 a         2616 c         2987 g         2278 t
ORIGIN      
        1 accgctcgtc tctgatactg gcccattctt ttgtcaccat ggttgagctt ccccggcaac
       61 ctcgcgaaag ctgttcggtc ttccactgcg ttcagcgacc atctggcacg tagtcggtcg
      121 cttgctggtc gacaaccgcg tcaagctcaa gaccgtctcg aagccgactt gctgactcct
      181 ttccactgcg gcgcatgggc ttggtcgctt cggtctctct gaacaggacc ttctcccctg
      241 attcgttgga tctgctctgc catgccgtgc tggtgctgag cgaggaccag cttgtggttc
      301 tgggccggtg cctctgcgca tccggccgtc gcccaggctt tccaccggcg ggctgcgtcg
      361 atcatgcccg cgcgccgcct ctgaagcggc tcctttccgc attcattcct ccgcttcgcg
      421 cctgccggcc cgccaggtgc cgacggcctc tctgtctcct ttgtcgtcgc tgtgtggcct
      481 ccactgcctc gtgagaggcc aacacatcaa ctctaactca aggagacaac actatggcac
      541 ctcaagacaa gaaaccccag ccggtcacca cgcttcgatg cagccgcatc aaggcgagta
      601 tctggaagaa cgagggcatg aacggttcgt tctacaacgt caccgtcgcc cgctcataca
      661 aggaccggga tggaatttgg aagaattccg aatccttcgg cctggcggac ctggatgcgc
      721 tggtcgcggt cgcccaacag gcgaaactct gggtcacgga gcggtcggat cgctgaccgc
      781 agtgtggaag cctccggccc tcgggtcggg ggcttcctgt atccgaagaa agaacgtaag
      841 acgatgaaga gttattagaa attggggaag cacctcgatc agctgattga cggcctgcat
      901 gccgtccgca tcacgttcct ggctatctgc cattgtctgt gagtgctcgc ctcgcatcgc
      961 gcagaggctc agcgttaggg aatcgattcc agaatctgaa tcgtcatcgc ctggattcgt
     1021 acgtgacgag gagcctcact cttcagcacc tggacgagcc ccgtgatacg gacatgatct
     1081 ccattgcgcg gcagtaattc caccacgtgt ggatgacagg ttcccaacac ttccacgggg
     1141 atgatccccg tctcatcttc gaggaggaag gcggctcgac catagagcag acattttgcg
     1201 ggagccgatc gaccattcgt gcgatcaaat ggggagaaca cctggaggtt ttgtatcacc
     1261 ccctcgacgg tcacggcatg ttgttggtag gagaggactc gtggcgagaa caaggtctga
     1321 accgagacct gttctgggtc accggcactg acgctgctga cacccaggag gaaactcagc
     1381 gtgagcagtc tgcttagcga cgacggctgt acgaacatgc cttctcctgc tgtcccaagt
     1441 ataccgctcg cacgggagga cgagcggacc ctttcgtccc cctcgtggag tgttccggtt
     1501 ccgagaatgt tgaaaagaaa agaagtgttc agtcttgtga tggggtttgt tcaagccact
     1561 ggaggagccc ctcaggtggg ttgggagact cgcgtggctg atttgtgaac gagacaaata
     1621 tgagggcatc aatccgttca ccaccggcaa gtgtttccgg gaacagtggt cctctgttcc
     1681 ctcgtccaca gggtccttga tcgatcaccg tcagtccgcc agattcgtcc tccacccgta
     1741 tcttatacgc gggcgcaaaa ccgcatttga tccagttcgg gatctgaata atggattgca
     1801 cgacgccgcg gatccggacg agtcggaact gatagtgtcc gggatccgac agaatcgcgt
     1861 gaatggaggt cacggcatcc gagtgaaaag cgggcggttt cgatcgcgga acgaattgtg
     1921 cgggactgac gctggccgtc gcgacgggca gcaggaccat cagcccccgg attgcagagc
     1981 gtgtcagggg gtgcatactc acagtatagc gcctccagat tatgtgagat cgtgcgagac
     2041 aatgccggta tgcccggctg gccgggggct ccaccgaccg gccgatccgg gcccgtttct
     2101 ggcgtcccgt gcacagcgtg ccaggttgat cggtggtctt ggatccgtgt cgacatgggc
     2161 acaatgtaat accgatttta agaggttggg agggcagagc tcttttactt ctttcctctc
     2221 ttcttatcct tatttttgat gttgcaggat atggctgttg cccgtagttg tcttacattc
     2281 tgggtttatc gtattcttgt gcgcctgttc tttggccctc cgatgacccg aatcatccac
     2341 aggtcggtga ggggaatggg ctgtcttaat tgggcagggg taggtgaact gatggggcag
     2401 acgacgaaac gcatgaccac ggtcacggtt gatttgggtg agctcaaaac ggcatggctg
     2461 gcctggtgtc agcagcaggg tgtcaccccg agccatgccc tgcggaacgc ccttcggcgg
     2521 gccatggacc ggagagccac gcgggctcct ccgcctcgga gtcgcgttag cccgaaacga
     2581 gagcgagcga ccgctcgtat cgaactcaac ctcacgacct cagaactcgc cgccctaaag
     2641 aagatggccg gtcacgaagg ctatatgccg acgaaatgga tggtggccat ggtgcggacc
     2701 aagttgaccg gacagccgca ggtcgggcag ccggaattgg agaccttggc acggtcgaat
     2761 cagcaactgc tcgccttggg gcggaacctg aaccagatag cgaaagtctt caataccgcg
     2821 ccgcaaaacc ggaccgcctt tcgggtcgag gtcgtcaccg aactctcccg cgtgatccaa
     2881 gcccatacga agaaggtgtc cgatgtcttg cgcgggaccg tggagcgctg gcacatccaa
     2941 tgagttcgac aggcagccag attgaccaga aattggatga gtgggggagt cggcttttca
     3001 atgtctcaac ggagaccctc ccgcgaccac gtttcagtaa gcacgttcgt cttgcttcca
     3061 ctaagcctgt gggtactggc ggacggttca gcacccccca agctcgggcg acctatgtgc
     3121 ggcagaaact gcaggccatg gtccgccgcg caccacaagt ggtggtgaaa ctcgtccggg
     3181 cgccgaaagg gatgaagggg atctcgaata acctcaccta catttcgcgg gatggccaac
     3241 tcgagatcga ggatcaagat ggtcaggtga ttcaggggaa ggaggccgtg gcggatctga
     3301 aagccgaatg gcgcgatggc gggatgccga tcgcggcgga ttcgacgatg cgcgatgcct
     3361 ttcatctcgt cctctccatg ccgaccagta ccgatccgtt gtccgtccaa cgcgcggccc
     3421 gtgactttgc gacacgagag tttggagggt tccagtacgc gatggtcctc cacaccttcg
     3481 agaccgatcc ggatccgcat ccctctccgc atccgcatgt ccacttaaca gtcaaagccg
     3541 cgggacttga tggagtccgc ttgaatccga gaaaggtcga tctgcaacgg tggcgggagg
     3601 ggtttgcaga ggcgttacga gagcatggca ttgaggccac gaccacgagc cgaattcacc
     3661 ggatcacaca tgaccgctgg accgtgcgac atctgcctga accgagcaaa aaaggagaga
     3721 cactggagcg gctgaaacgc accacgggcc gacagggacg aacccaggac gtcatgcgga
     3781 attatgagca ggtgatgcgg acgttagcca ggtcagatcg tggggaggat cggcaactgg
     3841 cggctgatct cgtccgctat ctgggggcac gaggccggac tccggagaca gacagggagc
     3901 gagggaaaga tcgtgaacga tgagacggtt agtcattgac gacaccggca aagacaatat
     3961 atcgacctcg acagttgtcc tcctcaatga tgccaatcac acgcgggtgc agaagatgat
     4021 gcggcatcag cactatgcga ccaccgaaat ttatgtggag gaagtgcaac gattgctcga
     4081 gggggccgaa gacgcggtga cgcagattta gatggcaaga tgggaggcgg tttcgcacag
     4141 tcatcgccct ggtaatccag ggctgcctca cagttttagc tggattgcca gatacggctc
     4201 atattccatg gggacgggtg acgcgcagca ggacgatcat cgccgggcta gcaaccgttc
     4261 gagttgaaga tgtgcgtgtt cttgtggagc tggttggggc ggggaattca aggaaatcat
     4321 cgatcgatgc catccattcg aggagatcat cgacacccgc ggttatggcg acacgcgcca
     4381 taggaaggaa catgtcgtga ttacttcaaa gcatgacgat tacagccagc aataccgatc
     4441 acatagacga ccatttccag ggccatggtc catcatggcg ttcggcgttc agatccccca
     4501 atccacgcgc attctcggat tactcgttgt tacctttctc ctgatagggt gcgcatcgac
     4561 cacggtgacc acgacgggct tgccgctcaa agagccgttg tgccaaccta gtaaaccatc
     4621 gattccaaca gtcgtctact ggggcaccaa gtggagacct gatcaaaaag aaccgaagct
     4681 ccgcgaagct gccgcgatgc gtggaatcca agacttcctg agccgatcag gttgcctctt
     4741 ggttttgact gtgaatcgcc ttgcccctga gcaggcgcag gcgtccaatg aagaactcat
     4801 gcactgggcc tcaagcgcga atcccaagcc agaccggatt gtgcgtattg ttgtgcgaga
     4861 gttgggccca aggctgctta tcggaatacc cgtcattgtg gaaggcggca ccgaggttgt
     4921 cgttgaggtg aatgttctcg atgccatgac atcgcagtta ctggccaata cacaaaccac
     4981 atggcggaac ggcggtccct tcgtgatcaa gggtatcaag acactcgaac aagacatgag
     5041 tgcggcactg gctgctacac tcatggtcaa cggccattga tcgatccgag gtcaataacc
     5101 atgcgaatga atagacctgc cccatatgtt ttatggttct gtgcgatgat tgttactgga
     5161 tgtgcgtcat tgccgaatac cgtgacggaa cagatcgata gccgacatgt ggaatatgcc
     5221 ctcacgagaa acggtcagga gatcgttgtg tttgaaaatg gtctgggtgg aaccatgcac
     5281 tggtgggcta aggtgtttcc agagattgcg aagaccacca cgtcgttcgc ctataatcgt
     5341 ccaggctacg ggaaaagtga tcccgcttcc acgccgcgtg acggtcagca catcgtcaat
     5401 gaactgcggt cattgcttcg aagtaaaggg ctcaatcccc cgtatgtgct ggttggacat
     5461 tcgttaggag gtttgtatac gcagctcttt gctcggcgtt atccacacga ggttcagggg
     5521 ctagtcctcg tggattccac ccacccgttg caactgaaag gcgccggctc gcccgagaac
     5581 tggcctacat gggtacgagt tgtgttcagc ctggcgactt cagcagtggc aaaagaggaa
     5641 ttgactgcca tagatccaac gggagaagag atcttgggtt taccgaccgt cggtaacaaa
     5701 cctatgatca ttttgagcgc cttacgaccg atgaagggaa cgtcggcttt agcgaaagat
     5761 ggaaatgaaa agcggaaaga cctcgcgcga ctgtatccag atgctaggca aatatgggtg
     5821 aatagcgatc atggcatacc gttggaaaat cctgactcgg ttatcgaggc ggtccgcgaa
     5881 gtcctgtctc aactgcaacc cagcctgccg tagaccggcg ggatgagatc actcagcaag
     5941 acgatagtcc ctccgcggcc aagagccacg tatgagatct gtccccgtca gatagcaata
     6001 tcatgaagtt ttcccctcat catgcagcgg gatttgctgg acttggtgcg accgtgcttg
     6061 tgctggtggg cacgtgggcg ttcgcgttgg caagacctga gtatagccat atccgtcata
     6121 cgatcagtga acttggcgag tctggtgctc ccttggcgag cctcgtcagt tttgggtttt
     6181 ttcttcccac aggcgtcctg gtttggctgg cgattgtttt ggcctatccg tgctatgcac
     6241 gtgatcgatc aacctacttg ggtctgtgcg ccttcgcctc gctgggactt gggtatgtga
     6301 tggcggcctt ctttccctgt gatcctggtt ccccgctgat gggttcctgg caacagcaag
     6361 tccataatgt atttggcttg gtcgaatatc ttggaacggg cgctgctctg atcgcatttg
     6421 ggtggtcgca gacaaaggca aaatcctacc tcaccggtct ggcgcttgtc ggctcgggcg
     6481 tggcggtatt catcggtctt gttctgcttt ccacgccgag ttgggttagt gtgagaggac
     6541 tcgttcagcg aatcaccgaa gcgattatat tcgggtggct gaccttggca agcctatcct
     6601 tgatcttccg gtgtactcag ccaaaccaag cgttgaagtg gtacagcttc tgatcgttgc
     6661 tgcccttaag ctggtgacaa ggttttatag gcttgaggtc aaaagccgcg actctaagtt
     6721 gccttggcgt cgtgaatcga catcatagat atccaaccct gtcatcaaga ttctcatgtt
     6781 cgcttcgcgt aagcctgaga ttttcgaata gttataggcg ctgggagtac gcaatggctt
     6841 gattagaaga ctattgtccg gcgttcttgc gatagtgtct ttgttgaccg cggctcattt
     6901 gtggaatctc ctcgccttgc ctatagatca actatagact cctttgtatt tggcagaata
     6961 ctgtgaacgg aaggaatact tcatagagct tctacccaac aaggggcacg caagatgaaa
     7021 ttggagaaac acctattcag tatgattctt ggaatctgcc tggtcgcaac agaggccttc
     7081 gctcaatctg cgttggttca cctccaaggc gagacaggca gccgcgaagc ctattttgcc
     7141 aacttcgcgg tggtcctgga tcgaacacct acggacaagc ttcttggccc cactcgtgtg
     7201 aggcaattgg atacgacgat catctacgag agcgctgaca agccggagtt tagtgagttg
     7261 cgtcttcagt ttgagtgtgt cgcgaagcat gcctacgacg gcaagaatgt tccgaagcag
     7321 ccagcgttgg atgcgcccgt gaacgtgcgg gtaggggagg gcagctggaa gcttcgccgc
     7381 caggatatca agagcgagga tcttcctgct ggggattggc gtacgtcgtc atcgcctgtg
     7441 ttgcttaagc ttcacaagat tgcttgcaat gaggatgtga tgcggaacgc gatgatcaag
     7501 gcggccaaag agaataacaa tatggacata ttcaggaacg agatcgtgaa gatcggactt
     7561 cctaaagacc tgcaattggt tggtcaacag gtgtcggtcg aatacatgca atttgcctgg
     7621 tcggtcttat ggaacggggc taaacgcccg gatcccaccg gagggtggtc taggcgccca
     7681 accaagaagc aggaagaaga gtttcgtttc aaaatggatc aaatgcaaaa gcaatacgat
     7741 cagattgcgg caggggttac gccgaaactc gaagctagca tcaaggaaat ggacagcgaa
     7801 tcagcgtttg agaagactgc cgcagacatt cggggtggga gaaccctgtc aaagaatgaa
     7861 ttccatatgt tgacggtgtg gaaagggaaa acggagaccg atgtgggggc gacgttgggg
     7921 gctccaattg tgtccgaggc tggtaaattg cgatttctca gttatggaaa agaattcgac
     7981 aatcgggtgg ttgtgggcaa tatgcaaggt gccgtgtggc aggagggttt tcgagagaac
     8041 tgtaacgtgc agtttgtcat gcaccaagat gcaaaaagtg tgtcgcgggt ggtcgatgtg
     8101 cggatctggg cgagttcgag tcgggtcggg caagtagtgt ttgcctgcga tgggctcttg
     8161 gaagttccta aataagagga ttcgatatga gaaagattgt agcgttgtta ttgtgtttag
     8221 ttggtgctct cgcgtatgcg gaatcctatg acccgatgga cgatttggtc tcgaccgcca
     8281 aaaaggagat caatgcgcag gaggccatcg ctgcatcgaa ggatcgcgta ctgaatgacc
     8341 caaaacggga aaaagttgag aaagggtttt ggaaagtgtt tcaagggaaa agcggtgcca
     8401 agccgggtga gttttgcgca gcggtgtatt ggcaacacga caggatgatt ataatttcag
     8461 ggcctggtgg tgactataag ggtgccatgc tgggttttgt cgccttgata ccggatcagt
     8521 cgttcccacg agcgaataat cccagcgaac ggcagaaggt caaagtcaca ttaactcagg
     8581 gcaacgattc ccccgctacc gtgaccgcgt tcaattcaat catcggcgat aggtctggtc
     8641 agatacagtt cccagtcccc accatcgatg ccgcgttagc tggcatggat gataaagtga
     8701 acttcagaat cgactacgaa aacaaaaata tttttgagtt agagtggcac tcggcctttg
     8761 ctgcacgcga agcgttgaag aaatgcctga agggcgagaa tgtgcaaggg aaagaagtgt
     8821 tatgacagca atgcttccat cattcatagt caaggtgatg gcggaagaca atgggctggc
     8881 agaggggctt gagtagtatc tgcgcactcg ctgccctgcc aggagtgtct cctgcaacga
     8941 ccttcgggcg tcattctcgc tgagtactcc acagatccca tctcgtctag ttattcctta
     9001 aacttctgca gtgcatggtt tcggaaggct cccagacgcg tataggcaaa ggacgaccag
     9061 aagtcccgtt aaggagtgag tgaaagaatg gtccttgccc attctggcag gttgcaacgc
     9121 agatcccgat gagaacccat gatgcctggt gtcagcggtg cgatacacgg aagtagcaat
     9181 agttgttgca gactggtcac agctggagtg gtgtgtgatc cagacagaag gtgcctgact
     9241 cggcggtagc cttttcatag ctgatgggtg ggacacgagt aaggcatggt agttgctcga
     9301 atgtgagagt gcgccaatat catcttatgg ggtatcacat gggaattcaa ccaaccagcg
     9361 cctatagagc tccatccttg atcggtaata tattgcagca ccggcacaat tctgccgaca
     9421 acacagcaat gcttgccgca tcggcgacaa atgctgccga tatgacgact atctcgcagg
     9481 ctgcgcgcaa ccgcgcagca gaagaaacct tgggtggtgc cacctatgat tttacgaata
     9541 tgtccccgaa cgacatactg cccactgtta atagcctcat taagagtgga caaatgtcgc
     9601 tggatgagtc cgattccctc atcctcttgg tccccatctc tctaggaggc gatcctgtac
     9661 cgccggccgc tctcagtcag aaaagaaatc tattctcaga tctggaggat tggttgtcgt
     9721 ccaataggta ccttcataat gacgttggcg ctgaggcaga tcaacgagcg atttctgcac
     9781 tgaaacgaat tcaagccttg catcaaacag aaggctgatc atgtggggtg agccgatctg
     9841 cgaaaagtta cccgggtgga tgtagtggag atgatgtcac cgagtgtcct gtttctccca
     9901 accctaagcg agtacgggag gcttgagctg atcgagcgga ttcagacggg cgcgtggtcg
     9961 atcataagct tcaatctgac gccatcacaa cctaagcgtc tcgaccttca tccacgttgg
    10021 gaaatactct cgaggaggcg gtctcatcat catacaacaa cagagatttc tgtgaagaaa
    10081 gcatagaagc ggctggaggg agcggaggag atggtgacgg aacatagatt ggggtaagat
    10141 tcttgaccca cgaatcccaa ggtggggtca ccgtcatgta cagctgcacg agctctgagc
    10201 ggg
//
LOCUS       DAORQN010000045        10050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_365_length_10050_cov_8.366683, whole genome shotgun sequence.
ACCESSION   DAORQN010000045 DAORQN010000000
VERSION     DAORQN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_365_length_10050_cov_8.366683"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /gene="ssb"
                     /locus_tag="PK614_04480"
     CDS             complement(<1..171)
                     /gene="ssb"
                     /locus_tag="PK614_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB81497.1"
                     /translation="MPPWSHHKEPPLAGFNKVILVGNLTRNPELRYTPSGTPVASFGL
                     ATSRRFKQGDELK"
     gene            complement(122..475)
                     /gene="rpsF"
                     /locus_tag="PK614_04485"
     CDS             complement(122..475)
                     /gene="rpsF"
                     /locus_tag="PK614_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRB81498.1"
                     /translation="MELYESLFIIRPTLSDEETTALIEKMKGTVTKNGASLERAENWG
                     RKKLAYEIKRERKGTYVYFYFKGPGAVIAELERAYRLEDSIIKFLTVKLEQEPAPPRG
                     APVVAPQGATVGGVQ"
     gene            complement(720..1085)
                     /locus_tag="PK614_04490"
     CDS             complement(720..1085)
                     /locus_tag="PK614_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81499.1"
                     /translation="MTRLVPGFIALCVILLPAGCAQSVHQAERLAQSDFLVDPGYAGS
                     QSYSYWPGPGYGPGYGIGYGPGYWGSPLGYYGGPYGPLGFGWYGGYGGFGAARPPSGT
                     PGPRPSLPPHAPPQFKKKL"
     gene            complement(1150..1452)
                     /locus_tag="PK614_04495"
     CDS             complement(1150..1452)
                     /locus_tag="PK614_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81500.1"
                     /translation="MTRSIGSLFVASALLLLTACAQSFHQGERLGSVGAHMNPGYPES
                     PSYWNGPGFWWNPFGYYGSGYGWYGGGYGIGSQQSSAPAPRPTPPANAPPQFQKKY"
     gene            complement(1464..2105)
                     /locus_tag="PK614_04500"
     CDS             complement(1464..2105)
                     /locus_tag="PK614_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB81501.1"
                     /translation="MVWTRFVCSRLSAGLLGCVLLLCSPSPIRAEWYVAAQLGANFAD
                     PLRNVRGSGTLAGLDAPNFNLKTSPAFGGKLGVFPGHGLFGIELDVSHSTPHIKNLDD
                     VPGIHLSVTNIGAHVVLRYPGVTWQPYIGGGPALLVARLGRSSTTESDTQVSVGGNVL
                     VGIRAFVTPKVALFTEYKYTDSTFRFGGAFGPVGGFDATYRAHQLFVGLSYHF"
     gene            complement(2268..2480)
                     /locus_tag="PK614_04505"
     CDS             complement(2268..2480)
                     /locus_tag="PK614_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81502.1"
                     /translation="MRKPGEPIYLWIHLLALLLVIIATVALPRAAEFVVGPLSFGTRA
                     LAGVGIAVAGGIALYLLYNSSARNEP"
     gene            complement(2570..3775)
                     /locus_tag="PK614_04510"
     CDS             complement(2570..3775)
                     /locus_tag="PK614_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81503.1"
                     /translation="MTTSSHQSLSLAQFDLSPDRGFLPATDPLAQLPDEPILNQIGAE
                     LPKFLTARQFRSFVQDHQEVMGPVADDWGLDVFRCAMRTLSFAGHAFVWEDPDHPASL
                     IPASLAMPWCAVAQQLGRPPVLSYASYALHNWRRLDPQKPIELGNVVLLQNFLGGLDE
                     EWFVLIHIDIEAKAGPGLVGLVQAHNAARANKPDDVLQGLHALAAAQEAMRETLLRMP
                     ERCDPYIYYSRVRPYIHGWKNSPSLPQGVVYEGVADYAQRPQQFRGETGSQSSIVPAL
                     DAGLGIPHADDPLTQYLVEMRDYMPPRHRTFLAALEARTDEQHRPLLYGYARDRKATH
                     PDLWKAFCTCVDLLAQFRDIHIGYAERYIFQQHQSHASNPTAVGTGGTPFMPYLSKHL
                     EETKRFTLD"
     gene            complement(3903..4493)
                     /gene="pth"
                     /locus_tag="PK614_04515"
     CDS             complement(3903..4493)
                     /gene="pth"
                     /locus_tag="PK614_04515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRB81504.1"
                     /translation="MRLLVGLGNPGADYADTRHNIGCWVIERAAARWSIRLSRKGAAQ
                     RGSGRAGSKLVELASTLDWMNLSGPPLKGLLRELGLTPEALVVVHDDLDLEPGRVRIR
                     QDGGSGGHNGIKSVIEALGTPQFVRLKIGVGRPAPRQDTADYVREQVSPEERAIYAPC
                     LERAVDALELLLNRDVATAMNQFNVREKAEGGERPS"
     gene            complement(4499..5266)
                     /locus_tag="PK614_04520"
     CDS             complement(4499..5266)
                     /locus_tag="PK614_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRB81505.1"
                     /translation="MKFDLTVESREGGGKGPARQLRRAGRVPAVLYGQGECLLLSVKP
                     DELVKILRSQAGSTALISLAINGAKSKAKRTALLREFQVDPITGSVLHADFFEVAMDK
                     PIRVKVPVHLTGGQPLGLKEGGVLHHVLRQLHIECLPSVLPDFIEVDASQLQINQGIH
                     LKDIPKTEGLRFLDDLEHMVVSVAAPMTDAKLEALLTAGAPGAEGAKEPEVAAKGKAV
                     AEGAAGAEAGKAGDAKAGAAAPKAGAAPAKKEAEKKK"
     gene            complement(5304..6245)
                     /locus_tag="PK614_04525"
     CDS             complement(5304..6245)
                     /locus_tag="PK614_04525"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRB81506.1"
                     /translation="MNRELKLFSGSANPALARAICSYLGLPLGAATVSSFSDGEIRIR
                     VEENVRGADVFVTQSCCSPVNDSIMEMLIMIDALKRSSANRITAVIPYFGYARQDRKD
                     QPRVPISAKLVADIITTAGADRVLTMDLHASQIQGFFNIPVDNLYALPVLMDYITKRK
                     VSDLVVVSPDAGGVERARAFAKRLQANLAIIDKRREGPNQAQVMNIIGDVQGKSALLL
                     DDMIDTAGTIVQGAQACLDKGAREVWAGCSHGVLSGPALERLQQSGLTEVLVTDSIPL
                     RGKEQKCPKLKVLSVAPLFGEAIRRIHEDESVSSLFV"
     gene            complement(6544..7443)
                     /gene="ispE"
                     /locus_tag="PK614_04530"
     CDS             complement(6544..7443)
                     /gene="ispE"
                     /locus_tag="PK614_04530"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRB81507.1"
                     /translation="MNSTSLRVLTPAKVNLILRVLDRRPDGFHAVWSLMHTVGLEDEL
                     TLTVQPGTTSRIALRCDHVALAADQSNLVYRAAQLVLARIDRAVDLSITLTKRIPLGA
                     GLGGGSSDAAATILGLTRLLGLNWPIEQMAEVGQQLGSDVPFFFMGPAACVTGRGEQV
                     RPIQMPGTRWIVLVNPGFPVETKWAYQQLSATRAGVRPLSPALQQLGSRAAVDWSEII
                     PLVENDFEAPVFLQHPVLAQIKSQLLSQGAEVALLSGSGATMFGVFPGQADAERAASV
                     FALDPKMKAYAVPAAGTPVTSMV"
     gene            complement(7464..>8633)
                     /locus_tag="PK614_04535"
     CDS             complement(7464..>8633)
                     /locus_tag="PK614_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB81508.1"
                     /translation="SPDTVVLMITAFASADSAVEAMKHGAYDYLTKPFQVDEVQLIIR
                     NAIERRRLSTENMLLKRELASQSSFSQIIGQSEAMQKVYDVIKKVADSKSNVLIGGES
                     GTGKELVARAIHFNSARASMPFVTVNCSAVPETLLESELFGHIKGSFTGAISNKAGLF
                     EVANGGTIFLDEIGDTTPAIQVKLLRVIQEREFRRVGGTQDVKVDVRIVAATNRDLEK
                     AVAEGAFREDLYYRLDVIPIKLPPLRMRTGDIPLLSQHFLEKFAKESGKPVPTMSQEA
                     TRVLLAHEWRGNVRELENVVERVVAFTTGSSVTDADIRGWLHKPVSNQQALPSELPED
                     GLDLEGLINTIEKDLLLKALERSQWVKKRAARLLRLNTRSFRYRLEKYEIKGGRD"
     assembly_gap    8634..8643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8644..8840)
                     /locus_tag="PK614_04540"
     CDS             complement(<8644..8840)
                     /locus_tag="PK614_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB81509.1"
                     /translation="MEKILVVDDEQGLRDVLSIMLKRAGYAVTVASDGDEAIAQVQKE
                     IFDLVITDLKMPRVGGLEVLKA"
     gene            complement(8825..>9469)
                     /locus_tag="PK614_04545"
     CDS             complement(8825..>9469)
                     /locus_tag="PK614_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81510.1"
                     /translation="RQDVGSDDTSQRLMDIAIREARRLDNIITEFLQYARPPALNLAE
                     HDLNKVLADTLDLIQHEARSRTGITIVSRMAPNQLMAQVDQDQLKQVFWNLATNAFDA
                     MSAGGTLTIATGVRRVEVGGRRSDVIEIAFQDNGEGIPKQNFDKIFLPFFTTKKEGSG
                     LGLAQVHRIVELHEGWIKVESEVGHGARFVVCLPQSAETGVRLRHEGREPWKRF"
     assembly_gap    9470..9514
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9515..>10050)
                     /locus_tag="PK614_04550"
     CDS             complement(<9515..>10050)
                     /locus_tag="PK614_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB81511.1"
                     /translation="FLVVGFLSGVLADQLRHADQSLREKEQGLNRLQVFHESIVRSIS
                     SGVFTTDEEGRITSFNPAAHEVTGYAFADVQGRLWQNVFNWHPGTPSEEAALTSLSPL
                     RFEVECSHADGSRLVLGMTVSPLHEQGTQRGLVCVFKDLTQLRYLEQEMRRREWLANL
                     GEMSAGMAHEIRNPLGAL"
BASE COUNT         2089 a         3147 c         2826 g         1933 t
ORIGIN      
        1 cttaagttca tccccctgct taaaccgccg gcttgtcgct aacccgaagc tggcaaccgg
       61 cgttccgctg ggcgtataac gcaactcggg attgcgggtc aggttcccga ccagaatgac
      121 tttattgaac cccgccaacg gtggctcctt gtggtgcgac cacgggggca cctctgggcg
      181 gagcaggctc ctgctccaac ttcacggtca gaaatttaat gatcgaatct tcaagccggt
      241 aggcgcgttc gagctcagcg atgaccgctc ctggcccctt gaagtagaag tagacatagg
      301 tgcccttgcg ctcgcgcttg atctcgtagg cgagtttctt gcggccccaa ttctcggccc
      361 gctcgagcga ggctccgttc ttggtcacgg tgcccttcat tttttcaatc aacgcagtgg
      421 tttcttcgtc gcttaaggtc ggacgaatga taaacaggga ctcgtagagt tccatgcaga
      481 atcctcctgg gcatcggccc ccattcacag atgggagcga ggtgtaggta cgccggacaa
      541 gccgggacga gctggcgaag gtagcacagc ttcaaagaat gtgtcaaaca ggtgggcggc
      601 ttttcccgcg atgccgagac cggcacgccc caatgatcga gggcccacat cctgtggcct
      661 gccgtcgcag gaagcaacag cacaggaacc atgcggtggc ttcgctatga cacccaggct
      721 cacaattttt tcttgaactg cgggggtgcg tgaggcggga gtgacggacg agggccaggc
      781 gtgccggatg gaggacgcgc tgcgccgaag ccgccatacc caccatacca gccgaacccg
      841 agcggcccgt agggaccgcc ataatacccg agtggagatc cccaatagcc gggaccatat
      901 ccaatcccat accctggccc atatccggga ccgggccagt aggaatagga ctggctgccg
      961 gcatagccgg gatcgaccag aaaatcactc tgcgccagcc gctcggcctg atgaaccgat
     1021 tgcgcacaac ccgccgggag caggatgacg cagagcgcga tgaacccagg caccaatcga
     1081 gtcatgactg catcctcaga gacctgacac ggcacgcgcc ttcattcaga cacagacact
     1141 cgcatcgaat caatatttct tctgaaactg cggcggcgca ttggccggtg gtgtgggacg
     1201 tggcgcgggt gcgctcgact gctgactgcc gatgccatag ccgcccccat accacccata
     1261 gccggaacca tagtagccga acggattcca ccagaatcca gggccgttcc aataggacgg
     1321 tgattcggga taaccgggat tcatgtgggc gcccacagat ccaagacgct ctccctggtg
     1381 aaacgattgc gcacaggccg tcaggagcaa cagcgcgctt gcgacgaaga gggaaccgat
     1441 cgatcgcgtc atggctgcac ctcctaaaaa tgataggaca ggcccacaaa gagttgatgt
     1501 gcacgatagg tcgcatcaaa gccgccgacc ggtccgaacg cccctccgaa acgaaacgta
     1561 gagtccgtat atttgtattc ggtgaacaac gccaccttcg gcgtgacgaa tgctcgaatt
     1621 ccgacgagga cattcccgcc gaccgacact tgcgtatcac tctccgtggt cgaggagcgg
     1681 ccgagtcgcg cgacaagcaa cgccggcccc ccgccgatat agggctgcca ggtcacgccg
     1741 ggatagcgca gcaccacatg ggcaccgatg ttggttacgg agagatggat ccccggcaca
     1801 tcgtcgaggt tcttgatatg cggggtactg tgcgagacgt ccagctcaat cccgaacaat
     1861 ccatggccgg gaaagacacc cagcttgcct ccgaaggcag ggcttgtttt tagattgaaa
     1921 ttcggagcat ccagaccagc cagggtcccg ctccctcgga cattccggag gggatcggca
     1981 aagtttgccc caagctgcgc ggccacatac cattcagcgc ggatcggtga gggagagcac
     2041 aatagaagca cgcagcccaa caacccggca ctcaacctgc tgcacacaaa cctagtccac
     2101 accatggtgc tgactccatg ggcgaggcct atctggatct tatcaggcaa tctgcgcggc
     2161 agactggaca actttggtgc gagcgaacga gggatgtcga atgcgtcacg gacggaacgc
     2221 tattcgctcg ggtgctggga tgttgagaga gttatttcga tctcctgcta cggctcattg
     2281 cgggcggagg aattatagag caggtacaat gcgatcccgc cggccacggc aatgcccacc
     2341 cccgccagag cacgtgtacc gaaactcagc ggccccacga caaactccgc tgcgcgaggc
     2401 aatgccaccg tcgcgataat gaccagcagt aacgcaagca gatgaatcca gaggtagatg
     2461 ggttcgccgg gttttcgcat aaatcagcgt gacgcttcac gcagtgtacg aactctggcc
     2521 ccattattcc actgtagaac aaggggcgtc aggagcgcag gcctgaggct caatcgagtg
     2581 tgaaccgctt cgtttcttcg aggtgtttgc tcaaatatgg catgaagggc gtgccgccag
     2641 tgccgacagc ggtgggattg ctggcgtgcg actggtgttg ctgaaagata taccgctcgg
     2701 cgtacccgat gtgaatgtct cgaaactgcg cgagtaaatc cacacaggta caaaaggctt
     2761 tccatagatc aggatgcgtc gctttgcgat cgcgcgcata gccgtagagc aacgggcgat
     2821 gctgctcatc cgtgcgtgct tcgagcgccg ccagaaacgt ccggtggcgc ggaggcatat
     2881 agtcacgcat ctccacgagg tactgcgtca gcggatcgtc cgcatgaggg attccaaggc
     2941 cggcatccag cgccggcaca atcgagcttt gcgagccggt ttccccacgg aattgttgag
     3001 gtcgctgcgc gtagtccgcg accccttcgt agacgactcc ctgaggcaat gacgggctgt
     3061 tcttccagcc atggatatag ggccgcacgc ggctgtaata gatgtacgga tcacaacgct
     3121 cgggcatgcg aagcagcgtc tcccgcatcg cttcctgcgc ggctgccagc gcatggagtc
     3181 cttgcagcac atcgtcgggc ttattggctc gcgcagcatt gtgagcttga accaatccca
     3241 ccaaccccgg tcctgccttc gcctcaatgt cgatatggat caggacaaac cattcttcat
     3301 ccaggccccc aagaaagttc tgcaggagca ccacgttgcc caactcgatg ggcttctgcg
     3361 gatcgaggcg ccgccagttg tgcagggcat aggaggcata ggacagcacg ggcggccggc
     3421 cgagctgttg cgcaacggca caccacggca tggccagact ggccgggatg agtgacgcgg
     3481 ggtggtcggg atcttcccag acaaaggcat gaccggcaaa ggagagggtg cgcatcgcac
     3541 aacgaaagac atcgagaccc cagtcatctg ccaccggccc catgacctct tgatggtctt
     3601 ggacgaaaga tcgaaattgt cgcgccgtca ggaattttgg aagctccgcg ccgatctgat
     3661 tcaagatcgg ctcatccggg agctgcgcca acgggtctgt cgccggaagg aacccgcggt
     3721 caggtgaaag atcgaactgc gcaagtgaga gagactggtg gctagacgtc gtcatggtcg
     3781 cttccacggg agacgcacac aattcactct accacagtgc ggccatttcc agcgcagcac
     3841 cgcactgcac ccggctcgtg tgaatccacc gggggactga aaggcagttc ttgacaagag
     3901 ctctaactcg ggcgctcgcc gccctcggct ttttcccgca cattgaattg attcatcgcc
     3961 gtggctacat cgcggttgag gagcaattcg agggcatcga cggctcgctc caggcagggg
     4021 gcatagatcg cccgctcttc aggtgacacc tgttcccgca cgtagtcggc cgtatcctgc
     4081 ctcggtgccg gacggccgac gccgatcttc agccgcacga actgcggcgt gcccaatgct
     4141 tcaatgacag atttgatgcc gttgtggccg ccgctgccgc cgtcttgcct gatccgcaca
     4201 cgacccggtt ctaaatccag atcgtcgtgc acgaccacca gggcttccgg ggtcagacca
     4261 agctcgcgaa ggagcccctt gaggggaggg ccactgagat tcatccagtc gagggtactg
     4321 gcgagttcga cgagtttgga accggcgcga cccgagcctc gctgggccgc gcctttcctg
     4381 ctgagacgta tcgaccaccg cgctgcggcc cgttcgatca cccaacaacc gatattatgc
     4441 cgtgtgtcgg catagtcggc tccgggattg cccaatccaa cgagcaggcg caacgcaact
     4501 acttcttctt ttccgcttcc ttcttcgcag gcgctgctcc ggccttgggc gcggcagccc
     4561 cagccttcgc gtctcccgcc ttgccggcct ccgcaccggc cgccccttca gccaccgcct
     4621 tgcccttcgc cgccacttca ggctccttgg ccccttcagc accaggcgcc ccggccgtga
     4681 gcaatgcttc gagcttggca tcggtcatcg gcgcggccac actcacgacc atgtgctcaa
     4741 ggtcgtccaa aaaccgcaac ccctcggtct tcggaatgtc cttcagatgg atgccctggt
     4801 tgatttggag ctgcgatgcg tcgacttcga tgaaatcggg caggacagac gggagacatt
     4861 cgatgtgcag ctgacgcaac acgtgatgca acaccccgcc ttctttcagc ccaagcggct
     4921 gaccgccggt caagtgcacc ggaactttga cgcgaatcgg cttatccatc gccacttcaa
     4981 agaaatccgc gtgcaggacg ctgcccgtaa tcggatcgac ctggaattcc cgcagcaacg
     5041 cagtacgctt ggccttgctc ttcgcgccgt tgatggcgag ggaaatgagc gcggtggatc
     5101 cggcttgaga gcgcagaatt ttcaccaact catcgggctt gactgagagc aggagacatt
     5161 ccccctgccc gtacaacacg gccgggaccc gacctgcacg acgaagttga cgcgcgggcc
     5221 ccttgccccc accctctctg ctttcaacgg ttaaatcgaa tttcatgata ctcctcctcg
     5281 accagtcgct cgacgcggac ggcctacacg aataacgaac ttactgattc atcttcatgg
     5341 atacgtcgaa tagcttcccc gaagagaggc gccaccgaca acaccttgag tttcggacac
     5401 ttctgttcct tgccacgcag gggaatcgaa tcggtcacca gcacttccgt cagaccggac
     5461 tgttggagcc gctccaatgc gggccccgat aacacgccat gtgaacaacc ggcccacacc
     5521 tcacgcgcgc ctttatccag gcaggcctga gcgccctgga cgatcgttcc tgcggtatcg
     5581 atcatatcgt cgaggagcaa cgcgctcttg ccctgcacgt caccgatgat attcatgacc
     5641 tgcgcctggt tgggtccctc gcgccgcttg tcgataatgg ccagattggc ctgcaagcgc
     5701 ttcgcgaagg ctctggcgcg ctccacgccg ccggcatccg gcgacacaac aaccagatcc
     5761 gacaccttcc gtttcgtgat gtagtccatc agcaccggca aagcgtagag gttatcgact
     5821 ggaatattga aaaacccctg gatctggctc gcatggagat ccatggtcag cacacggtcc
     5881 gcaccggccg tggtaatgat gtcggcgacc agtttcgctg agatcggcac acgcggctga
     5941 tccttgcggt cctgccgggc atacccgaaa tagggaatca ccgccgtgat acggttggcc
     6001 gacgagcgct ttaaggcatc gatcatgatg agcatttcca tgatcgaatc gttgaccggg
     6061 gaacagcagg attgggtgac gaagacgtcg gcgccacgca cgttctcttc cacgcgaatc
     6121 cggatttctc cgtcgctgaa ggaagacacc gtcgccgcgc caaggggcag gccgagatac
     6181 gagcaaatcg cccgcgcaag cgcaggattg gcgctgcccg aaaagagctt gagttccctg
     6241 ttcatccgac cccttcgatg ccgtacgagg cgatgcctat gctgtaacgg tgcttgtcga
     6301 gttcgtaaaa aacggtgggc acggacagga cccgctgtga gtcccgctga cctcgcgcac
     6361 ttgtcaggac aagaggacgc ctaccgaagc acagcacaat agcgaaaacg tctggaagtt
     6421 gtcaaccaat ctgcggcagg cgaagcgtcc accgtgcgat gtgggtgggg cgctcagcca
     6481 ccttttgata gaccgtggcc ttgccccctc tggccttgcc cttgaggact acggtctcaa
     6541 gactcagacc atgctcgtga ccggagtacc cgccgccggt acggcatagg ccttcatctt
     6601 cgggtcaagg gcaaacacag aggcagcccg ctccgcatcg gcctgcccgg gaaatacccc
     6661 gaacatcgtg gcaccgcttc cggacaacag ggccacttca gctccctgac tcagcaattg
     6721 gctcttaatc tgggccaata ccggatgctg caaaaaaacc ggcgcctcaa aatcattctc
     6781 taccagggga atgatctcgg accaatccac tgccgcacgg ctgcccagtt gctgcaatgc
     6841 cggagagagc ggacgcaccc ccgccctggt ggcagacagc tgctgatagg cccactttgt
     6901 ttcgacagga aagccgggat tcaccagcac gatccagcga gtgcccggca tctgaattgg
     6961 gcgcacctgt tcccctcgac cggtgacaca ggcggccggc cccataaaaa agaacggcac
     7021 gtcacttccc agctgttgcc ccacctcagc catctgctca atcggccagt tcaatcccaa
     7081 caggcgggtg agaccgagga tggtcgccgc cgcgtcactg ctgccgcccc ccaagccggc
     7141 ccccagcgga atgcgtttcg tgagggtgat cgacagatcg acggctcggt cgattcgcgc
     7201 caacacgagt tgcgcggctc gatagacaag attactttga tcggccgcca gcgcgacgtg
     7261 gtcgcagcgc aaagcgatac gggaggtcgt gccgggctgg acggtaagag tcagctcgtc
     7321 ttcgagtccg accgtgtgca tcagcgacca gacggcgtga aagccgtcag gacgccggtc
     7381 gagcacgcgg aggatcagat tcactttcgc aggggtcaga acacgcaggc tggtagagtt
     7441 cactcgcaga ttactgggcg gcgctaatcc cggcctcctt tgatttcgta tttttcgaga
     7501 cggtaccgga acgaccgggt attcaaccgc aacaaccgcg cggctcgctt cttgacccac
     7561 tgactccgtt cgagcgcttt caacagtaga tccttctcga tcgtgttaat gagcccttcc
     7621 aggtccaggc catcctcagg caactcagag ggcagggcct gctggttgct caccggcttg
     7681 tggagccatc cgcgaatatc cgcatccgtc accgacgatc ccgttgtgaa ggccacgaca
     7741 cgttcgacga cgttctccaa ttcccgaacg ttgcctcgcc actcatgcgc caacagcacg
     7801 cgcgtggcct cctggctcat cgtcggcact ggtttaccac tttccttggc aaatttctcc
     7861 aggaaatgtt ggcttaataa cggaatgtcg ccggtccgca tgcgcagcgg gggaagcttg
     7921 atcgggatca catccagccg gtagtacaga tcttcccgga aggcgccctc tgcgacggct
     7981 ttctccaaat cacggttcgt ggccgcgacg atgcggacat ccaccttcac atcctgggtc
     8041 ccgcccaccc gccggaactc acgctcctga atcacccgca gcagcttcac ctggatggcc
     8101 ggtgtcgtat cgccgatctc atcgaggaag atcgtcccgc cgttcgccac ctcgaacaga
     8161 ccggctttat tggagatcgc cccggtgaac gaacccttga tgtggccgaa tagttcactt
     8221 tcaagcagag tttccggcac cgcgctgcag ttcaccgtca cgaacggcat ggaggcgcgc
     8281 gcactgttga agtggatcgc ccgagcgacc aactctttgc cggtcccgct ttccccgccg
     8341 atcaacacat tgcttttcga atcggccacc ttcttgatga cgtcgtagac cttctgcatc
     8401 gcctcgctct gaccgatgat ctgagagaac gacgactggc tggccagctc ccgcttcagg
     8461 agcatattct ccgtcgataa ccggcgccgc tcgatcgcgt tgcggataat cagttgtact
     8521 tcatcgacct ggaacggctt ggtgagatag tcgtaggcgc cgtgcttcat cgcctcgacc
     8581 gccgagtcgg cggacgcaaa ggccgtgatc atcagcacca cggtgtccgg agannnnnnn
     8641 nnngccttca gtacctccag cccgcccact ctgggcatct tgaggtcggt gatgacgaga
     8701 tcgaaaattt ccttctgcac ttgcgcgatc gcttcgtccc cgtccgaggc gacggtcacg
     8761 gcatatccgg cgcgcttgag cataatgctc aacacatcgc gcaagccctg ctcatcatcg
     8821 acgactaaaa tcttttccac ggttcccttc cttcatgccg aagccggaca cccgtctctg
     8881 cggactgcgg gagacacacg acgaagcgtg ccccgtgccc gacctcgctc tcgaccttga
     8941 tccacccctc gtgcaactcc acaatgcgat gaacctgggc cagccccaac ccggagcctt
     9001 cttttttcgt cgtaaaaaac ggcaaaaaga tcttgtcgaa attctgtttg ggaatgcctt
     9061 ccccgttatc ctggaatgcg atttcgatga cgtcggatcg gcggccaccg acctccaccc
     9121 gccgcactcc ggtggcgatc gtcagcgtcc ctccggcgga catcgcatcg aaggcgttgg
     9181 tggctaaatt ccagaagacc tgcttcagct ggtcttgatc gacctgcgcc atgagttggt
     9241 tcggtgccat gcgggagacg atggtgatgc cggttcgaga ccgcgcttca tgttgaatca
     9301 gatccaatgt atcggcaagg actttattca aatcgtgctc ggccaaattc aacgcggggg
     9361 ggcgggcata ttgcagaaac tcggtgatga tattgtcgag ccgcctggct tcccgaatcg
     9421 cgatatccat caaacgctga ctggtgtcgt ctgacccgac atcctgccgn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaagcgc gccgagcgga ttgcggattt
     9541 catgcgccat gcccgccgac atttcaccaa gattggccag ccattcccgc cggcgcatct
     9601 cctgctccaa gtagcggagt tgcgttagat ccttgaatac acagaccagg ccgcgctgcg
     9661 tgccctgttc atgtagcgga gagaccgtca ttccaagtac caagcggctg ccgtccgcat
     9721 gcgaacattc cacttcaaat cgcaagggag agagggacgt caaggcggct tcctcggagg
     9781 gggtaccggg gtgccaattg aagacattct gccagaggcg cccctgcacg tcagcaaagg
     9841 catagccggt gacttcatgc gccgcggggt tgaaggacgt gatccgcccc tcctcatcgg
     9901 tcgtgaacac cccgctgctg atacttcgca cgatactctc gtgaaatacc tgcagccggt
     9961 tgagcccctg ttccttctcg cgcaacgact gatccgcatg acgaagttgg tccgccaaca
    10021 ctccgcttaa aaatcccacc accagaaatg
//
LOCUS       DAORQN010000046        10038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_366_length_10038_cov_10.305519, whole genome shotgun sequence.
ACCESSION   DAORQN010000046 DAORQN010000000
VERSION     DAORQN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_366_length_10038_cov_10.305519"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PK614_04555"
     CDS             <1..1228
                     /locus_tag="PK614_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB81512.1"
                     /translation="FSFTAKFAVHVQPAPAVPPAQWAQLASLRTLIVDDNATNRLIVR
                     ETLAGWGIPAAEVPGGEEALFELRRASKAGVPYRLVILDVRMPKLSGWQVAETIARTP
                     GLAGVSIIMLTSERRAGDQARARECGVVRYLTKPFRRSDLFNGMMAVIGKVALAEGNA
                     LLSQPPDSTGDMPGLKVLLAEDFVDNRRMMEFYFKSTPHRVETAANGQLAVEMFQRGS
                     YDLVLMDIQMPVLDGYAATRAIRSWEQAQGRSPVPILALTANALQSEVQRSLEAGCTT
                     HLTKPIRKARLLEAIQLYFQTSVPVVPSSSDASADTVVLQVSGEFESLMPGFLEHRRQ
                     ELIQIADALDRGDFETIREIGHGLKGAGGTYGLEAISAYGRALETAAGQKDGVCVREA
                     LHRLTRFLERLQLVYV"
     gene            1243..2637
                     /locus_tag="PK614_04560"
     CDS             1243..2637
                     /locus_tag="PK614_04560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB81513.1"
                     /translation="MRVLLVEDHIDIRRLFEQVIEARGHDVTACADGESAWEAYSRRP
                     YELVLLDWELRGGGMDGLQVCRTIRSSPGGDRCVIVMITGHDSPESLRTALQAGVNDY
                     LVKPVGVEFLKLRLTIAEQWVESVRRRFAAEDQAQALQSQLADHGKFHDLIGRSPAMV
                     VLYEEIQKIAAVDATVLIEGETGTGKELVARAIHLSSRRASHTFLAVNCAGFTDSILG
                     SQLFGHKKGAFTGAIDNQEGVFEAAQGGTIFLDEIGDISPAVQTSLLRVLQEREVTRL
                     GESKPRKVDVRVVVATHHNLVEDVEKGTFRRDLLYRIKVARLQLAPLRERPTDIPLLV
                     HAFLGQFRSVMEKPVLQVSPEALQVLISHSWPGNVRELKSAVESALIHCKGTVVRVED
                     LPPEVRNPEPAATYVPLQRQDERTRMVGALQQTGGNRSEAARLLGMSRRTFYRRLAEY
                     DVSAPGDGGDAPLQ"
     gene            3020..3286
                     /locus_tag="PK614_04565"
     CDS             3020..3286
                     /locus_tag="PK614_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81514.1"
                     /translation="MDVAGFLLSYDADQEVLVTIQPSSLGPFDQPDVKEQIDGSWIDM
                     SRVPHRTWTDRTRAVGAVLTYLQGLSPISSGFPLATREGMKGQN"
     gene            3303..3623
                     /locus_tag="PK614_04570"
     CDS             3303..3623
                     /locus_tag="PK614_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81515.1"
                     /translation="MWSEQQNVVQVEREANVQGSTPGIAQALGGGLAAKRRPEDLTPR
                     EQEILQLVWAGLTNRTIAERLHISIKTAEAHRANMMKKLRVSNIAQLLKTALEEGLLA
                     THAG"
     gene            3773..4165
                     /locus_tag="PK614_04575"
     CDS             3773..4165
                     /locus_tag="PK614_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB81516.1"
                     /translation="MVEQRIEEITRANLAFYAAFESLDMLQMDKVWAHLEYVTCIHPG
                     WSLRSDWPAVRDSWVLIFNNTFSMKFELSDVQVQVAGDLGWVICTEHLTSRQDDQPVE
                     TRVLATNLFERIGDEWLMIHHHGSPVMG"
     gene            4188..4595
                     /locus_tag="PK614_04580"
     CDS             4188..4595
                     /locus_tag="PK614_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81517.1"
                     /translation="MSGEDRRSLVVGWVALGLFFLSGCTTVAQVTTWSDESCRQKVKD
                     QLQSILTDEGEQGDIANRLAVNTTVVLATGSLGPRPFGVASPSGTDYSFFVQRKGEGC
                     LLRLFGHQKGFTRYTNNLTYISTRSLDGCACAE"
     gene            5012..5257
                     /locus_tag="PK614_04585"
     CDS             5012..5257
                     /locus_tag="PK614_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81518.1"
                     /translation="MMVKGFLLKDREEQEYLLAIQPATHGMFERLIVRQKVDGSWVEL
                     NGNAQGSWQPRTQAITAVLGYLQRLWDTSKPALARGN"
     gene            5317..5673
                     /locus_tag="PK614_04590"
     CDS             5317..5673
                     /locus_tag="PK614_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB81519.1"
                     /translation="MLAEKKDSRVGNQEVAEDGVFTTHRPSSDALAPAAVKRRPEDLT
                     PRELEILRLIWDGHTNRSLAEQLNISMKTADTHRSNMMKKLRACNTAQLLKAALEGNL
                     LETGSGLRRPGRGGQR"
     gene            complement(5757..5993)
                     /locus_tag="PK614_04595"
     CDS             complement(5757..5993)
                     /locus_tag="PK614_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81520.1"
                     /translation="MSGQSCLIDGCQARVYAPAGTHAICKDHFLGFLTWRRRRGPQMF
                     RKYAGMTMDERDVIAGEWLQTLGTKDLPHPIPGL"
     gene            6246..6500
                     /locus_tag="PK614_04600"
     CDS             6246..6500
                     /locus_tag="PK614_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81521.1"
                     /translation="MAMQVISVVGALMVLVAYGLIQAGTWRELDAGYLALNILGSLLL
                     GIVAIEDRRVGFVLLEFAWAGIALVGVARAIRARRAARSL"
     gene            6555..7037
                     /locus_tag="PK614_04605"
     CDS             6555..7037
                     /locus_tag="PK614_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81522.1"
                     /translation="MAGLLSADEIFEKAKDAAVGATTADEKALQIDYEALQLKFQAAL
                     GDRKVARCHINKFLPEGYEDQGRFNLVLLTAGNVIFDIVIGDSYFRYDVVSVGQLDKV
                     QVIDAMWDNREKRREEPFLSLRLMHAEETHLLLALDDDERKSLLGFASAVSAVRNPEK
                     "
     gene            complement(7215..8306)
                     /locus_tag="PK614_04610"
     CDS             complement(7215..8306)
                     /locus_tag="PK614_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HRB81523.1"
                     /translation="MKQSRLHDQHQQLGAIFEDTAGWSMPTHYGDPAAEYAAVRGAVG
                     LSDLSHRGKIRVTGDDRIKWLQSIISNDILPLQPGQGRYSSFLTHKGKMLGYFRVYVQ
                     ADAVWVEDVGEVGDATFQALRKFLLYGTKAKMENCGESWGLLLVSGPKSAEAVAAAFG
                     IEVRALQLLHTLPATIGGQQGLILRTEETGEQDFEVLLPADAVPAAWNQLMTSGAPFG
                     IKPVGAQARELFRIEAGLPKAGPDLNEEIVPPEANLEGKAFSLSKGCYPGQEVVARMD
                     TYGNVRRHLVGLIIQDKTVPPAGSKLFSGEREVGWVSSAVFSPQRNAALAFGFPLRDF
                     SAPDTTLTVEVAGTRHQATVHALPFQKHS"
     gene            complement(8327..8740)
                     /locus_tag="PK614_04615"
     CDS             complement(8327..8740)
                     /locus_tag="PK614_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRB81524.1"
                     /translation="MNTTILVAVTDIFFYTKVRDALRPQGYTLERIRSQDEVAEKTAS
                     ASPAALILNMNDLAIDAFKALETLQADPALRSLPILAFANHEEVDTWRRAKELGVTKI
                     VSRNEFSTRTRELVEDIIANSSQQSPVRSELNAEG"
     gene            complement(8847..9275)
                     /locus_tag="PK614_04620"
     CDS             complement(8847..9275)
                     /locus_tag="PK614_04620"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81525.1"
                     /translation="MDVQDFLIQGDGSEEDKREAWQLFQQAYEQQMKGQLEEAVSLYK
                     LSLATHQTAEAYTFLGWTYSFMGKLDEAIDECHKAIACDPHFGNPYNDIGAYLIEKGD
                     LEEAIPWFQKAMQARRYESPAFPPESWPCLRTPGQVERGD"
     gene            complement(9355..>10038)
                     /locus_tag="PK614_04625"
     CDS             complement(9355..>10038)
                     /locus_tag="PK614_04625"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HRB81526.1"
                     /translation="GLICAHDLGRMGIKTLIVEQSLALGGGFWSGGYLMNKATICAPA
                     HKILKEVGVPCKQIKECPGMYMVDPPHATGALIAAAYNAGAKIMNLTRVVDLILRREG
                     VLEGVVVNSTTAEMAGHDIIHVDPIALESKIVVDATGHDAVVVNLLHKRGLYQQVPGN
                     GAMWVSRSEEEVMDRTGEVSPNCFVIGLAVAAVFGTPRMGPAFGSMLLSGRYGAELIQ
                     KKLKQGKSI"
BASE COUNT         1966 a         2775 c         3153 g         2144 t
ORIGIN      
        1 gttcagcttc acggccaagt ttgctgtgca tgttcagccg gcgcccgcgg tcccgccggc
       61 gcagtgggcg caattggcga gcttgcggac cttgatcgtg gacgacaatg cgaccaaccg
      121 cctgattgtg cgggaaacgc tggcgggatg gggcattccc gcggccgagg tccctggtgg
      181 cgaggaggcc ctgtttgagc tgcgtcgcgc gtcgaaggcc ggagtgccgt atcggttggt
      241 gatcttggat gtgcgcatgc cgaaactcag cgggtggcag gtcgcggaga cgattgcacg
      301 tactccgggc ctggcaggag tatccattat catgttgacc tcggagcggc gcgccggtga
      361 ccaggcgcga gcccgtgagt gcggggtggt gcgctatctc acgaaaccgt ttcgacgatc
      421 ggatttgttc aacggtatga tggcggtgat cggcaaggtg gcgttggctg agggcaatgc
      481 gctgctgtct cagccgccgg attcgaccgg ggacatgccg gggctgaagg tcttgttggc
      541 cgaagacttt gtcgacaacc gccgcatgat ggagttctat tttaaatcga cgcctcatcg
      601 ggttgagacc gcggccaacg gccaacttgc cgtcgagatg ttccagcggg gctcgtacga
      661 tctggttctg atggatattc agatgcccgt gctggatggg tatgccgcga cgagggcgat
      721 tcggtcgtgg gagcaggcgc agggccggag tccggtaccg attctggcgc tgacggccaa
      781 tgcgttgcag agtgaagtcc agcggagtct ggaggcgggg tgtacgacgc atttgacgaa
      841 acctatccga aaggcccgcc tgctggaagc catacaactg tatttccaaa cttccgttcc
      901 ggtcgtgccg tcgtcgtcgg atgcgtcagc cgacaccgtc gtgttgcagg tgagcggaga
      961 gtttgaatct ctgatgccgg ggttcctaga gcatcgacgg caggaactca tccagattgc
     1021 ggacgcattg gatcgaggag attttgagac gattcgcgag atcgggcatg gccttaaggg
     1081 ggccggcggg acctatggcc tcgaagcaat cagcgcctat ggacgagcct tggagacggc
     1141 ggcgggtcag aaggacggcg tgtgcgttcg cgaggcgctc cacaggctca cccgttttct
     1201 ggagcggctg caactggtct atgtgtaagg agtaagcaag ccatgcgtgt cttgctggtg
     1261 gaagatcata tcgatatccg gcgcctgttc gaacaggtca ttgaggcgcg gggacatgac
     1321 gtcacggcct gcgcggacgg cgagtcggcc tgggaggcct atagtcggcg accctatgaa
     1381 ctcgtgctgc tcgattggga gttgcggggg ggcggaatgg acggcctgca ggtgtgccgg
     1441 accatccgat cgagtcccgg cggcgaccgc tgtgtcattg tcatgatcac cgggcatgac
     1501 tccccggaat ccttgcgtac ggcgctccag gcaggggtca acgactatct ggtgaagccc
     1561 gtcggtgtgg agttcttgaa gctgcggctg acgatcgcgg agcagtgggt ggagagtgtg
     1621 cgacggcgct tcgcggcgga agatcaagcg caggccttgc agtcgcagtt ggccgatcac
     1681 ggcaagttcc atgacttgat cggccggagt cccgcgatgg tggtgctcta cgaagagatc
     1741 cagaagatcg ccgctgtcga tgcgaccgtg ttgatcgaag gcgaaaccgg tacgggaaaa
     1801 gaattggtgg cccgcgcaat tcatctctcc agccgccgcg cgtcccacac ttttctggcc
     1861 gtcaattgcg ccgggttcac cgactccatt ctgggcagcc agctcttcgg tcataagaag
     1921 ggggccttca caggcgcgat cgacaatcag gagggtgtgt tcgaggccgc gcaggggggc
     1981 accatatttc tggatgagat cggcgatatt tctcccgccg tgcagacgag cctcctgcgt
     2041 gtgctgcaag aacgtgaagt gacccggttg ggagaatcga agccgcgcaa ggtggatgtg
     2101 cgggtggtgg tggcgacaca ccataacctc gtcgaagatg tcgaaaaagg cacgttcaga
     2161 cgggacctgc tgtatcgcat caaggtggcg cgactccaac tcgccccact tcgtgagcgg
     2221 cccaccgaca ttccgttact ggtccatgcc tttctcggac agttccggtc cgtgatggag
     2281 aagcctgtgt tacaggtgag tccggaagcc ttgcaggtcc tcatcagcca ttcgtggccc
     2341 ggcaatgtgc gagaactcaa gagtgcggtg gagtcggccc tgattcattg caaggggacc
     2401 gtcgtacggg tggaggatct tccgccggag gtccggaatc cggagccggc ggcgacctat
     2461 gtgccgcttc agcgccagga cgaacggact cgcatggtcg gggcgctgca gcaaacgggc
     2521 ggcaaccgct cggaagccgc acggttgtta gggatgagcc gacgaacttt ctatcgccgc
     2581 ctggctgaat acgatgtatc cgcaccgggt gatgggggag atgcacccct tcagtgaatc
     2641 cgcctcgatt gcgatctaaa tgcgaggacc tgtacctccg cgtcagatag atctttcctc
     2701 tgaatccacc atttcctact ttcttcgcct gacgagccat ttcgcgcgta tcagattcag
     2761 cgtagccgca cccgcgtgtg caggtgctga aactgtgcaa cgaggcaaca tgacacaggt
     2821 ctggcacaac gcattgcgcc atgtccttga cgcgcgaaat ggatggattt tctatcaatc
     2881 agcgcaagtg cctagtattg cttgatgctt cacccgccgt ctttgatggc acgatccttg
     2941 atataagaac aggcatggag cgagttacca ccggccataa cgggtcgaac gacggggaga
     3001 ggccaaagag gggacgcata tggatgtcgc aggattttta ctctcatacg atgcggatca
     3061 ggaagtgttg gtgacgattc agccctcgtc cctcggaccc ttcgatcagc ctgatgtaaa
     3121 agaacagatt gacggatctt ggatcgatat gtcgcgagtg ccgcaccgaa cctggacgga
     3181 tcgaacgagg gcagtcggag cggtgctgac ctatctgcaa gggttgtcgc cgatctcatc
     3241 gggatttccg ctcgcgactc gtgaaggaat gaagggccag aactagaacc aaggagagca
     3301 ccatgtggag tgaacagcag aatgttgtgc aggttgaacg tgaggcgaat gtgcagggct
     3361 cgacaccggg gatcgcgcag gcgttgggcg gaggcctggc ggccaaacgg cgtcctgaag
     3421 acttgactcc tcgcgaacag gaaatcttgc agttggtttg ggccggcctg acgaatcgta
     3481 cgatcgcgga gcggctgcat atcagcatca agaccgcgga ggctcaccgc gccaatatga
     3541 tgaaaaaatt gcgtgtgtcc aacattgcgc aactcttgaa gaccgcgctg gaagaggggc
     3601 tgctcgccac ccatgcgggc tgatggggtt cgcggcggcc tgcgttctcg ggggaagtcc
     3661 cactcccggc ggcgcagcac ttccccgcga atccgccccc tcacagcata aatgaggctc
     3721 gcgccggcga gggtgtttgc agtagaatgc cgcaccgttg caggagcgag ccgtggtcga
     3781 acagcgtatc gaagaaatca cccgcgccaa tctcgccttt tatgccgcat ttgaaagcct
     3841 ggacatgctc cagatggaca aagtatgggc gcatctcgaa tacgtcacct gcattcatcc
     3901 cgggtggagc ctccgcagcg actggccggc ggtgcgcgat tcctgggtgc tgatcttcaa
     3961 caacaccttc tcgatgaaat ttgagctgtc ggacgtgcag gttcaggtcg caggggacct
     4021 ggggtgggtc atctgcaccg aacatctcac cagccgtcaa gacgaccagc cggtcgaaac
     4081 gcgtgtcctc gcgaccaatc tctttgagcg tatcggcgac gagtggttga tgatccatca
     4141 ccatggctcg ccggtgatgg gctagcccca tcgctgggga ggtccggatg tccggcgaag
     4201 atcgaaggtc tctcgttgtt gggtgggtgg cgttgggact cttcttcctc tccggctgta
     4261 cgaccgtcgc tcaggtgacg acgtggtcgg atgagtcctg ccggcagaag gtcaaagacc
     4321 agcttcaatc tatcctcacg gatgagggtg aacaagggga tatcgcgaat cgcctggccg
     4381 tgaataccac cgtggtgttg gcgaccggtt ctttgggccc ccgccccttc ggtgtcgctt
     4441 cgccctccgg caccgactat agtttcttcg tgcagcgtaa aggtgagggg tgtctcctgc
     4501 gattgttcgg ccaccagaag gggtttaccc ggtacaccaa taacctgacc tatatttcca
     4561 cgcgttccct tgacggttgt gcttgcgctg aatgaccgtc gcctgatggt tgcgcttcca
     4621 gtttccctgc cgttctcttt ggttctgtaa accgtcgccc cgtcggtcac gcacaattcc
     4681 ctctcgctgt ctggttcctc aagatcccca gtatccgttc ctatacagca gatgcacggg
     4741 gctaatctgt cgcctgaaag tgtctcgcga aactgtctca cgcttgaatt ttggcacact
     4801 cttgtttctt gtggcacaac ttgtggcgtt tgggataaat ccagaattct tgctcaacat
     4861 attgttttat ttggaataat atctttccgt cgtcatttgg cacagccctt gatgtagcgg
     4921 ggttatgtct cgtagggcaa agaccttggg gactttgatg gagacggcat tcgagggtcc
     4981 tgagtgtgca gaaaaaaagg gaggagtgat catgatggtg aaggggttct tactcaaaga
     5041 cagggaagag caggagtatc tgctggccat tcaaccggcc acccacggca tgttcgagcg
     5101 gttgattgtc cgccaaaagg tcgatgggtc ttgggtcgag ctcaatggga atgcacaggg
     5161 ttcctggcag ccgcggacac aagccattac tgctgtgttg gggtacctgc aacgtctctg
     5221 ggacacgtcc aagcctgcct tggcgcgggg gaactaaaaa aagcggcatt ctccgcgaag
     5281 tgaagcatgc ggaaagggtg acgaaggagg gacttgatgt tggctgaaaa aaaagacagc
     5341 cgggtaggga atcaagaggt ggcggaggac ggggtcttca caacacatcg tccatcgtct
     5401 gacgctcttg caccggccgc cgtgaagcga aggccggagg atctcacgcc gcgtgagttg
     5461 gaaattcttc gcctgatctg ggatggacat accaatcgaa gtctcgcaga gcagctgaat
     5521 atcagcatga agacggccga tacccatcgc tcgaacatga tgaagaagtt gcgcgcgtgt
     5581 aatactgccc aattacttaa agcggcgctg gagggaaatc tgctagagac gggctcgggc
     5641 ctgaggcgtc ccggtcgggg aggccaacgc tgaggaccga cagccaggag accggtttga
     5701 attgcctgac cttccgtccg gtgcaggaca ggaaggtgca ggtgaagcgg agagacttac
     5761 aagccgggaa tggggtgggg caggtctttg gtgccgaggg tctggagcca ctcacccgcg
     5821 atcacatcgc gttcgtccat cgtcatgccg gcatatttac gaaacatctg tgggccccgt
     5881 cgccgcctcc acgtgaggaa gccgagaaag tgatccttgc agatggcatg ggtgcctgcc
     5941 ggcgcataca ctctcgcctg acatccgtcg attaaacagc tctgtccgct catcggtatc
     6001 ccccctgatt gtcgcaaagt atgccggaga tcgacctgga aatgcaatgt ggtggctcgg
     6061 cgagcgcagc ctgccagaca ggccgacgca ccgcgctgga gtgatgctca accacatcgg
     6121 caccagccca tcagcgtctc cttgcccgct tcttcatggg gagtttccgc ttgcaatcac
     6181 atccagcagc caggtaccgt accccgttct cacgcggcgt ccggcaagga gtgcccgagc
     6241 ataccatggc gatgcaggtc atatcggtgg tcggagctct gatggtgctc gtggcctatg
     6301 gcctcattca ggccggcacc tggcgtgagt tggacgcggg gtatctggcg ctgaacatcc
     6361 tgggctcgtt gctgctggga attgtagcca tcgaagaccg acgggtgggt tttgttctgc
     6421 tggaattcgc gtgggccggc attgcgctgg tcggggtcgc ccgggcgatc cgcgccaggc
     6481 gggctgcgcg tagcctctaa atcgcccaca cacaataaac atcgggcagt cttgtaacga
     6541 aggagtaggg aactatggca gggttgttgt cagcagacga gatctttgaa aaggcgaagg
     6601 acgcggcggt cggggcgacc actgccgatg aaaaggcgct ccaaatcgat tacgaagcat
     6661 tgcaactcaa gttccaggca gccttgggcg atcgcaaagt ggcgcggtgc catatcaata
     6721 agttcctgcc cgaagggtac gaagatcagg ggcggttcaa tctagtgctg ctcacggccg
     6781 gcaacgtcat tttcgacatc gtcatcgggg attcctattt ccgctacgat gtggtgtccg
     6841 tcggtcagct ggataaggtg caggtcatcg atgccatgtg ggataaccgc gaaaagcgcc
     6901 gggaagaacc cttcttgagc ctccggctga tgcacgccga agagacgcat ctgttgttgg
     6961 cattggatga cgacgagcgg aagagcctgc tcggattcgc gtcagccgtg tcggccgtgc
     7021 ggaatcctga aaagtagaac acctctgcca ctcgaaaggg tggtcatcgg ttcgtgtgat
     7081 tgaagccccg cagggtgttc aaaacggccg ttcaggaggg ccgcagctgg tgaagcactg
     7141 atgcgtgccc tcaggggtac gttgaggcgc tgaatgccgc gagaacgagg ccggcggacg
     7201 ttttcagcat cctgctacga gtgcttctga aacggtaggg catggacggt agcctgatgg
     7261 cgcgtccctg ccacttcgac cgtcagtgtg gtgtcaggtg ccgaaaagtc gcgcaatgga
     7321 aagccgaatg ccagtgcggc attgcgttgc ggagaaaaga cggcgctgct gacccatccc
     7381 acctcccgct ctccgctgaa taacttcgag cccgcgggcg gaaccgtttt gtcctggatg
     7441 atcaatccga ccagatgccg gcgcacgttc ccataggtat ccatcctggc cacgacttcc
     7501 tgtcccgggt aacaaccctt tgagaggctg aacgccttgc cttccaggtt ggcttccggc
     7561 gggacaatct cttcgttgag atccggaccg gccttgggca gaccggcttc gatgcgaaac
     7621 agttcgcgcg cctgtgcgcc gacgggcttg atgccgaacg gtgcgcctga tgtcatgagc
     7681 tggttccagg cggcggggac cgcatcggcc ggtagcaaga cttcaaagtc ctgttcgccg
     7741 gtttcttcgg tacgcaggat cagtccctgc tgcccgccga tcgtggcggg gagggtgtgt
     7801 aagagctgaa gggcgcggac ctcgataccg aatgccgcgg cgacggcctc tgctgatttc
     7861 ggtccgctca ccagtaacaa gccccagctt tctccgcaat tctccatctt agccttggtg
     7921 ccgtagagca ggaactttcg caacgcctga aatgtggcat cacccacctc gcccacatct
     7981 tcgacccaca cggcatcggc ttgcacatat acccggaagt agccgagcat tttcccctta
     8041 tgggtcagga agctggagta gcgcccctgg ccgggttgga gcgggaggat gtcgttgctg
     8101 atgatgctct ggagccactt gatgcgatcg tcgccggtca cccggatttt tccgcggtgc
     8161 gacaggtcgg acagcccgac ggcgccacgg acggcggcat attccgcggc gggatcgccg
     8221 taatgggtcg gcatcgacca tcctgcggtg tcctcgaaaa tggcgcccag ttgttggtgc
     8281 tgatcgtgga gtcgggattg tttcattgga ttcttactct ctgttctcag ccttcagcgt
     8341 tcagctctga gcggaccggt gattgctgac tgctgttggc tatgatgtct tcaaccagct
     8401 ctctggttcg tgtggagaac tcatttctgg agacaatttt cgtcacgccc agttccttgg
     8461 cgcggcgcca ggtgtccact tcttcgtgat tggcaaaagc caggatggga agcgaacgca
     8521 acgcggggtc ggcctggagg gtttccaacg ccttgaaggc atcgatggcc aggtcgttca
     8581 tgttgagaat cagggcggca ggcgaagctg aggcggtttt ttcagccacc tcatcctggc
     8641 tgcggatccg ctccagcgtg tagccctgcg gccgcaaggc atcgcgtacc ttggtgtaaa
     8701 agaaaatgtc agtcacggcc actaaaatcg tggtgttcat ccggttcctg tcctatggtc
     8761 cctaattcag tgagctgatg agccgaccca gagccttctt ggccaaggcg tagtccgggt
     8821 tcagggcgag cgcctgtttg tagcagtcaa tcgcctcgct ccacttgccc tggcgttcgt
     8881 agacacggcc aagattcagg tgggaaagcc gggctctcat accgtctggc ctgcatggct
     8941 ttttgaaacc agggaatcgc ctcctccaga tcaccctttt cgatcaggta ggctccgatg
     9001 tcgttgtagg gattcccgaa atgcggatcg caggcgatgg ccttgtggca ttcgtcgatc
     9061 gcctcgtcca gcttacccat gaagctgtag gtccatccga ggaacgtata ggcttcggcg
     9121 gtctgatgcg tcgcgagcga cagtttgtag agactgaccg cttcttccag ctgccccttc
     9181 atctgctgct cgtaggcttg ctgaaagagt tgccaggctt cgcgcttgtc ttcttcgctg
     9241 ccgtcgcctt gtataagaaa gtcctgaaca tccatgtgtg atcacaagag ccaatggctt
     9301 atggagtata gcagttcgat ggtccgttcc ctgctaccag ccgttcgcta tcagctatat
     9361 gctcttgcct tgtttcagtt tcttctgaat taactccgcg ccatagcggc cggagagcag
     9421 catcgatccg aaggccgggc ccattcgtgg tgtgccgaac acggccgcga cagccaaccc
     9481 gatcacaaag caattgggag agacttcccc ggtccggtcc atcacttctt cttccgaccg
     9541 cgacacccac atcgcgccgt tgcccggcac ctgctggtag agcccccgct tatgcaggag
     9601 attgaccacc acggcatcat gtccggtggc atcgaccacg attttactct ccaaggcgat
     9661 cggatcgaca tggataatat cgtggcccgc catttcggcg gtggtgctgt tgacgacgac
     9721 gccttcaagc acgccttcgc ggcgcaggat cagatcgacg acccgcgtca ggttcatgat
     9781 cttcgccccg gcgttgtagg cggcggcaat cagcgctccg gtggcatgcg gaggatcgac
     9841 catgtacatg ccggggcatt ccttgatctg cttgcaaggc acgcccactt ctttgagaat
     9901 cttatgcgcg ggggcgcaga tggtggcttt gttcatcaaa tacccgccgg accagaagcc
     9961 gccgccgagg gccaaactct gttccacgat gagggtcttg atgcccatcc ggccgaggtc
    10021 gtgggcgcag atgaggcc
//
LOCUS       DAORQN010000047         9917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_371_length_9917_cov_23.543196, whole genome shotgun sequence.
ACCESSION   DAORQN010000047 DAORQN010000000
VERSION     DAORQN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_371_length_9917_cov_23.543196"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /gene="ispH"
                     /locus_tag="PK614_04630"
                     /gene_synonym="lytB"
     CDS             complement(<1..402)
                     /gene="ispH"
                     /locus_tag="PK614_04630"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRB81527.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEAQSRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYELILIGHAGHPEVIGTLGQI"
     gene            576..1292
                     /locus_tag="PK614_04635"
     CDS             576..1292
                     /locus_tag="PK614_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81528.1"
                     /translation="MPPPTTTHKKILIVEDEKDILQLVKLYLEKEGFRTVSAMTGTEG
                     LRQVKAEHPDLIILDLMLPELDGLEVCKRIRLNQETALLPILMLTAKAEESDTVIGLE
                     LGADDYVTKPFSPKALVARVKALLRRLERNANIPQTQYHYGPLTVDLSRHEVRLDGQE
                     VALTAKEFGLLEHLLRNIGRVLTRDVLLSAVWGYDYYGTTRTVDVHVRRLKQKLPLLD
                     DAIISIKSLGYKLRESDVPA"
     gene            1289..3148
                     /locus_tag="PK614_04640"
     CDS             1289..3148
                     /locus_tag="PK614_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81529.1"
                     /translation="MNLGIRWKVALGTLAAVFVGLAVAGWLVIRSVEQTELSRMAETL
                     ETSSGLAAMTLRPLFDQSGSTVPGPLLHATVRDLSQQAHLRITVIRQDGTVVADSAVP
                     TEGLAHVENHLSRPEVAQALASGRGMDIRASQTTGERTYYVARLLSAATPAQPGISVI
                     RLGLPLTSIDERVRHIQQDLATAFGAAFLLAMTLSLWVSRNLTKPLSEMAAAARQLAG
                     GTPGVRLVVSSTDEVGLLAQTLNQMTDQLETKIKEVSDDRAQLLAMLIAMVEGVMVLD
                     YRGTVVQVNPALERMFSLELTESRGRHYAELIRHEGLTALVSSVLQTRSGQGGEITLS
                     PSGRCLRVEASIAGGSREQEACAVFVFHDITELRRLEKIRKDFVANVSHELRTPLTSI
                     KGYVEALLDGGKDEPATATAFLEIIMRQSNRLNLILDDLLQLSQIESGQVLFRREPVD
                     LRALLERTVAVIKPLADKKHHTIELSLPDEYWVVEGDEERLVQVFINLLENAVKYTPD
                     QGRISMAIRNATHLRTTPPRAMIEIVVADSGIGIPEADRPRVFERFYRVDKARSRELG
                     GTGLGLAIVKHIVEAHSGQVWVEGNTPRGSRFVVHLPVAQESSTAVSTETGSR"
     gene            complement(3114..4106)
                     /locus_tag="PK614_04645"
     CDS             complement(3114..4106)
                     /locus_tag="PK614_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRB81530.1"
                     /translation="MAELSGLLLVVVILALLFDFSNGWHDSANAIATVVSTRVVSPST
                     AVLVAGVLNVAGAFMSTAVAKMVGTGIVDPQSVTQAVVASALAGAIVWNFVTLLLGLP
                     TSSSHALIGGLVGAALTHGGTAAVKFSGLRSVLEAMVLSPFFGFAIGLVLMVILSWVF
                     FRVPRALALRLFSRMQLVSASFMAFSHGANDAQKAMGIITLALVSAGVLPTAEVPTWV
                     IGSCALAMGLGTAVGGWRIVRTLGMRIVKLEPVHGFAAETGAAIVLLATAHIGLPVST
                     THTITSTVMGVGAVKRLSAVRWGVTRRILSAWLFTLPGAALLSTICYLFLSKLQ"
     gene            complement(4170..4787)
                     /locus_tag="PK614_04650"
     CDS             complement(4170..4787)
                     /locus_tag="PK614_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRB81531.1"
                     /translation="MFGLIPREEAFFDLFKNAAHNMIEGSRLLKDMTEDFRNPVEKAK
                     RIKDVEHVGDGITHEIARKLNQTFITPIDREDIHDLASALDDILDVVEAIADRFVLYK
                     VTAPTESAIRLANVLYQAAVEVGATVDLLGKSHQAVTECSVRVNSLENEADRISRDAI
                     SLLFEKETDPIAVIKWKEIYENFEAGTDRCEDVANILERIALKHT"
     gene            complement(4812..5804)
                     /locus_tag="PK614_04655"
     CDS             complement(4812..5804)
                     /locus_tag="PK614_04655"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRB81532.1"
                     /translation="MKRSRIVGTGSYVPSRVVDNQEVAAPLGLDPDQIVALTGIRTRY
                     WVEQGQASSDLAVVAGQRACEAAGITPQSVDAILVSTTSPDSAFPSTACHVQRALGAN
                     PVMAFDLSASCSGFLYGVSMADVMIRSGQVRSCLVIAAEVKSRSLDARDRETAMLFGD
                     GAGAVLVVQEDSAKPDAPGILGVRLYADGSGHGLITIPAGGSRKPVGVDTVRASEHML
                     RMQGRAVFRSAVRRLEQAMIDVLKEFGLAAGDVTRVIAHQANARILEQLRRRLGLSQE
                     AVYSVIEQYGNTSSASLPIALDQAVRTQRLAAGDLVLLGAFGGGLTWATGLVRW"
     gene            6010..6927
                     /gene="bamD"
                     /locus_tag="PK614_04660"
     CDS             6010..6927
                     /gene="bamD"
                     /locus_tag="PK614_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRB81533.1"
                     /translation="MHRHSRGLCVGLTIVVGMLWLAAGCSSKPKTTADAKPVSGTDEQ
                     IFLGDTIEKNYDPNVIMKRGEAFFDKEEFAEAIVEYQHFLELHRAHQLAVYAQLRLAE
                     SHLRMAKSIDRDPEPIQKAIAAFEKLRKDFPGSKYEAQALQRIADCHDWLAQTHLFVG
                     QFYYRRASYLAAAHRFDQIMKDYPDKKVAPEALYYLALTYQELGADDWAMEKLQLLAE
                     KYPNSENAGDSRRLLAKLEKKSPPAPTVVAKTDTPAPQNQPSTTLLAASVPSSSFTPS
                     ATPSIPNLKNSPALSLGQPFVSCRLGAWC"
     gene            complement(7106..8314)
                     /locus_tag="PK614_04665"
     CDS             complement(7106..8314)
                     /locus_tag="PK614_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRB81534.1"
                     /translation="MADRQLATTYDVIIVGMGPAGAVAAAALCREGLTVLGFDKDVHP
                     RYKVCGGGLSARIDLLLEPGFKSVVEQTVNGVQFVYRGQDPLLIESSQPIAYMVMRDR
                     FDHYLVQQAIQAGTELRTGEGVVEVTQDSDGVNVVTQEGRYRAKMVIGADGANSLVAR
                     QLFPNRSLYRAPALESEVRLGNSRHYPSATTILVDVGAARQGYGWIFPKRNGVSVGVG
                     EFRRKSSGLRTTFDRFVSEARGLSGWTVPRPVGHPIPAYSDAEGGHGDGRGSRFVNGR
                     AALVGDAGHLVDPLFGEGIYYAVRSGQLAARSILGNVHDPRESLAAYEAALERDILPD
                     FRVTARIARVIYAFPRLGFRLLRRYQDVVQSYFEVLQGRTTAAQFLPDAKKRLKASVN
                     DLLLEALHLR"
     gene            complement(8434..9180)
                     /locus_tag="PK614_04670"
     CDS             complement(8434..9180)
                     /locus_tag="PK614_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRB81535.1"
                     /translation="MTDSVQSISLVAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDVSERNRFRKAIILNSLLFIAGFSTVFVAFGASASLLGQALITYQEHLRRFGGIVV
                     IIFGLYLLGILNLNFLKMEHRYQFRNRPAGFLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTESMLSGVVLLTFYSLGLALPLFLTALGVDRFLSYFKEVRAYLWGVSTVSGVLL
                     IVVGVMIYANSLTMITSFLERYGIGWYLGQ"
     gene            complement(9183..9704)
                     /locus_tag="PK614_04675"
     CDS             complement(9183..9704)
                     /locus_tag="PK614_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB81536.1"
                     /translation="MMLCGGFSADARGALTSSPAVPMLVVERIPQVDEEAPNFLLRDS
                     EGFNVTLDQFRGKVVLLNFWATWCGPCKIEMPAMERLYRSFSRKDFEILAVSTDAQGA
                     AVTRPFQREVGFTFPVLHDADFRIGLQYGARQLPMTFLIDRKGIIRNRIPGARDWSAP
                     AGKRLVESLLQGS"
BASE COUNT         2200 a         3187 c         2780 g         1750 t
ORIGIN      
        1 gatctggccc agcgtcccga tcacctccgg gtgtcccgca tggccgataa ggatcaattc
       61 atacccttgc gtatagtcgc ggttcacttc gttatgcacc ttgatcacca gcggacaggt
      121 ggcatcgatc acgtgcaggc ggcggctctg cgcctcctcc cacaccgatt tcgccacacc
      181 gtgggcactg aagatcacga ccgatccttc cggcacttca ttcagctctt ccacaaatac
      241 ggcgccctta tgccgcagcg agttcacgac gtgccggctg tggacgatct catggcgcac
      301 ataaatcggc gcgccgtatt ttttgagcga caaatcgacg atatctatgg cgcggtcgac
      361 acccgcgcag aatccacgag gattggctaa ataaatcttc atctgctggt tccctctcac
      421 gctcatgaag ggccacccgg cctgccggga aaagcgcgta gtctcactga acgatcacct
      481 tacgtcagac ccgcccttgt cgtcaaccgg atcatctgcc ccgttccacc gcaaaagcag
      541 ttgaggccgc ggaaccattg tgggagaatc ccgccatgcc tccccccaca acgacacata
      601 aaaaaattct gatcgtcgaa gacgagaagg acattctcca actcgtcaag ctctatctgg
      661 agaaggaagg gttccgcacc gtctccgcga tgaccggcac agaggggctc cgccaggtca
      721 aagccgagca tcccgacctg atcattctcg acctcatgtt gcctgaactc gacgggctgg
      781 aagtctgcaa acgaatccgg cttaatcaag agacggccct gcttcccatt ctcatgctga
      841 ccgccaaggc ggaagaatcc gacacggtca tcggcctcga actgggcgcc gacgactacg
      901 tgaccaaacc cttcagcccc aaggcgctgg tggcgcgcgt caaggcgctc ctccgacggc
      961 tggaacggaa cgcaaacatc ccccagacgc aatatcacta cggccccttg accgtggatc
     1021 tctcccggca cgaggtccgg ctggatggac aggaagtcgc gctcacggcc aaggagttcg
     1081 gcctgctgga acacctgctg cgcaacatcg ggcgggtgct gacgcgcgac gtgctgctca
     1141 gcgccgtgtg gggctacgac tactacggca ccacgcgcac ggtcgatgtc cacgtgcgcc
     1201 ggctcaaaca aaaacttccg ctgctggacg acgccatcat ttcgatcaaa tcgctcggat
     1261 acaaactacg cgaatccgac gtacctgcat gaacctcggc atccggtgga aagtcgccct
     1321 gggcaccctc gccgccgtct tcgtcggctt ggccgtcgcg gggtggctgg tcatccgctc
     1381 ggtggagcaa actgaactca gccgcatggc ggagacgctg gagaccagca gcggcttagc
     1441 cgccatgact ctccggccgt tattcgatca gtccggatcg accgtaccag gccccctgct
     1501 ccacgccacc gttcgcgacc tgagccagca ggcccacctc cgcatcaccg tgatccggca
     1561 ggacggcacc gtcgtcgccg acagcgccgt ccctactgaa gggctggctc acgttgagaa
     1621 ccatctgtcc cgacctgaag tggcccaagc gctggcgtcg ggacgcggga tggatatccg
     1681 ggccagccag accaccggag aacgcacgta ttatgtcgcc cgcctgctgt cggccgcgac
     1741 gccggcgcag cctggcattt ccgtcatcag actcgggctt ccgctgacct cgatcgatga
     1801 gcgcgtgcga cacattcagc aagacttagc gacggcgttc ggcgcggcct tcctgctggc
     1861 catgaccctg agcctctggg tgtcgcgcaa tctcacgaaa ccgctctcag agatggccgc
     1921 agccgcccgc caacttgccg gcgggacgcc cggcgtccgg ctggtggtct cctccacaga
     1981 cgaagtcggc ctgctcgcgc agaccctcaa ccagatgacc gaccagctgg aaaccaaaat
     2041 caaggaggtt tcggacgacc gcgcgcaact gttagccatg ctgatcgcca tggtcgaggg
     2101 cgtcatggtg ctggattatc gcggcaccgt cgtccaagtg aatccggcgc tggaacggat
     2161 gttctctctg gaactgacgg aatcgcgggg ccgacactat gccgaactca tccggcacga
     2221 agggctgacc gcccttgtct cctccgtgct acagacccga tccgggcaag gcggcgaaat
     2281 taccctctcg cccagcgggc gatgtctgcg ggtcgaagcc tcgatcgccg gaggcagccg
     2341 cgagcaggaa gcctgcgccg tatttgtgtt tcacgacatc acggaactgc gccggctgga
     2401 aaaaatccgg aaagatttcg tcgccaacgt gtcgcacgaa ctgcgcacgc cgctgacgtc
     2461 catcaagggc tatgtcgagg cgctgctgga tggaggcaaa gacgagccgg cgacggcgac
     2521 ggctttcctg gaaatcatca tgcgccagag caaccggctg aatctgattc tggatgacct
     2581 gctgcagttg tcgcaaatcg agtccgggca ggtcctgttc cgtcgtgagc cggtggactt
     2641 acgggcgctg ctggaacgaa ccgtggcggt gatcaaaccc ctggcggaca agaaacatca
     2701 cacgatcgag ctgtcactcc cggacgaata ttgggtggtc gagggtgatg aggaacggct
     2761 ggtgcaggtc ttcatcaacc tgctcgaaaa cgcggtgaaa tacaccccgg atcagggacg
     2821 gatctccatg gcgatccgca acgccacaca cctccggacg accccgcccc gcgcgatgat
     2881 cgagatcgtc gtagccgact caggaatcgg catccctgaa gcggaccggc cgcgtgtctt
     2941 cgagcgattc taccgggtcg ataaagcccg ctcgcgcgaa ctcggcggga ccgggctggg
     3001 cctcgccatc gtcaagcata tcgtggaagc ccatagcggg caggtttggg tggaaggcaa
     3061 tacgcccaga gggagtcgat tcgtggtcca cctccccgtc gcgcaggaat cgtctactgc
     3121 agtttcgaca gaaacaggta gcagatagtc gacagtagcg cagcccccgg caaggtgaag
     3181 agccacgccg acaggattcg tctggtcacg ccccaacgca ccgccgagag ccgcttgacc
     3241 gccccgacac ccatgacggt ggaggtgatg gtatgggtcg tgctgaccgg cagaccgata
     3301 tgagccgtcg ccagcaacac gatagcggct ccggtttcag ccgcgaagcc atggaccggt
     3361 tccagcttca cgattcgcat gccgagggtt cgcacgatcc gccacccgcc gaccgccgtg
     3421 ccgagcccca tcgccagggc gcaggaaccg atcacccagg tgggcacttc cgcagttgga
     3481 agcactccgg cggacaccaa cgccaacgtg atgataccca tggccttctg cgcatcgttc
     3541 gccccgtggc tgaatgccat gaaactggcc gagaccagct gcatgcgtga gaacagccgc
     3601 aaggccaggg cgcgcggcac ccggaaaaat acccaactca ggatcaccat cagcaccagc
     3661 ccgatggcaa acccgaaaaa tggagacaac accatcgcct caagcaccga gcgcaggcct
     3721 gagaacttga cggccgccgt gccgccgtgg gtcagcgcag caccgaccag ccccccgatc
     3781 agcgcatgag aagagctggt cggaagtccc agtaacaggg tcacaaaatt ccacacaatc
     3841 gccccggcca aggccgaggc caccaccgcc tgcgtcaccg actgcggatc cacgatcccg
     3901 gttccgacca tcttggcaac ggcagtcgac atgaacgccc cggccacatt gagcacaccc
     3961 gccaccagca ccgccgtcga cggactcacc acacgcgtcg acaccacggt cgcaatggca
     4021 ttggcgctgt cgtgccagcc gttggaaaaa tcaaagagca atgccagaat cacgacgacc
     4081 aacaacaacc cgctcagttc agccatgaat gccgattcct acccttgaat agtgatcagg
     4141 agtggtgtcg agggagacgc ggaggacgct cacgtgtgct ttaacgcgat gcgctcaaga
     4201 atattggcga catcttcgca ccgatccgtc cccgcttcga agttttcgta gatctctttc
     4261 catttgatca cggcgatcgg gtccgtctcc ttctcgaaca acagagagat ggcatcgcgg
     4321 gaaatgcggt cggcttcatt ctccaagcta ttgacccgca cgctgcactc tgtgacggcc
     4381 tggtgtgact tgcccagcaa gtccacggtc gccccgacct ccaccgccgc ctgatacaac
     4441 acgttcgcca gccggatcgc cgactccgtg ggagccgtca ccttgtacag gacaaatcgg
     4501 tcggcgatgg cctcaaccac atccaggata tcgtcgaggg cactcgcgag atcatgaata
     4561 tcctctcggt cgatgggcgt gatgaatgtc tggttcagct tcctggcaat ctcatgggta
     4621 ataccgtccc cgacatgctc aacgtccttg atgcgtttgg ccttttcgac aggattccgg
     4681 aaatcctccg tcatgtcttt caggagacga ctcccctcga tcatgttgtg cgccgcattc
     4741 ttgaacaaat cgaaaaacgc ttcttcccga ggaatgagac cgaacataac gtcctttcta
     4801 gccgcgaggg gttaccagcg caccagtcct gtcgcccagg tcaagccccc gccgaatgcg
     4861 cccaacaaca ccagatcccc tgccgcaagt cgctgtgtcc gaaccgcctg atccagcgcg
     4921 atcggcaggg aggccgacga tgtattccca tactgttcaa tcaccgagta gaccgcctcc
     4981 tgcgagaggc cgagacgacg acgcagctgc tccaaaatcc gcgcattggc ctgatgggca
     5041 atgacccttg tcacgtcgcc ggccgcaagt ccgaactcct ttaagacatc gatcattgcc
     5101 tgctccaggc gacgtaccgc cgatcgaaac accgcgcggc cttgcatacg cagcatgtgc
     5161 tcactcgcac ggacggtgtc caccccgaca ggcttccgcg accccccggc gggaatcgta
     5221 atcaggccgt ggcccgagcc gtcggcatag agccgcacac caagaatgcc cggcgcgtcc
     5281 ggtttggccg agtcctcctg cacaaccagc accgctccgg ctccatcgcc gaagagcatg
     5341 gcggtctctc tgtccctcgc atcgagagac cgtgatttca cctcggcggc aatgaccagg
     5401 caggagcgaa cctgcccgct ccggatcatg acatccgcca tcgatacccc atagagaaac
     5461 cccgagcagg aagccgacag gtcgaatgcc atgaccggat tcgcacccag cgcacgctgg
     5521 acatggcagg ccgtcgaggg aaaggcgctg tcgggagagg tcgttgaaac aagaatcgca
     5581 tcgacagact gtggagttat cccggcggcc tcgcacgcgc gctgcccggc gactaccgcc
     5641 aagtccgacg acgcctggcc ctgctctacc caatacctgg tccgaatccc ggtcaaagcg
     5701 acaatctggt ccgggtccag gccgagcgga gcggctacct cctggttgtc tacaacccgg
     5761 gagggcacgt acgaaccggt tccgacaatg cgactgcgct tcacggggtt cctattcact
     5821 cgcctttgga gaccgtctgg gatgacatgt caggcgacct cacgaggtgc ggcgagatta
     5881 taggacccag gcccaggagg gtcaacctcc acaagccgaa ttcgttgctt ttcgcttcct
     5941 gatctgctag aaaaattgtg tagttacgat cagtccacgt tctcgactat ttaccgaagg
     6001 agtcgctgga tgcacaggca ttcaaggggg ttgtgtgtcg gtctcaccat tgtggtggga
     6061 atgttatggc ttgcagctgg atgctccagc aaaccgaaga cgaccgccga cgccaaaccc
     6121 gtaagcggca cggatgagca gatcttcctc ggcgacacga tcgagaagaa ctacgatcct
     6181 aacgtgatca tgaagcgcgg cgaggcgttc ttcgacaagg aggagtttgc cgaggccatc
     6241 gtcgaatacc agcatttcct ggaattgcat cgagcccatc aactggccgt ctatgcccag
     6301 ctccgtttgg cggaaagcca tctccggatg gccaagtcga tcgaccgaga cccggaaccg
     6361 atccagaagg ccatcgccgc gtttgaaaaa ctccggaagg atttccctgg cagcaagtat
     6421 gaagcgcagg cgctccagcg tattgccgat tgccacgact ggctcgctca gacgcacctg
     6481 ttcgtcggtc agttttacta tcgtcgagcc tcctatctgg ccgccgcgca tcgattcgat
     6541 cagattatga aagactatcc ggacaagaag gtggcgccgg aggccttgta ctatttggcc
     6601 ctcacctatc aggaactcgg tgccgatgat tgggccatgg aaaaacttca acttctagcc
     6661 gagaagtatc cgaacagcga gaacgccggc gatagccgcc gtctactggc caagctggag
     6721 aagaaatcgc cacctgcgcc gaccgtcgtg gccaagaccg acacaccggc tccgcagaat
     6781 caaccttcga cgacactgct tgcggcctcg gtgccatcgt cgagctttac gccttctgct
     6841 acacccagca tccccaacct gaagaattcg ccggccctct cccttggcca accgttcgtt
     6901 tcctgccggc tcggcgcctg gtgctgatag gcgtcgaacc tcccgttcat ccacccatgt
     6961 acaggccgtt caaaacacct cccgcaagac tgcagcgaag tcgacgtcgc gacgaataag
     7021 gcgggccacg tctgccgact ccgtcgggac ggtgaggcgc cggtgtcata cgagaacgcc
     7081 attggcggac gtgtccacca gcctgttacc gtagatgtaa tgcttcgagc agcaggtcgt
     7141 tgaccgacgc cttgaggcgt ttcttcgcat cgggcaagaa ttgtgcggct gtggtacgtc
     7201 cctgcagaac ttcgaaatag gactgcacca catcctggta gcgtcggaga agcctgaagc
     7261 cgagtcgggg gaaggcatag atgaccctgg cgattctggc cgtgactcga aaatccggga
     7321 gaatgtcacg ctccaacgct gcctcgtagg cggcaagcga ctcacggggg tcatgaacgt
     7381 tccccaaaat cgaccgggct gcgagctggc ctgaacgaac cgcgtaataa atgccctcgc
     7441 caaacagcgg gtcgaccaag tgaccggcat cacccaccaa tgccgctcga ccattgacga
     7501 atcgagagcc tcttccgtct ccatgcccgc cttcggcatc tgaataggcg ggaatcggat
     7561 ggccaaccgg acgaggcact gtccagcctg acagcccccg cgcttcgctg acaaaccgat
     7621 caaaggtcgt gcgcaaaccg ctcgatttgc gacgaaactc tcctaccccc acagacacgc
     7681 cattcctctt ggggaaaatc catccgtacc cctgacgggc agcccccaca tccacaagga
     7741 tggtggtcgc gctcggatag tgtcggctat tcccgagccg cacctcactt tcaagtgccg
     7801 gcgcccgata cagggagcga ttgggaaaca gctgccgcgc aaccagacta ttggctccat
     7861 ccgcaccgat caccatcttt gcgcgatacc gtccctcctg cgtcaccacg ttcacaccat
     7921 cggaatcctg agtgacctcg accaccccct caccggtccg gagctcggtt ccggcttgaa
     7981 tcgcttgttg cacaaggtag tgatcgaaac gatcgcgcat gaccatgtag gcgatcggtt
     8041 gagaggattc aatgagcagc ggatcctgcc cacgatagac aaactggacg ccattgaccg
     8101 tctgttcgac aaccgatttg aagccgggct ctaagagcag gtcgatgcgc gccgagagcc
     8161 caccgccgca taccttgtac cggggatgga cgtccttgtc gaaaccgagc accgtcaagc
     8221 cctcacgaca gagagccgca gcagcaaccg ctcctgccgg ccccattccg acaataatca
     8281 catcgtaggt tgtggccaat tgcctatcag ccatgtctca gctcccagtt gaaggacaca
     8341 gagctccgac ctggccttgg ccaagggtag aacacgcact gtaacgcagg cgtcaagggg
     8401 gagcccgata ggaaagggaa agacaggccg gagttactga ccgaggtacc agccgatccc
     8461 atagcgttcg agaaagctcg tgatcatggt cagcgaattg gcatagatca tcacacccac
     8521 cacgatgagc aacaccccgc tcaccgtcga cacaccccag agatatgcgc gcacttcttt
     8581 aaagtaactg agaaatcgat cgacgccgag cgcggtgaga aacagcggta gggctagccc
     8641 cagcgaataa aacgtgagca ataccacacc actgagcatc gattctgtcg tactggcata
     8701 gagcaggatc gtccccagga caggtcccac gcagggagtc cagcctgcag caaacgcgat
     8761 gccgatcagg aacgaaccca gaaacccggc cgggcggttt cggaattgat accggtgctc
     8821 cattttcaga aagttcaaat tcaagatgcc gaggagatag aggccgaaga taatcacgac
     8881 gatgcctccg aaccggcgca gatgctcctg gtaggtaatc aatgcctggc ccaacaggct
     8941 ggccgatgcc ccgaacgcga caaacaccgt cgaaaaccca gcgatgaaca gcagggaatt
     9001 caaaatgatc gctttccgga agcgattccg ttcagagacg tccgtgagct gttcgatcga
     9061 gagtccggta atgtaggaaa tgtacgaggg gacgagcggc agcacacagg gcgacacgaa
     9121 cgacagcagc ccggcggaga atgcggcaac caatgaaatc gattgcaccg agtcggtcat
     9181 ggttaggacc cctgcaacag cgattcaacc aggcgcttcc ccgcaggggc gctccagtct
     9241 ctggcccctg gaatccgatt gcgaatgatg cccttgcgat cgatcagaaa cgtcatgggg
     9301 agctggcgtg cgccatactg aagcccgatg cgaaaatcgg cgtcgtggag cacggggaaa
     9361 gtaaagccga cttcccgctg aaagggacgg gtcaccgctg cgccctgggc gtcggtcgag
     9421 accgcgagaa tctcaaagtc cttgcggctg aagcttcgat acagtcgctc catcgccggc
     9481 atctcgattt tgcagggccc gcaccaggtc gcccagaaat tcaagagcac gactttccct
     9541 cggaactgat cgagcgtgac gttgaacccc tcggagtccc gaaggaggaa gttcggagcc
     9601 tcctcatcga cctgaggaat gcgctcgacg acaagcatcg gcacagccgg cgaagaagtc
     9661 aatgccccgc gagcgtcagc agaaaaccct ccacagagca tcatgaccgc accggccata
     9721 gataatattg agcagcgcgt catcatcacc gttacgacgt tggcagcgcc tgagcagggg
     9781 tcgcatgggc ggccttctcc gcaggcttga ggagttgggt caacacctta aggttatcca
     9841 gtcgtgtcca atcccgaggc ccgatcactt tttcacgcag gacaccctgc tgatcaatga
     9901 tgtaggtttc ggggaca
//
LOCUS       DAORQN010000048         9807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_374_length_9807_cov_33.682527, whole genome shotgun sequence.
ACCESSION   DAORQN010000048 DAORQN010000000
VERSION     DAORQN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_9807_cov_33.682527"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..603)
                     /locus_tag="PK614_04680"
     CDS             complement(112..603)
                     /locus_tag="PK614_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81537.1"
                     /translation="MSHRTVLSFALLAVLVGGIHGCTTMQAGSTTTLPSSAFFPVDAN
                     ELKSLQTIAHAQDSRMKNCHKGPACEDAYYTRGLVALFENRADAITVFQELHTAMPNS
                     RYEAATTGWLNLLQDTAPSSKHHQALMVQLKQEVLHNLLERPDLTALRTAKDHDRRVA
                     ELK"
     gene            complement(911..7999)
                     /locus_tag="PK614_04685"
     CDS             complement(911..7999)
                     /locus_tag="PK614_04685"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRB81538.1"
                     /translation="MATEVEYALMAGRVYQSTRGKINWLPDLQSWGWTEQQLQQQPSG
                     FEAVSFQKGNEIVVSFAGTGSNIDWWANAGGFFGVTTEQLRQAADYYLQVKATHPGAI
                     IHLTGHSLGGGLASLMAVFFDETAVTFDQAPFRNSASGSVATSLKDYLLNERGYSQAA
                     LQGLTNFISAAASGGVPHNNNVIDFSVQGEILSAASGLRIGTQTSWTHGVPDLIPIDL
                     HSHALLTAFLQSDRTAPAQQSLSDVTFKLPDVVRMIFDSNLYKFSTGTQNTQNKNFIE
                     HLVHHQNGIAGLTVGETVIPADAMLTRFTSDLWKLAQDGGLTMADGPTPATNLVSKTL
                     IAFAMQKYYDETQASAGYNQELFTDVTGGVHFDRADVAATLGDAKGYNDFHFYLVNNF
                     SAADRQRIEDLLPVLRDWYVQAGAGGMNATDIQNRGAFMLGGYRTDTLIGGSQTDLLV
                     GNVGGDKLTGGGGDDTLLGGTGFDTYYYTTGDGHDRIEDSDAEGVIFVNGQMLVGGVK
                     KVGQTYWESADGTIKYEMSGTDLVVKLNGTQILTVNENFESGQFGINLIDRSSAPRNT
                     GVPTGPFRMVWTGTAQPESLGDPTLSPHAGDAVFGFEGGDFLYGDGFGNDLLDGGEGD
                     DFLYGYNGDDYLDGGMGNDYLDASDSVNIATIHDGYDILIGGAGDDVLYAGGVIEDIG
                     ELDYLDGGVGADYLWAGRGMDVLLGGVGNDILLGDNRPSGGPIPFNNSGSMILVPGPA
                     IFRVTGGDDSLEGGDGNDILVGDGGDDVLLGGDDNDELWGDDQQVAVVQEGNDWLDGG
                     AGNDQLVGGGGQDTLAGGDGDDLLAGDYANNSTDGFDDILDGGAGIDELQGGGGHDLL
                     TGGTENDRLFGQDGNDSLFGDTGDDLLTGGLGNDELDGGEGIDDMQGREGDDLLLGGG
                     GSDFLYGDGPDPTVLNLVGGNDSLDGEEGDDLLWGGGGQDQLFGGDGADQLVGDNGND
                     HLYGEAGNDLLVGDSTLFPAQAGTDLLDGGDGNDVLQGGGGDDELEGGDGADVIFGEF
                     DNVLGAPAGNDDLHGGLGNDRLVGGGGLDTYRFNLGDGRDSIEDNAVQSNRVVFGAGI
                     TAEDLSLDVATGDSLLVRVGNNGDALEIVGFGISLPTDFHSIRQFEFADGTILSDSQL
                     LARGFHLSAPVTGGTVLGTAYADHIEGSQVDDWIHGRDGNDILNGGQGNDVLEGEEGD
                     DELNGGSGNDRLYGSAGSNVLRGGEGDDLLDSAGVGDQLFGGIGDDGYHLRSGSQTLN
                     EELNAGTDTIYLTPAGSLTFYTPDNVENVRIEDDVFLDPVLQVNMVGNALDNVLSGSH
                     RLDGQAGNDTLIGLGDNTFVFGRGYGHDIVQAGTQWYAHTGLDQVEFVADIAPADLVL
                     ENHANDLVVKVNGTTDQLTVQSYFVSPSATVDQFVFAGGTVWGLTEIESRVQTFVGSE
                     ADETFYGTVNDDTISGLGGNDQIRASSGNDTLDGGTGNDFLEGWSGNDTYVFGLGYGQ
                     DSIDEQGDSADVDTLRLEGINPGDITLRATPDFMTDAILTINNTADQLTLAGFFAFDS
                     LRVDRFQFADGTVWDYNAMLAHTEGVTLDGTEDADFLYGNVTNDMLAGFGGDDSLNGG
                     AGNDTLDGGSGVDTMAGGTGNDVYVVDDPGDVVTEQASQGTDTVQAAITYTLGANLEH
                     LTLIGTAAINGTGNARNNTLTGNSAANVLTGGTGNDTYVVGIGDTVVEQASSGTDTIQ
                     SSVSWTLGTNVENLTLTGTSAINGTGNTLNNSLIGNTGSNILDGSSGTDAMSGGAGDD
                     IYVVDNTADTVTENASAGIDTVQSSVTYTLGANVDNLTLTGTAAINGTGNGLNNVLTG
                     NSVANVLNGGTGADTMNGGAGNDTYVVDNPGDVVTEAPGGGTDLVQSSITYALAAEVE
                     NLTLTGILAINGTGNTLNNSLTGNTGNNILDGGAGADAMSGGTGNDTYVVDNTGDTVT
                     EAASAGTDTVQSSVTFTLGSNIEKLTLTGTAAINGTGNTLNNTLIGNSGANTLNGGAG
                     TDAMSGGAGNDTYVVDNAGDTVTENTTEGIDTVQSSLTHTLAVNVENLTLTGTTAING
                     TGNALDNVFTGNSGANVLTGGAGNDTYVVGTGDSIVENAGAGIDTVQSSIAWTLGANL
                     ENLTLTGTGAINGTGNTLDNVLTGNSGANVLTGGAGNDTYVVGTGDSVVENTSAGTDT
                     VQSSITWTLGANLENLTLTGSAVINGTGNVSNNALGGNSANNTLSGLGGNDTYLYSRG
                     SGQDTVIDNAGTADSVLFGATINPLDLVLSRQANDLRLAIHGSTDQVTIQNWYGGATN
                     QTETIQAGNGQTLLNTEVDQLIQAMATFSQQTGLTWDQALDQRPQEVQTVLAANWH"
     gene            complement(8002..8778)
                     /locus_tag="PK614_04690"
     CDS             complement(8002..8778)
                     /locus_tag="PK614_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81539.1"
                     /translation="MSNQCTLVRLCKAICLVLTLGVSMNACATANAFTWKEEALLHDG
                     STIIVERSVERGGRHEIGQEPPIKEQSLTFTMHNTNEKITWEDGYTKDVGAANFLPML
                     LEAREDTAYLVVHPMGKLSHMKWGSPNPPYVIFKYQNNRWNRIALSELPSEFTRPNLI
                     FSSPDDEAKKLRHRIVPVEVIKRLYDGYTQPEYKSIVRTPLDHWKPRKSGRMVRTEDG
                     GWIGIDWFEIQPSLEACLAKCAREKVSVQDCPCHTLFEGK"
     gene            complement(8775..>9807)
                     /locus_tag="PK614_04695"
     CDS             complement(8775..>9807)
                     /locus_tag="PK614_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81540.1"
                     /translation="SLSDVTFKLPDVVRMIFDGNLYKFSTALNSNRENFLERLVRHQN
                     GITGNPEKGEVPVQADAMLTRFTSDLWKLAQDGGLTMTDGPTPATNLVSKALIAFAME
                     MYYQDTANATNPTKELFTNLATENTGSGGGIRFDRADVTDTLTTAKGYNDFHFYLVNN
                     FSPADRQRIEDLLRLIRDWYVQAGQSGMNATDTQDRGAFMLGGHGMDALTGGVGTDLL
                     VGNAGHDSLTGSAGTDTLLGGEGFDRYYWNTGDGHDRIEDSDARGMILVNGQMLVGGI
                     KKAGHTDWTSPDGTITYQMSGTDLIVELNGTTIMTVNENFQSGRFGIRLMDGSGHVDG
                     AWVMERSAA"
BASE COUNT         2087 a         3062 c         2372 g         2286 t
ORIGIN      
        1 ccccccctgt cggccccccc gcgggggcgg ccgctggaca accgggcggg ggcgccgcgc
       61 ccccgcatgc cggccgaacg ggacaccagg ctcatagcta taactggttc attacttgag
      121 ctccgcaacc cgccggtcgt ggtccttcgc agtccgcaac gccgtcaaat caggacgctc
      181 caggagatta tgcagcactt cctgcttcaa ctgaaccatt aacgcctgat ggtgcttgga
      241 ggatggcgcc gtatcctgca agagatttaa ccagcccgtc gttgccgcct catagcggct
      301 gttcggcatg gctgtgtgca gttcctgaaa caccgtaatc gcatcggctc gattctcaaa
      361 gagcgcgacc aagccacgcg tgtaataggc atcctcacag gcgggacctt tgtgacaatt
      421 cttcatgcgg gaatcctgcg catgggcgat ggtttgaagc gacttcagct cattcgcatc
      481 aacggggaag aacgcgctgc tcggtaacgt ggtagtagac ccagcctgca tcgtcgtaca
      541 cccgtggatg ccaccaacga gaactgccaa tagggcgaaa gagagaaccg ttcggtgcga
      601 catcgtagac ctcctatgca gcgcggacat ggtggcgtgc agtgattggg ctgatttctt
      661 gcgttgcatc gatgttctga ctgtaccatc gaaccccggt tcggccatta cacggaagct
      721 gtacctcttc ggtactggac aaaaacgtac tacaaaatct cactgattga gggcttttca
      781 tggacagaag atctggaaac gagggtggcg acagagctgt gtcgagctga tggtgaaatg
      841 agagagggaa cgttgccgtg gtcagaaagg aaatcgcccc gccgggttgg cggggcgacg
      901 gaatcgtatt ctagtgccag ttggcggcga ggaccgtctg tacttcctgc ggtcgttgat
      961 cgagtgcctg atcccaggtc aaacccgtct gttggctgaa tgtcgccatc gcttggatca
     1021 attgatccac ctccgtattc aacaacgttt gcccattgcc ggcctgaatc gtttctgttt
     1081 gattcgtcgc acctccgtac cagttctgaa tcgtcacctg gtccgtgctg ccgtggatcg
     1141 caagtcgcag gtcattggcc tgtcggctaa gcaccagatc cagtggattg atcgtagccc
     1201 cgaagagcac gctgtcggct gtgcctgcgt tgtcgatcac cgtgtcctgg ccgctacctc
     1261 gactatacaa gtacgtatca ttgcctccga gcccactgag ggtattattt gcactgttcc
     1321 ctcccagtgc attattggag acattgcccg tgccgttgat caccgcgctg cccgtcaacg
     1381 tcagattttc gagattggcg cccaacgtcc acgtgatcga actctggacg gtgtccgtcc
     1441 ccgcgctggt gttttcaacc accgaatcgc ccgtcccgac gacataggta tcgttacccg
     1501 ccccaccagt caagacgttc gcgccgctgt tgccggtcaa aacgttgtcc agtgtattgc
     1561 ccgtcccatt gatagcgcca gtgcccgtca gggtcaggtt ctcaagattg gcacccaggg
     1621 tccatgcaat tgaactctga accgtatcta tccccgcgcc ggcattctca acgatcgaat
     1681 cgccagtccc gaccacgtag gtatcgttgc cggcgccgcc cgtcagcacg ttcgcgccgc
     1741 tgttgcccgt gaagacgtta tccagtgcgt tgcccgtgcc attgatcgcg gtcgtacccg
     1801 tcagagtgag gttttctacg ttcactgcca acgtatgcgt gagcgaactt tgaaccgtat
     1861 caatgccttc agttgtgttc tctgtgaccg tatctccagc gttatcgaca acgtaggtgt
     1921 cattgcctgc gccaccagac atcgcatcgg tgcctgctcc accgttcaac gtgttggccc
     1981 cgctgttccc gattagcgta ttgttcaggg tattgccggt gccattgata gctgccgtgc
     2041 ctgtgagcgt cagcttttca atatttgagc ccagcgtaaa cgttaccgaa ctctgcacgg
     2101 tgtccgtgcc ggcgcttgcg gcctcggtca cggtatctcc ggtattatcg accacatagg
     2161 tgtcattccc agtcccaccg gacatagcat cggcaccggc ccctccatca agaatgttat
     2221 ttcccgtatt gccggtgagg ctgttgttca acgtgttgcc cgtgccgttg atcgccaaga
     2281 tgcccgttaa ggtgagattt tcgacttcag ccgccaaggc ataagtaatc gagctttgca
     2341 ccagatcggt tcctcctccg ggcgcctctg tcacaacgtc tccaggatta tccacaacat
     2401 aggtatcgtt ccccgcgccg ccgttcatcg tatccgcacc ggtcccacca tttaggacat
     2461 tcgcaacgct gtttcctgtc aagacgttat tgaggccatt gcccgtgcca ttgatggcgg
     2521 ctgtgccggt gagcgtgagg ttgtcaacat tcgcgccgag ggtgtaggtc accgagcttt
     2581 gcacggtatc aatgcccgca cttgcgttct cagtgaccgt atccgcggta ttatcgacaa
     2641 catagatgtc atcgccggcc ccgcccgaca tcgcatcggt gccggaactt ccatccagaa
     2701 tattgctacc ggtattgccg atgaggctat tgtttagcgt gttgccggtg ccattgatcg
     2761 ccgacgtgcc ggtcaacgtg aggttctcca cattggtccc aagggtccag gatacggaac
     2821 tctggatggt atctgtcccg ctgctggcct gctccacgac tgtatcacca atccccacga
     2881 catacgtatc gtttccggtt ccacccgtaa gcacgttagc cgcgctattc ccggtgagcg
     2941 tgttgttacg ggcgttgccg gtgccattaa tggcggcagt cccgatcaac gtaagatgct
     3001 cgagattcgc gcccagcgta tacgtaatgg cggcttgcac cgtatcagtc ccctggctag
     3061 cttgctccgt taccacatct cccgggtcgt ccacgacata tacatcgttt cccgtgccac
     3121 cagccatcgt gtcaacaccg cttcccccat cgagcgtatc gttgccagct ccgccattca
     3181 ggctatcatc gcctccgaat cctgctaaca tatcgttggt aacattgccg tacaagaaat
     3241 cggcgtcttc tgtcccatca agcgtcacgc cttccgtgtg ggcaagcatg gcgttgtaat
     3301 cccacacagt cccatcagcg aattggaacc gatctacgcg gagtgaatca aaggcaaaga
     3361 acccggcgag agttaattga tcagcggtat tattaatcgt caggatggca tcggtcataa
     3421 aatcaggcgt cgcacgcaac gtgatatcgc ccggattaat tccttctagt cgaagggtgt
     3481 ccacatctgc ggaatcgcct tgttcatcga tgctatcttg accgtatccc agtccaaaca
     3541 cataggtgtc atttccagac caaccctcca agaaatcatt ccccgtcccc ccgtcgagag
     3601 tatcattgcc tgaggacgcc cgaatttgat cgttcccgcc gaggccgctg atggtgtcgt
     3661 cattgacggt gccataaaaa gtctcgtcgg cttcgctacc gacaaaagtc tgcacgcgac
     3721 tctcgatctc ggtgagcccc cacaccgtgc cgccggcaaa tacaaactgg tcgacggtag
     3781 cgctcggcga gacgaagtag gactgcaccg tcaattgatc ggtggttcca ttgactttta
     3841 cgaccagatc attggcatgg ttctccagca caaggtcggc cggtgcaatg tcagcgacaa
     3901 actccacctg gtcgagacct gtatgcgcat accattgcgt acctgcttgc acgatatcgt
     3961 gaccataacc acggccaaag acaaatgtat tgtccccaag cccgatgaga gtgtcgttcc
     4021 ccgcctgccc atcaagtcga tgggagccgg acagcacatt gtccaaggca ttcccaacca
     4081 tgttcacctg taagactgga tcgaggaaca catcgtcttc gattctgaca ttctcgacat
     4141 tatctggggt gtaaaaggtg agcgaccctg ccggcgtgag ataaatcgta tcggtcccgg
     4201 cattcagctc ctcattaagg gtctgcgatc ccgatcgaag atgataccca tcatcgccga
     4261 ttccgccgaa gagctgatcg cctacccctg ccgaatccaa gagatcatct ccttctccac
     4321 cgcgcaacac gttgctacca gcactcccat acaaccgatc gtttcccgat ccgccgttca
     4381 actcatcatc tccctcctcg ccctctaaca cgtcatttcc ctggccccca ttcaggatat
     4441 cgttcccatc gcgtccgtgg atccaatcat cgacttggct cccttcaatg tgatcggcgt
     4501 aggccgttcc aagcactgtt cctcctgtca ccggagcaga caaatgaaat cctcgggcga
     4561 gcagttgact gtctgacaat atggtgccat cggcaaactc aaactgacgt attgagtgaa
     4621 agtctgtggg caaacttatc ccaaacccaa ctatttcaag agcgtcacca ttgttgccta
     4681 cgcgcacaag gagtgagtca ccagtcgcca catccaagct taggtcttcg gctgtgatac
     4741 cggcgccaaa gaccacgcgg ttgctctgaa ccgcattgtc ctcaatcgag tcgcggccgt
     4801 ctcccagatt aaagcgatag gtatcgaggc caccgccgcc aaccagccgg tcatttccta
     4861 acccaccgtg aagatcatca ttgcctgctg gcgcccccag gacgttgtcg aattcaccaa
     4921 agataacatc ggcgccatcg ccgccctcca actcgtcgtc gccgcctcca ccttgaagga
     4981 catcattacc gtcgccaccg tcgagcagat cagtccccgc ttgagcggga aagagagtag
     5041 aatcgccgac caacaggtcg ttgccggctt caccataaag gtgatcgttt ccattgtcac
     5101 cgacaagctg atccgccccg tcaccgccga ataactgatc ttgcccccca cctccccata
     5161 acaggtcgtc gccctcttcg ccatccagcg aatcattgcc accgacaagg ttcagcacgg
     5221 tgggatcagg accgtcgcca tacagaaaat cactaccgcc cccgcccagg aggaggtcat
     5281 ccccttcgcg tccttgcatg tcgtcgattc cctctccgcc gtccagctca tcgttgccca
     5341 gtccgccggt tagcagatcg tctcccgtat cgccgaacag gctgtcgttt ccatcttggc
     5401 cgaacagccg gtcattctcg gttccaccgg tcaacagatc gtgtccgcct cccccttgca
     5461 actcatcaat cccggctccg ccgtcgagga tatcgtcgaa tccgtctgta gaattgttcg
     5521 cataatcgcc ggcgagcaga tcatcaccat cccctcccgc gagcgtatct tgtcccccac
     5581 cgcccaccag ctgatcgttc cccgccccgc cgtcgagcca gtcattgcct tcctgaacca
     5641 cggcaacttg ctgatcatct ccccaaagtt catcgttatc atcgccaccc agcaatacat
     5701 cgtcgccgcc atccccgacc agaatgtcgt ttccgtctcc cccctccagg gaatcatctc
     5761 ccccggtcac gcggaagata gccgggcctg gaactaggat catggagccg gaattgttga
     5821 acggaatcgg ccctcccgat ggacgattgt ctcctagtaa gatatcgttc cctactccgc
     5881 ccaacaacac atccattccc ctccctgccc acagatagtc tgccccaaca cccccatcca
     5941 gataatccaa ctcaccaatg tcttcgatta ctccgcccgc atataacaca tcatcgccgg
     6001 ctccgccgat gaggatgtca tatccatcat ggatcgtggc aatgtttaca ctatcgcttg
     6061 catccaaata gtcattcccc atccccccat cgagatagtc atccccattg tatccataca
     6121 agaagtcatc gccctctccc ccatcaagga gatcatttcc gaaaccgtca ccatagagaa
     6181 agtcaccgcc ctcaaaccca aagactgcgt caccagcatg tggagacaag gtaggatctc
     6241 caagggactc gggctgagca gtgcccgtcc acaccatccg aaaggggccg gtcggcaccc
     6301 cggtgttccg gggagcactc gaacgatcga ttaggttaat cccaaactgg ccgctttcga
     6361 aattctcatt caccgtcagg atctgcgtgc cgttcagctt caccacgaga tccgtccccg
     6421 acatctcgta cttgatcgtg ccatcagcgc tttcccaata ggtctgcccc accttcttca
     6481 ccccgccgac cagcatctgg ccgttgacga agatcacccc ctcggcatcc gaatcctcaa
     6541 tccggtcatg gccatcgcct gtggtgtagt agtaggtatc gaacccagtc ccgccgagca
     6601 aggtatcatc gcccccgccg ccagtcagct tgtcgcctcc gacattgccc acgagcaggt
     6661 cggtctggct gccaccgatc agagtatcag tgcggtagcc gcccagcatg aacgccccgc
     6721 ggttctggat gtcggtggca ttcatcccac ctgcccccgc ctgcacgtac cagtcccgca
     6781 gcacagggag gaggtcttca attctctgac gatcggcggc agaaaagttg ttcaccaggt
     6841 agaagtgaaa atcgttgtag cccttagcat cacccaatgt cgcggctacg tccgcccgat
     6901 cgaagtgcac gccacctgtc acgtcggtga acagttcctg gttgtagccc gcgctcgctt
     6961 gcgtttcgtc ataatacttc tgcatggcaa aggcgatcag ggtcttactc accaaattcg
     7021 tagcgggagt agggccatcc gccatcgtca ggccaccatc ctgcgccaac ttccagaggt
     7081 cactggtgaa gcgagtgagc atggcatcgg caggaatcac ggtttctccc actgtcaaac
     7141 cagcgatgcc attttgatgg tgaaccaggt gctcaatgaa gtttttgttt tgagtatttt
     7201 gtgtgccagt tgaaaactta tacaaattac tatcaaaaat catccgcacc acatccggga
     7261 gtttgaatgt cacatcgctc aatgattgct gggccggcgc cgttctgtcg ctttgcaaaa
     7321 atgcagtgag cagtgcgtgg gaatgcaggt caataggtat cagatccggc acaccatggg
     7381 tccaactggt ttgcgtgcct atcctgagcc cagaagccgc actcaggatt tcgccctgca
     7441 cactgaaatc aatcacgtta ttattatggg gaacgccccc gctggccgca gcgctgatga
     7501 aattagtcag cccttgtaac gcggcttgac tgtaacctcg ctcgttgagc aaatagtctt
     7561 tcagagaggt cgcgaccgaa ccactggcgg aattcctgaa aggcgcttgg tcaaaggtga
     7621 cggcggtttc atcaaaaaac accgccatca acgaagccaa tcctccacct aaggaatggc
     7681 cggtgagatg aatgattgct cctgggtggg tagctttgac ttggagataa taatctgccg
     7741 cttgccgaag ttgttcggtc gtcacaccga agaacccacc tgcattggcc caccagtcta
     7801 tattggaacc tgtgcctgca aacgagacaa cgatctcgtt gcccttctga aacgacacag
     7861 cctcaaagcc actgggttgt tgctgtaact gttgttccgt ccacccccat gactgaagat
     7921 cgggaagcca attaattttt ccacgagtgc tctgatacac ccgccccgcc atcagtgcgt
     7981 attcgacctc ggtcgccata attatttccc ctcaaataat gtgtgacatg gacagtcttg
     8041 cacgctaact ttctctcgtg cgcacttcgc caaacatgcc tccaaggaag gttgaatctc
     8101 gaaccaatct atcccgatcc aaccaccatc ttccgtgcga accatccttc ctgacttgcg
     8161 gggcttccaa tgatcgagcg gcgtgcggac aatgcttttg tactctggct gtgtgtaccc
     8221 gtcatacaac cgtttgatga cctccaccgg aacgatgcga tgtctcaatt tcttcgcttc
     8281 atcatcgggt gaagagaaaa ttaaattcgg ccttgtgaat tccgatggta gctcggagag
     8341 cgcgatacga ttccatctat tgttttggta tttgaagatc acataaggtg ggtttggact
     8401 tccccatttc atgtgtgaca actttcccat tggatgcacc accaaataag ccgtatcttc
     8461 acgcgcttct agcaacatcg gcaaaaagtt cgctgcaccg acatctttag tgtatccgtc
     8521 ttcccatgtt attttttcgt tggtattgtg catggtaaag gtcaagctct gctccttgat
     8581 cggtggctcc tgccctattt catgccgccc gcctcgctca accgaacgct cgacaatgat
     8641 tgtgcttcca tcgtgcaata gcgcctcttc cttccacgta aacgcgttgg ccgttgcaca
     8701 cgcgttcatg ctcaccccca aggtcagtac caagcagatt gccttacata accgcaccaa
     8761 cgtacactgg ttgctcatgc cgcgctcctt tccatcaccc atgccccatc gacatgccca
     8821 gagccatcca tcagccggat cccgaaccgc ccgctctgaa agttttcatt gacggtcatg
     8881 atcgtcgtac cgttcagctc cacgatcaaa tccgtccccg acatctgata tgtgatggtt
     8941 ccatcgggac tggtccaatc cgtatgcccg gccttcttga tcccgccgac gagcatctgg
     9001 ccattgacga ggatcatgcc ccgcgcgtct gaatcttcaa ttcggtcgtg gccgtcaccc
     9061 gtgttccagt agtagcggtc gaacccttcg ccgcccagca gcgtatctgt ccctgcactg
     9121 cccgtcaggc tatcatgacc tgcattaccg accagcagat cagtgccgac cccgccggtc
     9181 aaggcatcca taccatggcc gcccaacatg aacgccccac ggtcctgggt atcggtcgca
     9241 ttcatcccgc tttgtcccgc ctgcacgtac cagtcccgga tcagccggag gaggtcttca
     9301 atccgctggc gatcggcagg agaaaagttg ttcacgagat aaaaatgaaa atcgttgtaa
     9361 cctttggcag tagtcagcgt atctgtcacg tcagcccgat cgaatcgaat gcctcctccg
     9421 ctgcccgtgt tctccgtcgc caggttggta aagagttcct tcgtgggatt ggtcgcattg
     9481 gccgtgtctt ggtaatacat ctccatggca aaggcgatca gtgccttgct gaccaaattc
     9541 gtggcaggag tgggaccgtc cgtcatcgtg agtccaccat cctgcgccag tttccagaga
     9601 tcgctagtga agcgggtgag catcgcatca gcctggaccg gcacttcacc cttttccgga
     9661 tttcccgtga tgccattctg gtggcggaca agtcgttcga gaaaattttc acggttagaa
     9721 ttaagagctg ttgaaaactt atacaaatta ccatcaaaaa tcatccgcac cacatccggg
     9781 agtttgaatg tcacatcgct caatgat
//
LOCUS       DAORQN010000049         9758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_376_length_9758_cov_10.926208, whole genome shotgun sequence.
ACCESSION   DAORQN010000049 DAORQN010000000
VERSION     DAORQN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_9758_cov_10.926208"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK614_04700"
     CDS             complement(<1..469)
                     /locus_tag="PK614_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81541.1"
                     /translation="MPIENNFQHDELSRKNPGDRLTYADWTTPPDADSPAWQEAMSQL
                     GQKLSQAGARLIMFLHGAIPGTDVFGLGRLDEVGGLKRGYSRGISGLDSLLSFMREGT
                     NGITPLPGGMKPPLSNDEATKTLLDSQIGDAGNFTTTTVEQCRKALNQKLSTPI"
     gene            complement(542..1099)
                     /locus_tag="PK614_04705"
     CDS             complement(542..1099)
                     /locus_tag="PK614_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81542.1"
                     /translation="MSLTAGPIRTGWWPLCALLIASPATAADLTPAAVRDHADQTFQH
                     LHMQGRAESPPSAAQARPAQATSSAEELLTGQGQGDLSKGKLVCQRVSELAARTDIAK
                     QIRVLVKEHATDRLRERTGREAEQDIEVVREEIVQEYLQGVRIVERHTDEEAKTCSAT
                     AVMPKPRLSAPASTESNGRESAAHR"
     gene            complement(1099..2280)
                     /locus_tag="PK614_04710"
     CDS             complement(1099..2280)
                     /locus_tag="PK614_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81543.1"
                     /translation="MPQNRFDSGPRFIPHTSPRARSIHLFIGLLSIALSLLLTCPSPS
                     QAEVRVVTGTGEHRMNDHETKTDGIRLATEQAKKQALEQVASYLESVTVVRDLDVTLD
                     EIRSYTAGMVLVLDQHVTTALDGEFVVVRVELTAQADTDEVVQAIATLRQNEDAREQL
                     LALRRDVESLQQELEAANDALAGATSPEQAQGLSHERNELLNRAESDAMVAQAWTDWV
                     LLAPFAQPSPSAGLAQIHALLGLASRLNPDNPHLAIAQQTVGTKAPPAPPQPRRPPVP
                     HTVPFLPGYTVVPQQPAQPGAIEVPAQPGASLPQGSRGTMPTYRQVPPPTGRPAARTL
                     HTYRSGVDQAAPAAEQPSINAPRGTPPAIIYQVPKKPVPIPTTPSQQIPGNGTHQGGT
                     R"
     gene            2466..3515
                     /locus_tag="PK614_04715"
     CDS             2466..3515
                     /locus_tag="PK614_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRB81544.1"
                     /translation="MSRIRSILTVTGALCMVVGLGLALVAPSSQSVAFAARGGGEVAT
                     PVQAGITEHVILFVLEGLGQESLKSGAMPVLSSLVKDGAVTWSATAVAPARRLPTMAS
                     LVTGMPVAKHGITWNVFEFSRGYPRAPTVFDYLDLSGGRDSAIFYMDESLYQLAKPEP
                     YTDYQMCGPLRAECNPDRLVGYVRDYFKKATSGSGYGHAIPALPHLLVVHLPAPGRVG
                     ETQGWKSVAYKDALKAVDKAMGTVLDLYREHGLIKRTTVFATSLSAFGETQFSAGEVS
                     GEQAGNVPVVPWIASGVGIKAGHTIRQPVSIIDTGATVMRALGLTTYTEWESHPVEEI
                     FKTAFAAAPVSPLLQ"
     gene            3523..4014
                     /locus_tag="PK614_04720"
     CDS             3523..4014
                     /locus_tag="PK614_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81545.1"
                     /translation="MRSSSHKAFAGRQVFFWIVAVTCVLVLGPSERLLAAGPPPAYGK
                     EHTITIDPTPLVPQSWWQVPGVTPPLWVLDPETSDAYRTTETRELKLKPGQYKFISFT
                     FDFPFTVSLDGKLEYSKSLDQCVEGRGTQTLVVRCKRTYPHGGQRDAYYGNSEVGDGT
                     GTR"
     assembly_gap    4129..4138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4139..5370
                     /locus_tag="PK614_04725"
     CDS             <4139..5370
                     /locus_tag="PK614_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81546.1"
                     /translation="WSVRTRAGWGVAKIGGPKAIEALIVLFNDPIMEVRNEAVAAVVS
                     LGAGQLDRLLAAMKDERWRVREHAAKACGDLHDSRAVDALTFACRDRDGAVKSAAAEA
                     LGKIGDPKAIPALVKLFRDSSKIVRETAGIALVAIGQPSVDLLLETLKDKDFVVRCHA
                     ARALGGMTTDYQIGRSWVREPRVVDALIEALKDPDRAVREDATIALGMIGDPRAIDGL
                     LEAMKDGAVKRHAIASLGMIGDPRALPAVLAALKGKGVRQDGTPTPGCIVSEDAFIKE
                     AAATALGHFRDPRVIPDLIMLLKDGVLREKAAAALVLIGDSAIEPLISFLYDPKASEV
                     EAEGERVLSYASVRLTAKDSLRLLVVETLEHLGWTPPDEETSVDSSQADNLRVDRPLG
                     DIGRFGPSGDFAKGSVR"
     gene            5558..6640
                     /locus_tag="PK614_04730"
     CDS             5558..6640
                     /locus_tag="PK614_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81547.1"
                     /translation="MADTVSEQIAALSDEDWAIREEAATLLGSLNDARAVLPLTKLLR
                     DTDRAVREAAIGSLSALGSASVPALAGCLADPALQVQEAASAILASIADARVFRPLVE
                     ALGSRDWIVRMHAAKGLGRIGDPEAVSVLMPLLQDKVKAVREEASSALAAIGAAAVLG
                     LIQALEHDDWLVRLHAVEALGKLKSPDSVDPLLRALFNERDSAIREDVVRALGAIRDA
                     RAVDYLVTVMKEPGLRLLAVEALGHIGDPRAVPLLRRVVEGVPLGEPRDTATPCADGW
                     TDEMATMGMAARALGMIADGVAIPSLIVALRNTITRSEAAAALAKFGPAVIPSLLPML
                     AKEQDENIRYHVRETLTAVGWRPGRV"
     gene            6736..7836
                     /locus_tag="PK614_04735"
     CDS             6736..7836
                     /locus_tag="PK614_04735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81548.1"
                     /translation="MSKESIDTLVSELTHEEEWRRMRATAACLAGGPRAVQALTQALE
                     SGDVALRVEAAAMLSRIKDPAAGLSLVGLLRDSEETVRQAAFSALEQMAGNLDDETAA
                     GLVRNLHESPDEDVRNRVRQLLGVIPNAITPLCEMLTHPEVDAQTTAATILEHLLDPR
                     SADGLIDAMSSPAVRDTAVRTLKKLGAIRDRIDATFNALRDVEGASEREEARMSTVMD
                     LLGIGRPAVEILIEYLEDDDWVVREAAADLLGKIGDVRAVEPLMKRLQLDKDTGVKEY
                     AVKSLGLIGDPRPAQLYIEAIPIRPLRVMAIEALAKIKDVEVLRPYNELFNRLRTDRD
                     GIVSYSAGLIADKLAALEGEQPVGQEESDDHE"
     gene            7829..8590
                     /locus_tag="PK614_04740"
     CDS             7829..8590
                     /locus_tag="PK614_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81549.1"
                     /translation="MSDAERIAQLISALRDDNEALRDHAMASLGQMGIEAVPQLIGLM
                     ADEDVVIREAAATAVVRIGPVAFDQMLEALSDDEWAIREQAANVLGRFRDPRAVDPLM
                     VALKDKDGAVRTAAVWALERIGDSRATPGLIEALGDGTVREDVARVLKKIGDTRAVDA
                     LIGGLLGPNWMVRRHAAEALGKIGDPRSADALIQSLQDEDWLVRRNAAESLARLGAKQ
                     AIEPLLPLLEDENTMVQETVEGVLASLGWKQATSS"
     gene            8612..9322
                     /locus_tag="PK614_04745"
     CDS             8612..9322
                     /locus_tag="PK614_04745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81550.1"
                     /translation="MAEEAPVKLIQIGPKGGTKKDGFNLVTERVVAVNPEAKQLEVEL
                     LAYDGKTVVLDVGDEALEDFLKIKPGDGATIRVVEEGGKRIAKSFRIRAKDPNAAKAD
                     AMLIDLKDSHWLNRKYAAEVLGELKDPRAVLPLVEALTDEVGDVRQRAYDSLIKIGGI
                     AVASLVPLLASEEDDVRQSATEIIRKIGKPAVEPLATALADADDRLKTRIMKVLDRMG
                     YKPKAKEGAQAEPAKLLS"
     gene            complement(9344..>9758)
                     /locus_tag="PK614_04750"
     CDS             complement(9344..>9758)
                     /locus_tag="PK614_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRB81551.1"
                     /translation="VKEACGGVKGKTLGVLGLSFKPNTNDMREAPSLTILSELMKEGA
                     TVRAYDPASMEESMKLLPGMVPCQDTYDVAEGADGLIIMTEWNQFRNLDFDRLKKSMR
                     QPLLLDLRNTYESDRVVGFGFRHVSVGRPTKNPAA"
BASE COUNT         1738 a         2706 c         3292 g         2012 t
ORIGIN      
        1 taatgggtgt ggacagtttc tgattcaagg ctttccggca ttgttcgact gtggtggtcg
       61 taaagtttcc cgcgtcgccg atctggctgt cgagcagggt cttcgtggcc tcatcgttgg
      121 acagcggagg cttcatgccg cccggcagcg gggtgatgcc attcgtgcct tcccgcatga
      181 aggagaggag cgaatccagg ccggagatgc cgcgtgaata gcctcgcttg agcccgccca
      241 cttcatcgag ccgtcctaag ccgaagacat cggtgccggg gatggcgcca tgcaggaaca
      301 tgatcagccg cgcaccggct tgtgacagct tctggccgag ctggctcatc gcctcctgcc
      361 aggccggaga gtcggcgtcg ggcggcgtcg tccagtccgc gtaggtcagc cggtcgccgg
      421 gatttttgcg ggagagctcg tcgtgctgaa aattgttttc gatcggcatg ggaagattgt
      481 cttacccgaa acggagcgcg aagttcaatg gaggaacctc ggattcgacc tggaaggtgg
      541 ttcagcgatg cgctgccgac tcgcggccgt tcgattccgt ggatgctggt gcggacaggc
      601 gaggcttcgg catgacggct gttgcggagc aggtcttggc ttcttcgtcg gtgtggcgtt
      661 cgacgatccg gacaccctgc aaatattcct gaacgatttc ctctcgcacg acttcgatgt
      721 cctgctcggc ctcacgaccg gtgcgttcgc gcaggcggtc ggtggcatgt tccttcacca
      781 gcacgcggat ttgtttggca atatcggtcc tggctgccag ctcggagacc cgctgacaga
      841 cgagtttccc tttgctcagg tcgccctgtc cctggccggt cagcaactcc tctgcgctcg
      901 atgtcgcttg cgcaggtcta gcctgggctg cgctgggcgg cgattccgcc cgtccttgca
      961 tgtggagatg ttggaaggtc tggtcagcgt gatcgcgcac ggctgccggc gtcaggtctg
     1021 cagcggttgc cggcgatgcg atcagcagcg cgcagagcgg ccaccatcct gttctaatgg
     1081 gtccagccgt cagcgacatt accgcgttcc tccctgatga gttccgtttc cggggatctg
     1141 ttgcgaaggc gtcgtcggga tgggcaccgg tttcttcggt acctggtaga taatcgccgg
     1201 cggggttccg cgaggtgcat tgatcgatgg ttgttccgca gcaggagcgg cctggtcgac
     1261 tcccgatcga taggtatgga gcgtgcgcgc tgccggtcgg ccggttggcg gcgggacttg
     1321 tcgataggtc ggcatcgtgc cgcggctccc ctgcggcagc gatgctccag gctgtgccgg
     1381 tacttcaatt gcgcctggtt gagccggttg ttgcgggacg accgtgtagc ccggcaaaaa
     1441 tggaacggta tgtggcacgg gcggccgccg tggttgcggc ggtgccgggg gcgctttcgt
     1501 tcccacggtc tgctgggcga tggccaggtg gggattgtcg ggattcagcc gactcgctag
     1561 cccaaggagg gcgtgaattt gcgcgaggcc ggccgatgga gagggttgtg cgaacggagc
     1621 gagtagtacc cagtcggtcc aggcttgcgc gaccatcgca tcggactccg cgcgattgag
     1681 caactcgttc cgttcatggc tcagtccttg ggcctgttcg ggcgaggtgg cgccggcgag
     1741 ggcgtcgttg gcggcctcga gttcctgctg caacgattcc acatcccgtc tgagcgcaag
     1801 caactgctcc cgcgcgtcct cgttctgccg tagggtggca atggcctgga cgacttcgtc
     1861 ggtgtccgcc tgtgccgtca gctcgacgcg gaccacaacg aactctccat ccagggcggt
     1921 cgtcacgtgt tgatcgagga ccagcaccat tcccgcggtg taactgcgga tttcatccag
     1981 agtgacatcc agatcccgca cgaccgtgac gctttccaga tacgaggcga cctgctccaa
     2041 cgcctgcttc ttagcctgct cggtggcgag tctgatccca tccgttttag tttcgtgatc
     2101 gttcattcga tgttcgcccg ttccggtgac gactcgaacc tcagcttggc ttggactcgg
     2161 gcaggtgagg agcagggaca gggcaataga gagcaggccg ataaaaaggt ggatcgatcg
     2221 agcccgcggg gacgtgtggg ggatgaagcg ggggccgctg tcgaaccggt tttggggcat
     2281 cgcctcacca ccgcggagcg tctgactgag tgtggttgtc gagcgagttg tcccgaccgg
     2341 atcaggatac catcgatccc gcttcgacgc tatgagaggc ggggttcgcg ccttgccttc
     2401 gcggaaaaca gcatgctaag gtactttctc ttcgcttctc aaaagatcgt atggtggggc
     2461 aggttatgag tcgtattcgc agcattctga cagtcacggg agccctctgc atggtcgtcg
     2521 gtctagggct ggcactcgtg gcgccctcgt cccaatccgt tgcctttgcg gcacgcggtg
     2581 ggggggaagt ggccactcct gttcaggcag gcatcacaga acatgtgatt ctcttcgtat
     2641 tggaagggtt gggccaggaa tcgctcaaga gtggcgcgat gccggtgttg tcgtcccttg
     2701 tgaaggacgg ggccgtgacc tggtctgcta ctgcagtagc ccctgcgcgc cggttgccga
     2761 cgatggcctc gctggtgacg ggcatgccgg tcgccaaaca tgggatcacc tggaacgtct
     2821 ttgaattcag ccgtggatat cctcgcgcgc cgacggtgtt tgattatctg gacctgagcg
     2881 gcggccggga cagtgccatt ttctatatgg atgagtcgtt gtatcaactc gcgaagccgg
     2941 agccctacac ggattaccaa atgtgtggtc cgctgcgggc cgaatgtaat cctgatcgtc
     3001 tggtcggcta cgttcgggat tatttcaaaa aggcgacgag tggttcaggg tatggtcatg
     3061 ccattccagc cttgccgcat ctcctggtgg tccatctgcc ggctcccggg cgtgtcggag
     3121 agacgcaggg ctggaaatcg gttgcctaca aagacgcgtt gaaggcggtc gataaggcga
     3181 tggggaccgt gctcgatctg taccgggaac atggtttgat caagcggacc acggtgtttg
     3241 ccacctcgct gagtgccttc ggggagacgc aattctcggc cggtgaggtc tccggtgagc
     3301 aggccggcaa tgttccggtg gtgccatgga ttgcctcagg cgtcggcatc aaggctgggc
     3361 atacgattcg tcagccggtg tcgattatcg atacgggcgc gacggtgatg cgcgcgctcg
     3421 gactcacgac gtataccgaa tgggagagcc acccggtcga agaaatcttc aagaccgcat
     3481 tcgctgctgc tcccgtcagc ccactcctgc aatagaggac cgatgcgttc atcatcgcac
     3541 aaagcattcg ccgggcggca ggtgtttttc tggatcgtag ccgtgacgtg tgtgttggtt
     3601 ctagggccat ccgagcggct cttggcggcc ggtcctcccc ctgcctatgg caaagaacat
     3661 acgattacaa tcgatccgac tccattggtg ccgcaatcct ggtggcaggt gcccggtgtg
     3721 acacccccgc tctgggtctt ggacccggag acctcggatg cctaccggac aactgaaacg
     3781 cgcgagctga agctcaagcc gggacaatac aagttcatca gttttacctt cgacttcccc
     3841 ttcacggtga gtttggacgg gaaactggaa tactccaagt cactcgatca atgtgtggag
     3901 ggccgcggga ctcagaccct ggtggtacgg tgcaagcgca cctatcctca tggcggtcaa
     3961 cgtgatgcct attacgggaa tagtgaggtg ggtgatggca caggcactcg atgatctgct
     4021 ggattccctc gaagatgcgg acgacgccac gcgtgaagag gcggcgaaag ccctcgccga
     4081 gttagcgaac ccggccacgc tggatgcgct catcggcgcc tgcggcgann nnnnnnnntt
     4141 tggtctgtgc ggactcgtgc cggctggggc gtggctaaga tcggcggacc gaaggcgatc
     4201 gaagcgctga tcgtgctctt caacgacccg atcatggaag tccgcaacga agcggttgcg
     4261 gccgtggtct cgcttggtgc gggccagctg gatcggctgc ttgccgcgat gaaggatgag
     4321 cggtggcggg ttcgggagca tgcggccaaa gcctgcgggg atctccatga ttcgcgcgcc
     4381 gtcgatgcgt tgacatttgc ctgtcgggat cgcgacgggg ctgtgaagag tgccgccgcg
     4441 gaagcgctgg gaaaaatcgg cgatccgaaa gccattcccg cgctggtgaa attattccgt
     4501 gattcatcca agatcgtccg tgaaaccgcc ggtattgccc tggtcgcgat cggacagccc
     4561 tccgtcgatt tgttgctcga gacgttgaaa gacaaagatt tcgtcgtgcg ttgtcatgcg
     4621 gctcgcgcat tgggaggcat gacgacggac tatcaaatcg gccggagttg ggtgcgggag
     4681 ccgcgcgtcg tcgatgcctt gatcgaggcg ctgaaagatc cggaccgggc cgtgcgggaa
     4741 gatgccacga ttgcattggg catgatcggc gatccccgcg ccatcgacgg gctattggaa
     4801 gcgatgaaag acggtgccgt gaagcgccat gccatcgctt ctctgggtat gatcggcgat
     4861 cctcgagccc ttcctgccgt cttggctgcg ctcaagggaa agggtgtgcg tcaggatggc
     4921 actccgacgc ccgggtgcat cgtcagtgaa gacgccttta tcaaggaagc agcggccacg
     4981 gcgctcggcc acttccgcga tccgcgcgtg attcccgatc tgatcatgct gttgaaagac
     5041 ggggtgttgc gtgagaaagc ggcggcggcg ctggtgctga tcggtgactc ggctatcgaa
     5101 ccgctcatct cgtttctcta tgatcccaaa gcgtcggaag ttgaagccga gggcgaacgc
     5161 gtcctgtcct acgcctcggt gcggctgacg gccaaagatt ccctgcggct acttgttgtt
     5221 gaaacactgg aacatcttgg gtggacgcct ccggatgaag agacgtcggt ggactccagt
     5281 caggccgaca atcttcgtgt cgaccgcccg ctgggcgaca tcggacggtt cggcccgagc
     5341 ggagattttg caaaaggatc ggtgcggtaa tcaccggctt cctccccagg ggtccgtccc
     5401 cagggatgag catctccggc gctcctaccg gctactctcc tctgtttaga ccagatgcgc
     5461 gggaacggtt cgatgccagg tcgcacggcc gaccctgcgt tgacccctca aaaaatcctg
     5521 tgttaaggtc gatggcctca ccaggggccg aggtaccatg gccgacactg tcagtgaaca
     5581 gatcgcagcg ctgtcggacg aagattgggc gattcgggaa gaagctgcga ccttgctggg
     5641 cagcctcaac gacgcccggg cagttctgcc gctgacgaag ttgctccgcg ataccgatcg
     5701 agctgtccgt gaggcggcga tcgggtcgct ctccgcgctc ggctccgcgt cggttcccgc
     5761 tctggccggc tgcttggcgg atccggcgtt gcaggtgcag gaagcggctt cggccatttt
     5821 ggcctcgatt gccgatgcgc gggttttccg cccgctggtc gaggccctgg gcagtcgcga
     5881 ttggatcgtg cgcatgcatg cggcgaaggg gctgggacgg atcggcgatc ccgaagccgt
     5941 cagcgtcttg atgccgctct tgcaggacaa ggtcaaggcg gtgcgtgagg aggcctccag
     6001 cgcactggcc gccatcggtg ctgccgctgt gttggggttg atccaagctc tggaacacga
     6061 tgactggttg gtccgcctcc atgccgtgga ggcgttgggt aagctgaagt ctccggattc
     6121 tgtcgatccg ttgctgcgtg ccctgttcaa tgagcgggac tcggcgattc gcgaagatgt
     6181 ggtgcgggcc ttgggtgcga tccgggatgc gcgggcggta gattatctgg tgacggtgat
     6241 gaaggaaccg gggttgcggc tgctggccgt cgaggcgctc ggccatatcg gcgaccctcg
     6301 tgccgtgcct cttcttcggc gggttgtgga gggggtaccg ctgggcgagc ctcgcgatac
     6361 ggccacccct tgtgccgacg ggtggacgga cgagatggcg accatgggga tggcggctcg
     6421 cgcgttgggg atgatcgccg acggcgtggc tattccatct ctgatcgtgg ccttgcggaa
     6481 taccattacc cggtctgagg cggctgcggc tttggccaag ttcggcccgg cggtgattcc
     6541 gtccctgctc ccgatgctgg caaaggaaca ggacgagaat attcggtatc acgttcgaga
     6601 gacgttgacg gctgtcggct ggcgtccggg acgggtctga cagggtatta gttcgtgatg
     6661 tggtgaggta gccacggctg atcttcgcct ctggcactca ccgaatcaga aaacttcgca
     6721 ctcgccaatg aacacatgtc taaagaaagc atcgatacac tcgtttctga gctgacccac
     6781 gaggaagagt ggcggcgtat gcgggccaca gctgcctgct tggccggtgg gcctcgggcg
     6841 gtgcaggcgt tgacgcaggc cttggaatcg ggcgatgttg ctctccgcgt tgaagcggcg
     6901 gcgatgttgt cgcggatcaa ggatccggca gccggacttt ctttggtcgg tttacttcgg
     6961 gattccgagg aaaccgtccg gcaagccgct ttttcagcgc tggaacaaat ggccggcaat
     7021 ctggatgacg agacagccgc gggtctggtg cgcaatctcc acgagtctcc ggatgaggat
     7081 gtgcgaaacc gggttcgtca gctcctgggt gtgatcccca acgccatcac tcccttgtgc
     7141 gaaatgttga cccatccgga ggtcgacgcg cagacgaccg cggcgacgat cctcgaacac
     7201 ttgcttgatc cgcgctcggc ggacggattg atcgatgcca tgtcctctcc cgcggttcgt
     7261 gacaccgccg tgcgcacgtt gaagaagctg ggggcgatac gggaccggat cgatgcgacg
     7321 tttaatgcct tgcgtgacgt ggagggggcg agcgagcgtg aagaagcccg catgtccacg
     7381 gtcatggatt tactggggat cgggcgtccc gcggtcgaaa ttttgatcga gtatctggaa
     7441 gatgacgact gggtagtgcg tgaagcggcc gcggacttgc tggggaagat cggggatgtg
     7501 cgcgcggtcg agccgttgat gaagcggctt cagttggata aggataccgg agtgaaggag
     7561 tatgccgtga agtcgctggg gctcatcgga gacccgcgtc cggcgcaatt gtatatcgag
     7621 gcgatcccga tcaggccctt gcgggtgatg gcgatcgagg ccttggcgaa aatcaaggac
     7681 gtcgaggtgt tgcgtccgta taacgagctg ttcaaccggt tgcggaccga ccgggacggc
     7741 atcgtgtcgt acagcgcggg ccttatcgcg gacaaacttg ccgcgcttga aggcgaacag
     7801 ccggtcggac aggaggaatc ggacgatcat gagtgatgcc gagcgaattg cacaattaat
     7861 ctccgcgcta cgcgatgaca acgaggcgtt gcgggaccat gcgatggcga gcctcgggca
     7921 aatgggcatc gaggccgttc cacagctgat cggcctgatg gccgatgaag acgtggtgat
     7981 tcgcgaagcg gccgcgacgg ctgtggtgcg aatcgggccc gtggcgttcg accagatgct
     8041 ggaggcgctg agtgacgatg agtgggcgat tcgcgagcag gcggcgaatg tgctcggacg
     8101 tttccgcgat ccgcgtgcgg tggatcccct catggtggcc ctgaaagaca aggacggagc
     8161 cgtccggacg gcggccgtct gggctctgga acggatcggc gactcccgcg cgacgccggg
     8221 attgatcgag gcgctcggcg acgggacggt gcgagaggac gtggcgaggg tcttgaaaaa
     8281 gatcggtgat accagggccg tcgatgcgct gatcggcgga ctactgggcc ccaattggat
     8341 ggtgcggcgc catgccgcgg aagcactcgg caagatcggt gatccacgca gcgccgatgc
     8401 gttgattcag tcgctccagg atgaggactg gctcgtgcgt cggaatgccg ccgaatcgtt
     8461 ggcgcgcttg ggggcgaagc aggccatcga accgctgctc cccctcctgg aagacgaaaa
     8521 cacgatggtg caggagaccg tcgaaggtgt ccttgcgagt ttgggatgga aacaggcgac
     8581 ctcgtcataa cccgataaag gaactctact tatggcagaa gaagcaccgg taaaactgat
     8641 tcagatcgga ccaaagggcg ggaccaagaa ggacgggttc aacctggtga cggaacgcgt
     8701 ggtggccgtc aatcccgaag ccaagcagtt ggaggtggag ctgcttgcgt acgacggaaa
     8761 gaccgttgtg ttggatgtcg gcgatgaggc cctggaagat ttcctcaaga tcaagcccgg
     8821 cgatggggcg acgatccgtg tggttgagga gggcggcaag cgcattgcaa agagcttccg
     8881 gattcgcgcc aaagacccga atgcggccaa agccgatgcc atgttgatcg acctcaagga
     8941 ttcccattgg ctcaatcgca aatatgcggc ggaagtgctg ggtgagttga aagatccgcg
     9001 cgccgttctg ccgctcgtcg aggccctgac cgacgaggtg ggcgatgtgc ggcagcgagc
     9061 ctatgattct ttgatcaaga tcggcgggat cgccgtcgcg tcactcgtac cgttgttggc
     9121 ttctgaggaa gacgacgtgc gccagtcggc gacggaaatt atccggaaga tcggtaagcc
     9181 ggccgtcgag cccctcgcca cggccctggc ggatgccgat gatcggctga aaaccagaat
     9241 catgaaggtg ctcgatcgga tgggctataa gccgaaggcc aaagagggtg ctcaggccga
     9301 gcctgccaag ctgctcagtt agtgtgagcc gtccggccgc cggttaggcg gccggattct
     9361 tagtgggacg accgaccgag acgtggcgga atccgaagcc gaccacgcgg tctgattcat
     9421 aggtattgcg gagatccagc aggagtggtt gccgcatgga tttcttcaac cgatcgaaat
     9481 caagattcct gaactggttc cactcggtca tgatgatcaa gccgtcggct ccttctgcga
     9541 cgtcgtaggt gtcctggcag ggtaccatcc ccggcaaaag tttcatcgac tcttccatcg
     9601 aggccgggtc gtaggcgcga accgtcgccc cttccttcat cagttcgctc aagatggtca
     9661 gtgacggggc ttcccgcata tcattggtgt tgggtttgaa cgacaggccc aacacgccga
     9721 gcgtttttcc cttcacgccg ccgcaggcct ctttcacc
//
LOCUS       DAORQN010000050         9638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_380_length_9638_cov_134.911823, whole genome shotgun sequence.
ACCESSION   DAORQN010000050 DAORQN010000000
VERSION     DAORQN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_9638_cov_134.911823"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK614_04755"
     CDS             complement(<1..336)
                     /locus_tag="PK614_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81552.1"
                     /translation="MPTPPHITIVLVVSRIDEKRLRETIESLIAQTYPNWTLSVVGEV
                     SRLRMMEHIINAYSEADSRIQPEEISEKERISDRILKGRLGEFVSFLSLECYLAPEAM
                     SSVITRFQEC"
     gene            complement(779..2065)
                     /locus_tag="PK614_04760"
     CDS             complement(779..2065)
                     /locus_tag="PK614_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HRB81553.1"
                     /translation="MAGICFNGDNHPGPRLIAFFLTQFHPIPENDEWWGKGFTEWTNV
                     TKASPLFDGHYQPHLPTDLGFYDLRLRESRHEQIRLAKQYGIDGFCYHYYWFSGTRLL
                     NRPLDDMLVDRESDMPFCLCWANENWTRRWDAAEHQVLISQKYLPDDDLNFIKSLVPF
                     FKDPRYIKLRGEPFLIVYRPQHLPNPRSTVQVWRDYCETVGIGKIHICAALTHGNEQY
                     GQFGFDSGVEFPPHNLNCRNVNDRIEFHDQFYGNAMEYQDIAQTYLTRQYRESNVFKT
                     VFPSWDNTARSRERALVVLNGTPHNYEHWLAKTLDQTVNTYPNEDNLVFINAWNEWAE
                     GCHLEPDRRFKLEFLEATLRAKNNLSTIQGFQSLRAFAGDGVVRRSIYGDLLSIARYH
                     GWRTITTWRYWINQYPRLKDFIKMLISPIRRLIANG"
     gene            complement(2120..2965)
                     /locus_tag="PK614_04765"
     CDS             complement(2120..2965)
                     /locus_tag="PK614_04765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB81554.1"
                     /translation="MSTKPRHKYEFSVDLTAPSAPTYVVRMVGHFKRVLEVGCGPGSV
                     TKLLTQHGQCRVTGLDLDSEAIDRVAPYCEAIVRGDLNSPEWPSLLDKVKPFDVVVAA
                     DVLEHLYDPWSVLAQMAGFIRPDGYLVISLPHVGHAAVASCLINGDFEYREWGLLDRT
                     HIRFFGLKNIQDLFTQAGLKILEAEYVVKTPEETEFAASWCRLSAAVQDALKSSDYAD
                     IYQVVVKAVPISHPGYAVSLVPPEPRHTAVGMRTLWKTRLAHHLSQSTRQRIRQGLSL
                     LGIRL"
     gene            complement(2925..4121)
                     /locus_tag="PK614_04770"
     CDS             complement(2925..4121)
                     /locus_tag="PK614_04770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB81555.1"
                     /translation="MGQFNSTLVQDGRLLPGIHGLRGIAAIAVVFFHLVHVGGLNPPT
                     SFQFIGRDFGYGVHLFFILSAFSLMHSTESRTTAPSWLSDYFVKRFFRIAPLFYLMIV
                     FWVVVGVVKGAGSKDPAEILLNLTFTFGFVPPSGIVWGGWSVGVEVIFYAIFPVLLLL
                     VKPHKPSLLLLLLLVVSIIVSGALRSVLHLQGSNVESSSKFDWSYFSFPSNICFFLMG
                     LYVYLVVKFHGRHSTLLRVHAPIATTAIIAGLLFSDVGLLIYSSARWDIVLWGFGLSA
                     LCAWQANRPSRVIASGVLQYFGERSYSIYLMHPVIIFALKSYLLKIYTLCFPAIGAYS
                     YFICSSILIVSILPCVEITYRLIEVPGINLGRRLIASKRIAVITPTLDLESHEYQASS
                     QVRILS"
     gene            complement(4151..4927)
                     /locus_tag="PK614_04775"
     CDS             complement(4151..4927)
                     /locus_tag="PK614_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81556.1"
                     /translation="MSSPILVWENDSNLRVNGVDFYVAYSPEELHGINSTVDKFLLGK
                     SRSMIEKLMTVLAGQKICNVFDIGIFKGGSVALYDQLFPTEKIVAVEFMSRPVDALTQ
                     YIHRHKKADHIKPYYGVNQADRRAMRKILAYEFPNQEIDLIIDDASHLYEETREAFNI
                     SFPYLKPGGHYVIEDWAWAHWPGDYWQKDNPHFKDKRALSNLLIELTMLAASRCDLIE
                     DIFVNHNYVIVKRGVSKLSPDGFDIGEHYLLRGKQFGAWL"
     gene            complement(4924..6054)
                     /locus_tag="PK614_04780"
     CDS             complement(4924..6054)
                     /locus_tag="PK614_04780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRB81557.1"
                     /translation="MNAELVGSQDKLMRVRNEYLDLMQAFLTGSIYRDFAQAPFGSNL
                     FDSHRREHGLDWPSTAETMIGVKRLANLRALTESVIADNVPGDLIETGVWRGGACIFM
                     RAILYANSVSDKSVWVADSFEGLPAGNTLQYPADAGSDFHTYSQLAVSLEEVKENFRA
                     HGLLDAQVKFVKGWFKDSLPKAPIDRLALLRLDGDMYESTMDALTYLYPKLSRLGYVI
                     VDDYHVVPACKAAVSDYCEKNAIRPQIVEIDGVGVYWRKSSVPEGQSEIVSTNEVVAS
                     FDVQIARLDQAVLELSKRAIAQLNRCLSERDELIASMTQSLAEQDRQIVNLKQTLVQR
                     DEYIAALLSSASWRVTEPLRVMGALLRKFHPLGHTNLKGREK"
     gene            complement(6116..7381)
                     /locus_tag="PK614_04785"
     CDS             complement(6116..7381)
                     /locus_tag="PK614_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81558.1"
                     /translation="MPYFREFWALKNCSLEIRKGETVGIVGGNGSGKSTLLQLICGTL
                     MPTNGGVEVHGRIAALLELGAGFHPEFTGRENVYMNASILGLSQTEIDAKYDEIVAFA
                     EIGDFIDQPVKTYSSGMFVRLAFAVIAHVDAEILVIDEALAVGDVFFAQKCMRFLRTF
                     KSYGTIVLVTHDFGTVVGLCDRAIWLEQGEVRSIGQAKQVCEEYLANRYGAVRSTIPR
                     QVPEVSMSNAEQRSLSKHDARIDFINHSNLRNDIEVVDLSAERHGFGNGGASITDVHV
                     TDLEGRQLSWVVGGEMVRLEIEALVTVPCKNTIIGFQFKDRLGQVLFAQNSFTAYCMN
                     PVSASPNDTVKGVFVFRLPILPRGKYSVDVAIADGASPESVTQLQWLHDAFSLESHTS
                     SVVSGLVGLVFDSIELSSQGTPFSKTAVA"
     gene            complement(7497..8321)
                     /locus_tag="PK614_04790"
     CDS             complement(7497..8321)
                     /locus_tag="PK614_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB81559.1"
                     /translation="MGSQRYSLSPLMVFTSLYSHWNLIRLLTGREIASRYRGSLLGVL
                     WSLAHPIMMLVVYAFVFRVVFKASWSHGGEGNSEFVMTLFTGLIVCAVFNECVNRAPT
                     LILNHVSYVKKIVFPLEILPWVSLGEAFLNMGVSITVLLVFHISSHLPLHWTATMLPL
                     LVIPLALYALGVSWFLASVGVFVRDVGHATTIVTNLMLFLAPVFYPLSSLPDAYRPFM
                     YLNPLTFVVEQSRNVLIMGRMPSWHWVVIYFLCSAVVAWLGLAWFQRTRKGFADVL"
     gene            complement(8444..9208)
                     /locus_tag="PK614_04795"
     CDS             complement(8444..9208)
                     /locus_tag="PK614_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRB81560.1"
                     /translation="MGAIGVIALNAFRESLRDKILYNLVLFAGLLIGLSVLLADLSIT
                     EHHKVIVDMGLAAINLIGVIIAIFVGISLVNKEIERRTIYTIMARPISRASFIMGKYL
                     GLALTLFVNMAIMWAVFLLTLWLYHVSIQPSIFQAVALIFVEILVVTAIALFFSTFTS
                     TTLSAIFTLGLYVIGHLTADLRAMVANSDSGPIKSLVDLLYYLCPNLEMLNIKGQAAV
                     GVVAAPEYVMLASLYGVMYAGVLLISACVVFQQRDF"
     gene            complement(9208..>9638)
                     /locus_tag="PK614_04800"
     CDS             complement(9208..>9638)
                     /locus_tag="PK614_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81561.1"
                     /translation="ILNLRDRGKTVFFSTHILHDVEMICDRVGIVMKGRLVASGRVDE
                     LVRQDHTQSVEVVCQQLKVEENAFIHSLATRVLQQGQQCLIVLPSPDAVDALVGEIRR
                     QGGRLLSVTPHKASLEDLFFQEATQRTSPSLPHVTSGRAS"
BASE COUNT         2636 a         2557 c         2223 g         2222 t
ORIGIN      
        1 acattcttgg aagcgcgtga tcacagagga catggcctct ggggcaagat agcattccag
       61 agagagaaaa cttacgaact ctcctaagcg ccctttgagt atccgatcag atatgcgctc
      121 cttctccgag atctcttcag gttgaattct ggagtccgct tcggaatagg cgtttatgat
      181 atgttccatc atgcgcagtc gagaaacctc accgaccacc gacaacgtcc aattgggata
      241 cgtctgggca atcagtgatt cgatagtctc tcgcaatctc ttctcatcaa ttcgactcac
      301 taccagcacg atcgtaatat gcggtggtgt cggcatttgc cctgccgcaa gacgcatccg
      361 ctgtaagcat tctcgagata gtgtgtggcg cttccgccat acctcatact gggctgactg
      421 gctttggagt gtaaaaggaa gccccgaaga atctccgaac gcaatgctgc gtactttgaa
      481 taaacctttc cgtaggcatg ctccaatccc ttcattcgcg agcacttccc agaaacggga
      541 tcgtagccaa tacagatcag acaatatatt catctgaaac aaatgatcat tcgggcgtaa
      601 tcatctctct cgactatgag ttttcgcgca cgatctatgg gccagagtcg ggaccaggaa
      661 aactctgccg tcggaaggac cggatcgtct tatcggcagc atcatggccg acaagttact
      721 ggtaaataca ctctctaggc acaaagaatg cgtatcggcg cctaccactc atcaccatct
      781 aaccgttcgc gatgagtcgt ctgatagggg aaatgagcat tttgataaag tccttgagtc
      841 gaggatattg attgatccaa taccgccatg tcgtgatcgt acgccagccg tgatagcgag
      901 cgatcgagag taaatcgccg taaatggatc ggcgaacgac gccgtccccg gcaaacgccc
      961 tcaatgactg aaatccttgt atagttgaaa gattattctt agcccgcaat gtcgcctcaa
     1021 gaaattccag cttgaaacgc ctgtctggtt ccaagtgaca cccttcggcc cattcgttcc
     1081 atgcattaat aaagaccaag ttgtcctcgt tgggataggt attcaccgtc tgatccaatg
     1141 tcttggccag ccaatgctca taattatgcg gcgttccatt gagcacgact aacgcgcgtt
     1201 cccgcgaacg agccgtattg tcccaggatg ggaatacggt cttaaagacg ttcgattcac
     1261 ggtattgacg agtcaaatac gtttgagcaa tgtcctggta ctccatagca ttgccataaa
     1321 actgatcgtg gaattcgatg cggtcgttga cgttccgaca gttaagattg tgaggaggaa
     1381 actcgacgcc actatcaaac ccaaactggc catactgttc attgccatga gtcagcgctg
     1441 cgcaaatatg aatctttccg attcccactg tctcacaata atctcgccac acctggaccg
     1501 tgctgcgggg gttcggcaaa tgttgcggtc ggtatacaat taagaacggc tctcccctaa
     1561 gcttgatata ccgtgggtcc ttaaagaatg ggaccagact tttaataaaa ttcaagtcgt
     1621 catccggtag atacttctga ctgataagaa cttgatgctc agccgcgtcc catcgccttg
     1681 tccaattctc atttgcccaa cacagacaaa acggcatatc actttctcga tcaaccaaca
     1741 tatcgtccag cggtcgattc aataaacgtg tgccggagaa ccagtaatag tgatagcaaa
     1801 acccatcaat gccgtattgc ttggcaaggc gaatctgttc atgccgactc tctcgcaaac
     1861 ggagatcata aaatcccaaa tcggtcggta gatgaggttg atagtgaccg tcgaacaatg
     1921 gggacgcttt cgttacattg gtccattccg tgaatccttt tccccaccac tcgtcatttt
     1981 caggtatggg atgaaattgt gttaaaaaaa aggcaatcaa cctgggccca ggatgattgt
     2041 ctccgttgaa gcaaatgccg gccattcctt actcccaatc gctgtcaggc aagctgcaga
     2101 ggcacggcgt ttgttgcgat catagtcgga taccaagaag actcagaccc tgccggatcc
     2161 gctgtcgagt gctctggctt agatgatgag cgaggcgtgt cttccacaat gtccgcatgc
     2221 caactgcagt atgacgtggc tccggaggca ccaacgagac ggcatatcca ggatggctga
     2281 tgggaacagc cttgacgacc acttgataga tatcggcgta atcactactc ttcaaggcat
     2341 cctgcacggc ggcagaaagt cggcaccaac tcgcggcgaa ttcggtttct tccggagtct
     2401 tgacgacata ttcggcctca aggatcttga ggccggcctg cgtgaacaag tcttggatat
     2461 ttttgagacc gaagaagcgg atatgcgtac gatcgagcaa cccccattca cgatattcaa
     2521 aatcaccgtt gatcaagcac gacgcaactg cggcatggcc gacatggggc agtgagatga
     2581 cgagataacc atcaggtcgg ataaagcctg ccatctgtgc cagaacgctc cagggatcgt
     2641 acagatgttc gagtacatcc gctgccacca caacatcaaa tggtttcact ttatcgagca
     2701 agctcggcca ctcaggggaa ttcaaatccc ctcgcactat ggcttcgcaa tatggcgcga
     2761 cgcggtcgat tgcttccgaa tccagatcaa ggcctgtcac tcgacattgt ccatgctgcg
     2821 tcagcagctt ggtgacggaa cctggcccac agccgacctc caggacacgc ttaaaatggc
     2881 caaccatccg taccacataa gtcggcgcgg atggtgcagt gagatcaact gagaattcgt
     2941 acttgtgacg aggcttggta ctcatggctc tccaaatcga gagtaggtgt gatgacagca
     3001 atcctctttg aggcgatcag tcgccggcca agatttatcc caggcacttc tatcagccta
     3061 taagtaattt caacgcaagg caaaatagaa actataagaa tgctcgaaca tatgaagtag
     3121 gagtaggctc caatggcggg aaagcacaaa gtatatatct tcagcaaata gctctttagc
     3181 gcaaaaataa ttaccggatg catcagatag atgctatagc tgcgctcgcc aaagtattga
     3241 aggaccccgc tggcaatgac acgacttggt cgattcgctt gccaagcgca taatgcgctc
     3301 agtccgaatc cccacagcac aatatcccat ctcgccgaac tgtaaattag caatcccaca
     3361 tcagaaaaaa gtaatcctgc gataatcgca gtcgttgcta tcggtgcgtg cacacgcagc
     3421 aatgtcgaat gccttccgtg aaatttgacc accagataca cgtagaggcc catgaggaaa
     3481 aagcaaatgt tggaagggaa ggagaagtag ctccagtcaa atttcgatga tgattccaca
     3541 tttgagcctt gaaggtgcag gactgaacgc agtgcgccgc tgacaatgat gctgacgact
     3601 aatagtagga gaagtagaag agatggctta tggggtttga cgagcagcag taagaccgga
     3661 aatattgcgt aaaatatcac ttctaccccc acagaccacc ccccccaaac aatgccggag
     3721 ggtggcacaa aaccaaatgt gaaggttaga ttcagtagaa tttctgcagg gtcttttgat
     3781 ccggctcctt tgacgacccc aacgacaacc cagaagacaa tcatgaggta aaatagggga
     3841 gcaatcctga aaaatctttt tacaaaataa tcacttagcc aacttggcgc ggtcgtccgc
     3901 gattcggttg aatgcatcaa cgagaatgcg ctgagtatga aaaaaagatg tactccatag
     3961 ccgaaatcgc gacctatgaa ttggaatgaa gttggagggt ttaagcctcc cacgtggact
     4021 aggtgaaaaa acacaacggc aatagcggca attcctctga gaccgtggat gccgggtagc
     4081 agccgtccgt cctgcacaag ggttgaattg aattggccca tggttacagc tttataggat
     4141 caagcggttc ctatagccag gctccgaact gcttgcctcg caacaaatag tgttcaccaa
     4201 tatcaaagcc atccggagac agcttgctga ccccccgttt gacgatgacg taattgtgat
     4261 tgacgaaaat atcttcgatc aaatcgcacc tactggcggc gagcatggtt agctcgatta
     4321 gcagattgct caaggcacgc ttgtccttga aatgaggatt gtctttttgc caatagtctc
     4381 caggccaatg agcccaggcc cagtcctcaa tgacatagtg cccacctggc ttcaggtatg
     4441 gaaagctaat gttaaatgct tcacgggttt cttcgtacag atgggacgca tcatcaatta
     4501 tcaagtcgat ctcttggttg ggaaactcgt acgccaaaat ctttcgcatc gcacgccgat
     4561 ctgcttggtt cacgccataa tacggtttga tatggtccgc ttttttatgt cgatgaatat
     4621 attgcgtgag tgcatccacc ggtcttgaca tgaattcgac ggctacgatc ttttccgtcg
     4681 gaaaaagttg atcatacagc gctacacttc cgcccttgaa tatgccaata tcaaaaacgt
     4741 tgcaaatctt ttgtcccgca agaacagtca tcaatttttc gatcatggac cggctcttgc
     4801 ctaggagaaa tttgtctacc gtggaattta ttccatgtag ttcttcaggg ctgtaagcaa
     4861 cataaaaatc cacaccattt actcttaaat tcgagtcatt ctcccaaact aaaattggcg
     4921 agctcatttt tcccttccct taagattagt gtggcctaac gggtggaact ttctaagtaa
     4981 agcacccatt acccgcagtg gctcggtcac acgccaacta gcagatgata aaagtgcagc
     5041 gatgtactcg tcccgctgaa ctagcgtctg cttgagattc acaatctgcc tgtcctgctc
     5101 cgccaaggat tgggtcattg aagcaattag ctcatcccgc tccgacaaac accgattgag
     5161 ctgagcaatt gctcttttgc tcaattcgag caccgcctga tcgagtcgag caatctgcac
     5221 atcaaaggac gccacgactt cgtttgtcga aacaatctct gactgcccct cgggcacaga
     5281 agacttcctc caatagactc cgacaccatc aatttccaca atttgcggcc ttatcgcgtt
     5341 tttttcacaa tagtcactta cagccgcttt acaggctggt accacatggt agtcgtcgac
     5401 aatcacgtac cctaggcgtg agagctttgg atacaggtaa gtcaatgcgt ccatagttga
     5461 ctcatacatg tcgccgtcta atcgcaacag tgccaatcgg tctatcggcg cttttggcaa
     5521 actatccttg aaccagccct tcacaaattt aacctgcgca tctaatagcc cgtgagcacg
     5581 gaagttttct ttgacttcct ccaaagacac cgccagttgg ctataagtat gaaaatcgga
     5641 cccagcatcc gctgggtatt gaagcgtgtt gccggccggc aagccttcaa acgaatcagc
     5701 aacccacacg gacttatcac tcacgctgtt agcatacaga atggctcgca taaagataca
     5761 cgcaccgccg cgccacaccc ctgtttcgat aaggtcgccg ggcacattgt cggcaatcac
     5821 ggattccgtc agtgcccgca ggtttgcaag gcgttttaca ccgatcatcg tctctgcagt
     5881 tgaaggccaa tccaggccat gctctcgacg atgcgaatca aacagatttg atccaaaagg
     5941 tgcctgagca aaatctctgt aaatactgcc tgtcaaaaac gcttgcatga ggtcaagata
     6001 ctcgtttcgc acacgcatca atttatcttg agagcctacc aattccgcgt tcatatgttt
     6061 actcactggc taataaccac tcagatagtt catagacaag tttcgcacgc cagtttcagg
     6121 ctaccgccgt tttcgagaat ggcgtcccct gactgctgag ctcaatcgaa tcaaaaacca
     6181 aaccgaccaa accactgacc acgcttgacg tgtgagattc cagactgaat gcatcatgta
     6241 gccattgcag ttgggtgaca ctttcgggag aagcgccgtc cgcaattgcc acatccaccg
     6301 aatacttgcc gcgtggcaat atcggcaaac gaaaaacaaa aactccctta acagtatcat
     6361 tcggacttgc tgaaactgga ttcatgcaat atgcggtaaa ggaattttgg gcaaacaata
     6421 cttgaccaag cctatcctta aactgaaacc cgataattgt gttcttacaa ggcactgtga
     6481 ctagagcttc tatttccaac cgtaccatct caccaccaac gacccaagag agttgcctgc
     6541 cttctaaatc tgtcacatgg acatctgtga tcgaggcgcc tccatttcca aaaccatgtc
     6601 tttccgcgga aaggtccacc acctcaatgt catttcgtaa atttgagtga ttgatgaaat
     6661 caatgcgcgc atcatgcttt gacaacgagc gttgttctgc atttgacata gacacttccg
     6721 ggacctgcct gggaatggta ctgcgcacag caccataacg attcgcaaga tattcttcgc
     6781 atacctgctt tgcttgacca atcgaccgta cctcaccctg ctccaaccat attgcacgat
     6841 cacagaggcc gacaaccgtt ccgaagtcat gtgtcacaag tacaattgtg ccgtagcttt
     6901 taaaagtgcg caaaaatcgc atgcatttct gcgcaaaaaa aacatctccc accgccaatg
     6961 cctcatcaat aaccaagatt tccgcatcaa catgcgcaat gacggcaaag gccaggcgga
     7021 caaacatgcc actggagtat gtcttaacag gttgatcgat aaagtcaccg atttcagcaa
     7081 aagcaacaat ctcgtcatac ttagcatcaa tttccgtttg agatagtcct agtatcgaag
     7141 cgttcatata gacattctct cgcccggtaa attcggggtg aaatcctgca ccaagctcaa
     7201 gaagggctgc gatgcgtcca tgaacttcaa cacctccatt cgttggcata agtgtcccac
     7261 agattagttg gagtagagtt gattttcctg atccgttgcc tccgacgatt ccgacagttt
     7321 cgcccttcct aatctctagt gagcagttct tgagcgccca aaactctcga aagtagggca
     7381 ttgaagaaat ccccaacact cttcgcaacc tcgggataaa ggactgcttc aaccgatcaa
     7441 caggcctgtg atagaggtga taacatttac tgagatcttc tactctgatc gcgatgttag
     7501 aggacatccg cgaacccctt tcgtgtacgc tgaaaccaag ctagtcccaa ccatgccact
     7561 acagcgctac acagaaagta gatcactacc caatgccacg aaggcattct tcccataatc
     7621 aatacattcc gcgattgctc cacgacgaat gtgagtggat tcagatacat aaacggacga
     7681 taggcatctg gaagcgaaga aagcggataa aacacagggg caagaaatag catgagattg
     7741 gtgacgatgg tagtggcatg tcccacatcg cgcacgaaca ccccgactga ggctagaaac
     7801 cacgaaacgc cgagcgcata caatgccaac ggaatgacta agagaggcaa catagtggcc
     7861 gtccaatgca gagggaggtg gcttgaaata tggaacacga gaagcacggt tatgcttacg
     7921 cccatgttaa gaaatgcttc tcctaacgac acccacggga gaatctccag agggaatacg
     7981 atcttcttta catagctgac gtgattcaga ataagggtgg gagcacgatt gacacattca
     8041 ttaaataccg cgcacacgat caatcccgta aagagggtca tcacaaattc tgaattaccc
     8101 tcgcccccat ggctccatga cgccttaaac acaactcgaa acacaaatgc gtagaccacc
     8161 agcatcatta ttgggtgcgc tagtgaccag agcaccccta ataatgaccc tcgataccga
     8221 ctcgcaattt cgcgcccagt caacagtcga atcagattcc aatgactgta cagactggtg
     8281 aataccatga ggggggagag actataacgc tgagatccca ttggcggcct ttgctacgta
     8341 aactgcggct cgatcatcaa tcgagcgggg atgatcgtat caagtcttct tgcggcgagc
     8401 ggcacgtccc ttccttggga gctagacaca gcacgacgtt ggatcagaaa tcccgctgct
     8461 gaaacactac gcaagcactg atgagtaaaa ccccggcgta catgaccccg tagagagacg
     8521 ccaacatgac atactccggc gcggcaacaa cccccactgc agcctgcccc ttgatgttca
     8581 gcatttcgag gtttgggcag agatagtaga gcaggtctac gagagatttg atcgggccgc
     8641 tgtcgctatt ggcgaccatt gcccgtaggt cggccgtcag atggccgatc acatacaggc
     8701 ccagcgtgaa gatggcgctc aacgtcgtcg atgtgaacgt cgaaaaaaag agggcaatgg
     8761 ccgtcaccac caaaatttcc acgaagatca gtgccaccgc ttgaaaaatg gatggctgaa
     8821 tcgacacatg gtagagccac agggtcagaa gaaacaccgc ccacatgatg gccatgttca
     8881 cgaacaacgt cagcgccagg ccaaggtact tgcccatgat aaaagaggca cgactgatcg
     8941 gcctcgccat aatcgtgtag atcgtacgcc gctcgatttc tttattgacc aggctgatgc
     9001 cgacgaaaat agcgatgatg acgccgatca ggttgatggc cgctaaaccc atatcgacaa
     9061 tgaccttgtg gtgctcggta atcgacagat ccgccagcaa cacggacagc ccgataagga
     9121 ggcccgcgaa cagcaccaga ttgtagagaa tcttgtctcg caaactttca cgaaacgcat
     9181 ttaaggcgat gacgccgatc gcacccatta agaggccctc ccactggtca catgcggcaa
     9241 tgacggggag gtccgttggg tggcctcctg aaaaaacaaa tcctccagcg atgctttatg
     9301 aggcgttacc gacaacaatc gcccaccctg ccgccgaatt tccccgacga gcgcatcgac
     9361 tgcgtcaggg ctgggcaaga cgatcagaca ctgttgcccc tgctggagca cacgggtggc
     9421 caacgagtga atgaaggcat tctcctcgac cttgagctgc tggcagacca cttcaacgga
     9481 ctgcgtatga tcctgccgga ccagctcatc gactcggccg ctggcaacga gacgaccttt
     9541 catgacaatt ccaacccggt cacaaatcat ctccacgtca tgcagaatgt gtgtactgaa
     9601 aaagacggtc ttcccgcgat cgcgcaggtt caagatca
//
LOCUS       DAORQN010000051         9523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_383_length_9523_cov_7.371145, whole genome shotgun sequence.
ACCESSION   DAORQN010000051 DAORQN010000000
VERSION     DAORQN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_383_length_9523_cov_7.371145"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK614_04805"
     CDS             complement(<1..721)
                     /locus_tag="PK614_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81562.1"
                     /translation="MTKHVSADDSQLVENLLVQLRQAKGLSRVELAAQAGITRQAVHA
                     IETNQYLPTTAVALRLAQALGCRVEDLFRLLPQEEVVEGEWFTASAPMQIPRNATRVK
                     VARVGARFIVRPVAELGEVMNYAVPADGLVLPLPASRHSPRSQHPSRTVQVRLLRNRR
                     VIEQEIAVAGCDPSVFLAGDYLRRQKEDCTVMGWTLGSAAAIDALKRGEVHMAGVHVV
                     DVQSGESNLPYLRRHLKGNEYL"
     gene            948..2588
                     /locus_tag="PK614_04810"
     CDS             948..2588
                     /locus_tag="PK614_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81563.1"
                     /translation="MRAYTFLGIALVAGSLIAGTITSVQAVEEGQLVKKDGKWEYYSG
                     EDPGLKYLFLKGIITQEEYDKGLKVLEKRDQIAKPNYTIDVNNGLNFRVGDKFLLKLR
                     LLTQVRYSHSAYNEGWGTVGDSRNPEILGGQVEYRAIRKQSDSNQFSVPRARLQFLGY
                     AFDPDFRYNISWAFDQTTWNQEGGSGRAGLLDAYISSWHIPWLTIQAGQQRVWFNRAT
                     ISSMATSTFADNLIVQNAFAANQQNSRDIGVSISSDEDQYKFNYAFGIWGGVGANLAR
                     EGTSVSQNVLPQTGTPGAPGAATTRTYNYDTRFMTGEMMYTARLLYKIAGNPGYGQGD
                     ILNSRTLQSAIAFGYAYNPAQNYLSSIRSDIVERAYRQAVVKAYNGRLLAGGIWDFQT
                     YEVDFIAKYQGWSFQAEGYYRHQRLRNANSGTSPFDLNTIPSAQFLGPAVNLGQAYGW
                     YAQVGKYIIPRKLEVAVRYGFMDPSTQQVDDLVKEFGAAINYSFDGTYNNRLVVDYSN
                     ITLGSGGRAPDRFPFESQPGFGRDLIENRINVQYQFFF"
     gene            2625..3449
                     /gene="modA"
                     /locus_tag="PK614_04815"
     CDS             2625..3449
                     /gene="modA"
                     /locus_tag="PK614_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB81564.1"
                     /translation="MRRRNPFFGIWLFFSMLIGITGVVPSAQAQPETLTIAAANSLKD
                     ALRKVLPRFEAEHPAINVRVIYGPSQSLRKQIEEGAPVDIFLPSLFEEIDQLETKGLI
                     IQGTKRAFAGTSLVLITGAALPAPVRTIHDLETVPVRRIAIGDPKTSSVGKVAAQFLK
                     YSKLEPKLKSQYVFGEHSRAVLDLVAKGEAEVGVVYRTDAASNDKVRILDTAPVESHT
                     PVRYGVAAVWTAKNVSGAGDFIEFLLAPQTQALFQEYGFDRVTGDVGLVQRQEVKQ"
     gene            3446..4177
                     /locus_tag="PK614_04820"
     CDS             3446..4177
                     /locus_tag="PK614_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81565.1"
                     /translation="MNGIQATVQHAHPLSRRVLLALIGLALVAGCASRPATPPKTVYQ
                     SGLSQVRVEQDPESTANAHPAVLTATEVGTLLRGVRAWERRNFVHRLFVGEADRSRAF
                     RNEEITVLAPPLAKALAQAGPTERVYFHLSHATDQGDEETTTGWISIRGPVLHLTISG
                     VHDRHSPGPDISKYDRQLPNIPEASAAHDVTFEPEEFLVKVSSGVRLFSPDQREELQI
                     RYREALSSMPVQPGLERGSERSSQP"
     gene            4207..4509
                     /locus_tag="PK614_04825"
     CDS             4207..4509
                     /locus_tag="PK614_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HRB81566.1"
                     /translation="MSILVVGGDSVSAITERAYVGGHGCVEHWSGRKTRDLTRSIPKD
                     TEAVVLVLDRVNHTLARKVRTEASRRGLPLFFQKRGRQVRAAGDRPLDLMHWLDRK"
     gene            4552..5577
                     /locus_tag="PK614_04830"
     CDS             4552..5577
                     /locus_tag="PK614_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB81567.1"
                     /translation="MNIKPLTGLTALVSLFAILPAIAGAETRELLLAAYSVPKEAYEK
                     RIIPAFVRQWKQKTGETIQVRSSFAASGAQARAVVGGLDADIAALSLESDLQQLVKAG
                     LVMHDWKQGAQKGIVTTSIVALGVRKGNPKAIQGWEDLTKPGVEVLYPSPKTSGGAIW
                     DVIAIYGAGLHHGKQQGLAGPASDQHAVDLVGRVQRHVKVMDKSGRESVTTFERGVGD
                     VIVTYENELLPRIKQGRPYELVFPAETVVVENPVAVIDRNADRHHVRDLADAFVSFLN
                     GEEAQQAFVEFGFRPTNEAVAKTTASVFVHPPHVFTIEELGGWDRVFAALFGPQGAWT
                     KAVEEAR"
     gene            5799..7385
                     /locus_tag="PK614_04835"
     CDS             5799..7385
                     /locus_tag="PK614_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81568.1"
                     /translation="MRWLHDRSIGQKFFISFGVILSLLALSLTALLVYLSRINSYVDR
                     HKRITVPAIVTAATMQRSAYEMNLTLHLFLEQITKADAADTLARLSRHAEQIRSSLDL
                     YRSTHAARTHPILLGMLTQHQRMDLADQEDRAIARIDLILQELSALWQTALTGPQRAG
                     VAPSPVAKTDGLIVELMNNLDQLVAAHRDIDVEMKVEGDLLLQQARLIALSLVAVLGL
                     VITVIYVSVNRRIAKPLQRLSVTADRVAHHDLTAQFESWPGRDEVGTLAASLSSMVTS
                     LREQTAATARKTKELEAFTYSVAHDLKGPLREIEGFSSLLEKQFADGGDAETRHQIDV
                     IRRSALRLTRMIDALLKYSRLEQQDLPRQRFNVLEMITTLITDRFSGLQGPKPKIQVA
                     LPFADLYGEPVSIRQAIANLLDNAAKFSRHASPPTITIGGTRMEHERILWVQDNGIGF
                     DPSHYDKIFGLFERLHSPQEYEGTGVGLAIVKLVMDKHDGRVWVESAPGAGSKFYLAF
                     PERAESVVDRPSSSPHPASA"
     gene            7390..8748
                     /locus_tag="PK614_04840"
     CDS             7390..8748
                     /locus_tag="PK614_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB81569.1"
                     /translation="MTMEHMRALIVDDEEFVRLVVEQALREEGCDTQTAGGGQEGLDR
                     LSTASFDCVITDLRMPGLDGRAILRWVKEHQPDVDVIVLTGHGDVKDAVAAMKDGAWD
                     FLIKDTPFDGAAVKAALARLRTVRELRRENLAARHGGYRQDAIVEGTSQAWRTVKTQI
                     AQVAPSQAPVLIQGETGSGKDVVARLLHAHSRRAGGPFIAVNCGAVSRELLESELFGY
                     EKGAFTGAAQAKPGLIAAAEGGSLFLDEIGEMPGPMQVSLLRFLDRKEYRPVGSTRTL
                     RADVRIICATNRDIQELVLQGRFRDDLLYRINTVTLHVPPLRERPEDLAVLADHILHH
                     LRIPGTATRTLSPEALAHLATYRWPGNVRELRNVIERIVLMSPNSGPITSEEVLQVLP
                     RAASASSPDDRSHLPLDEIERLHIQLVLESSGGNKTKAAQTLRVDYKTLLAKLKKYEG
                     GG"
     gene            8861..9463
                     /locus_tag="PK614_04845"
     CDS             8861..9463
                     /locus_tag="PK614_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRB81570.1"
                     /translation="MTRPGSSGEQRAQERFGTSARAAAFYAHQTLPHLNPQMQQFIAR
                     MDMVFIATADGKGACDCSFRAGEPGFVQVLDEKTLVYPEYRGNGVLASLGNILENPHI
                     GLIFPDFYQSTVGLHVNGTARVLNPSETESLSQLPARIAEAAHIKGGRHPKAWVLIGV
                     EEAYIHCSKHVPLLKRLDKQIAWGSDDEQAKGGNFFKVTP"
BASE COUNT         2064 a         2950 c         2743 g         1766 t
ORIGIN      
        1 ccaaatattc gttgcccttg agatgccgac ggagataagg aaggttcgac tctcctgact
       61 gcacgtccac gacgtgcaca cccgccatgt gcacctcgcc tcgcttcagc gcatcgatcg
      121 cggcggcact tcccagtgtc catcccatca ccgtgcagtc ttctttttgc cgtcgcaagt
      181 agtcgcccgc cagaaacacc gaagggtcgc atccagccac ggcaatttcc tgttcgatga
      241 cgcggcggtt ccggaggagc cgaacctgca ctgtacgaga tgggtgctgg gaacgtggcg
      301 agtggcggga ggctggcaac ggaagaacaa gcccgtctgc tggtacagcg tagttcatca
      361 cctcccctag ctcggcaact ggtcggacga tgaatcgcgc ccccactcga gccaccttca
      421 cgcgcgtggc gttgcgggga atttgcatcg gagccgaagc agtgaaccat tcgccttcga
      481 caacctcctc ttgcggaagc agtcggaaga gatcttccac tcgacagccg agggcctgcg
      541 caagccggag cgcgactgca gtggtcggaa gatattgatt ggtttcgatg gcatgcacgg
      601 cctgccgggt aataccggcc tgggccgcaa gttcgacacg ggagagacct ttggcctgcc
      661 tgagctggac cagtaaattt tcaacaagct gggaatcgtc agcagaaacg tgtttcgtca
      721 tggcggaccg gagaatgaca tgatgcatta catatgtcaa gcatgatgtc atgcgacaag
      781 aaagatcggt tgcgcaaaga tttttacgat ccaattgttg acaatcttaa acaacttgat
      841 atatttaata cctcaccgga atacagacgt ttagcaaagt gcatgtgaat taatactttg
      901 aaagatctca ccggtttcga ataccactaa ctcggaggtt agatcagatg agagcctaca
      961 cgtttcttgg gattgccttg gtggccggca gcttgatcgc tggaaccatc acctccgttc
     1021 aagcggtcga ggaaggtcag ctggttaaga aggatgggaa gtgggagtac tattcaggcg
     1081 aagacccggg cctcaaatac cttttcttaa aaggcatcat cacccaggaa gaatacgaca
     1141 agggcctcaa ggtcctcgaa aagcgcgacc agatcgcaaa gccgaattac accatcgacg
     1201 ttaataatgg attgaacttt cgcgtcggtg acaaattctt actcaagctc cgactgctca
     1261 cccaggtccg ctacagccac agtgcctaca atgaagggtg gggcaccgtc ggcgattcga
     1321 gaaatcccga gatcctcggc ggccaggtgg aatatcgcgc gatcaggaag caaagcgact
     1381 cgaaccagtt cagcgtgccg cgtgcccgac tccagttcct gggttatgcc ttcgacccgg
     1441 acttccgcta caacatctcc tgggccttcg accagacaac ctggaaccag gaaggcggga
     1501 gcgggagggc cgggctgctc gacgcctata tctcctcctg gcatatcccc tggttgacca
     1561 ttcaggccgg tcaacagcgc gtctggttca atcgcgcgac catcagctcg atggccactt
     1621 caaccttcgc cgacaacttg atcgtgcaaa atgcctttgc cgccaaccag cagaatagcc
     1681 gtgatatcgg cgtcagcatt tcgagcgacg aggaccagta caaattcaac tacgccttcg
     1741 gtatctgggg aggggtcggc gcaaatctcg ccagggaggg gacgtccgtc agtcaaaatg
     1801 tgctccccca gaccggaaca cccggagcgc cgggggcggc gacgacccga acgtataact
     1861 atgacacgcg cttcatgacc ggtgagatga tgtatacggc ccggttgctg tacaagatcg
     1921 ccggcaatcc tggatatggc cagggagaca ttctgaattc gcgcaccctt caatcggcca
     1981 tcgccttcgg ctacgcctat aacccggcgc agaactatct cagttcgatc cgttccgaca
     2041 ttgtcgagcg ggcctatcgg caagccgtcg tcaaggccta caacggccgt ctcctcgccg
     2101 gtgggatctg ggatttccaa acctatgaag tggacttcat cgccaagtat caaggctggt
     2161 catttcaggc cgaaggttac taccggcacc agagactgcg taatgccaac agcggcacca
     2221 gccccttcga cctcaacacc atcccatcag cccaattcct aggcccagcc gtcaatctcg
     2281 gccaggccta tggctggtac gcccaggtgg gcaaatacat cattccacga aagcttgagg
     2341 tggccgtacg atatggattc atggatccct cgactcagca ggtcgatgat ctggtgaagg
     2401 agttcggcgc tgcgatcaac tacagtttcg acggtaccta taacaaccgg ctcgtcgtcg
     2461 attactcgaa catcaccctg ggaagcggcg gccgcgcccc ggaccggttc ccgttcgaga
     2521 gccagccggg cttcgggcga gacctcatcg agaatcgaat caatgtccaa tatcagttct
     2581 ttttctagac aacctgaata cgtgacagtc acgggaggac gatcatgaga cgacgaaacc
     2641 cattcttcgg catctggctc ttcttcagta tgctgatcgg catcaccgga gtggtgccct
     2701 cggcgcaggc ccagccggag acattgacca ttgccgccgc caacagcttg aaagacgcgc
     2761 tcagaaaagt gctgccacga tttgaggcgg aacaccctgc catcaacgtg cgagtcattt
     2821 atggcccttc acagagtctc cgcaagcaga tcgaagaggg cgcgccggtc gacatcttcc
     2881 tgccgtcgct ttttgaggag atcgatcagc tcgaaacgaa gggactgatc attcaaggca
     2941 ccaaacgcgc cttcgccggc acctcgctgg tcctgatcac cggggccgca cttccagcac
     3001 cggttcgaac gatccatgac ctcgaaaccg taccggtccg gcgcattgcg atcggcgatc
     3061 ccaaaacctc atcggtggga aaagtcgccg cgcagttttt gaagtacagc aaactcgaac
     3121 ccaaactgaa gtcccaatac gtcttcggtg agcactcgcg cgcggtcctc gatctcgtgg
     3181 ccaaaggcga agccgaggtc ggcgtggtgt atcgcaccga tgccgcctcc aacgacaaag
     3241 tccgcatcct ggatacggcc ccggtcgaat cccacactcc ggtccgctac ggcgtcgctg
     3301 ccgtatggac ggcaaagaac gtttccggcg cgggcgactt catcgagttt ttgctcgcgc
     3361 cccagaccca ggcgctcttt caggaatatg gattcgatcg tgtcacggga gacgttggtc
     3421 tcgttcagcg gcaggaggtg aaacaatgaa cggtattcaa gccacagtac agcacgcaca
     3481 ccctttgtcg agacgggtgt tactcgctct gatcggactg gccttggtcg ccggttgcgc
     3541 aagcaggcct gcgacgccac ccaagaccgt ctatcagtcg ggactcagcc aagtacgtgt
     3601 ggaacaggac cctgagtcga cagccaacgc gcatcccgca gtactgacgg cgactgaggt
     3661 cgggactctg cttcgaggag tccgcgcatg ggaacgacga aacttcgtgc atcggttgtt
     3721 tgtcggggaa gcggaccgca gcagggcatt ccggaacgag gagattaccg tgttggcgcc
     3781 gccgctcgcg aaggctctgg cacaggccgg tcccaccgaa cgggtgtact tccacctgag
     3841 tcatgccacg gaccaggggg acgaggaaac gacaaccggc tggatctcca tacgaggacc
     3901 cgtcctgcac ctcaccatca gcggggtgca tgatcggcac agccccggtc cggatatcag
     3961 taaatacgac cggcagttgc cgaatattcc ggaggcctca gccgcgcacg atgtgacgtt
     4021 cgagccggag gaatttctgg tcaaggtgag ttcaggcgta cgtctcttct cccctgatca
     4081 acgagaagag ctgcagattc gttatcgaga agccctgtca tccatgccgg tgcaacccgg
     4141 actggaacgc ggaagcgaaa gaagttcgca gccctagtgc gatctgcacg agagaaagga
     4201 aatcccatgt cgatcctggt cgtcggcggt gattcggtca gtgccatcac agaacgggcc
     4261 tacgtcggcg gccacggatg cgtcgaacat tggagcgggc gcaagaccag ggatttaacg
     4321 aggtcgattc ccaaagatac ggaagccgta gtgctggtgc tggatcgagt gaaccatacg
     4381 ttggcgagga aggtccggac cgaagcgtca cgccgcggcc ttcccctctt tttccagaaa
     4441 cgggggcgcc aagtccgggc ggccggtgac cggccgctcg acctgatgca ctggctcgac
     4501 aggaagtagt cagcaactga ttttgtacgg atcactggag tgattatcaa catgaacatc
     4561 aagcccctca ccggcctcac ggcacttgta tcattgttcg caatcctacc ggcgatcgcc
     4621 ggcgctgaga cccgagagct cctgctcgca gcctacagcg tgccgaagga ggcctacgaa
     4681 aagcgaatca ttcccgcctt tgtccggcag tggaagcaga agacaggaga gaccattcag
     4741 gtccggagtt catttgccgc gtccggtgcg caggcacgag ccgtcgtcgg agggctcgat
     4801 gcggacatcg ccgccctgtc gttggagagt gacctgcagc agttggtgaa ggccggcctt
     4861 gtgatgcatg actggaagca aggtgcccag aaaggcatcg tgacgacatc gatcgtcgcc
     4921 ctgggtgtcc gcaaaggaaa ccccaaggcc atccaaggct gggaagacct caccaaacca
     4981 ggcgtcgaag tcctctatcc gagcccgaaa acatccggcg gggcgatatg ggacgtcatc
     5041 gcgatctacg gagccggcct tcaccatggg aaacaacagg gtctcgccgg ccccgcgtcg
     5101 gatcagcatg ccgtcgatct cgtcggacgc gtgcagcgac acgtcaaagt catggacaag
     5161 agcgggcgcg aatcggtgac gacgtttgag cgaggcgtgg gggatgtgat cgtaacctat
     5221 gaaaacgaat tacttccgcg gatcaaacaa ggccgtcctt acgaattggt ctttcccgct
     5281 gaaacagtcg tggtcgaaaa ccccgtcgcg gtgatcgatc ggaacgccga ccggcaccat
     5341 gtgcgggatc tggccgatgc cttcgtctcg ttcctcaacg gggaagaagc ccaacaggcc
     5401 tttgtggaat tcggcttccg gccgacgaac gaggccgtcg ccaagaccac tgcatctgtc
     5461 ttcgtccatc caccgcatgt atttacgatc gaagagctcg gcggatggga ccgggtcttc
     5521 gccgcactgt tcggtcccca aggagcctgg acgaaagcgg tggaggaagc acgatagccg
     5581 cggattgtcc agttattcgg aacgcgtggc gcgcacatgc gcctgtatcg gagcgggcca
     5641 cgcctcttcc cgcccgtctc ggaggcaagt cattcgaaca gcggttcatt gacgagcata
     5701 agcggaaggc gatgccgtcc gtgaccgccc attccacatg aaccttactg gcaccacacc
     5761 agtcactgtg ataccgtagg ccctcacacg aggcctgcat gcgctggctc cacgaccgct
     5821 cgatcggaca aaaattcttc atctccttcg gagtcatcct cagcctgctg gccttgagcc
     5881 tcacagcgct gctcgtgtac ctcagtcgca tcaacagcta tgtggaccgc cacaaacgca
     5941 ttacggtccc cgccatcgtg accgcggcca ccatgcaacg ttccgcctat gaaatgaacc
     6001 tcacgctgca tctgtttctg gagcagatca cgaaggccga tgcggccgac acccttgccc
     6061 ggctcagccg ccacgccgaa cagatccgca gttccctcga cctgtatcgt tccacccatg
     6121 cggcccgcac ccacccgatt ctcctgggca tgctgaccca gcatcaacgc atggatctcg
     6181 ccgaccagga agatcgcgcc atcgcccgga tcgatctgat cctccaggag ctgtccgcct
     6241 tgtggcagac cgcgctcacc ggacctcagc gcgccggcgt ggcaccctca ccggtcgcga
     6301 agaccgacgg cctcatcgtg gaactaatga acaatctcga ccaactggtc gccgcccatc
     6361 gggatatcga tgtggaaatg aaagtagaag gcgatctgct gctccaacag gcgcggctga
     6421 ttgcgttaag cctggtcgcc gtgctcggtc tggtgatcac cgtgatctat gtctccgtca
     6481 atcggcggat cgccaagccg ctccaacgcc tctcggtcac ggccgaccgc gtcgctcacc
     6541 acgacctgac cgcacaattc gaatcctggc ccggccgtga cgaagtcggc accttggccg
     6601 cctccctctc atccatggtc accagcctgc gcgaacagac ggccgcgact gccaggaaaa
     6661 ccaaagagct tgaagccttt acctattccg tcgcgcacga cctcaaaggc ccgttgcggg
     6721 aaatcgaggg cttctcttcc ctgctggaaa aacaatttgc cgacggcggc gacgcggaga
     6781 cccggcacca gatcgatgtg atccggcgct ccgcgctacg cctcacgcgc atgatcgacg
     6841 cgctgctcaa atattcccgg ctggagcaac aggacctgcc tcgtcagcgc ttcaatgtgt
     6901 tggaaatgat caccaccctc atcaccgacc ggttcagcgg actccagggt ccgaagccga
     6961 aaatccaggt cgccttgccg tttgccgatc tctacggtga accggtgagc atccgtcaag
     7021 cgatcgcgaa cctcctcgac aatgcagcga agttctcccg ccacgcctca ccgccgacca
     7081 tcaccatcgg cggcacccgg atggaacacg aacgcatcct gtgggttcag gataacggga
     7141 tcggcttcga cccgtctcac tacgacaaga tcttcgggct cttcgagcgg ctccacagcc
     7201 cacaggaata cgagggcacg ggcgtgggcc tcgcgatcgt gaaactcgtc atggacaaac
     7261 atgacggccg ggtctgggtg gaatccgctc ccggagcggg cagtaaattc tatctggcct
     7321 ttccggagcg cgccgaatcc gtcgtcgacc ggccatcctc ttcacctcat cctgcttcag
     7381 cgtgacctca tgaccatgga acacatgcgc gccctcattg tcgacgacga agaatttgtc
     7441 cgtctcgtcg tggaacaggc gctccgcgag gaaggctgcg acacgcagac ggcgggcggc
     7501 gggcaggaag gcctcgatcg actcagtacg gcctccttcg actgcgtcat caccgatctc
     7561 cgcatgcccg gtctcgacgg ccgggcgatc ttgcgctggg tgaaggagca tcaaccggac
     7621 gtcgatgtga tcgtcctgac cggccatggc gacgtcaaag acgccgtggc tgcgatgaaa
     7681 gacggcgcgt gggatttcct catcaaggac accccgttcg acggcgcggc cgtgaaggcc
     7741 gctctcgcaa gacttcgcac ggtgcgggaa cttcgccgcg agaatctggc ggcgcggcac
     7801 ggcgggtatc gccaggacgc gatcgtcgag gggaccagcc aggcctggcg gacggtgaag
     7861 acacagatcg cgcaagtggc gccgtcgcag gccccggtgc tgattcaggg ggagaccggt
     7921 tccgggaaag acgtggtcgc gcggctgctg cacgcgcaca gccgtcgggc cggcgggccc
     7981 ttcatcgccg tcaattgcgg agcggtgagt cgagagttgc tggagagcga gttattcggt
     8041 tacgagaaag gggcgtttac cggcgccgcc caggcgaaac ccggactgat cgccgccgcc
     8101 gaaggcggct cgttgtttct ggacgagatc ggcgaaatgc cgggaccgat gcaggtcagc
     8161 ctgctgcgct ttctcgaccg gaaggaatac cgccctgtcg gcagtacgcg cacactccgg
     8221 gccgatgtcc ggatcatctg cgccacgaat cgggacatcc aggaactcgt gctgcaaggc
     8281 cggtttcgag acgatctcct gtatcgtatc aacacggtga cgctgcatgt gccgccgttg
     8341 cgcgaacgac cggaggatct tgccgtgctg gccgatcata ttctgcacca ccttcgcatc
     8401 ccgggcaccg caacgagaac gctcagcccg gaagccctcg cacacctggc gacctaccgc
     8461 tggcctggaa acgtgcgcga acttcgcaat gtgatcgaac gcattgtcct gatgagtccg
     8521 aactccggac cgatcaccag cgaagaagtc ctgcaggtgc tccctcgggc ggcatcggca
     8581 tcatcacccg acgaccggtc gcacctgccc ctcgacgaga tcgaacgcct gcatattcaa
     8641 ctggtgctgg aatccagcgg cggcaacaaa accaaagccg cccaaaccct ccgggtcgac
     8701 tacaaaacgc tgttggcaaa actgaagaag tacgaaggcg ggggctaagc gggtctttgt
     8761 gcacttctcc acgacaacac tcatcgacag cgccagcgtg catgagggac aatcacctct
     8821 gcagcaccgt cttagacatc ttccacaagg agcgttcgat atgaccaggc cgggatcatc
     8881 aggcgaacag cgcgcacaag agcgcttcgg cacctcggct cgcgcggcag cattttatgc
     8941 ccaccaaacc ctgccccacc tgaatccgca gatgcaacag ttcatcgcac ggatggacat
     9001 ggtcttcatt gccaccgccg acgggaaggg tgcgtgtgat tgttcctttc gagccggcga
     9061 accgggattc gtgcaggtgc tcgacgagaa gacgctggtc tacccggaat accggggtaa
     9121 cggggtgcta gcgagtctgg ggaacattct ggagaatccg cacatcgggt tgatttttcc
     9181 cgacttctat cagagcacgg tcggactcca cgtcaatgga accgcgcgtg tgctcaaccc
     9241 gagcgaaacg gagagcctct cccagcttcc ggcgcgcatc gccgaagccg ctcacatcaa
     9301 gggcgggcgc caccccaagg cgtgggtcct catcggcgtg gaagaagcct acatccactg
     9361 ttccaagcat gtgccgctcc tcaagcgact ggataagcag attgcctggg gcagcgacga
     9421 cgagcaggcc aaaggcggga atttcttcaa ggtcactccc tgagccactc accagcgcgc
     9481 cggaggcgag caggccgctc gctacttttt ctccttggcg cgg
//
LOCUS       DAORQN010000052         9092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_398_length_9092_cov_41.919774, whole genome shotgun sequence.
ACCESSION   DAORQN010000052 DAORQN010000000
VERSION     DAORQN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_398_length_9092_cov_41.919774"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1102)
                     /locus_tag="PK614_04850"
     CDS             complement(83..1102)
                     /locus_tag="PK614_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRB81571.1"
                     /translation="MSQQAAETSDAYTVVVFRGSSSKPLRFSFPRKFVRKLLILAAIL
                     VVADLLVISHYVIRTGEVWQLSAFRAEAMGAREQTAAFSAAIDDLKKRLSTMGEVNQR
                     LRVMLGIDASKPAGDLANGRGGEDGPLPDGKTSVQGNGSATSGLEPRQQVSEARDANQ
                     SEVDFATESIEEVTQQVRESLEALVREAKQQEEALESLTQVAEQRSTQWASTPSIWPV
                     RGWVTSAFGPRVSPFTEKPAWHDGLDIGAQANSPVQAPALGRVVTVAFDSKMGNMVKL
                     DHGYGIETVYGHLAKSLVKEGQRVKRGDVVALVGSTGLSTGPHLHYMVKKNGQALDPT
                     KFILD"
     gene            complement(1220..1549)
                     /gene="cutA"
                     /locus_tag="PK614_04855"
     CDS             complement(1220..1549)
                     /gene="cutA"
                     /locus_tag="PK614_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRB81572.1"
                     /translation="MGANGSEVVVFVTAATAEEAERLGRIIVESRLAACANVLNGIRS
                     IFRWENKINVENECLMLIKTTLERYPELEAVIRRHHSYTIPEIIALPVIAGSAPYLEW
                     VRGETRK"
     gene            1896..2216
                     /locus_tag="PK614_04860"
     CDS             1896..2216
                     /locus_tag="PK614_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB81573.1"
                     /translation="MSFTITPKDLKSRLDKGDKLVLVDVREPWEYAIAKLEGSILVPL
                     ATLQQSLGKLDRDAEIVAVCHHGMRSADATGFLLQQGFGKVKNLIGGIDAWSTQVDPS
                     VPRY"
     gene            complement(2226..3149)
                     /locus_tag="PK614_04865"
     CDS             complement(2226..3149)
                     /locus_tag="PK614_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB81574.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLLQEGHDVVVVDNLVTGKRKNVPKA
                     AQFYKLDIENPKLERIFRNERPSIVFHLAAQMNVRRSVEDPMFDAQVNVLGTLNVLEQ
                     ASKHGARKVIFSSSGGAIYGEQLAFPAPETHITQPLSPYGISKLCGEHYLSYYHRLSG
                     IQVVSLRYANVYGPRQDPEGEAGVVAIFIQKMLRGEQAVVNGNGRQTRDFVFVEDVVE
                     SNLMAMGPEVEGVYNVGTGIETSVNDLFKIVVDLTKVEFKEVHGPAKRGEQARSVIDS
                     TKLHRDLGWEPKVDLREGLRRTVEYFRDGLG"
     gene            complement(3321..4076)
                     /locus_tag="PK614_04870"
     CDS             complement(3321..4076)
                     /locus_tag="PK614_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRB81575.1"
                     /translation="MSQKLRPERERLDRVLVSRGLVASREDAARLILAGLVRVDGVVV
                     DKAAKPTLSDAKVELTGPGSPYVGRGGEKLAGALDQFQVDPKGMTCFDVGCSTGGFTD
                     CLLQRGAARVYAVDVGYGQFEWRLRQDPRVVLMERTNIRYLEPSAIRDPIDLIVIDVS
                     FISLTLVLPCVVPYLTASGFVITLIKPQFEVGKGLVGRGGIVRDDGLREGAADKVVAC
                     AQQLGLELADRMESPIEGRKGNREILAWFRRRA"
     gene            complement(4144..4392)
                     /gene="xseB"
                     /locus_tag="PK614_04875"
     CDS             complement(4144..4392)
                     /gene="xseB"
                     /locus_tag="PK614_04875"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRB81576.1"
                     /translation="MAAVKFEYAMARLETIVAELEKGDLPLDDSLKIFEEGIRLSKTC
                     LKMLEDAERKVEILVQEKDGKKRIQAFSPGEDEPERPQ"
     gene            complement(4450..5808)
                     /gene="xseA"
                     /locus_tag="PK614_04880"
     CDS             complement(4450..5808)
                     /gene="xseA"
                     /locus_tag="PK614_04880"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRB81577.1"
                     /translation="MTGQTLPLPLLLSVSDVTRLIRDSLEAQFRDIWIEGEITNLRAP
                     SSGHLYFTLKDEQSQLRGVLFRSGASRLRFTLQEGLAIVARGRISVYEPRGEYQLIVD
                     SLEPKGVGAFQLAFEQLKERLAREGLFDEARKRPLPPFPRTVGVVTSRTGAAVRDIVA
                     VLRRRCPVANILIASVPVQGEGAAEQIAEAITTLSGMPQVEVMIVGRGGGASEDLWAF
                     NEEVVVRAIVQSRVPVVSAVGHEIDVTLADFAADYRAPTPSAAAEAVVPVLDEIVERL
                     GETSDRLYRVVRTLLEMQRHRFERSVGVMRDMRFRVQAHAQHLDELRDGLTRTLTERL
                     TLLHRGMVARQHALLAQGPHNRIQTALVVIPQLYKRLEQEARRGLLSRRQAVASYMMA
                     LDALSPLAILSRGYSVIQAIPSGRIVRLASDVAVGDVVQARLAEGRLLCLVSEVLPPS
                     MP"
     gene            complement(5846..6625)
                     /locus_tag="PK614_04885"
     CDS             complement(5846..6625)
                     /locus_tag="PK614_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRB81578.1"
                     /translation="MKVLMIGDIMGEPGRRSVARLLPKLIANHSIDVVVGNGENVAGG
                     FGITPDLVDDLFDLGVSVITTGNHAWDKKEILDVFPREPRLLRPANYPAGVPGRGSYV
                     FTTPGGESLGVLHLMGRAFMPTIDCPFQVAKREIERLKTQVSAIVVDMHAEATSEKMA
                     MGHYLDGLVTVVAGTHTHVQTADEQILPKGTAYITDIGMTGPLHSVIGIKKELAIEKF
                     LTGMPRRFEVASGPVVFCAVLMELDATLGKALSIERIRVVD"
     gene            complement(6636..8213)
                     /gene="rny"
                     /locus_tag="PK614_04890"
     CDS             complement(6636..8213)
                     /gene="rny"
                     /locus_tag="PK614_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRB81579.1"
                     /translation="MVPISLSVVAYIFVGLVGAVLGAGLYEVFRRRSALARRAEAEDQ
                     SAQIVQSAQREAENLVKEAKLEAKDLVFQSRIELEKEQKAKLAEVSNSERRVSQREEG
                     LDRKLGLLEKRDQEALKREQDLLKREETLVQKDAACAQALKQHREALERVAGLTAEEA
                     KRQLIQDLDSQARLEAAGLAKRLLEEAKENADREAREIIASSIQRVTRDYVNEATISV
                     VPIANDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIEKLMIEEAEKVIFEVGLSDFHPELVKVLGRLKYR
                     TSYGQNNLYHAREAAYICGIMASELKLDVKLAKRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGENAKVVNAIAAHHEQVEPICPETVLVAAAEALSAARPGARREALESYVKRL
                     EKLESLATVHKGVQKAYAIQAGREIRVIVKQEDLTDPECFQLSRDLAKKIEQELTYPG
                     QIKVTVIRESRFVDFAK"
     gene            complement(8773..>9092)
                     /locus_tag="PK614_04895"
     CDS             complement(8773..>9092)
                     /locus_tag="PK614_04895"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRB81580.1"
                     /translation="MNPRRWLLTKTIDVEIYGQRYSINGEADESYVKQLADMVDKQMK
                     QVAAGMRSATPAKLAVLAAFNLAHELMESERKSRQGEADADRRVASLMESIDQQMPSI
                     LSR"
BASE COUNT         1985 a         2966 c         2271 g         1870 t
ORIGIN      
        1 cgcgggcagt cgaggattac aacggagaaa gaggaaggga acaggcaaac ttctttcgaa
       61 atctggaagg aatacgaaaa gactagtcga gaataaactt ggtcggatcc aacgcttgac
      121 cgttcttctt gaccatgtaa tggagatgcg gtcccgtgga aagcccggtg ctgccaacaa
      181 gcgcgaccac atcaccgcgc ttcactcgct gcccttcttt caccaacgac ttggcaaggt
      241 gtccatagac cgtctcaatc ccatatccat ggtccagctt aaccatattg cccattttcg
      301 agtcaaaggc cacggtgacg acacgcccga gcgccggagc ctggacgggg gaatttgcct
      361 gagcaccgat atccagaccg tcatgccagg ccggcttttc ggtgaacggc gaaacccggg
      421 ggccaaacgc cgaggtcacc catccgcgta caggccagat ggagggagtt gacgcccact
      481 gagtcgaacg ctgctctgca acttgggtca ggctttcgag ggcttcctcc tgttgcttag
      541 cctcccggac gagggcctca aggctttcgc gaacctgctg agtgacctct tcaatacttt
      601 ccgtagcaaa atcaacctcg gactgattgg catcacgcgc ttcggagacc tgctgccgcg
      661 gttccagccc ggaagtggct gatccattcc cctgcacact ggtttttcca tccggcagcg
      721 gaccgtcctc accaccccgt ccattcgcca aatccccggc cggcttgctg gcgtcaattc
      781 ccagcatgac tcgaagccgt tgattgacct cgcccatggt cgagagacgc tttttcagat
      841 catcaatcgc agcggagaag gcagcggttt gctcacgcgc acccatggct tcagcccgaa
      901 acgctgataa ctgccagact tctccggtcc gaatcacata atgagagata acgagcagat
      961 ctgccacgac caggatcgca gccaggatca ggagcttgcg cacgaatttc cgtggaaaac
     1021 taaaccgcaa cggcttggag gaggaccctc gaaagaccac cacagtatag gcatcacttg
     1081 tttcggccgc ttgttggctc ataggacgct ctccccttca cggattcaac caagaattca
     1141 gccactgact tttgcagtgt actgatctct ggcgcgatgg tcaacccttt gttttcaaaa
     1201 attacattat tagaatcaac tacttacgag tctctccccg aacccattcc aggtagggcg
     1261 ccgatcccgc aatgaccggg agtgcgatga tctcagggat agtatagcta tggtgccgtc
     1321 gaatgactgc ttcgagttcc gggtatcgct ccagggttgt tttaatgagc atcaggcatt
     1381 cattttcgac gttgatctta ttctcccaac ggaaaatcga tcgaatgcca tttaagacat
     1441 tcgcgcaggc agcaagtctg gactccacaa tgatccgccc gagtctttct gcctcctcgg
     1501 ccgttgccgc agtcacaaac accactacct ctgaaccgtt cgctcccact tctcccccac
     1561 agctctaagc aaattgcgtg ccgacacaga acagcgaata ctgtttgcgc ttcagttatc
     1621 aagcacttag ggcagaattc taccccatct tcataccgat tacgctcaaa aaactaggca
     1681 ggatttgcac aattcgacaa aaaacgacca ttctccagag gcgttacctg ccgaccgact
     1741 gtcaggccac ggccctcgtc ctctcatcca tacgcttcgg tctgaatccg caaagtcttg
     1801 agatcagacc ccacgcgacg agtggctgac cacaccggag ttgacacccc tttttcggct
     1861 ccttatgatc cgaccgtata gatggaggtg cactgatgag tttcacgatt acccctaagg
     1921 atctcaagtc gaggctggat aaaggcgaca agctggtgct ggtagacgtc cgggagccat
     1981 gggaatatgc catcgccaag ttggagggat ccatcctggt cccgctggcg accctgcagc
     2041 aatctcttgg caaacttgac cgtgacgccg agattgtcgc cgtgtgccac cacgggatga
     2101 gaagtgcgga tgcgacaggg tttctgctgc agcaaggctt cggaaaggtg aaaaacctga
     2161 tcggaggcat tgatgcctgg tctactcagg tagacccatc cgtgccccgc tattagctca
     2221 agcggctatc cgagcccgtc gcggaaatat tcgaccgtcc gccgcagtcc ctcgcgcaga
     2281 tcgaccttcg gttcccaccc aaggtcacgg tggagctttg tggagtcgat gacgcttctg
     2341 gcctgctcgc ctcgcttcgc cggaccgtgc acttccttaa actctacctt ggttagatcg
     2401 acgacgattt tgaagaggtc gttcacggac gtctcgattc ccgtgcccac gttgtacacg
     2461 ccctccacct cgggacccat cgccatcaaa ttcgattcca ccacgtcctc gacaaacaca
     2521 aaatcgcgag tctgacgccc gtttccgttg accaccgcct gttcgccacg cagcatcttt
     2581 tgaatgaaaa tggcgacgac tcccgcctcc ccttccggat cctgtcgcgg gccgtacaca
     2641 ttggcatatc gcagactgac cacctgaata ccgctcaacc ggtggtagta ggataggtaa
     2701 tgctccccgc ataacttgct gatgccgtag ggcgacagtg gctgggtgat gtgagtttcc
     2761 ggggcaggaa acgccagctg ctcgccataa atcgccccgc cggatgagga gaagatgacc
     2821 ttgcgcgcgc catgtttcga cgcttgctcc agcacattga gcgtccccag cacattgacc
     2881 tgcgcatcga acatcggatc ttcgaccgaa cgccgcacgt tcatctgagc ggcgaggtgg
     2941 aacacgatcg acggccgttc gttgcgaaaa atccgttcga gcttgggatt ttctatatcc
     3001 agtttgtaga attgggccgc cttggggacg tttttccgct ttcctgtgac aagattatcg
     3061 accaccacaa catcatgccc ctcctggagc agacgatcca cgacatgtga cccgataaac
     3121 cctgcgcccc ccgtgaccaa gactttcata cgtctcctcg cctctccttc atctgcggat
     3181 gtgccgatcc cctgaccgaa caattgaggg tcagcctaca ccttctgcag gcttccgcaa
     3241 agtgttttcg tgttgccctt gcgcgtgaac gtcccggacg aaacccgccg cagacccact
     3301 ggtatccata ctcgtacccc tcacgccctg cgccggaacc aagccaggat ttcccgattg
     3361 ccctttcggc cctcgatggg cgattccatt cgatccgcca actccagccc caactgctgc
     3421 gcgcacgcca ccactttgtc ggccgcccct tcccgcaagc catcatcacg aacaatccca
     3481 ccccgtccga caaggccttt ccccacctcg aactgcggtt tgatcaacgt gatcacgaac
     3541 ccagacgcag taaggtaggg cacaacacag ggcagcacga gggtcagcga gataaacgac
     3601 acatcgatga caatcagatc gatcggatcc cggatcgcac tcggctcaag ataccggatg
     3661 ttggtccgct ccatgagcac cacgcgcgga tcctggcgta gccgccactc aaactgacca
     3721 taccccacat cgaccgcata gacacgcgcg gcaccgcgct gcagcaggca atcggtaaat
     3781 cccccggtcg aacacccgac gtcgaagcac gtcattcctt tcggatcgac ctgaaattga
     3841 tccagcgcgc cggctaactt ctcgcccccg cgaccgacat agggcgaccc cggcccggtc
     3901 agctccacct tcgcatccga caaggtcggt ttcgccgcct tatcaaccac caccccatcc
     3961 acacggacga ggccggccag gatgagtcga gccgcgtctt cccgactggc aaccaaccct
     4021 cggctgacca gcacgcgatc cagccgttcc cgctctgggc gtaatttctg gctcataggc
     4081 tgtgatggaa gtttaattgc gacagacacg ggcaacgtga aagcccgtgc atcgatcaga
     4141 ccgttactga gggcgctccg gctcatcctc accgggagaa aacgcttgaa tccgcttctt
     4201 gccgtccttc tcttgcacca ggatttcaac cttccgctcg gcatcctcca gcatcttcag
     4261 gcaggtcttg gacaaccgaa tcccctcctc gaaaatcttc agcgagtcat cgagaggaag
     4321 atcgcccttt tcaagttcgg caacaatcgt ttccaatcgt gccattgcat attcaaactt
     4381 cacggcagcc acaacaactc ccttgctcat tcatcaaaca gaatattatt gcctgcccca
     4441 ccggtgaggt caaggcatcg aaggcggcag cacctcactg acgagacaca acagacgccc
     4501 ctcggccagc cgcgcctgca ccacatcccc caccgccacg tctgacgcca gccggacgat
     4561 ccgcccggac ggaattgcct ggatgacgct atacccgcgg cttaagatcg ccaatggact
     4621 gagagcgtcc aaagccatca tgtacgacgc aaccgcttgc cgcctggaca gaagtccccg
     4681 tctggcttct tgttccaacc gtttatacag ttgcgggatc accaccagcg cggtctgaat
     4741 ccggttgtgc ggtccttgag ccagcaaggc atgttgccgc gccaccatcc cccggtggag
     4801 cagagttaat cgttcggtca gcgtgcgcgt caatccgtct cgcaattcat cgagatgctg
     4861 ggcatgcgcc tgcactcgaa agcgcatgtc gcgcatcact ccaaccgatc gctcgaaccg
     4921 atgacgctgc atctccaaca aagtgcgcac gactcgatac agacgatcag acgtctcgcc
     4981 tagtcgctcc acgatctcgt ccaataccgg caccaccgcc tcggccgcag ccgagggtgt
     5041 cggagcccga tagtccgcgg caaagtcggc aagggtcaca tcgatttcat gccccaccgc
     5101 agacacgacc gggaccctcg actgcacaat ggcgcgaacg accacctctt cgttgaacgc
     5161 ccacaaatcc tctgaggcgc cgcctccacg cccgacgatc atcacctcaa cctggggcat
     5221 tccgcttaac gtcgtgatcg cctccgcaat ctgctcggcg gctccctcgc cctggaccgg
     5281 cacggaagcg atgagaatat tcgcgaccgg acagcggcga cggaggaccg ccacaatatc
     5341 gcggaccgcg gccccggtgc gagaggtcac cacgccgacc gtgcgcggga agggaggcag
     5401 gggacgcttt cgcgcctcgt cgaacaatcc ttctcgcgcc aaccgctcct tgagttgctc
     5461 gaacgccagt tgaaacgccc cgacgccctt cggttccagc gagtcgacga tcagttgata
     5521 ctcgccacgc ggctcataca ccgagatgcg cccgcgcgcg acgatcgcca ggccctcctg
     5581 cagggtgaac cgaagccgcg aggccccgga tcgaaagagc accccgcgaa gctggctctg
     5641 ctcatctttc aatgtgaaat acaggtgtcc ggaagacggt gcgcgaaggt tagtaatttc
     5701 gccctcgatc cagatatccc ggaattgcgc ttccagggaa tcacggataa gtcgcgtcac
     5761 gtcggatacg gaaagaagca gaggaagcgg aagggtttgg ccggtcatag cttaccgagc
     5821 ctgagaaaat ggctaggcga acagatcaat ccaccactcg gattcgttcg atcgacaaag
     5881 ccttgccgag cgtggcatcc agttccatca ataccgcgca gaagacgacg ggacccgagg
     5941 ccacttcaaa gcgccgcggc attccggtga gaaacttttc gatcgccagt tcttttttaa
     6001 ttccgatcac cgaatgaagc gggccggtca tgccgatatc cgtgatgtac gccgtgcctt
     6061 tcggaaggat ttgctcatca gccgtctgca catgggtgtg cgtccccgcc acaaccgtca
     6121 ccagcccatc caggtaatgc cccatcgcca tcttttcaga cgtcgcctcg gcatgcatat
     6181 cgaccacgat cgcggagacc tgcgtcttga gacgttcgat ttcccgcttc gcgacctgaa
     6241 acggacagtc gatcgtcggc atgaacgccc gccccatcaa atgcaggaca cccaacgatt
     6301 cgccgccggg cgtggtaaac acataactgc ctcggcccgg cactccggcg ggataattcg
     6361 caggacgcaa gagacgcggt tcacgaggaa acacatcgag gatttccttc ttgtcccacg
     6421 catgattccc cgtggtgatg accgacaccc cgagatcgaa aagatcgtcc accaggtccg
     6481 gagtaatacc gaatccgccg gccacgttct cgccgttgcc gaccacaaca tcgatggaat
     6541 ggttggcaat cagtttcggc aggaggcgag ccacggaccg acgccctggc tcgcccatga
     6601 tatcgccgat catcagaacc ttcataaccg ttcgctcatt tcgcaaaatc gacgaaccgg
     6661 ctctcccgga tcaccgtcac cttgatttgc cccggataag tcagctcctg ctcaatcttc
     6721 tttgccagat cccgggacaa ctggaaacat tccgggtctg tgagatcttc ctgcttcacg
     6781 atcacgcgaa tttcacgacc cgcttgaatc gcataggcct tctgcacacc cttgtgaacg
     6841 gtcgccagcg actccaactt ctccaaccgc ttgacatacg actccaacgc ttcacggcgt
     6901 gcccccggcc gcgccgccga cagcgcttcc gccgccgcca ccagtacggt ctccggacaa
     6961 atgggctcaa cctgttcgtg atgcgccgca atggcattca ccactttggc gttttcgccg
     7021 tacttcttgg cgatctccgc acccagcatg gcatgcggtc cttcttcctc gtgactgacg
     7081 gcctttccga tatcgtgtaa caaggcaccg cgcttggcga gcttcacgtc gagtttcaac
     7141 tcggaggcca tgatgccgca gatgtaggcc gcttcccgcg catggtacag attattctgc
     7201 ccatagctgg ttcggtactt gagccgtccc aacaccttga ccaactcggg atgaaaatca
     7261 gacaagccaa cctcgaagat caccttttca gcctcttcga tcatcagctt ctcgatatcg
     7321 accttcacct tttcgacgat ctcttcaatg cgcgtcggat ggattcggcc gtcgtgcatg
     7381 agccgctcaa gcgagacctt ggcaatctca cgacgaagcg gatcgaaccc tgaaatgatg
     7441 accgcttccg gcgtctcatc gatgatgagg tcgatccccg tcgccgcctc gatcgcccgg
     7501 atgttccgcc cttctcgacc gataatccgg cccttcatgg catcgttggc aatcggcacc
     7561 acggagatag tggcttcatt cacataatcc cgggtcactc gttgaatcga gctggcgatg
     7621 atttcccgcg cctcccggtc ggcattttcc ttggcctctt cgagcaggcg cttggccagg
     7681 ccggccgctt ccaaccgcgc ctgactatcc agatcctgaa tcagttgccg cttcgcttcc
     7741 tcagccgtca agccggccac ccgctcaagg gcctcgcgat gctgtttcag cgcctgcgcg
     7801 caggccgcgt ccttctgtac cagtgtctcc tcacgcttca ataagtcctg ctcacgcttg
     7861 agcgcttcct gatcccgctt ctcaagcaag ccgagcttgc gatcgagtcc ctcttcccgt
     7921 tgggacacac gccgttccga attggaaact tcggccagct tggccttttg ttccttttcg
     7981 agttcgatcc tggactgaaa gaccagatcc ttggcctcca gcttggcctc tttgacgaga
     8041 ttctctgctt cacgctgagc cgattgaacg atctgcgccg actgatcctc ggcctcggca
     8101 cgacgagcca acgccgaccg acggcgaaag acctcataca gcccggcacc caggaccgct
     8161 cccactaatc cgacaaaaat gtacgcaaca acgctgagag aaatgggaac cacctccctt
     8221 acggcagcgg gacagtaaac actgtcaccc tgcgaatagt cacagccagt gttaaaggga
     8281 gagcaaggag cgtgagaaag gaatgtctac gcgtgagggg gaaacatctg acttcttgag
     8341 ccagtagaaa cgaagcgtgg aatcgccgcc gtcaattcac gtggaggagt ggtcgacttc
     8401 gacccgaatc aatacgtctg tcacatcgaa ggcgcacaga ccaagccccc cggtcgggga
     8461 tcgtcacagg ttgcgagcac agcgcaggcc atagcacatc cgtcagcacc tgctcttcgc
     8521 cccttgctac acgttgcgaa cacacctgtc gaacagacat gccagcctgg gagccttctc
     8581 tcatccacac catcacgacc actgtgaaca acagcacaaa aaccagcgat tccatatgtt
     8641 tagcgctcaa aaaccgtcgt cagattttcc gtccttgttc gaagcttcct ttcctctcct
     8701 gctcttgttg accctcaggt caatacaaca cagaaacgat aacaaagcag tacagtgaaa
     8761 gcaaggcgaa actcaccgtg acaggatgga cggcatctgc tgatcgatcg attccatcag
     8821 ggaggccacc cgacgatccg cgtcggcctc tccctgccgg gactttcgct ctgattccat
     8881 caattcgtgg gcaaggttga atgcggcaag gacggccaac ttggccgggg tggccgaccg
     8941 catgccggcc gccacctgtt tcatctgctt atcgaccata tcggccaatt gcttcacgta
     9001 cgattcgtcc gcttccccgt tgatgctgta ccgctggcca tagatctcca cgtcaatggt
     9061 cttagtcaac agccacctcc ttgggttcat cc
//
LOCUS       DAORQN010000053         9080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_399_length_9080_cov_8.794349, whole genome shotgun sequence.
ACCESSION   DAORQN010000053 DAORQN010000000
VERSION     DAORQN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_9080_cov_8.794349"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1102
                     /locus_tag="PK614_04900"
     CDS             137..1102
                     /locus_tag="PK614_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRB81581.1"
                     /translation="MRTLGWVTDESYTLVECRAPIYSIREQVELPCKIPRQTTLLWSP
                     HYNIPLCYSGRLVVTIHDTFHLAMPQFVPGWHRRAYARLMFRAVRRKACEIFCVSQFG
                     KRQLMAFCPQGTQPVTVTPNGVDESWAQFPKEPRSHERPYILFVGNLKPHKNLVRLLE
                     AVELIRQRIPHDLVIVGRSEGFITGDHQVRARAAKLGDRVRFVDELDHDDLLLRRYYA
                     CADLLVLPSLYESFGLPALEAMACGCPVVLSKVGGMPEVYGDAAAYCDPLDSQDMADQ
                     ILRVLTNRELRAELIERGLRCAHRLNWEETATKISTSIKYLLAMP"
     gene            1180..2355
                     /locus_tag="PK614_04905"
     CDS             1180..2355
                     /locus_tag="PK614_04905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81582.1"
                     /translation="MKVAIIHDWLTGMRGGERCLEAFCELFPDADIYTLLHIKGSVSP
                     TIERHRIRTSFIQSLPLSRRWYRYYLPLFPLAIERFRLGSYDLLLSSSHCVAKGIRPP
                     AGGKHLCYIHSPMRYVWDQFDNYARGGRSGVVARLGMELFRKRLQAWDVASAARVDQF
                     VANSHNIAGKVQRYYNRSAVVLHPPVDWESFQAAPTSEDFYLMVTAFAPYKRVDLAIQ
                     ACNVMKRRLKIIGKGQEEARLQKLAGPTVEFLGWQPDDVVRDHYARCRALLFPGEEDF
                     GIVPLEAMACGKPVLAFGRGGALETVVPLTGAGSVEGAPVRGGTDSVTGAGNVATGVF
                     FFTQSVESVVEAMESFERRRTEFDPHAIRDHVAAFDRRLFKECMKAFAMGALTGTAS"
     gene            2700..3764
                     /locus_tag="PK614_04910"
     CDS             2700..3764
                     /locus_tag="PK614_04910"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRB81583.1"
                     /translation="MLLYFWLLNLITLGFSRVLFREALRFLRRHGYNRRHALVIGTSR
                     LGRRVVDALAQHPELGVKVHGFLSADSRKVGERINGVPVIGRYDDLQERVQSGVDIVF
                     VCLPPEDEQWAEKMFTCLATTMVEVKALPAMCEFVSLRAEAEMFEGLPLITLQGSPLY
                     GWNLVIKRVLDVVGASAALVVFSPVLCAIAALVKLTSPGPVFFRQLRMGLDGQAFEML
                     KFRSMKLDAESETGPVWTQPNDDRRTPIGAFLRRTSLDELPQFWNVLRGEMSIVGPRP
                     ERPEFIARFRETLPQYMLRHKMKAGITGWAQINGWRGNTSLERRIEHDLYYIEHWSIW
                     FDCKIMLLTAWRGFIHRHAY"
     gene            3901..4440
                     /locus_tag="PK614_04915"
     CDS             3901..4440
                     /locus_tag="PK614_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRB81584.1"
                     /translation="MPSTTRRTKTSVKPRFSLQGTLRGIRLFATDVDGVLTDAGMYYS
                     ESGDEWKKFNTHDGMGIKLLQKAGIVTAIITQESTKIVMRRAQKLTIPEVHQGAYDKL
                     AVLKELIARHGFSMDQVAYIGDDVNDLQALGAVGFSASPADGMPPVLKTVRYVCKQKG
                     GEGAVREVADLILAARQPR"
     gene            4482..>4784
                     /locus_tag="PK614_04920"
     CDS             4482..>4784
                     /locus_tag="PK614_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB81585.1"
                     /translation="MDPMKHLYPVILAGGSGTRFWPLSRHLYPKQLLRIIGDETLIQQ
                     TMRRVLSCAKPEQVIISTNPGQADSIRVQLGEWKSALKDNYVVEPVGRNTAPAIALV"
     assembly_gap    4785..4794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4795..5938
                     /locus_tag="PK614_04925"
     CDS             <4795..5938
                     /locus_tag="PK614_04925"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRB81586.1"
                     /translation="ELLLRDPHAMMLVLPADHVVTGEKAFKAAVALGVQLAEQGHLVT
                     FGIKPTRPETGYGYIQPNRRKSLAKKGRLTGHPVARFVEKPNRIKAMQYLKNGNFYWN
                     SGMFLWKAATILEEIQQHQPQLAKAIQRVHALMASSAEPALVEAAYKKVPSVSIDNGV
                     MEQSANAAMIPVGFGWSDVGNWSSLEEVAPRDKAGNVVSGRVIDMDSTNSVLYADRRV
                     VATIGLSDMVVVDTPDATLICPKSRSQDVKQMVEILKQQGAPEHLEHLTVFRPWGSYT
                     VLEEGPGYKVKRVTVNPGGRLSLQLHHKRSEHWVVIAGTARVTRGDELLDLRVGQSTA
                     IPVETPHRLENLGSETLHIIEVQNGPYLGEDDIVRFQDAYGRTSVR"
     gene            6020..7420
                     /locus_tag="PK614_04930"
     CDS             6020..7420
                     /locus_tag="PK614_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRB81587.1"
                     /translation="MALFREYDLRGIVGEELTEAIAEQVGRAYATMAREQGASRISLG
                     RDGRLSSPGLQEALLRGLLAGGLDVVDLGLCASPLLYFSLFTLPVDGGIMITGSHNAA
                     EYNGFKICIGKEAIHGEEIQRLRRVMEAGQFATGSGRLSSHPIIPDYLEHLKTNFSGV
                     RADHLHVVIDCGNGAASLVAKQALEQMGCRVTGLYDELDGRFPNHHPDPTVVENLQDL
                     IATVREKKADVGIGYDGDADRIGAIDERGEILWGDRLMIVYARDILTQRPGTTFISEV
                     KASQCLYDDIAAKGGRPIMWKTGHSLMKAKLKAESAVLAGEMSGHMFFADRYFGFDDA
                     IYASCRLVEILAKTKQSLSSLVADLPQTTVTPEIRVDCPDTLKFQLVDQVRTQLAAYL
                     AEGKPVGASNLRMRELVTIDGVRAIFEDGWGLIRASNTQPALVLRFEAPSPARLDVIR
                     ATIETELAQARRVLSA"
     gene            7422..8234
                     /locus_tag="PK614_04935"
     CDS             7422..8234
                     /locus_tag="PK614_04935"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRB81588.1"
                     /translation="MLCSSDSFRLSLFLAVTILATVGASAPLLSYATPDGQILAPAAL
                     PFANGERLSYDVTWLGMRAGIATMIVQEGPVEGARPQLTLAMTARSSPTVTKFYPVDN
                     RGVSVVDRESFLPRHMTFARREGKRFNDFDYTFRHTEGLVTAVKDGKSDELPIPSDAQ
                     DAMSCLYYVRKVLPFVPGASLAMTVHHDKKNYKMDVRVEALETVEGVWGKRQTARVVV
                     IMPFQGIFLNEGNIRVWFTNDEHRVPVRMKAKVVVGSIVAELTDGYRPTSQP"
     gene            8368..>9080
                     /locus_tag="PK614_04940"
     CDS             8368..>9080
                     /locus_tag="PK614_04940"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRB81589.1"
                     /translation="MAKFPITLSRFIIEQQAAHPEATGEFSVLLTQIGLVGKMIAHDL
                     RRAGLNKILGTTGETNIQGEVVKKLDQIANDTFVRVFESSGLVCVLASEEMEKPLKMV
                     HEGAKYMLLVDPLDGSSNTDVNMPLGAIFSIRRSKLGQRLPVEDELLQRGTEQIAAGY
                     VLYGASTMLVFTVGQGVHGFTLEPSIGEYLLSNENIRIPSRGKVYSVNEGNYHRWPSG
                     TQKYLDSLKVQDKATGRPY"
BASE COUNT         1780 a         2446 c         2839 g         2005 t
ORIGIN      
        1 tggctaagta atgctcgcca tcgatgtccg aatgattcgt tgcgcaggga tcggaaccta
       61 tcttcgtcag ttggttccac gagttgtagc ggccttgcct ccttatccga tctccttgat
      121 tggggatact gcccacttga gaaccttagg ctgggtgacg gatgaaagtt atactctcgt
      181 ggagtgccgt gcgccaattt attccattcg tgaacaagtc gaactgccgt gcaagatacc
      241 gaggcagaca acactgttgt ggtctccgca ctacaatatt cccttgtgct attcaggccg
      301 attggtcgtc acgatccacg atacgtttca tttggccatg ccgcaatttg tgccagggtg
      361 gcataggcgt gcctatgcac ggttgatgtt tcgtgccgtt cgccgcaagg cctgcgagat
      421 tttttgcgtg tcgcaattcg gaaagcgtca attgatggca ttttgtcctc aaggaaccca
      481 gccggtcacg gtgacaccca atggtgtgga cgagtcatgg gcacagtttc ccaaggaacc
      541 gcgatcgcac gagaggccct atattctttt cgtcggcaat ctgaagccgc acaagaacct
      601 cgtgcggttg cttgaggcag tcgagttgat acggcagcgc ataccgcatg atctcgtcat
      661 tgtggggcgt agtgagggat tcattacagg agatcaccag gtgagggcga gggctgcgaa
      721 attgggtgac cgcgtacggt ttgtcgatga actcgaccat gacgatctgc tccttcgacg
      781 ctactatgcc tgcgccgatc tcttggtgct gccgtctctt tatgaaagct tcggccttcc
      841 tgcccttgag gctatggcct gtggatgtcc tgtcgtcttg tcaaaggtgg gagggatgcc
      901 cgaggtctat ggagatgcgg cagcctactg cgatcctttg gacagtcagg atatggccga
      961 tcagattctt cgagtcctga cgaatcgaga acttcgtgca gagctcatcg aacggggctt
     1021 gcgatgtgcg catcgattga attgggagga aacggcgaca aaaatttcaa cgagtatcaa
     1081 gtacttgttg gccatgccct gataaggtct ggatgcgtct ggcgaatcat cgatagacgg
     1141 taaggtgtgt gcatgcctga tgaggtgtga ggactcgata tgaaggtcgc gatcatccat
     1201 gattggctga cgggcatgcg gggcggtgag cgttgcctgg aagcgttctg cgagctgttc
     1261 ccggacgccg acatctatac cctgctgcac atcaaaggga gcgtgtctcc cacgatcgag
     1321 cgccatcgaa tcaggaccag tttcattcag tcgctccccc tgtccaggcg gtggtaccgg
     1381 tattatctcc cgttgtttcc ccttgcgatc gagcggttcc gtctcggctc ctatgatttg
     1441 ctcttaagtt cgagccattg tgtggcgaag ggcatccgcc cgccggcggg agggaaacat
     1501 ctttgctaca tccattcgcc catgcggtac gtgtgggatc agttcgacaa ctatgcccgc
     1561 ggcgggcgat cgggagtcgt ggcgagattg ggtatggaac tgttccgaaa gcgtttgcag
     1621 gcctgggatg tggcctccgc cgcgcgagtg gatcagtttg tggccaattc ccacaatatc
     1681 gccgggaagg tccaacgcta ctacaaccgc tcggcggtcg tgctgcatcc accggtcgat
     1741 tgggagtcat ttcaagccgc gccgacatcc gaagacttct atttgatggt cacggccttt
     1801 gctccgtaca aacgggttga tctggccatt caggcgtgta atgttatgaa acggcggctg
     1861 aagatcatcg gcaaggggca ggaggaggcg agattacaaa aactcgccgg cccgacggtt
     1921 gagtttctgg gttggcagcc ggacgatgtg gtgagggacc attatgcacg ctgtcgggcg
     1981 ttgctctttc caggcgagga agattttggt atcgtcccat tggaagcgat ggcctgcggc
     2041 aagcctgtgc ttgccttcgg gcgcggggga gcgctggaga ccgtggtgcc gttgacgggt
     2101 gccggatccg tcgagggcgc acctgtgcga gggggaaccg attctgtgac cggtgcgggg
     2161 aacgttgcca cgggagtgtt tttcttcact cagtcggtcg aatccgttgt cgaggcgatg
     2221 gagtctttcg agcgtcgccg aacggagttt gatccgcatg ccattcgtga ccacgtggcc
     2281 gcattcgacc gccggctgtt caaggagtgc atgaaagcct tcgcgatggg ggcgttgacc
     2341 ggtacggctt catagaggga gcatgctcaa acgccactcc caatttctca aaagtctgct
     2401 gttttgtatc gatctcgggc tgatctgcgc ctgctgggtc ggagcctact accttcgttt
     2461 ttcagagatc gtggaacctg ccaccaaggg cattcctcct ctccggatct atctgctgtt
     2521 gttggtgccg atcatcgcag tctggggcat gtcgttccaa gccttcgacc tctaccgccc
     2581 gcgacggatg ggcacgcggt tgtcggagtt tctcgacgtg gcgaaagcca ataccctctc
     2641 cgtgctgatt ctggtggcat tgaccttttt tcttccggca gtacgaatat tcccgactgg
     2701 tgctcctcta tttctggctg ttgaacctca tcaccctggg cttttcgcgg gtgctgttcc
     2761 gggaggcgct tcggttcctg cgtcggcacg gatacaatcg acgccatgcg ctggtcatcg
     2821 gcaccagccg gctgggacgc cgggtggtgg acgcgctcgc tcagcatccg gagctggggg
     2881 tgaaagtgca tgggttcttg agcgccgatt cccggaaggt gggtgagcgg atcaatgggg
     2941 tgccggtgat cgggcggtac gatgacctgc aggaacgtgt tcagtcaggt gtcgatatcg
     3001 tctttgtctg cctgcctccg gaggacgagc agtgggctga aaaaatgttt acctgtttgg
     3061 ccacgacgat ggtcgaagtg aaggccttgc ccgcgatgtg cgaatttgtg agcctgcgcg
     3121 cggaggccga gatgttcgag ggccttccgc tcatcacgtt gcagggatcg ccgttgtacg
     3181 ggtggaatct ggtcatcaaa cgggtgttgg atgtggtcgg cgcatcggcg gcgctggtgg
     3241 tattctcccc ggtgttatgc gcgatcgcgg ccttggtgaa actgacttcg cccggcccgg
     3301 tctttttccg tcaactccgt atgggattgg acgggcaggc gttcgaaatg ttgaaattcc
     3361 gttccatgaa gctcgatgcg gagtcggaaa ccggcccggt ctggacgcaa cccaatgacg
     3421 atcgccggac tccgatcggc gcattcctgc gacgaaccag cctggatgaa ctgccgcaat
     3481 tctggaacgt gctgcgcgga gagatgagta tcgtcggccc caggcccgaa cggcccgagt
     3541 tcatcgcccg gtttcgggaa acgcttccgc agtacatgtt gcggcacaag atgaaagccg
     3601 ggatcaccgg gtgggcgcag atcaacggtt ggcggggcaa cacgtcgctg gaacgacgga
     3661 tcgagcacga cctgtattac atcgagcact ggtcgatctg gttcgactgt aagatcatgc
     3721 tcctcacagc ctggcgagga tttatccacc ggcatgcgta ttgatatttt gtgccgccgt
     3781 gcccaaccgg ctgcttccgg ccggagcggc attggggcgc gccggacact ccgacatccc
     3841 gagttttctt gagtttcact tgatggcttg ttaacatcgg ccgcattcga aagcagctgc
     3901 atgcccagta caacacgccg caccaaaact tcagtcaagc ctcgtttctc tttacaaggg
     3961 actctccgag ggatccggtt attcgcgacg gatgtggatg gagtcttgac cgacgccggc
     4021 atgtactact ccgagtccgg cgacgaatgg aagaagttca atacgcatga cgggatgggg
     4081 atcaagctct tgcagaaggc cggcattgtg acggcgatca ttacgcagga gtcgacaaaa
     4141 atcgtgatgc gccgcgcgca gaagttgacg attcccgaag tgcaccaggg ggcgtatgac
     4201 aagctcgccg tgctcaagga gctgattgca cggcatggat tctcgatgga tcaggtggcc
     4261 tacatcggtg atgacgtgaa cgatctccag gccctgggtg cagtcggatt ttcggcctcc
     4321 ccggccgacg ggatgccgcc ggtgctgaag acggttcgat atgtgtgcaa gcaaaagggc
     4381 ggagagggcg cggtacggga agtggccgat ctcattctgg cggctcggca gccacgctga
     4441 gctcctcgca agcacgcgat tttgacggtg aagggcactc catggatccg atgaagcatc
     4501 tctatccggt tattctggcc ggcggcagcg ggacgcggtt ctggccgcta agccgccatc
     4561 tctatccgaa gcaattgcta cggatcatcg gcgacgagac gctcattcag cagacgatgc
     4621 gacgcgtcct ctcctgcgcg aagcccgagc aggtcatcat ctcgaccaat ccgggccagg
     4681 cggattcgat tcgggtgcaa ttgggagagt ggaaatccgc gttgaaagat aactacgtgg
     4741 tcgagccggt cggtcgcaat acggcccccg ccattgcgct ggtgnnnnnn nnnncgagct
     4801 tctgctgcgc gatcctcatg cgatgatgct ggtcttgcct gcggatcatg tcgtaaccgg
     4861 tgagaaggcg ttcaaggcgg cggtggcgct gggtgtgcaa ctggccgaac agggacatct
     4921 ggtgacgttc gggatcaagc cgaccagacc tgaaacgggg tatgggtata ttcagcccaa
     4981 ccggcggaag tctctggcca agaagggacg actgacgggc catccggtcg cgcgatttgt
     5041 cgagaagccg aaccggatca aggcgatgca gtatctgaag aacggcaact tctactggaa
     5101 cagcgggatg tttctctgga aggccgccac tattctcgaa gaaattcagc agcaccagcc
     5161 gcagctggcc aaggcgattc agcgggtcca tgcgcttatg gcatcgagtg ccgagcctgc
     5221 gctggttgag gcagcctata agaaggtgcc ctcagtatcg atcgataacg gggtcatgga
     5281 gcagtcggcc aatgcagcga tgatcccggt cggattcggg tggtcggacg tgggaaattg
     5341 gagcagcctt gaagaggtcg cgccacgcga caaggccggg aacgtcgtca gcgggcgcgt
     5401 catcgatatg gacagcacca attccgtgct gtacgccgat cgccgggtcg tcgcgaccat
     5461 cgggctttcc gacatggtgg tggtcgatac gccggatgcg acgttgatct gtccgaagtc
     5521 gcgctcacaa gatgtgaagc agatggttga gatccttaaa cagcagggag ctcctgaaca
     5581 cttggagcac ttgaccgtgt ttcgtccgtg gggatcctac acggtcttgg aagaaggacc
     5641 tggatataag gtcaagcggg tgacggtgaa tcccggaggg cgcctgtcgc tgcaactcca
     5701 tcataagcgc agtgagcact gggtcgtcat cgccgggact gcgcgggtca cgaggggaga
     5761 cgagctcctg gatctgcggg tagggcagag cacagccatc ccggtcgaga cgcctcatcg
     5821 ccttgaaaat ctcggctcgg agacgttgca tatcatcgaa gttcagaacg gcccctacct
     5881 cggggaggat gacatcgtgc gttttcagga cgcctacggc cgaacctccg tacggtgacg
     5941 ggtgactgga aaattggtga cgtacgtgcc gtgaagaatc gtcagatcac cacatcgaac
     6001 ctcggactcg gagtaatgca tggcactgtt tcgtgaatat gatttgagag gcattgtcgg
     6061 agaagagctt acggaagcga tcgccgagca ggtggggcgc gcgtatgcca ccatggcgcg
     6121 ggaacagggc gcctcgcgaa tcagcctcgg ccgcgacggg cgattgagtt cgcccggctt
     6181 gcaggaggcc ttactccgag ggctgctggc aggcggcctg gatgtggtcg acttggggct
     6241 gtgcgcttcg ccgctcctgt acttctcatt gttcaccttg ccggtggacg gcggcatcat
     6301 gatcaccggc agccacaatg ccgctgaata caacggcttt aagatctgca tcgggaaaga
     6361 agcgattcac ggggaagaga ttcagcggtt gcggcgggtc atggaggccg gtcaatttgc
     6421 caccggttcc ggacgactct cctctcatcc catcatcccg gactacttgg agcatctcaa
     6481 aacgaatttt tccggcgtgc gggcggacca tctccatgtt gtgatcgatt gcggtaatgg
     6541 cgcggcctcg ttggtggcca agcaggccct tgagcagatg gggtgtcgcg tgaccggcct
     6601 ctatgacgag ttggacgggc gatttcccaa tcaccatcct gatcccaccg tcgtggaaaa
     6661 tctgcaggat ctgatcgcaa cggtgcggga gaaaaaggcg gatgtcggca tcggctacga
     6721 cggcgatgca gaccggatcg gcgcgatcga cgagcgggga gagattcttt ggggggatcg
     6781 cctcatgatt gtctatgcgc gggatatcct gacccagcgc cctggcacca cctttatctc
     6841 cgaggtgaag gcttcgcaat gcctgtacga cgacattgcc gccaagggcg ggcgtccgat
     6901 catgtggaag accggtcact ccttgatgaa ggccaagctg aaagccgagt ccgcggttct
     6961 ggccggtgaa atgtccggcc atatgttttt tgccgatcgg tatttcggtt tcgacgatgc
     7021 catctatgcg tcctgccgct tggttgagat tctggccaag accaagcagt cgctctccag
     7081 tttggtcgcc gaccttcccc agaccacggt gacgccggag attcgggtcg actgcccgga
     7141 tacgctgaag tttcaactgg tcgatcaggt gcgcacgcaa ttggccgcct atctcgctga
     7201 aggcaagccc gtcggcgcgt cgaatcttcg catgcgcgag ttggtgacga tcgacggggt
     7261 gcgcgcgatt ttcgaagacg ggtggggctt gattcgagcc tccaataccc agcctgcgtt
     7321 ggtgctccgc ttcgaggctc cgtcgccggc ccggctggat gtgattcgcg cgacgattga
     7381 aaccgaactc gcccaggcac gacgtgttct gtcggcgtag tgtgctctgc tcctccgact
     7441 ccttccgtct cagcctgttc cttgccgtca cgatcctggc gacagtcggt gcctccgccc
     7501 cgttgctctc ttacgcaaca ccggatgggc agatcctcgc tcctgccgcg ttgccctttg
     7561 caaacggcga gcggttgtct tacgacgtga cctggttggg catgcgtgcg ggaatcgcga
     7621 ccatgatcgt tcaggagggc ccggtcgagg gggccaggcc gcagctcact cttgccatga
     7681 cggctcgatc gagcccgacc gttaccaagt tttatcccgt cgataaccgc ggggtgtctg
     7741 tggtcgatcg cgaatccttc ctgccccggc acatgacgtt tgccaggagg gagggcaagc
     7801 ggttcaacga tttcgactat acgttccgcc acaccgaagg gctggttact gccgtcaaag
     7861 acgggaaaag cgacgaattg ccgatccctt ccgatgcgca ggacgccatg tcgtgcctgt
     7921 attatgtgcg caaggtgctg cctttcgtgc cgggggcgtc gctggcaatg acggtgcatc
     7981 acgacaagaa gaactacaag atggacgtgc gggtcgaagc ccttgaaaca gtggagggcg
     8041 tgtggggaaa gcggcagacg gcccgggtgg tggtcatcat gccgtttcag ggcattttcc
     8101 tgaacgaggg caacattcgc gtctggttca cgaacgatga gcatcgggtg cccgtcagaa
     8161 tgaaggccaa ggtcgtggta gggtcgattg tggccgaatt gaccgacggg taccgaccga
     8221 cctcacaacc gtaacctgcc tgccttggtt tgctcgtccg gacggaaacc gctataatca
     8281 cgccgagttc gtggggaggg cttcggctct gtccgaagcc ctcttcattt cgaccggtcg
     8341 tcgttcagtt cacgaggaac cctgagcatg gcgaaatttc ccattacatt aagccgcttt
     8401 attatcgaac aacaggccgc gcatcccgaa gcgaccggag aattctctgt cctgctgacc
     8461 caaatcggcc tcgtcggaaa aatgattgct catgacttac gcagagccgg attgaacaag
     8521 atccttggca ccaccgggga gaccaatatc caaggcgagg tggtcaaaaa gctcgatcag
     8581 attgccaatg atacctttgt tcgcgtgttc gaaagcagcg gtctcgtgtg tgtcttggcg
     8641 tctgaagaaa tggaaaaacc tttgaaaatg gttcatgaag gagccaagta catgctcctg
     8701 gtcgatccgc tggacggatc ctccaatacc gatgtgaata tgccgttggg ggccattttc
     8761 tccatccgaa gatccaagct ggggcaacgg ctgcctgtgg aggacgagtt gctgcaacgg
     8821 gggaccgaac agatcgccgc agggtacgtg ttgtatgggg cgagcaccat gcttgtgttc
     8881 acggtcggtc agggcgtgca tgggttcact ctcgagccgt ccatcggtga atatctcctg
     8941 tccaacgaga acattcgaat tccctcgagg ggcaaagtct attcggtcaa tgagggcaac
     9001 taccatcgtt ggccttccgg cacacagaaa tatctcgatt cgttgaaagt gcaggacaag
     9061 gcgaccgggc gtccctatag
//
LOCUS       DAORQN010000054         8972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_404_length_8972_cov_12.518560, whole genome shotgun sequence.
ACCESSION   DAORQN010000054 DAORQN010000000
VERSION     DAORQN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_8972_cov_12.518560"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..859)
                     /locus_tag="PK614_04945"
     CDS             complement(404..859)
                     /locus_tag="PK614_04945"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRB81590.1"
                     /translation="MRVWRVTRRAHAAPDGEGARLYGGRWNLPNTPVVYASASLSLAV
                     LEYLVHVDRDLAPSDLVSIAATIPISLPVETIEIGLLPKGWQAPSDQESLQRLGSEWV
                     RAQTSAVLRVPSALIPVEFNYLLNPAHSDFGRIIWADPVPFSLDPRFLQ"
     gene            complement(859..1293)
                     /locus_tag="PK614_04950"
     CDS             complement(859..1293)
                     /locus_tag="PK614_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRB81591.1"
                     /translation="MPTHDALINMLGGPTVLKKQLRSTEDITSRIREGMPFAALSAMA
                     SHAQLDIPRLGEILMITKRTLARRRKARRLSADESDRLVRLARIFALAVEVLGDEGKA
                     AQWLQRANRALGNTSPLDLLDTDVGTQAVAKVLGRMDYGVIS"
     gene            complement(1885..3654)
                     /locus_tag="PK614_04955"
     CDS             complement(1885..3654)
                     /locus_tag="PK614_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81592.1"
                     /translation="MPPEKLDPKGPKFKQFSSEWSKILNEEAWKAIEASAENTELVVS
                     GKEVIDHHRRFFRGKSGKRLKKLVEDAAREVDLNPGLLGTIMMAETRRPQSYLSSEKV
                     RSYHIGVDDFYENRVAIKARVSAFVKVGWDRNQTPVVHDNDSKACKKKAAAQVAGDKV
                     LFDKYSKLCRQVKTILFDSGPDGVLATAVSLKFFEVRLREVATAKLNGEFDKLPLATR
                     FALTRMAFTSGTGGATPFLKDALKGIDIFVRKAIPVQAYQKQRNATVRTAQAMHLSDW
                     IFGIPVPVAAVQPELEIFESFDEHEFEDEDFEEEFEANAKAIYEDIDHELPEEIEHPD
                     YETEADEATDGIETRELDPVLLDVAEKIIAREVPVVEHEKPTRWTYCFSARDIAKVQK
                     VYEDNASAASSNSVDRCSCIVMLNVALGQLLPLRLKQNRARGTSDRHVQMGDLTTGTI
                     EKAMKQLHRKGYTDASTMMDFLDRRGRTAGTLKPERLKAGVRDKVLANSKTEGCWFAF
                     GMSIMDGYHSVLLLVDHTAADAKIYWLDQFSTGLDDDVTDSLDQRLTDKTQGWWQAVM
                     GIKRKGYNTTICLWPLRKPRETS"
     gene            3661..3864
                     /locus_tag="PK614_04960"
     CDS             3661..3864
                     /locus_tag="PK614_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81593.1"
                     /translation="MPGKEKEVCQFVLISPACLYGEFQGKRPVSLTKRIGNVFGDSRW
                     IKLLFQGVFVELFKFILSVIAAG"
     gene            complement(3861..5567)
                     /locus_tag="PK614_04965"
     CDS             complement(3861..5567)
                     /locus_tag="PK614_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81594.1"
                     /translation="MTSRDEKDGALRSHPQGALESPFLDEKLFSDASNVERNAHVAVL
                     ETESPFRSAFEEGSTSPIVPEEFEEEFVQAYDEETPPADMEDSQKEIYYEEEAEYEEE
                     EAGEEKALARMKCEFQTSNKIWRNDGKTSSVLPRKYGPNDFLFKNEKKGVRLEAETDG
                     VLEFETEWFRTWPKLKEAIEEAVKMTDDMNRAASAKYEKTRKAFPFNVDHLRTGSANE
                     INKGYWNPASYPRYENRHWNIDSTKYEHERILRAGEDLEVEIIDPKWKAAIQTSESFF
                     LEYYESFLRQHERLSLVEGTIKHAQAILDAANTRGIPVTELVKLRSFLQIIVNYIMRG
                     RSVDLKGQPSKFAFRLMSRTNFASMYESLLTPKEKEMFKEIVKNETILNKELKEMGLD
                     RNDRLFKNGYGTEGHCGPTIGEWLAGLVRGVDLLSVRSEKGLSAAMGRYNVEKKKGKK
                     DQWHVKFETRGTRIGRVREAKDWEDYAHKLFDLASKRERDALNLLHQQGIVDENKLTN
                     FVFHARHPELGGRRIRKDEQALAQEWQQIRDDLVRPHLQGVQPELQEFEGTGGSWKER
                     RN"
     gene            complement(5586..6647)
                     /locus_tag="PK614_04970"
     CDS             complement(5586..6647)
                     /locus_tag="PK614_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81595.1"
                     /translation="MLEAAWQFRAHDPDRPLGHPGHRSNLPQLPDFWLDLIRDSAVPF
                     IVPPPGAQGTVETTLAPALVLGQKNVLWDHLIYAYMIENTRIYEIFRRILHEFLHGEK
                     LGVPTPAAQHWLRNTEELFYRDPPSFSITTITSHIRSDLGALRRHAYHRMFAMDLNHG
                     TDEGQPYPYFKADAANNEFVTTFEEFLREAWVAISNRHNTSGTNPTDQGKIENLLANL
                     QNMLMSRRANGNLSREEFVFVSMMSWFHLTVEFDSPIIQALRAEATGREQRLFKVAQR
                     VGLPAHGLSKSYFDIADPISRVLIDVEASGTAIVPDIVATAAPPTPPNAIQVALNTII
                     THWSIITGRDVKARKVTTT"
     gene            complement(6729..7079)
                     /locus_tag="PK614_04975"
     CDS             complement(6729..7079)
                     /locus_tag="PK614_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81596.1"
                     /translation="MTEPTTEQGTAVEEFRQISNILAVPTIGNPLPDGRKWKLPCPDK
                     KALADHPAGPLDDEVKNCLAETSSRSAESITGQSAVQTSAHPAPPQTQEPQTSETSIK
                     QTSESGSSKQSGKR"
     gene            complement(7279..8367)
                     /locus_tag="PK614_04980"
     CDS             complement(7279..8367)
                     /locus_tag="PK614_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81597.1"
                     /translation="MNDSHVAIKTQLARQVAHWGDAVARVQELDDLASPAAWNGLERY
                     LGLSIRQHLSTVAAQLSREATLLQDALASALSVADLSAVRRQLLTLRTRYMRVETTLD
                     FFADAINTRTTPKIAGYLRACDTLAHRSMAQLLDQLGKPTPVALTYLDKGLGASILKA
                     RLRLWDGTSLNPVAAIKITRHNLHRPTALIHEAGHQVAHIVGWNEELASSLAAGLTDA
                     PAGVGEAWASWASEIAADAFAFVHTGYASVAALHDVLDGGPSMVLRHIPGDPHPISYL
                     RVLLGVEMCRQFYGAGPWDDLAEAWTRQYPLERVPDPTGTLLRASVPMLPRVVEATLR
                     QPMRAFGGRPLVALINPDRVKAETLLFM"
     gene            complement(8360..>8972)
                     /locus_tag="PK614_04985"
     CDS             complement(8360..>8972)
                     /locus_tag="PK614_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81598.1"
                     /translation="GQRLYYFEIPGKHPLTVPGPVGQAQVRRPTRARLILDFPKNEGS
                     IYLFLSEIRAQEIAVKLRQHAHIGVVMARLRRIIDRGLGKAFAGKFGRLKIIHEAVTP
                     DHWLGALRRLPSLVPQVLRGRLTEWAVKGLSDHLKQRTEELIKAAEDTADGVTLVITL
                     GNLPGFPQLRQALKGKGFPLASLKLSDGMPTVKVKVVPGYSHE"
BASE COUNT         1834 a         2583 c         2460 g         2095 t
ORIGIN      
        1 gccaggccgc cccgctatga gaaaggaaga gcaaaggaat caggaggaaa cggctggtcc
       61 cccttcgccg acactgtcag ctcgccaggc tcgaagagga tgatccggaa ttgttcttgc
      121 tcgcattacg gaatgtgctg gagggacaaa gatgggttgc atcgctcgcc gagagaaact
      181 aactgaatcg agacagtctg tatcgcatac tctcggagcg cggcaatccg gaatttcgaa
      241 gcctcgatgc cctcctccac gcgctaggct ttcggctcgc ggttgccgtc agtcgctaga
      301 cgcagatcac tgggcatgtg aatgaactcc tgtggccaac agaaagacat cccggatgtt
      361 cgttacaaaa cctgcccttt tttccaggcg cctgggtgct cgcctactgc agaaaccggg
      421 ggtcgagcga aaagggcacc ggatcagccc agatgatgcg tccgaaatcc gaatgggcag
      481 gattgagcag gtaattgaat tcgacaggaa tcagcgccga cggcactcgc aggaccgcgc
      541 tcgtttgtgc tcgaacccat tcgcttccga gccgttggag cgattcctgg tcagacggag
      601 cttgccaacc cttcggcaat agcccgatct ctatcgtctc aaccggaagg ctgatgggaa
      661 ttgtcgcggc gatggagacg agatccgatg gagcgagatc gcgatccaca tgcacgaggt
      721 attcaagcac ggctaacgac aatgaggcgg aggcatagac caccggcgtg ttcggcaggt
      781 tccagcggcc gccgtagagg cgagcgcctt cgccgtccgg cgcggcatgc gcgcggcgag
      841 tgacgcgcca cacccgcatc aactgatgac gccgtagtcc atccggccga gcaccttcgc
      901 caccgcttgg gtccccacat ccgtgtccaa cagatcgagc ggcgaggtat tcccgagcgc
      961 ccgattggcc cgctgcaacc actgagcggc tttcccctcg tcccccaaca cttccacggc
     1021 cagcgcaaaa atgcgagcca ggcgcaccaa tcgatccgat tcgtccgctg acagtcgccg
     1081 ggccttccgc cgccgcgcca acgtccgctt ggtaatcata aggatctcgc ccagccgcgg
     1141 aatgtcgagt tgggcatggc tcgccatggc cgagagcgcc gcgaaaggca tgccttcacg
     1201 aatgcgcgag gttatgtctt ccgtggagcg aagctgtttc ttcagaaccg tcggcccgcc
     1261 cagcatgttg atcagcgcat catgtgtcgg catgtcgctc tccccgcctg tgccacatgg
     1321 ctgcagtata atgccaaatg gcatcctggt caagaaacga tgggggccgg tggaagcctg
     1381 gtgcgggaca gccaaaccgg gaggaacccc gggaagcgga accgaatgca ggaacgaata
     1441 gcctggcaga ctgcagcagt cctacgtcgc cgcctccccg cctgcctgtc acgagtcagg
     1501 gcgctccaaa cgattcgatc caaacggaat agcagtatgt cgacggcaag ccgcatccgg
     1561 cacatcgact cagtgctgca ggactcggat actgcgccag gatcaaggac agtcggcagg
     1621 cgtgaatcag actcgactgt gcatgatctc gatggaggca tgtcgtcctg gcgaatcgcc
     1681 gttgtccatg cagaatggca atgtgggagt gcaagacgcg accgcatttg tccagatggc
     1741 tgagcggttc gggatggacg caacgctacg acctcgtggc cgaccgaaag gatcttgaaa
     1801 caaagactcc cgcccccttt tctttccccc agcatcgtta acgacaaacc agccatcgtg
     1861 tttggtttat aggtcagcac accgttaact cgtctcccga ggcttcctga gtggccagag
     1921 gcagatcgtt gtattgtagc ccttgcgctt gatgcccatg acggcctgcc accacccttg
     1981 tgtcttatcg gtgagcctct ggtcgaggct gtctgtaacg tcgtcgtcca ggccggtaga
     2041 gaactggtcg agccagtaga tcttggcatc ggcagcggtg tggtccacca acaggagcac
     2101 gctgtgatat ccgtccatta tcgacatgcc aaaggcgaac caacaccctt cggtcttgga
     2161 attggccaag actttgtctc gaacaccggc cttgagccgt tcgggtttca gcgttccggc
     2221 cgtacggcct cggcggtcaa gaaagtccat catcgttgag gcgtcggtgt agccctttcg
     2281 gtggagctgc ttcatcgcct tttcgatggt tccggtggtg agatcgccca tctgcacgtg
     2341 tcgatcgctc gttccgcgcg cgcgattctg cttgagccgc agtggcagta gctgcccgag
     2401 cgctacgttc agcatcacaa tacaactaca gcgatcgaca ctgttggagc ttgccgccga
     2461 cgcgttgtct tcgtagacct tctgtacttt tgcaatgtcc cttgccgaaa agcagtacgt
     2521 ccaacgggtg ggcttctcgt gttcgacgac aggaacctcg cgtgcgatga tcttctccgc
     2581 gacatccaac aacacggggt cgagctcgcg ggtttcgatg ccgtcggtcg cctcgtcagc
     2641 ttccgtttca taatcgggat gctcaatctc ctccggaagt tcatggtcta tgtcttcgta
     2701 aattgctttt gcgttggctt cgaactcttc ttcgaaatct tcatcctcga actcgtgctc
     2761 gtcaaagctc tcgaatattt cgagttcggg ctgcaccgcg gcgacgggca ctgggatacc
     2821 gaagatccag tcagagaggt gcattgcctg ggcggtccgg acagtggcgt ttcgttgttt
     2881 ttgataagct tgaacaggga tggctttacg gacgaagatg tcgatgcctt tcagcgcatc
     2941 cttgaggaaa ggtgtggcac cacccgttcc actggtaaac gccatccttg tgagggcaaa
     3001 tctggttgct agcggcagct tatcgaactc tccgttcaat ttggcagtgg ctacctcacg
     3061 aagacgaacc tcaaaaaatt taagactaac tgcggtcgcg agcactccat caggaccgga
     3121 atcaaacagg atagttttga cttgcctaca aagtttcgag tatttgtcga acaggacctt
     3181 gtcgccggcg acttgcgcag cagctttctt tttgcaggct tttgagtcgt tatcgtgtac
     3241 cactggagtt tgatttctat cccatccgac cttaacgaag gccgaaaccc tggcctttat
     3301 agcgacgcga ttttcgtaaa agtcgtcaac gccgatatga taggaacgga ccttctcact
     3361 tgagaggtaa ctttgtggcc tgcgtgtttc agccatcatg attgtcccta gcagaccggg
     3421 attgaggtcg acctctctag ccgcgtcttc gactagcttc tttaatcttt tcccactctt
     3481 tccccgaaag aacctccggt gatggtcgat aacctctttg cccgacacaa caagctcggt
     3541 gttctccgcg gatgcttcaa tggctttcca agcttcttca ttgaggattt tgctccattc
     3601 agagctgaac tgtttgaact tcgggccttt cgggtctaat ttctcaggtg gcaattccgg
     3661 atgccgggca aagaaaagga ggtttgtcag ttcgttctca tctcgccagc ctgcttgtat
     3721 ggcgagtttc agggcaagag gccagtctct cttaccaagc gcatcggcaa cgtctttggg
     3781 gatagccgat ggatcaaact gctcttccag ggggtgttcg tagagctgtt caagttcatt
     3841 ctttccgtaa tcgctgccgg ttaattcctc cgttctttcc agctcccccc ggtcccctca
     3901 aactcttgca gttcgggttg aacaccttga aggtgcggtc gcaccaaatc gtctcgaatc
     3961 tgctgccact cttgggcgag agcctgttca tccttcctga tccgacgacc tcccagctcc
     4021 ggatggcgcg cgtggaagac aaaattcgtc agtttatttt cgtcgacaat cccctgttga
     4081 tggaggaggt tgagggcatc acgctccctc ttgctcgcca ggtcgaatag tttatgtgca
     4141 tagtcttccc aatccttggc ttctctcact cttcctattc tcgtaccgcg agtttcgaac
     4201 ttcacatgcc attggtcctt ctttcccttt ttcttttcca cgttatatcg gcccatggct
     4261 gcgctcaggc ctttctcgct tcgtactgac agcaaatcca cacccctgac caaccctgcc
     4321 agccattcgc ctatggtggg gccacaatgt ccctccgttc catacccgtt cttgaacagc
     4381 cgatcgttcc gatccaggcc catttccttg agttccttgt tgagaatcgt ctcattcttg
     4441 acaatttcct tgaacatctc cttttctttt ggtgtcagca aactctcata catcgacgca
     4501 aagttggtgc gactcatcaa ccgaaaggca aacttggaag gttgcccttt gagatcaaca
     4561 ctcctccctc gcatgatgta attgacgatt atttgtagaa agctgcgcag cttgaccaat
     4621 tccgttacgg gtatgccacg ggtattcgcg gcatccagaa tcgcctgtgc gtgcttgatg
     4681 gtgccttcca cgagagatag ccgctcgtgc tgtcttagga aggattcata gtactccaga
     4741 aagaagcttt cagaagtttg aatggcagcc ttccacttgg gatcgattat ttcgacttcc
     4801 aagtcctctc ccgctctaag aattcgttca tgctcgtatt ttgtactgtc aatattccag
     4861 tgccggtttt catatctggg gtagctggcg gggttccagt agcctttgtt tatctcatta
     4921 gctgatccag ttctcagatg atcaacgtta aacgggaatg ccttacgcgt cttctcgtat
     4981 tttgccgaag cagccctgtt catgtcgtcg gtcatcttaa ccgcttcttc tatcgcttct
     5041 ttgagtttag gccaggtacg aaaccactcc gtctcaaatt ctagaacccc atccgtctca
     5101 gcctccagcc tcactccttt cttttcgttc ttgaagagga aatcattggg accgtattta
     5161 cggggaagca cgctggaggt cttgccatca tttcgccaga tcttgttcga ggtttggaac
     5221 tcacacttca tccgagccag ggccttttct tcacctgcct cttcttcctc gtattcggct
     5281 tcctcttcgt agtagatttc cttctggctg tcttccatat ccgccggcgg cgtctcctcg
     5341 tcgtaagctt gaacgaactc ctcttcaaac tcctccggca ctatggggct agtgctcccc
     5401 tcctcgaagg cactccggaa cgggctctcc gtctccaaca ctgccacatg cgcattccgc
     5461 tcaacgttgg acgcatcgct gaacagtttc tcatccagga agggagactc aagtgcaccc
     5521 tggggatgag aacgcaacgc cccgtccttt tcatctcgtg aggtcatagc tgatcacctc
     5581 aggcattatg tcgtcgttac tttcctggcc ttcacatcgc gaccggtgat gatcgaccaa
     5641 tgggtgatga tggtattcag cgccacctga attgcattgg gaggcgtggg gggcgcagca
     5701 gtagctacga tgtccggcac aatggccgtt ccgctggctt cgacatcgat aagaacccga
     5761 gagatcggat cggcgatatc aaagtagctc ttagaaaggc cgtgcgccgg caggcccact
     5821 cgctgagcca ccttaaagag ccgttgttcc cgaccggtcg cctcggctcg gagggcttga
     5881 atgatgggcg agtcaaactc cacggtcagg tggaaccagg acatcatgga gacgaagaca
     5941 aactcctccc gtgacagatt gccgtttgcc ctgcgagaca tcagcatgtt ctgcagattc
     6001 gccaggaggt tttcgatctt gccctggtca gttgggttgg ttcctgaggt attgtggcgg
     6061 ttactgatag cgacccaggc ctcacggagg aattcctcaa atgttgtcac aaactcgtta
     6121 ttggcagcat cggccttgaa atagggatag ggctggccct cgtcggtgcc atggttcagg
     6181 tccatggcga acatccggtg ataagcgtgc cgccgcaagg cccctaagtc cgagcggatg
     6241 tggctagtga tggtggtgat agaaaacgac gggggatctc ggtaaaacaa ttcctcggtg
     6301 ttgcgcaacc agtgctgtgc agcaggcgtt ggaacgccca gcttttcacc gtgcaggaac
     6361 tcgtgcagaa tacgccggaa gatttcatag attcgggtgt tctcgatcat gtaagcgtag
     6421 atcagatggt cccaaagaac atttttctga ccaagcacca aagcaggggc caaagttgtt
     6481 tctactgtgc cctgggcgcc agggggagga acaatgaaag ggacagcgga atctcttatc
     6541 aggtcaagcc agaaatccgg cagttgaggg aggttgcttc tatggccagg gtgccccagt
     6601 ggtcgatcgg gatcgtgagc tcgaaattgc caggccgcct ccagcaaagc tgtcagcacc
     6661 gaagggtgct ggagaaacag cgtattggcg cctccgggaa tactgtttgc gtatcgtctg
     6721 aacatgtttc acctcttccc actctgtttt gatgaaccac tctcggaagt ctgtttgatt
     6781 gaggtctcgg aagtttgagg ctcctgtgtt tgcggcgggg ctggatgagc agaagtttgt
     6841 acggctgact ggccggttat gctctctgcg gatcgagagg aagtttctgc aaggcagttt
     6901 tttacctcgt catccaacgg ccccgccgga tgatctgcaa gcgccttctt gtccggacaa
     6961 ggaagcttcc atttcctacc gtcgggcagt ggattcccaa ttgtgggaac agccaagata
     7021 ttgctgatct gcctgaattc ctccacggca gttccctgtt ctgttgtcgg ttctgtcatg
     7081 attaaatcct ccttcgttat ggatttaagc cgcccgcaaa gcaccgccaa gtcgcaacat
     7141 ccattgctcc tgttgcttga acgtctccgc ctttctctca gccatggtcg ccgcacggaa
     7201 accggttagc gccagcagcc gcagcgcttc ggtccaaatc cagtgcattg aggtgtagag
     7261 cgcaggacct agttgccgct acataaaaag aagcgtttcc gccttgacgc ggtcggggtt
     7321 gatgagggcg accagggggc gtccgccgaa tgcccgcatg ggctgacgga gcgtggcctc
     7381 gacgacgcgt gggagcatcg gcacagacgc ccgcagcaac gtccccgttg ggtctggcac
     7441 cctttcaagg ggatactgtc tggtccaggc ctcagccagg tcgtcccagg gaccggcgcc
     7501 ataaaactgc cggcacattt ccaccccgag caatacccga agatagctga tgggatgggg
     7561 gtcgccagga atgtgccgaa ggaccatgga ggggccaccg tccaagacgt cgtgcaaggc
     7621 ggccacgctc gcgtacccgg tatggacgaa agcgaaagca tcggctgcta tttcggaagc
     7681 ccaacttgcc caggcttcgc caacgccggc tggcgcatcg gttaggccgg ctgcaaggct
     7741 ggaggcaagt tcttcattcc agcccacaat gtgcgcaact tgatggcccg cctcatgaat
     7801 tagggccgtg ggccgatgca agttgtgccg ggtgatcttg atggccgcga cgggattcag
     7861 actggtgcca tcccacagcc gtaacctggc tttcaggatc gaggctccca accccttatc
     7921 aagataggtg agcgcgaccg gcgttggttt tccgagctgg tcgagcaatt gggccatact
     7981 ccggtgcgcc aacgtgtcac aggctctcag gtatccggcg atcttggggg tcgtgcgtgt
     8041 attgatcgcg tcggcaaaaa aatccaacgt ggtctccact cgcatgtagc gtgtgcgaag
     8101 ggtcagcaat tgccggcgca cggcggagag atcggcaacc gacagggccg aagccagcgc
     8161 atcctgcaac agggtcgcct cgcggctcaa ctgagcggcc acggtcgaga ggtgctgtcg
     8221 aatggacaac ccaaggtagc gctcgaggcc gttccatgcc gcaggcgagg ccagatcgtc
     8281 cagttcctgg accctcgcca cggcatcccc ccagtgggcg acctggcggg caagctgcgt
     8341 cttaatcgcg acgtgcgaat cattcatggc tgtaccccgg gacgaccttg accttgactg
     8401 tgggcatgcc atccgacaac ttcaggctag caagggggaa ccctttccct ttgagagcct
     8461 gacgaagttg cgggaagcct ggcagattcc cgagcgtaat cactagcgtc acgccatcag
     8521 cggtatcctc cgcagccttg atcaactcct cggtgcgttg ctttagatga tcggatagtc
     8581 ccttgaccgc ccattccgtc agtcggcccc ggaggacctg cggaaccaga gagggcaatc
     8641 ggcgaagggc acccaaccaa tggtcgggcg tcacggcctc atgaatgatc ttcagccgtc
     8701 caaattttcc ggcaaatgcc ttgcccagcc cacggtcgat gatcctgcgc aagcgcgcca
     8761 taaccacgcc gatgtgcgca tgctggcgaa gtttcacggc gatctcctgt gcgcggattt
     8821 cgctcagaaa caggtaaatg ctgccctcgt tctttggaaa gtcgagaatc agccgagccc
     8881 gggtggggcg acgcacttga gcttgaccga ccggccctgg aaccgtaagc ggatgcttcc
     8941 ccggaatctc aaagtagtac aatcgttgcc ca
//
LOCUS       DAORQN010000055         8929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_408_length_8929_cov_27.605815, whole genome shotgun sequence.
ACCESSION   DAORQN010000055 DAORQN010000000
VERSION     DAORQN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_8929_cov_27.605815"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="PK614_04990"
     CDS             complement(<1..1918)
                     /locus_tag="PK614_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRB81599.1"
                     /translation="MPDSTMMPGWLKQRMINRRVSLSQWNSVTVCGLVLLTMLTVIAE
                     VNAASAGSNRSQSTEIQKPTIYPQLGHSALPLDAKLSPDGRRVISLGGDGVLRVWDRQ
                     TSRELYLIMASDHLDGELESKKRLFVNRDGRQVAVRGVKGERDLLTIVSVASGEIVRE
                     IWLDHNDMKESKVKDLDVSPDFSTVAAAYENKPGLVLYSMSSGGIEQRFEGYELMEVA
                     FIPDGRIASRTVGKIIIWNPKAGGAPQMLDTGRNKETFGNDARPGLALCDHWLLYHGE
                     NELVAFDWQTGKMAQKWKGLGLAFLSRPTGVCSRNTPDAYLAPYEPDLEVLKNSRPIR
                     KDIVTGNQRSLIEIADVGIPAIYDQSVDGRFLVVTPVVPLDIENSESRDRFEFANKDL
                     VIVDTVTGRVVQRLTGGIPAVERVFAFQSGGRALSTGKDHRINLWNLEHGRVEWSLEW
                     KRPVQCVGALAGERRFAVLGSEEGKPNFAIFEMNTGKVESEWTFKGKWAMRACTVVPG
                     RSEAVVVVREESESDESDRPWEIYQWNLQTGQLVLQYQINAGNYPHVDVSSDGKWTAW
                     SQKGHGVTIFNGPGEGRVIAHGLMDKKGEREELRLVQFSADGQRLLIVGWNGQAEIWD
                     IARQSLVAAFPAEAK"
     gene            2415..2759
                     /locus_tag="PK614_04995"
     CDS             2415..2759
                     /locus_tag="PK614_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81600.1"
                     /translation="MLIRAIALSVSLAILLLPLAGQAADYRFVDRGDTYYLDHLFENQ
                     LVVVLGKENGWVKVRYLNGAVEWVAPNRLMTQSQSRANDTAEQVVGTGVFLLALYCLA
                     NPAACSDSPSTR"
     gene            complement(2949..3392)
                     /locus_tag="PK614_05000"
     CDS             complement(2949..3392)
                     /locus_tag="PK614_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81601.1"
                     /translation="MSRLARAKEEEPMAPAWSVTGFTVCCILYVLGVFSAPSAQAGGC
                     EKWKSEFLSGAMTLTSYINAHHKYFQKKLTSVEFETQALNLVAKLTALEGRVKAVASC
                     VHPEDKFEGRRRFLLQTLAVVIPLMKAGDVVAPEIRDRLEREGHD"
     gene            complement(3400..6207)
                     /locus_tag="PK614_05005"
     CDS             complement(3400..6207)
                     /locus_tag="PK614_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB81602.1"
                     /translation="MKASRPVSLVIGFAGTILLALLMLLVPGLTESWEAKTFDLRSQW
                     HRATPDSGRLLLIGRDAESDARFGVGIWDRAVFATMISGLHRAGAQVVGLDFHFGEES
                     PAERGGAGSDRALVEAATRAGNVLFPLPVNLSDHAGEAPTVPLPLRNGMARSLPSLEP
                     AVLHALPTTDHIAGPLSGLLQAARGVGHIGAISDADGVYRRVPSFVNAGGQAIPSLGV
                     ALAAAYLHITPDRMALSPGRSLTFQEATFSDGTKRNLSIPVDEHGDILINYAGRWTEG
                     PFPYLSFVDVWDAIQEGRDAELREQVAGKAILIVHAALGSDKRQTPLELTAPGGFILA
                     NIFNTILTESALWTVPVWLHWVLVMGIGAGAAWCLLAFSLWGGLVAVALTGAVYLLVS
                     FAGFASSGLVLPVVLPFMALALATGSTLVWTRWIGARHIRQLEDERVEYYRRLADTQR
                     ALVERETRAEQLEEDLNQAQADARVGQTEGTELQALVAALQRDLQTAQAQASQARDAV
                     NDLQFRLDKMQTAQANRSPLLEGELDVLRQDCAQFGILTRDAVLLRMWKDLKKVAKSH
                     VPILILGEPGTGKELFAQAGHRLSERASYAFVPVNMAAVPADLFESELFGHLRGSFTG
                     AVRDHEGYFQQADKGTIFLDEIGDLRSDLQAKLLRVLQDGMVMRVGDKKAMKVDVRVV
                     SATNRNLLQGIAEGWFREDLYYRLHGIELRLPPLRERQTDVPALAQAFIDQFVAKAGR
                     GPVALSEGAVDRLQSWPWKGNVRELKRCVENAVMLAEGATIQAEDLRLTGPGAATTAQ
                     AVPVVDVPEGEGDPKKSDSALLKTIREHSFDLQATAATLGWDRSTVMQRLKGMCFQAL
                     VQTGGDQRAAAASLAGEPGLTRLVEVKFKEYVEHLRKVVATYPSQEAALIGCRKRFKN
                     LPERYQEALATLVRLNMEGGG"
     gene            complement(6351..>8929)
                     /locus_tag="PK614_05010"
     CDS             complement(6351..>8929)
                     /locus_tag="PK614_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB81603.1"
                     /translation="WVLSGGSGTVKLWEVATGREVRSFMGHTAAVNSVALSPDGRWAL
                     SGGEDGRVKLWEVATGREVRTLERTYDVKSVAFSPDGRWALSGSVGAGLKLWAVTTGQ
                     EVRKFKGYWVPVYSMALSLDGHWALLGSSDGAVSLWEVATGREVRTFTGHKVMVTAVA
                     FSPDGRWALSGSDDGAMKLWEVATGREVRSFMGHLLSDLRSVAFSPDGQWALSGSGDQ
                     TMKLWEVATGREIRAFSGHRLAVNSVAFSPDGRWALMGSVGGAIWLWEVATGREVRTF
                     TGHSYDVRSVAFSPDGRWALSGSGDRTVKLWEVATGREVRTFTGHSSDVWSAAFSPDG
                     RWVLSGSLGGELKLWEVATGREVRSFIGHGNSVRSVALSPDGSLAVSGGMGMNVWRVA
                     SGEELLTTVLLGDREWISLTPEGYYAASPKGDNYLNVRLGNQVYGIDQFRSTFYRPQI
                     VEATLRLGNREQAIAEILGEGDKRATGGAGDFIAPPTVAISSPEDRSVLATPRLDLAV
                     TITDKSYPIQSVRVSRNGAQVWPLQTRDLVVTAKSGVEKAPVVPGTVPAGVREWTTAI
                     PLDLEEGENVIEVKAFNGHSETVKAITVSLPASLVPHDDVIKPNLWILSIGINAYQDR
                     QIRALSYAEADAQAIVQAFTQQRGKLFRDVRSLIISDSSTMKPTYDTILDNLGYLRQA
                     GQNDVVLLFLAGHGINDDRGEFYFLPSDAAIQSDGSIKRSRAISNDEIKKVLDLPAKK
                     IVFLDACHSEGVSKKKTRGVDNNRFIKELQAMDAVILTSSAGNELSQEGDAWKHGAFT
                     YALLEGLSGKADLITKDGIVSMKELDGYVSERVPHLTNGAQHPITDTPGGYRNFPVAL
                     LR"
BASE COUNT         1933 a         2942 c         2196 g         1858 t
ORIGIN      
        1 ctttggcctc tgcggggaac gctgccacta atgattgccg agcaatgtcc cagatctctg
       61 cctgcccatt ccatcccact atcaataagc gttgtccatc ggcggagaac tggaccagac
      121 ggagctcttc tcgttcccct ttcttgtcca tcaacccatg agcaatcacc ctcccctccc
      181 caggcccgtt aaaaatggtc accccatgtc ccttttgaga ccaagctgtc cactttccat
      241 ctgaagaaac gtccacgtgg ggataattcc cagcattgat ctgatactgc aaaaccagct
      301 gaccggtttg taaattccat tggtagatct cccatgggcg atcagattca tcagattcac
      361 tctcttctct cacaacaacc actgcttcgg agcggccagg aacgaccgta cacgctctca
      421 ttgcccactt tcctttaaac gtccactctg attccacctt gccggtgttc atttcgaaaa
      481 tggcaaaatt aggctttccc tcttcagatc ccaataccgc gaatcgtcgc tctcccgcca
      541 aggcacccac acactgcacg ggcctcttcc attcgaggct ccattcaaca cggccatgtt
      601 ccaggttcca taggttgatg cgatgatcct tccccgtcga aagggcgcgt cctccagact
      661 gaaaggcaaa tacccgctct acggctggaa tcccgcctgt caatcgctgg accacacgac
      721 cggttaccgt atcgacgatg accaagtcct tgttcgcgaa ctcaaaccga tcacggctct
      781 cgctgttttc aatgtccaaa gggacgacag gtgtgacaac gagaaaccgc ccatcaacag
      841 attggtcata gatggcagga attccaacgt cagcaatctc aatgagcgac cgctggttgc
      901 ccgtgacaat gtccttccga ataggacgtg aatttttcag gacctcaagg tcaggttcat
      961 agggcgctag gtaggcatct ggtgtgtttc gtgagcagac gccagtgggg cgcgagagaa
     1021 aagcgagccc gaggcccttc cacttttggg ccatctttcc tgtctgccaa tcaaacgcca
     1081 caagctcatt ttcgccgtgg tacagaagcc aatggtcaca tagggccaaa ccgggacgag
     1141 cgtcattccc gaaagtttcc ttgttccggc cggtatcaag catttgcggt gctccaccgg
     1201 ccttgggatt ccagatgatg atcttcccca ccgtgcggga cgcgattctt ccgtcaggga
     1261 taaaggcaac ctccatcaat tcgtacccct caaaccgctg ttcaatgcct ccagaggaca
     1321 tggaatacaa aacaaggccg ggcttgtttt catacgcggc cgccaccgtc gagaaatccg
     1381 gagacacgtc cagatctttg actttgctct ccttcatgtc gttgtgatcg agccaaattt
     1441 cccgaacgat ttccccactc gctacactca caatcgtgag aaggtccctc tcgcctttca
     1501 cccctcgaac tgcaacttgt ctcccgtccc gattcacaaa tagacgtttc ttgctttcca
     1561 actccccatc gagatgatca ctagccatga tcagatacag ttctctgctc gtttgacgat
     1621 cccaaaccct gagcacgcca tctcccccca acgagatgac gcgacgccca tccggcgaaa
     1681 gctttgcgtc caacggcaag gcactatgtc ctaactgcgg gtagatcgtc ggcttctgga
     1741 tctccgttga ttgcgaccta ttggacccgg cagaagcagc gttcacttca gcaatcacag
     1801 taagcatggt cagcaagacg agcccgcaca ccgtcacgct gttccattgg gaaagcgata
     1861 ccctgcggtt gatcatccgt tgttttagcc atcccggcat catcgtggag tcaggcatgg
     1921 tcaacactcc ttgcaggtac ggttgtcggg aatgtagagg gcgcataatc ggctcatttc
     1981 tgccctgtgc tcaatcgaag cggcatgctc gtatcacacc tccgataagg ccgcaatcct
     2041 cgtcggatcg cgctaccttg ttgggccggg ataatgcaaa cgctattccc tgacgcccag
     2101 catttacatg tatttctcgc tcgtgggttt tccttgagag tctgtccgac cattcaactc
     2161 cgacgttgcc cagccatgtc ggattcccct tcgactttcc ccacagctcg cggggaatcc
     2221 cacacagcac tcccagatgc cttggcccta ttgcccccgg cctgatcgcc attattcttt
     2281 tcataacagc ggtttagatg atcattgtga actgacccgc aatggcattt ctcttgctca
     2341 attcctcgct agaaattgca catggttgat cgatgttcgt tatggccatt gtaatcattc
     2401 acaaggaggg tgccatgttg atacgagcca ttgccctgtc tgtttcactt gcgatcttgc
     2461 ttttaccact ggccggccag gctgccgact atcgcttcgt cgatcggggc gacacgtact
     2521 atctggacca cctcttcgag aatcagctgg tcgtcgtcct gggcaaagag aacgggtggg
     2581 tcaaagtgcg gtatctgaac ggagccgtgg agtgggtggc accgaaccgg ctcatgactc
     2641 agagccagtc ccgcgccaac gatacggctg agcaggtagt tgggaccggc gtgttcttat
     2701 tggcgcttta ctgcctggcc aaccctgcgg cctgttcaga ttcaccatcc acacgttagg
     2761 gagagccgtc tgtgcatgaa agggcggcca aactcgttgc ttgtcccgga gggcctgtgt
     2821 gaggaatgca tctcccacag gccctccctc aggcgagcag cttagtgatc gtccctttgg
     2881 ccagccgatc ttctagacat ctcgaagata acacccccac catcgtgacc ttcacgccgc
     2941 taacctcgtc agtcatgccc ctcccgttcc agtcggtcgc gaatctccgg cgccaccaca
     3001 tcccccgcct tcataagtgg aatcacgacg gccaacgtct ggagcaggaa tcgcctccgg
     3061 ccttcgaatt tatcctctgg gtgaacgcac gacgcgacag ccttgacccg accttcaagc
     3121 gccgtcagct ttgcaacaag attgagggcc tgcgtctcaa actcgacgct ggtcagcttc
     3181 ttctgaaaat atttgtggtg cgcattaatg taggaggtca gcgtcatcgc cccactcagg
     3241 aactccgatt tccatttttc gcaaccgccg gcctgggctg atggagcaga gaacaccccc
     3301 agcacgtaca ggatgcagca caccgtgaaa ccagtcactg accatgcagg agccatcggt
     3361 tcctcttcct tcgcccgtgc cagcctactc accgaccgcc tacccgccac cctccatatt
     3421 gaggcggacc agcgtcgcca gcgcctcctg ataacgctcc ggcagattct taaaccgttt
     3481 gcggcatccg atcagtgcag cttcttgtga gggatatgtc gcgacaacct tcctcagatg
     3541 ctcgacatac tccttgaact tgacctccac tagtctggtc aatcccggtt cgcccgccag
     3601 actcgccgcc gcagctcgtt gatctcctcc cgtctgtacc aaggcttgaa agcacattcc
     3661 cttcaaccgc tgcatcaccg tactacggtc ccatcccaat gtcgctgcag tcgcttgcaa
     3721 atcgaagcta tgttcacgaa tggtcttcag caacgcggaa tcgctctttt taggatcgcc
     3781 ctctccttct ggcacatcga ccaccggtac cgcctgagca gtcgtggccg cccctggtcc
     3841 ggtcaatcgc aagtcctccg cctgaatcgt ggcgccttcc gccagcatca ccgcattctc
     3901 aacgcatcgt ttcaactccc gcacattgcc tttccagggc caactctgaa gacgatcgac
     3961 cgcgccctcg gaaagcgcaa ccggtcctcg gcccgctttg gcgacaaact gatcgataaa
     4021 ggcctgcgcc agcgccggca catcagtttg gcgttcgcgc aacggtggaa gacgcagttc
     4081 aatgccatgc aaccgataat agagatcctc ccgaaaccat ccctcggcaa tcccctgcag
     4141 aagatttcga ttggtggccg acaccacccg cacgtccact ttcatggctt tcttgtcgcc
     4201 gacccgcatg accatgccat cctgcagcac gcgaagcagc ttggcttgga ggtcggatcg
     4261 aaggtcgccg atttcatcga ggaaaatcgt gcccttgtct gcttgctgga aatagccctc
     4321 gtggtcacgg actgcccccg tgaaagatcc acgcaaatgc ccgaacagct cgctttcgaa
     4381 gaggtcagcc ggcacggctg ccatgttcac cggtacgaac gcgtaggagg cccgctcgct
     4441 caaccgatgc ccggcttggg cgaacaattc cttccccgta cccggctcgc cgaggatcag
     4501 aatgggcaca tggctcttgg caaccttctt caggtctttc cacatgcgga gcaacacggc
     4561 atcccgcgtc aaaattccga actgcgcaca atcttgccgc aaaacgtcca gctccccttc
     4621 cagaagcgga gaccgattgg cctgtgccgt ctgcatcttg tccaatcgaa attggagatc
     4681 gttcacggca tctcgggctt gagaggcctg ggcctgggca gtctggagat cacgttggag
     4741 agcggccacc aaggcttgga gttccgtgcc ttctgtctgt cccacgcgcg cgtcggcctg
     4801 cgcttgattg agatcttctt cgagttgctc cgcccgagtc tcacgctcga ccagtgcccg
     4861 ttgtgtgtcc gccaatcgac gataatactc gacacgttcg tcctccaact gacgaatgtg
     4921 tcgggcaccg atccagcgcg tccagaccag cgtgctgcct gtggccaatg caagcgccat
     4981 gaaagggagg accaccggca gaacgagacc actcgaggca aacccagcaa acgacaccag
     5041 gagatagact gcgccggtca gagcgacagc gaccagtcca ccccagagtg agaaagccaa
     5101 caggcaccat gcagcccctg cgcctatccc catgaccaat acccagtgta gccagacggg
     5161 gacggtccat aaggcacttt ctgtcagaat ggtgttgaaa atattggcca aaatgaagcc
     5221 gcctggagcg gtgagttcaa gcggcgtctg tcgcttatcc gaccccagag cggcatgcac
     5281 gatcaaaata gccttccccg caacctgctc acggagttct gcatcacgcc cttcctgaat
     5341 tgcatcccac acatccacaa acgacaagta ggggaaaggg ccctctgtcc agcgccccgc
     5401 atagttgatc agaatgtcgc catgctcatc cacaggaatg gagagatttc tcttcgtacc
     5461 gtcggaaaat gtcgcttcct gaaaggtcaa agagcggcct ggagagagcg ccatccgatc
     5521 cggagtaatg tggagatacg cagccgccag cgcgaccccc agcgatggga tcgcctgccc
     5581 cccggcattc acaaacgacg ggactcgccg gtagacccca tccgcatccg aaatcgcacc
     5641 gatgtgtcct acgccgcgtg cagcttgcag cagacctgac aacggcccgg caatatgatc
     5701 cgtagtggga agcgcgtgga ggaccgccgg ttctaaggaa gggagggatc gcgccatgcc
     5761 gtttctgagt gggagcggca cagtcggtgc ttctccggca tggtcgctca aattcacagg
     5821 gagcgggaac aggacattgc cagcccgagt cgcagcctcc accaaggcgc gatcactgcc
     5881 tgctccgcca cgctccgcag ggctctcttc gccgaagtga aaatccagtc ccacgacctg
     5941 agcaccggcg cgatgcagcc cggagatcat ggtggcaaat accgcacgat cccagatccc
     6001 cacaccgaac cgagcatcac tctcggcatc acggccgatg aggagaaggc gcccgctgtc
     6061 gggtgtagcc cgatgccact gtgagcgaag gtcgaaggtc ttcgcttccc acgattcagt
     6121 cagaccgggg acaagcagca tcaacagggc aagaaggata gtgccagcga atccgatgac
     6181 aagactgaca ggcctcgaag ctttcatgtg ccgcacactc cgtcacggct ccctagaacg
     6241 tcagattgca gggcggagag tcgacacttc agcagaacca ttccgcaatc cttttccaaa
     6301 taggcggttc acctgactcc atctacttcc ttaccccctg aaacatcatc tcacctcagc
     6361 agtgccacgg gaaaattccg atagccgccg ggtgtgtcgg tgatcggatg ctgcgcgccg
     6421 ttggtgagat gcggcacccg ctccgataca tagccatcca gctccttcat cgatacgatg
     6481 ccatctttgg taatgagatc ggccttaccg ctcagtccct ccagtagcgc gtaggtgaag
     6541 gcgccgtgtt tccaggcatc tccttcttgg gagagctcat tgccggcact cgacgttaag
     6601 atcaccgcgt ccatcgcttg cagctctttg atgaaccgat tgttgtcgac cccgcgcgtc
     6661 ttcttcttgc tgacgccttc ggaatggcac gcgtccagga agacaatctt cttcgctggg
     6721 agatccagca ccttcttgat ctcgtcgttc gagatggcgc gggatcgctt gatcgacccg
     6781 tcagactgaa tcgccgcatc gctcggcaag aaatagaact ctccacgatc atcgttgatg
     6841 ccatgccccg cgaggaataa cagaactacg tcattctgcc cggcctgccg gagatacccg
     6901 aggttgtcca gaatcgtgtc gtaggtcggc ttcattgtgc tcgaatccga aatgatgaga
     6961 ctgcgcacat cccggaagag cttgcctcgc tgctgagtaa acgcctgaac gattgcctgg
     7021 gcgtcggctt cggcatagga cagagcgcga atctgtcgat cctgataggc attgattcca
     7081 atcgaaagaa tccagaggtt cggcttgatc acgtcgtcgt ggggtactag agaagcgggt
     7141 agcgaaacgg taatggcctt gactgtctcg ctatgaccat taaaggcctt cacttcaatc
     7201 acattctcgc cctcttccaa atccagcggg atggcggtcg tccattcgcg gacaccggca
     7261 ggaacggtcc ctggaacgac cggtgccttc tcaacgccgg atttggcggt cacgaccaag
     7321 tcacgagtct gaagcggcca tacctgagcg ccgttacgag aaacccgcac agactgaatc
     7381 gggtaactct tgtctgtaat cgtgaccgcc agatcaagtc tcggtgttgc cagaacagaa
     7441 cgatcttccg gcgaggagat cgccacggta ggcggagcaa taaaatcacc tgccccgcct
     7501 gtcgcccgct tatcaccttc acccaagatc tctgcaatgg cctgttcgcg attgccaagc
     7561 cgcaaagtcg cctcgacaat ctgggggcga tagaacgtgg agcgaaattg atcgatgcca
     7621 tacacctgat tgcccaaccg gacgtttaga tagttatctc ccttcgggga ggcagcgtaa
     7681 tagccctccg gggtcaggct aatccattcc ctgtcaccca gcagtaccgt agtgagtaac
     7741 tcttccccac tcgctacacg ccagacattc atccccatcc ccccagatac ggcaaggctg
     7801 ccatccggcg agagcgccac ggacctcaca gaatttccgt gccctatgaa gctccgcact
     7861 tcccggcccg tcgccacctc ccagagcttc aattccccac ccaagctccc cgagaggacc
     7921 cagcgcccat ccggcgaaaa cgccgcagac catacatcag aactgtgccc cgtgaaggtc
     7981 cgcacctccc ggcccgtcgc cacctcccag agtttcaccg tccgatcccc acttcctgaa
     8041 agggcccagc gtccatcagg ggaaaacgcc acggaccgta cgtcataact gtgcccagtg
     8101 aaagtccgca cctctcgacc tgtagccacc tcccagagcc atatcgctcc gcccacacta
     8161 cccatgagag cccaacgtcc gtctggcgag aacgctacgg aattcacagc tagccggtgc
     8221 ccggaaaagg cgcggatctc ccggcccgtc gccacctccc agagcttcat cgtctggtcc
     8281 ccactccctg aaagcgccca ctggccatcc ggcgagaatg ctacagacct caagtcagat
     8341 agcaggtgcc ccatgaagct tcgcacttcc cgccccgtcg ccacctccca gagcttcatt
     8401 gccccatcat cactccccga aagggcccag cgcccatccg gagaaaatgc cacagcggtc
     8461 accattacct tgtgccccgt gaacgtacgc acttctcggc ccgtcgcaac ttcccaaagc
     8521 gataccgccc catcgctgct ccccaagagt gcccagtgcc catccaggga caaggccatg
     8581 gagtacactg gcacccaata tcccttaaat ttcctcactt cctgacctgt agtcactgcc
     8641 caaagcttca accccgcgcc aacacttcca gagagggccc agcgcccatc cggcgagaac
     8701 gccacagact tcacatcgta ggtgcgctcc aacgtccgca cctcccggcc cgtcgccacc
     8761 tcccagagtt tcaccctccc gtcttcgccc cccgaaagcg cccagcgccc atccggcgaa
     8821 agagccacag agttcaccgc tgccgtgtgc cccatgaagc ttcgcacttc ccgccccgtc
     8881 gccacctccc agagcttcac cgtcccgctc ccgccagaaa gcacccact
//
LOCUS       DAORQN010000056         8648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_420_length_8648_cov_6.879204, whole genome shotgun sequence.
ACCESSION   DAORQN010000056 DAORQN010000000
VERSION     DAORQN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_8648_cov_6.879204"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK614_05015"
     CDS             complement(<1..221)
                     /locus_tag="PK614_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81604.1"
                     /translation="MAAEKQNQAISIEPASSAHRLILMLVAQRVAAPRTYFEHIGRLW
                     GKQKRKASRHFFGEFLNFGFRLAQFIFGQL"
     gene            301..1587
                     /locus_tag="PK614_05020"
     CDS             301..1587
                     /locus_tag="PK614_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRB81605.1"
                     /translation="MTIHVPIPMIALAEQYGNIHQSLAAHRHTQRDWVALGGGTGFLK
                     YRIWPGDQSSTTTGGRLLSHSSFPFGVEYAKQIKGTVTKIADCGQQDATTGTGRLSVT
                     LATTFSQARAYTLLPVSSVTAVEANRPCLLSEQGVDTAPLMAQVYRSELQGMLPTVDR
                     KAATLVTLKPAVTHIWNDLQEPLLLDEAEGLWLQLNPEAIGPAGVEPLSGTPAAGYGV
                     TARPAVVRGTKPLSRHLPLPDLQSQFHDDGFHVTFALQVPIEEANQRLREAVVGQEWS
                     LGVGTIKIVGATLYPLGNQVGVELALRGLLPLTLRLKGTPAYDEAAGRILFRDVDYTI
                     KERTPATDLAEEWLHEPLRDELAGRLVLPIREELDLMRQALEKGLNRNLTGGRLRGTV
                     HKLALEALTVQTANLSVRFTTEGTLHYDAHADIGAP"
     gene            complement(1792..2100)
                     /locus_tag="PK614_05025"
     CDS             complement(1792..2100)
                     /locus_tag="PK614_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HRB81606.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVSRSLDIIDKSGVDYRLNPMGTVL
                     EGEWDEVMAVVKKCYMRMRKDCNRVSCSIKIDYRKGPKGRLSSKVSSVESQLKRKLKV
                     "
     gene            complement(2150..2803)
                     /locus_tag="PK614_05030"
     CDS             complement(2150..2803)
                     /locus_tag="PK614_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRB81607.1"
                     /translation="MAANPGFQISLDVRGWPVLVIGGDEEAADKVQRLLEAGAKVTVI
                     SPTLHEVLRKLAASAKIIHRGRHFRANDLEAVILVLNTIRDDRELSHSLMRLAREQKF
                     LIWSVDQPDVSNVVMPAVVSSGHVRVAISSSGQAPALSGFMKEDLERILDNEFVAFVD
                     WLGQLREQAKANEPDAEKRRALLREALDGFRLLGKVQYPKVWLDQRAAVGAGEKRGS"
     gene            2978..3430
                     /locus_tag="PK614_05035"
     CDS             2978..3430
                     /locus_tag="PK614_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81608.1"
                     /translation="MAGYTGRSLAMAGGIIGIAAVAAYFGIAHIGEPDQGGGSIAGQV
                     QIAPNLADQVKSTDVLFVIVRRPTGPPRPIAAKRIDAPKFPVQFEISSADVMVQGTEL
                     KGMVDVIARLDRDGQAGQAQPGDMEGRYPKNPTLPGGKDVNITIDKVY"
     gene            complement(3559..4104)
                     /locus_tag="PK614_05040"
     CDS             complement(3559..4104)
                     /locus_tag="PK614_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRB81609.1"
                     /translation="MEMTLLLNSTYEPLRVLHWQKAITLLWQGKVEVLEVYDRQIHGI
                     SISIKLPSVMRLLKLVKLKDSHRAVKFSRINIFTRDGYCCQYCKHKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPEEANMRLIKRPVKPRWSPTVTITIGIRNT
                     PESWRDYLYWNLELDADPAET"
     gene            4303..4629
                     /locus_tag="PK614_05045"
     CDS             4303..4629
                     /locus_tag="PK614_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRB81610.1"
                     /translation="MDSKPDYITVAQVDQVPPGTCRTVEVEGIFLALCNVDGTFHVVD
                     NTCPHAGGPLGEGCMDGELVECPWHGWRFNVRTGERPENPEIRVACVEVRVQGTNVQV
                     KTPLTL"
     gene            complement(4619..5074)
                     /locus_tag="PK614_05050"
     CDS             complement(4619..5074)
                     /locus_tag="PK614_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81611.1"
                     /translation="MSKVIHYTVLALLLAGGLAYWWMKPPGLNPMLDSQAAEALALVQ
                     THRAAGYPTILQAFNERARIQEARKLGLRLGEWKVIKQDGQRYEVRIYMREQGTTQWF
                     ERDFIWHVDLATKRVNAASLPADGLMPEGPESGRPRAPGGEVPPFPPTM"
     gene            5276..5719
                     /locus_tag="PK614_05055"
     CDS             5276..5719
                     /locus_tag="PK614_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRB81612.1"
                     /translation="MKFFFYADNLNLSQLKRRAPEHKFLFTAYLPDHSIQFCRWSTQW
                     RCGLASVIPSPGEKVWGAVFEITDEDLKILDEFEGDVPQGAYRHLPVTVITEEGEKLL
                     VTTHAANPIGKFKPKEHYIDWVLKGIKQWKLPEECTEQWKAYKPA"
     gene            5763..6089
                     /locus_tag="PK614_05060"
     CDS             5763..6089
                     /locus_tag="PK614_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRB81613.1"
                     /translation="MPKPKYHILVCTNARPPGHPKPSCGGQGSAQLLMSFNMGLMQRG
                     IMPGEVLVTGSSCLGPCEQGPTVVVYPDNTWYSKVTEADVATILDEHIKGGKPAAKLN
                     PDSVWK"
     gene            6183..6695
                     /locus_tag="PK614_05065"
     CDS             6183..6695
                     /locus_tag="PK614_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRB81614.1"
                     /translation="MSSPTAHEHKHHAPRSIGCMVITCSDTRTPETDTSGQLIQQLLK
                     DKGHHIAAYHLVKDEPAEIKARIAEAITNESVQAIVINGGTGISRRDSTFEAVDAMLE
                     KRLDGFGEVFRYLTYQDIGSPAIMSRATAGIIKGRILFSTPGSENAVRLAMEKLILPE
                     LGHLVKELTK"
     gene            complement(7042..7476)
                     /locus_tag="PK614_05070"
     CDS             complement(7042..7476)
                     /locus_tag="PK614_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB81615.1"
                     /translation="MASIAQIMNKKPQSIGPTTSIRGAAKKMRDLRVGSLLIKKGKNF
                     VGIVTDTDLVRKGLATSQDVSKLTVDKIMTTPLCTIESNQAVDDAQDMMGDLGVRHLA
                     VTKGGSIVGVVSVRDLLMHYKRYAQSRLADKQVYSEPKIGQD"
     gene            complement(7527..8558)
                     /locus_tag="PK614_05075"
     CDS             complement(7527..8558)
                     /locus_tag="PK614_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRB81616.1"
                     /translation="MNAVVFHEHGGPGKLQYQEMPMPTIGVDEVLVRVKACALNHLDI
                     WIRQGSPAYPMPLPHILGSDISGVVHQIGSHVEGVAEGERVYVSPGVGCGRCEHCWAG
                     RDNMCRSYGLIGAMCHGGYAEYVKVPARNVFPVPGALSFEQAAAFPLVSVTAWHMLFG
                     LAAVQPGEDVLIMGAGSGVGHMAIQMAKLAGARVLTTVGSDDKIAKAKALGADEVINH
                     TREQVNQRVRELTHRRGVDVVIEHIGPEVWTQCIDALAKGGRLITCGATTGAEVKLDL
                     RYIYSRQLTIRGSYMGSQSELLKAAQLVGQGRLQPVIDRTFPLAEAKAAQEYLLNRKF
                     FGKIVLSVS"
BASE COUNT         1860 a         2680 c         2405 g         1703 t
ORIGIN      
        1 agctgaccga agatgaactg cgcaagtcgg aagccgaaat tcaaaaactc accgaagaag
       61 tgacggcttg ccttccgttt ttgcttcccc catagtcgac cgatatgttc aaagtaagtt
      121 cggggggcgg ccaccctctg tgccaccagc atgaggataa gacgatgggc ggacgacgcc
      181 ggttcgatcg aaatagcctg gttttgcttc tctgcagcca ttctgagtct ctgcgccaca
      241 gcacccgcgg cagcggcagg agccgacaca gcccggcacc agcagacgga agcctccagc
      301 atgacgattc atgtgccgat tcccatgatc gccctggccg agcagtatgg gaacattcac
      361 cagtcgctcg ccgcacaccg acacacccag cgcgactggg tggcgctggg cggaggcacc
      421 ggatttctga agtaccggat ctggccggga gaccagagtt cgacgacgac cggaggtcgc
      481 ctgttgtccc actctagctt tcccttcggc gtggaatacg cgaagcagat caaaggcacg
      541 gtgacgaaaa tcgccgactg cggccaacag gatgccacaa ccggcacagg ccggttatcg
      601 gtgacgctgg caaccacctt cagccaggcc cgcgcctata ccctattgcc cgtcagtagc
      661 gttaccgcgg tggaagcgaa ccgaccctgc ctgctgtccg aacaaggcgt cgatacggca
      721 cccctgatgg cgcaggtcta tcgaagtgag ttgcaaggga tgctgccgac ggttgatcgt
      781 aaggctgcca ccctcgtgac gctgaagccc gccgtcactc acatctggaa cgatctgcag
      841 gaacccttgc tgttggatga agcggaaggc ctctggctcc aactgaatcc tgaagccatc
      901 ggccccgccg gagtcgaacc gctctcagga acaccggccg cggggtatgg cgtcacagcc
      961 agaccggccg tcgtgcgcgg taccaagccc ttatcacgac atctccccct ccccgacctg
     1021 cagtctcagt ttcacgacga cggctttcac gtcaccttcg cgctccaggt ccccatcgag
     1081 gaggccaatc aacggctgcg tgaagcggtg gtcgggcagg aatggtcgct gggcgtcggc
     1141 acgatcaaga tcgtgggagc gacattgtac cccttgggca atcaagtggg agtggaactc
     1201 gccctgcgag ggctgctccc gctcacactc cgtttaaagg gaacaccggc gtacgacgaa
     1261 gcggcggggc ggattctgtt tcgggatgtc gactacacga tcaaggagcg cacgccggcc
     1321 accgacctgg ctgaagaatg gttacatgaa ccgctgcgtg atgagttggc cggaagattg
     1381 gtgctcccca ttcgagagga actggacctg atgcgacagg cgctggagaa gggactgaac
     1441 agaaacctga cgggagggcg cttgcgcggc acggttcaca agctggccct cgaagcgctg
     1501 acggtgcaga ccgccaacct ctcggtgcga ttcacaaccg agggaacact tcactacgac
     1561 gcccatgcgg acatcggagc cccgtaaccc gcaggatgtg caaaaagtcc gtcagcggcg
     1621 ttctcgggtc gcttcgcggc tcaccgtacc gcacgagtac gattcgcctc ttcgctcact
     1681 gcggccttgc tggacggcct ttttgcacat cctgcgggtt atggtgatac cgaaacggca
     1741 cataagttga ttgtagcgta ctgcgcaaca atcgagtttt tccgcagcct gttagacctt
     1801 cagctttcgc ttcagctgtg attccacact cgacactttg ctcgacaagc gccccttcgg
     1861 tcccttgcgg tagtcgatct tgatgctgca ggacacgcga ttgcagtctt tgcgcatgcg
     1921 catgtagcat ttcttgacca ccgccatgac ctcgtcccat tccccttcga gtaccgtgcc
     1981 catcggattc agccgatagt ccacaccgct cttgtcgata atatccaacg agcgtgacac
     2041 gtatttgctc acgctctctc ccttgcccag gggcgacata ctgaattcca gcaacaccat
     2101 agtgggaatc tcctgctgat aactgtcggc cgataacgaa cgaccggtcc tacgatccgc
     2161 gcttttcccc tgccccaacg gccgcccgtt gatccagcca caccttcggg tactgcactt
     2221 ttcccagaag tcggaatcca tccagcgcct cacgcaacag cgcgcgacgt ttctccgcgt
     2281 ccggttcgtt ggccttggcc tgctcgcgaa gctgcccgag ccaatcgacg aacgcgacaa
     2341 actcgttgtc caaaatacgt tcgagatctt ccttcatgaa tccggaaagc gccggcgcct
     2401 gcccgctcga gctgatcgcc acacgaacat gaccggagct gacgacggcc ggcatcacca
     2461 cgttcgacac gtcgggctgg tcgaccgacc agatcaaaaa tttctgttcg cgagccaatc
     2521 gcatgagcga gtgggagagt tcacgatcat cgcgaatcgt gttcaagacc aggatgaccg
     2581 cctccagatc gttcgcgcgg aagtgccgcc cacgatgaat gattttcgcc gaggccgcca
     2641 gtttacgaag cacttcatgc agggtgggac taatgaccgt gaccttcgcg cctgcctcta
     2701 ggagccgctg cactttatct gccgcttcct catcaccacc gatcaccagg accggccacc
     2761 cacgcacatc gagtgaaatc tgaaacccgg gatttgcagc cattgagttc atcctccaac
     2821 aaatcgacaa cgacgtgaac gcgtatcata caacagccga tttgacaggg taaacccgct
     2881 ccgccattcc cctttctggc aggcgctggg tataatgcgc ccgctggatg acgggcgagc
     2941 ccgtcgcgcg caccgcgagt caacaaagga gtctattgtg gctggatata caggacgatc
     3001 cctggcgatg gccggaggga tcatcggcat tgcggccgtc gcggcctatt tcggcatcgc
     3061 ccacatcggg gagccggatc aaggcggcgg atcgattgcc ggacaagtgc agatcgcgcc
     3121 caacctggcc gaccaggtca aatcgaccga tgtgctgttc gtcatcgtgc ggcgtccgac
     3181 cggaccaccg agacccatcg cggccaaacg gatcgacgcg ccgaaatttc cggtgcagtt
     3241 tgagatttca agtgccgatg tcatggtcca gggcaccgaa ttgaaaggca tggtggatgt
     3301 gattgcgcgt ctggatcgag atggccaggc cggccaggcg caaccgggtg acatggaggg
     3361 acgctacccg aagaatccga cgttaccggg cgggaaagac gtgaacatta ccatcgacaa
     3421 ggtgtattag ctgcggcagg ctgtctgaac aggtgattcg gtaaagctgc aacaagcgag
     3481 aggccgggcg cacttggctt gtaccttgaa gtggtgagca ccgcgagtgc gatgccaaga
     3541 acccgtttca acagccggct aggtttcagc cgggtccgcg tccagctcga ggttccagta
     3601 cagatagtcc cgccaacttt ccggcgtgtt ccggatcccg atggtgatcg tgacggtagg
     3661 actccagcgc ggctttacgg gcctcttgat caaacgcatg ttggcttctt ccggggtccg
     3721 tccgctcttc cggttattgc accgccagca ggccgtcacg atgttctccc aggtcttccg
     3781 accgcctttc gcgatcggca cgacatggtc gaacgtcagt tcctcggtgc ggaacttgtg
     3841 cttgcagtac tggcagcagt agccgtcgcg cgtaaaaatg ttgatgcggg aaaacttcac
     3901 cgcgcgatgg ctatccttga gcttcaccag cttcaggagg cgcatcaccg aggggagctt
     3961 gatcgagatc gagataccgt ggatttggcg atcgtagact tcgaggactt cgaccttccc
     4021 ctgccagagc agagtaatgg ccttttgcca atgcaggaca cggagcggtt cataggtgga
     4081 gttgagcaac aaggtcattt ccatagtggc tccggcgatc tcctatgagt gcactattta
     4141 cagaatagca aatgacggcg ctgttgtgac ccttccctga aaaatgcctg ctcaatctat
     4201 gccatatgcg gccaatgaaa tcaagcgctt aggcaatacc gcacacaatg tcacgcaata
     4261 ttgagacgac ctgtaccccg ctgtaacttg cagaggacgc ttatggattc gaagcccgac
     4321 tatatcaccg tcgcccaggt ggaccaggtt cctcctggca cctgccgcac cgttgaagtc
     4381 gagggaattt ttctcgccct ctgtaatgtc gacggaacct ttcatgtcgt cgataataca
     4441 tgcccgcacg cagggggacc actcggcgaa ggatgcatgg atggtgaact ggtcgaatgc
     4501 ccctggcacg ggtggcgatt caacgtccgg accggtgagc ggcctgaaaa tccggagatc
     4561 cgcgtggctt gcgtggaagt gagggtgcaa ggcacaaacg tgcaggtgaa aacgcccctt
     4621 acattgtagg cgggaacgga gggacttcac cgcccggtgc gcgcggtcgt ccactctccg
     4681 ggccttccgg catcaacccg tcggccggca acgaggccgc gttgacgcgc ttcgtcgcca
     4741 gatccacgtg ccagatgaaa tcccgttcga accactgggt cgtcccctgc tcgcgcatgt
     4801 agatccggac ctcatagcgc tgaccgtcct gcttgatcac tttccactct cccaagcgca
     4861 agcccaactt gcgggcttcc tgaatgcggg cacgctcgtt gaacgcctga agaatcgtcg
     4921 gataaccggc ggcccgatgg gtttgcacca gcgcgagggc ctcggccgcc tgcgagtcga
     4981 gcatcgggtt gaggccgggt ggtttcatcc accaataggc caaccctccg gccaggagaa
     5041 gcgcaagcac tgtgtaatgg atcactttac tcataggtcg tcacaataac ctgcggtgga
     5101 tggttgatca actcgtcatc ataggtggcg tgtgaattca ctgtcaatcg tcgaacaaca
     5161 gcgggaggcg ttgaaaccgg cgacgcgcag gagcccctgc gcttgacaag gtcgcgcaac
     5221 gctatggtac aagtaccggt caaccttcgc gacataatcg gagtctcact gttcaatgaa
     5281 gttcttcttt tacgcggaca atttgaacct gtctcaactc aagcgtcgcg ccccggaaca
     5341 caagtttctt ttcacggcgt accttcccga ccatagcatt cagttctgcc gctggtcgac
     5401 gcaatggcga tgcggcctgg cgagcgtgat tccgtcgcct ggggaaaaag tctggggagc
     5461 cgtcttcgaa atcaccgacg aagatctcaa gatcctcgac gaattcgaag gtgatgtacc
     5521 gcagggcgcc taccgccacc tgccggtaac cgtgatcacg gaagaaggcg aaaagttatt
     5581 ggtcactacc catgcggcca atcccatcgg caagttcaag ccgaaagagc attacatcga
     5641 ttgggtactc aagggcatca agcagtggaa gttgcctgag gaatgcaccg agcagtggaa
     5701 agcgtataaa ccagcctgac gaactcacgc agtcgtcgct caccccatac attgaggtca
     5761 cgatgccaaa accgaaatat catattcttg tctgcacgaa cgcccgtccg cccggccacc
     5821 cgaaaccctc ctgcggcggc cagggctctg cgcaactgct gatgtccttc aacatgggtc
     5881 tcatgcagcg cggcatcatg cctggcgaag tcctcgtcac cggctcctcc tgcctcggcc
     5941 cctgcgagca gggaccaacc gtcgtggtgt acccggacaa cacctggtat tcgaaagtca
     6001 ctgaagccga tgtcgctacg attctcgatg agcacatcaa gggcggcaaa ccggcggcga
     6061 agctgaaccc ggattctgtc tggaagtagc tcgcgaacgc cagagcggag atggatcaaa
     6121 aaggccgccc agcgaagcga gttcgatgct ggcggccttt ttaaccttta gcggatatcg
     6181 atgtgagtag ccccacagcc cacgaacata aacatcacgc accccgctcc atcggatgca
     6241 tggtcatcac ctgcagcgat acccgcacgc ctgagaccga caccagcggc caattgatcc
     6301 agcagttgct taaggataag ggccaccaca tcgccgccta ccacctcgtc aaagacgaac
     6361 cggcagagat caaggcgcgg atcgccgaag ccatcaccaa tgagtccgtg caggccatcg
     6421 tgatcaatgg cggaacaggg atctccaggc gggactcgac gtttgaagcg gtggatgcga
     6481 tgctggaaaa acggctggat ggattcggcg aagtgtttcg gtatctgacc tatcaagaca
     6541 tcggctcacc ggctatcatg agccgggcca ccgccggcat catcaagggc cgtatcctct
     6601 tttcgactcc cggatcagag aacgccgttc gcctggcgat ggaaaaactg atcctccccg
     6661 aactcggcca cctcgtcaaa gaactcacca agtaagaacg acacgtgaga ggcgaaaggt
     6721 gaatcgatca cctcttcacc tctcacgttt gacgttttac tcttaacgcc ctatccccgg
     6781 cagtcacgga tgctccaaac ggtcatccaa cgaggccaca gggagtgcgg cgactgaggc
     6841 gtaccctcag gggtacgttg aggattttga tgagccgaga acgaagctgg agggtgtttt
     6901 cagcatccgc cctgtgaagg atgttcaaaa cggccgtcca gcaaggccgc aggaagcagg
     6961 gcgactgagg cgtacccttg cggtacgtcg cagggagccg tgcgcccgag aacgctgctg
     7021 gaggccgttt tcaacatccg cctagtcttg cccgattttc ggttcggaat acacctgttt
     7081 gtcggcaagt ctggattgcg cataccgctt gtagtgcatc aagagatcgc gcaccgacac
     7141 cacgccaacg atcgaaccgc ccttggtcac tgcaagatgg cgcacaccga gatcgcccat
     7201 catatcctgc gcgtcatcga ccgcctgatt gctctcgatg gtgcagagcg gggtggtcat
     7261 gatcttgtcc accgtgagtt tcgatacgtc ctggctggtc gccagtccct tgcggacgag
     7321 atccgtgtcg gtcacgatcc ccacaaagtt cttccccttc ttgatcagga gtgaaccgac
     7381 ccgcaagtca cgcatcttct tcgccgcacc gcgaatcgac gtcgtcggac caatgctctg
     7441 gggtttcttg ttcatgatct gcgcaatact cgccatgtca catacctttc tgcccgttgg
     7501 gacaagagag ccactaatga aaccggttat gaaacgctca gcacgatctt accaaaaaat
     7561 tttcgattca gaagatattc ttgcgccgcc ttcgcttcgg cgagtggaaa cgtccggtcg
     7621 attacaggct gcaacctgcc ttggccgacc aactgagcag ccttgagcaa ttcactctgc
     7681 gaccccatgt acgaaccacg gatcgtgagc tgccgggaat agatataccg gagatccagc
     7741 tttacctccg cgcccgtcgt ggcgccgcag gtaatcaacc ggccgccttt ggccaatgca
     7801 tcgatacact gtgtccacac ttccgggccg atgtgttcga tcactacatc cacgccgcgg
     7861 cggtgcgtca actcgcgcac ccgctggttc acctgctcgc gggtgtgatt gatgacctcg
     7921 tcggcgccga gggccttggc cttcgcaatc ttgtcatcgc tcccgacggt ggtcaacaca
     7981 cgggcaccgg ccagcttggc catctggatt gccatgtgcc ccaccccgct gcccgcgccc
     8041 atgatcaaca cgtcttcgcc gggctgcacc gcagccaacc cgaacaacat atgccaagca
     8101 gtgacagaca ccagcggaaa cgccgccgcc tgctcgaaac tcaacgcgcc gggaaccggg
     8161 aacacattcc gcgccggcac cttcacgtac tcggcatatc cgccatggca catcgccccg
     8221 atcagcccgt aggaccggca catgttgtcg cgaccggccc aacagtgctc gcaacgaccg
     8281 cagccgactc cgggagacac atacacgcgc tcgccctcgg caaccccttc cacgtgcgaa
     8341 ccgatctggt gcaccacgcc ggagatgtcg gacccgagga tatgcggaag cggcatcgga
     8401 taggcgggac tcccctgccg gatccagatg tcgagatggt tcaacgcgca ggctttgaca
     8461 cgcacgagca cttcatcgac gccgatggtc ggcatcggca tctcttgata ctgcagcttc
     8521 cccggcccgc catgttcgtg gaacaccact gcgttcatct gattcctctc acacgatttc
     8581 tggctacatt gcgctcccct ctcaaacgtc ttttcctggg gtgcaactgc ctatgatttc
     8641 ccacagga
//
LOCUS       DAORQN010000057         8583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_421_length_8583_cov_17.394114, whole genome shotgun sequence.
ACCESSION   DAORQN010000057 DAORQN010000000
VERSION     DAORQN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_8583_cov_17.394114"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="PK614_05080"
     CDS             complement(<1..1886)
                     /locus_tag="PK614_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81617.1"
                     /translation="MANNALDDILNMEERTDQTSMPEDTTSLDDIINFEDRKGGETST
                     DVPPNPHGVMHTAAEAAGGAWASAGSLAGNAGFAAPGGPITGVAGRMAGAGLGGALGY
                     GGYRAHEDLRDGGVPQMLSNLGDTLVESAKFGATSALTEGIVPGAGWLYRSTPIRAAR
                     EWVSKKWGESLSGQLDRVIDSVGTTMRDVDNLVRTGQRRAALKAGTDRPAAGLTLGQL
                     SDDPSSTANALEGVAQSTWFGNTASRQRQWSSEGANQFADDLAKQLQGASTEEVGQGL
                     TWAVKDNFKNLYSAAADTAYDGLRAATAGTRPVVAVPILKDLRTPNSTLGNNVITELK
                     KLRSVNPDPQAIAEIDNLINTLSVTQSQANKGALTRIPPNLSLDQALRLKTILGGIAD
                     SKAGLDSMADTAAKTTAETYAKQIDDAIRKGLGKSPDLLREYDSAAAHYAQGVATFKN
                     ETVKKVVDGLTNSPGALESILLKPEQRETIAAVKQAVGPMWDSQVAPKLRSIMLMNAK
                     DGDTFSGQKLMSSIIDLIGPNPAKNNTMVEIFGEKATKELIGVAHALELTHNPAKGAW
                     WVKLKQASAIGVIGSAGLGVATGHQAGGVGMGATILITPWALGHLTANPNMLKAFKDG
                     IVATS"
     gene            complement(1876..4671)
                     /locus_tag="PK614_05085"
     CDS             complement(1876..4671)
                     /locus_tag="PK614_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81618.1"
                     /translation="MDIKPFLAGMPLGPISGVASILMGAEMSQRRREQEQAAKDLEER
                     RKVEMKEVEGYASKGNHAMLQQAYDSGMYTALPQDYRDRSLFRAGTYSNQQIALDADT
                     ASKAGFQPYMGTPQQMQQAMAMTGQPGAPQGMTPQPPPMMAQQVQAALPPGAPGQAAS
                     SMEMMPQIGGAPPPPQALPPTATSQGGQVMTPESRQFTPIQAAQEPQFDYQGRPMPPQ
                     PQADLIPQPIREEPIVMGQRKQFGPGTQPLALKFPVGIRPGKASVEGPLGTVPGKGEE
                     LQNSTAISQTMVDQAKGMGMSPDQLDAEYAKQIAKSGGNYPAFDETIRKTYKQEYFQK
                     LAQDKTRALVAAGLPEPQAQQQGARLAADTMGGYYPEGAGQLVNYTPEERHRNVIGQA
                     TKQVMIQARAAMDASRPGEPATIQPGLFRKYVEEAFGTDGTPQEKEAALNDVTNMAMT
                     TFRDKIGKLPGMTQAQAMIMSMQAAAGLAGYTPKEWQPMIMANAQAFGITDPDIAQMV
                     ATGEIDLLQPGSLQAGITKKNALAYEGKIREKGMERQFAVTQTPEGVAATQQALTTQP
                     SAPPAASPGAAPVGAGSPAAPSQTPGEIDRAIKTQQVAAETEAKEKVQKQYRLAPEKE
                     IQDVRALDDMYTKGTRLLQIGEGTPDKPGLIDRYGNYPGTFFDWTQSIKSATGTMDPD
                     LKEYVNTLSGLFNIEKKEFTGTATSLNERKDLVAWIPDKSTNVRDALQSVSQLVKSVS
                     SDLKRNTEHIASNYPGTDLSSLKYNQIQQRRLARQQQQGTQPPLATAPPPPPTPPTPA
                     PEPSAPAPAPAQSSSLPSISEQADRTKAAHPSKATPKFGPGSTKAAAALVPNEIQQSL
                     TPQDKGELHTAMKQYSRGQIDDKQLELAVAKVLMRAGTHSGKKNLEGPALNKAVAAWT
                     EVLRRGAKSKKEFARGQ"
     gene            complement(4671..5210)
                     /locus_tag="PK614_05090"
     CDS             complement(4671..5210)
                     /locus_tag="PK614_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81619.1"
                     /translation="MGGNKAQQPSASKEQENRSQSLFDTWNPLQQSLAGQGNQLVQTG
                     GISQMIPMLTTLLNSARSGFSKSMNSLNDSLGRSRLAGTPFGQRLKSDQTMAGEQQVA
                     STIPNFYQWFLPQVMNAMTGNTATGMQGMSAVTGAETSRVNANAANQTQLQNTMMNNG
                     TQMMMKMMPTTSYSYTGTI"
     gene            complement(5688..7391)
                     /locus_tag="PK614_05095"
     CDS             complement(5688..7391)
                     /locus_tag="PK614_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81620.1"
                     /translation="MRAEWHDFSKGLWSVGGKEHTMTGYIRRAKGLHAIRTPHLRSRD
                     GSTKLYDLTAHSLFRFDDVQIQAAGGSLYRAGAVVTSSLNGNRLRFVPMPAQPGLNDY
                     LFITGGGLALKLDDGGSVTTWGITNPGGPPSLAETSAGTGALANGVYKYKVVFRNGST
                     GSRSNAQDIDASITIASGPSSVALTNIPVSSDGQVTEREIYRTTVGGALYFLAITISD
                     NSTTATTDNVADADLLSFQLQTDNDRPYDSCAEVWFHDSVMWMTRDTQPGTAGRAYYS
                     PIGRPESLLGFVEVSNTDDPCQVGFSFAESNWVMTQKNIYRITGDAEPFIPRKVYQCP
                     GTIYPYTVVVTPFGVVYQSFDGIRLFDGNNSTLIGFEAIGPIFRGQTIENLGPFVGTT
                     ATYAKEEYIISDGTTTLAINLRLGTWRDLGIGCSALYTEQDTPNVIASFNFGVYALED
                     YSTVLDGTDAIDLEWELGAKISDISQRTVVQRVYIDIDTGGQLLTPTLVIDNTTVELP
                     TFLTNSRELVEYSINRTCRLVGLRLTGQIEDVVNLYAVELDLAMGVSNSTNQQASQGR
                     G"
     gene            complement(7395..7844)
                     /locus_tag="PK614_05100"
     CDS             complement(7395..7844)
                     /locus_tag="PK614_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81621.1"
                     /translation="MASRLAWRAPKEYSSSAFDTLILAINQRLDELSVSARAADPTSS
                     TTLPVIADVVNNLVIGRGHYFELTITTANAGLTGLTGGEPGRIIILKNVSGSSNSLPL
                     IHNSSASTLGNRFINRAGTNVTVPVGGARVLLYSGELQGWVPIAEVP"
     gene            complement(7844..8428)
                     /locus_tag="PK614_05105"
     CDS             complement(7844..8428)
                     /locus_tag="PK614_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81622.1"
                     /translation="MSKSSILDLVQTFALGDADPLTAEQYYDHIIDDTGRAPWFVTAS
                     LVTMVAGTSTYALADDQLKILGMFYDDRWLDRMDHRALESLNPAWRDEQGLPVAYSVE
                     DEATKTFRLYPTPNRASGNFIFMFGSPLGLDYPPYAVLLLHTQLTPDVLEFMELPVMW
                     EVLSREFGHESNHRDDDLADFAQQFADVLWGMIS"
BASE COUNT         1466 a         2194 c         2931 g         1992 t
ORIGIN      
        1 gaggtagcga cgatcccatc cttgaacgct ttgagcatgt tggggttggc ggtcaggtgg
       61 ccgagagccc agggggtgat caaaatggtg gcgcccatgc ctactcctcc tgcttgatgt
      121 ccggtagcga caccaagtcc agcactgcca atgacaccaa tcgccgaggc ttgtttgagc
      181 ttcacccacc acgcaccctt ggcggggttg tgggtgagtt cgagggcgtg ggcgaccccg
      241 atcagctcct tggtggcttt ctcgccaaat atttcgacca tggtgttgtt cttggcggga
      301 ttaggcccta ttaaatcaat gattgaggac ataagttttt gcccgctgaa cgtgtcgccg
      361 tccttggcgt tcatgagcat gatggagcgg agcttaggtg cgacttgtga gtcccacatc
      421 gggccgaccg cttgcttgac tgcggcgatc gtctcgcgtt gttcaggttt gaggaggatc
      481 gattcgaggg cgccggggga gttggtgagc ccatcgacta cctttttgac cgtctcgttt
      541 ttgaaggtgg cgaccccctg cgcgtagtgg gcggcggcgg agtcgtactc gcgcaacaag
      601 tctggtgatt tgcccaatcc ctttctgatc gcgtcgtcga tctgcttggc gtaggtctcg
      661 gcggtggttt tggcggcggt gtcagccatg gagtcgaggc cggctttcga gtcggcaatc
      721 ccacccagga tggttttgag ccggagggct tgatccaggg ataggtttgg ggggatgcgg
      781 gtgagcgcgc ctttgttggc ttgggattgc gtcaccgaga gggtgttgat gaggttgtcg
      841 atttcggcga tggcttgggg gtcagggttg acgctacgga gttttttcag ttcggtgatc
      901 acattgttgc cgagggtcga gttgggggta cgcaaatctt taaggatagg cactgcgacc
      961 actgggcggg tgccggcagt ggcagcgcgg agaccatcat aggcggtgtc ggccgcagcg
     1021 gagtagaggt ttttgaagtt gtctttgacc gcccaggtaa gaccttggcc cacttcttct
     1081 gtggaggcgc cttggagctg cttggcgagg tcgtcggcga attgattggc gccctcgctg
     1141 gaccactggc gttggcggct ggcggtgtta ccgaaccatg tggattgggc cacgccttcg
     1201 agcgcgttgg cggtggagga tgggtcgtcg ctgagttggc cgagggtaag cccagcggcg
     1261 gggcggtcgg tgccagcctt caacgccgct cgacgctgac cagtgcggac gaggttatcc
     1321 acatctcgca tcgtcgtacc tacactatca atcacacgat caagctgccc acttaaactc
     1381 tcgccccatt ttttactcac ccactcgcgg gcggcgcgga tgggggtgga gcggtagagc
     1441 caccccgcac cagggacgat cccttcagtg agtgccgagg tggcgccaaa ttttgctgac
     1501 tcgactaggg tgtcgccgag gttggagagc atttggggga ctccaccatc gcgcaggtcc
     1561 tcatgggcac ggtagccacc atagccaagt gcgccgccga gtcctgcccc tgccatgcgg
     1621 ccggccacgc cggtgatcgg tcccccaggg gccgcaaacc ctgcgttgcc ggcgagggat
     1681 ccggcgctcg cccatgcccc accagccgct tctgctgcgg tgtgcatgac gccgtgaggg
     1741 ttggggggca cgtcagtaga tgtctccccg cccttgcgat cctcgaaatt gatgatgtcg
     1801 tcgagcgagg tggtgtcctc gggcatggag gtttggtcgg tgcgctcctc catgttgagg
     1861 atatcatcaa gggcattatt ggccacgcgc aaactccttt ttcgatttag cccctcggcg
     1921 gagtacttca gtccaggctg cgacggcttt attgagggca gggccttcaa ggttcttttt
     1981 accactgtgg gtaccagcac gcataaggac cttggccacc gcgagttcga gttgcttgtc
     2041 gtcaatctgg ccgcgagagt attgtttcat tgcggtgtgg agctcgccct tgtcctgcgg
     2101 ggtgagggat tgttggatct cgttggggac gagggcagcg gcggctttgg tcgagccggg
     2161 gccgaatttg ggggtggctt tggaggggtg cgcagctttg gtgcggtcgg cctgctcact
     2221 gatagatggc aaggaggagg attgtgcggg cgcgggcgca ggggccgacg gttcgggtgc
     2281 gggtgtcggt ggtgtcggtg gcggcggtgg ggccgtcgcg agcggaggtt gggtgccctg
     2341 ttgctgttga cgagcgaggc ggcgctgttg gatctggttg tatttcagac ttgagagatc
     2401 ggtgccggga tagttcgagg caatgtgctc ggtattgcgt ttgaggtcac tcgacaccga
     2461 tttgaccagt tggctcactg actggagcgc atcacggacg ttggtggact tgtcagggat
     2521 ccacgccacg aggtctttgc gctcgttcaa gctggtggcg gtaccggtga attctttttt
     2581 ctcgatgttg aataatccac tcagggtatt gacgtactct ttgaggtcag gatccatcgt
     2641 gccggtggcc gatttgatcg actgcgtcca gtcgaagaac gtgccggggt aattgccgta
     2701 gcgatcgatg aggccaggtt tgtcgggggt gccttccccg atttggagga gacgagtccc
     2761 cttcgtatac atgtcatcca gcgcccgcac gtcctgaatt tccttctcgg gagccaatcg
     2821 gtattgcttc tgtacttttt ccttcgcctc tgtttcggcc gccacttgct gggttttgat
     2881 cgcacgatcg atctcgccag gagtttgcga gggcgcggcg ggactgccag ccccaacggg
     2941 agcggcgcca gggctggcag cgggcggggc agaaggttga gtggtgaggg cttgctgagt
     3001 ggcggctaca ccttcgggag tctgggtgac agcgaactgg cgctccatgc ctttttcgcg
     3061 aattttgccc tcgtaagcga gtgcgttctt tttggtgatg cccgcttgga ggctgccggg
     3121 ttggaggagg tcgatttcgc cggtagccac catttgggca atgtcggggt ccgtgattcc
     3181 gaacgcctgg gcgttggcca tgatcatcgg ctgccattct ttaggggtgt agccggcgag
     3241 cccagccgcc gcctgcatgg acatgatcat tgcttgggct tgagtcatgc cggggagctt
     3301 gccgatcttg tcgcggaagg tcgtcattgc catgttggtg acgtcgttga gggccgcctc
     3361 tttttcctgg ggggtgccgt cggtgccaaa cgcctcctcg acatacttac gaaataaccc
     3421 tggttggatc gtggcaggct caccagggcg cgaggcatcc atcgcggcgc gggcttggat
     3481 catcacttgc ttcgtcgctt gtccaatcac gttgcggtgg cgttcctctg gggtgtagtt
     3541 gacgagttgg ccggcgcctt cggggtagta accgcccatc gtgtcggcgg cgaggcgtgc
     3601 cccttgctgt tgagcttgtg gttcggggag gccggctgcg acgagcgcgc gggttttgtc
     3661 ctgggccaac ttctgaaaat actcttgctt gtaggtcttg cggatggtct cgtcgaacgc
     3721 ggggtagttg ccgccggatt tggcgatctg tttggcatac tcggcgtcga gttggtcagg
     3781 ggacatgccc atgccttttg cttggtcgac catggtttgg gagatggcgg tcgagttttg
     3841 gagttcttca cccttgccgg ggacggtgcc gaggggaccc tcgacactcg ctttgccggg
     3901 tcgaatgcca accgggaatt tgagggcaag aggttgcgtg ccggggccga attgcttgcg
     3961 ctggcccatc acgattggtt cttcgcgaat gggttggggg atgaggtcgg cctgcggttg
     4021 tgggggcatc gggcggcctt gatagtcgaa ttggggctct tgggcagctt ggattggggt
     4081 aaattgtcgt gattcggggg tcatcacttg cccaccttga gaggtcgcag tgggagggag
     4141 tgcttgcggc ggcggagggg cgccaccaat ttgcggcatc atttccatcg aggaagccgc
     4201 ctgaccagga gcgcctggcg ggagcgcggc ctggacttgc tgggccatca tcggcggcgg
     4261 ttgaggggtc atgccttgag gagcaccagg ctggccagtc atcgccatcg cttgctgcat
     4321 ttgctggggg gtgcccatgt agggttggaa accggctttg gaggccgtgt cggcgtcaag
     4381 ggcgatttgt tggttggagt aggtgcccgc acgaaacagg gaccggtcgc ggtagtcctg
     4441 ggggagggcg gtgtacatcc cgctgtcgta ggcttgctgg agcatcgcgt ggttgccctt
     4501 cgacgcatag ccctcgactt ccttcatctc cactttgcgg cgttcttcga ggtccttcgc
     4561 ggcctgctcc tgctcgcgtc ggcgttgaga catctccgcg cccatgagga tagaggcgac
     4621 ccctgagatg ggacccagcg gcataccagc gaggaagggt ttgatgtcca ttagatggtt
     4681 cctgtgtagc tataggacgt ggtgggcatc attttcatca tcatctgcgt gccgttgttc
     4741 atcatggtgt tttggagctg ggtctggttg gcggcattgg cattgacgcg ggaggtttcg
     4801 gcgccggtca ctgcgctcat tccctgcatt ccagtggcgg tgttgccggt catggcgttc
     4861 atgacttggg gcaaaaacca ctggtagaaa ttgggaatgg tggaggcgac ctgctgctcg
     4921 cccgccatgg tctgatcaga ctttaggcgc tggccgaatg gcgtgccggc gagtcggctt
     4981 cggccgaggg agtcgttgag ggagttcatc gacttgctga agcccgacct cgcactgttc
     5041 agcagcgtgg tgagcatcgg gatcatttgt gagatcccgc cagtctggac gagttggttc
     5101 ccctggcccg ccagtgattg ctggagtgga ttccaggtgt cgaataggct ctgcgaccgg
     5161 ttctcctgtt ccttcgatgc gctgggttgt tgtgctttgt tgccgcccat gtcgtgcctc
     5221 ctcaaaaccg gcgcgggtga gggtgaggaa ccaggctggt tcgccgtccc aggccgccgg
     5281 gatagggtca gtgatagtgt agcctaattt ccggtgggcg tcgagtagtt ctgcctgctt
     5341 agttatacct acaagcaccg gccaagtgcg agtggcatgg tcgagcactt catagacgaa
     5401 tttgagggcc gaggcagagt ggcgggagtc ctcgcggagc caaatcgagg ccagcgcccc
     5461 gatcgtaaag ggttgaaacc aggcggcacg acagatccga cccccagcat cgagttggaa
     5521 tacgaggtca gtcggccgac gcatatctcg cataaatcgg ctcatggact gctcgtcggc
     5581 ggcgaacaat ttggtgaggt cgccagaggt tttgagctgc ccccaccagt cagctaccgc
     5641 gatgtcgtga tcggagtcag gcacgtaggg gtagatagtc gacatagtta gccccgccct
     5701 tgagatgctt gttggttggt cgagtttgag acccccatcg cgaggtctag ttccaccgcg
     5761 tagaggttga cgacatcctc gatctggccg gtgagtcgca acccaacgag acggcaggtg
     5821 cggttgatgg agtattccac cagttcgcgg gagttggtga ggaatgtagg taattctacg
     5881 gtcgtattat caattacgag tgttggcgta agtagttgtc cacctgtgtc aatatcgatg
     5941 tagacacgct gcaccaccgt gcgctgagag atgtcactga tcttggcgcc cagttcccat
     6001 tcgaggtcga tggcgtcagt gccatccaat acggtgctgt aatcctccag tgcatagacc
     6061 ccaaaattga aactcgcaat cacgttgggg gtgtcctgct cagtgtagag ggcggagcag
     6121 ccgatcccaa ggtctcgcca ggtcccgagt cgaaggttta ttgcgagggt ggtggtgcca
     6181 tcgctgataa tgtactcctc tttcgcgtag gtcgccgtcg tccctacaaa tgggccgagg
     6241 ttctcgatcg tctgcccgcg gaagatgggg ccgattgcct cgaagccgat cagcgtggag
     6301 ttgttgccgt cgaacaggcg gatcccgtcg aaggattggt agaccacccc gaacggtgtg
     6361 acgaccacgg tgtaagggta gatcgtgccg gggcattggt agactttgcg tgggatgaag
     6421 ggttcggcgt ccccagtgat gcggtaaatg ttcttttggg tcatgaccca gttggactcg
     6481 gcgaacgaga accccacctg gcaagggtca tctgtgttcg agacttccac aaaccccagc
     6541 aggctttctg gcctcccgat gggcgaatag taggcgcggc cggcggtgcc tggttgggtg
     6601 tcgcgggtca tccacatcac agagtcatga aaccaaactt cggcgcagga atcgtacggg
     6661 cggtcgttgt cggtttggag ttggaagctg agcaggtcag cgtcggccac gttatcggtg
     6721 gtggcggtgg tggagttatc gctgatcgtg atggcgagga aatagagcgc gccgccaacg
     6781 gtggtgcggt agatttcacg ttcggtgact tggccatcgc tcgacaccgg gatgttggtg
     6841 agggccacgg agcttgggcc actcgcgatg gtgatggagg cgtcgatgtc ctgggcattg
     6901 gagcgcgagc cggtcgaccc attcctgaat acgactttat acttatagac cccgttggcg
     6961 agtgcgccag tgcccgcgga ggtttcggcg agcgagggcg ggccgccggg gttggtgatc
     7021 ccccaggtgg tgacactgcc gccgtcgtcg agtttgaggg caagtccgcc accggtaata
     7081 aagaggtaat cgttgaggcc aggttgggcg ggcatgggca cgaaacggag tcggttgcca
     7141 ttgagggagc tggtgaccac cgcgcccgcg cgatacagcg acccccctgc ggcttgaatt
     7201 tgcacatcat caaatcgaaa cagcgagtgg gcggtgaggt cgtagagttt ggtgctgccg
     7261 tcgcgtgagc ggaggtgcgg ggtgcggatc gcgtggagcc ccttggcacg gcggatgtaa
     7321 ccggtcatgg tgtgctcttt gccgcccacc gaccagagcc ctttggagaa atcgtgccac
     7381 tctgctctca tttactatgg tacctccgca atcggcaccc agccttgtag ttctccacta
     7441 taaagcaaaa cccttgcgcc accaacaggc acggtgacat tggtacctgc gcgattgatg
     7501 aatcgattgc cgagggtgct ggcagaggag ttgtggatca ggggaagtga attggaggaa
     7561 cccgagacgt ttttgagaat gatgatgcga cccggttcgc cgccggtgag tccagtgagg
     7621 ccagcgttgg cggtggttat ggttaactcg aaatagtgtc cgcgaccgat tacgaggtta
     7681 ttgacaacat cagcgatgac ggggagggtg gtgcttgatg tcgggtcggc cgcacgagcg
     7741 gagacggaga gttcgtcgag gcgctggttg atggcgagga tgagggtgtc gaaggcgctg
     7801 gaggagtatt cctttggggc gcgccaggcg agtcgggagg ccatcagcta atcatccccc
     7861 acaacacgtc tgcgaattgt tgtgcgaagt ccgccagatc atcatcgcga tggttggatt
     7921 cgtgaccaaa ctcgcgtgac agcacctccc acatcaccgg cagttccata aattctagta
     7981 cgtctggggt gagttgggtg tgcaacagaa ggaccgcata tggtggatag tccaacccca
     8041 gtggtgagcc gaacataaaa ataaagttcc ccgaggcgcg gttgggggtg gggtagaggc
     8101 ggaaagtttt ggtggcttca tcctctaccg agtaggccac tgggagtccc tgctcgtcac
     8161 gccaggcggg gttgagtgat tccagcgcgc ggtgatccat gcgatcgagc cagcggtcgt
     8221 cgtagaacat gccgaggatt ttgagttggt cgtcggcgag ggcataagtc gatgtacctg
     8281 ctaccattgt gacgaggctg gcggtcacaa accacggcgc gcgcccggtg tcgtcgatga
     8341 tgtggtcgta gtattgttcc gcggtgaggg ggtcggcgtc acccagcgcg aaagtttgga
     8401 cgagatctaa aatactcgat ttactcattt agctcccaca agggggttga taggcgttgg
     8461 agaccagtgc gccatcacca ctcaatacct ctccgaattg atcgaggtag taggtttgtc
     8521 gaccatcgaa ctctaaaaag aacgggcgtc ctaagtactt gtacccgttg gttgtacacc
     8581 aca
//
LOCUS       DAORQN010000058         8524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_425_length_8524_cov_8.736451, whole genome shotgun sequence.
ACCESSION   DAORQN010000058 DAORQN010000000
VERSION     DAORQN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_425_length_8524_cov_8.736451"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PK614_05110"
     CDS             <1..385
                     /locus_tag="PK614_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRB81623.1"
                     /translation="REYVATRQKLLSRDEEELRNTEKQIKEQASKLTDNEKKEKETSF
                     RAKIQDYQKRAQEFNQELQGKQKELVDEYMKKIAVATQAVADKAGYQLVLDKGSEQTV
                     KIVIFNKDTIDLTEQVIKEFDRTNK"
     gene            complement(389..1459)
                     /locus_tag="PK614_05115"
     CDS             complement(389..1459)
                     /locus_tag="PK614_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRB81624.1"
                     /translation="MSFSRRELFNNAGFGLLLLPALMRSPRTILGHLLFDPDGPLVPL
                     KPIPDNPYRRNGRSIVAIVYGKDPRRMLPRAVDLLGGLDLMGMRGKRVLLKPNVLNDQ
                     PPPSTTNPQVVAAMAAMAKANGAAEVVVADGSGIIRLPTSANLTSTGMRAAAETAGAR
                     VLALEDEPWVKIEAPEAQALRQFYFSRPVYESDVVINMPVIKTHRFAEYSCSLKNFVG
                     AVHPRYRPSVTFWTGDWHERIAEINLAVHPALTVADGTTTMIEGGPTSGTPSKTDLVI
                     CSGDRVAVDLVAIALLRSFGTWPKLQGKRIGDQRQIKRAAALGLGVGSGQDIELVTEA
                     VDPAPPAFERLVEHIRRELLSV"
     gene            complement(1463..1843)
                     /locus_tag="PK614_05120"
     CDS             complement(1463..1843)
                     /locus_tag="PK614_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB81625.1"
                     /translation="MSHNPGFLKVVEEARGRVRECTVADVKARLDRAERFHFVDVRED
                     DEYALDHAAGAIHLGKGVIERDIETVLPDKHESIVLYCGGGYRSVLAADSIRLMGYTN
                     VISMDGGIKAWRAAGYPIEKGRRP"
     gene            complement(1854..2870)
                     /locus_tag="PK614_05125"
     CDS             complement(1854..2870)
                     /locus_tag="PK614_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRB81626.1"
                     /translation="MAVKLVSNPPNRFESTQREALEPSSRVEIQLFEDDTHQILSRND
                     SPDLPFRWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFDSKIVLKRRAADLLKQAF
                     EKRSWDGELIVFSGNTDCYQPLESTLGLTRACLEICAAYRNPVGIITKGALPVRDLDL
                     FRQLQEQAWIRVYFSIAFADDETARLVEPQAPTVSKRFETMRILSEAGIPTGISVAPI
                     IPGLNEEAIPELLSRAKSAGATSATCSLLRLPGHVETVFLDRMREALPDRISKIIHRL
                     QEVRGGKLSESGFFGRHHGNGTYWRCVEQLFELGRRRAGFVEDDRPVPGTFRRPTAQQ
                     SLFD"
     gene            2992..4278
                     /gene="serS"
                     /locus_tag="PK614_05130"
     CDS             2992..4278
                     /gene="serS"
                     /locus_tag="PK614_05130"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRB81627.1"
                     /translation="MYDLRVLRDNLDKLRDQLGSRGKDVAWEDLRTLTEDRRTRTIQV
                     EELRHQLKKGSDDVARLKREKQPADEAMAAMKALGETIKNHEEQLRVVEEQLAHIALR
                     IPNLPHASVPDGRDEADNIEVRRWGTPPQLSAPAQSHWDLGEALGILDFDRAVRMAKA
                     RFAVLTGLGARLERALINYMLDMHTTQHGYREVLPPLLVNRTAMTGTGQLPKFEDDLF
                     QLRDEELFLIPTAEVPVTNLHREEILAEESLPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFVRPEQSYDELERLTSHAEAILQGLGLHYRVITLCKGDMGFSAAKTY
                     DIEVWLPSQQTFREISSCSNFEAFQARRANIRCRAKAGKKDNKPEFVHTLNGSGLAVG
                     RTLVAILENYQQPDGTVTVPTVLQPYMGGIQTIERS"
     gene            4304..4393
                     /locus_tag="PK614_05135"
     tRNA            4304..4393
                     /locus_tag="PK614_05135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4338..4340,aa:Ser,seq:tga)
     gene            4519..5271
                     /locus_tag="PK614_05140"
     CDS             4519..5271
                     /locus_tag="PK614_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRB81628.1"
                     /translation="MLDLVGLGILAGGVPVYLGIAVALVLRRALPRTWESGLIGLSVG
                     VLTYLFFDLMHEAVELMGARDPFSWLLFLGSLFLSFVGLVHLEKYQFLKGRHADQALT
                     LPFVIAAGMGLHNLGEGLAIGTSYAGGEWALSGLLVAGFALHNGTEGFGIVGAAGQSP
                     IPPRTVVLLGLTGGAPTCIGTVLSGLTVSPYLSLVWYALAAGSLLYVVFALVAMTYTS
                     SRKVHMAYGVWTGIALMAMTAMFLTLVGGHRS"
     gene            5491..5997
                     /locus_tag="PK614_05145"
     CDS             5491..5997
                     /locus_tag="PK614_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRB81629.1"
                     /translation="MHQDRRARTIDTSIIGIVSSGSPPLSIKLSQQRPLTHSLRTKPQ
                     PFWPTPQITPLNSVTPAARLSALLSKRSGDRLTLKELSTLLGYSEKYTSEIFRKYMGL
                     SFSEYLKQLHLAKATTLLKDQNLTIAQIAESVGFSDAFAFSHFFKRAIGCSPSEFRKQ
                     QTPEAGLR"
     gene            5994..6917
                     /locus_tag="PK614_05150"
     CDS             5994..6917
                     /locus_tag="PK614_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB81630.1"
                     /translation="MNDNHESLARMGGWLVSLTLHGLSLGTALVLAAEFSILPEPRPF
                     RWDVTMVSAPALQPVAADVPTPFSASSVPPSVMPRDKSPRQARSRPNAAHTEAARPLE
                     AHSGIISPAQDGFVRPQATETPSLAVDSVSPVPHTPSADLTSDELVHEAPQVTRVDLP
                     VTTTAPSEIPPPRVDAAEVSLTTSMPSIRESIRLVNRPTPRAREATVSRILHADYGWL
                     AEMLFAKVEQSKRYPTFAKSRRWQGNVMLQAVIHEDGSISDITIVTPSGHSILDLDAV
                     ALLEQASPVQLKYPLGQSHVVVHIPIGYRLE"
     gene            6926..7447
                     /locus_tag="PK614_05155"
     CDS             6926..7447
                     /locus_tag="PK614_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB81631.1"
                     /translation="MQNEHTLPCLTAFEEHHNDLLRFFTHKLGCRDLAADCTQDTYMH
                     LVRMRPSIDVQNPRAFLFRVAANLAVDNLRKLRTRRGALSIEPPPEETASPAPSAEDT
                     LDAKQRVAQLEVAMRELSPKCRRALLLNRLEGKTHREIADSLKVSESMVAKYIVQALR
                     HCRAHLHPEESTR"
     gene            7546..>8524
                     /locus_tag="PK614_05160"
     CDS             7546..>8524
                     /locus_tag="PK614_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRB81632.1"
                     /translation="MPDSTPPDASSPLIDEALAWLVRIHSGHASDEERRACTAWRTQS
                     SAHHLAYTEAEILWRDIGLVPNGDCAGSADLTASMPHGTLPRARRTMKWGLAACVLLA
                     AGWLSWGVVQERLDLASADYRTAVGEQRNIRLEDGSRIHLNTDTAVAVAFTPQQRTIR
                     LLTGEASFTVTPETVRPFTVQSGDVSTRALGTSFLVQRHQDAVTVTVTEHAVQVSATD
                     GRQLPAAVVRQSEQISYSAEAGLGLAHAVDSNAITAWQRGKMIFEAQPLAAVVEELNR
                     YRAGRIMILNPALRSLNVTGVFDIGDPDAALRMIEQTLRIHDTTLTPYLV"
BASE COUNT         1831 a         2645 c         2363 g         1685 t
ORIGIN      
        1 gcgggagtat gtggcgacac gccagaagct cctgtcccgg gatgaggaag agttacgcaa
       61 tactgaaaag cagatcaagg aacaggcctc caaactgacc gacaacgaaa agaaagagaa
      121 ggaaacctcc ttccgtgcca aaattcagga ttaccagaag cgcgcccagg agtttaatca
      181 ggaactgcag ggcaagcaga aggaactcgt cgacgaatat atgaagaaga ttgccgtcgc
      241 cacccaggcc gtcgccgata aagctgggta ccagctggtg ctggacaagg gcagtgagca
      301 gaccgtgaag atcgtgatct tcaacaaaga caccatcgat ttgaccgagc aggtcatcaa
      361 ggaattcgac cgcacgaaca agtaatactt atacgctgag cagctcccgc cgaatatgct
      421 ccaccagccg ttcaaacgct ggtggagccg ggtccaccgc ctccgtaaca agctcaatgt
      481 cctgccctga tccgacccct agtcccaacg ccgccgcgcg cttgatttgc cgctgatccc
      541 cgattcgttt gccctgcagc ttcggccagg tgccgaagga ccgcaacagc gcgatggcca
      601 ccaggtccac cgcgacccgg tcaccgctgc agatcaccag atccgttttc gacggggtgc
      661 cggatgtcgg acccccttcg atcatggtcg tcgtcccgtc ggccacggtc agcgcggggt
      721 gcacggccag attgatctcg gcaatgcgct cgtgccagtc accggtccag aacgtcaccg
      781 atgggcgata ccgcggatga accgccccca caaaattctt gaggctacag gaatactcgg
      841 caaaccgatg ggtcttgatg accggcatat tgatcaccac atcggactcg tagacgggcc
      901 gggaaaaata aaactgccgg agggcctgag cctcaggggc ctcgatcttc acccagggtt
      961 catcttccaa cgccaggacg cgggccccgg ccgtttccgc cgcggctcgc atgcccgtgc
     1021 tcgtcaggtt ggcggacgtg ggaagccgga tgattcccga tccatcggcc accaccactt
     1081 cggcggcccc gttcgctttg gccatggccg ccatggccgc gaccacctgc ggattcgtcg
     1141 tggatggagg cggctggtcg ttcaacacat tgggcttcag cagcacgcgt ttcccgcgca
     1201 tccccattaa atcaagtcct cccagcagat cgacggcgcg tggaagcatg cgacggggat
     1261 ccttgccgta cacgatcgcg acgatcgacc ggccgttacg ccgatacgga ttgtcgggga
     1321 ttggtttgag cggaacgaga gggccgtccg gatcgaacag cagatggccg agaatggtcc
     1381 tgggggagcg catgagggcc ggtagcaaga ggagaccgaa ccccgcgttg ttgaacagtt
     1441 cgcgcctgga aaatgacatg cgctacggac gccggccctt ctcgatggga tacccggcgg
     1501 cgcgccaggc cttgattccc ccgtccatcg agatgacatt ggtgtagccc atgaggcgta
     1561 tgctgtccgc agccagcacc gaccggtacc cgcccccgca atacaacacg atggactcgt
     1621 gcttatcagg cagcaccgtt tcaatgtccc gctcgatgac acccttgccg aggtgaatcg
     1681 cccccgccgc atggtccagc gcatattcat catcttcgcg gacgtccaca aaatgaaacc
     1741 gttccgcgcg atccagtctg gccttcacat cggcgacggt acattcgcgc acacgtcccc
     1801 gtgcctcctc cacgactttc aaaaaccccg ggttatgact cacgactctc ctcctaatcg
     1861 aacaatgatt gctgcgctgt cgggcggcgg aaggttccgg gaaccgggcg gtcgtcctcg
     1921 acgaatccgg cgcgacggcg acccaactca aacaactgtt cgacgcaacg ccaataggtc
     1981 ccattgccat gatgccggcc gaagaacccg ctctcgctca gcttcccccc acgcacctct
     2041 tgaagtcggt ggatgatttt cgagatccgg tctggaagcg cctcgcgcat gcgatcgaga
     2101 aacaccgttt ctacatgccc cggcaaacgc agcaagctgc aggtcgccga ggtcgctccg
     2161 gctgatttgg cacgagacaa cagctcaggg atggcctctt cattcaagcc tggaatgatc
     2221 ggcgccacag atatgccggt gggaataccg gcctctgaga gaatgcgcat cgtctcaaag
     2281 cgtttactga cggtcggcgc ctgcggttcg accagacggg ccgtctcgtc gtcggcaaag
     2341 gcgatgctga aatacacacg gatccaagcc tgttcctgca gttgccggaa cagatcaaga
     2401 tcgcgaaccg gaagcgcacc cttggtgatg atcccgacag gattccggta ggccgcacag
     2461 atttcaaggc aagccctggt caggccaagg gtggattcga gaggttgata acaatcagtg
     2521 ttgccggaaa acacaatcaa ctcaccgtcc caggatcgct tctcaaacgc ctgcttgagg
     2581 agatccgcgg cacggcgctt cagtacgatc ttagagtcga agtcggtgcc tgcgccgaat
     2641 ccccagtatt catgcgatgg cctggcatag cagtaggcgc aggcatggaa acagccacga
     2701 tagggattca cgctccatcg aaacggaagg tccgggctgt cattccggct cagtatctgg
     2761 tgggtgtcgt cttcaaaaag ctgaatctcc acacgcgacg agggttcaag cgcctcacgc
     2821 tgcgtggatt caaagcgatt gggtgggttg gatacaagct tcacggccac tatggtgccg
     2881 ccctccccac ccgttgtcaa ttgcctttcc ccggcctcgc ttggcgaccg gtttcattga
     2941 ctacgcggac atccagtgtt agatttctcg ccgcttcaga ggggagagtc tatgtacgac
     3001 cttcgtgtat tgcgggacaa cctggacaag ctccgtgacc aattgggttc acgcgggaag
     3061 gatgtcgcgt gggaagatct gagaacactc acggaagatc gtcgaacccg caccatacag
     3121 gtcgaagaac tacggcacca gctgaaaaag ggatcggacg acgtcgcccg gctgaagcga
     3181 gagaagcagc cggccgatga ggccatggcg gcgatgaagg ccttgggaga gacgatcaaa
     3241 aaccacgagg aacagcttcg tgtcgtggaa gaacagttgg cccatatcgc gctgcgcatt
     3301 ccgaacctcc ctcacgcctc tgtcccggat ggccgggatg aggctgacaa tattgaagtg
     3361 cgccgatggg gaacgccgcc gcagctctcg gctccggccc aatcccactg ggatcttggc
     3421 gaagcgctcg gcattctgga tttcgaccgc gccgtgcgca tggccaaagc ccgctttgcc
     3481 gtactgaccg gattgggagc ccgactcgag cgggcgctca tcaattacat gctggatatg
     3541 cacaccacac agcatggcta ccgggaagtg ctgccgccgc tgctggtcaa tcgcacggcc
     3601 atgaccggga ccggacaatt gcccaagttc gaggacgatc tgtttcaact acgcgatgaa
     3661 gaactcttcc tgattccgac cgcggaggtg ccggtcacca atctgcaccg ggaagagatc
     3721 ctggccgagg aatcgttacc gatccggtat accgcctata ccccttgctt tcgacgagaa
     3781 gcgggatctt acgggaagga cacgcgcggc ctcatccggc tccaccaatt caacaaggtc
     3841 gagctcgtcg cgttcgtaag accggaacaa tcctatgatg agctggagag attgacctca
     3901 cacgccgagg ccattctgca ggggttggga ctgcactatc gagtgatcac actgtgcaaa
     3961 ggggacatgg ggttttctgc cgcgaagacc tacgacattg aggtatggtt gccgtcgcag
     4021 cagacgtttc gcgaaatttc ctcctgcagc aatttcgaag cgttccaagc gcggcgggca
     4081 aacattcgct gccgggcaaa ggccggaaag aaagataaca agcctgaatt cgttcataca
     4141 ctgaacggat ccgggttggc ggtgggaaga acccttgtcg ccattcttga aaattaccag
     4201 caaccggatg ggacggtgac cgtcccgaca gtgctacagc cctacatggg tggaattcag
     4261 acaatcgaac gatcctgacg ctgagtgtgc tacggcctgg tgtggagggg tgacggagtg
     4321 gccgaacgtg ccggtcttga aaaccggaga tggggtaacc tatccgcgag ttcaaatctc
     4381 gccccctccg ccatatttcg atagacgagc gcctcactcg aaccagcctc tcgcactctc
     4441 acacctattt tcttcgcacc gagcagtttc cggatactga acattccacg cacgacagat
     4501 attggctagg attgccccat gctcgacctc gtcggattgg gtatcctcgc aggaggcgtt
     4561 ccggtttacc ttggcatcgc ggtggccttg gtgcttcgcc gtgctcttcc ccgcacatgg
     4621 gaaagtggcc tgatcgggct ctctgtcgga gtactgacct atctcttttt cgatttgatg
     4681 cacgaagcgg tggaattgat gggagcccgc gatcccttct cgtggctgct gtttctgggc
     4741 agcctgtttc tcagctttgt cggccttgtc cacctcgaaa aatatcaatt cctcaaagga
     4801 cggcacgccg accaggcact gacacttccg tttgtcatcg cggcgggcat gggtcttcac
     4861 aacctgggtg aaggactcgc gatcggaacc agttacgccg gaggagaatg ggccttgagc
     4921 ggactcctcg tggctggttt cgccctccac aatggaaccg aaggattcgg cattgtcggg
     4981 gcggcggggc aatctccgat tcctccgcgc acggtggtgt tactcggctt gaccggtggc
     5041 gcgcctacat gtatcggaac ggtcctaagt ggactcaccg tgtcgcccta cctttctctc
     5101 gtctggtatg cgttagccgc cggatcgctc ttatatgtcg tgtttgcatt ggtggcgatg
     5161 acatacacga gctcccgcaa ggtccatatg gcctatggtg tctggacggg tattgcactt
     5221 atggcgatga cggccatgtt tctgacatta gtcggcggac atcgctccta atcctccgct
     5281 ccgttccgaa caaattttca aacgatcact cggtggcctg aagctttgct ccacagcccg
     5341 cttgacatgt tttcctaaat tgtgatattg agaatcgtac tcaataccga gatagtctct
     5401 gccagtactc tcagcagagg cccgaggaat ttaattccca gagcccacgg tcatacgatc
     5461 gcgggctttt tattttttgt gggggaaatt atgcaccaag accgccgggc acgaactatc
     5521 gatacatcca tcatcggcat cgtctcatcc gggagccctc cgctttcaat caaattgtca
     5581 cagcaacggc cattgaccca cagcctgcgg accaagccac aacctttctg gccgacgcca
     5641 cagatcacgc ctctcaattc cgttaccccg gcggcacgct tgtcggccct gctgtccaag
     5701 cgcagcgggg atcgtctgac gttgaaggaa ctctccactc tcctggggta ctcggagaaa
     5761 tacacctccg aaatctttcg caagtatatg gggctctcct tttccgaata cctcaagcaa
     5821 ctgcatctcg ccaaagcgac gacactcctc aaggaccaga atctcacgat tgcccagatt
     5881 gcggaatcgg tgggattcag cgacgcgttt gcgttcagcc acttcttcaa acgagctatc
     5941 ggatgttcgc cgagcgaatt tcgaaaacaa cagacgccgg aagcaggttt gagatgaacg
     6001 acaaccacga gtctctggcg cgaatgggag gatggctggt ttcgctgact ctgcatggcc
     6061 tgagtctcgg gacggcgttg gtcctcgcgg ctgaattctc cattctcccg gagccgcgcc
     6121 ccttccgctg ggacgttaca atggtgagcg cgccggcgtt gcaacctgtc gcagcggacg
     6181 tacccacccc cttttccgca tcgtccgtcc ccccttccgt catgccgagg gacaagtctc
     6241 cacggcaagc ccggtcccgt ccgaacgccg cccataccga agcggctcgc ccgctggagg
     6301 ctcattccgg aatcatctca cctgcgcaag atggattcgt ccgtccacaa gccaccgaga
     6361 ccccctccct ggccgtcgac agtgtctcgc ctgtccctca tacaccatca gcggacctga
     6421 catccgacga actagtgcac gaggcgcctc aagtcacacg tgtagatctt ccggtcacca
     6481 cgacggcgcc gtccgaaata ccgcctccga gggtcgatgc agccgaagta tccctcacga
     6541 cctccatgcc ttccattcgt gaatcgatcc ggctcgtcaa ccgtccgacg cctcgcgccc
     6601 gcgaggccac cgtctcacga atactccatg ccgattacgg atggctcgcc gaaatgttgt
     6661 tcgcaaaggt cgaacagagc aaacgttacc caacattcgc caaaagccgt cgctggcagg
     6721 gcaatgtcat gcttcaagcc gtgatccatg aagacggcag catcagcgac attacgatcg
     6781 tcacgccatc gggccactcg attctcgatc tcgacgccgt cgctctgctc gaacaagcgt
     6841 ctccggtcca gttgaagtat ccactcggtc aatcccatgt tgtcgtccac atccccatcg
     6901 gctatcgcct ggaataggat ccatcatgca gaatgaacat acacttccct gtctcactgc
     6961 cttcgaagaa catcacaacg atctcttgag gttcttcacc cacaagctcg gctgccgtga
     7021 tctcgccgcc gattgcactc aggataccta catgcacctc gtgcgcatga ggccttcaat
     7081 cgatgtgcaa aacccgcgag ccttcctttt tcgggtcgcg gccaatctcg cggtagacaa
     7141 tctacgaaaa cttcgcaccc gtcggggggc gctcagcatc gaaccgcccc ctgaagaaac
     7201 agccagtccg gccccgtcgg ccgaggatac cttggatgcc aagcagcggg tcgcccaatt
     7261 agaagtcgcc atgcgcgaac tgtcgcccaa atgccggcgg gccttgctac tcaacaggtt
     7321 ggaagggaaa acacaccggg aaatcgccga cagtctgaag gtgtccgaga gtatggtcgc
     7381 gaaatatatc gtccaagcct tgcggcactg ccgcgcccat ttgcatccgg aggaatccac
     7441 cagataatcc taactgttga aagccgagcg ccatccgtct tatagagtag cgtacatcga
     7501 ccagtatccc gttcaggggc agtcgtgtgc acaatggtat gatggatgcc tgactcgaca
     7561 cctccagatg cctcctctcc actgatcgac gaagccctcg cttggttggt gcgcatccat
     7621 tccggtcacg cctccgatga agagcgccgg gcgtgcactg cctggcggac gcaaagttcc
     7681 gcccaccacc tcgcctacac agaggcggaa atcctatggc gggatatcgg cttggtgccg
     7741 aatggcgatt gcgccggttc ggccgacctg acggcatcga tgccgcacgg cacgctcccg
     7801 cgtgcacgac gcacgatgaa gtggggactg gccgcttgtg ttttactcgc cgccggttgg
     7861 ctctcatggg gagtcgttca agaacgactc gacctcgcgt cggccgatta ccgcaccgcg
     7921 gtgggggaac aacgaaacat caggcttgag gacgggagtc gtatccatct caacaccgat
     7981 accgcggtgg ccgtcgcctt cactccgcag cagcgaacga tccgactgct caccggagaa
     8041 gccagtttta ccgtcactcc cgagacagta cggccgttca cggtacagag cggagatgtg
     8101 agcacccgcg cgctcggaac cagctttctg gttcagcgcc accaagatgc cgtcaccgtg
     8161 accgtcacgg agcatgccgt tcaggtatcg gcaactgacg gccgccagct ccccgcagcg
     8221 gtggtccggc aaagcgagca gatctcctac agtgccgagg ccggactggg tctggcccac
     8281 gccgtcgact ccaatgccat cacagcctgg caacgtggga aaatgatctt cgaagcccag
     8341 ccgctggccg cagtcgtcga agagctcaac cgataccgag ccgggcgcat tatgattctg
     8401 aacccggcgc ttcgctcact gaacgtgaca ggtgtgttcg acattggcga cccggatgcc
     8461 gcgctccgaa tgattgaaca gacgttgcga attcacgaca ccaccctgac gccctacctc
     8521 gtcc
//
LOCUS       DAORQN010000059         8348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_430_length_8348_cov_645.265887, whole genome shotgun sequence.
ACCESSION   DAORQN010000059 DAORQN010000000
VERSION     DAORQN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_8348_cov_645.265887"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1049)
                     /locus_tag="PK614_05165"
     CDS             complement(48..1049)
                     /locus_tag="PK614_05165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRB81633.1"
                     /translation="MIKAMKDFQIPMRVEVDKDTLSPTFGRFTTEAFERGFGTTVGNS
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNVKSLRLALQGDKPKTI
                     RLKKKGPGEAKGSDITHDGDVTILTPDLHIATLDKDASLDLEMTIKHGRGFVPAERNK
                     EEGLPIGVIAVDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTISPRDALSNA
                     AGILREHLDIFINPEERSDAKPAVGSEDLSDEVNKNLYRSVNELELSVRAANCLKNAN
                     IKTIADLVQKTEAEMLKTKNFGKKSLNEIKEILTEMGLSLGMKVDALPSGDAGVRSE"
     gene            complement(1133..1759)
                     /gene="rpsD"
                     /locus_tag="PK614_05170"
     CDS             complement(1133..1759)
                     /gene="rpsD"
                     /locus_tag="PK614_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRB81634.1"
                     /translation="MAKYSGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARQRTSDYSLQLREKQKLKRIYGLQECQFRGIFERAERQAGVTGDTLLRLLECRLDN
                     VVYRLGFGASRKEARQLVNHGHLMINGRKVKAPGALVKAGDSVEVRQKSRELLPIQGA
                     LAAVDGRGIPEWLELDRAACKGTIRSLPTKEHIVLPVNEQMVVELYSR"
     gene            complement(1841..2224)
                     /gene="rpsK"
                     /locus_tag="PK614_05175"
     CDS             complement(1841..2224)
                     /gene="rpsK"
                     /locus_tag="PK614_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRB81635.1"
                     /translation="MSVKKGKKKERKIVQSGVAHVQASFNNTIVTISDMSGNTVVWAS
                     SGNQGFKGSRKSTPFAAQRAGEAAARKAMENGMRQVDVYVNGPGAGRESAIRSLQSAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(2266..2655)
                     /gene="rpsM"
                     /locus_tag="PK614_05180"
     CDS             complement(2266..2655)
                     /gene="rpsM"
                     /locus_tag="PK614_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRB81636.1"
                     /translation="MARIAGVDLPKDKRIDIGLTYVYGIGRVAAQAILKKAGVDGSIR
                     VKDVSEDKIVKIREVIERDYRVEGDLRKEVSMNIKRLVDTGTFRGLRHRKGLPVRGQR
                     TKTNARTRKGRRSGVGSKPQKPAGSRA"
     gene            complement(2789..2902)
                     /gene="rpmJ"
                     /locus_tag="PK614_05185"
     CDS             complement(2789..2902)
                     /gene="rpmJ"
                     /locus_tag="PK614_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRB81637.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRVLCANPRHKQRQG"
     gene            complement(2921..3139)
                     /gene="infA"
                     /locus_tag="PK614_05190"
     CDS             complement(2921..3139)
                     /gene="infA"
                     /locus_tag="PK614_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRB81638.1"
                     /translation="MPKEDVIEVTGTVAETLPNAMFRVELEHGHRILAHISGKMRMHF
                     IRILPGDKVTVQLSPYDLTRGRITYRFK"
     gene            complement(3161..3916)
                     /gene="map"
                     /locus_tag="PK614_05195"
     CDS             complement(3161..3916)
                     /gene="map"
                     /locus_tag="PK614_05195"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRB81639.1"
                     /translation="MIVFKTPDEVSLMAQASHVVAEVLELLKEKVAPGITTDDLDRMA
                     EEAIRVRGAIPAFKGYRNYPKTLCASVNEQVVHGIPSKRRLKEGDIIGLDLGAIVSGF
                     YGDSAVTVPVGVASSEALRLIQVTEESMYRGIAKAVVGNRLSDVSHAVQTHVEQAGFA
                     VVTEFVGHGIGRQLHEEPQVPNYGRPGQGPRLQVGMVLAIEPMVNMGSSAIRILEDRW
                     TAVTHDGRWSAHFEHTIAIQPNGPARILSQLSH"
     gene            complement(3913..4569)
                     /locus_tag="PK614_05200"
     CDS             complement(3913..4569)
                     /locus_tag="PK614_05200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRB81640.1"
                     /translation="MRLVFLGAPGVGKGTQADRVTAQFGWPKISTGDLLREAVRNQTA
                     LGIEAKKSMDAGKLVPDNVVIGMVRAKVAEPMCQNGFILDGFPRTVPQAEELSGILAE
                     RGVSLDRVVNFRVSREDVIRRLSGRRSCPKCQSVFHVDFAAPKIEGQCDRCGASLVQR
                     SDDKPETIEARLKVYDEQTAPLIAYYEKKKVLSDLDGSGDMTHVYDRLSRVLAGLGAK
                     "
     gene            complement(4579..5892)
                     /gene="secY"
                     /locus_tag="PK614_05205"
     CDS             complement(4579..5892)
                     /gene="secY"
                     /locus_tag="PK614_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRB81641.1"
                     /translation="MFERLLTSFQNIFKIPELRTRVLFTLGMLIVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVIPHLS
                     KLAKEGERGRKKIIQYTRFGTIVIALIQGFGIAVGLEQMNQGAFVLSPGWGFRLMTVI
                     TLCAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTFDLYKVGQLSFPLL
                     VVLTLIMFGVVAAIVFLESGRRKVPVQYAKRVIGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWIKAIGSQLAPGSLLYTLMYVGLIVFFCFFYTAVVMNPV
                     DMADNLKKYGGFVPGIRPGQRTSDYIYSVLTKITFAGAIYLAIVCVIPEFLIYKLNMP
                     FYFGGTSLLIVIGVGLDTAQQIESHLLNRNYDGFLQKGKLRGRTT"
     gene            complement(5885..6334)
                     /gene="rplO"
                     /locus_tag="PK614_05210"
     CDS             complement(5885..6334)
                     /gene="rplO"
                     /locus_tag="PK614_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRB81642.1"
                     /translation="MKLHDLAPSRGAKHKRKRIGRGPGSGHGKTATKGHKGLKARSGG
                     GKRPGFEGGQMPLIRRVPKYGFTNQFRTEYTIINLKSLADLETTEAITPQLLMNEGLV
                     RRKGELIKILAQGELTKPLVVQAHKFSKSAEAKIQAAGGRAEVIPCV"
     gene            complement(6331..6540)
                     /gene="rpmD"
                     /locus_tag="PK614_05215"
     CDS             complement(6331..6540)
                     /gene="rpmD"
                     /locus_tag="PK614_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRB81643.1"
                     /translation="MATEKKSTIAQASLRITLKRSPIGTPHKHRLVLRGLGLRKLNAT
                     VLRPASDQVKGMIAKVGYLLEVSPQ"
     gene            complement(6540..7043)
                     /gene="rpsE"
                     /locus_tag="PK614_05220"
     CDS             complement(6540..7043)
                     /gene="rpsE"
                     /locus_tag="PK614_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRB81644.1"
                     /translation="MRVNPEELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGQGY
                     VGVGKGKAAEVPVAISKAVEQAKKHLVRVPIKGGTIPHEVHGLFGAEHVLLKPAADGT
                     GIIAGGAVRAVVELAGAHNIIAKTLGRGNPFNAVRATLHGLQQLCDPQEMMRLRRSAG
                     SETQVHV"
     gene            complement(7118..7483)
                     /gene="rplR"
                     /locus_tag="PK614_05225"
     CDS             complement(7118..7483)
                     /gene="rplR"
                     /locus_tag="PK614_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRB81645.1"
                     /translation="MNTQEKNRKLARRKQRVRKSVIGTSERPRLNVFRSRAHIYAQII
                     DDLRGHTVAAASTLDESLRKTVKSTGSIEGAKAVGKLIAERAKAAKVSMVVFDRGGRQ
                     YHGRVKALADASREGGLQF"
     gene            complement(7493..8029)
                     /gene="rplF"
                     /locus_tag="PK614_05230"
     CDS             complement(7493..8029)
                     /gene="rplF"
                     /locus_tag="PK614_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRB81646.1"
                     /translation="MSRIGRKPISVPAGVDIKVAGRVVSVKGPIGKLDWKLTDGLSVA
                     VNDGQLVVNRSGDARQIRAMHGLVRAELSNIIQGVTKGYEKALEITGVGYKAQLQGRE
                     MSFNVGYINPVTYTVPAGIDVKVDKQTLISIKGVDKRLVGQVAANIRSIKPPDVYKQK
                     GIRYAGEVLRKKAGKTGK"
     gene            complement(8086..>8348)
                     /gene="rpsH"
                     /locus_tag="PK614_05235"
     CDS             complement(8086..>8348)
                     /gene="rpsH"
                     /locus_tag="PK614_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRB81647.1"
                     /translation="LGYGEFQEDGHPYFSVQLRYVEEARPMITGMRRISKPGRRVYVG
                     RDDVPKVRNGIGVAVISTSKGLMTDSDSRRSGLGGEVLCSVW"
BASE COUNT         1957 a         2532 c         1882 g         1977 t
ORIGIN      
        1 agctgtcgac ccttcttttt gtggcgcacg gtagtcctgt tcctttctta ctctgaacgt
       61 acgcccgcgt cgcctgacgg caaggcgtcg actttcattc caaggctcag tcccatttcg
      121 gtcaggattt ccttgatttc gttgagggat tttttcccga aattcttggt cttcaacatt
      181 tctgcttctg ttttttgcac caagtcggca atcgtcttga tgttggcatt cttcaggcaa
      241 ttggctgccc gaacggacaa ttccaactca ttcacgctgc gatagagatt cttattcact
      301 tcatccgaca aatcttcact gcccacagcc ggtttcgcat cagagcgctc ttcaggatta
      361 atgaagatat ccaaatgttc gcggaggatg cctgccgcgt tggacaacgc gtcacgcgga
      421 gaaatcgtgc cgtccgtcca aatttcaagc gtcagcttgt catagtcggt catacgaccc
      481 acacgcgcgt tctcgacatg gaaattcacc cgcttgatcg gggagaaaat cgagtccacg
      541 gcgatcaccc cgatgggcaa tccttcttct ttatttcgtt cagccggcac aaatccacgg
      601 ccatgcttga tcgtcatttc caaatctagc gaggcatctt tgtccagagt tgcaatatgc
      661 agatcaggag tgagaatcgt gacatcccca tcgtgcgtaa tatcagagcc cttcgcttcc
      721 ccaggccctt ttttcttgag ccggatggtc tttggcttgt cgccctgcaa cgccaaccgc
      781 aggcttttca cattcaaaat aatggatgtg acatcttctg tcacgccagg aatcgtggag
      841 aattcgtgca acactccttc gatcttgacg gtggtcactg cagcccccgt caaagaagac
      901 agcaacacac gacgcaggga gttccccacc gtggtgccaa atcctcgctc aaatgcctcg
      961 gtcgtaaatc tgccgaatgt cggggaaaga gtgtccttgt cgacttccac ccgcatgggg
     1021 atctgaaagt ctttcatcgc tttaatcatg actccccctt cattaactcg tgtgtgccca
     1081 tctatcgttc agccatggct ggatgagcaa gccgccatcc agccacgtat tcttagcgcg
     1141 agtacaattc cacgaccatc tgttcgttga ccgggagcac gatgtgctcc ttggtcggca
     1201 acgaacgaat ggtgcccttg caggctgctc ggtccaattc aagccactcc ggaatacccc
     1261 ggccatccac ggccgccagt gctccttgaa tggggaggag ttcacggctc ttctgccgga
     1321 cttccacgga atcccccgct ttgacaaggg caccgggcgc cttcactttg cgaccgttga
     1381 tcatcaagtg accatgattg accaactgcc gggcctcttt ccgagaggct ccaaagccga
     1441 gccgatacac cacgttatcc aagcggcatt ctaggaggcg cagcaatgta tcgccagtga
     1501 cgccggcttg ccgttccgcc cgctcaaaaa tgccacggaa ttggcactct tgaagtccgt
     1561 aaatcctctt gagcttctgc ttttcacgca actgcagact ataatccgac gtccgctgac
     1621 gagcctgacc atgctgccca gggggatagc tccggcgctc aatcgcgcac ttctcggtca
     1681 tgcaacgaga cccttttaaa aagagcttct cgccctctct ccgacacaag cggcagacag
     1741 gtccactata ttttgccacg ctactacccc tcctctacct caacgactca agtacgacta
     1801 aatcagcgat taccttgttc tgatgacgtc ggaaccacaa ctagacacgt cggcgcttcg
     1861 gaggccggca tccgttgtgg gggattggcg tcacgtcgcg aatcagattg atgcgcaacc
     1921 cggcgctttg caatgaacga atggcggact cgcggcccgc ccccggcccg ttgacataca
     1981 catcaacctg ccgcatgccg ttctccatgg cctttctcgc cgccgcctct cccgctcgct
     2041 gcgccgcaaa cggcgtgctt ttacgggagc ctttaaaccc ttgattccct gagctggccc
     2101 acaccacggt gttcccgctc atatccgaaa tagtcacaat cgtgttattg aacgacgctt
     2161 gaacgtgagc gactccgctc tgaacaatct tccgttcttt cttcttgccc ttcttcacac
     2221 tcatagaatc tccctcagcg catggcgcct gctcggtcta atctcttatg ctcttgaacc
     2281 agcgggcttc tgcggtttgc ttcctacacc ggagcggcgc cccttgcggg tacgcgcatt
     2341 ggtcttggtc cgctgtccac gaacaggcaa ccccttgcga tgccggagcc cacgaaatgt
     2401 tccggtatcc accagtcgct tgatgttcat cgacacttct ttacgcaaat cgccttccac
     2461 gcgatagtca cgttcgatga cttctcgaat cttgacgatt ttgtcttcac tgacatcctt
     2521 cacgcgaatg gatccgtcga ctccggcctt tttcaaaatc gcctgagcgg caacacggcc
     2581 aatcccgtac acataggtca gaccgatatc gatccgtttg tcttttggca aatccacgcc
     2641 cgcaatacgc gccataatta ccccctaatt ccttcccttg attgcaccgt gagctggctt
     2701 gaaggacctt cgcaacaccg ttccgatgtc gccatgacca gcctcttgca ggctcgacat
     2761 acccctgcca aggcaaccca cattccggtc aaccctgtcg ctgcttgtga cgcggattgg
     2821 cgcataacac ccgaacgaca ccacgccggc ggaccacttt acatttcgca caaatcggct
     2881 tgactgacga cttgaccttc atagcaattc catttctcca ctacttgaag cgataggtaa
     2941 tccggcccct ggttagatca tagggcgaca attgcacagt caccttatcg ccaggaagaa
     3001 tacggatgaa gtgcatccgc atttttccag agatgtgcgc taaaattctg tgcccatgtt
     3061 ccagttctac gcgaaacatt gcattcggga gtgtttcagc caccgtgccg gtcacttcta
     3121 tgacatcttc ttttggcacg tatcgatacg cctcccagtc ttaatgtgat aactgtgaca
     3181 gaattcgggc cgggccattc ggctgaatgg cgatcgtgtg ctcgaaatga gcggaccaac
     3241 gcccgtcatg agttacagcc gtccatcgat cttctaaaat acgaatcgca ctgctgccca
     3301 tattcaccat gggttcaatc gccaacacca tcccaacttg aagacgcggc ccctgccctg
     3361 gccgtccata atttggaacc tgaggttctt catggagctg ccgtcctata ccatgaccga
     3421 cgaattcagt caccacggca aagccagcct gctcaacatg agtttgcacc gcgtgtgaca
     3481 catcagaaag ccgattaccg accacggctt tcgcgatgcc acggtacatc gactcttcgg
     3541 tcacctgaat cagccgcaga gcctcgctgc ttgccacacc aaccggcacc gtcacagcgg
     3601 aatcaccata gaagcctgaa acgatcgctc ccaaatcaag gccgataata tcaccttcct
     3661 tgaggcgacg cttggatgga attccgtgaa caacctgctc gttgaccgat gcacacagcg
     3721 tcttggggta gttccgatat cctttgaagg cgggaatggc accacgaact cgaatcgcct
     3781 cttcagccat acgatccaaa tcatcagtcg taatgcccgg tgccaccttc tcctttaaca
     3841 actccaacac ttctgccacc acatgagaag cctgcgccat taacgagact tcgtccggcg
     3901 tcttgaacac gatcatttag cgccaagccc agcgagcacc ctcgataacc tatcgtaaac
     3961 gtgagtcata tcgccagacc catcaaggtc actcaggact tttttctttt catagtaggc
     4021 aattaacggc gcagtctgct catcataaac tttcaaacgg gcttcaatgg tctccggctt
     4081 gtcatcgctt cgttgaacca gcgaagcgcc acagcggtca cattgaccct caattttcgg
     4141 cgcagcaaaa tctacatgaa acacgctctg gcactttgga caacttcggc gaccactcag
     4201 gcgtctaatc acatcttcgc gagagacccg aaaattaacg acgcgatcaa ggcttacccc
     4261 tcgctcggcc agaattccac tcaactcttc ggcctgggga acagtccgtg ggaatccatc
     4321 taagatgaaa ccattctgac acatcggttc agccactttg gcacgaacca taccgataac
     4381 gacattgtca ggaacaagtt tcccggcatc catactcttt tttgcctcga tccctaaagc
     4441 ggtctggttc ctcacggcct ctctcaagag gtctccggta gaaatcttgg gccaaccaaa
     4501 ctgagcggtc acccgatcag cctgagttcc ttttcccacc cctggtgcac cgagaaacac
     4561 gagacgcatt gaatttcctt acgtcgtccg gccccgaagt ttacccttct gaagaaatcc
     4621 atcgtaattg cgattcagta gatgcgactc gatttgctga gccgtatcaa gcccgacccc
     4681 gatcacgatc agtagagagg tacccccgaa atagaaaggc atattcagtt tgtagataag
     4741 aaactccgga atcacgcaga cgatggcgag ataaatcgcg ccggcaaaag taattttcgt
     4801 caataccgaa taaatatagt cggaggtacg ttgccccggc ctgattccag gaacgaaccc
     4861 accgtatttt ttcaaattat ctgccatatc aacggggttc atcaccaccg cagtataaaa
     4921 gaagcagaag aagacgatca ggccgacata catcaaggta tacagcagcg atcctggggc
     4981 taactgtgag ccaatagcct taatccacgg cgtttcaaaa aacccggcaa tggtggcggg
     5041 aaaggcaata atcgacgaag cgaaaatggg aggaatgact cctgccgtgt tgatcttgag
     5101 gggaatatgt gtactttgcc caccgtaaac ccgtcgacca ataactcttt ttgcatattg
     5161 aactggcact tttcttcgcc cgctctccaa aaagacgatg gcagctacca ccccaaacat
     5221 gatgagcgtc aatacgacca agagcggaaa actcagttgg ccgaccttat acagatcgaa
     5281 cgtttgagca acggcggcag ggagcctggc cacaattccc gcaaaaataa tgagcgaaat
     5341 accgttcccg ataccacgct ctgtaatttg ctcgcccagc cacatcagaa agccggttcc
     5401 ggcgcagagc gtaataacgg tcatcaatcg gaaaccccag cctgggctga gcacgaacgc
     5461 tccttggttc atttgctcaa ggccgacggc aatgccgaat ccctgaatca aagcgatgac
     5521 gatcgtaccg aatcgtgtgt attgaataat tttcttccgg cctcgttcgc cctctttcgc
     5581 taattttgag aggtgaggaa tgaccaccgt caacagctga agaataatcg atgcgctgat
     5641 gtagggcatg atccccagcg caaagattgt cagtcgtgag agggagccac cggaaaaaat
     5701 atccaggaag cctagaagcg caccaccttg cttctgcagg aactcagaaa gagcctctcc
     5761 attgatacca ggcgtgggaa tgtgagcgcc gacacgatag acgataagca tccccagcgt
     5821 aaacaacacg cgggtacgga gctcagggat cttgaagatg ttttgaaaac tagtcagcag
     5881 acgctcaaac acagggaatg acctcggccc tccccccggc tgcctgaatc tttgcctctg
     5941 ctgacttgct gaacttatga gcctgaacca ctagaggctt cgtgagctct ccctgggcaa
     6001 ggatcttaat cagctctccc tttcgtcgca caaggccttc attcatcaag agttggggcg
     6061 taatggcttc tgtcgtctcc agatcagcca gacttttcaa attaatgatc gtatactcag
     6121 ttcgaaattg attcgtgaag ccatacttcg ggacccgacg aatcaagggc atttggccac
     6181 cctcaaaccc agggcgcttc cccccgcctg agcgagcctt aagacccttg tgacccttag
     6241 tcgcggtctt tccatgaccg gaccctggac cgcgcccgat tcgcttccgc ttgtgtttcg
     6301 ctcctcggga aggagcaaga tcatgcagct tcattgggga ctcacttcca gtaaatagcc
     6361 tacctttgca atcatgccct taacctgatc gctggcagga cgcaacacag tggcattgag
     6421 tttccgaagc cccaatcccc tcagcaccaa tcgatgctta tgcggtgtcc cgatagggct
     6481 ccgcttcagg gtgatacgaa ggctcgcctg agcaattgtt gatttctttt cagtagccat
     6541 tagacatgta cctgcgtttc gcttccagca ctgcggcgta gcctcatcat ttcttgagga
     6601 tcacacaact gctgaagccc gtgaagagta gctctgacgg cattgaatgg attgccgcga
     6661 cccaatgttt tagcaatgat gttatgcgct cctgcgagtt cgaccacagc ccgcacagca
     6721 cctccggcga tgattccggt tccatccgca gcaggcttaa gcagcacatg ctcagcacca
     6781 aagagtccgt gtacttcgtg cgggatagta ccacccttga ttggaactcg caccagatgc
     6841 tttttcgctt gctcaacagc ctttgaaatt gcgacgggga cctctgccgc tttgcccttc
     6901 cctacaccaa cgtacccctg cccatctcct accacaacca aagcgcagaa gttaaaacgc
     6961 tttccgccct tgactacttt ggcgacgcgg ttgataaata ccaccttgtc cttgaggctc
     7021 aattcttcgg gattgactcg cacaacgtca ttctccttta agtcgtctca tttggctcgt
     7081 cacgagcaga agatggttaa tctgcacgat tccttgccta aaactgtagg ccgccttcgc
     7141 gcgacgcatc agccaaggcc ttcactcgac catgatactg ccgccctccg cgatcaaaga
     7201 ccaccattga gaccttggca gccttagcac gctcggcgat aagctttcca accgccttcg
     7261 caccctcaat acttccggtt gacttcacgg ttttccgcaa cgactcgtcc aacgtggaag
     7321 ctgccgcaac cgtatgcccg cgaaggtcat cgataatttg ggcgtaaatg tgagcacgac
     7381 tgcgaaacac atttaagcgt ggccgctcac tcgttccgat gactgacttc cgaacacgtt
     7441 gtttacggcg cgctaatttt ctgttttttt cttgggtatt catcagatgc ctctacttgc
     7501 cggtcttgcc cgccttcttc ctcaagactt ccccggcgta tcgaattcct ttttgcttat
     7561 acacatcagg tggcttaatc gagcgaatgt tcgctgcgac ctgaccgact agccgcttat
     7621 caacgccctt aatggagatg agagtttgct tgtcgacctt gacatcaatg cccgcgggaa
     7681 cagtatacgt cacgggatta atgtacccga cattgaaact catctcgcgt ccctgaagct
     7741 gtgccttgta accgacacca gtaatctcaa gcgctttctc ataacccttc gtgacgccct
     7801 ggatgatgtt actgagctcc gcacgaacca acccgtgcat tgcgcgtatc tggcgcgcat
     7861 caccagaacg gttgacaacc agctgcccgt catttacagc aacgctcaat ccatcggtca
     7921 gcttccaatc aagtttccca atcgggccct tgactgaaac aacacgacca gcaaccttaa
     7981 tgtccacacc agctggaact gaaattggtt tccgccctat ccgcgacata cgaaccccgt
     8041 tacgttacag cgaccccagt tattaccgcg cctcagccga cacttttacc agacggaaca
     8101 aagaacttca ccacctaatc cggaccgcct ggaatcactg tcggtcataa gacctttcga
     8161 ggtagagatg accgccacac caatcccatt ccttacctta ggcacgtcat cccgaccgac
     8221 atacactcga cgcccaggct tgctgattcg acgcataccc gtgatcatcg gtcgagcctc
     8281 ttcaacatag cgaagctgga cggagaagta gggatgacca tcttcttgaa attcgccgta
     8341 accaagaa
//
LOCUS       DAORQN010000060         8327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_432_length_8327_cov_832.685687, whole genome shotgun sequence.
ACCESSION   DAORQN010000060 DAORQN010000000
VERSION     DAORQN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_432_length_8327_cov_832.685687"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK614_05240"
     CDS             complement(<1..402)
                     /locus_tag="PK614_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81648.1"
                     /translation="MTAAAMQQVLKWYAKRKIRELCPSSIADQLLEEQGAIHQLWSIG
                     IVTGASPLRLRHASGGLNPVLTREDVSDVRALFVADPFLLRVGDLWHMYFEVYNFDAD
                     RGEIAWATSKDGFAWTYQKVVLRESFHLSYPY"
     gene            complement(399..1328)
                     /locus_tag="PK614_05245"
     CDS             complement(399..1328)
                     /locus_tag="PK614_05245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB81649.1"
                     /translation="MPNLNRWRGERILVTGGSGFLGSHLCQRLLELGAEVHATSRVER
                     TSAPGGPIWWQVDLTAIDRVRKLLTDLKPMVVYHLAGSAGAKPDLGLVLPTLEGLVVS
                     AVNILVAGTEAGCGRIVMTGSLTEPAWGEHAPIPSSPYAAAKWTSSAYARMFHLLYQA
                     PTVVLTPFMTFGPGQDRSKIVPSVILSLLRRESPRLSSCLWEVDWIFIDDLVEAFLAA
                     AAVPDIEGGCFDVGTGTNRTVRSVVERIFLLMGEQTQPLFGMLPDRPSEPVRSADTMQ
                     TYQLLKWRAQISLDEGLRRTIAWYTTHAAMGRA"
     gene            complement(1342..2283)
                     /locus_tag="PK614_05250"
     CDS             complement(1342..2283)
                     /locus_tag="PK614_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRB81650.1"
                     /translation="MNNTIYYDAQLTDDERRKLIFEGQLVAYSPREHSLALVAHARKL
                     IEEAFAPLDPETAQYHLPVEQYAEVLGKLKPHFIHHPDSKKHLRALLAEMGADPEKTY
                     FDVPKMRSSTSDNYLTTGIAYAWHPHRDTWYSAPPCQINWWIPIYDIRSDNAMAFHPK
                     YWNRAVKNDSRSHNYYEWNKQHRGGHVSQFLKADPRPLSKPIETIEIDPQIRIIVPAG
                     GIILFSAAQLHSSVPNTSGKTRFSIDFRVVNVDDAAARRGAPHVDEECTGTTMRDYLR
                     GTDLSQIPADIVALYDDGAQEDGELQYKPKDVSTPSL"
     gene            complement(2442..3782)
                     /locus_tag="PK614_05255"
     CDS             complement(2442..3782)
                     /locus_tag="PK614_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HRB81651.1"
                     /translation="MGEDIEGPGDVKSFDASRVLPDGCGEEMYQFISELYPICRSITG
                     QGIRDTFGRIQQHIPLSITAVPSGTQVFDWTVPLEWNIRDAYVADRQGVRVIDFKQSN
                     LHVVSYSIPTAARMPLRELKAHLFTLPDNPDWIPYRTSYYKESWGFCLSHRKYLDLRD
                     EDYDVVIDSSLKPGHLTYGEYYLPGDTTEEVLISCHACHPSLCNDNLSGIAVATFLAK
                     SLQTGSRRYSYRFLFLPVTIGAITWLALNQQKVSNIKHGFVLTGVGDEGGFTYKKSRD
                     GNADIDAAFAHVLQHSGESFEVTEFIPYGYDERQYGSPGFNLPVGCFMRRANGGYPEY
                     HTSADNLDFVKPTSLSRSLQVVQSVVEVLESNRGYVNMSPYCEPQLGKRGLYRTIGGS
                     VTGQSAEMALLWVLNLSDGAHTLLQIAERAGLPFSTIKLAADALEGQGLLRPAG"
     gene            complement(3823..5058)
                     /locus_tag="PK614_05260"
     CDS             complement(3823..5058)
                     /locus_tag="PK614_05260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB81652.1"
                     /translation="MLRDGCRFCGMLLQHVFLDLGMSPLANSYLKAHQLNQMEPFYPL
                     RAFVCERCLLVQLEEFESPDHIFSDYAYFSSFSDVFLQHAKDYVDMAVERFSLGAQSY
                     VVEIASNDGYLLQNFVARGIRVLGIEPAKNVAEVAVKKGVPSCVKFFGTQTARELVAE
                     ERRPDLIIGNNVLAHVPDLNDFVGGLKVLLAPEGIITIEFPHLMHLMGLNQFDTIYHE
                     HFSYFSLTSVNQVFAKHGLTIFDVDEISTHGGSLRIYARHDEHHSKPVTERLMALKKK
                     EEIQGFATLAHYLSFSEKARETKRALLEFLIRAKREGKSVVAYGAAAKASTLLNYCGV
                     RQDFVDYLVDRSTYKQGHWLPGVHIPIHDPERIKETKPDYVLILAWNLKEEIMKQMSY
                     VRNWGGQFVVPIPEVRICP"
     gene            complement(5124..6485)
                     /locus_tag="PK614_05265"
     CDS             complement(5124..6485)
                     /locus_tag="PK614_05265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRB81653.1"
                     /translation="MKISVMGTGYVGLVSGTCLAERGHQVTCIDIRPEVVQEINAGRP
                     PIHEIGLDNLLRSARDKGMLSATTDAKTAILDSDVTLICVGTPTVDGRMDMSQIVAAA
                     KEIGSALASKRGYHVVAVKSTVLPGTTEGPVKAAIESHSGKKVGDGWGLCMNPEFLRE
                     GRAVEDFRVPDRIVVGATDSMTEEVFLNVYADFTCPKLVTTPRTAEMIKYVSNSLLAT
                     MISFSNEIGNMCSAVPGVDARLVWKGVHLDRRLTPINGQVGGAAGVTEYLWHGLGFGG
                     SCFPKDVAALRSFGRTVGEQTRMLDAVLDINADQPLRMVALLEKELSLAGKTVAVLGL
                     AFKPGTDDLRESPALPLVAELRKKGAKVLVHDPIAMPHAKKRPEFRDVVFAEDWAEAL
                     RKSDACCLVTAWPEYRSIQPTNFQKLMTRALVIDGRGVFEPAAMAAAGVVWRGVGYTP
                     EST"
     gene            complement(6500..7633)
                     /locus_tag="PK614_05270"
     CDS             complement(6500..7633)
                     /locus_tag="PK614_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB81654.1"
                     /translation="MTADTSLFAQAQAWLDEDWARMEKTLGPRLEAFRGKHILVTGAA
                     GFLGFGFLHFFSYLNRNLLKTGRLSIVAADNYIRGCPRWLTELVAASPDIQLLRQDIT
                     KPWTGPAGDRFDFIIHGASIASPKVYRQYPLETLDTNISGLRHMLELAKSHRAESILY
                     FSSSEIYGDPPAHEIPTNEAYRGNVSCIGPRACYDESKRLGETLCYLYHQQHGVRAKI
                     VRPFNNFGPGLRLADGRVLPDFCRDILADRDIVLRSDGSPTRTFCYVSDALTGYLLTL
                     LSSHHAEPFNIGSDSPEISMRELGRMLLEVAGSKRKVIHTPSEEVDYLTDNPNRRCPN
                     LAKSRSLLGYTPLVDLRFGLSRMLQWYKQFVGLEELAKDERVG"
     gene            complement(7719..>8327)
                     /locus_tag="PK614_05275"
     CDS             complement(7719..>8327)
                     /locus_tag="PK614_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB81655.1"
                     /translation="YKGEMIKEYFLNFFAINNDLSVDLSTGKTTIHDGNQPKWTVHLA
                     DTGQTTQTGGRVRRLKKWLENDETFMLTYGDGVADLNISKLLEFHRSHGKIATMTSVR
                     SPARFGRIGFDGDRVTEFFEKPESGEGWINGGFFVLNTKALDYIDGDHTAWEREPVER
                     LAHAGEMVGYKHYGFWSCMDTLKEKSYLEELWNSGKAPWKLW"
BASE COUNT         2058 a         2412 c         1973 g         1884 t
ORIGIN      
        1 gtagggatag gacaggtgaa atgattcccg taatacaact ttctgataag tccaggcaaa
       61 cccatccttg ctggtggccc aggcaatctc accacgatca gcatcaaaat tgtatacctc
      121 gaaatacatg tgccacagat ctccaactcg aagcaggaac ggatccgcga cgaacaacgc
      181 cctcacgtcc gaaacatctt cccgagttag taccggattg agtccccctg acgcgtggcg
      241 aaggcgcaag ggcgatgccc cggtgacaat ccctatcgac cagagttgat gaatagcccc
      301 ctgttcctcc aacaattggt ccgcaatgct cgagggacaa agctcgcgaa tttttcgctt
      361 ggcataccac ttcaggacct gctgcatcgc tgcggcggtc atgcacgacc catagcagca
      421 tgagtagtgt accaagcaat cgtccgcctc aagccctcat ccagggaaat ctgcgcacgc
      481 cacttcaaca attggtaggt ttgcatcgta tcggcgcttc gcaccggttc cgacgggcga
      541 tccggcaaca taccgaatag cggttgcgtc tgctctccca tcagtaagaa aatccgctcg
      601 accaccgacc ggactgtccg gttagttccc gttcccacat caaaacagcc cccctcaatg
      661 tctggaactg ccgcagccgc caggaacgcc tccacgagat catcaataaa aatccaatcc
      721 acctcccaga ggcaggaaga aagccggggg gattctcgcc tgagaagtga aagaatgacg
      781 gacggaacaa ttttgcttcg gtcttggccg ggcccgaatg tcatgaacgg agtaagaacc
      841 acggtcggcg cctgatagag caggtgaaac attcgcgcat aggcgctcga cgtccatttt
      901 gccgccgcat agggagaact tggaatcggt gcgtgctcgc cccaagccgg ttcggtaagt
      961 gatccggtca tcacaatacg gccacagcca gcctctgttc ccgccacaag tatattaaca
     1021 gcgctaacca caagcccttc gagggtaggt aatacgagac caaggtctgg cttcgcgcca
     1081 gcagacccag ccaaatggta caccaccata ggcttcaggt ccgtcagcag tttccgcaca
     1141 cgatcgatgg ccgtaagatc gacctgccac caaatcggcc cacccggcgc cgacgtccgc
     1201 tcaacgcgtg aagtcgcgtg tacctccgcc cccagctcga gcaaccgttg acatagatgt
     1261 gatccgagaa acccactgcc tcccgttacg aggattcttt cgccacgcca ccgattaaga
     1321 tttggcatag aagcaatacg tttacaaaga cggagtggag acatccttcg gcttgtactg
     1381 caactcgccg tcttcctgcg cgccatcatc gtagagcgcg acgatgtcgg cagggatctg
     1441 ggacaagtct gttccgcgca ggtagtctcg catggtcgtt cctgtgcact cttcatcaac
     1501 atgaggtgcc ccccggcggg cggcagcatc atccacattc accacacgga aatcgatact
     1561 gaatcgggtc ttgccagagg tattgggcac actcgaatgc agctgagccg ctgaaaacag
     1621 aataattccg cccgcaggaa caatgatgcg aatttgggga tcgatctcaa tcgtttcaat
     1681 cggcttggaa aggggtcgtg ggtcggcttt cagaaattgg gagacatgtc caccccgatg
     1741 ttgcttgttc cactcgtagt aattgtgact gcgggaatca ttcttgacag cccgattcca
     1801 gtacttggga tgaaaggcca tcgcattgtc cgaacggatg tcgtaaatag gaatccacca
     1861 attgatttga caaggcgggg ctgaatacca ggtatcccga tgcggatgcc acgcatacgc
     1921 tattccggtc gtcagatagt tatcgctggt tgaacttcgc attttgggaa catcaaagta
     1981 agttttctcg ggatcagccc ccatctccgc caacaacgct ctcaggtgtt tcttggaatc
     2041 cgggtgatga ataaagtgtg gcttcagttt gcctagcact tcagcgtatt gctcaaccgg
     2101 caagtgatat tgcgcggtct caggatcgag cggggcaaaa gcctcttcga tcaacttgcg
     2161 tgcatgggcc actaacgcta gggaatgctc tcgaggtgaa tacgctacca gttgcccttc
     2221 aaaaatcagt ttccttctct catcatcagt caattgggca tcatagtaaa ttgtgttatt
     2281 cattcggtac ctcctttggc gttacgcgct tgatgatgcg cgttcgcaac tggaccgcga
     2341 cacggcgctc actcacaaac atccagaccc tccaagaacc atcgcactgc ggctagcatc
     2401 actgagagtt ctgattccca tcttcgcacg ctctcggcac cctaaccagc aggacgtagc
     2461 aatccctgac cttccaacgc atcagcggct agcttgatgg ttgaaaaggg caaccccgct
     2521 cgctcagcta tttgcaataa ggtgtgcgcc ccgtcagaca ggttcaagac ccagagaaga
     2581 gccatttccg cgctctgccc cgtgacgctt cctccgattg tgcgatagag accacgtttg
     2641 cctagctgcg gttcgcaata gggggacatg ttgacgtacc ccctatttga ctccaacacc
     2701 tctacgactg actggaccac ttggagggat cgcgacagtg atgtcggttt gacaaaatca
     2761 agattgtcgg cagacgtgtg atactccgga taccctccat tcgcccgacg catgaaacat
     2821 ccgacgggaa ggttaaatcc cggtgaacca tactggcgct catcgtatcc atatgggata
     2881 aattcagtga cctcaaacga ctctcccgag tgttgtaaga catgggcaaa tgccgcatca
     2941 atgtcggcat tgccatctcg acttttcttg taggtgaagc ccccctcatc acccacgccg
     3001 gtcaggacaa agccatgctt gatgttggac accttctgct ggttgagggc tagccaggtg
     3061 atcgccccga tagtaaccgg aaggaagagg aaacgatatg agtatcgacg tgagccagtc
     3121 tgtaacgatt tagccaagaa cgtcgctacc gcaattcctg ataggttgtc attacacagt
     3181 gatggatggc aggcatgaca tgaaatgagc acctcttctg tagtgtcccc gggcaaatag
     3241 tattcgccat aggtgaggtg tcccggtttc aaggacgagt caatcacgac gtcatagtcc
     3301 tcgtcacgaa ggtccagata tttacgatgg ctcagacaaa atccccagga ctctttgtaa
     3361 taggacgttc tataggggat ccaatccgga ttatccggca gcgtgaagag gtgcgctttg
     3421 agctcgcgta gcggcatacg cgctgcggtc gggatgctat aactcaccac atgcaagttg
     3481 gattgcttaa aatcaataac tcgcaccccc tgacgatcag ccacgtatgc atcccgaata
     3541 ttccactcga gcgggaccgt ccaatcaaac acctgcgttc cgcttggcac ggcagttatg
     3601 ctcaacggaa tatgctgttg aatgcgcccg aacgtgtccc ggataccctg tcctgtaata
     3661 cttcgacaga tcggatagag ttcagagata aattgataca tctcctcgcc gcaaccgtcc
     3721 ggcaaaactc tgcttgcatc aaaactcttc acgtcaccag gcccctcaat atcctcaccc
     3781 accaagattc actcggcaaa agagcttctc tctacacatc gatcaggggc atattcgaac
     3841 ttccggaatc ggcacaacaa actgtccacc ccaatttctg acgtagctca tttgcttcat
     3901 gatttcttct ttgagattcc aggcaagaat caacacatag tcgggcttcg tttccttgat
     3961 gcgttcgggg tcatgaatgg gaatatgcac cccgggtagc caatgtcctt gcttgtacgt
     4021 gcttctatcc acgagatagt cgacaaagtc ctggcgtacc ccgcaataat tgagcagcgt
     4081 actggccttc gccgcggcac catatgcgac tactgatttc ccctcacgtt ttgcccgaat
     4141 caggaactcc aaaagcgctc gtttcgtctc ccgggctttc tcagaaaaag aaagatagtg
     4201 agcgagtgtc gcgaatccct gtatttcttc cttcttcttc agggccatca accgctcggt
     4261 caccggcttt gaatgatgct catcatgacg agcgtagatt ctcaacgaac caccatgcgt
     4321 cgatatttca tcgacatcga aaatggtcag gccatgtttc gcgaagacct ggttcacgga
     4381 agtcagtgaa aagtaggaaa aatgctcatg ataaatcgta tcgaactgat tcaggcccat
     4441 gagatgcatc agatgaggaa actcaatcgt gataatcccc tcaggcgcca agaggacttt
     4501 gagccctccg acgaaatcat tcaggtccgg cacgtgcgcc aagacattat tgccgatgat
     4561 caagtcagga cgcctttcct ccgctactaa ctctcgtgcc gtctgggtcc cgaaaaactt
     4621 cacgcaggac ggaacaccct tcttgacggc cacttcagcc acattcttgg ccggttcgat
     4681 ccccagcacg cgaatgcctc gtgccacaaa attttgcagt agatagccgt cattgctcgc
     4741 gatctccacg acataacttt gggcgcccag cgagaatcgt tccacggcca tatcgacata
     4801 atctttggca tgttggagaa acacgtcgga gaacgaagag aaatatgcgt aatcggagaa
     4861 gatgtggtca gggctctcaa attcttctag ttggactaac agacaccgct cacacacaaa
     4921 tgcccgtaac ggataaaacg gctccatctg gtttagttga tgggccttta aatatgaatt
     4981 ggccaacgga gacatgccca gatcaagaaa gacgtgctgc agaagcatcc cacaaaatcg
     5041 acatccgtca cgaagcatga cctgttctcc tttggctaga acgataacga tacgggcgtg
     5101 cagtcactca agggagtctt tctctaggta gattctggcg tgtatccaac gccgcgccac
     5161 acgacccctg ccgcggccat tgctgcaggc tcgaatacgc cacgaccatc aatcacaaga
     5221 gcacgggtca tcaatttctg aaaattagtc ggctgaatac ttcgatactc tggccacgcc
     5281 gtcacgagac aacaggcgtc gctcttcctc aacgcctcag cccaatcttc agcaaacacg
     5341 acatcccgga attccggtcg cttcttcgca tggggcatgg caatggggtc atggacgagc
     5401 accttcgcgc ctttcttccg aagctcagcc accaagggca aagccggtga ttcccgcaaa
     5461 tcatccgttc caggtttaaa tgcgaggcca agcacggcca cagtcttgcc tgctaggctc
     5521 aactcctttt ccagcagggc aaccattcgg agaggctggt cggcattaat gtccaacacg
     5581 gcgtccaaca tccgagtctg ctcgcccacg gtacgcccaa agcttcgaag cgccgccaca
     5641 tctttcggaa aacaactgcc cccaaacccc aaaccatgcc aaagatactc cgttactcct
     5701 gccgccccac ctacctgtcc attgattggc gtgagccgcc tatcaagatg aacccccttc
     5761 cacaccaaac gtgcatcgac accgggtact gcggaacaca tattgccaat ttcattcgag
     5821 aatgaaatca tcgtggcaag cagggaattt gatacatact taatcatctc agccgtgcgc
     5881 ggggttgtca ccaatttagg acaggtgaaa tcggcataga cgtttagaaa cacctcttcg
     5941 gtcatggagt ctgtggcacc gaccacaatg cggtccggaa cacgaaagtc ctcgactgca
     6001 cgaccttccc tcaaaaactc cggattcatg cacaagcccc agccgtcccc tactttcttg
     6061 cccgagtgag actcaatggc ggccttcacc ggtccctccg tggttccagg caataccgta
     6121 ctcttcactg ccacaacatg gtatccacgt ttactcgcta gagctgaccc gatttccttt
     6181 gcggcagcca caatttgcga catgtccatc cgaccatcca ccgtgggcgt accaacacag
     6241 atcagagtta catcggaatc gagaatggca gtctttgcat ccgtggtggc agacaacatt
     6301 cccttgtcgc gcgccgaacg aagtaaatta tctaatccta tttcatgaat cggtggacgt
     6361 ccggcgttaa tttcctgtac cacctcaggc ctgatatcga tacaggtcac ttggtgacca
     6421 cgctctgcca agcaggtccc tgatacaagc cccacgtatc ctgtccccat cactgatatc
     6481 ttcatggtca ccctaatggt tagccgacac gttcatcttt cgccagctct tccaagccaa
     6541 cgaactgctt gtaccactgc agcattctgc tcagtccgaa ccggagatcg accagcgggg
     6601 tatagccgag aagcgatcga gacttcgcaa gattcgggca gcggcggttg ggattatccg
     6661 tcagatagtc gacttcctca ctcggcgtat ggataacttt ccgcttacta ccggccacct
     6721 ccagcaacat acgtccaagc tcacgcattg aaatttcagg cgaatcacta cctatgttga
     6781 acggttcagc atgatgcgat gataataggg tcaggagata tccggtcagt gcatccgaga
     6841 catagcaaaa cgtgcgtgtc ggactcccat ccgagcgaag gacgatatcg cgatcggcca
     6901 ggatatcgcg acaaaaatca ggcaacacgc ggccgtcggc cagacgcaat ccagggccaa
     6961 aattgttgaa cggtcggacg atcttcgcgc gcacaccatg ttgctgatgg tacaaatagc
     7021 agagcgtctc gcccaaacgc ttcgattcgt cataacacgc ccgcggaccg atacatgaca
     7081 cattgccacg ataggcctcg ttcgttggaa tttcatgagc cggcggatca ccgtaaatct
     7141 cacttgagct gaaatagagg atactttcag cccgatgact cttggcaagc tcgagcatat
     7201 gccgtaagcc ggagatattc gtatccaaag tttccaacgg gtattgtcga tacactttcg
     7261 ggctggctat ggatgcgccg tgaataatga aatcaaaacg atcgcccgct ggtcctgtcc
     7321 agggcttggt aatatcttgt cggagcagct gtatgtcggg gcttgcagcg actaactcag
     7381 tcagccagcg tgggcaaccc cggatgtaat tgtcggcggc gacaatggac agacgcccag
     7441 tctttaagag gttcctattg agatacgaaa aaaaatggag gaatccaaac ccgagaaatc
     7501 ccgctgcccc cgtaacaaga atatgcttcc cgcgaaacgc ctccaaccga gggcccagcg
     7561 tcttctccat tcgcgcccaa tcttcatcca accatgcctg cgcctgtgcg aacaaagatg
     7621 tatcagcggt cattgacatt tgctcctctc tgccggtgca cgatgccgcc aaaagtccgc
     7681 gactttatgc atttcggaat tcctatattc agtgctcgtc accataactt ccagggagcc
     7741 ttgccagaat tccacaactc ctccaggtaa cttttttctt ttagggtatc catgcacgac
     7801 cagaatccgt agtgtttata ccctaccatc tcgccggcgt gagcgaggcg ttcgacaggc
     7861 tcacgctccc aagccgtatg atcaccatca atatagtcca aggcttttgt gttgaggaca
     7921 aaaaatccgc cgttgatcca cccttcccca gactcaggct tctcaaaaaa ctcagtcact
     7981 cggtcgccgt caaacccaat ccgaccaaat ctcgctgggg accggacaga ggtcatggtt
     8041 gcaatttttc catgggaacg atgaaactcc agcaacttgc taatgttcag atctgccacg
     8101 ccgtctccgt acgtcaacat gaatgtttca tcgttttcta gccatttctt caaacgcctt
     8161 acccgtccac ccgtctgcgt tgtctgtcct gtatcggcca gatgaacggt ccacttcggc
     8221 tggtttccat catggattgt cgtttttccc gttgaaagat ccaccgacaa gtcattgttg
     8281 atggcaaaga aattcaggaa gtactctttg atcatttccc ctttgta
//
LOCUS       DAORQN010000061         7980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_451_length_7980_cov_12.380063, whole genome shotgun sequence.
ACCESSION   DAORQN010000061 DAORQN010000000
VERSION     DAORQN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_7980_cov_12.380063"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK614_05280"
     CDS             complement(<1..418)
                     /locus_tag="PK614_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81656.1"
                     /translation="MKVLAFSALVNALAATGLGMFVYFRDPAAARNRIYGLYCLSIAF
                     WSVFYCGWQLTESKDLALSLLRLVMAGASLIPILYFHHTVTFLDITARHAKALKIGYG
                     LAALFLLADTTPWFVAGVHPAMSFSFWPVPGLFFHPF"
     gene            complement(452..3184)
                     /locus_tag="PK614_05285"
     CDS             complement(452..3184)
                     /locus_tag="PK614_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB81657.1"
                     /translation="MSKHIAARNESVSVIGLIGQESSRQSSVSDRKKTSDAWSLSVAE
                     RRASRRLETRISTLLHFQDHTWNGQTKSLSLGGLSMEFAEDIPAMLNQQILLSVTPGA
                     TRPESVGVVCGIRVSESVLGSGQTKRGVTLAVQFARLSPGDEQVVAMWMSEGRARSKG
                     LHLVAALIAQESEDALIDTESRQPVVAQAARARLARVPERPRQERRRQQRITVGLSTE
                     VSLRLRAGDRILSGALTTDLTSNGACLCLSAEEDLLGSELELKWVSPTVAQRGSSVST
                     PSVGCSVVGEVVWTRPGEAGAPSGYRQVGGRTVLAGVRFLPVAKEAEDIIEHLLGQQQ
                     GEGVVDRAEGPSVISEFSECVRPSGHRIALCHDRPRTDLSDDAPIVVLAPGYGESKRD
                     YVPLAYYLAGNGFHVVRFDNVNHVGESDGLVTQFRLEDMETDLETVLDYVAAQWPGRS
                     IGLVATSLAGRVALKVAGRVPHLGLLALINGIMDVQHTLQAVHQEDLIGEHLAGVRKG
                     VVNILGLTIDADRWLEHAVQGGYADLSTTQQDAEHLHAPVVLFHAEQDAWVDPASIEA
                     VGAAMGANLRHSYVVPGALHRLQESPRKARTVYRQIALCCQQELWPTRSMARMVEPSH
                     REIGVQNRAERERSKKRRPVGKSDHVAFWQEYLHNFQTIPNVADFWRLMDHVYRLMGD
                     CHHGERILDAGCGNGNLGVFLQLNQAFRQRYARRGDFRSPDYVGVDFVPTALAQAMTN
                     FEQVGAALQGQFYEGLRAYVPMSMRVCRADLESPLPFPDHSFDRVVCNLVIGYVRDPL
                     FTLREYLRVLAPNGRLVISNLKPYADLSAIYRSFVETAQTPDHIEEGRRLLDNSGRIK
                     EREGEGIFHFHYQAELESLLRAAGVTHPRVYSTFGNQALIAVADKGLANLTVAA"
     gene            3883..4281
                     /locus_tag="PK614_05290"
     CDS             3883..4281
                     /locus_tag="PK614_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81658.1"
                     /translation="MRLPHIRAHSGMLIPVLAGIMLGCHSTPPITEGYPHQRALQGKS
                     KEQVLACAGTPLQEREDGAVTMLRYYKEAPLLEESMVSSKSSRPTVHHGCWATVVIQH
                     QRVDQVQYRFVPSSVDASNDCEEIFANCPE"
     gene            4269..5198
                     /locus_tag="PK614_05295"
     CDS             4269..5198
                     /locus_tag="PK614_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81659.1"
                     /translation="MPGMTARRHRSVSPRWLLFFMGLLSAMMPWTEPALGLEKYGRPL
                     PSMDEPEHEAREEEETLFGGYLLTGAFVKNPTFAARPDNTGLVGMRHMLHLETDLYKQ
                     YLTFYTDQNFFSDRTDGWIKLTEWDGTFAFTGVLDRFNWRLQYERDAPLDRRGLKQAY
                     ADALVTARFQAIQDSTWWRRTFPNQNLTAYAGAGWLFHNSQYFARPNNTGRALFRYVA
                     HADLDVYKNKVVLYGDMNLFTDREASNTLNPSELDWIIGIAVRWKDTELAFYREEDRP
                     LDQSGLVQKYYAVQLRFAFDVSKGALERMGLKR"
     gene            complement(5207..6697)
                     /locus_tag="PK614_05300"
     CDS             complement(5207..6697)
                     /locus_tag="PK614_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRB81660.1"
                     /translation="MLNRIFGLETEYGLLVNQERPDHSPSWVAQRIRDHIFHVERRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYIDMGHLEYASPECTTLADLVASDRAGDRIIQDA
                     VRALGLDETVSLIKNNIDHETDATFGSHENYLVTRQFPFSRRGLGPLVTFLVTRQVFT
                     GAGRIGCASDPNEWVQVGGLILHRPGLRDAQDRSIVPFQISQRADYIVNDFFEWVQHN
                     RAIVNTRDEPLADPNQYRRIHLLCGDSNMAEYATALKMGTTGLVLQLIEAGQAPRGLG
                     IHEPVEALQDISRDPDRRWIVRLESGQSMSAIDIQEQFLAAAQRHCNGQDEETDWVLE
                     QWESVLTDLRGGYEKLVGRIDWASKLWLLETYREAEQCAWDDPMLKSLDLEYHNLHPE
                     RGLCYGLEEEGRGPRLTTDKIVHLAQDHPPRNTRAFGRGELVRHLLASGGGGIVSDAP
                     LDFEEQMACEYIINWSNFKLRGTAPFFMADPFKTYVQDVRTHLAAR"
     gene            complement(6699..7538)
                     /locus_tag="PK614_05305"
     CDS             complement(6699..7538)
                     /locus_tag="PK614_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81661.1"
                     /translation="MYEEPYRWIEAVGNRRQYLDEQFAKGSPVVALAYADGILVLTVS
                     RGTPKLYEIYDRIALGGMGHPADLEKLRFSLLEMAHVEGFNRSPSDVTGARLMKYGLA
                     PTIKQAFEEIYKAPFIAKILLAELGVKPERNLFLSINYDGNFEESQGWAALGATASVQ
                     GRMRRYVESQPAFAQASLAQAVELALCTWAVGSLAQAASESPAAESSSTQLSEEQTEG
                     DGSSLPDKAALLAHVRQTVAEKSLECVLLERRGPGSAKYRALRPEELGRLLPPAVSAP
                     ATT"
     gene            complement(7585..>7980)
                     /locus_tag="PK614_05310"
     CDS             complement(7585..>7980)
                     /locus_tag="PK614_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HRB81662.1"
                     /translation="ALAGTGAVVPVFAGYDHEQESAKIYFYDILGAEFEAVEYAVSGS
                     GSPTIRGILHYVNTWGPQPLATLPEEQATIQALRLLSSAAEFDSATGGVNRELNLYPV
                     VKLIKASGVQTIPDADLKRLHESEVLRGR"
BASE COUNT         1746 a         2532 c         2209 g         1493 t
ORIGIN      
        1 ggaaaggatg gaagaagagg ccgggaaccg gccaaaaaga gaacgacatc gcagggtgca
       61 ctcctgccac gaaccagggc gtcgtatcgg ccagcagaaa cagtgccgcc agaccatagc
      121 cgatcttcag ggctttggcg tggcgcgccg taatgtcaag aaacgtgacg gtgtggtgaa
      181 aatacagaat cgggatgagg gatgcgccgg ccatcaccaa gcgcagcaga ctcagcgcaa
      241 ggtctttgga ctccgtgagt tgccaaccac agtagaagac gctccaaaag gcaatgctca
      301 gacagtacag cccgtaaatc cggttgcgtg cagcggcagg atcccggaag tagacgaaca
      361 ttccgagccc tgtcgcagcc aacgcattca ccagggcgct gaacgcgagc actttcatct
      421 caggtactct gataaaatcg aggccgtgtc atcatgccgc caccgtgagg ttcgccaggc
      481 ccttatcggc cacggcaatg agcgcttgat ttccgaacgt ggaatagacc cgcggatgcg
      541 tgacgccggc ggcacgtaac aaactttcta gttccgcctg gtaatggaaa tgaaaaatgc
      601 cttcgccttc ccgctctttg attctaccag aattgtccag gagacgacgc ccttcttcaa
      661 tgtggtccgg cgtctgagcc gtctcaacga agctgcgata aatcgccgag agatcggcat
      721 acggcttcag attggaaatc accaaccgtc cattgggcgc caacacgcgc agatattccc
      781 ggagggtgaa gagcgggtcg cgcacgtacc cgatcacaag attgcacacg actcggtcga
      841 aggaatgatc gggaaacggc agcggagact ccaggtcggc acggcagacc cgcatcgaca
      901 tcggcacata ggctcgcagc ccctcataaa actgcccttg tagcgccgcc cccacctgct
      961 caaagttggt catggcctgc gccagagccg tcgggacaaa atccaccccg acatagtcag
     1021 gcgagcgaaa atctccgcgc ctggcatagc gctgccgaaa cgcctgattg agctgcaaga
     1081 acacaccgag gttaccattg ccgcacccgg catccaaaat ccgctccccg tgatggcaat
     1141 cgcccatcag gcgatagaca tgatccatga gccgccaaaa atcggccacg ttcgggatcg
     1201 tctggaaatt atgtaagtac tcctgccaga acgccacatg atccgacttg ccaaccggcc
     1261 gacgcttctt gctgcgttcg cgttccgcgc ggttctgcac gccgatttct cgatgcgatg
     1321 gttcgaccat gcgcgccatc gaccgtgtcg gccacaactc ctgctggcag cacagggcaa
     1381 tttggcgata gaccgtgcgg gctttccggg ggctctcttg aagtcgatgc aacgcgcccg
     1441 gcaccacata ggagtggcgg agattcgcac ccatcgccgc gccgactgcc tcgatggagg
     1501 ccggatcgac ccacgcatcc tgctcggcat gaaacagcac gacgggagca tggaggtgct
     1561 cggcgtcctg ctgagtggtg cttaggtcgg catagcctcc ctgcacagca tgctccagcc
     1621 aacgatccgc atcgatggtc aggccgagaa tgttgaccac ccctttgcgc acaccggcca
     1681 gatgttcgcc gatcagatcc tcttgatgga cggcctgcag cgtatgctga acatccatga
     1741 ttccattgat caacgccagc agccccaggt gagggactcg ccccgcgacc ttgagcgcaa
     1801 cgcgaccggc caggctggtc gccacaagcc cgatggatcg acccggccat tgagcagcca
     1861 cgtagtccag cacggtctcc agatcggtct ccatatcctc caggcgaaac tgcgtgacca
     1921 acccatcgct ctctccgaca tgattcacat tgtcgaagcg cacgacatga aacccattgc
     1981 cggcgagata gtaggccaac ggcacatagt cgcgcttgct ttccccatac ccgggagcca
     2041 acaccacaat cggtgcgtcg tccgacagat cggttcgagg ccgatcgtga cagagcgcga
     2101 tacggtgacc ggacgggcgg acacactccg agaactcact gatgacggaa ggcccttccg
     2161 ccctgtcgac gacgccctcg ccctgctgct gcccgagcag gtgctcaatg atgtcctcag
     2221 cctcttttgc aaccgggaga aagcgcactc ccgccaacac cgtccgcccc ccaacctgcc
     2281 gatagccgga aggagcgccg gcctctcccg gtcttgtcca taccacttca cccaccacgg
     2341 agcagccgac agacggcgtg gacacagaag accctcgctg ggcgacagtc ggtgacaccc
     2401 atttcagttc caattctgaa cccagcaaat cctcttcggc cgacagacac agacaggccc
     2461 cgtttgacgt caaatctgtc gtcaacgccc ccgacaagat tcgatcaccg gctctaagcc
     2521 ggagactgac ctcggtgctc aatccgaccg taatccgctg ttgacgcctc cgctcctgac
     2581 gtggacgctc gggtacccgg gcaaggcgcg cgcgcgcggc ctgagcgacg accggctgcc
     2641 gggactcggt gtcgataagg gcatcctcac tctcctgggc gatcaatgcg gcgacgagat
     2701 gcagcccctt cgaccgtgcg cggccctcgc tcatccacat ggctactacc tgctcatcgc
     2761 cagggctcaa ccgcgcaaac tgaacggcga gtgtcacccc tcttttcgtc tgacctgaac
     2821 caaggacact ttcggaaacg cgaatcccgc acacaacccc aacactttcg ggccgcgtgg
     2881 ccccgggagt cacactcagc agaatctgct gattcagcat ggcaggaatg tcctcggcaa
     2941 attccatcga gagcccgcct aagctgaggc ttttcgtctg accgttccac gtatgatcct
     3001 gaaagtgaag gagggtcgag atgcgagtct cgaggcgtcg ggaagcccgc cgctctgcaa
     3061 cagagagaga ccaggcatcg ctggttttct tccggtccga aaccgaagat tgccggctgc
     3121 tctcctggcc aatcaggccg atcacagaca cactctcatt gcgcgcagcg atgtgcttcg
     3181 acatacattc cctcccgccg tattgagtgc tgcgcgttgc acagcatcga attgaataac
     3241 gtgtggacgt cgtcgcccgc tcacagagct cgctccgggc gaggctggac tcttgcaagc
     3301 gcacgaacga ttccaaaaat gcgggaggat gtcggctgat ctaacaaggg caggattaac
     3361 gacacgcacg gcctcagaat tggcacacag cgactgtcat acaacccaat gttcgaagct
     3421 tattctaggg aattagacat aattgtcaac tcatccgcag gaaaaaaatg cagttaccta
     3481 caaaaattaa cttagatgat agctacaggg aagaaagaca ccgttggccg ggtcgccacg
     3541 acgcggaagc agccccagca agtcagaacg atgacgaccg tcatgatcct gacgccgatt
     3601 cctcactttg cgcaaaggcc tgacgcacac cctgcgaggc gctcatcgcc gcatcacagc
     3661 ccgagccatt aaggaatttc acgcggcaga atttgccctc tccacggggg caccggctca
     3721 ccgatgacgg ttaatgcagc ccctcctcag tgaccgacat cgtggcccgg cgcaagctgg
     3781 ttcgacgacc aggcaggcat tggggaatcg gcccgggttg gttgctttga ccaggcaggt
     3841 atgctactca cagtccccga taggtcagcc tgagtactca cgatgcgcct acctcatatt
     3901 cgtgctcaca gcggcatgct gatccccgtc ctggccggaa tcatgctggg ctgccacagc
     3961 accccgccta tcaccgaggg ctatcctcat cagcgggcgc tgcaagggaa aagtaaagaa
     4021 caggtgctgg cctgcgccgg cacacccctc caggaacgag aggatggagc ggttactatg
     4081 ctccggtact acaaagaggc ccccctgctc gaagagtcca tggtgtcctc gaagagcagc
     4141 cgtcctaccg tacatcacgg ctgctgggca acggtcgtga ttcagcacca acgagtcgac
     4201 caggtgcagt atcgcttcgt gccgagttcg gtcgatgcct caaacgactg cgaagagatc
     4261 ttcgccaatt gcccggaatg acggcccggc gacaccgcag tgttagcccg cgctggctgc
     4321 tgttcttcat gggcttgctg tcggcaatga tgccctggac ggaaccggcg ctcgggctgg
     4381 aaaagtacgg gcgcccgctc ccctccatgg atgaaccgga acatgaagcg cgcgaagagg
     4441 aggaaacgct gttcggcggc tatctattaa ccggtgcctt cgtaaagaat ccgacgttcg
     4501 ccgccagacc tgacaatacc ggactcgtcg gtatgcgcca catgctgcac ttggaaacgg
     4561 acctctataa gcaatatctc acgttttaca ccgatcagaa ctttttctcg gaccgcaccg
     4621 acggatggat caagttgacg gaatgggacg gcacctttgc cttcaccggc gtgcttgatc
     4681 gcttcaattg gcggctgcag tacgaacgag atgccccgct ggatcgtcgc ggcctgaaac
     4741 aggcctacgc cgatgcgctc gtgacagccc ggtttcaagc gattcaggac tccacctggt
     4801 ggcggcgcac cttcccgaac cagaatctca cggcctatgc cggcgcagga tggctcttcc
     4861 acaacagcca atacttcgcc cgccccaaca atacgggccg cgcattgttc cggtacgtcg
     4921 cccatgccga cctggacgtc tacaagaaca aggtcgtcct gtacggggat atgaatctct
     4981 tcaccgaccg cgaagcgagc aacaccctga acccttccga actcgattgg atcatcggca
     5041 tcgccgtccg atggaaggat acggaattgg ccttctaccg cgaagaagac cgaccgctgg
     5101 accagtccgg cttggtccaa aagtattacg cagtccaatt gcgctttgcg ttcgatgtgt
     5161 cgaaaggcgc gctggaacgg atgggactga agcggtaggc gggagcctat cgagcggcga
     5221 ggtgggtgcg cacatcctgc acgtaggttt tgaacgggtc tgccataaaa aacggcgccg
     5281 ttccgcgcag tttaaaattc gaccaattga tgatgtactc gcaggccatc tgttcttcga
     5341 agtcgagcgg ggcatccgac acgatccctc cgcctccgct ggccagtaga tgcctgacca
     5401 actcaccgcg tccgaacgcc cgcgtgttgc gaggcggatg gtcctgcgcg agatgaacta
     5461 tcttatcggt cgtcaggcgc ggtcctcgcc cctcctcctc gagaccgtag cagagtcctc
     5521 gctcaggatg cagattgtga tattcgaggt cgagactctt caacatggga tcgtcccacg
     5581 cacactgctc ggcctcccga taggtctcca atagccacag tttcgaggcc caatcgatcc
     5641 ggccgaccag cttttcatac ccgccacgca aatcggtcag cacggattcc cattgctcca
     5701 gcacccaatc cgtctcttcg tcctgcccgt tgcaatggcg ctgggccgcg gccagaaact
     5761 gttcctgaat atcgatggcg gacatcgact gtcccgactc caaacggacg atccaacggc
     5821 gatccgggtc gcgcgaaata tcctgcaggg cctcgaccgg ctcgtggatc cccaggccgc
     5881 gcggggcctg tccggcctca atcagttgca gcaccagccc ggtggtcccc atcttgagcg
     5941 ccgtggcata ttcggccatg ttcgaatccc cgcacaggag atgaatccgg cggtattggt
     6001 tcggatcggc cagcggctcg tcacgtgtat tgacgatcgc acggttgtgt tgaacccact
     6061 cgaaaaagtc gttgacgatg tagtcggcgc gttgggagat ctgaaacggc acaatcgatc
     6121 gatcttgcgc atcccgcagc ccaggccgat gcaggatgag ccccccgact tgcacccact
     6181 cgttgggatc actcgcgcat ccgatgcgcc ccgccccggt gaacacctga cgggtgacca
     6241 aaaacgtcac cagtggaccg aggccgcgcc gggaaaaggg aaactgccgc gtgacgagat
     6301 agttttcatg cgagccgaac gtcgcatcgg tctcgtgatc gatattgttc ttgatgagcg
     6361 acaccgtctc gtccaacccc agcgccctca ccgcatcttg aataatccga tcccctgcac
     6421 gatccgaggc cacaagatcc gccagggtcg tgcactccgg agaggcatat tccagatgcc
     6481 ccatatcgat gtagatacgg cccgcattgg tcagaaaccc cccgttgccc ggcggctcgt
     6541 catgaccacg atggtgaaga tcgaggactc ctcgccgttc gacatgaaaa atgtggtcgc
     6601 gaatacgctg cgccacccag gagggcgaat ggtcgggacg ctcctggttc accaggaggc
     6661 cgtattccgt ttcaagtccg aagatacgat tcaacatatc aggtggtggc cggtgcgctg
     6721 acagccggag gcaacagacg gcccagttcc tcaggacgaa gcgcgcgata tttggccgat
     6781 ccaggcccgc ggcgctccag caggacacat tccaatgact tttcggcaac ggtctgacgc
     6841 acatgtgcca gcaacgctgc cttgtcaggg agactcgaac cgtccccttc cgtctgttct
     6901 tccgatagct gggttgaaga tgactccgca gccggggact cggacgccgc ttgggctaac
     6961 gaaccgacgg cccaggtgca cagggcgagt tccacggcct gcgccagcga ggcctgcgca
     7021 aacgccggtt gcgattcaac atagcgtcgc atccggccct gcacggaagc cgtcgcgccc
     7081 aatgccgccc agccctggct ctcctcgaaa tttccgtcgt agttgatgga cagaaacagg
     7141 tttcgctcgg gtttcacgcc caattcggcg agcaaaattt tggcgatgaa gggggcttta
     7201 taaatttctt caaaggcctg cttgatggtc ggtgccagcc catacttcat gagcctggcg
     7261 cccgtgacat cggacggcga tcgattgaaa ccctcgacat gggccatctc caggagggaa
     7321 aagcgcagct tctccagatc cgccgggtgc cccattccgc ccagcgcaat gcgatcgtaa
     7381 atctcgtaga gtttgggcgt tccgcggctg acggtcagca ccagtatccc gtcggcatag
     7441 gccaacgcga cgaccggtga gccctttgca aattgttcat cgagatactg gcgtcgattg
     7501 ccgaccgcct cgatccaccg atagggttct tcatacataa cgtgccacct tcaccatagg
     7561 ggtcaataac cagccgtcta tctgttagcg tccgcgcagc acttcgctct catgcaatcg
     7621 tttcaagtcg gcgtccggaa tggtctgcac accggacgcc ttaatgagtt tcaccaccgg
     7681 atagagattc agctcccgat tcaccccgcc ggtggccgaa tcgaattccg ccgcgctgga
     7741 gagcaaccgg agcgcctgga tcgtggcctg ttcctccggg agggttgcca atggttgcgg
     7801 gccccatgtg ttcacataat gtaggatgcc tcgaatcgtc ggtgagccag aaccggacac
     7861 ggcatactcc acggcttcaa actctgcacc cagaatatcg taaaaataaa tcttggcaga
     7921 ctcctgctcg tggtcatatc cggcaaagac cggaaccacg gcaccggtcc ctgccaacgc
//
LOCUS       DAORQN010000062         7890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_456_length_7890_cov_19.730823, whole genome shotgun sequence.
ACCESSION   DAORQN010000062 DAORQN010000000
VERSION     DAORQN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_456_length_7890_cov_19.730823"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PK614_05315"
     CDS             complement(<1..784)
                     /locus_tag="PK614_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB81663.1"
                     /translation="MDSSFTLTIDWLAFTVLASNPQETMRVLGGGWSKAKGGFRGYPL
                     SWMRVDGLRGVGKLGTNAPRCPNEIHVDLSGGLASALTLDQIRTLLKWVHAQQGHVTR
                     IDCALDDRAGSVPVATIREAVSAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELQSKGHENYQDYGTRWELELKKDRAEQCARALASLDEADWKELVV
                     GLLRSYVDFRQITKETEDEDRYRAPVLEWYALLTEGFQKGRLA"
     gene            1592..2944
                     /locus_tag="PK614_05320"
     CDS             1592..2944
                     /locus_tag="PK614_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81664.1"
                     /translation="MSNGASCGYAFVLGVPLFLSTAKLLTVAFVLVSSSWGFASTITV
                     DLAAEEGVIYHRANGYLVSIAPTQPGQDLIVPLKPTSFRGSVRHVLQNYDRLKEAGVH
                     EFQLTVGYTFHDLGMQAFDVNRIGQDGDYTPWLAHVDHLIDQVRARHISVDWDIYNEP
                     DLATLPIAENKALLEGWRLAYKRIKDRIPEAIVVGPSVSRYRLLAPFLEWCQAHDVFP
                     DVVSYHEYGDPSEAVGYVNDLHAYLKTRGLARPISVNEIMGQESWTSAGYTAGVIAAY
                     EKADILSAMRACWPDPQDSSSSYIENTCDNPTLDGLLYVDGQSKRPGWHVYKTYADLQ
                     GTRINTTTDSPRIHALAALDKASGALRVLMGKHADYSMENTQLVLKHLNRMRAQEERD
                     VLHICAEQIPDVGSAYLGSTVQTLDSDLPHDEDDLSLTLPLFNHSDAYRVTLSFLRQC
                     "
     gene            complement(3081..4424)
                     /locus_tag="PK614_05325"
     CDS             complement(3081..4424)
                     /locus_tag="PK614_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81665.1"
                     /translation="MKPATSKARSFLWYQTSAVLILNTIICFVLFNALLAMAFWGIDS
                     YASGRMAKKIKTMIGLHQETARLFNPDGSPVDNGSRNFYQLAWVDFHAYGGLSPSYVA
                     AVLDESYRLAERGMVYQPWVEFAEPEFHGSLVTVQNDERNFLNRRTINPPNLEHWPIV
                     KIFALGGSTTFGYNVSDEHTWPTYLSTILNEQARAKSLKLHIEVVNYGRGYYYPSQET
                     SLLLDLLKAGHRPQGVIFLDGVNSPVKDDVPEFYPRLKEQFQNIQFAKDTQRGTSFIE
                     SIQWLPAIRLANAVGSLLSGRQPLTVAQRHSAVDFNGVRYQVNTFRQNKAISKEICRI
                     YAVTPMFVLQPSAVYNYNIALYRRSVPPEFLTWRARAKDFYDAMREDEDLIDLADLFE
                     NWGNDKKAIIDELHYSPQFHQFLAQQIAKRIDLTKLDPSRAPLDLSAATGSRTEN"
     gene            complement(4795..5921)
                     /locus_tag="PK614_05330"
     CDS             complement(join(4795..5666,5666..5921))
                     /locus_tag="PK614_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB81666.1"
                     /translation="MKRTRRNHGAAFKAQVAVAAINGDKTLAELAEQFSVHPTQITEW
                     KQQLLARAADVFAGNKPMADTPDLKVLHAKIGQLALENDFLGRGAHQGRLAERKAMID
                     RTHPLPVRQQCQLLNLARSTAYYHPTPVSETVLVLMRRIDELHLAHPFAGARMLRDLL
                     RRDGHAIGRRHVATLMRRMGLEALYRKPRTSHRHPAHTIYPYLLRELTIRRPNHVWAA
                     DITYIPLARGFVYLFAVLDWASRRVLAWRLSNTLTTDFSLAAVQEALTQYGTPEIFNT
                     DQGCQFTSQEFTGLLTHHGIQVSMDGKGCWRDNVFVERLWKSIKYEEVYLHAYETIAS
                     AHQGLAQYLTFYNQTRPHQALDSQTPDQVYSDHLTTRLTAA"
     gene            6126..6455
                     /locus_tag="PK614_05335"
     CDS             6126..6455
                     /locus_tag="PK614_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81667.1"
                     /translation="MRGTVATIILGLVLTTGCSTTNQVATQDQNYNAPLSIYSAMDST
                     SLVYSDPMAASQANDHPYRWAAFILHPVGHLVDYTVNRPLYAIAKHAPYLFGYTSEDS
                     MIDSQRR"
     gene            6606..7751
                     /locus_tag="PK614_05340"
     CDS             6606..7751
                     /locus_tag="PK614_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81668.1"
                     /translation="MYRQLSLLPFLFAACALWAGCETPPPARPPLPPSESDLDLLGPT
                     KLCDSRQTFLAAHPGIIPHTHAWGSGQELRIPSEHSRSKSEESYFFDEDGTLVGMLFV
                     FRSGLDLAPYKTLRYTLSRLKPSLEFYLTVAQLADRQNMEASTIYDTGDEKTTTRYLV
                     LGDRSDQRLLEASFTVDPYVKLFSPYRKEFLDRLRNTAQQTGGQHLDTQGSEDKEPFA
                     SLQQFARGQTAQLAYCGTKNQVIALDAYQKASASGFSGTVWQAELRHRLGVSWEAAGN
                     LEKAKSELLASLALRPNSPEVVNNLGAVYWKLGEKKNALASFEKAILMRPNYAIARFN
                     VAEAIADDNPRRAITEYETYLALVEGILEETDRAALAQKRVQALKHQ"
BASE COUNT         1799 a         2199 c         2091 g         1801 t
ORIGIN      
        1 gggccaatcg ccccttctga aatccctccg tcaggagggc ataccactcc aacactggag
       61 cccggtaacg gtcttcgtct tctgtctctt tcgtgatctg tcggaaatcc acatacgagc
      121 gaagcaagcc gaccaccaac tccttccaat cggcttcatc tagtgaagcc agcgcacgag
      181 cgcattgttc ggcgcgatcc ttcttgagtt ccaattccca ccgagtcccg tagtcctggt
      241 agttctcatg gcctttgctt tgcaattcta agcgcttgtc atagatccgc aacaaggtct
      301 gactctgagg gctcccgaag tacatcgtct cgcccgtcgt cgctccggtg ccgtgcgtga
      361 ggttggagac gatatgccgg acctgagccg cccgcgtcac acattggcct gccgagaccg
      421 cttctcgaat tgtcgccact ggtaccgatc cggctcgatc atccagggca cagtcgatgc
      481 gtgtcacgtg gccttgttgg gcatgcaccc acttgagcag ggtgcggatt tgatccaatg
      541 tcagggcgga cgccaggccg cccgacagat ccacatggat ttcattcggg caacgagggg
      601 cattcgtgcc tagtttgcca accccgcgca ggccgtctac ccgcatccag gacaagggat
      661 accctcggaa gccgcctttg gccttgctcc agcctccgcc caatacgcgc atggtctcct
      721 gggggttgct cgccaggacc gtgaaggcca accaatcaat cgtgagggtg aaactcgaat
      781 ccatggactc tatcgaactc ctgtacggag cgctgtgggt agcgcccccg tgttgttacc
      841 aagacggggg ccattccgct ccgcgcccca gcctgccggc atgcggcgcg gaccggcttc
      901 gcctccgatc caaggacgga cgtgttgccc cgaacagggc tccaatcgtg atgtcatggt
      961 ttgctcttgg ccattccttc cgttttcaga ccctatgtga aagacgtgta ttaagtaaag
     1021 tacccacgag aggccacgaa gggacagtag gggacatggg aggacaaatt atcgggacgg
     1081 tgggaccaaa agggaagtag gcatatgtat gcttaagggt gttttagtgc actggtggag
     1141 gcaaaaacga gtttgatctt gtggccgcga accaattcaa gaaaaatccg gaggctctga
     1201 caatgctgca agaaacattt taaatgaata gctgcatcgt gccgtcaatt cattgcaacc
     1261 caggctaagg aaatgataat ttcacgggac tacgacttca aattatggct cggtcaaccg
     1321 gtgatgaatc aacgagttga tgatcactat aggttgacta agtgagtcaa attctcatcc
     1381 aacacgatcc actccgggca gttcgtaaag cgctgacgat aaggacatcg gaagtatagt
     1441 agcggtgtta cataggtttt acggggctca agatctggtt gtacgcccct cgatatttat
     1501 ttttccgtgg tgaatgcgct cgcttagggg atagcgcgtt ataaaaacga tgcttcaact
     1561 gacaaaagcc gaccatcgac taacgaggac tgtgtcaaac ggggcttcat gcggttacgc
     1621 atttgtattg ggcgtccccc ttttcctttc taccgccaag ctgctgacgg tggcattcgt
     1681 gttggtttcg tcatcgtggg gttttgccag cacaataacg gttgatctag cagcagaaga
     1741 aggcgtgatt tatcatcgag ctaacggcta cctcgtaagt attgcaccca cacagccagg
     1801 tcaggactta atcgttccgc ttaagccgac tagttttcga ggaagcgtca ggcacgtcct
     1861 tcagaactat gatcgcttaa aggaagcggg agtccacgag tttcagctca cggtggggta
     1921 cacctttcac gacctgggca tgcaggcatt cgacgttaac cggataggac aggacgggga
     1981 ttacacgccc tggcttgctc atgtagacca ccttattgat caggtgaggg ctcgccacat
     2041 ttctgttgac tgggacatct acaatgagcc ggatcttgca acattgccaa ttgcagagaa
     2101 caaagctctg cttgaaggtt ggcgattagc ttataagcgc attaaagacc ggattcctga
     2161 ggccatagtt gttgggccca gtgtgagccg gtatcgattg ttggcgccat tcttagaatg
     2221 gtgtcaggcg catgacgtat ttccggatgt cgtgagctat cacgaatacg gagatccttc
     2281 agaagccgtc gggtatgtaa atgacttgca tgcctatctc aaaactcgcg gtcttgcaag
     2341 gcccatttcg gtgaatgaga tcatggggca agagtcgtgg acgtcggctg gatacactgc
     2401 cggtgtgata gcggcttacg agaaggcaga cattttgagt gccatgcgtg cgtgctggcc
     2461 agacccccag gactcgagca gtagttacat agagaatact tgcgataatc ctaccttgga
     2521 cggtctgctg tatgtagacg gccaatccaa gcgacccgga tggcacgtct ataagacgta
     2581 cgctgacctg cagggtacaa ggataaatac cactaccgac agtccgagaa ttcacgctct
     2641 cgctgcgctg gacaaagcct ctggcgcact aagggtgttg atggggaagc atgcagacta
     2701 ttcgatggaa aacacccagt tggtgcttaa gcatctgaat cggatgagag ctcaggagga
     2761 aagagacgtc ctgcacatct gcgcggagca aatccccgat gtcgggtcgg catatcttgg
     2821 atccacagtg cagacactag acagcgactt gcctcacgat gaggacgacc tctccctcac
     2881 ccttccacta ttcaaccact cggatgctta tcgagtcact ttgtcttttt tgaggcaatg
     2941 ttagctttca agaacaggcc agtagcatct tcctgagtgg taccaaccac ctcgccttga
     3001 gagatttcgt aggttccaac caatttggct aggcaggaca agtctattgt gttgatgtct
     3061 gcaccttggt gtcagaccaa tcagttttca gttctcgatc cggtcgcagc cgagagatca
     3121 agcggcgctc ggctggggtc aagcttagtg aggtcgattc gcttcgcgat ctgttgtgcg
     3181 agaaactgat gaaattgcgg actgtagtgc aattcatcaa taattgcctt tttatcgttg
     3241 ccccagtttt caaacaggtc ggccaaatca atgaggtcct cgtcctctcg cattgcgtca
     3301 taaaagtctt tagcgcgagc gcgccatgtc agaaattcag gcggcactga ccttcgatat
     3361 agcgcgatat tgtaattata cacagcagac ggttgcaaca cgaacatcgg tgtcacagca
     3421 taaatccggc aaatctcttt tgagatcgct ttattctggc ggaaggtatt tacctggtac
     3481 cgcacaccat taaaatccac ggcactgtgc cgttgagcaa ccgtaagtgg ttgtcgccca
     3541 gaaaggagag accccacagc atttgcaaga cgtatcgctg gtagccactg aatggattct
     3601 ataaaagagg tcccccgctg ggtgtcttta gcgaactgga tgttctgaaa ctgttcttta
     3661 agcctggggt aaaattctgg gacatcatct ttgacggggg agtttacgcc atccaagaaa
     3721 atgacaccct gtggtcgatg gccagctttc aggagatcga gaagaaggct agtctcctgg
     3781 ctgggataat aatatcccct gccgtagtta acgacctcaa tgtgaagctt taaggatttg
     3841 gctcgcgcct gctcatttaa gatagtggaa agataggttg gccaagtgtg ctcatccgag
     3901 acattgtagc caaatgtcgt gctaccaccc aaggcaaata tcttgacaat gggccagtgc
     3961 tccaaatttg gaggattaat cgtgcggcgg tttaaaaagt ttcgctcatc attttgcact
     4021 gtaactaagc ttccgtgaaa ctctggctcg gcaaattcga cccacggttg gtacaccatc
     4081 ccacgctctg ccagtctata tgactcgtca aggacagcag cgacatacga aggactaagc
     4141 cctccatagg cgtgaaaatc cacccaagct aattggtaaa agttgcgact gccattgtct
     4201 acgggtgaac cgtccgggtt gaacaacctt gccgtctcct ggtgaagtcc tatcatcgtc
     4261 tttatttttt ttgccatgcg tccagaagca taggagtcta taccccaaaa cgccatggcc
     4321 aagagagcat taaaaagtac aaagcatata atggtgttta aaatgaggac ggcagaagtt
     4381 tgataccaaa ggaagctgcg cgctttggag gtggctggct tcatttgaca cctaggatta
     4441 atattttatc cttaggtctc gttttcattt ttcctccagc ccacctgcag gctccccgtt
     4501 taatctcgct aatgtcagaa ttgcggcaca atccgtttac tctacgagcc cccactactg
     4561 gctactgtaa gaggaaaaag tgcataaata cttttcctct cttacccaat cgaaaaggcg
     4621 tgtgctaaat ttgtgctaaa agtcgagtga aaagttacta ttggctcgaa ttactagaat
     4681 caatggaatc ctgtcaacca tttaattcac aataagaaac ggggagacca gaggtggctc
     4741 cggttggttg gacagcctag gccattccgt aagtggtgcc tggcgggtcg ttgattacgc
     4801 ggccgtgagc cgtgtcgtca gatggtcaga gtacacctgg tcaggcgtct ggctgtcaag
     4861 tgcctgatgc ggccgggttt ggttatagaa cgtcagatac tgcgccaagc cctggtgcgc
     4921 cgacgcaata gtctcgtagg cgtgcagata cacctcctcg tacttgatac ttttccagag
     4981 tcgttccacg aagacgttgt cccgccagca ccctttgccg tccatgctga cctggatgcc
     5041 gtggtgcgtc agaagtcccg tgaactcctg actggtgaat tggcagccct gatcggtatt
     5101 gaagatctcg ggcgtgccat actgggtgag tgcctcctgc accgccgcca gagagaaatc
     5161 cgtggtcagt gtgttggaca gccgccacgc caacacccga cgactcgccc agtcgagcac
     5221 ggcgaacaga tagacgaagc cccgcgccag cggaatgtag gtgatatccg ccgcccagac
     5281 gtgattgggg cgacggattg tcagttccct gaggagatag ggatagatgg tgtgggctgg
     5341 atgccgatgg ctcgtgcggg gcttgcgata cagggcctcc aatcccatgc ggcgcatgag
     5401 ggtggccacg tgccgacgcc caatcgcgtg gccgtctcgc cggagcagat cgcgcagcat
     5461 ccgagccccg gcaaacggat gggccagatg tagctcatcg atccgtcgca tcagtacgag
     5521 caccgtctct gataccggcg tcgggtggta gtaggcggtc gagcgggcca ggttcaacag
     5581 ctggcattgc tgccggaccg gcagtggatg ggtgcgatcg atcatggctt tgcgctccgc
     5641 aagcctgcct tggtgagcgc cccggctaaa aaatcattct ccagggccag ttgtccgatc
     5701 ttcgcgtgga ggaccttgag atccggggtg tccgccatcg gcttgtttcc agcaaacacg
     5761 tcagccgccc gcgccagcaa ttgctgcttc cattcggtga tctgcgtggg atggacactg
     5821 aattgctcgg ccaactcggc cagcgtcttg tcgccgttga tggccgcgac agctacttgc
     5881 gccttgaagg cggctccatg attccgtctc gttctcttca ttgcctcgct cctctcgtag
     5941 gccacctcgc ggtggcgttg gtgaagccag gctaccactt atcacagtgt ccgaatttcc
     6001 ggagccctct ctgccagctg cccgcgccct caactggaca gaatcaagcg cctgagctag
     6061 acttttctca caaggaacag acttcgtttt tacgaatgtt ccgacagaag aaagagaggc
     6121 atggtatgcg cgggactgta gcaacgatca tattgggact ggttctgacc acgggctgct
     6181 cgaccaccaa tcaggtggcg acacaagacc agaactataa tgcgccgctc agtatttata
     6241 gtgcgatgga cagcacctca ctggtctatt ccgatcccat ggccgcctca caagccaacg
     6301 accaccctta tcgatgggct gcgttcatcc tccatccagt cggccacctc gttgactaca
     6361 ccgtaaaccg ccccctctat gccattgcca aacacgcacc ctacctcttc ggttatacat
     6421 ccgaagattc catgatcgac tcgcagcgac ggtaaccctg ctcgagacct tgccggccgg
     6481 gacctctccc ggcggcgagg tgagcgttcc ttgcccgcct attgccagtc gcatctccct
     6541 tgccgttcag gtatgctcgc cacctcgatc gtgcgccacg ttgaaccggg gcatcgaatc
     6601 cggccatgta tcgtcaactc tcactgctgc cgttcttatt cgctgcctgc gccctctggg
     6661 caggatgcga aactccgcca ccggctcggc ctccgcttcc tccctcagag agtgatcttg
     6721 acctcctggg acccacaaaa ctctgcgatt cccgccagac attcctggca gcacatcctg
     6781 gcatcatccc ccacactcac gcctggggca gcgggcaaga actccgaatc ccgtctgaac
     6841 acagccgctc caagagcgag gaatcgtatt ttttcgacga agacggaacc ttggtcggca
     6901 tgctgttcgt gtttcgttcc ggactcgatc tggcccccta caagacgctg cgatataccc
     6961 tctcccggct gaagcccagc ctggagttct atttgaccgt ggcgcagttg gccgatcgcc
     7021 agaacatgga agccagtacc atctatgaca ccggcgacga aaaaacgacc acccgatatc
     7081 tcgtgctggg cgatcgcagc gaccaacgcc tgctcgaagc ctcctttacg gtcgatccct
     7141 acgtgaagct cttttctccc tatcgcaagg agtttcttga ccgcctccgc aataccgctc
     7201 aacaaaccgg cggccagcac ctcgatacgc aggggtctga ggacaaggaa cccttcgcat
     7261 cgctgcaaca gttcgcccgt ggccaaaccg cgcaacttgc ctattgcggc acaaaaaatc
     7321 aggtcatcgc cctcgacgcc taccagaaag ccagcgcctc gggattcagc ggcacggtct
     7381 ggcaagcgga gcttcgccac cggttgggcg tctcctggga agcggcgggc aacttggaaa
     7441 aggctaaaag cgaactcctc gcctccttag ccctacgccc caattccccg gaagtcgtca
     7501 acaaccttgg tgccgtgtac tggaaactgg gcgagaagaa aaatgccctg gcctccttcg
     7561 aaaaagccat attgatgcgc cctaactacg ccattgcccg cttcaacgtg gccgaggcca
     7621 tcgcagatga caacccgcga cgggccatta cggaatacga aacctacctg gctctcgtcg
     7681 aaggcattct cgaagaaacg gaccgtgccg cgctcgcaca aaaacgcgtg caagcactca
     7741 agcaccagta ggcctatttg gattgcagag tgaacactcg cccggcatgg agccgaacat
     7801 gagaaacatg gggacagatc agcgggggga cttttcggct tcttcctgac tggtcttaca
     7861 ctcaatgcat aacgtggtca cagggcgggc
//
LOCUS       DAORQN010000063         7834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_461_length_7834_cov_45.618589, whole genome shotgun sequence.
ACCESSION   DAORQN010000063 DAORQN010000000
VERSION     DAORQN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_7834_cov_45.618589"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PK614_05345"
     CDS             complement(<1..750)
                     /locus_tag="PK614_05345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81669.1"
                     /translation="MAYHVPFIDPRSHYAKLKPEIDRAIIECLANGDLVNRHQLKDFE
                     QHLAEFVGVKYAVGVNSGYHALLFALLGAGVGPGHEVITVAHTFVATVSAIVHCGAQP
                     VLIDVGPDFNMDIDLIERAITPRTKALLPVHLNGRVCEMDKILALADKHGLAVIEDAA
                     QALGATFDGNCAGSQGRAGCFSFYPFKVLGGFGDGGAITTNDPDLARMATLLRYNGED
                     RATGEYHYHGQTALLDNVQAAVLDVKLRHLPD"
     gene            complement(767..1741)
                     /locus_tag="PK614_05350"
     CDS             complement(767..1741)
                     /locus_tag="PK614_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB81670.1"
                     /translation="MRTLTYAQAIREAHAQLLAEDPRVFLIGQGVRNPWYAGTSLQDL
                     DKEFGRDRIIDSPVSENATTGAAIGAAITGMRPIVFHPRMDFMLLAADPIMNQAANWS
                     YLFAGQVSVPVVIRAVINRGGEQGAQHSQAVQALFAHVPGLKVVMPSTPYDAKGLLIA
                     AVRDGNPVLYIDDRWLYELEGEVPEGLYEAPIGSAAIRRSGRDVTVVATSYMAAEAVK
                     AAATLAQRGIDVELIDLRSIKPWDRNLVYSSVGKTGRLVIADAAWMSGGIAAEIAASV
                     AGEIFHALKAPIARVCLPDAPAPTSAVLEQAYYVGAEDIVTSVEKVLV"
     gene            complement(1746..2750)
                     /locus_tag="PK614_05355"
     CDS             complement(1746..2750)
                     /locus_tag="PK614_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRB81671.1"
                     /translation="MKLQNSDGTSLAREQLTSFLYRMQRIRMAEERIGQLVARREVRT
                     PCHLSIGQEAIPVGVCAALNENDTVWGGHRSHGHYLAKGGDLNAMMAEIFGKATGCAR
                     GRGGSMHLVAPAQGIFGTVPLVAATIPLAVGAALSAKLRGTDQVAVAFFGDGATDEGH
                     FHESLNLAALYRLPVLFVCENNFYSTHLTLKERRIKDNIVESAALHGLAGVVVDGNDV
                     TAVYRAAHEAVECARSGEGPTLLECRTYRWRGHVGPAADLEVGADRRRELDEWQRRDP
                     IAQCRALLGAGGVPFDVLDGVTAKVREEIELAVMFARQSPDPDDSGLLQHVYAARRGC
                     "
     gene            complement(2747..3397)
                     /locus_tag="PK614_05360"
     CDS             complement(2747..3397)
                     /locus_tag="PK614_05360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB81672.1"
                     /translation="MSNAGLLMKRCFDFLLALLGLMLMWPALLAFGLFIKAEDHGSVF
                     YRGPRVGLGGRPFRILKFRTMRPDAERSGVTSTANDDPRVTRIGSVLRRYKLDELPQL
                     WNVLCGEMSLVGPRPEVQRFVDLYTEEEKAILTVRPGLTDWASLWNIDEGATLEGSAD
                     PDQAYCELIRPKKLQLQLAYVRQAGFWTDLGILCQTMGAVLFRVKFCEARALELGS"
     gene            complement(3558..4706)
                     /locus_tag="PK614_05365"
     CDS             complement(3558..4706)
                     /locus_tag="PK614_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81673.1"
                     /translation="MRIALITNDDFSMWHFWRGLIRALKARSVDVIVVTPDGPFVVAL
                     LGIGARHRAIRLKRFMSPWSDIRFCFRLYRLFKAEHVDLVCNITVKPNVYGAIVGKLA
                     GVQRIVGMVEGLGYGFNEGWAWQGKARVGVVKMLYWLAGRLSDRMGFANPDDYATFLS
                     LGLISKRKAVVFRSMIGVNTQEFCPSSRENACDHNTGESRPLSIVMVVARVVSSKGVR
                     EFVQASQIAKGWPRKVEFTLVGPLDPDAEDAIDESYLRQMLSETFRWVGFTNDIRSVW
                     DSADIAVLPSYYREGVPRSLLEAMAMGKPIVTTDNVGCREVVEEEQNGFLVQPRDSAG
                     LAAAIEKLVSDDERRRLFGERSRQKAVAEFDEVAIIKRIMEELYHIET"
     gene            complement(4739..5926)
                     /locus_tag="PK614_05370"
     CDS             complement(4739..5926)
                     /locus_tag="PK614_05370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81674.1"
                     /translation="MLAVTIFVEQRYLRTPNGSIWGPQLGYSFWRNYLSVFDCVRVVA
                     RVLDVSHVSDQWVRADGDSVSFECIPYYVGPWEYLAKRRTVKQALLAALGQQDALIMR
                     LDSQLAAVAYPYLRQHHRPYGVEVVTDPYDVFAPGSGHVLRPVFRLKFAWQLKQQCAG
                     ACAAAYVTRAALQERYPPSRGAFSTWYSSVELPEERFTVAPRTRFGSEGSFRIITVGS
                     MEDFRKGQDTLLDAVARCVSSGLDVRLSIVGSGRTQRSLETRAGDLGLKLLVNFTGQL
                     SPERVVAELDQSDLFVLASRGEGLPRSLLEAMARGLPAIGTRVGGFPEVLPASDLVRP
                     GDVVVLANKIRDTLLNVGRMRDMSARNLTKAREYHQEVLCDRRIEFYRAVRQQTEAWL
                     VKV"
     gene            complement(6025..7323)
                     /locus_tag="PK614_05375"
     CDS             complement(6025..7323)
                     /locus_tag="PK614_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HRB81675.1"
                     /translation="MADAAMRWSVMLIGIVISGMLITLSTELPHWILGLVLLTGMSMV
                     VVRFDLLHPFTWFSPFFFLYSASVPILVWLEIREDIGSLHETIFLEWIALAVFMLVVG
                     PQQRAPRFEGTISVDARITAWLLSTISIAVSGLYLWHIWQANLTSKYEIALSTSIFAK
                     LDPAFSVFAIAYAVLVAGAFQRQRFPWALILFAVGWNALSFLLSGERDLVFRIFWVTI
                     FLYHALYREIPRWVLAILATSAIVLVPIMEDLKNLLLKETETSVIMSSMAVRVLNDEF
                     LTASENLQYYLSRAAFEPFFLGETLLWDIKRALLPWFMAQEGPDPQRYFNIMFFPDIV
                     ERGGGKGFTLVGEGYMNFGWLGVVLWFAFLGWFVRALYVKALASKMWFLMYVVSMPLI
                     VYIIRADFSNLLAQFVKHIAFPMLVIVLGQEILRRRQPKA"
     gene            complement(7389..>7834)
                     /locus_tag="PK614_05380"
     CDS             complement(7389..>7834)
                     /locus_tag="PK614_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81676.1"
                     /translation="DSYWRLMGKLMLVAFLIGLAGVVVATLGGSELLRIVYKEDFGRF
                     SHILVLIMVLGAAESICSVLGAGLTAARRLAAQLPVLAGTLIITTVSGWWLIPSHAMD
                     GAVLAAMAGMVAWGAAYFWILRIPRDVSSRGQDGSSSVHCEHALK"
BASE COUNT         1902 a         2445 c         1975 g         1512 t
ORIGIN      
        1 gtctggcaga tgacgcagct tcacgtccag aacagcagcc tgcacgttgt ccaacaaggc
       61 agtctgccca tggtagtgat actcgcccgt ggcgcggtct tcaccgttat accgaagcaa
      121 ggtggccatg cgcgcaaggt ccggatcgtt cgtcgtgatg gctccgccat cgccaaaccc
      181 accaaggacc ttgaagggat agaaactgaa gcagccggcg cgcccctggc tccctgcaca
      241 attcccgtca aacgtggccc cgagagcctg ggctgcatcc tcgatcaccg ccagcccgtg
      301 cttgtcagcc agcgccaaaa tcttgtccat ctcgcacact cgcccgttga gatgcaccgg
      361 cagcagggcc ttcgtccgcg gcgtgatcgc gcgctcaatg agatcgatat ccatattaaa
      421 gtcaggcccc acatcgatca ggaccggctg cgccccgcaa tgcacgatgg cggagaccgt
      481 cgcgacaaac gtatgcgcca cggtaatcac ttcatggccg ggcccgacac cggctccgag
      541 gagcgcaaac agtagcgcat ggtagccgct attgacgccg acggcgtact ttacgcccac
      601 gaattcggcc aggtgctgct caaaatcctt gagttgatgg cgattcacca gatcgccatt
      661 ggccaggcac tcaataattg ctcgatcgat ctcgggcttg agcttcgcgt agtgactacg
      721 ggggtcgatg aaagggacgt gataggccat aaggaagagg ggcaccctac acaagcactt
      781 tttccacgct ggtcacaatg tcctccgcgc cgacatagta agcctgctca aggacggcgg
      841 aagtgggagc gggcgcatcc ggcaggcaga cacgagcgat cggcgccttc agggcgtgaa
      901 agatctctcc ggccaccgag gcggcgattt ctgccgcgat cccgccgctc atccaggccg
      961 cgtcggcaat gaccaatcgg ccggttttcc ccaccgaaga atacacgaga ttgcgatccc
     1021 agggtttgat cgaacgcaga tcgatcaact ctacatcgat gcctcgctga gccagcgttg
     1081 cggccgcctt gaccgcttca gccgccatgt aggacgtggc caccaccgtc acatctctcc
     1141 cgctgcgacg aatcgcggca gagccgatcg gcgcttcata caggccttcc ggcacctccc
     1201 cttccagttc atagagccag cggtcgtcga tatagagcac cggatttccg tcacgcaccg
     1261 cagcgatgag cagccccttg gcatcatacg gggtactcgg catcaccacc ttcagccccg
     1321 gcacatgcgc aaacagcgct tgcacagcct gggaatgttg agccccttgc tcaccgcctc
     1381 gattgatgac cgcgcggatc accaccggca cagacacctg accggcaaag agataagacc
     1441 aattggcggc ctggttcatg atgggatcgg ccgccagcaa cataaagtcc atgcgcggat
     1501 gaaagacgat cggacgcatc ccggtaatgg ccgccccgat cgccgcgccg gtcgtggcat
     1561 tctcggacac aggggaatca ataatgcgat ctctgccgaa ctccttatcg agatcctgca
     1621 aactcgttcc cgcataccag ggattccgga caccctgtcc gatgagaaac acgcgcggat
     1681 cctcggcaag gagttgtgca tgagcctcac ggatcgcttg agcataagtc aatgtgcgca
     1741 tgcgcctagc atcccctccg tgcggcatac acatgctgca gcaatccgct gtcatctggg
     1801 tctggagatt ggcgcgcaaa catcaccgcc agctcgatct cttccctcac ctttgcagtc
     1861 acgccatcca acacatcgaa gggcactccg ccggcaccga gtaacgcccg gcattgtgca
     1921 atgggatcac ggcgctgcca ctcatccaat tcacgccgcc gatcggctcc gacctcgagg
     1981 tctgccgctg gtcccacatg cccacgccat cggtaggtcc gacactccag cagcgtgggg
     2041 ccttcgccgg atcgagcaca ttccacggcc tcatgcgcgg cccgatagac agccgtcacg
     2101 tcgttcccat ccacgaccac acccgccaac ccatgaagag ccgcactctc gacgatattg
     2161 tcctttatcc gtcgttcttt cagcgtcaga tgagtggagt agaagttgtt ttcgcagaca
     2221 aacagaaccg ggagacgata cagcgccgcc agattgagcg attcgtgaaa gtgtccctcg
     2281 tctgttgcgc cgtctccgaa aaacgccacg gcgacttgat cagtccctcg taacttggcg
     2341 gagagggcag cgcccaccgc caaaggaata gtggcggcca ccaagggtac cgtcccgaag
     2401 ataccctgcg ccggagccac aaggtgcatc gacccaccac gcccgcgagc gcagccagtc
     2461 gcctttccga aaatctccgc catcatggcg ttgagatctc cgcccttcgc caggtaatgc
     2521 ccatgcgagc gatgcccgcc ccagacggta tcgttctcgt tcaatgccgc gcagacaccg
     2581 acgggaatag cttcctgtcc gatcgagaga tgacagggag tccgtacctc ccgcctcgcg
     2641 acgagttgcc cgatgcgctc ctccgccatg cgaatccgct gcattcgata aaggaacgac
     2701 gtcaattgtt cgcgtgctaa cgaggtccca tcactattct gaagtttcat gaccccagct
     2761 ccagtgcccg tgcctcgcaa aatttcacac ggaacagaac cgcccccatg gtctgacaca
     2821 gaataccgag atcggtccag aaaccagcct gacgcacgta ggcgagttgg agttgaagtt
     2881 ttttgggcct gatgagttcg caataggcct gatcgggatc ggcactccct tcgagggtag
     2941 ccccttcatc aatgttccat aaactggccc agtcggtcag acctggtcga acggtgagaa
     3001 tcgctttttc ttcctcggta taaagatcca caaatcgttg cacctccggt cgcggtccga
     3061 ccagactcat ctcgccacac aacacattcc agagttgcgg tagttcatcc agcttgtaac
     3121 gtcgcaggac tgacccgatg cgggtgacac gcggatcgtc gttggctgtt gaagtaacgc
     3181 cggaccgttc ggcatccggg cgcatcgtgc ggaatttgag aattcggaag ggtcttccgc
     3241 ccaagccgac acgcggccca cggtaaaaga ccgagccgtg atcctcggcc ttaatgaata
     3301 gcccaaacgc gagtaacgca ggccacatca gcatcagccc aagcagtgcc aacagaaaat
     3361 caaaacatcg cttcatcaat aggcctgcat tgctcatctc taaagaacat tcccccatcc
     3421 cgccgaatct aacctatggg ccggaggcgc gttgcactgt ctcaaaatcc gtttagtatg
     3481 cacgccaagg ctgctcggct tcaagtgggg ccttaggcga gcagagcggc aggttcttgc
     3541 tcggccgtga cctaagttca tgtctcaatg tgatagagct cctccatgat cctcttgatg
     3601 atcgccacct catcaaactc agccacggcc ttctgacgag atcgttctcc gaatagtctt
     3661 cttcgctcat cgtccgaaac caatttctca atcgcagccg caagcccggc cgagtctcgt
     3721 ggctgaacca ggaagccatt ttgctcctcc tccacgacct ctctacaccc gacattgtcc
     3781 gttgtcacta tcggcttacc catcgccata gcttccaaga gactgcgcgg caccccttcc
     3841 cgataatagg aaggcaatac tgcaatatca gcggaatccc atacgctacg aatatcgttg
     3901 gtaaatccca cccaccggaa cgtctcggac aacatttgcc tgagataact ttcgtcgatc
     3961 gcatcctcgg catcggggtc gagggggcca acgagcgtga actccacttt cctgggccag
     4021 ccctttgcaa tctgactggc ttgcacaaac tcgcgcacgc ctttactcga gaccacacga
     4081 gccacaacca tgacgatgga aaggggcctg ctctcaccgg tattgtgatc gcaagcattc
     4141 tccctgctgc tggggcagaa ctcttgggta ttcaccccga tcatgctccg aaatacgact
     4201 gccttccgtt tcgaaatgag gcccagtgag aggaaagtgg cataatcatc tggattggca
     4261 aatcccattc gatctgataa gcgaccagcc aaccaataga gcatcttgac gacaccgact
     4321 ctcgctttcc cctgccatgc ccaaccttcg ttaaagccgt accccaaccc ttcgaccatg
     4381 cccactattc tctgcacccc cgccaatttc cccacgatgg caccatatac attgggtttc
     4441 acggtgatgt tgcacacaag gtcgacgtgc tccgccttga aaaggcgata caggcggaaa
     4501 caaaaccgaa tgtcactcca gggactcatg aaccgcttca atctgatggc ccgatgtcgc
     4561 gccccaattc caagaagcgc aacaacaaag ggcccgtccg gggtcacgac gatcacgtcc
     4621 accgaccgag ctttcagagc gcgaatgagt cctctccaga aatgccacat ggagaaatca
     4681 tcattcgtaa ttaacgcaat gcgcatatgt gcagtgcgga cagctcagtg aggctatcct
     4741 agaccttcac cagccaggct tctgtctgct gcctgaccgc acgatagaat tcaatccgcc
     4801 ggtcgcaaag gacttcctga tggtattctc gagccttcgt gagatttcgt gccgacatat
     4861 ctctcattcg gccaacattc agcaatgtgt cccggatctt gtttgcgagg acgacgacat
     4921 ctcccggccg gaccaagtca gatgcgggca acacctcagg aaatccacca acgcgggtgc
     4981 caattgccgg caaaccacgc gccattgcct ccaataacga tcgaggcaac ccctcccccc
     5041 gcgaggccaa aacaaagaga tcagactggt cgagttcggc aacgacccgt tccggggaca
     5101 attgcccggt gaaattcacc agtaatttca gacctaaatc tcccgctctg gtttccaaag
     5161 atcgctgagt acggccgcta ccgactatgg aaagccggac atcaagcccg ctcgacacac
     5221 agcgtgccac agcatccagc agtgtatcct gacctttcct aaaatcctcc atgctgccaa
     5281 cagtgatgat tctaaaggaa ccctcgcttc caaaccttgt tcggggagct acagtgaacc
     5341 tttcttccgg caattcgacg cttgaatacc aagtggagaa ggcgccgcgg gacgggggat
     5401 atcgttcttg gagcgccgcg cgggttacat aggctgcggc acatgctcca gcacactgct
     5461 gctttaactg ccatgcgaat ttcaaccgaa agactggacg taagacgtgt ccggaacccg
     5521 gcgcaaacac atcataagga tccgtcacga cctcaacccc gtacggcctg tgatgctgcc
     5581 tcagatacgg atacgccacc gcggcgagct gggaatccag cctcataatc agggcatctt
     5641 gttgccccag cgcggccaaa agcgcctgct tgaccgtgcg tctctttgca agatattccc
     5701 agggtccgac gtagtagggg atgcactcaa aggagacgct gtccccgtcg gcacgcaccc
     5761 actgatccga gacgtgtgag acatcaagca cgcgcgcaac cacgcgcacg caatcgaaaa
     5821 ctgacaggta gttacgccag aaggaatacc ccagctgagg gccccagata gacccgttag
     5881 gagttctcag gtaacgttgc tcaacaaaaa tagttacagc gagcattcac acgccaaaag
     5941 agtcacgcga gcgagcctat cgtagaaggc cgcacctacg tcgctaaccc agactgccac
     6001 ggctgtaaac aagggtgata cggatcacgc cttaggctgt cttctgcgca aaatctcttg
     6061 tcccagcaca ataaccagca tggggaaagc gatgtgtttc acgaattgag cgaggaggtt
     6121 cgaaaaatcc gctcgtatga tgtacacgat gagcggcata ctgacaacgt acataagaaa
     6181 ccacatcttg cttgccaatg ccttgacgta taaggcccgg acaaaccacc ccaggaatgc
     6241 aaaccacaat acaacgccga gccagccaaa gttcatgtac ccttccccta ccagcgtaaa
     6301 cccttttccc ccaccacgct ccacgatgtc aggaaagaac atgatgttga aataccgctg
     6361 cggatcaggc ccctcttgcg ccatgaacca tggaaggagg gcccgcttga tatcccatag
     6421 tagcgtttcg cccaggaaga acggctcaaa cgctgctcga ctcaggtagt attgcagatt
     6481 ctcagaagcc gtgagaaatt cgtcgttcaa tacgcgaacg gccatagaac tcatgatcac
     6541 tgaagtttcc gtttctttca gcagcaggtt cttaaggtct tccataatcg gcaccagcac
     6601 gatagcgctg gttgcaagaa tcgcgaggac ccatctcgga atctcccgat acagcgcatg
     6661 atagagaaag atcgtgaccc agaagattcg aaacactaga tctcgttccc cgctgagaag
     6721 gaacgacagt gcattccatc caacggcaaa caagataagc gcccagggga atcgctgtcg
     6781 ctgaaaagcg ccggcaacga gtacggcata cgcgatggca aaaacagaga acgcagggtc
     6841 cagcttcgcg aagatggagg tggacaaggc gatctcatac ttcgacgtga gattggcctg
     6901 ccaaatgtgc cagagataaa ggccggacac agctattgaa atggttgaca atagccatgc
     6961 agtgattctc gcatctacgc tgatcgtccc ttcgaagcga ggcgccctct gttgcggacc
     7021 aacgacaagc atgaacaccg cgagcgcaat ccattccaga aatatcgttt cgtgcaagct
     7081 tccgatgtct tccctgattt cgagccaaac taagatcggc acggatgcgg agtagaggaa
     7141 gaagaacgga ctgaaccagg tgaagggatg aaggagatca aatctcacta ccaccatgga
     7201 cattccggtc agcagtacca gccccagtat ccaatgggga agctctgtgc tcaacgtgat
     7261 aagcatgccg gagatgacga tgccgatgag catcacgctc cagcgcatgg ctgcatcagc
     7321 cataggcact aggcacaaac caaggaaaga tacaggtaag cgttgtcttc ctgccgcagc
     7381 gtgtgaagtc atttcaaagc gtgctcacaa tggacactgg agctaccatc ctgacctcgt
     7441 gatgagacat ccctgggaat tctaaggatc caaaagtacg ctgcccccca tgctaccatg
     7501 ccagccatcg ccgctagcac cgccccatcc atggcgtgac tgggtatcag ccaccaaccg
     7561 ctcactgtgg tgatgatcaa tgtccctgcg agaaccggca gctgcgctgc caaccttctg
     7621 gcagcagtca gtccggcgcc cagaaccgaa cagattgatt cagcagcacc gaggaccatg
     7681 atcaggacga gaatatgaga gaaacggcca aagtcttcct tgtagacaat acgtaagagc
     7741 tctgaacctc ccagtgtggc gacgactaca cccgccagtc ctattaagaa tgccacgagc
     7801 atcaacttac ccatcaaccg ccaatagcta tcca
//
LOCUS       DAORQN010000064         7734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_463_length_7734_cov_35.885662, whole genome shotgun sequence.
ACCESSION   DAORQN010000064 DAORQN010000000
VERSION     DAORQN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_7734_cov_35.885662"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PK614_05385"
     CDS             <1..748
                     /locus_tag="PK614_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81677.1"
                     /translation="PVSVTMAMQADSFFGFNPSIYGTYGLTDNIALAFNFTYWTMING
                     VGVHDNAPWLETDMGLNFTFLEKRLSVTPMIGFVHGQLLSSRGGFFPNGGGSVNERTT
                     AFEGVVPNIIANYTDDRFEGEFYLGYYKAIRSEGGSGGGGATGCTGLVDTNCLGASQT
                     GGRGTWDFLHYWVSAGMRVNSMWSLGAHYEHLLTTRDSSAPGAAQDYYRWIGPYVEMK
                     LPGNMAFRFTVGKDLTDNQDFYKLKFTKTF"
     gene            823..2154
                     /locus_tag="PK614_05390"
     CDS             823..2154
                     /locus_tag="PK614_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRB81678.1"
                     /translation="MVKRSAQRKTVVVGVAGALILLAESWGTQVWAQNTPLKVDTGDT
                     AWVLVSSAFVLAMLMPGLALFYGGLVRTKNVLGTIMQSVMILSVVSLLWILFGYSLAF
                     GPDKGGVIGGLEWVGLSGVGSEPHPVYGPTIPHQAFMLFQLMFAAIAPALITGAFAER
                     KRFTSVVLFAALWSVFVYVPLAHWIWGGGWLAKLGALDFAGGAVIHISSGAAALVCAI
                     VLGKRRGYGTDYMAPHNLPMTLLGTGFLWFGWFGFNGGSALGANGIAVSAIIATHAAA
                     AMGAIAWCGAEWAHRGKPTVLGVASGAVAGLATVTPAAGYIAPMSAIAIGLVAGMTCY
                     AAIVWKGRFGYDDSLDVVGIHGVGGVIGILATGLFASKVVNPGGADGLFFGNPGLFGI
                     QLLVAAVTTIFSIIGTFVILKLVDSMTGLRVSSEEEATGLDLSQHNERAYS"
     gene            2262..2600
                     /locus_tag="PK614_05395"
     CDS             2262..2600
                     /locus_tag="PK614_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRB81679.1"
                     /translation="MKLIEAIVKPFKLDEVKDALLEIGIQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVNDSQVQRVLETITRAAKTGSIGDGKIFVRELTSAVRI
                     RTGETGESAL"
     gene            2603..5287
                     /gene="glnD"
                     /locus_tag="PK614_05400"
     CDS             2603..5287
                     /gene="glnD"
                     /locus_tag="PK614_05400"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRB81680.1"
                     /translation="MSHAVNADSHTAQDAAPASVSQLLVEQRQAIRQRLLAGASGDEV
                     VTATTDMVDGLIVGRYRTAARTGGEALTTAGFQHCCLVAIGGYGRRELAPYSDIDLMF
                     LFHPDAAKVMPELVKQVLHPLWDSGFQVGHSVRTIQDCVDLGLADLTVRTSMMEARFL
                     AGSADLFQQFHARYVRKVVAHGFDTYLDQKVAERQREYQKFGETVYLLEPNVKKSKGG
                     LRDLHLLQWIGAARFHTATIRELSDRGILSQADYRAIKEAKEFLLRVRSFLHTRAGMA
                     QEILTFDEQVWLATHLGFTDRPHLLAVEQFMQQYYRHTMGLHAALMRFVERCRRRSLW
                     QRVVRWLPSPRIDQYFVVDGEALTVPVELRSQVLARPASLLTLFDLARSRRLNIDPSL
                     LEDIHRQAETLSAEQFHTPETGRTFLSILAGPGTARTLEAMHRAHLLEKLVPAFSRVR
                     GLMQFNQYHKYTVDEHSLLAVAKAEALAEHTGVLGEVYREIKRKDLLHLALLLHDLGK
                     GHEEDHSEVGKRLAEETAARLGFDERDSRTLVMLVHRHLLMAHTAFRRDPYDEKVLLP
                     FAREVGTPEVLRKLLALTAADIAAVGPDVLTKWKESLLIELYLRAMQEVSGERETADE
                     PQRLARIADDVASQQGADRDLLSDKAWIRSELEQFPLRYVYGTSPRRIAAHLGAIRRL
                     QVGGVVVETEFNAPLGTCEYAVIAHDDLIPGLFSKIAGVMAAGGLQILDAQIVTRKDG
                     VVVDTFQVADPDYQGAPPADRCESIAGTITEVLTGRQAIETLMRRGTRLNMGRSLPAH
                     RQPSEVRIDNETSDRFTILDVFADDRQGLLYIITNAIFQLGLSVHASRISTRLDQVAD
                     VFYVTGMDGKKVEEAGRLESIRASILNEIELFLGAHAA"
     gene            5338..6747
                     /gene="glnA"
                     /locus_tag="PK614_05405"
     CDS             5338..6747
                     /gene="glnA"
                     /locus_tag="PK614_05405"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRB81681.1"
                     /translation="MNVREVLDFAKKNKVQVVDMKFVDLIGTWQHFTIPVSELTEGLF
                     KDGSGLDGSSIRGWRAINNSDMLLVPDPATACMDPFCSVPTLSLIGNVVDPITREMYD
                     RDPRFIAQKAEKYLQSTKIGDTSYWGPEAEFFIFDHARYDQTNHSAYYHIDSDEGVWN
                     MGQEGVNLGGKIRHKEGYFPVPPTDTQQDIRTEMILEMEKAGIATEKHHHEVATAGQA
                     EIDIRFDSLLRTADKMMMFKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQSIWKD
                     GKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAFTNPSTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPAANPYLAFAAMLMAGLDGIENKINPGEPA
                     EKDLYDLEAKEAAKIRTMPGSLDEALNHLEKDHQFLLKGGVFSEDLIEAWIGYKRAKE
                     VDTMRLRPHPYEFFLYYDV"
     gene            7062..>7734
                     /locus_tag="PK614_05410"
     CDS             7062..>7734
                     /locus_tag="PK614_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRB81682.1"
                     /translation="MHTADHERIAGVAAKKWSFFERSPGGYLILSALAGIYLGFGIAL
                     IFSVGGPLAAAGSPVVKLVMGVSFGIALTLVIFAGSELFTGNNLIGTIGGLTRSLTWA
                     QVMQLNAWSWLGNLAGSLGLAWLIVQSGVFAKGPTTELIEKVASVKMSLPAWELFVRG
                     ILCNWLICLAVWMTGRTNNDTAKILLIFWCLFAFIGTGFEHSIANQSLLGMALFLPHD
                     AAVSWG"
BASE COUNT         1503 a         2060 c         2542 g         1629 t
ORIGIN      
        1 ccccgtgtcg gtgaccatgg cgatgcaagc cgacagtttc ttcgggttta atccctccat
       61 ctacgggacc tatggactga ccgacaacat cgccctggcc ttcaacttca cctattggac
      121 catgatcaac ggcgtgggtg tgcacgacaa cgcgccctgg cttgaaactg atatgggctt
      181 gaattttacc ttcctggaaa agcggttatc ggtcactcct atgattgggt tcgtgcacgg
      241 gcaattgctc tcttctcgcg gcggcttctt tcccaacggt ggtgggagtg tgaacgaacg
      301 aacgacggcg tttgaaggcg tcgttcccaa cattatcgcg aattatacgg acgatcgctt
      361 cgaaggcgag ttctatctgg gctactacaa agccatccgg agtgaagggg gcagcggagg
      421 cggcggtgcg accggttgca ccggactcgt cgacacgaat tgcttgggcg ccagtcagac
      481 cggtggccga gggacctggg acttcttgca ttattgggtg agtgccggaa tgcgcgtgaa
      541 cagcatgtgg tccctcgggg cccactacga gcatttgttg acgacgagag acagcagtgc
      601 ccccggggcg gctcaggact actatcgctg gatcggaccg tacgtcgaaa tgaaattgcc
      661 ggggaacatg gccttccggt tcacagtggg taaggacctg acagacaatc aggatttcta
      721 taagttaaag ttcacgaaga cgttttagtc tctcgcccgg gcctcctcct ctgctgagtc
      781 agggaggagg ccgatcggtg agcggcgcag ccgaaggagg atatggtgaa gagatcggca
      841 cagcggaaaa cggtcgtcgt gggcgtggca ggggcgttga tcctgctggc ggagtcatgg
      901 gggactcagg tctgggcgca gaatacgccg ctgaaggtcg ataccggtga tacggcatgg
      961 gtgctggtgt cctcggcttt tgtgctggcg atgctgatgc cgggactggc gctgttttac
     1021 gggggcctgg tacggaccaa gaacgtactc ggcacgatca tgcaaagtgt catgatcctg
     1081 agcgtggtga gtctgctgtg gatcctgttc gggtatagcc tggcgttcgg gccggataag
     1141 ggtggggtga tcggtggctt ggaatgggtc gggctcagcg gggtgggcag tgagccgcac
     1201 ccggtctatg gaccgacgat tccccaccag gccttcatgt tgttccaatt gatgtttgcc
     1261 gccatcgcac cggcgttgat caccggtgcg tttgccgaga ggaagcggtt tacgtcggtc
     1321 gtcttgtttg cggccttgtg gtcggtgttc gtctatgtgc ccctggcgca ctggatctgg
     1381 ggcggaggct ggctggccaa gctgggtgcc ttggatttcg ccggcggcgc ggtgatccat
     1441 atcagctccg gggcggcggc cctggtctgc gccattgtgc tggggaagcg gcgagggtac
     1501 gggacggatt atatggcacc ccacaatctg ccgatgacat tgctcggaac cggattcctc
     1561 tggttcggct ggtttgggtt caatggaggc agcgcactcg gcgccaacgg gatcgccgtc
     1621 tccgcgatta tcgcgaccca tgcagcggcg gcgatggggg ccattgcctg gtgcggcgcc
     1681 gaatgggcgc atcgcggcaa acccacggtg ctcggagtcg ccagcggcgc ggtggcagga
     1741 ttggcgaccg tgaccccggc ggccggctat atcgcaccca tgtcggccat tgccatcggt
     1801 ttggtcgccg gcatgacgtg ctacgccgcg attgtctgga aagggcgatt cggctatgat
     1861 gactcgcttg acgtcgtggg aatccacgga gtcggggggg taatcgggat tctggcgacg
     1921 ggactgttcg ccagcaaggt cgtcaatccg ggcggcgccg atggattgtt cttcggtaac
     1981 cccggcctct tcggcatcca actcctggtc gcggccgtca cgacgatttt ctcgatcatc
     2041 ggcacgtttg tcattctcaa gctggtggat tctatgaccg ggctacgggt gtcgtctgag
     2101 gaagaagcga ccggtttgga cttgagccaa cataacgagc gggcgtattc gtgaagccag
     2161 ctcagcgcaa tcggccatca gtcgtcagca aaattgtcag gactctttcc tcgcctgaga
     2221 gctgactgct gacggctgag agctactccc tggaggcaag gatgaaatta atcgaagcca
     2281 tcgtcaaacc attcaagctc gacgaggtga aggacgccct gctggaaatc ggcattcagg
     2341 gcatgacggt cacggaggtt aagggcttcg gccgccagaa gggccataaa gaaacgtatc
     2401 gcggccagga atacaccatc gagttcgtgc cgaaggtgaa gatcgaggtc gccgtgaatg
     2461 acagccaggt gcagcgtgtg ctcgaaacga ttacacgtgc cgccaaaacc ggcagcatcg
     2521 gcgacgggaa aatctttgtg cgggaactca cctccgccgt caggattcgt acgggcgaga
     2581 cgggggaaag cgccctctag gtatgtcgca cgctgtgaat gcggattctc atacagccca
     2641 ggacgcggcc cccgcgtcgg tatcccagct gctggtcgag caacgacagg cgatccggca
     2701 acggctgctc gcgggcgcct cgggcgacga ggtggtcacg gcgaccaccg acatggtcga
     2761 tggattgatc gtcggccgct accggactgc ggcgagaacc ggcggcgagg cgctcacgac
     2821 ggctggcttt caacattgtt gcctggtggc gatcggcggg tatggccggc gggaattggc
     2881 gccgtactct gatatcgacc tgatgtttct gtttcacccg gacgccgcga aggtgatgcc
     2941 cgagctggtc aagcaggtgc tccacccgtt gtgggacagc ggattccagg tcggccatag
     3001 cgtacggact attcaagact gtgtggattt gggcctcgcc gatctgacgg tccggacgtc
     3061 gatgatggag gcccgtttcc tggccggcag cgccgacctg ttccagcagt ttcatgcgcg
     3121 gtatgtccgg aaagtcgtgg cgcacggatt cgatacctac ctcgaccaga aggtggccga
     3181 gcggcagcgc gagtaccaga agttcggtga aacagtctac cttctggaac cgaacgtcaa
     3241 aaaaagcaaa ggcgggttgc gggatctcca tctcctgcag tggatcgggg ctgcgcgctt
     3301 ccataccgcg acgattcgtg aattgtccga tcgcgggatt ctctcgcaag ccgattatcg
     3361 ggcgatcaag gaggcgaaag aatttttgct gcgcgtccgc tcgttcctcc acactcgggc
     3421 cggcatggcg caggaaattc tcacgttcga tgagcaggtc tggctggcca cacatctggg
     3481 cttcaccgac cggccgcatc tgctcgccgt cgaacagttt atgcagcaat actatcggca
     3541 cacgatgggg ctgcacgcgg cgttgatgcg atttgtcgag cggtgtcgcc gtcgctcctt
     3601 gtggcagcgc gtggtgcgat ggttgccgtc tccccggatc gatcagtact ttgtcgtcga
     3661 cggcgaggcg ctgaccgtgc cggtcgagtt gcggtcccag gtcttggcac gccccgcttc
     3721 gctgctgacg ctgttcgatc tggcgcgatc gcgccgattg aatatcgatc catcgctcct
     3781 ggaagacatt catcgtcagg cggagacgct gtcggctgag caatttcata caccggagac
     3841 cggccgtacg ttcctgtcga ttctcgcggg gcccggcacg gcgcggacac tggaggcgat
     3901 gcaccgtgcg catctcctgg agaaactggt gccggccttt tcgcgcgtgc gcgggctgat
     3961 gcaattcaat cagtaccaca agtacacggt cgacgagcat agtctgttgg cagtggcgaa
     4021 ggccgaagcc ttggctgagc ataccggtgt gctcggtgaa gtctatcggg agatcaaacg
     4081 gaaggatttg ctgcatctgg cgttgctgct tcacgatctg ggaaagggcc acgaagaaga
     4141 ccacagcgaa gtcggtaagc ggttggcgga agaaacggcc gcgcgattgg ggtttgatga
     4201 gcgggactcg cggacgttgg tcatgttggt gcatcgccat ttgctgatgg cccatacggc
     4261 ctttcgtcgt gatccctacg atgaaaaagt gttgctgccg ttcgcgcgcg aagtgggcac
     4321 gccggaagtg ttgcgcaagt tgctggccct gacggctgca gatatcgctg cagtcgggcc
     4381 ggatgtcctg accaagtgga aagagtccct gttgatcgag ttatacctgc gggccatgca
     4441 ggaagtgtcc ggcgagcgtg agacggcgga cgaaccgcag cgcctcgcgc gtattgccga
     4501 cgacgtggct tcacagcagg gagccgatcg ggatctcttg tccgacaagg catggatccg
     4561 gtcggagctg gaacagtttc ccctgcgata cgtctatgga acctctcccc gacgcatcgc
     4621 cgcgcatctt ggcgccattc gccggttgca agtcggtggg gtggtggtgg agacggaatt
     4681 caatgcccca ttgggaacct gcgaatatgc cgtgattgcg cacgacgatc tgatccccgg
     4741 gttgttctcg aagatcgccg gggtgatggc ggccggcgga ttgcagattc tggatgccca
     4801 gatcgtgacg cgaaaggacg gcgtggtcgt cgataccttc caggtggctg atccggacta
     4861 ccagggggct ccgcctgctg accggtgtga atcgattgcc ggcacgatca cggaggtgtt
     4921 gacggggcgt caggccattg agacgctgat gcgtcgcggg accaggctga acatggggcg
     4981 atcgttgcct gcccaccggc agccgtctga ggtgcggatc gacaatgaga cgtccgaccg
     5041 ctttaccatt ctggatgttt ttgcggatga tcggcaggga ttgttgtaca ttatcacgaa
     5101 cgcgattttt cagttggggt tgtcggtgca tgcgtctcgg atctccaccc ggctggatca
     5161 ggtggccgac gtgttttatg tgacgggtat ggatgggaag aaggtggagg aggccggtcg
     5221 gctggaatcg attcgggcgt cgattctcaa tgagatagaa ctgtttctcg gggctcatgc
     5281 ggcgtaaacg acagcgtgtg gtgcggttat tttatcggcg gttgaaagga ggaggggatg
     5341 aacgttcgtg aggtgttaga ttttgcaaag aagaataagg tccaggtcgt ggacatgaag
     5401 ttcgtggacc tgatcggcac ctggcagcat tttacgattc cagtcagtga actgacggaa
     5461 ggcctgttca aggacgggtc cgggctcgac gggtcgtcga ttcgaggctg gagagcgatc
     5521 aacaacagcg acatgctgct cgtgccggac ccggcaaccg cctgcatgga tccgttttgt
     5581 tcggtgccca ccctgagcct catcggcaat gtcgtcgatc cgatcacgcg tgaaatgtac
     5641 gatcgcgatc cgcggttcat cgcccagaag gcggagaaat atctccagag cactaagatc
     5701 ggcgacacct cctattgggg accggaagcc gagtttttca ttttcgatca tgcgcggtac
     5761 gatcagacca accacagcgc ctactaccac atcgattccg acgagggcgt atggaacatg
     5821 gggcaggagg gtgtcaacct gggcggcaag atccgccaca aggaagggta cttcccggtt
     5881 ccgccgaccg atacgcagca ggacatccgc acggaaatga ttctggagat ggagaaggcg
     5941 ggcatcgcca ccgagaagca tcaccacgaa gtggccacgg caggccaggc tgaaatcgat
     6001 atccgcttcg acagcctgct gagaaccgcg gacaagatga tgatgttcaa gtacatcgtc
     6061 aagaacgtgg cgcgtcggca cggcaagacg gtcaccttca tgccgaagcc gattttcggc
     6121 gacaacggat cgggcatgca cacccaccag agcatctgga aagacggaaa gccgctgttt
     6181 gccggaaaag aatatgccgg cgtctctcag atgtgcctgc actacatcgg cggtatcctg
     6241 aagcatgcac cggccctggc agccttcacc aacccgtcga ccaattcata caagcgcctg
     6301 acgccgggtt ttgaagcgcc ggtgttgctg gcctattcga gccggaaccg gtcggccggt
     6361 attcgcattc ccatgtattc tccgagcccg aaggctaagc ggattgaggt gcgctttccg
     6421 gaccccgcag ccaaccccta tctcgcgttt gccgccatgt tgatggccgg attggacggg
     6481 atcgaaaaca agatcaatcc gggagagccg gccgagaagg atctgtacga tctcgaagcc
     6541 aaggaagcgg cgaagattcg cacgatgccg gggagtctgg acgaggccct gaatcacctg
     6601 gagaaggacc atcaattcct gctcaagggc ggggtgttca gcgaggacct cattgaggcc
     6661 tggatcgggt acaagcgtgc caaggaagtg gatacgatgc ggttgcgacc gcatccgtac
     6721 gagttcttcc tgtattatga cgtgtagtga gggtttcctc gtcgagagag cttgacgaat
     6781 ccacgaggcg atggtataca tgcaggggtt gggtgagggc aggcaacgac gcctgaaatc
     6841 acacccgcat gaaatacggt aaacggacaa aggcgtccgt cattcttcca agagtggcgg
     6901 gcgccttttt tattgctgtc gacgctgaag tcggcacaca gcaccgggcg aggtcgtagc
     6961 gaccttgcca agcaggacaa cgacgtcctc ttcccgaatg cgggatgagg acgttttttt
     7021 ttgagttgtt cggacatagt gaccaccgaa aggagagcct catgcataca gcggatcacg
     7081 agcggatcgc aggggtagca gcaaagaagt ggtcattttt cgaacggtca ccgggcggat
     7141 atctcatcct ctcggccttg gccgggatct atctcgggtt tgggattgcg ttgatcttca
     7201 gtgtgggggg accgttggcc gcggccgggt ctccggtggt caaactcgtg atgggggtgt
     7261 cgttcgggat tgcattgacg ctcgtgattt tcgcggggtc tgagttgttc accggcaata
     7321 acctcatcgg caccatcggt ggcctgacga gaagtctcac ctgggcgcaa gtcatgcaac
     7381 tcaatgcctg gtcctggctc ggcaatctgg cgggctcgtt ggggttggcc tggctcattg
     7441 tccaatcggg cgtgtttgcc aaagggccga cgacggagct gatcgagaaa gtggcctcgg
     7501 taaagatgtc gctgcctgcc tgggagctgt tcgtgcgggg cattctctgc aattggctga
     7561 tctgtctggc ggtctggatg acggggcgaa cgaacaacga tacggcgaag attctcctga
     7621 tcttctggtg cctgttcgcc ttcatcggca ccgggttcga gcatagcatc gccaatcaat
     7681 cgttgttggg catggcgttg tttctgccgc atgacgcggc tgtgagttgg gggg
//
LOCUS       DAORQN010000065         7705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_467_length_7705_cov_15.843529, whole genome shotgun sequence.
ACCESSION   DAORQN010000065 DAORQN010000000
VERSION     DAORQN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_467_length_7705_cov_15.843529"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK614_05415"
     CDS             complement(<1..597)
                     /locus_tag="PK614_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB81683.1"
                     /translation="MAKQEKIDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPAEIKHHLDQYVVGQDRAKRILSVAVHNHYKRISAKEKD
                     VDDVELQKGNILVIGPTGTGKTLLAQTLAKFLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRD"
     gene            complement(624..1259)
                     /gene="clpP"
                     /locus_tag="PK614_05420"
     CDS             complement(624..1259)
                     /gene="clpP"
                     /locus_tag="PK614_05420"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRB81684.1"
                     /translation="MLVPIVIEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLII
                     AQLLFLEAEDPEKDINLYINSPGGSVTAGLGIYDTMQYVKPAINTICLGQAASMGAFL
                     LTAGTKGKRYALPNARVMIHQPMGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     LDKISQDTERDYFMSSEEAKRYGLIDEVITRPLKSLKPVGSTDSGKDAGKS"
     gene            complement(1387..2724)
                     /gene="tig"
                     /locus_tag="PK614_05425"
     CDS             complement(1387..2724)
                     /gene="tig"
                     /locus_tag="PK614_05425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRB81685.1"
                     /translation="MNSFMKMEMTELGPMKRALKIEVPADEVNLRFVQAYSELNRQVR
                     IPGFRPGKAPLQLLEKRYAKTIEEDVIRSLVPDYYDRAIRQAGIVPVLVEIPPLERVK
                     VKKDTPFSFTATVEIKPTIELRDYKAPNPISLKQDQRTVSDEQVHKALDVLREQMAQL
                     HPAPAGTTLADGDYAVLDIEGTLDGKPLEGTTKEGHLHKMGSHASVLGIDIESHLPGK
                     KEGEAITIPQAYPETHPDTRVAGKTVSFRCTIKSIKQKQLPALDDEFAKDCGPYQSLQ
                     EIREKLRTEMERALKKDIEDSYKDTILKRLAETHHFELPGTLVERELSAMVRQQLQSR
                     QRKSGDNPDTSPTAPQTEDAKKLQDELRPEAERRVKVGLILEALAAKESITVTNEDLG
                     NEITRLAAEVKLSVEEVTRMIQAGGQDTLDDLRSRILADKALDFVYKHAMIQG"
     gene            complement(2773..2855)
                     /locus_tag="PK614_05430"
     tRNA            complement(2773..2855)
                     /locus_tag="PK614_05430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2819..2821),aa:Leu,seq:tag)
     gene            3183..4268
                     /locus_tag="PK614_05435"
     CDS             3183..4268
                     /locus_tag="PK614_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB81686.1"
                     /translation="MTRSASFAAAIFLLLLLGIGSSAWAYRDYFSETQKALLANIQTV
                     LVEVIALTDTGAGNPEGIREVVTRRMKEMGYAVIADPALPHDVVVRVKCEQRKTWEGT
                     AAAGGDNDLLDAPSRLWKGPACQVTYALGGMKIKWQKEVRTEFEDANQAAQSAQGGAP
                     GPYALTALQVELEKYEFPLFLTAEWGQPDRLLKLMDSPDTSQLRKLKIISLLGEMVAD
                     EALPHLTEALKDKDLAKQAAVALGNMGKEGIPVLIEILKHSKQPDLQAAAAKGLGDLG
                     NTHSDPRVVPPLLEMLDAPGIDIAVQTEIAWALGRVPDRRSVEPLFALDRKLQKIRND
                     PPDPLIKKLKEAVFWSIKQVYTEDQYS"
     gene            4632..5876
                     /locus_tag="PK614_05440"
     CDS             4632..5876
                     /locus_tag="PK614_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB81687.1"
                     /translation="MNRISRRRFLQLSAMTGGALLCGDLVDRVLGASPDQSVASAAEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDLFNKRGGVLGRPVAILEADDASNTETAIK
                     AATKFVRDDRVDVLMGTFNADCALVVSELAKKENKLFMVTGSLLPELTGAACSSHTFV
                     FMPNASMMAQAVVPHLVKAYGTRWYMLTTSSLDGKAMAQAMVAAGQAHGVEFVGEALV
                     PFGATDFTAAFTAAKDKHPTLVVLNLYGWDLVHALKTYTKLEYAKEKIGVGGMIAGEQ
                     IGRPLGYANNAGIWGLIWDPKVNTEGSRQFIRAVVDKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGTTDAAALIKALEGREFDGLKEGRSYFQASNHQHMQDVLVGEAYGKELGLGHY
                     KILATVPGDSLAAPVHAGVCQL"
     gene            complement(5941..7446)
                     /locus_tag="PK614_05445"
     CDS             complement(5941..7446)
                     /locus_tag="PK614_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB81688.1"
                     /translation="MRRAVWALMVLSSLAAGCETAPHVQPIASSPATVEPKLLALQQE
                     REQLLTTLGEFHDRIRDLESKLGDRQSQPAAASYDQLLSAKEAELVELRRLAPERDRL
                     TGQLTTAANELIQARQRIGTLEQQLATRDKDLAALQTRATAVADLEVARRRISELEAH
                     IARQDRDLHTVRTGSAERDSLAAQLQTATATIESLKARISALDQQLKDREQAYETVRS
                     RLMERDKLVPQYNAMIAEIYQARHRIAALEQRLNEKGRDLSPRQKGTQLTTSQRDGGP
                     LNQRSGAANQSLGDTAKSIPDGKSAPQGKDSTRSGVAFPGNPAQAEARSGSFAAVKEE
                     LLKVLPGDNRQKTISMRQDGNRLTVALDSNLLFTSGDAALSPEGATILKRIGAVLGQL
                     SDKFVQVAGHTDNQALSKTLQKTFPDNKALSWARAENARRALVNGGIPADRTKAVGLA
                     DTRPLTSNATEQGRQKNRRLELIIVQGPTVAATVEESRRGQVRLATLSATP"
BASE COUNT         1473 a         2008 c         2332 g         1892 t
ORIGIN      
        1 atccctcgtg atggaagggc tatcgctttt tctactaatc ttgtcgattt cgtcgatata
       61 tacaatcccg cgctcagcgc gttcgacatc gtagtcggcg gcctgaagca gttttaggat
      121 aatgttctca acgtcttcac cgacatagcc tgcttctgtg agcgtggtgg catccgcaag
      181 ggtgaacggc acgtcaagga acttggccag ggtttgagcc aggagtgttt ttccggtgcc
      241 ggttgggccg atgaccagaa tattgccttt ttggagttcg acatcgtcga cgtctttctc
      301 cttggcggag atgcgcttgt agtggttgtg tacggcaacg gagaggattc gcttggcacg
      361 atcctgcccc accacgtatt gatcaaggtg gtgtttgatt tcggcaggtt tcttcagctt
      421 cgaagatatt tcttccttag cctcttccca atcctccgca atgatgtcgt tgcagaggtt
      481 gacacattca tcgcagatgt agacggtggg accggcgatg agcttacgca cttcgtcacg
      541 gcttttcccg caaaacgagc atcgcaggtg ccgatcaatc ttttcttgct tagccatgct
      601 gcctcctgta caccaaaggt caactagctc ttgccggcgt ccttccctga gtccgtggac
      661 cctacgggct tgaggctctt cagggggcgt gtgataactt catcgatcaa tccataccgc
      721 tttgcttctt cgctcgacat gaaatagtct cgctcggtgt cctgcgaaat tttgtcgagt
      781 ggctgcccgg tgtgtttcgc catgatttcg ttcaggcgtt cgcgaatctt caggatctcg
      841 cgcgcgtgaa tatcgatttc cgtggcctgg ccctgaaatc ctcccatcgg ctgatggatc
      901 atgactctgg cattcggcaa ggcgtagcgc ttgccctttg tgccggctgt caggaggaag
      961 gcgcccatgc tggcagcttg gcctaagcag atcgtattga tggcaggttt gacgtattgc
     1021 atcgtatcgt agatgccaag tccagccgtg acacttcccc cgggggaatt gatgtacaga
     1081 ttgatatcct tctcagggtc ttcggcttcc aggaacagca gttgagcgat gatcaggttt
     1141 gcaaagacgt catcgatcgg cgcgccgaga aagatgatgc ggtctttaag caggcgtgag
     1201 taaatgtcat aggcacgttc gccgcggttc gtctgttcga tgacaatcgg aactagcatt
     1261 cagctgaatc ctttccgcta gaggcctgac aggtggaaac tcttcagttt accatactca
     1321 attaacctgg attttggtga tcaccgccca gctatccagt gctgcgactc atggcagccg
     1381 actaggctac ccctggatca ttgcgtgctt gtagacaaaa tccaaagctt tatcggccaa
     1441 aatacgagat cgaaggtcgt ctagggtgtc ttgcccaccg gcttgaatca tgcgggtgac
     1501 ttcttcgacc gagagtttga cctccgcagc gagcctcgtg atttcgttgc caagatcctc
     1561 attcgtgacg gtgatgcttt ccttcgccgc cagtgcctca aggataagtc caaccttgac
     1621 gcgccgttcg gcctctgggc gcagttcatc ctggagtttc ttggcgtcct ctgtttgagg
     1681 cgcggtaggt gaagtgtcgg gattgtcgcc ggatttccgc tggcgggatt gcaattgttg
     1741 ccggaccatg gctgacagct ctcgctcaac cagtgttccg ggtagttcaa aatgatgggt
     1801 ttcagccagt cgctttaaga tggtgtcctt gtagctatcc tcgatatctt tcttcagggc
     1861 ccgttccatt tcggtgcgaa gcttctctcg gatttcctgc aatgattggt agggcccgca
     1921 atcctttgcg aactcgtcgt ctagcgcggg aagctgtttc tgctttatgg acttgatggt
     1981 acagcgaaag ctcacggtct tgccggcgac gcgagtatca gggtgtgtct cgggataggc
     2041 ttgtggaata gttatcgcct caccttcctt tttacctggg agatgagatt caatgtcgat
     2101 gccaaggacg gaggcatgtg agcccatttt gtgaaggtgc ccttctttgg tcgttccttc
     2161 gagtggcttg ccatccaagg ttccttcaat atctaagacc gcatagtcgc catcggccaa
     2221 tgttgttccg gcaggagcag ggtgtaattg ggccatttgc tcgcggagaa cgtcgagggc
     2281 tttgtggacc tgttcatccg agacggttct ctggtcctgc ttgagggaga taggattcgg
     2341 agccttgtaa tctcgaagtt cgatggtcgg cttgatttca acggttgcgg taaagctaaa
     2401 gggcgtgtct tttttgactt tgacccgttc cagcggagga atttctacca gaaccggaac
     2461 gatgcccgcc tggcggatgg ctcgatcata ataatccggg accaggctgc gaatcacgtc
     2521 ctcttcgatc gtctttgcat atcgcttctc aagtaactgg agcggagcct tgccgggtct
     2581 gaatccggga atgcgaacct gccggttcaa ttcagagtag gcctgaacaa agcgcaggtt
     2641 cacttcatct gccgggactt caattttgag ggcccgcttc atggggccga gttcggtcat
     2701 ttccattttc ataaagctgt tcacgtcctc cgggtccgaa aagttatttg cgccatacgg
     2761 atctgtgaga ggtggtgcga gaggggggac ttgaaccccc acggttaccc acgagatcct
     2821 aagtctcgcg cgtctgccag ttccgccact ctcgcgcgac gctgagtact aggttgctca
     2881 ccgatgctac ataaggaatg tgcctgcgcg tggaaagaca cgatgaataa tgacattttg
     2941 taccgtcgaa cgagtctgcc tgtcaaccca tacgccggtg gggcctgcgt gttggcctgt
     3001 cggtcggagt ggaaaaagga tgctgatgat aacgtggctg ggcgggggac tgtgggctgt
     3061 cggttcaggt tgatacttgg cgttccttcg tagagtcggg aggccttgga gcgtataatg
     3121 aagtgtcgat tctttgttgc atggaccttc atcaagaccc tattggaaag gaggaagtcc
     3181 atatgacgcg gagcgcttcc tttgccgcag ctatcttcct tctgcttctc cttggcatcg
     3241 gctcttcagc atgggcctat cgggactact ttagtgaaac gcaaaaggcg ctactagcaa
     3301 atatccagac ggtcctcgtt gaggtgatcg cgcttactga tacgggcgct ggtaaccccg
     3361 agggaatcag agaagttgtc acgcgacgca tgaaggaaat gggctatgca gtcattgcag
     3421 atcccgccct tccgcacgat gtcgtcgtcc gggtaaagtg cgagcaacgt aagacgtggg
     3481 aaggtaccgc ggcggccggt ggagacaatg atttactgga tgccccttct cgcctgtgga
     3541 agggaccggc gtgtcaggtg acatatgctt tgggagggat gaagatcaaa tggcagaagg
     3601 aggttcggac ggaatttgaa gatgccaatc aggccgctca atcggctcag ggtggcgcgc
     3661 ccggtcccta tgccctgacc gcgctccagg tcgagttaga gaagtatgaa tttccgctct
     3721 tcttgacggc cgaatggggg cagccggatc gactgctgaa gttgatggat tctccggaca
     3781 ccagccagct ccggaaattg aagattattt ccctcctggg agagatggtg gccgatgaag
     3841 cgttgcctca tctgacagag gcgctgaagg ataaggactt ggcgaagcaa gcagcggtgg
     3901 cgttgggcaa tatggggaag gagggaatcc cagtgctcat cgagatcctg aagcactcca
     3961 aacagcctga cttgcaggca gcggcggcta aggggttggg cgatcttggg aatacccata
     4021 gtgacccgag agtggttccg ccgcttcttg agatgttgga cgcacccggc attgacatcg
     4081 ctgttcaaac ggagattgcc tgggcattgg gacgggttcc cgatcggcga tcggttgagc
     4141 cactgtttgc actcgatagg aaactgcaga agatccgcaa cgatccccca gaccctctga
     4201 tcaagaagct gaaggaggca gtgttctggt cgattaagca ggtctacacc gaagaccagt
     4261 acagctaacc tgcgattcac accctcccag ttcttccttg ccgttccagg ggcggttacc
     4321 gggatggagc ctaaggtgtg tgttctcagc cccgcttcct cctcttgccg caactagcta
     4381 gggttatttg agcccatcgt actccaggaa cattctcgcc cagcccttcc agctgttttg
     4441 acgaccagca tttgcgaggg ccatacctcg cccattgtga gaggaggtcg ttgtccctga
     4501 ggtccaactt cgtgctgtga aaacgtagat gactctgtta agagagacaa tccctctcgg
     4561 tgtgatcatg gccttaccat cggggtcgtg gtgcctggga gggcgaatga aaaccagggg
     4621 ggatcaagta tatgaatagg atctcacgca gacgtttctt gcagctatca gccatgaccg
     4681 gcggcgcgtt gctctgtggc gacttggtcg accgtgtgct cggtgcgtcg cccgatcaat
     4741 cggtggcctc tgccgcagag ccgatcaaga tcgggatcct cgatccgctc tcgagcccgt
     4801 acaagacctc ttccattcat gatgtgcatg gggccaatgt ggccgtcgat ctgttcaata
     4861 aacgtggcgg tgtgttgggg cgaccggtgg cgattctcga agccgatgat gcgtcgaata
     4921 ctgagactgc catcaaagcc gccaccaagt ttgtgcgaga tgatcgagtt gatgtcctga
     4981 tgggcacgtt taatgcggat tgtgcgctcg tggtttcgga gttggcgaag aaggagaaca
     5041 agctgttcat ggtcacgggc tcccttctcc ccgaactgac cggagccgcg tgcagctcgc
     5101 ataccttcgt gtttatgccg aatgcctcga tgatggcgca ggctgtcgtg ccccacttgg
     5161 tcaaggccta cggcacccgc tggtatatgc tgacgaccag ctcattggac ggcaaagcca
     5221 tggcgcaggc catggtggcg gccggccagg ctcatggcgt tgagtttgtg ggggaggcac
     5281 tggtgccttt cggggcgacg gatttcactg cggccttcac ggcggccaag gacaaacatc
     5341 cgacgctggt ggtgctcaat ctctatgggt gggatttggt ccacgcactg aagacataca
     5401 cgaagcttga gtacgcaaaa gagaagatcg gcgtgggtgg gatgattgcg ggtgaacaaa
     5461 ttggacggcc cctcggttat gccaacaatg ccggcatttg ggggctcatc tgggatccaa
     5521 aggtcaacac ggaaggatcc aggcagttta ttcgagcagt cgtcgacaaa tacaatcaca
     5581 cgccaacttc gcgctgctat cttgggtatg ccgccatgac gcagatcctt gaggcgattc
     5641 aacgagcagg aacgacggac gctgccgcac tcattaaggc gttggagggg cgcgagtttg
     5701 acgggctgaa agagggacgg tcctacttcc aggcctcaaa ccatcagcat atgcaggatg
     5761 tgctggtcgg ggaagcctac ggaaaggagc tggggcttgg gcattacaag attctggcga
     5821 cggttccagg cgacagtctt gcggcgcctg ttcacgctgg tgtctgtcag ttgtagcgaa
     5881 caggccgtga tgtgatggtg gcgagtcgtc agtacgcggc gattccgttt gtgtgacagg
     5941 ctaaggtgtt gcgctgaggg tcgcgagtcg aacctgcccg cgcctcgatt cctcgaccgt
     6001 cgcggcaaca gtcggcccct ggacgatgat caattcgagg cggcgatttt tctgcctgcc
     6061 ctgctcggtg gcattggatg tgagcgggcg tgtatcggcc agcccgacgg ccttcgtcct
     6121 gtcggcgggt atccctccat tgaccagtgc cctgcgggca ttttcagcgc gagcccatga
     6181 gagcgccttg ttgtcgggga aggttttttg aagcgttttg ctgagtgctt gattgtcggt
     6241 gtgtcctgcg acctgcacga acttgtctga aagctgtccc aggaccgcac cgatgcgttt
     6301 gaggatagtt gcgccttccg ggctcagcgc agcgtcaccc gatgtgaaca gcaagttgct
     6361 gtccagcgcg accgttaatc gatttccatc ctgcctcatg ctgatcgttt tctgtctatt
     6421 gtcgcccggc aggactttga gaagctcttc tttcaccgcc gcaaagcttc cgctccgagc
     6481 ctcagcctga gcggggtttc ctggaaatgc aacaccgctg cgtgtggagt cctttccctg
     6541 gggggctgat ttcccatcgg gtatcgattt ggccgtgtcg ccaagagatt ggttggcagc
     6601 gccggagcgt tggttcagcg gtcctccgtc gcgctgactg gtggtgagtt gagtcccctt
     6661 ctgccgcggc gacagatctc gccccttctc gttcaggcgc tgttcgagcg ctgcgatgcg
     6721 gtgtctggcc tggtagattt ctgcaatcat cgcattgtat tggggcacta gcttgtcgcg
     6781 ttccatgagg cgtgatcgaa ccgtttcata ggcctgctca cggtctttga gttgctgatc
     6841 gagtgcgctg atacgggctt ttaaggattc gatggtggcg gtggcggttt gtagttgagc
     6901 cgccaggctg tcccgttcgg cggaccccgt acgaactgtg tggagatcgc ggtcttgacg
     6961 ggcgatgtgc gcttccagct ccgagatcct gcgtcgggcg acttccaggt cggcgacggc
     7021 cgttgcccgg gtctggaggg cagccagatc tttgtctcgc gtggccagct gttgttcaag
     7081 cgtcccgatt cgttggcggg cctggatgag ctcgttggcg gcggtcgtca attggccggt
     7141 gagccgatcg cgttccggtg ctaaccgcct cagttcgaca agttccgctt ccttcgcgct
     7201 gagcaattgg tcgtacgatg ccgcggcggg ttgactctgg cggtctccca gtttgctttc
     7261 gaggtcgcga atgcgatcgt ggaattcacc cagcgtggtc aacagctgtt ctcgctcctg
     7321 ttgaagggcc agcagcttcg gttctacggt cgctgggctc gatgcgatgg gctggacgtg
     7381 cggagcggtt tcacagcctg cggcgaggga actgagaacc atgagcgccc atactgctcg
     7441 tctcatacga tctctccccg gtcattgata ggaagacggc gccggttgca tgggggcgac
     7501 agtcgcccga gaggagccac cgcagtcaag aaaagaaggc gactgtgacc gagctgacgg
     7561 atgatgatca gctggtgtga gccatgcgtc ccgacagaat ctgttgagtg acatgcaggc
     7621 cccaggaaca cgacagcaat ctatcgcgtt cctggggctg atgcaaggct ggtggagctg
     7681 cgggtagccg gttagtgtcc gacga
//
LOCUS       DAORQN010000066         7697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_469_length_7697_cov_7.280817, whole genome shotgun sequence.
ACCESSION   DAORQN010000066 DAORQN010000000
VERSION     DAORQN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_7697_cov_7.280817"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /gene="zapB"
                     /locus_tag="PK614_05450"
     CDS             complement(<1..226)
                     /gene="zapB"
                     /locus_tag="PK614_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HRB81689.1"
                     /translation="MTLDHLDALEIRIRDLVKLVQDLKRKNASLEDDLRLARERVAVR
                     DDENRRWEQERIDIRSRIEKVLGEIDLLECL"
     gene            complement(321..2558)
                     /locus_tag="PK614_05455"
     CDS             complement(321..2558)
                     /locus_tag="PK614_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB81690.1"
                     /translation="MTALPWRINLSALAVWSVLSSLFLTATAESPRPLPVATAPCVSA
                     EDCFRSAVALNERSGSPVQRDQTMMLKIDQLRSVMELYPSTIWAKRAGVVLGVLRIER
                     EPVEAAKLLRVVQPDMPVLDDYLRLWIGESLLKQNEPIQAAELLETIPKIVPDSSLIA
                     KAAYRTGEAWYSANVCFRAVDWLERAVALADKDPAAPLALWHLAECHIRENRLPEART
                     ALKQLWLRYPHSPEAREAEARLDTALGGESWTPTAEDHSIRAQAFLGLAMQAEAVEEL
                     RRFLAMAPGHPRRFDARLKLGVAYVRLKQYDQARETFRGLVADRVQESSEATVWLARV
                     YLRQNQGDKLIALARSIAQGSLGGDQRAMVHLFAGVWLEDQGQFDEAIGMFRQVAKLG
                     DSASQRAEGLWRAGWAQYRTARYRDAAETFRSVVELHVNGFEPQAMYWAARADEREKN
                     TTVADQYTRLCQRHAYSYYCQLAARRVSLPPVTPVATDAERPAGDEASRLPENRRPEI
                     ERHVVYQRGIELKILGFAQDAARELGSLTEQYSRDPEVLLAFSTLLSEVGAYHPALRV
                     AKVHFKEKLERSGLPTAPALWTVAYPTGLLPTITAQGVTAVDPYLAAAIIREESQYDE
                     KAVSMVGAVGLMQLMPVTANAVAQRYGFPAVGREELFDQETNIRLGVRYLGQLLEQYS
                     GNLAHAVAAYNAGPIAVNNWIAVHRGREQDEFVELIPYQETRLYVKRVLRSYGEYRRL
                     HNGTS"
     gene            2670..3758
                     /gene="rlmN"
                     /locus_tag="PK614_05460"
     CDS             2670..3758
                     /gene="rlmN"
                     /locus_tag="PK614_05460"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRB81691.1"
                     /translation="MLQRTDNRTNLLAVTENGMAAFVASLGWPAYRASQILRWLYQER
                     ARTFTEMSNLSQKDREYLTGNCSIERTSAVQIFSSQDGTKKLVLTLDDGNQVECVLIP
                     DEDRLTLCLSTQVGCTLDCGFCLTGTLGLQRNLRAHEILDQVLLAQDHLSEGERLTNL
                     VFMGMGEPLANLDAVADAVTRLTNNTWGLGFSGRRITISTAGLASRIKDVAPLKVNLA
                     ISLNATTDTLRQQLMPAANRLHSLDALLAACRAYPLADRDRLTFEYVLLADVNDRAED
                     AARLVKLLRGLRCKVNLIAFNPFPGNPYRRPSDAAIEAFQNTLRRGHVDVYLRRSRGR
                     DVLGACGQLGRIDASDAQVALTQIQTRC"
     gene            3809..5164
                     /locus_tag="PK614_05465"
     CDS             3809..5164
                     /locus_tag="PK614_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB81692.1"
                     /translation="MRFSILACSLLSFILLASQTSPAHAVEPKEYTLSNDMKVILVEV
                     PKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGTFSRLVRKNGGMDN
                     AFTSQDFTAYFENLAADRVTLALELEADRMQGLILDANEFKTEREVVKEERRLRNEDD
                     PQGALVEALFAQAFMSHPYHWPVIGWFSDLDAMNLDDLQRHYDTYYSPNNATLIVVGD
                     IKADTLLPTIAKLFEPIPKGPSPKPLAVTEAPQHGERRFLLKREAQVPFVMMGYRVPN
                     YSSDDSYALNVLESILSHGKSARLYQSLVYEQKTALAVGADYGLMQADPGLFYFYAVV
                     KPGEKVEAVEDAVLKEIQRIQAEPPSELELQRAKNQIEAAHIFEQDSNFRQAMLLGEA
                     ETIGAGWRKVSQFVERTRAVTAQDVQRAASHYLAADLRTTGTLIPQPPQTQAASPAQP
                     H"
     gene            5177..6559
                     /locus_tag="PK614_05470"
     CDS             5177..6559
                     /locus_tag="PK614_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB81693.1"
                     /translation="MTQQPATLLPRQHRSRRPVGRMLAGLLCMALSLPFALAQAADIV
                     PTRSVTANGMTVLFLEQHFLPTVEIHALVKVGSAQDPPDKAGLANLTASLLDEGTLTR
                     TSRQIAEQIDFVGGSLEAHAAEDFTTASTRVLKKDADLGFALLADMLQHPAFHKQEFE
                     RVRTQILGEIVSDDDDPGNVAMKAFHQLIFHGHPYSWPAHGTEETLNKITVTDIQQFH
                     AREYLPNQTILVIVGDLTQDQAAALVQTHFGSWKKGPPSPYQIKKPASIERKMVQLIE
                     KDLTQSTIVLGHTGISRTNPDYYAVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSQ
                     FDTRLMPGAFFISLQTRTDVTNQAIAGVLTEIKGMRDAPVTDQELNEAKSFIVGSFPL
                     RVDSSAKLANVLAQVELYNLGLDYFTQYPKAIEKVTKDDVLRVAKQYLDPQHYALVVV
                     GSIAKAKVKQ"
     gene            6603..7424
                     /gene="larE"
                     /locus_tag="PK614_05475"
     CDS             6603..7424
                     /gene="larE"
                     /locus_tag="PK614_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRB81694.1"
                     /translation="MVSTTPLIDKVSQARQILREMGSVIVAFSGGIDSSLVLKLAHDE
                     LGAQAVGITAVSPTLPASELDATQKIAAEIGARHRIVETDQLQIPEFVLNDATRCYHC
                     KTDLYSLLETLRSEYADGYIVDGTNVDDLGDDRPGLKAARERGVRSPLLEAGFSKADI
                     RDVARELHLSNWDKPAAACLSSRIPRGITITRSTLSRVERAEDALAQEGFRQYRVRDH
                     GEIARIELAVEELPGLLQPGRRERLAETLKKLGYRFVTVDLEGYRQGGVSLTPPA"
     gene            complement(7431..>7697)
                     /locus_tag="PK614_05480"
     CDS             complement(7431..>7697)
                     /locus_tag="PK614_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81695.1"
                     /translation="AQAKERAQRLMEENEQRMTADAERIKGDATREIETERRKAIQDI
                     RSQTTDLAMMVAEKVVGRALTEGDHRRLADEALDALAKSYQSRN"
BASE COUNT         1609 a         2517 c         2102 g         1469 t
ORIGIN      
        1 ccaggcattc caacagatcg atctcgccca ggactttctc gatccgtgaa cggatatcga
       61 tccgctcctg ttcccagcgt cggttttcgt catcacgaac cgcaacccgc tcccgcgcca
      121 gacgcaagtc atcctctaat gaggcatttt tccgcttgag atcctgcacc agcttcacca
      181 aatcgcgaat ccgaatttca agggcgtcga gatgatctaa cgtcatggta tttcctcata
      241 tcaaagtggt ggccgaacac gttgggtgac gggggcgaaa tatagaaagt tcgctccggc
      301 ttgtcaagaa aacggccccg ctacgacgtg ccgttatgga gacggcgata ttctccatag
      361 ctgcgcaaga cccgtttcac gtacaaccgc gtctcctgat acggaatgag ttccacgaac
      421 tcgtcctgct cccgtcctcg atgtaccgcg atccaattgt tcacggcaat cgggccggcg
      481 ttgtaggccg cgacggcatg ggccaggttc ccgctgtatt gctcgagcaa ctgccccaga
      541 taccgcaccc ccagtcgaat gttggtttcc tgatcgaaca gttcttcccg tccgaccgcg
      601 ggaaacccgt accgctgtgc cacggcattc gccgtgaccg gcatcaactg catcaacccc
      661 acggctccca ccatcgagac agccttctca tcgtactggc tctcctcgcg aatgatcgcg
      721 gcggccaggt aggggtcaac cgcggtgaca ccctgcgcgg tgatcgtcgg gagcaggccg
      781 gtcgggtagg ccacggtcca caaggcgggc gcggtcggca aaccgctgcg ttccagcttc
      841 tccttaaagt gaacttttgc cacgcgtaag gctggatggt acgcccccac ttcactgagc
      901 aacgtcgaga aggccagcag cacctccggg tcacgactat actgctctgt gagagagcca
      961 agttcgcgag ccgcatcctg ggcgaacccc aatatcttga gctcgattcc acgttgatag
     1021 accacgtgcc gttcgatctc aggacgccga ttctccggca atcgtgaagc ctcatcgcct
     1081 gccggacgct ccgcgtctgt cgcaaccggc gtgaccggcg gcaatgaaac tcgccgtgca
     1141 gccaactggc agtagtaact gtaggcgtgc cgctgacaga ggcgtgtgta ttgatcggca
     1201 acggtggtat ttttttcgcg ctcgtcggcc ctcgccgccc aatacattgc ctgaggctca
     1261 aagccgttga catgcaactc cacaacggat cgaaacgtct cggctgcgtc ccgataccgg
     1321 gctgtccgat attgcgccca cccggcacgc cagagacctt ccgcacgctg actcgccgaa
     1381 tcccccagct tcgcgacctg ccggaacatg ccgatcgcct catcgaattg cccctgatcc
     1441 tccaaccaca ctccggcgaa gaggtgcacc atcgcccgct ggtcgccccc aagggacccc
     1501 tgcgcgatcg atcgcgccaa ggcgataagc ttgtcgccct gattctgccg cagatagacc
     1561 cgcgccaacc ataccgtggc ctcggaggat tcctgcaccc gatccgccac taacccgcga
     1621 aacgtctcac gggcctggtc atattgcttg agccggacgt aggccacgcc caacttcaag
     1681 cgggcatcaa accggcgggg gtgcccggga gccatcgcca gaaagcggcg cagttcttcg
     1741 accgcctccg cttgcatggc cagccccaga aaggcctgtg cgcgaatcga atgatcttcg
     1801 gccgttgggg tccaggattc tccgcccagt gccgtatcca accgggcttc cgcctcccgc
     1861 gcttcaggcg agtggggata gcgaagccag agttgcttca acgcggttcg cgcctccggc
     1921 agtcgattct cccgaatgtg gcactcggcc aggtgccaca aggccaacgg agccgccggg
     1981 tccttatccg caagcgccac agcccgctcc agccagtcca ccgcccggaa gcacacattg
     2041 gcgctatacc aagcttcgcc tgttcggtag gcagccttgg caatcaggct cgaatccggc
     2101 acgatcttgg gaatcgtctc cagcaattcg gccgcctgga tcggttcgtt ctgcttcagc
     2161 agcgattccc cgatccagag gcggagatag tcgtcgagca ccggcatgtc cggctggaca
     2221 acacgcagaa gtttcgccgc ctcgaccggc tcccgctcga tccgcaagac gcccagcacc
     2281 accccggccc gcttggccca gatcgtggaa ggatagagct ccatcaccga ccgcagctgg
     2341 tcgatcttca gcatcatcgt ttgatcgcgt tgaacgggcg atcccgaacg ttcattgagc
     2401 gccacggcgg aacggaaaca atcttccgcg gagacgcagg gtgcagtggc aacaggcagg
     2461 gggcgcggtg actcggctgt ggccgtcaag aacaggctcg acaacacaga ccacacggca
     2521 agagccgaca agttgatccg ccaaggaagt gcggtcacat tctcatcctg agaagagggt
     2581 gaaccggccc tattatagcc gaacgcggca taaacgcgac cacgttcgcc ttgcttgtca
     2641 caagtttttt ccctgtgcta ggctccggta tgctccaacg gaccgataat cgcaccaatc
     2701 ttctggccgt gaccgaaaac gggatggccg cattcgtcgc atcactcgga tggccggcct
     2761 atcgcgcgtc gcaaatcctg cgctggctct atcaggaacg tgcccgcacg ttcaccgaga
     2821 tgagcaatct ctcgcagaag gaccgggagt acctgacggg caattgcagt atcgaacgga
     2881 cttcggcggt ccaaatcttt tcgtcgcagg atggcaccaa gaaattggtg ctcacgctgg
     2941 acgacggcaa tcaagtcgaa tgtgtgctca ttcccgacga ggaccggctc accctctgcc
     3001 tgtccacgca ggtcggctgc acgctcgatt gtggattctg cctgaccggt acgctgggac
     3061 tccaacgcaa cctgcgcgcg catgagatcc tcgaccaggt cctccttgct caggaccatt
     3121 tgagcgaagg cgaacggttg acgaacctgg tcttcatggg catgggcgaa cccctcgcca
     3181 acctggatgc cgtggccgat gccgtcaccc gcctcaccaa caacacctgg ggcttggggt
     3241 tttcaggtcg ccgcatcacg atctccaccg caggactggc ctcgcgaatc aaggacgtgg
     3301 cccccctcaa ggtgaacctg gccatctcgt tgaatgcgac caccgatacg ctccgccagc
     3361 aactcatgcc cgcggccaac cgactccatt cgctcgacgc cctgctcgcg gcctgtcggg
     3421 cctatccgct ggcggaccgc gatcgcctga cctttgaata tgtcctcctc gcagacgtga
     3481 atgatcgtgc cgaggatgcc gcccgcttag tgaagctgct tcgtggcctc cgctgcaagg
     3541 tcaacctgat cgccttcaac ccgtttccgg gcaatcccta tcggcgcccc tcagacgcgg
     3601 ccattgaagc gtttcaaaac actcttcgcc ggggccacgt agacgtgtac ctccgccgaa
     3661 gtcgtggccg cgatgtgttg ggtgcctgcg gccagctcgg tcgtatcgat gcgtcagatg
     3721 cccaggttgc cttgacacag attcagaccc gttgttagca tgttcttcgt acatgcctaa
     3781 ccagagcaca acttccaact cagcacctgt gcggttctcc atcctcgcct gttcattgct
     3841 aagcttcatc ctgttggcga gccagacctc ccccgctcat gccgtcgagc ccaaggaata
     3901 caccttgtcc aacgatatga aagtgatcct cgtggaagtc ccgaaggctc ccgtggcgac
     3961 cgtgcaagtc tggtacaagg tgggctcacg aaacgaggtt atgggccggg ccgggttgtc
     4021 gcacatgctt gagcatatga tgttcaaagg cacggccaag taccccaaag gcaccttctc
     4081 ccgcctcgtt cgcaagaacg gcggaatgga caatgccttc accagccagg acttcaccgc
     4141 ctactttgaa aacctggccg ctgatcgcgt gacgctcgca ctcgaactcg aggccgaccg
     4201 catgcaaggg ctgattctcg atgcgaacga attcaagacc gaacgggaag tggtgaaaga
     4261 agaacgccgg ttgcgcaacg aagatgatcc gcaaggcgcg ctggtggaag ccctcttcgc
     4321 acaggccttc atgagtcacc cgtaccactg gccggtcatc ggatggttct cggacctcga
     4381 cgcgatgaac ctcgacgatc tccagcgtca ctacgacacc tactattcgc cgaacaacgc
     4441 caccctgatc gtggtcggcg acatcaaagc ggacacgctc cttccgacta ttgccaagct
     4501 cttcgaaccg atcccgaaag gtccgtcccc caaacccctg gccgtgacgg aagccccgca
     4561 acacggcgaa cgccgatttc tcctcaagcg agaagcgcag gtgccgttcg tcatgatggg
     4621 ctaccgtgtc cccaactatt cgagcgacga ctcctatgcc ttgaacgtac ttgaatcgat
     4681 tttgtctcac ggcaagagcg cccggctcta tcaaagcctc gtgtacgagc aaaagaccgc
     4741 cctggcagtc ggcgccgact atggcctgat gcaagccgac ccgggattgt tctactttta
     4801 tgccgtggtg aaaccaggcg agaaggttga agcggttgaa gacgcggtgc tcaaagagat
     4861 tcagcggatc caagccgagc cgccgagcga actcgaactg cagcgcgcga aaaatcagat
     4921 cgaagcggcc cacatttttg aacaggattc gaatttccgc caggccatgc tgctgggaga
     4981 agccgaaacg atcggggccg gatggcgcaa agtcagccag tttgtggagc gtacccgcgc
     5041 cgtcaccgcg caggacgtgc agcgtgcggc gtcgcactac ctggccgccg atctgcgcac
     5101 cacgggaacc ctgattcctc aacctccgca aacccaggcc gcgtcaccgg cacaacctca
     5161 ctagcgagcg atcccgatga cgcaacaacc agcgaccctt ctgccacgcc aacaccgaag
     5221 ccgccggcct gtgggccgga tgttagcggg cctgctctgt atggcgctca gccttccgtt
     5281 cgccttggcg caagctgcgg acatcgtccc cacccgctcc gtgaccgcga acggcatgac
     5341 cgtgctgttt ctggaacagc attttcttcc gaccgtggaa atccacgcgc tggtcaaggt
     5401 gggatcggcg caggatccgc ccgacaaagc aggtctggcc aatctgaccg ccagcctcct
     5461 cgatgaaggc accctgacca ggacatctcg acaaatcgcc gaacagatcg attttgtggg
     5521 aggctcgctg gaagcccatg cggcagagga tttcaccacc gcctccaccc gcgtgctcaa
     5581 gaaggatgca gacctcggct ttgcgcttct ggccgacatg ctccaacatc ctgcgttcca
     5641 caagcaggaa ttcgaacggg tccgcacaca gatcctcggc gaaatcgtca gcgatgacga
     5701 tgatcccggg aatgtggcca tgaaggcctt ccaccaattg atttttcacg gccatcccta
     5761 tagctggccg gcccatggca cggaagagac gctcaacaaa attacggtca cggacatcca
     5821 gcaatttcac gccagagaat acctgccgaa tcaaacgatc ctggtcatcg taggggacct
     5881 gacacaggat caggccgccg ctctggtcca gacccatttc ggctcctgga aaaagggacc
     5941 cccctcgccc taccagatca agaagccggc ttcgatcgaa cgtaaaatgg tccagctcat
     6001 cgagaaggat ctgacgcagt cgacgatcgt ccttgggcac accggcatta gccggaccaa
     6061 tcccgactac tatgccgtca cagtcatgaa ctatatcctg ggcgccggtg ggttctcctc
     6121 ccgcctcatg gattccattc gcgacaaaca gggactggcc tatggaatca tgagccagtt
     6181 cgacacccgc ttgatgccgg gagccttttt tatcagcctg cagacccgaa ccgacgtcac
     6241 aaaccaggcc atcgcgggcg tcctcacgga gatcaaaggg atgcgcgatg ccccggtcac
     6301 ggatcaggaa ctcaatgagg ccaagtcctt catcgtcggc agcttcccgc tgcgggtcga
     6361 ttccagcgcc aaactggcaa acgtcctcgc ccaggtcgaa ctctacaacc tcgggttgga
     6421 ctacttcacg cagtatccta aagccatcga aaaggtcacg aaggacgatg tcctacgcgt
     6481 cgccaagcag tatctcgacc cccaacatta cgccttggtc gtcgtcggat cgatcgcgaa
     6541 ggccaaagtc aaacaatagc cggccactct cccgcagccg ggccacgcag ctctagaatc
     6601 ccatggtttc cacgacgccg ctgatcgata aagtctctca agcacgacag atccttcgag
     6661 agatgggctc ggtcatcgtg gccttctccg gcgggatcga cagttccctc gtcctcaaat
     6721 tggcgcatga tgaactgggt gcccaggccg tcggcattac ggctgtctct cccacccttc
     6781 cggccagtga gttggacgcg acgcagaaaa tcgccgcaga gatcggcgca cgccatcgca
     6841 tcgttgaaac ggatcaactc caaattcctg aattcgtcct caatgatgcc acacgctgtt
     6901 accactgcaa gactgacttg tactcactcc tcgaaacgct ccgaagcgaa tatgcggatg
     6961 gatacattgt cgatggcaca aacgtggatg acctgggaga cgaccgtccc ggcctcaagg
     7021 ccgctcgcga gcgaggcgtc cgtagcccgt tgctcgaagc cggattctcg aaggccgaca
     7081 tccgcgacgt agcgagagag ctacacctct ccaattggga taagccggca gccgcctgcc
     7141 tctcttcccg cataccacgc ggcatcacga tcacgagaag cacactttca cgcgtggagc
     7201 gtgcggaaga tgctctcgca caagaaggct ttcgccaata tcgtgtgcga gaccatgggg
     7261 agatcgccag aatagaactg gccgtagaag aactgccggg gttgttgcag ccgggacgcc
     7321 gggaacggct ggccgaaacc ctgaagaaac tcggctatcg cttcgtgacg gttgatttgg
     7381 aaggataccg ccaggggggc gtgagtctga cccccccggc gtaagagagg ctaattccgg
     7441 ctctggtagc tctttgcgag ggcatcgagg gcttcgtccg ccagacgacg atgatcgcct
     7501 tccgtcagtg cccgcccgac aactttctca gccaccatca tcgccaagtc ggtcgtttgc
     7561 gaacggatgt cctggatggc cttcctgcgc tcggtctcga tttcgcgagt cgcgtcaccc
     7621 ttgatgcgtt ccgcatccgc cgtcatgcgc tgttcgttct cttccatcag gcgttgggcg
     7681 cgttctttgg cctgtgc
//
LOCUS       DAORQN010000067         7692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_470_length_7692_cov_377.848108, whole genome shotgun sequence.
ACCESSION   DAORQN010000067 DAORQN010000000
VERSION     DAORQN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_7692_cov_377.848108"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PK614_05485"
     CDS             complement(<1..1031)
                     /locus_tag="PK614_05485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81696.1"
                     /translation="MKVLVISAAYPPMHAGEATNTYHLCRQLTERGIDIHVLTSVGNV
                     GTDDPRIRVYPIMQTWGWLELLRVRSFLKNCSPDAVFLMYIGLMYKFHPMVTFLPTLS
                     KGLFPNVPFVTRYESAFVGADPSKTGIVSRLFRKLMVQWAGGKDVAYSSGTLLRDSDA
                     VIALCERHRAMLLEEWPPVSQKVALIPPPPNLFIASNAGGMARERGRTRLGVTAGEFV
                     VTFFGYLYPIKGIETLLRAFASVSAQRPEARLLFIGGKVGLDVEGGVSYFDEMQALAK
                     ELHIDGLTTWTGAFKSEEEDASLLLHASDVCVLPFLEGVQLNNSSFASMVAHGLPIIV
                     TRGPLMDKA"
     gene            complement(1074..2144)
                     /locus_tag="PK614_05490"
     CDS             complement(1074..2144)
                     /locus_tag="PK614_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB81697.1"
                     /translation="MKRMLVTGSSGLIGSEVCAYFHGQGWSIHGVDNNTRATFFGPQG
                     DTRWNQRRLQADLKQFRHHELDIRDRKGALALIQELKPDVIVHTAAQPSHDLAAKIPF
                     DDFDTNAVGTLNLLEATRLHTPGAVFVHMSTNKVYGDAPNELPLVEQDKRWDYASPND
                     FDGITEHMRIDQSKHSLFGASKVAADVMVQEYGRYFGMKTCCLRGGCLTGPNHSGVEL
                     HGFLSYLVKCNLEGKKYSIFGYKGKQVRDNIHSLDVARFIHAFIENPRSGEVYNLGGG
                     RGNSCSIYEAFDMIEAISGKKMLYEYVDKNREGDHICYISNLKKMTTQYPGWGITKTL
                     KHIFEEIHQSWLTRAAAVNVPI"
     gene            complement(2193..3275)
                     /locus_tag="PK614_05495"
     CDS             complement(2193..3275)
                     /locus_tag="PK614_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB81698.1"
                     /translation="MARRFAMVGAFGVATVVSAAIMLGLGELTIRSIHLLRDGIPFFE
                     SVDGGRVGPISLDQELGWKATEHYQETLIERTNAGRPYSVRRSQKQYGFRQFGDLDSK
                     KMRLLVIGDSFTHATAVSDDRTYHALLAQLLDVEVFAYGAGGYGTLQELMILDRYIDI
                     IRPDVILWQYCANDFINNDNELERLSLVNNNGWVRPYLQKGQVQLLSPKESSLQVREW
                     INRRSRFLYFIVSRIDRLRAVRTRDTVEVAIESEGMRHPGFLRAVGVTDELMGRVRKK
                     VGNRSIMAFNCMRAEPYDQAFRDISARHGIAYWDDVASVVLEAHHRGEDVYAADGGHW
                     NERGHQLAAEAIASHFRAEMRTGTRP"
     gene            complement(3313..4149)
                     /locus_tag="PK614_05500"
     CDS             complement(3313..4149)
                     /locus_tag="PK614_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HRB81699.1"
                     /translation="MNTYQNKVAVVVPMHNRSELTPDEQISFQHLTHYLHAYDKYLVV
                     PNSLNIDLPGCSLKRFGDEYFGSVAANTRLLLSETFYRSFSEYQYILIYHLDALVFSD
                     QLEAWCDTGLDYIGPPWIHCADSPWVKEARVGNGGLSLRKIESFLKVFHSDVYWMEPE
                     EYWRERYAGMPAYVRMLNLPRRFAKRLSWLNNARLEMSRWHLRPDGTKNEDHFWSDRA
                     KHYVPEFRVASVEVGLRFAFEVAPRLCYDMNHRQLPFGCHAWPRYDRSFWEPYLITPH
                     AA"
     gene            complement(4292..5743)
                     /locus_tag="PK614_05505"
     CDS             complement(4292..5743)
                     /locus_tag="PK614_05505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB81700.1"
                     /translation="MAKLGRQLGKLANFVLLLGAALCAGVFAFLVWRHGMSLRYVVFV
                     GLAVILALGVRMPESLRINSVLVGVSTCVAVYLAELLLAYSGTAITSLGAQAWLSFPQ
                     DANVRVAAERMKTVQETHQKFDARSRLEVILDMRAHGVNAYPDVFPMVLFAPSSSDSI
                     QSVLTSQHEEFLPVAGMAATTTVFCNESGEYVVYESDEHGFHNPRGMWEHRPVELLAL
                     GDSYTHGVCVPSDKGFVAVVRGHHPNTMNLGVNSHGPLTSLATLKEYGPVLKPKLVLW
                     FYYEGNDLRDLDGWEKNSPLLMKYLSSSFSQHLFERQPEIDRSLRDYLDAAMAKATAP
                     ISLENILKLHHLRHAVQSFYERRPAEQGFPAELLEFLRHSGASAAPEDLQLFERILTE
                     AQATAKTWDGRVVFVYLPTWERYRLPELASKDRDNVLGIARRLKLHVMDMHEVFVTHP
                     DPLSLFPFRRYAHYNEAGHKLVGEEVLRQLGKL"
     gene            complement(5836..7044)
                     /locus_tag="PK614_05510"
     CDS             complement(5836..7044)
                     /locus_tag="PK614_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81701.1"
                     /translation="MLKRLWRYLQRYQLYLWGREVIAAVRRIWPQFRGGVVSLHAAGG
                     PHGHVLISYDNHGLLCKMRGQPIPTSHPQFLKTIIMAQTFVDLGYDVDVIHCENQRFI
                     PWKPYDIVVDTRLNLQRLQPYLPSTCIKILHCDTAQIVYQNAAEMGRMLAFQRRKGLT
                     VPPNRLETPHLGVEHADYLTTCGNEFTVNTYTYADKPIFRLPMVVQQMWPWPENKDFD
                     VSRHRFLWFGSRGMVHKGLDLVLEAFARMPECQLTIVGPVRNEPEFVNVYRKELFHTP
                     NITHVGWLDRSSEEFRIVLEQSLAHVFPSCSEAGAAAVVETMAAGVIPVVTYEASIDV
                     ENFGFLLEDASIETIMRQVREIAAMSVDELRRRAKKAWEAAHNNNTPEKFERSYRATI
                     EMILAKHGRR"
     gene            complement(7038..>7692)
                     /locus_tag="PK614_05515"
     CDS             complement(7038..>7692)
                     /locus_tag="PK614_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81702.1"
                     /translation="WTYQKVVLRESFHLSYPYVFEWHGRYYMIPETHQAESVRLYEAR
                     SFPTKWECTDTLLRGHRFNDNSIFYYHDRWWLFSETNPTLAHDTLRLYHAPDLRGPWQ
                     EHPRSPIIQENPHIARPAGRVVVIGDRILRFAQDCFPVYGTRVRAFEITELTPSTYRE
                     QPASRGSLFGPSWRLWTQGGMHHIDSHPLDSHQWIAAVDGWRQVGWPIPEGWQRATC"
BASE COUNT         1958 a         2234 c         1805 g         1695 t
ORIGIN      
        1 aatgccttgt ccatcagagg accccgtgtg acgatgatgg gaagcccgtg cgcaaccatc
       61 gaagcgaagg aactattgtt tagttgcacc ccctccagaa aaggcaacac acacacatct
      121 gacgcatgaa gcaatagcga ggcgtcctcc tcttctgact tgaacgcacc tgtccatgtt
      181 gtcaacccgt ctatgtgcaa ctccttggca agggcctgca tctcatcgaa ataggacaca
      241 ccaccttcaa cgtccagccc aacctttccg ccaatgaaca agagtcgcgc ttcagggcgt
      301 tgcgcggata cgctcgcgaa ggcccgcaac agggtttcaa ttcccttgat cgggtagaga
      361 tacccgaaaa acgtgacgac gaattcacct gccgtcactc ccaatcgtgt tcgtccgcgt
      421 tcgcgtgcca tgccccctgc attcgacgcg atgaacaagt tcggaggtgg agggattagc
      481 gccaccttct ggctgacggg aggccattct tcgagcaaca ttgcacggtg acgttcgcac
      541 aatgcgatga cagcatcact atcccgcaac agtgtccccg agctatacgc cacatccttt
      601 ccaccagccc attgaaccat cagcttcctg aacaaacgcg aaacaatgcc tgtcttcgac
      661 ggatcagccc ccacaaacgc actctcatac cgggttacaa acggaacgtt tggaaataac
      721 cccttggaaa gcgtgggcag aaaagtcacc atcggatgaa atttgtacat gagtccgatg
      781 tacatgagaa acacagcatc cggcgagcag ttttttaaaa aagaccgaac ccgaagcaac
      841 tcaagccagc cccatgtctg catgatggga tacacgcgaa tccgaggatc atcggtacca
      901 acattgccaa ccgacgtgag cacatgtatg tcaatgcccc gctcagtcaa ttgccggcac
      961 aagtggtagg tattggtagc ttcccctgca tgcatcggag gataggctgc tgaaattacc
     1021 aggaccttca tggcgtatgt atttcttcca acgctgacgt tagcacagat ttttcagatc
     1081 ggcacattca ccgcagcggc ccgcgtcagc caggactggt gaatttcctc gaagatgtgc
     1141 ttcaacgttt tcgtaatgcc ccagccggga tactgcgtcg tcattttttt caggttactg
     1201 atgtagcaaa tatgatcacc ttcgcgattc ttgtcgacat attcatacag catcttcttg
     1261 cccgaaatag cttcgatcat gtcgaacgcc tcataaatag agcagctgtt tccacgtccg
     1321 cctccgaggt tgtacacctc accgcttcgt gggttctcga tgaacgcatg gatgaatcgt
     1381 gcgacatcca gggaatgaat attgtctcga acctgttttc ctttatagcc gaagatgctg
     1441 tattttttgc cttctaaatt gcatttgacg agataactca aaaatccatg caactctacg
     1501 cccgaatgat tgggtccggt gaggcaacct ccccgcaaac aacaggtctt catgccgaag
     1561 tagcgcccat actcctgtac catgacatcc gcggccactt tcgaggcacc gaaaagcgaa
     1621 tgtttcgact gatcgatgcg catgtgctcc gtgatcccgt caaaatcgtt gggagacgca
     1681 taatcccaac gtttatcctg ctccacgaga ggcaattcat tcggggcatc gccgtacact
     1741 ttattcgtgg acatgtgtac aaacactgct cctggcgtgt gcaacctggt cgcttcgagg
     1801 aggttcagcg tacccaccgc attcgtatcg aaatcatcga acggaatctt ggccgcgaga
     1861 tcatgcgagg gctgtgctgc cgtatgcaca atcacatccg gcttgagctc ttgaatgagc
     1921 gccaatgcgc ccttgcggtc tcggatgtcg agctcatggt gccggaactg tttcagatca
     1981 gcctgcaacc ggcgttgatt ccaacgcgtg tcgccttgcg gtccaaaaaa cgtagcccga
     2041 gtgttgttgt cgactccatg gatcgaccat ccttgaccat gaaagtacgc gcacacttca
     2101 gaaccgatga gaccagaaga cccggtaaca agcattcgct tcatgatgtg cttactccct
     2161 ccctattctc aactcactgt actcgacatt catcaagggc gggtcccagt tctcatttca
     2221 gcgcgaaaat gactggcgat cgcctctgct gccaattgat gtccccgctc attccaatga
     2281 ccaccatccg cagcgtagac atcttcccca cgatgatggg cttcaagaac caccgacgca
     2341 acatcatccc aataggcgat accatgacgg gccgagatat cacgaaacgc ttgatcgtag
     2401 ggctccgctc gcatgcaatt gaatgccatg atcgatcgat ttccaacttt cttccggacc
     2461 ctccccatca actcatcggt cactcctacc gcacgaagaa atccgggatg ccgcatcccc
     2521 tccgattcaa tagcgacttc aaccgtgtca cgagtcctca ccgctcgcag ccggtctata
     2581 cggctcacaa taaaatagag gaacctgctc ctccggttga tccactctcg aacctgaagt
     2641 gaggactcct tcggtgacaa cagttgcacc tgccccttct gcaaataggg cctgacccac
     2701 ccattgttgt tcacaaggct caatcgctca agctcattat cattattgat aaagtcgttc
     2761 gcgcaatact gccagagaat gacgtctggt cgaataatat cgatataccg atcgagtatc
     2821 atgagttcct gcagtgtccc gtaaccacca gcaccgtacg caaatacttc aacgtccagc
     2881 agctgagcca ataaggcatg gtacgtacga tcatccgaaa ctgccgtggc atgagtaaac
     2941 gaatcgccaa tcaccagaag ccgcattttc ttagagtcca gatcgccgaa ctggcgaaat
     3001 ccgtactgtt tttgagagcg ccgaacggaa tagggccgac ctgcattggt tctctctatc
     3061 aatgtctcct ggtaatgttc ggtcgctttc cagccgagtt cctgatccag gctaatcggt
     3121 ccaacccttc caccgtcgac actttcgaaa aatggaattc cgtctctcag caaatggata
     3181 gaccgaatcg tcagttcgcc aagaccaagc atgatcgcag cagaaaccac cgtggccaca
     3241 ccaaacgccc cgaccatcgc aaaacgtctt gccatcgccg accccattct gtggccacat
     3301 tggccctgtc gatcaagccg cgtgcggcgt aatcagatag ggttcccaaa aagagcgatc
     3361 gtagcgtggc caggcatgac aaccaaatgg caactggcgg tgattcatgt cgtaacacaa
     3421 ccgaggcgcc acctcgaacg caaagcgtag gccgacctcg accgaggcga ctctgaactc
     3481 aggcacatag tgcttggcac gatcagacca aaaatggtct tcattcttgg tcccgtcagg
     3541 acggaggtgc caccgtgaca tctcaagacg tgcattgttc agccaggata gccgctttgc
     3601 aaaccgcctc ggcagattta gcattcgaac ataggccggc atccccgcat acctttcccg
     3661 ccaatattct tccggctcca tccaatagac gtccgagtgg aataccttga ggaaactctc
     3721 gatcttccgc agtgacaacc caccatttcc cacacgtgct tccttcaccc aggggctgtc
     3781 cgcacagtga atccatggcg gaccgatgta gtccagcccc gtatcgcacc aggcttctaa
     3841 ctgatccgaa aacaccaacg cgtcaagatg atagatcagt atgtattggt actcactgaa
     3901 ggaacggtaa aaggtttcgg acaataagag gcgtgtattc gcggcaacac tcccgaaata
     3961 ttcatcgcca aaacgcttga gcgaacaacc cggcaagtcg atgttgagtg agttcggcac
     4021 caccagatac ttgtcatacg catgcagata atgcgtgaga tgctgaaaag agatctgctc
     4081 atcaggagtg agctcgctcc gattatgcat cggcactacg acggctactt tgttttgata
     4141 tgtattcacc gtttcaaccc gtccatttca aaagcattct gtgctgatca acctcgagaa
     4201 cgcaaaaatc gaagccaccg caagccacga acggttatgt agcagaacac acttaccggc
     4261 acggcatttc gacacacaca atcggtagac gtcacagttt tcctaactgc ctgagcacct
     4321 cttcgcctac gagcttatgc ccggcttcat tgtagtgggc gtatcgtcga aacggaaaaa
     4381 gcgataaggg atccggatgg gtgacaaata cctcatgcat gtccattacg tgcagcttca
     4441 accgcctggc gatccccagc acgttgtcgc gatccttgct ggccaattca gggaggcgat
     4501 atcgttccca cgtgggcaga tagacgaaca cgacacgccc atcccacgtc tttgccgttg
     4561 cctgggcctc ggtcagaata cgctcgaaca gttgaaggtc ttccggagca gcggatgcgc
     4621 cggaatgacg aagaaactcc agcaactcag ccggaaaccc ctgttcggct gggcggcgct
     4681 cataaaatga ctgcacggca tgacggagat ggtgaagctt caggatgttc tcaagagaga
     4741 tcggagcggt cgcctttgcc attgcggcat caagatagtc cctcaacgaa cgatcgattt
     4801 caggttgccg ctcaaataag tgttgagaaa atgaggatga caaatacttc atcaagagcg
     4861 gagaattctt ctcccacccg tcgagatctc tgagatcatt gccttcataa taaaaccaca
     4921 gcaccagttt cggcttaaga accggaccat actctttaag cgtcgctaag ctcgtgagcg
     4981 gaccatggct atttacgccc aaattcatcg tattcggatg atgtccccgg accacggcca
     5041 caaatccttt atccgacggg acacacactc catgggtata ggaatcaccc agcgcaagaa
     5101 gttcgaccgg tctgtgctcc cacatgccac gcggattgtg aaagccgtgc tcatcgcttt
     5161 cgtatacgac atattccccg ctctcgttgc aaaataccgt cgtcgtagcg gccattccag
     5221 ccaccggcaa gaattcctca tgctggctag tcagtacgga ttggatactg tcactggaag
     5281 acggtgcaaa caggaccatt ggaaagacat ccggatacgc attgacgcca tgtgcccgca
     5341 tatccaggat cacctccaag cgcgaacgag catcgaattt ctggtgagtc tcctgcacag
     5401 tcttcattcg ttcagccgca acccgtacgt ttgcgtcctg agggaagctt aaccacgctt
     5461 gagcccccaa ggaggtaatt gctgtgccgg aataggcgag gagcaattca gccagatata
     5521 ccgcgacaca tgtggatacg ccgacaagca cactattgat cctgagggat tccggcattc
     5581 gtacgccgag cgccagtatc actgcaagcc ccacgaacac cacatagcgg agcgacatgc
     5641 catggcgcca taccaagaaa gcgaacacgc ccgcacagag cgcagcccct aacaaaagca
     5701 caaaatttgc caactttccc agctggcgcc caagcttagc cattcgtcaa tgcgctccct
     5761 acgccccaca gaggcttgac ggagaagaca tgatcctccg ccttccccga agcgaacatc
     5821 tttcggctgg tgctgctatc gtcttccatg ctttgccaaa atcatttcta tggttgcacg
     5881 ataggaacgc tcaaatttct ccggcgtgtt attgttgtgg gccgcctccc acgctttctt
     5941 agccctgcga cgcaattcat ccacagacat cgcagcaatc tcgcgcacct gacgcatgat
     6001 ggtttcgatc gaagcgtcct ccaaaagaaa gccaaaattc tccacatcaa ttgaagcttc
     6061 ataggtcacc acagggatta cccctgcagc catggtttct accacagccg ccgccccagc
     6121 ctcagaacaa gagggaaaga catgtgcaag ggattgctcc aagacaattc gaaactcctc
     6181 actggaccta tccagccaac cgacatgtgt gatattcggc gtgtggaata attctttccg
     6241 ataaacattc acaaactctg gctcattgcg caccggccct acaatggtaa gctggcattc
     6301 cggcattctc gcgaaagcct ccaacaccaa gtcgagtccc ttgtgaacca tccctcgact
     6361 tccaaaccaa agaaaccggt ggcgggacac atcgaagtct ttgttctccg gccaaggcca
     6421 catctgctgg acaaccatgg gtaaccgaaa aatcggctta tccgcatagg tatatgtgtt
     6481 caccgtaaac tcattgccgc aggtcgtgag gtaatccgcg tgttcaaccc ctaaatgcgg
     6541 tgtttccaat cgattcggcg gaaccgtgag ccccttccgc cgttgaaacg ccaacattct
     6601 gcccatttca gccgcgtttt ggtagacaat ctgagccgta tcacagtgca gaatcttgat
     6661 gcaggtagaa ggcaaataag gttgtagtcg ctgcaaattc agacgtgtat caaccacaat
     6721 atcgtacggc ttccagggaa tgaatctctg attctcacag tgaatcacat ctacgtcata
     6781 gcccaaatcg acaaaggtct gagccatgat gatagtcttc agaaactgag ggtggctggt
     6841 gggaatgggt tgccctcgca ttttacagag cagcccatga ttgtcgtaag aaatcaaaac
     6901 gtggccgtgt ggaccccctg cggcgtgcaa ggaaacaacg cccccacgaa actgaggcca
     6961 gatgcggcga accgcagcaa tcacctctcg tccccataga tacagttggt accgttgcag
     7021 atatcgccac aaccgtttca acacgtcgcc ctctgccacc cttcaggaat gggccatcca
     7081 acttgtcgcc acccatccac tgcggcaatc cattgatggg aatctagagg atgggaatcg
     7141 atatgatgca tcccgccttg ggtccagagc ctccagctcg ggccgaataa tgaaccacgc
     7201 gaagcgggct gctcacggta ggttgaagga gtcagctccg taatttcgaa cgcccgtacc
     7261 ctcgtcccat agaccggaaa acagtcctgc gcaaacctga gaatgcgatc gccgataacc
     7321 acgactcgtc ctgccggcct tgcgatatgg gggttctctt gaatgatcgg agatcgagga
     7381 tgctcctgcc agggacctcg aaggtcagga gcatgataga ggcgaagcgt atcgtgtgcc
     7441 aaagttgggt tggtttcact gaataaccac catcgatcat gataataaaa gattgaatta
     7501 tcattgaaac gatggccccg gagcaacgtg tcggtacact cccactttgt tggaaacgac
     7561 cgagcttcgt agagacgcac actctccgcc tggtgggttt ccggaatcat ataatatcgg
     7621 ccgtgccact cgaaaacgta gggataggac aggtgaaatg attcccgtaa tacaactttc
     7681 tgataagtcc ag
//
LOCUS       DAORQN010000068         7594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_474_length_7594_cov_10.992572, whole genome shotgun sequence.
ACCESSION   DAORQN010000068 DAORQN010000000
VERSION     DAORQN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_7594_cov_10.992572"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1668)
                     /locus_tag="PK614_05520"
     CDS             complement(13..1668)
                     /locus_tag="PK614_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRB81703.1"
                     /translation="MAEAGFISIADREQAVAEKLNFRRPGSEHLGPYFVEYIRQLLVA
                     KFGEAMVYKGGLEVFTTLNADMQKAAETAVFNGLREVDKRQGWRGPLRTVDLATLEVP
                     APDPSVKLAEGDIMEGVVTKIAKDHVLVQIGGSTGRLAFDDMAWATKYLKGKDPTKDA
                     TPIKNLKQLLALGDVIEVGVKKLEKETVHLRLEQTPLVEGALVAVDPKTGAIRAMVGG
                     YDFARSEYNRAVLARRQPGSSFKPIIYATAMNQGMSPATVVLDAPVVYEQEEEDKTWK
                     PENYGKRFHGIVSLREALIHSHNLATVRLLDKVGIRNVIDFSRTVGIVSPLAADLSLA
                     LGSSGVGLMELVSVYGVFANQGMRVEPYAVASVQDSGGRTLDQAAIQPRQVVSRETAY
                     LITNMMEDVIQRGTGMAAKAVIDRPVAGKTGTTNDFTDAWFIGSTPNLATGAWVGFDD
                     RRPLGETESGAHAALPIWIAFMKDALKQLPVVPFEIPDGVMFVKVDPTTALLTDQDEQ
                     HGTVELFTKGTEPTKSAGSKIDPTEFYKLDQLQDSAPPVSLEP"
     gene            complement(1674..2396)
                     /locus_tag="PK614_05525"
     CDS             complement(1674..2396)
                     /locus_tag="PK614_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRB81704.1"
                     /translation="MSELDHLLDKPEKPRRRWRWWKIAVIGLLLAIVLGGGGAAGILW
                     YFSQDLPSLDPLQNYQPSLVTRVYSDDRQVIGQFFIERRFLKPIQEMPKSLTQAVIAT
                     EDTRFFEHPGLDIVGILRAAWTNIRHGGRKVEGASTITQQLARSLFLSAERTFDRKVR
                     ELILAYKMELVLTKEQILEMYLNQIYFGQGAYGVAAASQTYFGKELSKLTLAESAFLA
                     GLPKSPSHYSPFKAYDRAKSMC"
     gene            2620..4503
                     /gene="typA"
                     /locus_tag="PK614_05530"
     CDS             2620..4503
                     /gene="typA"
                     /locus_tag="PK614_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRB81705.1"
                     /translation="MNSSAPSSTTSGLHAPQGRRTDIRNIAIIAHVDHGKTTLVDAVL
                     RQTHVHRKIDDMGERIMDSMDQERERGITIRAKNASVTYKGVKINIVDTPGHADFGGE
                     VERTLRMVDGVLILVDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVN
                     RTFDLFVHLGATDEQLDFPIVYTSAIKGVATLDLKQPGTEISPLLETILDKIPAPAIN
                     ADAPFQLLVLALAQDSYKGKMGIGKIQSGSIARRQNVVTLTKDGGQVPGKISDLAVFS
                     GLERTDVETAEAGEIVALSGLEEVNIGDTIADPTNPVALPRVTIDEPTVQMTFSVNNS
                     PFAGRDGKYLTSRHLRERLFKELETNVSLRVQETDSADRFLVAGRGELHLGVLIEQMR
                     REGYELQISQPEVILHREGDTVTEPFEELTIQVPSEYQGPVIEEIGKRRGELRHMKLV
                     HSEGTASEMHIEYHIPTRGIIGLKNILLAKTRGTIILHHVFSGYAPADEKALLVAPHG
                     SLVAFEAGTSTAYALFMTQERGELFIGATVEVYQGMVVGQNSRDEDLDVNVCKQKQLS
                     NMRAAGTDEALVLTPPREMSLEFAMEYIGQDELVEVTPKYLRLRKRLLNPDDRRKAKK
                     SSK"
     gene            4540..5295
                     /locus_tag="PK614_05535"
     CDS             4540..5295
                     /locus_tag="PK614_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81706.1"
                     /translation="MKIRKKASKPAPAKPPLYLVGYRGAPPSLDELKIWYDLQYGGPL
                     ICLEHDAGGRVTANHGPWQAHLLTSIPPSEAAQWQPVLSWDHRQLGAVSPAATAPSTI
                     ADTVLVAARLARGLTLLTQGTAFDVLCHEYLNPSDWNDRSLNVFLPRDHVTVQQSEST
                     DESSEWFHTLGLNKFGLDELEMIQPRGLPDTETIALLKSAADRVLRSGQNQKVGSSLD
                     LPALAHTIRFIKHRTAAPTGRMMAFRQITPDIL"
     gene            5457..5927
                     /locus_tag="PK614_05540"
     CDS             5457..5927
                     /locus_tag="PK614_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRB81707.1"
                     /translation="MSDVAAEIKVGDTAPDFTLKDQDQKDVKLSDFRGKSNVVLAFYP
                     LDWSPVCQGENKCLTDDFPKFQSAHAELFGISCDSFFSHKAWADSLDLKHRLLSDFNR
                     EVVKKYGLYFEPLNCGKRATVIVDKNGKVAYVKVQEIKVAREDKDILAALAKLS"
     gene            6005..6337
                     /locus_tag="PK614_05545"
     CDS             6005..6337
                     /locus_tag="PK614_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRB81708.1"
                     /translation="MTGTVQDVRDENYKEFTESTGAVVAYGLATCEPCKQYDPILEET
                     AAKFPTIKIGKAKMHVPGRCREIKKAHTFETYPTTHFFAQGKLLLTREGVVDPAELAA
                     LISDHLLK"
     gene            6694..7386
                     /locus_tag="PK614_05550"
     CDS             6694..7386
                     /locus_tag="PK614_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB81709.1"
                     /translation="MALVPAGEFAMGSDRGQEDEQPVHRVSVNTFYLDVYETTVSHYA
                     EFLASQRPDAPFKWNEAAAGAHDKRPVVGVNWYDARDYCRWAGKRLPTEAEWEIAARG
                     TEGRIYPWGSTHPTKGHANAGETRWRGYDSLSNVGRFELGKTPEGVYDLAGNLWEWVA
                     DWYDPTYYQFSARDNPKGPSAGPLRALRGGAWNNDSKAIRSSNRAGYAPDARRNDVGF
                     RCAQDGPSSERR"
     gene            complement(7383..>7594)
                     /locus_tag="PK614_05555"
     CDS             complement(7383..>7594)
                     /locus_tag="PK614_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81710.1"
                     /translation="PWAYRCFHEKTSASGRGRQDLVMDESRGLDRDKSRRVVFKIRLR
                     PTAQQSEMKQALRTIEEPNFSSRLF"
BASE COUNT         1694 a         2345 c         2090 g         1465 t
ORIGIN      
        1 cccggcgtcg ggctacggct cgagagaaac tggtggggca ctgtcctgca attggtccag
       61 cttatagaac tcggtgggat cgatcttgga tcccgcactc ttggttggct cagtcccttt
      121 ggtaaagagc tccacggtgc cgtgctgctc gtcctgatca gtgaggagag ccgtcgtggg
      181 atccaccttc acgaacatca ccccgtctgg aatttcaaac ggcacaaccg gcagttgctt
      241 gagcgcatct ttcatgaacg cgatccagat cggtaaggcc gcatgcgcgc cggattccgt
      301 ttcacctaag gggcgacgat catcgaaacc cacccaggcc ccggtcgcga gattgggcgt
      361 cgaaccgatg aaccaggcat cggtgaagtc attggtcgtc ccagttttcc ctgccaccgg
      421 ccggtcgatg acagccttgg ccgccatgcc tgttccgcgt tgaatgacat cttccatcat
      481 attcgtaata agatatgccg tttcgcggga cacgacctga cgcggctgaa tggccgcctg
      541 atccaacgtg cgaccgccac tgtcctgcac gcttgccacc gcataaggct ccacccgcat
      601 gccctggttg gcaaatacac cgtacactga cactaactcc atgagcccca cgccggacga
      661 acccaacgcc agcgaaagat cggcagcgag cggactcaca ataccgaccg tccgcgagaa
      721 atcgatcacg ttgcgaatgc ccactttatc caggagacgc acggtcgcga gattgtgaga
      781 atgaatcagc gcttcccgca aactcacaat gccgtggaac cgcttgccgt agttttcagg
      841 cttccacgtc ttatcttctt cctcctgttc gtacaccacc ggggcatcca gcaccaccgt
      901 agccggactc ataccttggt tcatcgccgt ggcgtagatg atcggcttga acgaggaccc
      961 gggctgccgg cgcgcgagca ccgcacgatt gtattcgctc cgcgcaaaat catacccgcc
     1021 gaccatggcg cggatcgcgc ctgttttggg atcgacggcc accaacgcgc cttcgaccaa
     1081 cggagtctgt tccaatcgca gatgcaccgt ctccttctca agtttcttca ctccgacctc
     1141 gatcacatca ccgagtgcca acagttgctt gaggtttttg atcggagtcg catccttcgt
     1201 gggatccttg cccttgagat acttggttgc ccaggccata tcgtcgaagg ccagccgacc
     1261 ggtgctaccg ccgatttgga ccagcacgtg gtctttggcg atcttcgtca ccaccccttc
     1321 catgatgtcc ccctccgcca gcttcacaga gggatcgggt gccggcacct ccagggtcgc
     1381 cagatcgacg gtgcggagcg gaccgcgcca accctggcgc ttatcgacct cgcggagtcc
     1441 gttgaacaca gccgtttcag ccgctttctg catatcggca ttcagcgtgg tgaacacttc
     1501 cagtccaccc ttgtacacca tcgcttcacc aaacttggcc accagcaatt gccggatata
     1561 ctccacgaaa tagggcccga gatgttcact accaggacgg cggaagttca gcttttcggc
     1621 tacagcttgt tcacgatcgg cgatactgat aaaacccgcc tcggccatcc ggctcaacac
     1681 atgctcttgg cccggtcata ggccttaaag ggcgagtagt ggctggggga tttgggaagg
     1741 ccggcgagaa acgccgattc agccagcgtg agctttgaca gttctttccc gaaataggtc
     1801 tgactggccg ccgccacgcc ataggcgccc tggccgaaat agatctggtt caaatacatc
     1861 tccagaatct gttccttggt cagcaccagt tccatcttgt aggccagaat gagttcccgg
     1921 actttgcgat cgaacgttcg ttctgccgaa agaaacagag accgcgccag ctgctgggta
     1981 atggtactgg cgccttcgac tttccggccg ccatggcgaa tattggtcca ggccgctcgg
     2041 agaatcccga cgatgtccaa gcccgggtgc tcaaaaaatc tcgtgtcttc cgtggcgatg
     2101 acggcttggg tcaggctctt cggcatctcc tgaataggct tgaggaaccg tcgttcgatg
     2161 aaaaactggc cgatcacttg ccggtcgtcg gaatagacgc gcgtcaccag gctcggctga
     2221 tagttctgca agggatccag cgaggggagg tcttgggaga aataccaaag aatgccggcc
     2281 gccccgccac caccgagcac aatggccagc agcaggccga tgacggcaat cttccaccat
     2341 cgccagcgac ggcgaggctt ctccggctta tcgagtaagt gatcgagttc ggacatgaag
     2401 ttggatggtt gcgaggaaag gggtcaaccc gcgacaactc caccatacaa tgcacacccg
     2461 acaaactcaa gcaaggttcg aatcatcgtc cgaagaagtt tccgcggaac attcgacaat
     2521 cagcttgcgg gaagacgttt gaatcgggta tagtccatag gattagacgc cttcatagga
     2581 aggcgtcttt tttttggcac tttcacggca ttggagatta tgaacagctc agcgccctca
     2641 tccaccacat cgggtctgca cgcgccgcaa ggtcgtcgga ccgacatccg caacattgcc
     2701 atcatcgcgc acgtcgacca cgggaaaacg actctcgtcg acgccgtgct gcgccagacc
     2761 cacgtgcatc gcaagatcga tgacatgggt gagcgcatca tggactcaat ggatcaggag
     2821 cgcgagcgtg gcatcacgat ccgcgcaaaa aacgccagcg tgacctataa aggtgtgaag
     2881 attaacatcg tcgacacgcc gggccacgca gatttcggcg gcgaagtgga gcggacactc
     2941 cgcatggtgg atggcgtgct gatcctcgtc gatgccaaag aaggccccat gccgcagacc
     3001 acattcgtct tgcgcaaagc gttggccctc ggacataaag ccatcgtcgt catcaacaaa
     3061 atcgatcgcc ccgatgcggt catcgatgac gtcgtcaatc gcaccttcga tctcttcgtg
     3121 catctgggag ccaccgacga acagctggac tttcctatcg tctatacctc cgccatcaag
     3181 ggggttgcga ccctcgatct caagcagcca gggacggaga tctccccgct cctggagacg
     3241 attctcgaca agattccggc accggccatc aacgcggacg ccccgttcca gctcctcgtg
     3301 ctggccctgg cccaggattc ctataaaggg aaaatgggga tcggaaagat ccaatccggc
     3361 tccatcgccc gccgccaaaa cgtggtcact ctgaccaagg acggcggcca ggtccccggc
     3421 aagatttccg atcttgcggt gttttcaggc cttgagcgca ccgatgtcga aacggctgaa
     3481 gcgggtgaaa ttgtcgctct ctccggcctg gaagaagtga acatcggtga cacgattgcc
     3541 gatccgacca atccggtcgc gctcccgcgc gtgacgatcg acgaaccgac cgtgcagatg
     3601 acgttttcgg tcaacaacag cccgttcgcc ggccgtgacg gaaaatacct gacctcgcgc
     3661 cacctgcgtg aacgattgtt caaggagctg gaaaccaacg tctccctgcg cgtgcaggaa
     3721 acagacagcg ccgaccggtt tctcgtggcc ggccgtggcg aacttcatct cggcgtgctg
     3781 atcgaacaga tgcgtcgtga aggatatgag ctgcagatct ctcagcccga ggtcatcctg
     3841 caccgagaag gcgatacagt cacggaaccc tttgaagagc tgaccattca ggttccttct
     3901 gaatatcagg ggccggtgat tgaagaaatc ggcaaacgac gcggcgaact ccgtcacatg
     3961 aagctcgtgc attccgaagg caccgccagc gaaatgcaca ttgagtatca catcccgacg
     4021 cgcggcatta tcggcctaaa aaacatcctg ctggcgaaga cccgcggtac catcattctg
     4081 caccacgtgt tttccggtta tgccccggcg gatgaaaagg cgcttctcgt ggcaccgcat
     4141 ggttcgctgg tagccttcga agccggaacc agcacggcct atgcgctctt catgactcaa
     4201 gaacgcggcg agttgtttat cggcgctacg gtcgaagtct accagggcat ggtcgtcggc
     4261 cagaacagcc gcgacgagga cctggacgtc aacgtgtgca agcagaaaca attgagcaac
     4321 atgcgcgcag ccggcacgga cgaggcctta gtgctcacac caccgcgtga aatgtcattg
     4381 gaattcgcga tggaatacat cggccaggat gaattggtcg aggtgacgcc gaaatatctc
     4441 cgcctccgca agcggctact gaatccggac gaccgccgca aagcgaagaa aagctccaaa
     4501 taactccgca ggcgcccggc gccttgctga gcggccggca tgaagattcg gaaaaaagcc
     4561 tcgaagcctg cacccgcgaa gcctccactt tacctggtcg gctatcgcgg ggcgccgccc
     4621 tctctcgacg aactgaaaat ctggtacgac ctccagtacg gggggccgct gatctgcctc
     4681 gagcatgacg caggaggccg cgtgactgcc aaccacggtc catggcaggc gcacctcctg
     4741 accagcattc ccccatccga ggccgcacag tggcagcccg tcttatcctg ggaccaccgg
     4801 caactcggag ccgtctcacc agccgccacc gccccaagca ccattgcaga tacggtgctc
     4861 gtcgctgccc ggctcgcccg cggcctgaca ctgctcaccc agggcacggc cttcgatgtc
     4921 ctgtgccacg agtacctaaa tccgtcggat tggaatgaca gatcattgaa tgtcttttta
     4981 ccccgcgacc atgtcaccgt ccagcagagc gagagtaccg atgaatcatc cgaatggttc
     5041 catacattgg gattgaacaa gttcgggctg gacgaactgg aaatgatcca gcctcgaggc
     5101 cttcctgata ccgagaccat cgccctcctc aagtccgcgg ccgacagagt cttacggtcc
     5161 ggacagaatc aaaaagtagg aagctcgttg gaccttccgg ccctcgctca caccatccga
     5221 ttcatcaaac atcgcaccgc agcgccgacc ggccgcatga tggcattccg acaaatcacc
     5281 cctgacattc tctgacccgc cacctcagaa agtccgcaaa gcatcacgaa atcaagtgat
     5341 tgtgactgta cccgcttccc cctaggcctt ctggccccgt tgacaaggtt tttccaggcg
     5401 tggtaccaca aaaaggtttt gcgtagtgct gtaggtctaa cctaggagga ttctcaatga
     5461 gtgatgtggc tgcagaaatt aaggtcggcg ataccgcgcc ggatttcaca ttaaaggatc
     5521 aggaccagaa ggacgtgaag ttgagcgatt tcagagggaa gagcaacgtt gtactcgctt
     5581 tttacccgct cgactggagc ccggtctgtc agggtgagaa caagtgcctg accgatgact
     5641 tcccgaagtt ccagagcgcc cacgccgaac tgttcggcat cagctgcgac agcttcttct
     5701 cccacaaagc ctgggcggat tcgctggacc tcaagcaccg cctcctctcc gacttcaacc
     5761 gcgaagtcgt caagaagtac ggactgtatt ttgagccgct gaattgcggg aagcgcgcca
     5821 cggtcatcgt cgataagaac ggcaaagtcg cctacgtaaa agttcaggaa atcaaggtcg
     5881 cgcgggaaga caaggacatt ctcgccgccc tcgcgaagct gagctaagcg ccacgaggga
     5941 ccgagggggg aggggtcacc acgacgcccg cccctcggtc atccctcgta gctgaggaga
     6001 gagcatgacc ggcacagttc aggacgtccg agacgagaat tacaaagaat tcaccgagag
     6061 cacaggtgcg gtggtcgcct acggcttggc gacctgcgaa ccctgcaagc aatacgatcc
     6121 cattctcgaa gaaaccgctg cgaagtttcc caccatcaaa atcggcaagg ccaaaatgca
     6181 cgtaccaggc cgatgccgag agatcaagaa agcgcatacg tttgagacct accccaccac
     6241 gcatttcttc gcgcagggca aactgctgct gactcgtgaa ggggtggtcg acccggccga
     6301 gctggcggca ttaatctccg accatctgct gaagtaacag cctccatctc cggtagccct
     6361 caccgcaccc actgcagccg tgctgcaact tgtccgtcgc taaggacgcg caccctacac
     6421 ggtcctcccc cacacttttt cgcacgaacg ggatacaatg tcggggtgag accagaacta
     6481 ccgaccgtct cgcgatcgat cacccacgga acaatgcgac cattcgtacc gttcacctgt
     6541 gccatcgcgc tactttcttg tctcccattc gcaggggctg ctgaagcgaa ggacatctcg
     6601 ccacacccgt cacattcaat cgtaggcaac ccttcaaacc ccgcctccgt cgaacttcgc
     6661 gccaagttca ccggcacgcc acgtggtgcg ccaatggcgc tcgtccctgc cggggaattt
     6721 gccatgggca gcgaccgcgg tcaggaggac gaacagccgg tccatcgcgt ttcggtcaac
     6781 accttttatc ttgatgtgta cgaaaccacc gtctctcatt atgccgagtt cctggcctcc
     6841 cagagaccgg acgcgccgtt caagtggaat gaggcggccg ccggcgctca cgacaagagg
     6901 cctgtggtcg gcgtcaattg gtacgatgcg cgtgactatt gccgatgggc ggggaaacga
     6961 ttgccgacag aggcggagtg ggagatcgca gctcgcggca ccgagggacg gatctaccca
     7021 tggggaagta cgcacccgac caaaggacat gcgaatgcag gtgagaccag atggcgagga
     7081 tacgacagcc tgagcaatgt tggacgcttc gaactgggca aaacaccgga gggggtgtat
     7141 gacctggcgg ggaatctctg ggaatgggtc gccgactggt atgatccgac ctactaccaa
     7201 ttcagcgcac gagacaatcc gaaaggacct tccgcggggc cacttcgggc attgcgcgga
     7261 ggcgcctgga acaacgattc caaggccatc aggtcgtcaa atcgcgccgg atatgcgcca
     7321 gatgcgcgac ggaatgatgt cggatttcgt tgcgcgcagg acgggccgtc ctcggaacgg
     7381 cgttagaaca ggcgggagct gaagttcggc tcttcaatcg tccgcaaggc ttgtttcatc
     7441 tcactctgct gagccgtggg ccgtagacgg attttaaata ccacccgccg gctcttatct
     7501 cgatcgaggc cacgcgactc atccatgacg agatcttgcc gacctcgccc actggcagac
     7561 gtcttttcgt ggaagcagcg ataggcccag ggcg
//
LOCUS       DAORQN010000069         7504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_479_length_7504_cov_19.130487, whole genome shotgun sequence.
ACCESSION   DAORQN010000069 DAORQN010000000
VERSION     DAORQN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_7504_cov_19.130487"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="PK614_05560"
     CDS             <1..1437
                     /locus_tag="PK614_05560"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRB81711.1"
                     /translation="LKSIFGETSPSCKQFAQIQWTYRGKMIVSDDEILDLRIARERYD
                     KPVFQNALNEGLGILLAAKKELLGSGLANTNVSPIALSQPNSRRVEPMDIMLSWSGKQ
                     SNAIATALYNWLPSVIPGVQLWMSSEDIAKGKRWFQQLLHPLSEFKACITCMTPQNVT
                     SSWIFFEAGAFASKKDDAHLFTFLVGVQDSDLSNSPLAQYQWTHNTKDDTWRLIRDLN
                     KALAKGHDERLLKSHFDFHWNALKRTMDEVESQFQSIEKPRELTAVAISNSSEDLSLT
                     PQEMTLLMKLESSREGVVHRIKSDLGVHFMVDGTTFNMNCPPKENAAWQNGFEKLRRM
                     KIIEDRERDGGRYHLSSKGFEVAEQIRGQLGNQVKAQLRNKETPNLSPEAARLLFEAC
                     KDQHGVILKSSTLQGTSFSTNREEFGDSTNPRSVAKWKAAAEELATNGFVDSSKPGHS
                     MTFRVNAAGYSAAEKISSESVQGVDISK"
     gene            2174..3037
                     /locus_tag="PK614_05565"
     CDS             2174..3037
                     /locus_tag="PK614_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81712.1"
                     /translation="MLALCILLLFTQADGFDPGPNPNKDITKPVVDFESNSVKQKLKL
                     ANSGEIAVLEALAKRVKDWQSFKIENLSRPIMGKPVHATEILIRYIDVFDMEQLRPWL
                     VKKAEFDGFDKNIKHKIKNLNAAKHEYALMDTDSRTYIKGIYNWIEKGSRVGDLPSQL
                     KSIAASHKNLFDPDELATWSKEAASNKEKMKAEISKELAAEKVREQEAQEKKKNDDAV
                     AKVMEDKRKAQQKIKDDEDKARVLLKAAKLFMEDNANEFAERRLKEIITKYPNTKSVD
                     EAKQLLEKMKK"
     gene            3057..3278
                     /locus_tag="PK614_05570"
     CDS             3057..3278
                     /locus_tag="PK614_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81713.1"
                     /translation="MAKYVVNTKVNGKLVSQDHQTKPAALKHLDSIWQTTNAMEAEIH
                     YWATYPKLPAPNKKPKVIERRERTKQAFP"
     gene            3371..3964
                     /locus_tag="PK614_05575"
     CDS             3371..3964
                     /locus_tag="PK614_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRB81714.1"
                     /translation="MANKRLGTAATELKLPTKSVKGEKKPKWQAKTGNKKSRDAEVVE
                     TPGVVVETTVQGEITLPPEPTEVPVVATTPEGTGVEPATVDEAATTTAPEPTSPTEEP
                     SDETTHKKPRKLKVKADGPPRKLSMVAAALRVLQERKVSMTCPELIDVMATEGLWVSP
                     GGKTPANTLYAAISRIIKEQGKASAFRKGERGRFEAV"
     gene            complement(4020..4289)
                     /locus_tag="PK614_05580"
     CDS             complement(4020..4289)
                     /locus_tag="PK614_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81715.1"
                     /translation="MNEVTDGNKQIVREIILGYFELFETDSFTRQHIYNVVVADRDMP
                     KIDDQVSIVTQEFDRLLLETKIQVVSGSGLQERFKLKRIPKQRFR"
     gene            4605..5981
                     /locus_tag="PK614_05585"
     CDS             4605..5981
                     /locus_tag="PK614_05585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRB81716.1"
                     /translation="MDPTRADFMQKELNGKMVAGWVVGRVIGNGASAVVFEATRDGLT
                     AALKVLDRELVAKVSEAQQLARVNRERELIGKEHPNLVKILDGGKCPESGLLFIVMEC
                     LTWQELRKVIGEIPRARIAPIIEQLASAAEFLEDLGIAHRDIKPANILISPDFKVIKL
                     MDLGIIKPIGEEGLTDSEKKPFIGTHQYSPPEFIHRLEENDKEGWRAVTFYQIGAVLH
                     DLIMRRPIFQNAAHRTADLVNAIESDRPVIDAPDVPADLRSLAAMCLSKDSEKRRRVL
                     KWSSFHQPQTTPTVNTVRERLRELQTSLTGPSEAVTTKGVVDELVLDGLVNCIKDSIR
                     QETIKHSDRLPPVIFADVERSAATMTFSATYGPSPTHLLSAMLYIRYKVGLIDSMMRV
                     LDICACATLSSGEPSNECFSDYSSVFQGAYIEELCTPRVLTALYMAVTNAATVQAQVN
                     KCYAINCN"
     gene            5994..7148
                     /locus_tag="PK614_05590"
     CDS             5994..7148
                     /locus_tag="PK614_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB81717.1"
                     /translation="MSDTWWVKSTQLSPEQRDIVGITDLKNRLVLGPPGSGKTNILLL
                     RAKYLIADSKPDVQIIVFTKALRRFVASGREHYGVAADKIITSTKWSHRLLRDLGVNP
                     DPPEDFSDARKYYSDMISNLVEQKKIDKLHDAILLDEAQDYTFAEIQLFAKVAKHLFA
                     VADTRQQIYSATDDPVEALKKLVEHTHELKSHYRCGKKICELADALRKYTTLHNPLIN
                     SMMYDEEEYPSSVTCDSGSLRAQCDLIAQRLADQMRVYPGQFLGIACPRRDGVERVMY
                     LLQRYPEITRHVCRVKEDAESFDGQKSICLGTIHDMKGLELRTLHIPEAETLRDMPHP
                     RNLAFTAVTRAKTSLSFYHEMEMIGYLDTAIRSILPTGEKPSVESLFPEG"
     gene            7152..>7504
                     /locus_tag="PK614_05595"
     CDS             7152..>7504
                     /locus_tag="PK614_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81718.1"
                     /translation="MWAGVYNRKDMLDVLLGTKGNRLATRADFVDVLPNSRGQDQSVV
                     VWSANPAEQFQTPSLVIVEDGGVREFLAWVTSYVQELFPFTSFCRIADRSLIEMYLAK
                     QNGEHCAVDDGVMAG"
BASE COUNT         2128 a         1720 c         1944 g         1712 t
ORIGIN      
        1 ctgaagtcta tttttggtga gacttctccc tcttgtaaac aatttgctca aatccaatgg
       61 acatatcgcg gaaagatgat tgtctcggac gatgaaatat tggacttacg tatcgcaaga
      121 gagcggtatg ataaacctgt atttcagaac gctctcaatg aaggtcttgg cattctcttg
      181 gcagcaaaaa aagagttgct aggaagtggt ttagctaaca caaacgtaag ccccatagca
      241 ctatcgcaac caaacagtag gcgagtcgaa ccaatggaca tcatgttgag ttggtccggg
      301 aaacaaagca acgctatcgc cacggccctc tacaattggc ttccaagcgt cattccaggt
      361 gttcaactat ggatgtcgag cgaagatatt gcgaagggta aacggtggtt tcaacaactg
      421 ctccatccgt tgagtgagtt caaggcgtgc ataacttgta tgactcccca gaatgtgact
      481 tcaagctgga ttttctttga agcaggagcg ttcgcatcta aaaaggatga cgctcaccta
      541 tttaccttct tggttggtgt acaagatagc gacttgagta atagccctct tgctcagtac
      601 caatggacac ataacaccaa agatgacact tggaggctga ttcgtgacct aaataaggct
      661 ctggcaaaag gacacgacga gcgcttgctg aaatcccatt tcgactttca ctggaatgct
      721 ctcaagagaa ccatggatga agtagaatcg caattccaat caattgaaaa gccacgagaa
      781 cttactgctg ttgcaatttc aaacagctcc gaagatcttt cattgacacc gcaagaaatg
      841 actctcttga tgaagctcga atcttcgcgt gagggggttg tgcatcgcat taaaagcgat
      901 ttgggagtac atttcatggt tgacggcaca acgttcaaca tgaattgtcc cccaaaagaa
      961 aatgctgcct ggcaaaatgg tttcgagaag ctacgccgaa tgaaaattat cgaagataga
     1021 gagcgcgatg ggggacggta ccacctctcg tcaaaaggtt ttgaagttgc cgagcagata
     1081 agaggacaac ttggcaacca agtgaaggct caactcagga acaaagagac acccaattta
     1141 tcccccgaag ccgcacggct cctttttgaa gcatgtaagg atcagcatgg ggttatacta
     1201 aaaagctcaa ctttgcaagg caccagtttc tcgacaaata gggaagagtt cggcgattcg
     1261 accaacccta gatcagttgc taaatggaaa gcagcggctg aggaactcgc cactaatgga
     1321 ttcgttgata gttccaaacc agggcacagt atgaccttta gagtgaatgc agcagggtac
     1381 tcagctgctg aaaaaatctc gtctgaatct gttcaaggtg tagatattag taagtagaaa
     1441 acaacagtgt acagcacacg ggcttagtat catttcaacg aggatgacct taggccatcg
     1501 cacatagcat acaatcctcg cctaattcct ggttcgtgcc agctgcgaga aggaaggagt
     1561 cagggttctg aaaggggcag tgcggtaagt tcgaggcgtg ctaacaagta tggcacaaca
     1621 acgaaacagc ccggctgaga ggctgggtcg gtgtcattgc ttctatttag ctgttgtaga
     1681 cagttccaca caactcgccc ctgtctgctg catgttgctg agattaactt catgctgggt
     1741 gacctgatgg cgactgaagg cttgtgggta tcgcctggcg gtaaaacgcc tgcgaatacg
     1801 ctctacgcag caatcagcag aatcatcaag gaacagggga aggagtcagc gttcaggaag
     1861 gcggagaggg ggaagtttga ggcggtttag aggtggtgga gcgcgaacga ctccggctgg
     1921 tcggctgggg tgaatctcgt tggcgtttca gaactatcgt tcttatgtgc acatcaaaca
     1981 aactctgaag tccgagcttc aagtacttca ttaagtctta ctcttgaatc agtcagttca
     2041 cttgggagat cgttttccga gcgaatcggc tcaccagaag ggattggtat ttctgggtgg
     2101 gggtagctat gaaaaaaatg gaactcagct ttggattaca tggtaagcta aatgcaatat
     2161 tagaggcttc atcatgttgg cactgtgcat cttgttgtta ttcacacaag ctgacgggtt
     2221 tgatcctggc cccaatccta acaaggacat aaccaaacca gtcgttgact ttgaatcgaa
     2281 cagcgtgaaa caaaagttga agctggccaa tagtggtgag attgcagttt tggaagcact
     2341 ggccaaacga gttaaggatt ggcagtcgtt caagattgaa aacttgtctc gcccaatcat
     2401 ggggaaacca gttcatgcaa ctgagatcct cattcggtac atcgacgttt tcgatatgga
     2461 acagctacgt ccctggctcg tcaagaaagc cgagttcgac ggattcgaca agaacatcaa
     2521 acacaagatt aagaacctca atgctgcaaa gcatgaatac gccttaatgg acactgacag
     2581 tagaacctac atcaaaggca tttacaattg gattgagaaa ggcagcagag ttggggactt
     2641 accgagccaa ttgaagtcga tagctgcaag ccacaagaat ctgtttgatc ccgatgaact
     2701 cgcaacatgg agcaaggagg cagcatccaa caaagagaaa atgaaggcag aaatcagcaa
     2761 agagttagcg gcagagaaag tgcgtgagca agaagcccaa gaaaagaaaa agaatgacga
     2821 tgctgttgct aaggtgatgg aagacaagag gaaggcacag cagaagatca aagacgatga
     2881 ggacaaggca cgtgtgttac tgaaagctgc aaagctattt atggaggaca acgccaatga
     2941 gtttgccgaa cgtaggttga aggaaatcat cacaaagtat cccaacacca agtcagttga
     3001 tgaggcaaag caactacttg agaagatgaa gaaataactc aatcaagggg acacctatgg
     3061 caaagtatgt tgttaatacc aaagtcaacg gcaagcttgt ctctcaggat catcagacga
     3121 agcccgctgc tctaaagcat ctcgattcaa tctggcaaac caccaatgca atggaagcag
     3181 agattcacta ttgggcaacc tatccaaaac ttcctgcccc aaacaagaaa cccaaggtca
     3241 tcgaaagaag ggaaagaact aagcaagcgt ttccataatt gcattgcatc ggcttctttg
     3301 atcgttgtag acacttttgg ggtctcaccg gagcactatc taaatcgctt ctttggaata
     3361 cgacagagtg atggccaaca agagactcgg gactgcagcg actgaactaa aactgccgac
     3421 caagagtgtg aagggtgaaa agaaacccaa gtggcaggcg aagacgggga acaagaagtc
     3481 gagggatgcg gaggtggttg agacgccggg agtggttgta gaaacgacgg tccaggggga
     3541 gataacactc ccgccagaac ccaccgaggt gcccgtagtg gcgacaactc cggaaggtac
     3601 gggtgttgag ccagccactg tggacgaggc ggcaacgact actgctccag aaccaacctc
     3661 gcccaccgag gaaccatccg acgaaaccac acacaagaag ccccgtaagc tcaaagtcaa
     3721 agccgacggc ccacccagga agctgagcat ggtggctgca gcattacggg tgctacagga
     3781 acgcaaggtg tcgatgacct gccccgagct gatcgacgtg atggcgacag aggggttgtg
     3841 ggttagccct ggtggcaaga cgcctgcgaa tacgctgtat gctgccatca gccggatcat
     3901 caaggaacag gggaaagcat cggcgttcag gaagggggag agggggaggt ttgaggcggt
     3961 gtagcagctg gtacaacgca aacgaccccg gctgggtggc tggggtctca ccagagaaac
     4021 tatctaaatc gctgctttgg aatacgcttc agcttgaatc gctcctgtag tcctgatccg
     4081 ctaacgactt gtattttagt ttccagcaag agtcggtcga actcttgagt tacgattgac
     4141 acttggtcat caatctttgg catgtctcgg tctgcaacta caacattgta gatgtgctgt
     4201 cgggtaaatg aatctgtttc aaaaagctcg aaatatccca atatgatttc gcgaacaatc
     4261 tgcttgttcc catcggtgac ctcgttcacg tcagctcctc aaactaatac ggattgatat
     4321 gcagtcattc ctatcatagc acacatgttc tactccacca cggcccatga ggttagatgc
     4381 tagcagcggt ggcataacgc atatgaccct ggctgggtgg ctgtggccgg agttttgcat
     4441 gcaatctcgc tctgctcaaa gacctttcac gacgccttat gaatcaattc acattattac
     4501 tatcactgaa cgaaaattgc tagtatgatt caagtaacac actaatgtaa ttatctccag
     4561 ttcgagcaac ggataagggc aagacaacgg ataagggcaa gacaatggac ccgacacgtg
     4621 ccgatttcat gcaaaaagag ctgaacggca aaatggttgc gggttgggtt gtcggccgtg
     4681 tgatcggaaa cggggcttca gcagtagtgt tcgaggcgac gagagatgga ttaactgcag
     4741 cattgaaagt attggacaga gaactcgtag cgaaggtaag tgaggcacag cagctggcac
     4801 gtgttaatcg tgagcgagaa ctaatcggca aagaacaccc gaatctggta aagattcttg
     4861 acggcggcaa gtgcccggaa agtgggctgc tttttatcgt gatggaatgc ttgacctggc
     4921 aagagttacg aaaggtcatc ggcgagattc caagagcccg tatcgcgcca atcattgagc
     4981 agctcgcatc agctgcggag ttcttggaag acctcggcat tgctcacaga gatatcaagc
     5041 cagcaaacat cttaatctcg cctgacttta aggtcattaa gctaatggac ctcgggataa
     5101 ttaaaccaat cggggaggaa gggttgactg atagtgaaaa aaagccattc ataggcactc
     5161 accagtacag cccaccggag ttcatccacc gactcgaaga gaatgacaaa gaaggctggc
     5221 gagccgtaac gttttatcaa atcggcgcgg tacttcatga cttaatcatg aggaggccga
     5281 ttttccaaaa cgcagcgcac cgcactgccg accttgttaa cgcaatcgag agtgatcggc
     5341 ctgtaatcga cgcaccagac gtaccagcgg accttaggtc gctggctgca atgtgtctca
     5401 gtaaggactc cgaaaagcga cgaagagtac tgaaatggtc ttcttttcat cagccccaaa
     5461 caacgcctac tgttaatact gtgcgggagc gattgcgtga gcttcagacg agtctcaccg
     5521 gtcctagtga agctgtcacg actaaagggg ttgttgatga attggtgcta gatgggcttg
     5581 tgaactgtat caaagacagc attcggcaag aaacaatcaa gcactcggac cgcctacccc
     5641 ctgtaatctt tgcggacgta gagaggtctg ctgcaactat gactttctcg gccacatatg
     5701 gcccatcacc aactcaccta cttagtgcaa tgctctatat ccgatacaaa gttggcctga
     5761 tcgactcgat gatgagggtt ttggatattt gtgcctgcgc tacactttct tcaggtgagc
     5821 cgtcgaacga gtgcttttcg gactattcat cagtattcca gggtgcgtat atcgaagagt
     5881 tgtgtactcc cagagtgtta acagcacttt acatggcagt taccaacgct gcgaccgtgc
     5941 aagcacaagt caacaagtgt tatgcgataa actgcaattg agagggcata cctgtgtcag
     6001 atacgtggtg ggtaaaatcg acgcaactca gccctgaaca gcgcgacata gtcggcatta
     6061 ctgatcttaa gaaccgactc gtacttggcc ctcctgggtc gggaaagacc aacatactct
     6121 tgttgagggc aaagtactta atcgcagaca gtaagcctga tgtccagatc atcgtattta
     6181 ctaaggcatt acggcggttt gtggcgtcag ggcgtgaaca ctatggtgtg gccgcagaca
     6241 agatcattac cagtacgaaa tggtcccaca gacttcttcg tgacctaggt gtaaaccccg
     6301 atccacctga ggacttctcg gatgcccgga aatactactc agatatgatt tctaatctcg
     6361 ttgaacagaa gaagattgac aagctgcacg atgccattct gctggacgag gcgcaggatt
     6421 acacgtttgc agaaatccaa ttgttcgcga aagttgcaaa gcatctattt gccgttgccg
     6481 acacccgcca gcaaatatac agcgcaactg atgacccggt agaggcccta aagaaactag
     6541 ttgaacacac gcacgagctt aagtcgcatt acaggtgcgg taaaaaaatc tgcgagctag
     6601 ccgatgcatt gaggaagtac acaacgctcc acaaccccct catcaattcg atgatgtatg
     6661 acgaagagga atatccttcg tccgtgactt gcgattctgg ctcgttacgt gctcaatgcg
     6721 acttgatcgc ccagcgcttg gcagatcaga tgcgagtgta tccgggacaa tttctaggaa
     6781 tcgcttgtcc ccgaagagac ggtgttgagc gggttatgta tttgctccaa agatatcctg
     6841 agatcacgag acatgtctgc cgggtgaaag aagacgcaga atcatttgat gggcaaaaga
     6901 gcatttgtct cggcaccatc cacgacatga aaggactaga actacgtacc cttcacattc
     6961 ctgaggccga aacgttgcgc gatatgccgc atcctcgaaa tctcgccttc accgccgtta
     7021 ctcgtgcgaa gacatcattg agcttttacc atgagatgga aatgataggc tatttggaca
     7081 cagccatccg atccatactt ccgacaggcg agaagccgtc tgttgaatcg ctttttcccg
     7141 agggctagct gatgtgggcg ggcgtttaca accgtaagga catgctggat gtgctactcg
     7201 gcacgaaggg gaatcgtttg gccacccgtg cggactttgt ggacgtgctt cccaactctc
     7261 ggggccagga ccaatctgtt gttgtttggt cagccaatcc tgccgagcag tttcaaaccc
     7321 cgtctctcgt aatagttgag gacggtggcg ttcgagagtt tctagcatgg gttaccagct
     7381 atgtacagga attgtttccg tttacatcgt tttgtaggat cgctgacagg tcattgatcg
     7441 agatgtatct ggctaaacag aatggagagc attgcgcggt tgacgatggc gtcatggccg
     7501 gatt
//
LOCUS       DAORQN010000070         7216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_495_length_7216_cov_17.442815, whole genome shotgun sequence.
ACCESSION   DAORQN010000070 DAORQN010000000
VERSION     DAORQN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_7216_cov_17.442815"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..780
                     /locus_tag="PK614_05600"
     CDS             208..780
                     /locus_tag="PK614_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB81719.1"
                     /translation="MEENREHLLAQAEELFDAEQYGEALAQYRNLAKAGHPRAQLMVG
                     WIYHHGLGLQENFEEARKWYSLAAGQGSMEAQFNLGVLSMKRGDFAAGRDWYQKAASQ
                     GYLPARYRLAWSYEAGSGTTVDREKAFNLFEQTTKEGHLPSQMVIAKRLLAGYRGPTQ
                     RLVGLGLIFKTYMQTVWIAARDPQSDRIRL"
     gene            complement(818..1027)
                     /locus_tag="PK614_05605"
     CDS             complement(818..1027)
                     /locus_tag="PK614_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81720.1"
                     /translation="MNVVTWFRASVIVSGLPLASYVILVRLPLGSRMLVTGKIKTSGE
                     SSELARWTGGRNILDTFLEGERLKK"
     gene            1419..2372
                     /locus_tag="PK614_05610"
     CDS             1419..2372
                     /locus_tag="PK614_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRB81721.1"
                     /translation="MSGACFVGMDISQDTVDVAVQPGTAFQIAHTEQGLAEAVKRLQT
                     LKPALIVLEATGGLEVPLAGALAAVGLPVVVINPRQVRDFARATGQLAKTDQLDAQIL
                     ARFAEAIRPPVRPAPDAQTQALAALVARRRQLIEMLTAEKNRLRLTARPLQKRVQAHI
                     TWLENELQGLTTDLMATMRESPVWREKEEIVRSVPGVGPVLTTTLCANLQELGTLTRK
                     EVAALVGLAPFPRDSGTLKGRRAIWGGRAHVRAALYMAALVSTWKNPVIRTFYQRLCQ
                     AGKAKKLALTACMRKLLTILNAMVKSGTPWHPKMEPASAEA"
     gene            complement(2621..3802)
                     /locus_tag="PK614_05615"
     CDS             complement(2621..3802)
                     /locus_tag="PK614_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRB81722.1"
                     /translation="MDELFSDRINNVAPSFIREILKVASNPSVTSFAGGLPHRDLFPI
                     VALREATTKVFEDQGADALQYSNTEGFGPLREYIARRYWEKQGLAISMEDILITSGSQ
                     QGLDLLGKILVNEGVDVIIEEPAYLGAIQALSLYRARLNTVPISTEGMVVSKLNAVLR
                     RCKPKLMYVVPNFQNPSGITYTRDNREAVAAALRNSPTFLVEDDPYGDLRFVGSRQPS
                     FRHFLPDQTILLGSFSKTVVPAFRLGWIVPPRHLMEKLVIAKQASDLHTNTFVQRVLC
                     QYLCDNDLDTHVKTIVEFYDRQCNTMLAAIGEHFPKEILHTNPEGGMFLWVTLPNKQS
                     SMALLERAIEKNIAFVPGHPFYVGGGGTNTFRLNFSSTGKDAIHRGIALLGKLIREIP
                     A"
     gene            complement(3892..6546)
                     /locus_tag="PK614_05620"
     CDS             complement(3892..6546)
                     /locus_tag="PK614_05620"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRB81723.1"
                     /translation="MAETIRQDGSRKAHCSFSVLISHLGHMSLFSSIIKQIILPSIGL
                     VLLGIVFIAMTQFPSGDSFFAWWFQDTVDSESGEVTLRGIRDEVIIRRDALGIPVIEA
                     HNREDLAFAIGFVMAHDRLEQMVGFTLTAQGRLAEMAGPVALDIDVYLRTLGLRRVSE
                     QQYARLSESTKHFLQQFSKGVNSYLDTHRDRLPAGFRMSRYEPEPWTPLNSVDLLTLV
                     NLSLSLNLHEEISILNIVKKIGWEKAAWLVPVYPDAPLPFDEAAKLKNTHLASIALNL
                     DDLTRVSRKVDQAVLSLGIAASNNWAIAKERTQAGASILANDIHLMLSQPPMWMLMHV
                     KSPGYDVAGIALAGLPAILFGFNGHVAWGASMVMADTQDLYLEELKQIDGKIHYRYQE
                     KWFSVDEHTETFNVLGERPIKRTIRATRHGPLITEAIEAPQMNPIMSPRLDSVVDPRY
                     GLALRWGATEPDGSLDVIFEMGKSVTFQEASVHAQNIHFIHLNLVFADKDNIGWQVTG
                     RYPKRKAGKGLFPSPGWTNDYDWEGFSPLSLNPRKFNPMEGFITTANNRTVPEGYPLE
                     LSNSWFYPERAERITELIQENDKHTAEASFAMQGDQLDRFFQKLKDVLLNNREKLSHS
                     IERLPLRDREDAQRALTTLIAFDGTMSPDSCEGTIYGTFQHFITQETFLDELGPVNAP
                     AYLALVKMSVLSYSAQQDHMLQREDSPFWDDIATPVAAETKWDIFARALSRSWQFGTQ
                     QLGSDSTQWRWGKLHTYRWESQATQLKPYLGKVQQFAIGILSGFLDRGPYPAGGNHNT
                     LNQASYAIGEDFKVWIVPASRMVVDFTRDEPMWVMNSGGQSGNPASSHYADGIQLWLS
                     SENRPMPFRSQNIETQYTNMLRLTPTSH"
     gene            complement(6638..>7216)
                     /locus_tag="PK614_05625"
     CDS             complement(6638..>7216)
                     /locus_tag="PK614_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB81724.1"
                     /translation="GEGSIGEIIVKGPVVTKLYYENKLATRKAKIYESNGEVWHRTGD
                     VGYFDDQGRIWFCGRKAHICNYGDETYYSVKVEYCFNGLAGINRVALVNISLDGQKVL
                     ALCIEPTQRFMGPTARRTIVTDIVQVARCYRIPLRVIAFHPGQFPVDKRHHAKIERHL
                     LAKWAQDSLTGLRYKWNIYRISVSEQTSIASR"
BASE COUNT         1705 a         1868 c         1915 g         1728 t
ORIGIN      
        1 ctgtaagaag gttcagaaag tcggaaagta cgctcgattg gtcgttgtgt tgtgcaagat
       61 tctcagcaag aaccctgacg cacccgatgt cccgaactat cctcaaccaa agccaggtcc
      121 tacaagaaag caggacaaaa actcagatgc aggtggtcca tcagatccaa agagaaatcc
      181 acctgggcca ccgcagtaag gtcgaagatg gaagaaaatc gcgagcatct tctggctcaa
      241 gcagaagaat tattcgatgc agagcaatac ggcgaagctc tagctcagta tcgcaatcta
      301 gccaaagctg gtcacccgcg agctcaactc atggttggct ggatatatca tcatgggtta
      361 gggctacaag agaactttga agaagcgcgt aagtggtatt cgcttgctgc aggccagggt
      421 tctatggagg cgcaattcaa cttgggagtc ttgtccatga agcggggtga ttttgcagct
      481 gggcgtgact ggtaccagaa ggcggcgtca cagggatatc tgccggcgcg ctaccgactg
      541 gcttggtcgt atgaagcagg tagtgggacg actgttgaca gagagaaagc gttcaatctg
      601 tttgagcaga cgacgaagga aggacacttg ccttctcaaa tggtgattgc gaaaagattg
      661 ctggcaggtt atcgcggacc gactcagcga ttagtagggc tcggcttgat attcaagaca
      721 tacatgcaga cagtttggat agcagctcga gatccccaaa gcgatcgaat tcggctgtaa
      781 tctcgtcgtc cttcgtgccc ccttagccgc ccgccaccta tttcttgaga cgttcacctt
      841 ccagaaaggt atcgaggatg ttccgccccc ctgtccaccg agccagctca ctcgactcgc
      901 cgctggtttt tatcttgccg gtcaccagca tccgcgatcc caagggcaac ctgaccagta
      961 ttacgtatga tgccaagggc aatccactca cgatcaccga tgccctgaac caggtgacca
     1021 cgttcaccta caatcccaag ggcctcttgc tcacgaccaa aaaacaaagg gggtcaggtc
     1081 ttgcaataat acacgccatt ttcacacgca cacgatcttc aattcgacat ggtgcatccg
     1141 ccgtctccca gcggttgatt ccttcggcga aacctataga ctctgtcatg gagtaccaca
     1201 gtggaagccg gaatgtgcat gattgcaaga ccttgtatct atagaggagg tcttgcatca
     1261 tggcaccacg ggaacggggc agatcgcaaa gatccgggtc tggaagcaag aagaccgtgg
     1321 gtcagctcgg gtcgtcccgt tcctccggtc agcaacccga acagttgagg gggccacgag
     1381 cccgcatcgt gcaaggacgg gagacacgga ggtgcaccat gagcggagca tgttttgtcg
     1441 gcatggatat ctcgcaagac accgtcgatg tggcggtcca gcccgggacc gcattccaaa
     1501 tagcgcacac tgagcaggga ctcgcagaag cggtcaagcg actgcagacg ctcaagcctg
     1561 ccttgatcgt gcttgaagcg acgggcgggc tggaagtgcc gctggccggg gcactggccg
     1621 ctgtcggctt gcccgtcgtg gtgatcaatc ctcggcaagt gcgggacttt gcgcgggcca
     1681 ccggccaatt ggcgaagacc gatcagctgg acgcccagat cctcgctcgc tttgcggaag
     1741 caattcgtcc tcctgtccgt ccggcccctg atgcgcagac gcaagcactg gcggcgctcg
     1801 tggcgcggcg tcggcagctg atcgagatgc tgacggccga aaagaatcgg ctccgtctca
     1861 ccgcgcgccc tcttcagaaa cgggtgcagg cgcatatcac atggctcgag aatgaactgc
     1921 agggcctcac gaccgatctg atggcgacga tgcgcgagag tccggtgtgg cgcgagaagg
     1981 aagagatcgt ccggagtgtg ccgggcgtgg gcccggtgct gaccacgacg ctctgcgcga
     2041 acctccagga gctggggacc ttgacgcgca aagaagtggc cgccttagtc gggctcgccc
     2101 cgtttccccg cgacagcggg accctcaagg gccggcgagc gatctggggc gggcgggctc
     2161 atgtgcgggc ggcgctgtat atggccgctc tggtatctac gtggaaaaac ccggtcattc
     2221 gcacctttta tcagcgactc tgtcaggcgg gcaaagcgaa gaagttggca ttgacggcct
     2281 gcatgcgcaa actgctcacc atcttgaatg cgatggtgaa aagcgggacg ccgtggcatc
     2341 ccaagatgga accggccagt gccgaggcgt aggagagcct ggactccatg gcgatcgtcg
     2401 ttcctacgcc aattgtgtgc gacacatttt catggtgcat gggaaaatac ccagttcgac
     2461 tcttgacagc acagacagtt gctgaccctt ctgtgtcgag ataatcgcct agtctttcag
     2521 gtctggagat ctcgtcagtc ctgttgagac cgccggttac ttactcagga ttttatacgg
     2581 ctttaagtgc catagcgaac tagtgagctc ggttggccgt ttatgctggg atctctctta
     2641 ttaactttcc cagtaatgca attcctctat gtatcgcatc tttgccggtc gaggagaaat
     2701 tcaaccgaaa ggtgttggtc cctccgccgc cgacataaaa tggatgtccg ggaacaaagg
     2761 caatattttt ctctatagcc cgttcaagca gagccatcga cgactgcttg ttcggaagcg
     2821 tgacccacag gaacatcccc ccttccgggt tcgtgtgcaa aatctctttg ggaaaatgtt
     2881 ctccaatagc ggccaacatg gtgttgcatt ggcggtcata gaattcgacg atggtcttca
     2941 cgtgtgtgtc taggtcatta tcacaaagat attgacagag cacgcgttgc acaaatgtgt
     3001 tcgtgtgaag gtccgaagcc tgttttgcaa tcaccaattt ttccatgagg tggcgaggcg
     3061 gcacgatcca ccccagccgg aaagcgggaa cgaccgtctt ggaaaacgaa ccgagcagga
     3121 tggtttggtc tggcaagaag tgcctaaaag aaggctgtcg cgagccgacg aaacgaagat
     3181 cgccgtaagg gtcatcttcc acgaggaagg ttggcgaatt tcgcaaggcg gcggcgactg
     3241 cttcacggtt gtctctggta tatgtgattc ccgaagggtt ctggaaattc ggtaccacat
     3301 acattagctt tggcttgcaa cgcctcagaa cggcattaag cttcgaaaca accatgcctt
     3361 cagttgagat gggcactgtg ttaagccgtg ctcggtatag cgaaagagct tgaatggcac
     3421 ctaaataggc aggttcttcg atgatcacgt cgacgccttc attgacaaga attttgccga
     3481 gtaggtccaa gccttgctga gagccgctcg tgatgagaat atcctccatc gaaatagcca
     3541 aaccttgctt ctcccaatag cgtcgagcga tatactcccg aagtggccca aatccctcag
     3601 tgttactgta ttgcaaagcg tcggcacctt ggtcttcgaa aaccttggtc gtggcttctc
     3661 ggagagcaac gatggggaac aggtcgcggt ggggtagccc tccggcgaag gaggtcacgg
     3721 atggatttga ggccaccttc agtatttccc ttataaagga aggggccaca ttgttaatgc
     3781 gatcggagaa cagttcatcc attggcaggc tcaattactg gatactgaaa cgtgctgcag
     3841 tttctcctca actatctcaa tcgtactggg aggagagcag tctacatctc tctagtgaga
     3901 ggtgggggtc aatcgaagca tgttggtgta ctgtgtttca atgttctgcg agcgaaacgg
     3961 catcggcctg ttctccgatg aaagccagag ttggatgcca tctgcatagt gtgaacttgc
     4021 cggattccca ctttgacccc cactgttcat aacccacatt ggctcatccc tagtaaaatc
     4081 gaccaccatc ctggatgctg gaacgatcca gaccttgaaa tcctcgccaa tggcatagga
     4141 ggcttgattc agggtattgt ggttccctcc agctggataa ggaccacgat ctaaaaagcc
     4201 ggacaatatg ccgatcgcga actgctgtac cttgcccagg tatggtttta actgcgtcgc
     4261 ctggctctcc caccgatacg tatgtaactt gccccatcgc cattgagttg aatctgagcc
     4321 aagttgctgt gtcccgaact gccaggatcg actcagggca cgggcgaaaa tgtcccattt
     4381 ggtttcagct gccacaggtg tcgcgatgtc atcccaaaac gggctatcct cccgttgaag
     4441 catgtgatcc tgctgagcgg agtagctaag gacactcatt ttcaccagag ccaaatacgc
     4501 aggggcattg acaggcccga gttcgtcgag aaaggtctct tgagtgatga aatgttggaa
     4561 agttccgtag atcgtgcctt cacaggagtc aggtgacatg gttccatcaa aggcaattag
     4621 cgttgtcaac gctcgttgcg cgtcttctct atctctaaga ggtaatctct caatcgaatg
     4681 ggacaacttt tcgcgattat tcagtagtac gtccttgagc ttttgaaaga agcgatcaag
     4741 ttgatccccc tgcatggcaa aagaggcttc agctgtatgt ttatcattct cctggatcag
     4801 ttcggtaatg cgttcagcgc gttctgggta gaaccatgag ttgctcagct ctaacgggta
     4861 cccttctggg acagtgcggt tattcgcggt ggtgatgaat ccttccattg gattgaattt
     4921 tcgcgggttg agactgagcg gcgaaaatcc ttcccaatcg tagtcgttgg tccaaccggg
     4981 cgaaggaaac agacccttac ctgccttgcg tttcggatag cgcccggtca cttgccatcc
     5041 aatattgtct ttatcagcaa aaactaggtt tagatggatg aagtggatgt tctgtgcgtg
     5101 cactgaagct tcctgaaatg ttaccgattt ccccatttca aaaattacat ccaagctgcc
     5161 atctggctct gttgcacccc agcgaagcgc tagcccgtat ctaggatcaa ccacgctatc
     5221 gaggcgaggc gacatgatag gattcatctg tggggcttcg attgcttcgg tgattagtgg
     5281 cccatgacgg gtagcgcgaa tggtgcgttt tattggtctc tctcctagca cgttaaatgt
     5341 ctccgtatgt tcatctaccg aaaaccattt ttcctggtag cgataatgta ttttgccgtc
     5401 aatctgcttg agttcctcta ggtataagtc ttgagtatct gccatcacca tggaagctcc
     5461 ccaagcgaca tggccattaa atccaaagag aatagctggt aggcctgcca aagcaatacc
     5521 agccacgtcg taaccggggg atttcacatg catcagcatc cacatcggag gttggctaag
     5581 catcaagtga atgtcattgg caagaatgga tgcgccggcc tgagtccgct ccttcgcaat
     5641 tgcccagtta ttactggcag ctatcccaag agatagcact gcctggtcaa ccttccggga
     5701 cactcgggtc aagtcatcaa ggttcagggc aatcgatgcg agatgggtat ttttcagctt
     5761 cgctgcttcg tcgaatggca atggtgcgtc cgggtaaact ggaaccagcc aagctgcctt
     5821 ttcccatcca attttcttca cgatgttaag gatacttatt tcctcgtgaa gattgagact
     5881 caaggaaaga ttcactaggg tcaggagatc gacggaatta agtggcgtcc atggttccgg
     5941 ctcatatcta gacattcgga aacccgccgg caagcgatcg cggtgcgtat cgagatagga
     6001 attgacgcct ttggagaatt gctgcagaaa gtgttttgta gactcactca gtcgcgcata
     6061 ctgttgttcc gatactctac gtaagcccag cgtccttaga tagacatcga tgtctaatgc
     6121 aacaggcccc gccatctccg ccaatcgtcc ttgggcggtc agtgtgaagc ccaccatttg
     6181 ctccaaccga tcatgtgcca taacaaagcc tatggcgaag gccaaatctt ctcggttgtg
     6241 agcttcaatc actggaatgc cgagtgcatc tcgacgaata ataacttcgt cacgtatccc
     6301 gcgaagggtg acttccccgc tttctgagtc caccgtgtct tgaaaccacc acgcaaagaa
     6361 gctgtctcca ctggggaatt gggtcatggc gataaagact atgcctagca ggactaggcc
     6421 gatacttgga agaatgatct gcttaataat tgaactgaat agactcatgt gaccgagatg
     6481 acttatcaat acagagaaac tgcaatgcgc ttttctggaa ccgtcttgcc ggatggtttc
     6541 ggccattgat cagaatcgtg ttaagcttcg agtgggccaa tgatttccta tgtcaggcgg
     6601 tggtcctgca ttcggtatga tgctggagta tgccgctcta cctagatgcg atactcgtct
     6661 gctcactcac gcttatacgg taaatattcc atttgtatct cagtccagtt aaagagtcct
     6721 gtgcccattt agccaataga tgccgttcga ttttcgcatg atgccgttta tctacaggga
     6781 attgcccggg atggaaggca atgactctta gcgggatacg atagcaccta gccacttgca
     6841 caatgtctgt gacaattgta cgtctagctg tcgggcccat aaatctctgt gtgggctcaa
     6901 tgcacagggc aagaactttc tgcccatcca gggagatatt tactaatgct acgcgattga
     6961 tacccgcaag accattgaag caatactcaa ccttaaccga atagtaggtc tcgtctccat
     7021 aattgcaaat gtgtgctttg cgtccgcaaa accaaatgcg tccttgatcg tcaaaatatc
     7081 cgacatcccc tgtccggtgc cacacctcac catttgattc gtaaatcttt gctttcctag
     7141 tcgcgagctt gttctcatag taaagctttg ttacaaccgg tcccttcacg atgatttcgc
     7201 cgatgcttcc ctcgcc
//
LOCUS       DAORQN010000071         7097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_505_length_7097_cov_10.429423, whole genome shotgun sequence.
ACCESSION   DAORQN010000071 DAORQN010000000
VERSION     DAORQN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_505_length_7097_cov_10.429423"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PK614_05630"
     CDS             <1..1487
                     /locus_tag="PK614_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81725.1"
                     /translation="ATDTTWIQDLLTWVLSGPETDQRETKRVSGKMVGKALETVCALA
                     NTQGGWLVLGVEDADKAQGQQRLYGIAENPEAVDELLRKLNTHLLPTVDEIRAFRVPC
                     TLRDGSEGVLVALLVPASAKVHSILDDGTWMRGQRSNREMTATEITELSYRRGVRSAE
                     SEPVDVDFELLDTDIWRLYLRGRNLAPTGIPDQLYRAGLARKVGGELRPLRAAVLLFA
                     DQPGALLAAAGTRADVRVFHYQGNTITSGAVPNLKKPPKTLSGPLYHLIAQAYAYVLD
                     ELASGLTLAASGFRTLHRYPERVIKEAITNAIIHRDYRMNRDIQIRIFDNRIEVVSPG
                     LFPGRITAATVHRTGSFARNPLLAGNLREFPEPPNVDAGEGVKMMFSLMRAGNLYPPQ
                     YRELRDQAQEAIAVMLFNEARPPIWEQVSDWIDREGPLANSDLCRIAGLDTLKSSKLL
                     KRWVEQGLLVPDTSRGKRHVVYQKPVSDTESPFKTSLLSEPPDN"
     gene            1642..3102
                     /locus_tag="PK614_05635"
     CDS             1642..3102
                     /locus_tag="PK614_05635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRB81726.1"
                     /translation="MNTSTLVQKVWNFCHTLRDDGVGYGDYVEQLTYLLFLKLAHEYA
                     QEPYHRDTHIPKGYDWATLRTKTGEPLEAQYLGTLHKLGQEPGMLGAIFFKAQNKIQD
                     PAKLSRLVQMIDAENWIGLDADTKGDLYEGLLQKNAEDTKSGAGQYFTPRALIDAMVA
                     CVRLEPLKTIADPACGTGGFFLGAYNWLNRPGAKLDKRQNEFLRNKTFHGNEIVPSTR
                     RLCLMNLFLHNIGELDGEPSVERSDALITEPKQKVHYVLANPPFGKKSSMTITNEEGE
                     EDKEALTYERQDFWETTSNKQLNFLQHIVSMLRVDGKAAVVLPDNVLFEGGAGEKIRR
                     KLLETCDVHTILRLPTGMFYAQGVKANVVFFDAKPKDGRIHTTGVWFYDLRTNKHFTL
                     KTRPLKREDLQEFIASYHPENRHERRETERFKFFSYQDLMARDKASLDIFWLKDDSLD
                     NLDDLPPPDVLQQEIIEHLEAALASFRDVAAGLQRK"
     gene            3276..3839
                     /locus_tag="PK614_05640"
     CDS             3276..3839
                     /locus_tag="PK614_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HRB81727.1"
                     /translation="MGDDLIKHLGPLAALAGVWEGDKGADVAPDDDRGTEHNAYRERL
                     TFEPIGPVNNHEQQLYGLRYSLVVFRLSDGTAFHEESGYWLWDAAAKQVLRCFSVPRG
                     VTVLAGGTVEPGATSFQLAADVGSDTYGICSNPFLDREFKTVRFEVTVTVHGPDSFSY
                     HEDTQLKMKGRNDLFHHTDTNRVTRVK"
     assembly_gap    3974..4012
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4013..4515
                     /locus_tag="PK614_05645"
     CDS             <4013..4515
                     /locus_tag="PK614_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81728.1"
                     /translation="LATGCAGLPQTSRTAIIHEVKFEGSLAPADLTVHVGDEIRWVNH
                     RTLPVLIDIPGLNAEMLSCDRGFSGMMGGVSELKELDPSKSASLCFSKPIVISYNARM
                     KSADPGGHEDRARHHPRRRPRPVVCAYPAYAALPSPSPRGLSHGSGVRVRSSDQRFRL
                     CRMSSR"
     gene            complement(4584..6380)
                     /locus_tag="PK614_05650"
     CDS             complement(4584..6380)
                     /locus_tag="PK614_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRB81729.1"
                     /translation="MVWSMYPYGLIGNCHVSAHIHESGSVDWLCLPRPDSDPVFGRIL
                     DPEGGHFSISSPTSSAQVKTTQRYLPNTNILVTEVSTSNGDTFKITDFCPRFEQYGRV
                     YRPAALFRIVEPLAGTPSIRVSCRPVTGWGKEYARGMRGNSHVRYDIRGEYLRLLTNM
                     PLTYLYEERPFALTEKTYFALTWGLGIEDDLAKVSHEFLDQTTRYWRMWVKHCSIPVL
                     HQEEVIRSALALKLHCYEDTGAILAALTTSLPEEPGGPRNWDYRFCWLRDAYFSLTAF
                     HNLGHFEEMEGFLKFLLNIAYTHEHSRERLAPVYTLSQDLPLPETEHRNWAGFCGSAP
                     VRNNNQAAEHIQNDVYGELVLALTPIFSDNRFYDLRTKDQEQLVANLARLCDRTIAQP
                     DAGLWEIRNGWQEHSFSNLMCWAGLDRAHRMHKAGFLPSLTLDLDAARARAAQALLNA
                     TKDGSLRNGPKDDTYDASLAQAAILGFPNRDVCETTMQSIARALAVQAGGKETGFYFR
                     YLRTDDFGRPQSSFVICSFWVVQGLARLGRMAEAQQIMAQVLTGANHLGLFSEHFVPE
                     TRTQLGNFPQAYSHVGLINAAFAVSPSWTTVL"
     gene            6419..>7097
                     /locus_tag="PK614_05655"
     CDS             6419..>7097
                     /locus_tag="PK614_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRB81730.1"
                     /translation="MRLSLRFILPLLLVLGVVAYSVVPLVDSLTLKWFTRDLESRSKL
                     LASTMEVPLADLVVSRSRTKIFAYFHKVIQDERLYALGFCDTQQRLLYQTLTYPDQLS
                     CESLAHLAPGSSEVVEFAQGPLHVVVATIQSGGKAQGRLMLVHDMSFVHQRSTDTKWY
                     IFYLFVGLAGLISAVTVLVAQLSWRGWVAGVRAMLTTKGLPPESAGAHSSELHPVAQD
                     LHALVREL"
BASE COUNT         1489 a         1895 c         2187 g         1487 t
ORIGIN      
        1 ttgcgaccga tacgacgtgg atccaagact tgttgacctg ggtgttgtca ggcccggaaa
       61 ctgatcagcg tgaaaccaag cgcgtgtcgg ggaaaatggt cggcaaggcg cttgagaccg
      121 tgtgcgcatt ggccaacaca caaggtgggt ggttggtgct gggcgtggaa gatgccgaca
      181 aagcacaagg ccaacaacgg ctgtatggca ttgccgagaa tccggaagcc gtggatgagc
      241 tgctgcgcaa gctgaacacg catctgttgc cgacggttga tgagattcga gcgttccgcg
      301 tgccgtgcac acttcgtgat gggagcgaag gtgtactggt cgcgctgctc gtacctgcca
      361 gcgccaaggt gcattccatt ctggatgacg ggacctggat gcgcggccag agaagcaacc
      421 gtgaaatgac ggccacggaa atcacggagc tgtcgtaccg ccggggtgtg cggagcgcgg
      481 aatcagaacc ggtggatgtg gatttcgagt tgctggatac ggacatctgg cggctgtatc
      541 tgcgcgggcg taatcttgcg cccaccggga ttcccgatca actataccgt gctgggttgg
      601 cgaggaaggt cggcggcgag ttgcgtccgc ttcgggctgc cgtgttgttg tttgccgacc
      661 agccgggcgc cttactggcg gctgcgggca cccgggccga tgtgcgagtg tttcattacc
      721 agggcaatac catcacatcg ggtgccgtgc ccaaccttaa gaagccaccg aagacgctct
      781 cagggccact ctaccacctg attgcgcagg catacgccta cgtgctggat gagctcgcga
      841 gcggcctgac gcttgctgct tcaggctttc gcacgctgca ccgatacccg gaacgtgtca
      901 tcaaggaagc cattaccaac gcgatcatcc accgtgacta tcggatgaac cgggatattc
      961 aaattcgtat cttcgacaac cggattgaag tcgtcagtcc ggggttgttt cccggtcgta
     1021 ttaccgccgc caccgtgcat cgcacgggct cattcgcacg taatcccttg ctcgcgggca
     1081 atctgcggga gtttcccgaa ccgccgaatg tggatgccgg tgaaggggtg aagatgatgt
     1141 tcagcctcat gcgggcgggc aatctttatc ccccgcagta tcgagagctc agggatcagg
     1201 cgcaggaagc cattgctgtg atgctgttta atgaagcacg tcctcccatc tgggaacagg
     1261 tgagcgactg gattgatcgc gaagggcctt tggctaacag cgatctgtgc cggattgccg
     1321 gattggatac gttgaagtcc tcgaagcttc tgaagcgctg ggtggaacaa ggtttattgg
     1381 tccccgatac tagccggggc aaacgccatg ttgtgtatca aaagccggtg tccgatacgg
     1441 aatctccctt taagacatcg cttttgtccg agcctccgga taattgagag gggcaggtcg
     1501 taactaaaag actgggactc gctctttttt cattatccag gcggctagat aatgaaacga
     1561 aaaaagtggt aaccatttga ttattcaaaa gataactgcg cgttcattat ccctattgga
     1621 ttcctgccca aaaacagccc catgaacacg tctaccctgg ttcaaaaagt ctggaacttc
     1681 tgccataccc tgcgcgacga cggcgtgggg tacggcgact atgtggagca gctcacgtat
     1741 ctgctgttcc tgaagctggc gcacgaatat gcccaggagc catatcaccg cgatacccat
     1801 attcccaagg gctatgattg ggccacgctt agaaccaaaa cgggcgagcc gttggaagcg
     1861 cagtatcttg gtacgctgca taagctcggt caggaaccgg ggatgctcgg cgccattttc
     1921 ttcaaagcac agaacaagat ccaggatccg gccaagctgt cgcgcctggt gcagatgatc
     1981 gacgccgaga attggatcgg tctcgacgcg gacactaaag gcgatttgta tgaggggctg
     2041 ctccaaaaga acgccgaaga caccaagagc ggcgccggcc agtacttcac cccgcgcgcc
     2101 ttgatcgacg ccatggtggc ttgcgtacgc ctcgaaccac tgaaaaccat tgccgatcct
     2161 gcctgtggca ccggcggatt ttttcttggc gcctacaact ggttgaaccg gccgggtgcc
     2221 aagctggata agcgccagaa tgagtttttg cggaacaaga cgttccatgg caacgagatc
     2281 gtccccagca cccgccggtt atgcttaatg aacctgttcc tgcacaacat cggtgaactc
     2341 gacggcgagc cttccgttga acgctcagat gcgttgatca cagagccgaa gcaaaaggtg
     2401 cactacgtgc tggccaaccc gcccttcggc aagaaaagca gcatgaccat caccaacgag
     2461 gagggtgaag aagataaaga ggcgctgacc tacgagcggc aggatttttg ggaaaccacg
     2521 tcgaacaagc agctcaattt cttgcagcac atcgtgagca tgctgcgtgt ggacggcaaa
     2581 gcggctgtgg tgttgccgga caacgtgcta ttcgaaggtg gcgccggcga aaagatccgc
     2641 cgcaagctgt tggaaacctg cgatgtgcac acgatcttac gtctgcccac cggcatgttt
     2701 tatgcgcaag gggtcaaggc gaacgtggta tttttcgacg ctaaaccgaa ggatggacgg
     2761 atccacacca cgggagtgtg gttctatgac ctacgcacca ataagcactt cacgctgaag
     2821 accagaccct tgaagcgtga ggatctacag gagttcatcg cctcctatca cccagagaac
     2881 aggcatgagc gcagagaaac agagcgcttc aagttcttca gctaccagga tttgatggcg
     2941 cgcgacaaag cgagcctcga tatcttttgg ctcaaggatg acagtctgga caatctcgat
     3001 gacctcccgc ccccagatgt gttgcagcag gagatcattg agcatctgga ggcagccttg
     3061 gcgtcgttca gggatgtggc cgccggactg caaaggaagt agtctgaggt tcacgttccg
     3121 ccgccttcgc ggggcaatcg ttctgctcga cacactccgg tttgtttcaa tttgagtctc
     3181 tcgcccggct tctgctagaa gagcacccac gcggtgtccc gatttctcta ccggaggtat
     3241 tttggaacag gcaaccgcat gaaaggaacg acgacatggg tgacgatctg atcaagcatc
     3301 tcggtccgtt ggcggcgttg gccggtgtgt gggaaggcga taagggcgcc gatgtggcac
     3361 ctgacgatga ccgaggcacg gaacacaatg cctatcgcga gcggttgacc ttcgagccga
     3421 ttgggcctgt taacaatcat gagcaacagc tatatggctt gcgctattcg ctcgtggtct
     3481 ttcgactgag cgatgggact gcgtttcatg aggagagcgg atactggcta tgggatgcgg
     3541 cggcgaagca ggtgttgcgc tgtttcagcg tgcctcgcgg cgtgacggtc ctcgcgggtg
     3601 gaacggtgga gcctggcgcc acgtcgtttc aactcgcggc ggacgtcgga tcggatacct
     3661 acggcatctg ttcgaatccg ttcctggatc gggagttcaa gaccgtccgt tttgaagtca
     3721 cggtgaccgt gcacggcccc gacagtttca gctaccacga agacacacag ctgaagatga
     3781 agggacggaa cgatctgttc caccacaccg ataccaatcg ggtaacacga gtcaaataac
     3841 gcagttaggc ttccacgcga aaatactgag ccccgagctt gtaagtgagt tcggggaacg
     3901 tggtagagta aaaagggtgg attcgaaaga gcaagaatag accgctcaga ttctgagagt
     3961 ctaaaaatac tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttggca
     4021 actggttgtg cggggctgcc gcagacctcg agaacggcga tcatccatga ggtcaaattt
     4081 gagggcagtc tggcgccggc ggatctgacc gtacacgtcg gtgacgaaat acgctgggtg
     4141 aaccatcgga cgctccccgt gctcatcgat attcccggcc tgaatgcaga gatgttgtct
     4201 tgcgatcgcg gcttctccgg catgatggga ggggtgagcg agttgaagga attagacccg
     4261 agcaaaagtg caagcttgtg tttcagcaag cctattgtca tcagctacaa tgcccgcatg
     4321 aaatcagccg atccgggggg gcatgaagat cgagcccggc accatccgcg tcgtcggccc
     4381 cgcccagtag tctgtgctta cccggcttac gccgcgttac cctctccttc gccccggggc
     4441 ttgtcgcacg gctccggggt tcgagttaga tcctcagacc agagatttcg attgtgccgg
     4501 atgtcgtctc ggtaggcgcg cactgtcggc gcggcactgt tgtacggggc agaggcggcc
     4561 gtctgactgc acaggcgtga gagttagagc accgtcgtcc aggagggact gacggcgaag
     4621 gcggcgttga tcagaccgac gtgggaatag gcttgtggaa agtttccaag ctgagtgcgg
     4681 gtttcgggga cgaaatgttc ggagaagagc cccagatggt ttgcgccggt gaggacctgg
     4741 gccataatct gctgcgcttc agccattcgc cccagtcgcg cgagtccttg gacgacccag
     4801 aaggaacaga tgacgaagct ggattgcggc cgtccgaagt cgtcggtgcg caggtagcgg
     4861 aaatagaagc cggtttcctt cccgccggct tgcacggcca gcgcgcgcgc gatgctctgc
     4921 atcgtggttt cgcacacgtc gcggttggga aagccgagga tcgccgcttg tgccagcgag
     4981 gcatcgtagg tgtcgtcctt gggaccgttg cgcagtgagc cgtctttcgt ggcgttcaac
     5041 agcgcctgtg ccgcccgtgc gcgcgccgcg tcgaggtcga gcgtcaacga ggggagaaat
     5101 cccgccttgt gcatgcggtg agcccggtcg agtccggccc aacacatcag attcgaaaag
     5161 gaatgttcct gccagccgtt gcgaatctcc cacaagcccg catccggttg ggcgatggtt
     5221 cgatcgcaga gtctggccag gttggcgacg agttgttcct ggtctttggt ccgcagatcg
     5281 tagaaacgat tgtcggagaa gatcggggtc agtgcgagga ccagttcgcc gtagacatcg
     5341 ttttgaatat gttcggcggc ctgattgttg ttacgtaccg gcgcgctgcc gcagaagccg
     5401 gcccagttcc ggtgttcggt ttccggcaag gggagatcct ggctcaaggt gtagaccggc
     5461 gcaagccgtt ctcgtgaatg ctcatgcgtg taggcgatgt tgagcaggaa tttgagaaac
     5521 ccttccatct cttcgaagtg gccgagattg tggaaggcgg tgagggaaaa ataggcgtcg
     5581 cgcaaccagc agaatcgata gtcccaattc cgcggaccgc ccggctcctc cgggaggctg
     5641 gtggtcagtg cggcgagaat ggcaccggtg tcctcgtagc aatggagttt gagcgcgagc
     5701 gcggaccgaa tgacctcttc ctgatgcagc acgggaattg aacaatgttt gacccacatg
     5761 cgccagtagc gagtcgtctg atcgagaaac tcgtggctga ccttggcaag atcgtcctcg
     5821 atgcccaggc cccaggtcag ggcgaaatag gttttctcgg tgagcgcgaa cggacgttct
     5881 tcgtacaaat aggtcagggg catgttggtg agcagccgga ggtattcccc gcggatgtca
     5941 taccggacgt ggctgtttcc gcgcatgccg cgggcatatt ctttccccca tccagtgacc
     6001 ggtcggcaac tgacgcggat ggacggcgtg cccgccagcg gttccacaat gcgaaagagg
     6061 gcggcaggcc gatagacgcg gccatactgt tcgaagcgcg ggcagaagtc cgtgatctta
     6121 aaggtgtcgc cgtttgacgt ggacacctcc gtcaccagga tatttgtatt agggaggtag
     6181 cgctgcgttg tcttgacttg agcggagctg gtagggcttg atatggaaaa gtgccctcct
     6241 tccggatcaa ggattcgacc aaagaccgga tcactgtccg gtctgggcag gcagagccag
     6301 tctacagacc cgctctcatg aatatgggcg gagacgtgac agttgccgat gagtccgtag
     6361 gggtacatac tccaaaccat aagagatttt cttctcaaag taaactgtag gagcaaacat
     6421 gcggctgtcc ctccggttta tcctccctct cttgctggtt ttgggcgtcg tggcctatag
     6481 cgtggtgccg ctggtggact cgctcacgct caagtggttc acgcgcgatc ttgaaagccg
     6541 gtccaagttg ctggccagca cgatggaagt gccgttggcg gacctggtcg tttcgcgttc
     6601 acgcacgaag atcttcgcct actttcacaa agtcattcag gacgagcggc tctatgcgct
     6661 gggtttctgc gatacccagc agcgcctgct ctaccagacg ttgacctatc ccgaccagct
     6721 gtcctgcgaa agtctggcgc atctggcgcc gggctcctcg gaagtggtgg agtttgcgca
     6781 gggaccgttg cacgtggtgg tcgcgaccat tcagtccggg ggcaaagcgc agggccggct
     6841 gatgctggtc cacgacatga gttttgtgca ccagcgcagc accgatacga agtggtacat
     6901 cttttatctc ttcgtcggac tcgccgggtt gatttccgcg gtgaccgtgc tcgtcgcgca
     6961 actcagctgg cgaggctggg tggccggagt gcgggccatg ttgaccacta aagggttgcc
     7021 gcctgaatcc gccggagccc attcgtccga actgcatccg gtggcgcagg atctccatgc
     7081 gctggtgcgg gaattgg
//
LOCUS       DAORQN010000072         6725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_524_length_6725_cov_52.267166, whole genome shotgun sequence.
ACCESSION   DAORQN010000072 DAORQN010000000
VERSION     DAORQN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_6725_cov_52.267166"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PK614_05660"
     CDS             <1..1552
                     /locus_tag="PK614_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB81731.1"
                     /translation="QRATLNFETLLEVTNALNSQRDIDSLWRVIADQIRKVIPWDRAG
                     ITLYESQTDSFRFYAVITNMAAPALAHDSVIPREGSAVGWVHDNRRLHIREDLQKEQV
                     FLEDRYYVQEGLGRMINLPLLVREHCLGTLNIGSVQTGHLDQDDCKFLQQVATQIAYA
                     IDHVLAYEQIQQLSDRLRRENEYLAEEVKASRNLRLVVGTSLSFRKVVDLLKAVAPTD
                     TTVLLLGETGTGKEVLAQALHDLSLRSHKPFIRVNCAALPSGLIESELFGHERGAFTG
                     AQLRRAGRFELAHTGTLFLDEIGEMPLETQAKLLRVLQDGMVDRIGGTQPIAVDVRLV
                     AATNADLQAAVRQGTFRADLFYRLHIFPIALPPLRDRREDIHLLAQHFLAQIGGKLKR
                     AHLAFDPQSMARLLTYNWPGNVRELQNVIERAVILSGSSQVTVDEMLLPVVQAVHSPS
                     SSQPVSLGDLERHHIIEVLERTKWRIYGEQGAAHLLGLNPETLRSRLRKLGLRRPAGI
                     SPEPTALP"
     gene            1923..2204
                     /locus_tag="PK614_05665"
     CDS             1923..2204
                     /locus_tag="PK614_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81732.1"
                     /translation="MKITSVWRGNKPNHKKKRTVPKLRIRWTQLGLVDPSVDSPETPM
                     DEIRRAVTNLASSGFGAETRARSEQTQARPARAHARTSATRTTRRPSGS"
     gene            complement(2486..2833)
                     /locus_tag="PK614_05670"
     CDS             complement(2486..2833)
                     /locus_tag="PK614_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81733.1"
                     /translation="MKVSDQNQDAPRAASQWVRIPDGTMVQHRLDGQKGHIDGLTEIV
                     NGPSRNPDGRTQYRINVGKGDRVLIAEEDLLILTDAEGLVRMAKEKGEYRSLVSKQLR
                     GVFGEDRFVIKAS"
     gene            complement(2863..4158)
                     /locus_tag="PK614_05675"
     CDS             complement(2863..4158)
                     /locus_tag="PK614_05675"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB81734.1"
                     /translation="MQTTAFTSFNSLGVSPTLLRNLTKAGFAEPTAIQAQAIPHALAG
                     RDVLGCAQTGTGKTAAFVIPMLERLSGIPKGQPRALILAPTRELAIQIQATIDTLGRD
                     LQLFATTVVGGADMQAQVRGLRQRPDIIVATPGRLLDHMWNGTISLLAMSILVLDEAD
                     RMLDMGFAQQINQILDAMPEERQTLLFSATMPNDLARLAQASVKDPVRVMVTKSATTA
                     DGVTQAVHHTTHDRKNGLLMSLLQSESDTVLVFARTKHRADRLGNLLDSAGHRVAVLH
                     GGRTLPQRRAALEGFRRGTYRVLVATDIAARGIDVANIAHVINYDVPNCPEDYVHRIG
                     RTARMRTTGRATTFVTSEDQEQLRAIERLLGQAVPRAEGSPASMSASPRPDGHAPRSE
                     PERRRRRGGSSQVWSQSADGGPREANGGIKNGSDLGPKS"
     gene            4465..4752
                     /locus_tag="PK614_05680"
     CDS             4465..4752
                     /locus_tag="PK614_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81735.1"
                     /translation="MRMIAILLFLFLWSDGPVLTANAASESSGTGKAGSNQGLTLDEI
                     GRGLKSAAKNIEEEIPKIGPAIGKTFKQVTGGEKEKSPPKPPAQNTSKDKK"
     gene            4777..5196
                     /locus_tag="PK614_05685"
     CDS             4777..5196
                     /locus_tag="PK614_05685"
                     /inference="COORDINATES: protein motif:HMM:NF023520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HRB81736.1"
                     /translation="MRRLISIVLLLSALIQTGGCLSARAPACLPGEHPAVLESLYFGT
                     TKPGGNVSPEEWAAFVKDIVTPAFPEGLTSWQASGQWQKSTGAIEYETSRILQLTHEQ
                     NMAKEAAIRRIMIIYQQTFQQDAVMRVRSPACLSFKG"
     gene            complement(5217..6305)
                     /locus_tag="PK614_05690"
     CDS             complement(5217..6305)
                     /locus_tag="PK614_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81737.1"
                     /translation="MRIVDIRSQVLGYSVMLLVSMSTMAWAEEPPLPLSGEGQARGVV
                     IGIVGIPVRIVSPATTSSGVLSVGDVISAGEELRVGTGEQVTLLWDRRAVLTLNEGAR
                     LNVQELHRGQTELLLHQGKVRVALSYNAGRMTDTLTLRTPLARAVSRGGIVEATVVGE
                     ERQSLLARLVTAPPGDTLRVLEGQARVEPLTGDRKAFPLKAGREVSLRTGMVGSLSEF
                     QESSHILQPLAVREEHRVLPSPLTRQIVGTQVGHALELDQEILQASSAGGEKELRGPS
                     SKGTILATSIGLPVVPLSQASSPASVTGTSTGVPAALPPPAVPPVQSAGVGSLGPAQS
                     GGLNSRNLLREILTEVGRGGRGHGKKDR"
     gene            6482..>6725
                     /locus_tag="PK614_05695"
     CDS             6482..>6725
                     /locus_tag="PK614_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81738.1"
                     /translation="MQEKLRSPERGTNVMICTTQQRGRTSIDTHPQFSLVLMMILILM
                     AMAVPPGSAQAQAADAEVLVAEGILAYEAKRYDEAAV"
BASE COUNT         1475 a         2101 c         1835 g         1314 t
ORIGIN      
        1 gcaacgcgcc acactgaatt ttgaaacgct gctggaagta accaacgccc tgaactcgca
       61 acgggacatc gacagtctct ggcgcgtcat cgccgatcaa attcgaaaag tgatcccttg
      121 ggatagggcc gggattacgc tctacgaatc acagaccgat tcctttcgct tttatgccgt
      181 catcaccaac atggctgctc cggccctggc gcacgatagc gtgattccac gcgagggcag
      241 cgcagtcggt tgggtccatg ataaccgtcg cctgcatatc agagaggatc tccagaaaga
      301 acaagtcttt ctggaggatc ggtattacgt gcaggaaggt ttgggacgaa tgatcaatct
      361 gcccttgctc gtccgggaac attgcctcgg cacgttgaac atcggcagcg ttcagaccgg
      421 gcatctcgat caggacgatt gtaaattcct ccagcaagtc gccacccaga tcgcatacgc
      481 gatcgaccac gtattggcat acgagcagat ccagcagctg agtgaccgct tgcgtcgaga
      541 aaatgagtat ctcgcagaag aggtcaaagc cagccgcaat ctgcgcctgg ttgtgggaac
      601 ctctttgtcc ttcagaaagg tggtcgatct tttgaaggct gtcgccccga cagacacgac
      661 ggttctcctc ctcggagaaa ccggcactgg caaagaagtc ctggcccagg ctcttcacga
      721 tttgagcctc cgcagccaca agccctttat tcgcgtcaac tgtgcagccc tgccatcagg
      781 actcattgaa agtgagttgt ttggccacga gaggggcgca ttcaccggcg cgcagctacg
      841 aagagccggg cgatttgaac tggcccacac cggcaccttg ttcctggacg agatcggtga
      901 aatgccgttg gaaacccaag cgaagttgct ccgagtgttg caagacggta tggtcgatcg
      961 tatcggagga acccaaccca tcgcagtgga cgttcgactg gtcgctgcca ccaacgccga
     1021 cctccaggcc gccgttcgac aggggacgtt tcgcgcagac ctgttctacc gccttcatat
     1081 tttccccatc gccctgccgc ccttacggga tcggcgggaa gacattcacc tgcttgcgca
     1141 gcatttcctg gcgcagatcg ggggcaagct caagcgggca catctcgcct ttgatccaca
     1201 atcgatggcg cggctcctca cctacaactg gcccggcaac gtgcgtgagc ttcaaaatgt
     1261 gatcgaacgc gccgttattc tttccggctc ctcccaagtc accgtagacg agatgttgct
     1321 cccggtcgtg caggccgttc acagcccctc cagcagccag ccagtcagct tgggggacct
     1381 tgagcgtcac cacatcatcg aggtgctgga acggacgaaa tggcgaatct atggcgagca
     1441 gggggccgcg catctgctgg gcctcaaccc cgaaacgctg cggagccgcc tgagaaaact
     1501 tgggctgcga cggccggctg gcatatcgcc ggaacctacg gctctccctt aacccatgga
     1561 gattgctgga tcgacggtgg aacctgccct tgtcaaattc ccactctgcg acgcggtatg
     1621 acaacgcgcg ctccagccga agccaggtca tgcttccgcc aatcagctca tttgaccgga
     1681 ctatggaaag catgataaat tgctgaaccg tacgagcccc gagcgaaatc ccacgcttcg
     1741 cgtttccgcc cctatcacgg cgcgatcgct tccttaactt acgctcctcc tacggcccat
     1801 ttcccccatt cagcggacaa ggaaatgggc aaagcagtgg tccgaaccgc atgcccgacc
     1861 cactgcaacc agtttgatgg cctggacatc gccatcctgt tgttcgccga aaggagtgta
     1921 cgatgaaaat tacgtccgtc tggcgcggca acaaacccaa tcacaagaaa aagcgcacgg
     1981 tcccgaagct cagaatccgt tggacacagc tcggactcgt cgatccctct gtggactcgc
     2041 cggagacgcc gatggatgaa atccgtcgtg cggtcacgaa cttggccagt tcagggttcg
     2101 gtgcggagac ccgtgcccgc tcagaacaga cccaggcacg ccctgcgcgt gcccatgccc
     2161 gtaccagtgc cactcgcacc actcgtcgac cgtccggatc ataaccaggc aggcctattc
     2221 gatcaccgat cagggaagac gaatagaagg agggaaccat cgcacgagca ggcaaaacca
     2281 ccatcgcgaa acgcgatcga gaaaaagcga agcaagtgaa gcagcgcgaa aaagaagccc
     2341 gtcgagtcca gcgcaaagcc gaaaaacccg agcgcacggt gacatcgggg ggagatgatc
     2401 ctgacctgga agggttgcag tggggccccc aggaacccct gtactgaaca ggggctcctg
     2461 ggtactcccg gaacactcgg ccggcttagg atgccttaat tacaaagcgg tcttcaccga
     2521 acacaccgcg gagttgcttg ctgaccagcg agcgatattc gcccttctcc ttggccattc
     2581 gcactaaccc ctcggcatcc gtcaggatca agagatcttc ctcggcaatc aacacccgat
     2641 ctccctttcc aacgttaatt cgatactgag tccgaccatc gggattccga ctcgggccgt
     2701 tcacaatttc agtcaatccg tcgatatgcc ccttttgccc atccaatcga tgctgtacca
     2761 tcgttccatc aggaattctg acccactgac tggcggcccg aggcgcatct tgattctgat
     2821 cgctcacctt cattcctccc ttgctacgta tgcgcggaaa aattaagact ttggcccaag
     2881 atcacttccg ttcttgattc ccccattggc ctcccgggga cctccgtcgg cgctctgact
     2941 ccacacctga gaagatccgc cgcgtcggcg tcgacgctcc ggttcgctcc gtggcgcatg
     3001 gccgtccggt cgaggcgagg cgctcatcga ggccgggctc ccctcggcgc gcggcaccgc
     3061 ctgcccgagt aaacgctcga tcgcccgcaa ttgttcctgg tcctcggacg tcacaaacgt
     3121 ggtagcgcga ccggtcgtcc tcatccgggc tgtccgcccg atccggtgca catagtcttc
     3181 gggacaattc ggcacgtcgt aattgatcac gtgggcgata tttgccacat caatgccacg
     3241 cgcggcaata tccgtggcca ccaacactcg ataggtacct cggcggaacc cttccaacgc
     3301 cgcgcggcgt tgcggcaggg tccttccgcc atggagcacc gcgacgcggt gaccggccga
     3361 atcgagcaga ttccccaatc gatccgcacg atgtttcgtc ctggcaaaca ccagcacggt
     3421 atcggactcg gactggagca acgacatcaa cagcccgttc ttacgatcgt gcgtggtgtg
     3481 gtgcacggct tgagtcacac cgtcggctgt cgtcgccgat ttcgtgacca tgacccgtac
     3541 cgggtctttc acgctggcct gcgccaatct ggcgagatcg ttcggcatcg tcgcggaaaa
     3601 cagcaacgtc tgccgctctt ccggcatggc atccagaatc tgattgatct gctgggcaaa
     3661 tcccatatct aacatccgat cggcctcgtc aaggaccaga atggacatgg caagcaggct
     3721 gatcgtgccg ttccacatat gatcgagcaa ccgccccgga gtcgccacga tgatgtcggg
     3781 gcgctgccgt agccctctca cctgcgcctg catatcggct ccgcccacca ctgttgtggc
     3841 aaacaattgc agatcgcgac ccagcgtatc gatcgtcgct tgaatctgta tcgccaactc
     3901 gcgtgtcggc gctaaaatga gcgcgcgagg ctgccctttc gggatcccgc tgagccgctc
     3961 taacatcgga atcacaaagg ccgcagtctt tcccgtccca gtctgtgcgc atcccaagac
     4021 atctcggccg gccaacgcat ggggaatggc ctgagcctgg atggccgtcg gttcagcaaa
     4081 accagccttg gtcagatttc gcaacagggt gggagacaca ccaagggagt taaaactcgt
     4141 aaacgcagtc gtctgcacgc agctgtcctt tcgttgtgga tcgcattaag acgggatcag
     4201 agaaccgagg ggagggtgaa tccggaagga gtcagctcca aactggacct ggtcgcgatg
     4261 cgatcgcgcg gtccacgttg aggaggtaca gcattggtgg accgatacaa tgctgcagta
     4321 gatcttcact gtaccgcatg gtgcccctac cggtcaacag gtccgcgagt tccggctcgc
     4381 tcccgctgac cggtcggcct gccggcaagg gtttgccgaa gagcccggat agcctatact
     4441 ggactctagc aagcgaggtc cacgatgcga atgatagcca tcctcctctt tctgtttctg
     4501 tggagtgacg ggccggtact gacggccaat gccgcttcgg aatcgtccgg caccggcaag
     4561 gccggatcca atcagggttt gacgctcgac gaaatcgggc gagggctgaa aagtgccgcg
     4621 aaaaatattg aggaggagat cccgaaaatt gggccggcta tcggcaaaac attcaagcaa
     4681 gtgaccggcg gcgaaaaaga aaaatctcca cccaagcctc ccgcgcagaa cacgagcaag
     4741 gataagaagt agcgctgacg cgcagcagga ggctgcatga gacggttgat ctctatcgtg
     4801 cttctactgt ccgcactgat acagaccggt ggctgcctct ctgctcgggc accggcctgt
     4861 ctaccgggcg agcatcctgc ggtgcttgag tcgctgtatt tcgggacgac caagcccggt
     4921 ggaaatgtca gtcctgagga atgggcggcc ttcgtcaagg acatcgtcac accagcattt
     4981 ccggaaggcc tgacctcatg gcaggcgtcc ggtcagtggc aaaagagtac cggcgcgatc
     5041 gaatacgaga cctcacgcat tctgcaacta actcacgaac agaatatggc caaagaagcc
     5101 gcgatccgac gcatcatgat catctaccag cagacctttc agcaagatgc agtcatgcga
     5161 gttcgctctc cggcatgtct ctcattcaaa gggtaactcg tgctcgctcc acgttcctac
     5221 cgatctttct tgccatggcc ccttcccccc cttccaactt cggtcaagat ttcccgaagc
     5281 aggttgcggc tgttcaaacc tcctgattgc gcaggcccta aactcccgac accggcgctt
     5341 tgaacgggag gcactgccgg cggaggtaag gctgccggca caccggtgga tgttccagtt
     5401 acagaagcag ggctggacgc ttggctgagc ggaaccaccg gaagcccgat actggttgcg
     5461 agaatagtcc ctttgctcga gggaccgcga agctctttct cccctccggc ggaagaagct
     5521 tgcagaatct cttgatccaa ttcaagtgcg tgcccgacct gggttccgac gatttgccgc
     5581 gtgagaggac tgggaaggac tcgatgctcc tccctgacgg caagaggctg aagaatgtgg
     5641 gagctttctt ggaactcgga gagagaaccg accatccccg ttctcaatga gacctccctc
     5701 cctgccttaa ggggaaacgc cttgcggtct ccggtcaatg gctcaacacg agcctgcccc
     5761 tccaacaccc gcaacgtatc gcccggaggc gcggtgacca gccgcgccaa aagtgactgt
     5821 cgctcttccc ccactaccgt ggcttccaca atgccgccac ggctgacggc acgcgccagc
     5881 ggtgtccgga gcgtcagagt atcggtcatt ctccccgcgt tgtaagacaa tgcgaccctg
     5941 accttaccct ggtgcaacaa gagttccgtt tgtccacggt gcaattcctg aacgttcaac
     6001 cgcgcgcctt cattcaacgt cagcactgca cgacgatccc acagcagggt cacttgctct
     6061 cctgttccaa cccgcaactc ctcccccgca gagatgacgt caccgacgct caaaactccg
     6121 ctcgaggtgg tggcaggaga aacgatcctc accgggattc cgacgatccc aattacgacc
     6181 cctctcgcct gaccttcacc ggacagaggc agcggcggct cctcggccca ggccatcgtt
     6241 gacatgctta caagaagcat cacgctgtag ccgagaactt gcgagcgtat atcgacgatt
     6301 ctcatcggac cacactccct ggaatattcc tgttcaagaa cgggccaaaa cgaacactca
     6361 cggttcaacg catatcctgt aaatcgttta cagcagccac tttgcggcca tgctgactcg
     6421 gaaaacagac ggacagtggc gcatagtagg tggctcgtcg atcgcaggtc aagcggccgg
     6481 catgcaggag aaattgcgga gcccggaacg gggaaccaat gtgatgatat gcacgacaca
     6541 gcagagaggc agaacttcga tcgacacgca cccgcagttc agccttgttc ttatgatgat
     6601 cctcatcctg atggcaatgg ccgtgccacc tggctccgcc caggctcaag cagccgatgc
     6661 ggaggtattg gttgccgaag gaatcttggc ctacgaggcc aaacgttatg acgaagctgc
     6721 cgtcc
//
LOCUS       DAORQN010000073         6542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_529_length_6542_cov_52.424387, whole genome shotgun sequence.
ACCESSION   DAORQN010000073 DAORQN010000000
VERSION     DAORQN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_6542_cov_52.424387"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK614_05700"
     CDS             <1..194
                     /locus_tag="PK614_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81739.1"
                     /translation="GGLLAWFIAGIEKVSFGAGAGAAMGGFLGVQISMLLDVRAARKA
                     AALEASSDEASADSAVIKS"
     gene            210..1124
                     /locus_tag="PK614_05705"
     CDS             210..1124
                     /locus_tag="PK614_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB81740.1"
                     /translation="MENRGVLIGSIIFVFGSFILMIGGLVYESYKAKQLRQQVLSITT
                     EVKPVAVAAAQDFSMYKTLVGDDGRDMVQVSEGPFVMGSRDNDSDPDEKPEHQVYLKA
                     YFLDRHEVTQDAYDRFARMTKRIKRKIEVFEEDPAKLLKPEYPMIAVTWDDAEAYCKW
                     AGKRLPTEAEWEKAARGEGKRRYAWGDEFVVGYANIDGNEDGFRYLAPPGSFESGRSP
                     YGIYDMTGNVGEWVADFYDENSYRGSPYRDPKGPDQGEQRVIRGGSWRETKRNVRSSK
                     RFQAKPWRHDITVGFRCAKDIEADVVVK"
     gene            1136..2089
                     /locus_tag="PK614_05710"
     CDS             1136..2089
                     /locus_tag="PK614_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB81741.1"
                     /translation="MLETRFKVIFLLVVLFAASLPVIAILRGTVSTPFEAEPRPAEQD
                     QGASNPELASAEEPLEEELVVIPAGPFIFGTNRGGFDEQPERRIYLDQFLIDRYEVTN
                     AQYAAFVKATGHRKSGPPSRYAKNTLRMRGVNQPAVYVSWEDAKAYCEWRGRRLPSEA
                     EWEKAMRGPDGRLWPWGNVEVPNAANWARVDDGFEVAAPVGRVKSDASPYGVMDGAGN
                     VMEWVNDWYLEGAYAAASERNPESPEYGTYKVLRGAAYTSSGSDLRITARSKMMLDFR
                     DETIGFRCAQSSAENGRSSGGVGPEKIIGNRSSRGSETRPK"
     gene            2602..4521
                     /locus_tag="PK614_05715"
     CDS             2602..4521
                     /locus_tag="PK614_05715"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB81742.1"
                     /translation="MGSVTRTKLMSIMALGAMIGLLLLPILVAIPALASDGGAPAAGV
                     KKEGGDAKVEKGKDVYYKTEGIVSGPPAPKTTDSEKDYPRYNFESRVLLWFANQQHLY
                     YGSFVLAVPIFCMVIEFMGVVTKDKAMAKRYDQLAYDFIKISLTAYSLTAILGGILIF
                     TFLTLYPAFFQYLSGIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMS
                     VVLNVIGTILMFLANSWIGFMMSPAGVDEQGRYLGNIWHVIHTALWNPLNLHRILGNM
                     AFGGGVVAAYAAYRFLSAKTDEERAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYA
                     YRQQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQALGRMRGAEKYQRYIKYLVF
                     LLTCGYLVFITPHTIVMTPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFV
                     WYMRGNKVSTVSWSKFGNIFMGCFFFFAYLNIIMLAVYGYYIPANVRVGLSVPQVATT
                     LSCLFFMFALNSVMMKGAKQMGPIEWGKISARSQYALIMLATAFTWMMGLMGYIRSSV
                     RLFWHVNEIMRDNSPWAYTHTVGFAANMISANVLFFWISIMFVFWLGALGAKKAPAEA
                     KAAVPGRAAAPAVGH"
     gene            4607..5920
                     /locus_tag="PK614_05720"
     CDS             4607..5920
                     /locus_tag="PK614_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HRB81743.1"
                     /translation="MIELIKAAVAMGWPALGILVALMFYFKASISDPVANKRAVFKTF
                     IGTIGAMLLFMAIANYKMNFFEESRLLPVSLVLITSMTFMMALYFTNISALLKIGGFM
                     FFIAAALSGYGNWLPQVEGGFPPIEEKKDFSNMPQTELADEGEKIIFGGVGQNKVQGA
                     IGKGQCPLCHAFHKGMLGERAPNLDGLPERAGTQIEDPRYHKGNAAARDSDQKEAFPG
                     SGTAENGQEYIAESHACPSCFVVAGYGVKGTNDKVSPMPSIHKPPISLSLPELAAVDT
                     WLYMREGRDAPGFDEIVKSYEKFIPESDRPKPPTEGDAKPGASALMADGTEPVDQIFA
                     KGQCVACHTIPGIAGATGTIGPKLVEGTNAPLRIKDKDYKGKAKSVPDYIMESIVEPS
                     AYVVKGFPDNTMPKVFGQKLSAGALKKLVDYLSQVQEGKEPPKAS"
     gene            5969..>6542
                     /locus_tag="PK614_05725"
     CDS             5969..>6542
                     /locus_tag="PK614_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB81744.1"
                     /translation="MKALMSVVALIGVLGLLMLIGMIFGVVPSNTVRLVEGYMPMQVL
                     SELAIFVAGFTGLSYLANSMGIAFPRFWQGVLFWAFIQAYLKFRIYPPIPFSVRAMYG
                     TVSFVAVFMWVSANEEDWKKFRQPILNVLDANTGFHKALRTMYLILLPILIGGFSFMT
                     MKPSVDEPIELRTVHPAPPASTKVHGKTYTL"
BASE COUNT         1413 a         1502 c         1961 g         1666 t
ORIGIN      
        1 tcggtggcct gttggcgtgg tttattgccg gaattgagaa ggtctcgttc ggggcagggg
       61 ccggcgcggc gatgggggga tttctcggcg tgcagatttc gatgttgttg gatgtgcggg
      121 cagcgagaaa ggctgcggca ctggaagcga gtagtgacga ggcttcggct gactcggcgg
      181 ttattaaatc ctgaggtgcg agtaacgaga tggaaaatag gggtgtgctg atcgggtcga
      241 tcatctttgt gttcgggtcg tttattttga tgattggcgg gctggtctac gaatcctata
      301 aggcgaagca gttgcgccag caggtgcttt cgatcaccac ggaggtcaag ccggtcgccg
      361 tggccgccgc gcaggacttc tcgatgtata agacgcttgt cggcgatgac ggccgtgaca
      421 tggtgcaggt ttctgaaggg ccgtttgtga tgggtagccg tgacaacgat agtgatccgg
      481 atgaaaagcc tgagcaccag gtatatctga aagcatattt tctggatagg cacgaggtca
      541 cgcaggatgc ctatgatcgc tttgccagga tgacgaagcg aatcaagcga aagatcgaag
      601 tgttcgaaga ggatcctgcc aaattgctga aacctgaata tccgatgatt gctgtgacgt
      661 gggacgatgc agaggcctat tgcaaatggg ccggcaagcg gttgccgacg gaagcggagt
      721 gggagaaggc cgctcgcgga gaagggaagc gtcgctatgc ctggggtgat gagtttgtgg
      781 tgggatacgc gaacatcgat ggaaatgagg acggatttcg ttatctggct cccccggggt
      841 cgttcgagtc gggacgcagt ccctacggaa tctacgatat gacgggtaac gtcggtgagt
      901 gggtcgcgga tttttacgac gaaaattcgt atcggggctc tccgtatcgt gacccgaagg
      961 ggcctgacca gggcgagcag cgtgttatcc gtggcggttc ctggcgggaa accaagcgaa
     1021 atgtgcgttc gtcaaagcga tttcaggcaa aaccgtggcg acatgacata acggtcggct
     1081 ttcgctgcgc gaaagacatc gaggccgatg tggtcgttaa gtagtctgta tcggcatgct
     1141 cgaaacgcga ttcaaagtga tttttcttct ggtggtgctg tttgccgcca gcttgcccgt
     1201 cattgcgatt cttcgaggga cggtatccac tccgtttgaa gctgagcctc ggccggccga
     1261 acaggatcag ggagcctcca atcctgagct ggcctccgct gaggaacctc ttgaagaaga
     1321 gctcgttgtg attcctgcgg ggccgtttat cttcgggacc aaccggggcg ggtttgatga
     1381 gcagcctgag cggaggattt acctcgacca gtttctgatt gatcgctacg aggtgactaa
     1441 cgcgcaatat gccgcgtttg tgaaggcaac ggggcatcga aagtcaggac ctccctcgcg
     1501 gtatgcgaaa aatacgctgc gtatgcgagg tgtgaatcag cctgcggtct atgtctcctg
     1561 ggaggatgcg aaagcctatt gcgagtggcg tggaaggcgg ttgccttctg aggcggagtg
     1621 ggagaaggcc atgcgcgggc ccgatggtcg cctgtggcca tggggaaacg ttgaagtgcc
     1681 gaacgcggcg aattgggctc gcgtggacga tgggtttgag gtggcggcgc cggtggggcg
     1741 ggtgaagagt gacgccagtc cctatggggt tatggatggt gcaggcaatg ttatggaatg
     1801 ggtgaacgat tggtaccttg agggggccta tgctgcggct tcagagcgca atccggaaag
     1861 tcctgaatac ggaacctata aggtgttgcg cggggctgcc tataccagtt cggggtctga
     1921 tttacgcatt accgcgcgca gcaagatgat gctggatttt cgggatgaaa caatcggctt
     1981 tcgttgcgct caatcaagtg cagagaatgg ccggtcgtct ggaggcgtgg ggccggagaa
     2041 aatcatagga aatcgaagta gtagaggatc agaaacacgg ccaaaatgat attgacaacc
     2101 attccagcca aaactataat gtcgaacact tttgaatttt tagccataaa aatcccttta
     2161 aaaatcatgt gcgtgaggga ttttgattaa cacagcgtag ttggtgtgtc aattcaaaat
     2221 acgtacttaa accgttggga gaatcaagat ggaagctgct caggatgacg tccaagttaa
     2281 gatcggaaaa atgattttct acatcaccct ggcagtgagt ctttggtttt tttactggtt
     2341 cggcgggatt cagtgccctt gctgatccgg tattctggcc tgggaaattg tttatcaagt
     2401 tgaggggggt ttgagatggg ccaaacaatt gtgtatgtcg tcgtatccgt tttgatctgc
     2461 gtctcgtttt ttgcagcggt tgatcatttt ctgatggacg cgcagggttt ggacttttgg
     2521 tatctccttc gaaagtaatc attcggaggg tagggctttg aagttgttgt tcacatacgc
     2581 gatgtatcaa ggaggtaacc catgggctct gtaacccgca cgaagttgat gtcgatcatg
     2641 gcgctaggcg caatgatcgg cctcctcctc ttgcctattc tggttgcgat ccctgccctt
     2701 gctagcgacg gtggcgctcc tgctgccggc gtgaagaaag agggtggtga cgcaaaggtt
     2761 gagaagggta aggacgttta ttacaagacg gaaggtattg tctcgggccc accggctcct
     2821 aagacaacgg acagcgagaa ggactatcct cgatacaatt tcgagagtcg cgtcctgctc
     2881 tggtttgcca accagcagca cctttactat ggtagcttcg tcttggctgt tcctattttc
     2941 tgtatggtca tcgaatttat gggggtcgtg accaaagata aggcgatggc taagcggtat
     3001 gatcagctgg cgtacgactt tattaagatc agtctgacgg cgtactctct gacggccatc
     3061 ttgggcggaa ttctgatctt taccttcctg actctctatc cagctttctt ccagtatctc
     3121 tcgggtatct ttaggccggt catgcatatt tatgcattga tgttcgtggc cgaaagcgga
     3181 accctctaca tttattatta tggctgggac aagatgaagg aggggttcct gaaatggatt
     3241 cacctcagca tgtccgtcgt cctgaacgtc atcggtacga tcttgatgtt cctggcgaat
     3301 tcatggatcg gcttcatgat gtctcctgcc ggcgttgacg agcagggccg gtatctcggt
     3361 aatatctggc acgtgatcca caccgcgttg tggaacccgc tcaatctcca tcgcattctc
     3421 ggtaacatgg ccttcggtgg tggtgtcgtg gcggcctatg cggcctatcg gttcttgtca
     3481 gctaagaccg atgaggagcg tgctcactac gattggatgg gttacatcgc gatggctctc
     3541 ggtgtggcgt tcctgattcc gttgccattc gcgggttatt ggttgatgcg tgaggtctat
     3601 gcctatcggc agcagatggg tatcaccttg atgggcggat tgttggcttg gttgttcatc
     3661 attcaggcca ccatgatcgg aattctgttc ttgagcacca actactatct ctggcaagcg
     3721 cttggccgga tgcgtggtgc ggaaaaatat cagcgctaca ttaagtatct cgtgttcctg
     3781 ctcacgtgcg gatacctggt ctttattacg ccgcatacca tcgttatgac tcccgccgag
     3841 ttgaaggcga tgggtggcca gcagcatcca gtgctgggta actatggtgt gatgtcggcc
     3901 aaaaacggcg gcatcaatgt cattatcacg acaaccgtgt tgagcttcgt gtggtatatg
     3961 cgaggcaata aggtttcgac ggtgtcgtgg tcgaagttcg gcaatatctt catgggctgt
     4021 ttcttcttct ttgcgtacct caacatcatt atgttggctg tgtacggcta ctatattccg
     4081 gcgaacgtac gcgtcggctt gtccgttccg caggtggcta ctacgctgtc gtgcctgttc
     4141 ttcatgttcg cgcttaacag cgtcatgatg aagggtgcca aacagatggg accgattgag
     4201 tggggtaaga tttctgcccg gtctcaatac gcgctcatca tgttggccac ggctttcacg
     4261 tggatgatgg gtctgatggg atacatccga tcctctgtcc gcctgttctg gcacgtcaat
     4321 gaaattatgc gtgacaattc accttgggcc tacactcata cggtgggctt cgctgcgaac
     4381 atgatttcgg ccaacgtgct gttcttctgg atttcaatta tgttcgtgtt ttggttaggg
     4441 gcccttggtg ctaagaaggc accggccgag gcgaaggcag ctgtgcctgg cagggcggca
     4501 gcaccagcag ttggtcacta atcaaagcgc aagaaatgaa tcaatgaggg agggtgtctg
     4561 gctatcgcgt agccagagcc ttcccctcct gcaggaggtt tgagctgtga ttgaactcat
     4621 taaagcggcc gtagcgatgg gctggcctgc attagggatt cttgtggccc taatgtttta
     4681 ttttaaggcc tccatttcgg atcctgttgc caataagcgt gcggtattca agacattcat
     4741 cggcactatt ggcgccatgc ttctttttat ggctatcgcc aattacaaga tgaatttctt
     4801 cgaagagtcc aggcttttgc ccgtgtcttt ggttctgatt acgtctatga cgttcatgat
     4861 ggcgctctat ttcacgaata tcagcgcctt gctgaaaatc ggcggattca tgttcttcat
     4921 cgccgccgct ctctcaggat atgggaactg gttgccccag gttgagggtg gatttccgcc
     4981 catcgaagaa aagaaagact tcagcaacat gccccagacg gagcttgctg acgagggcga
     5041 gaagattatt ttcggtggag tcggtcagaa taaggttcag ggcgcaattg gtaagggcca
     5101 gtgtccgctc tgccatgctt tccacaaggg tatgttgggc gagcgcgcac caaacttaga
     5161 tggactccca gagcgtgcag gaacccagat cgaagatcct cgctatcata aggggaacgc
     5221 tgctgctcgc gattccgatc aaaaggaagc atttcctggg tcaggaacgg ccgagaatgg
     5281 gcaggagtat attgctgagt cgcatgcgtg tccaagctgc ttcgtggttg ctggttatgg
     5341 cgtgaaggga accaatgaca aggttagccc gatgccgtct atccataagc caccgatctc
     5401 tttgtctttg cctgagctgg ccgcggtaga cacctggtta tatatgaggg aagggcgaga
     5461 cgcccctggt tttgacgaga ttgtgaaatc ctatgaaaag ttcatcccgg aatcggatcg
     5521 tccaaaacca ccaacagaag gtgatgcaaa gcctggcgcc agcgccttga tggcggacgg
     5581 aaccgaacct gtcgatcaga tttttgcaaa gggacaatgt gtcgcgtgcc atacgattcc
     5641 tggaatcgct ggagcgaccg gcacgattgg tccgaagttg gtcgagggaa cgaatgctcc
     5701 actgcgcatt aaagacaagg attacaaggg gaaggcgaag agcgttcctg actacatcat
     5761 ggaatccatt gtcgagccta gtgcttacgt tgtgaaggga ttccccgaca acaccatgcc
     5821 caaggtgttc gggcaaaagc ttagcgccgg cgccctcaag aaacttgtag attacctctc
     5881 gcaggtacag gaagggaaag agccacccaa ggcctcctaa ctgcgatggg tatctggttg
     5941 ctactgcgga agaaagggag atgaggacat gaaagcatta atgtcggtcg tggcattgat
     6001 aggcgtcctg gggttgctca tgttgatcgg aatgatcttc ggagtggtgc cctcgaacac
     6061 cgtcaggctc gttgagggct acatgcccat gcaggtactg agtgagctgg ccatcttcgt
     6121 ggctgggttc accggcctga gttatctagc caactcaatg ggaattgcct tcccccggtt
     6181 ttggcagggt gtgttgtttt gggcttttat ccaagcctat ctcaagttcc gtatctatcc
     6241 accgattcca ttcagcgttc gtgccatgta cggaaccgta tcgttcgtag ccgtattcat
     6301 gtgggtctcg gcaaatgagg aggattggaa gaaatttcgt cagccaattc tcaatgttct
     6361 ggatgccaac actggattcc ataaggcact caggacgatg tatctaattc tcttgccgat
     6421 tcttatcggc ggattctcct tcatgaccat gaagccgagt gtggatgagc cgattgagct
     6481 ccggacggta catccggcac cgccagctag cacgaaggtg cacggcaaga cctacaccct
     6541 gc
//
LOCUS       DAORQN010000074         6496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_536_length_6496_cov_17.741500, whole genome shotgun sequence.
ACCESSION   DAORQN010000074 DAORQN010000000
VERSION     DAORQN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_6496_cov_17.741500"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK614_05730"
     CDS             <1..266
                     /locus_tag="PK614_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81745.1"
                     /translation="SSVKKLADNAGEMGKPKSISSLRLKECEGKTEVDAPCHAAKTLL
                     NTSPKTPDGANLPLQYDVLLPNGKPLANLIPWTAKYNQGGGHH"
     gene            complement(372..3020)
                     /gene="mutS"
                     /locus_tag="PK614_05735"
     CDS             complement(372..3020)
                     /gene="mutS"
                     /locus_tag="PK614_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRB81746.1"
                     /translation="MGDADGTPLMRQYRDIKQAYRDAILFFRVGDFYEMFQEDAVEAS
                     KLLSIALTSRDKSSETPIPLCGVPYHAATNYIAKLLRAGRTVALCEQVEDPKLAKGLV
                     RREVVRLYTPGTLIDSEFLPSTESNVLAAVCVRLRSAGIGRKEPSYGLATLEVSTGDY
                     WLSEFHGIQAEASLRDELARLEPKELLFPEDLSPDEQQWTADLTGPRCCAQPATWFDL
                     EQGRMTLQEQFRVHSMEAFGCHRLTLGIQAGAAVLRYVRETQPSAPLDHIRPPRVRQD
                     HDAMHLDSVTIRNLELVRPAGRAEESSNQSTYTLLGVLDRTVTAMGSRLLREWLLRPL
                     VNSAAIEARLTAVDELKRQIDARVRLRTALRTVQDISRLCSRMSLGVANPRDVLALKI
                     SVSSLPAIQAELSGLDSPLIADLMSSWDNAQDLYELIEGAIEQEAPVSIRDGGILKSG
                     YHPEVDELRKASREGKGWIAGLEAKERERTGVESLKIRYNQVFGYYIELTKANLGKVP
                     PDYIRKQTLVNAERFMTAELKELEERVTGADTKLTALEQALFEQLRTRLAEETARLQE
                     ISRRLATLDVVASLAETAALNRYVRPTIDEGEGLHIVQGRHPVVERLDLSGGFIPNDT
                     HLDLATSRLHILTGPNMAGKSTYLRQVALITLMAQMGSFVPATEARIGLTDRIFTRVG
                     ASDNLAGGQSTFMVEMTESAHILNCATPRSLILLDEIGRGTSTYDGLSIAWAIAEYIQ
                     DPQRLGARTLFATHYHEMTQLESLREGITNYCVAVQERDGKVLFLRKIIRGGADRSYG
                     IHVAELAGLPDQVIHRAKAVLAQLESSSSSGRSVQENQQSLQFDATLPAPHPILEEVR
                     QMNLFSMTPLDALNRLAELQQRLLQE"
     gene            complement(3013..3357)
                     /locus_tag="PK614_05740"
     CDS             complement(3013..3357)
                     /locus_tag="PK614_05740"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRB81747.1"
                     /translation="MIRLILVGTLLLLALSFFLQNQEQEVTLRYFFGLRSASILIYKP
                     ILAAFGVGLLVSGILLFPAWVRGRIELRRKTKALQEAEVDLERLRQALDKAARRVGTS
                     SDIPAEGEPSDG"
     gene            complement(3354..3875)
                     /gene="tsaE"
                     /locus_tag="PK614_05745"
     CDS             complement(3354..3875)
                     /gene="tsaE"
                     /locus_tag="PK614_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRB81748.1"
                     /translation="MLRSPKHTHRLGRCLGTLLQGGEILALFGELGAGKTSLVKGIAD
                     GLLAEPTDVSSPTFTLIHEYRGRLPLVHSDLYRLTAAQLEDTGLNDYLDGHTVTAIEW
                     ADRWGDGLPSDRLDVHLSHRPPAARRAILTARGPAARRLLDALRSRLSAHRPTRAAQQ
                     TRVQLSRPRRASH"
     gene            complement(3952..5508)
                     /locus_tag="PK614_05750"
     CDS             complement(3952..5508)
                     /locus_tag="PK614_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRB81749.1"
                     /translation="MIPIVTAEQMRALDQRTIAEALVPSLTLMERAGAGVVTHLEARY
                     APLAGKSVIILCGKGNNGGDGFVVGRLLRRKQARVHILLLASPEDLSRDAKTMYQRFL
                     RSAGKSAVTCPADVETIQQRLATGDILVDAILGTGLSTPVTGLYRDAIEAITAVGRPT
                     VAVDLPSGLHADTGAVLGAAVRADLTVTLGLPKAGLYSGSGIDCAGTVRLVDIGIPAS
                     FVEAIGSRLMLLTDTGARAALPPRRPSAHKGTYGHLGVIAGSVGKTGAAAMAALSALR
                     IGTGLVTAAIPSSANDILEAKLLEVMTLPMPETKARTFARSGLDRLLAFAGARDAVAI
                     GPGLTTHPETVDLVQELVKRIDKPCVLDADALNALAGKSSLLTECKRPPIITPHPGEM
                     ARLETEATTQSVNEDRLGTATRFARERGVFVILKGARTVIARPDGLAAICPTGNPGMA
                     TAGTGDVLTGMVGGLLAQGLAPWDAACAATYFHGLAGDLAAQHLGQAGMIASDLIQHI
                     PHALAPTTHT"
     gene            complement(5577..6290)
                     /locus_tag="PK614_05755"
     CDS             complement(5577..6290)
                     /locus_tag="PK614_05755"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRB81750.1"
                     /translation="MARLGVNIDHVATLRQARGGTDPDPLTAAILVELAGADGLVVHL
                     REDRRHIQDRDLTMLREIVRTKLDLEMAADDAMAKIALSVKPDLVTLVPERRQELTTE
                     GGLDVANHRDRIQKIVDLLRDGGIPTSLFVEPSLDQIKAAHKIGAAYVELHTGRYSNA
                     KRSKEEDEEFEAITQAAKLAYKLGLGVNAGHGLTYKNVKRLAKLPEIVEFNIGHSIIA
                     RSVMVGLVQAVREMRELVA"
BASE COUNT         1262 a         1828 c         2049 g         1357 t
ORIGIN      
        1 tttcgtcagt taagaagttg gccgacaatg ccggcgaaat gggtaagcca aagagcatct
       61 cctcccttcg cttgaaggaa tgtgaaggga agacggaagt ggatgctcct tgccacgctg
      121 cgaagacgtt gctgaacacg tccccaaaga ctccggatgg agcgaatttg ccgttgcaat
      181 atgatgtgtt gttgcccaat ggcaagccgc ttgccaacct gattccctgg accgccaagt
      241 acaatcaggg tggtggacat cactgagcag tagaaggcca ttggcctgaa gtaggaaggc
      301 ggcattccga aagggatgcc gcctttttat ttgcgacgtc gagggaggaa ctgtctaggc
      361 cgggatgtga gctactcctg cagtagccgt tgttgcagct cagcaagtcg attgagcgca
      421 tcgagggggg tcatggagaa gagattcatt tggcgaactt cctcaagtat aggatgaggc
      481 gcagggagcg tggcatcaaa ttgaagcgac tgctggttct cctgcactga ccgtccggat
      541 gacgaggagg actcaagttg agcaaggacg gccttggccc ggtgtattac ctgatcgggg
      601 agacctgcca gttcagcaac atggattccg tagctgcgat cggcgccccc tcgaataatc
      661 tttcgcagaa acagcacctt cccgtcacgt tcctgtaccg caacgcaata gttcgtaatc
      721 ccttcccgta gactctccaa ttgcgtcatt tcatgatagt gagtcgcgaa gagcgtgcgc
      781 gcgccgagac gctgaggatc ctggatgtat tctgcaattg cccaggcaat gctcagcccg
      841 tcataggtgc tcgtgccgcg tccgatttca tccaggagga tcaagctgcg aggtgtggcg
      901 cagttgagga tatgggctga ttccgtcatc tcaaccatga aggtactctg gccacctgcg
      961 agattgtcgg atgccccgac tctggtaaaa atgcgatcag tcagcccgat ccgggcttcc
     1021 gtggcgggga caaagcttcc catctgggcc atcaaggtaa tcagagcgac ctgtcggagg
     1081 taggtgctct tcccagccat gttggggccg gtcagaatgt gcagccggct ggtagccaga
     1141 tccagatggg tgtcgttcgg gataaatccg ccggagagat caaggcgttc gacgaccggg
     1201 tgtcggccct gaacaatatg gaggccctca ccctcgtcga tggttggtcg cacatagcga
     1261 ttgagcgcgg cggtttccgc tagggaggct accacgtcaa gggtggccaa tcgtcggctg
     1321 atctcttgaa ggcgagctgt ttcctcagcg agtcgtgtcc ggagctgttc gaacagcgct
     1381 tgctccaggg ccgtcagttt tgtgtcggct cccgtgaccc gctcttcaag ttcctttaac
     1441 tcggcggtca tgaagcgctc ggcattcacc agggtttgct tgcggatata gtcgggcggt
     1501 accttcccga gattcgcctt cgtgagttca atatagtacc cgaagacttg gttatatctg
     1561 attttaagcg attcgacgcc ggtccgttcg cgttctttgg cttcgaggcc ggcaatccag
     1621 cctttgccct cccgacttgc cttgcgaagt tcatcgactt caggatggta tccgctcttc
     1681 aggatgccgc cgtcgcgaat cgatacgggc gcctcctgct ctatggctcc ttcgatcaac
     1741 tcgtagagat cctgggcgtt atcccatgac gacatcaggt cggcgatgag tggagaatcg
     1801 agcccggaca attcggcctg aattgcggga agcgaagaga cggaaatttt cagagcgagg
     1861 acatcccgcg gattggcaac tccgagagac atgcggctac agagacggga aatgtcctgg
     1921 acggttcgca atgccgttcg gaggcgaact cgtgcgtcga tctgtcgttt gagttcgtca
     1981 accgctgtca gtcgggcctc gattgccgca ctgttgacca atggccgcag gagccactca
     2041 cggagcaacc ggcttcccat ggcggtgacg gtgcgatcca gaacgcccaa taacgtatag
     2101 gtggactggt tcgacgattc ctccgcacga ccggcaggcc tgacgagttc cagattgcgg
     2161 atggtgacgc tgtccaggtg catggcgtca tgatcctgtc tcactcgtgg cgggcggata
     2221 tggtcgagag gggccgacgg ttgagtctcc ctgacatacc gcaacactgc cgccccggct
     2281 tggatgccca gtgtgagtcg gtggcagccg aaggcttcca tggaatgcac gcgaaactgt
     2341 tcttgtaacg tcatgcgacc ctgctccagg tcgaaccagg tcgcaggttg tgcgcagcat
     2401 cgggggccgg tcagatcggc ggtccattgc tgttcgtccg gagagagatc ttcgggaaac
     2461 agcaattctt tcggttcaag ccgcgccagt tcgtcccgca gggaggcttc tgcctggatc
     2521 ccatgaaact cactgagcca atagtctccg gtcgagactt caagcgttgc gaggccatag
     2581 gagggctctt tgcgtccgat ccctgcggat cgaaggcgca cgcataccgc ggccagcacg
     2641 ttggattccg tggacggaag gaattcgcta tcgatgagtg tgccgggggt atagagccgg
     2701 acgacttccc tgcgcaccag acccttggcc agtttggggt cttcgacttg ctcgcacagg
     2761 gcgacggttc gtcccgcacg gaggagtttg gcgatgtaat tagtggcggc gtgatagggg
     2821 acaccgcaga gagggatcgg ggtttcgctg gatttatcgc gtgatgtcag cgcaatggag
     2881 agaagtttgg acgcctccac cgcgtcctcc tggaacattt catagaaatc gccgacacgg
     2941 aaaaaaagga tggcatcgcg ataggcctgc ttgatgtcgc gatactgccg catcaatggg
     3001 gtgccgtcgg cgtcacccat cggagggctc tccctcagcg ggaatgtccg aagacgtccc
     3061 cacgcggcgg gcagccttgt cgagggcttg tcggaggcgc tccagatcca cctcggcctc
     3121 ctgcagcgcc ttggttttgc gccggagttc gatgcggcca cgtacccaag ccggaaacag
     3181 caggatcccg gacactaaca gtccgacgcc gaaggccgcc agaatcggtt tatagatcag
     3241 gattgaggcg gatcgaagac cgaaaaaata ccgcagagtg acttcctgct cctggttctg
     3301 caaaaaaaac gacagcgcga gtagcagcag cgttcccacc aatatcaatc ggatcaatgc
     3361 gaggccctcc ttggccggct caattgtaca cgggtttgct gagccgctct agtggggcga
     3421 tgtgcggaga gccgggagcg aagcgcgtcc agcagacgcc gtgcagcagg gcctctggct
     3481 gtgagaattg cgcggcgggc cgccgggggg cggtgcgaca agtggacatc gagccggtcc
     3541 gacggaagac cgtcgcccca ccgatcggcc cattcgatcg cagtgacagt gtggccgtct
     3601 aggtagtcgt tgagtccggt atcttcgagt tgggcggcgg tcaggcggta gaggtcggag
     3661 tggaccaatg gaaggcgtcc ccggtattcg tggatgagtg taaacgtcgg gctgctcacg
     3721 tccgtcggtt cggcgagcag cccgtcggcg atgcctttga ccagtgaggt cttgcctgct
     3781 cccagttcgc cgaacagggc gaggatttcg cctccctgga ggagtgtgcc caggcaacgt
     3841 cccaggcggt gcgtgtgttt cggagatcga agcacaatgc gccacggttt gccgataggg
     3901 ggagaccccg cggttgtttg cctgagaggt gccgcaggtg atggcaccgg cttaggtgtg
     3961 tgtggtcggc gcgagggcat gggggatgtg ctgaatgaga tcactcgcaa tcatgccggc
     4021 ttggccgaga tgctgcgcgg ccaaatctcc ggccaaacca tgaaagtatg tcgccgcgca
     4081 ggcggcgtcc caaggggcca agccttgcgc gagtagcccg ccgaccatgc ccgtcaagac
     4141 atcaccggtg ccggcggtgg ccattccggg attgccggtc gggcagatgg ctgccaggcc
     4201 atccgggcgc gcaatcaccg tgcgggctcc tttgaggatg acaaagacgc cgcgttcccg
     4261 cgcaaatctg gtggccgtgc caagacgatc ttcattgacc gattgcgtgg ttgcctcggt
     4321 ttccaacctg gccatctcgc ctggatgagg agtgataatc ggcggtcgct tgcactcggt
     4381 cagtaacgag gatttccccg ccaatgcatt caacgcatcg gcatcgagta cgcagggctt
     4441 gtcgattcgt ttcacgagtt cctgtaccag gtcgacggtc tcgggatggg tggtcaggcc
     4501 cggtccgatc gcgacggcat cccgggcgcc ggcgaacgcg aggaggcgat ccaaaccgga
     4561 acgcgcgaag gtccgagctt tggtttccgg catcggcagt gtcatcactt cgaggagttt
     4621 tgcttccaga atatcattgg cgctcgaggg gattgcggcc gtcaccaagc cggttccgat
     4681 gcgcagggct gagagtgctg ccatggccgc cgcgcctgtc ttgccgaccg agccggcgat
     4741 gacgccgagg tggccgtagg tgcctttgtg tgccgagggg cgtcgtgggg ggagtgccgc
     4801 gcgagcaccc gtatcggtca gcagcatcaa ccgacttccg atggcttcga caaaggaggc
     4861 gggaatccca atatccacga gtcgaaccgt tccagcgcaa tcgatgccgg atccactata
     4921 gagacccgcc ttcggcaatc ccaacgtcac cgtgagatcc gcgcgcacgg cggcccccag
     4981 caccgctcct gtatcggcat gcaggcctga tggaagatcg acggccacgg tcggtcggcc
     5041 gacggcggtg atggcttcga tcgcatcacg atagagcccg gtgacggggg tggagaggcc
     5101 ggtaccgaga atcgcatcga cgagaatgtc cccggtcgcc aaccgttgtt ggatcgtctc
     5161 cacgtcggcg gggcatgtaa cggccgattt ccctgccgat cggagaaatc gctggtacat
     5221 cgtctttgca tcgcgactca gatcctcggg ggacgcgagc agcaggatgt ggacccgggc
     5281 ctgttttcgt cgtaacagcc gtccgacgac aaaaccgtct ccaccattgt tccccttgcc
     5341 gcagaggatg atgaccgatt tcccggcaag cggtgcatat cgggcctcaa ggtgagtgac
     5401 gacccccgct ccggctcgtt ccatcagcgt cagggacggc accaaggctt ccgcaattgt
     5461 ccgctgatcc agggctcgca tttgctcggc ggtcacgatc ggtatcatgg tgttctgtga
     5521 ctcccggtcg gtttctcgca gcgagggtga cagcccgtgc cgtgggggtg gccggactag
     5581 gcgaccaatt ccctcatttc gcgcacagcc tggaccaatc caaccatgac ggatcgggcg
     5641 atgatgctgt gaccgatgtt gaattccaca atctcgggca acttggcgag tcgtttcaca
     5701 tttttatagg tcaagccatg cccggcgttg acgccgaggc cgagcttgta ggccagtttg
     5761 gctgcctggg tgatggcctc gaattcctcg tcttcttcct tcgatcgctt ggcgttggag
     5821 tagcggccgg tatgtaactc gacataggct gcaccgattt tgtgcgccgc cttgatttga
     5881 tcgagactcg gctcgacgaa caggcttgtg gggatgcctc cgtcgcggag caggtcgacg
     5941 attttctgaa tgcgatcgcg gtgattagcg acatcgagac cgccttccgt ggtcagctcc
     6001 tgtcggcgtt ccgggacgag tgtcaccagg tccggtttga cgctcagggc gatcttggcc
     6061 atggcgtcgt ccgcggccat ctccagatcc agtttggttc gcacgatttc ccgcaacatg
     6121 gtgagatctc gatcttgaat atgccggcgg tcttcccgaa gatgcacaac caggccatcg
     6181 gcgccggcca actccacgag aatggccgcg gtcaaggggt cgggatcggt ccctcctcgc
     6241 gcctgccgga gtgtcgccac atgatcgata tttacaccca gtcgagccat tacgcctccg
     6301 caatttccct gcatgtcata ggagaaagat ttcggaactg gtcgaaaatc atagacaatt
     6361 ctctcgttac tgaggcatta ttaacagaag acggtacggg ggagcaagga ggcagatcgt
     6421 gggaggccgc actgttttga gtgtgaccac gatagggtgc tcgcccccaa gtcctgacaa
     6481 tcgtagcgaa attgac
//
LOCUS       DAORQN010000075         6451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_540_length_6451_cov_14.839431, whole genome shotgun sequence.
ACCESSION   DAORQN010000075 DAORQN010000000
VERSION     DAORQN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_6451_cov_14.839431"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PK614_05760"
     CDS             complement(<1..960)
                     /locus_tag="PK614_05760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRB81751.1"
                     /translation="MHISVIGTGYVGLVTGACFAEFGVNVTCMDTDARRIARLEKGEV
                     PFFEPGITELVAKGIKEDRLHFTTDVAKAVDKALVIFIAVGTPPKSDGSADLSYVEEV
                     GRGIAKNMTGYKVIVTKSTVPVGTGEKLREVIKANQTGRFRFDIVSNPEFLREGSAIE
                     DFMRPNRVVIGADSEQAVAIMKDLYRPLYLLETPIVVTDIPTAEMIKYASNAFLAVKI
                     SFINEIATVCEKVGADVQMVSKGMGLDNRIGNKFLHAGPGFGGSCFPKDLAALVQTGE
                     RVGYPFQIAGAAAKVNYEQHLRMVEKVKEACGGVKGKTLGVLGL"
     gene            1190..1561
                     /locus_tag="PK614_05765"
     CDS             1190..1561
                     /locus_tag="PK614_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB81752.1"
                     /translation="MMMRPITRTVHPDDTLLAVARQLRDARVGAMLVADHGDYVGIVS
                     EADLVRKAMASGADAEQVMARSVMSTPVMTIDIAQSAHEASDVMAERGIRHLVITEEG
                     RVVGMISVRDLLRYFKNWGTL"
     gene            1655..3199
                     /locus_tag="PK614_05770"
     CDS             1655..3199
                     /locus_tag="PK614_05770"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRB81753.1"
                     /translation="MPRLFLLITALVTGAIVMALEILGSRLLAPVFGNSLFVWGALIG
                     IILAAMSSGYAFGGWASDRYRVAAVLAWLLLGSGAWTLLMSWTGQVAIFKVAKLVEDP
                     RWGPSLAACVLLAPPAFGLSGVMPALLRLAVVDMGYLGRHTGSMIALSTVGSLAGTWG
                     TAFFLLSWLGTQTLVASLGVLQLLLGLLWLQQGSGKVTKVTGLLLAGLLILSWQLFGQ
                     AAPVAGLVYQEDSPYQQVRVRDDDLFRYLVLDRTWHAVMWRSDPATLFLPYSQLMVAA
                     VALTPDPKRGLILGHGGGSLAKWLAQVWPELELDVVEFDPVVVRMAQEYFEYHPPANH
                     HVFVKDARVFVRDTEVKYDVIWVDAFARHLIPFHLTTVEFFSELRSRLNPNGVLALNL
                     ASSGEGGDLQRASAVVQTLKTAFPTLESFGVKGPWRAHQTTAENLIFFGGGPIDTMPY
                     DEVVKRIQSHVEARRLPPEATALLAARRTQPWSPGLTLTDDYTPYDLLIGSHAVEMSP
                     EGKALP"
     gene            3475..3954
                     /locus_tag="PK614_05775"
     CDS             3475..3954
                     /locus_tag="PK614_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81754.1"
                     /translation="MGKTMVAVFFGLVMLVAGASGAYALQAGGVEIGDLDTGSVVGQP
                     FKELVVDAELYAVEIGNMKAWYPPVTVIDFKVRPGRPVVLKVTNNSTAEHGFQMTDAA
                     NSGSPFVMKAEVVLKPGETKYIGVPTSDMFYATQGNTLTYRCHLHPAHVGGKILMIK"
     gene            complement(4061..4390)
                     /locus_tag="PK614_05780"
     CDS             complement(4061..4390)
                     /locus_tag="PK614_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81755.1"
                     /translation="MTRVSRLILFASLALLPVQTMADTGDIGTIIESFVTKQFPDAAN
                     HFWIVNDTQWDGDEMIVDMNAIVTERRQDAPTASRFLLLIVDGRLRGIQNIPLDAEAE
                     CQKEQEA"
     gene            complement(4595..4942)
                     /locus_tag="PK614_05785"
     CDS             complement(4595..4942)
                     /locus_tag="PK614_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRB81756.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAEGKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCIADPAYPETTEVLLDKAKTLNPDK
                     EIDPAKVWSGVRN"
     gene            5190..5687
                     /locus_tag="PK614_05790"
     CDS             5190..5687
                     /locus_tag="PK614_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRB81757.1"
                     /translation="MVRRASAAESEQRRKKIVTFVLLVILLVSVSLFLGGPKESELII
                     VEFPNGKTMETEVASTPEKLLFGLAFREGLPADTGMLYIFESTGLHRVQTRQFRIAVD
                     MVWIDESHHVVHILEQVPPCEKDPCPLYGPPPEPVRYLIEAEAGSVQRAGITTGMELK
                     FTLRM"
     gene            5704..6021
                     /locus_tag="PK614_05795"
     CDS             5704..6021
                     /locus_tag="PK614_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRB81758.1"
                     /translation="MEGFQCVAKVEEIPPGQVKVVKVNERGIAVFNIDGQFHAIYNSC
                     PHEGGPLNKGRVKGHVVSCPWHDLAFDVRNGQGTDGGGYCVGSYDVRVEDGQVFVGGR
                     RKV"
     gene            6060..6392
                     /locus_tag="PK614_05800"
     CDS             6060..6392
                     /locus_tag="PK614_05800"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRB81759.1"
                     /translation="MSPLSKAVPTGAEQEPGAKTLKARVGASVHIRLWEDRTRGELWV
                     PSYDATVMPLVEDDFLRTASNNAVDAGQRTFEFRPMAAGTHRLVFEKRMGWKFTAEDR
                     QIFYVTVS"
BASE COUNT         1226 a         1664 c         2008 g         1553 t
ORIGIN      
        1 caggcccaac acgccgagcg tttttccctt cacgccgccg caggcctctt tcaccttctc
       61 gaccattcgc agatgttgtt cgtagttcac cttggccgcg gcccctgcga tctggaacgg
      121 gtaccccacg cgctcgccgg tctggaccag tgcagccaag tccttcggga aacacgaacc
      181 tccgaatcct ggcccggcat gcaggaattt gttcccgatg cgattgtcga ggcccattcc
      241 tttggagacc atttgcacgt cggcgcccac cttttcgcag acggtggcga tttcgttgat
      301 gaaggaaatc ttgacggcga gaaacgcatt ggaggcgtac ttgatcatct cagcagttgg
      361 gatgtcggtt acgacaatcg gggtttccag caggtacagc ggacgataga ggtctttcat
      421 gatcgcgacc gcttgctcgc tgtcagcgcc gatcacgacg cggttgggac gcataaagtc
      481 ttcaatggcg gatccttcgc ggaggaattc cggattggac acgatatcga accggaaccg
      541 gccggtttga ttggccttaa ttacctcgcg caacttttct ccggtgccga cagggacggt
      601 ggacttcgtg acgatgacct tgtagccggt catgtttttg gcgatgccgc ggcccacttc
      661 ttcgacatag gacagatcgg ctgagccgtc cgatttcggg ggcgtgccga cggcaatgaa
      721 gatgaccagc gctttgtcga cggccttggc gacatcggtg gtgaaatgaa ggcggtcttc
      781 tttgatgcct ttggcgacga gttcggtgat accgggttcg aaaaacggga cttcaccctt
      841 ctcgagtctg gcgatgcggc gcgcatccgt gtccatacag gtgacgttca cgccgaattc
      901 cgcgaagcag gcaccggtga ctaagccgac atagcccgtt ccaatcacgc tgatatgcat
      961 ggaagcatct cctcctgaag ataagccgtc gaaactgtgg gtaagtacgt gtgtagagtc
     1021 ggtagtatag caacgcgtac gaacgtgagc aaacataacg caggttttgg caggggtgcg
     1081 gacggggcga attgcagatt gccgcgcaca tccagtagaa ttcggccctc ttcgaaggag
     1141 aggctggcga atggcaggtt caggcgggcc catccaacga ccattagcga tgatgatgcg
     1201 tcctatcacg aggaccgtcc atcccgatga caccttgttg gccgtggcca ggcaactgcg
     1261 cgacgcccgc gtcggggcga tgctggtcgc tgatcacggt gactatgtgg ggattgtcag
     1321 cgaggccgat ctcgtccgaa aagccatggc cagcggcgca gacgcggaac aggtcatggc
     1381 ccggtcagtg atgagcaccc cggtcatgac gatcgatatc gcccaatccg cgcatgaggc
     1441 gagcgatgtg atggccgagc ggggaatccg gcatctcgtg attacagaag aggggcgtgt
     1501 ggtggggatg atttcggtgc gcgatctgct tcgctacttc aagaattggg gaacgctcta
     1561 atatggggtg cctcgattcc gaacggcact gttttgcttc tagcctttct gtggtcctgt
     1621 gttgatgatg gcacagggac cttccgcctc tcgcatgcca cgtctgtttc tgctcatcac
     1681 ggcgctcgta accggtgcca tcgttatggc gctggaaatt ctaggaagcc gtttgctggc
     1741 tccggtgttc gggaattcgc tgtttgtctg gggggccttg atcgggatca tcctggcggc
     1801 catgagttca gggtatgcgt ttggcggctg ggcgtcggat cggtatcgtg tcgcggcagt
     1861 gctggcctgg ctcctgctcg gttccggcgc ctggacgttg ctgatgtcct ggacggggca
     1921 ggttgccatt tttaaggttg ccaagcttgt cgaggacccg cggtggggcc ccagccttgc
     1981 ggcctgcgtg ttattggccc ccccggcatt cggattgagc ggcgtcatgc cggcattgct
     2041 tcggttggcc gtggtcgata tggggtatct cggccgccat accggcagca tgattgcgct
     2101 gtccaccgtc gggagtctgg ctgggacctg gggtacggcg ttcttccttc tctcctggct
     2161 ggggacccag acgctcgtcg cttcattggg tgtgctccag cttttgttgg gtttgttgtg
     2221 gttgcagcag gggagcggaa aggtcacgaa agtgacgggt ttgttgctgg ccggtctgct
     2281 tattttgagt tggcaattgt tcgggcaggc ggctcccgtt gccgggctgg tgtaccagga
     2341 ggatagccct tatcagcagg tgcgcgttcg tgacgatgat ctgtttcgct atctggtcct
     2401 cgaccgcacc tggcatgcgg tcatgtggcg gtccgaccct gccacgctct ttctccccta
     2461 tagccaactc atggttgcag cggtcgcgtt gaccccggac cccaaacgcg gactcatttt
     2521 ggggcacggt ggtggatcac tcgccaagtg gctggcccag gtatggccgg agttggagct
     2581 ggatgtcgtc gagtttgacc cggtcgtcgt tcggatggcc caggagtatt ttgagtatca
     2641 cccccccgcg aaccatcatg tcttcgtcaa agatgcccgt gtgtttgtgc gcgatacaga
     2701 ggtgaagtac gacgtcatct gggtcgatgc gtttgcccgg cacctcatcc cctttcattt
     2761 gaccacggtt gagtttttct cagaactccg gagtcgactg aatcccaatg gcgtgctggc
     2821 cctcaatctg gcgtcatccg gcgagggtgg agatctccag cgtgcgagcg cggtcgtgca
     2881 gacgttgaag acggcgtttc ccacactcga gtcctttggc gtgaaagggc cttggcgggc
     2941 gcatcaaacc acggcggaga atttgatttt tttcggcggc ggtcccatcg atactatgcc
     3001 gtacgacgag gttgtgaagc gtattcaatc gcatgtcgaa gctcggcgac tgccacccga
     3061 agcgactgcc ttgttggcgg cgcgacgcac ccagccctgg tcgcctggcc tgaccttgac
     3121 cgacgactat acgccctacg atttgctgat cggatcgcat gcggtagaaa tgtcgccgga
     3181 gggaaaggct ttgccgtaaa catatgaaat tatgaatgga atgaagcctg tcgagtcttt
     3241 ggtccgtaag acatttcgtg gggtaactag gtccgcttca cgtccaagaa ctttcttgct
     3301 tcctttttaa tccctatgct ataaggtgtc cctcatcagc ctattattta ggacattttc
     3361 gagtagtaga ggctaggctc ctgggcggag tgtctagcag atggttcaga gcgcggtggt
     3421 cattattcac caacctaatc gcatctcttc ttaatctctt taaggaggta ggtcatgggt
     3481 aagacgatgg tagcagtgtt ttttggcctg gtcatgctgg tggctggggc atcaggcgca
     3541 tatgcgcttc aagctggtgg tgtcgaaatc ggtgatttgg ataccggttc tgtcgtcggt
     3601 cagccgttta aggaattggt cgtggatgcg gaattgtacg ccgtcgaaat cggcaacatg
     3661 aaggcgtggt atccgcctgt caccgtgatc gactttaaag ttcgtccggg ccgtccggtc
     3721 gttttgaaag tcacgaacaa ctcgaccgca gagcatgggt tccagatgac cgatgctgcg
     3781 aattccggtt ccccgttcgt gatgaaggct gaagtcgtgt tgaagccggg agaaaccaag
     3841 tacatcggtg ttcccaccag tgacatgttt tatgccaccc agggtaacac gttgacctat
     3901 cgctgccatt tgcatccggc ccacgtcggt ggcaagatct tgatgatcaa gtagtcgacg
     3961 tttcaaatac gtgtagtgca acgccccatc tgcagtgatg cagatggggc gttgttgtat
     4021 caggcgcatc gccggctggt atcgatacaa tgtttccttc ctaggcttcc tgctcctttt
     4081 ggcattcggc ttcggcgtcc agaggaatat tctgaatacc tctgagtctc ccgtctacga
     4141 tgagtagcaa aaagcggctt gctgtggggg catcctgccg ccgctcggtg acaatggcgt
     4201 tcatgtccac gatcatttcg tcgccgtccc attgcgtgtc attgacgatc cagaagtgat
     4261 tggctgcatc cggaaactgt ttcgtgacga agctttcgat gatggttccg atgtctccgg
     4321 tatcggccat ggtttggacg ggaagcaggg cgaggctggc gaataagatg aggcgactta
     4381 cgcgtgtcat gagcacctcc cgctgagagg gtgacgaatg ggcggagtgc agcaaggcct
     4441 taagggtggg ggcctgcgca ggccgcctcg ctcttaaagt ctacagctgt tggccagtga
     4501 aagccaggga gttatctctt ttgcagtgca gaaacgccga aggctcccgg aggagccttc
     4561 gtggtgtcac ctataggaga atgacgtatt ctgactagtt tcgaaccccg ctccacacct
     4621 tggccggatc aatttcttta tccggattca aggtcttggc cttgtcgagc aacacctcgg
     4681 tggtttcggg gtaggccgga tcggcaatgc agcaattgtc taccgggcag accgccgcgc
     4741 actgaggttc gtcaaagtgc ccgacgcatt ccgtgcaacg atcatgagtg atgacataaa
     4801 tgttgtcacc gactccctga ccgtcgccga cgtggttgcc cttgccttca gcgtcgctgc
     4861 gggtttcaaa gatcgcttcg ttgggacatt ctggcaggca tgccccgcag gaaatgcact
     4921 catcggtgat cagaagcgcc atggcctgtg cctccttctc agtttcttta atgacggata
     4981 agatgttgac aatttcgtgc cgccgcgacc atagttcgcg gcgaaacagg tccgcattct
     5041 atgccgccgt tcttcggcaa gtcaacagag tgagactcgg gaagaaggcc tagcactcgg
     5101 aaagtcatgg ggctttgggc ccaatgtaca tgcggctctg cgcgtgtgcg atcacatgag
     5161 gatgcatcca tgttcgaccg catttggtta tggtgaggcg cgcgagtgcg gctgagtcgg
     5221 agcagcgtcg caagaaaatt gtgacgttcg tgctcttggt tattttgttg gtcagcgtga
     5281 gcctgtttct tggggggccg aaggagtccg agctcatcat cgtggaattt cccaacggaa
     5341 agaccatgga aacggaagtg gccagcaccc ctgagaaatt gttgttcggt ttggcgtttc
     5401 gagaaggatt gccagccgat acggggatgc tttatatctt tgagtcgacg ggtttgcatc
     5461 gcgttcagac tcggcagttt cgcatcgccg tcgacatggt gtggattgat gagagccacc
     5521 acgtggtcca catcctggaa caggtgccgc cctgcgagaa ggatccgtgt ccactctatg
     5581 ggcctccgcc cgaaccggtt cgctacctca tcgaggctga agcgggctct gtccagcgcg
     5641 caggcatcac gacggggatg gaactcaagt ttacgcttcg tatgtaaaga atcggagtcg
     5701 tttatggaag ggtttcagtg cgtggccaag gtcgaagaga ttcctcctgg gcaggtcaag
     5761 gttgtgaagg tcaacgagcg gggcattgcc gtatttaata tcgacggtca atttcacgcg
     5821 atctacaatt cctgccctca cgagggcggg cctttaaata agggccgggt gaaggggcat
     5881 gttgtctcct gtccctggca tgatctggcg ttcgatgtcc gaaatgggca aggcacggat
     5941 ggcggcggct actgtgtcgg gagctatgat gtgcgggtgg aagacgggca ggtgtttgtc
     6001 ggggggcggc gcaaggtata gtcgggcgag tgattgcctc ggggcagcat gagaatagta
     6061 tgagtccatt aagtaaggca gttcccaccg gtgcagagca ggagccgggc gccaagacgc
     6121 tcaaggcgag agtgggggca tcggttcaca ttcggctgtg ggaggatcgc acccgcgggg
     6181 agttgtgggt tccgtcctac gacgcgaccg tcatgcctct ggttgaggac gattttcttc
     6241 gcactgccag taacaatgcg gtcgacgcgg ggcagcgtac gtttgaattc aggcctatgg
     6301 ccgcaggaac ccaccggttg gtgtttgaaa agcggatggg gtggaagttc acggctgagg
     6361 accgacagat tttttacgtg accgtatcct gaggttcgaa aggtgctgag aaatgagtca
     6421 tgcctgacat tgcgtatgtg aatggtcgct t
//
LOCUS       DAORQN010000076         6435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_542_length_6435_cov_13.053292, whole genome shotgun sequence.
ACCESSION   DAORQN010000076 DAORQN010000000
VERSION     DAORQN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_542_length_6435_cov_13.053292"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PK614_05805"
     CDS             <1..1193
                     /locus_tag="PK614_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB81760.1"
                     /translation="HWPTLAGASLHLTVSFMVWLLFGALAVAIGQELGLSATQQSVLV
                     ALPLLGGAVLRIAAGWACDWYGAKRTGLLVLGVQLLAVIWAVFGGRSYAELLGIALLL
                     GAGGASFAVAMPVASRAYPATHQGLVLGLVASGNIGTVLVLLLAPRWETALGWHWACG
                     IMAVPILIAIGLFTTVVQSEPTVRTAGEGAWWQAAWAMARVPSVYWLCMIYGVTFGGF
                     VGLTSFLPVLLHDQYGSDPIVGGSIAALCGFTGSLIRPVGGHVADRWGGLPVLVGVFV
                     ALASMTVLAGSLPALPWAVGLLVVTIAVMGFGNGVIFQIVSEWFPTDIGLASGLVGAA
                     GGIGGFLVPIGFGLLRESTGTFLFGFVGLAVVSAAAAMTVAPALRWRPQAVAQAPSPG
                     GFSR"
     gene            1259..2518
                     /gene="ribB"
                     /locus_tag="PK614_05810"
     CDS             1259..2518
                     /gene="ribB"
                     /locus_tag="PK614_05810"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRB81761.1"
                     /translation="MATPFNSIEDAIKDIKKGKFIILVDDEDRENEGDLVMAAGKVTP
                     HAVNFMAKHARGLICLALTPERVEELQLAPQAAENTATFGTAFTVSIDARKGITTGIS
                     AADRATTIHVAIDPRSTPADLARPGHIFPLKSQVGGVLRRAGQTEGSVDLARLAGLYP
                     AGVICEIMNEDGTMARVPELTKFARRHKLKMVTIKALIEYRMHRETFVRRAASALLPT
                     TFGDFEAIAFENDIDGATHIALVKGRVDAETPMLVRVHSGCLTADVLGREQLHRAMEI
                     IQKDGRGVLLYLNQEGRGIGLLNKIRAYGLQDSGSDTVEANLQLGFKADLRDYGVGAQ
                     ILVQLGLHRIRLITNNPRKIVGIEGYGLKVVERVPIEIAPPCRQRKISPHQKSQARPP
                     LKKGLASLQPVSILSHPRVGEMRAPTA"
     gene            2612..3154
                     /gene="ribH"
                     /locus_tag="PK614_05815"
     CDS             2612..3154
                     /gene="ribH"
                     /locus_tag="PK614_05815"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRB81762.1"
                     /translation="MKLRKGSQDATGLTFGIVASKFNKFVTSRLLTECVKALTKAGVT
                     DEAIEVVRVPGAFEIPLVARQLARSGRFDAVICLGAVIRGDTPHFEYISTEVSRGILQ
                     ASMDSNIPVIFGVLTTDTVAQAIERADPEKLNRGADAAKTAIEMVNVMRLLKKEGSGE
                     APQPAAPSRRAGRSAARPKR"
     gene            3168..3620
                     /gene="nusB"
                     /locus_tag="PK614_05820"
     CDS             3168..3620
                     /gene="nusB"
                     /locus_tag="PK614_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRB81763.1"
                     /translation="MASRHQARERAVQILFQYDVHGQAGPWLDDFWIQYPLAEELRVF
                     AERLVAGVRANKKELDVLIGTYATNWKVSRMPIIDRNILRMGCFELLHVPEVPAKVTL
                     NEAIELAKSFGDEEASKFVNGILDKVLGSDARLERKRAFPELPVVGSE"
     gene            3751..5085
                     /gene="purB"
                     /locus_tag="PK614_05825"
     CDS             3751..5085
                     /gene="purB"
                     /locus_tag="PK614_05825"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRB81764.1"
                     /translation="MIDRYTRPRMSAIWDLQHKYEIWLEVELQACAAFERGGQIPRGT
                     SARIRKKAKIDVARIAEIEKVTKHDVIAFLESLMDSVGPEHRFLHMGLTSSDIVDTSL
                     AVQMTEALDLILEDLDELIAVLKKRAIEHRHTVMVGRSHGIHGEPVSFGFKLAIWYEE
                     ACRHRTRLEAARKDIAVGKLSGAMGTFAHQGPEIEEYVCAKMGLASDPVSNQIVQRDR
                     HAAYASALALLAATIEKIATEIRHLQRTEVLEAEEYFSAGQKGSSAMPHKRNPIASEN
                     LCGLARLVRGNSLAALENVALWHERDISHSSVERVIMPDSTILVDYMLARVTDLVKNL
                     IVYPERMQRNLDLTGGLVYSQRLLLALIDKGAQRKESYEAVQRNAMTAWKGGVGFQEL
                     VARDPFITTHLKPHEITACFDPAYYLRHLDQVFRRVFGASGASSARRRRKRA"
     gene            5082..5876
                     /locus_tag="PK614_05830"
     CDS             5082..5876
                     /locus_tag="PK614_05830"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRB81765.1"
                     /translation="MSLRSTARVGLLAGLLVLAAVWPVSAADREKLLTEPGVYGTFAT
                     FQMDHDWWDLPGESRVISVAEVKGLVEQWSGKILVESYLLRGLSDHADLMFRVHARTL
                     SDTQQFLSAFMGTRLGRHLTSGGLLHGVSKKPTYVAGFPESMKTELQVNGESGSRPYA
                     IVIPIKKDAEWWGLDQEARTALMQEHTQAALPYLKTVKRKLYHSTGLDDVDFITYFET
                     EKLEDFHNLVRALQQVKEFRHNRRFGHPTLLGTMSPLDEILEKFAQ"
     gene            5892..>6435
                     /locus_tag="PK614_05835"
     CDS             5892..>6435
                     /locus_tag="PK614_05835"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRB81766.1"
                     /translation="MTVEPAGTMIYEGKAKKIFTTDRSDQVLQYFKDDATAFNAQKRG
                     TIVDKGVVNNKVSERLFRLLEQQGISTHFIERVSDREMLTKRVTIVPVEVVVRNVVAG
                     SLAKRLGLKEGAAIDPAIIEFYYKDDALGDPLVNDDHLRLLNVATPAVLREMRELGHK
                     INAVLLPFFKERRMMLVDFKL"
BASE COUNT         1241 a         1730 c         2122 g         1342 t
ORIGIN      
        1 gacattggcc gaccctggcc ggcgcctcgc tgcatctcac cgtgagtttc atggtgtggc
       61 tgttgttcgg ggccttggcc gtggcgatcg ggcaggaact cgggttgagc gcgacccaac
      121 agagtgtgct ggtggcgctg ccgttgctgg gcggcgcggt gctgcgaatt gccgccggct
      181 gggcctgtga ttggtatggg gcgaagcgga ccggtctgct ggtgctgggc gttcaactgc
      241 tcgcggtcat ctgggcggtg tttgggggga gaagttatgc ggagctcctc ggcattgcgc
      301 tcctgcttgg tgccggaggg gcgagttttg cggtagcgat gcccgtggcc agccgggcct
      361 atcccgcgac ccatcaaggt ctggtgttgg gcctggtggc ctccggaaat atcgggaccg
      421 tgctggtgct gctgctcgct ccgcgatggg aaacggcact cggatggcac tgggcgtgcg
      481 gaatcatggc cgtccccatt ctgatcgcga tcggtctctt taccactgtg gtgcagagcg
      541 agccgactgt gcgcacggca ggagaggggg cctggtggca ggcggcctgg gcgatggcgc
      601 gggtgccgtc ggtttattgg ctttgtatga tttacggcgt cacgttcggc ggattcgtcg
      661 ggctgacgag ttttctcccg gtgttgttgc acgatcagta tgggagcgat ccgatcgtcg
      721 gtgggtctat cgccgcgctc tgcgggttca cgggaagtct gattcgtccg gtcggcggcc
      781 atgtggcgga tcgatggggc gggctgccgg tgctggtcgg ggtgtttgtt gcactcgctt
      841 cgatgacggt gctggcgggg tcacttccgg cgttaccctg ggccgtagga ctgctggtgg
      901 tgacgattgc ggtgatgggg ttcgggaacg gggtgatttt ccaaatcgtc tcagagtggt
      961 ttccaacgga catcggtctg gcatccggcc tggtgggcgc tgccggcggg atcggcgggt
     1021 tcctggttcc catcgggttc gggttgctgc gggaaagtac gggcacgttc ctgttcgggt
     1081 ttgtgggatt ggcagtcgtg agtgccgccg cggcgatgac ggtggctccg gccctgcggt
     1141 ggcgtccaca ggccgtcgcg caggctccga gccccggtgg tttttcccgt tgacaggggt
     1201 ggtttgaaaa gagttagctg ggccaccgaa gcaggccggc ctgcaccatc gagggattat
     1261 ggcgacaccg tttaattcga tcgaagacgc gattaaggat atcaagaagg ggaagttcat
     1321 catcctcgtc gatgacgaag atcgtgaaaa cgagggcgat ctggtgatgg cggccggcaa
     1381 ggtcacgccg cacgccgtca acttcatggc caaacatgcg cgtggcctca tctgtctggc
     1441 gctgactccg gagcgggtgg aggagttgca gttggcgccg caggcggcag agaacacggc
     1501 caccttcggc accgcgttca cggtctccat tgacgctcgc aaagggatca ccaccggtat
     1561 ctcggcggcc gatcgtgcca cgaccattca tgtcgcgatc gatccccgca gcaccccggc
     1621 cgatcttgcg cgccccggcc acattttccc cctgaagtca caagttggcg gggtgcttcg
     1681 gcgcgcaggg cagacggaag ggtcggttga cctggcgcga ttggccggac tctatccggc
     1741 cggcgtcatc tgcgagatca tgaatgaaga cggcacgatg gctcgtgtgc cggaattgac
     1801 caagtttgcc aggcggcaca agttgaaaat ggtcacgatc aaggcgttga tcgagtaccg
     1861 catgcatcgt gagacgttcg tgcggcgagc cgccagtgcg ttgctgccca ccaccttcgg
     1921 cgattttgaa gcgattgcgt tcgagaacga tatcgacggc gcgacccaca ttgcgctggt
     1981 gaaggggcgg gtggatgccg agacgccgat gctggtccgg gtccattccg gttgtctgac
     2041 ggcggatgtg ctgggtcgtg agcaactcca tcgggcgatg gagatcattc agaaggacgg
     2101 tcggggcgtg ttgctctatc tcaatcaaga gggacgcggg atcggcctgc tgaataagat
     2161 tcgtgcctac ggtctacagg attctggcag cgataccgtc gaagcgaatc tgcagctcgg
     2221 tttcaaggcc gatctgcgcg actatggcgt cggcgcacag atcctcgtgc agctcgggct
     2281 gcaccgcatc cggttgatta ccaacaatcc gcgcaaaatt gtcggcattg aaggctacgg
     2341 cttgaaggtg gtcgaacggg tgccgatcga gatcgccccc ccgtgccgac aacgaaaaat
     2401 atctccgcac caaaaaagcc aagctcggcc acctcttaaa aaaggtctag cgtcgctcca
     2461 gccggtctca atcctgagtc atcctcgtgt gggagaaatg cgtgcgccga cggcctgaaa
     2521 cgtatagcca tttgagggcg tttccaggta tgattcccct ctttcttcgg cgcacacagt
     2581 ccatgagacc tctggctcgt tatctcattt catgaagctt cgcaaaggtt cccaagacgc
     2641 gacggggctg accttcggca tcgtggcgag caaatttaat aagtttgtca ccagtcggtt
     2701 attgacggag tgcgtgaagg cgctgacgaa ggccggcgtg acggacgagg ccatcgaggt
     2761 ggtgcgggta ccgggcgcgt ttgagattcc cctcgtggct cgccagcttg cccggtccgg
     2821 tcgatttgat gcggtgatct gtctcggcgc agtcattcgt ggtgatacgc cgcattttga
     2881 gtatatcagc accgaagtca gtcgagggat tctgcaagcc tcgatggact cgaatatccc
     2941 ggtcatcttc ggcgtcctca ccaccgatac cgtggcgcaa gcgattgagc gcgccgaccc
     3001 cgaaaagttg aatcgcggcg ccgatgcggc gaaaacggcg atcgaaatgg tcaatgtgat
     3061 gcgattgctc aaaaaggaag gaagcgggga ggcgccgcag ccggctgccc cgtcacgacg
     3121 agccgggcga tcggcagcga ggccgaaacg ctgaccggcg ttgcactatg gcctctcgac
     3181 atcaagcccg tgaacgggcg gtacagattc tctttcaata cgacgttcat ggccaggcgg
     3241 gtccctggct ggacgatttc tggattcagt atcccttggc cgaggagttg cgggtattcg
     3301 cggagcgatt ggtcgccggc gtccgggcca acaaaaagga actggatgtg ctgatcggga
     3361 cctatgcgac caattggaag gtcagccgga tgccgatcat cgaccgcaat attctccgca
     3421 tgggttgttt cgaattgctg catgtgccgg aggtaccggc caaggtgacg ctgaacgaag
     3481 cgatcgaact ggccaagagt ttcggcgacg aggaggcttc caagttcgtg aacggcattc
     3541 tcgacaaggt gctcgggtcg gatgcacggt tggaacggaa gcgcgcgttt cccgaactgc
     3601 cggtggtggg ctccgaataa gagcctatag ctcatggcct atagtagatg gcaagagatc
     3661 caaagttaat cctaattttt gccatacgcc attggccata cgctctgttt ccctggatga
     3721 cgagatgcga gcgacgaatt atgagggaaa atgattgacc ggtatacccg tccgcggatg
     3781 agtgccatct gggaccttca acacaagtat gaaatctggc tggaagtgga gttgcaggcc
     3841 tgcgccgcgt ttgagcgtgg cgggcagatt ccacgcggga ccagcgccag gattcgcaag
     3901 aaagccaaga tcgatgtggc gcgcattgcc gaaatcgaaa aggttacaaa acacgatgtg
     3961 atcgccttcc tcgaatcact gatggattcc gtcgggcctg agcaccggtt cctccacatg
     4021 gggctcacct catcggacat cgtcgatacg tcgctggccg tgcagatgac cgaagcgctc
     4081 gatctgattc tcgaggatct cgacgagctg attgccgtgt tgaagaagcg ggcgatcgag
     4141 catcgccata cggtgatggt aggccggtcg cacggcattc atggcgagcc ggtgtcgttc
     4201 gggttcaagt tggcgatttg gtacgaggag gcctgccggc accggacgcg cctcgaagcg
     4261 gctcgcaagg atatcgccgt aggcaagctc tcgggagcga tgggcacctt cgcgcatcaa
     4321 ggccctgaaa ttgaagaata tgtctgcgcc aagatggggc tcgcgtcgga tccggtgtcc
     4381 aaccagatcg tgcagcgaga ccggcatgcg gcctatgcct ccgcgcttgc gctactggcc
     4441 gcgaccattg aaaaaattgc caccgaaatt cgtcacctgc agcgcacgga ggtgctggag
     4501 gcggaagaat atttctccgc cggacagaag ggctcatcgg cgatgcccca caagcgcaat
     4561 ccgatcgcgt cggaaaatct gtgcggcctg gcgcgcctgg ttcgtggcaa tagcctggcc
     4621 gcgctggaga acgtggccct gtggcatgag cgtgatatca gccactcctc ggtggagcgg
     4681 gtgatcatgc cggacagcac catcctggtc gattacatgt tggcccgcgt gaccgacctg
     4741 gtgaagaatc tgattgtgta tcccgagcgg atgcagcgaa acctcgacct gaccggtggc
     4801 ttggtctatt cgcagcgact gctgctggcc ttgatcgata agggcgccca gcgcaaggag
     4861 tcgtatgaag cggtccagcg caacgcgatg acggcttgga aaggcggagt cgggtttcag
     4921 gaactggtcg ctcgcgatcc gttcatcacg acccacttga agccgcacga aatcaccgcc
     4981 tgtttcgatc cggcctatta ccttcgacat ttggatcaag tctttcgccg ggtgttcgga
     5041 gcgagcggcg cttcgtccgc cagacgacgg aggaaacggg catgagcctg cggtcgacag
     5101 cacgagtcgg gctcttggcc ggtttgctgg tgctggccgc ggtttggccg gtttccgcag
     5161 ccgatcgcga gaagcttctg acagagccgg gggtgtacgg cacatttgcg accttccaga
     5221 tggaccatga ttggtgggac ctcccgggcg agtcgcgcgt catttcggtg gcggaggtta
     5281 agggattggt cgagcagtgg tccggcaaga ttttggtcga gtcctatctg ctccgggggt
     5341 tatccgacca tgccgatctc atgtttcgcg tgcacgcccg cacgttgtcg gacacacagc
     5401 agtttctctc ggcattcatg ggcactcgat tgggacgcca tctcacatcc ggcggacttc
     5461 ttcatggcgt gagcaagaag ccgacctacg ttgccggctt ccctgaatcc atgaagacag
     5521 agttacaggt gaacggcgaa tccggttcca ggccgtacgc aattgtgatc cctatcaaga
     5581 aggacgcgga atggtgggga ctggaccagg aagcacggac cgccctgatg caagagcata
     5641 cgcaggcggc gcttccctat ctgaagacgg tgaaacgaaa actctatcat tcaacgggtc
     5701 tcgacgacgt cgatttcatc acctattttg aaacagagaa gttggaggac tttcacaatc
     5761 tggtgcgggc gttgcagcag gtgaaggaat tccgccacaa tcgacgcttc ggacatccga
     5821 ccctcctggg cacgatgagt ccgctggatg agatcctgga aaagttcgca cagtagtcga
     5881 aggagagagg gatgaccgtc gaaccagcag gcacgatgat ctatgagggc aaggccaaga
     5941 agattttcac gactgatcgg tccgaccagg tgttgcagta tttcaaggat gacgccacgg
     6001 cattcaatgc gcagaagcgc gggactatcg tcgataaagg ggtggtcaat aacaaggtgt
     6061 cggagcggct gttccggctt ctcgaacagc aggggatttc gacgcacttc atcgagcgcg
     6121 tcagcgaccg tgagatgctc accaagcgag tgacgattgt gccggtcgaa gtcgtggtcc
     6181 ggaacgtcgt tgcggggagt cttgccaagc ggctgggcct caaagaaggc gcggccatcg
     6241 acccggcgat catcgagttt tactataagg acgatgcgtt gggggatccg ctggtgaacg
     6301 acgatcacct gcgcctattg aatgtggcga ctccggctgt gttacgggag atgcgcgagc
     6361 tgggccacaa gatcaatgcc gtgttgctgc cgtttttcaa agaacgccgg atgatgctcg
     6421 tcgatttcaa gttgg
//
LOCUS       DAORQN010000077         6137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_560_length_6137_cov_56.382276, whole genome shotgun sequence.
ACCESSION   DAORQN010000077 DAORQN010000000
VERSION     DAORQN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_6137_cov_56.382276"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /gene="yajC"
                     /locus_tag="PK614_05840"
     CDS             <1..347
                     /gene="yajC"
                     /locus_tag="PK614_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRB81767.1"
                     /translation="TAAQAGDIMWDSVAWAQGTSGSTGAGGGLLSLVPFVLIFVIFYF
                     MLILPQQKRRKQADTMLAALKKGDKVVTASGIWGTITNLGKDTVTLQIADTTKIKIQK
                     EHIARLRGEEED"
     gene            383..2032
                     /gene="secD"
                     /locus_tag="PK614_05845"
     CDS             383..2032
                     /gene="secD"
                     /locus_tag="PK614_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRB81768.1"
                     /translation="MKKVGGRLLALVTMVVASIIFFLPSYQPLYKELPRWVREVLPDK
                     GITLGLDLQGGIHLVLEVEEDRAVEIAVERTAAGLQDLLVEKKIPAESVKRTGSSQIT
                     IGFQNAELKAQVQKLLDDFPTYVEVESAGSANKVVWELRDAEIKRIKDSAINQALETI
                     RNRIDQFGVAEPLIQRQGLKQVVVQLPGIKDAKLAKDLIKQTALLEFKLLDDENQLKL
                     DLPGRIQKGKEREDALLKQVEGKVPEGDQILFERIVEKDSGQEWLAPYLVKKRVMLAG
                     DVLSDARVSIGQFNEPYVSVTFDAKGAREFDRITGENVKKRMAIVLDNTIYSAPVIQE
                     RISGGRAQITGTFSMEEANNLSIVLRAGALPAPLKIIQDLTVGPSLGQDSIEKGVKAT
                     LFAGLLVIVFMALYYRLSGVIANFALLLNLICLIGALAGLNATLTLPGIAGIILTIGM
                     GVDSNVLIFERIREELRQGKAVRVAIDAGYDKALLTIVDSHVTTLITGFALFLFGTGP
                     IKGFAVTLCLGIAINLFTALVGTKVVFDLFNQRRKIEQLSI"
     gene            2248..3165
                     /gene="secF"
                     /locus_tag="PK614_05850"
     CDS             2248..3165
                     /gene="secF"
                     /locus_tag="PK614_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRB81769.1"
                     /translation="MFEILGKTNIDFMGKRSITFAISGILAFLGLIAVLQIARGAANL
                     GIDFAGGTAVQLKFEQAMKIDEARRALETNGLGSAELQEFTQDNKLLIRVKASTTIEE
                     KVAERVMAVFSKEFPANKFVVDSSMEIGPTIGKKLQEDAMIAVLISFVGIVLYIAVRF
                     ELRFGVAAALATFHDVLAVLGVFYLLDKEITLLVITALLTLAGYSLTDTVVVFDRIRE
                     NLRTRRREDEETIINQAINQVLSRTIVTSLTVVIVLIPLVLAGAEVLHDFSLALLGGV
                     MFGTYSSVFVASPLLLLWPGSAGGLMKRR"
     gene            3241..>6137
                     /locus_tag="PK614_05855"
     CDS             3241..>6137
                     /locus_tag="PK614_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB81770.1"
                     /translation="MLFWSRSHSLQRKIITAIVMVGLLPLCLSLVLTYIEERRALRET
                     IGANFKEVAVEAARRIEMQVTRGVNEAQQLAATPFLRTSVTESNRTYLDKDEKTVQSM
                     IKAWQQRWRQRDKQSEFPLFINRIVTNYLIRWHDIRKADYVGIFVTDNRGALVVSSIP
                     QVEYYHGKAAWWQAVMKRGTGKVFVSDIFFDPAFGTHVVNVSAPIIDDEQHVTIGAVT
                     VLLRRDTLFHSVSEATVGHTGHAMLFNSDGVPLICPVLAPEEHAVKPELVSAISASPA
                     GWTTLANDSHGGQNSIVGFAPVRLGTELTPDSLGGKRWVTFVRQDPAESYAPLSRLVV
                     QVTVYGLGVFGVLLLTGLTVARRIARPIGLLHEGVQRIGSGRLDQQLDLKTGDEIEQL
                     ADAFNKMAANLRISFEQIEQRMEDVRRLEARYRDLIEHSPEMIYQLNKAGQFVHVNKT
                     GLDKLGYSLEEMLQMRLWDLVPKGREAEVLAYLERLVSQGRSTIETVFVAGDGHPIDV
                     EIHATALFESGGGLVHSRAFVRDVTERRLLEAQIHQYTTRLEQAVNERTRQLVASQER
                     YKALFDLVADSVFMVGEDGVIVAVNKREEQALGYSEQQVVGSSILNVVEPVHHDEMRV
                     LLEKIRAGERQVPTQEITVRDATGRETPVEMDLILVRGSDHTWVMVQLRDITDRKRLE
                     RQMHTYQQELEAKVSERTREIEETKQYLENLLENANDVIYTLDSEQCFTYVNSKVLAW
                     GYAKEDLLGRPYLGLLSKRHRGRRLKSTLDIGVKQVYEVEVLTKAGEPRSVMVSVSPL
                     HGVEGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHEINNPLGGILNCL
                     YNMRKGTLSPSRQEEYLASMEDGLRRVQRIVRQLLDFSQQHNPELALADINQVVNRVL
                     LLTDHLFVPHRVQLETALSPDIPELMIDRHMMEQVLMNLVLNAIQAMRTGGV"
BASE COUNT         1296 a         1570 c         2003 g         1268 t
ORIGIN      
        1 taacggcggc acaggcgggg gacattatgt gggattcggt ggcatgggcg cagggaacat
       61 cagggagcac aggcgcagga ggtggtctcc tctctctggt tccgttcgtc ctcattttcg
      121 tcattttcta tttcatgctg attctgccgc agcaaaaacg caggaagcag gcggacacta
      181 tgctggctgc tcttaagaag ggcgacaagg tggtcacggc ctccggaatc tgggggacca
      241 ttaccaattt ggggaaagac acggtgacgc tccagatcgc ggacacgacc aaaatcaaaa
      301 ttcagaaaga gcatatcgct cggctgcgcg gtgaagaaga agactgacgc acgcgacgga
      361 gacgggaagg attggcgcac gtatgaaaaa agtcggcgga cggttgttag ccctagtgac
      421 gatggtggtc gcctcgatca tctttttctt gccctcatac caacctctgt acaaggaact
      481 gccgcggtgg gtgcgtgagg tcctgccgga caagggcatc accctggggt tggatttgca
      541 gggcggcatt cacctggtgt tggaggtgga ggaagatcgg gccgtcgaga ttgccgtcga
      601 gcggacggcg gcggggctcc aggatctgtt agtggaaaag aaaatcccgg cggagtcggt
      661 gaaacgtacc ggatcgtcgc agatcaccat cggatttcaa aatgccgaat tgaaggcgca
      721 ggtccagaaa ctgttggacg atttcccgac ctatgtggag gtggagtcgg ccgggtctgc
      781 aaacaaggtc gtctgggagt tgcgggatgc ggagatcaag cgcatcaagg actccgccat
      841 caatcaggcg ttggagacaa tccggaatcg catcgaccag ttcggtgtgg cagagccgtt
      901 gattcagcga caggggctca agcaggtggt cgtccagttg cccggtatca aagacgccaa
      961 gcttgccaag gacctcatca aacagaccgc gctgctcgag ttcaaattgt tggacgatga
     1021 gaatcagctc aagctggatt tgcccggccg aattcagaag ggcaaggagc gcgaggacgc
     1081 cctcctgaag caggtcgagg ggaaagtgcc ggaaggcgac cagattctgt tcgagcggat
     1141 cgtcgaaaag gacagcgggc aggaatggtt ggcgccctat ctggtgaaaa agcgggtgat
     1201 gctggccggc gacgtgttga gcgatgcgcg ggtctccatc ggccagttca atgaacccta
     1261 cgtgtccgtg acgttcgatg cgaagggcgc gcgggagttc gaccgtatca cgggcgagaa
     1321 cgtgaagaag cgcatggcga tcgtgctcga caataccatc tattcggcac cggtcattca
     1381 ggaacgcatc agcggcgggc gggctcagat caccgggacg ttttcgatgg aagaagcgaa
     1441 caacctgtcg atcgtgttgc gcgccggggc gttgcctgca ccgcttaaga tcatccagga
     1501 cctgaccgtc ggtccgtctc tcgggcagga ttccatcgaa aaaggtgtga aggctaccct
     1561 ctttgcgggc ctccttgtga tcgtgttcat ggccctgtat taccgcctat ccggcgtgat
     1621 cgcaaacttt gcgctcttgc tgaacctgat ctgtctgatc ggggctctcg cggggctgaa
     1681 cgccacgctg accttgccgg gcattgccgg tatcattctg accatcggta tgggtgtgga
     1741 ctcgaacgtg ttgattttcg agcgcatccg cgaagaactc cggcagggga aggccgttcg
     1801 ggtggcgatc gatgcgggat acgacaaggc gctgttgacg attgtggact cgcacgtgac
     1861 gacgttgatc accgggttcg ccctgtttct cttcggtacc gggccgatca aaggctttgc
     1921 cgtgacgctc tgcctcggta tcgcgatcaa cctctttacg gcgctggtcg ggaccaaagt
     1981 ggtgttcgac ctgttcaatc agcgcaggaa aattgagcag ctcagtatct agcggagcgg
     2041 tgacaacccg ctctggcaac gtcgaatagg gaatcgctct gtatcataag cgaagcaaca
     2101 gtactcgcag gatggtcaaa aaggccgtcc agcgaggccg cagtgagtga agaggcgacg
     2161 cgtactctgc gtcgtacgtt gagcctctga acgatgcgag aacaaagctg gcggacgttt
     2221 tcaacatcct gccaggaagg agccggcatg ttcgagattt tgggaaagac gaatatcgat
     2281 tttatgggga agcggtctat cacgtttgcg atctccggca tccttgcgtt tctgggtctc
     2341 atcgcggtgc ttcaaatcgc gcgcggcgcg gcgaatctgg gaatcgactt cgccggcggg
     2401 acggcggtgc agttgaagtt cgagcaagcg atgaagatcg acgaggcgag acgtgctctg
     2461 gaaactaacg gtctggggtc ggcggagttg caggagttca ctcaagacaa caagttgctc
     2521 atccgtgtga aggcgtcgac gacgatcgag gagaaggtcg ccgaacgggt catggcagtg
     2581 ttcagcaaag aattccccgc caacaaattt gtggtggatt ccagcatgga aatcggtccg
     2641 acgatcggga agaaactcca ggaagatgcg atgattgcgg tcctcatttc gttcgtcgga
     2701 atcgtgctct acattgcggt ccggtttgag ttgcgattcg gcgtggcggc cgcgttggcg
     2761 acattccatg atgtgctggc tgtgctgggt gtcttttatc tgctggataa agagatcacg
     2821 ctgctggtga tcacggcgtt gctgaccttg gcagggtatt cgttgacgga taccgtcgtc
     2881 gtcttcgatc gtatccgtga gaatttgcgg acgcgacggc gtgaggatga agagacgatc
     2941 atcaaccagg ccatcaacca ggttctgagc cgcaccatcg tcaccagttt gactgtagtc
     3001 attgtgctca tcccgctggt gctcgcaggc gccgaggtct tgcacgattt ctccctggca
     3061 ttgctcgggg gcgtgatgtt cggaacctat tcatcggtct tcgtggcgag cccgttgttg
     3121 ctgctgtggc ccggcagtgc cggggggctc atgaagcgtc gctaagctcg ggtcgtccga
     3181 gcctgggctg aggcctgtct attatcgtaa gagggccgtt tgtcggggta agccgcaccc
     3241 atgctgttct ggagccggtc tcacagtctc cagcgaaaga tcatcacggc catcgtgatg
     3301 gtcggcctcc tgccgttgtg cctctcactc gtcctcacct acatcgaaga acgccgggcg
     3361 cttcgggaaa cgatcggggc caactttaaa gaggtggcgg tcgaggctgc ccggcgaatt
     3421 gagatgcagg tgacgcgcgg cgtcaacgag gcgcagcaac tcgccgccac acccttcctg
     3481 cgcacctcgg tgaccgaatc caatcgcacc tacctggaca aggacgaaaa aaccgtccag
     3541 tccatgatca aagcctggca acagcggtgg cgtcaacggg acaagcagag cgagtttccg
     3601 ctgttcatca accggattgt gaccaactat ttgattcgat ggcacgatat ccgcaaggcc
     3661 gactacgtgg gaatttttgt gacggacaac cgcggcgccc tggtggtgag ttcgatcccg
     3721 caggtcgagt attaccatgg caaggcggcg tggtggcagg cggtcatgaa gcgcggaacg
     3781 ggcaaggtgt ttgtcagcga tatcttcttc gaccctgcct tcggcaccca cgtggttaat
     3841 gtgtcggccc cgatcatcga cgatgaacaa catgtgacga tcggtgcggt cacggtcctg
     3901 ttacggcggg ataccctgtt tcattccgtg tcggaagcca cggtggggca cactgggcat
     3961 gccatgttgt tcaattcgga cggtgttcca ttgatctgtc cggttctggc gccggaagaa
     4021 catgcggtga agcctgagct ggtgagcgcc atcagcgcga gtcctgcggg atggaccacg
     4081 ctggccaatg attcgcacgg gggacagaat tcgatcgtgg ggtttgcgcc ggttcgactg
     4141 gggactgaat tgacgccgga tagcctgggt ggcaagcgct gggtgacatt tgtacggcag
     4201 gacccggccg agtcctatgc gccgctctcg cggctcgtcg tgcaagtgac ggtgtatgga
     4261 ctgggggtgt tcggcgtcct attactcacg ggcctgaccg tcgcgcgacg catcgcccgt
     4321 ccgatcggac tgttgcatga aggagtgcag cggatcggaa gcggacggtt ggatcagcag
     4381 ctggatctca agaccggcga tgaaatcgaa caactggccg acgccttcaa taagatggcg
     4441 gcgaacctgc gcatctcgtt cgaacagatc gagcagcgga tggaagatgt ccgccggctg
     4501 gaggcgcggt atcgcgatct catcgagcat tccccggaga tgatctatca gttgaacaag
     4561 gccggccagt ttgtgcacgt gaataagacc ggcctggaca aactcggtta ttccctggag
     4621 gagatgctcc agatgcgcct ctgggatctc gttcccaaag gccgtgaggc ggaggtgttg
     4681 gcctatctgg agcggttggt ctcgcagggg cgcagtacga tagagaccgt gtttgtggcc
     4741 ggggacggcc acccgatcga cgtggaaatt catgcgacgg ccctattcga aagcggtggc
     4801 ggtttggttc actcgcgcgc gtttgtccgc gatgtcacgg agcggcggct gttggaggcg
     4861 caaatccatc agtacacgac cagactggaa caggcggtca acgagcgcac acggcaactg
     4921 gtggcctcgc aagaacggta taaggcattg ttcgacctgg tggcggattc ggtgtttatg
     4981 gtgggggagg acggtgtgat tgtggccgtc aacaaacggg aagagcaggc gctgggctac
     5041 tcggagcaac aggtcgtcgg aagcagtatt ctcaacgtgg tcgaaccggt tcaccatgac
     5101 gagatgcggg tgttgttgga aaagatccgc gcgggcgagc gccaggtgcc gacccaggaa
     5161 atcaccgtgc gcgatgccac cggcagggaa acgccggtgg agatggacct gattctggtg
     5221 cgcggcagcg accatacctg ggtcatggtc cagttgcgcg acatcaccga ccggaaacgt
     5281 cttgagcggc agatgcatac ctaccagcag gagctggaag cgaaggtcag cgagcgaacc
     5341 cgcgagattg aagagaccaa gcaatacctt gagaacctcc tggaaaatgc caatgacgtc
     5401 atttacacgc tcgatagcga gcagtgtttc acgtatgtga acagcaaggt gctggcttgg
     5461 ggttatgcca aggaagatct gttggggcgg ccgtatctgg ggttactgtc gaaacggcat
     5521 cgggggcgcc ggttgaaatc gacgctggat atcggtgtaa aacaggtcta tgaggtcgaa
     5581 gtgttgacga aggccggcga gccgcgttcc gtgatggtca gcgtctcccc gcttcatggc
     5641 gtggagggag agatcctggg cgtgctggga atcgcccgcg atatgacgga cacgaagaag
     5701 ctggagcaac aaatcagaaa ctccgagaag ctggcgtcgg taggaaaact ggctgcgggt
     5761 gttgcgcacg agatcaacaa cccactcggg ggcattctca attgtctgta caatatgcgg
     5821 aagggaaccc tgtcgccgtc caggcaggag gagtacctgg cgtcgatgga ggatggactg
     5881 cggcgggtgc aacgaatcgt ccggcaactg ctcgatttct cgcagcaaca caatccggaa
     5941 ctcgcactgg ctgatatcaa tcaagttgtc aatcgggtat tgctgttgac ggaccatctg
     6001 tttgtccccc accgtgtgca gctggagacg gcgctgtcgc ccgacatccc ggagttgatg
     6061 atcgatcggc atatgatgga gcaggtgttg atgaatcttg tgttgaacgc cattcaagcg
     6121 atgcggaccg gtggagt
//
LOCUS       DAORQN010000078         6107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_561_length_6107_cov_19.860707, whole genome shotgun sequence.
ACCESSION   DAORQN010000078 DAORQN010000000
VERSION     DAORQN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_561_length_6107_cov_19.860707"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1758
                     /gene="wzy"
                     /locus_tag="PK614_05860"
     CDS             277..1758
                     /gene="wzy"
                     /locus_tag="PK614_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HRB81771.1"
                     /translation="MFPSHRIRTGLFLGQCAWGIGLVALLGLQTFYEVSGETAVYCLS
                     LFLVGLSLWSLWSWKTATGHLLDLYSLFLIAVMLFSGGQSFLEVLGLNTHGLLGGKFP
                     IGAVRQALLLVVASMLAFHTGALYGARSSITTIDRSPADGALEQALRTVGAMIIILSI
                     GPAMYELYHDFNLVQTLGYTGEFMRAREVTGANAWTLVIAPFLIPGTLLLLAGSRGRR
                     GNIILSWVLIAVPACIYVLLGMRNHGLKGMVALLWLHAMVIGPIKKSVVVLAATIVLV
                     LVPGLAAVRTERLVGMDKIGALVDSYEAMDNPLVLAMREMGGSFITIIYTTEFVPQIR
                     PFEWGATYLSAVGTIFPNFFWDIHPSIAGGLPSYWLMKEVDPYAAKAGQTLGFSVIAE
                     AYLNFSWVGTLIFMSVIGYVLARFVLWVWKQSNPLGLAIEAIFISFVLFVARGESHLI
                     VRSIFWECLIPYLLVTYLYNRQLTRQCHAQLIDAPSSSLTAAS"
     gene            1817..2950
                     /locus_tag="PK614_05865"
     CDS             1817..2950
                     /locus_tag="PK614_05865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81772.1"
                     /translation="MNRGGAETWLMHVLRAIDRDRFHMDFLVHTDTPAAYDEEIVSLG
                     SQVLSCLSPSRPWIYARNFARAVRHGLPYDVIHSHVHHYSGFILRLAHRSHIPIRLAH
                     SHNDTSEVDRCSGWARRQYLHISQEWIRRYATTGVACSAEAGSALFSPSRGTSLAWRT
                     LPLGIDLASFEAGSDRGQLRSSLGIPTEAFVIGHVGRFMAQKNHHFLVDILLETVKRH
                     PLTFVILVGDGPLRSSVERKIETLGLTKVVRFLGLRSDVPELMLSAMDALVLPSLHEG
                     FPVVLLEAQAAGLPCVVSDVVTAEVDVVNQLVSRVALQNSPAVWADAILATRGRKTRH
                     TQRAALDTLWQSPYSIQHSVTELQRLYTVLAERHVPSASRTYS"
     gene            3376..4176
                     /locus_tag="PK614_05870"
     CDS             3376..4176
                     /locus_tag="PK614_05870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB81773.1"
                     /translation="MFSPGAFTHPLRPFFRLWFYRQQARLCLKASAVAYVTKHTLQRR
                     YPARAGALSASYSDVELDNDAFVSSPRASSLDGTPITLLTVATLAQLYKGQDTLISAM
                     ARCVRQGLDLHLVLVGDGHYRSRLENQAADLGLGERIRFLGELPSGAAVRAELDRADL
                     FVLPSRTEGLPRALVEAMARGLPCVGSTAGGIPELLEPCDLVRPNDVQALAEKVREVV
                     TQPHRRAEMALRNLQRARQYHETLLRKTREDFYRYVKDRTAYARPLRP"
     gene            4291..5556
                     /locus_tag="PK614_05875"
     CDS             4291..5556
                     /locus_tag="PK614_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81774.1"
                     /translation="MAPTPIRLIHITTVPQSLFFVSGQAGYMKARGFEVHGLSSPGGH
                     LSAFAASEGVDVEAIEMPRRITPLQDLIAISKICLSFRRLRPHIVHAHTPKGGLLGML
                     GAWVSRVPIRVYHLHGLPYLTATGWRRRLLMGSEYLSCKLAHRVICVSASVRAVAVDA
                     GLCPADKIRVLLQGSCNGVDAAVRFNPSRLGDEVRNVTRRKYDIPCEATVVGFVGRLV
                     RSKGIVELADAWSTLRQEFPRLHLLLVGPAEPGDPVPSEVERRLQNDPRVHLVGEEWD
                     IPPVYAAMDFLILPTYREGFPIVLLEAAAMGLAMVATTVPGCVDAVQDGVTGTLVPPY
                     DSRRLAAAVRGYLKDPELSRRHGEAARERVLRDFSDRAMWKAVHEEYVTLLKKQGLAV
                     PDAATPSTSADSSVNIDVRNHRLTKPSGS"
     gene            5613..>6107
                     /locus_tag="PK614_05880"
     CDS             5613..>6107
                     /locus_tag="PK614_05880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB81775.1"
                     /translation="MRHQDSSRTAVPSLPKRLFDLVVTASAIPLVLPVIAGLALAAWT
                     IHGSPVLFTQPRPGLHGRPFILMKFRTMKKACNSGGRPLSDAERLTRFGRFLRSTSLD
                     ELPELVNVLKGEMSLVGPRPLLMEYLEEYTPDQHRRHEVLPGITGWAQINGRNALTWE
                     QKFAL"
BASE COUNT         1165 a         1716 c         1723 g         1503 t
ORIGIN      
        1 agttagccgg tcggttttta attgagggtg agcggtctcc atgtctaccg cctcatcgca
       61 acagctttgt gcgtagcgca atcgcgcaat gtgacgcgcc ataaagtcaa aagtcaggac
      121 tcttcataat tttatctgag cccaaatagt atctatcttc tgggcgtggg cctcagtcga
      181 gggtatgcat gagaacaggc tttcctctca atctttcaag caaatgctgt cgggacagcg
      241 acgcacttct cgcttgaatc ggacacgact ctcaacatgt ttccctctca tcgaatacga
      301 actggtctgt tcctgggaca atgcgcctgg ggaattggtc ttgtcgctct gctggggtta
      361 cagaccttct atgaggtctc tggggagact gcggtgtatt gcctctcgct gttcctggtc
      421 ggtttaagcc tgtggtcgct ctggtcctgg aagaccgcga caggccattt gctggatttg
      481 tatagtctgt tccttatcgc ggttatgttg ttttcgggag gacagtcctt tctggaagtg
      541 ctggggttaa atacgcatgg tctccttggc ggaaaatttc ccattggagc agttcgtcag
      601 gcgctccttc ttgtcgtggc gagcatgctc gcgttccata ctggtgcgct gtacggtgca
      661 agatccagca tcaccacgat cgaccgatca cccgcagacg gtgctctcga gcaggctctc
      721 cgaacggtcg gcgccatgat catcatctta agtatcggac cggccatgta cgagctctat
      781 catgatttca acttggttca gaccctggga tatacaggcg aattcatgcg ggcgcgggaa
      841 gtgacgggtg cgaatgcctg gacgttggtt attgcgccgt ttttgattcc cggaacgctg
      901 ttgctcctgg ccggaagtcg gggacggcgc gggaacatca ttctgtcctg ggtgctgatc
      961 gcagtacctg cctgtatcta tgttctgttg ggaatgcgca accatgggct caaaggaatg
     1021 gtcgcgcttc tctggcttca cgctatggtc attggaccaa tcaagaaatc tgtcgtcgtc
     1081 ttagccgcaa cgattgtctt ggtcttggtc ccgggactgg ccgcagttcg aactgaacga
     1141 ttggttggaa tggataaaat tggcgcgttg gtggactcct atgaggcaat ggacaatccg
     1201 cttgtcctgg ctatgcggga gatgggcgga agtttcatta cgatcattta cacgaccgaa
     1261 ttcgttccac agatcagacc ctttgagtgg ggagcgacct acctcagtgc ggtgggcact
     1321 atcttcccga actttttctg ggatattcat ccgtccattg ccggcggact cccttcctac
     1381 tggctgatga aagaagtcga cccctatgcg gccaaggcag ggcagacgct tggattctcg
     1441 gttattgcgg aggcctatct caacttctca tgggtcggca cactcatatt catgagcgtg
     1501 attgggtatg tcctggctcg gtttgtgctg tgggtctgga aacaatctaa cccgctcggg
     1561 ttggccatcg aagcgatctt catttctttt gttttattcg ttgcgcgtgg tgaaagtcat
     1621 ctgatcgtgc gttccatctt ctgggaatgt ctcattccgt acctgttggt cacgtatctg
     1681 tataaccgcc aactcacccg ccagtgccac gcccagttaa tcgatgctcc atcctcttcc
     1741 ctaactgcgg caagctagac aaatttaggg tatcaatcag atgaggcctt tgcaaattct
     1801 gcacgtggtg ggtgctatga accggggagg agctgaaacc tggctcatgc atgtcctgcg
     1861 ggcaatcgac cgcgatcgat ttcacatgga cttcctggtt cacacggaca cgccagcagc
     1921 ctacgatgag gaaattgttt cattgggatc ccaggtatta tcctgccttt ctccgtctcg
     1981 accgtggatc tatgccagaa attttgcaag ggctgtccgt catgggttgc catatgacgt
     2041 cattcacagc catgttcacc attacagcgg cttcatcctt cgcttggctc accgatcgca
     2101 cattcccatc cgtcttgcac acagccacaa cgatacgtcc gaagtcgatc gctgttcagg
     2161 atgggccaga cgccaatacc tacacatctc gcaggagtgg atcaggcgct acgccacgac
     2221 tggtgtagct tgctccgccg aagccgggtc ggcactcttc agtccgagca ggggtacctc
     2281 gctggcatgg cggacattgc ccctcggcat agatctagcc tcattcgagg ccgggtcaga
     2341 ccgtgggcaa ctccgaagca gcctgggaat tccaaccgag gcattcgtga tcggtcatgt
     2401 gggacgtttc atggcacaaa agaatcatca tttcctggtc gacattcttt tggaaaccgt
     2461 caagcgccac ccgctcacgt ttgtgatcct cgtgggagac ggtcccttac gttcatctgt
     2521 ggagcgtaaa atcgaaacgc tcggtctcac aaaggtcgtc cgcttcttag gactccgttc
     2581 cgacgtgcct gagctgatgt tgagcgccat ggatgcgttg gtcttacctt cattgcatga
     2641 aggctttccg gtggtactgc ttgaagccca agcggccgga cttccctgtg tcgtctcaga
     2701 cgttgtcacg gcagaagtcg acgtggtcaa tcagctggtg tcacgcgtgg ctcttcagaa
     2761 ttcgcccgct gtttgggcgg acgccattct cgctacgcgc ggacggaaaa ctcggcacac
     2821 tcagcgcgcg gctttggata cgctttggca aagtccgtac agtattcagc attcggtcac
     2881 ggaacttcag cgactgtaca cggttctggc ggagcggcat gtcccttccg cttcccggac
     2941 gtactcgtaa ggtttggtat cggcctcttc cgcaattcta tgaaagtcac tgttaccact
     3001 gagcacagat tcatgaggac gcccgacgga agggtctgga catgcgccca gtatggctat
     3061 gcgttctttc ttcgttatct ggaagtattc gatcacgtcg aggttgtctc gcgagtctgt
     3121 gatgtgcctg tcgttccctc gcattggcag cgtgtcgatg gatcgggggt atcgctcatc
     3181 ccagtgccat actacattgg cccgtggcag tatctgcggc ggcgcccgcg catcatgcgc
     3241 gtgtttttgg cggcggcaaa gactgctgag gctgtgatct tcagaggagg gtcttccttc
     3301 agtgacgggt tgacccgcgc ttggcgaaca cggcctcgac cgtttggcgt ggaggtcgtc
     3361 ggggatccat gggaggtgtt cagtcccggc gctttcacgc atcccctgcg tcccttcttc
     3421 cgcctgtggt tctaccggca acaggcccga ctgtgcttga aagcgagcgc cgtggcctat
     3481 gtgacgaagc atactttgca acgccggtat cctgcccgcg ccggagcgct ctcggcgagt
     3541 tattcagacg tggaactaga caacgacgcg tttgtctcaa gtccacgggc atcttctctc
     3601 gacggaactc ccattaccct cctcactgtt gctactcttg cgcaattgta taaaggtcag
     3661 gatacgttga tcagcgccat ggcgcgttgt gtccgccaag gcctcgactt acatctcgtc
     3721 ctggtcgggg acggacacta tcggagccgg ttggaaaacc aggctgccga tctcgggctg
     3781 ggcgagcgca ttcggtttct cggtgaactt ccctcagggg ctgcagttcg agctgaactc
     3841 gatcgagcgg atctcttcgt attgccgtca cgcactgaag gcctgcctcg tgctctcgtc
     3901 gaggccatgg caagaggatt gccctgcgtg ggatcgacgg ccggggggat tcccgaactc
     3961 ttagaaccct gcgatctggt ccggccaaac gatgttcagg cactggccga aaaagttcgg
     4021 gaggtcgtca cccagcctca ccgtagggca gagatggcgc tcagaaatct tcagcgtgcc
     4081 cggcagtatc acgagactct gctccgtaag actcgcgaag acttctaccg ctatgtcaaa
     4141 gaccgtacag catacgccag gccgctccga ccctgacgcg ggattcaggc gccccccgat
     4201 cttttccggc tgcagtattg ccgctgacat tggcgaaggt ccttggaggc ggttcgacca
     4261 ctattccata cgagtatcga cgacgcaatc atggctccca ctcctatcag gttgattcac
     4321 atcacgactg tcccccagtc gctcttcttc gtttccgggc aagcggggta tatgaaagct
     4381 cggggtttcg aggtccatgg cctctcttcg ccgggcggac atctttccgc atttgccgcc
     4441 agtgaaggag tcgatgtcga agcgatagag atgccgcggc gcatcacgcc tctgcaagat
     4501 ttgatcgcga tttctaaaat atgcctgagc tttcggcgtc tgcgcccgca tatcgtccat
     4561 gcgcatactc caaaaggggg cctcctcggt atgctcggcg catgggtgag ccgcgttccc
     4621 atacgtgtct accatttgca cggactgccc tatctcacgg cgaccggatg gagacgaagg
     4681 ctcctgatgg gatccgaata cctctcctgt aaacttgctc accgcgtgat ctgcgtgagt
     4741 gcctcagttc gtgccgttgc tgtggacgcg ggattatgtc cagccgacaa gattcgcgta
     4801 ttgcttcagg gaagttgcaa tggcgtcgac gcggccgtgc ggttcaaccc ttcccggctc
     4861 ggagacgaag ttcggaacgt aacgaggcgt aagtacgata ttccatgcga ggctaccgtg
     4921 gtaggattcg tcggccgtct ggtccgcagc aagggcatcg ttgagctggc cgatgcgtgg
     4981 agcaccctgc gtcaggaatt tccgaggctc catctattgc ttgtcggccc tgctgagccg
     5041 ggggatccgg ttccttcgga agtcgagcgg cgccttcaga acgacccgcg cgttcatttg
     5101 gttggagagg aatgggatat tcctccggtg tacgccgcca tggactttct gattctgccg
     5161 acttaccgag agggttttcc tatcgtgttg ctcgaagccg cagcgatggg actggctatg
     5221 gtcgcgacga ccgtgcctgg atgcgtcgat gccgttcagg atggtgtcac cggcacactg
     5281 gtcccgccgt atgactcccg acggctcgct gcggcagtgc gcggttacct gaaagatcct
     5341 gaactgagtc gccggcatgg cgaggccgct cgcgaacggg tgttacgcga tttctcagat
     5401 cgggccatgt ggaaagccgt ccacgaagag tacgtcacat tacttaaaaa acagggcctc
     5461 gcggttcccg atgccgctac gccctccacc tccgcagatt cgtctgtaaa catagacgtc
     5521 cgtaatcatc gactgaccaa accttccggc tcgtagggtc catgctccta tacgtatgag
     5581 cgtgacgatt gtcgcgagaa tcttctggaa ccatgcgcca tcaagactca tcacggacag
     5641 ccgttccgtc tcttcctaag cgtctcttcg acctggtcgt cactgccagt gcgataccac
     5701 tcgtcctgcc ggtcatcgca ggccttgccc ttgccgcatg gaccatccat ggatctccgg
     5761 tcttgtttac gcagccgcgt cccggcctcc atggacgtcc gttcattttg atgaaattca
     5821 gaacgatgaa gaaggcctgc aattccgggg ggcgcccgct gtccgatgcc gagcgcctga
     5881 ctcgattcgg gcgattcctt cgcagcacga gcttggatga gcttccagaa ttggtgaatg
     5941 tcctgaaggg cgaaatgagc ctggtcggcc ctcgaccgct tttaatggaa tacctggaag
     6001 aatatactcc cgaccaacat cgtcggcacg aggtattgcc tggaattacc ggatgggcgc
     6061 agatcaatgg acgcaatgca ttgacctggg agcagaagtt tgcgctg
//
LOCUS       DAORQN010000079         6041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_575_length_6041_cov_7.635650, whole genome shotgun sequence.
ACCESSION   DAORQN010000079 DAORQN010000000
VERSION     DAORQN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_575_length_6041_cov_7.635650"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>304)
                     /locus_tag="PK614_05885"
     CDS             complement(<1..>304)
                     /locus_tag="PK614_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81776.1"
                     /translation="LTETAQLQKNLKGRLYQHYDEAGRLQAEQCDFKGNLTTKFRQVI
                     QDSELTKAYAMPPYVAYRVDWTPQGVDTAASRGQRAAALLAQIRFDTSTSFAALNRI"
     assembly_gap    305..328
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<329..5112)
                     /locus_tag="PK614_05890"
     CDS             complement(<329..5112)
                     /locus_tag="PK614_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HRB81777.1"
                     /translation="MAGQASAPNQIISLPKGGGAQKGIGEKFSPDLHTGTGNFTVPLA
                     LPPGRNGFQPQLNLAYSTGTGNGVFGLGWALSIPGITRKASKGIPRYRDYDLDIKKRD
                     TFILSGAEDLVPIQDSSLDPTVATRYRPRTEGLFAKIVHHHDQLHGINYWEVCSKDGL
                     ISYYGINPAEPQTDDPTTYDQPFRTRTTPATITKPKLNVADPDRVFAWKLTLTKDPFG
                     NRIEYLYETRDQSDEADERQGHQWDQPLLTQIRYADYKQNPTKFLVTVTFDYEARLDS
                     CSDYRAGFEIRTSARCRSILVTTHADKDYPVRRYDLRYENETLNKVSLLTSVDVIGFD
                     DAGNEAKELPPLQFSYSRFNPQDQQQRDFYPVEGADLPATSLANTSMELVDLFGNGLP
                     DILEMNGTVRYWRNRGGGRFDLPKPMAEAPAGLALGDPGVQLIDANGDGRSDLLVTQG
                     TIAGYYPLLFNGRWDRRSFQKYRTAPSFDLKDPEVRLLDLTGDGVTDCLRSGTSFECF
                     FNDPLDGWNETRRVQRQGLTDFPNVNLSDPRVKLADMSGDGMQDIVLVHDGNVEYWPN
                     LGYGNWGKRLHMAKSPRFPYGYDPKRILVGDVDGDGFADILYIDNAKVTLWINQSGNG
                     WSEPIEILGTPPVTDMDSIRVIDLLGSGISGVLWTRDASSSRQDHYLFLDLTGGTKPY
                     MLQEMNNNMGAVTKVSYAPSTEFYLDDEPKPALRWRTPLPFPVQCVARVEVIDALSQG
                     KLTTEYRYHHGYWDGAEREFRGFGFVEQLDTETFDRYNERGLHGRDVSFIEIQDRARF
                     SPPMLTKTWFHQGPIGEEFGDWEEHDWSTDYWTGDPPLLAHARDLNDVLTVLDLTYAG
                     KPTRERRRIKRDALRTLRGSILRTELYALDGSPQQGRPYTVTEQAYSLSEIDQPASAE
                     PTRLHIFFPHAAAQRTTQWERGDDPMTQFSFTEDYDAFGQARSQTTIACPRGWRRMSD
                     RHTDYLATRTRTGFAEPSDPTATYLYDRVAKSTTYEIRNTSARNVLECRDLKDTDPAL
                     VIIGHTVNWYDGAAFEGAVFGAVQKYGALVRSETLVLTRNILQESYKSADRLSAAPEE
                     PAYLRTDGNAIAWTQDYPQEFQDRLPPLAGYLYRTATDGGHYVDGFYTATERRQYDFH
                     LAHNGEGKGLFVTSRDPMGHDTRVVYDNYLLLPSKVTDAVGMMTTAIYNYRVLQPTTV
                     IDPNNNKTAFTFTPMGLLKETWLKGKDDAARPEGDRQEPSAKLLYDFWAFVRRKEPIS
                     VQTIRRVHHDTEIDVPAGSGEGQRDETIETREYSDGFGRLLQTRTQAETVLFGDNVFG
                     GGILPENQADEHSTRSSVQGTTNVDPAEPNVVVSGWQTYDNKGRVIEKYEPFYAKGWD
                     YQPIQQRQLGQKVTMYYDPRGQVIRTVNPDGTEQRVIFGIPTALDRPDQVTATPWERY
                     TYDANDLAPISSQQRPDGTIVSLSSRASATHHYTPASALLDALGRVVETVERNGVTAA
                     DRLIIRTPYDIRGNVLTITDSLGRLAFRHVYDLANRPWRIESLDAGLRRMVLDAIENE
                     VERRDSKGSVSVRAYDQLHRPTHLWAKGATQDPVTLRERLIYG"
     gene            5449..>6041
                     /locus_tag="PK614_05895"
     CDS             5449..>6041
                     /locus_tag="PK614_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81778.1"
                     /translation="MKGKYEVTGIGNKALVPDQIHITRRSDKELALRFLRDGKWDEII
                     VPSAKLGEKDVLPDPIKGVNIGRKPTDSKSVSVKNYEELLLGRRPSSTEPPPKLRLIY
                     SKTTISPIGGIGSRGLQFTVSGVALQSIIAKEIRITRYSDKALTIKMLVGESKWQEIQ
                     ATSLELDGKIVVPGPEKGIVLRGETESRGYQEIVLGRT"
BASE COUNT         1245 a         1616 c         1712 g         1444 t
ORIGIN      
        1 aatgcgatta agggcagcga agctcgttga ggtgtcgaaa cggatttggg ccaatagtgc
       61 tgcggcgcgc tgcccgcgac tcgctgcagt gtcgacacct tgcggcgtcc agtccacgcg
      121 gtaggcgacg tagggcggca ttgcatacgc ttttgtcagt tcggaatcct gaatgacctg
      181 gcggaacttg gtagtgaggt tacctttgaa gtcgcattgc tcggcttgca gccgtcctgc
      241 ttcgtcgtaa tgctgataca gtcttccttt gagattcttc tgtaattggg ccgtttcagt
      301 caggnnnnnn nnnnnnnnnn nnnnnnnncc atagatcagc cgttcgcgca aggtgaccgg
      361 gtcttgggtc gcgcctttcg cccagagatg tgtcggacgg tgcaattgat cgtatgcccg
      421 tacgcttaca cttcctttgc tgtctctccg ttcaacctcg ttttctatag catctaacac
      481 catgcgacga aggcccgcgt cgaggctctc gattcgccag ggtcgattgg ccagatcgta
      541 gacatgccgg aaggcgaggc gaccgagcga atcggtgatc gtgagcacat ttcctcgaat
      601 gtcatagggc gttctaatga tgagacgatc ggcagctgtc acaccatttc gttcgacggt
      661 ctcgaccacc cgtcccaacg catcaagcag cgcactggcc ggggtgtagt ggtgggtggc
      721 ggacgctcta ctcgagagcg agacgatggt gccgtcaggt ctctgctgac tactgatcgg
      781 agcaaggtcg ttggcgtcgt aggtgtagcg ttcccagggc gtagctgtta cctgatcggg
      841 acgatctaat gctgtcggta tcccgaagat gacgcgctgt tccgtcccgt ccggattgac
      901 ggtgcggatg acctgcccac gtggatcgta gtacatcgtg actttttggc cgagttgcct
      961 ttgctggatc ggctgatagt cccatccctt cgcataaaat ggctcgtact tctcaatgac
     1021 gcgccccttg ttatcgtagg tctgccagcc gctgacgacg acattcggct ccgctgggtc
     1081 aacattcgtt gtgccctgta cgctcgatct tgtgctgtgt tcatcggcct ggttttccgg
     1141 gagaatccct ccaccgaaca cgttgtcgcc aaagaggacg gtttcggctt gcgtcctcgt
     1201 ttgcagcagt cggccaaagc cgtccgagta ttctctcgtt tcgatggttt cgtctcgttg
     1261 gccttcacca gacccggccg gcacatcgat ttccgtatcg tggtggacac gcctgattgt
     1321 ttgaacagaa atcggctcct tgcggcgcac aaacgcccaa aaatcataga ggagcttggc
     1381 gctgggctct tgccgatcgc cttcggggcg cgccgcatca tccttccctt ttaaccaggt
     1441 ctctttcaat agtcccatcg gcgtgaaggt gaaggccgtt ttgttgttgt tcgggtcgat
     1501 gacggtcgtt ggttgcagaa ctctgtagtt gtatatggcg gtggtcatca ttcccaccgc
     1561 atcggtgacc ttggatggga gcagcaaata attgtcgtac acgaccctcg tgtcatggcc
     1621 catggggtcg cgcgatgtga caaacaaccc cttcccctcg ccattgtgag ccaggtggaa
     1681 atcgtattgg cgacgctccg tggcggtata gaagccgtcg acatagtgac caccgtctgt
     1741 tgctgtccgg tacaggtacc ccgccagcgg tggtagccgg tcttgaaact cttgtggata
     1801 gtcctgcgtc caggcaatgg cattgccgtc agtccttagg tatgccggtt cctcaggagc
     1861 ggcgctgagc cggtcggcac tcttatagct ttcttgcagg atgtttcgcg tgagcacgag
     1921 tgtctcggac cgcacgagtg ccccatactt ctgcactgcg ccgaaaactg ctccttcaaa
     1981 ggccgcaccg tcgtaccagt tcaccgtatg cccgatgata accagcgccg gatccgtgtc
     2041 cttgaggtcg cgacactcca acacattgcg ggcagaggtg ttccggattt cgtatgtggt
     2101 cgacttcgcc acgcgatcat acagataggt tgcagtgggg tctgagggct ctgcaaatcc
     2161 ggtgcgggtc ctggtcgcga ggtagtcggt atgtcgatcg gacatacgtc gccaccctct
     2221 tgggcaggca atcgtggttt ggcttcgtgc ctgtccgaac gcgtcgtagt cttcggtgaa
     2281 ggagaattgg gtcatcggat cgtccccacg ttcccattga gtcgtccgtt gggcggcagc
     2341 gtggggaaag aagatatgca gcctcgtggg ttcggcagag gccggctggt cgatttcgct
     2401 caggctgtag gcctgctctg tcaccgtata ggggcggccc tgctgagggc ttccgtcgag
     2461 ggcatagagc tctgtccgaa ggatgctgcc tctaagagtc cgcaacgcat cgcgcttgat
     2521 gcgtcggcgc tcccgagtgg gtttccctgc ataggtaaga tcgagcacgg tgagcacatc
     2581 gttcagatct ctcgcatgcg cgaggagtgg aggatcgcca gtccaatagt cggtggacca
     2641 atcgtgttcc tcccagtctc caaactcttc accgatcggg ccctgatgaa accacgtttt
     2701 cgtcagcatc ggcggcgaga agcgcgcgcg atcctgaatc tcgataaagc tcacatctcg
     2761 gccatggagg cctcgctcgt tgtaccgatc gaaggtttcg gtatcgagct gttcgacgaa
     2821 gccgaacccg cggaactctc gctccgcgcc atcccagtag ccatgatggt atcggtattc
     2881 ggtcgtgagt ttgccttgtg acaatgcatc aatcacttcg acgcgcgcga cgcactgcac
     2941 gggaaacggt agcggtgtcc gccatcgcag tgccggcttg ggctcgtcat cgaggtagaa
     3001 ttccgtcgaa ggtgcatagg acaccttcgt gacggcgccc atgttgttgt tcatctcctg
     3061 cagcatgtaa ggctttgtcc cgcccgtaag gtcgaggaac agatagtggt cctggcggga
     3121 ggaactcgcg tccctcgtcc acaggactcc gctgatgccg ctgcccagca gatcgatcac
     3181 acggatgctg tccatatcgg tgaccggtgg tgtgccgaga atctcgattg gctcgctcca
     3241 cccatttccg ctttggttga tccagagcgt gaccttggcg ttgtcgatat acagaatgtc
     3301 agcaaagcca tcaccatcca catcgccaac cagaatgcgt ttgggatcgt acccataagg
     3361 aaaccgggga ctcttggcca tgtgtagccg cttgccccag ttgccgtacc cgagattggg
     3421 ccaatattcg acgttgccgt cgtggacaag cacgatatct tgcatgccgt ccccagacat
     3481 gtcagcaagc ttcactcgtg gatcggacaa attgacgttg gggaaatcgg tcaatccttg
     3541 ccgctggact cggcgggtct cattccagcc atccagcgga tcgttgaaga aacattcgaa
     3601 agatgtacct gaccgaagac aatcggtcac gccgtcaccg gtgagatcga gcagacggac
     3661 ttccgggtcc ttgagatcaa atgagggcgc cgtgcgatat ttttgaaagg agcgtcgatc
     3721 ccaccgtccg ttgaatagca ggggatagta gccggcgatt gttccttgag tgaccaagag
     3781 gtccgatcgg ccatcaccat tcgcatcgat aagttgtacg cccggatcgc cgagggctag
     3841 cccggctggt gcttccgcca tgggcttggg cagatcgaat cgaccgccgc ctcgatttcg
     3901 ccaatatcgg actgtcccat tcatttcgag gatatccggc agtccatttc cgaagagatc
     3961 caccagttcc atcgaggtgt tggcaagcga cgtagcagga aggtcggctc cttcgactgg
     4021 gtagaaatcg cgctgctgtt gatcttgggg attgaaccga ctgtagctga actgaagcgg
     4081 cggcaattcc tttgcttcat ttcccgcatc gtcaaagccg atgacgtcga cggaggtgag
     4141 cagggatacc ttgttgagcg tctcattctc gtaccgcaga tcgtagcgcc taacggggta
     4201 atctttgtct gcgtgggtcg tcacgaggat cgaccgacat cgtgccgacg tccggatctc
     4261 gaaccccgcc cggtagtctg aacaagaatc tagccgagcc tcgtagtcga acgtgacggt
     4321 caccagaaac ttcgtcggat tctgcttgta gtcggcatag cgaatttggg tcagcagtgg
     4381 ttggtcccac tggtgcccct gccgctcgtc agcttcatcg ctctgatcac gtgtttcata
     4441 gagatattcg atccggttgc cgaacggatc tttggtgaga gtgagcttcc aggcaaagac
     4501 tcgatccgga tcagctacat tcagttttgg ttttgtgata gtcgccggtg tggtacgcgt
     4561 tctgaatggc tgatcatagg ttgttggatc atcggtctgt ggctctgccg ggttaatccc
     4621 atagtagcta atcagtccgt ctttactgca cacctcccag tagttgattc cgtgcagttg
     4681 atcgtggtga tggacgatct tggcaaagag cccttcggtt cgagggcgat agcgtgtggc
     4741 cactgttgga tcgagggaag agtcttgaat cggaacgaga tcttctgctc cagagagaat
     4801 aaacgtgtct cgttttttga tatcgaggtc gtaatctcgg tatcgcggaa tgcccttcga
     4861 cgctttgcgg gtgatacccg ggatgctcaa agcccagccg aggccgaaca caccattgcc
     4921 ggttccggtg ctataggcaa gattcagctg tggttgaaac ccattacgac caggaggcaa
     4981 ggcaaggggg actgtgaaat tacccgtccc tgtgtggaga tcaggcgaga atttttcgcc
     5041 gatgcctttt tgcgcgcccc cgcccttcgg gagagaaatg atttggttgg gggcactcgc
     5101 ttggccagcc atgagattga atgttctcca tcaaacgaac cggcgctggc aagagtgcaa
     5161 caatttaaat gactggtcaa cagaaaagtt ccagtaaatg cagtttttat acgtacaaat
     5221 aaaatacgtg catttaattc tgctgacttg cctggaataa ttgcttatgc tgacttcact
     5281 tgaagaagca ggcgcactct gacacctatc accgaggagt gaatcatgcg agtaccatgc
     5341 cgtcgatgtg ttctcacact gacgctcggt cttcttcttg gattaactct gcacgacatt
     5401 gcgtggggac tcatcattcc acgccttaag cccatgactt cgcccggcat gaagggaaaa
     5461 tacgaagtca ctggtatcgg caataaagca cttgtcccgg atcagattca catcactcgc
     5521 cgatcagaca aagagctcgc tcttagattt cttcgggatg gcaagtggga cgaaattatc
     5581 gttccgtcgg ccaagctggg agagaaggat gtcttgccag acccaatcaa gggcgtcaat
     5641 atcggaagaa agcccacaga cagcaaatca gtttctgtca agaactatga agaactatta
     5701 ctcggccgcc gcccaagttc aacagagcct cccccaaaac tccggctaat atattccaag
     5761 acaactatct caccaatcgg cggcatcggt agtaggggac tgcaattcac agtgagcgga
     5821 gtggccctcc aatcgatcat cgctaaagaa atccgaatca ctcgttattc cgacaaagct
     5881 ctgacgatca aaatgttagt gggtgaatct aagtggcaag agatccaagc cacgtcttta
     5941 gagttggatg gaaaaatagt ggtaccggga ccggaaaagg gtattgtcct gcgaggagaa
     6001 accgaatcac gcggatatca agaaatcgtt ttgggtcgaa c
//
LOCUS       DAORQN010000080         5971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_576_length_5971_cov_13.532623, whole genome shotgun sequence.
ACCESSION   DAORQN010000080 DAORQN010000000
VERSION     DAORQN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_576_length_5971_cov_13.532623"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="PK614_05900"
     CDS             <1..1876
                     /locus_tag="PK614_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB81779.1"
                     /translation="HPEDQAAILTNLAQVLQSDQNLWTAEYRFRRADGTYAYVIDHGY
                     VLRDHDGHPYRMIGAKADITERRQAETMHAVQLAVGLALEESASLSEAVPKILRTMCE
                     LQGWTLGALWLVHSQDKTLRCNALWHQPGLPAENFARIYRGLALRQGAGLAGQVWNTG
                     DATLCPDILNQADCPLLDAAQKADLHGGIAFPIHTEKEILGIMEFLTYERLRPSSSRL
                     ERASELGAMITQFLHRKDLERQLRQAQKMEALGRMAGGIAHDFNNLLTIINSWAELLT
                     NEPGQTTRTQRGMAQIREAGNKATALTRQLLAFTRHQIVERQPLNLNDRVTDIVELMK
                     RVIGEDIQLALTLDPALGRIKADPGQIEQVVMNLVVNARDSMPQGGRLDLETKSVSIT
                     HSDPLWPDPLTPGPYVTLAVRDTGCGMDADTVAHVFEPFFTTKEPGKGTGLGLSTVYG
                     IVRQSGGTVGIESEPGRGTTFTIYLPCIDEDSESPRPVAQLMSIIEQSETILLVEDND
                     MVRGLAQTVLVGQHHSVLAARTGEEALQLVRQRGGKISLLITDMVMPGMGGIQLAAEL
                     RTLQPEINIILTSGYSDREGSMLEQLDARTTFLPKPYTPDSLIKAVNAALDPTLSNQT
                     "
     gene            complement(1948..2934)
                     /locus_tag="PK614_05905"
     CDS             complement(1948..2934)
                     /locus_tag="PK614_05905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRB81780.1"
                     /translation="MSFLDRFLIISVVLLTSVFPVELFPNTGPVPRGGDGQFSGKQGQ
                     VILINVPDIKDAASVKGQFLGRKVTLFPDPSAGGGAGYVGLLGIDLQDEPGAHELTID
                     AQLGEQMRHFSYQVLVLKEKFAVEHLKLPKDKVDLDEKAAARWKAEQEQVRKALAEES
                     AMRLWQAGFIEPVHGKRTGIFGSVRIMNGQARNPHNGEDIGAPMGTDVMASNDGVVRL
                     IVDHIFSGRGIFVDHGLGLYSMYFHLSDVLVKEGDLIKAGQIIGKVGATGRATGPHLH
                     WGMKVNGARVNPYALLELPFPQNPAVARPVLASPTRPAQGESSATAVGGDTR"
     gene            complement(2967..4343)
                     /gene="pafA"
                     /locus_tag="PK614_05910"
     CDS             complement(2967..4343)
                     /gene="pafA"
                     /locus_tag="PK614_05910"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HRB81781.1"
                     /translation="MQQRIFGLENEYGLIFSPNGKIYLPMEKVLGYIFEGLIPNSWPS
                     NAFLVNGARFYQDTGCHPEYSTPECDNILDLVIHDKAGERLLEACLPAAEERLREEGL
                     SGEIYIFKNNTDSLGNTYGCHENYLMRRDVDFWKVTEQLIPFFVTRQIYSGAGKVLKV
                     SGKPQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIIVGDSNMSEY
                     ATYLKVGTATLVLSMIEAGYSVSGMELEDPVKAIREISRDPTLNKKVKLDDGRQMTAI
                     EIQRVYVKRAKEFLSEQEHDPVLDDVWEKWVSVLDRLEEDPMQLVHEVDWVMKKHLIQ
                     SYTDKKDCGWDDPRVFLLDLQFHDVKRTRGLYYLMENRGMAARVVEEEAVQRAMSVPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRVEELLSQ
                     VSGARLYR"
     gene            complement(4476..5159)
                     /gene="prcA"
                     /locus_tag="PK614_05915"
     CDS             complement(4476..5159)
                     /gene="prcA"
                     /locus_tag="PK614_05915"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HRB81782.1"
                     /translation="MPLPYYVSPEQMMQDKAEYAKKGIAKGRSIIALEYIDGILLAAD
                     NPSSSLHKVSEVYDRIAFAGAGKYSEFEHLRKAGIRHADLTGYMYSREDVSARTLSNA
                     YSQSLGTAFSTDVKPLEVEILVVQVGVNGQANEMFRISFDGSIVDEKNLAVIGGRSEA
                     VQQYLREHATPQPPTLKDGLRRCLAALEQVATQKIPSENLEVAVLDRNRLGRKFKRLS
                     SADISQLFA"
     gene            complement(5163..>5971)
                     /gene="prcB"
                     /locus_tag="PK614_05920"
     CDS             complement(5163..>5971)
                     /gene="prcB"
                     /locus_tag="PK614_05920"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HRB81783.1"
                     /translation="FSHHEGSSFFDFLTLHHPELRPGLSEIAIQRAALPVDFGRPGTI
                     PVPHGTTVLALKYRDGAIIAGDRRATEGFQIADRRIEKVFRIDDYSAMAIAGAAGPCI
                     EMAKLFQTELEHYEKLEGVQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYVGYDVKR
                     NEGRIFKYDITGGRYEESDHHSIGSGGKDARNTMREHYRSGLQEEEALRVGLLALYNA
                     ADEDVGTGGPDLVRGIYPTAKIVSSAGITDVPEDRVRALFETMIAERRRS"
BASE COUNT         1275 a         1837 c         1636 g         1223 t
ORIGIN      
        1 tcatcccgag gaccaggccg cgatcctcac caacctcgct caagtgctcc agtcggacca
       61 gaacctgtgg accgccgaat atcgattccg tcgagccgac ggcacctatg cctatgtcat
      121 tgatcacggg tatgtgctcc gggaccacga tggccatccc taccgcatga tcggcgccaa
      181 ggccgacatc accgaacgac ggcaagccga aacgatgcat gccgttcaac tcgcggtcgg
      241 cctcgcgctt gaagaatccg cctctctcag tgaagcggtc ccgaaaattc ttcgcaccat
      301 gtgcgagctc caaggctgga cgctgggcgc actgtggctg gtgcactcac aagacaagac
      361 cttgcgctgc aatgccctgt ggcaccaacc gggcctccct gccgagaact tcgcccgcat
      421 ctatcggggc cttgccctgc ggcagggagc cggtttggcg ggccaggtct ggaacacagg
      481 ggatgccact ctctgcccgg atattctcaa ccaggccgac tgcccgctcc ttgacgctgc
      541 gcaaaaagcc gacctccacg gagggatcgc gttccccatt cacacggaaa aagagatcct
      601 ggggatcatg gagtttctca cctacgaacg gctgcggccc agctcatctc gattggaacg
      661 ggcttctgag ttgggggcca tgatcactca atttcttcac cgaaaggatc tcgagcgcca
      721 gcttcgtcag gcgcaaaaaa tggaagccct gggacgcatg gccggaggaa ttgcacacga
      781 cttcaacaat cttctgacga ttatcaatag ctgggctgag cttttgacga atgagccggg
      841 acaaaccacc cggacgcagc gcggaatggc acaaatcagg gaagccggca ataaagccac
      901 tgccctcacc cggcagttgt tggccttcac acgtcaccag attgtggaac gccaaccgct
      961 caacctgaat gaccgcgtga ccgacattgt cgagctcatg aaacgtgtca tcggggaaga
     1021 cattcaactc gccctcacct tggatcctgc acttggacga atcaaggcgg atcccggcca
     1081 aatcgagcag gtcgtgatga atttggtggt caacgcgcgc gattcgatgc cgcagggagg
     1141 ccgcctggac ctggagacga aatcggtctc gatcactcac tccgatccgc tctggcctga
     1201 tcccctcacc ccgggcccgt acgtcacgct cgccgtccgg gacacaggat gcgggatgga
     1261 tgccgacacg gtcgctcatg tgtttgagcc gtttttcacc acgaaggagc cgggcaaagg
     1321 taccggcctc ggattgtcca cggtctacgg aatcgtccgg cagagcggag ggacggttgg
     1381 gattgagagc gagcccggac gtggcaccac atttacgatt tatctgccgt gcatcgacga
     1441 ggattccgaa tcgccgcgcc ctgtggctca actcatgagc atcattgaac agtcggaaac
     1501 tattctcctg gtcgaagaca atgacatggt tcgaggcctg gcacagacgg tgctggtcgg
     1561 acagcatcat tcggtactgg ccgcgcgaac aggtgaggaa gcgctgcagt tggttcgcca
     1621 gcggggaggg aaaatctcac tcttgattac cgatatggtg atgcccggca tgggggggat
     1681 acaactcgcc gccgaactcc ggacgctgca accggagatc aacatcatcc tcacctccgg
     1741 ctattcagac cgcgagggat cgatgctgga acagcttgat gcacggacca catttctgcc
     1801 caagccctat acccccgact cgctgataaa ggcagtgaac gccgcgcttg atccaacgtt
     1861 gtcgaatcag acctgaggga aacggctcac ggatcctgag aattgatccg ccggctggat
     1921 gggacggcgg gtcagcagcg agtcgcgtta tcgcgtatca ccgccgactg cggtggcgct
     1981 tgattcgccc tgtgcgggac gcgtcgggga ggccaacaca ggccgtgcaa cagcggggtt
     2041 ttggggaaag ggcaattcga gcagagcgta gggattcaca cgcgccccgt tgaccttcat
     2101 gccccaatgg agatgcggac ccgtggcgcg accggtggcg ccgactttgc cgatgatctg
     2161 cccggccttg atcaaatcac cttctttcac cagcacgtcg gagaggtgaa agtacatcga
     2221 gtacagaccg agaccatggt ccacaaagat gccgcggcct gaaaagatat ggtccacaat
     2281 gagccggacg acaccgtcat tgctggccat cacgtcggtg cccatcggcg cgccgatatc
     2341 ttctccgttg tgcgggtttc tggcctgacc gttcatgatg cggacgctgc caaaaatccc
     2401 ggtccgctta ccatggaccg gttcgatgaa cccggcctgc cagagccgca tggcagactc
     2461 ttcagccaac gccttgcgaa cctgctcctg ctcggccttc cagcgcgcag ccgctttctc
     2521 gtcgagatcg actttgtctt tcggcaactt cagatgctcg acggcgaact tctccttcag
     2581 caccaacacc tgataggaaa aatggcgcat ctgctcaccc aattgggcat cgatcgtgag
     2641 ctcatgcgca cccggctcgt cctgtaggtc gatccccagc aatccgacat aaccggcacc
     2701 gccaccggca gaagggtctg gaaagagcgt caccttgcgc ccaagaaact gacctttgac
     2761 gctggccgca tccttgatgt ccggcacgtt gatgaggatc acctggccct gcttgccgct
     2821 gaattgcccg tccccgccac gaggcaccgg ccccgtgttc ggaaaaagct cgacgggaaa
     2881 cacactcgtc agcagcacga ccgaaatgat gagaaagcga tccaggaacg acatggtctt
     2941 gtctcgtctc tgcccgtgcc cctctgtcat cgatatagtc gcgcgccgga cacctgcgat
     3001 aacaattctt cgacccgccg gttcaccgcg ctgaaaggat ccatacagag aatggtctct
     3061 tcccagtacc cgttcaactt caaatacgtc cagtcgaccg tgtacgaccg gttcttggcc
     3121 cgcgcgaacc ggataaagtc gccgcggact tttgcgcgcg tggtctgagg cggcacggac
     3181 atcgcccgtt gcaccgcttc ctcctccacg acccgcgcgg ccatccctcg attctccatc
     3241 agataataca gcccccgcgt gcgtttcacg tcatgaaatt gcaaatccaa caggaacacc
     3301 cgggggtcat cccagccgca atccttcttg tcggtgtacg actgaatcag atgctttttc
     3361 atcacccaat cgacctcgtg cacgagttgc atcggatcct cttccagacg atccaacacc
     3421 gacacccact tctcccagac gtcgtccagc accggatcgt gttcctgctc cgacagaaac
     3481 tctttcgcgc gcttgacgta gacccgctgg atctcgatgg cagtcatctg ccgaccgtca
     3541 tccagcttga ccttcttgtt gagggtcgga tcgcgggaaa tttcacggat ggctttcaca
     3601 ggatcctcga gttccatccc gctgaccgag tacccggctt cgatcatcga gagcaccaac
     3661 gtggcggtgc cgaccttcag gtacgtcgca tactccgaca tattggagtc gcccacaatg
     3721 atgtgcagcc gccgatactt ctccgcatcg gcgtggggct cgtcacgggt gttgatgata
     3781 ctccgggacg aggtcgtcga ggaggaggtc ttctcgtgaa tatgctgggc gcgctgcgag
     3841 atgaagtact gcggtttacc tgagactttc aggaccttac cggcgccgga atagatctgc
     3901 cgtgtcacaa aaaacggaat gagctgctca gtgactttcc aaaaatccac gtcccggcgc
     3961 atgagataat tttcatggca cccgtaggta ttgccgagcg aatcggtatt gttcttgaaa
     4021 atataaattt ctccggacaa cccctcttcg cgcaggcgct cttcagccgc cggcaaacag
     4081 gcttccagca agcgctcgcc cgctttatcg tggatgacga gatcgaggat attgtcgcac
     4141 tccggcgtgg agtactccgg atggcaacca gtatcctgat aaaagcgcgc gccgttgacc
     4201 aggaatgcat tcgacggcca gctgttcgga atgagacctt cgaagatgta gcccagcact
     4261 ttctccatcg gaagataaat cttcccgttc ggagagaaaa tcaggccgta ctcgttttct
     4321 aaaccgaaga tacgttgctg catggagaga cgtttcctcg aatccgcttc tctcaggata
     4381 caccgcggcg ggtgaggctt caacagatgc ccggcaatga aaagcgtctc accgaccgga
     4441 aagcggataa gcacgcagtt gcgtggaggg tattgtcagg cgaagagctg ggagatgtcg
     4501 gcactgctca accgtttgaa cttccgccca aggcggttcc ggtccagcac ggcgacttca
     4561 agattttcgg aagggatctt ttgagtggcg acctgctcca gggcggcaag gcaacgccgg
     4621 agcccatcct tcaacgtggg tggctgcggt gtcgcatgtt cccgcaaata ttgctgaacg
     4681 gcttccgacc gtcccccgat caccgcgaga tttttttcat ccacgatgct gccgtcgaat
     4741 gaaatgcgga acatctcatt cgcctgaccg ttcacaccga cctgcacgac aaggatttcg
     4801 acttccagcg gcttgacgtc cgtgctgaag gccgtcccca gactctgcga ataggcattg
     4861 gacaaagtcc gcgcgctcac atcttcccgg ctgtacatgt accctgtcag atcggcatgg
     4921 cgaatgccgg ccttccggag atgctcgaac tcgctatatt ttccggctcc ggcaaacgcg
     4981 atccggtcgt agacctctga cactttatgc aatgaggagc ttggattgtc cgctgcgagc
     5041 aggatgccgt cgatatattc aagcgcaatg atggagcgtc ctttggcaat accttttttg
     5101 gcatactccg ccttgtcctg catcatttgt tcaggcgaga cgtagtaggg caacggcatc
     5161 ggctaggatc tccttcgctc tgcgatcatg gtttcgaaca aggcacgcac ccgatcttcg
     5221 ggcacatcag tgataccggc gctactgacg attttcgccg tcggataaat accgcggact
     5281 aaatcaggcc caccggtacc cacatcctca tccgccgcat tatagagggc caacagcccg
     5341 acccgcagcg cctcttcttc ctggaggccg gatcggtaat gttcacgcat cgtgttgcgg
     5401 gcatccttgc cgccggagcc gatggaatgg tggtcggact cctcgtagcg tccccctgta
     5461 atgtcgtact taaaaatgcg cccttcgttc cgcttgacat cgtatccgac gtaaagcggc
     5521 atgacgacga gtccctgaaa taccatcggg aggttggcct tcaccatctg ccccagcttg
     5581 ttggccttcc cctcacagga cagctgcacg ccttcgagct tctcgtaatg ctcaagctcc
     5641 gtctgaaaca atttggccat ctcgatgcac ggccccgcag ccccggcgat cgccatcgcg
     5701 gaatagtcgt cgatgcgaaa gaccttttca atccgccggt cggcaatctg aaatccctcg
     5761 gtcgcgcgtc gatcaccggc aataatggcc ccatcccgat atttcaacgc gagaaccgtc
     5821 gtcccatgcg gcaccgggat cgttccgggt cggccgaaat ccacgggaag cgcggctcgc
     5881 tggatggcga tctcactcaa accgggtctt aactcaggat gatgcagggt gaggaagtcg
     5941 aagaagctgg acccctcgtg atgtgaaaag a
//
LOCUS       DAORQN010000081         5953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_578_length_5953_cov_11.506104, whole genome shotgun sequence.
ACCESSION   DAORQN010000081 DAORQN010000000
VERSION     DAORQN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_578_length_5953_cov_11.506104"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>321
                     /locus_tag="PK614_05925"
     CDS             <1..>321
                     /locus_tag="PK614_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81784.1"
                     /translation="QNIALGAAITTAVGAVVPGHDIEQVYYLGSFDPQEQVPPALYRI
                     RVRGQASIISWMRFGSGWVPAELIDSLGSSVEINKTNGKIAITKAGGDQLSKLNIGRR
                     LMQFG"
     assembly_gap    322..331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <332..549
                     /locus_tag="PK614_05930"
     CDS             <332..549
                     /locus_tag="PK614_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81785.1"
                     /translation="VIVMGSDPEKFFQAMDEALGAVSQAMVDQENNALHRLLFEALTS
                     TQTEKTLVEAQLEAAQDQFPMAGGKGQ"
     gene            546..4838
                     /locus_tag="PK614_05935"
     CDS             546..4838
                     /locus_tag="PK614_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81786.1"
                     /translation="MNRSITHQGFLGLGLALCSVVSGCAVLTVDVDVYKGSLVNEEHV
                     QLHQLVALATAAKPMLVQLRDNLEWPETDGAPPKGVKLCSNKNKSWYEAGYVKVPEDF
                     VPEPSSASVFQKLSEWFSELINRRAPSCQPHFKNPYARGVNVVLSLYEDLDSPDLAPH
                     GKKLRAALEQLRRVLSDAKGDQAIYDDIVSGFKEDKDLNADLLNLKQGYGELLISSPR
                     EGESSRKVGRLMDALQALANKDSKTQEQEKGEANSQKGLEAELVKEWKGTDFYKKQEE
                     LYDRRLPFRAVWKLLGEGEENTLLAEVSKRLCLESTRGEHACRLLSKRTKELADAYWD
                     SRQAIRELWEESLSLLIRIERLEREVPSRYQTLKEKVITLLVEVTNVRQIASALDRLG
                     GDGKCSVSRNVLALGLMCEVDSDAKMAWSETNVKDNPERFEAILRRALSIAPADIAHF
                     LLNLDSLEKNASPQNANAVSVLVEKSNRINSQRIVRLGLNRSFVDDKKLHGSQEVFQV
                     VDNVSRNLAHGFEHGRLIEGLHTLTENYLNSHDDAKDGADLYDQKRLLDALVEFAQKV
                     LFLANHESLTSPPGTPGLVTGGVESALRGLFGDGIRSAYDKNTLLGIGTANVPEARKQ
                     QYVRVLQAIGNSILFSANEHRERERYRDLSQKKVQAEVAAVNLTHSPDPQKVLDNLLE
                     ELKAEQAVVKQKVSVAKVRKTILEKESLDLSATRLPDLSAKQATADAAATTAQANLAT
                     FQSATKALKGFHQVLITGGLVNKIPQAWSTPVPSVSVVECFLKIAMAGGNSCAGQWTG
                     LATGGSADSIEQGINREKTINWPTLTKEQQETYKEAIAYITAQETLQSVMDYRQREGR
                     RSLKADDLLKDLTDHIRLLEQKRVADGQNLVAQVDQARKVQTDYQTDIASANNRVEVL
                     KSELSTIEVLVAAHPFDSQQIENAIQEIGKLSGSVRKYADASGKYLSPKGVYEHLENL
                     LKQLKQLKQQVATASASQGTSLQDALDVLSKRRPPTEMPLLDPKIYANPREVMDELIA
                     LLRHRQIEAVERFGKGSEQDKRATEAVENAHQHRAGMIYIRPSSAYLRTSFPSTSLQD
                     DPNLAWDNMLLKQGIRNLPFSSQLADILDPSGRQDRLLTSELDKQYWQNVNRVRVSGM
                     GFTNQVLAKDDVGNWYVKQYYGDTKDIIKSAKNLALFNLGTKLPIDLAGELKAASKLK
                     GDTKTEAAGESPPLNRVLEKHRNVYQAKTTEASTKLEELHAKNGKNTVHDQIVKGWEN
                     VEEMKSDTDSMDALKAALDVEIREWDKAAEALKSKPDQDRGQLIVKDIRALSKFEKLL
                     SVKIQEIDKEAESKPQKKRKGLGKILDEKIVDLAATPDDSNKKKAVDEAQKAIADEIK
                     RLSTLKTKAMTEVGRVVGPLVLDMLKDRKRALDSYEQAIMFIGDAANSNSSP"
     gene            complement(5162..>5953)
                     /locus_tag="PK614_05940"
     CDS             complement(5162..>5953)
                     /locus_tag="PK614_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81787.1"
                     /translation="DQEIATLKTWVEQLAPKDAEIARLRTRTEELEPFLEKSRRLERE
                     QEQDRARASAILQAKEQQLAQTQARLNAIELDLANLRTQSQEDLARFGETLQERDAEI
                     QRLRATVEELEMFRGEVEKKDLALREAEERRVMDVSEREEEIGALRKRLVEYRVAQRF
                     SAQAKSTGATESGSTGSATTKRGAGKGHPAQKDDLKQIHGIGPVMERVLNRMGMFTFR
                     QIAEWKEQDVEHMASELNTFPDRIRHDNWIDGAKEQHFLKYGEEL"
BASE COUNT         1533 a         1354 c         1690 g         1366 t
ORIGIN      
        1 tcaaaatatt gcattgggcg cggcgatcac gactgcggtc ggggccgtcg tgcccggtca
       61 cgacatcgaa caggtgtact acctcgggtc gtttgatccg caagagcaag tcccccccgc
      121 gctgtaccgc atccgtgtgc gtggacaggc cagcatcatc agctggatgc ggttcggatc
      181 cgggtgggtc ccggctgaat tgatcgactc gcttggaagt tcggtagaga tcaataagac
      241 caatggcaag atagcaatta ccaaggccgg cggcgatcag ctctcaaagt tgaatatcgg
      301 acgccggctc atgcaattcg gnnnnnnnnn ntggtcatcg tgatgggttc cgacccagag
      361 aagttttttc aagccatgga cgaagccttg ggggccgtga gccaagccat ggttgatcaa
      421 gagaacaatg cgctccatcg cttgttgttt gaagccctga cgagtacaca aactgaaaag
      481 acgttggtcg aggcacagtt ggaggcggcg caggatcaat ttccaatggc tggagggaaa
      541 gggcaatgaa tcgctctatc actcaccagg gattcttggg actggggttg gcactatgta
      601 gcgtagtcag cggctgcgcg gtcttgacgg tggatgtgga tgtctacaag ggctctctgg
      661 tgaatgagga acatgtacaa ctgcatcaat tggtggcttt ggcgacggcg gccaagccga
      721 tgttggttca actaagagac aatttggagt ggcctgagac tgacggcgcg cctccgaaag
      781 gcgtcaaatt atgttcaaac aaaaacaaga gctggtacga ggctggttat gtaaaagtgc
      841 cagaagattt tgtgccggag ccttcatctg catcggtgtt tcagaaattg tcagagtggt
      901 tttcagaatt gattaatcgt cgtgcacctt cgtgccaacc acacttcaag aatccctatg
      961 ctcgtggagt gaatgtcgtc ctgagtttgt atgaagactt agattctcca gatctagctc
     1021 cgcatggcaa gaaattacgg gcggccctgg agcaattgcg gcgagttctg tcggatgcga
     1081 agggtgatca agcgatttat gacgatattg tatcaggatt caaggaggac aaagacctca
     1141 atgctgacct cctgaatcta aagcaaggct atggtgaact cctcatttca tcccctcgag
     1201 agggagagag cagcagaaaa gtcggaagat tgatggatgc actgcaggcg cttgcaaaca
     1261 aagatagtaa aacgcaagag caagagaagg gagaggctaa tagtcagaaa ggattggaag
     1321 ctgaactggt aaaggagtgg aaaggaacag atttctataa gaaacaagaa gaactttatg
     1381 atcggcggtt accgtttcga gctgtgtgga aacttctcgg cgagggagaa gagaatacgc
     1441 ttctggccga ggtaagcaag cgattgtgcc tggaaagtac tcggggcgag catgcctgcc
     1501 gtctgctcag taagcgcacc aaggaactgg ccgatgccta ttgggattcg agacaagcca
     1561 ttcgtgagtt atgggaagaa agcttgagtt tgctgatacg gattgaacga ttggagaggg
     1621 aagtcccaag ccggtaccag accctaaaag aaaaggttat cacgttgctg gtcgaagtta
     1681 ccaatgtgcg tcaaatcgca tcggctttgg atcgtttagg aggcgatggt aagtgttctg
     1741 tatcaaggaa tgtcttagcg ctcggcttaa tgtgcgaagt cgactcggat gcgaagatgg
     1801 catggagtga aactaacgtg aaggacaatc cagagcgctt tgaggcgatc ttgcgacggg
     1861 cgttgtctat cgcaccggca gacattgcgc atttcctctt aaatctcgat agcttggaaa
     1921 aaaatgcttc tccgcagaat gctaatgctg tgagtgtgct tgtcgaaaaa tccaatagga
     1981 tcaattcgca acggatagtt aggctggggc taaaccgcag cttcgtcgat gacaagaagt
     2041 tgcatggctc acaagaagta tttcaggttg tggacaatgt gagtcgcaac ttggctcacg
     2101 ggtttgaaca tggtcgattg attgaggggt tgcacacgtt aacggagaat tatctgaact
     2161 cgcacgatga tgcaaaagac ggcgctgacc tatatgatca aaagaggctc cttgatgcgt
     2221 tggtggaatt cgctcaaaag gtgcttttct tggccaatca tgaaagcttg acgtcgccac
     2281 cagggacccc aggacttgtt acgggcggtg ttgaaagtgc cctgcgtgga ttgtttggcg
     2341 atggaattag atctgcttac gataaaaaca ctcttctagg tatagggaca gctaatgtgc
     2401 ctgaagccag gaagcagcag tatgttcgtg tcttacaggc tatagggaac tccattctgt
     2461 tttcggcgaa tgaacatcgc gaacgggagc gttatcgtga tttgagccaa aaaaaggtac
     2521 aggccgaagt ggccgcggtg aatttgacac actcgcctga tcctcaaaag gtgttggata
     2581 atttgctgga agaattgaaa gcggaacagg ctgttgttaa acaaaaggtg tcggttgcaa
     2641 aggttcgcaa gacgatctta gagaaagaat cgttggattt gagtgctacg cgactgcctg
     2701 atttgagtgc aaaacaggca actgctgatg ctgcagccac aacggcacaa gccaatctcg
     2761 ccacttttca atcagcaaca aaagccctga aaggtttcca tcaagtgttg atcactggag
     2821 ggctggtcaa caagattccc caagcctgga gtacgccggt tccgagtgtg tccgtcgttg
     2881 agtgttttct taagattgct atggcaggcg ggaattcttg cgctggacaa tggactggtc
     2941 tggcgactgg tgggagtgcg gatagtattg agcaaggtat aaatagggaa aaaaccatta
     3001 attggccgac cctgaccaaa gaacagcagg aaacgtacaa ggaggcaatc gcctatataa
     3061 cagcgcagga aactcttcag tcggttatgg attaccgaca gcgagaaggg cgcaggtcgt
     3121 tgaaggcgga tgatttactg aaagatctta ccgatcatat tcgtttactt gaacagaagc
     3181 gtgtagccga tggacaaaat cttgtcgcac aagttgatca agctcggaaa gtacaaactg
     3241 actatcagac ggacattgcc agcgcgaata accgggtcga agtcttaaaa agtgaactct
     3301 cgaccatcga agtcctcgtt gccgctcatc cttttgactc ccaacagatc gagaatgcga
     3361 tacaggaaat tggcaaactc tcgggctctg tgcggaagta tgcggatgcc agtgggaagt
     3421 acctttctcc caaaggagtc tatgagcatc ttgaaaatct attgaaacaa ctgaaacaac
     3481 tgaaacaaca agttgccacg gcaagtgcta gtcaaggtac atcgctccaa gatgcacttg
     3541 atgtgttatc gaagaggcgc ccgccaaccg aaatgcctct ccttgatccg aagatctatg
     3601 ccaatccgcg agaagtcatg gacgagctga tagcattgct tcggcaccgt caaatcgaag
     3661 cggtggagcg tttcggcaag ggctccgagc aggataaacg ggcgacggaa gctgtagaga
     3721 atgcccatca gcatcgagcg ggcatgatct atatcaggcc ttcatcggcc tacttgcgga
     3781 ccagcttccc ctctacttct ttgcaagacg atcccaattt ggcttgggac aatatgttgc
     3841 tcaagcaggg gatcaggaac ctgccgtttt cctcccaact cgcggatatt ctcgatccct
     3901 caggaaggca agatcggtta ctgacgtccg aattggataa acaatactgg caaaacgtca
     3961 atcgcgtacg ggtgtccggg atggggttta cgaatcaggt gctggccaaa gatgatgtgg
     4021 ggaattggta tgtgaaacag tattatggcg acacgaaaga cattatcaaa tcggcgaaaa
     4081 atcttgcgtt gtttaatttg ggcacgaagt tgccgatcga tctggcaggt gaactgaaag
     4141 cagcctcgaa actgaaaggc gataccaaga ccgaggctgc aggggagtcg cctcccctaa
     4201 accgggtgtt agagaaacac cggaatgtgt atcaggccaa aaccactgaa gcttcaacga
     4261 agctcgaaga gctacacgca aaaaacggaa aaaatacggt tcacgatcag atcgtcaaag
     4321 gttgggaaaa tgtcgaagag atgaaaagcg ataccgattc gatggatgcg ttaaaagctg
     4381 ccctggacgt tgagattcgg gagtgggata aagcggccga agcactcaag agtaaacccg
     4441 accaggatcg tggtcagtta attgtgaaag atattcgggc tctttccaag ttcgagaaat
     4501 tactttcggt aaaaattcag gagatcgaca aagaggcgga gtcgaagcct caaaagaagc
     4561 ggaaagggct tggaaagatc ttggacgaaa aaatagtgga tcttgccgct accccggatg
     4621 atagcaataa gaaaaaagca gtggatgagg ctcaaaaagc catagccgat gagataaaga
     4681 ggctttcgac cttaaaaacg aaagcaatga ccgaggtggg tagggttgtg ggtcctctcg
     4741 tgctggacat gctgaaagac cgaaagcgag cattggacag ttacgagcag gcgatcatgt
     4801 tcataggaga cgctgccaac tctaatagtt cgccttaaga agaagttcgc catctcctga
     4861 gattcggccg ccgattcatg gcaaaattgg aatgcggcag tcactctttc caaaagtctg
     4921 acggcgaggg gagggttctc gtatgaagtg gcggcggttg ggacgatggt ctaaagaatc
     4981 cgcgggacgc tcacaacgcc cgcaggatgt tcaaaatggc cgtccccttc gtccataaag
     5041 cgtgtgcagc tgcaatgctc aatgttcaag tgagcattga acctttccgg gaaaagcgag
     5101 atccgattca cggatgggta agcgatggag ggtgaggccg gcggacggtt tcgagatccg
     5161 gctacagttc ttcgccgtat ttcaggaaat gctgctcctt ggcgccgtcg atccagttgt
     5221 catggcgaat gcgatcgggg aaggtgttga gctccgatgc catgtgttcg acgtcttgct
     5281 ccttccactc ggcgatttgc cggaacgtga acatccccat gcgattgagg acgcgctcca
     5341 tcaccgggcc gatcccgtgg atttgtttga ggtcgtcttt ttgggccggg tgaccttttc
     5401 ctgcaccccg ttttgtggtg gcgctaccgg ttgatcccga ctcggtggct cccgtcgact
     5461 tggcttgcgc gctgaaccgc tgggctacac gatactcgac gagtcgtttg cggagcgctc
     5521 cgatctcttc ttcccgctcg ctcacatcca tcacacgacg ctcttcggcc tcgcggagcg
     5581 caaggtcttt tttctccacc tcgccccgga acatctccaa ctcttcgact gttgcccgta
     5641 gacgctgaat ctccgcgtcc cgctcctgca atgtttcgcc gaatcgcgca aggtcttcct
     5701 ggctctgtgt tcgcaagttg gcgaggtcca gctcgattgc gttgaggcgt gcctgggtct
     5761 gtgcgagttg ctgttctttg gcctgtagta tggctgacgc gcgggcgcga tcctgctcct
     5821 gttcgcgctc caatcggcgg cttttttcca ggaacggttc caactcttcc gtacgggtgc
     5881 gcagccgggc gatctcggca tctttcggtg ccagttgctc cacccaggtt tttaaggtag
     5941 caatctcctg gtc
//
LOCUS       DAORQN010000082         5849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_585_length_5849_cov_8.373145, whole genome shotgun sequence.
ACCESSION   DAORQN010000082 DAORQN010000000
VERSION     DAORQN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_5849_cov_8.373145"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..669)
                     /locus_tag="PK614_05945"
     CDS             complement(229..669)
                     /locus_tag="PK614_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB81788.1"
                     /translation="MEGYGKRVLIAEDEESVRRLLAVVLEEAGYTVHAVEDGMEALAE
                     MKKRRFDAVVADYRMPRLDGEQFLLLSRLMWPQTPTVLLSAEHTDLPVILKMQGACAL
                     VPKPFDSQGLLQVLQSAVEAACSCCSPSSIPREGTNNTLSVAPS"
     gene            complement(1053..1310)
                     /locus_tag="PK614_05950"
     CDS             complement(1053..1310)
                     /locus_tag="PK614_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81789.1"
                     /translation="MDPQVVSPGDGAIDEQILGLLQHHRRLTFLTLAESLPLYTWQAL
                     FAALNRLRGQCGPATAGRRLRSGLAAGERTPVPDVRRALKM"
     gene            1699..2124
                     /locus_tag="PK614_05955"
     CDS             1699..2124
                     /locus_tag="PK614_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB81790.1"
                     /translation="MSRVGIPADGFKTVGQIVGTNTIQFHTAQDGMAITIELLSAHVS
                     GGPVLDAEGRYVGFISEFDILKALEAGKDLNSLTATELMARDPIAVRKSTPIPDAIKL
                     MADNHLLNLPVEENGEVTYSVTRHDLLRASVGLAVGIEE"
     gene            2152..2514
                     /locus_tag="PK614_05960"
     CDS             2152..2514
                     /locus_tag="PK614_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRB81791.1"
                     /translation="MIRIKRVYETPSARDGIRILVDRIWPRGCTKEQARLDAWRKEVA
                     PSTALRTWFGHDPAKWTEFQTRYRKELSRPEQNRAIEDLARLAQAQTVTLLYGASDKE
                     HNQAVVLKECIDSAKNRV"
     gene            complement(2842..3498)
                     /locus_tag="PK614_05965"
     CDS             complement(2842..3498)
                     /locus_tag="PK614_05965"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRB81792.1"
                     /translation="MNIRGRFIGFLIVAIAALAAVFFGHLFLFEQWRVQQERQLHRSK
                     ILGNVAHLQRLVLEVETNFRGYLLTEQQSFLEPIRLAENRLKTGLAHLTDLTANTPGL
                     QAGVSVLSARLDEFVESKQRLLAVIGAEQQDLVRSYVRGGSGRALFLTIEKAIGDFEV
                     RIERELPSEPMTYDSWLQRARWQLLLVESLGVIVCVFLTRAALLPNRTVLAPNPRPLL
                     "
     gene            complement(3745..5034)
                     /locus_tag="PK614_05970"
     CDS             complement(3745..5034)
                     /locus_tag="PK614_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit beta"
                     /protein_id="HRB81793.1"
                     /translation="MPEVYNWQLGRKMLYPYEERHPKWQFAFVFNINRCLACQTCSMA
                     DKSTWLFSKGQEYMWWNNVETKPYGGYPQFYDVKITQLIEQVNPGGQVWNVRVGRKHH
                     APYGVFEGMTIFDAGAKVGQAAIGYIPTDQEWRFVNIYEDTATSMRALVENIDKSGFT
                     RDEPWRLSGSSLPEHETYFFYLQRICNHCTYPGCLAACPRKAIYKRPEDGIVLIDQNR
                     CRGYKKCVEQCPFKKPMYRGTTRVSEKCIACYPRIEGKDPLTGGEPMETRCMAACVGK
                     IRMQSLMRIGEDGLWAEDRWHPLYYTIRVEQVALPLYPQWGTEPNGYYIPPRHSPRGY
                     ARQMFGPGVDNAIEKYLVPSRELLAVLQLWRASQQIVFRYDVIPGPKVFETQIHGKRF
                     DMYNDTVLGFNKSGKEVARIQVEEPIYIRPAERVNWL"
     gene            complement(5143..>5849)
                     /locus_tag="PK614_05975"
     CDS             complement(5143..>5849)
                     /locus_tag="PK614_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit alpha"
                     /protein_id="HRB81794.1"
                     /translation="PKTRHRVHSQWSVNDWVQIYESNFGDPYRMDKRTPGVGEHQIHI
                     NPQAAKDRGINDGDYCYVDGNPVDRPYRGWKPSDPFYKVARLMIRAKYNPSYPYHVTM
                     AKHAPYVSTAKSVKGHETRPDGRAIAVDTGYQSNFRYGAQQSFTRSWLMPMHQTDSLP
                     GKQANALKFKWGFEIDHHAVNTVPKECLIRITKAEDGGIGARGPWEPVRTGFTPGQEN
                     EFMIKWLKGEHIKIKV"
BASE COUNT         1221 a         1865 c         1563 g         1200 t
ORIGIN      
        1 tccgtttcgc cggacgcgag gcggccttct tcggcgccgt ctttttcttc atgcattctc
       61 catctacact tgtatctagt catcagcccc gcctctcacc atacttttcg cgagagatct
      121 gttcctacga taccatattc aggccggatc tgatcaggcg gcagaacgtg cccctcaacc
      181 ccgtctccct ccttcaagca cagacacctt cctcgaggtg accacccgct aggaaggcgc
      241 tactgagagg gtgttattcg tcccctcacg cgggatcgaa ctcggagagc aacaactaca
      301 ggccgcctct accgccgact gcagaacctg caggagcccc tgcgaatcga agggtttcgg
      361 cacgagtgca caggcgcctt gcattttcag gatcacgggc aaatccgtat gctcggcgga
      421 caggagcacg gtcggagtct ggggccacat caggcgactc agcagcagga actgctcccc
      481 atccaggcgg ggcatgcggt aatccgcgac caccgcatcg aaacgccgct tcttcatttc
      541 ggccagggct tccatgccat cttccaccgc atgcacggta tacccggctt cctcgagcac
      601 cacggccagc aggcgccgta cactctcctc gtcctccgca atcaacacac gttttccata
      661 cccttccatg gcacctcctc cctccggctg cattccaccg acggaacacc ctcattcctt
      721 ccgtcacgac cataatcccg tccgctcaaa cggtgtagct actctggttc cggtttccat
      781 aaccgacgaa tccatatgcc tctgcgttgc ggaccagggc gaacccttcg atgaagttgt
      841 tccggaaggc gtcgtgccag gcggattaac ggatcttcca cattgccgca attgaggcaa
      901 cgcgtgactg acacccacat atgcgacact ccttcgctgg gactaaacag gttttcggcc
      961 accatcagac cccgacatcg actgcaggac atcctttgcc tccttcgatc actcgcaccc
     1021 gccgcgcggt cgtgcccatc gcaacatcgc catcacatct tgagggccct tcggacatca
     1081 gggacgggcg ttcgctcccc tgctgccaga ccacttcgta atctgcggcc agcggtagca
     1141 ggtccacatt ggccgcggag ccggttcaac gccgcaaaca atgcctgcca ggtgtacaat
     1201 ggaagggatt cggccagggt gaggaaggtc agccgccggt ggtgctgcag caaaccgaga
     1261 atctgctcgt cgatggcgcc atcgcccgga gaaacaacct gagggtccat cacgcacctc
     1321 cattgtggac agagcggact actcgcttca cgtgtggtcc agcccatccc cggagacgtt
     1381 gcaatatgtg atcctgctga cggcacacat ccagcgaacg gaatccgcca ttcagcaatg
     1441 gttacggcat gctcactccg caacagctgt ccttaaccgg gacattccgc actgtctgcc
     1501 ccgtgcggaa tcgctcgcac ccataggatc atcgcctgta acggcgcagg aattcaggaa
     1561 atcgcaaccc atccggacgg gcacttcgct tgctgagctg atgagcaaga gttcgcagga
     1621 acagcgcagg ccgcagttca tcgacctgcc acacatctcg caccctcgat ggctgttgcc
     1681 gcatcgaaag gagacatcat gagtcgcgta ggaattccag ctgacggttt caaaaccgtc
     1741 ggacaaatcg tcggaaccaa tacgatacaa ttccataccg cacaggatgg catggcgatc
     1801 accatcgagt tgctttccgc gcatgtgtcc ggcggaccgg tgttggatgc agagggccgc
     1861 tatgtgggat tcatcagtga atttgatatc ctcaaggccc ttgaggcagg aaaggacttg
     1921 aacagtctga cggcgacaga gctgatggcc agggatccca ttgccgtccg taaatcgaca
     1981 ccgataccgg acgcgatcaa gctcatggca gacaaccact tgctgaacct ccctgtcgag
     2041 gagaacggag aggtgaccta ttcggtgacc aggcacgacc tgctccgtgc atcagtcggt
     2101 cttgccgtcg gcatcgaaga gtagccgcgc gcgacttgtt gctgtgcaat catgattcga
     2161 atcaaacggg tctacgaaac accaagcgcg cgcgatggga ttcggattct ggtcgaccgg
     2221 atatggccga ggggatgcac gaaggagcag gcgcgcctcg atgcatggcg aaaggaagtg
     2281 gctcccagca cggcgctccg cacttggttc ggccatgacc cggcaaagtg gacggagttt
     2341 cagacacgtt accgcaagga actgagccga ccggaacaga accgcgcgat cgaagatctc
     2401 gcccggctcg cacaagcaca gaccgtcaca ctgctctatg gggcatcaga caaggaacat
     2461 aatcaggccg tggtcctgaa ggaatgcatc gacagtgcga agaatcgcgt ttagctcgcc
     2521 aacccccagg tgctccagag agcgaccgcg gcaatcgcca acatcaccca ggccaactct
     2581 cgatctccga ctttcttgat catccgtacc atgggctgac tccttgtttc atatgcgttc
     2641 actgtgatga acgtctttaa agcaaggcac atgccattac tcatgaacgg aattccaatg
     2701 gttttcagat tttcccagcc tcaaatcgag cgaattcgca cgctacgcac tgtgcgaata
     2761 gacacatgga gagcgacatg ggggtagatc gcgacaggta actgaatcga tgggaggaac
     2821 aactcgatag cccggataga ctcacagaag aggtcgaggg ttcggtgcca gcacggtcct
     2881 attcggaaga agcgccgctc tcgtcaggaa cacacacact atgaccccca gactttccac
     2941 caacaacagc tgccagcgtg ctcgttgcag ccacgagtca taggtcatgg gttcactcgg
     3001 cagttcgcgc tcaatgcgca cttcaaaatc cccgatggct ttttcgatgg tcaggaacaa
     3061 cgcccgacca ctccctcccc gcacatacga gcggacgagg tcctgctgct ccgccccgat
     3121 gaccgccagc aatctctgtt tgctctccac gaattcatca agccgggccg acagcacact
     3181 cactccggcc tgcaaacccg gagtgttggc ggtcaaatcg gtcaagtgag ccagtcctgt
     3241 tttcagtcga ttctccgcca gccgaatcgg ttcgagaaac gactgctgct ccgtgagcag
     3301 atagccgcga aagttcgttt ccacctccaa gaccaaccgt tgcaaatgcg ctacgttacc
     3361 gagaattttg gaacggtgga gctgccgctc ctgctgtacc cgccattgtt caaacaggaa
     3421 caggtggccg aagaacaccg cggccagcgc agctatggcg acgatgagga accctatgaa
     3481 ccgtccccgg atattcatca ctctctgtca cgcattccaa gcctcagaac atcagcggat
     3541 cgaaacgtga tgcgcccctt tggccttcgc ccggacttcg cccacgctgc atggcctcat
     3601 cggccagtaa caggcggggg cagtccccgt gaaggatcgc tccctcacga agactacccg
     3661 caggccttcc tctcacagga tcggcggcac aggcccacgc aacgccatcg ttgcatccgc
     3721 gcgcgacgtt gcgcggccct cgacttacag ccagttcacg cgctcggccg gccgaatgta
     3781 gatcggctct tcgacctgaa tacgcgccac ttccttgccc gacttgttga agcccaacac
     3841 ggtatcgttg tacatgtcga accgcttccc gtggatctgg gtttcaaaca ctttcggacc
     3901 cggaatgaca tcgtaccgga agacgatctg ctggctggct ctccagagct ggaggacggc
     3961 caacagttcg cggctgggca cgaggtactt ctcgatggcg ttatccacgc ccgggccaaa
     4021 catctgccgc gcgtagcccc gggggctatg ccgtggcgga atgtagtagc cgttgggctc
     4081 ggtgccccac tgcggataca gcgggagcgc cacctgctcc acacgaatcg tgtagtacag
     4141 gggatgccag cggtcttccg cccacaggcc gtcttccccg atgcgcatca acgactgcat
     4201 gcggattttg cccacgcagg ccgccataca acgcgtttcc atcggttcgc cgcccgtcag
     4261 cgggtctttg ccttcgatgc gcggataaca cgcgatacac ttttccgaga cgcgcgtcgt
     4321 gccccgatac atcggcttct tgaacgggca ctgttccacg cacttcttgt acccgcggca
     4381 ccggttctgg tcgatcaaca cgatgccgtc ttccggccgc ttgtagatcg ccttgcgcgg
     4441 gcaggctgcc aggcagcccg gataggtgca gtggttgcag atccgctgca gatagaagaa
     4501 atacgtctca tgctccggca agctgctgcc ggagagacgc cacggttcgt cgcgcgtaaa
     4561 gcccgacttg tcgatgttct cgaccagggc ccgcatcgag gtcgccgtgt cttcatagat
     4621 gttcacgaac cgccattcct ggtccgtggg gatgtacccg atcgccgcct gcccgacttt
     4681 ggcgccggcg tcgaaaatgg tcattccctc gaacaccccg tacggcgcat ggtgcttgcg
     4741 tcccacgcgg acgttccaca cctgcccgcc cgggttcacc tgctcgatga gctgggtgat
     4801 cttcacgtcg tagaactgcg gatacccgcc gtacggcttg gtttccacgt tgttccacca
     4861 catgtactcc tgccccttcg agaagagcca ggtcgacttg tcggccatcg aacaggtctg
     4921 acaagccaaa cagcgattga tgttgaacac aaaggcaaac tgccacttcg gatgccgctc
     4981 ctcgtaggga tacagcatct tccgtcccag ttgccagtta tagacttctg gcattgtgaa
     5041 tcctccttct gaacatgatc atgtagttaa tggttcgcat tcgtttcaag caccgaggac
     5101 gaagtgccga gaggcgtgcc tctcagccta tgtacctctg tcctagacct tgatcttgat
     5161 atgttcaccc ttaagccact tgatcatgaa ctcgttttcc tgacccggcg tgaacccggt
     5221 ccggactggt tcccacggac cacgcgctcc gataccgccg tcttccgcct tggtgatgcg
     5281 gatcaaacac tccttcggca ccgtattgac cgcgtggtga tcgatttcga atccccactt
     5341 gaacttcaag gcattcgcct gcttgcccgg cagggagtcg gtttggtgca tcggcatgag
     5401 ccagctcctg gtgaatgatt gttgagcgcc gtaccggaag ttggactgat agccggtgtc
     5461 caccgcaatg gcgcgtccgt ccggccgcgt ctcatgcccc ttcaccgact tggccgtaga
     5521 cacataggga gcgtgtttcg ccatcgtcac atgatacggg tatgaaggat tgtatttcgc
     5581 ccggatcatc aaacgcgcga ccttgtaaaa cggatccgac ggcttccagc cgcgataggg
     5641 ccggtccacg ggattgccgt ccacataaca atagtcaccg tcgttgatcc cgcgatcctt
     5701 cgccgcctgc gggttgatgt ggatctggtg ctcgccgaca cccggtgtcc gtttatccat
     5761 gcggtacgga tcgccgaagt tcgactcgta aatttgcacc cagtcgttca ccgaccattg
     5821 gctatgcacc cggtgccggg tcttgggcg
//
LOCUS       DAORQN010000083         5804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_587_length_5804_cov_8.106279, whole genome shotgun sequence.
ACCESSION   DAORQN010000083 DAORQN010000000
VERSION     DAORQN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_587_length_5804_cov_8.106279"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..680
                     /locus_tag="PK614_05980"
     CDS             105..680
                     /locus_tag="PK614_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRB81795.1"
                     /translation="MRKLIAGMKISVDGKIEGPEGHADWVEAWSDDYGLEPQVDACVL
                     GGGMYPGYEQYWTAIQNTPDKPLPMTGKLPTRGEVEWARFAAQTPHYVLSCTLASALW
                     PKASFIRKLEEVAALKQQPGKDIYLVGGARTTASLIDAGLVDELRLIVYPLIAGGGKA
                     LFATTERRRGLQLQKVQQLQGGRVSLIYGIG"
     gene            708..1121
                     /gene="tsaA"
                     /locus_tag="PK614_05985"
     CDS             708..1121
                     /gene="tsaA"
                     /locus_tag="PK614_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRB81796.1"
                     /translation="MTSFEVRAIGWVSSDLKDLDLAPRQADEGAPEAWSVFEPEMSEG
                     LQNLKSGDHVLVLTWLNRARRDILRVHPRGDTSRPLEGVFNTRSPHRPNPIGLHPVEI
                     TATEGLRLRVRHLEALDGTPIIDVKPILDAEVSRR"
     gene            1214..1621
                     /locus_tag="PK614_05990"
     CDS             1214..1621
                     /locus_tag="PK614_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB81797.1"
                     /translation="MISKIFVNLPVKDLDKSMAFFKAVGFSFNPQFTDKTAACMVMSD
                     DIYAMLLTHDKVKEFTEKPIADARKTTELLTALAVENKAKVNELADKAIKAGGTEAYA
                     PKDYGFMFARSFEDPDGHIWEVFWMDPTQVQKG"
     gene            2069..2659
                     /locus_tag="PK614_05995"
     CDS             2069..2659
                     /locus_tag="PK614_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HRB81798.1"
                     /translation="MRIITVAIISLLTVSPVLAKEKTHDKPMDSQAMMELWQKLAQPG
                     EPHKTFAGLAGSWTTTTKEWMEPGKPPTEASGTAEMKMLLDGRFLYQEFKSQMMGQPF
                     SGIGIDAYDNVRKKYVTAWMDTMGTGIFIMEGTASADGKTITLKGSHPEPGGGQMHHR
                     AVWKLVDSNTQTFEMYGNHGHGKEAKMMEITYTRKP"
     gene            2791..3225
                     /locus_tag="PK614_06000"
     CDS             2791..3225
                     /locus_tag="PK614_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81799.1"
                     /translation="MQRTIGFLAMALILTSLSGCSYLFYPRAGDYATQAKGANGVETM
                     MNLSSMMEATASKAKGGKGVDTAFDDLHNQFHALSDSFCGVTDAQSKTPAYALAVTHK
                     KELGAIFKRLWKFKDDQPQRDQHLELLATELKELRETLQTIK"
     gene            3674..4870
                     /locus_tag="PK614_06005"
     CDS             3674..4870
                     /locus_tag="PK614_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB81800.1"
                     /translation="MARRSRFSTVAPMIYGCLALALLSSCKQEVGSSQAPPVPEVGIV
                     VATAQDVPDEPEFIGQSESSRPVEIRSQVTGILKEWFFKEGRDVKKGDRLYQIDPVPF
                     HAAMLSAKAKVAQAEARLVQAKQNLARVKPLLAEQAVSTKDVDDAVAEEMAAKANLEG
                     AKAELVKAKFDLDNTLIVAPISGMIERTRVYEGRLVSAQTDLLTIIQQVDPMYVIVSA
                     PESFLLKRQRDSTAKRIQHPGVYQLRGVLTFADGTTYGQEGVLDLLDVGLKTDTGSRQ
                     ARVVFPNHDRVLLPGQFVRVRFKGTVKTGAILVPQRAVQQGAKGSIVFVVGNEDKVEM
                     REVQATSWQGNQWIVEQGLHVGDRIIVEGLHKIAPGAPVKPVPLPAPAATTVPTAAKP
                     QPERAS"
     gene            4867..>5804
                     /locus_tag="PK614_06010"
     CDS             4867..>5804
                     /locus_tag="PK614_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB81801.1"
                     /translation="MISHFFIDRPIFASVLSIVIMVVGLVALKALPVAQFPEITPPVV
                     QIEADYPGANAEIVADSVARPIEVQLPGIDNLLYYDSTSTNDGHMSIKLTFEIGTDVD
                     IAQVQTQNRVKLAEPQLPPEVVRQGISINKVSPDLLAVVALSSIDPTHDTVYLSNYAI
                     LRVLDNVKRLRGVGNAVVFGSQNYSMRLILDPIRMAQLSLTPTDIANVVREQNRDFPA
                     GTIGREPALKGTELTIPVITQGRLTEVKEFEELIVRAMPNGSMIHLKDVARVELGAQS
                     YTLEGRWNGKPNVFLLTFLSPGANALETVKRVRAELA"
BASE COUNT         1349 a         1528 c         1778 g         1149 t
ORIGIN      
        1 gtctcaagca actcattgaa actgggagcg gtaaacctgc tccgcgagat atcaaaatcg
       61 ataactggaa ttaactcgca tgagattctg tagcggagga tgacatgcgg aagttgattg
      121 ctggaatgaa aatttcagtg gacgggaaga tcgaggggcc ggagggacac gcggattggg
      181 tcgaagcgtg gtcggatgac tacggcctgg agccacaagt ggacgcgtgt gtgcttggag
      241 gaggcatgta tcccggctac gagcagtatt ggacagcgat ccaaaatacg ccggataagc
      301 cccttccgat gactggcaag ttgccaacgc ggggtgaggt tgaatgggct cgtttcgccg
      361 cacagacccc acattatgtg ctgtcgtgca ccctggcttc agctctctgg cctaaagcga
      421 gcttcatccg gaaactcgaa gaagttgcag ccctcaagca acagcctggc aaagacatct
      481 atcttgttgg gggtgctcgt acgactgcga gcttgatcga cgccgggctg gtggatgaac
      541 tccggcttat tgtgtatccg ctgatcgccg gcggagggaa ggcgcttttt gcaacgacgg
      601 agcgtcgtcg tggacttcaa ctacagaaag ttcaacagct tcaaggtggg cgtgtcagcc
      661 tgatctacgg aatcggctaa gggagcaggc tgggctcagg aacggatatg acaagtttcg
      721 aagtgagagc aatagggtgg gtctcgtcag atctgaaaga tcttgacttg gcccctcggc
      781 aggcagacga aggcgcaccc gaagcctggt cggtctttga gccggagatg tcggagggtc
      841 tccaaaacct gaagtccggt gatcacgttc ttgtcctcac ctggctcaat cgggcgcgcc
      901 gggacatttt acgcgtgcat ccgaggggag atacctctcg accgctggag ggcgtcttca
      961 acacacgctc tcctcaccgt ccgaatccta ttggcctgca ccctgtagaa atcaccgcga
     1021 ctgaaggcct gcgattgcga gtgcgccatc tcgaagcgct ggatggaacg ccgatcatcg
     1081 acgtgaaacc catccttgat gcagaagtca gtcgacgtta agtagtgggc atgcaccttg
     1141 gagaaacaat caaaatgatc cgaacgctaa atcataactt tctcgaatca cccaatagca
     1201 aaaagagcca gaaatgatca gtaaaatatt cgtcaatctc cccgtgaaag acctcgacaa
     1261 gtcgatggcg ttcttcaagg cggtcggctt ctcgttcaat ccgcagttca cggataagac
     1321 cgcggcttgc atggtcatga gtgacgatat ctacgccatg ttgttgaccc acgataaggt
     1381 gaaggaattt acggaaaagc cgattgcgga cgcccgcaag acaactgaac tgctgaccgc
     1441 ccttgctgtc gagaacaaag cgaaggtgaa tgagcttgcg gacaaagcga ttaaggccgg
     1501 agggacggag gcctatgctc ccaaggatta cgggttcatg ttcgcgcgca gcttcgaaga
     1561 tccggacggc catatctggg aagtcttctg gatggatccc actcaggtgc agaagggata
     1621 gaggctctcc ctgtgcgtga cgaacgatcc ctcgtggagc agattcgatg aataggaaac
     1681 gaaccatacc cgaaaccgta caggacgaca tcgccggctt cccacctgcc gttcgaacca
     1741 acctcaagaa aatcggcaga cgatcaggag agccgcatct ggtgcgaagg aggcgatgag
     1801 ctatggaatg ccaacgttga cgctgaacgg cgcattgatc tacttcgccg gtttcaaatc
     1861 gcacattggg ctgtatccaa tgacggcgcg cgttcgaaag cactttaaga acgaactcga
     1921 ggatacctca gtggcaaagc gaccgcacga ttcccgctcg acaagcccat ttcttatccc
     1981 ttgatcgagc gagtcgtgaa ggttagggtg aaagagcacg tagtaagagc agacggcacg
     2041 aagaattggc aaccacaagg aggcgactat gcgtattata actgttgcga tcatctcgtt
     2101 gttgaccgtc tcaccagtgc tggcgaagga gaagacgcac gacaaaccca tggactccca
     2161 ggcgatgatg gagctctggc agaaattggc ccagcccggt gagccgcaca agacgtttgc
     2221 cggcttagcc ggaagttgga cgacgacgac caaggaatgg atggaaccgg gtaaaccgcc
     2281 cacggaagcg agcgggaccg ctgagatgaa gatgctactg gacggacgtt ttctctatca
     2341 ggagttcaag agccagatga tggggcagcc gttttcagga atcggcatcg atgcctatga
     2401 caacgtgcgc aaaaaatatg tgaccgcctg gatggacacc atggggaccg gcatcttcat
     2461 catggaaggc acggcgagtg cagacgggaa gacgatcacg ctgaaggggt cgcatcccga
     2521 accgggcggc gggcagatgc accatcgcgc agtctggaag ttagtggaca gcaatacgca
     2581 gacgttcgag atgtatggaa accacgggca cggcaaggaa gcgaagatga tggagatcac
     2641 ttataccagg aagccgtaag gtgcactctc ctaaaccctg tgacgtggga gcatcgagcg
     2701 gcgtggacag tgtgaaggca tgaacactgt gccctgggca tagccgcatc agtggccggt
     2761 gagtcatcaa ttcttcgaag ggaggccgtt atgcaacgga cgatcggatt cctcgcgatg
     2821 gcactcattc tgacaagcct gagcggctgc agttatctct tttacccgcg agccggcgac
     2881 tatgccacgc aggcgaaggg ggcgaacggt gtcgagacca tgatgaacct ctccagcatg
     2941 atggaggcta ccgcgtccaa ggccaagggt ggaaagggtg tcgatacggc cttcgacgat
     3001 cttcacaatc agttccatgc cttgagtgat tcgttctgcg gtgtgacgga tgcacaaagc
     3061 aaaacaccgg cctacgcctt ggccgtgacg cacaagaagg aactcggtgc gatttttaag
     3121 cggctatgga agttcaaaga tgatcagcct cagcgagacc aacaccttga gttgttggcc
     3181 actgagttga aagaactccg tgagacgttg cagacgatca agtagggcca gtcgtagagg
     3241 atgatggcag catggtctgc cgggcggagg ctagttgaat cacctttgat acgtgctgcg
     3301 cgctctggca tggtcaagtg aggaagatgt tggaatttac ctagcctagg acgtcagagt
     3361 taagcatctt cagtcaggga acatgagtgg gaagagggtg gcgaatccag gcgtgggaga
     3421 ctgggatctc gcgccccctt cccatgatgg cgtccccacc gaaccacgtg tcgtctcccc
     3481 gctcggcgca accttcaagt ctcgtgcagt ccattcttga actaagggaa gagcactgtg
     3541 ccctgattgc ctacgagtgt ctgccacgca gatgagcgcc gaggctccgt tgcttacgca
     3601 ggagccaacc agtaagatag gcttccagta atatcccatg aactgcgtaa tcagatttta
     3661 tcagggagcg agcatggcca ggcgatcacg attttcaacc gtagccccga tgatatacgg
     3721 gtgcctggcg ttggcgctgc tctcaagctg taagcaggaa gtcggttctt cccaggcccc
     3781 tccggttcca gaggtcggca ttgtcgtggc gacggcgcag gacgtgcccg atgagccgga
     3841 gttcatcgga cagtcggagt catcgcggcc ggtggaaatc agatcgcagg tgacagggat
     3901 tctcaaagaa tggttcttta aggaaggccg cgacgtcaag aaaggcgacc gcctctatca
     3961 gatcgacccg gtgccctttc atgccgcaat gttgagcgcg aaggcgaaag tcgctcaggc
     4021 ggaggcgcga ctggtgcagg ccaagcagaa tctagcccgg gtgaagccct tgttggccga
     4081 gcaggccgtg agtacaaaag atgtcgatga tgcggtggcc gaggagatgg cggcgaaagc
     4141 gaacctcgaa ggcgccaagg cggaactggt caaagccaag ttcgatctcg acaacacgct
     4201 gatcgtggcg cccatcagcg gcatgatcga gcggacgcgt gtatatgaag gacgacttgt
     4261 gtcggcccag accgatctcc tgacgatcat tcagcaggtc gatcccatgt atgtgatcgt
     4321 cagcgcaccg gaaagttttc tattgaagcg gcagcgcgac agtacggcca aacggattca
     4381 gcaccccggc gtctatcaat tgcgcggcgt cctgaccttt gcggacggaa cgacgtacgg
     4441 gcaggagggg gtgttggacc tcctggatgt cggactcaaa accgatacgg gatcgcggca
     4501 ggcccgggta gtctttccca atcatgatcg cgtgctgctg cccgggcagt tcgtgcgggt
     4561 ccgcttcaag gggacggtca agaccggcgc gattctggtg ccgcagcgtg ctgtgcagca
     4621 aggcgccaag gggtccatcg tgtttgtcgt cgggaatgag gacaaggttg agatgcggga
     4681 ggttcaggcg accagctggc aaggcaacca atggattgtc gagcaagggt tgcatgtcgg
     4741 ggaccgcatc atcgtggaag gtctgcataa gattgcgccc ggcgcgccgg tgaagcctgt
     4801 gccgcttcct gctcctgccg cgacgaccgt tccgactgcc gccaagccgc agccggagcg
     4861 cgcctcgtga tctcccattt ttttatcgac cgcccgatct ttgcgtcggt tctgtccatc
     4921 gtgatcatgg tggtcggcct ggtagcgctg aaggcgctgc cggtcgctca atttcctgaa
     4981 atcacccctc ccgtcgtcca gatcgaggcc gattacccgg gtgccaacgc ggagatcgtg
     5041 gccgactccg tggcgcgtcc cattgaggtc cagcttccgg gcatcgataa tctgctgtac
     5101 tacgactcca ccagcaccaa cgacgggcac atgtcgatca agctgacgtt cgagatcggc
     5161 actgacgtgg acatcgccca agtgcaaacg cagaatcggg tgaaactcgc ggaaccgcag
     5221 ctgccgcctg aggtcgtgcg ccaggggatc agcatcaata aggtttctcc cgatttgctg
     5281 gcagtggtgg cgctaagctc catcgatccg acgcacgata cggtgtattt gtcgaactat
     5341 gcgattctcc gcgtgctcga caatgtgaaa cggttacgcg gagtgggcaa tgcggtggtg
     5401 ttcggctccc agaactattc aatgcggctg attctggatc ccatccggat ggcccagctc
     5461 agcttgacgc cgacggacat cgccaacgtt gtgcgtgagc agaaccggga ttttccggcc
     5521 ggaacgatcg gccgtgagcc ggcgttgaag ggcaccgagc tcaccattcc cgtcatcacg
     5581 cagggccgat tgacggaggt gaaggagttc gaggagctga tcgtccgtgc catgccgaat
     5641 ggatcgatga tccacctcaa ggacgtagcc cgtgttgaac tgggtgcaca atcctatacg
     5701 ctggaagggc gttggaacgg caaaccgaac gtgtttttgc tgacgttcct gtcgcccggt
     5761 gccaatgcgc tcgaaacggt aaagcgggtt cgggctgaac tggc
//
LOCUS       DAORQN010000084         5753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_592_length_5753_cov_11.106353, whole genome shotgun sequence.
ACCESSION   DAORQN010000084 DAORQN010000000
VERSION     DAORQN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_5753_cov_11.106353"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..918)
                     /locus_tag="PK614_06015"
     CDS             complement(358..918)
                     /locus_tag="PK614_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRB81802.1"
                     /translation="MSIPLGKGILLVAAPALNDPNFRQTVVLLCEHGPEGALGVIVNR
                     PTAMSISEALPQVPILEGQPHVLYSGGPVQTNQVMMLYRINQTPENSHQVFDGVCLGG
                     DLEIMERILMEQPGQESFRAYLGYSGWGPGQLESEMQVGSWITLPADPSIVFEKEPTR
                     IWSDIFLSLDDTSRHYADMPFDPTSN"
     gene            complement(983..1774)
                     /locus_tag="PK614_06020"
     CDS             complement(983..1774)
                     /locus_tag="PK614_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRB81803.1"
                     /translation="MRVGYLQFDPVFGEVAHNLDLITARLEEAEADLLVLPELCASGY
                     QFVSQEEVFRLAEPVPDGATTKRLVEIAARRGMTIVAGLPERDGRHCFNSAVVVGPRG
                     FIGCYRKTHLFFEETLWFTPGDSGFHVWDIGMAKVGVMICFDWYYPESARTLALQGAD
                     IIAHPSNLVLPHCPDSMVTRCLENRVFSVTANRIGSEARGGKDRLTFIGMSEVVSPRG
                     RILHRAPRETEDLTIVEIDPAEARIKALNDYNDLLRDRRPALYGD"
     gene            complement(1859..2215)
                     /gene="smbP"
                     /locus_tag="PK614_06025"
     CDS             complement(1859..2215)
                     /gene="smbP"
                     /locus_tag="PK614_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HRB81804.1"
                     /translation="MQTAKWRGVVVAGVMMALVGAPMVAGVAFAAGNKHQAEAVEHAK
                     EAVAHGKQGHADALVKHAEAALKHAEGAVAETKNPHVTEAIKGLKDGIEHGKAGHADV
                     ATKAVENALPHLSEGM"
     gene            complement(2296..3189)
                     /locus_tag="PK614_06030"
     CDS             complement(2296..3189)
                     /locus_tag="PK614_06030"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRB81805.1"
                     /translation="MKNPLLIDCDKLQKRLGQPGLVVLAVRGRAAYEFGGHIPGAVHS
                     TWHEYSDPDAIAKGLLDPDLKRIEKRVQALGINQDSEVVIYSNPFDNWGDEGRMFWML
                     EYLGHKNLKVLDGGWVKWIHERRPFEHGAVRCAPGNFVVSPVESAIIMKNELKGIARK
                     PNPTTTIVDARSLEEYLGKEISGIPRPGHIPGAVHVAWSGFLNADATVKDLAAINAQL
                     DLKGLNPTHETICYCTGGVRSAWLYFILRLAGYQRLRNYPGSWWEWSRDFACPVEKDL
                     LALQKLLGLSESQVPTGMRPS"
     gene            complement(3218..4492)
                     /locus_tag="PK614_06035"
     CDS             complement(3218..4492)
                     /locus_tag="PK614_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81806.1"
                     /translation="MNLWVKILLGVAGLAGAIYLYYTEVKPVVIFGLRSDYAHAIPFQ
                     KVPEGLTSLKAESCGQCHREIYEEWKTSIHAHAYEDPFFQAYWKKDKNIWVCLNCHTP
                     LENQQPTLVKDIPRGRVEKATQEPNPHFDADLRKESITCAACHVRDGVILGPFDDSAA
                     PHPTKFDPSFRNAQFCSRCHNVVSGPAQFYNVGPCGTYAEYEGKYFMQERGFICQSCH
                     MPEVDRPVAENGPIRRGRRHLWRGGHDPDMVKRAVAIQVKADPPAPKPGEQVTVTLSL
                     MNAGAGHKIPTGDPDRHFTVEFTVQDSSRQVLAEKSHTMGRWIMWQPAIVELYDNRLL
                     PLASRDYQFAYRMPEASKGLTLKTRVRYHILTDGQHEMLKAKYGLMADDPYAFTVYER
                     EVPLNETLAAALVDPLPEPPPMACASPTVSKG"
     gene            complement(4489..5484)
                     /locus_tag="PK614_06040"
     CDS             complement(4489..5484)
                     /locus_tag="PK614_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRB81807.1"
                     /translation="MSKYEIQTTLHVSRTFLMGTTGLIYDPRYLEHDMGAGHPESPNR
                     LRAIMQRLEQSGTLATLTRIDPRTAEDEWVTLVHRPEYVAMLNRQAPTHGRVSLDADT
                     SMSPGSLGATYLAAGGALAGVDAIMAGRVQHAFCAVRPPGHHAEADRAMGFCLFNNVA
                     IAARYAQKRYGVQRVLIVDWDVHHGNGTQHSFESDPSVLFFSTHQYPHYPGTGRASEC
                     GRGAGEGLTINVPMEAGEGDDEYRAVFQKVLVPAADAFKPEFVIISAGFDAHRDDPLA
                     SMGLTEEGYADLTKIVAGIARQHCQGRLLSALEGGYNLTALAASVERHIQALVEA"
BASE COUNT         1220 a         1801 c         1588 g         1144 t
ORIGIN      
        1 tcctgcgagg agaaaccgaa tcacgcggat atcaagaaat cgttttgggt cgaactgcac
       61 ccagaactcc tagattatcc gtagcgtccc cacaaaaagg gcagcaagct agaaatacaa
      121 aagaatcaag ttctgcctat tcagtagcca aggtattaac agaagagagc gacgacaacg
      181 tcttgcacta tgagttcacg catgcggggt ctcattcatc cacacaagaa tgcgacaagg
      241 acacccgatc agattgccgg caataaggga agtaggaatc acgatctcag ccacatcgcc
      301 gtcactgaaa gcccttgaga agcgcatatt gaacagacag gcgggttcaa ggtctgatta
      361 attcgaagtg ggatcgaacg gcatgtccgc gtaatggcgg gaggtgtcgt ccagcgagag
      421 gaagatatcc gaccaaatgc gggtcggttc tttttcaaac acaatcgagg gatcggcagg
      481 caacgtgatc caggaaccaa cctgcatttc cgactccagc tggccgggcc cccagcccga
      541 ataccccagg tacgctcgaa acgattcctg cccgggctgc tccatgagga tccgttccat
      601 aatctctaag tcgccgccaa gacagacacc gtcaaacacc tggtgcgaat tttcgggcgt
      661 ctgattgatg cgatagagca tcatcacctg attggtttga acggggccgc cggaatacaa
      721 cacatgcggt tggccttcca ggatcggcac ctgcggaagc gcctcagaga tggacatggc
      781 ggtcggacgg ttgacgatca cacccagcgc cccttccggc ccatgctcac acaggagcac
      841 caccgtctgc cggaagttgg gatcgttcaa cgccggcgcg gccacgagga gaatgccttt
      901 accgagtgga attgacatgg ttcatcctac ctggtgctcg taagtgacgc aagttgagtc
      961 atgaaacgta tacgacgcgg gattagtcgc catacaaggc tgggcgccgg tcgcgcagga
     1021 gatcgttgta gtcattgagt gctttgatcc tggcctcggc ggggtcgatc tcgacgatcg
     1081 tcaggtcctc ggtttcacgc ggtgcgcgat gcaggatccg gccgcgtgga ctgacaactt
     1141 cgctcatgcc gataaacgtc agccggtcct ttcctccgcg cgcttcgcta ccgatccgat
     1201 tcgccgtcac gctgaacact cgattttcca aacagcgggt taccatggaa tcaggacaat
     1261 gtggcagcac caggttcgac ggatgggcga tgatatccgc gccctgcaac gccagcgtgc
     1321 gggcggattc cggataatac cagtcgaagc agatcatgac gccgactttg gccatgccga
     1381 tatcccacac gtgaaacccg ctgtcgcccg gagtaaacca gagcgtctcc tcgaaaaaga
     1441 gatgcgtttt ccgatagcag ccgatgaatc ccctgggtcc gaccacgacc gccgaattaa
     1501 aacagtgtcg gccgtcccgc tccggcaacc cggctacgat ggtcatccca cggcgtgcgg
     1561 caatttccac aagccgtttc gtggtcgccc cgtccggcac cggctccgcc agccggaaca
     1621 cctcttcctg cgacacaaac tgataacccg acgcgcacaa ttcaggcaac acaagaaggt
     1681 cggcctccgc ctcttcaagt cgagccgtga tgaggtcgag attgtgcgcg acctcgccga
     1741 agaccggatc aaattggaga tacccgaccc gcatgatgtc gcctttctac tcaaagaaaa
     1801 acgggcaggg acggccctgc ccgtttcatc ttacacgccg tcgacgcgca agaaccgatt
     1861 acataccttc cgacagatgg ggaagagcgt tctcaacggc tttggtcgcc acatcggcgt
     1921 ggccggcctt gccgtgctcg atcccgtcct tgagcccctt gatagcttca gtgacatgcg
     1981 gattcttggt ttcggccacc gcaccctcgg catgcttgag cgccgcttcg gcatgcttca
     2041 ccaaggcgtc cgcatggccc tgcttcccat gcgccacggc ttctttggca tgctccaccg
     2101 cttcggcctg atgcttattt cctgccgcga aagccacacc ggcaaccatc ggcgccccga
     2161 cgagcgccat catcacaccg gctaccacga ctccccgcca tttcgcagtc tgcatgaaca
     2221 gcctcctctg taagtatgta caccgcatga ttccaacgat actacatttc agcataaaga
     2281 ggggcctgca tcctgtcaag acggacgcat gccggtcgga acctgcgact ccgacaggcc
     2341 gagaagtttc tgcaacgcga gcagatcctt ctcaaccggg caggcgaaat cgcggctcca
     2401 ctcccaccag gagcccggat aattacgcaa ccgctgataa ccggccagtc gcaatatgaa
     2461 atagagccac gccgagcgca cgccgccggt gcagtagcag atcgtttcat gtgtcgggtt
     2521 cagccccttg agatccaact gcgcgttgat ggcggccaga tccttgaccg tcgcatcggc
     2581 attcaagaat ccgctccacg ccacatggac ggcaccggga atatggccgg gcctcggaat
     2641 gccggagatt tctttcccca gatactcttc caggctccgc gcatccacga tcgtggtcgt
     2701 cgggttcggc ttccgcgcaa tccccttcaa ctcgttcttc atgatgatcg ccgactctac
     2761 aggggacacg acgaagttgc cgggtgcgca gcggaccgcg ccatgctcga acggccggcg
     2821 ttcatggatc catttcaccc acccgccgtc cagcactttg aggtttttgt ggcctaagta
     2881 ttccagcatc cagaacatgc gtccttcgtc gccccaatta tcgaaggggt tcgaatagat
     2941 gaccacctca ctgtcctgat tgatgccgag cgcctgcacg cgcttctcga tgcgtttcag
     3001 gtccggatcc agcaacccct tggcgatggc atcgggatcg ctgtattcat gccaggtgga
     3061 atgcacggca ccgggtatat ggcctccgaa ctcataggcg gccctccctc gcacggcgag
     3121 cacgacaagg ccaggttgcc ccaagcgttt ctgaagctta tcacaatcga tgagtaatgg
     3181 atttttcatt ggttcataca tctcggtggg tgatgactta ccctttggat acggtcggac
     3241 tcgcacaggc catcggcggc ggctccggca agggatcgac caacgcggcc gccagcgttt
     3301 cattcagcgg cacttcccgc tcatagaccg tgaaggcgta gggatcgtcc gccatcaggc
     3361 cgtactttgc cttcaacatc tcatgctgcc catcggtcag gatgtggtac cgaacccggg
     3421 tcttcaacgt caaccccttg gacgcctccg gcatccggta ggcaaactga tagtcccggc
     3481 tggccaaggg gagcaagcgg ttatcgtaca actctacgat ggccggttgc cacataatcc
     3541 agcggcccat cgtgtgggat ttctcggcca gcacctgccg gcttgagtct tgaaccgtga
     3601 attccaccgt aaagtgccgg tcgggatcgc cggtcggaat tttgtgcccc gccccggcat
     3661 tcatcaggga gagggtcaca gtgacctgct cacccggttt tggagccgga ggatcggcct
     3721 tcacctgaat cgccacggcc cgcttcacca tgtcgggatc gtgccccccg cgccagagat
     3781 gccgccgccc gcgcctgatc ggcccgtttt cggccaccgg ccgatcgacc tccggcatat
     3841 ggcaactttg gcaaatgaag ccccgctcct gcatgaaata cttaccttca tattcggcgt
     3901 aggtcccgca ggggccgacg ttgtaaaact gcgccggacc ggagaccacg ttatggcaac
     3961 gcgaacagaa ctgcgcgttg cggaaactgg ggtcgaattt cgtgggatgc ggcgcagcgg
     4021 aatcgtcgaa ggggccgagg atcactccgt cgcgcacatg gcaggcggca caggtgatcg
     4081 attccttccg caggtcggca tcaaaatgag gattcggttc ctgcgtcgct ttctcgaccc
     4141 gcccccgggg aatgtctttc accagcgtcg gctgttggtt ctccaacggc gtgtggcaat
     4201 tcaaacagac ccagatgttc ttgtcctttt tccaataggc ttgaaagaac ggatcttcat
     4261 aggcatgagc gtggatgctg gttttccact cctcataaat ttcccggtgg cattgaccgc
     4321 aggactcggc cttcaggctg gtcaggccct ccggcacctt ctggaacgga atggcatggg
     4381 cataatccga ccggagtccg aaaatgacga ccggtttcac ctcggtgtaa tagaggtaga
     4441 tcgccccggc gagacctgcg acgcccagca gaattttcac ccacagattc atgcctccac
     4501 cagcgcctgg atatgtcgct cgaccgacgc ggccaacgcc gtgaggttgt acccgccttc
     4561 gagtgccgac agcaggcgtc cttggcaatg ttgcctggca attccggcta caatcttcgt
     4621 cagatcggca tacccttctt cagtcaaccc catactcgcg agcggatcgt cccggtgggc
     4681 gtcaaaaccg gcggagatga tgacaaactc cggcttgaag gcatcggcag ccggcaccag
     4741 aaccttctga aacaccgccc ggtattcgtc gtcgccttcc cccgcctcca tcggcacatt
     4801 gatcgtgagc ccttccccgg ctccgcggcc gcactcgctg gcgcgcccgg tcccgggata
     4861 gtgcggatac tggtgcgtac tgaaaaacaa caccgacgga tccgattcga aactgtgctg
     4921 cgtcccgttc ccgtggtgaa cgtcccaatc gacgatcagg acccgttgca cgccataccg
     4981 tttctgcgca taccgcgccg cgatcgccac attgttgaac aggcagaaac ccatcgcccg
     5041 atcggcttcc gcatgatgcc ccggcggacg gaccgcacag aaagcatgct ggacccggcc
     5101 ggccataatc gcatccaccc cggccagggc tccaccggcc gcgagatagg tggcgccgag
     5161 cgatccgggt gacatggagg tatcggcgtc gagcgagacc cggccatgcg tcggcgcctg
     5221 ccgattgagc atcgcgacat actccggccg atgcaccagc gtgacccact cgtcttcggc
     5281 tgtgcgcggg tcgatcctgg tgagtgtggc gagcgtaccg ctctgttcga gccgctgcat
     5341 aatcgcgcgc agccggttgg gcgactccgg atgtccggcg cccatgtcgt gttccaggta
     5401 acgtggatcg taaatgagcc cggttgttcc catgagaaaa gtccgtgaaa cgtgaagcgt
     5461 cgtttgtatc tcgtatttcg acacaaacag tttcctcacc ctatcatgca ggaaagagcg
     5521 tagacaatga gcggaaacgc gtgctacggt ttcgcttcac cgtgccgatc gaccctggag
     5581 aagccaatgc cgaatccgag aatcgaaccg ctgaaacgag tgctggccct cgaacccgac
     5641 gatgacgtcg cctggttcgg actcggaaaa gcctatatgg aagatgccaa ttttgaagag
     5701 gctgccaagg ccttgagaca gtgcatcacg gtcaagccca cctactccgc cgc
//
LOCUS       DAORQN010000085         5740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_594_length_5740_cov_101.553034, whole genome shotgun sequence.
ACCESSION   DAORQN010000085 DAORQN010000000
VERSION     DAORQN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_594_length_5740_cov_101.553034"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..751
                     /gene="pilO"
                     /locus_tag="PK614_06045"
     CDS             128..751
                     /gene="pilO"
                     /locus_tag="PK614_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRB81808.1"
                     /translation="MSLNAISLDGLRSIPTGQKVALLGLLVAAILVGFYFYVVDPKTM
                     ELEAVQGQVAQLDTEIQNLTLKVKHLDELVAASKQLEIELAAKKERLPPEEEAVMLLK
                     QVSDLGMRLGLDVRLWKPGPQAEDPSKLFIRMPINVEVAGGYHTAAIFFDRISKLSRI
                     VTVQDVRIGAARVDQGRVVTQTVFDLVAYAAPGEKKAAAPVPAAKPK"
     gene            793..1398
                     /locus_tag="PK614_06050"
     CDS             793..1398
                     /locus_tag="PK614_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HRB81809.1"
                     /translation="MKRRIWWRGSMAVAAVLLVSGGAVSPVEAKTLPHLRQVASMRPA
                     DSLKVMPLPEGKRSGPAVEAPASRSEAAVSASGDSLSTEFSGTSYDPSGRRDPFLPMI
                     QLGQQTEQDVTLPPLQRVGLTELSLIGVLWGNYGYTAMVQTPDGKGYSIRRGTRIGPN
                     NGVVSSITERGIIVQERFTDVYGNKQEREYVKLLHPKEGTE"
     gene            1395..3389
                     /gene="pilQ"
                     /locus_tag="PK614_06055"
     CDS             1395..3389
                     /gene="pilQ"
                     /locus_tag="PK614_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRB81810.1"
                     /translation="MKHAQVGVHPLLSVTVMAGVLGLAGYVQAAAPGETTGLTGFTQA
                     TDQSAALSVESATPVPERAAAAGSAATMLTKVEAKPDGSRMALILSGNGVFTHTVKMI
                     GDRRMVVDMPHIQSDGHQSQLMVSHALLSRVRFGYHPDKVRLVLDLSQSVEHTIDSTD
                     GRLVLTLEPKAAASVVETKTVDGGALAHVEASAEQTGSLEPKQPTSHPTLVTHKSPKA
                     LFRIQPIQLTAESVQKEDRKPESNEVVNGASRFVGRRISLDFQQADISNVLRLIAEVS
                     GFNIVVGEGVKSKVTMKLANVPWDQALEMLLKMNALGMIRQGNIVWVDTLQNIAKQQD
                     EEARAKDSKAKAEPIVTRVFYIRNLNATEVQTSLRQNLSPRGTMTVSAASNALIISDT
                     ESKLEVLRQLLDGVDLEVPQVQIEARIVQADTTYTRSLGVQWGIQNVNQLGSSSGTSA
                     FKTGTTGAFGAQVSDFLINLPANPGLPSVPGAGFSIGKTDGAMLDVRLSAGELLGLTK
                     VIAAPKITTLDKRDAKIAQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEI
                     GKQILMKVRATRNAVGARSNPAGPSIDRREATTQVLVRDGETMVIGGVFVDTQSNNVA
                     GIPYLSRIPVLGWLFKNKTENVSKQELLIFLTPTIVRSTT"
     gene            3662..4819
                     /gene="aroB"
                     /locus_tag="PK614_06060"
     CDS             3662..4819
                     /gene="aroB"
                     /locus_tag="PK614_06060"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRB81811.1"
                     /translation="MTRSSIDRPSHSIHVELGARSYAIVIRRDLLREIGEELKRLKCA
                     GKVGVVTDRNLAKHYLTQVTRRLKAAGFTVVPVVLPPGERTKTLRSIGTVMDALVNAR
                     FERSSTLLALGGGVIGDITGFAAAIYQRGIPFVQVPTSLVAQVDSSVGGKTGVDHPKG
                     KNLIGAFNQPRAVFIDPAMLATLPPREWVAGLAEVIKYGVIADAAFFEYLEQNMESIQ
                     KLTDDAVAHIVKRSCEIKAQVVSEDEREADRRRILNFGHTIGHALESLGGYRGFIHGE
                     AVAVGMVYEADLARHLGYCGAEVVARLRALVKAAGLPDRLPKVAFNALWGAMQQDKKV
                     SAGTIFCVVPEQIGAVRIVPLEKATTRAWFQDVHTREAAELKRARPVGRAH"
     gene            4827..>5740
                     /locus_tag="PK614_06065"
     CDS             4827..>5740
                     /locus_tag="PK614_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB81812.1"
                     /translation="MATKRTLTQVEQALREKTREVDVLHRISESISNTLDLEAVLRHI
                     VDMVVEVTKADACLLYLLSDNRDELILRASKNPHPKLIGRITIGMGEGITGWVARERT
                     RVVIPNSASDDTRFKFFHNLPEDRYQAFVSVPITTKQEVVGVINVQHKRSRRYRPDEL
                     ALLSTIATQVGGAIENARLYDQMTRKALQLDTLSQVSETVSSNRLIEDVLQLIVTMTA
                     QMMGSKICSIILLDQPTGELRIAATQSLSEQYRRKPNVKIGQSISGRAVQDRRPIIVP
                     DVIKEGTYMYPDMAAKEGLCSLLCVPMLV"
BASE COUNT         1192 a         1550 c         1845 g         1153 t
ORIGIN      
        1 gaattcgtga acaacctgcg gcggaccgat caattcggcg ccatcaagct gatggagagc
       61 cgggcgggac tggacaacaa gatgaacacc tatcaattca aactcgacct gtcgatgaaa
      121 ggctaacatg agtctgaatg caatcagctt agacggcctc cgcagcattc cgacaggtca
      181 gaaagtcgcg ctgctcggac tgttggtggc ggcgattttg gtagggttct atttctacgt
      241 ggtcgatccc aagaccatgg agctggaagc ggttcagggt caggtcgccc agttggatac
      301 cgagattcag aatctgacct tgaaggtcaa acatctggat gaattggtgg cggcgagcaa
      361 gcagctggaa atcgaattgg ccgcgaagaa ggaacgcctc ccgccggaag aagaggcggt
      421 gatgctgctc aagcaagtgt ccgatcttgg catgcgactg gggttggatg tccgtctctg
      481 gaagccgggg ccgcaagccg aggatccgtc gaagctcttc attcggatgc cgatcaacgt
      541 ggaagtggcc ggcggatacc atacagccgc catcttcttc gatcgtatca gcaagctgtc
      601 ccgtatcgtg acggttcagg atgtgcggat cggcgcggct cgtgtcgatc aaggacgtgt
      661 ggtcacgcag acggtgttcg atctggtggc ctatgctgcc ccgggagaga aaaaggccgc
      721 cgcgcccgtt cctgcggcga agccaaagtg aggattggag cctagcttat gcccaacgcg
      781 aggggacgca ccgtgaagag acgaatctgg tggcgcgggt cgatggccgt tgcagcggtc
      841 ctgctggtga gtggaggcgc ggtgtcgcct gtcgaggcga aaacactgcc tcacttgcgc
      901 caagtggcgt ccatgcggcc ggccgattcc ttgaaggtca tgccgttgcc cgaggggaag
      961 cggtctgggc cggctgttga agctccggcc tcgcgttccg aagcggcggt ctcagcatcg
     1021 ggggattcat tatcgacgga attttcagga acctcgtacg atccgtcggg tcgtcgggat
     1081 cccttcctgc ctatgatcca gctgggccag cagacagagc aggatgtgac tcttcccccg
     1141 ctgcagcgtg tcggcctcac ggagctgagt ttgatcgggg tgctctgggg caattacggg
     1201 tacacggcca tggttcagac gccggacggc aaagggtaca gcatccgccg cgggacacga
     1261 atcgggccca acaatggtgt ggtcagttca atcaccgaac gaggcatcat cgttcaagag
     1321 cggttcacgg acgtgtacgg gaataaacag gagcgggaat acgtcaagct cctgcacccg
     1381 aaagagggta cagaatgaaa catgcacagg ttggtgtaca tccgctgctg agtgtcactg
     1441 tgatggccgg agtgttgggg cttgccggat atgtgcaagc cgcggcgcca ggcgagacga
     1501 ccggactgac cggattcacg caggccactg accagagtgc ggccctgtcc gtggagagtg
     1561 ccacacccgt tccggagcgc gcagctgccg ccggctcggc tgcgacgatg ctgacaaaag
     1621 tggaagcgaa gcctgatggg agtcggatgg cgttgattct gagcgggaac ggcgtcttca
     1681 cgcataccgt gaagatgatc ggggatcgtc ggatggtcgt cgatatgccg catattcagt
     1741 cggacggcca ccagtcacag ctgatggtca gtcatgcctt gttgtcgaga gtccgattcg
     1801 ggtatcaccc ggacaaggtg cggttagtgc tggatttatc gcagtccgtc gaacatacca
     1861 tcgattcaac cgacggccgc ctggtcctca cgctggagcc gaaggcggcg gcgtccgtcg
     1921 ttgaaacgaa aactgtggac ggtggcgcct tggcccatgt tgaagcgtcg gcggaacaga
     1981 cgggttcgct ggaaccgaag cagccgacgt cccaccccac gttagtgacg cataaatcac
     2041 cgaaagccct gtttcgtatc cagccgatcc agttgacggc ggagtcggtt cagaaagaag
     2101 accggaagcc tgagagtaat gaggtggtga acggcgcatc ccgattcgtc ggccggcgta
     2161 tctccctcga ttttcagcaa gccgatatca gcaatgtgtt gcggttgatt gcggaggtca
     2221 gcggcttcaa cattgtggtc ggggagggtg tcaaaagcaa agtcacgatg aagctggcga
     2281 atgtaccgtg ggatcaggcc ctcgagatgc tgctgaaaat gaatgcgttg gggatgattc
     2341 gccagggcaa catcgtgtgg gtggataccc tgcagaatat tgccaagcag caggatgaag
     2401 aagcgcgggc aaaggactcg aaggcgaagg cagagccgat cgtcacccgg gtattctata
     2461 tccggaatct caacgccacg gaagtgcaaa cgtcgttacg ccagaacttg agtccgcgcg
     2521 ggaccatgac ggtcagcgct gcgagcaatg ccctcatcat cagcgacacg gagtccaagc
     2581 tggaagtcct gcggcagttg ctggacggcg tcgatctcga agtaccgcaa gtgcagattg
     2641 aggcccgcat cgttcaagcc gacacgacct atacccgctc actcggtgtg cagtggggta
     2701 tccagaacgt caaccagctg ggaagctcca gcggaacgtc tgccttcaag accggaacta
     2761 ccggggcatt tggcgcccaa gtctcggact tcctgatcaa cttgccggct aaccccggat
     2821 tgccgtcggt gccgggtgcc ggattctcga tcgggaaaac cgacggggcg atgttggatg
     2881 tgcggttgtc ggccggtgaa ttgctgggct tgaccaaagt gattgcggcg ccgaagatca
     2941 cgacgctgga caagcgggac gccaagattg cccagggtga atcgatcccg ttccagacca
     3001 cgtccttgca gggaacccag acgaccttcg tggatgcgaa cctggagttg aacgtgacgc
     3061 cgcaaattac ctctcgcgac ccgaaagaga tcggcaagca gattctcatg aaggtgcggg
     3121 ccacccggaa cgccgtcggc gcccggagca acccggccgg tccgagcatc gatcgtcgtg
     3181 aagcgacgac ccaggtgctg gtgcgtgacg gggaaaccat ggtcatcggc ggtgtcttcg
     3241 tcgatactca gtcgaacaac gtggcaggaa ttccctatct gtcacgtatc ccggtcctgg
     3301 gctggttgtt caagaacaag accgagaacg tctccaagca agagttgctg atcttcctga
     3361 cgccgacgat cgtgcgctcg acaacttgac aggtcccagc gcggcaagtt aataatcgcc
     3421 cccattgaag atggttcgct atctcaatgg gggcgaatcc tgaggaacgt gtcttctccc
     3481 cctatctggt agcgctcctt ccgggtgtct tcttcctcta gatatcgcag gttacgatcc
     3541 agtctctttc gcctcatggc ctatcggggt atctggattg ttcgcgtggc tcgcttcccg
     3601 ggccatgccg ccatcatgga cttgagactc agccctgcgt ccacacgctg cgtgatacct
     3661 gatgacacgt tcttctattg ataggccgtc ccactccatt catgtcgaac tcggtgcgcg
     3721 aagttacgcc atcgtcattc gccgcgacct tcttcgggag atcggggagg agctgaagcg
     3781 tctcaaatgc gcgggcaagg taggggtcgt gaccgatcgc aatcttgcca agcactatct
     3841 cacgcaggtg actcgcaggc tgaaggccgc cgggtttacg gtcgttcccg tcgtgctgcc
     3901 tcccggggag cggacaaaaa cccttcgctc catcgggacg gtcatggatg ccctcgtgaa
     3961 tgccagattt gagcgttcct cgacgttgct ggccttgggt gggggtgtca tcggcgacat
     4021 cacgggattt gccgccgcaa tctatcagcg tggcatcccg ttcgtgcaag tgccgacgag
     4081 tctggtggcc caggtcgatt caagtgtcgg cgggaagacg ggtgtcgatc accccaaggg
     4141 gaagaatttg atcggggcct tcaaccagcc gcgcgcggtg ttcatcgatc ctgcgatgct
     4201 ggcgactctg ccgccgcgcg agtgggtggc tggtctggct gaggtcatca aatatggtgt
     4261 gattgcggac gcggccttct ttgagtatct ggaacagaac atggaatcga tccagaagtt
     4321 gaccgatgac gccgttgcgc acatcgtgaa gcggtcctgt gagatcaagg cacaggtggt
     4381 gtcggaggat gagcgtgagg ccgaccgacg ccgcattttg aacttcggcc atacgatcgg
     4441 gcatgcgttg gaatccctcg gcgggtatcg gggcttcatc catggggagg ccgttgcggt
     4501 cgggatggtt tatgaggccg acctggccag gcacctcggc tattgtggag cagaggtggt
     4561 tgctcgattg cgcgcactgg tgaaggccgc gggtttgccg gaccggttgc cgaaggtcgc
     4621 gttcaatgcg ttgtggggtg ccatgcagca ggacaagaag gtgtctgccg gaacgatttt
     4681 ttgtgtggtg ccggagcaga tcggggccgt gcgcattgtt ccattggaga aggccacgac
     4741 gcgcgcctgg tttcaggatg tccacaccag agaggcagcc gaattgaagc gtgcaaggcc
     4801 cgtcggacgg gcacattagg agtggaatgg cgactaaacg aacgctgact caagtcgagc
     4861 aagccttgcg tgaaaagacg cgtgaggtgg atgtcctcca tcgcatcagc gagtcgatca
     4921 gcaacacgct cgacttggag gccgtgctcc ggcatatcgt ggatatggtc gtggaagtga
     4981 ccaaggccga cgcctgcttg ctgtatttgt tgtcggataa cagggacgag ctgatcctgc
     5041 gggcctccaa gaatccgcat ccgaagctga tcgggcggat caccatcggc atgggcgagg
     5101 gcattaccgg ttgggtcgcg cgggagcgga cgcgtgtcgt cattcccaac agcgcaagcg
     5161 atgacacacg gtttaagttc tttcacaacc ttccggaaga ccgctaccag gcgttcgtct
     5221 cggttccgat tacgaccaag caggaagtcg tcggcgtcat caacgtgcag cacaaacggt
     5281 cgcgccggta tcggcccgac gaattggcgc tgctctcgac cattgccacg caggtcggag
     5341 gggccattga gaacgctcgc ctgtacgatc aaatgacgcg gaaggcgttg cagctggata
     5401 cgctgtccca ggtctcggag accgtgtcgt cgaatcgatt gatcgaagac gtgctgcagt
     5461 tgattgtgac gatgacggct caaatgatgg gatcgaagat ttgctccatc attcttttgg
     5521 atcagccgac cggtgaactt cggatcgccg ccacacaaag tctgagcgag cagtatcggc
     5581 gcaagcccaa cgtgaagatc gggcaaagca tcagcgggcg tgccgttcag gatcgtcggc
     5641 cgatcatcgt gccggatgtc atcaaagaag gcacctatat gtatccggac atggctgcga
     5701 aggagggcct ctgctcgctc ctgtgcgtgc cgatgctggt
//
LOCUS       DAORQN010000086         5739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_595_length_5739_cov_7.728536, whole genome shotgun sequence.
ACCESSION   DAORQN010000086 DAORQN010000000
VERSION     DAORQN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_595_length_5739_cov_7.728536"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..1969)
                     /locus_tag="PK614_06070"
     CDS             complement(326..1969)
                     /locus_tag="PK614_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRB81813.1"
                     /translation="MKHEHGHNHAHQHNHGDEHACCSIKGAGKADVSLVKDPVCGMMI
                     DPKTAKGGSSVHQGETYYFCNPKCKAKFEAEPEKFLGAPRPASAEDKEKIYTCPMHPE
                     IRKKGPGNCPICGMALEPEEVSLEEVENPELVDFTKRLKVSAVLAIPLALLAMSDLIP
                     GQPVQHAVPGWLNGIIQFLLATPVVLWGGLPFFERGWASIKSRNLNMFTLISIGTGVA
                     YGYSVVATFFPSIFPENLKGHGGMVPLYYEAAAVITALVLLGQVLELRARSQTGNAIR
                     ALLGLAAKTARRVKKDGSEEDIPMEHIHVGDHLRVRPGEKIPVDGKLIEGRSSVAEVM
                     ITGEPIPVEKEIGSSLTGATVNGQGSFIMEATRVGSDTLLSQIVKMVSQAQRSRAPIQ
                     KLADTVSGYFVPAVVVAAIITAFVWLFFGPEPALTYAIVNSVAVLIIACPCALGLATP
                     MSIMVGTGKGATHGVLIKSAEALEVMEKITTLVVDKKLPGAGGGAGDVSRLQLHRVDV
                     GYKISINSGDLCRTVELQNFICCRTDVDTQGSNRGEGAA"
     gene            2187..2582
                     /gene="cueR"
                     /locus_tag="PK614_06075"
     CDS             2187..2582
                     /gene="cueR"
                     /locus_tag="PK614_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRB81814.1"
                     /translation="MNIGEAAKASGVNAKLIRHYESIGIIPKATRSDAGYRVYSDADV
                     NILSFVKRARGLGFSMKEIKKLVGLWRNKSRSSSDVKNLALTHVKEMEQKIAELQSMV
                     KTLNHLAKFCHGDNRPDCPIIDSLAKEKS"
     gene            complement(2728..3447)
                     /locus_tag="PK614_06080"
     CDS             complement(2728..3447)
                     /locus_tag="PK614_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81815.1"
                     /translation="MVPSKPIVRWRALILVLFSLTLGLTGCQKDPQEIVSNNKDNKFD
                     IHQVEDDFEFLIGLDKVSAFACVKANAAQLRVVNQGVAEVNHFRKHCAEATSGSAWCE
                     QLVRPNPASRATFDCTYSASQSHYLIHPNQSTWKHAFAAVNLIGELETKGIRVCQIYN
                     WWRPEPYNANVGGAPGRHPYGTSVDVRFCSMSDVNRAFLELCKMRKAGRLRALGYYGT
                     TALHFGIGDSLANTWGRSCPK"
     gene            complement(3678..3911)
                     /locus_tag="PK614_06085"
     CDS             complement(3678..3911)
                     /locus_tag="PK614_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81816.1"
                     /translation="MKLFLPLNALSGLKLYFVIESFFSLAGSWARKRFVCFLKMLNLQ
                     IEHLMKSRLSFPAKGEEGWTTLSRKGRDPVGSK"
     gene            complement(3952..5286)
                     /locus_tag="PK614_06090"
     CDS             complement(3952..5286)
                     /locus_tag="PK614_06090"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRB81817.1"
                     /translation="MILRTVSFLAVIACLVGCGGGSYQSEKGSSDNQSQDGCPGPNCV
                     ADAPPQDLTPIPPNAIMVSNATELTAALQSAVGGETIALASGDYGQYTINGRMYPSMV
                     VIRPQQNASPVFKSIHIQNSRFIQISGVSVLPRVASGDNVSTAVSISGQDIVFEKSMV
                     NYIDDSSSWVASNWLNHARDGVGVSGTRITVRENVIKNIAFALSVDATNSLISRNKID
                     GFRGDGMRGLGDGTVFEYNDVRNCRQVDDNHMDGFQSWSNGPGGPGSGVVTGIVLRGN
                     YFLESDTPNPIVTCNLQAIGLFDGMFKDWVVENNVVVVNHWHAISFYGAINVKIANNT
                     VIDPNLTDNIRPWIMITSHKNGTSSQNSIIRNNIAPTIDPGSGSTADHNLVSNAGNLS
                     QIFANPVSLDYRLKLGSIAIDAGSSVNPPLLDFLGMSRPVGAGIDIGAHEYR"
     gene            complement(5512..>5739)
                     /locus_tag="PK614_06095"
     CDS             complement(5512..>5739)
                     /locus_tag="PK614_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81818.1"
                     /translation="EMWDAEKESSGASPARTEITLTFSSGQSYRVGRYPIYSVSKLEK
                     LFSLTREKGKKQSYDIKSGKYYDFVKPLGEG"
BASE COUNT         1508 a         1418 c         1297 g         1516 t
ORIGIN      
        1 gaagttgcaa gcgcgagacg tcgccagcac cgcccccggc gccgggcaat ttcttggcta
       61 caacgcgacg acttctgctt gggagccgat gaccggtaac tctggctcac tcactggtgt
      121 ggtgagtggt acggggctcg ttgcaggtac ggttagttct tacgggacta ttaatataga
      181 tgttggttct accgctaata agatcgtgca gctgactagt ggcgcacagt atccgcaggc
      241 cgatggatac ctgatcacta acatcaattc ggcaagcatc tcaggcgttg gaactgtcac
      301 tagcgttaat tctggcaccg ggcttttagg cggccccttc tccacgattg gaaccttgag
      361 tgtcgacgtc ggtacggcag caaataaaat tttgcaactc aacggttcgg cacaaatccc
      421 cgctgttgat ggaaatcttg taaccaacgt caacgcggtg aagttgcaag cgcgagacgt
      481 cgccagcacc gcccccggcg ccgggcaatt tcttatcaac cactagtgtt gtaatcttct
      541 ccatgacctc gagagcttcg gcgctcttaa tcaacactcc gtgagttgca cctttgcctg
      601 ttccaaccat gatcgacata ggtgttgcaa gcccaagcgc acagggacac gcaatgataa
      661 gcacagcaac tgaattcaca atagcgtaag tgagcgcggg ctcaggccca aaaaatagcc
      721 acacaaatgc tgtgatgatt gcggccacta cgacggctgg cacgaagtag ccagataccg
      781 tgtctgccag cttttgaata ggtgcacggc ttcgctgggc ctgagagacc atctttacga
      841 tttgagaaag aagcgtatct gagccaacgc gtgtcgcctc catgatgaac gatccctgac
      901 cgttgacagt ggcgccagta agggaacttc cgatttcctt ttcaaccgga atgggctccc
      961 ccgtgatcat gacttcagca actgagcttc gtccttcgat gagttttccg tcgaccggga
     1021 ttttttcacc gggccgcaca cgtaaatgat ccccaacatg aatatgctcc atcgggatgt
     1081 cttcttcgct gccgtctttc ttaactcgtc ttgcggtttt tgcagcaagg cctagcaaag
     1141 ctcgaatggc atttcctgtt tggctgcgtg ctcgaagttc caaaacttgt ccaagcagga
     1201 cgagtgctgt gatgacggcg gccgcttcat agtagagcgg gaccatacca ccatgacctt
     1261 tcaaattttc cgggaagatg ctgggaaaaa aagtggcgac gacgctatag ccataggcaa
     1321 cgccggtacc aatcgaaata agcgtaaaca tattaagatt tctggacttg attgaggccc
     1381 aacctctttc aaaaaatggc aagcctcccc ataagactac aggcgtggcc agcaagaatt
     1441 gaataattcc atttaaccat ccgggaactg cgtgttgaac gggttgacca gggatcaagt
     1501 ctgacatcgc aagaagtgct aagggaatcg caagcactgc actaacttta agtcttttcg
     1561 taaaatcgac gagctcagga ttttctactt cctcgaggct tacctcttcg ggttcaagcg
     1621 ccatcccgca aatgggacaa ttgccgggac cttttttacg gatctctggg tgcatggggc
     1681 aggtataaat cttttcctta tcctcagcag atgcgggcct aggagcgccc aagaattttt
     1741 caggctctgc ttcaaatttg gctttacact ttgggttgca aaaataatag gtttcacctt
     1801 gatgtacgct tgagccaccc tttgctgtct ttggatcgat catcattccg catacggggt
     1861 ctttaactaa actcacatct gctttgccag ctcccttaat agagcagcac gcgtgttcat
     1921 ccccatgatt gtgctggtga gcgtgattgt ggccatgttc gtgtttcata ttttctccaa
     1981 agtcggcagg gcgtaatcgt cctcctgcca tgtgcttcta tcatcggcct tccaatcact
     2041 ggaaggtcaa gccctttaaa aggtttattg ttaaacatgc ggctctaaca cactttacgc
     2101 ctcgcggctc acccgaattg agacaacccc cttgaccttc cgactgttgg aagctgtaag
     2161 gtacctttaa ggttgaggtg tgtcagatga acataggtga agccgcgaaa gcctctgggg
     2221 tgaatgcgaa gttgattcgg cattacgagt cgattggtat tattccaaag gctactcgaa
     2281 gtgacgcagg ctacagagtt tattcagatg ccgacgtaaa cattctttca tttgtaaagc
     2341 gggcgcgagg tcttggattt tcaatgaagg agataaagaa gctcgttggt ctttggcgaa
     2401 acaaatcgcg aagtagttcg gacgttaaaa atctggctct aactcacgtg aaggagatgg
     2461 agcaaaagat tgctgagctt caatctatgg tgaaaacgct taatcacctc gccaaatttt
     2521 gccacggcga taatcggccg gattgtccga ttattgattc cctggcgaag gagaagtcat
     2581 gaatattata acggcgatca tcctttcctt attttctgct cagatatttg caaagaccgt
     2641 aaagtacgag gctcagtctc cgcgccagca tggccatcca gaacgttgat tcttgcattt
     2701 tcttttcctg ctgcaaggtg ctgtaaatta cttcgggcag gagcggcccc atgtattggc
     2761 aaggctatct ccgatgccaa agtgaagagc cgtggtgccg taatagccaa gtgctctgag
     2821 gcgtccggcc tttctcattt tgcaaagttc taagaacgca cgattgacgt cgctcattga
     2881 gcaaaacctg acgtcgacag atgttccgta tgggtgacgc ccgggcgccc cgccgacgtt
     2941 tgcattataa ggctccggcc gccaccaatt ataaatttgg caaacacgaa tgcctttggt
     3001 ttccagttcc cctattagat ttaccgctgc aaacgcatgt ttccaggtac tttggttagg
     3061 atggatcaga taatgggatt gcgatgcgct ataggtgcaa tcaaaagtcg cgcgacttgc
     3121 gggatttggg cgaaccagtt gttcgcacca tgctgacccg ctggtagcct ctgcacaatg
     3181 ttttcgaaag tgattcactt ctgcaacgcc ctgatttacg acacgaagct gagcggcgtt
     3241 cgctttcaca caagcaaatg ctgagacttt atctaacccg attaaaaatt cgaagtcgtc
     3301 ttcgacctga tgaatatcga atttattgtc cttgttatta ctaactattt cctgagggtc
     3361 tttttgacat cccgtcaggc cgagcgtgag cgaaaagaga actagtatta atgcacgcca
     3421 acgcacgatc ggtttgctag gaaccataga attctcctat tttgtagttc taaataggat
     3481 cgctcattaa tggcttaata cccatagcgg cagaggattt ccgggcttac gacttgcgct
     3541 ttagcgctgg actcgtgact aaaggcccaa tcttaattga aatttaatag cgaggtcagg
     3601 gcctgaaaca tcccggacag gaccaaaata tcaatccacc tctttcctcc tgaccactac
     3661 tcagtgaaaa ataaaaacta tttactcccc accggatctc ttcccttgcg actgagtgtg
     3721 gtccaaccct cttctccctt ggcggggaat gagagccttg atttcattaa atgctcgatc
     3781 tgcagattga gcatctttaa aaaacaaaca aacctctttc tcgcccagga tccggccagc
     3841 gaaaagaagg attcaattac gaagtaaagc ttgagtccgc ttaaagcgtt tagcgggaga
     3901 aagagtttca tattcgcaat tcatgtccgt cagaaggcag tggtttactg actatcggta
     3961 ttcatgcgcg ccaatatcga tacctgctcc aacaggtcta ctcattccta aaaaatcgag
     4021 gagaggcggg tttacgctgg atccagcatc aatcgcaata cttccaagct taagtcgata
     4081 atccaaagat acagggtttg cgaaaatctg acttaagttt cccgcgtttg atacaagatt
     4141 gtggtctgcc gttgaacccg aacctgggtc aatggtcggc gcaatattat ttcgtattat
     4201 ggagttttgc gatgaagttc catttttatg agaagtgatc ataatccatg gacgaatgtt
     4261 gtccgttaaa ttggggtcaa tcactgtgtt attggcaatt ttcacattaa tagcaccgta
     4321 aaaagaaatt gcatgccaat gatttactac aacaacatta ttttccacca cccaatcctt
     4381 aaacattcca tcaaacaatc ctatcgcctg taagttacaa gtgacgatgg gattaggagt
     4441 atctgattcc aaaaaataat tgccacgaag aacaattccc gtaaccacac ctgagcctgg
     4501 gccaccagga ccatttgacc acgactgaaa cccgtccata tggttatcat caacttgcct
     4561 gcaattcctc acatcattgt actcaaacac tgtaccgtct ccaagaccac gcattccatc
     4621 cccacgaaaa ccatcaattt tatttcgtga aatcagagag tttgttgcgt ctacagaaag
     4681 tgcaaatgca atatttttta tgacgttttc tcgaacggta atgcgtgttc cagaaactcc
     4741 cactccatca cgtgcatgat tgagccaatt gcttgccacc catgacgaag agtcatcaat
     4801 gtaattgacc atagattttt caaaaacgat atcttgtcca gaaatcgaaa ccgccgtact
     4861 gacattgtca ccactcgcaa ctctcggaag caccgacact ccgctaattt gaataaagcg
     4921 gctgttctga atatgaatac ttttaaaaac cggactggcg ttttgctgtg gtcgaatcac
     4981 aaccattgaa gggtacatcc tgccattgat ggtgtattga ccataatctc cggaggccaa
     5041 ggcaatggtt tcaccaccaa cggcgctttg caaagctgcc gtcaactctg ttgcatttga
     5101 caccatgatt gcatttggcg gaatcggagt gagatcctgt ggaggtgcat ctgctacaca
     5161 atttggcccc ggacaaccat cttgcgattg attatcagaa cttccctttt cagactggta
     5221 gcttccgcca ccgcaaccca ctaaacaagc aatcacggct aaaaatgaga cagttcttaa
     5281 aatcataatg tagcccccac ctgcctttca tattcagcaa gaaacaagcc gcggtgaaaa
     5341 cctaaaaact cattggtttc gccttgttgc ctcaaaatga aatgaattac cgggcgactg
     5401 tctaaagaag cgattgtgaa tagaatttcg acaacgcgaa gctgaaggta aaacttagaa
     5461 gccacgatcc atcagatcgt ggcctctttt attagagcaa gattcaaaga tttaaccctc
     5521 acctaagggc ttcacaaaat cgtaatactt gccgcttttt atatcgtagc tctgcttttt
     5581 acctttttca cgggtaagag aaaatagctt ttcgagttta gaaacggaat agatgggata
     5641 cctacccacc ctatatgatt gtcctgatga aaaggtcagt gtgatttctg tgcgcgccgg
     5701 agaagcacca gaactctctt tttccgcgtc ccacatttc
//
LOCUS       DAORQN010000087         5719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_598_length_5719_cov_14.841984, whole genome shotgun sequence.
ACCESSION   DAORQN010000087 DAORQN010000000
VERSION     DAORQN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_5719_cov_14.841984"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK614_06100"
     CDS             <1..398
                     /locus_tag="PK614_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81819.1"
                     /translation="DKKVVWLLLSRMKHFKTVPYQAEQDLKVSERLEGEVSREEIGTE
                     TREGHPTTLYEVTVKEGERTEVYYQWLATDIRFPMKLAKKDGSWIVEYQHVKIRPVID
                     YLFQLPLNFEPLEEFDHQPAAADSSQQPM"
     gene            429..1355
                     /locus_tag="PK614_06105"
     CDS             429..1355
                     /locus_tag="PK614_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB81820.1"
                     /translation="MRKAGMTVWALSVAVLGMTASLYALDVADVVREWTPEGKKLAME
                     RAKLPAHDEMIRIPAGEFLMGSDRKVDKNSYLAEFPQRKIYLDAYEIDKHEVTTVQFL
                     KFVLASNRDPLIDWQYDGGNFQDTMVSHPVMHVSWFDAEAYCKWAGKRLPTEAEWEKA
                     ARGEDGRIYPWGNQMAGLSRANFGRTGLSGPVRDRPERLLLYPPIISVDKYDNAASPY
                     GLFQMAGNVAEWVADWYDPKYYTTGPDKNPKGPETGSQRSFRGGGWIDSTPSVRVAQR
                     NGTDPNTKMNWMGFRCARDVNEGASGQPTEAK"
     gene            1362..1886
                     /locus_tag="PK614_06110"
     CDS             1362..1886
                     /locus_tag="PK614_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81821.1"
                     /translation="MPHLAIPTQRRTNQLVSGLMSLVLLLAACQIEDLNIEILNEDGS
                     VLFIVHENDAPDPTPLSRFGVVSHYSGARWEVATYEESSLYRMQDGKKLLKPLDELPE
                     INALAVGAIRFGEVPSGFFIQGSSGNHLVALEEGRVYEAYANRGAQRGHISFTIEHGV
                     AVKLPPVTHPLSSP"
     gene            complement(1861..2703)
                     /locus_tag="PK614_06115"
     CDS             complement(1861..2703)
                     /locus_tag="PK614_06115"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRB81822.1"
                     /translation="MAGAGFNGLTVAAFESRMAAEMTRLIERHGGKPLVAPALREIPL
                     EDNSAALQFGELLLTEGLDVLVLLTGAGTTTLFEILHSRHAKDTIKNALKETVLIARG
                     PKPVAALKALGFQPTLTVPEPNTWVDVVSTLDAYRPVTGLRVAVQEYGLPNRDLVEAL
                     KQRGANVVSVPVYRWALPEDTTPLKRVLNEILAGQVQVILVTNAAQIDHVMQVLEQEG
                     NTAQFKTICRQLVVASIGPTASERIRSHGLTVDFEPSHGKMGILVKETSEQAHSLMAR
                     KAGA"
     gene            complement(2745..3584)
                     /locus_tag="PK614_06120"
     CDS             complement(2745..3584)
                     /locus_tag="PK614_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB81823.1"
                     /translation="MVARSVLAIVVALIGLNGVSLHAFAAQQAALPKEITGKDGAPLV
                     LIPAGSYPMGVPTGDRDGGRDEYPRHVIEITDFYIDKYEVTNGRYLEFVKATNHRVPQ
                     NPKNPTRNLWEGIGIPASLADRPVINVDWADADAYCKWAGRRLPREAEWEKAAKGNND
                     WRFPWGNVEPTDKHLNFNQKWIGEKTLMPVGSYESGKSPYGVYDMAGNVWEWVNDWYD
                     AKYYEKSPDKNPPGPASGDKRVIRGAGWQNETPTVRIFTRVDSDPTIRNESTGFRCAM
                     DAR"
     gene            3758..4189
                     /locus_tag="PK614_06125"
     CDS             3758..4189
                     /locus_tag="PK614_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB81824.1"
                     /translation="MPTHRGLRHLALRVTDLARSRAFYERFLAMKVVWEPDPDNVYFS
                     SGSDNLALHQIAPAELAQYQPVRGQLLDHVGVIMESPAHVDDLFRDVQQDGARYGATI
                     AKPPKQHRDGSYSFYFADPDGNVIQALYEPTISRMEFRPLA"
     gene            4201..>5719
                     /locus_tag="PK614_06130"
     CDS             4201..>5719
                     /locus_tag="PK614_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB81825.1"
                     /translation="MWDTLARDQYVGVAPWVWVQLESNQPPGPFPFIGGVAPEVVASL
                     HEAHSLFLACVETAISDVFSRRATLGDPQTRVRLEDAYAELVNSRQQLSQHITARRQP
                     DGQFHWSHPFDATKSSTVVNTGLRIFNAVKRQAIPVPFERPMGPVVGKLLGMLDGTQQ
                     AGAIQTIVATAGRDGERLLTKLMELLVQYECLTPTKHASIRDQWLANTKDRDTIHLGH
                     AALMYRQQDRFLLFDPWLLPWFAESNVPSLWVSMLPKPAAIFLTHDHDDHVDPRTLLH
                     VPKDVPIVIPSRRNRKKFFYDYLPLLRELGFTHIVELAHGETWNFDGGAVVSVPFYGE
                     DPCDLEMPRNCYLVTDRGQNVLVHADSGPTNNGRSAIKEGVIQQLVQKYGPISLVLAS
                     QQQLQEIRSYAAHAPLSHPGLWLEVGENGYLTNGYLDELCAVAQAKLFVSYATGGADW
                     YPDHLSFMFSQRNPARTALLTANWEQPEKLKDLLGARGCGYHRAHALDLFRSSGDG"
BASE COUNT         1140 a         1627 c         1755 g         1197 t
ORIGIN      
        1 aagataagaa ggtggtctgg ctcctgttgt cgcgtatgaa gcacttcaag accgtgccgt
       61 accaggcgga gcaggatctg aaagtgtcgg agcggctgga gggggaggtg tcgcgcgaag
      121 agatcgggac ggaaacgcgc gaggggcatc cgacgacgct ctacgaagtg acggtgaagg
      181 aaggcgagcg gacggaagtc tactatcaat ggctcgcaac cgacatccgt tttccgatga
      241 agctggccaa gaaagacggg agctggattg ttgagtatca acacgtgaag atcagacccg
      301 tgatcgacta tctgtttcag ttgccgctca atttcgagcc attagaagaa ttcgatcacc
      361 agccggctgc cgcagactcc agtcagcagc cgatgtaagc gatgatcggg aaataaagga
      421 gcagcattgt gcgtaaggcc ggaatgactg tatgggctct cagtgtagcg gtattaggaa
      481 tgactgcttc cctctacgcg ttggatgttg ccgatgtcgt tcgtgaatgg acgccggaag
      541 gcaagaagct ggcgatggag cgcgccaagt tgccggcgca tgacgagatg atccgtatcc
      601 cggcgggcga gttcctcatg ggaagcgata gaaaagtcga caagaattct tacctggccg
      661 aatttcctca acggaagatc tatctcgacg cctacgagat cgataagcac gaggtcacaa
      721 ccgtgcagtt cttgaagttt gtcctggcgt cgaaccgcga ccccctgatc gattggcagt
      781 atgacggtgg gaattttcag gacaccatgg tgagccatcc ggtcatgcat gtgtcctggt
      841 tcgacgcgga ggcctattgt aagtgggcgg ggaaacggtt gccgacggag gctgaatggg
      901 aaaaggccgc gcgtggtgag gatgggcgca tctatccctg gggcaatcag atggccggac
      961 tctccagggc gaattttggc cgcacgggcc tgtcaggacc ggtgcgagac cgccccgagc
     1021 ggctgctgct ctatccgcct atcatttctg tcgacaagta tgacaatgcc gccagcccct
     1081 atggtctgtt tcagatggcg ggcaatgtgg cggagtgggt tgcagactgg tacgacccga
     1141 aatactacac caccggaccg gataagaatc caaaaggtcc ggagaccggc agccaacgga
     1201 gctttcgggg tgggggctgg atcgatagca ctcccagcgt gcgggtggcg caacggaacg
     1261 ggacggaccc caacacgaaa atgaactgga tggggttccg ctgtgcacgg gatgtgaacg
     1321 aaggggcctc aggacagccg accgaagcga agtagtcgga ggtgcctcac ctggcaattc
     1381 ccacacagcg aaggaccaat cagctcgtct caggcctgat gagccttgtt ctgctgctcg
     1441 cggcctgtca gattgaggat ctcaacatcg agattctgaa tgaagacgga tcagttctat
     1501 tcatcgttca cgaaaacgat gcaccggatc ccacacctct aagccggttc ggcgtcgtct
     1561 ctcactactc cggcgcgaga tgggaggttg cgacctacga agagtcatcg ctgtacagga
     1621 tgcaagacgg caagaagctg ctcaaaccgc tggatgagtt gccggagatc aatgctctcg
     1681 cagtgggagc gattcggttc ggggaggtgc cgagcgggtt tttcatccag ggctcctcag
     1741 gcaatcacct cgtcgcgttg gaggaaggca gagtctacga agcctatgcc aaccgaggcg
     1801 cacagcgagg gcacatatca ttcacgattg agcatggggt agccgtcaag cttcctcccg
     1861 tcacgcaccc gctttcctcg ccataagcga gtgggcctgt tcgctcgtct ctttcaccag
     1921 aatccccatc tttccgtgtg agggctcgaa gtccacggtc aggccgtggc tgcggatgcg
     1981 ttcgctggcg gtgggaccaa tcgaggcgac gaccagttgc cggcagatcg ttttgaactg
     2041 cgctgtgttt ccttcctgtt ccaacacctg catcacgtga tcgatctggg cggcattggt
     2101 caccagtatc acctgcactt gtccggctag gatttcattc agtactcgct tcagtggagt
     2161 ggtgtcttcc gggagtgccc agcggtagac ggggactgaa accacattgg ccccgcgctg
     2221 cttcaacgct tccaccaagt cgcggttcgg taacccgtat tcttgcaccg ccacgcgtaa
     2281 ccctgtgacc ggtcggtagg catccagtgt cgagacgaca tccacccagg tgttgggctc
     2341 cgggaccgtc aacgtcggtt ggaatcccag agctttgagt gcggccaccg gtttggggcc
     2401 gcgcgcaatg agcaccgtct cttttaaagc attcttgatc gtgtctttgg cgtgacggct
     2461 gtgcaggatt tcgaagaggg tggtcgtgcc ggccccggtc agcaggacta gcacgtcgag
     2521 gccttcagtc agcaacagct cgccgaattg cagcgcggca gagttgtctt ccaacggaat
     2581 ttcccgcaag gccggcgcca ccagcggctt gccgccgtgg cgctcgatca gccgcgtcat
     2641 ttccgccgcc atgcgcgatt caaatgcggc gacggtcaac ccgttaaacc ctgcaccggc
     2701 catcgtactc cgtgattcaa gcaccggtta ccatgccgac tgagttacct ggcgtccatc
     2761 gcacatcgaa acccggttga ttcgtttcga atggtcggat cgctgtccac acgggtaaag
     2821 atacgaaccg tgggagtctc attctgccac ccggcgcctc gaatgacccg cttgtcgccg
     2881 ctggccggtc ccgggggatt cttgtcgggg ctcttttcgt agtacttcgc atcgtaccag
     2941 tcgttgaccc attcccagac attgccggcc atgtcataga caccgtaggg actctttccg
     3001 ctctcgtagc tgcccaccgg cataagcgtt ttttcgccga tccacttctg gttgaagttg
     3061 aggtgtttgt cggtcggttc cacattgccc caggggaagc gccagtcgtt gttgcccttg
     3121 gcggccttct cccattcggc ctcccgcggc aatcgcctgc cggcccactt gcagtaggca
     3181 tcggcatcgg cccaatcgac gttgatcacc ggacggtcgg ccagggatgc gggaatgccg
     3241 atcccttccc agaggttacg ggtcgggttc ttgggattct gcggaacacg gtggttcgtg
     3301 gccttcacga attccaggta gcgtccgttc gtcacttcat atttgtcgat atagaaatcg
     3361 gtgatctcga tgacgtggcg ggggtattca tcgcgtccgc cgtcgcggtc tccggtcggc
     3421 acgcccatcg ggtacgagcc tgccggaatc aggaccaacg gcgcgccatc cttccccgtg
     3481 atctctttcg ggagagccgc ctgttgcgct gcgaatgcgt gcaaactcac accgttcagt
     3541 ccgatgagcg ccaccactat tgcgaggacg gatcgtgcca ccatagctca acactccttt
     3601 ggttcgaaaa agacgatggc agatcgtacc acagcggcgg agaattcaga cacggcttgg
     3661 gcagagaatg tgcgcactcg gggtgggccg atgcaggaga atgaggattg acggggtgac
     3721 aggcccccac ctactatacg gccaaggaga ttccgacgtg ccgacccatc gaggcttgcg
     3781 acatctggcc ctccgtgtga ccgatcttgc ccggtcgcgc gccttctatg agcggttcct
     3841 ggccatgaaa gtggtgtggg aacccgatcc ggacaatgtg tatttcagtt cggggtccga
     3901 caacctggcc ctgcatcaga tcgcgcctgc cgagctggct cagtaccagc cggtgcgtgg
     3961 acagttgctc gatcatgtcg gtgtgattat ggaaagcccg gcgcatgtag atgacctgtt
     4021 ccgggacgtc caacaggatg gggcacgcta cggggccacc atcgccaagc cacccaagca
     4081 acatcgcgac ggcagctact ccttctactt tgccgacccg gatggaaacg tcattcaggc
     4141 cttatatgaa ccgaccatca gccgcatgga gttccggcct ctcgcctgac gagcatgggt
     4201 atgtgggata cattggcgcg cgatcagtat gtcggggtgg ctccctgggt gtgggtgcag
     4261 cttgagagta atcagccgcc ggggccgttt ccgttcatcg gcggggttgc cccggaggtg
     4321 gtggcgagtc tgcacgaagc tcacagtctc tttcttgcct gtgtggaaac cgccatcagc
     4381 gatgtgttct cccgccgcgc gacacttggc gacccgcaga ctagggtgcg gctggaagac
     4441 gcctatgccg agctggtcaa ttcccgacaa cagctgagtc agcacatcac ggcccggcgt
     4501 cagccggatg ggcagtttca ctggtcgcat cccttcgatg cgacgaaatc ctcgaccgtg
     4561 gtcaacaccg gcctgcgaat cttcaatgcc gtgaaacgcc aggcgattcc tgtgccgttc
     4621 gagcgtccca tgggaccggt agtaggaaaa ctgctgggga tgttggatgg cacacagcag
     4681 gccggggcga ttcagacgat cgtggcgacg gccggtcggg acggcgagcg gctgttgacc
     4741 aagttgatgg agctgttggt ccaatacgaa tgcctcacgc cgacgaagca cgcctcgatt
     4801 cgagaccagt ggttggccaa taccaaggat cgggacacga ttcatctggg ccatgccgcg
     4861 ttgatgtacc ggcagcagga ccggttcctg ttgtttgatc cgtggctgtt gccctggttc
     4921 gcagagtcga atgtgccgag cctctgggtc tcgatgttgc cgaaaccagc cgcgatcttt
     4981 ttgacccatg accacgatga ccacgtggat ccccggaccc tgctgcatgt gccgaaagac
     5041 gtgccgatcg tgattcccag ccggcgcaat aggaagaagt ttttctacga ctacctaccg
     5101 ctgcttcggg agctgggatt tactcacatc gtcgaactgg cgcacgggga gacttggaac
     5161 ttcgacggcg gcgccgtggt ctccgtcccg ttttacggcg aagatccctg cgatttggag
     5221 atgccgagaa actgctacct cgtgaccgat cgcgggcaga atgtgttggt gcatgcggac
     5281 agcggcccga ccaacaacgg ccggtcggcc atcaaggaag gggtgattca gcagctcgtc
     5341 cagaaatatg gtcccatctc gttggttctg gcctctcaac aacagctgca ggaaatccgt
     5401 agttatgccg cccatgcgcc cttgtcccac cctggtcttt ggctcgaagt cggggagaac
     5461 gggtacctca cgaacgggta tctggacgag ctctgtgccg tcgcccaagc gaagctgttt
     5521 gtgtcctatg ccaccggcgg tgccgactgg tatcccgatc acttgtcgtt tatgttcagc
     5581 cagcgcaatc cggcacgcac ggcacttctc acggcgaact gggagcagcc ggaaaaattg
     5641 aaagatcttc tcggagcgcg agggtgcggg tatcatcgcg ctcatgcgtt ggatctgttc
     5701 cggagcagcg gtgacggga
//
LOCUS       DAORQN010000088         5665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_602_length_5665_cov_8.176827, whole genome shotgun sequence.
ACCESSION   DAORQN010000088 DAORQN010000000
VERSION     DAORQN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_5665_cov_8.176827"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..790
                     /locus_tag="PK614_06135"
     CDS             149..790
                     /locus_tag="PK614_06135"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HRB81826.1"
                     /translation="MPAYVGSLALIVSVAFLSWSLGGRQETPPERASFVDFAMQIGQW
                     RGKSLPVEPQFISALRFDDYLLADYALAGGDPITLYLAYYQSQREGQSAHSPQSCIPG
                     GGWEITSLRTVNLSVDGSNGLTVPVNRVMIQKDRQKLIVFYWFKQRDRYLSSEYLVKF
                     YLFWDALMKQRSDGALIRLSAAIEPNESEQAAENRLLDFARSIQPQLSRYIPD"
     gene            complement(1034..2254)
                     /locus_tag="PK614_06140"
     CDS             complement(1034..2254)
                     /locus_tag="PK614_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRB81827.1"
                     /translation="MTLCNILNNAVTFVTSAAIASLVSAEEFGIFSVSVNVTMIVFAV
                     SEAGLSLSLVRHYSHEKNGEARRNILRTGAVLRLAIALALLILSIPLGWALSLVLSPN
                     HPISRELTIGVMAAGALGLWASARATQQATQNYKKYAQLTFLYGLTRFLIVGIFYLAG
                     TREAVLYLAALYMVSPLITAVGFYRTFHSQYRLELASLRFPVIKSLFAYGRWVLASTF
                     LSPMCYTLPLFLLMSLRGAGDAASYGVGLMFSAVVGPLGDAIGIYLVPKVTSFSNPQE
                     VREYIRHILKFMGPFLALVAIAISIVSVIYGILYTHKYPDGLLTTQLLLASNFLASYG
                     GIMNCVTHYFGTPHLNMLANLGKIVICGGVAWVLIPHYGAEGAAFAAAIAAVIGETGL
                     FITMQCRLSKLSLR"
     gene            complement(2618..4927)
                     /locus_tag="PK614_06145"
     CDS             complement(2618..4927)
                     /locus_tag="PK614_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81828.1"
                     /translation="MTIRAKTSLNKVPKWIGFSSINLHSQNSRPFPKMQLALVFAIFV
                     LASPPLQCSYAKPPESKVQILSLTPAPNYPRTTDSLDIQQLTDGILETHPMWIRKGGI
                     GWARLTPIVIKMKVADPGVGTESVSGILRLHTGKQLASGVTPPKRIDVYTQDQSGGYS
                     RVAGLQLQTVTFSDKSSHWIELPIIGAGRVFLVIVHADNDYFFLDEIQWVPTANKPGN
                     RASATLATPEKVIADSLGNLKQSFLSAATPPPTPKSVATEKGVASIMLWAQAPWDKFD
                     SSPNRQEVLRRKDQPILLLGTKDELESGCLGIINTSSDSKKIALKIAQRSDRANSIQI
                     RVVREVMAANGNLVFDALTPLGDEDNILLPPGVAVYFWITADLSKYCCGKTELAVQVT
                     DVKADVALQLPLQIVVADHSLSSKRVRAVNWAYISDKPIWSNPAAVVNDLATHGVNVF
                     VIPPWILPQPTLNGGWDVYSARRFEETVTLFRGRGMLLLYMGLNASNDSSNKKLTWLY
                     SSTSKDSLARKNAMRSWLTTLTSELARLGFNKNDWALYPVDEARGEDLDRLQRIATLI
                     KEIDPNVQLYANPDSTSDTSITREHLEALKPLIDIWQPNLLLASSTGKDFFQNLHAPW
                     WIYSNSSTPAKAASPLNHYHAMSLKAWALGAEGVGFWSYSDTAGTSAWDDLDGARPDW
                     AVVYENDIPSSSRRWEAFREGLEDHRLLQLAVSDNPLLSPSEKESWKNEIRILTSSPL
                     TTRQLQRIRLQLLRLIQTNLGLDIDLDSP"
BASE COUNT         1390 a         1420 c         1402 g         1453 t
ORIGIN      
        1 gatactcctg gaaatgtggg gtctatctcg cgtgcagcct ttccctggat cagagggtct
       61 tttaaagcgg ttcacgctgc acgaggagcc agaaccggct gtttgtgctc cacaagtcgt
      121 ccgcccgact ccggccacga tgccaatcat gccagcgtac gttgggagcc tggcccttat
      181 cgtttccgtc gccttcttgt cttggtcgct tggcggcaga caagaaactc caccggagcg
      241 tgcctcattc gtagattttg ccatgcaaat tggacagtgg aggggcaaat ctttaccggt
      301 ggagccgcaa ttcatttctg ccctgcggtt cgacgattac ctgttggctg actatgcgct
      361 agcaggtgga gaccctatca cactctactt agcctattac cagtcccaaa gagaggggca
      421 atccgcacat tccccgcaaa gttgcatccc tggaggaggg tgggagatca cctctcttcg
      481 taccgtgaac ttgtcggtag atggctcgaa tggactcacc gtgccagtaa atcgtgtcat
      541 gattcagaag gaccgccaaa aactgattgt gttctattgg ttcaagcaac gcgatcgtta
      601 tctttccagc gaatatctcg tcaaatttta cttattctgg gacgctttga tgaaacagag
      661 gagcgatgga gccttgattc gcctctcggc cgccattgaa cccaacgaga gcgaacaggc
      721 agccgagaat cgactgctcg attttgccag atccattcag cctcaactta gccgttatat
      781 cccggattga gtgagccgaa aagcaggagc acgattggct gtacaccgga ccaccttcct
      841 ctaaacagga tgcacagcga aaccgtgagg ccaacataca gggggagatg tgccgctgtt
      901 tctccgccaa actctaccag taacccatgg gtgatcatta tgattagcca cattcctgcg
      961 gcatgcaccg gtagcagtat ggccagtatg aagaaaaaac ctagacacaa gatgatcgag
     1021 aacattgatt tgcctatcta agcgacagct tactcaatcg acactgcatg gtgataaaga
     1081 gaccagtttc gcctatcaca gcagcaatag cggctgcgaa ggcggccccc tccgcgccgt
     1141 aatggggaat tagtacccat gctactcctc cgcaaattac tatcttccca agattcgcca
     1201 acatattaag atgaggagtt ccaaagtagt gtgtgacgca attcatgatg cctccatagc
     1261 tagctaagaa atttgacgcc aggagcaatt gggttgttag gagaccgtca gggtatttat
     1321 gcgtgtacaa aatgccgtat atcactgaca ctatgctgat cgcgatggcc actagggcaa
     1381 gaaatggtcc catgaatttg agtatgtgtc taatatactc tctcacttct tgaggattag
     1441 agaaggaggt gaccttcggt acgagataaa tcccaatggc atcccccaat ggtcccacga
     1501 cagcgctgaa catgagaccc accccgtatg acgctgcatc accagctcca cgtaaactca
     1561 taagaagaaa aagaggaagg gtgtaacaca tgggactcag aaaagtggag gcaagtaccc
     1621 accgtccata agcaaacagg ctcttaataa caggaaatct caatgaggcc agttccaagc
     1681 ggtattggct atgaaaggtg cgataaaaac cgacggctgt gatgagcggg cttaccatat
     1741 agagagccgc aaggtacaga accgcctcgc gcgtaccggc caaatagaat atcccaacga
     1801 taagaaagcg cgtcagccca taaaggaaag ttagttgggc atatttttta tagttctgag
     1861 tcgcttgttg ggtagcccta gctgatgccc agagtccaag ggctccagcc gccataacgc
     1921 caatcgttaa ttctctgctg attggatgat ttggtgagag gacgagcgac aaggcccagc
     1981 ccagagggat tgaaagtatt agcaaagcta acgcgatagc gaggcgtagc acagctcctg
     2041 ttcgcaggat gttacgtcgt gcttcgccat ttttctcatg gctataatgt ctgacgaggg
     2101 agaggctgag ccccgcctca ctgacggcga atactatcat tgtgacattt accgagactg
     2161 agaatatccc aaattcttca gcgctcacta aggaggctat ggcggcgctt gtaacgaaag
     2221 tgaccgcatt gtttagtata ttgcacagcg tcataagccc tacatccccc gatgtagacg
     2281 atcgtagacg gcgcgtatag atttggatgt ttttttgtag gggtctgtaa agatgaagtg
     2341 tcgtcatcat ttcttgtcag ttaatagatt cgctcttccg tttgcagaag ggcaatcaag
     2401 attttgttcc ctgccctgac ctactcgtct tgtttcttca attgcgtgtt accagcccca
     2461 gactctccca ttggaatgtc caagggggtg ttgtctaggc ccaggccgga agcattataa
     2521 gccgcgttcc tagagaaccg accgcgcccg ctaacgatta tgataatggc ggcaagatca
     2581 ctgtgttcga tcctggcatg agctgcgcga gctttgatta gggggagtct aagtcgatgt
     2641 ctaaccccaa gttagtttgt atcagcctca ggagttggag gcgaattcgc tgcaattgtc
     2701 tggtggtaag tggactagac gtcaagatgc gtatttcatt tttccaggat tctttctctg
     2761 acggagacaa gagagggtta tcacttactg ctaattgtag aagtcgatgg tcttccaaac
     2821 cctcccgaaa tgcctcccac cgtcggctac tgctggggat atcgttctca taaaccacag
     2881 cccaatcggg ccgtgcgcca tccagatcat cccatgcgga tgttccagcg gtgtcgctat
     2941 aagaccaaaa tcctaccccc tctgctccca gggcccatgc tttcaaggac atcgcatgat
     3001 aatgattgag aggcgatgca gcttttgcag gagtcgaaga gttgctataa atccaccacg
     3061 gagcatgaag attctgaaaa aaatcttttc cggttgaaga cgcgagtagc aaattcggct
     3121 gccaaatatc aatgaggggt ttcagtgcct cgagatgctc tcgagtgatt gatgtgtcag
     3181 acgtgctgtc cgggttagca taaagttgta cgtttggatc tatttctttg atcaaagtag
     3241 caatgcgttg aagtcgatct aggtcctcgc cgcgtgcttc atccacaggg taaagagccc
     3301 aatcattttt attgaagcct agtcttgcga gctcagaggt gagagtggtg agccagctac
     3361 gcatagcatt cttgcgggcc aaactatctt tgctagtact gctgtacaac caggtcagtt
     3421 tcttattcga agagtcgttg ctagcattca gacccatata cagtagcaac attccccgcc
     3481 ctcggaacag tgtgaccgtc tcctcaaatc tccgcgcgct atacacgtcc cagccaccgt
     3541 tcaaagttgg ttgcgggagt atccatgggg gtatgacaaa cacgttcaca ccatgtgtag
     3601 caagatcgtt gaccaccgca gcagggtttg accaaatagg tttgtcgctg atatatgccc
     3661 aatttactgc ccgcacccgt ttactcgata gtgaatggtc tgccacaaca atttgaagtg
     3721 gtagttgaag agctacatct gccttcacat cagtcacttg tacggccagc tctgtcttac
     3781 cgcaacaata tttgctgagg tcggccgtaa tccaaaaata cactgccact cctggcggga
     3841 gcagtatatt gtcctcatcg cccaacggcg ttaaggcatc gaaaaccaaa ttcccattgg
     3901 cagccatgac ttccctgacc acccgaattt gtatgctgtt cgcgcgatca cttctttggg
     3961 ctatttttaa ggcaatcttc ttcgaatcag aggaggtatt tatgatacct aggcaaccag
     4021 actccaattc atctttcgtt cctaagagca aaatcggctg gtcttttcga cgcaacactt
     4081 cttgtctatt gggggatgaa tcaaacttgt cccagggagc ctgggcccat aacatgatag
     4141 aggctacacc cttttccgtt gctacgcttt taggagtggg aggaggtgtc gctgcagata
     4201 aaaaagattg ttttaggttg cccagactat cagcaatgac tttctctgga gtagcgagtg
     4261 tggcactggc acgattcccc ggcttgttcg ccgtagggac ccattgaatt tcgtccaaga
     4321 agaagtagtc gttatccgca tgcacaataa ccaagaaaac acgtccggcg ccaattatcg
     4381 gtaactctat ccaatgactt gacttgtcag aaaatgtgac agtttgcagc tgcagccctg
     4441 cgacgcgact gtatccgcca gactggtcct gcgtatacac gtcgatccgc tttggagggg
     4501 ttaccccaga tgctagctgc ttgccggtgt gaagacgtaa tatgccagaa acggattccg
     4561 tgcccactcc aggatcggca actttcatct tgataacaat gggagtcaat ctggcccaac
     4621 caattccgcc tttcctaatc cacatgggat gcgtttcgag aatcccatcg gttagttgtt
     4681 gaatgtcgag tgaatcagtt gttcgtggat agttcggggc tggcgtcaag cttaggatct
     4741 gaactttact ttctggtggt ttggcataac tacattgcag tggtggcgat gccaatacaa
     4801 aaatggcaaa cactaaagct agttgcattt ttggaaaggg cctagagttc tggctgtgca
     4861 ggttaattga tgagaaccca atccatttag gcactttatt caaactagtt ttagctcgta
     4921 tcgtcatact accttctgta ttgtgtgaac atgttgaata gatccatatt ttctagaccg
     4981 atgtgatttt gatagattgt cctgcgtagt gtatcccgta gtcagtatga tcgtgccgtg
     5041 tagcttggaa ataccgtagt gtgttgaccc tgcgggttca tgaaactaag ctgttgggga
     5101 tactgcgggc catgtggctt ttaaccaata agaattctcc caaacattgc cccatctacc
     5161 agctgacacc ggtcttggtt gcttctcccc tatccgtgcc tgactctatt ttggcgaggc
     5221 ttcatcggat gtattcatcc atgaaagacc gcgaccaacg tttcccttat cacaattccg
     5281 ctacgtagtg ttagtgccgt tcgccactga ctccagatcc atacagacaa tgtctcagca
     5341 tgacacactc gtgcggggtt gcgcgtctga agtgtgtcag gaatgacggc cgatcggtac
     5401 gtggtatttc ggcattgacc tcagcgcctg acgaatgtac tgtgccttta gcgactggca
     5461 tcttgccaag gccgaatgga cggagccttg ttcacacgtt ttcttcagac tcactctgta
     5521 tggctgatcc ttgcgcgtaa cgctgtcatt caattccaat gccctccccc aacgcctcgc
     5581 ctcgtcgctg aaggcgctcg ctgcgccagg acgtttggag tggtggagcc acgcgaggga
     5641 acgcgcgctg ttttcgttgc tgccc
//
LOCUS       DAORQN010000089         5652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_603_length_5652_cov_8.764338, whole genome shotgun sequence.
ACCESSION   DAORQN010000089 DAORQN010000000
VERSION     DAORQN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_603_length_5652_cov_8.764338"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1448
                     /gene="hisD"
                     /locus_tag="PK614_06150"
     CDS             165..1448
                     /gene="hisD"
                     /locus_tag="PK614_06150"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRB81829.1"
                     /translation="MTILASTDRAYDKALRKIVTRARTQGAGVEKSVRTILKAVERGG
                     DRAVLRYTKKFDRLSLKLDHMRVTPEEIKEAYYHIRKDEGDSLRYAAERVRQFHERQR
                     TKTWMYQEQNATLGQMVTPLDAVGVYVPGGKAVYPSSVLMCAIPAKVAGVRRIVMCTP
                     TPKGEINPYLLVAADIAGVDEIYRVGGVQAVGAMAFGTKTIAKVDKIVGPGNIFVATA
                     KRLLYGTVGIDMVAGPSELLVVADDDAKPAHVAADLLCEAEHDEDAQVYLVTTSASLA
                     KEVVRLIQVQLKGLQREKIAAKSIARHFAAFVVPTMDEAIEVANAIAPEHLTLSVDRP
                     FDYLEQVRHAGALFLGRYTPPAVADYVAGPNHVLPTGATARFFSPLSVNDYVKVSNIV
                     HYTKEELTKAKDHIVRLAHIEGFDAHAKSAQSRFA"
     gene            1445..2050
                     /gene="hisB"
                     /locus_tag="PK614_06155"
     CDS             1445..2050
                     /gene="hisB"
                     /locus_tag="PK614_06155"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRB81830.1"
                     /translation="MKKNGAPRQASLHRATKETDIRVEWTLDGTGQGTVDTSIRFFDH
                     MLDLLAKHGFFDLTVQAKGDIEIDEHHTVEDVGIVMGKVLHQALGEKAGIKRFGFASV
                     PLDETLAQVTVDLSGRPYLVYNVNLPDRKIKTFDLGLFEDFFQAFVTHGGLNLHVNLQ
                     YGRNPHHIMEAIFKALARALDQATTLEERLSGAVLSTKGSL"
     gene            2137..2745
                     /gene="hisH"
                     /locus_tag="PK614_06160"
     CDS             2137..2745
                     /gene="hisH"
                     /locus_tag="PK614_06160"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRB81831.1"
                     /translation="MIAIIDYGMGNLRSVSKAFEAMGHQAVVTREARVIADASHVVLP
                     GVGAFGDCMANLERYELIAPIHAAVQSGKPFLGICLGLQLLFTESEEFGVHAGLGIIP
                     GRVKKFVLEPPLKVPHMGWNEIQIVQSAPPFAGIATGSYCYFVHSYYVEPVESSVIAT
                     VTEYGRSFASAVWKDNVVACQFHPEKSQAVGLQVIKNFGAWT"
     gene            2778..>3198
                     /locus_tag="PK614_06165"
     CDS             2778..>3198
                     /locus_tag="PK614_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81832.1"
                     /translation="MTLLLLAGCSGTKVTSKASSQVGKYPVRTIALIPFETMATPQAI
                     EASGPAFSVPSGARRSDMAVGVPPTTDQAVRQTHRVPASAGEKVTDLMWAKLKARSGL
                     QLLSPNESARVAREVSGGTATETMPAHKIAQRLGADAA"
     assembly_gap    3199..3208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3209..3469
                     /locus_tag="PK614_06170"
     CDS             <3209..3469
                     /locus_tag="PK614_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81833.1"
                     /translation="VYQERVGSRLGADPPAAVGFEVKLVAPDGIVLWEGNYYEKQRPM
                     TEDFLGFVQRYGVFVTAEELAAYGAEELADAFPYGGAAGGGK"
     gene            3492..4217
                     /gene="hisA"
                     /locus_tag="PK614_06175"
     CDS             3492..4217
                     /gene="hisA"
                     /locus_tag="PK614_06175"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRB81834.1"
                     /translation="MRVIPAIDLKDGRCVRLRQGDMAAETVYSEDVPSVAGRWQQQGA
                     DLIHVVDLNGAVDGEPKNLPQIEAVMKTVSVKVQVGGGIRTIETVRRYLHAGVARVVL
                     GTAALMDRTFLAQACQEFPRRILLGLDARDGKVAVKGWTAVSETKAIDLLKELAGLEL
                     GAVIYTDIARDGMLNGPNLTALREVVDCSSFPVIASGGITRVEDLQAVHALGPRVEGA
                     IVGKALYDGKLDYAAAVAAIGKR"
     gene            4285..5055
                     /gene="hisF"
                     /locus_tag="PK614_06180"
     CDS             4285..5055
                     /gene="hisF"
                     /locus_tag="PK614_06180"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRB81835.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVAAIYDREGA
                     DELCFLDITASHENRKTIIDVVEQTAARVFMPLTVGGGVRTLDDIRTLLNAGADKVSI
                     NTTAVQQPEFVREAAQRFGTQCIVVAIDAKQSGQAGRWEVFTHGGRKPTGLDAVEWAQ
                     RMEGYGAGEILLTSMDQDGRQNGYDLALTAAVSERLSIPVIASGGVGTLEHLYDGLVK
                     GKADAVLAASIFHYRTHTIQQAKAYLRDRGVPVRLGPA"
     gene            5071..>5652
                     /gene="hisIE"
                     /locus_tag="PK614_06185"
     CDS             5071..>5652
                     /gene="hisIE"
                     /locus_tag="PK614_06185"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRB81836.1"
                     /translation="MSKESRTMTFDGQGLIPAVIQDWLDGTVLMVGYMNQEAIAKTLQ
                     TRSVHFWSRSRRKLWEKGETSGHFLKVKDLFVDCDRDTILVKAEPVGPTCHTGERACF
                     FARMTEEGQAGEEKTQDAGGGILDRVYQTILTRKASPQPDSYVSKLLQGGADRILKKV
                     AEEAGEVIIAAKNQKRERMEEEAGNRPKRERHRR"
BASE COUNT         1178 a         1461 c         1836 g         1167 t
ORIGIN      
        1 attgtgaatc gagccagcct caaattgaaa catgaacctc tcacggtgtt gatcgagcgg
       61 ttgcgcgccg cggtggcggt ggaagatagc aacggggcag catcgcgcgc ctaagtgtaa
      121 gtatatgagt tcgtggcggg tgtcgaagcg gcgagacaga tgatatgacc attctggcaa
      181 gtaccgatcg ggcgtatgac aaggcgttgc gaaaaatcgt gacccgcgcg cgcactcaag
      241 gcgcaggtgt ggagaagagc gtgcgcacga ttctgaaggc cgtggagcgg ggcggagatc
      301 gtgcagtcct ccggtacacg aagaagttcg atcggctgtc cttgaagctc gatcacatgc
      361 gtgtcacccc cgaagagatc aaggaggcct actaccacat tcgcaaggat gagggggact
      421 ctttgcgcta tgccgcggaa cgtgtacggc aattccatga gcgtcagcgc accaaaacct
      481 ggatgtatca ggaacaaaac gccacactcg gtcaaatggt cacgcccctg gacgccgtcg
      541 gcgtgtatgt gccgggcggc aaggctgtgt atccctcgtc ggtgttgatg tgcgccattc
      601 ccgcgaaagt ggcgggtgtt cggcgtatcg ttatgtgcac cccgaccccg aaaggggaga
      661 tcaatcccta tctgttggtg gccgcggata ttgccggtgt cgatgaaatc taccgtgtgg
      721 gcggtgtgca ggcagtcggc gcgatggcgt tcgggacgaa gaccatcgcc aaggtcgata
      781 aaattgtggg tcccggaaat attttcgtcg ccacggccaa gcgactactt tatggcaccg
      841 tcggtatcga tatggtagcc ggtcccagtg agttgctggt ggtggccgat gatgacgcca
      901 agccggcgca tgtagcggca gatctgctct gtgaggcgga gcatgacgaa gatgcgcagg
      961 tctacctcgt gacgacctcc gcgtcgttgg ccaaagaagt ggtgcgcctg attcaggtgc
     1021 agttgaaggg cttgcaacga gagaaaattg cggcgaagtc gattgcccgg cattttgcgg
     1081 cattcgtcgt tccgaccatg gatgaagcca ttgaggtggc gaatgcgatc gcgcccgagc
     1141 atctcacgct ttcggttgat cggccgttcg attatttgga gcaggtgcgg cacgccggag
     1201 ccctgttcct gggccgctac acgccgcccg cggtcgcaga ttacgtcgca ggtccgaacc
     1261 atgtgttgcc gaccggggcg acggcccgct tcttttcacc gttgtcggtc aacgattacg
     1321 tgaaggtgag caacatcgtg cactacacca aggaagagtt gacgaaagcc aaggatcata
     1381 tcgtccgact ggcgcatatc gagggcttcg atgcgcacgc caaatcagcc caaagcaggt
     1441 tcgcatgaaa aagaacggtg ctcccaggca ggcctcgctt caccgtgcta cgaaggaaac
     1501 ggacatccgc gtcgaatgga ctctggacgg caccgggcag gggactgtcg acacctccat
     1561 ccggttcttc gaccacatgc tggatttgct tgccaagcat ggtttctttg acctgacggt
     1621 ccaagccaag ggcgatattg aaatcgacga acaccacacg gtggaagatg tcgggattgt
     1681 catggggaag gtcctgcacc aggcgttggg tgaaaaggcg gggatcaagc gattcggatt
     1741 cgcctcggtc ccgctggatg aaaccctcgc ccaggtgacg gtggatctca gcgggcgccc
     1801 ttatctggtg tacaacgtca atttgccgga tcgaaagatc aagacattcg acctgggcct
     1861 cttcgaggat tttttccagg cttttgtgac ccacggtggg ctgaatctgc atgtgaatct
     1921 ccaatacgga cgcaatccgc accacattat ggaagctatc ttcaaggcgt tggcccgagc
     1981 gttggatcaa gccaccacgt tggaagagcg tttgtctggg gccgtcctct ccacgaaggg
     2041 aagcctctag gtaacagcgg tggtgtgaga ggggcagtgt ctgccggcct attcgtcgtc
     2101 caccgagtcc ccacgggccg tctccgaacg cgatatatga ttgccatcat cgattacggc
     2161 atgggtaatt tgcggagtgt ctccaaggcc tttgaggcga tgggacatca ggcggtggtc
     2221 acgcgcgagg ctcgtgtcat tgctgatgcc agtcacgtgg tgttacccgg tgtgggggcc
     2281 ttcggggatt gcatggcgaa tctggaacgg tatgaattaa tcgcgccgat tcacgccgcc
     2341 gttcaatccg gaaaaccttt tctaggtatc tgcctggggc tgcaactgct gttcacggag
     2401 agtgaagagt tcggggtgca tgcaggtctc ggcatcattc cgggtcgggt gaaaaaattt
     2461 gtgctggaac ctccgctgaa ggtgccgcat atggggtgga acgagattca aatcgtgcaa
     2521 tccgcccctc cctttgcagg catcgcgacc ggcagctatt gttattttgt gcattcctac
     2581 tacgttgagc cggttgagtc ttcggtgatc gccaccgtga ccgagtacgg gcggtccttt
     2641 gcgtccgccg tgtggaaaga caatgtcgtc gcctgccagt ttcatcctga aaaaagccaa
     2701 gctgtcggac ttcaagtgat caagaacttc ggggcctgga cgtgagagac tggtctgtac
     2761 gggctctatt gattccgatg acgttgctgc ttctggcggg ctgcagcggg acgaaggtca
     2821 cgtccaaagc ttcatcgcag gtgggaaagt atccggtccg tacaatcgcc ctcattccgt
     2881 ttgaaactat ggcgactccc caagcgatcg aggcatccgg gcccgcgttt tccgtcccgt
     2941 ccggcgcaag acggtccgac atggctgtcg gggtccctcc gacgaccgat caagccgtgc
     3001 gccagactca tcgcgtaccg gcctccgcag gtgagaaagt caccgatctg atgtgggcaa
     3061 aactcaaggc tcgatccggg ttacaactac tttcccctaa cgaatcggca agggttgcgc
     3121 gggaagtttc cggggggacc gcgacggaaa cgatgccggc ccacaagatt gctcaacgac
     3181 tcggagccga tgcggcacnn nnnnnnnngt ctatcaggaa cgagtcggca gtcggcttgg
     3241 cgcggaccca ccggccgccg tgggattcga ggtgaagctg gtagcgcccg acggcatagt
     3301 cctgtgggag ggcaactact atgaaaagca gcggccgatg acggaagact ttttaggctt
     3361 tgtccagcgc tatggcgtgt ttgtcactgc cgaggagttg gccgcctatg gagcggagga
     3421 attggcagat gcgtttccat atggcggcgc agcaggcggg gggaaatgaa cgtgttgcga
     3481 caggtggtgt catgcgtgta attccggcga ttgatctcaa agacggccgc tgtgtccgat
     3541 tgcggcaggg cgacatggcc gctgaaacgg tgtactcaga ggacgtgcct tcggtggccg
     3601 gccgctggca gcagcaaggc gccgatctta ttcatgtcgt cgatctgaac ggagccgtcg
     3661 atggggagcc gaagaatctt ccccagatcg aagcggtcat gaagacagtc agcgtgaagg
     3721 tgcaggtggg aggtggaatc cggaccattg agaccgtgcg gcgatatctc catgcgggtg
     3781 tggctcgcgt ggtgctcggc acggctgccc ttatggatcg aacgtttctg gcgcaggcct
     3841 gtcaggagtt ccctcggcgt attttgttgg gactcgacgc gcgagacggg aaggtcgcgg
     3901 tgaagggctg gacggcggtg tccgaaacga aggccatcga tctcctcaaa gaacttgccg
     3961 gccttgaact gggggccgtc atttacaccg atatcgcccg ggatggaatg ctcaatgggc
     4021 cgaacctgac ggcgttgcga gaagtcgttg attgttcgtc gtttcccgtc atcgcgtccg
     4081 gtggcatcac gcgtgtggaa gatctgcagg cggtgcatgc gctcggtcct cgcgtggagg
     4141 gggccatcgt cggcaaggcc ctctacgacg ggaaattgga ttatgcggca gcggttgcag
     4201 ccatcggaaa acgttgagga tcttgcatgc ggctggacga aaataaaacc ggctcgcttc
     4261 acgagtcacg cttgacgaac ctctatgctg actaagcgca tcattccctg tttggatgtg
     4321 aaggatggtc gagtcgtcaa aggcgtgagc ttcgtgaatt tgcgggatgc cggtgatccg
     4381 gtcgaggtgg ctgccatcta tgatcgcgaa ggcgcggacg aattgtgttt cctggatatc
     4441 accgcctcac acgaaaaccg caagaccatc atcgatgtag tggagcaaac cgccgctcgt
     4501 gtgtttatgc cgctcacggt cggaggcggg gtgcgtacgc tggatgacat tcggactttg
     4561 ctcaatgccg gggccgacaa agtcagcatc aacaccacgg cggtgcagca gccggaattt
     4621 gttcgcgagg ccgcccagcg attcggtacg cagtgcatcg tggtggcgat cgacgccaaa
     4681 cagtccggtc aggcgggtcg ctgggaagtg ttcacccacg gcggacgaaa accaaccggc
     4741 ctggacgccg tcgagtgggc gcaacggatg gaagggtatg gggccggaga gatcctcttg
     4801 accagcatgg atcaggatgg tcgccagaac gggtatgatc tggcgttgac tgcggcggtt
     4861 tcggaacgtc tctcgattcc agtgattgcc tcgggcgggg tggggaccct ggagcacctc
     4921 tacgacgggt tggtgaaggg gaaagcggac gccgtcctgg ccgcctcgat ctttcactat
     4981 cgaactcata ccattcagca agccaaggca tatctgcgag accgcggggt tccggtgcga
     5041 ctgggaccag cctagatgga gcagaaggtg atgtcgaaag agagtcggac catgacgttt
     5101 gatggacaag ggttgattcc cgccgtgatt caagattggc tggatggcac cgtgttgatg
     5161 gtcgggtaca tgaatcagga ggccattgcc aagacgcttc agacgagatc cgttcacttt
     5221 tggagccggt ctcgccggaa gttgtgggag aagggcgaga cgtccgggca tttcctcaag
     5281 gtgaaggatc tctttgtcga ttgtgatcgt gacacgattc tcgtgaaggc cgaacctgtc
     5341 gggccgacct gccataccgg tgagcgtgcc tgctttttcg cccggatgac ggaagagggg
     5401 caggccggtg aggagaaaac ccaggatgcc ggtgggggga tcttggatcg ggtgtatcag
     5461 accattctga cgcgtaaggc cagtccgcag cccgactcct atgtgtcgaa attattgcag
     5521 ggcggagccg atcgcattct caagaaggtt gctgaagagg cgggagaagt gattattgcc
     5581 gcaaagaatc aaaagcggga aaggatggaa gaagaggccg ggaaccggcc aaaaagagaa
     5641 cgacatcgca gg
//
LOCUS       DAORQN010000090         5497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_608_length_5497_cov_22.688534, whole genome shotgun sequence.
ACCESSION   DAORQN010000090 DAORQN010000000
VERSION     DAORQN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_5497_cov_22.688534"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PK614_06190"
     CDS             complement(<1..271)
                     /locus_tag="PK614_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81837.1"
                     /translation="MSLGLLVGTVLAMLAVMAGVLMGLTYLLARRGRSAPPLPFYAGG
                     VALVLVTAALVGRSELVSHSPHFVRSGLDVAAWLGASFFLFTILDA"
     gene            complement(320..2503)
                     /locus_tag="PK614_06195"
     CDS             complement(320..2503)
                     /locus_tag="PK614_06195"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRB81838.1"
                     /translation="MPHETVTELSQLIDRVKSYNVEADLDLVRRAYDFSAKAHEGQMR
                     RSGEPYFRHPLAVAGLLTHLKTDVTAIVAGLLHDTLEDTLATPLELEQRFGKDVVHLV
                     DGVTKIGKITFRNYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEYLSEGKR
                     QQIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYESLRVRVAKRDEDRQQYI
                     IEVIDLVKKAMAEAGLQGEVSGRPKHLYGIYQKMEKQSISFEEVYDLAALRIITDIKM
                     NCYALLGVIHSLWRPLPGRFKDYIAIPKSNMYQSLHTTVVGPQGEHVEFQIRTEEMHR
                     VSEQGIAAHWKYKEHGRIDEKDGKVFSWLRQFVEWNQDLPDNRQFMDSVKLDLFHDVV
                     YVFTPQGMVKELPKGSTPVDFAYSVHTEIGDHCVGAKVNGKIVPLKHMMESGDMVEIL
                     TAANQTPHRDWLKFVRTSRAKTKIKHWIKAEEQSRSIEIGKRLLEAEFRRHGLAPAQM
                     MRSEQLLAVAKQVGYETPEELAAAVGFGHVPTSQVMSKINPPVATEGQTVAPEVPPPP
                     KPVTGRTDDTGVQVKGARDLLMQLSRCCNPVPGDRILGYITRGRGLTIHTVDCPNLEA
                     LDYDKNRLVEVEWDHSTPSTHSVKVSVIAVDKTGVLAHVSTAISECQANISRAEITTR
                     EDRKAMLDFIIEVLDTAHLARVLKAIERVDGIITVRRVRSWQERS"
     gene            complement(2506..4212)
                     /gene="recJ"
                     /locus_tag="PK614_06200"
     CDS             complement(2506..4212)
                     /gene="recJ"
                     /locus_tag="PK614_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRB81839.1"
                     /translation="MQEKLWVFRPSDIALQNNLSRQLSISPVTASVLLARGVMTPDEA
                     TRWMSGVQGGLHDPFLLPDMEPAVERLHQALARQERVCFYGDYDVDGVSATSLYLSFY
                     QGLGGNACGYIPHRVREGYGLNEGAVRHLAEEGISLLVTSDCGTTSHHEIAVARELGM
                     DVIVTDHHQTDATMPAALAVLNPHRRDAIYPFKGLCSAGLAYKVVSAYQQRYGSGDVE
                     PDSCLDLVALATVADIVPLQDENRILVRDGLIQITRGARAGIRALKQVAGIAKACTSE
                     TIGFRLGPRLNAAGRLDHAMLCVRLLTTESDQEAMQIAEQLEQLNRQRQQIEEGITGE
                     AATYLQQDEPAAAIVLGSRDWHLGVVGIVAARLVDRFHRPSIVMAIDGQGIGKGSART
                     VEGFDLYQGLSECKDLLEAYGGHPSAAGLTLKESRLEEFRRRFCEVVAQWAGSARTVP
                     TLHVDAEVKLTDVNFDLIQELESLHPFGAGNPEPTLAVRGLDVVDARVVGEKHLKLRV
                     RQGRSIIFDSIGFRMGSFEELGLRAGRPVDLAFSPERNHWNGYDRVQLRIKALRMSGE
                     VS"
     gene            complement(4421..4618)
                     /locus_tag="PK614_06205"
     CDS             complement(4421..4618)
                     /locus_tag="PK614_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HRB81840.1"
                     /translation="MEKQERRPESRKDSHQKEEVKPNPKVVESGKKMKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            complement(4781..>5497)
                     /locus_tag="PK614_06210"
     CDS             complement(4781..>5497)
                     /locus_tag="PK614_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRB81841.1"
                     /translation="AHLGKEVRDITDPLDAVGNTTKAVTKGYAIGSAALAAVVLFAEF
                     AREVAKGSQASTFDLSNPLVLVGLFLGGMLPFIFGALCMKAVGQAAGLVVEEVRRQFR
                     TIKGIMEGTGKPEYGTCVDIVTQAAIQKMMIPGLIPVVAPILVGVILGPQALGGVLVG
                     SIVTGLFVAISMTSGGGAWDNAKKYIEEQGLKGTDTHKAAVTGDTVGDPYKDTAGPAI
                     NPMIKVINIVALLIVSLIVK"
BASE COUNT         1155 a         1781 c         1453 g         1108 t
ORIGIN      
        1 gggcatcaag aatcgtaaag agaaaaaaag atgcgcccag ccaggccgcg acatcgagtc
       61 ccgaccggac aaagtgcgga ctgtgactca cgagttcgct gcggccgacc aacgcggcgg
      121 tgacgagcac cagtgccacg ccgccggcat agaacgggag cggcggcgcc gacctgccgc
      181 gtcgagccaa cagataggtc aggcccatca acaccccggc catgacggca agcatcgcca
      241 acaccgtgcc gaccagtaga ccgaggctca tgcggatctc acctgatgct gggaagagaa
      301 cacacgaaca cctctcgggc taggaacgct cttgccagga gcgaacacgt cgcacggtga
      361 tgatgccatc caccctttca atcgccttga gcacccgagc caaatgtgcg gtgtccagga
      421 cctcgatgat gaaatccagc atcgccttcc ggtcctcgcg ggtcgtaatt tcagcccgac
      481 tgatattggc ctgacattcg gagatggccg tcgagacatg cgccagcact ccggtcttgt
      541 ccacggcgat gaccgagacc ttgacggagt gtgtactggg cgtggaatgg tcccactcga
      601 cttcgaccag ccggttcttg tcatagtcca acgcttccaa attgggacaa tccacggtat
      661 gaatcgtgag tccacgtccc ctggtgatat accccaagat gcggtcgccc ggcacaggat
      721 tgcaacagcg ggagagttgc atcaacaagt cccgcgcgcc tttgacctgc acacccgtgt
      781 catcggtccg tccggtgacc ggtttcggcg gcggcggaac ctcaggggcg accgtctgtc
      841 cttccgtcgc gacgggcgga ttgattttgc tcatcacctg cgaggtcggc acatggccga
      901 agccgacggc tgcggccagt tcctccggcg tttcataccc gacctgtttg gccacggcca
      961 ggagctgttc cgaccgcatc atctgggcgg gcgccaggcc atgccggcgg aactcggctt
     1021 ccaacaaccg cttgccaatc tcgatgctac gggattgctc ttcggccttg atccaatgtt
     1081 tgatcttggt tttcgcgcgc gacgtgcgga cgaatttcaa ccaatcccgg tgaggcgttt
     1141 gattggcggc ggtcaggatc tccaccatat ctccgctttc catcatgtgt ttcagcggaa
     1201 cgatcttgcc gttgaccttc gcccccacac agtggtcgcc gatttcggtg tggacagaat
     1261 aggcaaaatc caccggagtg gagcctttgg gcaattcctt caccatgccc tggggggtaa
     1321 agacgtagac gacatcgtga aaaagatcca gcttcaccga atccatgaac tgacgattgt
     1381 ccggcaaatc ctgattccat tccacaaact ggcgtagcca actgaaaacc tttccgtctt
     1441 tctcgtcgat ccgcccgtgc tctttgtatt tccagtgtgc ggcgatgccc tgttcagaaa
     1501 cgcgatgcat ctcctccgtc cgaatctgaa actcgacatg ttcgccttgg ggaccgacga
     1561 cggtggtgtg caacgactga tacatgttgg atttcggaat cgcgatgtag tccttgaagc
     1621 ggcccggcaa cgggcgccac agtgaatgga tgacaccgag gagcgcataa cagttcatct
     1681 tgatgtcggt aatgatgcgc agcgcggcga gatcgtacac ctcttcgaat gagatcgatt
     1741 gcttctccat cttctggtag atgccgtaca ggtgtttggg gcggccggag acttcaccct
     1801 gcaaaccggc ctcggccatg gccttcttca ccaggtcgat gacttcaatg atgtactgct
     1861 gccggtcttc gtcccgcttc gccacccgaa cgcgcaacga ctcgtagaca tccggcttga
     1921 gatgtttcag acagaggtct tccagctcgt tcttaatcca accgatcccc agccgattgg
     1981 ccaacggagc ataaatttcc agcgtctcct gggcgatttg ttgccgcttc ccctcgctca
     2041 gatattccag ggtccgcatg ttgtggagcc gatccgccag cttgatgagg acgacgcgga
     2101 tatcgtccgc catcgacaag accatcttgc ggaaattttc cgcctgcttt tcctcgtagt
     2161 tccgaaaggt aatcttgcca atcttggtca cgccgtcgac cagatgcacc acgtccttcc
     2221 cgaatcgctg ttccaactca agcggcgtag ccagcgtatc ttccagcgtg tcgtgcagca
     2281 ggcctgcgac gatggccgtg acatccgtct ttaaatgggt cagcaggccc gcgaccgcca
     2341 gaggatgccg aaaatagggc tcaccggatc ggcgcatctg tccttcgtgc gccttggccg
     2401 aaaaatcata agcccggcgc acaagatcga ggtccgcctc gacgttgtag ctcttcaccc
     2461 gatcgatcag ctgactcagc tctgtcaccg tctcgtgcgg catagttacg acacctcacc
     2521 gctcatgcga agggccttga tacgcaactg cacccgatcg tagccgttcc aatgattgcg
     2581 ctcggggctg aaggccaagt ccaccggtcg accggccctg agccccagct cttcaaacga
     2641 gcccatgcga aagccgatgc tatcgaagat aattgaccgc ccttgccgaa cacgcaactt
     2701 cagatgcttc tcgccgacga ctcgcgcgtc caccacatcc aggccgcgaa cagccaaggt
     2761 gggttcggga ttgccggccc cgaagggatg cagggattcc agttcctgga tcaggtcgaa
     2821 attgacatcc gtcagcttca cctcggcatc gacgtgcagg gtcggcaccg tgcgagcact
     2881 cccggcccac tgagccacca cctcacagaa ccgccggcgg aattcctcca accgcgattc
     2941 cttgagcgtc agtccagcgg cactcgggtg accgccatag gcctccagca gatccttaca
     3001 ctcggacaat ccctgataca gatcgaaccc ctccaccgtc ctggccgagc ccttccctat
     3061 gccctgccca tcgatcgcca tcacgatgct cggacgatgg aaccggtcca ccaagcgagc
     3121 ggcaacaatc cccacaacac ccagatgcca gtcacgcgag cctaagacga tcgccgcggc
     3181 cggctcatcc tgctgcagat aggtcgccgc ctcgccggta atgccttcct cgatctgttg
     3241 ccgctgccgg ttcaactgct ccaactgttc ggcaatctgc atggcctcct gatcggactc
     3301 cgtcgtcaac agccgtacac agagcatggc atgatccagc cgtcctgcgg cattcaagcg
     3361 ggggccgaga cggaaaccga tcgtctcgct cgtacaggcc ttcgcgatac cggctacctg
     3421 cttgagcgcg cgaattccag cgcgggctcc gcgcgtgatc tggatcaggc catcccgcac
     3481 cagaatccga ttctcgtcct gaagcggcac gatgtcggcc accgttgcca acgcgaccag
     3541 atccaagcag gagtctggct ccacgtcgcc ggatccatac cgttgctggt aagccgacac
     3601 caccttatac gccaaccccg ccgagcagag ccctttaaac ggatagatcg catcgcgccg
     3661 gtgcggattg agcacggcaa gggcggcagg catcgtggca tccgtttgat gatgatccgt
     3721 gacgatcaca tccatgccga gttccctggc gaccgcaatc tcatgatgcg aggtcgtccc
     3781 gcagtcggag gtgacaagca gcgaaatccc ctcttccgct aagtgacgaa ccgccccctc
     3841 gttcaacccg tagccttcgc gaaccctatg cggaatgtag ccacaggcat tcccgccgag
     3901 accttgatag aacgacagat agagactcgt cgccgagacc ccatcgacat cgtaatcgcc
     3961 gtaaaaacag acccgctcct ggcgagcgag ggcctgatgt aaccgctcga cagccggttc
     4021 catatcaggg agcaaaaatg gatcgtgcag cccgccctgc acgccggaca tccaacgggt
     4081 cgcttcatcg ggagtcatga caccgcgcgc aagcagcacc gacgccgtca ccggcgagat
     4141 cgagagctgc cgcgacaaat tgttctgcaa ggcgatatca gacggtcgaa acacccacag
     4201 tttttcctgc atgaatcctc tgtctttgcc tgaaatattc agaagttact gagaagtaag
     4261 gggaatggta tcgactcacc atttttttgt caaacgggtt ctcgcgccgg attaggacga
     4321 ggcgcagcgt cgggtcacga tcagccctct gatgaacgga cgaagcgtcg tggcacaaaa
     4381 aggagggaag gaccgggagc ggataacaaa agggagggga ctactcccct cccttttgca
     4441 catagttctt cacgaactct tcggcatttt tttcgagcac gtcatcgatt tcatcgacca
     4501 acttgtcgat gtcctctttc atttttttcc cggactcgac gactttggga ttcggcttga
     4561 cctcttcctt ctgatgagag tctttcctgg attccggtct cctctcctgt ttctccatcg
     4621 gagcacctcc agtcaatcag aacctgttcg aaccacccac tccttgtcac cttactctag
     4681 ggtcgtcgaa cgcgtcaagc caaggaacac attgtgtacg gagtcgctcg cgtaacgccc
     4741 ggaacgccca cgaacgacca accccacctc acacccgcag ctatttcaca atgagcgaga
     4801 cgatcagcag cgccacgatg ttgatgactt tgatcatcgg attgatcgcc ggcccggccg
     4861 tatccttgta cgggtcgccg accgtgtccc cagtcaccgc cgccttatgc gtatcggttc
     4921 ccttcaggcc ctgctcttca atgtacttct tggcattgtc ccaggcgccg ccgccgctcg
     4981 tcatggagat ggccacaaag agacccgtca cgatgctccc caccagcact ccgccaagcg
     5041 cctgtgggcc aaggatgacg cccaccagaa tcggggccac gacgggaatc aaccccggaa
     5101 tcatcatctt ctgaatcgcc gcctgcgtca cgatatccac gcaggtgccg tactccggct
     5161 ttcccgtacc ttccataatg cctttgatcg tccggaactg gcgccgaacc tcttccacaa
     5221 ccagcccggc cgcctggccg accgccttca tacacaacgc gccgaaaatg aacggcaaca
     5281 ttcccccgag aaagagcccg accagtacca agggattgga gagatcgaag gtgctggctt
     5341 ggctaccctt ggccacttca cgggcgaact ccgcaaacaa gaccaccgcc gcgagtgcgg
     5401 cagagccgat cgcatagccc ttggtgaccg cctttgtcgt attcccaacc gcgtcgagcg
     5461 gatccgtgat gtcgcgcact tctttcccca gatgggc
//
LOCUS       DAORQN010000091         5463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_614_length_5463_cov_6.389608, whole genome shotgun sequence.
ACCESSION   DAORQN010000091 DAORQN010000000
VERSION     DAORQN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_5463_cov_6.389608"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PK614_06215"
     CDS             complement(<1..1318)
                     /locus_tag="PK614_06215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRB81842.1"
                     /translation="MTVNGLAQIGLFFFVLVALVKPLGWYMARVYTGQACGMDRVMGP
                     FERLIYRVCGVRATEEMDWKTYAVAMLLFNVTGLVALYALQRLQGYLPLNPAGFGAVA
                     PDLAFNTASSFITNTNWQAYAGESTLSYLTQMLGLTVQNFVSAATGMAILVALIRGLT
                     AQTAATIGNFWVDLTRSTLYILLPLSAVLALLLVSQGTVQTFGSSHHTTLLQSVTYEK
                     PIVDAAGQPVLDEKGAAKTESTAGTEQALAVGPVASQVAIKHLGTNGGGFFNANAAHP
                     YENPTPLTDFMLILAETVIAAALPYTFGTMVGDTRQGWAILAAMLSVLALFVLGAYWA
                     ESAGNPRLTMLGVEQTAGSAQSGGNMEGKEVRFGVARSALFATATTATSTGAVNAMHD
                     SFTPLGGLVPLFMMQFGEVIVGGVGSGLYGMLIFAIIAVFIAGLMVG"
     gene            complement(1318..1407)
                     /gene="kdpF"
                     /locus_tag="PK614_06220"
     CDS             complement(1318..1407)
                     /gene="kdpF"
                     /locus_tag="PK614_06220"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HRB81843.1"
                     /translation="MNAMYALGAVLSLGLLVYLMIALLKAEWF"
     gene            complement(2066..2587)
                     /gene="nuoB"
                     /locus_tag="PK614_06225"
     CDS             complement(2066..2587)
                     /gene="nuoB"
                     /locus_tag="PK614_06225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRB81844.1"
                     /translation="MFRILKKSLQTGVVTGHHPAGAAPSEPVSQDAIEKAKPFRRSLT
                     IREVDTGSCNACEMEMNALANPVYDVERFGVHIAASPRHADALVVTGPVTVNMERALK
                     DVYKATADPKIVIALGDCAVNCGLFKGSYAVTGPVDRHIPVDVRISGCPPRPAEILAV
                     LGALRKNKADSDQ"
     gene            complement(2640..4232)
                     /locus_tag="PK614_06230"
     CDS             complement(2640..4232)
                     /locus_tag="PK614_06230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRB81845.1"
                     /translation="MIDASPAVEQVQAAFTQAITEVCAVHGVPMLRVKKQDLPTVAHF
                     LHTNPGVRGSLSLLWAVDHRPREARYELCYLFTLAERKDWLLLATDLHGDEREFPSIT
                     PHLHAAKWYEREIRDLFGLIPVGHPDLRRLVRHEHWPKGSHPLKKDFLWDRVLGRVQG
                     EYAFRKIHGEGVFEVPVGPIHAGIIEPGHFRFSVAGEPIMQLEVRHFWKHRGVEKLFE
                     QQRLTEAVPLSERVSGDTSVGHSLAYCQAVETLHGVEVSPRARYLRTLFLELERLHNH
                     IGDVGAICNDTAYALAHAHCGRMKEQLMQLNDRLTGSRFLRGVNCVGGVALDLSGLQL
                     AQVEVELNTLEQDFSAIEKIVFANASLTERLENTGILTEGIAWDHAVIGVVGRASGID
                     RDLRRDRPFAAYDVLQPKVALYRYGDVRARMRVRLDEIHESIRLIREVRRGLPLGRLV
                     TRPMHEAQPGVWALSAVEGWRGEILYVVMAGDAGRIHRCKVRDPSFVNWPAIQWAALG
                     NIVPDFPLINKSFNLSYAGNDL"
     gene            complement(4237..>5463)
                     /locus_tag="PK614_06235"
     CDS             complement(4237..>5463)
                     /locus_tag="PK614_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRB81846.1"
                     /translation="RLGHFFFLFHMFLFAMVAATVANSLGVQWVALEGTTLATTFLIA
                     FFRRRESLEAGWKYLILCSVGIALALFGVVLTYYSSVRVLGDASSALNMTALIGVANQ
                     LDPNVLKLAFIFMLVGYGTKVGLVPMHTWLPDAYSEAPAPIAAMLAGVLETVAVYTVL
                     RSKALVDQALPPEFTGNLLLTFGLLSFIVASLFILIQHNYKRLFAYSSIEHMGLAMIG
                     FGVGGMIGTFGGLFHLLNHAVAKALAFFVAGNIHRRFDTLEIDGVRGLARAQPITAVA
                     ILVAGCALVGLPPFSPFISELLVVSAVAAQDFSSDTMHVGRFVTMTISDEMRSLGIVA
                     LFLFFAVVLFGGFMFRVGAMVWGTAPAGIAQGESWTVGHVPLMIMIVALLGLGFVLPD
                     PIQTLLTRAVNVIVVR"
BASE COUNT         1277 a         1699 c         1524 g          963 t
ORIGIN      
        1 ggcctaccat caggccggca atgaacaccg cgatgatcgc gaagatcaac atcccgtaga
       61 ggcccgagcc gaccccgccc acaatcacct cgccgaattg catcatgaac agcggcacca
      121 acccgccgag tggcgtgaat gaatcgtgca tggcattcac cgctccggtg gatgtggccg
      181 tcgtcgcggt ggcaaagagc gccgaacggg cgaccccgaa acgaacttcc ttcccttcca
      241 tgttgccgcc ggattgcgca ctcccggcgg tctgctcgac gcccaacata gtcaggcgtg
      301 gatttcctgc ggactccgcc cagtaggccc ccagaacgaa gagggccagc acgctcagca
      361 tcgcagcgag gatcgcccag ccctgcctgg tgtcgccgac cattgttccg aacgtgtagg
      421 gcagagcagc ggcaatcacg gtttcagcca ggatcagcat gaaatcggtc agcggcgtcg
      481 ggttttcata gggatgagcg gcattggcat taaaaaatcc gccaccgttt gttccgaggt
      541 gcttgatggc tacttgtgac gcaaccggac cgacggccaa ggcttgctca gtccctgctg
      601 ttgattcggt cttggcagcg cccttctcgt ccaacaccgg ctgccctgcg gcatcgacta
      661 tcggcttctc ataggtcacg gactgaagaa gcgtggtgtg atgagaagac ccgaaggtct
      721 ggaccgtccc ttgcgagacg agaagcagcg cgagaacggc tgacagcggg agtaagatgt
      781 acagtgtgct gcgggtcaga tcgacccaga aattgccgat ggtggctgcc gtttgagcgg
      841 ttaacccgcg aatgagggcg accaggatgg ccatgcccgt ggcggcagag acgaagtttt
      901 ggaccgtcag gccgagcatt tgcgtgagat aactgagcgt cgactcaccg gcataggcct
      961 gccagttcgt attcgtgatg aagctcgagg cggtgttgaa ggcgaggtcg ggagccacgg
     1021 ccccgaaacc agccgggttc agcggcagat atccctgcag tcgctgcaac gcatagagcg
     1081 cgaccaaccc cgtgacattg aacaacaaca tggccacggc ataggtcttc caatccatct
     1141 cctcggttgc acgcacgccg cagacacgat aaatcaggcg ttcgaagggg cccatcaccc
     1201 ggtccattcc gcaggcttgg cccgtataga cgcgtgccat gtaccaaccc agcggcttga
     1261 ccaacgctac caggacgaaa aagaagagtc cgatttgagc caacccgttg acagtcatca
     1321 gaaccactcc gccttcagca gcgcgatcat gaggtacacc aacaacccca gtgacaacac
     1381 tgcaccgagc gcgtacatcg cattcatgac cacaccccct agagcaggat tctgaaaaag
     1441 tccgccagct tcgttgtcgc atcattcaga tcctcaacgt accccaaggg tacgcctcgg
     1501 accttcattc gctggggcct tgctggacag cctttttgag catcctgcgg tgatgctgtt
     1561 ctattagact gcctctccgg agcaatgaat ttcacgtgtg acaacaaagt ttttccgtag
     1621 gctgctagag acggtccaac gccgagatga gccagccgga taacagaaaa aataccgcgg
     1681 cgatcgtcag gtagatgagg tccatgtaca tgctccttac cgggacgtaa aacttagtgt
     1741 ggcgcgcttc gcccgaaaca cctatccaag cggtcgccaa ccggccacat ctcttcacgc
     1801 ctcttctctt ccactccata ctcaaggtaa cgcgcagaaa gtaaaaacgg tgtaaaaagg
     1861 ctaagaaaat ggatttcctg aaaagatctt cccggacagc tgttcacgca tttggggcgg
     1921 atcgcttcag atgacggaag agtcgaaaca catcaactcg cgagggcggc gctgaagaag
     1981 ggatgtagac gaaagtgtga ccgtctattg agcaggtcga aaagatttgt tggatgaacg
     2041 ggagaggcgt gactgtcttc gggcgctact gatctgaatc cgccttattt ttacgtaacg
     2101 ctcctagcac cgccaggatc tccgccgggc gtggggggca accggagata cgcacatcga
     2161 cggggatatg ccgatccacc ggccccgtca ccgcataact ccctttgaac aggccgcaat
     2221 tcacggcaca gtctcccaac gcgatgacga tcttaggatc agctgtcgcc ttgtagacat
     2281 ctttcaacgc acgctccata ttgacggtca ccggtcccgt cacgaccagc gcatcggcat
     2341 gacgcggcga cgcagcaata tggaccccga accgctccac gtcatagacc ggattggcca
     2401 gcgcattcat ttccatctcg caggcgttgc aggaacccgt atcgacttcg cggatcgtca
     2461 gcgagcgacg gaacggcttg gccttttcga tggcgtcctg gctgaccggc tccgaaggtg
     2521 cggctccggc aggatggtgc cccgtgacga caccggtctg aagacttttt ttgaggattc
     2581 gaaacatggc tcctccctcg ggaacgtaat cagcgagtca gtaatttaat cgaccattgc
     2641 tagagatcgt tcccagcgta agacaaattg aaactcttat tgatcaacgg aaagtccggc
     2701 acgatattgc cgagcgccgc ccattggatc gccggccaat tcacgaagga cggatcgcgc
     2761 actttgcagc ggtgaatccg acctgcgtcc ccggccatga cgacatacag aatctcgcct
     2821 cgccatcctt cgaccgccga cagcgcccat acacccggct gtgcttcgtg catcggccgg
     2881 gtcacgagcc ggcccagtgg gagaccgcgg cggacctcgc gaatgagacg gatcgactca
     2941 tggatctcat ccagacgcac ccgcatcctg gcgcgcacat caccatagcg gtagagggcg
     3001 accttcggct gcagcacgtc gtacgcagca aagggccggt cgcgacgcag atctcgatcg
     3061 ataccggagg cccggccgac cacaccaatc accgcatgat cccaggcgat cccttccgta
     3121 aggatgccgg tgttctccag ccgttccgtg agcgacgcgt tcgcaaacac aatcttttca
     3181 atcgccgaaa aatcctgctc aagggtattc agttccacct ccacctgcgc gagctgcagg
     3241 ccggacagat caagcgccac tccgccgacg caattgacgc cacgcagaaa acgcgacccg
     3301 gtcaggcgat cgttgagctg catgagctgc tctttcatcc ggccgcagtg ggcatgggcc
     3361 agggcatagg cggtgtcatt gcagatggct cccacatcgc cgatgtggtt gtgcaaccgc
     3421 tccaactcca gaaacagcgt gcgcagatat cgggcacggg gcgagacctc cacgccgtgc
     3481 aaggtctcga cggcctggca ataggccagg ctatggccga cgctcgtatc gccggagacc
     3541 cgttcggaca acggtaccgc ttcggtcaac cgctgctgct caaacaattt ctcaacgccg
     3601 cgatgtttcc agaaatggcg cacttcgagc tgcatgatgg gctcgccggc caccgaaaat
     3661 cggaaatggc ccggttcaat gatgcccgca tggatgggac cgacgggaac ctcgaaaacc
     3721 ccctcaccgt gaattttccg gaaggcatac tccccctgca cccgtcctaa cacccgatcc
     3781 cagagaaaat cttttttcag cgggtgggac cccttcggcc aatgttcatg acgaacgagt
     3841 cgacgcagat cgggatggcc gaccggaatc agtccgaaca gatcccgaat ctcccgttcg
     3901 taccatttcg ccgcatgaag gtgaggagtg atcgatggaa attcccgctc atctccatgg
     3961 agatccgtcg cgagcagcag ccagtctttg cgttccgcca acgtgaagag atagcagagc
     4021 tcgtagcgcg cttcgcgagg gcgatgatcg accgcccaga gcagcgacag cgatcctcgc
     4081 acacccgggt tcgtatgcag aaaatgggcg accgtcggca gatcctgctt cttcaccctg
     4141 agcatcggga cgccgtgaac ggcgcacacc tccgtgatgg cctgcgtaaa cgccgcctgc
     4201 acctgctcga ctgccggact tgcgtcgatc atacggtcac ctcaccacaa tcacgttgac
     4261 ggccctggtc agcagcgtct gaatcggatc aggaagaacg aatccaagcc ccaacaacgc
     4321 gacgatcatg atcatcagcg gcacatggcc gacggtccac gactctccct gggcgatacc
     4381 ggccggggca gtgccccaca ccatggcccc gacgcgaaac ataaacccac cgaacaacac
     4441 cacggcaaaa aacagaaaca gcgcgacgat gccgaggctg cgcatctcat ccgagatcgt
     4501 catcgtcaca aaccgtccga catgcatcgt atcggaggaa aagtcttgcg cggccactgc
     4561 cgacacaacc agcaactcac tgatgaacgg cgagaaggga ggtaagccga ccaacgcgca
     4621 acccgccacg agaatcgcca cggcggtaat cggctgcgca cgcgccagcc ctcgcacccc
     4681 gtcgatttcc aaggtgtcga atcgccgatg gatattgccc gcgacgaaaa aggccagcgc
     4741 cttggccacg gcgtgattga gcagatgaaa gaggccgccg aaggtgccga tcatcccgcc
     4801 gactccgaag ccgatcatcg ccagccccat atgttcgata ctcgaatagg caaacagccg
     4861 cttgtagttg tgctgaatca ggatgaatag agaggccacg atgaacgaga gcagcccgaa
     4921 ggtgagcagc agattgccgg taaactccgg gggaagcgcc tggtccacca gcgccttgct
     4981 gcgcaacacg gtatagaccg ccaccgtctc caacaccccg gccaacatcg ccgcaatcgg
     5041 cgccggcgct tcgctgtagg catcgggtaa ccaggtatgc atgggaacca ggcccacttt
     5101 cgtgccataa ccgacgagca tgaaaataaa cgccagcttg agcacattgg ggtccaattg
     5161 gttggccacg ccgatcagcg cggtcatatt gagcgccgag ctcgcatcgc caaggacccg
     5221 cacggaggag taataggtga gcaccacgcc gaagagcgcg agggcgatac ccaccgaaca
     5281 gaggatcaga tatttccaac cggcttcgag ggattcccgc ctgcggaaaa atgcgatcaa
     5341 gaaggtcgtc gcgagggtgg tcccctccaa cgccacccac tgcacgccga ggctgttcgc
     5401 taccgtcgcg gcgaccatcg caaacaggaa catatggaag agaaaaaaga agtgaccgag
     5461 acg
//
LOCUS       DAORQN010000092         5409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_620_length_5409_cov_31.508592, whole genome shotgun sequence.
ACCESSION   DAORQN010000092 DAORQN010000000
VERSION     DAORQN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_620_length_5409_cov_31.508592"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..2261)
                     /locus_tag="PK614_06240"
     CDS             complement(42..2261)
                     /locus_tag="PK614_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81847.1"
                     /translation="MHGEEWIREQIRQNRPISFPVDWPENHRSISGEFFLELLLDPCW
                     KASGGGFVIKNAVVNSEVYKRYLTCETECYLENCEFVNGFGLPYANLKRVLRFKSCKF
                     GGQFNLHSSHLADVEFLPSEDGHTTQFLQPANFMRCVVSGLILCDKCLFRCKTVNFNS
                     VKVRGNVNLSYSVFEGNVDFTGAKIAGQFNATDTRFTNQESEVAFVGSELSGGAILDS
                     ARFSGGANFRAAKFGLRLQCEGVAFLNKDKEIAFDSCKVAGTAMFENARFFSSLDLSS
                     SHIEGDLACKGAHFSNEKTSATFNSMTVLQNAFLVEAVFSGPSSFISVHIERNLICAL
                     ARFETESANLNMQSMKVGGNAIFDRALFMGNVDLGGIGIAGQLVVKDSKFPGAPNGLS
                     LNGAKVLRGLFLNGTHFNCPLDLTGGEIGAQLGCRDVCFAKEDGTISFSNVKVHGDLS
                     IDNATFAGPFSLVGAVIEKQLRCTEANFQTTAAVALSGIVVGTSAVFSNSSFQNEVVF
                     RYARVGQNLSFAGCQFHTSLDLRNSLVAGYLYVFSIPENSVPLAQTQKTKLPTIAQFS
                     GLRYSGTDIGQSELWREWLKPTCAGLNYEADSYLAVEKCFRLMGLSEEADRVYYEMKN
                     VETWLSLRRWRIDRWGKGMLLRWLVGYGVYGYRLWCWVLLPPLIIFFWCRVSQPVFNQ
                     LFVMLYAVDIFLPIDLHQDAYCRLPTWATAVIELLGWVLVPIVIAQLTGYLKRNAET"
     gene            2467..2766
                     /locus_tag="PK614_06245"
     CDS             2467..2766
                     /locus_tag="PK614_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81848.1"
                     /translation="MSPKPKNTPHRVLLSLPTDLYNTISRVSAAMEKPRAEIIRELLA
                     EQQPILEHMATMLEQAKAGKLDQAMKGWQKMTGEALKGLGVLMSPPDIKKSKPKA"
     gene            2824..3045
                     /locus_tag="PK614_06250"
     CDS             2824..3045
                     /locus_tag="PK614_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81849.1"
                     /translation="MSEQVLKRLYSVREAARYLGVSPWSVRNLQKLRTLPCVRQGRRV
                     LFDIHDLERHIENHKTEVHDGHDLSPQET"
     gene            3011..4315
                     /locus_tag="PK614_06255"
     CDS             3011..4315
                     /locus_tag="PK614_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB81850.1"
                     /translation="MMGMIYRRKKRDPTTGKLEEQGPYWMKYYIEGRAIQESTKVFEK
                     EQAKKILKVKEGEVAAGIYRGPSIDRIRFEDLAALVQQDYQINRRKTVRRITEYQKHL
                     EPYFRKARVSSMTTERIKAYIIKRQEQGAANGTINREIGFLKRMFRLGYQQTPQLVAR
                     VPHIPQLKEHNIRSGFFEHEDFLALRGALPDYAQVPATLAYYSGMRMGEVYSLQWRQI
                     NWTEGKLFLKAQDTKTNTPRVLYLTGDLYRVLLAWKTRCDKKWPACQWICHRGGIRLQ
                     SFKRSWWEACEAVGLGHRVKDERKGRLVWDGKRPHDFRRTAVRNMIRAGVPEKIAMAI
                     SGHKTRSVFDRYNIVNERDLEQAARSLTAYFEKQTVTLAVTLAELRGESSGVANRQQV
                     ETSGIFLEPASGIEPPTCGLRIRVNPLPLLYFPIFSLQTTAN"
     gene            4482..>5409
                     /locus_tag="PK614_06260"
     CDS             4482..>5409
                     /locus_tag="PK614_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB81851.1"
                     /translation="MHHIIEDGAEREHLAGCSSCRNGETVPFDCSIKLRGEIERRAAF
                     YGRTWDEELNHILAVAYEQREPDAGDADMERRLNELRRRGELPSQKRSKVEIRLASID
                     RLPDGVDVPRPTTVKELCNIYLEYKLKGRRSYANVRRTIRHTMEQTGFADVKLRDLTP
                     GVIKRWHAGMASRHVTGNNGLKTLRSAYRFCRSLELLNEDPTAGIKRFREDARERYVT
                     PSEMPRLMHALESAPLRAQTFFLTILFTASRPGEVAGMQWKDLDFVGMRWRKPTSKTG
                     RSQEVVLNPIVYRLLRQLPQVSDYVFPGQDLSK"
BASE COUNT         1467 a         1392 c         1358 g         1192 t
ORIGIN      
        1 agctggcgcg atgggctagt gtcgctccca gtattctcac tctaggtttc cgcgtttctt
       61 ttcaaataac ctgtcaactg agcaataaca atgggaacga gcacccaacc taatagttcg
      121 ataacagccg tggcccaggt aggaagcctg caataggcat cttgatgcaa atcaatagga
      181 agaaatatat cgactgcata taacatgacg aacagctggt taaaaacggg ctgagatacg
      241 cggcaccaga agaaaattat cagcgggggt agtagcaccc agcaccataa tcgatacccg
      301 tagactccat aaccaactag ccaacgcaac agcattccct tgccccatcg atcgatccgc
      361 cacctgcgca aggaaagcca tgtctcaacg ttcttcatct catagtagac gcggtcggcc
      421 tcttcgctta accccattag tctgaaacac ttctccactg ctaaataaga atccgcctca
      481 tagttgagac cagcacaggt aggtttcaac cattccctcc atagctcact ttggccaata
      541 tcggttcccg aatatctaag tccgctgaat tgggcaatag taggaagttt ggtcttttgt
      601 gtctgagcaa gaggaaccga gttttctgga attgagaaaa catatagata accggctacc
      661 aaactattgc gaagatccag agatgtgtga aattggcagc ctgcgaacga cagattttgt
      721 ccgactctcg cgtacctaaa gacaacctca ttttgaaatg acgaatttga gaatacggca
      781 cttgttccaa ctacaatccc gctcaatgct acggcagccg tagtttgaaa atttgcttca
      841 gtacatcgta gttgcttttc aatcaccgcc cccaccagcg aaaacggccc agcaaacgtt
      901 gcgttatcga tactcagatc accatgaact ttgacattag aaaaagatat tgtcccatcc
      961 tctttggcga agcagacgtc acgacagcct aactgagcac cgatctcccc accagttaaa
     1021 tccaaaggac agttaaaatg ggtgccattg agaaataagc ctctgagaac ctttgctcca
     1081 ttcagtgaaa ggccattggg tgctcctggg aacttagaat cctttacaac caactgtccg
     1141 gctattccga taccaccaag gtcgacattg cccatgaaaa gggcgcgatc aaatatcgcg
     1201 ttgcctccca ctttcataga ttgcatattc aaattggcag actcggtttc aaagcgtgct
     1261 aaggcacaaa ttaagtttct ttcgatatga acagaaataa aagaagaagg accgctgaat
     1321 accgcctcaa ccaagaatgc attctgcagt actgtcatag agttgaatgt ggcggaagtt
     1381 ttttcattag agaagtgcgc ccctttgcat gccaagtcgc cttcaatgtg agaggaagag
     1441 aggtccaatg agctgaagaa gcgagcgttc tcaaacatcg ctgtccctgc aaccttgcac
     1501 gaatcgaatg cgatttcttt atctttgttc agaaaagcca ccccttcgca ttgcagtcgc
     1561 agtccaaatt tagcagcacg aaagtttgct cctccagaaa agcgcgcaga atcaagtatg
     1621 gctcctccgg atagctcact gcctacaaag gctacctcgg attcttggtt ggtgaaccgc
     1681 gtgtccgtcg cattaaactg tcctgcgatt ttggctcctg taaaatcaac gtttccctcg
     1741 aacacgctgt aggacagatt cacattgcct cgcactttca cagaattgaa gttcactgtc
     1801 ttgcacctaa ataggcattt gtcgcaaagg atgagtcctg agacaacgca tcgcataaaa
     1861 tttgccggct gaaggaattg tgttgtgtgt ccatcctcgg aaggtaagaa ttccacatct
     1921 gctagatggg aagaatgcag gttaaattgc ccgccgaact tacagctctt aaaccgtaat
     1981 actcgtttca ggttcgcgta aggcaatcca aagccattta caaactcaca gttctccaaa
     2041 tagcactcgg tctcacacgt cagatagcgt ttgtacactt ctgaattgac caccgcattt
     2101 ttaatgacga atcctccacc actagctttc cagcacggat ccaagaggag ctcaaggaaa
     2161 aattccccgg agatgctgcg atgattctca ggccaatcaa ctggaaaaga gatgggtcta
     2221 ttctggcgaa tctgctctcg aatccattcc tcaccgtgca ttctcagact ccattaaggt
     2281 gtacctcata gttctaatag aggccttcag tctaaattca ttccatcgta tgtcagccaa
     2341 agagaataat ctggattgtg cacaatatct acgcgagata catggccaag catgttctaa
     2401 acggtggtcc ttgacacttt atatagccaa acgatatata tttataaata taacggaggg
     2461 ccatagatga gtccaaagcc taaaaacacc ccgcatcgag tcctgttgtc tttacccacg
     2521 gacctttaca acactatttc gcgtgtatcc gctgctatgg aaaagccaag agccgaaatt
     2581 attcgcgaat tactagccga acagcaaccg attctcgaac acatggcgac gatgttggaa
     2641 caggcgaaag caggcaaatt ggaccaggct atgaagggat ggcagaaaat gacgggggag
     2701 gcattgaaag gcctgggcgt gctcatgtct cccccagata tcaagaaatc gaagccgaag
     2761 gcgtaagaga gtgcaatctt ctaggcaaac acaatcattt cgagctgaaa gggggtgagg
     2821 aaaatgtcag agcaagtgct taaacgactg tattccgtga gggaggcggc tcgctacctg
     2881 ggcgtgagcc cttggtcggt tcgcaatctt cagaaattac ggactttgcc ctgtgtgcgg
     2941 caaggccgac gggtgctgtt cgatattcat gatttggaac ggcacatcga gaaccacaaa
     3001 acggaggtcc atgatgggca tgatttatcg ccgcaagaaa cgtgatccga ccacaggtaa
     3061 actagaggaa caaggcccct actggatgaa gtattacatc gagggccgag cgattcagga
     3121 aagcacgaaa gtttttgaga aggagcaagc caagaagatt ctcaaggtga aagagggaga
     3181 agtggcggcc ggaatttatc gagggccgag tattgatcga atcaggtttg aagacctggc
     3241 ggcattggtg cagcaggact accagatcaa ccggcgaaaa acggttcgac ggatcacaga
     3301 gtatcagaaa cacttggaac cgtattttcg taaagcccga gtgtcttcta tgaccacgga
     3361 acggatcaaa gcttacatca tcaaacgaca ggagcaaggg gcggctaacg gaacgatcaa
     3421 ccgggaaatc ggatttctta agcggatgtt ccgcctaggc taccaacaaa ctccccagct
     3481 cgtcgctcgc gtgccccaca ttccccagct caaagagcac aacatacgct ccggcttctt
     3541 cgagcatgag gatttcctcg ccttgcgcgg tgccctcccc gactatgccc aagtcccagc
     3601 tacgctggcc tactacagcg gcatgagaat gggagaggtg tactctctcc aatggaggca
     3661 gattaactgg acggagggga aactttttct gaaggcccag gataccaaga cgaacacacc
     3721 acgggttctc tatttgaccg gggaccttta tcgagtgtta ttggcctgga aaacccgttg
     3781 cgacaagaag tggccggcct gtcagtggat ctgccatcga ggcggcattc gcctgcagag
     3841 cttcaaacgc tcctggtggg aagcctgtga agccgtagga ttagggcaca gggtgaagga
     3901 tgagagaaag ggacggctcg tctgggatgg gaagagacct cacgatttcc ggcgtacagc
     3961 ggtgagaaat atgatccggg ccggagtacc ggagaagatc gccatggcca tttctggcca
     4021 caaaaccaga agtgtctttg accggtacaa catcgtgaac gaacgggacc tcgaacaggc
     4081 tgcacgatca ctcacggcct acttcgaaaa gcagacggtt acacttgcgg ttacactggc
     4141 agaattgagg ggtgagagca gcggtgtcgc aaaccgccaa caggttgaaa cgtcggggat
     4201 atttctggag ccggcgagtg gaatcgaacc accgacctgc ggtttacgaa ttcgagtgaa
     4261 tcccctgccc ctattgtatt tcccaatatt cagtctgcaa acgacggcaa attagcgcaa
     4321 cccctccgca attccgcaac cagacgcaac caggttcccg aatccataca ggacgatctt
     4381 cccccaaaaa aggatgagcg tcccaactgt tcttaactca cgtgccgtgc cgtgtgctca
     4441 accgtgcgcg aaggccgaaa gggccagaag ggttggtcgg tatgcatcac atcattgaag
     4501 atggggccga acgtgaacat ctggcggggt gctcttcgtg tcgaaacggg gaaaccgtgc
     4561 cgttcgactg ttcgatcaag ttgcgcggcg agatcgaacg tcgagcagcc ttctatggcc
     4621 gaacgtggga cgaggagctg aaccatatcc tagcagtcgc ctatgaacag cgtgaaccgg
     4681 atgcaggtga tgcggatatg gagagacggc tgaacgaact ccggcgccgt ggagagttac
     4741 cgagccagaa gcggtcgaaa gtcgagattc gccttgcctc catcgatcga ttgccggacg
     4801 gcgttgacgt gcctcgccct accaccgtca aagagctctg caatatctac cttgagtaca
     4861 aactgaaggg ccggcgctcc tatgccaacg ttcgccgaac gatccggcac acgatggaac
     4921 agaccggctt tgctgatgtg aaactccgcg acctgacgcc aggcgtgatc aagcgatggc
     4981 atgccgggat ggcctcgcga catgtgacgg gaaacaacgg actgaagacc ctccgttccg
     5041 cctatcggtt ctgccggagt ttggaattac tgaatgagga tccgaccgcc ggtatcaaac
     5101 ggttcaggga agatgcccgc gaacgctatg tgacgcccag cgaaatgccg cgcctcatgc
     5161 atgcgctgga gagcgcaccc ctccgagcac aaacgttctt cctcacgata ctttttacgg
     5221 cctctcggcc tggagaagtt gccggcatgc agtggaagga tctcgacttt gtgggtatgc
     5281 ggtggcgcaa gccgaccagt aagaccggac gctcacaaga ggtcgtgttg aatccgatcg
     5341 tctaccgatt gctccgccag ctacctcagg tttccgacta tgtgtttccg ggtcaggacc
     5401 tttcgaagc
//
LOCUS       DAORQN010000093         5367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_623_length_5367_cov_10.066077, whole genome shotgun sequence.
ACCESSION   DAORQN010000093 DAORQN010000000
VERSION     DAORQN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_5367_cov_10.066077"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..781
                     /locus_tag="PK614_06265"
     CDS             329..781
                     /locus_tag="PK614_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRB81852.1"
                     /translation="MSQHHRKLLCAPLILAAYTLLSGCATIVHLGGNEELNVSSEPAG
                     AKVVIDGTERGLTPLATKVERKKDHAVVLTKEGFEENQSRVESHLSWWVAGNVILGGL
                     VGILVDVLSGGGYTIEPDAVAVTLKPIEGVATSAAPSLSSLTQSPPIP"
     gene            complement(815..2773)
                     /locus_tag="PK614_06270"
     CDS             complement(815..2773)
                     /locus_tag="PK614_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRB81853.1"
                     /translation="MMPGLRSSAGALFASLLPIVPILFPLVVIFLLATSVSAEDPPTP
                     YLTQLLQQARQARLADAREWHLLLHYRANLFGGYTSEQDDPGFFLAPEGKTDPQAELD
                     ATLRQFFSPELVGRSKQPAQCAFVARYAWLGEQLRFDPIQLPPVPCERFERWIEEFHA
                     RSVTLVFPSAFMNNPASMFGHTFLRIDQEGQTAQTRLLAYTINFAADVPPDEGLAYPV
                     RGIFGGYRGQFSTVPYYLKVQEYRDIENRDIWEYRLNLDDRQIRRLLMHAWELGNASF
                     DYFFFKENCSYHLLSLLEYADPSLHLIERFRFWTIPADTVRALAVEASLVQEIAFRPS
                     RVTLIRRKREHLTDSEHRLVKRLVGDASAAQSEAVRSLPLSRQAFVLDVASDYVRYKA
                     ERDEAQAATARDHNRQILTARSLLRIPSEDLSIAPFAMQPELGHKTSRASLGAGWRND
                     DSYEEVGVRMAYHDLLDPEPGYTPDAQIEVGSISVRHYNRADQTRIERATLLNVLSLS
                     PIDSLFHAPSWKLNIGMQTISHRECQLCSNWNFNGGIGAAAETHMLRREVWFAFAEVE
                     GNYSRAYEERHRVGGGASVGFYADLAERWRLLASTSYLRYALGERTDDVRWSVGQRYT
                     LAQNWAFRLEYSHRDHDNDVLFTVQAFF"
     gene            complement(2773..3282)
                     /locus_tag="PK614_06275"
     CDS             complement(2773..3282)
                     /locus_tag="PK614_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRB81854.1"
                     /translation="MLRQLIVALGVVAVASQAGLAMAANPDTGPGCGLGKLAWADYKN
                     QKNIAPQVMMATTNGTFGSGTFGISSGTSGCTNDGQVMADQKTTMFALLNFENLTQEM
                     AQGKGEHLASLASLMGIPDEQHPAFFALTQERYTALVQDGETSSVALVKTLNDAVSKS
                     PVLAQAVSR"
     gene            complement(3404..3889)
                     /locus_tag="PK614_06280"
     CDS             complement(3404..3889)
                     /locus_tag="PK614_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRB81855.1"
                     /translation="MRIGVVSDTHGLFDRALLRHFSGVDHILHAGDIGKRGVIEQLTQ
                     IAPVTAVSGNVDGFEASGFPVEQVLDLAGYRVALYHRLYEGGRLTRDGTGFLTRTHPA
                     ICVYGHTHQPKVEWQEGVLLFNPGSAGPKRFHLPRAVGLLVLDSGTIDARHIVLADRA
                     E"
     gene            complement(3903..5036)
                     /locus_tag="PK614_06285"
     CDS             complement(3903..5036)
                     /locus_tag="PK614_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB81856.1"
                     /translation="MNPTPNALCFGDGLPGAGAPCYVTVSADGLIMAPVGTSPFVEEA
                     IAFTAMSVEAGGLDHDQLVVSWGAGASVRTLYLKDPALIIAFRRAAPSALMAHLERAA
                     EQVRRARHSHRVLWGSAVGVVIGLGLMVWFGSDLIVEWAVARIPVQWEQKLGETVYQD
                     FLSKETVLKDGPAVSAVQEMTQRMTAKIPNNPYTFQVSVVQSPVVNAFALPGGYVVVF
                     TGLMKKAESGEEVAGVLSHELNHVLQRHGMERLVKMMGLAAVVSILVGDQQGLIGLAR
                     QLGLELATLKFGREQETEADVTGIRLLSDARIAPDGMIRFFERLSEKDKERVELFSSH
                     PMSAARAGRLKAELAALPKQTPEPFTFEWKGVQDSLGASELKK"
     gene            complement(5174..>5367)
                     /locus_tag="PK614_06290"
     CDS             complement(5174..>5367)
                     /locus_tag="PK614_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRB81857.1"
                     /translation="SHLVAWTSTTTYNIEKASSGWIASLTSGEGLVCRFRGPGVVYIQ
                     SRNPGSFGNWIRQFIPVSE"
BASE COUNT         1203 a         1689 c         1440 g         1035 t
ORIGIN      
        1 cgggagttga atctcagcag atttgaccaa cacatggtgt agacgaaagg ctggatcatg
       61 gcttttgcag tgtgtatcag ttcggaaatg acggtgggct gctcaatctt cacgcctcac
      121 tcggtcggaa aataactcgg cagaccttct cctcttctgc aattcctaca gccaccactt
      181 taaaggaagg ccaaatcctc tgagaaggtc atttcttgac gccataccac tggatgctaa
      241 ggtttaaata tcggctgtat gcccgtctcc tggaagatgg cctaggctta atagaacctc
      301 catcacttac cattacagag gaacaatcat gagtcagcat catcgtaagt tgctatgtgc
      361 tccgctcatc ctcgctgcat acaccttact ttccggctgc gccacgatcg ttcacctggg
      421 tggcaacgaa gaactcaacg tttcctcgga accggccggc gcgaaagtcg tcattgatgg
      481 gacggaacga ggcctcacgc ctctggccac caaggtcgag cgcaaaaagg accatgcggt
      541 ggtgctgacc aaagaaggat tcgaggagaa tcagtcgaga gtcgaaagcc acctttcctg
      601 gtgggtagcg ggaaatgtga ttctaggggg attggtcgga attctggttg atgtcttgag
      661 cggtggcggg tatacgatcg aacccgacgc ggtggccgtc acgcttaagc caatcgaagg
      721 cgtggccaca tccgcagcgc cctccctctc cagtctgacc cagtcgccgc ccattccgta
      781 atctccgaaa tttccagtcc gccgtacagg caagctaaaa aaatgcctgc acggtgaaca
      841 gtacatcatt gtcgtgatcg cggtgactgt actccaggcg aaacgcccaa ttctgcgcga
      901 gtgtataccg ctggcccacc gaccagcgca catcgtcggt cctttcaccc aacgcatacc
      961 gcaagtagct ggtcgaagcc agcagccgcc accgctcggc cagatcggca taaaagccga
     1021 ccgacgctcc tccgccgacc cgatgcctct cctcataggc tcgactgtaa ttcccctcca
     1081 cctcggcaaa cgcaaaccat acttctcggc ggagcatatg tgtttccgcg gccgccccga
     1141 tgccgccgtt gaaattccag ttactgcaca actggcattc gcgatggctg attgtctgca
     1201 tgccaatatt taacttccac gacggcgcat gaaacagact atcgatcggc gaaagcgaca
     1261 gcacgttcag cagggtggcc cgttcgattc ttgtctggtc cgctcgattg taatggcgca
     1321 cactgatcga gcccacttcg atctgcgcat ccggggtata gccgggttcc ggatccagca
     1381 gatcgtgata cgccatccgc acaccaacct cttcgtagct gtcgtcattg cgccaacctg
     1441 cccccagact tgcgcgcgat gtcttgtgcc ccagctccgg ctgcatggcg aagggagcaa
     1501 tcgagagatc ctctgacgga attcgcaaga gactccgtgc ggtcagaatc tgccggttgt
     1561 gatctcgagc cgtcgccgcc tgcgcctcgt cgcgctcagc cttgtaccgc acatagtcgg
     1621 acgcgacatc cagcacgaac gcctggcggg agagtggcag actccgaaca gcctccgatt
     1681 gagcggccga cgcatcgcct accaatcgct tcaccagcct atgctccgag tccgtcaagt
     1741 gttctcgttt acgacgaatg agcgtcacac gggacgggcg aaaagcaatt tcttgtacga
     1801 ggctcgcctc gacggcaagc gctcgaaccg tgtcggccgg aatagtccag aaacggaacc
     1861 gttcgatgag gtgcagcgag ggatccgcat actccagcaa cgacaacagg tggtacgaac
     1921 aattttcctt gaagaaaaaa tagtcaaacg acgcgttccc aagttcccag gcgtgcatga
     1981 ggagccggcg gatctgccga tcgtcaagat tcagtcgata ctcccagata tcgcgatttt
     2041 cgatgtcccg atattcctgc accttcaggt aataggggac ggtagagaac tgcccccggt
     2101 agccgccgaa aatgccgcgc accggatagg cgagaccttc atccggaggg acgtcggccg
     2161 caaagttgat cgtataggcc agcaaacgag tctgagccgt ttgtccttct tgatcgatgc
     2221 gcaagaaggt gtggccgaac atggacgccg gattgttcat gaaggccgac gggaacacga
     2281 gcgtgacgga tcgagcatga aactcctcga tccatcgttc gaaccgttcg cacggcacgg
     2341 gtgggagctg aatcgggtcg aatcgcagtt gctcccccag ccaggcatac cgcgcgacaa
     2401 acgcgcactg agccggctgc ttggagcgac cgaccaactc cggcgaaaaa aactgtctca
     2461 gcgtcgcatc aagttccgcc tggggatcgg ttttcccctc cggcgcgaga aagaacccgg
     2521 ggtcgtcctg ctcactcgtg taaccgccga acaaattggc acgatagtgc aacaggagat
     2581 gccattcgcg cgcatccgcc aatctcgcct ggcgggcctg ttgaaggagt tgcgtcaaat
     2641 aaggcgtagg cggatcttcc gccgagacag aagtcgcgag caaaaaaatg actactaaag
     2701 gaaacaggat gggcacgatg ggaagcagag aggcgaacag ggctccggcc gatgaccgga
     2761 gccctggcat cattagcggc tgacggcttg agcgaggaca gggctcttcg acaccgcgtc
     2821 attcagcgtc ttcaccaacg ccaccgacga ggtttcaccg tcctgcacga gcgccgtgta
     2881 gcgttcctgg gttaacgcga aaaacgccgg atgctgttca tccggaatcc ccatgagcga
     2941 cgccagggag gccaaatgct cacccttgcc ctgcgccatc tcctgcgtca aattctcaaa
     3001 gttcaggagt gcaaacatgg tggtcttctg atccgccatg acctgcccat cgttcgtgca
     3061 cccggacgtc ccggagctga tcccgaatgt gccgctgccg aatgtgccat tggtcgtggc
     3121 catcatgacc tgcggcgcaa tgttcttctg attcttatag tctgcccacg ccaacttccc
     3181 gaggccacaa cctggtccgg tatcaggatt cgctgccatg gccaacccgg cctgggacgc
     3241 caccgccacc acacccaacg caacgatcaa ttgtctcagc ataagtcctc ctcctttaca
     3301 caagtgaatg actccgcccg atggtcccca tgaatgacct gctcattata gccaacgctc
     3361 cccaccatgt agagcaaaaa atcctccaag cggactggcc acctcactcc gcccgatcgg
     3421 ccagcacaat gtgccgcgcg tcgatagtcc cggaatcgag cacgagcaga cccactgcac
     3481 gcggcaagtg aaaccgtttc ggtccggcgg acccgggatt gaacaacaat actccctcct
     3541 gccattccac cttcggttgg tgggtatgtc cgtagacaca aatcgccgga tgtgtccgcg
     3601 tcaagaatcc agtcccgtcg cgggtgagtc gccccccttc atacagccgg tgatagaggg
     3661 caacccgata tcccgccaga tcgagcacct gctccaccgg aaacccgctc gcctcgaacc
     3721 catccacgtt gccggatacg gctgtgaccg gagcaatctg tgtcagctgc tcgatgacac
     3781 cacgcttgcc gatatccccg gcatggagga tatggtcgac cccggaaaag tgacggagta
     3841 acgcccgatc gaacagtcca tgcgtatccg aaaccactcc gatccgcatc gcagctccgc
     3901 gattattttt ttagttcgga cgcgcccagt gaatcctgaa cgcctttcca ctcgaaggtg
     3961 aacggctcgg gggtctgctt gggcaatgcg gccagctccg ctttcaaccg cccggcgcgg
     4021 gccgcgctca tggggtgaga ggagaagagc tccacacgtt ctttgtcttt ttccgagaga
     4081 cgttcaaaaa accggatcat gccgtccggg gcgattcgcg catcagacaa gaggcgaatg
     4141 ccggtgacat ccgcttccgt ctcctgctcg cggccgaact tcaacgtggc caactccaag
     4201 cccaattgcc tagccagacc gatcaggcct tgctgatctc cgacgagaat actcaccacc
     4261 gcggctaacc ccatcatctt cacgaggcgt tccatcccat gccgctgcaa cacgtggttc
     4321 aactcatgac tcaatacccc ggccacttct tcgccgctct ccgccttctt catcaagccg
     4381 gtgaacacca ccacataccc ccccggcaac gcaaacgcgt tcaccaccgg gctctggacc
     4441 accgacacct ggaacgtgta ggggttgttg ggaatcttgg cggtcatgcg ctgagtcatt
     4501 tcctggaccg cgctgacagc cgggccatcc ttgagcaccg tttctttgga caagaaatcc
     4561 tggtaaaccg tttcgccgag cttttgctcc cactgaacag gaatcctggc gaccgcccat
     4621 tcgacgatca gatccgaccc gaaccacacc atcaatccca gtccgatcac cacccccacg
     4681 gcgctgcccc acaagacacg atggctgtgg cgcgcgcgac gaacctgctc ggccgcccgc
     4741 tcaagatgcg ccatcaacgc agacggagcg gcgcgacgaa acgcgatgat caatgccgga
     4801 tccttgagat acagcgtccg gaccgatgcg ccggctcccc acgacaccac cagctgatca
     4861 tgatccagcc cccccgcctc aacggacatg gccgtgaatg cgatggcctc ctcaacgaac
     4921 ggagaggtcc cgaccggagc catgatcaga ccatcggccg agaccgtgac atagcaaggc
     4981 gcacccgccc ccggcaggcc atcaccaaaa cagagcgcgt tgggagtagg gttcatcggg
     5041 ttcgatcttg cggctgatca aaatggttgt gaacgaggcc gcaggcgaac gcaaaccgga
     5101 ggcgtaccct cagcggtacg ttgaggattt gtcgtgagcc gagaacgaag tgcacgggca
     5161 ttttcatcag ccactactca gagaccggga tgaattgtct gatccaattc ccgaagcttc
     5221 caggattgcg actttggatg tagaccacgc cggggccacg gaagcggcac accagccctt
     5281 cacctgacgt caggctggcg atccagccgg aggaggcctt ttcgatattg tacgtggtgg
     5341 tggaagtcca ggcgacaaga tggctgt
//
LOCUS       DAORQN010000094         5358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_627_length_5358_cov_8.188573, whole genome shotgun sequence.
ACCESSION   DAORQN010000094 DAORQN010000000
VERSION     DAORQN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_5358_cov_8.188573"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="PK614_06295"
     CDS             <1..2063
                     /locus_tag="PK614_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRB81858.1"
                     /translation="QDGLVAADQLKPGSRLRERNLHKLPASMPLSPLLHELQGRLSAE
                     SARLSSYRDAQPQLKQFLDQLARYLGLVGLTALFVGGIGVALSIQAFVREKLQSIAIL
                     KTLGADTKTIIYSYLGQAVGLGVLGSAVGIGIGLLLQAVLPQAVSTLLATDVLQQVEF
                     TSVLSWTALAPLSKGLGLGVLTTLLFSLWPLLTIRTIKPAVIFRREVEGAIRPGVRPE
                     TSVWGRAAQVVTADPVRAVTAAGIGLGLAGLSVWQAGSLMVGGLFIGGLLVAVVALTV
                     TAKALLLGIRVLPSPRALSLRQALGNIQRPGGQTLGVMVSIGVGVMVILAIALLEQAL
                     VRQVGENRPNDSPTFFFVDIQPDQAEGFSALIHRRTGDVAPDLTPLVRSRLHAINGQT
                     VTVERESEQEDQPGQSKEEKRKNWYVNREYVLTFLDQLPKDNRIVKGAWWKPGQVFTR
                     PQVSIEEEAAKSLGIDVGTTLDLNIQGTIMRAEVSSIRKVEWGNFSTNFYMIFSPGAL
                     DGAPMTYVATVRVSLQDEVALQSAVVAAFPNVTAINIGEVLSSFARVLDRLSLAIRAV
                     ALFCLLAGALVMAAALAATRYRRLYEAVILKALGATRALIARSFAAEYAVLGCVAGVI
                     GVGLASLFSWAILRYILELPWSLEPSLLGIGLGCTVLLTLIVGFLSTYRLLGQPPLTV
                     LRHE"
     gene            complement(2094..4871)
                     /locus_tag="PK614_06300"
     CDS             complement(2094..4871)
                     /locus_tag="PK614_06300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRB81859.1"
                     /translation="MTGDRTDPPVTRRHAALLATRVLTKAVVWLGAWHGATSWFFVPA
                     AWSHSNPPHNHRQPKTHTMGSSMTTHDPYRLPRHVLPSHYDLRIEPDLQAHSFTGHEV
                     VTLTVTEPTTEILLNATELEVSTATLSGEGTPPRTGTVRMDEEHQRCYITFPSVIQPG
                     AWKLSLAFRGTLNDKLRGFYRSSYKDEQGHSHSLAATQFEATDARRAFLCWDEPQFKA
                     VFAVTLAIDPALTAISNTRIVDDRQEGGKRVLRFAESMKMSTYLVAFIVGKLDATAPT
                     MARQTPVRLWSVPSKQHLTPFGQEIAVYSLNFLADYYGIPYPGDKLDLIAIPDFASGA
                     MENLGAITFRETALLLDQRTATHAEQGRIADVVAHENAHMWFGDLVTMAWWNGLWLNE
                     AFATFMEMLVVDAWKPEWERWTAFGVSRAAALSVDGLLSTRPIEFPVRAPKEAEAMFD
                     VLTYEKGASVLRMLEQHIGPTVFRDGVRHYLTTHAYGNAETTDLWVSLAHASQQNVPA
                     LMNEWIFSPGYPLLSLAIDSSSTLTLTQRRFTYAEGSTAASSGTPAQLWQVPIQLRIH
                     TARGAETRRVMLSDQENRIPLPKDWTSVLANEGGHGFYRVRYSTTLLNGLQQTGLQTL
                     APVERFNLLNDTWASTIAGMVSPADYLSLTEHFRGEQDPHVWAVMLGSFSTMNHLLSE
                     EDRPLLAAFVRNRLTPTFQDLGWTPRTDERDLVKELRGDVIRALGTLGRDPSVQTQAL
                     EAYTDLQQQTRPIDPNVIPALVSILAFTGDAARYEEFLNRFHKAPTPQEERRYLFSLA
                     AFRIPELLERTLAKTLTDEIRTQDAPFLVSSLLHNVYIREKAWEFVKTNWERMDRQFP
                     KSGLRRMCGGITGLSTPELEQDVRAFFTSRKIDLGGKTLEQYLEQLHIAVRFRERDRD
                     AIRAVLARVAM"
     gene            complement(4868..>5358)
                     /gene="rpiA"
                     /locus_tag="PK614_06305"
     CDS             complement(4868..>5358)
                     /gene="rpiA"
                     /locus_tag="PK614_06305"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HRB81860.1"
                     /translation="PLIESDNAWMIDVAIDGADQVDPALNLVKGGGGALLKEKIVAAA
                     AKRFIVMVDHTKLVPALGGSFPLPIEVVPFGWGSTARNIEQASGGKAVLRERNGVVFQ
                     TEAGHVILDLHMPKIEDPATLEIELNQIPGIVETGLFIGRTSILIVGHAQGADVTLAA
                     ES"
BASE COUNT          941 a         1498 c         1743 g         1176 t
ORIGIN      
        1 cccaagacgg tctcgtggct gccgaccaac tcaaaccagg aagtcgtctt cgcgaacgta
       61 atctacacaa gttacccgct tccatgcccc tgtctccgct gctccatgag ctccaggggc
      121 gcctttccgc ggagtccgca cggctgtctt cctatcggga tgctcagcca cagctcaagc
      181 agtttctcga tcagctcgcc cggtacttgg ggctagtggg attgacggcg ttgttcgtcg
      241 gcgggattgg cgtggccttg tcgattcaag cgttcgttcg agaaaaactc caatcgattg
      301 cgatcctcaa gacattgggc gcggatacga agaccatcat ctattcgtat ctgggacaag
      361 cagttgggtt gggggtgctg ggcagcgcgg tcggtattgg gatcgggctc ctgttgcagg
      421 ccgttttgcc gcaggcggtc tccaccttgc tcgcgacgga tgtcctgcag caggtcgagt
      481 ttacgtccgt gctgtcttgg acagcgctgg cgccgctctc gaagggcttg ggcttgggtg
      541 tgctgaccac ccttctgttc agtctgtggc cgcttctgac gattcgaacc atcaagcctg
      601 cggtcatctt caggcgtgag gtggaaggtg caatccgccc cggtgttcgg cctgagacat
      661 cggtgtgggg ccgcgcggca caggtcgtca cggcggatcc tgttcgtgcc gtcacggcgg
      721 ccggcattgg gcttggtttg gcgggcctct cggtgtggca ggccgggtct ctgatggtcg
      781 gagggctctt tatcggcggc ctcctggtcg ctgtggtggc gttaaccgtg accgcgaaag
      841 cactcctcct cgggatacgt gtgttgccca gcccgcgtgc gctttctctg cgacaggcgc
      901 tcggcaacat tcaacgtccc ggagggcaaa cccttggggt gatggtttcc atcggtgtcg
      961 gggtgatggt gattctcgcg attgcgttgc tggaacaggc cttggtgcgt caagtcggcg
     1021 agaaccgacc gaacgattca ccgaccttct tttttgtcga tattcagccg gatcaggcgg
     1081 agggcttttc cgccttgatt catcggcgca ccggcgatgt ggctcctgat ctgactccgc
     1141 tggtgcgctc tcgattgcac gcgatcaatg gccaaacggt cacggttgag cgggagtccg
     1201 aacaggaaga ccagcccggt cagtcgaaag aagagaagcg gaagaattgg tatgtgaatc
     1261 gtgagtatgt gctgactttt ctcgatcaac tgcctaaaga caataggatt gtcaaaggtg
     1321 cctggtggaa gccagggcaa gtttttacgc gaccgcaggt gtctatcgaa gaagaagcgg
     1381 caaagagtct tggcattgat gtgggcacca ctttggacct caatatccag gggacgatca
     1441 tgcgggctga ggtgagtagt atcagaaagg tcgagtgggg aaatttctcc acgaatttct
     1501 atatgatctt ttcaccggga gcattggatg gggcgccgat gacctatgtc gcgacggtgc
     1561 gggtgtccct gcaggatgag gtggcgctgc agtcggccgt ggtcgccgca tttcccaatg
     1621 tgacggcgat taacatcggc gaggtcttaa gcagcttcgc ccgggttctc gaccggctgt
     1681 ccctggccat ccgcgcggtg gctctgtttt gcctgttggc cggggcactc gtcatggcgg
     1741 cggctctggc ggctacgcgg tatcgtcgtc tctatgaggc cgtgattctc aaggctctcg
     1801 gcgcgacccg tgcgttaatt gcccggtcgt ttgcagcgga atatgcagtg ctggggtgcg
     1861 tggcgggggt gatcggcgtg ggcctggcca gcctcttctc gtgggcgatt ctgcgctaca
     1921 ttctggagtt gccctggtcg ctggagccgt cgctgctggg catcgggctg ggttgcactg
     1981 tgttgctcac cctcatcgta gggttcctca gtacctaccg cctgctcggg cagcctccgt
     2041 tgactgtgct tcggcacgag tgacggccgg ctggatgtac tcggccgttc ccactacatc
     2101 gcgacgcgtg ccagcacggc gcgaatggcg tcgcggtcgc gttcccggaa tcgtacggcg
     2161 atgtgcagct gctccaggta ctgctccaga gtcttcccgc ccaggtcgat cttccgggat
     2221 gtgaagaacg ctctgacgtc ttgctccaat tccggagtcg aaagaccggt gatccctccg
     2281 cacatgcggc gcaggccgct cttgggaaac tgccggtcca ttcgctccca attagtcttc
     2341 acaaactccc aggctttttc acgaatgtac acattgtgca acaggctgct gacgaggaag
     2401 ggcgcatctt gggtgcggat ctcgtcggtc agggttttcg ccagggtgcg ctccagcagt
     2461 tcgggtatcc ggaaggccgc gagtgaaaag agatagcgac gctcttcctg cggggtgggg
     2521 gccttgtgga agcggttgag aaattcctcg tagcgagccg cgtcgcctgt gaaggccagg
     2581 atggaaacga gcgcggggat cacattgggg tcgatcggtc gagtctgctg ctgcagatca
     2641 gtgtaagctt ccagcgcctg ggtttggacg ctggggtcgc gacccaatgt gcctaaggcg
     2701 cgaatcacgt caccgcgcag ttctttgacg agatcgcgct catcggtacg cggtgtccat
     2761 cccagatcct ggaatgtcgg ggtgagtcga ttgcgcacaa aggcagccaa cagcggtcga
     2821 tcctcctcgc tcagcaggtg attcatggtg gagaacgacc ccaacatcac tgcccagaca
     2881 tgcgggtcct gttcgccacg gaagtgttcc gtcaagctga gatagtcggc gggtgacacc
     2941 atgccggcga tggttgaggc ccaggtgtcg ttcaggagat tgaaccgttc gacgggggcc
     3001 agggtctgga ggccggtctg ttgcaggccg ttgaggagcg ttgtgctgta tcgaacgcgg
     3061 tagaatccgt gcccgccctc gttcgccagt actgatgtcc aatccttcgg cagcgggata
     3121 cggttctcct gatcgctgag catcacacgc cgggtttcag cgccccgcgc ggtgtggatg
     3181 cgcaactgaa tcggaacctg ccagagttgc gccggtgtcc cggaagatgc cgccgtagag
     3241 ccttcggcgt aggtgaagcg acgctgtgtg agcgtcagcg tggaggatga gtcgatggca
     3301 agcgagagca agggatatcc cggcgagaag atccattcgt tcatgagtgc cggaacattc
     3361 tgttgtgacg catgcgcgag cgagacccac aggtcggtag tctctgcgtt cccataggca
     3421 tgcgtcgtca gatagtggcg gacgccgtct ctgaataccg tcggaccgat atgctgttcc
     3481 agcatgcgca ggacggaggc tcccttttca taggtcagca cgtcgaacat ggcttcggct
     3541 tctttgggcg cgcgcacggg gaactcaatc ggcctcgtgc tcagcaggcc gtcgacagac
     3601 agagcggcag cgcgtgagac gccaaaggcc gtccagcgtt cccattccgg tttccaggca
     3661 tcgactacca gcatttccat gaaggtggcg aaggcctcgt tgagccagag cccgttccac
     3721 caggccatgg tcacgaggtc gccgaaccac atgtgggcat tctcgtgcgc gaccacatcc
     3781 gcgattcgtc cctgctccgc atgtgtggcc gtgcgctggt ccagcagcaa cgctgtttca
     3841 cggaatgtga tcgcgcccag attttccatc gccccggaag cgaagtcggg gatggcgatg
     3901 aggtccagtt tgtcgcccgg ataggggatg ccgtagtagt cggcgagaaa attgagcgag
     3961 tacacggcga tttcctgacc gaacggggtc aggtgttgtt tgctgggcac ggaccagagg
     4021 cggaccgggg tctgccgggc catggtggga gctgtggcgt cgagtttccc gacgatgaaa
     4081 gccacgagat atgtggacat cttcatggat tccgcgaagc gcagcactcg cttcccgccc
     4141 tcttgtcgat cgtccacgat ccgggtattc gagatggcgg tcagtgcggg atcgattgcc
     4201 agggtgacgg cgaagacggc tttgaattgc ggttcatccc aacagaggaa ggcccgccgc
     4261 gcatcggttg cctcaaactg ggtcgcggcc agactgtgcg aatggccctg ttcatccttg
     4321 tagctactgc gatagaaccc gcgcaattta tcgtttagtg tgccgcgaaa cgccaggctt
     4381 aatttccagg cgccgggttg aatgaccgac gggaaggtga tgtagcatcg ctggtgctcc
     4441 tcgtccatcc gtacggtccc ggtgcgcggc ggcgtccctt caccggacaa tgtcgctgtc
     4501 gagacctcca attcggtcgc attcaggaga atctcagtag tcggttcggt caccgtcagc
     4561 gtcacgacct cgtggccggt gaacgagtgt gcctggaggt ccggctcgat acgcaagtcg
     4621 taatgagacg ggaggacatg gcgcgggagt ctgtagggat cgtgagtcgt catagatgaa
     4681 cccatagtgt gtgtcttcgg ctgtcgatgg ttgtgtggcg gattcgagtg gctccaggcc
     4741 gcgggcacga agaaccagga ggttgcgccg tgccaggctc ccaaccacac cactgctttg
     4801 gtgaggactc gtgtggccag caacgccgca tgtcgccgag tgacgggagg gtcggtccgg
     4861 tcgcccgtca tgactccgct gcaagggtga cgtcggcacc ctgtgcatgg ccgacgatca
     4921 ggatgctggt gcgtccaatg aacagtccgg tttcaacgat gccgggaatc tggttaagtt
     4981 ctatctcgag ggtggccgga tcttcgatct tgggcatgtg cagatcgagg atgacgtggc
     5041 ccgcttcggt ctgaaacacg acgccgtttc gctcgcgcaa gacggctttg cctcccgagg
     5101 cttgttcgat gttccgtgcc gtgctgcccc atccgaacgg cacgacttca atcggcaacg
     5161 gaaaactgcc gccgagcgcc ggcacgagtt tcgtgtggtc gaccatcaca ataaagcgct
     5221 tggctgcggc tgccacaatt ttttccttga gcaacgcgcc tccgccgcct ttcacgaggt
     5281 tcaaggcggg atcgacctgg tcggctccgt cgatggccac atcgatcatc catgcgttgt
     5341 cggactcgat gaggggaa
//
LOCUS       DAORQN010000095         5357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_628_length_5357_cov_4.731611, whole genome shotgun sequence.
ACCESSION   DAORQN010000095 DAORQN010000000
VERSION     DAORQN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_5357_cov_4.731611"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PK614_06310"
     CDS             complement(<1..682)
                     /locus_tag="PK614_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRB81861.1"
                     /translation="MYLKQYAKTTFARECLGEYCQYLYNGEPCFRTVGNDATFYRPPT
                     PNQLRKYLNQIPEDFEMCFKVWEEITIPSYAKQPRYGSKAGQPNPNFLDAKLFNELVL
                     KPYREAKFEPHTGPFLFEFQRHGMSSEEFCSRLDKFFSQLPKDFMYAVEIRNAGIVGT
                     EYRQVLEQHGVAHVYNHWSYMPPLREQHKRMASFTAPFTVLRLLTPLKMSYEAAKKRA
                     EPYNKIVGE"
     gene            complement(766..>2731)
                     /locus_tag="PK614_06315"
     CDS             complement(766..>2731)
                     /locus_tag="PK614_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB81862.1"
                     /translation="DERPAILEWVFKHYGHQQAAMVANQNTLAPRAALREIAKVHGLP
                     ASEIKKTLNLLHRRADFVEVHSDINVKNWAREVCSALQLRSPWPDILYWSVQLEGHFR
                     NLGLHPGGVVLVPDEIRRYVPVEISASGLPVIQWEKDQTEDAGLVKIDLLGNRSLAVI
                     RDALAAIARNTGRVIAYATWDPLSDPNTNELIRRGETMGCFYVESPATRLLLKKLWAG
                     MPPDRRAASDVFEYLVVVSSLIRPAANTFTEEFVRRAHGKQYTSPHPLLDEVLAETHG
                     IMVYQEDVMKVAVALGGFSVEDGDQLRKVLSKKHKARQLRDYCRQFYEGAQARGVELP
                     VIDAIWAMIMSFAGYSFCKPHSASYAQVSFKSAYLRAHYPAEFIAAVVSNQGGYYSAF
                     AYLSEGRRMGLTILPPDINESVWTYTGAGKAVRVGFMQIKGLQGEMVKQLIAERHTNG
                     PYKSLADFVTRVKPDMAQAKLLIKAGCFDSIAGELTRPALLWRLLASQATTPPSYVPI
                     PEAYSFQKSLAHELTLFGFPLQCHPLDVFREAYKDTHYILAKDLALYIGKKVTLIGWL
                     LTEKIVSTKKGDPMEFMTLEDQTGMYDATVFPQTYRQYCHLLAPNQGYIMTGLVEEQF
                     STVTVTVRTLRLLNAAEIQAPSEALEEMNA"
     assembly_gap    2732..2741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2742..3729)
                     /locus_tag="PK614_06320"
     CDS             complement(<2742..3729)
                     /locus_tag="PK614_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRB81863.1"
                     /translation="MSGIPTLEALCQAARTQGAQYLALTDTNGLYGSIHFLGVAREAG
                     LKPILGGELIHGPHRAVLLAKTPSGYANLCRILSARHCDESFDFIKTVAQYREGLVIL
                     SDTQRALSEWQKESKDDLYVELTPGPSLAEAVAFSRAHQLSPVATARAHFLMPSDYQA
                     HRLLRAIAENTTLSRLKVDQCCAPSQWLMPETQLARACPHVPEALLNTQRIAESCHTD
                     WDFKQTIFPSFRQLSSRAAFETLRSKTYEGAHWRYGTLSAAVTERIEKELTVICEKGY
                     ADYFLVVDEIVRQAPRTCGRGSAAASIVSYCLGITHVDPIKHHLMFERFLNPG"
     gene            complement(3772..4950)
                     /locus_tag="PK614_06325"
     CDS             complement(3772..4950)
                     /locus_tag="PK614_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81864.1"
                     /translation="MDRQIVCFAIPAVEVALAQLHEPRLRTRPVAIAPLNTARALIRE
                     VSSDAARAGLAVGMSVDHARRICPSLQVLAPNPSRVRTADESLLRVVARYAPVWELSQ
                     PGSFLMDVTGTGRLFGPANDIAAKVQQEVLEQYRLDGVAGVGTNKLIAQTAATLIEPS
                     DLYDVRPGSEEVFMSPLPVGTLPGLQRACMRKIVHLLDDLNLCTLGDVAGSPLDALEV
                     AFGDYAGSLSRWAQGIDTVPVLPPTVQPMLEETVLFEPDEIDDPVLIGRSLDALQRLC
                     RSLRTQRRVCGCLTLSIRYTDQIEVTKQQQVQPETCWECDLSPIAVSLFHKCMRRRIR
                     LRALTLSMSGLTAYAEQGLLFEERTPDHRKRERAQKLALALDALKARFGEQTIRYGRS
                     "
     gene            complement(5003..>5357)
                     /locus_tag="PK614_06330"
     CDS             complement(5003..>5357)
                     /locus_tag="PK614_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRB81865.1"
                     /translation="LATVHIELSGVVAAGAPIEPFPQTERVEVPKSMIGRGDTFALRV
                     NGNSMRDEGILPGDVVVVQKQATARNGQTVIALVNGEATIKTSAKPEPWNSIRPMKPC
                     SQLSSSPRTHSISKE"
BASE COUNT         1179 a         1505 c         1500 g         1163 t
ORIGIN      
        1 gttcccccac aatcttgttg tagggctcag cccgtttttt tgcagcttca taagacattt
       61 tcaatggcgt cagaagccga agaactgtaa atggtgcggt aaatgacgcc atgcgcttat
      121 gctgttctct caacgggggc atgtaggacc agtgattgta gacatgggcc accccatgtt
      181 gctccagcac ttgccgatac tccgtgccga caattcccgc gttccgaatt tcgaccgcgt
      241 acatgaagtc tttcggcagt tgactgaaga atttatcaag tcgcgaacag aattcctcgc
      301 tcgacatgcc gtgacgctgg aattcgaaga gaaagggccc cgtgtgcggc tcgaactttg
      361 cttcacggta tggcttcagc actaactcgt tgaacagctt tgcatcaagg aagttcggat
      421 taggctgccc agctttggac ccataacgcg gctgcttagc atagctggga atggtgattt
      481 cctcccagac cttgaaacac atttcaaagt cttctggaat ttggtttaga tacttgcgga
      541 gttggttagg tgtcggtggg cggtagaagg ttgcgtcgtt gccaaccgtg cgaaaacatg
      601 gctcgccgtt ataaagatat tggcaatatt cccctaaaca ttcccgtgcg aaggtcgtct
      661 tcgcatattg cttcaggtac acctgccctt gccaaccttc ataggtccac gtggacgagc
      721 caaatctgac gagaggtgaa agcggcatgg tgcgagagtg tacggctatg cgttcatttc
      781 ctcaagagcc tcgctcggtg cctggatttc ggctgcgttc aagagccgga gcgttctcac
      841 ggtgaccgtc acagtcgaga actgctcttc cacaaggccg gtcattatat agccttgatt
      901 tggtgccagg agatggcagt attgccgata ggtctggggg aatacggtcg catcgtacat
      961 cccggtctga tcctccaggg tcatgaactc catcgggtcg cccttcttcg tcgagacaat
     1021 cttctccgtg agcagccagc cgatgagcgt gactttcttg ccaatgtaca gagccaggtc
     1081 tttcgccaaa atatagtgag tgtctttgta tgcttcacga aacacatcga gtggatgaca
     1141 ctgcaacgga aacccaaaga gtgtcagctc atgagcaagg gatttttgaa acgaataggc
     1201 ttctggaatg gggacgtaac tcggcggcgt cgtggcctgt gacgccagga gccgccagag
     1261 caaggccggt cttgtcagtt caccagcaat ggaatcaaag cagccagctt tgatgaggag
     1321 cttcgcttgg gccatatccg gcttcacccg cgtaacgaaa tccgcgagcg acttataagg
     1381 gccgttggtg tgtcgttcgg caatgagctg ctttaccatc tctccctgaa gtcctttgat
     1441 ctgcatgaag ccaacacgaa ccgccttgcc agccccggta taggtccaga cactttcatt
     1501 aatgtctgga gggagaattg tgagtcccat ccgtcttccc tcagacaggt acgcgaaggc
     1561 ggaataatac cccccctgat tactcaccac ggcagcgatg aactcggctg gataatgcgc
     1621 ccgaaggtac gcagatttga atgagacttg cgcatagctc gccgaatgcg gcttacagaa
     1681 gctatatcct gcgaagctca tgatcatcgc ccaaatcgca tcgatgacag ggagttcaac
     1741 gccacgggct tgcgcgccct catagaactg gcggcaatag tcgcggagct gccgcgcctt
     1801 atgcttcttg ctcaagacct ttctaagctg gtcgccgtct tcgacggaga agcctcccaa
     1861 cgccacggca accttcatca catcttcctg ataaaccatg atcccatgcg tttcggccag
     1921 cacctcatca agcaagggat ggggtgacgt atactgtttt ccatgcgccc gcctgacaaa
     1981 ttcctcggtg aaggtattcg ccgcaggacg aatgagcgag gacacgacga cgagatattc
     2041 aaacacatcc gatgccgctc gcctgtccgg cggcatcccg gcccagagtt tcttgagcaa
     2101 gagccgggtg gcgggtgatt ccacatagaa gcagcccatg gtctctcctc gccggatcag
     2161 ctcattggtg ttcggatcgc tgagcgggtc ccacgtggca taggcgatga cgcgcccggt
     2221 gtttcgcgcg atagcagcca gcgcgtcacg aatgaccgcc agcgaccgat tgcccagaag
     2281 gtctatcttc accaaccctg cgtcctcggt ctggtccttc tcccattgga ttactggcaa
     2341 tcccgaggcc gagatttcca ccggcacgta gcgccggatt tcatcgggca ccagcaccac
     2401 gccgccagga tgaagcccga ggttgcggaa atgcccttca agctggactg accagtaaag
     2461 aatgtccggc catggagacc tgagctgcaa ggctgagcag acttctcttg cccaattctt
     2521 gacgtttata tccgaatgga cctcaacgaa gtcggctcgc cgatgaagga ggttcagggt
     2581 cttcttaatt tcactggcag ggagcccgtg cactttcgcg atctcgcgga gcgccgcccg
     2641 tggggcgagc gtgttctgat tggccaccat ggcagcctgt tgatgcccat agtgtttgaa
     2701 gacccattct agaatggccg gacgttcatc cnnnnnnnnn ntaccaggat taaggaaccg
     2761 ttcaaacatc agatgatgct tgatcggatc gacatgcgtg atgccgaggc agtacgagac
     2821 aatcgacgca gcggcagagc cgcgcccaca ggttcgtggc gcttgccgga cgatttcatc
     2881 aacgacgaga aaatagtcgg catacccttt ctcacagatg accgtaagtt ccttttcaat
     2941 ccgctcggtc acggcagcag acagcgtgcc atagcgccaa tgggctcctt cataggtctt
     3001 gctgcgcaat gtctcgaagg cggcgcgtga ggacagttgc cggaaggaag gaaagatcgt
     3061 ctgcttgaaa tcccagtcag tgtgacatga ttcagcaatc ctttgggtat tgagcaaggc
     3121 ttccgggaca tgaggacacg cacgagcaag ctgtgtttcc ggcatgagcc actgtgacgg
     3181 agcacagcac tggtccacct tcaaacggga cagcgtggtg ttttcggcga tggcccgtaa
     3241 cagtctgtgc gcctgataat cggacggcat caggaaatgc gctcgggcag tagcgaccgg
     3301 tgaaagctgg tgagcccggc tgaatgcgac agcttcggca agcgaagggc ctggcgtgag
     3361 ttcgacatag aggtcgtctt tcgactcttt ctgccattcg ctcaaggcac gttgagtatc
     3421 agatagaatg accagccctt cgcgatactg tgccacggtc ttgatgaagt cgaaggactc
     3481 gtcacaatgc cgtgccgaaa gaatccggca gagattggca tagccggacg gtgtttttgc
     3541 taacaggacg gcacggtgcg gtccatggat cagctcaccc cccaggatcg gctttaggcc
     3601 tgcttcccga gcgaccccaa gaaaatgtat ggacccataa aggccattcg tatcggtcag
     3661 ggcgagatac tgcgctcctt gtgttcgtgc agcctggcaa agcgcttcca aggtcggaat
     3721 gcccgacatg ggagagtagc ttgaatgggt atgcagatga acgaagggca tttaagaccg
     3781 tccgtagcga atggtctgtt cgccgaaccg tgccttgagg gcatccaggg cgagcgctag
     3841 cttctgggct cgctcccgtt tcctgtgatc cggagtccgc tcctcaaaca acaatccttg
     3901 ttcggcgtaa gccgtcaggc cggacatgct gagcgtgagc gccctgagcc ggatacgccg
     3961 ccgcatacat ttgtgaaaga gcgacacggc tatcggggag aggtcacact cccaacacgt
     4021 ctctggctga acctgctgct gcttggtgac ttcgatttga tcggtgtaac gaatgctgag
     4081 cgtcaggcat ccacacacac gtcgctgagt gcgtaagctc cggcagagcc gctgcaacgc
     4141 gtcgaggctt cgcccgatca ataccggatc atcgatttca tccggctcga agaggactgt
     4201 ctcttccaac atcggctgaa cagtcggcgg cagtaccggt actgtatcga tcccctgcgc
     4261 ccaacgggag agcgatccag cataatcccc gaaggccact tccagcgcat caagcgggct
     4321 tcctgccacg tcacccaatg tgcagaggtt gagatcatca agcaggtgaa ctattttccg
     4381 catgcaggca cgctgcaggc ccggcagggt gccgaccggg agcggcgaca tgaagacttc
     4441 ttccgaacca ggccgcacat catagaggtc cgatggctca atcagcgtcg ccgctgtttg
     4501 agcaatgagt ttgttagtcc cgactccagc gactccatcg aggcgatact gttccagaac
     4561 ctcctgttgg accttagccg caatatcatt agccggtcca aagagccgtc cggttccggt
     4621 gacatccatg agaaaggagc ctggctggga cagttcccat acgggagcat accgagcaac
     4681 gacccggaga agtgattcat ccgccgttcg cacgcgtgat gggttcgggg ccagcacctg
     4741 gagcgatgga cagatgcgcc gggcatggtc caccgacata ccgacagcaa ggccagcccg
     4801 tgcggcatca gacgacactt cacgaataag ggcacgagcc gtgtttaatg gagcaatggc
     4861 tacgggccgg gttctgaggc ggggctcatg gagttgcgca agagcgactt caacagccgg
     4921 tatcgcaaaa cagacaatct ggcggtccat gatactgact cactctcgtc tcacttccga
     4981 aggtgacgga tgaccccgac gactattcct tcgatatgga atgcgtccgt gggctggacg
     5041 acaattggct gcatggtttc attggccgga tggagttcca cggttccggc tttgcgctgg
     5101 tcttaatcgt cgcctcgccg ttcaccaggg cgatgacggt ctgcccattc cgggcggttg
     5161 cctgcttctg caccacgacg acatcccccg gcagaatgcc ttcgtcccgc atggagtttc
     5221 cattcacccg cagagcgaag gtatcgcccc gaccgatcat gctcttcgga acctcaaccc
     5281 gttcggtttg cggaaatggt tcgataggcg caccagcggc caccacccca gacaactcga
     5341 tatggacggt cgccaag
//
LOCUS       DAORQN010000096         5354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_629_length_5354_cov_9.261559, whole genome shotgun sequence.
ACCESSION   DAORQN010000096 DAORQN010000000
VERSION     DAORQN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_5354_cov_9.261559"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK614_06335"
     CDS             <1..448
                     /locus_tag="PK614_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRB81866.1"
                     /translation="DSLAVEQRKRLESRRITNRKFDIAVSDRPSLPEEATRQLRVRLI
                     QEEFEELQEAMVAQDLPGVAKELADLLYVVYGTAVSYGLDMDPVFREVHRSNLSKVGG
                     YKRADGKWVKPPTYSPAQVEPLLAAQSSGSLADRSQVDTRMQESES"
     gene            450..704
                     /locus_tag="PK614_06340"
     CDS             450..704
                     /locus_tag="PK614_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81867.1"
                     /translation="MKELYCPACGTPCVRVTSGTNLMEKTLNRLSIFQVRCQLCTARF
                     QARRPGNRQTSQEFDRREYRRLRANFAASLILDQPAVGGG"
     gene            719..961
                     /locus_tag="PK614_06345"
     CDS             719..961
                     /locus_tag="PK614_06345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81868.1"
                     /translation="MGGCTLQASSSLPRGTFVKLIVHAPAGQPDIKVDAAMICSIQPL
                     SIGVKFLEFEPDDKQRMGQLVLELLAHQQAPPRVSA"
     gene            complement(1047..1904)
                     /gene="panC"
                     /locus_tag="PK614_06350"
     CDS             complement(1047..1904)
                     /gene="panC"
                     /locus_tag="PK614_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRB81869.1"
                     /translation="MKIIRATPAMADWSRRLHREGITIGFVPTMGALHEGHRALIRAA
                     RLACDAVVVSIFVNPTQFGPTEDLSKYPRQLRRDQALCRKEGVDVIFAPTVRTMYPTG
                     AQTVVTVPGIARRWEGEARPHHFQGVATVVTKLLSLVRPDKTWFGQKDYQQAALVQQL
                     VKDLNLPGLPIVHPTMREADGLALSSRNVYLSSSERQAAPVLSRALQAGVAAIRAGER
                     NGTRIRRHMLRVVTTEPLATVEYLAVCDPATLEPLSLLNRQAVLLGAIRIGTVRLLDN
                     VLTSFRRKL"
     gene            complement(1901..2464)
                     /gene="folK"
                     /locus_tag="PK614_06355"
     CDS             complement(1901..2464)
                     /gene="folK"
                     /locus_tag="PK614_06355"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRB81870.1"
                     /translation="MATAFIGFGSNLGNRIDFCDRAVTLLGLLPHSRLEAVSSLYETE
                     PLEDGAAPGPSWFLNGVVQIETNIAPKSLLEICREIERALGRDQDNRKGPRTLDLDVL
                     LYDDCILAEPALTVPHPRLHQRRFVLTPLVELDPERRHPVLGQNLRDLLSQLTDPSIV
                     RLLEPQPNSRYGSRPTCSAPPTAGPQA"
     gene            complement(2552..3727)
                     /locus_tag="PK614_06360"
     CDS             complement(2552..3727)
                     /locus_tag="PK614_06360"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRB81871.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQEALARGVDIINLGIGD
                     PDLPTPTPIIDSLAQAAKNPKHHQYPSYEGMLSFRKAVADWYKRRFNVALDPADEVLT
                     LIGSKEGIGHVHLAFIDPGDIVLVPSPGYPVYPVGTSFCGGVSHIMPLTKANGFLPDL
                     NAIPKEVAKKAKLMWLNSPNNPTSVIMSKDYFKRVVEFAQENQVIVCHDAAYSEIYYD
                     GKRPASFLEVDGAKDVGVEFHSLSKTYNMTGWRIGFAVGNKGVLAGLGRVKSQLDSGV
                     FEAVQAAGITALGLDDSVTDGLRKIYQERRDTLVPGLKKLGLEVDPPPAAFYIWVTVP
                     KGYTSASFTAHLLEKAGIVTTPGNGFGAPGEGYIRMTVCTTKERLAEAVERIKKAGF"
     gene            3969..5162
                     /locus_tag="PK614_06365"
     CDS             3969..5162
                     /locus_tag="PK614_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRB81872.1"
                     /translation="MNSYGLIQYMDATSLWPAVAALLLAVGTATLLPDIGHLRTTSAA
                     RYSCIDGLRGYLAFAVFLSHSSIWYFYLRSGTWEVPPSNFYTHLGQSSVTLFFMITAF
                     LFWSKLLDGRVQPVDWSRLYLSRVFRLVPLYLLVVIGVVSIALYRAGFRLQESMTSFA
                     LHIASWLTFTIPGIAPVNGFAETIPLAGAVWSLPYEWLFYACLPLGSWCLRAATPTSW
                     LLFSATAVLTLTTVMPDVRGPVLYAFGGGIAAASLARVDAIRTRLAHRVWGLVVVACL
                     GMTMWLFPTAYTFPALILLTSAFTIIACGNSIYGILEWPTSVLLSEMGYSLYLLHSLL
                     LFAAYRLVLGEWASTLSATEHWSIVLGLVPVLILLCFATFRLIEQPAMAAVPRCHAWL
                     VAHLN"
BASE COUNT          993 a         1517 c         1639 g         1205 t
ORIGIN      
        1 ggacagcctg gcggttgagc agagaaagcg gctcgagagt cgcaggatca caaaccgcaa
       61 gttcgatata gccgtcagcg accggccttc tcttccggaa gaagcgaccc gacagcttcg
      121 ggtgcggctg attcaagaag agttcgaaga gctgcaggag gccatggtcg cccaggatct
      181 ccccggggtg gcgaaggagc tggccgatct gttgtatgtg gtctatggca cagccgtgtc
      241 ctatggattg gatatggatc cggtgtttcg tgaggtgcat cggtcgaatt tgagcaaagt
      301 gggtggctac aagcgagccg atggcaagtg ggtaaaaccg ccgacctatt ctccggccca
      361 ggtggaaccg ctgcttgcgg cgcaatcctc cgggagtttg gcggaccgat cgcaagtgga
      421 cacaagaatg caggagtcag agtcgtagca tgaaagagct gtattgtccg gcgtgcggca
      481 cgccctgtgt cagagtgaca tccgggacga atttgatgga gaagacactg aaccgcttgt
      541 ccatctttca agttcgttgt cagctctgca cggctcgctt tcaggcccgc cggccgggta
      601 accggcagac ctcccaagag tttgatcggc gtgagtaccg ccggctgcgg gcgaacttcg
      661 ccgcgtcgct gatcctggac cagccggcag tcgggggggg gtgatcaccg atatctcaat
      721 gggcggctgt acgttgcaag catcctcctc gctcccgcgg ggtacgttcg tgaaactgat
      781 cgtgcatgct cctgccggac agcccgatat caaagtcgac gccgctatga tttgctccat
      841 ccagccgctg tcgatcgggg tgaagttcct ggagtttgaa cctgatgaca agcagcgtat
      901 gggtcagctg gtcctcgaac tcctcgcgca tcaacaagcg cctccccgcg tcagcgcata
      961 acaatcacac ggctcaactt cgtggcattc gcgtgactcc cgcctccttc cggtgtcggg
     1021 tcgcgattgc gcgtgaagct tcgatgttag agcttgcggc ggaatgacgt cagcacattg
     1081 tcaaggaggc gtaccgtccc gatgcggatg gccccgagta ggacagcctg gcggttgagc
     1141 agagaaagcg gctcgagagt cgcaggatca caaactgcaa ggtactccac agttgcgagc
     1201 ggctctgtcg tgaccacgcg cagcatgtgg cgccggatac gcgttccatt ccgttctccg
     1261 gcacgaatcg ccgctacgcc ggcctggagt gcgcgagaga gcacaggcgc cgcctgccgc
     1321 tctgaactcg acagatagac attgcgtgaa ctgagcgcca gcccatcggc ctcccgcatg
     1381 gtcggatgga caatgggaag cccgggcaag ttgagatcct tcacgagttg ttgcaccagg
     1441 gcggcttgct gatagtcttt ttgtccgaac cacgtcttgt cgggccggac cagggacagc
     1501 aacttagtga cgactgtcgc cacgccttga aaatggtgcg gtcgcgcttc cccttcccag
     1561 cgccgggcta tccccgggac agtcacaacc gtttgagcgc cggtcggata catcgtcctg
     1621 acggtggggg caaaaatgac atccacccct tcctttcggc acagtgcttg atcacgtcga
     1681 agctggcgtg gatatttcga aaggtcttcc gtcggcccga attgtgtcgg gttcacaaag
     1741 atgctcacca caacagcgtc gcaggccagt cgggcggcac ggatcaaggc gcggtgccct
     1801 tcgtgtaagg ctcccatcgt cggcacgaac ccgatcgtga tgccttctcg atgaagtcgg
     1861 cggctccagt cggccatggc cggcgtcgcg cgaatgatct tcacgcttgt ggccctgctg
     1921 ttgggggtgc gctgcaggtt ggacgtgatc catagcggga gttcggttgg ggctcgagca
     1981 ggcgaacgat cgatggatca gtgagttgcg ataagagatc gcgcaggttt tgtccgagca
     2041 ccggatgtcg tcgctcggga tcgagttcga ccaacggggt gagcacaaat cgtcgttgat
     2101 gcaggcgggg atgcgggacg gtcagggccg gctcggcgag aatgcagtca tcatacagca
     2161 gtacgtcgag atccagggtg cgaggcccct tgcggttgtc ttgatcgcgc cctagcgccc
     2221 gctcgatctc acggcagatt tccagcagac tcttcggggc gatgttcgtt tcgatctgta
     2281 ccacaccgtt gaggaaccag gaggggccgg gggcggcccc gtcctcgagc ggttcggttt
     2341 cgtagagcga tgacaccgcc tccagccgtg aatgtgggag caggccgagc agtgtcacgg
     2401 cccgatcaca aaagtcgatc cggtttccca ggttagagcc gaaaccgatg aatgctgtgg
     2461 ccatcgtggt cctgacaggg ctcaatggca catgaggata ttcgtatcgg gcccctcttc
     2521 tcagaatgga agaggggccg tcacgcttgc cttagaatcc ggctttcttg attcgttcga
     2581 ctgcttccgc cagtcgttcc ttcgtcgtgc acaccgtcat gcgaatgtag ccttcgcccg
     2641 gggcaccgaa cccgttcccc ggtgtcgtga cgataccggc cttctccagg aggtgggcgg
     2701 tgaaggaggc ggaggtataa cctttgggaa ccgtcaccca gatgtagaag gccgccggag
     2761 gcggatcgac ttccaggccg agcttcttca acccgggcac gagggtatcg cgccgttcct
     2821 gataaatctt tcgcagtcca tcggtgaccg aatcgtccaa cccgagcgcg gtgattcccg
     2881 ctgcttgcac agcttcgaat acgcctgagt ccaactggct tttcacgcgg cccagtccgg
     2941 ccaacacccc tttgttcccg acggcgaatc cgatccgcca gccggtcatg ttgtaggtct
     3001 tggaaagcga gtggaattcc acaccgacat ccttggcccc gtccacttcg agaaaactcg
     3061 ccggacgctt gccgtcgtag taaatctccg agtaggccgc gtcgtgacag acgatcacct
     3121 ggttctcctg ggcaaattcc accacgcgct tgaagtagtc cttgctcatg atcaccgagg
     3181 tgggattgtt cggtgaattc agccacatca gcttcgcttt cttcgcgact tctttcggga
     3241 tggcgttgag gtcgggcagg aagccgttgg cctttgtcag cggcatgatg tgggagacgc
     3301 caccgcaaaa gcttgttccg accggataaa ccggataccc tgggctgggc acgaggacga
     3361 tgtctcccgg atcgataaat gccagatgga cgtggccgat cccttccttc gatccgatca
     3421 aggtcagcac ttcatccgcg ggatcgagcg ccacattgaa gcgccgcttg taccaatccg
     3481 cgacggcctt acgaaacgac agcatgcctt cataagaggg atattggtga tgcttcgggt
     3541 ttttggcggc ttgggccagg ctgtcgatga tcggcgtggg cgtggggaga tcaggatcac
     3601 cgataccgag attgatgata tcgactcccc gcgcaagtgc ctcctgtttc atcttgtcga
     3661 tggcggcgaa caagtaaggc ggcagcgtct taatgcgggt tgcgacttcg atcgggaaac
     3721 cggccatgcg tgaggactcc tttgttgacc tgagcgtcag cgctcagctg tgtgcaatcg
     3781 gtgagcggct gttcgccctc aacctgggat caataaggag ctagggtacc gcaggatcac
     3841 tccggcaatc aacggagtgc tatgggaaga ggcgtgatgc ccggtggtgg ttgggcgagg
     3901 ggctgggagc cggactcgcg tactcgtgag ccatacaccg gtcaagttgc gggaaggctt
     3961 agacaaatgt gaactcctat ggtctcatcc aatacatgga tgctaccagc ctgtggcctg
     4021 ctgtcgcggc gctcttgctt gcagtgggca ctgccacact tcttccggac atcggccatc
     4081 ttcgaaccac atcggcagcc cgatattcgt gtatagatgg actccgggga tatctggcct
     4141 tcgccgtgtt tctcagccat tcttccatct ggtatttcta tctgcgttcc ggcacgtggg
     4201 aggtccctcc gtctaatttt tacacgcact tgggacagag cagcgtgacc ctctttttca
     4261 tgatcaccgc ctttctcttt tggtccaagc tacttgacgg gcgagtgcaa ccggtcgact
     4321 ggtcgcgact gtacctgtcc cgggtatttc gactcgtgcc gttgtatcta ttggtggtga
     4381 tcggtgtggt gagtatcgcg ctctaccgtg ccggatttcg actgcaggaa tcgatgacct
     4441 cctttgcgct gcacatcgcg agctggctca cgtttacgat tcctgggatt gcccctgtca
     4501 atggctttgc cgaaacgatt cccttggccg gggccgtgtg gtcgttgccg tatgaatggt
     4561 tgttctacgc atgccttccc ctcggctcat ggtgtcttcg tgcggcgacg ccgacctcgt
     4621 ggctgctgtt cagcgccacg gcagtcctga cactcacgac ggtgatgccg gacgttcgcg
     4681 gccctgtgtt gtatgcgttc ggtggaggga tcgcggctgc gagcctggcg cgtgtggatg
     4741 ccatacgtac gaggctcgcc catcgagtct ggggtttggt cgtggtcgcc tgtcttggga
     4801 tgactatgtg gctctttccg acggcgtata cgtttcccgc cttgatacta ctgaccagcg
     4861 cgttcacgat tatcgcctgt gggaactcga tctacggcat tcttgaatgg ccgacgtccg
     4921 tcctgttgag cgaaatgggg tacagtctgt acctactgca tagcctgttg ttgtttgccg
     4981 cctatcggtt ggtactcggc gaatgggcgt caactctctc ggccactgaa cattggtcca
     5041 ttgtgctagg cctcgtgccc gttctcattc tgctgtgttt tgccacgttt cgcctgatcg
     5101 aacaaccggc gatggcggcc gtaccccggt gtcatgcctg gttggttgct cacctcaact
     5161 aaagcccata ggcaggagcc tgcaagacct ctggcagggc ggttgacagg ggaggggcct
     5221 ctcattacag ttcgataatg aggtgttcaa cctcgctggg atcttggggg gggccttaat
     5281 cctgtcaacg ttctccggct tggagcacct acccgtaggc tctataaaat gtttataaca
     5341 ctattaaaag atca
//
LOCUS       DAORQN010000097         5304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_633_length_5304_cov_10.364641, whole genome shotgun sequence.
ACCESSION   DAORQN010000097 DAORQN010000000
VERSION     DAORQN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_5304_cov_10.364641"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK614_06370"
     CDS             complement(<1..481)
                     /locus_tag="PK614_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81873.1"
                     /translation="MLVAATIGLMMGWLLRSFATSEKRQQLSEIATRLRGREHELDTL
                     NHELKVRTSAVQMLEGKMITSEAALKDLTADLATKVEQLTTLQTEVREKGVRLHAAER
                     ERDTLRREVEEAEARIKAQSTGFAEMQAQMENAEDVLIARDQEIATLKTWVEQLAPKD
                     "
     gene            complement(519..1424)
                     /locus_tag="PK614_06375"
     CDS             complement(519..1424)
                     /locus_tag="PK614_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB81874.1"
                     /translation="MSRGIIFGVALFALTLLAFLCIPRHLPVTSSATGHSAFSAHIEN
                     GQLTLSGTIASEEAKGAAVARAQDLAKSLRLRVTDNLNIIEDGQAAGWEAALPALLTQ
                     AVTLQHHQATLSLSGQTVTVKGAVPSADAKTKLLHEVASLLGTSIHVQDQVTVASSGS
                     ASQANPAPASQPSAAPHASRAQVQAGLDEILRGEHIAFESNSAILTSKGRAVVDKLVP
                     ALKRAPEAIIEIGGHTDSYGDPDYNLQLSRTRAEAVRQYLVEHQVPNRLTAVGYGSTR
                     PLSQDRTRAASKKNRRIEFRVKEER"
     gene            1777..3330
                     /gene="purH"
                     /locus_tag="PK614_06380"
     CDS             1777..3330
                     /gene="purH"
                     /locus_tag="PK614_06380"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRB81875.1"
                     /translation="MASIARALISVSEKTGVVEMAKGLAALGAEVLSTGGTAKALREA
                     GVAVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRRVPDHVKQMQQHGIGNIDVVVV
                     NLYPFESTIAKPNCPFEEAIENIDIGGPSMLRSAAKNHEDVLVLVDPADYGRVLEALK
                     SGSVTPALRRELAMKVFQHTARYDSLIAGYLEKQVQGSEVKFPAILSLQFERVETLRY
                     GENPHQQGAFYREMNAKEPAVSRGKILHGKAMSYNNFLDSNSALELVKEFEQTAVAII
                     KHNNPCGCALGATPVDAYVKARATDPVSAFGGVIAFNRPVDLAAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDIRLLDVGPLSKATAEGYDLKKLVGGLIVQDRDLGTIKDIKAL
                     AVPTSRKPTEEEYAACAFAWVVCKHVKSNAIIYGRPGEIVGIGAGQMSRVDSVKLAVM
                     KAQSPVKGCVMASDAFFPFRDGIDAAAEAGITCVIQPGGSIRDPEVTKAVDEHGMAMI
                     LTGMRHFRH"
     gene            3576..4853
                     /gene="purD"
                     /locus_tag="PK614_06385"
     CDS             3576..4853
                     /gene="purD"
                     /locus_tag="PK614_06385"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRB81876.1"
                     /translation="MKILVVGGGGREHAMVWKIAQSPRKPQIFCAPGNAGIEGLATCV
                     PIKADDIEGLKAFALKEQIDLTVVGPEAPLALGIADEFRKARLKIFGPTKAAARLESS
                     KSFSKDIMAANRIPTAAARSFEHMDQALAYLDEQPVPIVVKADGLAQGKGVVVATTRE
                     EAKQAVRDAMEKSLFGQAGHRVLIEEFLDGEELTLMAFTDGKTVVPMVPAQDHKRVGD
                     GDSGPNTGGMGAYAPAPIATAALREQVTRQVLQPTVDALARLGCPFQGVLYAGLMVVK
                     GTPYVLEFNARMGDPETEVVLPLLKTDLVEVMEAVVEHRLDELTVEWHPDTAVCVVMT
                     SPGYPGSYPTGLPIQGLPTPFGESRVAVFHAGTKRESGRVVTAGGRVLAVTAWGPSLL
                     DARAQVYQAVPSILFEGRHYRTDIAHRALPHKI"
     gene            4880..>5304
                     /locus_tag="PK614_06390"
     CDS             4880..>5304
                     /locus_tag="PK614_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRB81877.1"
                     /translation="MALILPFTDTTCDRVLPDARQVLDAHGVLALPTETYYGLAVRPT
                     DETALRRLIELKGRPSDKPILVLIGSLDQLPQLVQAIPPAAALLMEQFWPGPLTIVFP
                     AAPDLPALLTAGTGTIGVRQAPLLQLQHLLRHTGPLTGT"
BASE COUNT          952 a         1516 c         1701 g         1135 t
ORIGIN      
        1 catctttcgg tgccagttgc tccacccagg tttttaaggt agcaatctcc tggtcgcggg
       61 caatcagcac gtcttcggca ttctccatct gcgcctgcat ttcggcaaat cccgtcgatt
      121 gtgccttgat cctggcttcg gcttcctcga cctcgcgtcg gagagtatcc cgttcacgtt
      181 cggcggcatg caggcgcaca cccttttccc ggacttccgt ctgcaaggtg gtgagttgtt
      241 cgaccttggt ggcgaggtcg gcggtcaggt ctttgagcgc cgcttccgag gtgatcatct
      301 tgccttccag catctggacg gcagaggtgc gcactttcaa ctcatggttg agggtatcca
      361 gttcatgttc gcgcccgcgg agtcgggtgg cgatttcgga cagctgctga cgtttttcag
      421 aagtggcaaa gctgcggagc agccagccca tcatcagtcc gatggttgcc gccaccagca
      481 agcaccccac catttgcaga atcatggctc ccataggttc accgttcctc tttcactcga
      541 aattcgatgc gccggttttt cttcgacgcg gcgcgcgtcc ggtcctgact cagggggcgc
      601 gtcgatccat aaccgacggc ggtcagccga ttcggtacct gatgttccac gaggtattgc
      661 cggactgcct cggctctggt gcggctcagt tggaggttgt agtcgggatc gccgtaggag
      721 tcggtgtggc cgccgatttc gatgatcgcc tcgggtgccc gcttaagcgc cggcaccagt
      781 ttgtcgacca cggctcgtcc cttcgaagtc aggatcgcgc tgttactttc gaatgcgata
      841 tgttcgcccc gcaatatctc atccagcccg gcttggactt gggctcggga ggcatgcggt
      901 gcggcggacg gctggctggc gggagcaggg ttggcctgac tcgcggagcc tgacgacgcg
      961 acggtcacct ggtcttgcac gtgtatagag gttccgagca gtgaagccac ttcatggagc
     1021 agttttgttt ttgcgtccgc gcttgggact gcgcctttta ccgtcaccgt ctggccggag
     1081 agggagaggg tggcctgatg gtgctggagt gtgaccgcct gtgtgaggag cgcggggagg
     1141 gcggcttccc atccagcggc ctggccgtct tcgatgatgt tcagattgtc ggtgacacgc
     1201 agcctgaggc tcttggcgag atcctgtgcg cgcgccaccg cggcgccttt ggcctcctcg
     1261 ctggctatcg tgccggacag tgtcagctgg ccgttttcaa tgtgggcact gaacgcggag
     1321 tgaccggtcg ccgacgacgt caccggcagg tgtcgaggga tacagaggaa cgccaataat
     1381 gtgagggcga agagtgctac gccgaaaata attccgcgcg acatgcgcaa gcctttatcg
     1441 tctcaggcac gaggccccgc tgcggcatcg agtcgccgtg ggctggtgtc gtttcaagac
     1501 ttcgagtgtt acttgattaa gctgatgcgt cacctcatgt tggcgcatct ctatcggcgg
     1561 aagggtatca catcgccgat actcatgcta gcctggattc ttcagcagag gccggcgtct
     1621 gccgggtgat ttcgcgtcgc gcagccgtgc cgttcggggt cgagttcagg tgtgtccggc
     1681 ttgcagtggc cgtgagccat ctggtattgt cacggcgctt ttcgtctagc tcatggtttc
     1741 ccacccacac gatatcaata gttgaaggag ttcatcatgg ccagcattgc gcgggcactg
     1801 atcagtgttt ctgagaagac cggagtcgtc gagatggcga aggggctggc ggccctaggc
     1861 gccgaagtgt tgtccaccgg tggaaccgcg aaggccttgc gtgaggcagg cgtggctgtc
     1921 accgacgtgg ccgcctatac ggggtcgccg gagatcctgg acgggcgcgt gaaaacgttg
     1981 cacccgaaaa ttcatggcgg actcctcggc cgccgtcgcg tgcccgatca tgtgaagcag
     2041 atgcagcaac atggcatcgg caacatcgat gtggtggtgg tcaatctcta tccctttgaa
     2101 tcgacgatcg cgaagcccaa ttgtccgttc gaagaggcca ttgaaaatat cgacatcggc
     2161 ggtccgtcga tgttgcgatc cgccgcgaag aatcatgaag acgtgctggt cctggtcgat
     2221 cccgccgact acggccgggt gctggaggcg ctgaagtccg gttccgtgac ccccgcgttg
     2281 cgccgcgagt tggccatgaa ggtgtttcag catacggccc gctacgacag cctgatcgcc
     2341 gggtatcttg aaaagcaggt gcaggggagc gaggtgaagt tccccgccat cctctcgttg
     2401 cagtttgagc gtgtggagac gctgcgctac ggagaaaatc cccatcagca gggggcgttc
     2461 tatcgtgaaa tgaacgccaa ggaaccggcc gtatcgcgcg ggaaaattct gcacggcaag
     2521 gccatgtcct acaataattt cctcgactcc aactcggcgc tcgaactcgt gaaggagttc
     2581 gagcagaccg cggtggctat catcaagcac aataacccct gcggctgcgc actcggtgcg
     2641 acaccggtgg acgcctatgt gaaggcgcgc gcgaccgacc ccgtgtcggc gttcggcggt
     2701 gtcattgcct tcaatcgtcc ggtggatttg gctgcggcca aggaaatcac ctcaactttc
     2761 gtcgaggtcg tcatcgcgcc gggcttcgcc gaggacgcgc tggctgaact caagcgcaag
     2821 aaggacatcc gtctcctgga cgtgggaccg ctgagcaagg cgactgcgga ggggtacgat
     2881 ctcaagaagc tggtcggcgg attgatcgta caggatcgcg atctgggcac gatcaaggac
     2941 atcaaggcat tggcggttcc gacctcgcgc aagccgaccg aggaagaata tgccgcctgc
     3001 gcgtttgcct gggtggtctg taaacatgtg aagtcgaacg ccatcatcta cgggcgtccg
     3061 ggcgaaatcg tcggaatcgg agccgggcag atgagccggg tggactcggt gaaactggcc
     3121 gtcatgaaag cccagtcgcc ggtgaagggc tgcgtgatgg catccgacgc atttttcccg
     3181 ttccgtgacg gtatcgacgc ggcggcggaa gccggcatta cctgtgtgat tcagccgggt
     3241 ggttcgatcc gcgaccctga ggtgacgaag gccgtggacg agcatgggat ggcgatgata
     3301 ctcacgggca tgcgccactt ccgccattga gggacgttga aacgggcctc caacttcgtt
     3361 ctcggcgaga aaaaatcctc aacgtagccc tgaggctacg ccttcggttt tttcttgcct
     3421 gcggccttgt tggccatccc gtttgaacgc cctcagggga tgtgtcttct ttcgcttatc
     3481 tggacgagca ggaaaacgtc tcgacatcgg caattctgac gagcctgcca cttacaaaac
     3541 gctgcttcgt ttttatttaa agtaaaggtt gagttgtgaa gattcttgtt gtcggtggcg
     3601 gtgggcggga acatgccatg gtgtggaaga tcgcccaaag tcctcggaag ccgcagatat
     3661 tctgtgcgcc ggggaatgcc ggcatcgaag gtctcgccac ctgtgtcccg atcaaggccg
     3721 atgatatcga agggctcaag gcgtttgcgc tcaaggagca gatcgatctc actgtagtcg
     3781 gcccggaagc gccgttggcg ttgggcatcg cggatgagtt tcgcaaggcg cgactaaaaa
     3841 tcttcggacc gaccaaggcc gccgctcgcc tggaatccag caagagcttc tccaaagaca
     3901 tcatggccgc caatcgaatc ccgaccgcgg ccgcgcgcag cttcgaacac atggaccagg
     3961 cgctggccta tctggacgag cagccggttc cgattgtggt caaggccgac ggtctcgcgc
     4021 agggcaaagg ggtggtcgtg gctacgacac gcgaggaagc caagcaggcc gtccgcgatg
     4081 ccatggaaaa atccctgttc ggtcaggccg gacatcgcgt gctgatcgaa gagtttctgg
     4141 acggcgaaga gttaacgctc atggccttca ccgacgggaa aaccgtggtc cccatggtgc
     4201 cggctcagga ccacaaacga gtgggagacg gcgacagcgg acccaatacc ggcggcatgg
     4261 gcgcctatgc tccggcgccg atcgccacgg ctgcgctgcg tgaacaggtg acgcgtcagg
     4321 tgctgcagcc gacggtggat gcgctggccc gtctgggttg cccctttcaa ggtgtgctct
     4381 acgcgggcct catggtcgtg aaggggaccc cctatgtgct ggagttcaac gcgcgtatgg
     4441 gagatcctga aactgaagtc gtgctgccgc tcttgaaaac cgatctggtc gaggtgatgg
     4501 aggcggtggt cgagcaccgt ctggatgaat tgacggtgga atggcatccg gatacggccg
     4561 tgtgcgtcgt gatgacgtcg ccgggttatc ccggttccta tccgaccggc cttcccattc
     4621 aggggctgcc tactccattc ggtgaatcgc gcgtcgcggt gttccacgcc ggtacgaaac
     4681 gggagtcggg gcgcgtggtg actgcgggag gacgagttct ggcggtcacc gcctgggggc
     4741 cgtctttgct cgatgcgcgg gcgcaggtgt atcaagcggt gccttcgatt ttgtttgaag
     4801 ggcgccatta tcgaaccgat atcgcgcatc gggccttgcc gcacaagatc tgatccgagc
     4861 agttttcggc cattattcca tggctctgat ccttcccttt accgacacaa cctgcgatcg
     4921 cgttctgcct gatgcccgac aggtgttgga tgcgcatggc gttctcgcgc tgcccaccga
     4981 aacgtactac ggtttggcgg tccgtccgac ggatgagacg gcgctccggc gtctcatcga
     5041 gttgaaaggg cggccatccg ataagccgat tctggtgctt atcggaagcc tggaccaatt
     5101 gcctcagttg gtccaggcga ttcctcccgc cgcagccctc ctgatggaac agttctggcc
     5161 cgggcctctg accatcgtgt ttcctgcggc gcctgacttg ccggcgctgt tgacggcggg
     5221 gaccggaacg atcggggtgc ggcaggctcc cttgcttcag ctccagcatt tgttgcggca
     5281 caccggcccc ctgaccggga ccag
//
LOCUS       DAORQN010000098         5289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_634_length_5289_cov_9.295376, whole genome shotgun sequence.
ACCESSION   DAORQN010000098 DAORQN010000000
VERSION     DAORQN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_5289_cov_9.295376"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PK614_06395"
     CDS             complement(<1..984)
                     /locus_tag="PK614_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRB81878.1"
                     /translation="MILAVLAVFLASCAAPKSPTGGPVDTLPPVIIAEESTPNQQTNF
                     REDEITITFDEWFTIKDVATQLVISPLMPEQPVVKQKGKSIVITLPDSLLEETTYTLN
                     FGSAIADLNEGNVLENYAFVFSTGDVLDSVKMSGTVINAVTLTPAEGVWVMLYPVGID
                     SAVYKRKPEYVAKTNKEGKWAMSNIRADSFNVVALKDDNLNFLYDQDTELFGWIDYNI
                     YTLRPTALLPEIKVFPKVRQPIIRDVIHTVPGWIKVIVDGPVPKQTPRFNPEVEGAVT
                     AWDGDTLHVWYNAVKNYFGEAILLEDTTRIKIAPGASPISWPLRVTTVTSRL"
     gene            1101..1856
                     /locus_tag="PK614_06400"
     CDS             1101..1856
                     /locus_tag="PK614_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRB81879.1"
                     /translation="MKRYCLDTDSPAFQYPIANKKGKVKRYCLETDCYFLSIPNSQQE
                     RKSVRFLSGDRFSCLSIPNSQQERKSGKILSGDRFSCFSISNSQQGRKSEKEKNCSCF
                     FGQQGILLSLHPLWILKSPVKNKPLMARNNLEERLIDFAVLIIQIVNKMPNTVAGKQL
                     SSQLIRSGTSPALNYGEAQGAESRKDFVHKLKVVLKELRESHINLRIIHKGNILVNDE
                     HVIYGIQESDELVAIFIKSIETAKRNLAKEGKT"
     gene            complement(2040..2966)
                     /gene="xerD"
                     /locus_tag="PK614_06405"
     CDS             complement(2040..2966)
                     /gene="xerD"
                     /locus_tag="PK614_06405"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRB81880.1"
                     /translation="MRQQMDWDSTIKGFIAYLTLEKGLSGHSIEAYTRDVRRIALFMV
                     KDLGVNSPTVVTSHQIEQFLHVLNEVEISHRTQARMLSGIRAFYQYLILEDMLAADPT
                     ELVDSPKTGQYLPEVLSYEEIRRMIDSIDQSHPQGVRNVAIIETLYACGLRVSELTGL
                     LISNIFPDMQIIRVIGKNNKERLVPIGQEALHQIKMYLEGDRNRMMNIDSTSKDILFL
                     NRRGKKLSRVMIFNIIKDTAANAGVTKTISPHSLRHSFATHLVEGGADLRAVQEMLGH
                     ASITTTEIYTHLNADYLRETINAFHPLNRGFE"
     gene            complement(3009..>5289)
                     /locus_tag="PK614_06410"
     CDS             complement(3009..>5289)
                     /locus_tag="PK614_06410"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRB81881.1"
                     /translation="EFTDVDGTFQFVEIPAGSYYLNVSFLGYKDYSTETFEVVDGETV
                     SPLPEIILRPDGLELAEVSVVAQRPFIERRADRMIVNVENSILAAGSNALEVLERAPG
                     IVVSAGESISIRGRSGVIFMIDGKITPMSGQELANYLKAMPAGSIDRIEIITNPSAKY
                     DAAGNAGIIDIRLKKDANMGTNGTLTSSYSQGVYPKAGIGVSLNRRSKDLNIFGGYNY
                     NYREGFNDLRLYRAFFENGQRTGAYDQKNYLVIPYHFHSARFGVDYTISPNTLVGILA
                     NGNINAFKPSGQNKSNVENGAQEVVSAFATTNESSDLWPSYSLNANLKHTFPKKKQEL
                     TVDLDVARYWNETEQQFTTRYYDVNGEEYLPFYLLVGDLNGNLNIKSAKADYAYPINE
                     AARFEVGVKTSLVSADNDLQFYDESDVNNPVFDSTISNHFLYDELINAAYVNFNYNWT
                     KFSIQSGLRVEQTVADGTQLINGVSFDRNYTNLFPSVFLNYSFSDNYAMGLNMSRRLD
                     RPSYQQLNPFKFFLDPSTYREGNPYLNPQFTWSFEWNHTLFQRYTASLSYARTTDNIT
                     QVVGPVEGEERVTVQTDRNLDVADYYSLSVGIPVNIGKWWSSNNSFSCYLGNYLGNFA
                     DTELNDGNLVLDMRTNNTFILGNDWAAELNFSYHTREQYAFMNLDPMWGLGAGIQKQL
                     FKKRSTLKLTFTDIFWTNLPSAYIKYTNYEESFDVYRDTRMATVSYTHRFGDSQVAPS
                     RRRAGGAEDEKQRAAAVQG"
BASE COUNT         1448 a         1151 c         1224 g         1466 t
ORIGIN      
        1 caatctgctc gtcactgtcg tcacgcgcag aggccaggat atcggtgaag ctccaggagc
       61 gattttaata cgtgtagtat cctcaagcaa aatagcttct ccaaaataat ttttcactgc
      121 attgtaccag acatgcagtg tatcaccatc ccatgcagtg actgctcctt caacttccgg
      181 attaaaccga ggcgtctgct taggtaccgg accgtcaaca attactttaa tccatccagg
      241 caccgtatgt attacatcac ggatgatcgg ttgccttact ttgggaaaaa ccttgatctc
      301 cggaagtaat gcagttggtc tcagggtata aatattgtaa tcgatccatc caaacaattc
      361 ggtgtcctga tcatacagaa aattgagatt gtcatccttg agtgccacga cattaaatga
      421 atcagcgcgt atgttcgaca tcgcccattt accttctttg ttggtcttgg ctacgtattc
      481 aggcttgcgc ttgtacacag cactatctat tcctaccgga tacagcatga cccacacgcc
      541 ttctgcagga gtgagtgtaa ccgcattgat gacagtgcca ctcatcttga cagaatcgag
      601 tacatcacca gttgagaata cgaaagcata attctccagc acattgcctt cattgagatc
      661 agcaatcgcg ctaccgaagt taagtgtgta ggttgtttct tccagcagac tatccggcag
      721 cgtgatgacg attgattttc ctttttgttt gaccaccggc tgttccggca tgagtggaga
      781 tatcaccagt tgtgtggcga catccttgat cgtaaaccac tcatcaaatg tgatggtgat
      841 ttcatcttct ctaaaattgg tttgttggtt gggagtcgat tcttccgcaa taatgaccgg
      901 aggcagcgta tccacaggac cgccggtcgg gctctttgga gccgcgcacg aagccaggaa
      961 gaccgccagg acggccaata tcatggaaaa agggtgcttt tggaaaaaca tggggcaaat
     1021 atatcagtat tcaaaggaac gtctgtcagg ggacagaaag tactgagtat caatacccaa
     1081 tagccaacaa ggaaggaaaa gtgaaaaggt attgtctgga tacagattct cctgcttttc
     1141 aatatccaat agccaacaag aaaggaaaag tgaaaaggta ttgtctggag acagattgtt
     1201 actttttatc aatacccaat agccagcaag aaaggaaaag tgtgaggttc ttgtcgggag
     1261 acagattttc ctgtttgtca atacccaata gccagcaaga aaggaaaagt ggaaagattt
     1321 tgtcgggaga cagattctcc tgcttttcaa tatccaatag ccaacaagga aggaaaagtg
     1381 aaaaggagaa aaattgttct tgcttttttg gtcagcaggg aatattattg tctttacatc
     1441 cattatggat tttgaaatca ccagttaaaa acaaaccact catggcacga aataaccttg
     1501 aggaacgact cattgatttt gcagtcttga tcattcaaat cgtcaataaa atgccaaaca
     1561 cggttgcagg gaaacaactt tcttctcagc tcatacgatc aggcacttct ccggcattga
     1621 attatggcga agctcaaggc gcagaatcac gcaaagattt tgtccacaaa cttaaggttg
     1681 tactcaaaga attgagggaa tctcatatta accttcggat tatccataaa ggaaacatcc
     1741 tcgtaaatga tgaacacgtc atttatggca tacaggaaag tgatgaactt gttgccatct
     1801 ttatcaaaag catagaaact gctaaaagaa atttagcaaa ggaaggcaaa acataaatct
     1861 gtatccagac aaatacttct cacttttcat tggctactcg tgctgagcgt agtcgtagta
     1921 ttggctattc catattgaaa aaagccaaga actgtctcct gacaaatact tcccactttt
     1981 ccttggctat tggctattcc ttattgagaa aaagcataat ctgccttcag gcagaaactc
     2041 tattcaaatc ctctgttcaa cggatgaaac gcattgatcg tctcacgcag ataatcagca
     2101 ttgagatgtg tgtagatttc agtggtggtg atagacgcat ggccaagcat ttcttgcacg
     2161 gcgcgaagat cggcgccgcc ttcgacgaga tgagtggcga aggaatgacg aaggctgtgg
     2221 ggtgatatcg tcttcgtgac accggcattg gctgcggtgt ctttgatgat gttgaagatc
     2281 atgacgcgcg ataatttctt tccgcggcgg ttgagaaaca ggatgtcttt cgaggtgctg
     2341 tcgatattca tcatgcggtt acggtcgcct tcgaggtaca tcttgatttg atgaagtgct
     2401 tcctggccga tgggcacgag gcgctctttg ttattctttc cgatgacgcg gatgatctgc
     2461 atgtcgggaa agatattact gatgagtaat cctgtgagtt ccgatacacg aaggccgcat
     2521 gcatacagtg tctcgatgat cgcaacattg cgcacacctt gtggatgact ttggtcaata
     2581 ctgtcgatca tgcgacggat ttcttcataa ctgaggactt caggcagata ctgccctgtc
     2641 ttcggactat cgacgagctc tgtcggatcg gcagcgagca tgtcttcgag aatcagatat
     2701 tgatagaaag cacggatgcc ggacagcatc ctggcctggg ttctatgact gatctctacc
     2761 tcattgagca catggagaaa ttgttcgatc tggtgggatg taacgacagt cggtgaattg
     2821 acgccaagat ctttgaccat aaacaacgcg atgcggcgca catcccgtgt atatgcttca
     2881 atgctatgac cggacagccc cttctcaagc gtgaggtagg caataaaccc ttttatcgtt
     2941 gaatcccaat ccatttgttg gcgcaagtta cagccatcaa atggaaagta ccctcaaccc
     3001 ctatcctgct agccttgaac tgcagcagcg cgctgttttt catcttcagc tcctcctgcc
     3061 ctcctgcggg aaggagctac ctgagaatca ccaaaacggt gcgtatacga taccgttgcc
     3121 atgcgggtat ctctgtagac atcaaatgac tcctcgtagt tagtgtactt gatataagcc
     3181 gaaggtagat tcgtccagaa gatatccgta aaagtcagtt taagtgttga acgtttttta
     3241 aacaattgct tttgaatacc ggcacctaat ccccacattg gatcaagatt cataaaggca
     3301 tattgctcgc gggtatggta agagaaattc aattcagctg cccaatcatt gccgaggata
     3361 aatgtgttgt tggttcgcat gtccaataca agatttccat cattgagttc tgtgtcagca
     3421 aagttcccga ggtagttgcc gaggtaacaa gagaaggaat tattgctgct ccaccattta
     3481 ccgatattga ctggtatgcc tacagaaagt gagtaataat ccgcaacatc aagatttcga
     3541 tcagtttgca cggtgacgcg ttcttctcct tctacgggcc caactacttg tgtgatattg
     3601 tcagtggttc gtgcatatga aagagaagct gtatagcgtt gaaacaaggt atggttccac
     3661 tcaaatgacc aggtgaactg cggattgaga taaggattcc cttcacgata cgtgcttgga
     3721 tcaaggaaaa acttaaacgg attcaattgc tgatatgaag gtcggtcaag gcggcggctc
     3781 atgttaagac ccatcgcgta gttatctgaa aatgaatagt tgagaaacac gcttggaaac
     3841 agatttgtgt aattgcggtc aaaggaaaca ccgttgatga gttgagtgcc atctgctact
     3901 gtttgttcga cgcgcagacc agactggatg ctgaatttcg tccagttgta attgaaattg
     3961 acatatgctg cattgatcag ctcatcatac aggaagtgat tactaatggt gctatcgaaa
     4021 accggattgt tgacatcgct ttcgtcatag aattggagat cattatcagc gctgacaaga
     4081 cttgtcttca cccctacctc aaatctggca gcttcattga taggatatgc atagtcagcc
     4141 ttggctgatt tgatgttgag attaccattt agatcaccga cgagcagata aaatggcaaa
     4201 tactcttctc cgttgacatc gtaatagcgg gtagtaaatt gctgttctgt ttcattccag
     4261 tacctggcta catccagatc gacggtgagt tcctgcttct ttttgggaaa ggtgtgtttc
     4321 aggttggcat tcagtgaata cgaaggccat agatcactgc tttcattggt agtggcaaat
     4381 gcggagacca cctcctgagc accgttttct acattgcttt tgttttgccc tgatggttta
     4441 aaagcattga tgtttccatt ggcgagaatt cctaccaggg tattcggtga gatggtgtag
     4501 tcgacaccaa atctcgctga atgaaaatga tatggtatta cgagataatt tttctgatcg
     4561 taggcaccag tgcgttggcc attctcaaaa aaggcgcgat agaggcggag atcattgaag
     4621 ccttcacggt agttgtaatt gtagccaccg aaaatattca ggtctttcga acggcggttg
     4681 agtgaaacac caatacctgc cttgggatac acgccctggc tatagctgga ggtcagggta
     4741 ccattcgttc ccatgttagc atctttcttg agacggatgt ctatgatacc ggcattgcct
     4801 gctgcatcat atttagcgga tggattggta attatctcta tgcgatcgat actaccagct
     4861 ggcatggcct tgaggtaatt ggcgagctct tgtcctgaca tgggcgttat ctttccatca
     4921 atcataaaga tcacgcctga tctgccgcgt atggaaatgg attccccagc gctcacgacg
     4981 atgccaggcg ctctttcgag aacttctaaa gcattggaac ctgctgccag gatactgttt
     5041 tcgacattga cgatcatgcg gtcggctctt ctttcaataa aaggtctttg ggcgacaaca
     5101 ctgacttccg cgagttcgag tccatcagga cggagtataa tttctggaag tggtgaaacg
     5161 gtctcaccat ccactacttc aaatgtttct gttgaataat ccttgtatcc caggaaagag
     5221 acattgagat aataacttcc agccggtatt tctacaaact gaaatgtgcc atcgacgtcg
     5281 gtaaactct
//
LOCUS       DAORQN010000099         5254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_638_length_5254_cov_7.419504, whole genome shotgun sequence.
ACCESSION   DAORQN010000099 DAORQN010000000
VERSION     DAORQN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_5254_cov_7.419504"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1057
                     /locus_tag="PK614_06415"
     CDS             422..1057
                     /locus_tag="PK614_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRB81882.1"
                     /translation="MLTAIGDVMRRVYERGWITTRDGNISMRKRAGKYLYITPSGWRK
                     TIVHPEHIVRLEVLTDPVTGQLVPKVRDGQQPSGELWMHWNLQRDTKKTRTVVHVHAT
                     HIVAAIYAGIDLQAMSAEFPEISRYTRVGHTVPALPALSRDLADVTAECLGLKKDGTL
                     EFDIVGQMNHGVCAVATDPWAAYEHIERLDHICEIVLKSGVASKPVGKQSS"
     gene            complement(1130..1205)
                     /locus_tag="PK614_06420"
     tRNA            complement(1130..1205)
                     /locus_tag="PK614_06420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1170..1172),aa:Asn,seq:gtt)
     gene            complement(1270..1539)
                     /locus_tag="PK614_06425"
     CDS             complement(1270..1539)
                     /locus_tag="PK614_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81883.1"
                     /translation="MEQEDNVLDELLRDIAGLLHEYPKAIERQAAAIHASGKDPELAE
                     KLVKAADTMRDSGNLYLTWAKHFAALAEGNTDASSDEDETDDFGI"
     gene            complement(1619..1897)
                     /locus_tag="PK614_06430"
     CDS             complement(1619..1897)
                     /locus_tag="PK614_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRB81884.1"
                     /translation="MYNVAQVIDEKCVAKKGCRLCIMYCPEANCLDLNVTKMVAEVTI
                     DRCKGCELCVVVCNAAKHEAIEMQAVSATGQLMSHKGESAGLGQAYQG"
     gene            complement(1921..2628)
                     /locus_tag="PK614_06435"
     CDS             complement(1921..2628)
                     /locus_tag="PK614_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRB81885.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVMAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIEVFHPQVITMGKSYTMPFYSGVKEGGVV
                     IINSAQPLLSEEDINRLKDLNVALFYIAGTELAIEVAGTELSTNMAMIGSVAGITKCV
                     SMEALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKAAYEIASEW
                     ADKNKIELRVGNPAVAA"
     gene            complement(2646..3548)
                     /locus_tag="PK614_06440"
     CDS             complement(2646..3548)
                     /locus_tag="PK614_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB81886.1"
                     /translation="MSKERIKISPDLYDIMPSDYQDLVQSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPDRVKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTPICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     IRNSVLIAREIGPTYIQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYVSEPAK
                     QFLAELAAKDKERKAAAKQLAGKA"
     gene            complement(3613..4824)
                     /locus_tag="PK614_06445"
     CDS             complement(3613..4824)
                     /locus_tag="PK614_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB81887.1"
                     /translation="MAEIKSHIGTQNKKGQTFTDAWKMMQEAPRTPSFYTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTIEISYLLNTGML
                     VWHAETAQDFFDWILKGFMVSEEPEVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEIWAAVERSRKHTIKWI
                     NGLIDTENTDAEVVIVASGTAVSQGREAIRLLEDEGMRCGLVKVKSLRPWPGEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIKATIPNSERVHAGPHVCGGMTMPPEIIVSEIKTALG
                     LRSESLAGRGS"
     gene            complement(4867..>5254)
                     /locus_tag="PK614_06450"
     CDS             complement(4867..>5254)
                     /locus_tag="PK614_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HRB81888.1"
                     /translation="YEILKECVQPGQKPMLIPMPDYRPKYQKINPEVEINPNHPNLTI
                     WHNKIDCCMFIGVHCHQANLSLKIIRGGTSCYTIAMCAQAGHEDAMLSFRDASVEKIL
                     KLGEWIRKIKGTVKPRLTSAKTSASN"
BASE COUNT         1112 a         1412 c         1440 g         1290 t
ORIGIN      
        1 gacgttaaat cccgcacggt aggctttggt cgtcatcccg cgcatgtagt gggagtctgc
       61 ggagccctca agcccgtgga gcaggatgag ggtcggggaa ctccgggggt gagactgcca
      121 gtgacagaag ccctgcagct gggttctcgg ttcggtggtg aatcgacgtg tcacctgagg
      181 gaggggtgct cctatcgcct cccgagacca gtagcgaggt atcaaagtca tgagatgcgg
      241 attacggagc aggagtgcgg gaatgaatcc atctagctca gtcatatccg gctcatcata
      301 aacaaatgcc gctccctgcg cacttttcat tttctgtcgg gccggtgcct tctcccctca
      361 agggttgcgg gtgaggaacc gataacagaa gttggaaagt tggttcccca gggaggcagg
      421 tatgctgact gcaatcggtg atgtgatgag gcgagtgtat gaacgaggtt ggattacgac
      481 ccgagacggt aacatcagca tgcggaaacg ggcgggcaag tacctgtata tcacaccgtc
      541 cggatggcga aagacgattg tccatccaga acacatcgtg cggttggagg ttctgactga
      601 tcctgtgacc ggccaacttg tgccaaaggt gcgtgatggg caacagccat ccggggagct
      661 gtggatgcac tggaacctgc agagggatac gaagaagacg aggacggtcg ttcacgtcca
      721 tgcgacccac attgtggctg cgatctatgc cggaatcgat ttgcaggcga tgagtgcgga
      781 gtttcccgag atctcacgtt atacccgtgt cggccacacg gtccctgcat tgccggctct
      841 gtcacgtgat cttgccgatg tgacggcaga gtgcttgggg ctgaagaagg atgggacact
      901 ggagtttgac attgtcggac agatgaatca cggtgtgtgc gctgtcgcga cggacccttg
      961 ggccgcctat gaacatatcg agcgactcga ccatatctgt gagatcgttt tgaagagtgg
     1021 agttgcttcg aagcctgtgg gtaaacagtc gtcttagtga agaggtaaga aggatttggg
     1081 aaaaagagaa gggggctgac ggacaacacc gtcagcaccc ttcgtattgt ggctccccgg
     1141 gcaggactcg aacctgcgac cagccggtta acagccggct gctctaccaa ctgagctacc
     1201 ggggaacaat gctacgcaaa acaaacttcg gcattctagc aaaactgggg agggaagggg
     1261 gagacttttt tagatgccga aatcgtccgt ctcgtcctca tccgaagagg cgtccgtatt
     1321 gccttccgcc agtgcagcaa aatgttttgc ccatgtgaga tagaggtttc cgctgtcccg
     1381 catcgtatca gcggctttga ccagcttttc tgccagttca ggatcttttc cactcgcgtg
     1441 tatggctgca gcttgccgtt cgatggcttt gggatattcg tgcagtaggc cggcaatatc
     1501 gcggaggagt tcatcgagca cattgtcttc ttgttccatg gaacctcctc gagcgctcat
     1561 cccaagaaaa aaccccatag tgtgggacgc tatggggttt atgagacgaa gtgcctgctt
     1621 atccttggta ggcttgtccg aggcctgcgg actcgccctt gtggctcatc agctgcccag
     1681 tggcgctgac cgcctgcatc tcgatcgcct cgtgcttggc ggcgttacag acgacgacgc
     1741 acagctcaca tcccttgcag cgatcaatcg tgacttctgc caccatcttg gtgacgttga
     1801 ggtccaagca gttagcttcc ggacaataca tgatgcacag gcggcagccc ttcttggcca
     1861 cgcacttctc atcgatgact tgcgctacgt tatacatgcg acgtggttcc ttctctctcg
     1921 ttaagccgca acggcaggat tgccgacccg caactcgatc ttattcttgt cggcccattc
     1981 gctcgcgatc tcatatgccg ctttgaccgt cgccaggttc ttcgccaaca acatttcctt
     2041 cttggcgaat ttctttttga tcgcctcgtc caaggacgca gttccgcctg atgcgacaaa
     2101 tttctttccg aagcgctctt gcaaggcacc atcgagagct tccatggaga cgcacttggt
     2161 aatgcctgcg accgagccaa tcatggccat gtttgttgag agctctgtgc cagccacttc
     2221 gatcgcgagt tctgttcctg caatgtaaaa caaggccacg ttcagatcct tgagtcggtt
     2281 gatgtcctcc tccgacaaaa ggggttgcgc tgagttgatg atgacgacgc ccccttcttt
     2341 gacaccggag tagaaaggca tggtatagct ctttcccatc gtaatgacct ggggatgaaa
     2401 cacttcgatc acatcgggaa ataccagctc tcctcggtca tagattcgct caatcccgat
     2461 ccggcagtag ctttctgctg gggccatgcg cttttcagcg ccaaaaaatg gattcgaaat
     2521 ggaaaacttt ccatcccggt tggcggccat agccattaca tgggctgccg tcacggcacc
     2581 ttgtcctccc aacccggaca tgcggatatt gagcctcttc ttgatcatga gcgacctccg
     2641 gttccttaag cttttccagc taactgtttg gcggcggctt tgcgttcctt atccttcgcg
     2701 gctagctcgg ccaggaactg cttcgccggc tcgctgacat actctttata agcaaatcgc
     2761 tcagtcggct tttcagagtc gcgcatttcc tgaaggcctt ccatgctgtt cttgccgatt
     2821 tccaaaatgc acggtgtgta caattggata taggtcggtc caatttcacg cgcaatcaag
     2881 accgagtttc ggatcacctt ttctacgagt gacggcttgc tcactgtgca gttcactacg
     2941 tagtgacagc cggactctcg ggcaatttca ggcaaacgga ccttatcgaa cagtttccca
     3001 accggggcca tttttgcaac gaagcccttt tgcatgaggc cgctttcctg gccgccggta
     3061 ttggcataca gctcattatc gaaacagatg ggcgtgaatt tctcctggcg gaaccaggcc
     3121 tgcaaggtca tgtcgaggcc gatatcgacc gtggcgccgt cgcccgctaa gacgaccacg
     3181 tctttcacgc ggtcggggaa gcgcacgctg agggctcgtt tcagcccgga tgcgattgcg
     3241 ttttggttgc cgaagaggga gtgaatgttg tgtacggcca ccatcgggaa caccaggctg
     3301 gtacagccgg tggagccgac catgaccgtg tcttccgggt tgggaaggga ggccaggata
     3361 taacggaaag ccattgattc agggcaaccg gcacataagg aatgttgttc gatcagttcc
     3421 ttcgaggttc caatatcctt ccagccgcgg tcctctttac cgtaggtcgc actctggacc
     3481 aaatcttggt agtccgaggg catgatgtca tataggtccg gcgaaatttt gattcgctct
     3541 ttactcatgt atgcctcctc aaggctgttc tatcggatag taggtgtatg gctcttcagt
     3601 tcactattat tttcagctgc ctcttccagc aagcgattct gaacgcaggc cgagcgccgt
     3661 cttgatctca gacacaatga tttccggtgg catcgtcatg ccgccgcaca catgcgggcc
     3721 agcatgtacg cgttcgctgt tgggaatggt ggccttaatt tcctttgcca gccatcccgt
     3781 cacattgaac tccggcacaa agatgtgctt ggcattcttc gtagcctcac gaatttcctc
     3841 tcctggccag gggcggagtg atttcacctt caccaagccg cagcgcatgc cttcatcctc
     3901 aaggaggcga atggcctctc gaccttgtga gactgccgtg cccgacgcaa caatgacgac
     3961 ctccgcatcg gtgttctctg tgtcgatcaa gccattgatc cacttgatcg tgtgtttgcg
     4021 tgaccgttcc actgccgccc aaatttcctg ttgccaggaa gcgtgggttg cgtagctgat
     4081 gtagttgctc ttcatgatga aaggatcgcg catcatgcgg accggtggac actccatgtc
     4141 catacagggc acaggcgagc gatagggatc gtaaggcggg aggcacatat cagccggtgt
     4201 cagattgacg acgtctttgg tgtgggtcac gaagaagcca tcgcaacata acgccaacgg
     4261 caaatgcact tccggctctt ctgacaccat gaagcctttg aggatccaat caaaaaagtc
     4321 ttgcgccgtc tccgcatgcc aaacaagcat ccctgtattc aaaagatacg aaatttcaat
     4381 ggtatcaggc tgaatcgaga gcggtgagtt gatgccacgg catgtgacga tcatttggat
     4441 aggcaagcgc gcaccggccc acatggggaa gttttccatg gcacgcatcg ttccaggccc
     4501 agccgtcgtc gtgaacacgc gcgctccgcc gaaggcggcc ccggcgcatt gggacatgac
     4561 ggcaaactca ctctcaccac ggaaataatc accgatataa ccctcggcaa acagttcgcc
     4621 gataagggct gcggcttcgc tctgcggggt gatcgggtac gcgatcatta cgtcgcagct
     4681 cgcgcgccga agggcttcct taattacttc gctgccggta taaaaagacg gagtgcgggg
     4741 ggcttcctgc atcatcttcc aggcatctgt gaacgtctgg ccttttttat tctgtgtccc
     4801 gatgtgggat tttatttccg ccatggagtc ggcctccttt caatcgttag aagagcttta
     4861 aatcacttag ttggaagcgc tggtcttggc tgatgtcagc cgtggtttta ccgtgccctt
     4921 gatcttgcgg atccactcgc ccagtttcaa gattttttca acagaggcat ctctgaaaga
     4981 gagcatggca tcttcatgtc ctgcttgggc acacatcgcg attgtgtaac atgacgttcc
     5041 accccggata atcttaaggg acaaattcgc ctggtggcag tgtaccccga tgaacatgca
     5101 gcagtcgatc ttgttatgcc aaatggtcag gttcgggtgg ttgggattaa tttccacttc
     5161 aggattgatc ttctgatact ttgggcgata gtccggcatc ggaatcagca tcggcttttg
     5221 tcctggctga acgcactcct tcagaatttc atat
//
LOCUS       DAORQN010000100         5216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_643_length_5216_cov_13.124588, whole genome shotgun sequence.
ACCESSION   DAORQN010000100 DAORQN010000000
VERSION     DAORQN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_5216_cov_13.124588"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PK614_06455"
     CDS             <1..821
                     /locus_tag="PK614_06455"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRB81889.1"
                     /translation="FAGVDIAFVSATDTISRDYGARLGAGGVVVIDDSAVFRMDPDVP
                     LVVPEVNAAALRSMKRGIVAIPNCTTTPLVMALKPLRDIAGIKRVVVTTFQSVSGTGS
                     AAMDELVDQTKALMAFQDVKVQVYPYQIAFNLLPQVGSFGDGGDCSEEVKIVQETRKI
                     LEMPSLRVTATTVRVPVLRCHSEAINVELERPLKANDARAALAEMPGVIVYDDPVKKL
                     YPMPFDVSGKDDVYVGRVRVDESITNGLNLWVVSDNLRKGAALNAVQIAECLIQ"
     gene            825..1079
                     /locus_tag="PK614_06460"
     CDS             825..1079
                     /locus_tag="PK614_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRB81890.1"
                     /translation="MAAWTGIGRSLIVAGLVLVAVGLLLTLAEKWPGLGSAFGWIGKL
                     PGDFSVTRERFSLYVPIATSLVFSILLSLVFYFLSWLFRR"
     gene            1061..2272
                     /locus_tag="PK614_06465"
     CDS             1061..2272
                     /locus_tag="PK614_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRB81891.1"
                     /translation="MALSPLTRHQSSSLAVAGGMLLLALFCVPAVFAESIRVLLAQEA
                     PLVEVRSEGALALVTETGDTKILQAPIHLTARGDGVHVDGRRSMGGQVLIRPLRNNLS
                     LVIGKEGGATAGAPLALSGVVRLSRKGHGLSVVNQVDLEEYVKGVVPSEVSSAWHPEM
                     LKVQAVAARTYALYNKMLSAARDYDVVATIQDQVYRGRTGVDRRVEDAVESTRGIVVT
                     HQQAPIYAAFSSTAAGPTEDAVNVWANKDLPYLKGVECPFDLESPYYQWRASVKIDQL
                     EHNLRHQGFSVGTIATITPIAYSRAGRVSRLRILHSSGETVLRGEDLRKAVGYTVIPS
                     TQFEVESIGAEVVFAGYGAGHAVGLCQWGAKELAELGYSYSSILQYYYPGTELHNAAL
                     SQLMMPVIPTP"
     gene            2272..3312
                     /gene="queA"
                     /locus_tag="PK614_06470"
     CDS             2272..3312
                     /gene="queA"
                     /locus_tag="PK614_06470"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRB81892.1"
                     /translation="MLLSEFDFPFDPVLVADHPVIPRDRARLLVLDRQGGERVHRQVA
                     DLPSLLQSGDLVVVNNTKVMPARVPAQVRSNGKLLDLLFVEDLGQGLWEVLIKGRFRP
                     GAVIEFPGGGTGEIVERSRLRTTVQVSGVASVYELMQATGTMPLPPYIKREPSADDQE
                     WYQTMFAQHEGAIAAPTAGLHFTRELVQALAGKGIELAQVTLHVGPGTFRSVKTERIE
                     DHRMLAERIEVSAQTVAQIRLTRERGNRVVAVGTTVVRALETAARGGRGIEPFQGPAE
                     LFITPGFEFQVIDAMVTNFHLPRTTLLMLVSAFVGVQPLRAAYEEAVAQRYRFYSYGD
                     AMLIGSGWATVT"
     gene            complement(3305..4003)
                     /locus_tag="PK614_06475"
     CDS             complement(3305..4003)
                     /locus_tag="PK614_06475"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRB81893.1"
                     /translation="MIKLLREAAHDYPWFLKSIMGLLALAFVITMGWWGFGQEGGNVV
                     ASVGDQVVPLDEYRRAYENTYRFYKDKGQNDIKDEFLKQFVLEQLIDNRMWLHVAKEM
                     GLTVSDEDLRKAIMQRTEFQKNGNFDPEAYRRLLSANRLTPASFEAMETKDILTNKAR
                     LVIMDAVALTPAEYTEAQTLAAREGDADPAKAAAAKERVFQSLLFQKQQRALMAYAES
                     MKSKVPVKIHKELM"
     gene            4077..4622
                     /locus_tag="PK614_06480"
     CDS             4077..4622
                     /locus_tag="PK614_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRB81894.1"
                     /translation="MNGARGKIATLFVQVKEICYSVEELERSPVVEEAIGHSPLELGV
                     YPKAQVLNRFIAKMIDLLIVAAVSKLVPPIGVLAGLAYLLLADGFGGGRSVGKRLIGL
                     QTIVPRTRDPAGFRESIIRNLPCGLAQLAFEIPYVGWIGWGAVLSLEGLLVIGNEQGR
                     RLGDEIAKTQVLESGQLDVSD"
     gene            4680..>5216
                     /gene="mreB"
                     /locus_tag="PK614_06485"
     CDS             4680..>5216
                     /gene="mreB"
                     /locus_tag="PK614_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HRB81895.1"
                     /translation="MSDMFGWFSNDLAIDLGTATTLVYVQGKGIVLNEPSVVAVEKKT
                     ERVMAVGADAKRMLGRTPGNILAVRPMKEGVIADFEKAEAMLSHFIQKAHNRTAFVRP
                     RIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMVVDI
                     GGGTTDIAVISLGGIVYSE"
BASE COUNT          970 a         1397 c         1735 g         1114 t
ORIGIN      
        1 cctttgccgg agtcgacatt gcgttcgtgt ccgcgaccga taccatcagt cgggactacg
       61 gggcgcggtt gggcgcgggc ggggtcgtcg tcatcgatga cagcgcggtt ttccggatgg
      121 atcccgatgt cccgctggtc gtcccggagg tcaatgcggc agcgttgcgc tcgatgaagc
      181 gcggaattgt ggccattccg aactgcacca caaccccctt ggtcatggca ctgaagccgc
      241 ttcgcgatat tgcaggcatc aagcgggtgg tggtgaccac ctttcagtcg gtatcgggca
      301 ccgggtcggc ggccatggat gaactcgtcg atcagactaa ggccttgatg gctttccaag
      361 atgtgaaggt gcaggtctat ccctatcaga tcgcgttcaa cctgttgccg caggtcggct
      421 cattcggtga cggcggtgac tgttcggaag aggtcaagat cgttcaagag accaggaaga
      481 ttctggagat gccgtcgttg cgggttacgg cgaccacggt gcgggtgccg gtgttgcgct
      541 gtcactccga agcgatcaac gttgagctgg aacgtccgtt gaaggcgaac gatgcgcggg
      601 ctgcgctggc ggagatgcct ggcgtgattg tgtacgacga tcccgtcaag aaactgtatc
      661 cgatgccgtt tgacgtgtcc ggtaaggacg acgtgtatgt cgggcgggtg cgtgtggacg
      721 agtcgatcac caatgggttg aacctctggg tcgtgagcga caaccttcgg aagggcgccg
      781 ccctcaatgc cgtgcagatc gccgaatgtc tgatacaatg aacgatggca gcgtggactg
      841 gtatcggtcg atcgttgatt gtggcgggcc tggtgttggt cgccgtcggg ctgctgctca
      901 cgctggcgga aaagtggcca ggccttggct ctgcgttcgg gtggatcggt aaattaccgg
      961 gcgacttctc ggtcacgcga gagcgattca gtctgtatgt cccgatcgcg accagtctcg
     1021 tgttcagcat cctgctgagc ctggtgttct acttcctttc atggctcttt cgccgctgac
     1081 tcgacatcag tcatccagcc tggccgtagc ggggggcatg ttgctgctcg cgctgttctg
     1141 cgtaccggcc gtctttgccg aatccattcg cgtcctcttg gcccaagagg cgccgttggt
     1201 ggaagtgcga tcagagggcg ctctcgctct ggtgacggag acgggcgaca cgaaaatcct
     1261 gcaagcgccg atccatctca cggcccgagg agacggcgtg catgtcgatg gccggcgcag
     1321 catgggcggg caggtcctca tccgtccgct gcggaacaac ctctcgctgg tgatcggcaa
     1381 agaggggggc gcaaccgcgg gagctccatt ggctctcagc ggggttgtgc gtctgtcgcg
     1441 caaagggcac gggctttcgg tcgtcaacca ggtcgatttg gaggagtatg tgaagggggt
     1501 cgtgccgtcg gaggtcagtt cggcctggca cccggaaatg ttgaaggtgc aggccgtggc
     1561 ggcacggacc tatgcgctct ataacaaaat gctcagcgcg gcacgggact atgatgtggt
     1621 ggcgaccatt caggatcagg tgtatcgagg ccgcacagga gtagaccgcc gagtcgagga
     1681 tgcggttgaa tcgacgcgag gcattgtcgt cacccatcaa caggcgccga tctatgccgc
     1741 gttctcatcc accgcggccg gcccgacgga agatgccgtg aatgtctggg cgaacaaaga
     1801 cctgccctac ctcaaggggg tcgaatgtcc attcgatctc gaatcgccct actatcaatg
     1861 gcgggccagt gtgaagatcg atcaacttga gcacaatctg cggcatcagg gtttctctgt
     1921 gggaacgatt gccacgatca ctccgattgc ctacagccgg gccggacggg tctcgcgcct
     1981 gcggattttg cactccagcg gagagacggt attgcgtggc gaggatcttc ggaaggccgt
     2041 ggggtacacc gtcattccca gcactcagtt cgaggtggaa tcgatcggtg ccgaggtggt
     2101 gtttgccggc tatggagcag gccatgccgt ggggctctgc caatggggcg cgaaagagtt
     2161 ggccgagctg ggctattcgt acagcagtat tctgcagtat tactatcctg gaaccgagtt
     2221 gcacaacgcg gcgctctcgc agttgatgat gccggtgata ccgaccccct gatgttactc
     2281 agcgagttcg atttcccctt tgatccagtg cttgtggccg atcacccggt gatcccgcgt
     2341 gatcgcgcgc gtttgcttgt gctggatcgg caggggggcg aacgcgtgca ccggcaggtg
     2401 gctgaccttc catcattgct gcagtcgggg gatctcgtgg tggtgaataa caccaaggtg
     2461 atgccggccc gtgtgcccgc gcaggtccgg tcgaacggaa aactcctcga cctgctgttt
     2521 gtggaagatc tcgggcaggg gctctgggag gtgctgatca agggacgctt tcggccaggc
     2581 gcggtgatcg agtttccggg cggcggtacc ggagagattg tggagcgcag ccggcttcga
     2641 acgaccgtac aggtgtcggg tgtcgcaagc gtctacgaac tcatgcaggc cacgggcacg
     2701 atgccgcttc ctccgtatat caaacgggaa ccgtcagcgg acgatcagga atggtaccag
     2761 accatgtttg ctcaacatga aggggctatt gcggcgccca ccgccgggct acattttacg
     2821 cgcgagttgg tgcaggccct ggcgggaaag ggaatcgagc tggcacaggt gacgttacac
     2881 gtcggccccg gcacgtttcg cagtgtgaag accgagcgaa tcgaagacca tcgcatgctg
     2941 gccgaacgaa tcgaggtgtc ggcgcagacg gtggcgcaga ttcgcctgac gcgggagcgg
     3001 gggaatcgtg tggtggcagt gggcactacg gtcgtgcgcg ccctggagac agcggcgcga
     3061 ggcgggcgcg ggattgagcc cttccaggga ccggccgaac tgtttattac cccggggttt
     3121 gaatttcagg tgatcgacgc gatggtgaca aactttcatt tgccgcggac caccctgctg
     3181 atgctggtgt ccgccttcgt gggtgtgcaa ccgctccgcg ccgcctatga agaggcggtg
     3241 gcgcaacggt atcgattcta tagttatggg gatgcgatgc tgatcgggag tggctgggcg
     3301 accgttacat aagctctttg tgaattttca cgggcacttt gctcttcatc gactccgcgt
     3361 aggccatcaa cgcccgttgc tgcttctgaa acaggaggct ctggaaaacc cgctccttgg
     3421 ccgcagcagc cttggccgga tcggcgtccc cttcgcgagc ggcgagtgtc tgtgcttccg
     3481 tatattcagc cggcgtgagc gccacagcat ccatgatgac cagccgcgct ttgttggtga
     3541 gaatatcctt ggtttccatg gcctcgaatg aagcgggggt caaacgattt gcggacagca
     3601 atcgccggta cgcctcggga tcgaagttcc cgttcttctg aaactctgtg cgttgcatga
     3661 tcgctttgcg caagtcttcg tcggagaccg tcaggcccat ctctttcgcc acgtgcagcc
     3721 acatgcgatt gtcgatgagt tgctccagca caaactgctt cagaaactca tccttgatgt
     3781 cgttctgtcc cttgtctttg tagaagcgat acgtattttc ataggcccgg cgatattcat
     3841 ccaaggggac gacctggtct ccgacggagg cgacgacgtt gccgccttcc tggccgaagc
     3901 cccaccagcc catcgtaatg acgaacgcga gggcgagcag gcccatgatg gatttgagga
     3961 accatggata gtcgtgagca gcttcgcgta acagtttgat catcgagcgc gcctcttgtc
     4021 ggcaaggttt cgcggattct actcaggcca cgatcacaaa ggcaagagga agacgcatga
     4081 acggcgcgcg cggtaagatt gcgactttgt ttgtgcaggt gaaagagatt tgctatagtg
     4141 tcgaagaatt ggagagatct ccggtggtgg aggaggcaat cgggcacagc ccgctcgaac
     4201 tgggcgtcta tcccaaggcc caggtgctga atcgcttcat tgccaaaatg atcgatctcc
     4261 tgatcgtcgc ggcggtgagt aagttggttc ctccgatcgg tgtcctggcc ggacttgcct
     4321 atctcttgct ggccgacgga ttcggcggcg gtcgcagtgt cgggaagcgg ctcatcgggc
     4381 tccagaccat cgtgccccgt acccgggacc cggcagggtt ccgagaatcg atcatccgta
     4441 atctgccttg cggtcttgcc cagttggcat ttgaaatacc gtatgtcgga tggatcggat
     4501 ggggcgccgt gctctcgctg gaagggttgc tggtcatcgg gaatgagcag ggccggcgat
     4561 tgggcgatga gatcgccaaa acccaggtgc tggagagcgg acagctggat gtgtcggatt
     4621 aagcgggacg gtgtggttga tcgcaggaat cgtttgaagg gagagtccta gtgggcttca
     4681 tgagtgacat gttcgggtgg ttctccaacg atctggcgat cgatctgggg acggcgacaa
     4741 ctctcgtgta tgtccaggga aaaggcatcg tcttaaacga gccttccgtg gtcgccgtgg
     4801 agaaaaagac ggagcgtgtg atggcggtcg gcgccgacgc gaagcgcatg ttggggcgca
     4861 ccccggggaa tatccttgcg gtacgtccga tgaaggaagg cgtcatcgcc gatttcgaaa
     4921 aggccgaggc gatgctcagc cacttcattc agaaggcgca taaccgaacg gcgtttgtgc
     4981 ggccccgcat catcatcggt gtaccgtcgc ggattacgca ggtagagcaa cgggccgtcc
     5041 gtgattcggc cgaactggcg ggagcccgcg aagtgtattt gatcgaggaa ccagtcgcgg
     5101 cggcgattgg tgcagggttg ccgattaccg aaccgtccgg gaacatggtg gtggatatcg
     5161 gcggtggtac gacggacatc gcggtcatct ccctgggcgg catcgtgtat agcgag
//
LOCUS       DAORQN010000101         5189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_647_length_5189_cov_4.494741, whole genome shotgun sequence.
ACCESSION   DAORQN010000101 DAORQN010000000
VERSION     DAORQN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_5189_cov_4.494741"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..969)
                     /locus_tag="PK614_06490"
     CDS             complement(52..969)
                     /locus_tag="PK614_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81896.1"
                     /translation="MKAQAPFLEVRSFVTEEINQDALETTAPPSTPFLSLYESEEGGA
                     WVDPETEEHVAFLNELYDEEFDEALCALVDEAAAIYEAQFPYEQEDPRTVGYQAERLL
                     TQHFAPLVAEAEAMFTALAREFSQRDPNVLSENEIETIVDRCRPSAELTPTFEEFFGK
                     LKKLAKNVAKKAVGLAKKGVQFAAKLGLGPILNKLKALIKPLLQRVIQTAIGKLPPHL
                     QPIAQKLAERLPFLKKPQESGGAVAEPAEPASEPAAEPGTEPSEPSQVADIQSEFHHE
                     VTNLLFARTEVEQDLEVARVLTERPVPDT"
     assembly_gap    1167..1176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1283..1675)
                     /locus_tag="PK614_06495"
     CDS             complement(1283..1675)
                     /locus_tag="PK614_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81897.1"
                     /translation="MPEGNLESMLPISILLVGVRPRWVGELFKRRTDIKIVDEISRVK
                     DLGKAMAQKECDVVVTALPDGQVQPVLSSVLFGPGHTAIVAITARGHNIEVYDHRVRR
                     EVAPEDLIRVIREVVHRKRLAGNSITQE"
     gene            complement(1848..2657)
                     /locus_tag="PK614_06500"
     CDS             complement(1848..2657)
                     /locus_tag="PK614_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB81898.1"
                     /translation="MRKARQTKADTSDKSIRSHASVPATKAVTLSMRTPTPNSPERID
                     PHPQIRVVVLGDVRLYREGLAQLLGHRDTLHVVGVAPVVPEGLNLVTVECPDIVLVEA
                     AVVYKRETLRKILEVAPTIKCIAYGIVDEEREAIECAEAGAIGYVSYEATVEDLVATI
                     LSAAHEEFSCSPRITAILLRRLSTVAAKQQDEGSEALLTVRERQVLALVDQGLSNKEI
                     ATRLGIEVSTVKNHMHNVLEKLHATCRGEAAARVRHAFLSPAIQKHSFSQI"
     gene            complement(3097..3513)
                     /locus_tag="PK614_06505"
     CDS             complement(3097..3513)
                     /locus_tag="PK614_06505"
                     /inference="COORDINATES: protein motif:HMM:NF018826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HRB81899.1"
                     /translation="MNSELVLQASHATLAGSLPFPDIVRQLLEAGVEYYHVDYILLQF
                     TFYGANAASVVAPLTFEGLPAVAKDFDAATLRQAILDSQQKGQQFREFSQRAMRAGVQ
                     GYFAFLKGTRVTYLGRQGDQHTEWFPGAERKANLRE"
     gene            3629..>4044
                     /locus_tag="PK614_06510"
     CDS             3629..>4044
                     /locus_tag="PK614_06510"
                     /inference="COORDINATES: protein motif:HMM:NF028174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HRB81900.1"
                     /translation="MPENNHNSAPLPPPHVQLIQMAMAHWVSRIAYVAAKLGLADHLA
                     NGPKTADELAGPTGTHAPSLYRLMRTLTNLNILAEDTTHRFALTPLGEALKTGAPGSA
                     RPSILTLASNWMSRGWEQLLYSVQTCGGGFEQSLGM"
     assembly_gap    4045..4078
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4079..4641
                     /locus_tag="PK614_06515"
     CDS             <4079..4641
                     /locus_tag="PK614_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB81901.1"
                     /translation="MGFHGAEPAAITAAYDFSGLSTLIDVGGATGHLLTTILAGNPAS
                     RGILYDLPHVVRDAPALIQARGLADRVTIEVGSFFDRVPSGGNAYLLSHIIHDWTEAQ
                     CLTILGHCRRAMTPGSRLLIIEMVLPSDNGPHPGKMLDMMMLVGPGGQERTESEYREL
                     LGKAGFRLTRLVPTETAVSVVEAVLA"
     gene            4741..4995
                     /locus_tag="PK614_06520"
     CDS             4741..4995
                     /locus_tag="PK614_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRB81902.1"
                     /translation="MAHLPTSKAREGFADTINRVAFGKERVILRRRGKEVAAVVPIAD
                     LRLLEDLEDRIDLADARAALAETKKKGAKPLDVILKELGL"
     gene            5005..>5189
                     /locus_tag="PK614_06525"
     CDS             5005..>5189
                     /locus_tag="PK614_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB81903.1"
                     /translation="MAYSILLAPSAERQLKALAGPIQKRIVKRLGTLRTNPRPQGVKK
                     LAGEDDLYRLREGDHRI"
BASE COUNT         1123 a         1484 c         1391 g         1147 t
ORIGIN      
        1 ggtgaagcca ggctaccact taacacactg tccgaatttc tggagccctc tctaggtatc
       61 aggaaccggc cgttcggtaa gcaccctagc gacctccaaa tcctgctcca cctcagtgcg
      121 agcgaacagg aggttggtga cctcgtgatg aaactcagat tgaatgtctg ccacctgaga
      181 gggttccgaa ggctctgttc caggctcggc tgcgggctct gatgcaggct cggcaggttc
      241 tgccactgcc ccaccactct cttgtggttt tttcaagaag ggcagacgtt ccgccagctt
      301 ttgggcgata ggttgaagat gaggaggcag cttgccaatt gccgtctgaa tgactcgttg
      361 gagcaacggc ttgatcagcg ccttgagctt gttgagaatt ggtccaaggc ccaacttggc
      421 agcgaactgc acccctttct ttgccaggcc aacggccttc ttagccacat tcttggcgag
      481 ctttttgagc tttccgaaaa actcctcgaa agttggcgtg agttcagcgg atggtcgaca
      541 ccggtcaaca atggtctcga tctcgttttc gctaaggaca ttgggatcgc gctggctgaa
      601 ttccctggcc agagctgtaa acattgcttc ggcctccgca acgagagggg cgaagtgctg
      661 cgtgaggagt cgttctgcct ggtaccccac ggtccgggga tcttcctgtt catatgggaa
      721 ctgtgcctcg taaatggccg ccgcttcatc taccaaagca cacagcgcct catcgaattc
      781 ttcgtcatag agctcattga gaaacgcaac gtgctcttcg gtctccggat cgacccaggc
      841 gccgccttcc tcggattcgt aaagcgacaa gaatggggtg ctgggaggag ctgtcgtctc
      901 gagagcatct tgattaattt cttccgtcac gaaactgcgg acttctagaa aaggggcttg
      961 agccttcatg agcttgtctc cctagagttt gttttttgct atcatgttag gaatggtcct
     1021 tcatgactga gatcgaagaa atggacagac tccggtcaaa acgaatgggc ctattggctg
     1081 cgcccgaggc agagaacagc aagagaggag tatcgacttt gaccccaaag ggcctgccgg
     1141 gtctagctgg cggaactggc cattttnnnn nnnnnncact aagacacgcg cattgctcac
     1201 gaacaggctc tcaggctcca acggaattgt gccggtactg ccttcagtgg agaagagggt
     1261 ctcttcctgt gccatgatca actcactcct gtgtgatgct attacctgct aagcgcttgc
     1321 gatggacgac ctcacggatc acacgaatga gatcttctgg cgccacttca cgcctaaccc
     1381 tatggtcgta cacctcgatg ttatgcccac gagcggtaat cgcgacgatt gccgtatgac
     1441 ccggtccgaa tagcaccgag ctaaggacag gctggacctg cccatcagga agggctgtaa
     1501 ccaccacgtc acactctttc tgagccatgg ccttgcccaa gtctttaact ctagagattt
     1561 catcaacgat ctttatatct gttcggcgtt taaagagttc gcctacccac cgaggacgaa
     1621 caccgacgag caggatactg atcggcaaca ttgactccaa gttcccttca ggcatggccg
     1681 gatgctagaa cgaagcctca ttaaagggaa tggatcaggg gattcaaagg agattgggtt
     1741 gcagatctag accctctata gtcagtctta agccacgtcg gtgacccacg acttcccacg
     1801 agcgctgcat gacagctttt ctcgcgccgg gtcctggatc agaggaacta gatctgactg
     1861 aacgaatgtt tctggatagc agggctcagg aatgcgtgac gcacacgcgc agctgcctcc
     1921 ccccgacacg tggcgtgcag tttctcaagg acgttgtgca tatgattctt gacggtggac
     1981 acctcaatgc ctagacgggt ggcgatttcc ttgttggaca agccttggtc aactaatgct
     2041 aaaacttgcc tctctcgcac ggtaaggaga gcctctgatc cttcatcttg ctgttttgcg
     2101 gcaacggtgg ataagcgccg aagcagtatc gctgtgattc gcggcgaaca ggaaaattcc
     2161 tcatgcgctg cgcttaaaat tgtcgccacg agatcttcaa ccgttgcttc atacgatacg
     2221 tatcctattg caccggcttc tgcgcactcg atcgcctcac gttcttcatc tactattcca
     2281 taggcgatgc atttgatagt aggcgcaacc tctaggatct tccgcagggt ctctcttttg
     2341 tagacaactg cagcttcgac cagaacaatg tccggacact cgactgtgac caaattcaag
     2401 ccttcaggaa cgacgggcgc aacgccgact acgtgtaaag tgtcacggtg accaaggagc
     2461 tgagccaagc cctctcggta taagcgcaca tccccgagaa caactacccg tatttgtgga
     2521 tgaggatcta tcctttcggg gctgttcggc gtaggcgtgc gcatcgagag agtgacggct
     2581 ttagtcgcgg gaacggaagc atgtgaacgg atgctcttat cgctggtgtc agcttttgtt
     2641 tggcgggcct tgcgcatgag cacaccttat gtagtcactg aagcgggttg gtaggattgt
     2701 tctgtaaagt tttcctctca atcgggtatt tcaacaactt gagcaacaag cgtaccgtat
     2761 cggtgactgc cgcgatttcc aaagaaacaa acctttcatc catagtgatc ctcagaatgc
     2821 atgtatccat accgatacat gtgtgtatcc atttcggtcc attctcccgc tgcgcccatc
     2881 agtagtggat aagcccagaa gtggcctcgg aaatttgaac aagcggttag gtgcatcaaa
     2941 caggatcttg caatcgcaca tctcctgtgc tcccctccag ccgcgtcact cccttcggcc
     3001 gctcgagaga cttgctcgcg tgtacatcca ggcttcatcc ggctcaagcg aactgactga
     3061 ggcatcacgc ttacggatca cgagccggac gttcattcac tcgcggaggt tcgctttgcg
     3121 ctccgcaccg ggaaaccact cggtgtgttg atccccttgc ctcccgagat aggtcacgcg
     3181 tgtgcccttc agaaacgcaa agtagccctg cactccggct cgcatggcgc gttgtgagaa
     3241 ctcacgaaac tgctgccctt tctgctgact atccagaatc gcctggcgta acgtggccgc
     3301 atcaaagtcc ttcgcgaccg ccggcaaacc ttcgaaggtc agcggagcga cgacgcttgc
     3361 ggcgttcgcc ccgtagaatg tgaactgcaa caggatgtaa tccacatggt agtactcgac
     3421 accggcctcg agaagctgac gcacgatatc cggaaacggc agtgaaccgg cgagcgtggc
     3481 atgactggct tgcagcacaa gttcggaatt cacgcgtcct ccttccggga gagaaaagga
     3541 atttaacgga gcgaacatac cgtgtataac tctcgctcaa caacatcgga aatgtccgtc
     3601 tttcacatcc taaccggagg aaaccggcat gcctgagaac aatcacaaca gcgctccgct
     3661 gccacccccg cacgtccaat tgatacagat ggccatggcc cactgggtct cccgcattgc
     3721 ctacgtcgcc gccaaactgg gcttggccga ccaccttgcg aacggaccga agactgccga
     3781 cgaactcgcg gggccgacag gaactcatgc gccctctctc tatcgcctga tgcggacgct
     3841 cacgaacctc aatatcctcg ctgaggacac gacgcaccgc tttgccttga cgccgctcgg
     3901 ggaggcgctc aagaccggcg caccgggatc ggcacggcct tcgatcctga cactggcaag
     3961 caactggatg tcgcgtggct gggagcaact gctctattcg gtgcagacct gtggcggcgg
     4021 atttgagcaa tcgctgggga tgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     4081 atggggtttc acggggcgga accggcagcc atcaccgccg cctacgattt ttccggcttg
     4141 tcgaccctga tcgacgtggg cggggccacc ggacatctcc tgacgacgat tctggccggc
     4201 aatcccgcat cacgcggcat cctttacgac ctgcctcacg tagttcgtga cgcaccggcg
     4261 ctgattcagg cccggggcct ggctgaccgc gtgacgatcg aggtcggcag tttttttgac
     4321 cgtgtgccga gcgggggcaa tgcctatctg ctgtcgcaca tcatccacga ttggaccgag
     4381 gcccaatgcc tcaccattct cggccactgc cgccgcgcga tgacacccgg cagccgcttg
     4441 ttgatcatcg agatggtgct gccgtccgac aacggtccgc atccgggcaa gatgctcgat
     4501 atgatgatgc tcgtgggtcc aggcggacag gaacggactg aatcagagta ccgtgagctc
     4561 ctcgggaaag cgggattccg tctcacccgg ctcgtgccga cggaaacggc cgtgagcgtc
     4621 gtcgaagcgg tgctcgcata aactctgcgc ctgccttttt ttcgttcctt gacttcccag
     4681 tccgtcaaac gtacacttgg tacaattggt acgtttcgta cagttctgat gaggtcactc
     4741 atggcgcatc tgccgaccag taaggcccgc gagggattcg ctgatacgat caatcgcgtc
     4801 gcattcggca aggagcgtgt gatcttgcgg cggcgcggca aagaagtcgc cgcggtggtc
     4861 cccattgcgg acttgcgatt gctggaagat ctggaggatc gcatcgacct cgcggatgcc
     4921 cgagcggcgt tggccgaaac gaagaagaaa ggcgccaagc cactcgatgt cattctgaag
     4981 gaactcggcc tctaaaattt cgcgatggcc tattcgattc tcctcgcccc ctctgccgag
     5041 cgacaactca aagcactcgc cgggccgatt caaaagcgaa tcgtcaaacg ccttggcaca
     5101 ttaaggacaa atcctcgccc tcagggagtc aagaaactcg ccggcgaaga tgacttatac
     5161 cgacttcgag aaggcgacca ccggatcat
//
LOCUS       DAORQN010000102         5185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_649_length_5185_cov_44.400585, whole genome shotgun sequence.
ACCESSION   DAORQN010000102 DAORQN010000000
VERSION     DAORQN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_5185_cov_44.400585"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..1304
                     /locus_tag="PK614_06530"
     CDS             528..1304
                     /locus_tag="PK614_06530"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HRB81904.1"
                     /translation="MSDAERETLSLGLAHGHSLKTMAAVLGARPNTVSRESARNAARG
                     RPYRACTAQTLAAARARQPRRSRKLLAPWLWQYVRTHLVRGCSPEQIAGRLRREYPDD
                     MQKRLSAETIYVGLYVLPRGTLRSALLAALRQARKARRPRSRGTDRRGHIPNMTPIAA
                     RPAEVANRTVPGQWEGDLIKVARNGSAVGTLVERTTRLVILARMAGTDAGRCLRGVHK
                     ETPTRAGAAAQDPGLRSGQRDGGTRASGPAARHSGVLCRS"
     gene            1365..1643
                     /locus_tag="PK614_06535"
     CDS             1365..1643
                     /locus_tag="PK614_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB81905.1"
                     /translation="MPRKTYTPEEIIQHLRTVELETGKGIAVVEACRKVGITEQTYYR
                     WKKEYGGLRVDQAKRLKALEQENLRLKRIVADQAVDLSILKEVASGNF"
     gene            1667..2080
                     /locus_tag="PK614_06540"
     CDS             1667..2080
                     /locus_tag="PK614_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB81906.1"
                     /translation="MRHAMAGLHVSERRACRAIGQLRSSYCYVSQPDPTDERLWVRVI
                     ELAKEYGRYGYRTITWLLQREGWDVGKDRVYTIWRHEGLKVPAKQPKRARLWRADGSC
                     VRLRPTHRHHVWSYDFVAERTHDGRPFRILNVLDE"
     gene            complement(2095..3224)
                     /locus_tag="PK614_06545"
     CDS             complement(join(2095..2972,2972..3224))
                     /locus_tag="PK614_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611579.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB81907.1"
                     /translation="MTRKRHTEEQIIAVLKDAQAGIGVHELCRKHGISDATFYKWRTK
                     YAGLEVSDVKKLRQLEDENRRLKQMVAEQALDIQALKAVTGKKLVAPRAKRTAAQWMA
                     ERFGLSQRRVCHLLALDRNTLRYRSRRQEDAALRTRIREIAETKRRYGCPRIYVRLRR
                     EGWRVNHKKVERLYYRDEGLSLRRRRRKKAAAVPRVALSKPTQPGRCYAMDVVHDRLV
                     RGRRFKCLTRTDLYSKEVPVIEVDVSIGGARVCRVLDRLFNTRPLPDTVILDNGPEFA
                     GTALEAWAVQHGVHLHFIQPGKPVQNAFIESFNGKFRDECLNEHWFLTLQEAQLVIEA
                     WRREYNEERPHSAIGNLTPREFINHHHNQPQIAQESTSLAVV"
     gene            complement(3300..3461)
                     /locus_tag="PK614_06550"
                     /pseudo
     CDS             complement(3300..3461)
                     /locus_tag="PK614_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3643..4686)
                     /locus_tag="PK614_06555"
     CDS             complement(3643..4686)
                     /locus_tag="PK614_06555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB81908.1"
                     /translation="MAPAQPASPSTRLVHLDNYRGLAIAMVVATHALGYAHLPGDEQY
                     QIAWMVQTIAVPVFFLVDGLLFGIRHGAKTAFDYREHLAKSMHRLLIPWALFTLLYGV
                     LRLMFELTGSAPAHILVGRPWQDWITAIYLSDFSSQMYFLLSLFFIRAAAPPLHILAQ
                     CGLPIRVLAVIVYLGAFHLLPLRTWFFPGLDPIYHALWGLQFYLIGLVIPLVSGFLAT
                     RGIALSLVTLAGGILLPILSDHTVIPAQFLLLIGSYIAFASTTFSLRVLSTLGRYTMG
                     IYLLHIPLVMKGMALIFSRILPPTSLSFFFCLAFFSLITSLFLTKMLLQLPFGRQALG
                     EQAQGPGPGTHAK"
     gene            complement(4746..>5185)
                     /locus_tag="PK614_06560"
     CDS             complement(4746..>5185)
                     /locus_tag="PK614_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81909.1"
                     /translation="GGLWHGAAMHFVLWGGFHGLLLMLTPQRSQAGPPRTLLGTVLRR
                     LGWFQLIAFGWLLFRVQDMKNFMDFIEGCARFTGGTRLSGVFYLILCAGILSHLSPLT
                     QLERFGEWWIARPVPIQAAAYAVLLIFYAGVTLEAPAFIYFQF"
BASE COUNT         1095 a         1521 c         1578 g          991 t
ORIGIN      
        1 aactgttgga ggtttaagca ctcgtcgcgc agtcatctat taaacgactc aatgaacgcg
       61 ttgtccaggg gtttacctgg tcgaaggcga agagctcttc cccaaggatt cgatttcgag
      121 acctgactca ccggttctct ccaccacatc cgaaatcggg cctctaacat gtcagccgtc
      181 cgccagccta tcatggaaac actccatact ccagcgcgtg ctaaagcagt cggaattggt
      241 gtcggctccc gagggagcgc ggcatgtttt cgacgggcga cccgcatgcg aagcggtaac
      301 ccatccaagc gtccaagctc gggatggcca tctgccatat tctggccaca cacagtaggg
      361 gcctttgctg atctgcgcac acgcgttact ttgtcggcag tatccacctg ccacaacgct
      421 gtcgcaatct gatccaccga aaccctcctt gagttctaag tccaagtgca acactgcaag
      481 ccctgatcgg ctacggtgtt gccatgataa agagatgtta cacgtatttg agtgacgcag
      541 aacgtgagac gttgagcctg ggcttggccc acggccattc gctgaagacg atggcggcgg
      601 tgctgggggc tcgccccaac accgtgagcc gcgagtctgc ccgcaacgcc gcgcggggcc
      661 gaccctatcg tgcctgcacg gcacagacgc tggcggcagc ccgggcgcgt caaccccggc
      721 ggtcgcgcaa actcctggcc ccctggctgt ggcagtacgt caggacacac ctggtccggg
      781 gctgctctcc cgaacagatt gccggacgcc ttcgacgcga gtatcctgac gacatgcaga
      841 aacgtctctc ggccgagacc atctatgtgg ggctgtatgt gctgccacgc ggcaccctga
      901 ggagcgcact gctggcggcg ctgcgtcagg cgcgcaaggc gcgccggcct cggtcgaggg
      961 gcacggatcg acgcggccac atcccgaaca tgacgccgat tgccgcccgc cctgcggaag
     1021 tggcgaaccg caccgtcccc ggccaatggg aaggtgacct catcaaggtg gcccgcaatg
     1081 gctcggccgt cggcaccctc gtggaacgga cgacgcgcct ggtcattctg gcccgcatgg
     1141 cgggcacgga tgcggggagg tgcctgcgag gggttcacaa ggaaactccg acacgtgccg
     1201 gcgccgctgc gcaagaccct ggcctacgat cggggcaaag agatggcgga acacgggcgt
     1261 ctggcccggc ggctcgccat tcaggtgttc tttgccgatc ctgacctagc cgggctgaat
     1321 tggtccagat gcaatgttag tctggacccc caggaggtca tcgcatgccc cgcaaaacgt
     1381 acacgcctga agaaatcatc caacacctgc gcacggtcga gctggaaact ggcaaaggga
     1441 ttgccgtcgt tgaggcttgc cgcaaggtcg gcatcacgga acaaacctac tatcggtgga
     1501 aaaaggagta cggcggcctg cgcgtggatc aagccaaacg gctcaaggcg cttgaacagg
     1561 agaatctgcg gctcaaacgg attgtggcgg atcaggccgt cgatctgtcg atcttgaagg
     1621 aagtcgcgtc gggaaacttc taagcccggc gcggcagcga gaggccgtgc gccacgcgat
     1681 ggcggggctg cacgtctccg agcgccgagc gtgccgggcg atcggccagc tgcgttccag
     1741 ctattgctat gtctcgcagc cggacccgac ggacgagcgg ttgtgggtac gggtcatcga
     1801 gctggcgaaa gaatacgggc ggtacggcta tcgaacgatc acgtggttgt tacaacggga
     1861 gggctgggac gtggggaagg atcgcgtgta tacgatttgg cggcacgaag gcttgaaggt
     1921 gccggcgaag cagccgaaac gcgcgcgact ctggcgggcc gacggctcgt gcgtgcgcct
     1981 gcggccgacc caccgccatc atgtctggtc gtacgacttt gtggcggagc ggacccatga
     2041 tgggcgtccg tttcgaatct tgaatgtgct cgatgagtga ccttcccccg tttgttacac
     2101 cacggccaac gaagttgact cctgcgctat ctggggctgg ttgtgatgat ggttgataaa
     2161 ctccctgggt gtcagattcc cgatggcact gtgcggccgc tcttcattgt actctcgtcg
     2221 ccacgcttca atcacaagct gggcttcctg tagcgtcaga aaccagtgct cgttcaaaca
     2281 ttcatcccga aacttgccgt tgaagctctc aataaacgcg ttctgcaccg gcttccctgg
     2341 ctgaatgaag tgcagatgca cgccgtgctg caccgcccag gcctccaagg ccgtcccggc
     2401 gaattcagga ccgttgtcca ggatcacggt gtccggcaac ggccgtgtgt tgaatagccg
     2461 gtcgagcacg cggcacaccc gcgccccgcc aatcgatacg tccacctcaa tcaccggcac
     2521 ctcttttgag tacagatcgg tcctcgtcaa acacttgaat cgccggcccc tgacgagccg
     2581 gtcatggaca acgtccatgg cataacaacg tcctggttgt gtgggcttgg acaaggcgac
     2641 tcgtggcacc gccgcagcct tcttccgtcg tcgccgccgt agtgacagcc cttcgtcacg
     2701 ataatagagc cgctccacct tcttgtgatt cacccgccag ccttcccgcc gtaaccggac
     2761 atagattcgg ggacacccat accgccgctt ggtttccgca atctctcgga tccgggtccg
     2821 cagcgcggca tcctcctggc gtcggctgcg ataccgcagc gtgttccgat cgagggccag
     2881 taagtggcac acccgtcgct gactcagccc aaagcgctcg gccatccact gcgccgccgt
     2941 tcgcttcgct ctgggcgcta ccagtttttt gcggtgacgg ccttcagcgc ctgaatgtcc
     3001 agcgcttgct ccgcgaccat ctgtttcagc cgacggtttt cgtcttcaag ctggcggagt
     3061 ttcttcacgt cgctgacttc cagcccggca tatttcgtcc gccacttata aaaggtcgcg
     3121 tccgagatcc cgtgcttacg acagagctcg tggaccccaa tgcccgcctg ggcatccttc
     3181 agtaccgcaa tgatctgttc ctccgtgtgc cgttttcgag tcatgggccc ctcctgtgga
     3241 ccccatctca gcacagtctg acttcctagc caatggtcta gttttcgggg gaaaggtcac
     3301 ttcacgtcgg aagcttccaa gccaccgtac ttggccttcc acttgtggta ggtcgcggag
     3361 ctgatgccgt acttcctcca gatctcgttg acggggcggc cggcatcggc ttctttcagg
     3421 atcgacacga tttgcgtctc ggtgaacttc gactgacgca tgggaacctc ctggctaggg
     3481 gggggctatt ctgccagaag attctcctgt tgcgtgtctc gatctacggg gagcttacag
     3541 ccagcctacc ctagttttga cctgccgtcg ttttccgagg agatgtcaag ggggcagcag
     3601 ctaaccgcag gatcaaaccg ttgtctggcc ttgtggtccg tgctacttgg cgtgtgtgcc
     3661 aggtccaggg ccttgtgcct gttcgcccaa ggcttggcgg ccgaatggaa gttggagaag
     3721 catctttgtg aggaatagag aggttatcag cgaaaaaaac gccaagcaga aaaagaagga
     3781 gagcgaggtt ggcggaagga tgcgcgagaa gatcaaggcc atccctttca tgaccagagg
     3841 aatatgcagg agatagatgc ccatcgtata ccggccaagc gtgctcagca ctcgaaggga
     3901 aaaggtggtc gaggcgaagg ctatgtagct tccaatcaac aacaggaact gcgcagggat
     3961 gaccgtatgg tctgatagga taggcaaaag gataccaccc gccagcgtga ccagggacag
     4021 agcgatgcca cgagttgcaa ggaaaccgga tacaagcggt atcaccaggc cgatcaaata
     4081 aaattgcagt ccccagagcg catggtagat gggatcgaga ccggggaaaa accatgtacg
     4141 aagcgggagg aggtggaacg cgccgagata cacgatcacg gcgagaaccc gaatgggcaa
     4201 gccgcattgg gcaaggatat gtagcggcgg cgccgcggca cgaataaaaa atagggatag
     4261 gagaaaatac atctgactgg agaagtcgga cagatagatc gcggtgatcc aatcttgcca
     4321 cgggcgcccg accaggatgt gggccggggc actgccggtg agttcgaaca tcagccggag
     4381 tacgccatag agcagagtga acagggccca agggatcagc agccggtgca tgcttttggc
     4441 gagatgctca cggtagtcga aagcggtctt ggcgccgtgg cggatgccga aaagtagccc
     4501 gtctacgaga aagaaaaccg gaacagcaat cgtttggacc atccaggcga tctggtactg
     4561 ctcatccccc gggaggtggg catatcccag cgcatgcgtg gcgacgacca tggcgatagc
     4621 cagaccacgg taattgtcga ggtgtacgag tctggtggaa ggcgaagctg gctgtgcagg
     4681 ggccatgagt tgtcttccaa cggtcaagct taaagggccc cgctcgtgtg tatgcccggg
     4741 ggccgctaga attgaaagta aatgaatgcc ggggcttcaa gtgtcacacc ggcatagaat
     4801 atcaatagta cggcatacgc ggcggcctga ataggaacgg gcctcgcgat ccaccactca
     4861 ccgaatcgtt cgagttgtgt aagtggggac aggtgggaga gtatgcctgc gcacaggatg
     4921 agataaaaga ctccgctgag cctggtccct cccgtgaatc gcgcgcagcc ctcgatgaag
     4981 tccataaagt tcttcatgtc ctggacgcga aagagtaacc aaccgaatgc gatgagctga
     5041 aaccagccta gtcgacgaag cacggtcccc aggagggtgc gcggcggacc agcttggctc
     5101 ctttgcggcg tcaacatcag cagcaatcca tggaatccgc cccagagcac gaagtgcatg
     5161 gcggctccat gccaaagccc tccaa
//
LOCUS       DAORQN010000103         5162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_653_length_5162_cov_24.593499, whole genome shotgun sequence.
ACCESSION   DAORQN010000103 DAORQN010000000
VERSION     DAORQN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_5162_cov_24.593499"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK614_06565"
     CDS             complement(<1..440)
                     /locus_tag="PK614_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRB81910.1"
                     /translation="MADIIHRVGIKAGPDNVFWALTTIEGLAAWWTENTTGVSDVGNT
                     VVFQFRDPNGEIKGGFEMDVLKQEPFKRVQWTCTKGPEEWVGTDITFDLKQEDDFTIV
                     LFAHRNWQESSEFTAHCSTKWAVFLMSLKQLIETGSGKPAPRDI"
     gene            complement(824..1921)
                     /locus_tag="PK614_06570"
     CDS             complement(824..1921)
                     /locus_tag="PK614_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRB81911.1"
                     /translation="MTKLASLAGVVAAILLTGLLTTFAIPVEQWRTGDQHLTELTYRQ
                     IPVPPNVPRRLWIDTDAACGYSRRTDPDDCFALALLAYASDFQLVGISTVFGNAPLDV
                     VNRTTIELAGKLSHEIGRTISVYSGSPGPLSQVATTPRPPAYEALTAALEQGPLTIVA
                     LGPLTNLAAVLSERPGLHSQDVRVVAVMGKRQGHLFHPAEGADTGILFGHGPIFRDFN
                     FVMDIDAAKQIVAMHLPLTLVPYDAARRIEMTADDLDHLSESSRALAWIADRADPWME
                     YWRHGIGRQGFYPFDLLAAAYIIDPSQFECASVDVRIGEDPTLFMPFGRSTALLVSEN
                     DRLSKETQEVGSARYCARPSSELKRRLMNHF"
     gene            complement(2028..3143)
                     /locus_tag="PK614_06575"
     CDS             complement(2028..3143)
                     /locus_tag="PK614_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB81912.1"
                     /translation="MTRTRGLQPIPFTHSRRQTASPAQTLFWCTLLLALSMLAASCAS
                     TQATGADASVPIQQSEYLAVDGAKLFLMTRGENRGAPILLWLHGGPGGAERPLFRYFN
                     SELERHFVVAYWDQRGAGRSFDSEADPHQLTVSRHLADLDTVVDHLRQTLGQDKVVIV
                     GHSWGSMLGLLYSQQHPEKVAAYIGVAQVVSWLKTQQGQYDFSLAESTNRKDEAALTH
                     LHNIGPPPYKTFDQQHKLYTLMNQYGGGFHKQPCKICVITKGMAIGLVTPWELISIHR
                     GFHATVDAMTPELLRFDLEQAVPRVEVPVSFFLGRYDRQVDSAIAARYFDTLQAPRKD
                     LVWFEASAHNIPFEEPDTFNRAVIEHAQAKTAGRLSK"
     gene            complement(3184..3792)
                     /locus_tag="PK614_06580"
     CDS             complement(3184..3792)
                     /locus_tag="PK614_06580"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRB81913.1"
                     /translation="MKKIDLKNELNHLYQPSAKQIVEVDVPSFQFLMIDGQGDPNTSP
                     EYTKAVEVLFSVAYTVKFLVKKGRQHIDYAVMPLEGLWWADDMSAFVANDRAQWKWTM
                     MIMHPSFVSEAVVKTAMAEVTRKKALPAVSKLRFETFAEGLCAQVLHIGPFSTEGPTI
                     AKVHQFIAARGKPSGKHHEIYLSDIRKTDPTKWKTIIRQPMR"
     gene            complement(3806..4231)
                     /locus_tag="PK614_06585"
     CDS             complement(3806..4231)
                     /locus_tag="PK614_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81914.1"
                     /translation="MPHARLGLLLTFTLIDGCGFADFARLSVNEPITIDNVAFIRPDQ
                     TTFAEVIARLGTPDEFLDSETGVFVVYRFLDARYSRINYGSLASAWSPVTPDLITEGL
                     GMRLNRLTIRLSPTWIVHHIEFSNQPEQPSGFTLVPFVH"
     gene            complement(4255..4674)
                     /locus_tag="PK614_06590"
     CDS             complement(4255..4674)
                     /locus_tag="PK614_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81915.1"
                     /translation="MSHPTTSPLIVTQGCLFRLLMVLVLASVAGCAFGRATLGDKIKQ
                     EDLASLTKGVTSRAEVVARIGAPDRILHANEREILQYYHYDLKSSSLLLLLVNFSRFN
                     IKSDDLYILIDREGLVDDVLYGRRTDKLAFQAWPFGE"
     gene            complement(4783..>5162)
                     /locus_tag="PK614_06595"
     CDS             complement(4783..>5162)
                     /locus_tag="PK614_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB81916.1"
                     /translation="ELLTHRLGTTLHRLDITPTPLDRALQLVQQNREVWRVDTLSELT
                     GCSARHIERAVMAHIGLPPKLLLRILRVQSAIAAIHHAPLRSLVDLAAMLGYSDQAHM
                     NRDFRLLTGNTPAQYQLALAQKA"
BASE COUNT         1112 a         1377 c         1447 g         1226 t
ORIGIN      
        1 ttgatatctc gcggagcagg tttaccgctc ccagtttcaa tgagttgctt gagactcatg
       61 agaaatactg cccactttgt actgcagtgg gcggtaaact cactggactc ctgccagttg
      121 cgatgggcaa acagcacaat cgtgaagtcg tcttcctgtt ttaaatcgaa ggtgatgtcc
      181 gtcccgaccc attcctcagg tcccttagta caggtccact gcacccgctt gaatggctct
      241 tgcttcaaca cgtccatctc gaagccacct ttgatctcgc cgttgggatc acgaaattgg
      301 aatacgacgg tattcccgac gtcggagaca cctgttgtgt tttctgtcca ccaagctgca
      361 agtccctcaa tcgttgtgag tgcccaaaac acgttgtcag gacctgcctt gattccgacg
      421 cgatgaatga tatcagccat gtcacacctc catatttagg taagcttgga ataatcagta
      481 attttgagaa agcgacaatc gtatttgcca atccggcagt tagccgttta cgaaagtcag
      541 gctaacaagc cgccttcaag cggctgatcc ttccgtagtt caagctcacg atgacgtgtt
      601 gtcgtcgcaa agagcgcttt cagccacgca ttccatgaca caacatggtg ctccatgtaa
      661 gactcctcct tttttttctg gctgcgattt ctgcgctgct gttggtcgaa tgcaaactcc
      721 aaaatcgact agtcgaaggt actgaaccgg agctctcaaa tatcaaggac cgtgggcggt
      781 tggcttggcc ctgttttcag tcctgaccga ttgctgctgc acttcaaaaa tgattcatga
      841 gccgtctctt gagctcgctt gacggccgtg cacaataccg ggcagatccg acctcttgag
      901 tctccttgga cagcctgtca ttctcactca ccagcaatgc cgttgaccgt ccaaacggca
      961 tgaacagagt tggatcctct ccgatccgga catcaactga ggcgcactca aactgactcg
     1021 ggtcgatgat gtatgcggct gcaaggagat caaacggata gaacccttgg cgaccgatgc
     1081 cgtgacgcca gtactccatc caggggtcgg cacgatccgc tatccatgcg agcgcacgac
     1141 tggattcgct caggtgatcg aggtcatctg cggtcatctc gatccgacgg gccgcatcgt
     1201 atggaaccag cgtcaggggt agatgcatag cgacgatctg ctttgcggca tctatgtcca
     1261 tcacgaagtt gaagtctcga aagatcggtc cgtgaccgaa gagtattccc gtgtcggcac
     1321 cttcagcggg gtggaacagg tgtccttgac gcttgcccat aaccgccacc acgcgaacgt
     1381 cctgtgagtg caggccaggt cgctcgctga gcacagccgc aagattcgtt agcggtccta
     1441 gggcgacaat tgtaagtggt ccttgttcga gcgccgccgt caacgcttcg tatgcaggcg
     1501 gccttggcgt cgttgcgacc tgtgatagag gtccagggct tccgctatag actgaaatgg
     1561 ttcggccgat ctcgtgcgac aacttccctg cgagctcgat tgtcgtccgg ttgaccacgt
     1621 cgagtggcgc gttgccaaac accgtggaaa tcccgacgag ctggaaatct gatgcgtatg
     1681 cgaggagtgc gagggcaaaa caatcatctg gatccgttcg gcgactgtat ccacaggcgg
     1741 catcagtatc aatccatagt cttcggggaa cattcggtgg cactggaatt tgccgatacg
     1801 ttagttctgt gaggtgctgg tcgccagtac gccactgctc gactggaatg gcaaacgtgg
     1861 tcagtaggcc tgtcaggagg atagccgcta caacgcctgc tagacttgcg agcttagtca
     1921 tccatttcac cattgaccgt gtcccattag aagctggccg cctgatgacc gcgtgccgtt
     1981 gctgacagca ccggggaggt gaggtatcgg catggctcgg gtggctctca cttgctcaat
     2041 cgccccgccg tcttggcctg ggcgtgttcg atcacggctc tgttgaatgt gtcgggctcc
     2101 tcaaacggga tgttatgggc tgatgcttca aaccagacca ggtcttttcg tggcgcctgt
     2161 aaggtgtcga aatagcgggc cgcgattgcg gagtccactt gccgatcgta tcgacctaga
     2221 aagaacgaga ccggaacctc cacgcgtggg acggcttgtt caagatcaaa cctcagtagc
     2281 tccggcgtca tcgcatctac cgttgcatgg aagcctcgat ggatggagat gagttcccat
     2341 ggcgtcacga ggcctatcgc catccctttg gtgatcacac agattttgca tggctgcttg
     2401 tgaaatcctc cgccatactg gttcatgagg gtatacagtt tgtgctgctg atcgaacgtt
     2461 ttgtagggtg ggggacctat gttgtgcaga tgtgtcaagg cagcctcgtc tttgcggttc
     2521 gtcgattccg ccaaagaaaa gtcgtattgt ccttgctgtg ttttgagcca tgacacgact
     2581 tgggccacac ctatgtacgc agcgactttt tcagggtgtt gctggctata gaggagcccg
     2641 agcattgatc cccaggaatg cccgactatt acaaccttgt cctggccgag ggtctgtctg
     2701 agatgatcca ccacggtatc tagatcggca aggtgtcgtg agacggtcaa ctgatggggg
     2761 tcggcctcgg agtcgaatga acgtcccgca ccccgttgat cccagtaggc cacgacgaaa
     2821 tgccgttcga gttcgctgtt gaaatagcgg aacaacggtc gctctgctcc acctggaccg
     2881 ccgtgcagcc acagtaagat cggtgcgccc cggttttcac ctcgtgtcat gagaaagagc
     2941 ttcgcaccgt ccactgcaag atactcggac tgttggatcg gaaccgatgc atcagctccc
     3001 gtggcctgag tgcttgcgca gcttgccgca agcatagaga gcgcgagtaa caacgtacac
     3061 cagaatagag tctgagccgg agacgctgtc tgccttctgc tgtgtgtgaa ggggataggc
     3121 tgtagtcctc ttgtccgtgt catggtcctc tccttcgacc gatcgtgatc gtgtgaagag
     3181 tcgtcatcgc atgggctgtc tgatgatggt tttccatttc gtcggatcgg ttttccgaat
     3241 atcgctcaag taaatttcgt gatgtttccc ggagggcttg cctcgagcag caatgaactg
     3301 atgcaccttt gcgatagtgg gcccttctgt ggaaaacggc ccgatgtgca gcacttgggc
     3361 gcacaagcct tctgcaaagg tctcgaatcg gagtttgctg actgccggta atgccttctt
     3421 cctggtcacc tctgccatgg ccgtcttgac caccgcttca gagacgaaag acgggtgcat
     3481 gatcatcatc gtccatttcc actgggctct gtcattggcg acgaaggcgg acatatcgtc
     3541 cgcccaccag agaccctcaa gcggcatgac cgcgtagtcg atgtgctgtc gtcctttctt
     3601 gacgagaaat ttcactgtgt acgccacgga gaacagcact tcaaccgcct tggtatattc
     3661 tggcgaggtg ttcgggtctc cttggccgtc aatcatgaga aactggaacg agggcacatc
     3721 aacctcaacg atttgcttgg ctgacggttg gtaaagatga ttcaattcgt tcttgagatc
     3781 gattttcttc atagctgctc tcccctcaat gaacaaacgg cacaagcgtg aatccagagg
     3841 gctgctctgg ttggttcgaa aactcaatgt gatggacgat ccaggtcgga gagagccgaa
     3901 tggtcagccg attcagccgc atccccaatc cctcggtaat caggtcaggc gtcactgggc
     3961 tccaggcact agccaggctc ccatagttga ttcgagagta tcgtgcatcg aggaatcgat
     4021 agacgacgaa gactcctgtc tcggagtcta ggaactcatc gggtgtgccg agcctggcaa
     4081 tcacctctgc gaaggtggtc tgatcaggac ggataaaggc gacgttgtcg atggtgatgg
     4141 gctcgttcac gctgagacgg gcgaaatccg cgaagccaca gccgtcgatc agggtgaacg
     4201 ttagcaatag accaaggcgt gcatgaggca tctgaatctc ccgctctaca aagctcattc
     4261 accaaagggc caggcctgga acgctaattt gtccgtccga cgaccataga gcacgtcgtc
     4321 gacgagtcct tcgcgatcaa tgagtatata gagatcgtcg ctcttgatat tgaaacgcga
     4381 aaagttgacc agcaacagga gaaggctgct tgatttcagg tcgtagtggt aatactgcaa
     4441 gatttctcgc tcgttcgcgt ggagaatgcg gtccggcgca ccgattcgag cgacgacttc
     4501 agctcgtgag gtcactccct tcgtgaggct tgcaagatct tcttgcttaa tcttgtctcc
     4561 cagcgtggct cgcccgaatg cgcagccggc gacggaggcg agcactagca ccatcagcag
     4621 tctgaacaga cagccttggg ttacgatcaa tggggatgtg gtgggatggc tcatgtctcg
     4681 ccctccttat gatcttcatg acaagatcag actagcgcag gctcgtgcta ggtatattgg
     4741 aaaaacccga catggcgggc tgaccacaaa gaccagttgc aattaggctt tttgggctag
     4801 cgcgagctgg tattgagcag gagtgtttcc ggtcagcaag cgaaagtcgc ggttcatatg
     4861 cgcttggtcg ctatagccga gcatggcggc cagatcgacc aagctcctca gcggggcatg
     4921 gtggatggct gcgattgcgg actgcacacg aaggatccga agcagaagtt ttggtggtag
     4981 accaatatga gccatcaccg cgcgctcgat gtggcgagcg ctgcacccag tcaactcaga
     5041 cagggtatcg actcgccaga cttcacggtt ctgttgcacc aattggagcg cccggtcgag
     5101 gggtgttggt gtgatatcta atcgatggag ggtggtgccc agcctgtggg tgagcaactc
     5161 tg
//
LOCUS       DAORQN010000104         5108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_656_length_5108_cov_27.322976, whole genome shotgun sequence.
ACCESSION   DAORQN010000104 DAORQN010000000
VERSION     DAORQN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_5108_cov_27.322976"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK614_06600"
     CDS             <1..624
                     /locus_tag="PK614_06600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRB81917.1"
                     /translation="RYLDAIQLSGTSQLVGTPLVLSPAIRERSRLVLQALIQENRRTV
                     VVIHPGSGSPHKCLDARLLVPVIEWLFQADMAPLLLEGPSDREAVERVLATIKIDVPV
                     IRGLALSTMAGVLSHAKLYVGHDSGVTHLAAALSVPMIACFGPTDSRRWAPLGRAVSI
                     VSGAPCTCRTWSAVAECRERVCLQIAPERIIEVCRLLLSERRTVPAT"
     gene            740..1987
                     /locus_tag="PK614_06605"
     CDS             740..1987
                     /locus_tag="PK614_06605"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HRB81918.1"
                     /translation="MSGSELRRVHVIRQVLAKQLTQHEAGETLGLTTRHIRRLRDRLR
                     AEGDDGLAHQGRGQPSNRRKSAAFKRQVLQLYGRHYADFGPTLAVEQLATHHGLTLSD
                     ETLRRWLRAQGIDHFRRRPRPHRTWRARRAHRGELVQLDGSHHDWLEGRGPRCVLMAY
                     IDDASSRVFARFYEYEGTLPALDSFQRYVKQYGIPLAVYADKHTTYRSPAEPTVEEQL
                     AGVAPMSQFGRALRDLGVELIPAHSPQAKGRIERLFHTLQDRLVKEMRLVGIATLAEA
                     NRFLPAWLTRYNRRFTVVPAHATDLHRPLPVGMDVKAVLALRTRRVLRRDWTVAHGGQ
                     LYQIQDRVRATHVLVEERVEGTLRLLHHGRPLTYQRLAQRPSALVRPTSPRRPRPWGK
                     PTPDHPWNRFGTFATARPREAIR"
     gene            2131..3885
                     /gene="argS"
                     /locus_tag="PK614_06610"
     CDS             2131..3885
                     /gene="argS"
                     /locus_tag="PK614_06610"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRB81919.1"
                     /translation="MTQGVVQDKVSQALVGALRSAQQRGVLKLEQMPMVNLEAPKRPE
                     WGDVSCTVAMSLSASERRPPFEIAQIIIDHIEQRDALFSRAEIARPGFLNLTVKRELW
                     MDVLREIEAQGERYGHSQVGQGRRVLVEYVSANPTGPLHVGHGRGAAVGQALVRLLCA
                     TGHDVVSEYYINDAGRQMKLLGLSVLARYREACGQTVSFPEDGYHGEYIRTVAEHVKA
                     EQGAALLSLPAADAEQRSREFAYRELLALIRQDLETFGIAFESWFSEASLLSSGAVEQ
                     VLSELRARDLLFDQEGAQWFRSSAFGDEKDRVVRKQEGDYTYLASDIAYHRDKLRRGF
                     DLLVDVWGADHHGYIPRMQAVVQAYGHPKERLRVVLVQMVNLLRGGQKVEMSKRSGEF
                     ITLREVMDEVGADAAKFFFLMRDSSTHLDFDLELAKQQSQENPVYYVQYVHARIASLQ
                     RVAASRGIACPLPSQADLNVLEDPDELALIRKLSAFPVVLQASAVELEPHRMAYYLRE
                     LAGMVHPFYNKHRILPPAPDSEAAAALSTEQSGSSGRATEVMTPGLTGARLALMWAVQ
                     QVVRNGLNVLGVSAPEQM"
     gene            3899..5056
                     /gene="tgt"
                     /locus_tag="PK614_06615"
     CDS             3899..5056
                     /gene="tgt"
                     /locus_tag="PK614_06615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRB81920.1"
                     /translation="MLTFRITHEESGGRARVGVLTTARSEIATPAFMPVGSLGNVRSV
                     DGEELLKMGYGLILNNAYHLYLRPGHKVVEELGGVHGFTAWPGAILTDSGGFQVFSLA
                     KFCKISDEGVMFQSHIDGSSRFISPETSIEIQEALGADIIMAFDHVVALPSSLDQVRD
                     ASKRTSLWARRCVSAKRRTDQSLFGIVQGGLDAGLRVESARDLVSVGFDGYAVGGLSV
                     GEDKADMYGMLDVTVPELPSAKPRYLMGVGMPEDLVEGVARGIDMFDCVVPSRHGRTG
                     WLFTSFGRVVIKQARYARDEQPIDPACRCPVCTRYTRAYLHHLFGVKEMLGARLNTIH
                     NLWFFAKLMEEIRSAVRGGTFQEFRREFHRTYVVDRPADDGRSDIQSTNGS"
BASE COUNT          911 a         1376 c         1699 g         1122 t
ORIGIN      
        1 cgatatcttg atgccattca actgagcggg actagtcagc tagtcggtac tccactcgtt
       61 ctctctccgg ctattcgcga acgcagcagg ctcgttcttc aggcactcat tcaagagaat
      121 cggcgcacgg tggtggtgat tcatccgggg agcgggagcc cccataagtg tttagatgca
      181 cggcttctcg tgcccgtgat tgagtggttg ttccaggccg acatggctcc cctattgctg
      241 gaaggcccgt ctgatcgtga ggctgtggag cgcgtcctcg cgactataaa gatcgacgtg
      301 ccggtcatcc gtgggctggc tttgtccaca atggcagggg tgctgtcgca tgcgaaactc
      361 tatgtgggac acgattctgg cgtgacgcac ctcgccgctg ccttgtcggt tcctatgatc
      421 gcttgttttg gtcctactga ttcacgtcgt tgggctccgc ttggtcgcgc cgtgtcgatt
      481 gtatctggag cgccctgtac ctgccggact tggagtgccg tggcagagtg ccgtgagcgc
      541 gtgtgtcttc agatcgctcc ggagcgcatc attgaggtct gccgactgtt attgtcagag
      601 cgacggacgg ttcctgccac ctaactcgcg atctgtaatc ccacaatgat aatgtccgct
      661 tctcccacag tagaaatgtc ctctatagac tgccgactct cacgaggagg gcaggatggt
      721 cggagaggac agaatcgtca tgagtgggag cgaactgcgg cgggtccatg tgattcgcca
      781 agtccttgcg aagcaactca cacagcacga ggccggtgag acgttagggc tcacgacgcg
      841 gcacattcga cggctccgcg atcggttgcg ggccgaaggc gacgacgggc ttgcacacca
      901 gggacggggc cagccgtcga accggcggaa atcggccgca ttcaagcgtc aagtgttgca
      961 gctctatggc cggcactatg cggattttgg gccgacgttg gcggtggaac agctggcgac
     1021 gcatcatggc ctcacgctca gtgatgagac cctgcggcgc tggctacgag cccagggcat
     1081 cgatcatttt cggcgccggc cccgtccgca tcggacctgg cgagcgcgac gcgcgcaccg
     1141 cggcgaactg gtgcagttgg atggctccca tcatgattgg ttggaggggc ggggcccgcg
     1201 ctgtgtgctg atggcctaca ttgacgatgc gagcagccgg gtgttcgccc gtttctacga
     1261 gtatgaaggg acgctgccgg cgctcgacag cttccagcgc tacgtgaagc agtatgggat
     1321 tcccctggcc gtgtacgccg acaagcatac gacctatcgc tcccccgcgg aaccgacggt
     1381 ggaagaacaa ttagccggcg tggcgccgat gagccagttt ggacgggcgc tccgggatct
     1441 gggcgtcgag ctgatcccgg cccactcgcc ccaggccaag ggccgcatcg agcggctctt
     1501 tcacaccctg caagatcggt tggtcaagga gatgcgcttg gtcggcatcg ccaccctggc
     1561 ggaggcgaat cggttcttgc ccgcgtggct gactcggtac aatcggcggt ttacggtcgt
     1621 gccggcgcac gccaccgacc tgcatcgccc cctcccggtt ggcatggatg tgaaggccgt
     1681 attggccctg cggacccggc gggtcctacg gcgggattgg accgtggccc acggcggcca
     1741 gctctaccaa atccaggacc gtgtgcgggc gactcacgtc ctcgtggaag agcgggtgga
     1801 gggcacactc cgcctgctcc atcacggtcg cccgttgacc tatcagcgcc tcgcgcaacg
     1861 gccttccgcc ctcgtgcgtc cgacgtcccc tcgacgacca cggccttggg gcaagccgac
     1921 gcctgatcat ccgtggaatc ggttcgggac attcgcgacc gcccggcccc gcgaggcgat
     1981 tcgatgaacc ggacattcct actgtggcag gaagcggaca tttctaaagt gggtggacac
     2041 acctaactcg cgatcttgtc tctcatgtca tggtgtgtta gattcccagt ttgattttcg
     2101 ctgtttccac gcagacttag aggggttttt gtgactcagg gtgtggtgca agacaaggtt
     2161 agtcaggcgt tggtcggggc gcttcgatcg gcgcagcaac gaggggtgct caagctggag
     2221 cagatgccga tggtcaacct cgaagccccg aagcggcccg agtggggtga tgtgtcgtgc
     2281 accgtcgcca tgtctttatc tgcatcggag cgccgccctc cgtttgaaat cgcccaaatt
     2341 attatcgatc acatcgagca gcgggatgca ttgttcagtc gtgcggaaat cgcacgaccg
     2401 ggctttttga atctgacggt caagcgagag ctctggatgg atgttctgcg cgagattgaa
     2461 gcgcagggcg agcggtatgg ccacagccaa gtggggcagg gacgacgagt gctcgtggag
     2521 tatgtcagcg ccaatccgac cgggccgttg catgtcgggc atggccgcgg tgctgctgtg
     2581 gggcaagcgc tagtccgtct cttatgcgct accggacatg atgtcgtcag tgagtactac
     2641 attaacgatg ctggccggca gatgaagttg ctaggtctgt cggttctggc gcggtaccgg
     2701 gaggcttgtg ggcagacggt gtcctttcct gaagacgggt atcatggcga gtacattcgc
     2761 acagtcgccg agcatgtgaa ggcggaacag ggcgcggcgc tgctctcgct tcccgccgct
     2821 gatgcggagc aacggagtag ggagttcgcg tatcgcgaat tactggcact cattcgtcag
     2881 gatctggaga cgttcgggat cgcctttgag tcctggttca gtgaagcctc gttgttgtcg
     2941 tccggcgccg tggagcaggt actgagcgag ctgagagcca gggacctctt gttcgatcag
     3001 gagggtgcgc aatggtttcg atcgtcggcg ttcggtgatg aaaaggatcg ggtggtccgc
     3061 aagcaggagg gggactatac ctatctggct tccgacatcg cctaccatcg agataagttg
     3121 cgacggggct ttgatctcct ggtcgatgtg tggggcgccg accatcatgg gtatattccg
     3181 aggatgcagg cggtagtgca ggcctatggc cacccgaagg agcgactgag ggtcgtgctg
     3241 gtgcagatgg tgaatctgct tcgtggaggc cagaaggtcg agatgtccaa gcggtccgga
     3301 gagttcatca cgctgcgtga ggtgatggac gaggtcgggg cggatgccgc caaatttttc
     3361 tttctcatgc gcgattccag tacgcatctg gatttcgatt tggaattagc caaacagcaa
     3421 tcgcaagaga acccggtcta ctacgtgcaa tatgtgcatg cgcggattgc cagtctccag
     3481 cgtgtggcgg cttcgcgggg cattgcttgt ccccttccga gtcaggccga tctcaatgta
     3541 ttggaagatc ctgatgaact cgcgctcatt cggaaactgt cggcgttccc ggtggtgttg
     3601 caggcgagtg ccgtggagtt agagccgcat cgaatggcct actatcttcg tgagttggcc
     3661 ggcatggtgc acccctttta taacaagcac cggattttac ctccggcacc agacagcgag
     3721 gcggcagccg ccttatccac tgagcagtcc ggctcctcag gccgggcaac tgaggtgatg
     3781 acgccgggcc tgacgggtgc ccggctggca ctgatgtggg ccgtgcagca ggtggtccga
     3841 aacgggttga atgtgttggg cgtgtcagcc ccggagcaga tgtaggactg ccgatcccat
     3901 gttaaccttc cgcatcaccc atgaagaatc cggcggacgc gcgcgcgtcg gcgtgttgac
     3961 caccgcccgc agtgagatcg cgacaccggc gtttatgccg gtcggctcgc tgggcaatgt
     4021 gcgcagtgtc gatggagagg aattgctcaa gatggggtat ggactcatct tgaataacgc
     4081 ctatcacctg taccttcgtc ccggacataa agtggtggag gagttgggcg gggtgcatgg
     4141 gtttacggca tggcccggcg ctattctcac cgatagcggt gggttccagg tctttagttt
     4201 ggcaaagttt tgcaagattt ccgatgaagg cgtgatgttt caatcccata tcgacgggtc
     4261 gtcgcggttt atcagtccgg aaacctccat tgaaattcag gaagccttag gcgcggacat
     4321 catcatggcg ttcgatcatg tcgtggcatt gccgtcctcc ctggatcagg ttcgtgatgc
     4381 ctccaagcgg acgtcgctgt gggcgcgacg gtgcgtcagt gccaaacggc ggacggatca
     4441 atcgttgttc gggatcgttc agggcggcct ggatgccggc ttgcgggtgg agtcggcgcg
     4501 tgatctggtc tcggtggggt ttgacggata tgccgtcggt gggttgtcgg tgggcgaaga
     4561 caaagccgac atgtatggca tgttggatgt cacggtgccc gagttgccgt cggccaagcc
     4621 tcgttatttg atgggcgtgg gcatgcccga ggatctggtt gaaggggtgg cgcgcgggat
     4681 cgatatgttc gattgcgtcg tgccgtcgcg tcacggccgc acaggttggt tgtttacctc
     4741 gttcgggcgt gtggtgatca agcaggctcg gtatgcgcgt gatgagcagc ctatcgatcc
     4801 ggcttgccgg tgtccggtgt gcacgcggta cacccgggcg tacttgcacc acttgttcgg
     4861 cgtaaaagaa atgttgggcg cccggctgaa tacgattcac aacttatggt ttttcgcgaa
     4921 gttgatggag gagatccgga gcgccgtgcg aggcggaacc tttcaggagt ttcgccgtga
     4981 gtttcatcgg acgtatgttg tggatcggcc ggcagatgat ggtcgatcgg acattcagag
     5041 tacgaacggt tcttaacggc ggcacaggcg ggggacatta tgtgggattc ggtggcatgg
     5101 gcgcaggg
//
LOCUS       DAORQN010000105         5049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_662_length_5049_cov_8.183020, whole genome shotgun sequence.
ACCESSION   DAORQN010000105 DAORQN010000000
VERSION     DAORQN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_5049_cov_8.183020"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2137)
                     /locus_tag="PK614_06620"
     CDS             complement(113..2137)
                     /locus_tag="PK614_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRB81921.1"
                     /translation="MSDYAVLGDLLVIYAVSTAVVFVFHQFRLPSIAGFLVAGALIGP
                     HGLHLIPDVSQVQVLAEIGIVLLLFTIGMEFSSAHFAAARRILMVAAPVQTGGVLVLA
                     LLGALAVGLSYQQGIFWGFLLSLSSTAIVLKALSEQGESDSFHGRATVAILIFQDLAV
                     VPMMLVTPILGTPSGSAMGTVLMTLVKAAIVVGLIVAAAWYLVPRLLRHIVRSRSREL
                     FLLSIIVLCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQALAEVLPFRDSFNSLF
                     FVSIGVLMDMRVVLEHPFIVLGLLLAVLVGKLITGSGAMVAAGAPPRSSVLVGVALAQ
                     VGEFSFILAQQGQDAGLFTGDQYQLFLAVSVLTMVVTPFLMQWSPDLGRRIEAMQRVR
                     GWMPTRTAAHVEQLEGSQIRIKDHVIIVGYGLNGRNLARVLSETEIPHVALDLDGETV
                     RREARHGVPIYYGDGSNANVLRHMKIDDAKVLVVALSDPFTARRTVKVAKGLNPKLHI
                     VVRTRYLRELEELHQLGADDVVPEEFETSIEIFALVLRTYSLPQEFVARKAEQIRREG
                     YALLRRSEMPELAHHLRGGTLTDVEVETCRIDDEAPAVGKSLTELSIRPRTGASVIAW
                     TRSQVTQSNPSETVRLQAGDVVTLLGSRDQIRRAMALLTEPNHGTSHQLK"
     gene            2315..2986
                     /locus_tag="PK614_06625"
     CDS             2315..2986
                     /locus_tag="PK614_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRB81922.1"
                     /translation="MFDFNGVIADDETPHLHCFQQALAEVGLSLSKEEYYGTYLGMDE
                     RTCATTLLERRDGSCDPAIHAGIIERKASLFRAHTALHKPALFPWVTEFVQRASGAYR
                     LAVASGGRREQILAALTGTPIEQAFELIVPADDCAVGKPDPAIYEFTLRQLNARRPRP
                     PLLRASECLVIEDSRAGVLAGLKAGMRVLAIATTYPASQLTEAHLVLSTLDGVDPADL
                     ARRLF"
     gene            complement(3043..3429)
                     /locus_tag="PK614_06630"
     CDS             complement(3043..3429)
                     /locus_tag="PK614_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81923.1"
                     /translation="MIWSLLAAGWLMLAGQGLALAEAPSTEIATWSELRSQSSNVLSQ
                     APATSERQVDIFRQTPMLSGRVQVSDQTLIPYVGAGFGGGYVTERDRALGPMPGLPQQ
                     NLLGDSLGLGKGMMPNEFQMGIRIPF"
     gene            complement(3866..>5049)
                     /locus_tag="PK614_06635"
     CDS             complement(3866..>5049)
                     /locus_tag="PK614_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB81924.1"
                     /translation="FSSLFMGEGWMPLVPTELPDVYATLWEESGVKLWTVVNRADRSM
                     SGVILNVPAVKGGQYFDLVEGRELFPRINGDMVTLEGAIARRGIAGFVAGNRTALGMD
                     FYAFLKARQSSHSEIDERMDVPHRPVNLLGRKESPDYSIVRVPSGMVVIPAASFTIRV
                     KLRVRECGFYGNEISREELYANFHKLRMFERAVSLNRFAIDLTPVTNAQYAEFLHDSG
                     YQPQHRKNFLKHWTNRTPPVGMEEHPVVYVGLEDARAYAAWAGKQLPTEEEWQYAAQG
                     ASGLKYPWGEVMEPRRCNAGETGATTNVTAFPDGRSPFGCYDMCGNTWEWTESERTDG
                     RTRFCMIRGGSYYTAKGSHWYMDGGPQPSDFAAKFLLMWPGLDRCATIGFRCVVPVEK
                     L"
BASE COUNT         1153 a         1595 c         1357 g          944 t
ORIGIN      
        1 cgaggtcgat ccgcccggcg actccgaaat ggtccggggt gccgcgtccg cctttcccaa
       61 cactccgctg aaagaccgcg tgacgcttcg ctggagaagt tagccgaccg cgctatttga
      121 gttggtgact cgtgccatga ttgggctcag tcaagagcgc catggcccga cgaatctggt
      181 cgcgggatcc gagcagcgtg acgacatcac cggcctgcag ccgcacggtt tcagatggat
      241 tggactgagt gacctggctg cgagtccagg caatcaccga cgcgccggtc cgtgggcgaa
      301 tggagagttc cgtcagcgac tttcccaccg ccggagcctc gtcatcgatc cggcaggttt
      361 ccacctcgac atcggtcaac gtgcctccgc ggagatgatg ggccaactcc ggcatctcgc
      421 tccgacgcaa cagcgcatac ccttcccgcc tgatttgctc cgcctttcgt gccacaaatt
      481 cctgcggcaa actataggtg cgcagcacca gggcaaagat ttcaatcgag gtctcgaact
      541 cttccggcac cacatcgtcc gcacccaact gatgcaactc ttccaactct ctcaaatagc
      601 gcgtccgcac gacgatgtgg agcttcggat tcaatccttt ggccaccttc accgttctcc
      661 tggccgtgaa cggatcggac aaggccacga ccagcacttt ggcgtcgtcg atcttcatat
      721 gccgaagcac gttggcattc gagccgtcac cgtagtagat cggcacgcca tgacgcgcct
      781 cgcggcgcac cgtttcgccg tccaggtcca gggccacatg cgggatttcg gtttcgctca
      841 atacgcgggc gaggtttcga ccgttgagcc cgtagcccac aataatgaca tggtctttga
      901 tccggatctg agaaccttcc agctgctcga catgcgcggc ggtgcgggtc ggcatccagc
      961 cccggacgcg ctgcatcgct tcgatccgcc gccccagatc gggcgaccat tgcatcaaaa
     1021 acggcgtcac caccatggtc aacaccgaca ccgccagaaa taactgatac tgatcgccgg
     1081 tgaacaaacc ggcatcctgc ccctgttgcg ccaggataaa actgaattcg ccgacctgcg
     1141 cgagcgccac cccgaccaac accgacgatc tgggcggagc cccggccgcc accatcgccc
     1201 cgctcccggt gatcagtttt ccgacaagga ccgccagcag gaggcccagc acaatgaagg
     1261 ggtgctccag caccacccgc atgtccatca agacaccgat cgacacgaaa aacaggctat
     1321 tgaaactgtc acggaagggc aggacctcgg ccagcgcctg atggctgtac tcggattcag
     1381 aaatgaccaa cccggcgata aacgcaccga gcgcgagcga aagtccgccc agggacgtca
     1441 gccaggcaat cccgagacag aggacgatga tcgagagtag aaatagctcc cggcttcgac
     1501 tccgcacgat atggcgtagg agccgcggca cgaggtacca ggccgccgcg acgatcaacc
     1561 cgaccacaat cgccgccttg accaaggtca tcagtaccgt ccccatcgca ctcccgctcg
     1621 gagtgcccag gatcggcgtg acgagcatca tcggaacgac agcaaggtcc tggaagatca
     1681 gaatcgcaac ggtggcgcga ccgtgaaacg aatcactctc accctgttcc gaaagggctt
     1741 tcagcacgat ggccgtgctg cttaatgaga ggagaaagcc ccagaaaatc ccttgttgat
     1801 acgagaggcc caccgccaaa gccccgagca acgccaggac aaggacgccc ccggtttgaa
     1861 ccggcgcggc aaccatgaga atgcgccggg cagccgcaaa gtgcgcggag gaaaattcca
     1921 tcccgatcgt aaagagcagc aacacgatcc cgatctcagc caggacctgc acctgcgaca
     1981 catcgggaat cagatggagc ccgtgcggcc cgatcagggc tccggccacc aggaacccgg
     2041 caatcgatgg aagacgaaac tgatgaaata caaagaccac tgcagtggac actgcataga
     2101 tcaccaagag atcgccgaga actgcgtagt cgctcatcgt gtcatgggag tcgatttgtg
     2161 gtggcgaacg gtccggatac gttgccgtga cgcagcatac cgtagggacc tcagcgggac
     2221 aaggagttcc gcttgtccga ccacccgcgc tcggttagag tagcgcgcac cacgtaggac
     2281 aatgtgagcc ggggaggccg ctctgcgcgc catcatgttc gacttcaacg gcgtcatcgc
     2341 cgacgacgaa acgccgcacc tgcactgctt tcagcaggcg ttggcggaag tgggtctcag
     2401 cctgagcaag gaggaatact acggcaccta cctgggtatg gatgaacgaa cctgcgcgac
     2461 cacattattg gaacgacgcg acgggagctg cgacccggcg attcacgccg ggattatcga
     2521 acgaaaggca agcttgtttc gcgcacatac ggctctgcac aaacccgcat tgtttccctg
     2581 ggtcaccgag ttcgtgcagc gcgcatcggg agcgtacagg ctggcagtgg cgtccggagg
     2641 cagacgagaa cagatccttg ccgcactcac cggcaccccc atcgaacagg catttgagtt
     2701 gatcgtgcct gcagatgact gcgccgtcgg gaaaccggac cctgcgatct atgagttcac
     2761 cctcagacag ctgaacgccc gtaggcctcg tccaccgttg ctcagggcga gcgagtgtct
     2821 ggtgatcgaa gattctcggg cgggagttct ggcaggcctg aaggcgggca tgcgcgtcct
     2881 cgccattgcg acgacctacc cggcatccca actaacggag gcgcacctgg tcctttcaac
     2941 gctggatgga gtcgacccgg cagacctcgc ccggcggctc ttttagccac tggaccagaa
     3001 gggatgattc atgctgtggt gcagcggcgc aaccggcaag acttaaaacg gaatgcgaat
     3061 gcccatctga aattcgttcg gcatcatgcc cttgcccaag cctaacgagt cgcccaagag
     3121 gttttgctgc gggagtcccg gcatgggacc gagggcccga tcccgctccg tcacgtatcc
     3181 cccgccgaat ccggccccca cataagggat cagagtctgg tcactgacct gcacgcgacc
     3241 actcaacatg ggcgtctgcc gaaagatgtc gacttgcctc tcactcgttg ccggagcctg
     3301 ggacaagacg ttcgatgatt ggctacgcaa ctcactccaa gtcgctatct cggtcgaagg
     3361 cgcttctgcc agcgcaagac cctgccccgc cagcatcagc catcctgctg cgagcaacga
     3421 ccaaatcatt tgatttcttt tcacgtccac tgaagccctt tgcataaagg gggagactct
     3481 atttttaatt tactccatac tcccagctgc aacaagtacc gactcagcat cccagacaag
     3541 tcggctcgca acgactcgag acgccgcgcc acgcgactca tcttaatttg tcgggacggt
     3601 ttgactgcgc ttaccgacag cagaatggaa ccgcgtcgca gaactgcaag gacgaaggac
     3661 gatccggcac tggcggggaa ccctggaaca tcacgttcac acgatcaagc aattcgggac
     3721 cattgcaccc gtcgtcgcac atcccctctg cggggatatc accgaacgct tgcacgggtt
     3781 cgagcaactc tgccggttct tatcgcaata cgaaaccagg tgggcgtcgg aggtgattca
     3841 cccggggggc tagactacac atgcgttaca acttctcaac cgggactaca caccgaaacc
     3901 caatggtcgc acatcgatcc agacccggcc acatgagcag aaacttcgcc gcaaaatctg
     3961 acggttgagg gcctccatcc atataccaat gagagccttt cgccgtatag tatgaaccgc
     4021 cgcgtatcat acagaaccgt gtgcgcccat ccgtccgttc actttcggtc cactcccacg
     4081 tattgccgca catatcatag catccgaacg gagatcgacc atcagggaac gccgtgacat
     4141 ttgtagtcgc accggtttcg ccagcgttac atcgtctcgg ctccatcacc tctccccaag
     4201 gatattttaa tcccgaagcg ccctgcgcag catattgcca ttcttcctcg gttgggagtt
     4261 gcttgccagc ccaggccgca taggctcttg catcctccaa gccaacatac accactgggt
     4321 gctcctccat gccgactggt ggggttcgat tggtccagtg ctttagaaag ttcttcctat
     4381 gctggggttg gtaacctgaa tcgtgcaaga attctgcata ctgtgcgttc gtcacgggcg
     4441 tcaggtcgat tgcaaaccta tttaatgaga cagcacgctc gaacatgcga agtttatgaa
     4501 agttggcata aagctcctcg cgggatattt cgttcccata gaagccgcac tctcgaactc
     4561 gcaatttcac acgaatcgtg aaggacgcgg ctggaatcac caccatccca gagggaaccc
     4621 tgacaattga atagtcaggg ctctcttttc gacccagcag attaaccgga cgatgaggta
     4681 cgtccattct ctcatctatc tctgaatggc tggattgtct cgccttaaga aaagcataaa
     4741 agtccatgcc cagcgcagtc cgattccctg ccacaaaccc agcgatccca cgtcgagcaa
     4801 tggccccttc gagggtgacc atatctccgt taatccgagg gaataattcg cgtccctcaa
     4861 ctaaatcaaa atactgtcca cccttcacag cgggaacatt caggatcact ccggacatcg
     4921 aacgatctgc ccggttcacg acggtccaga gtttgacgcc agattcttcc caaagggtcg
     4981 cgtatacgtc cgggagttcc gtggggacga ggggcatcca gccctctccc ataaaaaggg
     5041 atgaaaatc
//
LOCUS       DAORQN010000106         4921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_672_length_4921_cov_531.765927, whole genome shotgun sequence.
ACCESSION   DAORQN010000106 DAORQN010000000
VERSION     DAORQN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_4921_cov_531.765927"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PK614_06640"
     CDS             complement(<1..148)
                     /locus_tag="PK614_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRB81925.1"
                     /translation="MAALNLVVISGLSGSGKSHALKAFEDAGYFCVDNLPPALLPTFV
                     ELCHQ"
     gene            complement(135..329)
                     /locus_tag="PK614_06645"
     CDS             complement(135..329)
                     /locus_tag="PK614_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81926.1"
                     /translation="MVRPTLEVLTVEEAVEALNSAKLAVLVFVNALSGTIQVLQRMPN
                     GRVSLIDPASDRARTAHGRA"
     gene            complement(337..687)
                     /gene="raiA"
                     /locus_tag="PK614_06650"
     CDS             complement(337..687)
                     /gene="raiA"
                     /locus_tag="PK614_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRB81927.1"
                     /translation="MRLMITGRHVAVTPALREYIETRMERLDRYGLKLGTLQILLSVE
                     KFHHVAEVVGVVQSRRLQAKTSTEEMYASIDEVVDKLGAQLRKLKERKVNHKFGDQPR
                     VRGSCTEGSTGCAQ"
     gene            complement(794..2311)
                     /gene="rpoN"
                     /locus_tag="PK614_06655"
     CDS             complement(794..2311)
                     /gene="rpoN"
                     /locus_tag="PK614_06655"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRB81928.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLQQHLMEN
                     PLLDELATETEESEETATAEREQPDTSTTVSSEVRGEGDDKPAESGENAEEFSASSWE
                     DYFDTDMRRGETEYSSSSKEEFPSYEQTVAKSTSLEDHLVWQLSLSGLSDREKAIGRL
                     IIGNLDDDGYLRMSLDELVAGTDYSVAEAESVLKDVQGFDPNGVAARDLSECLLLQLK
                     FLGRSQIGSLGSRPGVLKGTVLESIVLHHLKDLEKKQYNRIAKALNISMDDVFEATRI
                     IEGLEPKPGRPFSNTQNYAIVPDVFVVKNEGVWEVLLNDDGLPRMRISPYYKQLMSSG
                     QSGSAETKAYLDDKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEGFFERGIQHLKPLV
                     LKQVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPTDMLSSLTVRE
                     MIRQMVAAEDARKPLKDEEIAARLRTQQVLIARRTVAKYRAEDNIPSATQRRKFF"
     gene            complement(2332..3057)
                     /gene="lptB"
                     /locus_tag="PK614_06660"
     CDS             complement(2332..3057)
                     /gene="lptB"
                     /locus_tag="PK614_06660"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRB81929.1"
                     /translation="MPDRLAANGLVKSFRGRKVVKGVSLDVQAGEVVGLLGPNGAGKT
                     TIFDMMVGLCQPDEGTIALSGQEITDLPMYKRARRGIGYLPQESSVFRRLSVEHNILA
                     ILEMLGYSRSERMERVETLLKELDLVHIRKSKAYALSGGERRRLEITRALATSPSFML
                     LDEPFAGIDPIAVADIQQIIIRLKQRNIGVLITDHNVRETLSITDRAYIINEGTILEA
                     GPPGVIEKSEMARAVYLGEGFRL"
     gene            complement(3114..3707)
                     /locus_tag="PK614_06665"
     CDS             complement(3114..3707)
                     /locus_tag="PK614_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRB81930.1"
                     /translation="MWCLLLSGIALQTLPWAHGAEPSVAKESTSNPAPTTITSRTMTV
                     SNQANTAIFDGAVVLTRGLLVVHSDHMVVSFHPNRNGADSKNANGATKPKNNSSANKP
                     EPQGQDAAPAVSERSIRMVEATGRVRIEKEDGRATCQKAVYYEDEKKIILTGDPVAWQ
                     KGTRVSGKRIIMFLDEDRSVVEGDSQVVIEGESGGKR"
     gene            complement(3688..4260)
                     /gene="lptC"
                     /locus_tag="PK614_06670"
     CDS             complement(3688..4260)
                     /gene="lptC"
                     /locus_tag="PK614_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRB81931.1"
                     /translation="MWERWIRRGLLALSVVLAAFLGYLLVTRSNPGSSSRSVAPVDTE
                     SADARIQDFTFTQTKGDLVQWKVQAEQARLFEKDSRAILNNVQITMYGAQGKELTLSG
                     DEGTLDTQTKNFQLSNRTTPIVVETQSGYTIQTNHLVWTDARHEIQTPDHVTINGHGL
                     QVTGRGLLGKMDTEEFQVLDDVRVDVVPAS"
     gene            complement(4449..4895)
                     /gene="rplQ"
                     /locus_tag="PK614_06675"
     CDS             complement(4449..4895)
                     /gene="rplQ"
                     /locus_tag="PK614_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRB81932.1"
                     /translation="MRHKKKGRQLGRQTKHRWALFRSLVTSLLEHERIETTEAKAKEV
                     RGFTDRMITLGKEGGLPARRRALSFLRSKAVVAKLFSDVASRFRERPGGYTRIIRTRR
                     RIGDAAEMVAIELVTRPEKPKAVPSADVAVAEKKDTTEKTSAQAES"
BASE COUNT         1017 a         1540 c         1218 g         1146 t
ORIGIN      
        1 gctgatggca taactcgaca aaggtgggaa gaagggccgg cggaagattg tcgacgcaga
       61 aataccccgc gtcttcgaag gctttcaagg catggctctt tccggatccg gaaaggccgc
      121 tgatcacgac gagattaagc gcggccatga gcagtgcgag cacggtcgct cgcgggatcg
      181 atcagagaca ctcgtccatt cggcatgcgc tggagaacct gaatcgtgcc ggacagcgca
      241 ttcacaaaga ccagcaccgc gagcttcgca gaattgagcg cctcgacagc ctcttccacc
      301 gtcaacactt cgagcgtcgg tcgaaccact tcgacctcat tgcgcgcagc ccgtggagcc
      361 ttccgtgcaa ctgccccgca cacgtggttg atcgccgaat ttgtggttaa ccttgcgctc
      421 tttcaatttc cgcaactggg cgccgagttt atcgacgact tcatcgatcg aggcatacat
      481 ttcctcggtc gacgtcttcg cttgcaaacg ccggctctgc acgacaccca ccacttcagc
      541 cacatgatga aacttttcca cgctgagcag aatctgcaag gtcccgagct tcagtccgta
      601 gcggtccaac cgttccatgc gtgtttcgat atactcccgt agagcaggag tgactgccac
      661 atgccgaccc gtgatcatca atcgcattcc atcctcgttt ctcaggctgc ccgcaccacc
      721 gtacgttcat gcccgttgcg acgattgttg tacccccagg ggaacttcct ccctggtgcc
      781 agacccgacc agcttagaaa aacttcctgc gttgggtggc ggagggaatg ttgtcctccg
      841 cacgatactt cgcgacggtg cgacgggcga tcagcacttg ctgcgtccgc agccgcgcag
      901 cgatctcctc atctttgaga ggcttgcgcg catcttccgc cgcgaccatt tgccggatca
      961 tttcgcgcac cgtgagggac gacagcatgt ccgtcggctg atccgcgcgc tgaagcccgg
     1021 cgttaaagaa aaatttcaac tccaacatcc cctgcggaca atacatatat ttattggccg
     1081 tcacgcggct gatcgtcgac tcgtgcatcc cgatatcctc ggcgacttgc ttgagaacca
     1141 acggtttcaa gtgctggatc ccgcgctcga aaaaaccttc ttgaaacttc acgatgctcg
     1201 acacgacctt cacaatggtc ttgttgcgct gttcgatact ccgaatcacc cactgcgccg
     1261 cccgcaattt gtcgtccaga taggccttgg tctccgccga ccccgattgc cctgacgaca
     1321 tcaattgctt gtagtacggg ctgatccgca tacgcggcag gccgtcgtcg ttgagcaaca
     1381 cttcccacac cccttcgttc ttgacgacaa agacatccgg gacgatcgcg taattctgtg
     1441 tgttggagaa ggggcgtcca ggcttcggct ccaacccttc gataatccgg gtcgcttcga
     1501 aaacgtcgtc catcgaaata ttcagcgcct tcgcgatgcg gttgtactgt ttcttctcca
     1561 gatccttcaa atggtggagc acgatggatt ccagcaccgt ccccttcagc actcccgggc
     1621 gcgaccctaa ggagccaatc tggctccgtc ccaaaaactt cagttgaagc agcagacatt
     1681 cggataagtc ccgtgccgcc acaccatttg gatcgaatcc ctgaacatct ttcagcaccg
     1741 actcagcttc cgccaccgaa tagtccgtgc cagcgaccag ttcatccaag ctcatccgga
     1801 gatacccgtc gtcatccaga tttccgatga tcaggcgtcc gatcgctttt tctcgatccg
     1861 agaggcccga taacgacaac tgccagacaa ggtgatcttc taacgacgtc gacttcgcca
     1921 cggtttgttc atacgaggga aactcctcct tcgaggaaga gctgtactcg gtttcaccgc
     1981 gacgcatgtc cgtatcgaag taatcttccc agctggaggc cgaaaattct tcggcgtttt
     2041 ccccgctttc ggcaggctta tcgtcgccct ctcctcgcac ttcactggat acggtggtcg
     2101 acgtatcggg ctgttctcgc tccgccgtcg ccgtctcctc actttcttcc gtctccgtcg
     2161 ccaattcatc caacaacgga ttttccatga ggtgctgttg cagactttgc tgaagctcca
     2221 aacgagacag ttgcagcaat ttgatcgcct gctgcaattg cggcgtcata atcagttttt
     2281 gactgagcct gagatccagt cgcagcttca tcgcactctc actccctgcc gctacaggcg
     2341 gaatccttcg cccaaataca cggccctggc catttcgctt ttctcgatta ccccgggcgg
     2401 gcccgcttcc aggatcgtgc cttcattgat gatgtaggct cgatcggtaa tcgacaacgt
     2461 ctcccggaca ttgtggtccg taatcaacac gccgatattc cgttgcttca accggataat
     2521 gatttgctgg atatcggcca ccgcaatggg atcgataccg gcaaaaggct catccagcag
     2581 catgaatgag gggctggtcg ctaatgctcg cgtgatctcc agacgtcgac gttctccacc
     2641 cgacaaggca taggccttac ttttgcgaat atgcaccaga tccagttcct tgagcaatgt
     2701 ctcgacccgc tccatgcgct cacttctcga atagcccagc atctccagaa tcgcaaggat
     2761 attgtgttcc actgacagcc ggcgaaacac cgacgactcc tgcggcaaat acccgatgcc
     2821 cctccgggcc cgtttataca tgggcaaatc ggttatttcc tggccgctta aggcaatcgt
     2881 gccttcatcc ggctgacaca gtcccaccat catatcgaag atggtcgttt ttcctgctcc
     2941 attgggcccg agaagaccga cgacttcgcc ggcctgcaca tcgagcgaca caccctttac
     3001 cacttttcgt ccgcgaaaac tcttcaccag gccgttggca gccaggcgat ccggcatgcc
     3061 cgttgatgtc gcaccctggg cgaccgatgt ctgggcgtcg gaagacatcg ggatcaccgc
     3121 ttgccaccgc tttcgccctc gatgaccacc tgagagtccc cctccaccac actgcgatcc
     3181 tcgtccagga acataatgat ccgcttgccg gatacgcgcg tccccttctg ccaagccacc
     3241 ggatcacctg tcagaatgat tttcttttca tcttcgtagt acacggcctt ctggcaggtc
     3301 gcgcgcccat cttctttctc aattctcact cggcccgtcg cttcgaccat ccggatactg
     3361 cgctccgaga ccgcgggtgc cgcatcctgc ccttgtggtt ccggcttgtt cgcactcgaa
     3421 ttgttcttcg gcttggttgc accgttagca ttcttgctgt cggccccatt gcggttggga
     3481 tgaaacgaga ccaccatatg atccgaatgc accaccagca ggccacgagt caagacaacc
     3541 gccccgtcaa aaatcgcggt atttgcctga ttactgaccg tcatggtgcg agacgtaatc
     3601 gtcgtcggtg caggattgga agtcgactct ttcgcaacgc ttggctctgc cccatgcgcc
     3661 cagggaaggg tctgcagcgc gatgccgcta agaagcaggc accacatcga cgcgcacgtc
     3721 gtccaacacc tgaaactctt ctgtgtccat ttttcccagc aatcccctgc cggtgacttg
     3781 caacccatgt ccgttaatcg tgacatggtc tggagtttga atttcatgcc gcgcatcagt
     3841 ccacacaaga tgatttgtct gaatggtata gccactttga gtttcaacca caatcggcgt
     3901 ggtcctgttg gaaagttgaa aattcttggt ctgtgtatca agcgtgccct catccccgga
     3961 caaggtcagt tcctttccct gagccccgta catggtaatt tggacattgt tcagtattgc
     4021 ccggctgtcc ttttcaaaca gacgcgcctg ttcggcttga actttccact gaacaagatc
     4081 gccttttgtc tgggtgaagg taaagtcttg aattctggcg tcggccgatt cggtgtcaac
     4141 cggcgcgacc gaacgcgacg aagatccagg attggagcgt gtgacgagca aatacccaag
     4201 gaatgcagca agcaccacac tcagagctag aaggcctcgt cgaatccaac gttcccacat
     4261 ggcaaaaagc ccctaattta aagagcttcc caggtcacag tcggtggcat gatagtagca
     4321 ttccaccctg gccaagtaaa cctacagccc gcgcatggcc tgagaaaatg atcggagata
     4381 acgatgcaga gtgaggtggg gagggaacaa ggccacgacg cccctgaggt cgtcgtggct
     4441 ctcgatgtct agctttctgc ttgggctgaa gtcttttctg tcgtatcttt cttctcagcg
     4501 accgcgacgt ccgccgatgg cacggccttc ggcttctccg gcctggtcac caattcaatg
     4561 gccaccatct ccgcggcatc accgatccgc cgacgggtac ggatgatacg cgtgtagcct
     4621 ccggggcgct cacgaaagcg cgatgcaaca tcgctgaaca gcttcgccac caccgccttg
     4681 cttcgcaaga agctcagagc gcggcgacgg gcaggaagcc ccccctcttt cccaagagta
     4741 atcatgcgat cggtaaagcc acgaacttcc ttggccttgg cttccgtcgt ttcgattcgc
     4801 tcatgttcga gtaacgaagt caccagactc cggaacagag cccatcggtg tttagtttga
     4861 cgtccgagct gtcgaccctt ctttttgtgg cgcacggtag tcctgttcct ttcttactct
     4921 g
//
LOCUS       DAORQN010000107         4857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_679_length_4857_cov_13.997293, whole genome shotgun sequence.
ACCESSION   DAORQN010000107 DAORQN010000000
VERSION     DAORQN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_4857_cov_13.997293"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..301)
                     /locus_tag="PK614_06680"
     CDS             complement(5..301)
                     /locus_tag="PK614_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRB81933.1"
                     /translation="MFVMSNVLQGTATVLDTVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLERATEPVLTPIRRLVGWRMGMDLSPMIAILILVFLQYAVVQSLRDIAVRMH"
     gene            complement(301..1119)
                     /gene="proC"
                     /locus_tag="PK614_06685"
     CDS             complement(301..1119)
                     /gene="proC"
                     /locus_tag="PK614_06685"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRB81934.1"
                     /translation="MSSSMLTKPIAFLGGGQMAEALIGGLLAAGACEPASICATDPVA
                     ARRDALKSRFGIRVGDENAKAVREADLVVLAVKPQAMPAVLSDAGQACAGKFIVSIAA
                     GVTTAWIRERIVTPRGIVRAMPNTPALVREGVTALTCQPDLQSDDLAAVRTLFEAVGS
                     VVSVEERLMDAVTGLSGSGPAYVFVAIEALADGGVRMGLPRATAELLAAQTVLGAARM
                     VLERGEHPAKLKDQVASPGGTTIAGLYQLEAGGLRSCLMAAVEAATKRSQELGR"
     gene            complement(1112..1819)
                     /locus_tag="PK614_06690"
     CDS             complement(1112..1819)
                     /locus_tag="PK614_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRB81935.1"
                     /translation="MDAALGTIADRVRTVLDEMQRAAARAGRPPESVRLIAASKTVSV
                     ERLRQAVDAGIRHLGENRLQEALPKIDTLGREGVVWHFIGTLQRRKVKSVVGRFETIH
                     SVDSLALAEEIDRQAKTAGLRQRILLEVNLAGEASKGGFEPTTLVAALESLSGFEHLD
                     ICGLMAIPPPTPTAEDARPYFRQLRTLAQTLTARGYKNINMQELSMGMSHDYPVAIEE
                     GATYVRVGTAIFGARDE"
     gene            complement(1920..2663)
                     /gene="pgeF"
                     /locus_tag="PK614_06695"
     CDS             complement(1920..2663)
                     /gene="pgeF"
                     /locus_tag="PK614_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRB81936.1"
                     /translation="MVSEVITAPSFATAAEGVEHFFGTRLSSVSVTPGRAAVPGALHR
                     ERGASVILSVKQVHGTDALIVDRPVEEGDAFEGGWDALVTDQPGLMVTVRTADCVPVL
                     LHDPERRVVAAIHAGWRGAVAGIVPKTVALLVNRFGATVDRLQMAIGPSAGPCCYEVD
                     EPVLARLREVFPEWQSVVKPVSSQKAHLDLRAFVRRQALADGLAAERVATVDACTICQ
                     PDLFFSYRREGVVKATMVSGIALLPRRPG"
     gene            complement(2704..3906)
                     /gene="ftsZ"
                     /locus_tag="PK614_06700"
     CDS             complement(2704..3906)
                     /gene="ftsZ"
                     /locus_tag="PK614_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRB81937.1"
                     /translation="MFSFQEEPQSPVRIKVIGVGGAGCNAVNTMITGGLCRVDFVAAN
                     TDVQALERSQASYKIQIGPERTRGLGAGAKPEVGRDAALESKDEIRESLVGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVAVVTKPFQYEGHRRMSHAEEGIRDLGRHVDT
                     LLIIPNQRLLGIVDKATPLLDAFKVADDVLRQAIQGIADVITTIGLVNVDFADVRTIM
                     AHTGRAVMGMGIGRGANRAQEAAQKAICSPLLEEGSVEGARGVLLNITGGPNMSLHEV
                     EEAASIVQHAADAEANIIVGQVINPEIGDDLIVTVIATGFEREEQPAARPAVTAERPA
                     ARTPNGRPAQQVLTGVHAAGSDRPHKDLDRPTFLRRMGETREAVERIAVVGDDEWDVP
                     TFLRKQAD"
     gene            complement(3961..>4857)
                     /gene="ftsA"
                     /locus_tag="PK614_06705"
     CDS             complement(3961..>4857)
                     /gene="ftsA"
                     /locus_tag="PK614_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRB81938.1"
                     /translation="ARTLAVIPHERRILHVLPREFMVDDQEGVREPLGMSGNRLEVNV
                     HVITGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSAEERELGVAMVDLGGGTT
                     DLAIFLDGSIRHTAVLPIGGQNLTKDLAIGLLTSQTDAEKIKVQHGIARTELVHGHQM
                     VEVPSVGDRPPRQFTRRDIAEILEPRVEEMFDLVKREIVRAGYEGMLGAGVVITGGTS
                     LLEGMPDAAERGLNLPARRGMPTGIGGLRDIVSNPMHATGVGLLLHARQHADNLEAAG
                     IRHGKGLGKVFDRMRSWMFEFF"
BASE COUNT          934 a         1714 c         1328 g          881 t
ORIGIN      
        1 acggttaatg catccgcacg gcgatatccc gtaacgattg aaccacggca tattgcaaaa
       61 agacaagaat cagaatcgcg atcatcggcg acagatccat gcccatgcgc catcctacca
      121 gccgacggat cggcgtgagc acgggctccg tcgcgcgctc caaaaactgc acgatcgggt
      181 tccacggatc cggattgacc cacgaaatca acgcgcgggc gatgatgacc cacatgtaca
      241 gccacaacac cgtatccagc accgtggcag tcccctgcag cacattgctc atcacaaaca
      301 tcagcgtccc aactcctgtg aacgttttgt ggccgcctcg acggccgcca tcagacagct
      361 ccgcagccca ccggcctcca attgatataa cccggcgatc gtggtgccgc ccggcgacgc
      421 cacttggtct ttcaacttgg ccggatgttc gccccgctcc aaaaccatcc gcgcagctcc
      481 caggaccgtc tgcgcggcca ggagttccgc ggtcgctcgc ggcaagccca tccggacgcc
      541 gccgtcggcc aatgcttcaa tcgcgacgaa tacataggcg ggtccgcttc cgctcagacc
      601 ggtcaccgca tccatgagtc gttcctcgac cgacacgacc gacccgaccg cctcaaataa
      661 tgtccgcacc gcagccagat cgtcagattg gagatcaggc tgacaggtca acgccgtcac
      721 tccttctcgc acgagggccg gcgtgttggg catcgcccgg acgattcctc tgggcgtcac
      781 gatccgctcg cgaatccagg cggtcgtcac accggcggcg atggagacga tgaattttcc
      841 ggcgcaggcc tgccccgcat cactgagcac cgccggcatc gcctgcggtt tcaccgcgag
      901 aaccaccaga tcggcctccc ggacagcctt cgcattctca tcgccgacgc gaatgccgaa
      961 gcgcgatttc aacgcatcgc gacgcgcggc gaccggatcc gtcgcgcaaa tggacgcagg
     1021 ctcacaggcc cccgcggcca gcagcccgcc gatcagagcc tccgccattt gcccgcctcc
     1081 gaggaatgca atcggttttg tcagcattga actactcatc tcgcgctccg aaaatcgccg
     1141 ttccgactcg cacgtacgtc gccccttcct caatcgcaac gggatagtcg tgcgacatac
     1201 ccatcgagag ttcctgcata ttaatgttct tgtacccccg cgctgtcaat gtctgagcga
     1261 gggtccggag ttgtcgaaag tagggacgcg cgtcttccgc cgtcggagtg ggtgggggaa
     1321 tggccatgag gccgcaaata tccagatgct cgaacccgct cagggattcc aaggccgcca
     1381 ccagtgtcgt cggctcaaat cctcccttgc tggcctctcc ggcaagattc acttccagca
     1441 ggatacgttg ccgcagcccc gcggtcttcg cctgccggtc gatctcttcc gccagagcga
     1501 gactatcgac ggaatgaatc gtctcaaatc gcccgacgac cgatttcacc ttgcgccgtt
     1561 ggagggtccc gataaagtgc cagacgaccc cttcgcgccc taacgtgtcg attttcggca
     1621 aggcctcctg gagacggttt tctccaagat gtcggatgcc agcgtccact gcctgacgca
     1681 gccgttcaac tgacacggtc ttggacgcgg cgatgagacg aacagactcc ggcgggcgcc
     1741 ccgcacgggc ggccgcacgt tgcatttcgt cgagcaccgt tcgaacccgg tccgcaatcg
     1801 ttcccagcgc tgcatccatc ctgccgcctt gcggattgct ccacgtgtac catcacccga
     1861 tgccgccgtg aggacgcaat aagacaggct ccgggaatga agtccgtaac gagtcgatct
     1921 caacccggcc ggcgaggcag gagagcaatg ccactcacca tcgtcgcctt gaccacgcct
     1981 tcgcggcggt agctaaaaaa caaatcgggc tgacagatgg tacaggcatc gaccgttgcg
     2041 acccgctctg cggccagccc gtcagccagc gcttgccggc gcacaaaggc gcgcagatcc
     2101 agatgcgcct tctggctgct caccggtttt acgaccgatt gccattcggg aaagacctcc
     2161 cgcaatcgcg ccaggaccgg ttcatccacc tcgtaacagc agggaccagc ggaagggccg
     2221 atcgccatct gcagccgatc gacggtcgcg ccgaaccggt tgaccagcaa ggccaccgtc
     2281 ttcggcacaa taccggccac cgcaccccgc cagcctgcat ggatcgccgc aaccacccgt
     2341 cgctccggat cgtgcaggag cacaggcaca caatccgcgg tccgcacggt caccatcagc
     2401 cccggctgat cggtcaccaa cgcgtcccag cctccttcga aagcgtcgcc ctcctcgacc
     2461 ggccgatcga cgatcagcgc atccgttccg tgaacctgtt tgacggacag aatgacgctc
     2521 gcaccccgct cgcgatgcag ggcccctggc acagcggcac gacccggtgt caccgagacg
     2581 gacgacaacc gggtgccgaa aaaatgttcc accccctccg cggccgtcgc aaacgacggc
     2641 gcggtgatga cctctgacac catccccgtt ccaaccctcg cagtgaccga taccgcttcg
     2701 tgctcagtcg gcctgctttc gcaggaaagt cggcacatcc cactcatcgt cgccgaccac
     2761 cgcgatccgc tccacggctt cccgggtctc gcccatgcgg cgaaggaagg tcggccgatc
     2821 caagtcttta tgaggtcgat ccgatcccgc ggcatgcacg ccggtcaaca cctgttgcgc
     2881 cgggcgtcca ttgggggtcc tcgctgcggg acgttcggcc gtgacggccg gtcgcgcagc
     2941 cggttgttct tcccgctcga atccggtcgc aatcacggtc acgatcagat catcaccgat
     3001 ctcgggattg atgacctgtc cgacgatgat gttcgcttcg gcatccgccg catgctgaac
     3061 gatcgacgcc gcttcttcca cttcatgcag cgacatattc ggaccgcctg tgatattcag
     3121 caggacgcca cgagcgcctt cgacgctgcc ctcttccaac agcgggctgc aaatcgcctt
     3181 ctgcgccgct tcctgcgccc ggttggcgcc acgcccgatc cccatcccca tcaccgcgcg
     3241 ccccgtgtgg gccatgatgg tccgcacatc ggcgaagtcc acgttgacca gcccaatggt
     3301 cgtgatcacg tccgcaatac cttgaatggc ctggcgcaat acgtcgtccg ccaccttgaa
     3361 cgcatccagc aacggggtcg ccttgtccac gatcccgagc aaccgttgat tcgggataat
     3421 caggagcgta tcgacgtgcc ttccgagatc gcgaatgcct tcctcggcat ggctcatccg
     3481 tcgatgtcct tcatactgga agggcttggt cacgaccgcg acggtcagga tgcccagttc
     3541 acgcgcaatg ctggccacga tcggagccgc gcccgtcccg gtgccgccgc ccatgccggc
     3601 ggtgacaaac accatgtcgg cgccgaccag actctcgcga atctcatctt tactttccaa
     3661 cgcggcgtcg cgtcccactt cgggcttggc gccggcgccg aggccgcgag tccgttccgg
     3721 accgatctga atcttatacg acgcctggga ccgctcaagt gcctgcacat ccgtattcgc
     3781 ggccacgaaa tcgacccggc acaatccgcc ggtgatcatc gtattgacgg cgttgcaccc
     3841 cgctcctccg acaccgatca ctttgatgcg aacgggtgat tgcggctcct cttgaaatga
     3901 aaacatcgtg gcacctccct tgacgatggt gcggacgtgg tcttaagccg tccgcaaaag
     3961 ttagaaaaac tcgaacatcc acgatcgcat gcgatcgaac actttcccca accctttgcc
     4021 gtgacggatg ccggcagcct ccaagttgtc ggcatgttga cgtgcatgca acaacagccc
     4081 gacaccggtc gcgtgcatcg ggttgctgac gatatcgcgc agcccaccga tgcccgtcgg
     4141 catgccgcga cgagccggca gattcagccc tcgctccgca gcatccggca tcccttccaa
     4201 taatgaggtt ccgcccgtga tcaccacgcc ggcaccaagc atgccttcat agccggctcg
     4261 cacgatttcg cgcttcacca gatcgaacat ttcttcgacg cgcggttcga gaatctccgc
     4321 gatatcacgg cgcgtgaact gccgcggcgg ccggtcgccg acggacggca cttccaccat
     4381 ttgatgacca tgcaccagct cggtccgggc aatcccatgt tgcactttga ttttctcggc
     4441 gtcggtttgc gaggtgagca gaccgatagc cagatcctta gtcaaattct ggccgccgat
     4501 cgggagcacc gccgtgtgcc ggatgctgcc gtcgaggaag atcgccaagt cggtcgtgcc
     4561 gccgcccaga tctaccatgg cgactcccaa ctcccgctct tcggcgctca acaccgcctc
     4621 gctcgaggcg aggggttgca ggatgatgtc cacgacgtcg agcccggccc ggttcacgct
     4681 cttgatgatg ttctgcgccg acgtcaccgc accggtgatg acatggacgt tgacctcaag
     4741 ccggttgccg gacatgccca gcggctcgcg cacgccctcc tgatcgtcca ccatgaattc
     4801 gcgcggcaac acgtgcaaga tgcgacgctc gtgaggaatc acggcaagcg ttcgcgc
//
LOCUS       DAORQN010000108         4838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_685_length_4838_cov_15.711896, whole genome shotgun sequence.
ACCESSION   DAORQN010000108 DAORQN010000000
VERSION     DAORQN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_4838_cov_15.711896"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            703..900
                     /locus_tag="PK614_06710"
     CDS             703..900
                     /locus_tag="PK614_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81939.1"
                     /translation="MGDGRSVGLVCVVGGLVGVDGLVADGGLTVGLVGAVGGLVADGG
                     LVVVPSWAKIDPFTSLQPISL"
     gene            complement(1395..2972)
                     /locus_tag="PK614_06715"
     CDS             complement(1395..2972)
                     /locus_tag="PK614_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81940.1"
                     /translation="MGSKVVAASHSPVVKLTNAVHFLGGDGEDIAAESGQYVVESVVG
                     RKIRLSTGGRSSLIVDAITTTHTQDLATPLALTVIDDDEDLLHLVLLLPNGTALDAIG
                     SQKGTRPLGSSLVPVSPVQIQGALLQKTAVVRDHRDAFYFDLAYFHAPIHYQFTHSAR
                     AIGDYITRFDFDGNWAGRDNWDNMQHLPLPAYVYYSVVETVTHWFIIYMSFHPQDWAK
                     PSDLDQEHENDMEGELSIIRRDGSRFGKFEGMVTTFHHDFYSYTPVGSALRDGPLVVT
                     QEVDANGNRVRVRQDIDGEVRMQSYEGSLRPLTNQEDRGHGLKAYPYALSVRPGIAAP
                     AGSIPSLLPGRDLIVYYPSRTHSEVPSSGNDRNVKYSLMDIFAPNGLWEHQLADIRET
                     NDPRPSGHTFISWGTVVGNESGSCGAGYLRWCVANADLAPWLWDDTGEVVGGIRFSEA
                     DAGVYAGEMALNPAHLTKFYFTGLEPFSETYTRNRYLSDLCQRGYHQGNLPKGWPSRL
                     NLDQLYSKLNGGCQEHP"
     gene            complement(3142..4578)
                     /locus_tag="PK614_06720"
     CDS             complement(3142..4578)
                     /locus_tag="PK614_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81941.1"
                     /translation="MAVSLLVVVQASALAQTENTSLRGITKRDNVPIDIKAGETKQVQ
                     IPLSTTSSGSLIRVRLISSTPSPNVKVKMSAGGKTLKLLRLFKSSNSWVFKANASATN
                     AVIDIQSASTEPVQGKLLIAVVDSDERRKANASRAAAPKTQSVRKEVSPDVLMAVSHT
                     LKRAASNFPGTKSTQEQAIAQAINQGKISRDILKTMSKALEKRKPQFAPRVQIAELSN
                     LPPNRPVPKVALDKVLTVKKPLSLPHDDVGPAENSPIDPNAKYTIRIAAIQSQRCADD
                     VGWETGCDDEEPFIAWFMVGADYLRTGVTDSAEIQKGQTHLFKRDTMVLSGAPDNSQA
                     IRPSWPIAFDFQIIEDDPEGPTREQLNSALKTGAEIAALWYTGGLSGAVKDPEMMGDA
                     VDLARYLIDSVQCLSGCGDDEFGAYKTVLSEQTAANLTNGKPLEIVSQGTWDWGGNYD
                     KYHKGIVEDKTSNGKATNWKVMWEMTRR"
BASE COUNT         1121 a         1247 c         1222 g         1248 t
ORIGIN      
        1 agtcgcatcc ggactccaaa cgtagctgct actcaagacg gggggacgtc gtatctcccc
       61 ccaaaacttt cgttgttttg gaccacggaa gacgacaatt cagcttgagg ccgggcttac
      121 agtgtttgtt ggagggaacg gatcgggaaa gaaagctgtg ttccatgggc tagctcggct
      181 tttcggacta acggaagcac agcgacggat tcgacgtaac gattttcatg tgccggtaga
      241 tcaagaacag atccgatctg gggcgacttt gagattgaga tgatcctgtg ttttccggaa
      301 ctagagggtg tggcagagga tcactggggt gatgcagtgg ccgagtcttt ccttcacatg
      361 gctgcatctg gtgaaggggc ccattaaagg caagacttca actgagaaca acctggaccg
      421 ataacgggac gcccgatgga aatatcgaag aaaagataaa atggattcat gccttgagca
      481 tgttgcgtac gaaggacatt ccggtggcac ttcatgaaac ggtgctgcgg gcttctattg
      541 ggtggcaaca ctcaggggac acatagccat ttcggcagcc gcccctatca aacggaatcc
      601 tctttgacca gataagcgat tgcagtgtca tatgacactc ctctgcctta cttgtctttg
      661 tccacctggc ttgattgctg ctcgggttcg accccaaggg gcatggggga cggaaggagt
      721 gtcggactgg tctgcgtggt cggcggcttg gtaggagtcg atggcttggt tgcggatggc
      781 ggcttaacgg tcggattggt cggcgcggtc ggcggcctcg ttgcggacgg cggcctcgtt
      841 gttgtgcctt cgtgggccaa gatagatccg tttacttctt tacaaccaat ttcgttgtaa
      901 tgtagacgaa gactccgcat ttggccgcgt tcttcatccg tcaatggccg acggtccgac
      961 aacgcaacgt aaccttgata ttgcttcttt atcgccctgc actcagcgat ggccgaggtt
     1021 atgtcgtcag caatgatttc tctcgctcca gttaaaggag cgtaatgtgg cgaacacgat
     1081 atcactacag ttacaacagt cgccatttgc atgcgtgtat ttaccatcac tgctccttat
     1141 cctgaaattt aacgcctggc tggcacggca tccgagccaa tctcaaataa gacgaacaca
     1201 cggcaaggcc atttaattta gttagtccca agttgtaacc gccaacctct ttgcttcctt
     1261 cgtcatcaca attcctcggg ctcttggaat acaacctacc tttacccaag gagctgtcga
     1321 tgtatgactt atccgccctt ttcaagaggc ctagtgcccg taagatggct acttcaaaca
     1381 gagtcgaccg ccgttcaggg atgttcctga catcctccgt taagcttcga atacaattga
     1441 tctaaattca gcctgctagg ccagcctttc ggcagattcc cttggtggta accacgctgg
     1501 catagatccg aaagatatcg gttgcgagtg tatgtttcgc tgaatggctc caatccggtg
     1561 aagtaaaact tcgtcaggtg agccggattt aacgccattt ctccagcata aactccagca
     1621 tcggcttccg aaaaacgaat gcctcccacg acttctcccg tatcgtccca gagccaaggt
     1681 gcaagatcgg cgttagccac acaccatctg agataccctg caccgcagct accactctcg
     1741 tttcctacta cagtgcccca agagataaat gtgtgacctg acggacgcgg atcatttgtt
     1801 tcccgaatat cagcgagttg gtgctcccat aacccgtttg gggcaaaaat atccataaga
     1861 ctgtatttca cgttacgatc gttcccggag gagggtactt ctgaatgcgt gcgagatggg
     1921 tagtacacaa tcaggtctcg gccaggcagc aaggaaggga tgcttccggc tggcgcggca
     1981 ataccagggc ggacgctcaa ggcgtaagga taagctttta gcccatgtcc ccggtcctcc
     2041 tggttagtca gcggacggag cgacccttca tagctctgca tccttacttc cccatcaata
     2101 tcctgccgga cgcggacccg attcccgttc gcatcgacct cctgggtgac caccagcggt
     2161 ccgtcccgca aagcacttcc gactggtgtg taggaataaa agtcgtgatg aaacgtggtg
     2221 accatccctt cgaatttgcc gaatcgggaa ccatccctcc ggataatact gagctcgccc
     2281 tccatatcgt tttcatgttc ttggtcgagg tcgctaggct tggcccagtc ttgaggatga
     2341 aaggacatgt atatgatgaa ccagtgcgta actgtctcca cgacggagta ataaacgtac
     2401 gccgggagtg gaagatgttg catattgtcc cagttgtctc gacctgccca attaccatcg
     2461 aagtcaaagc gagtgatata gtctccgatc gctctggctg agtgagtgaa ttggtaatgg
     2521 atcggagcat ggaaatacgc taagtcgaaa tagaaagcat ccctatgatc tctgactaca
     2581 gccgttttct gtaagagtgc tccttggatc tgaaccggtg agacggggac gagactactt
     2641 cccagggggc gcgtgccttt ctgggaccca atcgcatcaa gggctgtgcc attgggtaac
     2701 aacagcacta ggtgtaagag atcctcatca tcgtctatca ctgtgagtgc gagcggtgta
     2761 gctaaatctt gtgtatgcgt ggtggttatg gcatcgacaa tcagagatga tcgccctccc
     2821 gtggacagtc gaatctttct tcccaccacg gattcaacga cgtactgacc ggactcggcg
     2881 gcaatgtctt cgccatcacc acccaggaaa tggacggcgt ttgtcagttt gacgacagga
     2941 gagtggctcg cggccacgac tttgctaccc ataagcgtca ccaggagtgt gaccgcgatt
     3001 gaccaagtaa ttgcctttgt ggatcgtctg aacaattgat ttctcattct ttcacgttgt
     3061 ctagggattc atgggcagtg tatgaaccta ccatacatcc ctagaccatg gttcaggacc
     3121 aaatatcata aaggataatt tctacctcct ggtcatctcc cacatgactt tccagttagt
     3181 agctttgccg ttagacgttt tatcttctac tatgcccttg tggtatttgt cgtagttgcc
     3241 accccaatcc caggtgcctt ggctaactat ctctagaggc ttgccattgg tcagattagc
     3301 ggccgtctgc tcactaagaa ctgtcttata ggcgccgaat tcatcatcgc cgcatccaga
     3361 caagcactgt acggagtcaa tcaaataccg agcaaggtcg accgcatcgc ccatcatttc
     3421 cgggtctttc acggcgccac tcagcccacc cgtataccat aatgcagcga tttcagcccc
     3481 cgtcttgagt gcggaattga gctgttctcg cgttggtcct tcaggatcgt cctctatgat
     3541 ctgaaaatca aacgcgatgg gccaagaagg ccggatggct tgagaattgt ccggagcccc
     3601 actaagaacc atcgtatctc tcttgaataa atgcgtttga cctttttgga tctcagcgct
     3661 gtctgtgact ccagtgcgta aatagtcagc gcctaccata aaccaggcta tgaatggttc
     3721 ttcatcgtca catccagttt cccatcccac atcgtctgcg catcgctgag actgaatggc
     3781 tgcgatgcga atcgtgtatt tcgcattagg gtcgattgga ctgttttcag ccggtccaac
     3841 atcatcatgg gggagactga gtggtttctt gactgtaagt actttgtcca gtgccacttt
     3901 gggcaccggt cggttcggtg gtaggttcga tagctctgca atctgcactc ggggagcaaa
     3961 ctgaggtttc cgtttctcca aggctttgga catggtttta agaatgtccc ggctgatctt
     4021 tccttggttt atcgcctgag ctattgcttg ttcctgagtt gactttgttc cgggaaagtt
     4081 gctcgccgcc cttttcaggg tatgggacac ggccatcaac acatccggac taacttcttt
     4141 acgaacgctt tgtgttttag gtgccgcggc tctggatgcg ttcgctttac gccgttcatc
     4201 ggagtccaca actgcgatga gtaacttgcc ttgcaccggc tcagttgatg cactctgaat
     4261 atcaatgacc gcgttcgtgg ccgatgcgtt tgccttaaac acccaggaat ttgaactttt
     4321 gaacagacga agcagcttca aagtcttccc accagcggac attttaactt ttacattggg
     4381 tgaaggtgtt gatgagatca acctgacccg aatcaaggat ccactggaag ttgtcgagag
     4441 gggaatctgg acctgtttcg tctcaccagc cttaatatcg atgggcacgt tatcacgttt
     4501 cgtaatgcca cgaagtgatg tgttctccgt ttgagcaagt gcagatgcct ggacgaccac
     4561 caacagactg accgccataa ggatgatcgc aagaacgaat ctatgactcg acatcctgaa
     4621 cctcccttct gacaaaaatg ctgcctggaa gatcctctac aaagctagaa agccgaggca
     4681 aaaaccatac taattcgtta caacgatatg gttgcattgc aagtgcttgc gctagctagt
     4741 gatacgtatt tgttccatcg cctttacctc atggcaacgt atccgaaaag atacggacgg
     4801 aatccatttg gatacggttt gaaggtactg aagtctta
//
LOCUS       DAORQN010000109         4825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_689_length_4825_cov_7.789937, whole genome shotgun sequence.
ACCESSION   DAORQN010000109 DAORQN010000000
VERSION     DAORQN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_689_length_4825_cov_7.789937"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            466..750
                     /locus_tag="PK614_06725"
     CDS             466..750
                     /locus_tag="PK614_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81942.1"
                     /translation="MSVEHPAKPPAPVHYVSSEEWIARISRLQDTLTVCPTDVLARCE
                     LATLLEKLEQPEEALVHWQVVLSCDSNSLNAREGVARCRLQAGRPLQSHM"
     gene            759..1040
                     /locus_tag="PK614_06730"
     CDS             759..1040
                     /locus_tag="PK614_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81943.1"
                     /translation="MPTLWGCFDQVDKGLSGTCWILLRTLLPGGTTIRIRALVGEQAL
                     IARGTERVELSRVKVGEFVEVTYHRGPAGFMEADTIYVRSDQDFAPEES"
     gene            1634..2680
                     /locus_tag="PK614_06735"
     CDS             1634..2680
                     /locus_tag="PK614_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB81944.1"
                     /translation="MIWLPFASWLLPHSTQDFDLAVKVAGSGAPQLRQDWLPLAHLLV
                     EEGLAVMAGMDSSAWAYEEGPVTDGGRVVGTITLDGQVPKPKGYNLTTLPDQFYCGRI
                     SDGQGWRILQPFQVGPGGEFRDVVVYLEGIDKGKPFSEGSVPQVDARDCLFEPFTTVV
                     RDDQSVTVVNMDPVMHDIQAYETSQLGARVLFNLPLPMNPQHPRNLKDRSDAGMYHKH
                     MAGPSMKQLVNLSKGRRIFVMQCGFHAYMESWGLAITNPYFAKTDEHGRFTLTDVPPG
                     TYKLIVWHPYIRTTVEQTVTIGPKGTVDAAIGVPAPTGRLYANEVMEHPYNRYNVLEE
                     TKREIDPMIHKQEH"
     gene            3302..>4825
                     /locus_tag="PK614_06740"
     CDS             3302..>4825
                     /locus_tag="PK614_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRB81945.1"
                     /translation="MTVLLAGLLTIYIAGVILPPCFPGKARIQNLVAHGLAAAAGFVG
                     MVLGLAGLLAPEPWTGSVESSIPFLSFAIRLDPLASFFVLTISVVGLAASIFALGYLA
                     ELYGRASIAAMGSLLNGFLLAMTLVVMADNGFFFLIVWELMSLLSYFLVVTEHEKPGV
                     REAGLFYLIMTHVGTAFIILTLLIFSQETGSFSFEAFRHPDQQLPEGLRSIAFFTALI
                     GFGAKAGIVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLIRVYFDFMGGVFPWWW
                     GFTLLVAGTVSALLGVMYALMEHDLKGLLAFHSVENIGIILLGIGAGMIFHTYGLHEL
                     AALGLLAGLYHTINHAAFKALLFLGAGALQFSTHTRHIEEYGGLLRRMPWTGAFFLIG
                     AVSIAALPPTNGFVSEWLVFQSLFLSFQLPTLFLKLMLPIAAAMLALTGALALACFAK
                     AFGMSFLAQPRSAHARHATEVPWSMRVGMGFLAGLCIVLGLAPMLVVPLLDRVTAPLT
                     "
BASE COUNT          909 a         1336 c         1460 g         1120 t
ORIGIN      
        1 ggccaatacc ctaagtcctc gatttgttgc gcatcgcacg catcgatctc ccgacttact
       61 ttttggcatt tcccctgcat agcctcgatc tgtcacacaa ccggtctctc catgatgtgc
      121 gcaggatgac tcacatggag aaccgccgta tcagcttggc tcgcccgtct ttctaatgac
      181 gttggagcga gtaatcgatg tgggttctgc cggttttcat cctccatcgg cgatggaact
      241 gggcgttaca catcctgatt ctggatacga gctgctctat ggccgccatg cccagcaaga
      301 tgaggtgatc gcagagtttg caaggcaact gcttaccagg aagaatccga caatcttccc
      361 ggggccgttg gggtcgggcg agggaatcca tggccagcct gagggtaagt gtccgacttg
      421 ggtggagagg cagccaggca gcagtgaagg aatccgagca taccgatgag cgttgaacat
      481 ccagcgaaac cacccgcgcc cgtgcactat gtgtcgagcg aggaatggat cgcgcgaatc
      541 agtcgtctgc aagatacgct gacggtgtgc ccaacggatg tcctggcacg ctgcgaattg
      601 gcgacgctat tggagaagtt ggagcaaccg gaggaggcct tggttcattg gcaagtggtg
      661 ctctcctgcg attccaacag tctcaacgcg cgtgaaggag tggctcgctg tcgtctgcag
      721 gcaggccgtc cgctacagtc ccatatgtga gagtgcgtat gcctacgctg tggggttgtt
      781 ttgatcaagt cgacaaaggc ctctccggca cctgttggat ccttctgagg accctgttgc
      841 cggggggcac aacgattcgg atccgggctc ttgtcggaga gcaggccctc attgcgagag
      901 gcacggaacg ggtagagctt tcacgcgtga aggtggggga atttgtagaa gtcacctatc
      961 accgggggcc ggccggcttc atggaggccg acaccattta tgtccgttct gaccaggact
     1021 ttgcgcccga agaaagctaa gtctgggttc tcgggaggct catggaacgg gctgtgaatc
     1081 gactgggaga gagacgactt gtgtcgtctc aacgagcatg gtgcccaatt ggagtggcgg
     1141 cattgtcgtg tggatgcttg tgaatggcaa ggcttgcggg caaggtactc aggattcgga
     1201 acaacgcccg atcatcctcg cctaaaccat gcgagcgcca ataggtcggg gaggccggca
     1261 cgatgagacg acaagaaaat caaagaggcc aacagcggtc tggggaacgc gacgcacgca
     1321 gtcaaacagg gagactcaag catatggtga aggacaggaa catcgtggtt gtgcagtacc
     1381 cacgaggtgt gaccgctctt gtctggctcg atccggccag ccgagaggtt gtgaccagcc
     1441 atgccgaatt gagttctctg cttcaccagg gagtgaggga ctggggaggc cgcctggtat
     1501 ttcccgaaag cggccaagac ttcttaaggg cggtttatga tcgcctcttc atcaatggtt
     1561 gcgccgtcca atggatgagt gcgtcggcta ccgtaaatgt tcaacgaagt taccgtgtct
     1621 agcgcggatc aagttgatat ggctcccatt cgcatcctgg cttcttcctc attccacaca
     1681 agacttcgat ctggccgtca aagttgcagg atcgggtgcg ccgcagctcc ggcaggattg
     1741 gctgcctctt gcgcatctcc tggtcgagga gggattggca gtgatggccg gaatggactc
     1801 ctccgcctgg gcctatgagg aaggcccggt taccgacggc ggccgtgtcg tgggcacgat
     1861 cacgctcgac ggccaagttc ccaagccgaa agggtacaac ctcacgacct tgccggacca
     1921 attctactgc gggcgcattt cggatggaca aggctggcgc atcctgcaac cgttccaggt
     1981 cgggcctggg ggcgagtttc gcgacgtcgt ggtgtatctg gaagggatcg acaagggcaa
     2041 acccttcagc gaagggagcg tgccgcaagt cgatgcgaga gactgcctct tcgaaccgtt
     2101 caccacggtt gtgcgggacg atcaatctgt gacggtggtc aacatggatc ccgtcatgca
     2161 cgacattcag gcctatgaaa cgtcccaatt gggtgcccgt gtgttgttca acctgccgct
     2221 gccgatgaat ccgcaacatc cgcgcaatct gaaggatcgc agcgatgccg ggatgtacca
     2281 caaacatatg gccggcccgt cgatgaagca attggtcaat ctcagcaagg gtcgccggat
     2341 ctttgtcatg caatgcggct tccatgccta catggagagc tgggggctgg ccatcaccaa
     2401 tccgtatttt gccaagaccg acgagcatgg ccggtttacc ctcaccgatg tgccgccggg
     2461 cacctataag ttgatcgtct ggcatcccta catcaggacg accgttgagc agacggtcac
     2521 cattggtcca aaggggactg ttgacgccgc catcggcgtt ccggcgccga cggggcggct
     2581 ctatgccaac gaggtgatgg agcatccgta caatcgctat aacgtgctcg aagaaacgaa
     2641 gcgagaaatc gatccgatga tccacaagca ggagcattga agcgggagtg agaggggcga
     2701 agcgcccaac actatctcgt ggagcaggat tgctgatagg cgatgagtgt gtggtgtagg
     2761 cgaggatctc gagagaggaa gcttgtcaaa atcagaaact gcgaatcgcc aaatcgatgg
     2821 gttagccatt ctgctgcatg gagtctcctc catgtcgtca ttacactggc ctgttctcca
     2881 gcgtctgcat gcggctgaat ctgacatcgg aaatgttgcc ggaactgact catctcacgg
     2941 tcggcatcct cgccttgtac catgcagtcg atagtctttc ccaggcgtat accggcaagg
     3001 cgatcgtgcc actgatgcct cctcttctct atcatgaaga gcatgagggt gccggtccga
     3061 ttggaaccgg cacggtccca cgggggaacc tgagttcgca tgaaatggtt gcatgggttc
     3121 tgcactatgc ccgttgtgca cggtcacttt tcggttatca gttgatgtgt caaaccgcat
     3181 gtcttaaggt tcctagaggc tgtcaagatt ctccggttca catctcgaag accaggggga
     3241 gtgatagaat gcattccgtg tctggttgtg ggggatcctc cttgaggttc atactgcaac
     3301 gatgaccgtt ctactcgccg gcctgctgac gatctacata gccggcgtca tccttccgcc
     3361 atgttttcct gggaaagcga ggattcagaa cctcgtcgca catgggcttg cggcggcggc
     3421 aggtttcgtc ggcatggtcc ttgggcttgc gggacttctc gcgccggagc cctggaccgg
     3481 ctccgttgaa tcctcgattc cctttctcag ctttgcaatc cgcctcgatc ccttggcctc
     3541 gttcttcgtg ctcacgatct ccgtggtcgg ccttgccgca tctatctttg cgctgggtta
     3601 cctggcagaa ctgtatgggc gggcatccat cgctgccatg ggctccctcc tcaacggttt
     3661 cttgcttgcg atgacgctcg tcgtcatggc tgacaacggg ttcttcttct tgatcgtctg
     3721 ggagctgatg tcgctcctgt cctattttct ggtggtcacg gagcacgaga agcccggcgt
     3781 tcgcgaggcc gggttgttct atctcatcat gacgcacgtc ggcaccgcgt tcatcatcct
     3841 cacgttgttg atcttttccc aggaaaccgg ttcattctcc ttcgaggcgt ttcgccaccc
     3901 cgaccagcaa cttcctgagg gattgcgatc gatcgcgttt ttcacggcct tgatcggatt
     3961 cggcgcgaag gccggcatcg tcccgttgca tgtgtggctg ccctacgcgc atcccgccgc
     4021 cccgtcccac atctcggccc tgatgtcggg ggtcatgatc aagaccgcca tctacgggtt
     4081 gatccgggtg tacttcgatt tcatgggcgg ggtattcccc tggtggtggg ggttcacgtt
     4141 gttggtcgcc ggtacggtct cggcgctcct gggtgtcatg tatgccttga tggaacatga
     4201 cctgaagggc ctgctggcgt ttcatagtgt ggaaaacatc ggcatcattc tgctgggaat
     4261 cggcgcaggg atgatcttcc acacctatgg cttgcatgaa ctggctgccc tcggattgct
     4321 ggcgggcctg taccacacta ttaaccacgc ggcgttcaag gcgctgttgt ttctgggcgc
     4381 cggagcgttg cagttctcca cccatacgag acacatcgag gagtacggcg gcttgctccg
     4441 gcgcatgccc tggacgggcg cctttttcct gatcggcgcc gtctccatcg ctgcgcttcc
     4501 gcccaccaac ggtttcgtca gcgagtggct ggtctttcag agtttgtttc tcagcttcca
     4561 actgccgacc ctgtttctca agttgatgtt gccgattgcc gccgcgatgt tggctttgac
     4621 gggcgcgctg gcgctggctt gttttgcgaa ggctttcggg atgtcgtttc tggcgcagcc
     4681 gcgcagcgcc catgcccgcc atgcgacgga agtcccctgg tcgatgcggg ttgggatggg
     4741 ctttttggca gggctctgca ttgtgctggg gctggcgccc atgctggtcg ttccgttgct
     4801 ggatcgggtc acggctccat tgacc
//
LOCUS       DAORQN010000110         4817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_691_length_4817_cov_5.748215, whole genome shotgun sequence.
ACCESSION   DAORQN010000110 DAORQN010000000
VERSION     DAORQN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_691_length_4817_cov_5.748215"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1083
                     /locus_tag="PK614_06745"
     CDS             169..1083
                     /locus_tag="PK614_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRB81946.1"
                     /translation="MRYLIATLTGAAIAMAASVGYAESVADKRGLTLEGARQVIAATV
                     DAARKADAPGGSIAVVDEGGNLLALERLDHTFAASANIAIGKARTAALFKKSTKVFED
                     AIKNGRTSMVTLGGDLQNFTPLQGGIPLEWEGKVVGAIGVSGAASAQQDEELAIVGAG
                     SLGSMEMTGRNNGHAPLSVTYIESRRVSAAFENGAVLVGEDENMMHAARNYMVHASHR
                     DKAGVVEIHEFDTDIVYVLKGSAQVITGGTPVGMKMIAPHEFRAPSVDDGEMRRLVPG
                     DVVIIPNGVPHWFKEVVAPFDYYVVKVR"
     gene            1416..2849
                     /locus_tag="PK614_06750"
     CDS             1416..2849
                     /locus_tag="PK614_06750"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRB81947.1"
                     /translation="MSIITDLAPSGPPNRTQDIDLHAGAADFDDSKWEVLKPIQLEER
                     RSAGRLCFSWYRTSITIPDSIGPFNPAGSTVVFEIAIDDYAEVWVDGRLPLVLGQPGG
                     QLIKGFNAPNRVVLTRNAKPGQRIQLAVFGINGPLSNPPGNFIWVRSATLDFYRPDHI
                     GPTQFVKTEILKTDPALDQIVAPGTQLEQLAGGFLFTEGPVWVPATADASGYLLFSDP
                     NANTIYRWSAEGQVSVFRAKSGYSGFNVGEYHQPGSNGLTLDRTGRLTINQHGNRRVI
                     RVEPRGNITVLADRYDGKRLNSPNDLVYRSDGALYFTDPPFGLPKVFDDPRKELPHSG
                     VYCVKDGVVKLVSTELDAPNGIALSPDEKTLYVNNWNDKRKVIVRYDVNSDCTLSNSR
                     LFFDMTNAPGSDALDGLKVDLQGNVYSTGPGGLWILSPDGRQLGLIKGPEDPHNMAWG
                     DDDGKTLYITALTGIYRIRLNIPGVRP"
     gene            2846..3853
                     /locus_tag="PK614_06755"
     CDS             2846..3853
                     /locus_tag="PK614_06755"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRB81948.1"
                     /translation="MSHLAGRGILLALLVLLVHVAAWAEPVPVTITSLDPRFDQLVPK
                     NTQVEKIADGFTWVEGPVWHKQGGYLLFSDIPANRVYQWKPGKGTSVYLKNSGYSGST
                     PFAGKEPGSNGLTFSADGQLYLCRHGDRQIGRLEADGRITPVAARFDGHLINSPNDLV
                     FKSNGDLYFTDPPFGLPGVFDDSGKAPVQGVYRLSHDGALTLLINDIKAPNGIAFSPD
                     EKTLYVSDVDPKRAAWLAYDVKADGTVADGRVFFDAMRWRKDPFFGPDGFKVDRKGNI
                     FGARPGGISVIASDGTLLGTIETRQPMSNVAWGEDGQTLFMTGGSSIYRLRVTTGADR
                     Y"
     gene            3919..>4817
                     /locus_tag="PK614_06760"
     CDS             3919..>4817
                     /locus_tag="PK614_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HRB81949.1"
                     /translation="MASHRRIPSAPPSPIPAEQQRLDEDAQRRRHWKRWGPYLSERAW
                     GTVREDYSPYGTAWDAFPHDQARSRAYRWNEDGLAGICDRHQIICFALALWNGRDPIL
                     KERVFGLTGNEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAAFPYTRLVEENRRRG
                     RHDPEFELIDSGVFDENRYFDVCVEYAKTTPEDLCIRIQVVNHGPEEAQLTLLPTIWF
                     RNTWSWGTDIRRPRFRQGESIDHMRVIETQHEHYGLRRLLCEGEPTLLFTENDTNSRR
                     LYGDQTGPRYVKDSFHDYVVQGE"
BASE COUNT          981 a         1392 c         1493 g          951 t
ORIGIN      
        1 gcgccatcgg ggtgagcgga gagacgccgg gccaggatga agacatcgcc ctagccggtg
       61 cggcggtcgc cgcgtcgttt gcgaagtagg agctgtcggc tttcggcgat ccgttcaaaa
      121 atacccatcg gcggctgacc gctcaatctc aggaagagga gaacgatcat gagatacctc
      181 atagcaacct tgacgggagc ggccattgcg atggcggcct ccgtcggcta cgccgaatcg
      241 gtcgccgaca agcgcgggct cacgcttgag ggagccaggc aagtcattgc agcaaccgtc
      301 gacgcggcgc gcaaggctga tgcgccgggc ggatccattg cggtggtcga tgagggcggg
      361 aatctgttgg cgcttgaacg gttggaccac acgtttgccg ccagcgcgaa tatcgcgatc
      421 ggcaaggccc gcaccgcggc actgttcaag aaatcgacga aggtattcga agacgcgatc
      481 aaaaacggcc gtacctcgat ggtgacgctc gggggcgacc ttcaaaactt cacacccctg
      541 caaggcggca ttccgttgga atgggagggc aaggtggtgg gagcaatcgg cgtcagcggc
      601 gcggccagtg cccagcagga tgaggagctc gcgatcgtcg gcgccgggtc gttgggctcg
      661 atggagatga cgggcaggaa caacgggcat gccccgttgt cggtcaccta catcgagagc
      721 cggagggtgt ccgcggcctt tgagaacggt gcggtattgg tcggtgaaga cgagaatatg
      781 atgcatgccg cccggaacta catggtgcat gccagccatc gcgataaggc cggtgtcgtc
      841 gagatccatg agttcgacac ggacattgtc tatgtcttga agggctcggc gcaggtcatc
      901 accggcggca caccggtcgg catgaagatg attgcgccgc atgaattccg cgcgccttcc
      961 gtggacgacg gcgagatgag gaggctcgtg ccgggcgatg tggtgattat tccgaacggg
     1021 gtgccgcact ggtttaagga agtggtggcg ccgtttgatt attacgtggt caaggtgcgg
     1081 tagacggatg ctcaaaacgg cccccaactt tgttctcggc tcgcaaccat cctcaacgca
     1141 ccctgagggt acgcctgccg tgcttgcatc gcctgcggcc tcgttggatg accgtgttga
     1201 gcatcctctg aggacggagc gtgaaatgaa caatcgaaga aacggaatga ttgtttttgc
     1261 agcgttcgcc ttcacgctct gtccaggttt tgcctggggg caggttaccg gtgatgtgcc
     1321 gggagtgcgg ccggaggcga ttgtcgattt gaaaaccgac gaaggactgg ccctggtcaa
     1381 gggccagtgg cggtacagca atgtcaaggt aatcgatgtc gatcatcacg gatctcgccc
     1441 cgtccggtcc tccgaatcgg acgcaagaca tcgacctgca tgccggcgcc gccgacttcg
     1501 acgattccaa gtgggaggtg ttgaagccga ttcaactgga agaacggcgt tcggccggac
     1561 ggctctgctt cagttggtac cgcaccagca tcaccatccc ggatagcatc ggtccattca
     1621 atcccgccgg ctccaccgtc gtttttgaga ttgccattga tgactatgcg gaggtgtggg
     1681 tggacggcag gttgccgctg gtcttggggc agcctggggg ccagttgatc aagggattca
     1741 atgcgccgaa ccgggtggta ttgactcgga atgccaaacc aggtcaaagg attcagcttg
     1801 cggtcttcgg catcaacggc ccgctctcga atccgccggg gaatttcatc tgggtgcgct
     1861 ccgctacact cgatttctac aggcccgatc acatcgggcc aacgcagttc gtcaagaccg
     1921 agatcctgaa gaccgatccg gcgctcgatc agatcgttgc cccgggcacg cagctggaac
     1981 aactcgctgg tggttttctc ttcaccgagg gaccggtctg ggtcccggcc accgccgatg
     2041 cttcgggata cctcctcttc agcgatccca acgccaatac gatctatcgt tggtcagcgg
     2101 aagggcaggt atccgtcttt cgcgccaaga gtggctacag cggcttcaat gtcggtgagt
     2161 accatcagcc gggctcgaac gggctcacgc tcgaccgtac gggacgtctt acgatcaacc
     2221 agcatggcaa tcgccgtgtc attcgcgtcg agccgcgcgg caacatcacg gtgttggccg
     2281 accggtatga cggcaagcga ctcaatagcc ccaacgatct cgtctatcgc tccgatgggg
     2341 cgctctactt caccgatccg ccgttcggct tgccgaaagt gttcgacgat cctcgcaagg
     2401 agctgcccca tagcggcgtc tactgcgtga aggacggcgt ggtgaaattg gtgagcaccg
     2461 agctcgatgc gcccaacggc atcgcgctgt ctccggacga gaagaccctc tacgtcaaca
     2521 actggaacga caaacggaag gtgatcgtac ggtacgacgt caattcggat tgcacgctct
     2581 cgaacagccg gctgttcttc gatatgacca atgcgccggg atccgacgcc ctcgatgggc
     2641 tcaaggtcga tctgcagggg aacgtctatt caactggacc aggcgggctc tggatcctct
     2701 cgccggatgg caggcaactc ggcctcatca aggggccgga ggatccgcac aacatggcct
     2761 ggggcgacga tgacggcaag accctctaca tcacggcgct gacagggatc tatcgcatca
     2821 ggctgaatat tccgggggtg cggccatgag tcacctggcg ggcaggggga ttctgttggc
     2881 gcttctcgtc ctgctggttc atgtcgcggc atgggctgag ccagtcccgg tcacgattac
     2941 tagtctcgat ccgcgtttcg atcagcttgt gccgaagaat acgcaggtcg aaaaaatcgc
     3001 cgatgggttc acctgggtcg aagggccggt ttggcacaaa cagggcggct atctgctctt
     3061 ttccgacatt ccggccaacc gcgtctatca atggaaaccg gggaagggca caagcgtcta
     3121 tctcaaaaac agtggctata gcggctcgac tccgttcgcc ggcaaggagc caggctcaaa
     3181 tggactgacg tttagtgccg acggacaact gtacctctgc cgccatgggg atcgtcaaat
     3241 tggccgtttg gaagcggacg gccgtatcac gccggtcgcc gctcgcttcg atggtcacct
     3301 gatcaacagc cccaacgacc tcgtcttcaa gtccaacggc gatctctatt tcaccgatcc
     3361 gccgttcgga cttcctggcg tcttcgacga ctccggcaag gctccggtgc aaggggtcta
     3421 tcgcctctct cacgatggag cgctcacgct gctcatcaat gacatcaagg ctccgaacgg
     3481 gattgccttt tcgccggatg agaagaccct ctacgtgtcc gatgtcgatc cgaagcgtgc
     3541 ggcctggcta gcctacgatg tgaaagccga cggcactgtc gccgacggcc gtgtgttctt
     3601 tgacgcgatg cgttggagaa aagatccgtt ctttgggccg gacggattca aggtcgaccg
     3661 gaaagggaat atcttcggcg cccgaccggg tgggatcagc gtgatcgcgt cagacggcac
     3721 gctcctgggc acgatcgaga ccaggcaacc gatgtccaac gtcgcatggg gagaagacgg
     3781 gcagaccttg ttcatgactg gtggttcatc gatctatcgt cttcgcgtga ctacaggcgc
     3841 ggaccgctac tagcctcctg taagactttc cgccgccggg cgactctcgt tcagtatcgg
     3901 ttcaacctaa ggagtcatat ggcctcacat cgtcggattc cttccgctcc accgtcgccg
     3961 attccggcag aacagcagcg tcttgacgaa gacgcgcagc gccggcgcca ctggaaacgc
     4021 tggggaccct atttgagcga gcgcgcctgg ggaacggtgc gtgaggacta cagcccgtat
     4081 ggaacagcct gggacgcgtt cccccatgat caggcccggt cgcgtgccta tcgctggaac
     4141 gaggacggcc tcgccggcat ttgcgatcgc catcagatca tctgctttgc cctggccttg
     4201 tggaacgggc gagacccgat cttgaaagaa cgagtcttcg gactcaccgg caacgaaggc
     4261 aatcacgggg aggatgtgaa ggagtactat ttctacctcg attcgacgcc gacgcattcc
     4321 tacatgaaat atctgtacaa gtacccgcag gcagcgttcc cctataccag gctggtcgag
     4381 gagaatcgcc gccgagggcg ccatgatccg gagttcgaac tgatcgacag cggtgtcttc
     4441 gacgagaacc ggtatttcga cgtgtgcgtc gagtatgcca aaacgacgcc ggaagatctg
     4501 tgcatccgta ttcaggtcgt gaatcacggg ccggaggagg ctcaactcac gctgcttccg
     4561 acaatctggt ttcgcaacac ctggtcctgg ggcacggaca tccggcgtcc tcgtttcagg
     4621 caaggggagt cgatcgacca catgagagtc atcgagacgc agcacgagca ttatggtctg
     4681 cgccggttgc tctgcgaagg cgaacccacc ctgctcttta ccgaaaatga caccaacagc
     4741 cgccgtctct atggtgatca aacaggcccc cggtacgtga aggacagctt tcacgactat
     4801 gtcgtgcagg gtgagaa
//
LOCUS       DAORQN010000111         4796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_694_length_4796_cov_10.421852, whole genome shotgun sequence.
ACCESSION   DAORQN010000111 DAORQN010000000
VERSION     DAORQN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_694_length_4796_cov_10.421852"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..2433)
                     /locus_tag="PK614_06765"
     CDS             complement(34..2433)
                     /locus_tag="PK614_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRB81950.1"
                     /translation="MTSPTTPTISSAAQQRIVDLIAKELGVTAPQIAAAVTLLDGGAT
                     VPFIARYRKEATNNLDDTHLRTLEERLLYLRELEERRQTILASIDEQGKLTDELRRAV
                     EQAATKQAVEDIYLPYKPKRRTKAQIAREAGLEPLANALFADPTLDPDQEAAKYVKVV
                     AAAEGVEAVNVPDAKAALEGARDILVERFAETAELLATLRTKLWNEGIVTSTVMPGKE
                     TAEEEKFRDYYAYSETIRTIPSHRALAMFRGRTLGVLKLDLGLGESLEAIVPHPCAAM
                     IAAHFGIENRGHRADKWLTDVCYWAWRVKVHLHLSTELLLQVREAAEAEAIKIFGRNL
                     HELLLAAPAGPKAVLGLDPGLRTGCKVAVVDATGKLLDTATIYPHQPRNDWQGALATI
                     TQLVLRHGVELISIGNGTASRETDKFAAEVVKLVAEQKPEQKLAKIVVSEAGASVYSA
                     SAFAAAEFPSLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVNQRALARSL
                     DATVEDCVNAVGVDVNTASAPLLARVSGLNRVLAQNLVEYRDTHGRFQNRHMILKVPR
                     LGEKTFEQAAGFLRINDGDNPLDRSAVHPEAYPVVERMLARLNKGIAEVMGKPAALKE
                     LSPAEFTDETFGLPTVRDILTELEKPGRDPRPEFKTATFRDGVESLADLQAGMVLEGV
                     VTNVAAFGAFVDIGVHQDGLVHVSALANKFVKDPHEVVKPGQIVKVKVLAVDVPRQRI
                     SLTMRMEDAATPASQPDPRAGGPRDTRDRRPADQQRGGSREPQPIGAFALALARAKEK
                     K"
     gene            2757..4640
                     /locus_tag="PK614_06770"
     CDS             2757..4640
                     /locus_tag="PK614_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRB81951.1"
                     /translation="MPKHLNNQSSSVSLTVAAMGVVYGDIGTSPLYALRECFHESHGL
                     PVTPDNVLGILSMIVWSLVLVVTVKYLLFVMKADNEGEGGMLALLALSQQSRPVDLRK
                     GLNVVVTLGLIGVAFLYSDGIITPAISVLSAVEGLTLTTDVFTPYILPLTVALVALLF
                     MIQRRGSGTIGSIFGPIMLLWFVTMALLGLHSLIQTPEVLRAANPLYAIQFLIRHAGV
                     GILVLGSVFLVLTGAEALYADMGHFGRRPIQLGWFVVALPALLLQYFGQGALLMRTPA
                     ALANPFYFLAPDWMLYPLIALATMATVIASQAMLSGAFSLTLQAVQLGYLPPLRITHT
                     SAEQHGQIYFGLLNWLMFIGTVGLILSFGSSSNLAAAYGIAVSGSMIITTLLMYRVVQ
                     AHWQWSPLPAAMAVGGFLLIDLAFFLANAQKIPHGGWFPLLLGAAVFTVMSTWARGRA
                     IVADHLREQFPPLLQFAREVLSQVQCRTAGRAVFLSQHPDITPPALLQNVRHNKSLHE
                     QVYILTVRTEPVPYVPSGGPLEITTIQENLFQVVARCGFMETPDIPRVLTYLAARGHT
                     LPVEETTFFLSRLTFLATPKPGMAIWREKIFVFLSRNTQRASSYFHLPAEQVVEIGLV
                     LEI"
BASE COUNT          859 a         1544 c         1372 g         1021 t
ORIGIN      
        1 tcaccagcgc gccggaggcg agcaggccgc tcgctacttt ttctccttgg cgcgggccag
       61 ggccagtgca aatgccccga tgggctgagg ctcacgggat cccccccgct gctgatcggc
      121 ggggcgtcgg tcgcgcgtgt cacgcggccc tccggcacgg ggatcaggct gtgaggccgg
      181 cgtcgcggca tcctccatac gcatggtcag cgaaatccgc tgtcgcggta catcgacggc
      241 caacaccttc accttcacga tctggcccgg tttcacgact tcatgcggat ctttgacgaa
      301 cttgttcgcc agcgcagaca catgcacgag gccgtcctga tgcacgccga tatcgacgaa
      361 tgcgccgaag gccgcgacgt tagtcacgac tccttcgagc accatcccgg cctgcagatc
      421 ggcaagcgat tcgacgccgt cccggaaagt ggccgtcttg aactccggac gcggatcacg
      481 gcctggtttt tccagctccg tgaggatatc gcgcaccgtc ggcagaccga aggtctcgtc
      541 ggtgaattcc gccggcgaaa gttctttcaa tgcggccggc ttacccatga cttcggcaat
      601 gcctttattc agccgcgcca gcatgcgctc gaccaccggg taggcttccg ggtggacggc
      661 ggaacgatcc agcggattgt cgccgtcgtt gatccgcagg aaccccgccg cctgctcgaa
      721 tgttttttcg ccgaggcgcg gtaccttgag gatcatgtgc cggttctgga accgaccgtg
      781 ggtgtcacgg tactccacga ggttttgcgc cagcacgcga ttcaagccgg ataccctcgc
      841 cagcaacgga gccgacgcgg tgttcacgtc cacgcccacc gcattcacgc agtcttcaac
      901 cgtcgcatcg agcgatcggg ccagggcccg ctgattgaca tcgtgttggt actggcccac
      961 gccgattgat ttgggatcga tcttgaccag ttcggccaac ggatcctgca accgtctggc
     1021 aatcgatacg gcgccgcgta gactcacatc cagcgatgga aactccgcgg cggcaaaggc
     1081 cgaggcggaa tagactgagg cgccggcttc acttaccaca atcttcgcca gtttctgttc
     1141 cggtttctgc tccgccacca gcttgacgac ttcggcggca aatttgtcgg tctcccggct
     1201 tgcggtacca ttgccgatgg aaatcaattc gacaccgtgc cgaagcacca actgggtgat
     1261 cgtcgccaag gccccctgcc aatcgttacg cggttgatgc gggtagatcg tcgccgtgtc
     1321 cagcaatttc ccggtcgcat ccacaaccgc cactttacag ccggtgcgga gaccaggatc
     1381 gagaccgagc acggccttcg gaccggcggg cgccgccagc agcagctcat ggaggttacg
     1441 accaaaaatc ttgattgcct ccgcttcggc ggcttcgcgc acctgcagca acagctcggt
     1501 gctcagatgc agatgcactt tcacgcgcca ggcccaatag cagacatcgg tcagccattt
     1561 gtcggcacgg tgcccgcgat tctcaatacc gaagtgggcg gcaatcatcg ccgcgcaggg
     1621 atgcggcacg atggcttcga gactttcccc taacccgagg tcgagcttca acacacccaa
     1681 cgtgcggccg cgaaacatcg ccagggcacg atgcgaggga atggtacgga tggtttctga
     1741 ataggcgtag taatcgcgaa acttttcttc ttccgccgtt tccttgccgg gcatgaccgt
     1801 ggaggtcaca atgccttcgt tccacaattt tgtgcgtagc gtcgcgagca gctcagcggt
     1861 ttcagcgaat cgttcgacca gaatatcgcg ggcgccctcg agcgccgcct tcgcgtcggg
     1921 tacgttgacg gcttcgacgc cttccgctgc agctaccacc ttgacatact tggcggcttc
     1981 ctgatccgga tcgagcgtcg ggtcggcgaa gagcgcgttc gccaatggct ccagtccggc
     2041 ctcgcgcgca atctgcgcct tggtgcgacg tttgggtttg tagggcagat agatatcttc
     2101 cacggcttgt ttcgtcgcag cctgctcgac cgcccggcgc agctcgtcgg tcaatttccc
     2161 ctgctcgtcg atcgacgcga gaatcgtctg ccgccgctct tccaattcac ggagatacaa
     2221 gaggcgctct tccagggtgc gcaggtgcgt gtcgtccaag ttgttggtag cctctttgcg
     2281 ataacgcgct ataaagggca ccgtcgcgcc accgtccagc aacgtcaccg cggccgcaat
     2341 ctgcggggcg gttacgccaa gttccttggc gatgagatcg acgatgcgtt gctgggctgc
     2401 ggatgaaatg gtcggtgtcg ttggagaagt cataggtgcg tattgggctt tccgaaagcg
     2461 agatccggcc ggaggcgacc ctcccggtct cagcggcttt cttgtgagat gaattcgaag
     2521 acttggccgg cgcgactcct atgatcgttc gcacgggccg acggggaatc tagcagaaga
     2581 ttcgcgcaac ttccaggccg gatgcgcggc cactcacctc tcttcacatg ggagcagatg
     2641 cggcggttta cgacaccctg cggaaccaac cggcatcccc tcatccaaca tggtatagtt
     2701 cgacccgtca caacacagga ccatcgcgaa tcgggtcggg cccccgtcac gtatccatgc
     2761 cgaagcatct caacaaccaa tcttcttccg tctccctcac cgtggccgcc atgggagtgg
     2821 tctatggcga catcggaacc agcccgctgt atgccctgcg cgagtgtttt cacgaatcgc
     2881 atggcctgcc ggtcactccc gacaacgtgc tggggattct gtccatgatc gtgtggtcgc
     2941 tcgtgctcgt cgtcaccgtg aaatatctgc tgttcgtaat gaaagccgac aacgagggcg
     3001 agggaggcat gctggcgctc ctggcgctca gccagcagtc ccgcccggtc gatctccgca
     3061 aagggctcaa cgttgtcgtc acactcggct tgatcggcgt ggcgtttctc tatagcgacg
     3121 gcatcattac accggccatt tcggtcctta gcgcggtcga agggctcacg ctgaccaccg
     3181 acgtgtttac cccctacatc ctgcctctga ccgtcgcgct ggtcgccttg ttgttcatga
     3241 tccaacgccg gggctccgga acgatcggga gcatcttcgg ccccatcatg ctgctctggt
     3301 ttgtcacgat ggccctgctc gggctccaca gtttgatcca aaccccggag gttctgcgtg
     3361 ccgccaatcc gctgtatgcg atccagtttt tgattcggca tgcaggagtc ggcatcctgg
     3421 tgctgggcag tgtgtttctg gtcctcacgg gtgccgaagc gctgtatgcc gacatgggcc
     3481 acttcggacg ccgaccgatc caactcggct ggtttgtggt ggccttgccc gcccttctgc
     3541 tccagtactt cggccagggt gcgctcctca tgcggacccc ggcggctctg gccaatccct
     3601 tttacttcct ggcccccgac tggatgctgt accccttgat cgcgctggcg accatggcca
     3661 ccgtcatcgc atcccaagcc atgctgtccg gggccttctc gttgaccttg caagccgttc
     3721 aattgggcta tctgccgccg ttgcgaatca cccacacgtc cgccgagcaa catggccaaa
     3781 tctactttgg cctgttgaat tggctcatgt tcatcggcac cgtcggtctg atcctttcct
     3841 tcggatcgtc cagtaatctg gccgcagcct atggcattgc cgtctcgggt tccatgatca
     3901 tcacgaccct cctgatgtac cgtgtggtcc aggcccattg gcaatggagc ccgctaccgg
     3961 cagccatggc agtcgggggc ttcctgctca tcgatctcgc cttctttctc gcgaatgccc
     4021 aaaaaattcc ccatggcgga tggttcccgc tgctgctcgg cgcggccgtg ttcaccgtca
     4081 tgagtacctg ggcgcgagga cgcgcgatcg tggcagacca cttgcgcgag cagtttcctc
     4141 cattgcttca attcgcccgg gaagtcttgt cgcaggtgca atgcagaacc gccgggcgcg
     4201 ccgtattttt atctcaacat cccgacatca ccccgcccgc cttgctgcaa aatgtccggc
     4261 acaacaaatc tctgcacgag caggtctata tcctcacagt ccgcacagag cctgtgccct
     4321 acgttccatc cggaggcccg ctggaaatca ccaccatcca ggagaacctg tttcaagttg
     4381 tcgccagatg cggattcatg gaaacgccgg acattccacg tgtactcacc tacctcgcag
     4441 cccgcggcca taccctccct gtcgaggaga ccacgttctt cctgagcaga ctgacctttt
     4501 tggcgacgcc gaaaccgggg atggcaatct ggcgggaaaa aatatttgtc ttcctgtcac
     4561 gaaataccca gagagccagt tcctatttcc acctaccggc ggaacaggtg gttgaaatcg
     4621 gactggtgct ggagatttga tcgcgtcgct gagcggtctg tgacgagccc tgcccggtgg
     4681 gatccggcgg tgcctgcgaa ggaatgaccg gcgatcagac acctttccca aattcgacct
     4741 gccgccaggc ttcgtagagc accaccgcca ccgagttgga gaggttgagg ctgcgg
//
LOCUS       DAORQN010000112         4780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_697_length_4780_cov_7.368677, whole genome shotgun sequence.
ACCESSION   DAORQN010000112 DAORQN010000000
VERSION     DAORQN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_4780_cov_7.368677"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PK614_06775"
     CDS             <1..1853
                     /locus_tag="PK614_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRB81952.1"
                     /translation="EQGFLDPDVREWFMTTGTLHILSISGSHLGLIALLSFALVRKAC
                     LLLPSILLLGLSRWLTATRLAALLTLGPVLAYTVLAGAETATIRSAIMIAVALWTVWL
                     GSPHYLLHALAAAAGLTLVVNPVALFDISFQLSYVSVLALALALSRETPVDELPLPQE
                     STAARIVYWFRESLRLTALVTLATLPLVACYFNQVSWMGLFANLLMVPFVGFIFLPIS
                     LLSVVWVIATDSSTLPGAAVIDSLGQGLITSTQVLAAFPGAEWFVAAPTIPMMLLFYL
                     LGWALLAGWPAHATPLLRRSMASGLACILLWWLWSPRPFGGEEMVRVTFLDVGQGDSA
                     VVELPDGTVTLIDGGATYERFDMGRSVVAPFLWNRGIRSIDHVIGTHPQLDHVGGLAW
                     ILAHFQVEKFWTNGAARQEEFWRRIEQAMVQRKLEATVATESRLISDGPACRMVVLNP
                     WPSPVSTNSTKGESLNNRSVVTELVCREQRVLFTGDIERETLTRLTDSGTSAHIALLK
                     VPHHGAKSSLERRWLDTIRPAVAVVSAGRRNPYGHPAGDVLAAYEAVGTQLWRTDRDG
                     AIWADLDLRRQQLSIHSTREWILQPALPSADMWSIEQDNLRRLWRRWNWM"
     gene            2259..3440
                     /locus_tag="PK614_06780"
     CDS             2259..3440
                     /locus_tag="PK614_06780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB81953.1"
                     /translation="MTKRLLFLAPAPPSDRQGGGALRMLHLLRFLGSRFQVDLIAPAL
                     DGWEDAQRLLRDVCAEMMFVPPQGPGFLDRIGQVSPYVKDRALAAVVHEHLASGQYGA
                     VQLEKPAMIPYLPSQVSVPVVLDVWGYSSTSPLRILRGRGGASGRSRQLVRLIKVGIF
                     DRFRWPMTHCVTVVSEEDRIRCERAHPGQRVLVVPNGVDCRTILPKPDHAATSPLLLF
                     TGDMGAEPTVEAALFLATEVFPAIRRDFPKAELRLVGRNPDPRVRRLAGSGIEVTGAV
                     PDMQPHLRAATMYVAPIFTGTGTRTRLLEAMAAGLPIITTSVGIEGMKVQPGRDLLVA
                     DQPVDMIESIHTLLMSQPDRERFGHAARHMAEIWYDWSRCLWPLEPLYQNILIPKAVA
                     C"
     gene            3625..4692
                     /locus_tag="PK614_06785"
     CDS             3625..4692
                     /locus_tag="PK614_06785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB81954.1"
                     /translation="MTLIPHSKPSLGQEDIRAATEVLRSGRIAGGPVVGQFERGMAAY
                     LGVLGGVAVSSGTVALELALRAMGVGHGDNVILPSYVCSAPWLAVQRVGAQARIVDID
                     PVTYNLDPHKVRKARTSRTRAVIVPHLFGLPADLTVLQSLGIPLIEDCAQTLGTTEQG
                     RAVGSVGLLTVCSFYATKLLCTGEGGMVLSNDEALLERVRALREYDQAPSLNAAAFNC
                     KMTDLQAAIGVTQLNQLGEFLEKRAVLAAVYRDSLPAELFTPPSVPTGRTHAYYRFVV
                     RLQKGLQSSDEFTDYLSRMAHRGLQCRKPVFRPLHRYLELADFPASDDADAVAISLPL
                     YPSLTEDEVKLAAQILQEELR"
BASE COUNT          816 a         1386 c         1526 g         1052 t
ORIGIN      
        1 gcgaacaggg gttccttgac ccggacgttc gggaatggtt tatgacgacc gggacgttgc
       61 acattctttc gatctccggt tcacatctcg gtctcatcgc cttgctctca ttcgcattgg
      121 tcaggaaggc gtgcctgttg ctgccgtcca tacttctgct gggcctctcg cggtggctca
      181 cggcgacgag actggccgcg ttgctgacac tggggccggt gctggcctat acggtgctgg
      241 ccggcgcgga gaccgccacg attcgtagtg ccatcatgat cgccgttgcg ctctggacgg
      301 tatggctcgg ctcaccgcat tatctcctgc acgcacttgc tgccgccgcc ggtctgacgc
      361 tggtggtcaa tcctgtggcg ttgttcgaca tttcctttca actgtcctac gtctcggtct
      421 tggcgctggc tctggcactg tcgcgcgaga cgcctgtcga tgaattgccg cttccgcagg
      481 agtccaccgc cgcacggata gtgtactggt ttcgcgagtc gctgcgactg acggcgttgg
      541 tgacgctggc caccctgcct ctcgtggcct gttatttcaa tcaagtgtca tggatgggct
      601 tgttcgcgaa tcttctcatg gtgcccttcg tcggtttcat cttcttgccg atcagtttgc
      661 tgtcggtcgt ctgggtgata gcgaccgaca gcagcacgct ccctggcgct gcagtcatcg
      721 attcgttggg gcaagggctg attacttcga cccaggtcct ggctgctttt ccgggcgcgg
      781 aatggttcgt ggccgcaccg acgattccca tgatgctgct gttttatctg ctggggtggg
      841 cgctgttggc gggttggccg gcccatgcga cgccgctgct cagaaggtct atggcctctg
      901 gccttgcttg tatcctgctc tggtggctct ggtccccacg tccattcggt ggggaggaga
      961 tggtgcgcgt gaccttcctc gacgtggggc agggtgatag tgcggtcgtt gaattgcctg
     1021 atgggacggt cacgctgatc gatggcggag cgacctatga gcggtttgac atggggcgga
     1081 gtgtggtggc cccttttctg tggaatcgag ggattcgaag catcgatcat gtcatcggga
     1141 cacacccgca attggatcac gtcggaggcc tggcttggat cctggctcat tttcaggtag
     1201 agaaattctg gaccaacggg gcggcgcggc aggaagaatt ctggcgcagg atcgagcaag
     1261 cgatggtgca gcggaagctc gaagccacgg tggcgacgga aagtcggttg atttcagatg
     1321 gacctgcctg tcgtatggtg gtgctcaacc catggccaag cccggtctcg acgaattcca
     1381 caaaaggcga gtcgctgaat aatcgttcgg tcgtcactga attggtctgt cgcgagcagc
     1441 gtgtcctgtt tacgggagat atcgaacggg aaactttgac ccgtctcacc gattccggga
     1501 cttccgctca cattgccctc ttgaaagtcc cgcatcatgg ggcgaagagt tcgctggagc
     1561 gccgatggct tgacacgatc aggccggccg tcgcggtcgt atccgccggt cggcgcaatc
     1621 cctatggtca tccggccgga gacgtcctgg cggcctatga ggcggtgggg acacaactgt
     1681 ggagaaccga ccgggatggc gccatctggg ccgatctcga cctccgtcga cagcaactgt
     1741 cgatacacag caccagggaa tggatactac aacctgcgct gccctcggcg gatatgtggt
     1801 ctatcgagca ggataatctg cgccgcttat ggcgccgctg gaattggatg taacccgcac
     1861 gagcctctcc tcatctcaac gccgtgaccg aagatcgtcg catcactcaa cccggacctt
     1921 cctgtgcaga tatagccgaa tcgagtggca gcgggctgtc aattttgccg acaggtcaga
     1981 aacgcccact gcataagtcg tcaatattgc gtaagagtgg tggatcacct gcaaggcata
     2041 gcacatgcag gcattcttca ccgtccggca actctgtccc ggcagaccga aaggcattgg
     2101 ttccgtcctt cagttcaggc gaacgtgaag cctcgacgtg atgcctcgag gcagtcggac
     2161 tacagggctc ggagcattct cggcgcagtt gccggccggc ggtacaatca ggttggggga
     2221 ttgatcccga tgagtgcgga agaacaagcg gggagaccat gacgaagcgt ctcttgtttc
     2281 tggctcctgc gccgccctcc gaccggcagg gcgggggagc cttgcgcatg ctgcacctgc
     2341 tacggttcct ggggagtcgc tttcaggtgg atttgattgc tccggcgctc gacggctggg
     2401 aggatgcgca acgactgtta agggatgtct gcgcggagat gatgtttgtt ccgccgcaag
     2461 ggcccggttt tctcgatcga atcggtcagg tgagtcccta tgtcaaagat cgagcgttgg
     2521 ctgccgtcgt ccatgaacac ctggcgagcg gccaatacgg ggccgtgcag ttggaaaagc
     2581 ccgcgatgat tccctatctc ccgtcccagg tgtcggtgcc ggtagtgttg gatgtctggg
     2641 gttatagctc gacaagtcca ttgcggatac tcaggggccg aggcggagct tccggtcggt
     2701 cgagacaact ggtccggctc atcaaggtgg ggatcttcga ccggttccgc tggcccatga
     2761 cacattgtgt gacggtcgta tcggaggagg atcgaatccg ttgcgaacgt gcccacccgg
     2821 gccaacgggt gctggtggtt cccaacggcg tcgattgccg gacgatcctg cccaagccgg
     2881 atcatgccgc tacgtcgcct ctgttgctgt tcacaggtga tatgggggcg gaaccgactg
     2941 tcgaggccgc gctcttcctg gccacggaag tgtttcctgc cattcggcgg gatttcccca
     3001 aggcggaatt gcgattggtc gggaggaacc cggacccccg tgtgcgccgc ttggcgggct
     3061 ccggcatcga ggtgaccgga gcggttccgg acatgcaacc ccacctgcgc gcggctacga
     3121 tgtatgtcgc gccgattttt accggaacgg ggacgcgcac tagactgctg gaggccatgg
     3181 cggcagggct cccgatcatc accacgtcag tcgggatcga agggatgaag gtacaaccgg
     3241 ggagggattt gttggtggcc gatcagcccg tcgacatgat tgagtcgatt cacaccttgc
     3301 tgatgagcca gcccgatcgc gagcgcttcg gccatgcggc tcgccatatg gcggagatct
     3361 ggtatgactg gagccgttgc ctttggccgt tggagccgct ctaccagaat attctgattc
     3421 cgaaggccgt ggcctgttga gcgctgcctc cggccggctg cgaatcacgt tcggtgacat
     3481 ctcaacagtc tcgttgcacg caccgttcgg cgtggtagca gaggaatctg ttgtgttgta
     3541 ccggcccctc cacgtagaat ttcccgccga cacgattccg ggctgctccc tgctcacccg
     3601 gcatgacaag cggttcctga cgccatgacg ctgattccgc attcaaaacc gtccctggga
     3661 caagaggaca ttcgtgcggc gaccgaggtg ctccgatccg gtcggatcgc cgggggaccc
     3721 gtcgtcgggc aattcgagcg tggcatggcc gcgtatctcg gagtgctggg cggggtggcg
     3781 gtcagctcag gtacggtggc cctggaattg gcgttgcgtg ccatgggcgt cggacatggc
     3841 gacaatgtca tcctgcccag ttatgtctgt tcggcgccat ggctggcggt ccagcgagtc
     3901 ggcgcacaag ccaggatcgt ggatatcgat ccggtgacct acaatctgga tccacataaa
     3961 gtccgcaagg cccgcacgtc acgcactcgg gcggtgatcg tgccgcacct gttcgggtta
     4021 ccggccgatc tgacggtgtt gcaatccctt gggattccgc tgatcgaaga ttgtgcgcag
     4081 accctcggta ccacagagca gggccgcgcg gtgggcagtg tcggcctgct cacggtctgt
     4141 tcgttctatg ccacgaaact gctctgcacc ggcgagggcg gcatggtgct gtcgaacgat
     4201 gaggcgctcc tggaacgggt tcgggccctt cgggagtacg atcaagcgcc gtcactcaat
     4261 gcggcggcgt tcaactgcaa gatgacggat ctgcaagccg cgatcggcgt cacgcagctc
     4321 aaccagctgg gagaatttct cgagaagcgg gcggtgttgg ccgcggtcta tcgtgacagc
     4381 ctgcctgccg aactcttcac tccgccctcg gtgccgacag gacgaaccca tgcctactat
     4441 cgatttgtcg tgcggctgca gaaaggattg cagtcgtccg atgaattcac ggactatctc
     4501 tcgcgcatgg cgcatcgagg tctgcagtgc cgcaagcccg tcttccggcc cttgcatcgg
     4561 tatctcgaat tggctgattt ccctgccagt gacgacgccg acgccgtcgc gatttcgctg
     4621 cccctctatc cgtcgttgac cgaggacgag gtgaagctgg cagcgcagat cctgcaggaa
     4681 gagcttcggt aacgccgcct tcgtattgat cgctttctcg atcgacccgc gtggtcccgc
     4741 accgcgtatg ggtgggcatc ctgccatcat acccttgacc
//
LOCUS       DAORQN010000113         4742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_705_length_4742_cov_6.823555, whole genome shotgun sequence.
ACCESSION   DAORQN010000113 DAORQN010000000
VERSION     DAORQN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_4742_cov_6.823555"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..519
                     /locus_tag="PK614_06790"
     CDS             160..519
                     /locus_tag="PK614_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HRB81955.1"
                     /translation="MIRSLQPCPSSPNCVSTQASDQGHGILPYRYQKTLAEAKETLKT
                     IVANLPRVKLVEEDEAYLHYEFTSLLLRFVDDVEFVFDDATKTIHFRSASRTGYSDLG
                     VNRRRMEEIRVVVEGRL"
     gene            688..1233
                     /locus_tag="PK614_06795"
     CDS             688..1233
                     /locus_tag="PK614_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81956.1"
                     /translation="MNIRNRAMIKQASRLALVLVLGLSSACVSVQVDPLTSKAFEPRP
                     GSEDVTTLQREPNHGHVQIARIIATSEYASEDTLRDRILARAKELGADAVVLGDADVR
                     RLADRGPTFQSTMGTAVPGATSGNSYRSGYWNPFRMDAWSFTQGAGGGQGWMLHMSGL
                     AIRYVSADELRAAPKPVPPAP"
     gene            1610..>2362
                     /locus_tag="PK614_06800"
     CDS             1610..>2362
                     /locus_tag="PK614_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB81957.1"
                     /translation="MSALTVDQRPSSTLPHVRIQPARGLLDLDLAALWQYRELIYILV
                     WRDVTVRYKQTLLGVAWSMFQPVATMLIFTVVFSVMGKIPSDGFPYPVFLYAGLLPWF
                     YVSQALSRGGTSLVGEAPLISKVYFPRLILPLSATIAPLVDLVLAFVAFLGLMAWFGI
                     LPTWRILSLPLFAALAATITLAAGLWISALYVKYRDVGHILPMFIQLWMFVSPILYPV
                     SKVPESWRALYALNPVVSVVEGFRWALIPGFQV"
     assembly_gap    2363..2372
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2468..3769
                     /locus_tag="PK614_06805"
     CDS             2468..3769
                     /locus_tag="PK614_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB81958.1"
                     /translation="MSDIAIKADCLSKHYRLGAASRQHNTLRDHLMHRLRGLTRWGGG
                     PPESNPSFWALKDVSFEVKRGEVLGIVGHNGAGKSTLLKILSRITQPTTGTADIYGRV
                     SSLLEVGTGFHSELSGRENIYLNAAMLGMRRGEVRRKFDEIVAFSGVEAFIDTPVKRY
                     SSGMYVRLAFAVAAHLEPEILIVDEVLAVGDASFQQKCLGKMEEVSRSGRTVLIVSHN
                     MTVIEGLCERAILLEKGRVAKIGNTHEVVEGYADAIRGLAGTAIEARHDREGLGEILL
                     TRIEVMDTQRHPVAAAITGRDVVIRMYYRCAAGKEFKNCRVSISVNGRKAQDLFVLST
                     DIVDPKPLTLRGEGYVDFILPELPLTGGAYFFQSYIESNGHAQDWIKNVAPFSVLDAD
                     FYGTGKLCPPGWEANGVLVRYRWEAGETGSTGALPIRARAS"
     gene            3921..4124
                     /locus_tag="PK614_06810"
     CDS             3921..4124
                     /locus_tag="PK614_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81959.1"
                     /translation="MGSGFEDGQQAQLELAGLRRSLKWTNIQREQLLDRLDLLRLDNQ
                     RLQERVDELERQLAEFKQQQALF"
     gene            4431..>4742
                     /locus_tag="PK614_06815"
     CDS             4431..>4742
                     /locus_tag="PK614_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81960.1"
                     /translation="MTHSIKTVDDLRWFLSRTAGFRGGHITDVHLSKRRIFDEASGRE
                     VMAGSMATVVVRYHAQGILRVAKLTMLGVTDLSLFEQDGSDCSSLGAIQVEMSEGKLR
                     FW"
BASE COUNT          897 a         1273 c         1456 g         1106 t
ORIGIN      
        1 cccccccggc cgcgcggccg cccgcccccc cgccccagcg acgacctcgt gcaacgcgcc
       61 ccccccctcc cggccccggc cggcgggggg cgtgaatcct gtcaacggtc tccggcttgg
      121 agcccgtacc cgtaggctct atatattgtt gataacacta tgatcagatc actccagccc
      181 tgcccatcca gcccgaattg tgtgtcgacc caggcatccg atcaaggcca tggcattctg
      241 ccgtatcgat accagaaaac tctggcggag gctaaagaga cgctcaaaac cattgtggcc
      301 aatctgcccc gtgtgaaact cgttgaggag gacgaggcct acctccatta cgaatttacg
      361 agcctgctgc ttcgattcgt ggacgatgtc gaattcgtgt ttgatgatgc gacaaagacg
      421 atccattttc ggtcggcttc ccggacgggc tatagcgatc tgggtgtgaa tcgacggcgt
      481 atggaggaga ttcgagtggt cgtcgaaggc aggttgtagt gcaaagcatg acgctgaaga
      541 ataggataca agagtttccg gattgaccgc acagtcggcg atgggtacca tcaagtctcg
      601 gagcaatttg gggttgaatt gatcgatcca agccgtcact atagaactcg atcaagctaa
      661 agtagatctg accctggact gttggatatg aacattcgaa atcgagccat gataaagcag
      721 gcctctcgcc ttgcgttggt ccttgttctc gggttgtcga gcgcctgtgt ctccgtgcag
      781 gtggatccct tgaccagcaa ggcgttcgag cctcgacctg gaagcgagga cgtgacgacg
      841 ttacagcgcg agcccaatca tggacacgtg cagatcgccc gcatcattgc gaccagtgag
      901 tatgccagcg aagacacgtt gcgtgatcgg attctcgccc gggcgaaaga gttgggggcc
      961 gatgctgttg tccttggaga tgcggacgtg cggcgcctcg cagatcgagg cccgacattc
     1021 caatcgacca tgggcacggc ggtaccggga gcgacgtcgg gtaattcgta tcgcagcggc
     1081 tattggaatc cgtttcgaat ggatgcgtgg agttttacgc agggagccgg tggtgggcag
     1141 ggctggatgc tccacatgtc cgggttagcg attcggtatg tctctgccga cgaactgcgt
     1201 gccgctccaa agcctgtccc tcctgcaccg tagccgatcg tttctattac acgtgactgc
     1261 tcccaaccct gtatccttcc tcatccagag tcatttggta tagttgattc ttcaaggtat
     1321 tgcctggcaa ttgaggtgat ggagttttcc tggtgggcgt cctgcaattt ccgcctgctc
     1381 gccgtggggc cgggattcgg cgatccatgc agtgctgggg ccccaaaggg gcataacagt
     1441 tcgaatttct tgactaaagc ggaaaatttt gacataaacg attacagtcg gttcgagtta
     1501 gaaccaagtg taatgtggct gtgaggccct cttgtttttg aggcgcgggg gccggtcttt
     1561 cgatacggtt gccgtcggtg tgaggatcat gaggagtgac gaaattgcca tgtccgcgtt
     1621 gactgtcgac cagaggccat cgtcgacctt accgcacgtg cgtatccagc ctgcccgcgg
     1681 cctattggat ttggatttgg ccgcgctctg gcagtatcgc gagctgattt atattcttgt
     1741 ctggcgtgat gtgaccgtgc ggtacaagca aaccctgctg ggagtcgcct ggtcgatgtt
     1801 tcagccggtc gcgaccatgc tcatttttac ggttgtattc agtgtcatgg gaaaaattcc
     1861 atccgacgga tttccctacc cggtgttcct ctacgcaggc ctgctgccgt ggttctatgt
     1921 gtctcaggcg ttgagcaggg gaggaaccag tttagtggga gaagccccgc tcatcagcaa
     1981 agtgtatttc ccacgcttga tcctgcccct ttcggccacc atcgcgcccc tggtcgatct
     2041 ggtcttggcc ttcgtcgcgt ttctgggcct catggcttgg tttggaatcc tcccgacctg
     2101 gcggattctg tccctgccgc tgttcgccgc gctggccgcc acgattacgc tggccgccgg
     2161 gctctggatc tccgcgctgt acgtgaagta ccgggatgtc gggcatattc taccgatgtt
     2221 catccagctg tggatgttcg tgtcgcccat cctctacccc gtgagcaagg tgccggagtc
     2281 ctggcgagcg ctctatgcgc tgaatccggt cgtgagtgtg gtcgagggat ttcgctgggc
     2341 gcttattccc gggtttcagg tcnnnnnnnn nntgttctta ccaagtctgg gtgtggtgtt
     2401 cgtcgtcttt gtcggcggac tcatctattt ccgttcgatg gaacgaacgt ttgcggacgt
     2461 gatttgaatg agcgatattg ccatcaaagc agactgcctt tcgaagcact atcggctcgg
     2521 agccgcaagc cgtcagcata atacccttcg tgatcatctg atgcatcgcc tccgaggcct
     2581 gacgcgatgg ggtggtggtc cgccggagtc gaacccttcg ttctgggcgc tgaaggatgt
     2641 ctcgtttgag gttaagcgag gtgaagtgct gggaatcgtc gggcacaacg gcgcggggaa
     2701 gagcaccttg ctcaagatcc tttccaggat cactcagccg acgacgggaa cggcggatat
     2761 ttacgggcgt gtcagctcgt tgcttgaagt gggtacgggg tttcattccg agttgagcgg
     2821 tcgagagaac atttacctga acgcggcgat gctaggcatg cgacgagggg aagtgcggcg
     2881 aaagttcgat gaaattgtgg cgttctcggg tgtggaggcg ttcatcgata caccggtcaa
     2941 gcggtattcg agcgggatgt acgtgcggct ggcgttcgca gtcgccgcac acctcgagcc
     3001 tgagattctg atcgtggacg aggtgctggc cgtcggcgac gccagtttcc agcagaagtg
     3061 cctgggaaaa atggaagaag tgagccgaag cgggcggacg gtgctgatcg tcagccacaa
     3121 catgaccgtg atcgaagggt tgtgtgaacg cgccattctg ctcgagaagg ggcgtgtcgc
     3181 aaagatcggc aacacgcacg aagtggtcga gggttatgcc gatgccattc ggggtttggc
     3241 ggggacggcc atcgaagcgc gtcatgaccg ggaaggactc ggcgagatcc tgctcaccag
     3301 gatcgaagtg atggatactc agaggcatcc ggtcgcagcg gcgatcaccg gtcgggatgt
     3361 ggtaattcgg atgtactacc gttgtgccgc cggaaaagaa tttaagaatt gccgcgtcag
     3421 catttccgtg aatggacgga aggcacaaga tctgttcgtc ttatccaccg atatcgtcga
     3481 tccgaaaccc ctgaccctgc gcggtgaagg gtatgtcgat tttattttgc ctgaattgcc
     3541 gctcaccggt ggcgcgtatt tcttccaatc ctatatcgag tcgaacggcc atgcccagga
     3601 ctggatcaag aacgtagcgc ccttttccgt gctggatgcc gacttctatg gcacgggcaa
     3661 gctgtgcccg ccgggatggg aggccaacgg cgtgcttgtt cgctatcgct gggaggccgg
     3721 agagactggt tcgaccggcg cattgccgat ccgtgcccgc gcgagttgat cgtctcacgc
     3781 tcccggtgta ttcatccaat tcggttgatc cccctccgct caccggctct gtcgataaat
     3841 cagtaatcac tccgcaatgg accgagagtg cgttacgatt tgccatctac tgccggatgg
     3901 cgatcgactg aggcgcacgc atgggttccg ggtttgagga tggacagcag gctcagttag
     3961 agctggctgg attgaggcgg tcgctgaaat ggacgaatat ccaacgggaa caattgttgg
     4021 atcgtctcga tctgctgcgc ctcgacaatc agcgtctcca ggaacgtgtg gatgagttgg
     4081 agcgtcagtt agccgagttc aagcaacaac aggctctctt ctagcccgga ctattcatga
     4141 atacctgctc cggcgtccga tcctctcgcc cggcgaggct gttctcgttc ggcctcctcg
     4201 gcggactgcg agtacgcctg cgtcggcctt acgtgcgaga agcctcggcg ggcttccgtc
     4261 tcgaacgcct cgcaacggca ttcatgaata atccgggcta gcgaccgctc gtcgtcatct
     4321 ccctccttgt gttctcttct tcacgcgctc atcgacggtc ccggcatgat aaggtggaaa
     4381 gagtgcggcg gtcggataac gccaccttgg ggaaaggccg tgggatattc atgacccatt
     4441 cgatcaagac ggtggatgat ctgcgttggt ttctgtcgcg cacggcgggg tttcgcggcg
     4501 gacacattac cgatgtgcat ttgtccaagc gccggatctt cgacgaagcc tcgggtcgtg
     4561 aggtgatggc tgggagtatg gcgacggtgg tcgtgcggta tcacgcgcag ggcattctac
     4621 gggtcgccaa acttaccatg ctgggcgtta ccgatctttc cctctttgag caggacggca
     4681 gcgactgctc ttctctgggg gcgattcagg tggagatgag cgagggaaaa ttacgcttct
     4741 gg
//
LOCUS       DAORQN010000114         4707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_710_length_4707_cov_6.733878, whole genome shotgun sequence.
ACCESSION   DAORQN010000114 DAORQN010000000
VERSION     DAORQN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_4707_cov_6.733878"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..424
                     /locus_tag="PK614_06820"
     CDS             152..424
                     /locus_tag="PK614_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB81961.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVESAAANIVDMIGSLDATYPGLKNR
                     LCDEKGDLRRFVNIYVNEEDIRFLNGKETSLKDGDEVSIVPAIAGG"
     gene            427..666
                     /locus_tag="PK614_06825"
     CDS             427..666
                     /locus_tag="PK614_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRB81962.1"
                     /translation="MTSLRFHIRFPENKIKEPIIYQIGHEFKVVTNVRRADVRETTGW
                     MDLELTGETEEIERAIDGIRTKGCVVDPIELNVVE"
     gene            751..1554
                     /gene="moeB"
                     /locus_tag="PK614_06830"
     CDS             751..1554
                     /gene="moeB"
                     /locus_tag="PK614_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRB81963.1"
                     /translation="MEFTEQQIQRYSRHIILNEVGGKGQVKLSKAKVLLIGAGGLGSP
                     AALYLAAAGIGTIGLVDGDVVDLSNLQRQILHTTATIGVPKVESGRRMLSAINPDITI
                     KTYQLNVDTENILGLVSDYDIVLDGSDNFTTRFLVNDACFFARKTLISASMFRFEGQL
                     TTIKPHAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLVIYDALEMKFRKVSRPKDPRCPLCSATPTIKDLGGDYSVACTI"
     gene            1556..2068
                     /locus_tag="PK614_06835"
     CDS             1556..2068
                     /locus_tag="PK614_06835"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRB81964.1"
                     /translation="MPDLSIPQHILDQIVAHARELAPFECCGLLAGTNKTVTHLYRIT
                     NIVAVEGAEKLSTFDDDKVAHLERLSPEERAEIAFVMDMGDFSAAKKDIRKNGLELQV
                     VYHSHPKDPARPSHTDIKIATDYEEIWERINLPVPAYLLVSLMHSPAADIRTYWIADG
                     KVRPADVHIM"
     assembly_gap    2069..2088
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2175..2504
                     /locus_tag="PK614_06840"
     CDS             2175..2504
                     /locus_tag="PK614_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81965.1"
                     /translation="MRFGLLIIALILCLTGVTNPEHTSAVEKSYYSPIINVDVDNSRI
                     LISTLGAVFWVEVPEEAKAHIEKLPQSGLVDIVVETREGQPPLLKTWKVKSGESTCLH
                     FDGKVCK"
     gene            complement(2527..2814)
                     /locus_tag="PK614_06845"
     CDS             complement(2527..2814)
                     /locus_tag="PK614_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRB81966.1"
                     /translation="MNDYVEGVLFEISARDEASLDEKEGVSSGSYQKAELPVLHEGLA
                     EVALVYADPVTIKGNPKQEYIQRINSGLADAKLSDAYATRYVRRYISARPG"
     gene            complement(2977..3288)
                     /locus_tag="PK614_06850"
     CDS             complement(2977..3288)
                     /locus_tag="PK614_06850"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRB81967.1"
                     /translation="MKSVTLGKSISGAEVTNISPHGFWLFIADEELFLSFKEFPWFKD
                     ASVAALVNVQWPHPHHLYWPELDVDLAVESIRHPKKFPLVSKASRPARRPIRRAKIAR
                     S"
     gene            complement(3248..3421)
                     /locus_tag="PK614_06855"
     CDS             complement(3248..3421)
                     /locus_tag="PK614_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRB81968.1"
                     /translation="MHVHVYCERGEAKFWLDPKVELAQNFGLVARQIRVAKNLIEEHD
                     DEIRNSWKKHFGS"
     gene            complement(3660..3869)
                     /locus_tag="PK614_06860"
     CDS             complement(3660..3869)
                     /locus_tag="PK614_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81969.1"
                     /translation="MAGSTGRKTVSVSRGLMMLCDVCYEQVFAENLVDQRNFVSDHES
                     LFDTICMGCTDINRPLVDDMLGSSE"
     gene            complement(3916..4110)
                     /locus_tag="PK614_06865"
     CDS             complement(3916..4110)
                     /locus_tag="PK614_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81970.1"
                     /translation="MVLIYESDPLDNEDARGHFYHVCRGRIDRTEIRLPEGESIYECA
                     VCGVDLEPEDFWIARQKGSV"
     gene            complement(4340..>4707)
                     /locus_tag="PK614_06870"
     CDS             complement(4340..>4707)
                     /locus_tag="PK614_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB81971.1"
                     /translation="FITRLPDGYDSWIGERGVKLSVGQKQRVSIARVLLKDPPIVIFD
                     EATSNIDTETEVKIREALNDLTKGRTTFIIAHRLATLHDVDRIIVVERGTIVEEGDHE
                     ALMKRGGVYAGLYEAQFKI"
BASE COUNT         1095 a         1384 c         1199 g         1009 t
ORIGIN      
        1 cgcttgttgt cgcacacggg tgagtttctc ctgcatcgtt cccgttgatg gaagcggttg
       61 tggatgccgt cggtcgtccg ttccgcatcc agcccagcct ggtgaacttc caaaaaacat
      121 tcaaaatggg aaagaacagt ggtggtgact catgattaag gttcgtattc caacgcccct
      181 gcgtcccctc acaaagggtc agggcgaagt tgaatccgcg gcagccaaca ttgtggacat
      241 gatcggatcg ctcgacgcga cctatccggg gctgaagaac cggctctgtg acgaaaaggg
      301 cgacttgcgc cggttcgtca acatctacgt caacgaagag gacatccgtt tcctgaacgg
      361 gaaagaaacc tctttgaagg acggcgatga agtgtcgatc gttcccgcga ttgccggagg
      421 atagccatga ccagcctacg ttttcatatt cgttttcccg agaacaagat caaggagccg
      481 atcatttatc agatcggcca tgagttcaag gtcgtgacca acgtgcgtcg ggccgatgtg
      541 cgcgagacga ccgggtggat ggatctcgaa ctgaccggcg agaccgaaga aatcgaacgc
      601 gccatcgacg gcatccgcac caaaggctgc gtcgtcgatc ccattgaatt gaatgtggtg
      661 gaataacaat ggtgacgcgc acctcgtgaa gcgtatctcg ttttagggtc agaccgcttc
      721 acgtttcacg aacgacgaga gacgagtcga atggaattta ccgaacagca aatacagcgc
      781 tatagccggc acatcatcct caacgaggtc ggcggcaagg gacaggtcaa actgtccaag
      841 gccaaggtcc tcctgatcgg agccggggga ctcggctcgc ccgcagccct gtatctggcg
      901 gctgccggca tcggcaccat cggcctggtg gacggcgacg tggtggatct ctcgaacctc
      961 cagcggcaga ttctccatac gactgccacc atcggcgtgc ccaaggtgga atccgggaga
     1021 aggatgctgt cggccatcaa ccccgacatc acgatcaaga cctatcagct aaacgtcgat
     1081 accgagaata ttcttggact cgtgtccgac tatgacatcg tgctggacgg ctccgacaat
     1141 ttcaccacgc gcttcctcgt gaacgatgcc tgtttcttcg ccaggaagac actcatctcg
     1201 gccagcatgt ttcgcttcga gggacagctc acaaccatca agccgcatgc cggctaccct
     1261 tgctaccggt gtctctaccc ggaaccaccg ccggccggat tggtcccgaa ctgtcaggaa
     1321 gccggtgtat tgggggtcct ggccggaacc atgggcatcc tgcaagcctc tgaagccatc
     1381 aaggaaattc tgggcatcgg cgagacaatc gcggacaagc tcgtgatcta cgatgcgctg
     1441 gagatgaagt tccggaaagt gtcgcgcccg aaagacccgc gttgccctct ctgcagcgcc
     1501 acgccgacga tcaaagactt gggcggggac tacagcgtcg cctgcaccat ctgatatgcc
     1561 cgatctatcg attccgcaac acatcctgga tcagatcgtc gcgcatgcgc gcgagttggc
     1621 gccgttcgaa tgctgcggcc tgctcgccgg cacgaacaag accgtcacac acctctaccg
     1681 catcaccaac atcgtggccg tcgaaggggc ggagaaactc tcgaccttcg atgacgacaa
     1741 ggtcgcccat cttgaacggc tgtccccgga agagcgagct gagatcgcct tcgtgatgga
     1801 catgggggat ttttcggccg ctaagaaaga catacgcaag aacggcttgg agcttcaagt
     1861 ggtctaccat tcccatccga aagacccggc gcgcccctcg cacaccgaca tcaagatcgc
     1921 caccgattac gaagaaatct gggaacgcat caatctcccc gtccccgcct atctcctcgt
     1981 gtcactcatg cactcccccg cagcggatat tcgcacctac tggatcgccg acggcaaggt
     2041 tcgccctgcc gacgtgcata tcatgtagnn nnnnnnnnnn nnnnnnnnca acaaaaccac
     2101 ctcgctccct ctaccaatcg accggcaacg atgccataat tacgctttga ccttgtcatc
     2161 gaagggagca ttcgatgcga tttggcctgc tcattatcgc actcattttg tgcctgaccg
     2221 gggtgacgaa ccctgaacac acctccgccg ttgaaaagag ctactacagc ccgatcatca
     2281 acgtagacgt cgataacagc cgcattctga tctcaacgct cggcgcagtg ttttgggtgg
     2341 aggtcccaga ggaggccaaa gctcacatcg agaagctacc gcaatcaggg ctcgtagata
     2401 ttgtggtgga aacacgcgaa gggcaaccgc cgctgcttaa aacttggaag gtcaagtccg
     2461 gcgaatcgac ctgcctgcac ttcgacggga aggtctgcaa atagagaaga gacgttgcgc
     2521 ttggcctcaa cctggccggg ctgatatgta tctccgcaca taccgtgtgg cgtacgcatc
     2581 agatagcttc gcatctgcca gacctgaatt gatacgctga atatactcct gtttagggtt
     2641 gcctttgatt gtcactggat ccgcgtagac gagcgccact tcggcaaggc cctcatgcaa
     2701 caccggaagc tctgccttct gatatgagcc tgagctcacg ccttccttct catccaacga
     2761 cgcttcatcc cttgcagaaa tctcgaatag gacaccttcg acgtagtcat tcatcgattt
     2821 gacaacgttc gcatatccac gcgccgaaat aattcaacgg taatcaagaa gacgggcaac
     2881 accaactttc ttgcttggcg agcatcgttc ttccatttgg gcactccaca tattggagcc
     2941 gtatgcaaag tagagtcgca tgattagtgc cccacttcag ctacgcgcta tctttgctcg
     3001 acggattggg cgtcgggctg gtcgcgatgc tttcgagaca agagggaatt tcttggggtg
     3061 cctgatagac tcgacggcta gatccacatc aagctcaggc caatacaggt gatgagggtg
     3121 cggccattga acgttgacaa gcgcagctac ggaggcgtcc ttgaaccagg ggaactcttt
     3181 aaacgagaga aagagctctt cgtcagcgat gaaaagccag aatccatgcg gggagatatt
     3241 tgtgacctca gctcccgaaa tgctttttcc aagagttacg gatttcatca tcatgctcct
     3301 cgattagatt cttagcgacc cgtatctgtc tggcgaccag accaaaattt tgcgccagtt
     3361 cgaccttggg atcgagccag aatttggctt caccgcgttc acaatacaca tgcacgtgca
     3421 tacgtgtttc ctcgcgagaa aagaagtaga accgaaaccc tccagccctg aaaattgtgg
     3481 gactcagatg cgctctcctt gcctgcgagt caactcccga taaaataagc agcacgcctc
     3541 atcactacct gactgagagt gaagtagctc agtcagcaca gcttgtgccg aagcgcgagg
     3601 ttcgaacagc ctctatcgag aggctacgac tcatttcccg taccatagat gccgcaagct
     3661 tactccgaac tccccaacat gtcgtcgact agcggacggt tgatatccgt gcaccccatg
     3721 caaatggtat cgaacaacga ttcgtggtcg gacacgaagt tccgctgatc gaccagattc
     3781 tcggcaaaga cctgttcgta gcacacgtca cacagcatca tgaggccgcg tgacacggac
     3841 actgttttcc ggcctgtact ccccgccata acagacttcc tttcatctat attcacccgg
     3901 tgcgcgcagc ctgcgctaca ccgatccctt ttgcctcgcg atccagaaat cttcaggctc
     3961 tagatcaaca ccacacaccg cgcactcata aatcgactct ccctccggca accggatttc
     4021 cgtccggtcg atccggcccc gacagacatg ataaaaatgc ccccgggcgt cctcattgtc
     4081 gagcggatca ctctcgtaaa tcagcaccat atcccttact ccctttgatc atcattcatg
     4141 aagtctgcgc atccgtaaca ctcaatattc agtcgactct tggatattga ccgcgtattt
     4201 cttatacaca ttcgtaagga tgttcaaaat ggccgtccag caagtccgca gcgacgtgaa
     4261 tgagcgaggc gtacccttgc ggtacgttga gctctttcac gatgcgagaa cgatgttggc
     4321 ggactttttc agcatcctgt taaattttaa actgggcctc atacagcccc gcatacaccc
     4381 cgccccgttt catgagggcc tcgtgatcgc cttcctcgac aatcgtcccg cgctccacca
     4441 cgatgatgcg atccacgtca tggagcgtgg ccaggcggtg agcgatgatg aaggtggttc
     4501 gccccttggt cagatcgttc agggcttcac ggattttcac ttcggtttca gtgtcgatat
     4561 tggaggtggc ctcgtcgaag atcacgatgg gcggatcttt cagcagcacg cgggcaatcg
     4621 agacccgctg cttctggcca accgacaatt tcacaccacg ctccccgatc cagctatcat
     4681 acccgtccgg caagcgggtg ataaagt
//
LOCUS       DAORQN010000115         4704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_711_length_4704_cov_6.558615, whole genome shotgun sequence.
ACCESSION   DAORQN010000115 DAORQN010000000
VERSION     DAORQN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_711_length_4704_cov_6.558615"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1799)
                     /locus_tag="PK614_06875"
     CDS             complement(96..1799)
                     /locus_tag="PK614_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRB81972.1"
                     /translation="MPTRPTASLLLDNPTISKTLDDLASSHTIERIWTRDHTLWKPSP
                     TEIDNRLGWLTVLDHMQDGLAELRSFEQAAREARITDVVLLGMGGSSLGPEVLRCTFG
                     SAKGSPKLWVLDSTVPGWVRQVTKAIQPARTLFLVASKSGGTIEVMSLFAHFWELVRR
                     TKGNRGGAQFVAITDPGTGLEQLARERGFWRTFTNQPDIGGRYSVLSYFGLVPAALLG
                     LDVSKLLSRALAMREACRHTIHPKDNPGAALGGMMAAMARAGRDKVTLLTSPALNSFG
                     LWVEQLLAESTGKEGTGIVPVAGEPLASPTAYGADRLFVTLRLKGDRNAALDRSIITL
                     QRAGHPVFRLNLADRYDLGGEFFRWEFATAVAGHALGIHPFDQPNVQESKDNTGRVLK
                     EVETQGRFPSLPMLTPRQALTQLLEQATPNRYLAILAYTTPSPQLDASIRALRKAVML
                     RHHIATTAGYGPRYLHSTGQLHKGGPNSGLFLELVDSMKPDLPVPEKFYSFGTLAQAQ
                     AVGDLQSLQTHQRPAVRIALGPNPVRTIRSLIAMLTPARAGRRKPAAKKRPLPKRRTH
                     R"
     gene            complement(1975..3123)
                     /locus_tag="PK614_06880"
     CDS             complement(1975..3123)
                     /locus_tag="PK614_06880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRB81973.1"
                     /translation="MRRLTHIFRTFCSLALAGFLAMSLGGCVETVPDELVEAIEGIDR
                     DLVTLRASDISPEAYSKFSREWISLRARIQSEENIVRWPWEENELETDLESLQVEGSQ
                     LVADVNTRLQAQRTAAETKLAKVEQRLRLLNTRVGDIGSRVVLGEHPVETELLVRQAR
                     LFIEQGQFDHAIQASDRAGKILLTQTALLTNELGRYADDDLIAAWRESAQRTIEWSRT
                     HRAQAIVISKADRVLSLYKNGKKVLTYPIRLGSRGIRAKQHYGDGATPEGEYRIHRKR
                     GSGQTPYFRALILDYPNADDRRRFEEAQQSGLIPKSQHMPGLIQLHGIAQGITDQPYG
                     SILLDNPQIAVLFDQVSVGTPVTIVGALESQNSISLVLADLGDQEEET"
     gene            complement(3529..4395)
                     /locus_tag="PK614_06885"
     CDS             complement(3529..4395)
                     /locus_tag="PK614_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81974.1"
                     /translation="MKGRIACSADRLSLLEAAMISSVVEPNAFQIGKQYQAERRVQMT
                     DASDTEVTSSVMGNSGLYEQTIRLIDGHLEAKCSCTLSEQPICRHGVAALLEYQRWSK
                     ARVVPKPRVAAPRPEAEKPAAPVVSSGDMKLSELNQFTEWMQRTVQAIQAGKPLPDQP
                     SIGPGLVLTWTQVIQQMDERRRESEVAQVELDADIRNREAVVAHLTQDLEASVNESKS
                     LQVICRDLQREIEAQKAAVSKTTDLARQVEQVEVEVKAMAALLTDKGRRLEGLSDTCR
                     DVSSMLKSLGKA"
BASE COUNT          891 a         1308 c         1446 g         1059 t
ORIGIN      
        1 tcgcctgctt gcccgcttcc aggaagagac ctgcagcggc acgggtcagt tcctgggcat
       61 cggtgaaggt gtggattgct ggagcctggg acatgtcaac ggtgagtgcg gcgtttgggc
      121 agaggacgtt tttttgccgc gggtttacga cgaccggctc tggccggtgt gagcatggca
      181 atgaggcttc ggatggtgcg cacgggattg ggtccgaggg cgatgcggac ggccggccgt
      241 tgatgggtct gcaacgattg aaggtcgccg acggcttgcg cctgtgccag cgtgccgaag
      301 gaatagaact tttcggggac cggaaggtca ggtttcatgc tgtccacgag ttcgaggaac
      361 aggccgctgt tcgggccacc tttgtggagt tggcctgtgg aatgcaggta ccggggacca
      421 tagcccgccg tggtggcgat gtggtggcgc agcatcaccg ccttgcgcaa cgcacgtatt
      481 gaggcgtcca gctgagggct gggagtggtg tacgccagaa tggccaggta gcgattgggg
      541 gtcgcctgtt cgagcaattg agtcagtgcc tgcctgggcg tgagcatcgg cagtgagggg
      601 aatcgtccct gtgtttcaac ttcttttagc acgcgtcctg tgttgtcttt gctctcctgc
      661 acgttcggct gatcgaacgg atggatgccg agcgcgtgtc ctgcgaccgc ggtggcgaac
      721 tcccaacgga agaattcccc gccgaggtcg tagcgatccg ccaggttcaa tcgaaatacc
      781 ggatgcccgg cccgttgcaa cgtgatgatg gatcgatcca gcgcggcatt gcggtcgcct
      841 ttgagtcgaa gcgtgacgaa gagtcgatcg gctccatagg cggtcgggga ggccaggggc
      901 tcacctgcca cgggaacgat cccggttcct tccttcccgg tgctttccgc gagaagttgt
      961 tccacccaga gtccaaatga attgagcgcg ggggaggtca gcagagtgac cttgtcccgc
     1021 cctgctcggg ccatcgcagc catcattcct cccagggctg ctcccgggtt gtcttttggg
     1081 tgaatggtgt gtcggcaggc ctcacgcatg gcgagcgccc ggctgaggag tttgctcacg
     1141 tcgagtccca gcagtgcggc gggcaccagg ccaaagtagg agaggacgga gtatcgtccg
     1201 ccgatgtccg gttgattggt gaacgtgcgc caaaacccgc gctcgcgcgc cagctgttcg
     1261 agtccggtgc cgggatccgt gatggcgaca aactgggctc cgccgcgatt gcctttggtg
     1321 cgccggacaa gttcccagaa gtgagcgaag agtgacatca cttcaatcgt tccgccggac
     1381 ttgctcgcca cgagaaagag ggttcgcgcc ggttgaatcg ctttcgtcac ctgacggacc
     1441 cagcccggga ctgtggagtc cagtacccag agtttggggg accctttggc cgaaccaaat
     1501 gtgcagcgga ggacttcggg tcccaagctg ctgccaccca ttccgagaag cacgacgtcg
     1561 gtgatgcgag cttcccgggc agcctgctcg aagctccgta actcggcgag cccgtcctgc
     1621 atatggtcga gcaccgtcag ccagcccagc cgattatcga tttcagtcgg actgggcttc
     1681 cagagagtat ggtctcgtgt ccagatgcgc tcgatcgtat ggctgctcgc caggtcgtcc
     1741 agcgttttgc tgatggttgg gttgtcgagc aggagcgatg cagtgggacg ggtcggcata
     1801 acacgctctc ttccgttagt ggaggaacga atcgatggac ctcggctctt tcgtatctta
     1861 ggttcgagcg tgtaaaaagg caaacaaaga gaagccggag actgcgcagg agtgtcttga
     1921 atgagtgggt gtgggtgctg tgcggttgga gcagtcgcgc ggaaggatgg ggatctaggt
     1981 ctcttcttcc tgatcaccta agtcggccag tacgagagaa atggaattct gcgattccag
     2041 tgcgccgaca atagtgacag gtgttccaac agaaacttgg tcaaagagca cggcaatctg
     2101 aggattatcc agcaggatgc tgccgtacgg ttgatccgtg ataccttgtg cgatgccatg
     2161 gagctggatc aggccgggca tatgctggct cttgggaatc aaccctgatt gttgagcttc
     2221 ttcaaagcgg cgtcgatcgt cggcattggg atagtcgaga atcagggcgc ggaaatacgg
     2281 ggtctgtccc gacccgcgtt tgcggtgaat gcgatattcg ccttcgggcg ttgcgccgtc
     2341 gccgtagtgc tgtttcgccc ggatgcctct cgaccccagg cggatgggat aggtcaatac
     2401 tttcttgccg ttcttgtaca gagacagcac tcggtcggcc ttgctgatga caatggcttg
     2461 cgcacgatgg gtgcgcgacc actcaatggt tcgttgggct gattctcgcc aggctgcaat
     2521 gaggtcgtca tcggcatacc ggcccaactc gtttgtcagc aaggcggttt gggtgagcag
     2581 tattttcccc gcacgatctg aagcctggat ggcatggtcg aactgtccct gttcaatgaa
     2641 caggcgagct tgtcgtacaa gcagttcggt ttcaaccgga tgttcgccga gtaccacacg
     2701 gctgccgata tctcctacgc gggtgttcaa gaggcggagc cgttgctcga ccttggcgag
     2761 ttttgtttcg gcggcggtgc gttgggcttg gaggcgcgtg ttcacgtcgg ccacgagctg
     2821 cgacccttcg acttgcaggc tttccaggtc ggtctccagc tcattttctt cccacggcca
     2881 acggacgata ttttcctcgg attgaatgcg ggctcgtaag gagatccatt cgcgggagaa
     2941 ttttgaatag gcttcggggc tgatgtcgga tgcccgcagc gtgacgaggt cgcggtcgat
     3001 gccctcaatg gcttcgacca gctcgtcggg gaccgtttcg acgcacccgc ccaggctcat
     3061 tgccaggaat ccggcaaggg ctagactgca gaaggtgcgg aaaatgtggg tcaggcgtct
     3121 catcgtttca ttctatctcg cagggagcgt ttctcgcgag ctcttctcgt ttttgtcact
     3181 gacacgtgcg gacaagcatg gccatttcag aatgcgtaca tccggtttct gctggggcat
     3241 ccgcaggccg ctaggaacac ctccaatccg ttgcggccca cgaccccggg ctctaattaa
     3301 cgcaagctgg atgccagggt ggagattgtt gcggactgga acgattatct ttccagaaca
     3361 attagttaga tcgattcggc aagacgcgcc ctctgcctgt ttctgtggca tgtttgtcac
     3421 agtcgggcaa gagtgcctca agagttcgag gttgagtagc gatcgcgcgg tcagaaacct
     3481 ttccccattc cagctctggt tctccttccg tgaccaccga cttgccaatc aggcctttcc
     3541 caaagacttg agcatcgaag acacatctcg gcaggtatcc gaaagacctt ccaagcgacg
     3601 ccccttgtcc gtcaaaagag cggccatcgc tttcacctcg acttcaacct gctcaacctg
     3661 ccgggcaaga tcggtcgtct tgcttaccgc agccttctgc gcctcaatct cccgttgaag
     3721 atctcggcaa atgacctgta gtgacttaga ttcattgacg gaagcctcca gatcctgggt
     3781 gagatgggcc acgaccgctt ctcgattgcg aatgtccgca tcaagctcaa cctgagcgac
     3841 ttcgctctct cgcctccgtt catccatctg ttggatcacc tgggtccagg tcaacacaag
     3901 gcccggtcca atagaaggtt ggtctggaag gggcttcccg gcttgaatgg cctgaaccgt
     3961 tcgctgcatc cactccgtaa actggttcaa ttcgctcaac ttcatatcac cggaggacac
     4021 gacaggggcg gccggttttt ctgcttcggg gcggggagcg gcgactctgg gcttgggtac
     4081 aactcgtgcc tttgaccacc gctgatactc aagcagcgca gccacaccgt gtcggcagat
     4141 cggttgctcg gataacgtgc aagaacattt ggcttccaga tgaccatcaa tgagacgaat
     4201 cgtctgctcg tacaacccgg aattgcccat gaccgacgac gtcacttccg tatccgacgc
     4261 atccgtcatt tgcacgcggc gttctgcctg atactgcttt ccgatttgga acgcgttggg
     4321 ctccaccacc gagctaatca tggccgcctc caacaaactc agacgatcgg ccgaacacgc
     4381 gattctgccc ttcatagtgt cacctgtgga atgattagta ccgctgagtc gtccttgcag
     4441 cctaccgagt gatcgccgca gtgtcatgga atcaccacct ttgacgtcca cctatccagc
     4501 gagacttgag tgtatgacag gttctcctga acgaccatcc cgcaggcggg gggaattgtg
     4561 agaaatggat cgaggccgcg cagaattttc gtgccgccac tggagattct tatcggcagc
     4621 gtcgaggagg tccttgagcc ccctgggggg tgagccacac atagtcagca atcccttctt
     4681 gcatggcccg atgaagctct gcag
//
LOCUS       DAORQN010000116         4689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_714_length_4689_cov_5.810315, whole genome shotgun sequence.
ACCESSION   DAORQN010000116 DAORQN010000000
VERSION     DAORQN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_714_length_4689_cov_5.810315"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..152)
                     /locus_tag="PK614_06890"
     tRNA            complement(66..152)
                     /locus_tag="PK614_06890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Leu,seq:caa)
     gene            331..612
                     /locus_tag="PK614_06895"
     CDS             331..612
                     /locus_tag="PK614_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HRB81975.1"
                     /translation="MADVQCVTCGQAGEAITDMLFLGKLEAEIKAKVCKPCWKKWEGM
                     RVMVINEYQVNLGDESGRELVKKQMKAFLKLGEQTDTSKLDQNFRPAGS"
     gene            855..1310
                     /locus_tag="PK614_06900"
     CDS             855..1310
                     /locus_tag="PK614_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRB81976.1"
                     /translation="MITQMRVKGLIFDPYNNAYIVVLRDEENSDMLPIWVGKSEASAI
                     GLALESVTAPRPMTHDLMKSFLETFEAKIISVVITDLNENTYFATIHLMYEDSEYTVD
                     SRPSDAIALALRTSAPIFASEKVIKKQSSEELEQWLENLKPEDFGKLDT"
     gene            1313..1801
                     /locus_tag="PK614_06905"
     CDS             1313..1801
                     /locus_tag="PK614_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRB81977.1"
                     /translation="MDSANQQNEPSELVALTVKQVLDDGNTDTRIVVLKNDDASVTLP
                     IWVGSAEGNAIRLAMERVVTPRPMSHDLIRSFADHLGVRIERVVITDVKGSTYYASVA
                     FASKGVHRTLDARPSDAIALALRADCPIYATQDVLNRRTAVHLDAWINKLDTKNIEPQ
                     QA"
     gene            1852..2283
                     /gene="rplM"
                     /locus_tag="PK614_06910"
     CDS             1852..2283
                     /gene="rplM"
                     /locus_tag="PK614_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRB81978.1"
                     /translation="MTKTYLEKPADVKRKWFLVDADGKTLGRLAAKVATLLRGKHKPT
                     FTPHVDTGDHVVIVNAEKIRLTGNKMEDKTYSYHTGYPGGLKTLTAEHIHKKDPTKLL
                     TRAIEGMLPKNPLGNQMAKKLRVYAGSTHPHHAQQLESLSL"
     gene            2326..2718
                     /gene="rpsI"
                     /locus_tag="PK614_06915"
     CDS             2326..2718
                     /gene="rpsI"
                     /locus_tag="PK614_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRB81979.1"
                     /translation="MAAITQYATGKRKSAIARAWVTAAAGDIVVNEKPLEKAFPRMTL
                     RNVIQFPFELAGVAGKYSVRATVYGGGPAGQAGALRHAISKALVVMSAAFRSPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            2831..3904
                     /gene="argC"
                     /locus_tag="PK614_06920"
     CDS             2831..3904
                     /gene="argC"
                     /locus_tag="PK614_06920"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRB81980.1"
                     /translation="MTSVRVAVAGGSGYTGAELLRLLSQHPNVQLTAVTSEKSAGTPV
                     STVYPHLQGIVPLTFEALAPEALAERADVLFLALPHTKSMGPVASCMKAGKRVIDLSA
                     DFRIKDPRTYETWYQTAHACPHLIENAVYGLPELHRSAIAQARLVASPGCYPTAAILQ
                     LAPLIAHGLIVPDTIVIDAKSGVSGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEIE
                     QELSGLAGTRGQAVTGETAGVTIAFTPHLVPMNRGILSTAYARMKGKLDVVELRALYR
                     DFYKGERFVRLLEGAMPNPRHVRGANYCDLAVHTDTRAGWVVTVSAIDNLIKGAAGQA
                     IQAMNLMLGYPEETGLVAPGIYP"
     gene            3960..>4689
                     /locus_tag="PK614_06925"
     CDS             3960..>4689
                     /locus_tag="PK614_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRB81981.1"
                     /translation="MKIITGGVTAPIGFRAAGMYCGIKKKQKKQLDLALLVSEQEGPV
                     AGVFTTNQVVAAPVVLDRLHLKKGIGRAILVNSGNANACTGPDGMRVAKEMAQVVATA
                     VGSAPHTIFIGSTGVIGQPLPIDCIKNAVPSLLARVTRRGGNEAAQAIMTTDLKPKQV
                     AVRARLGGRMVTVGGMAKGSGMIHPNMATMLGYLTTDAVISRSALQTALSQAVDQSFN
                     CITVDGDTSTNDTVLCLANGMAGNQ"
BASE COUNT         1098 a         1330 c         1344 g          917 t
ORIGIN      
        1 caccagacgg caatgtctgc atgagcgcac tggttgccga gaccatggaa ggggtgtcga
       61 aatgatggtg cccagaggga gggtcgaact cccactctct tgcgagaacc ggattttgag
      121 tccggcgcgt ctgccagttc cgccatccgg gcacgtgtgt catgcgacgt caattccttg
      181 gcttgccact cggctttttg aagggacgca tttttaacat gaacccgagt aggcgtcaat
      241 tggcacagca cactcttttt tctgctgcat gcgaatgcta gaatgccgcg cgaccgacaa
      301 ataaagatca ggcagattga ggaggcgact atggcagatg tgcaatgtgt cacctgcgga
      361 caagcaggcg aagcgattac ggatatgctg ttcctcggaa agctcgaagc cgaaatcaag
      421 gccaaggttt gcaagccctg ctggaaaaag tgggaaggga tgcgggtcat ggtgatcaac
      481 gagtatcaag tcaatctcgg cgacgagagc ggccgcgaac tcgtcaaaaa gcaaatgaag
      541 gcgttcctca aactgggaga acaaaccgac acgtccaaac tggatcaaaa ctttcgtcca
      601 gccggctctt aaggttacga cttccaatct gactgtggtg tcaggcagaa ccgcttcagg
      661 cagaaccgtt tatgatcggc gcctgttcat cacacggttg aggtccagtg gaaccttgag
      721 gcctcacgag tggtgaccca tcggcgttct cttcaaccgg tgccagatcg aattcattga
      781 cactctgatt tcggaagtgc tagagtgccc tattcttgag gtttttagcc gacatcggga
      841 aggatactcc cgccgtgatt acacaaatgc gggtaaaggg tctgatcttc gacccctaca
      901 acaacgccta tatagtcgtg ttgcgggacg aagagaattc ggacatgctc ccgatctggg
      961 tcggaaaatc agaagccagt gccatcggcc ttgcgctgga gagtgtcacc gcaccgcgtc
     1021 ccatgaccca cgacttaatg aagtcgttcc tggaaacgtt cgaggcgaag attatcagcg
     1081 tggtgattac agatttgaac gagaacacct atttcgccac gattcacctg atgtacgaag
     1141 actcggaata tacggtcgat tctcgcccca gcgacgccat cgcgctggcg cttcggacca
     1201 gcgcgccgat ttttgccagc gagaaggtta ttaagaaaca gtcgtccgaa gaacttgaac
     1261 agtggcttga aaatttgaag ccggaagatt tcggaaaact ggatacctag ccatggactc
     1321 ggcgaatcag cagaatgagc cctcggagct cgtcgctttg accgtcaagc aggtgctgga
     1381 cgacggcaac accgataccc gcatcgtcgt gctgaaaaat gatgacgcga gtgtgacgct
     1441 accgatctgg gttggatctg cggaaggtaa cgcaattcgt cttgccatgg agcgtgtggt
     1501 aactccccgc cctatgagcc acgacctcat tcggagcttt gcggatcatc ttggcgtgcg
     1561 gattgaacgg gttgtgatta cggatgtgaa aggcagtacc tattacgcca gcgtggcctt
     1621 tgcgtcgaag ggcgtgcatc gtaccctgga tgccagacca agcgacgcga tcgccctcgc
     1681 acttcgcgcg gactgcccga tctatgccac gcaagatgtg ctcaaccggc gcacggccgt
     1741 tcacctggat gcttggatca ataaactcga cactaagaat atcgaacccc aacaggcgtg
     1801 aggcctgata cctcacatta gaatcctttc gtgcaggagt agatggagac catgacaaaa
     1861 acgtatcttg agaagccagc tgatgtgaaa cggaaatggt tcttggtgga tgccgacggc
     1921 aagacgctcg gtcgccttgc ggcgaaggtg gccaccttgc ttcgaggcaa gcataaaccg
     1981 acgtttaccc cccacgtgga taccggcgac catgtcgtca tcgtcaatgc ggagaagatc
     2041 cggttgaccg gcaacaagat ggaagacaag acctattcgt accacacggg atatccaggc
     2101 ggactcaaga ccctcaccgc cgaacatatc cacaagaagg acccgacgaa gctcttgacg
     2161 cgcgccatcg aaggcatgtt gcccaagaat ccccttggga atcagatggc caagaaactg
     2221 cgtgtgtacg caggttcaac ccatccacat cacgcgcagc aactggaatc actttcgctg
     2281 tgatcattat tgttcggatt cgatctgtga aggaggccgc ctgatatggc agcaattacg
     2341 cagtacgcaa ctgggaagag aaagagcgcg atcgctcgtg catgggtgac cgccgctgct
     2401 ggtgatatcg tcgtcaacga gaagccgctg gaaaaagcgt ttcctcgcat gaccttgcgg
     2461 aatgtcattc aatttccgtt tgaactggcc ggcgtggccg gtaagtattc cgtccgggcc
     2521 accgtctatg gtggtgggcc tgcgggtcag gctggagcct tgcggcatgc catctcgaag
     2581 gcgctggtcg tcatgagcgc tgcattccga agcccgctca agaaggaagg tcttctgacc
     2641 cgtgactccc gcgtgaaaga gcgcaaaaag tacggccaga agggcgccag aaagcgcttc
     2701 caatactcga aacgataaag aaacactgga gcgacaaggt gtcaaaaggg aaggctccgc
     2761 agccttccct ttttttatgt gcgcgctgaa ctgatcgagc aacaattcgc gataggccgg
     2821 gaggggtgac atgacgtcgg tacgagtagc agtggcaggg ggcagcggat acaccggagc
     2881 ggagttgcta cggctcctgt cgcaacatcc caacgtgcaa ttgactgcgg tgacctccga
     2941 gaagtcggcc ggaacccccg tctccaccgt gtatccccat ttgcagggca tcgtgccgct
     3001 gaccttcgaa gccctggcgc ctgaggccct ggcagaacgc gccgatgtgc tcttcctcgc
     3061 ccttccccat acaaaatcca tgggcccggt agcgagttgc atgaaagccg gcaagcgcgt
     3121 gatcgacctg agcgcagact tcaggatcaa agatcctcgc acctatgaaa cctggtatca
     3181 gaccgcgcac gcctgtcccc atctgatcga aaacgccgtg tatggcctgc cggaactgca
     3241 tcgatcagcc atcgcacagg cccggctcgt ggcctcccca ggctgttacc cgaccgcagc
     3301 gattctgcaa ctggcgccgt tgatcgccca tggcctcatc gtgccggata ccattgtgat
     3361 tgatgcaaaa tctggcgtgt cgggcgcagg aagaagtcct gccctgccct accacttccc
     3421 cgaagcacac gaatcactgg agccctataa gatcggccag catcgacaca tcccggaaat
     3481 cgaacaggaa ctgagcgggc tggcaggaac tcgtggacag gcagtcacag gagagaccgc
     3541 cggtgtcacc attgcgttca cgccgcacct ggttccgatg aatcgaggta tcttgagcac
     3601 cgcctatgca cgcatgaagg gtaagctcga tgtcgtcgaa ctgcgggcgc tctatcggga
     3661 cttctacaag ggagaacgat tcgtgcggct gctcgaaggc gcgatgccga atccccgaca
     3721 tgtgcgaggc gccaactact gcgatctcgc agtccacacc gatacgcgag ccggctgggt
     3781 cgtgaccgtg tcggccatcg acaacctgat caagggagca gcgggacaag cgattcaggc
     3841 tatgaatctc atgctcggct atccggaaga gacgggcctg gtggcgccgg gtatctatcc
     3901 ataatttgag tcctgggcga ctccgcgcta cggaccattt ctcaccacac aggatcacta
     3961 tgaaaatcat cactggaggc gtcactgcac cgatcggctt tcgggctgcc gggatgtatt
     4021 gcggcatcaa gaaaaagcag aagaaacaac tcgacttggc cctgctggtc tccgagcagg
     4081 agggacccgt cgccggcgtg ttcaccacca atcaggtcgt ggctgctcca gttgtgctcg
     4141 atcgcctgca tctgaagaag ggcatcggcc gggccatcct cgtcaacagc gggaacgcca
     4201 acgcctgcac cggacccgac ggaatgcgag tggccaagga gatggcgcag gtggttgcca
     4261 cggccgtcgg cagtgcgccc catactatct tcatcggatc gaccggcgtg atcggtcaac
     4321 cacttccgat cgactgcatc aagaacgccg tcccgtccct cctcgctcgc gtcacgcgcc
     4381 gcggcggaaa cgaagcagcc caagccatca tgaccacgga cctcaaaccc aaacaggtgg
     4441 cggtgcgcgc ccgcctcgga gggcggatgg tgacggtggg aggaatggcc aagggttccg
     4501 ggatgatcca tcccaacatg gccaccatgc tgggctatct cacaactgat gcggtcatca
     4561 gccggtcggc gcttcagacg gcgctcagtc aggcggtcga tcaatcgttt aactgcatta
     4621 ccgtcgacgg cgatacgagc acgaacgaca ccgtcctttg ccttgcgaac ggaatggcag
     4681 gcaatcaga
//
LOCUS       DAORQN010000117         4687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_715_length_4687_cov_7.146805, whole genome shotgun sequence.
ACCESSION   DAORQN010000117 DAORQN010000000
VERSION     DAORQN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_4687_cov_7.146805"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..613)
                     /locus_tag="PK614_06930"
     CDS             complement(278..613)
                     /locus_tag="PK614_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81982.1"
                     /translation="MTLLLDPQQRSTRPVWHVYFSHTLRYVLAPLLFISSGFFTANFI
                     VSGHYTWPRTSRVFVLTLTLLILAYEFIYKDHTVRSTSPERAFNAVLYSCMIPYALGV
                     LMMLGLAKL"
     gene            complement(610..900)
                     /locus_tag="PK614_06935"
     CDS             complement(610..900)
                     /locus_tag="PK614_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81983.1"
                     /translation="MSIRKAAGEWEPMLLGIEPRNCKVCQQGLYRDKTMLRGGWSRCA
                     VCDEFVHYSCLASGKISFLKQRPRICKTCRTAEEQAKQAAPPPSSQPAPVGS"
     gene            complement(1093..1326)
                     /locus_tag="PK614_06940"
     CDS             complement(1093..1326)
                     /locus_tag="PK614_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRB81984.1"
                     /translation="MTTLALTIEKQARKLSPTERERLAERLMAGMNGERLTSVDEAWV
                     AEAEQRFSAWKRKLTKPLSVAKSLRDIRKRLHL"
     gene            complement(1443..4121)
                     /locus_tag="PK614_06945"
     CDS             complement(1443..4121)
                     /locus_tag="PK614_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB81985.1"
                     /translation="MITLKDYQERVLESLGDFFRLTAQTKKPEVAFREVTRRFGEAAP
                     YFPVSAAGLGPDMPYVCLRVPTGGGKTLLACYAAGLAQRQFMRAERAVVLWLVPSHTI
                     LDQTADALRDPRHPYRRALELACGAVEVMTIDEALRLSRAVIDGQTVVIVSTIQSFRV
                     EDTTGRRVYDQNGAFSEHVMNVPPDRFADLFSGADGKPKPSLVNALRLRRPIVIVDEA
                     HNARTDLSFATLGNVLPSCIVEFTATPAREKTPSNVLHRVSAAELKTAQMVKLPLRVV
                     TRHPSQRDHLLAEAITLRTDLERLAVLEGQQTAEYIRPILLIQAERVDACEPLRERLV
                     REFGLSNDEVKISVGRLDELKGVKDIVSPKCLVRVIITVEKLREGWDCPFAYVLCSLK
                     ETWSATAIEQIVGRILRLPNAKAKQHPDLNCAYAFSVSDSITAVLAELREALEHNGFT
                     KAEAERIILSVPQGTLPLGVQPQTVTVGPDEIDPTVVQVQEPALGGKVRIDAASGAIT
                     IVVPLDRDDLEKVQSCVTTPGAKARLAEAAEMVRQAEQAFGGTGKPRKPSPYEQQLDF
                     LVPLLCFAENGMLYEFESTFLLDHPWKLSEKDASLPAAYNPLARPYGKVGVIDVGQKG
                     DVQTTLLGDTGDADFVGTLHQQMFQFSGQDDWSLERLAAWLDREIDHHDIPVGESAEF
                     LRKVIRGLTAKYGIADIGTLALDRFRLRDEIAARIQDHREGERKASFQMLLHSDSPLT
                     VTEERTMNFKTMGYEPSRLYEGGFQFQKHYFGPKPGELTEKTAEGRITEEFQCAQFLD
                     GLPQVRFWVRNLARKSTSFRLQTSKDWFYPDFLCQLMDGRTLAVEYKGKHLFDGVDAE
                     DKRAVGEIWASRSGGRCLFVMPTAGDFSTIRKMLDA"
     gene            complement(4118..>4687)
                     /locus_tag="PK614_06950"
     CDS             complement(4118..>4687)
                     /locus_tag="PK614_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRB81986.1"
                     /translation="ALDSLEPLILVIRGHRIILDADLARLYGVTTKRFNEAFKRNRQR
                     FPDDFAFQLTTAEFSNLRSQIATSSLQHAESTGVHPKWSQIVTTSHGGRRYCPRAFTE
                     HGAVMAANILRSERAVQMSVFVVRAFVRLREQIAANAAILKRLAEIDRTLLGHDTALL
                     DVYEKLLPLLQPPPAAPTRRIGFQSKGKA"
BASE COUNT         1031 a         1422 c         1303 g          931 t
ORIGIN      
        1 cgatgatcag ccgccccgtc tcgtcgattc gcttcaacga caggcacaag ggatggacgg
       61 cgaaacactg ttgcacgaag gcgccgcttt gaaatcgctg ccccatagcc acctaaatag
      121 aaggacccaa agagcctatg gcgtatagct tatagcggaa ggcaagaaat cgagggtggg
      181 agtacttgct atcagctaca ggctatcaac catacgctct tatcccctga gatacgcttc
      241 acgtcttacg tttcacgttt tacgcttcaa cgtgccgcta caacttcgcc aaccccagca
      301 tcatgagcac cccgagggcg taggggatca tacaggaata cagcacggcg ttgaaggctc
      361 gctccggcga agtcgatcga acggtatgat ctttgtaaat gaactcgtag gcgagaatca
      421 gcagcgtgag ggtcagcacg aagacccgac tcgtgcgagg ccaggtgtag tgcccgctca
      481 cgatgaaatt ggccgtaaag aagccgctgg aaataaacag aagcggcgca agcacatagc
      541 gaagggtgtg ggaaaagtag acgtgccaga caggtctggt ggatcgttgc tgcggatcga
      601 gtaagagggt catgacccga ctggtgcggg ttggctggaa ggcggcggag cggcttgctt
      661 cgcctgttcc tcggcggtcc ggcaggtttt gcagatgcgc ggacgctgct tgaggaagct
      721 gatctttccg ctggcaagac aactatagtg cacaaactca tcgcacaccg cgcaacgcga
      781 ccagccgccg cgcaacatcg tcttatcacg gtagagaccc tgctgacaga ccttgcagtt
      841 gcggggctcg atccccagca gcataggttc ccactcaccg gcggctttac gaatactcat
      901 agcgcggaag tatagcgaag gggtccaaga aaaaacaagg agggagagga gggagggttg
      961 gcccggtgat cccccgtgct cgcgcaacgc gcggcctcgg aaggccctcg ttggacgcgc
     1021 gcagttgggg gaccaccagg ccaccctcca ggggaaatag gggcgagggg aaacactaga
     1081 agattactag gtttatagat ggagtctctt gcggatatcg cgcaacgact tggcgactga
     1141 cagcggctta gtaagctttc gcttccaggc ggaaaatcgt tgttctgcct ctgcgaccca
     1201 ggcttcgtcc actgaggtca ggcgttcgcc gttcatccct gccatgagtc gctcagccaa
     1261 ccgttcacgc tcagtcggcg aaagcttccg ggcctgtttt tcaatagtca gggcaagggt
     1321 cgtcatcggc tccactcctt gaaaaatgtc tcagaggccc tacgctgctt tgtgaaactc
     1381 tacaacctgc tggaattctc tgcaacccat gaccctcctg agaccggtat cgtgatcgtg
     1441 ggttatgcgt ccagcatctt acgaatcgtt gaaaaatcgc cggccgtcgg catcacgaag
     1501 agacagcggc caccgctccg ggaggcccat atctctccaa ccgctcgttt atcttccgca
     1561 tcgacaccgt cgaagagatg tttcccttta tactcaacag ctaacgtccg tccgtccatc
     1621 aactggcaga gaaaatccgg atagaaccag tctttggatg tttgcagccg gaaggaagtc
     1681 gatttgcgtg caagatttct cacccagaat ctcacctgcg gcagtccatc gagaaactgc
     1741 gcacactgaa actcttctgt gatccgccct tcggccgtct tttccgtcaa ctcaccgggc
     1801 ttgggtccga agtaatgctt ctgaaactga aagccgcctt cgtaaagccg actcggctca
     1861 tagcccatag tcttgaaatt catcgtgcgc tcttcggtca cagtgagcgg tgagtcggaa
     1921 tgaagtagca tctgaaaaga cgccttccgc tcaccctctc gatggtcctg aatacgcgcc
     1981 gcaatttcat cgcggagccg gaatcgatcg agcgccagag taccgatgtc cgcaatccca
     2041 tacttcgccg tcagcccccg aataaccttc cgaagaaact ctgccgattc cccgacggga
     2101 atgtcgtggt gatcgatttc ccgatccagc caggcggcga gcctttctaa tgaccagtcg
     2161 tcttgaccgc tgaattgaaa catctgttgg tgcagcgtgc cgacaaaatc ggcatcgcct
     2221 gtatcgccca gcaacgtcgt ctggacatca cccttctgtc cgacatcgat caccccaacc
     2281 tttccatacg gacgagccag cgggttatac gccgcaggca acgacgcatc tttctcgctc
     2341 agtttccagg ggtggtcgag caaaaaagtg ctctcgaact cgtacagcat gccgttctcg
     2401 gcaaagcaga gcagcggcac aagaaagtct aactgctgct catacggcga gggctttctc
     2461 ggctttccag ttccgccgaa ggcttgctcc gcctgacgga ccatctccgc ggcttccgcg
     2521 agcctggctt tggcgcccgg agtcgtgaca cagctttgca ccttttccag gtcatcgcgg
     2581 tcgagcggca cgactatggt gatggcgccg ctggcggcat cgatccgcac cttcccgcca
     2641 agcgcgggct cctggacctg gacgactgtg ggatcgatct cgtcaggacc aacggtcaca
     2701 gtctgcggtt gcacaccgag cggcagcgtg ccttgcggca cggaaagaat gatccgttcg
     2761 gcctctgcct tcgtaaaccc attgtgttcc aacgcctcgc gcagctccgc caataccgct
     2821 gtaatcgaat ccgagacaga gaaggcatag gcgcaattca gatcaggatg ttgcttcgcc
     2881 ttggcgttcg gcaaccggag gatgcgcccg acaatttgct caatcgccgt ggccgaccag
     2941 gtttctttca agctgcagag cacataggca aaggggcaat cccatccctc ccgaagtttc
     3001 tccaccgtga tgatcacccg aaccaggcac ttcggcgaga cgatatcctt cacccctttg
     3061 agttcatcga gcctgccgac ggagatcttc acctcgtcgt ttgaaagccc gaactcgcgc
     3121 accaaccgct cacgcagcgg ttcacaggca tcgacccgct cagcctgaat caagagaatg
     3181 ggacggatat attcggccgt ctgctgccct tccaggacgg cgagcctttc cagatcggtt
     3241 cgaagcgtga tggcttccgc cagcaggtgg tcccgctgac ttgggtgtct ggtgacaacc
     3301 cgcaacggca acttgaccat ctgcgccgtt ttgagttccg ccgccgagac acgatgcagc
     3361 acgttggacg gagtcttttc gcgtgccggc gtcgccgtaa actcgacgat gcacgagggc
     3421 aggacgttgc cgagcgtcgc aaaggacagg tcggtccggg cattgtgcgc ctcatccacg
     3481 atcacgatcg gccgccgaag ccgcagcgca ttgaccagcg atggcttggg cttcccgtcc
     3541 gcgccggaga ataggtccgc aaagcggtca ggaggcacat tcatcacatg ctcggaaaac
     3601 gcgccgttct ggtcatagac cctgcgccca gtcgtatcct caacacggaa cgattgaatg
     3661 gtggacacga tcaccaccgt ttgcccatcg atgacggcac gcgacaagcg caacgcctcg
     3721 tcgatggtca tcacttcgac cgccccgcaa gccaattcca gcgcacggcg gtacggatgg
     3781 cgtggatcgc gcaacgcatc cgccgtttga tccagaatcg tatggctcgg caccagccag
     3841 agcaccaccg ctcgctcggc ccgcatgaac tggcgctgag ccaatccggc cgcataacag
     3901 gcgagtaaag tcttgccgcc cccggtcggc acgcgcaaac agacataggg catgtccggc
     3961 ccaagccctg cagccgagac cggaaagtat ggcgcggcct caccgaatcg acgcgtgacc
     4021 tcccggaagg cgacctctgg ctttttggtc tgggcagtga ggcgaaagaa gtcgcctaac
     4081 gactccagca cccgctcttg ataatccttg agcgtaatca cgctttgccc ttcgactgaa
     4141 agccaatccg ccgtgtggga gcagccggag gcggctgcaa cagcggtaac agcttctcgt
     4201 atacatccaa caaggccgtg tcgtgcccca gcaaggtgcg atcgatttcg gccaaacgtt
     4261 tcagaatcgc ggcgttggct gcgatctgct cgcgcaggcg gacgaacgcc cgcaccacaa
     4321 acacgctcat ctggaccgcc cgctcgctac ggagaatgtt cgcggccatc acggcaccgt
     4381 gttcggtaaa agcccgcgga caataccgcc gcccgccgtg ggaggtggtc acaatctgtg
     4441 accacttcgg atgaacgccc gtcgattcag catgttgcaa acttgaggtc gcaatttgcg
     4501 acctcaagtt gctgaactca gcagtcgtca gttgaaaggc aaaatcatcg gggaaccgct
     4561 gtcgattccg cttgaatgcc tcgttgaagc gctttgtcgt cactccatag agccgtgcca
     4621 aatctgcgtc gagaatgatg cggtgccccc tgatgacaag gatcaagggt tccagagaat
     4681 ctagagc
//
LOCUS       DAORQN010000118         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_723_length_4594_cov_5.463318, whole genome shotgun sequence.
ACCESSION   DAORQN010000118 DAORQN010000000
VERSION     DAORQN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_4594_cov_5.463318"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PK614_06955"
     CDS             <1..656
                     /locus_tag="PK614_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB81987.1"
                     /translation="IEPVKRVAARVIEAAKRSKYSEMANQFEWEVTVIKDDKTMNAFA
                     LPGGKIAVYTGIFPVAKTEAGLAAVMGHEVVHALARHGGERMSQNTLAQTTLQAIGIA
                     LGVSGANPVVSQGAMAALGVGAQVGVLLPFSRKHESEADYVGVLLAADAGYDPRESIQ
                     LWTRMGELSGGKSASEFMSTHPSHETRIEQLEEWMAEAMPIYQSKPPAPNHELPALR"
     gene            717..1141
                     /gene="rnpB"
                     /locus_tag="PK614_06960"
     ncRNA           717..1141
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK614_06960"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1158..1430)
                     /locus_tag="PK614_06965"
     CDS             complement(1158..1430)
                     /locus_tag="PK614_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRB81988.1"
                     /translation="MPRDIGESADPLIVLPMNPLRVTIMSRHECHLCRVVTRVVRQVQ
                     QDLPFELITVDIDSDERLRKQYSDRVPVLWINDRETFSGKVTAGAA"
     gene            1635..3413
                     /gene="ilvB"
                     /locus_tag="PK614_06970"
     CDS             1635..3413
                     /gene="ilvB"
                     /locus_tag="PK614_06970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRB81989.1"
                     /translation="MKLTGSEILIECLKAEGVKTIFALPGGVVLKIFDMLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCISGQV
                     STSLIGNDAFQEADNIGLSRPCTKYNFLVKDVNDLATTIKEAFYIATTGRPGPVLVDI
                     PKDVSMAKTEYTYPSSVSIRGYNPTYEGNKWQIKQAAEAIMKAKKPILYVGGGVVFSQ
                     ASKELLELAEMTQIPVDMTLMGLGAFPGEHPQSMGMMGMHGTYCANMAVHYSDLVIAV
                     GARFDDRVTGKVSEFCPFAKVIHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILRA
                     TVNGNQKDLRKPWWDQIREWQQAHPLAYQQEPDGAIKPQHVVKRLYELTKDRDPIVAT
                     DVGQHQMWTAQYFKLARPNRWLTSGGLGTMGFGFPAAMGAQAAFRDRLVLCVAGDGSI
                     QMNMQEMAAAVVSKLPVKVIILNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAE
                     AYGAVGLRASKVGDLDTVLKEAIATDKPVVVDVPTYPYENCYPMIPAGGCNHEMILED
                     PPELKRRMAGAPSTGSDEDKDTILTA"
     assembly_gap    3535..3582
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3583..>3931
                     /gene="ilvN"
                     /locus_tag="PK614_06975"
     CDS             <3583..>3931
                     /gene="ilvN"
                     /locus_tag="PK614_06975"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRB81990.1"
                     /translation="RIVEQIVKQLNKLIDVIKVVDLNESEFVSRETALIKVHTKAEDR
                     AEALRIADIFRANVIDSTPSTYTIEVTGDPKKLEAIINLLQPLGIKELIRTGRVAIAR
                     EAIRPALSQPKKIA"
     assembly_gap    3932..3941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4016..>4594
                     /gene="ilvC"
                     /locus_tag="PK614_06980"
     CDS             4016..>4594
                     /gene="ilvC"
                     /locus_tag="PK614_06980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRB81991.1"
                     /translation="MKIYYDKDADLQLIRGKKVAVIGYGSQGHAHSLNLKESGANVVV
                     GLREGSSWKKAEASGLKVMPVADAVKASDVIMILAPDEAQAAIYRQEIAPNLKPESYL
                     AFGHGFNIHFGQIVPPADINVFMVAPKGPGHLVRSEYTKGSGVPCLLALHQDPSGNTR
                     QVGLAYASAIGGGRAGIIETNFREETETDLFGE"
BASE COUNT          996 a         1190 c         1424 g          926 t
ORIGIN      
        1 agattgaacc ggtgaaacgg gtggcggcgc gcgtgatcga agcagccaag cgttctaagt
       61 acagcgagat ggcaaatcaa ttcgagtggg aagtgacggt catcaaggac gacaagacga
      121 tgaacgcgtt tgcgctgccg ggcggaaaga tcgccgtcta caccggtatt tttccggtgg
      181 ccaagaccga ggccggactt gcggccgtga tgggccatga ggttgtgcac gcgttggcac
      241 ggcacggcgg ggaacggatg agtcagaata ccttggcgca gacgaccttg caggcgatcg
      301 gaattgcgct cggcgtgagt ggcgccaatc cggtggtttc gcaaggagcg atggcggcgt
      361 tgggtgtcgg tgcacaagtc ggggtgttgc ttccgttcag ccggaaacac gagtccgagg
      421 ccgactatgt cggcgtgttg ctcgcggcag atgccggata tgacccacgg gaatcgattc
      481 agctttggac gcgaatgggc gaactctccg gcggcaaatc ggcgtctgaa ttcatgtcga
      541 cccaccccag ccacgagact agaatcgagc agctggagga atggatggcg gaggccatgc
      601 cgatctatca gtcgaagcca ccggcgccca accacgagct tccagcgttg cgttagtgcc
      661 ggagggctcg ccgcttgaat ccctatcggg cggctcgtat actttcgttg cgcgctgggg
      721 ggacacgggt gctcgctcgt cgagcgtgct cgacgggagg aaagtccgga ctccaagggc
      781 aaggacgctg gataacgtcc aggcggagcg atccgacacc agcaccacag agaacaaacc
      841 gccgatggcc ggtgcgggag acttcggtcg agtgcgccgg ctcaggtaag ggtgaaacgg
      901 tggggtaaga gcccaccgcg gtgatggcga catcaacggc acggtaagcg tcgtccggag
      961 caagaccaaa tagggggacg cctgtgggct cgcgagagct cggcagaaag gccggcccgg
     1021 ccgaagttcc cgggtaggtt gcttgaggct cgaggtaact cgagtcccag agaaatgatc
     1081 acccccgtgt gagcgcaagc ttcacgcggg acagaatccg gcttataggg tttctccaac
     1141 gcgctttttt aatcgcttca cgcagctccg gcggtcacct tgccggagaa cgtttcccgg
     1201 tcattgatcc acagcaccgg tacgcggtcg ctgtactgtt tgcggaggcg ctcgtcactg
     1261 tcgatatcga ctgtgatgag ctcaaaggga aggtcttgtt gcacttgtct gaccacccgt
     1321 gtgaccactc gacaaagatg gcactcgtgg cgggacatga tggtgacgcg tagaggattc
     1381 ataggcagca cgatcaatgg gtcggctgac tcccctatat cacggggcat gagccggcac
     1441 cagtctcccc ggcgaagaat tcgtgcatcg gctctcttga cctcacttga gagctggtga
     1501 tacgatccgc catcttaccc gttgattttc cagaaagatt gtatcagtct gtgtcgcgtg
     1561 tgtgacgcag acagtcgagt gttctcagtt aaggcatcgc gtccttcgtc acgatgtgcg
     1621 aaggggaggg tgatatgaag cttacagggt cggaaatcct gatcgaatgc ttaaaagccg
     1681 aaggcgtgaa aacaattttc gccttgccgg gcggtgtggt gttgaagatt ttcgacatgc
     1741 tgcaccagca aaaagatatc gaagtcgtct tgacccggca cgaacagggc gcggggcata
     1801 tggccgaagg ctatgccaag gctaccggca aggccggtgt gtgtttggtg acgtccgggc
     1861 ctggtatgac caacgtgatt acggctctgg ccgatgccta tatggattcc gtgccgttgg
     1921 tgtgtatcag cgggcaggtc tcgacgagtt tgatcggcaa cgacgccttt caggaagccg
     1981 acaacatcgg ccttagccgg ccttgcacaa aatacaattt cctcgtgaag gatgtgaacg
     2041 atctggcgac gaccatcaag gaagcgtttt atatcgccac gaccggccgt ccaggaccgg
     2101 tcttggtgga tattcctaaa gacgtgtcga tggccaagac ggagtatacc tatcccagct
     2161 cggtctcgat tcgcgggtac aacccgacct acgagggaaa taagtggcag attaagcagg
     2221 ccgccgaagc catcatgaag gcgaagaagc ccattctgta tgtcggcggg ggcgtcgtct
     2281 tttcgcaggc atccaaagag ttgctcgaat tggcggagat gactcagatc cccgtggata
     2341 tgaccttgat gggcctcggg gcgttcccgg gcgaacatcc gcagtccatg ggcatgatgg
     2401 gcatgcacgg cacctactgc gccaacatgg ctgtccatta ttccgatctg gtcatcgcgg
     2461 tgggggcccg ctttgacgat cgtgtgacgg ggaaggtgtc ggagttctgc ccgttcgcga
     2521 aggtcattca catcgatatc gatccgacct ctatccggaa gaatatccat gtcgatattc
     2581 ccatcgtggg cgattgcaag gccgtcttac gggagctgaa tcaaattctc cgtgccaccg
     2641 tgaacgggaa ccaaaaggat ctgcgcaagc cctggtggga tcaaatccgc gaatggcagc
     2701 aggcgcatcc gttggcctac cagcaggagc ccgatggggc gattaaaccg caacatgtcg
     2761 tcaagcggtt gtatgaactg acgaaggatc gtgatccgat cgtggcgacc gatgtgggcc
     2821 agcaccagat gtggacggcg cagtacttca agcttgcccg accgaatcgc tggttgacct
     2881 cgggcggttt gggtaccatg gggttcgggt ttccggctgc tatgggggcg caggccgcgt
     2941 tcagagatcg attggtgctc tgtgtggccg gcgacggcag catacaaatg aatatgcagg
     3001 aaatggctgc cgcggtggtg tcgaagctgc cggtcaaggt aattattctg aacaatcgtt
     3061 tccacggcat ggttcggcag tggcaggacc tgttctacga aggacggtat gcctccagtt
     3121 atttggatac gacgccggat ttcgtgaagc tggccgaagc ctatggggcg gtcggtttgc
     3181 gcgcaagcaa ggtcggggat ctggataccg tgttgaagga agccatcgcg accgacaagc
     3241 ccgtcgtcgt cgatgtgccg acctatccct atgagaattg ttatccgatg attccggcgg
     3301 gcgggtgcaa tcacgagatg attctcgaag atccgcctga actgaagcga agaatggccg
     3361 gcgcgcccag caccgggtcc gacgaagata aggacacgat tttaaccgct taaatcacgc
     3421 tgtcagccga cagctatcag gatagagttt tccgatggaa catatcattt cggtcacggt
     3481 agaaaataag ttcggcgtgt tatcccgcgt ggccgggttg ttcagcggcc gcggnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacggatc gtggaacaaa
     3601 tcgtcaagca gctgaataag ctgatcgatg tcatcaaggt cgtggatctc aatgagagtg
     3661 aattcgtttc gcgggagacg gcgctcatca aggttcatac caaggcagaa gaccgagccg
     3721 aggcgctccg gatcgcagat attttccggg ccaacgtgat tgactcgaca ccctcgacct
     3781 acaccatcga ggtgacgggt gatccaaaga agcttgaggc catcattaat ctgctccagc
     3841 cgctcggcat caaggaactg attcgaacgg ggcgagtggc gattgcgcgt gaagcgatcc
     3901 ggccggccct cagccaaccc aaaaagatcg cnnnnnnnnn nggaataatt cgctcatccc
     3961 ctgtgtgcaa ctgtcgtttc cacccatgtg ccttatgaac cttcaggagt gatgtatgaa
     4021 gatctattac gacaaagatg ccgatttaca gctcattcgc ggaaagaaag tggccgtgat
     4081 cggctacggc agccagggcc atgcccattc gctcaatctc aaggaaagcg gcgccaatgt
     4141 cgtcgtgggt ttgcgtgaag gcagttcctg gaagaaggcg gaagcgagcg gactgaaggt
     4201 catgccggtg gccgatgccg tcaaagcctc cgatgtcatc atgattctgg ctcccgatga
     4261 ggcacaggca gccatttacc gccaggagat cgctccgaat ctgaagcccg agtcctattt
     4321 ggccttcggt cacgggttca atattcactt cggccagatt gtgccgcccg cggacatcaa
     4381 cgtcttcatg gtggccccca aggggccagg tcacctggtg cggtcggaat atacaaaagg
     4441 cagcggcgtc ccttgtttgc tggcattgca tcaggatccc agcggcaaca cgcgccaggt
     4501 gggtttggcc tacgccagcg ctatcggcgg cggacgggcc ggcatcattg aaacgaactt
     4561 tcgtgaagaa accgaaacgg atttgttcgg cgag
//
LOCUS       DAORQN010000119         4583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_725_length_4583_cov_5.554770, whole genome shotgun sequence.
ACCESSION   DAORQN010000119 DAORQN010000000
VERSION     DAORQN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_725_length_4583_cov_5.554770"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK614_06985"
     CDS             complement(<1..264)
                     /locus_tag="PK614_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81992.1"
                     /translation="MRTDRGWVSVLAMVFMIVLQATVGMAADLTSNDPRTMRFPTVEF
                     NPPDAERVVLENGMVVYLLEDHELPLVTINATLRTGSWLDPADK"
     gene            complement(321..1892)
                     /locus_tag="PK614_06990"
     CDS             complement(321..1892)
                     /locus_tag="PK614_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB81993.1"
                     /translation="MMSITGARTMGALILAGVLAAAVPGQAGSPSLADRVIEHKLANG
                     MTVLMVERHQAPIVSVNMTFGVGGVNEQVGQTGLAHLYEHMAFKGTRTVGTRDYEREQ
                     AVLDDLAMVGTELDRREREEAARAQAEGKPPVPSERVQQLQRRFKELQEKAGEYVVGN
                     EMALLYQRHGGVGLNASTGKDITRYVISLPANRLPLWAALESDRMAHPVLREFYKERG
                     VVMEERRLRTDDSPNGLLYETFTSTAFQAHQYGVPTIGWGSDILSLTPAATEAFFKTY
                     YGPNNATVAIVGDINPKEVIALIEQTFGKIPAAPPIPSLVTEEPPQRGERRVEIEFDA
                     EPALAIGYHKPTIGHPDDFVFDVIDEVLTEGVTSRLYSTLVRDKRLAASVLSDTNYPG
                     VRAPNLFVIAATPLAPHSVTEVETAIYEELDRLKTEPISAKEFERVLNGLDADLVRSL
                     RSNSGLASQLAFYQTVAGTWRYVLNARDRIAAVTPADVQRVAAQYLTKPNRTVGVLVK
                     KAQDKKMAAAGEVAR"
     gene            complement(1889..2539)
                     /locus_tag="PK614_06995"
     CDS             complement(1889..2539)
                     /locus_tag="PK614_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRB81994.1"
                     /translation="MTKKAEIDLLIFDLDGTLIESKWDIADSVNLTLRDLGVPERPQE
                     EIFGFVGDGVKKLLRLAVGEGNKDLYDEALRVFRGHYLEHCLDRTSFYPGIDQVLTHF
                     STKPKAVATNKAIEYTNVILKGLGPQHFVYVVGGDNGFGLKPEPGMLLHGMEQLGVEK
                     DRTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRQKMAACEPDWFIERPEELMELFI"
     gene            complement(2536..3099)
                     /locus_tag="PK614_07000"
     CDS             complement(2536..3099)
                     /locus_tag="PK614_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81995.1"
                     /translation="MNSTRRSVLITFIAAVLLLPASVTPGLAQTNAVSHSPAKVVEKY
                     FALDNKGVRLDAGSFEAVAGLVDWKEEPAWGKVIVINGFSVPDDFRQWEIVNRLEVLV
                     PVEFHVLGVMYLDTAGFVPEPGTEQVRVRVKVQGARWKIMEPILPPHVGQKRMLNVVR
                     QAMLEEKDGVRHESLAALQAELRKARE"
     gene            complement(3104..>4583)
                     /gene="pyk"
                     /locus_tag="PK614_07005"
     CDS             complement(3104..>4583)
                     /gene="pyk"
                     /locus_tag="PK614_07005"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRB81996.1"
                     /translation="ILSRQKNSAMRKAKIVCTIGPASDGAVVLGQLIRSGMDAARLNF
                     SHGTHDSHGRAIKTIRDTADRHGVAIAIIQDLQGPRIRVGKIDGVLELIAGRRVRLRT
                     MSLRSGGQIGARTVIAAGTVQDIPVTYQALTRDIRPGAKILIDDGLVELTADRIVDGA
                     VECTVVAGGRVTSHKGMNLPGTVVSAPTLTEKDREDLRFGVAQGVDYIALSFVRGAQD
                     IMAAKELIAECGGDVPVIAKIERQEAVTDLEAILVHADGVMVARGDLGVELGPEAVPV
                     LQKRIIATANRQRRLVITATQMLESMTQHLRPTRAEASDVANAVFDGTDAVMLSAETA
                     VGHYPVEVVQVMDRIIRAAEVETGPCFVRRSQGEQGQDSIPEAISLSAYSAAATIGAS
                     AIVAFSERGTTARLVSKQRPVAPIIGLTPFETVRRQMALYWGVLPHTVPQIPTTDERV
                     NEAERRLKAEGLARSGQRIVILSGTRIGQPGGTNLMKLHEVA"
BASE COUNT          871 a         1572 c         1234 g          906 t
ORIGIN      
        1 cttgtccgcc gggtccagcc agctgccggt tctcagcgtg gcattgatcg tcaccagcgg
       61 aagttcgtga tcttcgagca gatagaccac catgccgttt tccagcacca cacgctccgc
      121 atccggcggg ttaaattcca ccgtcgggaa ccgcatcgtg cgaggatcat tcgacgtcag
      181 gtccgccgcc attcctaccg tcgcctgcaa gacgatcatg aagaccatcg ccaggaccga
      241 cacccatcct ctgtctgttc tcattcgacg tcctccgcca tcttcacgtt tcacgagaga
      301 cgcttcacga atcacgcagg tcatcgggcc acctcacccg cagccgccat tttcttgtct
      361 tgcgccttct tgaccaggac accgaccgtc cggttcggct tcgtcaaata ctgggcagcc
      421 acccgctgca catcggccgg ggtcaccgcc gcgatccggt cacgcgcgtt cagcacatac
      481 cgccaggtac cggccacagt ctgataaaag gccaactgcg aggcgaggcc gctgttcgaa
      541 cgcagggatc gcaccagatc cgcatcgagt ccattgagca cccgctcgaa ctccttggcc
      601 gaaatcggct cggtcttcaa ccggtcaagc tcctcgtaaa ttgccgtttc cacttccgtc
      661 acactatggg gcgcgagcgg ggtcgccgcg atcacgaaaa ggttcggggc gcggacgccg
      721 ggataattcg tatcggacaa caccgaagcc gccagccgct tgtcacgcac gagtgtgctg
      781 taaagccggg acgtcacgcc ttcggtcaac acttcgtcga tcacgtcgaa tacgaaatcg
      841 tcgggatggc cgatggtggg cttgtggtaa ccgatcgcca acgcgggctc ggcatcgaat
      901 tcgatttcga cgcggcgctc accccgttgc ggaggctcct ccgtcaccag ggatgggatg
      961 ggaggcgcgg ccggaatttt tccgaaggtc tgctcgatca acgcaatgac ttctttcgga
     1021 ttgatgtccc cgacaatcgc caccgtcgcg ttgttcggtc cgtaataggt cttgaagaac
     1081 gcctccgtcg ccgccggcgt gagggacagg atgtccgatc cccagccgat ggtcggcaca
     1141 ccgtattgat gcgcctggaa cgccgtggag gtgaacgttt catagagcag gccgttcgga
     1201 ctgtcgtcgg tccgaagccg ccgctcctcc atgaccacgc cccgctcctt ataaaactcg
     1261 cgcaggaccg gatgcgccat ccggtccgac tccaacgccg cccagagcgg caaccggttg
     1321 gccggcagac tgatgacgta gcgtgtgatg tctttgccgg tcgaagcatt gagacccacg
     1381 ccgccatggc gctgatagag cagcgccatc tcgttgccca ccacatactc ccccgccttc
     1441 tcctgcaact ctttgaagcg ccgctgtagc tgctggaccc tctcggaggg aaccggcggt
     1501 ttgccttccg cctgcgcgcg ggccgcttct tcacgttcac gccggtcaag ctcggtgccg
     1561 accatggcca aatcgtccag gacggcttgt tcccgctcgt aatcccgcgt gccgactgtc
     1621 cgggtgccct tgaacgccat atgttcgtac agatgcgcca agccggtctg gccgacctgc
     1681 tcattcaccc cgccgactcc gaacgtcatg ttgacgctga cgatcggggc ctggtgccgt
     1741 tctaccatca agacggtcat cccgttggca agcttgtgct cgatgacccg gtcagccaga
     1801 ctcggggacc cggcctgacc cggcacggct gccgcaagca ctccggccag gataagcgca
     1861 cccattgttc tcgcccctgt gatgctcatc atatgaaaag ctccatcagt tcctccggtc
     1921 gttcaataaa ccaatcgggc tcacaggcgg ccatcttctg acggttgccc atgccatacc
     1981 ccaccgcaca gacccggatg cctgcattgt gaccgccgtt gatgtcgttg gtgctgtcgc
     2041 cgaccagcac cgtacggtcc ttctcgactc cgagttgttc catcccgtgc aacagcatgc
     2101 cgggctcggg cttgagaccg aacccgttgt cgccgccgac cacatagaca aagtgttgcg
     2161 ggccgaggcc cttcaaaatc acgttggtat attcgatcgc cttattggtc gccacggcct
     2221 tgggcttggt ggagaaatgg gtgagaacct ggtcgatccc cggataaaac gaggtgcgat
     2281 ccaggcaatg ttccagataa tgtccgcgaa agacccggag cgcttcatcg tacaaatcct
     2341 tgttgccttc gccgaccgcc agccgcaaga gcttcttcac gccgtcaccg acgaatccga
     2401 aaatttcctc ctgcgggcgc tccggcacac ccagatcgcg caacgtcaaa ttgacgctgt
     2461 cggcgatatc ccatttcgac tcgatcaacg tgccgtcgag gtcgaaaatc agcaaatcga
     2521 tttcagcctt cttcgtcatt ctcgtgcctt tcgcaactcg gcctgcaagg ccgccaacga
     2581 ctcgtggcgc actccatcct tctcttccag catggcctgc cggaccacat tcagcatccg
     2641 cttctggccg acatggggcg ggagaatcgg ctccatgatt ttccatcgtg ccccctgcac
     2701 cttcacgcgg acacgcacct gctccgtgcc cggttccggt acgaagccgg ccgtatccaa
     2761 gtacatgacg cccagcacat gaaactccac cggcaccaat acctccaacc tgttcacaat
     2821 ctcccactgg cgaaaatcat ccggcaccga aaagccgttg atgacgatga cttttcccca
     2881 ggccggctct tccttccaat ccacgaggcc cgccaccgcc tcgaaggaac cggcatcgag
     2941 ccgcaccccc ttgttgtcga gggcaaagta tttctccacc accttcgccg gagaatgcga
     3001 caccgcattg gtttgagcca ggcccggagt cacagaggcc ggcaacagaa gaaccgccgc
     3061 tatgaacgtg atgagcacgc tgcgccgtgt actgttcatg tgactaagcc acctcatgca
     3121 atttcattag attcgtcccg cccggttgcc cgattctggt gccggacaaa atgacgatgc
     3181 gttgtccgct ccgtgccaaa ccctcggcct tcaagcgccg ctcagcctcg ttgacccgct
     3241 catcggtcgt cggaatctgt ggcacggtgt ggggaagcac cccccaatac aacgccatct
     3301 gccggcggac cgtctcgaac ggcgtcaacc cgatgatcgg cgcaaccgga cgttgcttgg
     3361 aaaccagtcg ggccgtcgtc ccccgttcac tgaaggcgac gatcgcactc gcgccgatcg
     3421 tcgcggcagc cgagtaggcc gagagcgaga tggcttccgg aatagaatcc tgtccctgct
     3481 cgccctgcga gcgacggaca aaacagggcc ccgtttcgac ttcggccgcc cgtatgatcc
     3541 gatccatcac ctgcacgact tccaccggat agtggccaac ggccgtttcc gcggagagca
     3601 tcacggcatc ggtgccatcg aacacggcat tggccacatc cgacgcttcc gccctggtcg
     3661 gccgcaaatg ctgtgtcatg gactcgagca tctgcgtcgc ggtaatcacc agccgtcgtt
     3721 ggcgattcgc cgtcgcgatg atgcgcttct gcagcaccgg cacggcttcc gggccgagct
     3781 caacgcccaa atctccgcgc gccaccatga ccccgtcggc gtgaacaaga atggcctcca
     3841 ggtccgtgac ggcctcctgc cgctcgatct tggcgatcac cggcacgtcg ccgccgcatt
     3901 cggcaatcaa ttccttggcg gccatgatgt cctgcgctcc acggacaaag gataacgcga
     3961 tatagtccac accctgcgcc acgccgaaac gcaaatcttc ccggtctttc tccgtcaggg
     4021 tcggagcgct cacgaccgtg ccgggcagat tcataccctt atgagacgtc acgcgcccgc
     4081 ctgccaccac ggtacactct accgcaccat cgacgattcg atccgccgtc aattccacca
     4141 gtccatcgtc gatcagtatt ttggccccgg gccgaatgtc cctggtcagt gcctgatagg
     4201 tgacaggaat atcctgcacc gtacccgcgg cgatcaccgt cctggcaccg atctgcccgc
     4261 cggatcgtaa ggacatcgtc ctcagccgaa ctcgccgacc ggcgatgagc tccagcacgc
     4321 cgtcgatttt gccgacgcga atgcgcggcc cctgcaggtc ctgaatgatc gcaatggcca
     4381 ccccatggcg atcggccgtg tcgcgaatcg tcttgatcgc ccgcccatgc gagtcgtgcg
     4441 tgccgtggga aaaattcaaa cgggccgcat ccatgccgct tcgaatgagc tggccgagca
     4501 cgacggcgcc atcgctcgcc ggaccgatgg tgcagacgat tttcgctttt cgcatggcgc
     4561 tgttcttctg ccttgacaag att
//
LOCUS       DAORQN010000120         4527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_728_length_4527_cov_9.618962, whole genome shotgun sequence.
ACCESSION   DAORQN010000120 DAORQN010000000
VERSION     DAORQN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_728_length_4527_cov_9.618962"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK614_07010"
     CDS             complement(<1..479)
                     /locus_tag="PK614_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB81997.1"
                     /translation="MAHNTHPSAPDLLCTDLLQRLQFAILEHVTGTEFRIIGHPPAWL
                     FTLYPDARHNRQLSVDARTPVLQNFLADAADAWQTDGDDIAHSGFWSEQTAAEEPWHF
                     HAMALRHGPSRLLLIQQSTAAYDQQATILQRARAQVLPQHEHNRGPQRPQHALTTKLV
                     "
     gene            complement(469..1005)
                     /locus_tag="PK614_07015"
     CDS             complement(469..1005)
                     /locus_tag="PK614_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HRB81998.1"
                     /translation="MIEKKICMLGAFAVGKTSLVRRFVTSCFSEQYQTTIGVTVDKKT
                     MSMDGQPVTLVLWDLYGEDEFQKLRRSYLRGSSAYLVVLDGMRRATLDIALHIQLTAA
                     DTLGPVPFVVLINKLDRQTDWEVTDQDLAQLAQRGWTVLLTSAKTGQGVEEAFTVLAR
                     AMLATSEQTPPAGTTDGA"
     gene            complement(1019..2749)
                     /locus_tag="PK614_07020"
     CDS             complement(1019..2749)
                     /locus_tag="PK614_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB81999.1"
                     /translation="MAHAPKAGTPAVERDYAELRSLLLAPEQSRLEQLQEQVEHLDLN
                     AHNLNRVLPEAIALRGETDHRLTHALTPHVSEALGVSVRKQPHMIVDAIAPIMMPAIR
                     QAIANALRSMVQSLNQTIEHSLSIRSMQWRLEALRTGKPFAEIVLLHTLCYRVEQVFL
                     IHAQTGLLLAHAAGDAVAVQDQTLVSGMLSAIRSFVQDSFGATPDQALNTLQVGDLTV
                     WIEQGPSAILAAVIRGTPPETFHVHLQDTLDRIHAEHSDALTRFIGDAAPFTGTTPLL
                     EECLRAQFETRRRAIAPITWILLATIILAAIWWGVSAFQDRQHWQAYLDRLASEPGLV
                     VTSTHSAGNRYVLTGLRDPLAADPDVLLQGSGMAADRVEAKWSPYYALDAAFTLKRTG
                     IVLSPPDTVRLTLEGPRLTATGTASAEWIRQSRPLARLLPGITEYDDRRLVAQSLEAL
                     VQRMAGVWILFQQGTATIQSPEQLQGVRRISELLHQLDDTAHLSGATVILEITGQTDV
                     VGRSSRNQRLSEDRARSVLDALHPATFSAITFHARGIGPAPESSPSLGATTLPQDRRV
                     SFQATVHPTS"
     gene            complement(2866..3483)
                     /locus_tag="PK614_07025"
     CDS             complement(2866..3483)
                     /locus_tag="PK614_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82000.1"
                     /translation="MSRQTDLDRPRRSERTSGSSLADTDTESALTEEHAAGTNLDKIR
                     DILFGAQVRDHERRFAKLEAQLLAEAAQLRNDLKERFASLEQYIRKEVDTVTGRLTAE
                     EQDRTDAVGRLTTELQTLAGVLQQTATQLREQSEQAHHDLHTQLQNQATSLSGDFTQQ
                     HAALSATLDDAVRQLSHQKTDRASLATLFQELSQRLSHDQPPQQA"
     gene            complement(3616..4416)
                     /locus_tag="PK614_07030"
     CDS             complement(3616..4416)
                     /locus_tag="PK614_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB82001.1"
                     /translation="MSLANLVTQYIVQYGFRIMGAILVVVAALFVAKSVGRLFERWLN
                     EQDLEPPLRLLLLRLVKALVVILGLLIALDQLGLQIAPLVAGLGVAGLGMGLALQGVL
                     SNVVAGLSIIFTKPYRVGEHVSLLGVHGDVAKIDIFTTTLMHPDHSRIVIPNRKVVGE
                     ILHNFGTIRQLDLSIGVSYQTDIDTTLRVLRDLVTRHPKVLQDPAPMIGIAGFADSSI
                     TLSIRPWVQVAAVGTAHSELNQAIIQRLQADGVDIPFPQREVRLLNPS"
BASE COUNT          814 a         1249 c         1535 g          929 t
ORIGIN      
        1 accagtttcg tggtgagcgc gtgctggggg cgttgtggtc cacgattgtg ttcgtgctgc
       61 ggaagcacct gggcgcgggc ccgctggagg atggtggcct gttggtcgta ggctgctgtg
      121 ctttgttgga tcagcaagag acggctggga ccgtgtcgca gcgccatggc atgaaagtgc
      181 cagggctcct cggccgcggt ctgctcgctc caaaacccag agtgcgcgat gtcgtcgccg
      241 tctgtctgcc atgcatcggc tgcatcggcc aggaaattct gaagcaccgg tgtgcgagca
      301 tcgacggaca gttgccgatt gtgtcgggca tcggggtaga gggtgaatag ccaggccggc
      361 gggtgcccga tgatgcggaa ctctgtaccg gttacgtgtt cgaggatggc aaattggagc
      421 cgttgcaaca gatccgtgca gagtaggtcc ggtgcagacg ggtgggtgtt atgcgccatc
      481 ggttgttccc gcgggaggtg tttgttctga tgtggcgagc atggcgcggg cgagaactgt
      541 gaaggcctct tcgacaccct gccctgtctt ggcactggtc aggagcaccg tccaaccccg
      601 ttgggctaac tgcgcgaggt cttggtcggt cacttcccaa tccgtctggc ggtcgagttt
      661 attgatgagg accacaaacg gaaccgggcc gagtgtgtcc gccgcggtca gttgaatgtg
      721 caacgcgatg tccagggtgg cccgccgcat tccgtccagg actaccaggt acgcagacga
      781 tccgcgcaga tacgagcggc gtaatttttg gaattcgtct tctccgtaga gatcccagag
      841 caccaacgtc acgggctggc cgtccatcga catggttttc ttgtcgacgg tcaccccgat
      901 agtcgtttga tactgttcgg agaaacagct ggtgacgaag cggcgcacca ggctggtttt
      961 tcccacggca aaggcgccca gcatgcagat ttttttttcg atcatgtcag gggcgtcttc
     1021 atgaagtggg gtgaacagtg gcctgaaaag acactcgtcg gtcctgcggc agcgtggtgg
     1081 cacccaggga gggtgacgat tcaggtgccg ggccgatccc gcgagcgtga aacgtgatcg
     1141 cggaaaaggt ggcgggatga agggcatcga gcaccgaccg ggcgcgatcc tcgctcagcc
     1201 gttggttccg acttgaccgg cccacgacgt cggtttggcc ggtgatctct agtatcaccg
     1261 ttgcgccgga gagatgcgcc gtatcgtcca gctgatgcag gagctctgat atgcgacgca
     1321 ccccttgaag ttgttccggt gattggatcg tcgccgttcc ctgttggaac agaatccaga
     1381 cccctgccat ccgttggacc agtgcctcaa gcgattgagc gacgaggcgg cggtcgtcat
     1441 attcggtgat accggggagc aggcgcgcca gcggtcggct ctgccggatc cactctgcgc
     1501 tggctgtgcc ggtggccgtg aggcgcggtc cttcgagggt caagcgaact gtgtctgggg
     1561 gtgagaggac gattccggtt cgtttcaggg tgaacgcggc gtcgagggcg tagtaggggc
     1621 tccatttcgc ttcgactcga tcggcggcca tcccactgcc ctgcaacaac acgtccggat
     1681 cagcggccaa cgggtcgcgc aacccggtga gcacataccg attgcctgct gaatgcgtgg
     1741 aggtgacgac gaggcccggt tccgaggcca gccggtccag ataggcctgc cagtgttggc
     1801 ggtcttgaaa ggccgacact ccccaccaga ttgctgccag gataatcgtc gccaggagga
     1861 tccacgtgat gggcgcgatg gcacgccggc gcgtttcaaa ctgggcccgc aggcactctt
     1921 ccaggagcgg tgtggtcccg gtaaagggtg ccgcatcacc aatgaagcgc gtcagcgcat
     1981 cggaatgttc ggcgtgaatg cgatcgagcg tgtcttggag gtgaacgtga aacgtctccg
     2041 gtggcgtgcc acgtatgacg gccgcgagga ttgccgaggg gccctgttcg atccagacgg
     2101 tcagatcacc gacttgcaag gtgttcaacg cctgatcggg cgttgcgccg aacgaatcct
     2161 ggacgaagct ccgaatcgcc gacaacatgc ccgagaccag ggtctgatcc tgcacggcga
     2221 ccgcgtcgcc ggctgcgtgc gcgagcagca ggccggtctg tgcgtgaatg agaaagacct
     2281 gctcgactcg ataacagagg gtatgtagca ggacgatttc ggcgaacggt ttgccggtac
     2341 gcaaggcctc cagccgccac tgcatgcttc ggatcgagag gctgtgttca atggtttggt
     2401 tgagcgactg caccatgctg cgcaaggcat tggcgatggc ctggcggatc gccggcatca
     2461 tgatcggggc aatcgcatcg acgatcatgt ggggctgttt gcgcacggag acccccagcg
     2521 cttcggagac gtgaggggtg agtgcatggg tcagccggtg gtcggtctcc ccacgaagcg
     2581 cgatcgcctc gggaagcaca cgattcagat tatgggcgtt gaggtcgaga tgttcgacct
     2641 gctcctgcag ttgctccaga cgcgattgct cgggcgccag gaggaggctg cgcaattcgg
     2701 catagtcgcg ttcgactgcc ggcgtgccgg ctttaggtgc gtgcgccatc ccgtctccgt
     2761 ggcatgaagg aaggagggta ttctacgcga cacatcgttg tcgggaaaac agccgtacgc
     2821 gcggtctgaa gggcagaccg gaaccgttga cgaatcggcg tggagttagg cctgctgcgg
     2881 aggctggtca tgcgagagtc tctgtgagag ctcctggaac aacgtcgcaa gggacgcgcg
     2941 gtcggtcttt tggtgtgaga gttggcggac cgcatcgtcg agggtggccg ataaggcggc
     3001 gtgttgctgg gtgaaatcac cggagaggga tgttgcctgg ttctggagct gggtgtgcaa
     3061 atcgtgatgc gcctgctcgc tctgctcccg cagctgggtc gccgtctgct ggagcacgcc
     3121 ggccaacgtc tgcagttccg tggtcaggcg tccgaccgca tcggtgcggt cctgttcttc
     3181 ggcagtcagg cggcccgtca ccgtgtccac ttcttttcga atgtattgct ccagcgaggc
     3241 gaatcgttcc ttgaggtcgt tgcgcaattg ggctgcttct gccagcagct gagcttcgag
     3301 tttggcgaac cggcgctcat gatcacgtac ctgggcgccg aacagaatgt cgcggatttt
     3361 gtcgaggtta gtgccggctg catgctcctc ggtcagcgcg gattctgtgt ctgtgtcggc
     3421 gagactgctg cccgaggttc gttctgagcg tcgggggcga tcgaggtccg tctgcctgct
     3481 catgatgggg tctccttggt cgctcgttct atcacgaaag gggcctagac tagcatgttg
     3541 cgccggggtc aacaagtcag gccgggtgcc ggcggcgtgg atgtgcagcc tggagggtga
     3601 tcagcggctc tggccttagg atggattaag tagtcgtacc tcacgctgag ggaaggggat
     3661 gtccacccca tcagcttgta atcgttgaat gattgcctgg ttgagttcgc tgtgagccgt
     3721 tcctacggcc gcgacctgga cccagggccg aatggacagg gtaatggagg aatcggcgaa
     3781 gccggcaatc ccgatcatcg gggccgggtc ctgaagtact ttggggtgtc gggtgacgag
     3841 gtcgcgcagc acgcgtagtg tggtgtcgat atcggtttgg taggacaccc cgatggagag
     3901 atccagctgc cgaatcgtgc cgaagttgtg cagaatctct ccgacgactt tgcgattggg
     3961 gatcacgatc cgggagtgat ccggatgcat gagcgtcgtc gtgaagatat cgatttttgc
     4021 gacatccccg tgcaccccta agagcgacac atgttcgcca acccggtagg gtttggtgaa
     4081 aatgatcgag agaccggcca cgacattgct caagacgcct tgcagggcga ggcccatccc
     4141 caagccggcc acgccgagcc cggcgaccag cggcgcgatt tgtaggccga gttgatccag
     4201 cgcgatgaga agcccgagga tcaccacgag ggccttcacc agacgcagca gcagcagacg
     4261 gagtggtggc tccaggtcct gctcgttgag ccagcgctcg aagagtcgcc ccaccgattt
     4321 ggcgacgaac agggcggcca cgacgaccag gatcgccccc atgattcgaa agccgtattg
     4381 cacgatatat tgagtgacca gattggccaa cgacatgcgt cctccccgca actgttaccg
     4441 cccgaagagc cccttcaaga ggtccttgcc ttgctgtttg agatcgtccg gtttcgcgga
     4501 acctttcaag aggtctccca cggcgcc
//
LOCUS       DAORQN010000121         4434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_744_length_4434_cov_10.289792, whole genome shotgun sequence.
ACCESSION   DAORQN010000121 DAORQN010000000
VERSION     DAORQN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_4434_cov_10.289792"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK614_07035"
     CDS             complement(<1..266)
                     /locus_tag="PK614_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HRB82002.1"
                     /translation="MTMWPDGFTTRYRIAKDTLILDLGTRMRVLSSAPRGGGLRTTRY
                     IVNHQVPSNPVRQATHSKWEHPARYLLRIAVDLGVDPDCVGLMTA"
     gene            complement(263..1372)
                     /gene="cobD"
                     /locus_tag="PK614_07040"
     CDS             complement(263..1372)
                     /gene="cobD"
                     /locus_tag="PK614_07040"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HRB82003.1"
                     /translation="MTRIGHGGDVYAAARELGRDLHRLLDFSASINPLGPSPAAVRTL
                     RTAEVLLGHYPNPACWALRQALAAYWKRPSEEFLVGNGSTELIHLLPRTLGIRHLLLI
                     GPTFSEYAEAMTRAGGRVTMLMADRADGYRPPLESVLATLQKGRQSASGAYPIDAVLL
                     CNPNSPTGQACEAAAVRKCARLVARRGLWCIVDESFAEYANHASILSEPLPPRTVVLR
                     SFTKFYGLPGLRVGYATAKPAVIARIAAQQPPWSVNMLAQRTAVVALNDVQHAGRSLR
                     FMERERTRVQKALTQLPGCTVFPSAANFLLIEVPVGYKATAVVAALRRQGLLIRDCSQ
                     VPGLNDRSVRVAVRPRADNDRLLRALSVLLCKGGA"
     gene            complement(1369..2325)
                     /gene="cbiB"
                     /locus_tag="PK614_07045"
     CDS             complement(1369..2325)
                     /gene="cbiB"
                     /locus_tag="PK614_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRB82004.1"
                     /translation="MTGGELVLAAALDVVAGDPRWLPHPVRGMGVVISWFDDQVRTFC
                     RSDQSLQIAGACLALGLPAAVYAAATWLIAQAAAFSPLLGQLVGIGLAYTTLAGRDLF
                     DHVQAVSRELQQGNLAGAREAVARIVGRDSATLEEPEIVRATVETIAESTADGIIAPL
                     VYLSLGGAPLALAYKAVNTLDSMVGHHDERYEHFGWASARLDDLLNWVPARLTGGFIA
                     LASGLATAQWHRVQDSWYILHRDGDKHPSPNSGMPEAAMAGGLGVQLGGRNYYDGVAH
                     EGELIGDAGTALTPGHIDQATRIMVVAAGLGLFFALLSLWVS"
     gene            complement(2322..3128)
                     /locus_tag="PK614_07050"
     CDS             complement(2322..3128)
                     /locus_tag="PK614_07050"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRB82005.1"
                     /translation="MQHKSCDDSRVSHFTHDVFRPFILAWHFLTAIPISRSHHEPSSA
                     ELATSMAWYSTVGLLIGGFLAAADQGLHLFLAAEVVNVLLIVLLVLMTRGLHQDGLAD
                     TLDGLAGGRTAADRLRIMRDPSVGALGATGLFLSLLLRYAGLLALPQPVRLPVLLCMP
                     ALGRWAMVSVAWASPYARSEGGLAAAFLTHLSWQHVLLSSLVLACALMAGLGVIAAAA
                     TIGLGALVVVVVRRGCRAWFGGVTGDLLGATNELIEILFLLLIPVLVMAR"
     gene            complement(3211..4266)
                     /gene="cobT"
                     /locus_tag="PK614_07055"
     CDS             complement(3211..4266)
                     /gene="cobT"
                     /locus_tag="PK614_07055"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRB82006.1"
                     /translation="MTLQEVLTRIQPVDQAIAAQTQARLDRLTKPLGSLGRLEETAVR
                     YATITGEVKPAVPRGVVFTFAADHGVATEGVSAYPREVTPQMVLNFLRGGAGVNVLAR
                     HAGVEVRVVDIGVAYDFGSVSGLIHQKVMEGTNNLLVESAMSRDQAVQALQVGIGLAT
                     EAAREGVGLIGTGEMGIGNTTPSAAITAVMTGQPVTEVTGRGTGIDEAGHARKVGVIE
                     QALDRHRPNRADAIDVLAKVGGLEIAGLAGLILGAAAARVPVVLDGFIAGAAALIAVG
                     LQPLCRDYLIASHRSVERGHQAILEHLSLKPLLDLDLRLGEGTGACLGMSLVQASIKI
                     LTEMTTFEEAGVSQREA"
BASE COUNT          934 a         1518 c         1244 g          738 t
ORIGIN      
        1 gccgtcatca ggccgacaca atcaggatcc actcccaaat ccaccgcgat tcgcaggagg
       61 taacgggcag gatgttccca cttcgagtga gtcgcctgcc gaacaggatt ggagggcacc
      121 tgatgattca ctatgtagcg cgtcgttcga agccctcccc ctcttggagc ggacgacaac
      181 acccgcatgc gggttcccag atcgaggatg agcgtgtcct tggcgattcg gtagcgagtg
      241 gtgaaaccat ccggccacat cgtcatgcgc cacccttgca aaggagaacc gacagggctc
      301 gcaggaggcg atcattgtcg gccctgggac gcactgcgac gcgcaccgaa cggtcattca
      361 atccaggcac ttgcgaacaa tcccttatca ggagtccctg ccggcgaagc gcggccacca
      421 cggcggtcgc tttgtacccg accggcacct ctatcagcag aaaattggcg gcagagggaa
      481 agaccgtgca accgggcagc tgggtcagtg ccttctgaac gcgggttcgc tcccgttcca
      541 tgaaacgaag gcttcgcccg gcgtgttgca catcgttcaa ggcgacgacc gcagttcgct
      601 gcgccaacat gttcaccgac cagggcggct gctgcgccgc gattctggca atcacagcag
      661 gcttcgccgt ggcatagccc acacgcagtc cgggcaaccc ataaaacttg gtaaagctgc
      721 gcagcacgac ggtccgtggg ggaagaggct ctgacaagat cgacgcatga ttcgcatatt
      781 ccgcaaagga ttcatccacg atacaccaga gtccccggcg agccaccaga cgcgcacact
      841 tccttaccgc ggcagcctcg caggcctgac cggtcgggct attgggattg cagaggagta
      901 ctgcatcaat gggataggcg ccgctcgcag actgccgccc cttctgcaac gttgcgagga
      961 ccgactccag cggaggacga tatccgtcgg cgcgatccgc catcagcatg gtgacccgtc
     1021 ccccggcgcg tgtcatcgct tcagcatatt cggagaaggt cgggccgatc agcaggagat
     1081 gtctgatccc caatgtcctg ggaaggagat ggatcaactc ggtcgatccg ttgccgacca
     1141 aaaactcctc actcgggcgt ttccaatacg cggccagcgc ctgccgcagt gcccaacaag
     1201 ccgggttcgg ataatgaccg agcaacacct cggctgtccg cagggttcgc actgcggcgg
     1261 gagacggtcc caagggattg atgctggcgc tgaaatccag gagtcgatgg agatctcgcc
     1321 ctaactcacg ggccgccgca tatacatccc cgccatgacc gatgcgcgtc atgataccca
     1381 tagggagagc agtgcaaaga agagccccag tccggcagcg accaccataa tgcgagtggc
     1441 ctgatcgatg tgcccgggcg tcaacgcggt gccggcatca ccgatcaatt caccctcgtg
     1501 agccacaccg tcgtaataat tccgcccgcc gagttgcacg cccaatccac cggccatggc
     1561 cgcttccggc atgccgctgt tcgggctggg atgtttgtcg ccgtcccgat gcaggatgta
     1621 ccaactatcc tgcacacgat gccactgagc agtcgccagc cccgaagcca acgcgatgaa
     1681 gccgccagtc agccgggcag gcacccaatt cagcagatcg tccaatcggg cagaggccca
     1741 gccgaaatgt tcataccgct catcgtggtg tcccaccatc gaatcgagcg tattcaccgc
     1801 cttgtaggcc agcgcgagcg gcgcgccgcc aagggagagg tagacgagcg gcgcaataat
     1861 gccgtcggcg gtgctttcgg cgatcgtctc caccgtggcg cgcacgattt ccggttcctc
     1921 caacgtggcg ctgtcccggc cgacgatcct ggccacggcc tcacgcgctc cggcaagatt
     1981 cccctgctgc agctcacgac tgactgcctg gacatgatcg aagaggtccc ggcccgccag
     2041 tgtggtatag gccaggccga tgcctaccag ttgtccgagt agcggagaaa atgcagccgc
     2101 ctgcgcaatc agccaggtgg ccgccgcata gaccgccgcc ggtaacccca gcgcgagaca
     2161 ggccccggcg atttgtaagg attgatcact ccggcagaat gttctgacct gatcatcgaa
     2221 ccaggaaatg accacgccca tgccccgcac cggatggggc aaccaacggg gatctcccgc
     2281 cacgacatcc agcgccgccg ccagcaccaa ctcgccgccg gtcatcgcgc catcaccaac
     2341 accgggatca acaagaggaa gagaatttcg atcaattcat tcgtggcacc caacagatct
     2401 cccgtcactc cgccgaacca tgcccgacag ccccgccgca caacgaccac caccaaggct
     2461 cccaacccga tggtcgccgc tgccgcaatc actcctaatc cggccatcag cgcacaggcc
     2521 aacacgagac tcgaaagcaa cacatgctgc caggagagat gagtcaaaaa cgcggccgct
     2581 agcccgcctt cgctcctggc atagggagac gcccaggcga ctgagaccat cgcccaacgg
     2641 cccagcgccg gcatacagag cagcacggga agacgaaccg gttgagggag ggctagcaat
     2701 ccggcatacc gcaacagcag cgagagaaaa agaccggtcg cacccagtgc cccgacgctc
     2761 ggatcgcgca tgatgcgcag ccgatcggcc gcggttcgcc cgccggccag tccatctaaa
     2821 gtgtccgcca gcccgtcttg atgcagcccg cgtgtcatga gcaccagtag cacgatcagc
     2881 agaacattca ccacctcggc cgccaaaaac aagtgcaagc cctggtcagc cgccgcgaga
     2941 aatccgccga tcagaagccc gaccgtggag taccaggcca tcgacgtagc caactccgcg
     3001 gacgacggct catggtggct ccggctgatc ggaatcgccg tgaggaaatg ccaagccagg
     3061 atgaacgggc ggaaaacgtc atgcgtaaaa tgtgaaacgc gagaatcatc gcaactctta
     3121 tgctgcatgc gggtccggga ttctgccgga tgcgaacgtg ccgtgtctac ggttccctcg
     3181 cttggcattt cacgcttcac gtttcacctg tcacgcctca cgttgcgaga cacccgcttc
     3241 ttcgaacgtc gtcatctcgg tgagaatctt gatcgacgcc tgcacgaggc tcatcccgag
     3301 gcaggctcca gtcccttcgc ctaaccgcag atcgagatcg agcagcggct tcaagctcag
     3361 atgctccaga atcgcctgat gaccacgttc caccgaacgg tgcgaggcga tcagatagtc
     3421 ccggcagagc ggctgcaacc ccaccgcgat caacgccgca gcgccggcaa tgaacccatc
     3481 caacaccaca gggactcgtg ctgccgccgc acccaggatc agcccggcca agccggcaat
     3541 ctccagaccg ccgaccttcg cgagcacatc gatggcgtcg gcccggttcg gacgatgccg
     3601 gtcgagcgcc tgctcaatga cgccaacttt gcgggcgtga cccgcttcat cgatgccggt
     3661 tcctcgaccc gttacctcag tcaccggctg tccagtcatg actgctgtga tcgctgcact
     3721 cggcgtcgtg ttgccgatgc ccatctcgcc ggtcccgatc agcccaaccc cttcacgcgc
     3781 ggcctccgtg gccaacccga tcccgacctg caacgcttgc acggcctggt cacgactcat
     3841 ggctgattcc accaggagat tgttcgttcc ctccatcacc ttctggtgaa tcaggccgga
     3901 gaccgaccca aagtcgtacg cgactccgat atccaccacc cgcacctcga ctccggcgtg
     3961 acgcgcgagc acattcacgc cggcgccgcc acgcaggaaa ttcagcacca tttgcggcgt
     4021 gacttcgcgc ggataggcgc tcaccccttc agtcgccaca ccatggtcgg ccgcaaacgt
     4081 aaacacgact ccgcgcggca cagccggttt cacttcgccc gtgatcgtgg cataacgcac
     4141 cgcggtttct tccagccgcc ccagactacc gagaggtttg gtcaaccggt ccaggcgcgc
     4201 ttgcgtctgc gctgcgatcg cctgatccac cggttgaatg cgtgtcaaca cttcctggag
     4261 cgtcatcggg tcttctcccc tgtcatttga gtttgagcga ctggccgctg acaacaaagt
     4321 agacttcgtc tgccgctgcc gcgatctgtt ggttgacccg accggcgaca tcacggaagg
     4381 cgcgcgcgct gcgactggtc ggtaccaggc caaaccccag ctcattgctc acca
//
LOCUS       DAORQN010000122         4427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_745_length_4427_cov_63.273788, whole genome shotgun sequence.
ACCESSION   DAORQN010000122 DAORQN010000000
VERSION     DAORQN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_4427_cov_63.273788"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK614_07060"
     CDS             complement(<1..186)
                     /locus_tag="PK614_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82007.1"
                     /translation="MNMLVGRKARTWVLGLALLAGGGGGALGEAQAGTTDDLMDTTKH
                     PVSVTMAMQADSFFGFNP"
     gene            complement(323..2092)
                     /locus_tag="PK614_07065"
     CDS             complement(323..2092)
                     /locus_tag="PK614_07065"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRB82008.1"
                     /translation="MRSLRIAMAQINPTVGDIVGNTRLIQTWIKEARQAKADLVAFPE
                     LAITGYPPEDLLFKPRFIEDTHRALKAVAAEARGLVVVVGYVGQGATAVSASDASSFP
                     LSGRHELYNEAAVLSDRRILATYRKRHLPNYGVFDESRYFHPGSRLPLLVLNGATIGV
                     NICEDIWFSDGPTRAQAAAGAEVIVNINASPFHVGKGRMREQVLATRARENRVIVTYT
                     NTVGGQDELVFDGCSMIVDQAGEIVGRGKGFDQDLIVADLDVAAVGRARLAQGRKKPL
                     PPRVAALIDRVDVRLPARKSQSVVVPDLEPPLGRLDEAYRALVLGVQDYVRKNGFKRV
                     VIGLSGGVDSAITAVIAVDALGAENVLGVFMPSPYTSRASREDVADLASRLHIQVDTL
                     SITATFKSYLRALSRSFKGRRPDTTEENLQARIRGNLLMAYSNKFGHLVLTTGNKSEM
                     SVGYATLYGDMAGGFAVIKDVPKTMVYELSYMRNQVGPAPVIPKRVLERAPTAELRPD
                     QKDEDSLPPYAILDPILKAYVEEDRALEDIAAMGFDRETVARVIALVDRSEYKRRQAP
                     LGIKITHRAFGKDRRMPITNGYR"
     gene            2421..3323
                     /locus_tag="PK614_07070"
     CDS             2421..3323
                     /locus_tag="PK614_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82009.1"
                     /translation="MIRRPTPTQSRLSSFRLAWQLALPCLLLCACSLGTNLTPVGQGP
                     LGAVALERQTSRGTTAKYGTPQTFQASHPTHLTTSLAGRLLTGLSVSGLDRPGSSMAQ
                     ESYPLFSHEEVEFLAPLIVGALAQAQPDQRVRFTMQDDGLTTQGTLYVYKTTLRVNLS
                     HYRSASGDGQTKPASLILSFTPPQALVRADVPQPWMTVEPEQPRIAVSLEALSQLPAP
                     SPAAADHNTTAEAAPTQTSPTAEQSRLQQELQSTKDVVVKQAEELQKLKAELESVRRQ
                     LAEKDAAASKAKPKAAPRKPTATP"
     gene            complement(3340..3729)
                     /locus_tag="PK614_07075"
     CDS             complement(3340..3729)
                     /locus_tag="PK614_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82010.1"
                     /translation="MAKSRRVATAFLLTLMTSACATGQYTPLVGADRDEVEQLKENVY
                     RVEYRVSSFTSQEQLDAYLRRRCAELTLREGYDFFHLAQRADVLALSRRTSMTVTMYK
                     GKKPVGADDVYDAKEVLAQPDLLSGSQ"
     gene            complement(4027..>4427)
                     /locus_tag="PK614_07080"
     CDS             complement(4027..>4427)
                     /locus_tag="PK614_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HRB82011.1"
                     /translation="DMAAKEGLCSLLCVPMLVREKAIGVINTYTSKPHTFTAEDVKLM
                     QAIANQAAISIEHTTLLEKSFEMQEALQVRKLLDRAKGYLMRSKRLSEEDAFKLIQRQ
                     SMDLRKSMREIAEAILLAGEIEERADKGRS"
BASE COUNT          910 a         1477 c         1198 g          842 t
ORIGIN      
        1 gggattaaac ccgaagaaac tgtcggcttg catcgccatg gtcaccgaca cggggtgttt
       61 ggtcgtatcc atcaggtcgt ctgtcgttcc ggcttgcgcc tcgccgagcg ccccacctcc
      121 gccccccgca agtaatgcca accccaacac ccatgtccgt gcctttcggc caaccagcat
      181 attcatgacg cctccaaaga gagaagaagt aaaagtgccc gctcagaaga ccgtcgtaca
      241 gaacggtcta tgagccccgt ctctctggcg ccattgccag tgttgccgac cgacgtcgtt
      301 gtcggtcctg atgcggcccg tactaccggt acccgttggt gatcggcatc cgccgatcct
      361 tcccgaacgc tcgatgcgta atcttgatcc ccagcggcgc ctgccgccgc ttgtattcac
      421 tccgatccac cagcgcaatc acccgcgcga ccgtctcccg atcgaacccc atcgctgcaa
      481 tgtcttccaa cgcgcggtcc tcctcgacgt aggccttgag aatcggatcg aggatggcgt
      541 agggcggcaa actgtcttcg tccttttgat cggggcgtaa ctcagcggtc ggagcccgct
      601 caagcactcg cttcggaatc accggggccg gcccgacctg attgcgcatg tacgacagct
      661 cgtagaccat ggtcttcggc acatccttaa tcaccgcaaa ccctccggcc atatcgccgt
      721 acagcgtcgc gtatccgacg ctcatttcac tcttattgcc ggtggtcagc accagatgac
      781 cgaacttatt cgaataggcc atcagcagat tgcccctgat ccgggcctgc agattctctt
      841 cggtcgtgtc cgggcgccgt cccttgaagg atcggctgag ggcgcgcagg taactcttga
      901 acgtcgccgt aatggacagg gtatcgacct gaatgtgcag ccggctggcc agatcggcga
      961 catcttcgcg gctggctctg gacgtatagg gcgagggcat gaacactccg agcacattct
     1021 cagcgcccaa ggcgtctacc gcgatgaccg cggtgatggc cgagtccacc ccgccgctca
     1081 atccgatgac gacgcgcttg aacccgttct tgcggacata gtcctgcacc ccgaggacca
     1141 aggcgcgata ggcctcgtcg aggcgaccca gcggaggctc cagatccggc accacgaccg
     1201 attgcgactt tctcgcaggc aagcgcacat cgacccgatc aatgagggcc gccacacgcg
     1261 gcggcagggg tttcttgcgg ccctgggcca gtcgcgcccg ccccacggcc gcgacatcga
     1321 gatccgcaac gatgagatcc tggtcaaacc ccttcccccg ccccacgatc tcgccagcct
     1381 ggtccacaat catgctgcag ccgtcgaaaa ccaattcatc ctgcccgccg accgtgttgg
     1441 tgtacgtcac gatcacgcga ttttcacggg ccctggtcgc cagcacctgt tcgcgcatcc
     1501 gtcccttccc gacatggaac ggggatgcgt tgatattgac gatgacctcg gcccccgctg
     1561 cagcctgcgc acgggtggga ccgtcagaaa accagatgtc ttcgcaaata ttgacgccga
     1621 tcgtcgcgcc gttcaggacc agcagcggca gacgggagcc ggggtggaaa taccggctct
     1681 cgtcgaacac gccgtagttc ggcagatgcc gtttccggta ggtggccaga atccggcgat
     1741 cggacagcac cgccgcctcg ttgtataact catgccgacc ggacagcggg aacgaagaag
     1801 cgtccgacgc cgaaacagcc gtcgccccct gcccgacata cccgacgacc acgaccaacc
     1861 cccgcgcttc cgccgcgacc gccttcaatg cacgatgcgt gtcctcgatg aaacggggct
     1921 tgaacaacag atcctccggc ggatagccgg tgatggccag ttccgggaag gccacgaggt
     1981 cggccttggc ttggcgcgct tccttgatcc acgtctggat caagcgcgtg ttgccgacaa
     2041 tatccccgac ggtcggattg atctgggcca tggcaattcg gagcgaccgc atatctaaag
     2101 cctgtaagcc ctcagcgttc agcaatcagc ctgaacacga gtcatcggaa ctttaagatc
     2161 ctgagctgac gggattcatc agcatgctga taaaaaaaac gtcctcagac ccacctcggt
     2221 ggatcggagg acgtcgttgt cctgcttcca atgtatcgtt cggaacgaat tactcgtcgg
     2281 agacgccatt gtccccggaa attatcccgt gtgtatacca ccggcctaaa caggctgtca
     2341 accaattgaa tcgcactttt ccaggggaaa ccaggcggtt ccgcggtcag cctgtggtac
     2401 aatcggccgc catgcggctc atgattcgcc gtcccacccc aacacaatca cgactcagca
     2461 gtttccggct cgcctggcag ctggcgcttc cctgcctcct cctctgcgcc tgcagcctcg
     2521 ggacgaacct gactcctgtc ggacagggcc ccctgggagc cgtcgctctt gaacgccaga
     2581 ccagccgtgg taccaccgcc aaatacggca ccccgcagac ttttcaggcg tcgcatccta
     2641 cccatctgac gacctcgctg gcgggacgcc tgctcaccgg cctgtccgtt tcaggactcg
     2701 atcggccggg aagcagcatg gcgcaggaga gttatccgct gttctctcac gaggaagtgg
     2761 agtttctggc cccgctcatc gtcggcgcac tcgcacaggc ccaacctgat cagcgggttc
     2821 gcttcacgat gcaggatgac ggtctcacga cccaaggcac actctacgtg tacaaaacca
     2881 cgctgcgagt gaatctgtcc cactaccgat cagcatccgg ggatggacag accaaacctg
     2941 cgtcactcat actctccttc acgcccccac aagccctcgt gcgagccgat gtccctcagc
     3001 cctggatgac cgtcgagccg gaacagccgc gtatcgccgt ctcccttgag gcgctgagtc
     3061 agcttcctgc gccatcccct gccgcagccg accacaacac aactgccgag gcagcaccga
     3121 cacaaacctc gcccactgcc gaacagagcc gcttgcagca ggaactccaa tccacgaagg
     3181 atgtggtggt caaacaggcg gaggaactcc agaagctcaa ggcagagttg gaatcggtac
     3241 ggcgccagtt agctgagaaa gacgccgctg cgtccaaggc caagccgaaa gcggcgcccc
     3301 gcaagccgac ggcaacaccc taacggcgaa cggcagcggt cattggctgc cggaaaggag
     3361 gtccggctgc gccaagacct ccttcgcgtc atagacgtca tcagcgccca ccggcttctt
     3421 gcccttgtac atcgtcaccg tcatcgacgt tcgtcgtgag agtgccagca catcggcacg
     3481 ctgggcgagg tggaagaaat cgtagccctc gcgcaaggtc agctccgcgc aacgccgtcg
     3541 caggtaggca tccagttgtt cttgcgaggt aaacgaactg acgcggtatt ccacacgata
     3601 gacattctcc ttgagctgct ccacctcatc gcgatcggcc ccgaccaacg gagtgtattg
     3661 cccggtggcg caggcgcttg tcataagagt tagcagaaaa gctgtggcaa cgcgtcggct
     3721 cttcgccatc caaacctcac ctattcccag acagtctcga acatgtgctg ttgcgttcat
     3781 tgatgtgtag acattcagcg aaatcagttc actgtggcac ttgaccgtca ggcgggctac
     3841 aagtcggtct caccccctga gccctccaag agagtaccgc caatgaagca gaatgaggag
     3901 cagaagacaa acgaagcgtg aagtaatctg gaaggaacgg ctcgggctca accccagcac
     3961 cgcgaagatc gatccaactg ccagggcctg ccccctcctc tggtcgtaaa gttcgcctag
     4021 tggcgatcaa ctcctgccct tatcggcccg ctcttcgatt tctccggcca ggagaatggc
     4081 ttctgcgatc tctcgcatgg atttgcgaag atccatgctc tgccgttgaa tcaatttgaa
     4141 cgcgtcttct tccgacagcc gcttcgaccg catgagatat cccttggccc ggtcgagcaa
     4201 cttgcggacc tgcaaggctt cctgcatttc aaacgatttc tcaagcaacg tggtgtgctc
     4261 aatggaaatc gcggcctgat tggcaatcgc ctgcatcaat ttgacatcct ccgccgtgaa
     4321 ggtatgcggc ttggacgtat acgtattgat cacgccgatg gccttctccc gcaccagcat
     4381 cggcacgcac aggagcgagc agaggccctc cttcgcagcc atgtccg
//
LOCUS       DAORQN010000123         4384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_754_length_4384_cov_8.491337, whole genome shotgun sequence.
ACCESSION   DAORQN010000123 DAORQN010000000
VERSION     DAORQN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_754_length_4384_cov_8.491337"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PK614_07085"
     CDS             <1..478
                     /locus_tag="PK614_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82012.1"
                     /translation="FMTTPLETNYTGESFLHLVNLPFHEAGHLLFMPFGRFMTILGGS
                     LGQILMPLVCLGTFLLKTRDPFGGSVALWWTAENFMDVAPYINDARAMDLLLLGGFTG
                     KEVDAHDWNNLLTMLGWLQYDHGLAKLSYGIGTMLMLLALAWGATLLHRQYRRLDW"
     gene            541..1023
                     /locus_tag="PK614_07090"
     CDS             541..1023
                     /locus_tag="PK614_07090"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRB82013.1"
                     /translation="MRTLASLCRIAAVPILLGVGLMAGQVHADEGASRFVLMLDGEAV
                     KDSASGLVWEREPDYLFDVWDRSVARCPTKTVGGQQGWRAPSIDEIKTLVDPDQQDPS
                     LPAGHPFRNIRSGIYWTATPHPKDDIVAWQQSFFSGQAVTDQKSGMRRLWCVLGDVRK
                     "
     gene            complement(1158..1376)
                     /locus_tag="PK614_07095"
     CDS             complement(1158..1376)
                     /locus_tag="PK614_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82014.1"
                     /translation="MNFLAFKGNESENRKDHRRRMAVAIPDEVAAQIIEEPFFDKTKE
                     EFRTFGGMMINPVCRVSLEKETILATAG"
     gene            2096..3775
                     /locus_tag="PK614_07100"
     CDS             2096..3775
                     /locus_tag="PK614_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB82015.1"
                     /translation="MPGRYLELKPVRSTFWGEVSRLALVIGLEQRVLTLIVSYALAIA
                     LFSLVVPLTVQELVSTFAFSIEPVMIVTLTLIILIGLLFIGFFRVFQTSATEILFQRL
                     YTRIAIAMTEHLPRVREEVFVPSYANYFGEAELLPRAVVVVLVDLINVVISGAAGMVM
                     LVVYHPYFLVYNAFLLGGFALVQFTSGQGGVRATQTVSQHHYDLMTWMQDIAQNRLHF
                     KATQSAPLLLNKTDRLLESYLAARRARSGVLTWRQYISTVIWEAICHSGMIGMGGWLL
                     SIGQITLGQFVAAEVIVGTLLLNLDTVTRRIYAVTYILTSFDELTRVFSLPKDDLREE
                     ASFARLPDPAIHGLRVTCKDAGFAYPNSPPIIEGFNLEVAPGEKVAVISHTSTGKSTL
                     ALLLAGLSRPTTGVVRFNDVDLRDMTMDDINAARALVLDSHLTLFGGTLEENISLGRP
                     SVRFEDFRWALKFAELDEEADRLPQGLETPTLAGGKLFTKSQILRILVARAIVTRPQL
                     LIFDGTLHNMEPDLRQILLRRLCSKEETWSVIFVTNDPSIGGYVDRRVMLG"
     gene            3884..>4384
                     /locus_tag="PK614_07105"
     CDS             3884..>4384
                     /locus_tag="PK614_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRB82016.1"
                     /translation="MFRPIPEAALLRRAPYRLIAAVLLILALVVSVILLFSLEQEKLL
                     LQGVAQGKTVPTDLFPALWQSRRDLTLVALLVFLVSAIGITAVITFLHYDSTIRTLEE
                     VKGLARNILESIPTGVLTLNRSGIITAVNPSAEAVLKRASLDLLGHSYESVFTEGDTI
                     RGALDAA"
BASE COUNT          826 a         1265 c         1256 g         1037 t
ORIGIN      
        1 cttcatgacc acgccgctcg agacgaatta cacgggcgaa tcgtttttgc atttggtcaa
       61 tctaccgttt catgaagcgg gccatctgct cttcatgccc ttcgggcgat tcatgaccat
      121 cctcggcggc agtctgggac agatcctcat gccgctggtg tgcctgggca cctttctcct
      181 caagacccgc gatccgttcg gtggatcagt cgccctgtgg tggacggccg aaaacttcat
      241 ggacgtcgcg ccctacatca acgatgctcg cgcgatggac ctcctgttgc tcggcgggtt
      301 caccggcaag gaagtcgatg cgcacgactg gaacaatctc ctcaccatgc tcggctggtt
      361 gcagtatgat catggtctgg cgaagctgtc gtacggtatc gggacgatgt tgatgctgct
      421 tgcgctggcc tggggcgcga cactcctgca tcgacaatac cgacgactgg actggtagcc
      481 cctctcgact cattacgctc tccttgaaaa gacccgacac atcgggcaca ctgccggact
      541 atgcgtacct tggcgtcgct ctgccgcatc gctgctgttc cgatcttgct tggcgtcggt
      601 ctcatggccg gtcaggtcca tgccgacgag ggcgcgtcgc ggttcgtctt gatgctggac
      661 ggcgaagcgg tgaaagattc ggcgagcggg ctggtgtggg agcgcgaacc ggattacctg
      721 tttgacgtat gggatcgctc ggtcgcccga tgccccacga aaaccgtcgg cgggcagcag
      781 ggctggcgtg ccccgagcat cgacgaaatc aaaacgctgg tcgatcccga ccagcaagat
      841 ccttcgttgc ccgcgggtca tccatttcgc aatatccggt ccggcatcta ctggacggcc
      901 acaccccatc ccaaggatga catcgtggcc tggcagcaaa gtttcttctc cggccaggcc
      961 gtgaccgatc agaaatccgg catgcgccgt ctctggtgtg tgctgggaga cgtgcgcaag
     1021 taacctgtcc gatatcgccg gtgatccctt ccacccacgc gtagcctgat tggccggaat
     1081 gattctccat tcttggctct ttggttctaa ggacagtcat ggtaacgcag cttcgagcaa
     1141 ccgttctact tcgagtgtca gccagcggtc gcgagtattg tttccttttc tagggacact
     1201 ctgcacacag gattgatcat cattccaccg aaggttcgga attcctcttt cgttttgtcg
     1261 aagaagggct cttcgataat ctgcgctgcc acctcgtccg gaattgctac tgccatcctt
     1321 cgtcggtgat cctttcgatt ttctgattcg ttgcccttga atgccaggaa gttcattcgg
     1381 gggcgggacg cctacatgca atttttgcag aactacctcc tgaatgttcg ttgggtcagg
     1441 attgcggcga tgtaggcgag tcggcttata ggtccaaacc ctcatagatc caatagattt
     1501 cctggcctgg acagtaaggc atacgagttg cttaggccag caggggaaaa gtcgcatcct
     1561 tcaaatgaac ggatcggaat aggaatgtga agttatggaa ccgacggatc atccttgtga
     1621 gcggtgtggt cgaatcgacg cgcggctcat cttcgaccag tggagcaaat tgctgctctg
     1681 cgaaaattgc ctcgagtgca tgaggtggca gcagacatgg tctcttctgc gcgaggctga
     1741 gatgcctgag gcacaggctg ggtcatccct gggcatgaat gaactaagga gggaaggaac
     1801 gtgaaggagt cttctgttat ctcgaatgat caacaatccg ccgtgatcat gtattgcgag
     1861 cgctgcgcca ggcgctctgt ccaactatgc ttcgacgacc tcagccggca agagctctgc
     1921 tccgaatgcc tgaatcgttt gaccaagcga agaggattta tcaccaggcc agcgaagcca
     1981 agacagtaag gatccccatg gctcgctcct ctctcctgct cgcggcgggg ctgtccctca
     2041 tcacgccgga cctgtgcggc gcgatccggg acagcgttct ccctgagaga ctctcatgcc
     2101 cggtcgctat ttagagctga agccggttcg cagcaccttc tggggggagg tttcccggct
     2161 ggcgctggtg atcggcctcg aacagcgcgt cctcactctc atcgtctcct acgccttggc
     2221 catcgctctc ttctctctgg tcgttcccct gaccgtgcag gaactcgtca gcacatttgc
     2281 gttttcgatc gagccggtca tgatcgtcac cctcacgttg atcattctga tcggactcct
     2341 cttcatcggt ttcttccggg tctttcagac gagcgcgacg gaaatactct ttcagcggct
     2401 ctacacccgt attgcgatcg ccatgacgga gcacctgcca cgggtacggg aagaagtgtt
     2461 cgtgcccagc tacgcgaact actttggaga agcggaatta ttgccgcgcg ccgtggtggt
     2521 cgtgctggtg gacctgatca atgttgtcat ttccggtgcc gccgggatgg tgatgctcgt
     2581 ggtctatcat ccgtatttcc ttgtgtacaa cgccttcctg ctcggtggat ttgcgctggt
     2641 gcaattcacc tccgggcaag gaggggttcg agcgacgcag acggtctcac aacaccatta
     2701 cgatctcatg acctggatgc aggatatcgc gcagaatcgc cttcatttca aggccaccca
     2761 gagcgcccct ttactgctga ataaaaccga ccgcttgttg gagtcttacc ttgcggcgcg
     2821 aagggctcgc tcaggggtgc tgacctggcg ccagtacatc agcacggtga tctgggaggc
     2881 gatttgccat agcggcatga tcggcatggg cggatggctg ctgtcgatcg ggcagatcac
     2941 gctcggtcag ttcgtcgcgg cagaagtcat cgtcggcacg ctccttctga acctcgatac
     3001 ggtgacgcgt cgaatctatg ccgtgaccta tatcttgacg tctttcgatg agctaacgcg
     3061 ggttttctct cttccgaaag atgatctgag agaggaggcg tcattcgcac ggttgcccga
     3121 tcccgccatc cacggactgc gtgtgacgtg caaagacgcg ggctttgcct atcccaactc
     3181 cccaccgatc attgaggggt tcaacttgga ggtggctccc ggggaaaagg tggcggtcat
     3241 ttctcacacc agcacgggca aatccaccct cgccctcttg ctggccggtc tctctcgccc
     3301 gaccaccggg gtggttcgat tcaacgatgt ggatctgcgc gatatgacca tggacgatat
     3361 caatgccgcc cgcgcgctgg ttctcgattc gcatctgacg ttgttcggcg ggacactgga
     3421 ggaaaacatc tcactcggac gtccgtccgt ccggttcgaa gactttcgct gggcgctgaa
     3481 gttcgcggaa ttagatgagg aagccgatag attgccacag ggcctggaaa ccccgactct
     3541 ggcaggaggg aagcttttta cgaagagtca gattcttcgc attctggtcg ctcgcgcgat
     3601 cgtcacgagg ccgcaattgc tcatcttcga tgggacgctc cataacatgg agccggatct
     3661 gaggcagatt ctgctgcggc gactctgttc aaaagaggaa acgtggtctg tgattttcgt
     3721 caccaatgat ccttccatcg gaggatatgt cgatcgccgc gtcatgctgg ggtgagttca
     3781 gccgcgcggt ggtcgccccc ggttcacaga ccgttcggct atacagcccc aaccctgcgt
     3841 ggtagcatga aatcctgctc gggtcttcct ataggacgca ggtatgttta gacctattcc
     3901 tgaagccgcg ctcctccgaa gggctcccta tcggttgatc gcggcggtgc tgttgattct
     3961 ggccttggtc gtttccgtca tcctgctctt cagtctggaa caggagaaac ttctcctgca
     4021 gggcgtcgct caaggcaaga cggttccgac ggatttgttt ccagcgctct ggcagtcgcg
     4081 ccgcgacttg acgcttgtgg cactcctcgt ttttctggtg agtgcgatcg gaatcaccgc
     4141 ggtcatcacg tttctgcact atgacagcac catacgaact ctcgaagaag tgaaggggct
     4201 ggcgcgtaac atcctcgaga gcattcccac cggtgtcctg acgctgaacc gcagcggcat
     4261 cattacggcc gtcaatccct cggctgaggc cgtgttgaaa cgtgcgtcgc tcgacttgct
     4321 gggccactcg tacgaatccg tctttacgga gggcgatacc attcgcgggg cgcttgatgc
     4381 agcg
//
LOCUS       DAORQN010000124         4289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_762_length_4289_cov_6.725319, whole genome shotgun sequence.
ACCESSION   DAORQN010000124 DAORQN010000000
VERSION     DAORQN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_762_length_4289_cov_6.725319"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PK614_07110"
     CDS             <1..923
                     /locus_tag="PK614_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82017.1"
                     /translation="GPPLPMENGARGGPGAARCAAACLFGPSPSDAGEPHQAPCADWQ
                     SRHPEWIWCDDFELDRLSHYFEYDIRHGRFVREAGAGVDRSTGMRAEYLPGDPHGGSL
                     HLAFGKTPSDYMKPVDAGTAKYRDIYWRFDLRLQPGWTGGGADKLTRATILSSDRFAQ
                     AAIGHLWSGALPDSDPERLYLDPASGTDEFGVLRTTTYNDFPRLRWLGAAGGQTSLFS
                     PPHIGKWFCIEVHMKLNNGDADDGVLEYWIDDRLDAQRTGINWIGTYRDYGINAVYLE
                     QYWTSVPFAQIQQRYFDNFVVSTARIGCAR"
     gene            1026..3164
                     /gene="ppk1"
                     /locus_tag="PK614_07115"
     CDS             1026..3164
                     /gene="ppk1"
                     /locus_tag="PK614_07115"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRB82018.1"
                     /translation="MSSPQTPSETQPHASTPKARAHRPATSDLSRPEWYLNRELSLLE
                     FNRRVLDLAKDQKVPLLERLKFLCIVSSNLDEFFEVRVAGLKEQVTHGVEQRGADGLT
                     PSELLARIAALTHQLVHEQYVVLNQSLIPQLADQRIRFLKRADWMPSHIRWMRRFFSR
                     ELLPLLSPVGLDPAHPFPKLLNKSLNFLVTLEGTDAFGRPNGTAIVQVPRSLPRVIHL
                     PVRNAAWPHDFAFLSSVIHAFVHQLFPGMHVTGCYQFRVTRNSNLFVDEEDVDDLRRA
                     LEGQLPERRFGDEVRLEVADNCPPDLVYFLREQFHLDARDVYQCHGPVNLHRLMAVPD
                     LVDRPDLKFQPFTPGIPTTPVPSEDWFDAIRQGDILLHHPYQSFAPVTEFLRQAATDP
                     HVLTIKQTLYRTGADSAIVQSLVDAARGGKEVTVVIELRARFDEEANIELAHDLEEAG
                     AHVVYGVVGHKTHAKMSLVLRREGRMLRSYTHLGTGNYHARNARLYTDFGLLTCDAAI
                     GRDVRTVFQQLTSLGRPGRLKHLLQSPFTLHATLLKWIGRETDRAKQGRPAHIIAKMN
                     ALLEPQIIRALYKASQAGVKIDLIVRGPCALRPGIAGLSEHIRVRSIIGRFLEHSRIF
                     YFLNDGDDRVFLSSADWMDRNFFRRIEVAFPVLDKTLRQRVIDEGLRPYLEDNTHAWI
                     LHRDGTYRRQTPGRRAARSSQQWLMNKLVT"
     gene            3278..3784
                     /locus_tag="PK614_07120"
     CDS             3278..3784
                     /locus_tag="PK614_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82019.1"
                     /translation="MSVAQGIRLHPHHQAFASLRTHLLRSRRMLLMQGVSRPVPQVEQ
                     GLPTDVLDLAACEQERLIDDLITQRTSVTLRHIERALSRMLDTSYGICHRCRADSPLP
                     RLRAQPDATLCFTYKQLSEERASVRRAAWRPDTSAEAVYRSWKPATDWELSCVHLSAP
                     LSRAVPLF"
BASE COUNT          861 a         1415 c         1202 g          811 t
ORIGIN      
        1 gggggccccc cctccccatg gaaaacgggg cgcgaggggg tcccggggcg gcgcgctgcg
       61 ccgccgcctg cctcttcggg ccaagcccta gcgacgccgg tgagccgcac caggccccgt
      121 gcgcagattg gcagagtcgc catcccgaat ggatctggtg cgatgatttc gagctcgacc
      181 ggttaagcca ctacttcgag tacgacatcc gacacggtcg gttcgtccgc gaagccgggg
      241 caggggtcga ccgctcaacc ggcatgcgag cagaatacct gcccggcgat ccccacgggg
      301 gttccctcca cctggcgttc ggcaaaaccc caagcgacta tatgaaaccg gtcgatgccg
      361 ggaccgccaa gtatcgcgac atctactggc ggttcgatct tcggctccag cccggctgga
      421 ccggcggtgg cgccgacaaa ctcaccagag ccacgattct ctccagcgac cggtttgccc
      481 aagccgctat cggccacctg tggagcggag cgttgcccga ttcagatcca gagcggcttt
      541 atctcgatcc ggcgtccgga acagacgaat tcggcgtgtt gcgcacgacc acctacaatg
      601 atttcccgcg cctgcgttgg ctcggcgcgg caggcgggca gacttcccta ttcagcccgc
      661 cccacatcgg gaagtggttt tgcatcgaag tccacatgaa gctgaacaac ggcgatgccg
      721 acgacggcgt gcttgagtat tggatcgacg atcgtctcga cgcgcaacgt accggaatca
      781 attggatcgg cacctatcgc gactacggca ttaacgcggt gtacctggag cagtattgga
      841 cctcggtccc gtttgctcag attcaacagc gatacttcga caacttcgtg gtctccaccg
      901 ctcgcatcgg atgcgcccgg tgacaccggc tgccacctgg atagtccggg cagcatggtg
      961 aaaaatttaa ccgtttcttg atccgccgtt tcgcacggct gagatacact gccgacccac
     1021 tggttatgtc atcgccgcag acaccttcag aaactcagcc ccacgcatcg acgccgaagg
     1081 cgagggctca ccggccggcc acgagtgacc tctcccggcc ggaatggtac ctgaaccgcg
     1141 agctcagcct cctggaattc aaccggcgcg tcctcgatct ggcgaaagat cagaaggttc
     1201 cactgctgga acgattgaag tttctctgca tcgtgagctc caatctcgac gagttcttcg
     1261 aagtccgcgt ggcgggactc aaggaacagg tgactcatgg agtcgaacaa cggggtgccg
     1321 acggactgac cccgtcggaa ctcctggccc gcatcgccgc gctgacgcac cagctggttc
     1381 atgagcaata cgtcgtcctg aatcaatcac tcatccctca gctggccgac caacgtatcc
     1441 gttttctcaa acgcgccgat tggatgccgt cgcacattcg gtggatgcgc cggttttttt
     1501 cgcgcgaact gctcccgctc ctgagtccgg tgggcctgga tcccgcacat ccttttccca
     1561 aactgctcaa caagagtctc aactttctgg tgacgctcga agggaccgat gccttcggcc
     1621 gccccaacgg aaccgcgatc gtgcaggtcc cgcggtcgct cccgcgcgtg attcacctgc
     1681 cggtgcgcaa cgccgcctgg ccccacgact ttgcattttt gtcgtcggtg atccatgcct
     1741 ttgtccatca actcttcccc ggcatgcacg tcaccggttg ttaccaattc cgtgtcacac
     1801 gaaacagcaa tcttttcgtg gacgaggaag acgtggacga cctccggcgg gcgctcgaag
     1861 gtcagcttcc cgaacggcga ttcggcgacg aagtgcggct tgaagtggcg gacaactgcc
     1921 cgcccgacct agtctatttt ctgcgggaac agtttcatct ggatgcgcgc gacgtgtatc
     1981 agtgccacgg accggtcaat ctgcatcgac tgatggccgt gcccgacctg gtggatcggc
     2041 ctgatctaaa atttcagccc ttcacccccg gcattcccac gacgcctgta ccgagcgaag
     2101 actggttcga cgccatcagg caaggcgaca tcctactgca tcatccctat cagtcctttg
     2161 cgccagtgac cgagttcctc cgccaggcgg ccacggaccc gcacgtcctc accatcaagc
     2221 aaaccctgta tcgcacaggg gcggattcag ccatcgtgca atccctggtg gatgcggcgc
     2281 gaggcggaaa agaagtgacc gtggtgatcg aactacgggc ccgtttcgac gaagaggcga
     2341 acatcgagct ggcgcatgat ctggaagagg ccggcgccca tgtggtgtac ggcgtggtcg
     2401 gacacaaaac ccacgccaaa atgagtctcg tgctccgacg ggaaggccgg atgctgcgga
     2461 gctataccca cctcggaacc ggcaactacc acgcccgcaa tgcgcggctc tacaccgatt
     2521 ttgggttgct tacgtgcgat gcggcgatcg ggcgggacgt ccggacggta tttcagcaac
     2581 tgacctccct tggacggccg gggcggctca aacatcttct tcaatcgccc tttaccctgc
     2641 atgccacgct gttgaaatgg atcgggcgcg aaaccgacag ggccaagcag ggccggccgg
     2701 cccacatcat cgcgaagatg aatgcgctcc tggagcctca aatcattcgg gccctctaca
     2761 aggcctctca ggccggggtg aaaatcgact tgatcgtccg aggtccctgc gccttacgac
     2821 ccggcatcgc gggcctctcg gaacatatcc gcgtacgttc gatcatcgga cggtttctcg
     2881 aacatagccg gatcttctat tttctgaacg acggcgacga ccgggtcttt ctctccagtg
     2941 ccgattggat ggatcgcaat ttctttcgac gcatcgaagt ggcgtttcct gtactggata
     3001 aaacattgcg tcagcgcgtg atcgacgagg gcctccgccc ctacctcgaa gacaatacgc
     3061 acgcctggat tctccatcgg gatggcacct accggcgtca aacaccagga cgcagagccg
     3121 ctcgttcgtc ccagcaatgg ctcatgaaca agctcgtcac ctaacctcct ggtaggcgca
     3181 accactcgtc attattaacc cctccttaac cggttgctta tccggttgtc acgtcagccc
     3241 tctatgctgc gggctcgaca acgaggaggg gagtaccatg agcgtcgcgc agggcattcg
     3301 actccatcca caccaccaag cattcgcatc gttacgcaca cacctgcttc gaagccgccg
     3361 catgctctta atgcagggcg tgtcccggcc tgtgccccaa gtagaacaag gtctccccac
     3421 cgatgttctc gatctggccg cttgcgaaca ggaacggctg atcgacgacc tcatcacaca
     3481 acggacttct gtcacactca ggcacattga gcgcgccctc agccgcatgc tcgacacctc
     3541 gtacggcatc tgccatcgct gccgagcgga cagccctctg ccgcgactac gcgcacaacc
     3601 tgatgcgacg ctctgtttca catacaagca actgtctgaa gaacgggctt cggtacgacg
     3661 cgcggcatgg aggcccgaca cctccgcaga agcggtttac cgatcctgga agccggctac
     3721 cgattgggag ttgagctgcg tccacctctc cgcacctctc tcccgggctg ttcccctctt
     3781 ctgagccgga gcggaacagc ccaccatttt gacccgaaac cgcgctcata ggaggccgat
     3841 cccccggccc cctacagccc agcccgatca caccatcgtt gtgagggcga aacagtcagg
     3901 aaaagaaaga ttggaatgac ccgggagcgg tgattggcca cgcgagattc cgtggcgatt
     3961 gccgcatgca atccgcaacc tccagtgtcc gcggcacaat cggcacgaac cagctcagaa
     4021 gcctcggcat ggaggcctga cgcttcctac ccccggcacg atgcctctag caggctacct
     4081 gacggcacgc aacgatccag cgtataccgg aacaggtaca agccgatatt cagacgagcg
     4141 gttggttgcc ctcgcgacta gcccggacta ttcatgcatg ccgtcgcgag gcgttcgaga
     4201 cggaagcccc gcggggcttc tcgcacgtga ggccgacgca ggcgtacttg cagtccgtcg
     4261 aggaggccga acgagaacag ccccgccgg
//
LOCUS       DAORQN010000125         4284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_767_length_4284_cov_5.842516, whole genome shotgun sequence.
ACCESSION   DAORQN010000125 DAORQN010000000
VERSION     DAORQN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_4284_cov_5.842516"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PK614_07125"
     CDS             <1..806
                     /locus_tag="PK614_07125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB82020.1"
                     /translation="VIVVDEFRRGVVWARNEGIARSSGDVIAFADDDVIPPTDWLERL
                     VGALDRCDAAAAGGTFQETDPLLDAIRRRTPFPEREQMDTGGLVGNSGNLMIRREWLA
                     RCQQEDGYVFNPCFGGSGEDWELMWRLRKRGARMVYVPSQVQHLRRAVFGQHLRHSFQ
                     RGVGIARLFLVMRCDQSGVVPQDSLLWGTRGRRTDPRWFQALWTKLIGPFDGKGFQSR
                     RQFWVFWLGEKCQAAGFLWELVTGLFSKRLMPRRAVSQSAGSAAIGPKL"
     gene            812..2137
                     /locus_tag="PK614_07130"
     CDS             812..2137
                     /locus_tag="PK614_07130"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRB82021.1"
                     /translation="MQLRTKAWFGDEDLTIDFPASWKLVEIGPRDEPALTVEAMRECL
                     SRPIGTRRLSEIARGKRNAVIMVDDLTRPTPGADLLPLLVEELTSGGIPAHDITVMLA
                     GGTHPPASADEKAKKVGSGLSSAVKVLAHDSRGDLVNCGRSPAGLPLHINRRVMESEV
                     KIGVGCIYPHPVAGFSGGAKIILPAVCGVETTRMMHDYLRGSRERGGSIQTELRQDMV
                     AVARRVGLDGIVNVTLNRHRAICGLFAGDVVQAHEAGVRAAQQLYAVPMCPEAEVVVA
                     DMYPFDTSWQFAQDRGMWPVEQATGEVSRVVIAACPLGMGTHELFPVDSPLWSRIARR
                     LSHFRLADLDRPLEKLRTVAQLIRRKQHHLMVLSPGLQGQDLTSMFPHAQRFGDWPTL
                     RAALLARHGEGPVTVAVYRCAPFLIPTAAVLGQEVGVSEPTLTRQPTSA"
     gene            2525..4159
                     /locus_tag="PK614_07135"
     CDS             2525..4159
                     /locus_tag="PK614_07135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB82022.1"
                     /translation="MPSASHIPLPPDDGHDARPNEFDSVAAGASPAAGSMVERLDSAS
                     SVVDGVVAGFLFLFVFGSGLVHIESFPPLWFDEGWTVCVARTWVELGHYGCLLRGEPA
                     PPSLAAHFPVVASVAASFTLFGVGVWQTRLVGLLYTLGAFLLLYALARRLYGRSVAIA
                     ALALLLLVPLKWSIHPLYIGRQVLGEMPLLCFLLAGYVCFLRSTHRPFWQVAAIGCWA
                     LAWMTKAQVAPFLVAAMAGTMVVMSLRGDWSVVGRLAVAMIGSWGGCRLLLYAKDWLL
                     AGHIMPHPPVDGMTEAIALVFVPSIRLETIRYLFVSWPEYPLGLAYAAWRVGGTSGSV
                     AKVSVEQTVQTMLLLLAGSWLAWFAFLSAGEPRYALPGLFLAAPFTAALFHDFTRGFD
                     VAFLARTLTGLLRSRHVTRQGIKAVVAVMLLVMMGWVAVQERYAFRAREDDRDLFAVT
                     HYLNTATPPTALIETYDSELFLFLHRPYTYAPPRALVEIIRREQGEAKAVTYDPLESR
                     PEYGRWAGFYKPLIAQNRVRLVTSIGRYQIYEPVRR"
BASE COUNT          692 a         1207 c         1449 g          936 t
ORIGIN      
        1 ttgttatcgt cgtcgatgaa ttccggcgcg gcgtggtatg ggctagaaat gagggtattg
       61 ccagatcttc aggtgacgtg atcgcgtttg cggatgacga tgtgattccg ccgacggatt
      121 ggctggaacg gctggtcggg gccctcgatc gctgcgatgc cgcggcggcg gggggcacgt
      181 tccaggagac cgatccgttg ctggacgcca tccgtcggcg cactcccttt cccgaacggg
      241 aacagatgga taccggagga ttggtcggca acagcgggaa tctgatgatt cgacgggagt
      301 ggctggcgcg atgtcagcag gaggatgggt acgtgttcaa cccctgcttc ggaggttccg
      361 gggaggactg ggaattgatg tggagactgc ggaaacgagg ggccaggatg gtctacgtgc
      421 ccagccaggt acagcatctg cggcgagcgg tgttcgggca gcatctgcgc cattcgtttc
      481 aacgaggagt tggtatcgcg aggctttttc ttgtgatgcg ttgcgatcag agcggggtcg
      541 tgccgcaaga tagtctgttg tgggggacga gagggcggag gacggatccc cgctggttcc
      601 aggccctgtg gaccaagctc atcggcccat ttgacgggaa ggggtttcag tccaggcggc
      661 agttctgggt gttttggctt ggagaaaaat gtcaggcggc cggatttctt tgggagctgg
      721 tgacgggcct gttctcgaag cggcttatgc ctcgccgagc ggtttcgcaa agcgccgggt
      781 ctgcggcgat agggcccaag ctatgacccg gatgcagttg cgcacgaaag catggttcgg
      841 ggatgaagac ttgacgatcg actttcccgc gtcgtggaag ctggtcgaaa tcggcccccg
      901 ggatgaaccg gcgctgacgg tggaggccat gcgggagtgc ctctctcggc cgatcgggac
      961 gcgcaggttg tctgagattg ccaggggcaa gcgcaatgcc gtgatcatgg tcgacgatct
     1021 gacgaggccg acgccgggag ccgatctgct tccgctcctc gtggaggagt tgacgagcgg
     1081 ggggatccct gcgcacgaca ttacggtgat gttggcgggc ggtacgcacc cgcctgcctc
     1141 ggccgacgag aaggccaaga aggtcggatc cgggctgtca tcggcggtga aggtcctggc
     1201 ccatgacagt cggggcgatc tggtgaattg tggccggtcg cctgccgggc tcccgctcca
     1261 tatcaatcga cgggtgatgg agagcgaggt caagatcggc gtgggctgca tctatcccca
     1321 tccggtggcg gggttttccg gtggcgccaa gatcatactc ccggccgtct gtggcgtgga
     1381 gaccacgcgg atgatgcacg actatctgcg cggttcgcgc gagcgcggcg ggtccatcca
     1441 gacggagttg cggcaggata tggtcgcagt ggcacgacgg gtcgggctcg atggtattgt
     1501 caatgtcacc ctcaatcgcc atcgcgccat ctgcggtctg ttcgcggggg atgtcgttca
     1561 ggcgcatgaa gcaggcgtcc gcgcagcgca acagctctac gccgtgccga tgtgtccgga
     1621 agcggaggtc gtggtggcgg acatgtatcc cttcgacaca agctggcagt ttgcgcagga
     1681 tcgaggaatg tggccggttg agcaggcgac aggagaagtc tcccgggtgg tcattgccgc
     1741 ctgtcctctc ggcatgggca cccatgaatt gtttcccgta gacagtccgc tctggtcgcg
     1801 cattgccaga cggctcagcc attttcgcct ggccgatctc gatcggcccc ttgagaaact
     1861 gaggacggtg gcccaattaa tccgccggaa gcagcatcac ctcatggtgt tgtcgccagg
     1921 cttgcagggg caggacctaa cgtccatgtt cccccatgcg cagcgattcg gagattggcc
     1981 gactctcagg gccgcccttc tggcgcgcca tggcgagggg cctgtgaccg tggcggtcta
     2041 tcgatgcgcg ccgtttctca ttccaaccgc agccgtactg gggcaggagg ttggagtgtc
     2101 ggagccgacc ctcaccaggc aaccgacctc ggcctgaaaa ggaaggagcg gaggatgtcg
     2161 tcctccgcga tgcttcgacg accggtccgt ttctggcagg atgcggaaaa agtccgccag
     2221 cggcgatctc gcaagacacg gccgcctcac cgtctcggcg gcgttcacaa acgtgacgcg
     2281 cgttattccg cgcgtcgtga acctcagagg ctcaccgtac ggccacggga ccccgagcct
     2341 gtctttgcag gcgcggggtg ggcgggtacg aatgaacgat tcgcctcgtc gctacttgcg
     2401 gccttgctgg acggactttt ttcgcatcct gcgggtcatt gtcataccga ccccgcgcat
     2461 gagttgatcg tcgcatattg cgcaatgatc gagtttttcg cagcctgcta gaacttcatc
     2521 cgctatgcct agcgcctctc acattccgtt accgcctgac gacggtcatg atgcacgccc
     2581 taacgagttc gactccgtgg cggccggtgc ctcacctgcg gccggttcca tggtcgaacg
     2641 gctcgactcc gcttcatcgg ttgtggacgg agtggttgcc ggattcttat tcttgttcgt
     2701 gttcggcagc ggcctggtac atatcgagag tttccctccg ctgtggttcg acgaaggctg
     2761 gacagtctgt gtcgcgcgca cctgggttga gctagggcat tacgggtgcc tgctacgcgg
     2821 tgagccggcg ccgccctctt tggcggcgca tttcccggtg gtggcctcgg tggcggcgag
     2881 tttcacactg ttcggcgtcg gtgtctggca gacgagactg gtgggattgc tctataccct
     2941 cggcgccttc ctgctcctct atgcgttggc ccgtcgcctc tatggccggt cagtcgcaat
     3001 tgctgccttg gcgctgctgc tccttgtgcc tctgaagtgg tcgatccatc ccctgtatat
     3061 tggtcgacaa gtgctgggcg agatgccgct cttgtgtttc ctcctggccg ggtatgtctg
     3121 ctttctcagg agcacacacc ggccgttctg gcaggtcgct gcgattggat gctgggcgct
     3181 ggcgtggatg accaaggcgc aggtcgcgcc gtttctcgtc gccgccatgg ccgggaccat
     3241 ggtggtcatg agccttcgcg gggactggtc ggttgtcggc cggctggccg tcgccatgat
     3301 cggttcatgg ggtgggtgcc ggcttcttct gtatgccaag gattggctgt tggcaggcca
     3361 catcatgccg catccgcccg tagacggaat gacagaagcg attgcgttgg tgtttgtgcc
     3421 gtccattcgt cttgagacga tccgatacct gttcgtgtcg tggccggagt atccgctcgg
     3481 gcttgcctat gcggcctggc gtgtaggtgg aacttccggt tccgttgcca aggtttctgt
     3541 tgaacagacg gtgcagacca tgctgctgct gctggcaggg agttggttgg cctggtttgc
     3601 gtttctcagc gccggtgaac cgcgctatgc cctgcccggg ttgtttcttg cggcgccctt
     3661 cacggcagcg ctctttcacg atttcaccag aggattcgat gtggcgttcc tggcgaggac
     3721 tctgaccggc ctcctgcgaa gcaggcatgt gacgaggcag ggaatcaagg cggtcgtcgc
     3781 cgtcatgctc ctggtgatga tggggtgggt tgccgtgcag gagcgctatg cgtttcgcgc
     3841 gcgtgaggac gatcgtgacc tgtttgcggt gacgcactac ctgaatacgg ctacgccgcc
     3901 gacggcattg atcgagacct atgacagcga actgtttcta ttcctgcatc gaccctatac
     3961 ctatgccccg ccccgcgccc tggttgagat tatccggcgc gaacagggag aggcgaaggc
     4021 cgtcacgtac gatccgttgg aaagcaggcc ggagtacggc cgatgggccg gattttacaa
     4081 gccgttgatc gcgcaaaacc gggtgcgact cgtaaccagc atcgggcgct accagatcta
     4141 cgagcccgtg cgccggtaac tatcacgccg caaaaattcc ctccccgcgc gcgtgcatag
     4201 gttcatgtct atttggtacg tgatgccagg cgggtggcaa tgaccatctg acgaatggtc
     4261 tcgctgatga gcgtcggctg atcg
//
LOCUS       DAORQN010000126         4179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_782_length_4179_cov_25.899855, whole genome shotgun sequence.
ACCESSION   DAORQN010000126 DAORQN010000000
VERSION     DAORQN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_4179_cov_25.899855"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PK614_07140"
     CDS             <1..972
                     /locus_tag="PK614_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRB82023.1"
                     /translation="ATVLVQSGTLHVGDAFVVGNFSGRVRALVTDTGSKTTEAGPSVP
                     VEVIGLPGVPSAGDLFTIVKDERVAREIAQERAMKQRAADLAGPAKVSLDDLFAKIQE
                     GNVKELPIVIKADVQGSAEALAAAVEKMPAGAVKLRVMHTGVGGITETDILLAAASKA
                     IVIGFNIRPEPKAAALAEREGVDVRLYSIIYDALNDIRAAMEGLLEPTLKERVLGRAE
                     VRQMFTIPKAGLVAGCYVVDGVISRASVGVRVIRDSVVAYEGKLGSLRRFKDDVREVQ
                     QGYECGITVENFNDLKAGDIIEAYVVDKIAAKLEPANRGTSPQSHRA"
     gene            969..1250
                     /locus_tag="PK614_07145"
     CDS             969..1250
                     /locus_tag="PK614_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRB82024.1"
                     /translation="MTVGLCTVELFIPDGHSLKEKRQVLQSLKTRLRDKFNLSVAEVG
                     DQDLWQKAILGMACVANESAHVNQVLDQAVNLIQSVPVIQLVRSRIELL"
     gene            1260..1658
                     /gene="rbfA"
                     /locus_tag="PK614_07150"
     CDS             1260..1658
                     /gene="rbfA"
                     /locus_tag="PK614_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRB82025.1"
                     /translation="MTKSTYSRADRVADQIRMEVADILMRKIKDPRVRSVTVTDVELT
                     KDLRIARVFVTTMEQNKDERLVFDGLAKASGFVRAELGRRLALRYLPELIFMKDISGP
                     RADRVLELLDGLHRDQSHEEMLAETPHTDV"
     gene            1668..2693
                     /gene="truB"
                     /locus_tag="PK614_07155"
     CDS             1668..2693
                     /gene="truB"
                     /locus_tag="PK614_07155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRB82026.1"
                     /translation="MIASMEPRATTVLQDGVLNVRKEAGWTSHDVVARIRGKLRGMKL
                     GHAGTLDPAATGVLPLLVGRGTRIAEYLLEWDKTYLAGLRLGETTDTQDATGTVLLRS
                     PIESLSATRIREVVAGFEGRIQQLPPMYSAVKVGGVPLYKAARAGREVSRQAREVTVF
                     CLDIVDVQIPEVTLRVVCSKGTYIRTLCADIGDKLGVGGHMATLVRERVGPLTLEQAL
                     TVDEVESRLEQGTLAASMLTLDQALLGLPVCIVGAATAGRVLHGMPVPASEVLAWHGL
                     SSPLSEGANRAIRIKDGVGRLLAIGTMPDGMNAEAPGANAQSIAVSKVLVTEESQGGS
                     IANSIEE"
     gene            2700..2969
                     /gene="rpsO"
                     /locus_tag="PK614_07160"
     CDS             2700..2969
                     /gene="rpsO"
                     /locus_tag="PK614_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRB82027.1"
                     /translation="MALVKEVKTELVKGFQQHDKDTGSPEVQIAILTNRITYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRKVEEARYRAILERLGIRK"
     gene            3166..>4179
                     /locus_tag="PK614_07165"
     CDS             3166..>4179
                     /locus_tag="PK614_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRB82028.1"
                     /translation="MKHVVDIELAGRRLTLETGRIAKQADGAIWATYGDTVVLATAVA
                     SQNAKPGVDFLPLTVDYQERTYAAGKIPGGYFKREGRPSEREVLTSRLIDRPLRPLFP
                     EGYYFETQVIASVLSADKTGVSDVIGIIAASAALAVSSIPFNGPIAGVKIGRVNGQLV
                     VNPDLETLETSDLHLVVAGTADAVMMVEAGANELPEATMLEAIELAHSEIKKIVAKIE
                     ELRKLAGKPKRTVVQEPIDAALTEQVRSLVAGPIREAILIPNKSARQERLDQVLAETV
                     AKLKSDEPNRDRHVKIIFHGLEYTEVRNMILEKRVRADGRGPADIRPITCEVGVLPRA
                     HGSA"
BASE COUNT          854 a         1057 c         1406 g          862 t
ORIGIN      
        1 gcgacggtgc tggtgcagag cgggacgctc catgtcgggg atgcatttgt agtcggcaat
       61 ttcagcggcc gtgtgcgcgc gctcgtgact gacacgggga gcaagacgac tgaggctggc
      121 ccatctgttc ctgtcgaggt gatcggtttg ccgggcgtgc cgtcggccgg cgatttgttc
      181 accatcgtga aagatgaacg ggttgcccgt gagattgcgc aggagcgggc gatgaagcag
      241 cgggcggcgg atctggctgg gccggcgaag gtgagcctgg atgatctgtt tgccaagatt
      301 caggaaggca atgtcaaaga attgcccatc gtcatcaagg ccgatgtgca ggggtcggcc
      361 gaggccttgg ctgccgcggt ggaaaaaatg ccggccggcg cagtcaagtt gcgtgtaatg
      421 cataccgggg tgggcggtat taccgaaacc gacattctct tggcggccgc gtccaaggcc
      481 atcgtgatcg gatttaatat ccgtccggag ccgaaggcgg cggcgttggc cgagcgggag
      541 ggcgtcgatg tgcgcctgta cagcattatt tacgatgcac tcaacgatat ccgcgccgcg
      601 atggagggct tactcgaacc cacgctcaag gagcgtgtcc ttgggcgggc tgaagtacgg
      661 cagatgttta cgatccccaa ggccggtctg gtggccggct gttacgtggt ggatggcgtc
      721 atctcccggg ccagcgtcgg cgtgcgcgtg attcgggata gtgtggtggc ctatgaaggc
      781 aagctgggct cgctccgtcg cttcaaggat gatgtgcgtg aagtgcagca ggggtatgag
      841 tgcggtatca cggtcgaaaa cttcaatgat ctcaaggccg gggacattat cgaggcctac
      901 gtcgttgata agattgccgc caagcttgag cctgcgaacc gtggcacgtc tccgcaaagt
      961 catcgggcat gaccgtcggg ctctgcacgg ttgagttgtt cattcccgac gggcattcgt
     1021 tgaaagagaa acgccaggtc ctgcaaagcc tgaagactcg gttgcgagac aagtttaatc
     1081 tatcggtcgc ggaagtcgga gaccaggatt tgtggcagaa ggcaatttta ggtatggcct
     1141 gtgtggcgaa tgaatccgcg catgtgaacc aggtgttgga tcaagccgtg aatttgattc
     1201 agtccgtgcc tgtgatccaa ctagtacgct cgcgaatcga gttgctgtaa ggcggcgtca
     1261 tgaccaaatc gacgtacagc cgggccgatc gtgtggcaga ccagatccgc atggaagtgg
     1321 ccgacattct gatgcggaag attaaggatc cgcgtgtgcg ctcggtcacg gtgaccgatg
     1381 tggagttgac taaagatctt cgcatcgcgc gcgtctttgt gacgaccatg gagcagaata
     1441 aggatgagcg gttggtgttc gacgggctgg cgaaggccag cggtttcgtt cgggcggagt
     1501 tgggacggcg tctggcatta cgatacctgc cggagctgat ctttatgaaa gacatcagtg
     1561 gccctagagc tgaccgagtc ctggagctgt tggatggact gcaccgcgat caatcccacg
     1621 aagagatgtt ggcagagacc ccccacaccg atgtctagca aatgaccatg atcgcctcga
     1681 tggagccacg cgcgactact gtgcttcagg acggagtgtt gaatgtccgc aaggaagccg
     1741 ggtggacgtc gcacgatgtg gtggctcgca ttcgtgggaa gttgcgcggc atgaagttgg
     1801 gccatgccgg aacgctggat ccggcggcga ccggcgtcct gccgttgctc gtcgggcgtg
     1861 gaacgcgcat tgctgaatat ttgctggaat gggacaaaac ctatctcgcc ggattgcgcc
     1921 tcggtgaaac aaccgacacg caagatgcca ccgggaccgt gctcctgcgt tcaccgattg
     1981 agtcgttgtc cgcaacccgc atccgtgaag tggtagccgg gtttgagggg cgcattcagc
     2041 aactccctcc catgtattct gcggtaaaag tcggcggggt tcccttatac aaagcggcgc
     2101 gagccgggcg ggaggtctcc cggcaggctc gtgaagtgac cgtcttttgc ctcgatatcg
     2161 tggatgttca gattcctgaa gtgacgctcc gggtggtctg ctccaagggg acgtatatca
     2221 ggacgctctg cgcggatatc ggcgacaagt taggggtcgg cggtcatatg gcgacgttgg
     2281 tccgcgagcg cgtggggcca ctgacccttg agcaggcgtt gacggtggac gaagtcgaat
     2341 cacgcttgga gcaaggcacg ctggcggcgt cgatgttgac gttggatcag gcgctgctcg
     2401 gacttccggt ttgcatcgtg ggggcggcga cggccggccg ggtcctgcac ggcatgccgg
     2461 ttcctgcctc agaggtgctg gcctggcatg gtttgtcttc gcctctctct gagggggcga
     2521 atcgggccat tcgcatcaag gacggagtgg ggcggttatt agccatcggc acgatgccgg
     2581 acggcatgaa tgctgaggcg ccgggggcga atgcgcagtc gattgctgta tcgaaagtat
     2641 tggtcacaga agaatcacag ggtggtagta tcgcgaactc aatagaggag tagagacgta
     2701 tggcactggt aaaagaagtg aaaacggaat tggtcaaggg tttccagcaa catgacaagg
     2761 acaccggatc tccggaagtg cagatcgcga tcctgacgaa ccggattacg tacttgacgg
     2821 aacatttcaa gttgcacaag aaggaccacc attcccggcg cgggttgttg acactggtcg
     2881 gacgtcgtcg tcggttgttg gactatctcc gaaaagttga agaagcccgg tatcgcgcca
     2941 ttctggagcg actgggtatt cgtaagtagt cgtgtgagcg aatcggctgc caccttcgtg
     3001 ggtggcggcc gaaccatttg ttgtccccgc aggtgaacac tgaagcaagt tcaacggcac
     3061 gggtcgacat tcaatcgagg gcaggcgctc gtcgttggat gtgtagatgg ccggttgaac
     3121 ttatctctgt gggcatctgt gggatttaaa cggaggagac catagatgaa acatgttgta
     3181 gacattgagc tggcagggcg tcggttgacg ctggagaccg gtcgtatcgc gaagcaggcg
     3241 gacggcgcca tctgggccac ctacggtgat accgtcgtgc tcgcaacggc cgttgcgtca
     3301 caaaatgcca agccgggagt ggatttcctt ccgctgaccg tggattacca ggaaaggact
     3361 tacgcagccg gtaagattcc gggcggctat tttaagcggg aaggtcggcc gtcggagcgg
     3421 gaagtgctga ccagccggct gatcgatcgg ccgcttcggc ctctctttcc ggaagggtat
     3481 tacttcgaga cgcaagtcat tgcctcggta ttgtctgccg ataagacagg cgtgtcggat
     3541 gtcatcggca tcatcgcggc atccgcggcg ttggcggtct cgtcgattcc attcaacggg
     3601 ccgatcgcag gcgtgaaaat cggccgcgtc aacgggcagt tggtggtgaa ccccgatctg
     3661 gaaaccctgg aaaccagcga tcttcatctc gtcgtggccg ggactgccga tgcggtgatg
     3721 atggtcgaag ccggggccaa tgaattgccg gaagcgacca tgctggaagc cattgagttg
     3781 gcgcacagcg aaatcaagaa aatcgtcgcg aagattgaag aattgcgcaa gctggccggc
     3841 aagccgaaac ggacggtggt gcaagagccc atcgatgccg cattgaccga acaggtgcgt
     3901 tcgctggtgg ccggtccgat ccgcgaggcc atcctcatcc ccaataagag cgcaaggcag
     3961 gagcgcctgg atcaggtgtt ggccgagacg gttgcgaagc tcaaatcgga cgagccgaat
     4021 cgggaccggc atgtcaagat cattttccat ggtctggagt acaccgaagt tcgcaacatg
     4081 attctcgaaa agcgggtgcg cgccgacggg cgtggtccag cggacattcg tccgatcacc
     4141 tgcgaagtcg gtgtgctgcc tcgcgcgcac ggatccgca
//
LOCUS       DAORQN010000127         4118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_797_length_4118_cov_5.315038, whole genome shotgun sequence.
ACCESSION   DAORQN010000127 DAORQN010000000
VERSION     DAORQN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_4118_cov_5.315038"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK614_07170"
     CDS             complement(<1..463)
                     /locus_tag="PK614_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82029.1"
                     /translation="MIRQAAVPLVLFAALLASGCAEPKAASGINGVTPVHLPDVRTPV
                     QLDADARQEHRAVMLQHLETVQAIVAALADEDYRLAQGLTETHLGFFMHRHAMARQQP
                     ENFPPAYHDLAMAHHEAAEKLADVMPSRDLKRILPEFNKLLKACVACHLEYK"
     gene            complement(460..1398)
                     /locus_tag="PK614_07175"
     CDS             complement(460..1398)
                     /locus_tag="PK614_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB82030.1"
                     /translation="MWQRETFKKIKQIPGEEHVLPGSALCAGCGGLESLRLAAKVLGD
                     DVVYVNAAGCFTMLAAYPYTSFKGSWLYTTMGSAPAGAQGVRDALDVLIAKGRLPKSD
                     NLKVVVLGGDGSTYDMALSSTSGAMNRGLDFYYICYDNEAYGNTGMQLSPATPYGART
                     ATSPCSLQHPSGASQEKKDIFEIWRAHKPPYIATVAPRYPLDLEEKFARAAAFTGPKM
                     FLTLAACPTGWLYDPGETPEVAKLAVETGLWPLKEAINGVVTHTYVPKRKPVEAYLKL
                     QGRFRHLFEPTVQTEAIQHIQARVDAYWERATREGS"
     gene            complement(1455..2693)
                     /locus_tag="PK614_07180"
     CDS             complement(1455..2693)
                     /locus_tag="PK614_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HRB82031.1"
                     /translation="MDSQMMTGNLAAAWGARLADVDYIPAFPITPQTEIVEALAKWCE
                     GGELAARLVTMDSEHSMMTAAGAAEATGARVFTATSSQGLLYAFEVLYSISGWRAPLV
                     LVNVSRALAAPITLESDHNDVLAARDSGFLQIHAETCQEVLDSILMAYRIAEDERVML
                     PAIVNLDGFSLSFTREPVSIPDAEQVRKFLPPFRPAHLGFKASAPHSLGVAVIGGTPY
                     AYFRHQMHLASINALEVHREVAASFEQLFGRRYDAVEGYRLDDAEDVLVMTNSCAGTG
                     KAAVDAAREEGKRVGLLRLRMIRPWPVDAICGALRGRRAVAVLDQNLAPGQGGILFQE
                     IAASLYHEVERPRALCSFIGGLGGQNLSVGEFHAIFEQTAKAGVSGKGIGPVLLYTQV
                     EHREMTQLQMVAAGSVKRET"
     gene            complement(2678..3595)
                     /locus_tag="PK614_07185"
     CDS             complement(2678..3595)
                     /locus_tag="PK614_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRB82032.1"
                     /translation="MYAVRFHGRGGQGAKTASRILGTAAFLSGYQAQDSPIYGAERRG
                     APVAAFTRFGREPIRERGVIAHPDLVVVADASLLEAPAARVSDGITDQTILFVNSPLH
                     AEQIRTQSGLPGRITTLGVTEIALQHFGQREAISALLGAVAARLSGLQQEPLRQAIAR
                     ELEDLGVAPPIIERNQTVALTCYESVQVMERESVVAPAAIATTFHTPVYESPSRGTAR
                     ISATGNSVLRETGGWRTFRPVLVADKCNGCWLCFVYCPDGVISMNTDDRPMIDYAHCK
                     GCQICVHECPTHALVAEREQEGGVAWTAK"
     gene            complement(3597..3905)
                     /locus_tag="PK614_07190"
     CDS             complement(3597..3905)
                     /locus_tag="PK614_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRB82033.1"
                     /translation="MADHHDSCSGLGVSIAFLSGAMLGAMAAILYAPSSGEETRRAIK
                     GYARRTEDEVLEKAKEIRADLSHTVDEAKRYLKETEATIAAALAAGKEAFKKEKTDRA
                     "
BASE COUNT          810 a         1375 c         1144 g          789 t
ORIGIN      
        1 gtttgtactc caggtgacag gcaacacagg ctttcagcag tttgttgaac tcgggcagga
       61 tccgcttcag gtcccgcgac ggcatcacat cggccagctt ttccgcggct tcatgatgcg
      121 ccatcgccag gtcatggtag gccggcggaa aattctccgg ctgctggcga gccatcgcgt
      181 gccggtgcat gaaaaacccc aagtgtgttt cggtcaagcc ttgagcgagc ctgtaatcct
      241 cgtcggccag cgctgcaacg atcgcttgaa ccgtctccaa atgttggagc atgacggcgc
      301 gatgttcctg tcgggcgtcg gcatcgagct ggaccggcgt gcgcacatcc ggcaaatgaa
      361 ccggcgtcac accattgatg ccggatgcag ccttcggctc cgcgcagccg ctcgcaagga
      421 gcgccgcgaa gagcaccagt ggaacagcgg cttgtcgaat catgatccct ccctggttgc
      481 ccgctcccaa taggcgtcca cgcgcgcttg aatgtgctga atcgcctccg tctgcacggt
      541 cggttcgaag aggtgccgga aacgcccctg taacttcaga taggcttcga ccggcttgcg
      601 cttcgggacg taggtgtggg tgaccactcc attgatggct tccttcagcg gccagagccc
      661 ggtctcgacc gccagcttcg cgacttccgg cgtttcccct ggatcgtaga gccagccggt
      721 cgggcaggcg gctaacgtga gaaacatctt cggccccgtg aaggcagccg cccgcgcaaa
      781 tttttcttcc agatccagcg ggtatcgcgg agcgacggtc gcgatgtacg gcggtttgtg
      841 cgcgcgccag atttcaaaga tgtccttctt ctcttgagac gccccgctcg gatgctgcag
      901 gctgcaaggc gaggtggctg tccgcgcccc ataaggggtc gccggggaca gctgcatgcc
      961 ggtgttcccg tacgcttcgt tgtcataaca gatgtaatag aaatcgagtc ctcgattcat
     1021 cgcgcccgag gtggacgaca gcgccatatc gtaggttgaa ccgtcgcccc ccagcaccac
     1081 gactttcagg ttgtcgctct tcggtaaccg acccttggcg atcaatacgt ccaacgcatc
     1141 acgtacgccc tgcgcaccgg caggcgcgga gcccatcgtc gtatagagcc atgagccctt
     1201 gaatgacgtg tagggatagg ccgccaacat ggtgaagcaa ccggcagcgt tgacatacac
     1261 gacgtcgtcg ccgagcacct tcgcagcaag tcgcaatgac tccaacccgc cgcaccctgc
     1321 gcagagcgcg cttcccggaa gcacatgctc ctcacctgga atctgtttga tcttcttaaa
     1381 tgtctctcgt tgccacatgg cgtctctcgc ggtttgtatc tctcagcgtt caaacggtca
     1441 gtaggattcc ctcttcacgt ttcacgtttc acgcttcctg ccgcaaccat ctggagctga
     1501 gtcatctccc gatgctcgac ttgagtgtag aggagaaccg gaccgattcc ttttccgctg
     1561 actccggctt tcgccgtttg ctcgaagatc gcgtgaaact ctcccaccga tagattctgt
     1621 cccccgagcc ctccgatgaa ggaacagagt gcccgcggcc gctcgacttc gtgatacaga
     1681 gaggcggcaa tctcctgaaa aagaatgcca ccctgccccg gcgcgagatt ctgatccaac
     1741 acggccacag cgcgacgccc tcgcaatgcg ccacagatgg catcgaccgg ccagggccga
     1801 atcatccgga gccgcaacaa gcctacccgt ttaccctctt ccctggctgc atcgaccgcc
     1861 gccttgccgg tgccggcaca ggaattcgtc atcacgagca catcctccgc atcatccagc
     1921 cgatacccct cgaccgcatc gtatcgccga ccgaacagct gctcgaaaga agcggccact
     1981 tcccgatgca cctccaacgc attgatcgag gccagatgca tttgatgccg gaaataggca
     2041 tagggcgttc cgccgatcac cgccacccct agcgaatggg gcgcagacgc tttgaaaccg
     2101 aggtgggcgg gacggaacgg cggcagaaat ttccgaactt gctccgcatc cgggatgcta
     2161 acaggctcac gcgtgaacga gagcgaaaag ccatccagat tcacgatggc cggcaacatc
     2221 acccgctcat cctctgcgat ccgataggcc atcagaatcg aatccaacac ctcctggcag
     2281 gtctcggcat ggatctgcag aaaaccggaa tcacgcgccg ccaacacatc gttatgatcc
     2341 gactccaacg taatgggcgc agccaacgcc cgtgacacat tgaccagcac gagcggagca
     2401 cgccaaccgg agatcgaata gagcacctcg aaggcataga gcagtccctg gctggatgtt
     2461 gcggtgaaga cccgggctcc cgtggcctct gccgcgcccg ccgccgtcat catcgaatgt
     2521 tccgagtcca tcgtgacgag ccgcgccgcc agttcaccgc cctcacacca tttggccagc
     2581 gcttccacaa tttccgtttg cggtgtgatg ggaaacgcag gaatgtaatc cacgtcagcc
     2641 agtctcgccc cccaggccgc cgccaggttg ccggtcatca tttggctgtc catgccactc
     2701 cgccttcctg ttcccgctcc gcgacgaggg catgcgtggg gcactcgtgc acgcagatct
     2761 gacagccttt gcaatgggca tagtcgatca tcggccggtc atccgtgttc atcgaaatca
     2821 ccccgtcggg gcaatagacg aaacaaagcc agcagccgtt acacttgtcg gcgaccagca
     2881 caggcctaaa cgtccgccac ccgccggttt cacgcagcac ggaattcccg gtggcgctga
     2941 tcctggccgt ccctctgctt ggcgattcat agaccggggt gtggaaagtc gtcgcgatag
     3001 ccgccggcgc taccacagac tcacgctcca tcacctgcac cgactcataa cacgtgagcg
     3061 cgacggtttg attccgctca atgatcggcg gggctactcc gagatcttcc aactcccgcg
     3121 caatggcctg ccggagcggc tcctgttgca ggccggacaa ccgggcggcg acggcaccga
     3181 gcaatgcgct gatcgcctcc cgctggccga aatgctgcag ggcaatctcc gtcaccccca
     3241 acgtcgtgat gcgaccgggc aacccgctct gcgtgcgaat ctgctctgca tgcagcggcg
     3301 aattgacgaa caggatggtc tggtcggtga tgccgtccga cacacgggct gcaggagctt
     3361 ccagcagcga tgcatcggcc accaccacca gatcgggatg ggcaatgacc cctcgttcgc
     3421 ggatcggctc ccggccgaac cgcgtgaatg ccgcgaccgg cgccccgcgg cgctcggctc
     3481 catagatcgg ggaatcctga gcctggtacc cgctcagaaa cgccgccgtg ccgagaatcc
     3541 ggctcgccgt cttggcgccc tgtccccctc gcccgtgaaa tcgcacggca tacatttcag
     3601 gcccgatccg tcttttcttt cttgaacgct tcctttcccg ccgccagagc cgcggcaatc
     3661 gtcgcctcgg tctctttcag ataccgcttg gcctcatcaa cggtgtggga gaggtcagca
     3721 cggatctctt tggccttttc caagacctcg tcctccgtcc gacgcgcata gcctttgatc
     3781 gcccgacggg tttcctcgcc ggaactgggc gcatacagaa tcgccgccat cgcacccagc
     3841 atcgccccgc tcaaaaacgc gatcgacacc ccgagtcccg aacagctgtc atgatggtcc
     3901 gccatgatga tgtctcctcc ctgctctatt gattgctccg gcttcagcct atcccctcga
     3961 tacccctctt gggagaaagc agaggacgtg ccaggcaagg atgccggcgc agcaacgaaa
     4021 tacccgcttt ggcaagggtt ttcagggagc agcgaaccgc aacaacggag ggggatttcg
     4081 attggggaat tccagaaccg gcccggactc gacactgg
//
LOCUS       DAORQN010000128         4032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_811_length_4032_cov_8.366105, whole genome shotgun sequence.
ACCESSION   DAORQN010000128 DAORQN010000000
VERSION     DAORQN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_811_length_4032_cov_8.366105"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="thpR"
                     /locus_tag="PK614_07195"
     CDS             complement(<1..529)
                     /gene="thpR"
                     /locus_tag="PK614_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRB82034.1"
                     /translation="MVRAFLAIELPQELRTTLATIEQDMKRQLERTVDRHVRISWVRP
                     DSMHLTLRFLGDTADESIEPLRVAIEESVSAHQAIPIPLERLGTFPRRQAPRVLWAGP
                     SVSWEQGLEAQRLTSLHRAVEACCRAANFAPEERPFSPHLTLARIKEGERQVGQALAQ
                     NGLLERPVAIGMLPVE"
     gene            complement(529..1809)
                     /locus_tag="PK614_07200"
     CDS             complement(529..1809)
                     /locus_tag="PK614_07200"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRB82035.1"
                     /translation="MSKTKTWTAETIAVGSELLLGGRLDTNSLFIADRLAACGIELRY
                     KTTVGDDLSDIVAVLKTALRRARVVILTGGLGPTIDDLTREAVAQATGHRLSRRKAAL
                     DGMIARLVEWGRTPTTTQFRQAMIPARAEILFNPVGTAPGFSLVWKGTFLAALPGVPR
                     EMEPMVRDGVLPQLQAWMRTQKRLSASPMIRRVLHTSGVPESIVDQALVGLVPKGCTI
                     QLGIYASPMEVMVSLTGPAGAEGAVTIEPLLKEAKTRLGDIVYGEEDETMESVVGRLL
                     NEQQLTLAVAESCTGGLIGHRLTQVPGASTYVDRVAVTYSNRAKVEMLGVPSGLLKRH
                     GAVSADVAAAMARGIRERSGASVGVSVTGIAGPGGATESKPVGLVYVGLDGGPGEAMT
                     KEFRFHGGDRTVIKQRSSQAALDLLRRWLIKKGC"
     gene            complement(1857..2429)
                     /locus_tag="PK614_07205"
     CDS             complement(1857..2429)
                     /locus_tag="PK614_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82036.1"
                     /translation="MGSLFVVLWGSTGSSYAIEVQPTTEQIQAQVERGKLAAGQGVSP
                     DQLHAWFGATEDLAPRGFIMTKLGSLLVMANHLALRALSPTEQDIAQVLANEHVLVNV
                     VLYGDRVNFAADSYIVLEQAGRTVKPSTVRFDARGDRTTAWPQQPAYRAKVIAQFAYA
                     ELDPRAKTTLTVFPSRGGRVSFELDFSRIE"
     gene            2614..3321
                     /locus_tag="PK614_07210"
     CDS             2614..3321
                     /locus_tag="PK614_07210"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRB82037.1"
                     /translation="MSSPEQAPRRQYVNFTFYKIDPAWRRLPEDERTRGKQEFLRAVE
                     EYQGKVLVIAYTTVGIRGDCDLMLWRISYELELFQEMSTKIMASGLGKYLYNPYSYLA
                     ITKRSIYVDHHTHENQESKRLTVVPGKAKYIFVYPFVKTREWFLLTKAARQGMMDEHI
                     EVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTLRDTPI
                     FTCIRKSLKEALDTLGG"
     gene            3425..>4032
                     /locus_tag="PK614_07215"
     CDS             3425..>4032
                     /locus_tag="PK614_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82038.1"
                     /translation="MSTHAPSYAIPDRRAAIYSALLPGLGQLIRGRYGAGLFYGLVTI
                     LLILLSLALGRVSGRAAEVFFFMLLALPWWALQSYDAALGPPESGSDLARSTRTAWAQ
                     GHDIRFLGLLFLVSAGNDALLIARNPDYLLPFFCTRLDGSAGFITKALSPFLHTLAGY
                     GFLRVKKWSLLIYLVYAAYGTTNALVNLACFGPGRIRNTLLIA"
BASE COUNT          794 a         1342 c         1105 g          791 t
ORIGIN      
        1 cctcgaccgg caacatgccg atcgccaccg gccgctccaa caacccgttc tgagccagcg
       61 cctgtccgac ctgccgctcc ccttccttga tccgcgcgag cgtcaaatgc ggactgaatg
      121 gccgctcttc cggcgcgaag ttcgctgccc ggcaacaagc ctccaccgca cgatggaggc
      181 ttgtgagccg ttgcgcctca aggccctgct cccaactcac tgagggtccc gcccacagca
      241 cccgtggtgc ctgccggcgc gggaacgtac ccagtcgttc gagaggaata ggaatcgcct
      301 gatgcgctga cacggactct tcgatggcta cccgaagcgg ctcaatcgat tcgtcggctg
      361 tgtcgccgag aaatctgagg gtgaggtgca tcgaatccgg tcgaacccag ctgattctca
      421 catggcgatc gaccgttcgc tcgagttgtc gcttcatgtc ctgctcaatg gtggcaagcg
      481 tcgtgcgcaa ttcctgtggc aactcgatgg ccaggaatgc ccggaccatt agcacccctt
      541 cttgatcaac cagcgacgaa gcaaatcaag cgccgcctgc gaggaccgtt gcttgatcac
      601 ggtacggtca ccgccgtgaa aacgaaactc tttcgtcatc gcctcgcccg gcccgccgtc
      661 aagccccaca tagaccaggc ccaccggctt gctctcggtc gcgccaccgg gcccggcgat
      721 gccggtcacg ctcacgccga cggaggcgcc gctccgctca cgaatgcccc gcgccatcgc
      781 ggcggccacg tctgcgctga ccgccccatg ccgtttgagc agtcctgacg gcacacccaa
      841 catctcaacc ttggcacgat tgctataggt gacagccact cgatcaacat atgtcgatgc
      901 gcccggcacc tgcgtcagcc gatggccgat cagtccaccg gtacaggatt cggccacggc
      961 aagcgtcaac tgctgctcgt tgagcaatcg accgaccacc gattccatgg tctcatcttc
     1021 ttctccataa acgatgtcac cgagccgcgt cttggcttcc ttcaacaacg gctcgatggt
     1081 gacagccccc tccgctccag ccggtcccgt cagcgacacc atgacttcca ttggggacgc
     1141 atagatgccg agttgaatcg tgcaaccttt gggaacaagc ccgaccaggg cctggtccac
     1201 gattgattcc ggcacaccgg acgtgtggag cacccgccgg atcatgggcg acgcgctgag
     1261 cctcttctgt gtgcgcatcc acgcctgcaa ctgcggcaac acgccatcac gcaccatcgg
     1321 ttccatctca cgcggcaccc cgggcaaggc ggcgagaaac gtgcccttcc agacaagcga
     1381 aaaccccggc gccgttccga ccggattgaa gagaatctcc gcacgggcgg gaatcatggc
     1441 ctggcgaaac tgcgtggtgg tcggagttcg cccccattcg accagccgtg caatcatccc
     1501 atccagggca gccttgcgcc ggctcaagcg gtggcccgtc gcttgcgcca cggcttcccg
     1561 cgtgaggtcg tcgatcgtcg gccccaaccc accggtgaga atcaccacgc gggcgcgccg
     1621 taacgcggtc ttcaacacag ccacgatgtc ggacaggtcg tcgccgaccg tggtcttata
     1681 tcgaagctca attccacagg cggccaggcg atcggcaatg aagagcgaat tcgtgtcgag
     1741 gcggccgccg agcagcaact ccgatccgac ggcgatggtt tcagccgtcc aggttttggt
     1801 cttactcatg cgggatacac ctttgcgatg ggcagagcga tggagaggga ctgatactat
     1861 tcgatccgcg agaagtccag ttcgaagctg acccggcctc cgcgtgaagg aaataccgtg
     1921 agcgtcgtct tcgcacgtgg gtcgagttcc gcgtaggcga attgcgcgat caccttcgca
     1981 cggtaggcag gctgctgcgg ccacgccgtc gtgcgatcgc ccctggcgtc aaatcgaacg
     2041 gtggagggct tcaccgtccg accggcctgc tccagcacaa tgtaactgtc ggcggcaaaa
     2101 ttgacacggt ctccgtacaa caccacattg accagcacat gctcgtttgc cagcacctgg
     2161 gcaatatcct gttcggtcgg cgacaacgcg cgcagggcaa ggtgattggc catcaccagc
     2221 aggcttccca gcttcgtcat gatgaaaccg cgcggggcaa ggtcttcggt cgcaccgaac
     2281 caggcgtgca gctgatccgg agagacacct tggccggcag ccagctttcc acgctccacc
     2341 tgcgcctgaa tctgttcggt ggtcggctgc acctcgatgg catacgagga gccggtgctc
     2401 ccccacagga ccacgaagag gcttcccacg agccagaatc cgaccggcaa tcttcgaacg
     2461 ccgcgcatcg tatgcaggca gtatcagatc gatgtgggcc tgtcaattcg agccccgcgc
     2521 accccggact ctgttgacag ccccagagac gaaatgctaa agagctaact cttattcttt
     2581 tacattatca agaagttgcc tggagggttc gtcatgtcca gccccgaaca agcaccccgc
     2641 cgtcaatacg tcaattttac cttctacaag atcgatcccg catggcgtcg gcttcctgaa
     2701 gatgaacgca cacgcggaaa gcaggagttt ctccgtgccg tcgaagagta tcagggaaaa
     2761 gtgctcgtca tcgcttacac cacggtgggt attcgaggcg actgtgatct gatgctctgg
     2821 cgcatcagct acgagcttga actgttccag gaaatgagca cgaagatcat ggcatccgga
     2881 ttgggcaagt atctgtacaa tccctattcg tacctcgcga tcaccaaacg ttcaatttat
     2941 gtcgaccacc acacgcatga gaaccaggaa agcaagcggc tcaccgtggt ccccggcaaa
     3001 gccaagtaca ttttcgtcta tccgttcgtg aaaacccgcg aatggttcct cctcaccaag
     3061 gcggcgcggc agggcatgat ggacgagcac attgaagtcg gccatcgctt cccctcggtc
     3121 aagctcaaca ccacctattc cttcggtctc gacgatcagg aatgggttgt ggccttcgag
     3181 agcgacaagc cggaagactt cctggacctc gtcatggcgc tccgggaaac cgaaggtagc
     3241 cggtacaccc tgcgcgacac cccgattttc acctgcatcc gcaagagcct gaaagaagcc
     3301 ttggacaccc tgggcggcta ataacggttg cgcagcagtc tgtggacacg gccttcgatc
     3361 ccttgatcga aggccgtgcc gtttctatcc cgacacgccg tctgatctca cgcgttctcg
     3421 accaatgtcc acccacgccc cttcttacgc tatcccggat cgccgcgcgg cgatctactc
     3481 agccctactc cccgggctcg gacaactcat tcggggacga tatggggcag gactcttcta
     3541 cggcctggtc accattctcc tgatcctcct gagcctggcg ctcgggcggg tctccggtcg
     3601 agccgccgaa gtctttttct ttatgctgtt ggcccttccc tggtgggccc tgcagagcta
     3661 cgacgccgca cttgggccgc ccgagagcgg ttcggacctt gctcgcagca cccgcaccgc
     3721 ctgggcgcag ggacacgata tccggtttct cggacttctc tttctggtca gtgctgggaa
     3781 cgatgccctc ttgatcgcaa gaaatcccga ttacctgctg ccgttttttt gtaccaggct
     3841 ggacggctcg gccgggttca tcaccaaagc gctgtcaccg tttctccata ccctggccgg
     3901 gtatggcttc ttacgagtca agaaatggtc cctcctgatc tatctggtct atgccgccta
     3961 cggcaccacc aatgccctcg tcaatctggc ctgttttggt cccggccgga tccggaacac
     4021 actcctcatt gc
//
LOCUS       DAORQN010000129         4029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_812_length_4029_cov_2.956719, whole genome shotgun sequence.
ACCESSION   DAORQN010000129 DAORQN010000000
VERSION     DAORQN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_812_length_4029_cov_2.956719"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK614_07220"
     CDS             <1..581
                     /locus_tag="PK614_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRB82039.1"
                     /translation="TVPTIAASARIHPLATVIGSVTIGELIFIAPGASVRGDEGQNIY
                     VGDHSNVQDGVVIHGLETFEGDHELPENEVQVSGKSYSVYIGKRVSLAHQSQVHGPAR
                     IGDDTFVGMQALVFRTELGDHVVVEPGAKLIGVKVASGRYVPALTLVTTQEQADTLPL
                     ITSAYPYRNLNEGVVTVNVQLAKAGQPKQIGR"
     gene            complement(672..1745)
                     /locus_tag="PK614_07225"
     CDS             complement(672..1745)
                     /locus_tag="PK614_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRB82040.1"
                     /translation="MSRFVSALCGAVLALLAVLPGNAAAAPSSGSSWEPMRIMHFVLT
                     GTVPLERAREYVNQAQAAGFTAVQVVLTDGVRLDHAPWKPVKGAWTKAEFLSWAAYAR
                     AHGLDVIPEVKLLTHQEQFFQKHYPGLMFNAVSYDPRKEGVYQAVFRLLDELIDALHP
                     RAIHIGHDEAFGWTVGQVAKWLKFGEVMVPADLFARDVERIHGYLTQKGVETWMWADM
                     LLDPAEFPGASSKHLHGMAAGYGKALRNRLPRDIVMCDWHYGDEQGSFPSMAVMQSEG
                     FRVIGATWKREETMRNFSRYALSRHAYGLMATTWFHVQRNDRDLVDWIIRSSGMLFRN
                     PDAAVPPRPASAAPPEGHGWTAP"
     assembly_gap    2437..2502
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2503..>2746)
                     /locus_tag="PK614_07230"
     CDS             complement(<2503..>2746)
                     /locus_tag="PK614_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82041.1"
                     /translation="EQVFWERTPKAVRSLLNGKVWTVAFVNIDQRDWATRTRVLPEEK
                     AVIVGARDQRVQPAAILVNTYRTAAPDDPFYPDTQGR"
     assembly_gap    2747..2798
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2799..3659)
                     /locus_tag="PK614_07235"
     CDS             complement(<2799..3659)
                     /locus_tag="PK614_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82042.1"
                     /translation="MKTIGLHNEPALDCRQVSVLRPAAFLLRAGRIIMLSRAASWAIG
                     AGAVACALLFVPAVHANHQGHLSHSDGEAHTAVGAGHSIPGVSFQIFLGSGNDTAAET
                     GLTQADRSAALETVREAFAVLLQHRNDYPRFDESLKKEALARVVIETTVVNDEGRAFP
                     FLVARTAQPGRVTLLISASSLKENGYLHHPDTLAPVLAREFQWVASKADTAPKPKMVA
                     GERALKSAPIRTDQDIAALSGEERVRLLQQLLGSYLRTVDDQRSLDGQSYYEVGSTAL
                     VAPTHPDSTTK"
     gene            complement(3701..>4029)
                     /locus_tag="PK614_07240"
     CDS             complement(3701..>4029)
                     /locus_tag="PK614_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRB82043.1"
                     /translation="IHVVDLALGHLKALEALDRLERPGECLTVNLGTGNGYSVLEIVR
                     AFEAASGKPVPYKIAPRRPGDVASCYADPKQALKSLEWRAARGLKEMCADAWRWQSSN
                     PKGYAG"
BASE COUNT          823 a         1256 c         1076 g          756 t
ORIGIN      
        1 tcactgtgcc cacgattgcc gcatcggcac gcattcatcc cctagcgacg gtcattggat
       61 ccgtgaccat cggcgagctg attttcatcg caccgggcgc ttcggtccgc ggcgatgaag
      121 gacagaacat ttatgtcggg gatcacagta acgtgcagga cggagtcgtc atccatggac
      181 tggagacgtt cgaaggggat catgaattgc cggagaacga agtgcaggtc tcgggcaagt
      241 cctattccgt ctatatcggc aagcgcgtct cactggccca ccaatcgcag gtgcatggac
      301 ccgccagaat cggggacgat acctttgtcg gcatgcaggc ccttgtgttt cgcaccgaac
      361 tcggtgacca cgtggtggtc gaaccgggcg cgaagctgat cggggtcaaa gtcgcttcag
      421 gccggtatgt gccggcgctc accctcgtga ccacacagga acaagccgat accctccctc
      481 ttatcacctc tgcctacccc tatcgtaatc tcaatgaggg agtcgtgacc gtgaatgtgc
      541 aactagccaa agcggggcag ccgaaacaga tcggccgcta gcccggctca ccatgacaca
      601 gcggaaccgg caggcgttcc cctcgtgccg gggccctgac ggtcccactc aagagcgacc
      661 tttccccgcc gttatggagc cgtccagcca tgtccttccg gaggggcagc cgaagcaggc
      721 ctcggcggca ccgccgcatc gggattacgg aatagcatac cggacgagcg aataatccag
      781 tcgaccaagt cccggtcgtt ccgctgcacg tggaaccagg ttgtcgccat cagaccatag
      841 gcgtgccggg acaacgcata gcggctgaaa ttgcgcatgg tctcctcccg tttccaggtg
      901 gcgccgatca cgcgaaaccc ttcgctctgc atcacggcca tcgagggaaa actcccctgc
      961 tcgtcgccgt agtgccagtc acacatcacg atatcccgtg gaagccgatt gcgaagcgct
     1021 ttcccatacc ctgccgccat tccatgcaga tgcttggacg aggcgccggg aaactccgcc
     1081 ggatcgagca gcatgtcagc ccacatccag gtctccaccc ccttttgagt caggtagccg
     1141 tgaatgcgtt cgacatcccg ggcaaagagg tccgccggga ccatgacctc accgaacttc
     1201 agccatttcg ccacctggcc caccgtccat ccaaatgctt cgtcgtggcc gatatggatc
     1261 gcccggggat gcagggcgtc gataagttcg tcgagcagcc ggaatacagc ctgatagacc
     1321 ccttccttcc gcggatcata ggacacggca ttgaacatca aaccgggata atgcttctga
     1381 aagaactgct cctgatgtgt caggagtttg acctcgggaa tcacgtccaa tccatgcgcg
     1441 cgggcgtagg cagcccagga gagaaactcg gctttggtcc acgcgccctt cacgggtttc
     1501 cacggggcat gatcgagcct cacgccatcg gtgagcacca cctgcaccgc cgtgaaaccg
     1561 gccgcctgag cctggttcac atactcgcgc gcacgctcca gtgggaccgt cccggtcagg
     1621 acgaaatgca tgatccgcat cggttcccaa cttgagcccg acgatggtgc tgccgccgca
     1681 ttccccggca gaacagccag aagcgcaagc actgccccgc acagcgcaga cacaaaacgg
     1741 gacatgtctc ctcctcaatt gaacagcaga tccgtcttgc caagcctgag ctgcgccccg
     1801 tcgtcagccg cacattcact tcgggggaga gggtccgaac cagctcatgg cgacacagcc
     1861 gcgatgcatg ctgcgtagga gcggtgccat gcgcacctga tcccgcaaga cacagaccgg
     1921 tcaacgtcgc gcgatgccgt ccggataata cgttgagcca ggcaggggcg acagggcttc
     1981 gtccacaccg cacgtggccg atacatggcg atcccgcaac gcactctcac aaaaatcagg
     2041 ggggaaacct acacgcagac ttcacggagg tcaagaaaag ctctctggaa tatgcgcaaa
     2101 aatactgcgg ctgccccata cgtagagcaa accactacgc atatgcaatt tcgcactacg
     2161 tatgcatgcc agtacattct acttattgat cgattcgcgc cgaccaggcc cccaaaccgc
     2221 tcagacagga cgatctgtcg cagcaggatg ggaggcttga gatggacaga cgagcggaag
     2281 cgatgggtca aggggaagcg atgagacgct gggccagtcg attacggcac gatactcaat
     2341 ccctcgtttc gctccatccc tatttaggag ccgtaagtgc atcctgcgcc acgtggccgg
     2401 ccatcgattt ttcctggata ttatgctgaa tttccannnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgcccct gcgtgtccgg
     2521 ataaaacggg tcatcgggcg cagccgttcg ataggtattc accaaaattg cggccggctg
     2581 aacgcgttgg tcgcgcgctc ctactatgac ggccttctcc tcggggagta ccctggtccg
     2641 agtggcccaa tcacgttggt caatgtttac gaacgcgaca gtccacacct tcccattcag
     2701 caagcttctg actgccttcg gcgtacgttc ccagaacacc tgttccnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tggtcgtcga atccggatga
     2821 gtcggcgcga ccagtgcggt gctgccgact tcatagtagg actggccgtc gaggcttctc
     2881 tgatcatcta cggttcgcag gtacgagccg agtagctgct gaaggagacg tacccgctcc
     2941 tccccggaga gggccgcgat gtcctggtct gtgcgaatcg gcgccgactt caacgctcgt
     3001 tcacctgcaa ccatttttgg cttcggcgcg gtgtctgcct tgctggccac ccattgaaat
     3061 tctcttgcca gcaccggagc cagggtatcg gggtgatgca gatacccgtt ctccttcagt
     3121 gacgacgcgc tgatcagcag tgtgactcgt cccggctgtg cggttcgggc gacgaggaac
     3181 ggaaatgctc ttccctcgtc gttcaccacg gtcgtctcaa ttacgaccct ggccagcgcc
     3241 tctttcttga ggctttcatc gaaacgcgga tagtcgttcc gatgttgtaa gaggacagcg
     3301 aacgcttccc tcaccgtttc aagagcagcg ctcctgtctg cctgagtcag accagtctct
     3361 gctgcggtat cgttgcctga ccccaggaaa atctgaaagg acacgcctgg aatcgaatgc
     3421 cccgctccca ccgcggtatg agcctcgcca tcggaatggc ttagatgccc ttggtggttc
     3481 gcatgaaccg ccggcacgaa taagagagcg caagcgaccg ccccggcacc gattgcccat
     3541 gacgcagccc gggacagcat gatgatgcga cccgccctga ggagaaaagc agccggacgc
     3601 agaacgctca cctgtcgaca atcgagcgcc ggctcattat gtaggccgat cgttttcatc
     3661 cggagatccc ccgactcaca gcgcccaccc cgctgaacac tcagcccgca tacccctttg
     3721 gattcgaact ttgccaacgc caggcatcgg cacacatctc cttcaacccg cgcgcggccc
     3781 gccactctaa cgatttcaag gcttgcttgg gatcggcata acaggaggcc acgtcaccgg
     3841 gtcttcgggg ggcgattttg tagggaaccg gctttccact cgctgcttca aacgcccgca
     3901 cgatctccaa gacgctatat ccattccccg tcccgagatt caccgtcaaa cattcgccgg
     3961 gacgttccag tcgatcgagc gcttccagcg ccttgaggtg gcccagtgcc aaatcgacca
     4021 catgaatat
//
LOCUS       DAORQN010000130         4007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_816_length_4007_cov_7.113360, whole genome shotgun sequence.
ACCESSION   DAORQN010000130 DAORQN010000000
VERSION     DAORQN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_816_length_4007_cov_7.113360"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(336..902)
                     /locus_tag="PK614_07245"
     CDS             complement(336..902)
                     /locus_tag="PK614_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRB82044.1"
                     /translation="MVSDAPALLSRRGLLRAATAGMALAVVRLALPSVTWASRLPDGR
                     VSLYNLHTDERLSVTYRDESGAYDQDALNALNHFLRCHHTNETTTMDVQLIEFVNLVQ
                     KRVGGRREVHIVSGYRSPEYNEQLIRMGTRAARHSYHVSGQAVDVQIPGVPLRTLREV
                     ALRLGCGGVGYYPRGKFVHLDSGPFRHW"
     gene            complement(1232..>2253)
                     /locus_tag="PK614_07250"
     CDS             complement(1232..>2253)
                     /locus_tag="PK614_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB82045.1"
                     /translation="RGVMVGGGPEEFSGMLGTSASIHRIFDAIRKVSTNDAPILITGE
                     SGTGKELTARAIHERGLRQQGPFIPINCGAIPESLLESELFGYERGAFTGAVNQKKGK
                     VEFAQGGTLFLDEVGELPNALQVKLLRFLQDHTFERVGGHQPIEMNVRIIAATNVNLK
                     EAIEKGTFREDLYYRLGVVHINVPPLRERGEDVSLMAMAFLRQAAAHYDKHLEGFTRE
                     ALEAMRTYAWTGNVRELSNRVGRAVVMAEGTHVTPEDLDIPHQVARPDGSSISLKVNQ
                     QRIETDLIMKAFTLSQGNLSRAAQELGISRSTLYRRLRQYGMDQTLDARRALGVFPRA
                     SLSEH"
     assembly_gap    2254..2308
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2309..2647)
                     /locus_tag="PK614_07255"
     CDS             complement(<2309..2647)
                     /locus_tag="PK614_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB82046.1"
                     /translation="MAKPKVFVLTDDQAAVAAVRRSIESECEVFVAAWDRTAVVQIHE
                     QAPLVMLLDLGSSSDPAVEERVRVLQEVRQTGHAGKVIACTERTERRLAVRAIQYGAY
                     DVLSKPLDITL"
     gene            complement(2750..3139)
                     /locus_tag="PK614_07260"
     CDS             complement(2750..3139)
                     /locus_tag="PK614_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82047.1"
                     /translation="MEQPTGSSGQRSGKRPTVAVVYEDVTTGYKCADWFALHGYQATM
                     TSAVHHLERNLKELRPDVIVVRLSSAPTPLHSTVPRLKMTCPQVPIIAMVDQSTDALD
                     GVAPDLTKAFGADVFMCDSLDPPSPPS"
     gene            complement(3533..>4007)
                     /locus_tag="PK614_07265"
     CDS             complement(3533..>4007)
                     /locus_tag="PK614_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB82048.1"
                     /translation="DISMPGRSGPEVLKELKQLAPTLPVLILSMHPEDQYAIRMFKAG
                     AAGYLTKASAPEELVHAAKKVMSGGQYVSASVGEALALTVRTGVDKLAHERLSDREYE
                     VLCLIASGQTVSDIAESIHLSVTTISTYRARILDKMNLKNNAELTRYALQHGLVV"
BASE COUNT          853 a         1249 c         1047 g          803 t
ORIGIN      
        1 ggcgaatggt gtactcgact gaaacgatat cgcccttaga taccttcatc attcagtccc
       61 cctcaccggc agtccccatg ggttgccatc acttccgctt cggcccatct cagtccgcca
      121 tgtcctgcag cctccgctcg tgttcgggat aacagacggc aatgcgctag agacggcatc
      181 cagaagcacc ctatcacgac gggatcatgg ggtcatggga atagcaggaa tgcccctcat
      241 gccggcaggt gatcggacct atacaccgag agcaggcgat ccggtgatgc tctgagaagc
      301 gccgtgaacg aaaagggaac ggcaagaact ggtcgttacc agtgacggaa gggacctgaa
      361 tcgagatgga caaacttgcc acgcgggtaa tatcccaccc cgccacaacc gagccgtaac
      421 gccacctcac gcaaggtcct cagcggcaca ccgggaatct gcacatccac ggcctgcccc
      481 gacacatggt agctgtgacg cgccgcacgc gtgcccattc tgatgagctg ctcgttatat
      541 tctggagacc ggtaaccgga tacgatatgc acttcgcgac ggccgccgac ccgcttttgg
      601 acaagattga cgaattcgat gagctgcacg tccatcgtgg tggtttcgtt cgtatggtgg
      661 cagcgcagaa aatgattgag cgcgttgagt gcgtcctgat cgtacgcgcc agactcgtca
      721 cggtacgtca ccgacaaccg ttcatcggtg tgaagattgt acaaactcac ccggccatcg
      781 ggtaaccgtg acgcccacgt caccgacggc agggccaggc gaaccacagc cagcgccatc
      841 ccggccgtag cggcgcgcaa caatccgcgc cggctcaaga gggctggtgc atcggagacc
      901 atcgattgac tcccactcga aaacatactt accatttcgc gccgtcagca cgtgattgac
      961 aaggctggac ccgtagattt ctcaacctgt catgcgtgaa acaaccaatt cacgcgtaca
     1021 ttgtcacagg atgggccaca agccgaggtg aggaaccgta ccccggagaa cagcatgatc
     1081 agcaggtaat cggagactgt tgtatgtacc aaatacctgt acgacggtca accgttttac
     1141 acacctatgc actcgcatcg atctcagagt gacaggattg tatcactacg gcacaccagc
     1201 cacgatcaat ctcacagtgc cggccgcatc atcaatgctc tgacagcgag gcgcgcggga
     1261 acaccccgag cgctcgccgc gcatcaagcg tctggtccat cccgtactgg cgcagccgtc
     1321 gatacagcgt cgaacgactg atgcccaatt cctgagcggc tcgactcaag tttccctgcg
     1381 acagcgtgaa cgccttcatg atcagatcgg tctcgatccg ctgttgattc accttaagcg
     1441 aaatcgagct gccgtccggt cgcgccacct gatgcgggat atccagatcc tccggagtca
     1501 cgtgcgtgcc ttctgccatc accaccgcac gaccgaccct gttggacaat tcacgcacat
     1561 tccccgtcca ggcataggtg cgcatcgcct ccagcgcttc gcgggtaaaa ccttccagat
     1621 gtttatcgta atgggctgcc gcctgacgca ggaacgccat ggccatcaac gacacatcct
     1681 caccgcgctc ccgaagcgga ggcacattta tatggacaac cccgagccga tagtagaggt
     1741 cctcacggaa cgttcctttt tcaatggcct ccttcagatt cacattcgtc gcggcgatga
     1801 ttcgcacgtt catttcaatc ggctgatgcc cgcccacccg ctcaaacgtg tgatcctgca
     1861 gaaaccgcaa caatttgacc tgcaaggcat tcgggagttc gcccacttca tcgaggaaga
     1921 gcgtcccccc ttgggcaaat tcaaccttgc ctttcttctg gttcaccgct cccgtaaagg
     1981 cgccacgctc atacccgaag agctccgatt ccagcagact ttccggaatc gccccgcaat
     2041 tgatgggaat gaacggtccc tgctgccgta accctctctc gtggatcgcg cgcgccgtca
     2101 gctccttgcc tgtgccgctt tccccggtga tcaagatcgg tgcatcgttc gtggaaacct
     2161 ttcgtatggc gtcgaagatc cggtgaatgc tggcactggt gccgagcata ccgctgaatt
     2221 cttcagggcc tcctccaacc atgacgcctc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa gggtgatatc cagcggttta gacaacacgt
     2341 cgtaggctcc atactggatc gcgcggacgg cgaggcggcg ctccgtcctt tccgtgcagg
     2401 cgatgacctt tccggcatgc ccggtttgtc gcacttcctg cagaacccgc acacgctcct
     2461 cgacagcggg atcggacgag gaccccaaat ccagaagcat gaccagcggg gcctgttcgt
     2521 ggatctgaac caccgccgtc cgatcccatg cggccacgaa cacctcacac tcgctctcga
     2581 tcgagcggcg cacggcggca acggctgcct gatcatctgt gagaacgaat accttgggtt
     2641 tcgccacgat tcggctcctt tcagctggtg agacactggc acgatgggac ggctgaccat
     2701 cgtcgcactg tctatgctag gcggtgtctg agcaagtcag gcgtgcgtac tacgagggcg
     2761 gcgacggcgg atccagcgaa tcacacataa agacatcagc accgaaagcc ttggtcagat
     2821 caggagccac cccatccagg gcatccgttg attgatccac catcgcaata atcggcactt
     2881 gcgggcaggt cattttgagt ctgggaacgg tactgtgcag cggcgtcgga gcggacgaca
     2941 atctcaccac aatcacatcc ggacgaagtt ccttcaggtt gcgctccagg tgatggactg
     3001 ccgacgtcat ggtggcttgg tacccgtgca gcgcaaacca atcggcacac ttgtagccgg
     3061 tcgtgacgtc ttcgtacacc acggcgaccg tgggtcgttt cccggatcgc tgtccgctgc
     3121 taccagtcgg ttgctccatc agttcaccct cgtctggatc agcttaggtt gtggttcgaa
     3181 cgcctgtgag ttgtgtcaat cgacgcgcca tttgaggacc ccaccaggcg aggtcttgta
     3241 acatcgcggc ttccacggcg gtcaatccgg tgaggatgcc gggccaaccc gggcgaaagg
     3301 acccggttcg gcgatcaata ccagaacgct ctgccgatcc ctgtccctgc gcagcctcaa
     3361 agaccgcaag caatgtcgtc gtagtggaga tccacggctt ctgcatgaag atgaggatag
     3421 gcccgagaag aggaggatgc tatcggcaga ggtgcgattc ctgcgtcaca aatagacagt
     3481 gcctcaatgt catggttata acgacatggg cgaggcgacc gaccccattc ccttatacga
     3541 ccaacccatg ctgcaacgca tagcgcgtca actcggcatt atttttcaaa ttcattttgt
     3601 ccagaattct cgctcgatac gtactgatcg tcgtcacgct gagatgaata ctttcggcaa
     3661 tgtcactgac ggtctgtccg gaagcaatca gacagagcac ttcgtactcc cggtccgaca
     3721 gccgttcatg ggccaacttg tcgaccccgg tcctgaccgt caacgccaag gcttctccca
     3781 ccgaagcact gacgtactgc ccgcccgaca tgactttttt cgctgcatgg accagctctt
     3841 ccggcgcact cgccttcgtg agataccctg ccgcgccggc cttaaacata cgaatggcat
     3901 actgatcttc cgggtgcatg ctcaagatca agaccggcag cgtcggcgcc agttgcttca
     3961 gttccttcaa cacttccggc ccgctccgtc ccggcatact gatgtcc
//
LOCUS       DAORQN010000131         3997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_820_length_3997_cov_5.716895, whole genome shotgun sequence.
ACCESSION   DAORQN010000131 DAORQN010000000
VERSION     DAORQN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_3997_cov_5.716895"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="fliM"
                     /locus_tag="PK614_07270"
     CDS             complement(<1..782)
                     /gene="fliM"
                     /locus_tag="PK614_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRB82049.1"
                     /translation="MDKILSQEEIDALMGGVMSGEVETASPEEVDPGGVKAYSLTSQE
                     RIIRGRMPTMEMINDRFTRQQSISWSAVLREKIEFSVLGAQIMKFGDFIHKIPVPSSF
                     NLFAMEPLRGNGLYVMDAMLVYLLVDFFFGGKVQTHVKPEGREFTTIQLRLIKKLVQQ
                     ALADLEKAWQAVMPVKIGYLRSESNPQFAMVVTTSEIVVVVTLQVHLGDLSREMFIAY
                     PYSMLEPIKEKLYSGLFADNVEQDSSWGSRFKDSLQECDVALT"
     gene            complement(807..1343)
                     /locus_tag="PK614_07275"
     CDS             complement(807..1343)
                     /locus_tag="PK614_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRB82050.1"
                     /translation="MSEAAEEKAPAASAGIPMKMVIIIVAGVLVLALGGAFAMFKMMG
                     GPSGEKAQGEGLGEKPSAHGDTVAKEGVHAAGGETGAIADLDPFIVNLADSPEVRYLK
                     VTMKLEMESLGAVEDVKARTPQIRDAILVLLSSLDSVTARSPQGKYKLREDVTQRING
                     LLPKKPVKMTYFTEFVIQ"
     gene            complement(1677..2900)
                     /gene="flgE"
                     /locus_tag="PK614_07280"
     CDS             complement(1677..2900)
                     /gene="flgE"
                     /locus_tag="PK614_07280"
                     /inference="COORDINATES: protein motif:HMM:NF004238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRB82051.1"
                     /translation="MGILTSMFTAVTGLSSYGNAMGVIGNNIANVGTAGFKSSRATFS
                     DLISSSLAGGSGGDQVGLGVYMNDVQTNFTQGSITTTGNTFDLAIDGNGFYLLRDSAG
                     ANFYSRAGQFKVDNIGQVVDSAGALLQGYQADTNGNITSTIGSITLSSSAVAPQTTTT
                     AAILGNLNANATAPTTAFSVSDTTSYNFATGMTFYDSLGNSHQMQLYFRKTAANTWGV
                     YSQIDGGTAAAQTNMTFNASGALTAGGTQTVTAALTNGATTPQAVTVSLAAMTQFGSA
                     SGIISQTQDGFASGTLDRISVDKQGHVVAQFTNGQTRPLAQVVLNRFLNPDGLVSNGD
                     NHFLETIESGAPLSGAPTTNGLGRILSGTIEQSNVDLGKEFVDMIITQRAFQANSRAI
                     TTSDEMLQELVNLKR"
     gene            complement(2960..3265)
                     /locus_tag="PK614_07285"
     CDS             complement(2960..3265)
                     /locus_tag="PK614_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRB82052.1"
                     /translation="MDISKVITQATPPDSSTPTTDGPKPLGKDDFLKLLVTQLQHQDP
                     LKPMENEDFVAQTAQFSQLEQMAKLVSLMEKSVTLQENAQNKTATTGTTAVAYRSSL"
     gene            complement(3277..>3997)
                     /locus_tag="PK614_07290"
     CDS             complement(3277..>3997)
                     /locus_tag="PK614_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRB82053.1"
                     /translation="MKQADGDRVDRPAFLEDLAQDSPVSTEQGSANAGRFLENESEPF
                     SDSSADADRSMPNQGMSNGPTDRPLFLDRMSGLNQPAPPPADSAVGRNEAGQPAAVLR
                     AAESQRMSELRGASPVAHSVMLDLDPLDMGPLRVRVMMSDQTVHAHIRTEHGELGQGL
                     LQQGQSLESSLRTTGLEMGMLRVTVDQQQGRSDNAWMFQQQHQDRPTTSSGQRSTTGE
                     EERSARGETGDYTNERVSIFA"
BASE COUNT          786 a         1182 c         1108 g          921 t
ORIGIN      
        1 gtcagagcca cgtcgcattc ctgtaaggaa tccttgaaac gactgcccca actgctgtcc
       61 tgctcgacat tgtcggcaaa caaaccggaa tacaatttct ccttgatcgg ctccaacatg
      121 gagtagggat acgcgataaa catttcgcgg gagaggtcgc ccaggtgcac ctgcaacgtc
      181 accaccacga cgatttccga ggtcgtcacg accatagcga actgcgggtt gctctccgat
      241 cgtaagtacc cgatcttcac cggcatcacg gcctgccagg ctttctccag atcggccaac
      301 gcctgctgaa ccagtttctt gatcaggcga agctggatcg tcgtgaactc cctaccttca
      361 ggcttcacgt gtgtctgcac ttttccgccg aagaagaagt ccacaagcag gtacaccagc
      421 atggcgtcca taacgtagag tccgttgccg cgcaacggct ccatggcgaa cagattgaag
      481 gaggagggaa ccgggatctt gtgaatgaag tccccgaact tcatgatttg agcgccgaga
      541 acgctgaatt ctatcttctc gcgaagcacg gcgctccagg atatggattg ctgacgcgtg
      601 aagcgatcgt tgatcatctc catcgtgggc atacgcccgc gaatgatgcg ttcctgactg
      661 gtgaggctgt acgctttaac cccgcccgga tctacctcct cgggcgaagc cgtctcgact
      721 tcgccggaca tgacgccgcc cattaaggca tcgatttctt cttgagagag tattttatcc
      781 atggcatctc gacggacgac gatcgactat tgaatgacga attccgtgaa gtacgtcatc
      841 ttgactggtt tctttggaag gaggccgttg atccgctgcg ttacgtcttc gcgcagcttg
      901 tatttaccct ggggagaacg tgcggtcaca gaatcgagac tgctcagcaa caccaggatc
      961 gcatctcgaa tctgcggcgt gcgggccttg acatcttcga cggcgccgag gctttccatt
     1021 tcgagtttca tcgtcacttt gaggtaccgc acttccggcg aatcagcgag attgacgata
     1081 aaaggatcga ggtcggcaat cgcccccgtc tcgcctcctg cagcgtgcac accttccttg
     1141 gcaacagtgt ctccgtgagc ggacggtttc tcccccaaac cttctccttg cgccttctcg
     1201 ccggagggcc cgcccatcat cttgaacatc gcaaaggcgc cgcccagcgc gagaaccaat
     1261 actcccgcga cgataatgat gaccatcttc atgggaatac ccgcggacgc tgcgggcgcc
     1321 ttctcctcag ctgcttctga catacggacc tcctgcggac agtgtttgtc ggtggtggtc
     1381 ggttgtgcaa tgcatatgcc acgcaccgac cgacagcagg cctctacaga tttcgctctc
     1441 tgaaccggcg cctgcgataa ctcactgatt tcaggccacg atgtcttccg gaaacgcggg
     1501 atcaaggacg gggcgtgggc cgttgtcagc agagtgacgc gggtttctcg agagccgaca
     1561 gaatcttgac gaaggtgaga aaccgtgagg ggatgatgtg tgggtgaccc gctaggcaga
     1621 aactgccgac ttgagccctg acgtgcgctt tctacgcgcg tcagggctct cctcgcttac
     1681 cgtttgagat tcacgagttc ctgcaacatt tcgtcgctgg tcgtgatcgc gcgtgagttg
     1741 gcctgaaagg ctcgctgggt aatgatcatg tcgacgaact ctttgccgag gtcgacattc
     1801 gactgctcga tggttccgga caagattctc cccaagccat tcgtcgtcgg cgcaccggat
     1861 aacggcgcgc cagactcaat ggtttcaagg aaatgattgt caccgttgct gaccaaacca
     1921 tctggattca agaaacggtt cagaaccact tgcgccagag gtcttgtctg cccattggta
     1981 aactgagcca ccacgtgtcc ctgcttgtcg acgctgatgc gatcaagtgt tcctgaggca
     2041 aatccatcct gcgtctgact gatgataccg gacgcggacc cgaattgagt cattgcagca
     2101 agactcaccg tgacagcttg aggtgtcgtg gcgccgttgg tcaaagcagc ggtcacggtc
     2161 tgtgttcccc cggccgtcag cgctccactg gcattaaacg tcatgttggt ttgcgcggca
     2221 gccgtgccac cgtcgatttg ggagtacact ccccaggtat ttgccgccgt cttccggaaa
     2281 tacaattgca tttgatggga gtttccgagg gaatcataga acgtcatccc ggtcgcgaaa
     2341 ttgtaagatg tggtatcgga cacactgaac gccgtcgtcg gcgccgtggc attggcattc
     2401 aggttgccga gaatcgccgc cgtggtggtc gtctgtggcg cgaccgcact ggacgacagc
     2461 gtgatgctgc cgatcgtgct cgtaatgttg ccgttcgtat cggcctggta gccctgcagt
     2521 agggcacctg ccgaatcgac gacttgcccg atattgtcga ccttgaactg accggcacgg
     2581 gagtagaagt tcgcgccagc actgtctcgc aggagataaa atccgtttcc atcgatcgcc
     2641 agatcgaatg tgttcccggt ggtggttatt gatccctgtg taaagttggt ctgaacatca
     2701 ttcatataca ctccgaggcc gacttgatcg ccaccgcttc ccccggccag gctggaggag
     2761 atcaagtccg aaaaggtcgc ccggctggac ttaaaacccg cagtcccgac attggcgata
     2821 ttgttcccga tcacccccat ggcgttgccg taggagctca agccggtcac tgccgtgaac
     2881 atcgatgtta aaatgcccat tgtctcatcc tccttgttac cacgacctga aacgttctct
     2941 ccacgcgttc gcccgcacct cagagcgatg agcgatatgc gacggccgtc gtgccggtag
     3001 tcgcagtttt attctgcgcg ttttcctgaa gcgtcacact cttctccatc aaggacacca
     3061 gcttcgccat ttgttccagc tgcgagaact gcgcggtctg cgcgacgaag tcctcatttt
     3121 ccatcggctt cagcggatct tgatgctgaa gctgggtgac gagcagcttc aggaaatcgt
     3181 cttttcccag cggcttcgga ccatcggtgg tcggcgtgga tgagtctggg ggtgtcgcct
     3241 gtgtaatcac cttgctgata tccataggac ctccaatcat gcaaatatac tgacgcgttc
     3301 attcgtgtag tcgccggtct ctcccctcgc actgcgctct tcttctcccg tcgtggaccg
     3361 ctggccggac gaggtcgtcg ggcgatcctg atgctgctgc tggaacatcc aggcattgtc
     3421 gcttcgcccc tgttgttgat cgacagtcac ccgcaacatg cccatttcca gacctgtcgt
     3481 gcggagcgat gattccagtg actgtccctg ttgcagcagg ccttgtccca gttctccatg
     3541 ttccgtccgg atatgcgcat ggaccgtttg gtcgctcatc atgacacgga cccgcaaggg
     3601 gcccatgtcc aggggatcca agtccagcat gacgctgtgc gccacaggcg acgcgcctcg
     3661 aagttcgctc atgcgttggg attcagctgc tcgcaggacc gcagcgggct ggccggcctc
     3721 gttgcggccg acggcgctgt cggcgggagg cggggccggt tgattcagtc cactcatccg
     3781 gtccaggaac aacggacgat cggtcggccc gttgctcatg ccttgattcg gcatcgagcg
     3841 atcggcatcg gcagaagagt cggaaaacgg ctctgactca ttctcgagaa atctcccggc
     3901 gttcgcactt ccctgttcgg tgctcaccgg gctgtcctgt gcgagatctt caagaaatgc
     3961 cggccggtca acccgatcgc cgtcagcctg tttcatg
//
LOCUS       DAORQN010000132         3924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_830_length_3924_cov_5.756268, whole genome shotgun sequence.
ACCESSION   DAORQN010000132 DAORQN010000000
VERSION     DAORQN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_830_length_3924_cov_5.756268"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PK614_07295"
     CDS             <1..273
                     /locus_tag="PK614_07295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRB82054.1"
                     /translation="HAKAPYLLAPAAIGLAYLLFLLVRSPFGGSRGDASPAAILLNGD
                     AHAIGIKMFSEYLLQFEIIGVFLTGAIVGAIVLAKTPKSIETRRDA"
     gene            270..572
                     /gene="nuoK"
                     /locus_tag="PK614_07300"
     CDS             270..572
                     /gene="nuoK"
                     /locus_tag="PK614_07300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRB82055.1"
                     /translation="MIPLSAYVAVSAVLFATGLLGVLIRRNFIIVLMSVEIMLNAATI
                     NLVAFSHYLEAMQGQIVALFIIAIAAGEAAIGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            574..2577
                     /gene="nuoL"
                     /locus_tag="PK614_07305"
     CDS             574..2577
                     /gene="nuoL"
                     /locus_tag="PK614_07305"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRB82056.1"
                     /translation="MSDSIDLIVKLIPLFPLMAVIVNGLFGHRYSHDLAHRFAWGSVL
                     MSFLCVLAVFTEILRTGAAREVVAYKWIFGGDLTINLAYLIDPLTCIMLLVITGVGFL
                     IHVYSVGYMHGESGFTRFFVYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGY
                     YYDKVSAAKAATKAFVVNRIGDAGFLLAIFFFFVKFRTLDYTQVMQNAAQLSPEMATA
                     IALCLLIGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDL
                     SPTAMTTVAWIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGLGAYTAAVF
                     HLMTHAFFKALLFLSAGSVIHALSGEQDIRKMGALKSKIPWTHTLFLIGTIAIAGIPP
                     LAGFWSKDEIMGHAFVHHHYVLYGMAAVGAFLTSFYMFRLTYLTFYGTSRLDHHTAEH
                     VHESPMVMIGPLVVLATLSVIGGFPGVPPENGWFHHFLHSVAGVAGEEHATAPALMLG
                     LMGTATVIALLGWGLAHYFYSGPSSAADALAARMPGVYTTLLNKYFVDELYDRLFVEP
                     TKQIGRLCDWFDRTIIDGLVRSIDRGTDASSAAITWTEKHVVYAGLNIIGYANHRLAR
                     SWRRLQTGMVHQYAAIIVAGLFILVHLILLIWTGSGSNGYLSR"
     gene            2602..>3125
                     /locus_tag="PK614_07310"
     CDS             2602..>3125
                     /locus_tag="PK614_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRB82057.1"
                     /translation="MLEEFSFGFPILSYLIFLPLAGAAVLWLIEDEDLLKTTTLGITL
                     VELALACLVLVRFVPDSAAMQFAEHARWMTALGIGYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTVRNQVRLYFMALLALETTTMGIFASIDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYT"
     assembly_gap    3126..3165
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3166..>3924
                     /locus_tag="PK614_07315"
     CDS             <3166..>3924
                     /locus_tag="PK614_07315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRB82058.1"
                     /translation="LDINYHQWAVTHHLDHVYSFDLLELLSVPIPLSQQILIFWLMFM
                     GFAFKAPVFPFHTWLPDALVEGPIGMAVMLAGMKLGTYGFLRFTFPLLPDASKSEGVV
                     SIVMVLALAAILYGAVVALIQSDFKRLLAFSSISHLGFVVVGLFALNFQGLQGSLLTM
                     INLGFSTAGLFFMAGFLSTRQQSSQLSSFGGFAKQVPLLASFLLLIGMASIGLPGTNG
                     FVGEFLILLGAFKAKWWYGAVGVTGVIFGAAYFLW"
BASE COUNT          681 a         1180 c         1043 g          980 t
ORIGIN      
        1 cacgccaagg ctccttatct tctcgcacca gcggccatcg gtttggccta cctcctgttc
       61 ttgttggtga gatccccgtt cggcggctcc aggggagatg cctcaccggc ggcgatcttg
      121 ctgaacggag atgctcatgc catcggcatc aagatgttca gcgagtattt gctgcaattt
      181 gaaatcatcg gggtgttcct gacaggagcc atcgtcggcg cgatcgtcct cgcaaagacc
      241 cccaagtcga tcgaaacgag gcgcgacgca tgattccctt gtccgcctat gtggctgtaa
      301 gtgccgtgtt gtttgcgacg gggttgctcg gcgtcctgat ccgtcgcaat tttattattg
      361 tgctgatgtc ggtcgaaatc atgctgaacg cggccaccat caacctggtc gccttctccc
      421 attacctgga ggccatgcag ggacaaatcg tggccctctt cattatcgcc attgcagcgg
      481 gggaagcggc tatcgggctc gccatcatca tcgtggtgtt ccgcggcaag atttcaacca
      541 acgtcgacga aatgaatttg ttgaagtggt aacgtgtccg actctattga tctgatcgtc
      601 aaactgattc ctctgttccc gctgatggcc gtcattgtga acggcctctt cgggcatcgg
      661 tattctcacg acctagctca ccggttcgcg tggggatcgg tcctcatgtc tttcctctgt
      721 gtgctggccg tcttcacgga gatcctgcgt acaggcgcgg ctcgtgaagt ggtcgcctac
      781 aaatggattt tcggcggcga cctcacaatc aatctcgcct atttgatcga tcctttgacc
      841 tgcatcatgc tgctggtcat caccggagtg ggattcctga tccacgtcta ctccgtggga
      901 tacatgcacg gagaatccgg cttcacgcga ttctttgtgt acatgaacct ctttatggtt
      961 tcgatgctcc tgttggtgat gggcaataac tacgttgtcc tctttatcgg ctgggaaggc
     1021 gtcggactct gctcctatct gttgatcggg tactactacg acaaggtgtc cgccgccaaa
     1081 gcggccacca aagcctttgt ggtcaatcga atcggtgatg ccggatttct cctcgcaatc
     1141 ttcttttttt ttgttaagtt ccgaaccctc gactacaccc aggtgatgca gaacgccgca
     1201 cagctctcgc ctgaaatggc cactgcgatc gcactctgcc tcttaatcgg cgcggtcggc
     1261 aaatcggcgc agctcccgct ctacacctgg ctaccagacg ccatggaagg gccgaccccg
     1321 gtcagtgcgc tcatccatgc ggccaccatg gtcacggcag gcgtctatat gatcgttcgc
     1381 aatcatgcca ttttcgatct gtcgcccacc gccatgacga ccgttgcctg gatcggcgga
     1441 ctcaccgcgc tcttcgccgc tacaatcggc ctggtacaga ccgacatcaa gcgcgttctg
     1501 gcctattcca ccgtcagcca gctcgggtac atgttcctcg gttgtggtct cggagcgtat
     1561 acggccgcgg tcttccatct gatgacccat gcgttcttta aagcgctctt gttcttgtcg
     1621 gccgggtccg tcatccacgc attgtccggc gagcaggata tccgcaaaat gggcgcgtta
     1681 aagtcgaaga tcccctggac ccatacactc ttcctgatcg gcacgatagc gattgccgga
     1741 attccgccct tggcgggctt ctggagcaaa gacgaaatca tgggccatgc cttcgtgcat
     1801 catcactatg tgctctacgg catggcagcg gtcggcgcat tcctgacctc gttctatatg
     1861 ttccgcctga cctatctcac gttctatggc acctcgcggc tggatcacca caccgccgaa
     1921 cacgtgcacg aatcccccat ggtgatgatc gggcccctcg tcgtgctggc caccctctca
     1981 gtcattggcg ggttccccgg tgttccgcct gaaaacggct ggttccacca cttcctgcac
     2041 tcagtcgcag gtgtggccgg ggaggagcac gccaccgcgc ccgcgctgat gctcgggctg
     2101 atgggcacgg ccaccgtcat tgcgctcctg ggctggggac tggcgcatta cttttacagc
     2161 gggccttcct ccgcagcaga tgcgctcgcc gcacggatgc cgggcgtgta tacaaccctg
     2221 ctgaacaagt actttgtcga cgaactgtac gaccggctgt tcgtggaacc gaccaagcag
     2281 atcgggagac tttgcgactg gtttgaccgg accatcatcg acggcctcgt ccgatctatc
     2341 gatcggggga cagacgccag ttcggcggcc atcacctgga ccgaaaaaca tgtggtctat
     2401 gccggcttga acatcatcgg ctatgcgaac caccgcttgg cccgctcgtg gcggcggctt
     2461 cagaccggca tggtgcatca gtacgcggct atcattgtgg cgggactttt tattctcgtg
     2521 cacctcattc ttttgatttg gaccggaagc ggttccaacg gctatttatc acgctgacgc
     2581 agcagaattt ctggacacac gatgcttgaa gagttcagtt ttggcttccc gatcctctcg
     2641 tatctgatct tcctcccgct cgccggcgcg gcggtgctct ggttgatcga agacgaagat
     2701 ctcctcaaga cgacgacatt gggtatcacc ctggtggaac tggccctggc ctgcctggtg
     2761 ctggtgcgct tcgtgccgga ttccgctgcg atgcaattcg ccgagcatgc gcgctggatg
     2821 acggcgctcg gcatcggcta ccatctcgcc gtcgacggca tcagcgtgct ctttgtaggg
     2881 ctgactgcgt ttctcaccgt gctggtcgtc atctattcct gggataccgt acgcaatcag
     2941 gtccgcctgt acttcatggc gctcttggcc ctagagacca ccaccatggg tatctttgcc
     3001 tcgatcgacc tgattctgtt cttcgtcttc tgggaactca tgctcatccc gagctacttc
     3061 ctgatcaaat tgtggggcgg aggagcagag cgccattacg cagccctcaa gtatgtgctc
     3121 tacacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgga catcaactat
     3181 caccagtggg cggtgactca ccacctcgac cacgtctact cattcgacct gctggagtta
     3241 ctctcggtcc cgatcccgct ctcccagcag attttgatct tctggctgat gttcatggga
     3301 ttcgccttca aagcgccggt gttcccattt cacacctggc tccctgatgc cctcgttgaa
     3361 ggacccatcg gtatggccgt catgttagcc gggatgaaat tgggtaccta tggcttcctt
     3421 cgttttacct tcccgttact tcccgatgca tcgaaaagcg aaggcgtcgt ctcgatcgtc
     3481 atggtcctgg ccctcgcggc aattctgtac ggagcggtgg tggcgctcat tcaatcagac
     3541 ttcaagcgcc tcctcgcatt cagcagcatc agccaccttg ggtttgtggt ggtcgggctc
     3601 tttgctctga atttccaggg cttgcagggg agcctcctga ccatgatcaa cctcggtttc
     3661 agcaccgccg gcctcttctt catggcaggc ttcttatcca ccagacaaca gagttcccaa
     3721 ttgtcctcct tcggaggatt cgcgaaacag gtaccgctgc tggcctcgtt cctcctgttg
     3781 atcggtatgg cctccatcgg actgcctggg accaatggtt ttgtgggtga gtttctgatc
     3841 ctcctcggcg ccttcaaagc caaatggtgg tatggcgccg tgggggtcac cggcgtcatc
     3901 ttcggagcgg cctatttctt gtgg
//
LOCUS       DAORQN010000133         3853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_845_length_3853_cov_8.536598, whole genome shotgun sequence.
ACCESSION   DAORQN010000133 DAORQN010000000
VERSION     DAORQN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_3853_cov_8.536598"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="PK614_07320"
     CDS             <1..2206
                     /locus_tag="PK614_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB82059.1"
                     /translation="DIPYDTTRFIDVSIKEVVKTLAEAMVLVILVVYLFLQSWRATLI
                     PGVAVPVSLVGTFAGMQALGFSINTLTLFGMVLAIGIVVDDAIVVVENCERHMTQGGL
                     SAKNAAKRAMEEVTGPVIAIVLVLCAVFVPVGFLGGITGELYKQFAITISIAVIISGF
                     VALTLSPALCALVLKPGEEQHHGIFGLFNRFFSWMQLRYISTVGLALTRRVLSVAVFA
                     VLLIGVFMLFRVIPSSFLPEEDQGYFITIVQLPDGASKQRTDAVLSKIESYFLASPVI
                     HSTDALSGQNFVFNTRGPNAATMFVPLKHWDERAAPQHHVKALIGAAYGEFAKIPEAL
                     ILAFNAPSIRGLGATGGFTLQLQDPSSGDFNKFSAAAQEFVGKARQNPAIGAIGTSFR
                     VSAPRILATVNRERAKALGVPISEVFDTMQAYFGNFYINDFIKFGRVFRVQTEADAQY
                     RSTPEDISKIYVRASGPQGTTMIPLDTVVSTEFTSGPDPVTHFNGYNTALVLGAAAPG
                     FSSGQVLDALEQVAKEVLVPQGYGIDWSGISYQERMVGSQSLYAFAFGLLMVFLVLAA
                     QYESWVVPFAVILAVPIGLFGALSAVWLKGMTNDIYFQIGLVTLIGLSAKNAILIVEF
                     ANKRFEEGHPLLEATIEAAKLRFRPIVMTSMAFILGVVPLVIATGAGAASRNSIGTGV
                     FGGMLAATFLAIFFVPLFFVLIRSLSRRLKKPTPPAQDAPDDPEKERDYQHA"
     gene            2199..3635
                     /locus_tag="PK614_07325"
     CDS             2199..3635
                     /locus_tag="PK614_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRB82060.1"
                     /translation="MRRLALVLSSTLLTACAVGPDFTKPDATTPDAFRMAEAGGNAAS
                     IANTPWWELLKDQDLQKLIRTALEENKDLQRAAAAVEEFQARLFIAKTDFAPQMNVTS
                     NAPLFGRKTNFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREESRRA
                     IVLQLVSGVAEAYFELLQFDMQLDIARRTLKSWEESVRIAQARLRQGMISKLDADQFE
                     AERANAAAKAAEFERQKVQKENQLSVLLGRNPGRIARGHSLTEQVMPPDVPPGLPSEL
                     LQRRPDILQAEQDLAAATARIGMAKADRFPKLSITGILGVASPHLSRLVANETAFGVA
                     GPGFAGPLLNAQILGFQQKAAEAQARQAVAQYEQSVLVAFKEVEDSLVAVSTVREQRT
                     AQLQQVDALRSALSLANLRYKGGLANYLDVLIAQRNLFEAELALMGTHRLHLVSIVQL
                     YKALGGGWTPEGQPPVQALPVVSTQETR"
BASE COUNT          703 a         1034 c         1198 g          918 t
ORIGIN      
        1 cgatattcct tacgacacga cacgcttcat cgatgtctcc atcaaagaag tggtgaagac
       61 gctggccgaa gcgatggtgc tggtcattct ggtggtctat ctcttcctcc agagttggcg
      121 cgcgaccttg attcccggcg tggcggtgcc ggtctccctg gtcggcacct ttgccgggat
      181 gcaggccttg ggtttctcga tcaatacctt gaccctgttc ggcatggtgc tggcgatcgg
      241 catcgtggtg gatgatgcga ttgtcgtcgt ggaaaattgc gaacgacaca tgacccaggg
      301 ggggctttct gccaagaatg ccgccaagcg ggcgatggaa gaggtgacgg ggccggtcat
      361 tgcgattgtg ttggtgttgt gcgcagtgtt tgtgccggtc ggattcttgg gtggcattac
      421 cggcgagttg tataagcagt tcgcgattac gatttcgatc gcagtcatta tctccgggtt
      481 tgtcgcgttg accctcagtc ccgcgctctg tgctctcgtt ctgaagcccg gtgaggagca
      541 gcaccatgga atttttggcc tcttcaatcg attcttttcg tggatgcagt tgcggtatat
      601 ctcgacggtc ggccttgcgc tgactcgacg tgtcttgtcg gtggcggtgt tcgcagtcct
      661 gttgatcggt gtgttcatgt tgttcagggt cattccgagc agtttcctcc cggaggaaga
      721 ccagggctac ttcatcacca tcgtgcagct gcccgatggc gcctccaagc agcggacgga
      781 tgcagtactg agcaagatcg agagctattt cctggccagc ccggtcatcc attcaaccga
      841 tgccctgtcc ggtcagaact tcgtcttcaa tacgcggggg ccgaatgccg ccacgatgtt
      901 cgtgccgttg aaacattggg acgagcgcgc ggctccgcag catcatgtga aagcgctcat
      961 cggcgccgcc tatggagagt tcgccaagat cccggaagca ctcattctgg ctttcaatgc
     1021 gccttctatt cggggacttg gggctacggg tgggtttacc ctgcagcttc aggatccgag
     1081 cagcggggat ttcaacaagt tctccgccgc cgctcaggag ttcgtcggta aggcgcggca
     1141 gaatccggcg atcggcgcga tcggtacgag ttttcgcgtc agtgcgccgc gcattcttgc
     1201 gacggtgaat cgcgagcggg cgaaggcgct gggtgtgccg atctcagaag tgttcgatac
     1261 gatgcaggcg tatttcggta atttctacat caacgacttc atcaagttcg gtcgcgtctt
     1321 ccgggtgcag accgaggccg atgcgcagta tcgatccacg ccggaggata tttcgaagat
     1381 ttatgtgcgg gcgagtggcc ctcagggtac caccatgatt ccattggaca ccgtggtgag
     1441 cacggagttc acgagcggtc ccgatcctgt gacccacttc aatggctaca ataccgcgct
     1501 ggtgttgggg gcggcagccc ctggattcag ttcaggccag gtgctggatg cgctcgaaca
     1561 agtggcgaaa gaggtgctgg ttccgcaggg atacgggatc gattggagcg gcatttccta
     1621 tcaggagcgt atggtcggca gtcagtcgct ctatgcattc gcattcgggt tgctgatggt
     1681 gttcctggtg ctggctgccc agtatgaaag ctgggtggtg ccgtttgcgg tgattctggc
     1741 ggtgccgatc ggtctcttcg gcgcgctcag cgccgtttgg ctcaagggca tgaccaacga
     1801 tatctacttc cagatcggac tcgtgacact gatcggactc tcggcgaaaa acgcgattct
     1861 catcgtggaa tttgcgaaca aacggttcga ggaaggccat ccgttgctgg aagcgacgat
     1921 cgaggcggcg aagctccggt ttcgtccgat tgtcatgacg tcgatggcat tcattctcgg
     1981 cgtggtacct ctggtcattg ccacgggcgc cggcgctgcc agccgaaact ccatcggcac
     2041 cggggtgttc ggcgggatgc tggccgccac ctttctggct atctttttcg tgccgttatt
     2101 ttttgtgctg atccgctcgt tgagtcgccg cttgaaaaag ccgactcctc cggcacagga
     2161 tgcaccggac gatcccgaaa aggagcgcga ctatcaacat gcgtagactg gccctggttc
     2221 tttcctcgac ccttctgacg gcttgtgctg ttggcccaga cttcaccaaa cctgacgcga
     2281 cgactccgga tgccttccgc atggcggagg cgggtgggaa tgctgcatca atcgcgaata
     2341 caccctggtg ggagctgctc aaggatcaag atctccaaaa gctgattcgt acggcattgg
     2401 aagaaaataa ggacctccag cgtgcggcgg cggcggtgga ggagttccaa gcccgcctgt
     2461 ttatcgctaa aacggatttt gccccgcaga tgaatgtcac gtcgaatgcg ccgctgttcg
     2521 gtcggaagac caatttcttg tttcccggct tccccaatcc cttcaactac tacctgcagg
     2581 gaaatttgtc ctgggagatc gacatctggg gacgtattcg tcgatccaat gaggccgcgc
     2641 ggggcgatct gttgtcacgg gaggagagtc ggcgcgcgat tgtgttgcag ttggtgagtg
     2701 gtgtcgcgga ggcctacttt gagctgttgc agttcgatat gcaactcgac attgcgcggc
     2761 gtaccctcaa gtcctgggag gagtccgtca ggatcgctca ggcgcgattg cggcagggaa
     2821 tgatttccaa gctcgatgcc gatcaattcg aggccgaacg ggctaatgct gcggctaagg
     2881 cggcggagtt cgaacgtcag aaggtacaga aggaaaatca actcagtgtg ctgctgggac
     2941 ggaatccagg ccggattgcc cgcgggcatt ccttgacgga gcaagtgatg ccaccggacg
     3001 tgcctcccgg ccttccttct gaattgttgc agcggcgtcc tgacattctg caggcggagc
     3061 aggatcttgc cgccgccacg gctcgcatcg ggatggcgaa agctgatcgc tttccgaagc
     3121 tcagtatcac cggcatcctg ggtgtggcga gtccgcattt gtcccgttta gtggccaatg
     3181 aaaccgcgtt cggtgtcgcg gggccaggct tcgccggacc attgttgaat gctcagatcc
     3241 tcggtttcca acagaaagct gccgaggcgc aagcgcgaca agccgtggct caatatgagc
     3301 agtcggtgtt ggtcgcgttc aaggaggtgg aggattcgct ggtcgcagtc agcacggttc
     3361 gtgaacagcg cacggcacag ttgcagcagg tcgacgcgtt gcggtctgcg ctgagtcttg
     3421 ccaatcttcg ttacaagggt ggcctggcaa attacctgga tgtgttgatt gcgcagcgca
     3481 atctttttga ggctgagttg gcgctgatgg gaacccatcg gctacatctc gtctccattg
     3541 tgcaattata taaggcgctt ggcggtggct ggaccccgga agggcagccg ccggttcagg
     3601 ccctgccggt ggtctctaca caggaaacgc gatagtgacg ttgtctcttg gggcgtaggt
     3661 ctagtctgtt gctatgccct gatacttgtt cgctcgcccg ctttctcgtc gatctcccat
     3721 aagctaagcc gcagcccctt cacctcgctt caacctgagc gctcccggat ttttcgggtt
     3781 gtgcttccgt tacctgaaag acagagaggg ctccagaaat tcggacagtg tgttaagtgg
     3841 tagcctggct tca
//
LOCUS       DAORQN010000134         3786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_858_length_3786_cov_4.585098, whole genome shotgun sequence.
ACCESSION   DAORQN010000134 DAORQN010000000
VERSION     DAORQN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_3786_cov_4.585098"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK614_07330"
     CDS             complement(<1..215)
                     /locus_tag="PK614_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82061.1"
                     /translation="MMLAWYHKQKSVSMLRSVLVAANRANRILSVAGLTMLLLTSPAA
                     AQSVSSVRGLGGGVAPLFGLVGPGNLY"
     gene            264..1814
                     /locus_tag="PK614_07335"
     CDS             264..1814
                     /locus_tag="PK614_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB82062.1"
                     /translation="MTNSILRRCFFIGSAAALLLWTAPDSFGLEVNKPVPTERREAMS
                     LADAVLKALQNNLDISIGRQTRESRLADIVIEQAKFDPTVSLNGQYNRQVSPLNRPIL
                     GFTGANLQEITKFDQNSHSLTADITQNLPTGANYDLNYSPQRSYVSGPNTFLFNPAWT
                     GGLALTVTQPLLKNFGTDINKTFISIAQNNATVEQHVFLDRVLTVIASVEQTFWEMVF
                     ANENLKVAQAALKAAEELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAEKSIR
                     DQEDQLRRLLNPVEEELRQDLRLIPTDPPVTSLEAISLQEAIDIAMERRPEVLQAGKN
                     VESSDLNVKFAKNQLLPTLSVQGTMGLSGLGADYGDATRRNLGGDFYNYGAGLVLSYP
                     IGNRSAYSTYNKRQLESRNAQSSLQSVRQQVIVGVREAVRRVHTDFKRIETTRSARIM
                     AEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAVLDYNKSLSNLARNKATTL
                     ERYSLRLD"
     gene            1786..>2376
                     /locus_tag="PK614_07340"
     CDS             1786..>2376
                     /locus_tag="PK614_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82063.1"
                     /translation="MSDTVCGSTNLSMIKPDDASSTHATQAGERGAALVLLPLTATIL
                     FYSSPTTLQQHSLVQFLPQACAYAAFIAWSTINGSVARRIGLARPLVPQGLRWGLLTG
                     LTLGLINVLVILYLVPILGGDYRFLADTPHAKIPLLIMVPWFILLIATMVELNFRGFL
                     LGRLLTLGLPAPIAVPASALLFAFDPFLVATFQHLHW"
     assembly_gap    2377..2403
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2609..3628
                     /locus_tag="PK614_07345"
     CDS             2609..3628
                     /locus_tag="PK614_07345"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82064.1"
                     /translation="MNPSFSGHLVNDVFGDPGLFVEVRWSKRALLFDLGHNDALGPTP
                     LLRAGDIFISHTHMDHFIGFDALLRVALGRGKTLHLYGPPGLIANVQGKLHGYTWNLV
                     DGYPLTITVQEFHEQTIQRATFLATDGFQRHDAPEGAPLSRQVGHRFSVLADPMFTVE
                     AVALNHRIPSLAYSLQEQFHVNVNKERLHEAGLPVGYWLKEVKQYLWQGKPDDFRFQA
                     TLYHEHHKEERELVLGEVRERFITISRGQKLAYVVDARYDEENETKIVELAQGADIFY
                     CEAPYLDQDADKARDRYHLTARQAGVMAKKAGVRELVVFHFSPRYTGLGHRIEAEAQQ
                     AFRGS"
BASE COUNT          787 a         1233 c         1011 g          728 t
ORIGIN      
        1 atatagaggt ttcccggccc caccagacca aacagcggcg caacccctcc ccccagacct
       61 cgtacactcg agacggattg cgccgctgcc ggggaagtca gcagcagcat cgtcagacca
      121 gccaccgaca ggatcctgtt cgcgcgattc gcagccacca acacgctcct cagcatggac
      181 accgacttct gtttatggta ccacgccagc atcattttga attcagtatg gcccaacgca
      241 gacctcgcat caaggagttt gttatgacga attcaatcct ccggaggtgc tttttcatcg
      301 ggagcgcagc ggcgctcctc ctctggacgg cacccgattc cttcggactg gaagtgaaca
      361 agccggtccc gacggaacgc cgggaggcca tgtcgctcgc cgacgccgtc ctgaaggcct
      421 tgcagaacaa tctcgatatc agtatcggtc gccaaacgag agagagccgc ctggccgaca
      481 tcgtgatcga gcaggcgaag ttcgatccga ccgtcagttt gaacgggcaa tacaaccgtc
      541 aggtctcacc gctcaaccgg ccgatcctgg gattcaccgg ggcaaacctg caggagatca
      601 ccaaattcga ccagaactcc cactcgctca ccgccgacat cacacagaat cttccgaccg
      661 gcgccaacta cgatttgaac tacagtcccc aacgcagcta cgtgagcggc cccaacacct
      721 tcctcttcaa cccggcctgg accggcggac tcgccttgac ggtcacccaa ccgttgttga
      781 agaacttcgg aaccgacatc aacaagacgt tcatctcgat cgcgcagaac aatgccacgg
      841 tcgagcaaca tgtctttctc gaccgtgtgc tcaccgtcat tgccagcgtt gaacagacat
      901 tttgggagat ggtgttcgcg aatgaaaatc tcaaagtcgc gcaggcggcg ctcaaggcag
      961 cggaagaact gctggccagc aaccgcgcca aagccaaggc cggggtcatg tcgatcgtgg
     1021 atgtgctgca ggccgaagcc gccgtcgcct cgcgagtcga gcagatcctc gtcgcggaaa
     1081 aatcgattcg cgatcaggag gaccagttgc gccgcctcct gaaccccgtc gaggaggaac
     1141 tccggcagga cttgcgcctg atccccaccg atccacctgt cacatcactg gaggcgatca
     1201 gcttgcaaga agccatcgat atcgccatgg agcgccggcc ggaggtgtta caagcaggga
     1261 agaatgtgga gagcagcgac ctgaatgtga agttcgccaa gaaccagctc ctcccgaccc
     1321 tgtccgtaca gggcacgatg ggactgtcgg ggctcggcgc agattacggc gacgccacgc
     1381 ggcggaactt gggtggtgac ttctacaatt atggtgccgg ccttgtcctc agttacccca
     1441 ttggaaaccg gtcggcgtac agcacctata acaaacggca attggaatcc cgtaacgccc
     1501 agtcgtcgct ccagagcgtc cgtcagcagg tgatcgtcgg cgtccgggaa gcggtccgtc
     1561 gcgtccacac cgatttcaag cgcatcgaaa ccacccgctc cgcccgtatc atggccgaga
     1621 aacaactaca ggccgaacag gaacggctga aggtcggtct cagtacgacg cgcttcgtgc
     1681 tcgactttca gcgcgacctg gccaccgccc aaggcaacga gcttcgagcg gtgttggatt
     1741 acaacaagtc gctgtcgaat ctggcccgta acaaggccac cacccttgag cgatacagtc
     1801 tgcggctcga ctaacctgtc catgatcaag cctgacgacg cgtcctccac tcatgcgact
     1861 caggccggcg aacggggagc agccctcgtg ctgctccccc tgactgccac aatcctgttt
     1921 tacagctcac cgaccacact ccaacaacac agcctcgttc agtttctccc ccaggcctgt
     1981 gcctatgccg ccttcattgc ctggagcacg atcaatggat ccgtcgcccg tcgaatcggg
     2041 ctagctcgcc cgcttgtccc gcagggcctc cgttggggcc tgctcaccgg cctgacactc
     2101 ggactgatca atgtgctggt catcctctac ctcgttccaa ttcttggggg agactacaga
     2161 tttctcgccg ataccccgca cgccaaaatt cccctgctga tcatggtgcc ctggttcatc
     2221 ctgctcatcg ccaccatggt cgagttgaac tttcgcggct ttctcctggg gcggctcctc
     2281 accctggggc tcccggctcc gattgccgtg ccggcgagcg ccctgctgtt tgcgttcgat
     2341 ccctttctgg tggcaacctt tcaacatctg cattggnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnncggcttg gtgtggggta ccctatgggt catgctcaga aacctctacg cgccgatcgt
     2461 ggcccacgcc gtcgaagtca tcatcctcta cagcgtcatg cgagcgatat tgacctgata
     2521 ttgccctgtt ccgacagccg ggagaaccta aagtaaccgt tgcattcacg atggattcac
     2581 cgctcctgac aaagcaatac agtactccat gaacccttcc ttctcaggtc acctcgtcaa
     2641 tgacgtcttc ggcgatcccg gtctgttcgt ggaggttcgt tggtccaaac gcgctctcct
     2701 cttcgatctg ggccacaacg atgccctggg cccgaccccc ctcctgcggg ccggcgacat
     2761 cttcatttcg cacacccaca tggaccactt tatcggcttc gatgcgctgc tccgggtcgc
     2821 ccttggacgc gggaaaacac tccacctcta cgggccgccc ggcctcatcg ccaatgttca
     2881 aggaaaactt cacggctaca cctggaacct cgtggatggg tatccgctca ccatcaccgt
     2941 gcaggaattc cacgagcaga caatccagcg cgccaccttc ctggcaacgg acggattcca
     3001 gcgccacgac gctccggagg gagcccccct cagccggcaa gtcggccatc gtttttccgt
     3061 gctcgcagat cccatgttca cggtcgaagc ggtcgcactc aatcaccgca ttccttccct
     3121 ggcctactcg ctgcaggaac agtttcatgt gaacgtcaac aaggagcggc tgcacgaagc
     3181 cgggctgccg gtgggctatt ggctgaagga agtgaagcag tacctctggc agggcaagcc
     3241 ggacgacttt cggtttcagg cgacgctcta ccatgaacat cacaaggagg aacgcgagct
     3301 tgtcctgggt gaggtccgcg agcgattcat caccatcagc cgcggccaaa aacttgccta
     3361 tgtcgtggac gcccgctacg acgaagaaaa tgaaaccaag atcgtcgagc ttgcgcaagg
     3421 cgccgacatc ttttactgtg aagcccccta cctggatcag gatgcggaca aggcaaggga
     3481 tcgttaccac ctcaccgcgc gccaggccgg agtcatggcg aagaaagccg gtgtgcgcga
     3541 gctggtcgtg ttccacttct ccccgcgcta cacgggacta ggccaccgga tcgaggcgga
     3601 agcgcaacag gcatttcgag gcagctgaag gaggtcgcgg atgggcgagt gggcgaagta
     3661 cggattgtac ttcttgttgg gcggcaccat tgtcagcatc tcaacctatc tgggatcaca
     3721 aggccggtcg tttctcgccg ccttcgccag cacctttccc gcgatgaccg gcgcgacgtt
     3781 catcct
//
LOCUS       DAORQN010000135         3775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_862_length_3775_cov_8.090323, whole genome shotgun sequence.
ACCESSION   DAORQN010000135 DAORQN010000000
VERSION     DAORQN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_862_length_3775_cov_8.090323"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..187)
                     /locus_tag="PK614_07350"
     tRNA            complement(113..187)
                     /locus_tag="PK614_07350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Gly,seq:tcc)
     gene            complement(331..416)
                     /locus_tag="PK614_07355"
     tRNA            complement(331..416)
                     /locus_tag="PK614_07355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(380..382),aa:Tyr,seq:gta)
     gene            complement(654..1589)
                     /locus_tag="PK614_07360"
     CDS             complement(654..1589)
                     /locus_tag="PK614_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82065.1"
                     /translation="MRPVLVYNGWFRAVLPTMAAGLVLGLLIFGQASAETAAPSSDSS
                     RLWGALMAEAKALHLPTQFLEQIPAQFVTFEFEDLHAFAAEYHPVEHRMVLDRSLSLN
                     AAGRTLRPLARLTHKELETLYHELFHAYMDFLEQERAPSAAHASFMAFAREQQRCRYQ
                     QVFITPVLQKKDLKEERFLSEAESWEALNETWAVFVGWAIWTHLEVERKGARELRSSG
                     RSLNVPAWIARLSQAEQDASLRGYYEPEDPGEKAMARKRFLAPDFRLSAPELLLLMKE
                     VLGSPADMTRQADQVLTRGKVVPASRGACEAPAAS"
     gene            complement(1656..3767)
                     /locus_tag="PK614_07365"
     CDS             complement(1656..3767)
                     /locus_tag="PK614_07365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRB82066.1"
                     /translation="MCRCKVSGGMLYSATAPVMSLTAQSDANMDRESKPYILKRTPDQ
                     DHVRKFSLDYAKELNAQQYAAVTAADGPALVIAGAGSGKTRTLVHRVAYLIDSGVDPS
                     HILLLTFTRKSSEEMLERVGALIGSRSQRVCGGTFHSVANMLLRRHGRVLGIEPGFTI
                     MDRGDAEDLIALLRAQLGLNEKDKRFPRKGTIAEIYSKCENTLRGLEEIVLDEFSHFA
                     DHLEALGKLQRAYQAAKRQRQLLDYDDLLVLLRDLLTKDEPTRRTISQQFRYILVDEY
                     QDTNRLQAELIRKLAATHDNVMVVGDDSQSIYAFRGATFRNIMEFPSWFPGAKIYKLE
                     ENYRSTQPILSLANEIIQEAPEKYTKHLFTRKLDGPLPALVEAAGENAQSRFVAQKIL
                     ELREEGVPLSEIAVLFRSSFHSFDLEIELSRCGLPFVKRGGIKFIEAAHVKDLLAHLR
                     VVVNPQDAVSWHRVLMLVEGVGPKKAHDLVAAMVRVNDPYQVLRDSSGRSGKGLKELA
                     LVLDSLSKSDDLSPTEQVNRVYEYYLPILKDHHDDYPKRIRDLDHLHTIAESYPSLTE
                     FLADLALAPPDGSAVGVEPAGRDDEQVVLSTIHSAKGLEWQCVFLLWVVDGKFPSVFS
                     FNTDEELEEERRLLYVAVTRAKRYLFLTYPINVYDRSSGMLLSKPSRFLDHVSPRLFE
                     TLALVEEGHGHEWGHEHDRYV"
BASE COUNT          856 a         1187 c          981 g          751 t
ORIGIN      
        1 ctcgtcctta ccaaggacgt gctctgccaa ctgagctacg tgggccctga actcacagtc
       61 ttggtccgcc ctcacacgct ctgtccgcac ccgcggtcga gcacgctgga tttggagcgg
      121 gcgatgggat ttgaacccac gacagccagc ttggaaggct ggaactctac cactgagcta
      181 cgcccgccca cggtgacccg accaacactc caacgatccg acggcgacac gctcggccac
      241 acaatgctgc gcaccgccag atcgcgctca ttatcattgc ccaaccggac gcacacgttt
      301 cacgtgagat tcggtcgaat acgctcagtt tggtgggcag gcaaggattc gaaccttgga
      361 agccataagg cagcagattt acagtctgcc ccctttgtcc acttgggtac ctgcccgcta
      421 ggcacacgtt cccaaactca agcgaatatt atttatttca aataaatccc gaaccagagc
      481 cacttcattc cctgaagaga agaatgaaag taagccccac catgcacagc tccccagcaa
      541 aacaacaggg ctggagcgct gatgaaatga ggattgacct ggcttcgaaa gcagccgatt
      601 ttatttgcga cgcgttccat tgtcaagggg gtatgttcta tgcaggaaca aatctacgat
      661 gcggcaggag cctcacaggc tccacgggag gcaggaacaa ccttcccgcg cgtcaatacc
      721 tgatcagcct ggcgagtcat gtccgctgga ctacccaaaa cttccttcat gagtaacaag
      781 agttccggag cagacaggcg aaagtccggc gccagaaatc gcttgcgtgc catcgccttt
      841 tcacctggat cctccggctc gtaatatcct ctcaggcttg cgtcctgctc cgcctgactg
      901 agccgggcaa tccaagccgg aacgttcagc gaccgaccgg atgatcgaag ttcacgcgcc
      961 cccttcctct ccacttccag gtgggtccag atcgcccatc ccacaaacac cgcccaggtt
     1021 tcattcaacg cttcccacga ctccgcctcg ctgagaaagc gctcctcttt caaatctttc
     1081 ttttggagca ccggcgtgat gaagacctgc tgatagcgac accgttgttg ctcgcgggcg
     1141 aatgccataa acgacgcgtg ggcggctgac ggcgcacgct cctgctcgag aaaatccatg
     1201 taggcatgaa acagttcgtg atagagcgtt tccaactcct tgtgcgtcaa ccgcgccaat
     1261 ggacgcagag tcctccccgc tgcattcaga gagagcgatc gatccaacac cattcgatgc
     1321 tcgaccgggt gatactccgc ggcaaacgcg tgcagatcct caaactcaaa cgttacaaac
     1381 tgcgctggaa tttgttcgag gaactgagtg ggaagatgga gggctttcgc ttccgccatc
     1441 aatgcgcccc agaggcgcga agagtccgaa gatggcgcgg ccgtctctgc gctggcctgg
     1501 ccgaagatca gaagtcccag caccagcccc gcggccatcg tgggaagtac agctcggaac
     1561 caaccgttat agacgagcac cggacgcatg atgatcttga atcgtagaat ggagtacggc
     1621 aacccaacca gagttatacg gtgggaacga tcgacctaga cgtaccggtc atgctcgtgc
     1681 ccccactcat gcccatggcc ttcttcgacc agagccaacg tttcaaacag acggggcgac
     1741 acgtgatcca ggaatcgcga tggcttggat aacaacatgc cggaactccg gtcatacaca
     1801 ttgatcggat aggtcaaaaa caggtagcgc ttcgcccgag tcaccgcgac atacaataac
     1861 cgccgctcct cttccaactc ttcatccgta ttgaatgaga agaccgaggg gaatttccca
     1921 tccaccaccc ataacaaaaa tacgcactgc cactccagcc ccttcgccga atgaatcgtc
     1981 gacagcacca cctgctcgtc gtcgcgcccc gcaggttcca cacccaccgc actgccatcc
     2041 ggcggggcca gcgctaaatc agcgaggaac tctgtcaggc tggggtaact ctctgcgatg
     2101 gtatgcaaat ggtccagatc ccggatccgc ttgggatagt catcatgatg atccttcagg
     2161 atcggcagat aatactcata gactcggttg acctgctcgg tcgggctcag atcatcactc
     2221 ttcgacaggc tgtcgagcac cagcgccagc tccttcaacc ccttgcccga gcggccgcta
     2281 ctgtcccgca gcacctgata cggatcgttc accctcacca tggccgccac cagatcatgc
     2341 gccttcttgg gaccgacccc ttcgaccagc atgagcacgc gatgccaact gaccgcatcc
     2401 tgaggattca cgaccacccg caaatgggcc aggagatcct tcacatgcgc cgcctcaatg
     2461 aacttgattc ctccccgctt cacgaacggc aagccgcagc gcgacagctc aatttccaga
     2521 tcgaaggagt gaaagctgga gcgaaagagc acggcaatct cactgagcgg cacgccttct
     2581 tcgcgcagtt ccaggatttt ttgcgccaca aatcgagact gcgcattctc cccggccgcc
     2641 tccaccagcg ccggcagcgg cccgtccaac ttacgcgtga acagatgttt cgtatatttc
     2701 tcaggggctt cctgaatgat ctcgttggcc aggctgagaa tcggctgcgt gctgcggtaa
     2761 ttttcctcca gcttgtagat cttggcgccg gggaaccagg agggaaactc catgatgttg
     2821 cggaacgtcg caccgcggaa cgcatagatg gactgtgaat cgtcacccac gaccatgaca
     2881 ttgtcgtgag tagccgctag ctttcgaatg agttccgcct gcagccgatt cgtgtcctga
     2941 tactcatcca ccaggatgta gcggaattgt tgcgagatgg tccgccgggt gggttcgtcc
     3001 ttggtcaaca agtcccgcaa cagcaccagg agatcgtcat aatcaagcaa ctggcgctgg
     3061 cgcttcgcag cctgataggc gcgctggagc ttccccagcg cctccagatg gtccgcaaag
     3121 tgggaaaact cgtcgaggac aatctcttcc aatcctcgca acgtgttctc gcacttgctg
     3181 taaatctccg cgatggttcc cttgcgaggg aaccgcttat ctttttcgtt caggcccaat
     3241 tgggcgcgca agagggcgat cagatcttcg gcatcgccgc gatccataat cgtaaagccc
     3301 ggttcgatcc cgaggactcg tccatggcgc cgcagcagca tgttggcgac cgaatggaag
     3361 gtgccgccac aaactcgctg gctacgcgat ccgatcaaag cgccgacacg ctccagcatc
     3421 tcctccgaag acttcctcgt gaaggtcagc agcaaaatgt gcgagggatc caccccggaa
     3481 tcgatcagat aggcgacgcg atgcacgagc gtccgcgttt tgccgcttcc ggcaccggca
     3541 atgaccagcg cagggccatc ggccgccgtc acggccgcat actgctgcgc gttcaactcc
     3601 ttggcataat ccaacgagaa cttccggacg tggtcttgat ccggagtccg tttcagaatg
     3661 tagggcttac tctctcggtc catgttggca tccgactggg cagtgaggct catgacggga
     3721 gcggtcgcgc tgtataacat accaccggag accttgcaac gacacagttc gtgag
//
LOCUS       DAORQN010000136         3757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_867_length_3757_cov_6.457861, whole genome shotgun sequence.
ACCESSION   DAORQN010000136 DAORQN010000000
VERSION     DAORQN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_3757_cov_6.457861"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>730
                     /locus_tag="PK614_07370"
     CDS             <1..>730
                     /locus_tag="PK614_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB82067.1"
                     /translation="MGQGNFRATMDGQAPRELRELGDTVKWMGARLQQLDDIKSEFLA
                     HVSHELRTPMASIQEGTHLLLDEIPGPLTQDQRTTLRIMSDSSRRLMYLISTILDLSK
                     MEAGMMEYRFAPTDLRRIADVSVNKIRLLADAKQVRFLVEAPVERHWVRADSARIEQV
                     LDNLLSNALKYSPEGAAVKLHMEPRRDEGLLFVDVTDCGPGIPTEEVPHIFERFYQGR
                     TKTRPLSVGSGLGLALAKKVVEAHG"
     assembly_gap    731..740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1229..1594
                     /locus_tag="PK614_07375"
     CDS             1229..1594
                     /locus_tag="PK614_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82068.1"
                     /translation="MAQAIERTVHDLLDRELTIQQLRALQDSEAQSLEALQRELQDQE
                     RKAELFNSRREHPRMSVEPATLQSLQRQVSDRDRKIEELTSQLEALKRIDQEMRGKTR
                     PIKPPSNVFSPQQPESSKP"
     gene            1603..3018
                     /locus_tag="PK614_07380"
     CDS             1603..3018
                     /locus_tag="PK614_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB82069.1"
                     /translation="MEQERILVVDDDEGLLHLLRMRLSALGFSVTPCTGGRDALTAAR
                     QETFDIAITDLRLRAEDGLALTEELMQIQPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKATLDKALIQLRMTREIQRLKSLVKELYGLENVIARSPAMQRLFQQVAQ
                     IADSDATILLTGETGTGKEVLARVLHANSRRSKGPFVALNCAAISETLFESELFGHIR
                     GAFTNAVAAKRGLFQSANGGTLFLDEIAEMSLPMQVKLLRAVQEREVREVGADYATKV
                     DVRIITATNKDLAESVKAGTFRHDLYYRVSVVPLAIPPLRERKDDIPLLAQHFLKQSA
                     KRSNKDVRGFTPAAMHRLMVYPWPGNVRELENAVEKAVVMSRQDMVLPELLPTAGVAS
                     DIGLKPLTEAKEEFERSYLRNVLQMTGGNISRAAQFAGRYRADFYKMLKKYGLHPSML
                     KERADLDLTDLEEATEEVKDV"
     gene            complement(3015..3317)
                     /locus_tag="PK614_07385"
     CDS             complement(3015..3317)
                     /locus_tag="PK614_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB82070.1"
                     /translation="MICGTGYYLSEDFLGKGTLWDMSPGGWRIQGDHQVKIGMRLTLR
                     MELPEEKMPLEIEQAVVQWVRGKDFGVRIKKIRRSAAIKLERLVGYHLCILPPVEH"
     gene            complement(3394..>3757)
                     /locus_tag="PK614_07390"
     CDS             complement(3394..>3757)
                     /locus_tag="PK614_07390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB82071.1"
                     /translation="SQRVSVDCSVMFAGENVVAEGRILDLSLPGCLMESSKKMSAGEY
                     IQLRLFLPDHQAPLHVALAAIRWAEGSKVGIEFIRTSQPEQHRLEQFVRRSSGCDASS
                     TLSDGGVLAGTAWNEKQA"
BASE COUNT          880 a          937 c         1083 g          847 t
ORIGIN      
        1 aaatggggca ggggaatttc cgggcgacaa tggatggtca ggcaccccgt gaacttcgag
       61 aattgggtga tacggtgaag tggatggggg ccaggctgca gcaactcgac gatatcaaga
      121 gcgagtttct tgcccatgtg tcccatgaac tgcgtacgcc gatggcttcg attcaggagg
      181 ggacccatct tctcttggat gaaattcccg gcccgctgac gcaggaccag cggacgacat
      241 tacggatcat gtccgacagt agccggcggt tgatgtacct catttcgacc attctcgact
      301 tgtcgaaaat ggaggccggc atgatggagt accggtttgc tccgaccgac ctgaggcgga
      361 tcgctgacgt ttcagtcaat aaaatcaggt tgttggcgga tgcgaaacaa gtgcgattcc
      421 tggtcgaagc tccggtcgaa cgccattggg tgagggctga tagcgctcgt atcgaacaag
      481 tgctggacaa tctgctctcg aatgcgttga agtacagccc ggagggggcg gccgtgaagt
      541 tgcacatgga gccgcggcgt gatgaagggt tgctgttcgt cgatgtgacg gactgtgggc
      601 cgggtatacc gacggaggaa gtcccgcata ttttcgagcg cttttatcag ggccgcacca
      661 agacacgacc actctcggtt ggcagtggac tggggcttgc gctggccaaa aaggtggtgg
      721 aagcccatgg nnnnnnnnnn cgtatctggg tcgaaagtaa agtcggaaaa ggaacgactg
      781 tgcggttcat tttgtgtctg acaaaaccgg ggatactggc atagtgtggc atctgcgaaa
      841 catggcagta ctgctgggac tcggcctctt gtcagcctgc agcactgtgt ctccgccccc
      901 cggccagaca ccctattttt atggtgatgg cagcgacgga aaggcgttgc aggccattat
      961 acgtaaagaa atccagttag tgccaaaatg tgctagtaaa aatacctgcg atcatgtatt
     1021 cttcacgcgc gcctaggcgt ctctgtatga aagtcgggag tcagcaagcc agtattttga
     1081 gaaagttatt gctgtcgcgc cgaagagtca gctggcagcg tcgagtcgcc tctggctgca
     1141 attgttgcag tcagaagaag tgcctgccga tcagtcctgg atccgttccg tctggaatgc
     1201 tccggctact gcacattcac agaggctttt ggcccaggca attgagcgga ccgtacacga
     1261 cttgcttgat cgcgaactca caattcagca attacgggcg ctgcaggata gtgaggctca
     1321 gtcgctcgag gcgttgcaac gggaacttca agaccaggag cggaaggcgg aattgttcaa
     1381 ttccagaaga gaacacccgc ggatgtcggt cgagccggct acgctccaaa gcttgcaacg
     1441 acaagtcagt gatcgagata gaaaaattga ggagctcacg agtcagttag aagccctcaa
     1501 gcgcattgat caggaaatga ggggaaagac ccgcccgatc aaacctccgt cgaatgtttt
     1561 ttcgcctcaa cagccggagt cctccaaacc ctaggggccg tcatggagca ggaacgcatt
     1621 ctcgttgtag atgatgacga aggattgttg catctgttaa ggatgcggct gtcggcgctt
     1681 gggttttctg tgacgccgtg caccggtggt cgcgatgcgc tcacggctgc tcggcaggaa
     1741 acgtttgaca tcgctattac tgacttgagg ttgcgagctg aagatgggtt ggccttgacc
     1801 gaagaactca tgcagatcca gccgggcctg ccggtcatca ttcttacggc tcatgggagc
     1861 attcccaatg ccgtcgaggc gatgcaacgc ggggctttcg gctacctgac gaagccgttc
     1921 gatgataaag aactaaaggc gaccctcgac aaggcgctga ttcagttacg aatgacgcgt
     1981 gagattcagc gcctaaaatc tttagtcaag gagctatacg gactggagaa cgtgattgcc
     2041 agaagtcccg caatgcagcg attgttccag caagttgcgc agattgcgga ttcagatgcg
     2101 accattctgt tgaccggaga gaccggaaca gggaaagaag tgctcgcgcg cgtacttcat
     2161 gccaatagtc gtcgttccaa agggccattt gtggccttga attgtgcggc aatcagcgag
     2221 accctctttg aaagtgaatt attcggacat attcgcggcg ccttcaccaa tgcggtcgcg
     2281 gccaagcgag gtctctttca aagtgcgaat gggggcactc tctttctcga cgagattgcc
     2341 gaaatgtcct tacccatgca agtgaaactt ctacgtgcgg tacaggagcg agaagtgcgg
     2401 gaggtggggg cagactacgc aaccaaggta gacgtgcgca tcattacagc cacgaacaag
     2461 gatctggcag aaagcgtaaa agccggaacc ttccgtcatg acctctacta ccgagtatcc
     2521 gtagtccctc tagctatccc cccactgcgg gagcggaaag acgatattcc gcttcttgcg
     2581 cagcattttt tgaaacagag cgctaaacgt tcgaacaagg atgttcgtgg ctttactcct
     2641 gcagccatgc atcgactcat ggtgtatcca tggcctggca atgtgcgaga gctcgaaaat
     2701 gctgtcgaaa aggctgtcgt gatgtcccgg caagatatgg tattgccaga gttactccct
     2761 acggctggtg tggcgtcaga tatcggcttg aagccactaa ccgaggcgaa ggaagagttt
     2821 gaacgctcct atttgcgtaa tgtgctgcaa atgacgggcg ggaatatttc gcgggctgca
     2881 caattcgccg ggagatatcg ggcggatttc tacaagatgc tgaagaagta tgggttgcat
     2941 ccctccatgt tgaaggaacg cgcagatctt gatcttactg atctggaaga agcgacggaa
     3001 gaggttaagg atgtctagtg ctcgacagga ggaagtatgc acaagtggta gcccacaaga
     3061 cgctctagtt taatggccgc cgaccgacgg attttcttga tacggactcc aaagtccttt
     3121 cctctgaccc actgaacaac agcctgctcg atttccaacg gcattttctc ctcaggcagc
     3181 tccattcgca gtgtgagtcg catgccgatc ttgacttgat ggtctccttg gatacgccat
     3241 cctcccggtg acatatccca gagggtgcct ttccccagaa aatcttccga gagatagtat
     3301 cccgttccgc aaatcatggc tctctggtaa gtacggagtt tgaagtgttt cggcatggca
     3361 agtacttccc tggggatcaa tctgcggaaa cagctacgcc tgtttctcat tccacgctgt
     3421 tcctgcaagg acacccccat cgctcaatgt cgatgaagca tcgcatccgg aactccggcg
     3481 cacgaattgt tcaagtcgat gttgttcagg ttgggatgtg cggatgaact cgatgccaac
     3541 cttcgagcct tcggcccatc tgatggcggc gagtgcgaca tgtagcggag cctggtggtc
     3601 cggcagaaat aaccgaagtt ggatatattc accggcggac atctttttcg agctttccat
     3661 gaggcatcct gggagggaga ggtcgagaat ccgcccttcg gcaacaacgt tttccccggc
     3721 aaacatgacg gaacaatcta ctgatactcg ttggcta
//
LOCUS       DAORQN010000137         3679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_884_length_3679_cov_7.569812, whole genome shotgun sequence.
ACCESSION   DAORQN010000137 DAORQN010000000
VERSION     DAORQN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_3679_cov_7.569812"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK614_07395"
     CDS             complement(<1..445)
                     /locus_tag="PK614_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82072.1"
                     /translation="MKSRLLVLACMFLLSVVGLVYAEGHPEVFVQTGHLADAGSVAFS
                     SDGQWVLSGGSGTVKLWEVATGREVRTFTGISSYVRSVALSPSGEWALAGSWDGAVKL
                     WEVATGREVRSFMGHGYEVRSVAFSQDGRWALSGSGAGSVNMCEVA"
     gene            complement(521..1102)
                     /locus_tag="PK614_07400"
     CDS             complement(521..1102)
                     /locus_tag="PK614_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82073.1"
                     /translation="MAVLALLMTVGSGVATTKAVEPPATKLDCSVYETKSMNVSAAFT
                     VAQFVFNIIPSIGFSRTTGVAWDKVVHGTIARYVELCNRYNAGLVDKAEYEVRLKEIE
                     ALYKETKEMEEKLFAATRQRAKASGDEMDEALGRKRKDSSVPPEVEASVKALAEKVER
                     LDPIGKPLKPAAPCKPPDMLGAPGAQPDPGRNC"
     gene            complement(1175..2236)
                     /locus_tag="PK614_07405"
     CDS             complement(1175..2236)
                     /locus_tag="PK614_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRB82074.1"
                     /translation="MRGETDTNRYVVRYVLVCLSLVWAVVGPAPWPDAPARASTPAQI
                     YAAAAPAVVLVVASSGSDVMSGTGSIIDPTGLVLTNSHVIFDKDAKAPYRSMWVFLRP
                     DRVTGNEKTDLALRLQATLVAHDPELDLALLKVTGLSKALPVLPMGDPATIAIGSRVL
                     AIGHPEQGGLWSLTTGVISAEWENFTQVPGKHVFQTETGLNRGNSGGPLIDENGQQIG
                     VNTSMARKAKDGLAITSISFAVKSSVAKEWLAKQGVQVAYAKSFLPAGEGKDGGAPQV
                     GGTVPPIDSGPRQALPSAKPQPAKVLENQPEAKPETGPGLPPVRPFSLDRLMRGLAQV
                     SEDLESQMEQMQEEIRKRR"
     gene            complement(2288..2791)
                     /locus_tag="PK614_07410"
     CDS             complement(2288..2791)
                     /locus_tag="PK614_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82075.1"
                     /translation="MTIMNCECFLKAKLGAAGVVLSFLMICGTGAGATSPTDGWLITP
                     QEAASAPRAEAPGGALLDVGRQESNLGPTIEVLKPNDGGRASAPIEIQINFVPKTGAV
                     DLTSLKVTVVKLFPFDITDRVRDYATSAGIQMKEAKIPAGKHIVRISVADTDGARSVK
                     EIEFEVL"
     gene            complement(2991..3629)
                     /locus_tag="PK614_07415"
     CDS             complement(2991..3629)
                     /locus_tag="PK614_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HRB82076.1"
                     /translation="MKKMQAFLVTGTLAVATLVVGCAGQKPQQGGPLARTDTPIQEVK
                     DLPKWVTEKGAAFSGERRVLYGVGNSAGIYNPALRRKAAEGQSRNDLAATLQVYVAGL
                     QKQYMAETTAGSMEKSSVEQHIQDTFKQVTEATLVGAQVVQYWENPLRNEAYALARLD
                     IEQFMDIMKSYAAASGQFKELDANLREFVRKNADKAHDELNQELERRKHGAS"
BASE COUNT          672 a         1246 c          930 g          831 t
ORIGIN      
        1 tcgccacctc acacatattc accgacccgg ctccactccc tgaaagggcc caccgcccat
       61 cctgcgaaaa cgccacggac ctcacctcat atccatgccc catgaagctc cgtacctccc
      121 gccccgtcgc cacctcccag agcttcaccg ccccgtccca gctgcccgcg agcgcccatt
      181 ccccactcgg ggacaaggct acggatcgta cgtaagaact aatccccgtg aaggtccgca
      241 cctcccggcc cgtcgccacc tcccagagct tcaccgtccc gctcccgcca gaaagcaccc
      301 actggccatc cgaggaaaag gccacagagc ctgcatcagc aaggtgcccc gtctgcacaa
      361 acacctctgg atggccttct gcataaacca gtccgacgac gctgagtaga aacatgcatg
      421 ccaaaaccaa gagccgtgat ttcatagact tcatcctagc gaaggcccca cgggccgttg
      481 caccatctct actcggcgcc tctcctgccg gactccttac ttaacagttc cgccccggat
      541 ctggctgtgc gcccggcgct cctaacatat ccggcggctt acaaggcgca gccggcttga
      601 gcggtttgcc gatcgggtcg aggcgttcca ccttttcggc taaggccttg accgaggctt
      661 ctacttccgg cgggacagat gagtcttttc gctttctccc aagcgcttcg tccatctcat
      721 cccctgaggc cttggcgcgc tgccgtgtcg ccgcaaacag cttttcttcc atttccttgg
      781 tttctttata cagcgcttcg atctccttca atcgcacttc atattcggcc ttatcgacca
      841 aacccgcatt gtagcgatta cagagttcga catagcgtgc aatggtcccg tggacgacct
      901 tgtcccatgc aacccccgtc gtgcggctga acccgatcga cggaatgatg ttgaagacaa
      961 actgtgcgac ggtgaaggcc gctgacacgt tcatggattt ggtttcgtag acggaacaat
     1021 ctaacttggt agccggcggc tcgacggcct tggtggtcgc cacccctgag ccgaccgtca
     1081 tgagtagagc caagacggcc acgcagcagg cccggcctct ttctcgcatc catcttctca
     1141 ccgttcttgg tgtcacctgc ttcatgatcg ctccttaccg gcgcttacga atttcttcct
     1201 gcatctgttc catctgtgat tccagatctt cgctcacctg cgccaacccg cgcatgagcc
     1261 gatcaagact gaacggccgg accggcggca ggcctggtcc cgtttcgggt ttggcctccg
     1321 gttgattctc gagcaccttg gcaggctggg gcttggcgct cgggagtgcc tgtctggggc
     1381 cggaatcgat cggtgggaca gtacctccaa cttgcggcgc gccaccgtcc tttccttccc
     1441 cagcaggaag gaatgatttc gcataggcaa cctgcacgcc ctgcttggcc agccattcct
     1501 tggctacact ggacttcacg gcgaaactga tgctggtgat cgccaggccg tctttcgcct
     1561 ttctcgccat cgaggtgttg accccaatct gctgcccgtt ctcatcgatg agcggcccgc
     1621 cggagttgcc acgattcagg ccggtttctg tttgaaagac atgcttgcct gggacttggg
     1681 tgaagttctc ccactcagca ctgatcactc ccgtcgtcaa actccagagt ccgccctgct
     1741 cgggatgccc gatcgcgagc acgcgtgacc cgattgcaat cgtcgcggga tctcccatcg
     1801 gcagaactgg aagcgccttg gataggcctg tcactttcag caaagccaag tccaactcag
     1861 ggtcgtgcgc cacaagcgtc gcttggaggc gaagggcgag gtccgttttt tcatttcccg
     1921 tgactcgatc cgggcgcaag aagacccaca tgctgcggta gggagccttc gcatccttgt
     1981 cgaaaatcac atgtgagttc gtcaggacaa gaccggtcgg atcgatgatg gagccggtgc
     2041 cggacatgac atcgctccct gaactcgcta cgactaatac caccgccgga gcggccgccg
     2101 catagatctg agccggagtc gaagctcgtg ccggcgcatc aggccatggt gccggcccca
     2161 ccacggccca cacgagagat agacagacca agacgtatct gaccacatac cggttcgtgt
     2221 cggtttcgcc cctcatatcc ttctccattg tggcttcctc cccgcccccc gctcatcgac
     2281 ggcttattta caggacttcg aactcaatct ccttcacgct tctggcccca tccgtatccg
     2341 ccacactgat acgcacgatg tgcttgccgg ccgggatctt tgcttccttc atctgaatgc
     2401 ccgctgacgt ggcgtagtcg cgcacccggt ccgtgatgtc gaatggaaac aacttcacca
     2461 ccgtgacctt gagactggtc aaatccactg ctcctgtttt gggcacaaag ttgatctgaa
     2521 tttcgatcgg ggccgacgcc cggccgccgt cattgggctt caagacttcg atcgttggtc
     2581 cgagattgct ttcctgccgc cctacatcga gcagcgcccc acccggcgct tcagcccgcg
     2641 gagccgacgc cgcttcctgc ggggtgatga gccacccgtc tgtcggcgag gtggctccgg
     2701 ccccagtgcc gcagatcatc aggaatgaaa gaacaacccc tgccgcgccg agttttgctt
     2761 ttaggaaaca ctcacagttc atgatggtca tggtgcctcc tccttaaatt cactacagta
     2821 gcctcagcat gccagtgtgt ctgttcgaat ccgtgattcg cccgattggg agagacagac
     2881 tcacgccttc ctccgcatgg tcgcggcagg ctgcggaccc aagggggatg cttctcctac
     2941 gagggcccgc agcatccgcg agcgatcgcc gtccttctct acggcacctg ttagcttgcg
     3001 ccgtgttttc ttcgctccaa ctcctggttc agctcgtcgt gcgccttgtc ggcattcttc
     3061 ctcacaaact cgcgcaagtt ggcatccaat tctttgaact gtccgctggc cgcagcatag
     3121 cttttcatga tgtccataaa ttgttcgatg tcgagccgtg ccaaggcata ggcttcgttt
     3181 cgcagcggat tttcccaata ttgcacaacc tgcgcaccga ccaaggtcgc ttcggtcacc
     3241 tgtttgaacg tgtcttgaat atgctgctcc acgctcgact tctccatact gcccgctgtc
     3301 gtttcggcca tgtattgttt ctgaagcccc gcgacgtaga cctgcaaggt ggccgcaagg
     3361 tcgtttctgg attgaccctc cgccgccttc cgacgcaacg cagggttata gatgcccgca
     3421 ctgttgccga ctccgtacaa cacccgacgc tcgccgctga acgccgcccc cttctcggtg
     3481 acccacttcg gcagatcctt gacctcctga atcggcgtat ccgtcctggc cagagggcca
     3541 ccttgctgcg gcttttgtcc cgcacatccc acaaccaagg tcgccaccgc gagtgtccct
     3601 gttaccaaga atgcttgcat cttcttcatg tgctgcctcc tcctgtaaga atgattgtcc
     3661 ggttcccgac ttcctggta
//
LOCUS       DAORQN010000138         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_891_length_3652_cov_4.670559, whole genome shotgun sequence.
ACCESSION   DAORQN010000138 DAORQN010000000
VERSION     DAORQN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_3652_cov_4.670559"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PK614_07420"
     CDS             complement(<1..1094)
                     /locus_tag="PK614_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB82077.1"
                     /translation="MSLLTDVHDRIEQAREAPGCSSELHWTSFAEFFRSRLYDPRLVT
                     RNFLTYCDDDRNLRRTYSYAEFGTIVERMAEMFHAKFGLTRGDRIATVLFNHDLTALI
                     YFAAWTLGVAVVPINVEETPEKKRYILEHSEASVALCWQDVYEELKALQSMLPALREV
                     VALGDGGILDGKETRGKSRKNSHAVGARFAPGTSRLDDPALIIYTSGTTGPPKGVILT
                     VANLLIDADAIADRHGFGLTDRVMCVLPVHHVNGIVVTLMTPFYWKGSVVLNRKFKTG
                     SFWRRLHEEQVTSVSVVPTLLEFLLDADEDLAAYRLDHFGGVICGAGPLLKDTALRFE
                     ERFGFPIRHGYGLSETTCYSCFLPNDLTQE"
     gene            complement(1069..>1348)
                     /locus_tag="PK614_07425"
     CDS             complement(1069..>1348)
                     /locus_tag="PK614_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRB82078.1"
                     /translation="CGDHSWYDIASTLHRIMREEKGLHPNLDFYTAVAYLLMGIPREL
                     STPLFVCSRITGWCAHVMEQQEHNRLIRPRALYTGPAPRTYEPLDRRP"
     assembly_gap    1349..1358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1359..2242)
                     /locus_tag="PK614_07430"
     CDS             complement(<1359..2242)
                     /locus_tag="PK614_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRB82079.1"
                     /translation="MNQAIQDQPGTKTTDRPLYSPGLEGVIAGESALCQVDEVEAGLR
                     YRGYAIGDLAEWSRFEEVAYLLLIGHLPTRSELDSFYTLLRSHRVLPDPVQRFVDRLQ
                     PGLHPMDVLRTGISLLGLSDPDAQDGSHEANLRKSMRLLSQIPLLIAHSYRVMAGTVP
                     QRSDESRDFSQHLLRLVAGHKDGETGTAMAQALNVSLILYAEHEFNASTFAARVTAST
                     LTDLHGAITAAVATLKGTLHGGANEAVASMLIEIGSSERAETWVREALAQKRRVMGFG
                     HRVLRQGDARSAIIQRHA"
     gene            complement(2184..3125)
                     /locus_tag="PK614_07435"
     CDS             complement(2184..3125)
                     /locus_tag="PK614_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRB82080.1"
                     /translation="MRNHSTPSKAQRLRELLAGRTVAIPGACNALTAMQIERAGFEIV
                     YVSGAAISAARGLPDIGLISLTDMTREASAIARSVALPAIVDADTGYGPPSVVREAVQ
                     EFERAGLAGMQIEDQEEAKKCGHLSGKRLIPTADMVAKITAAVEAKRDRDFVLVARTD
                     ARTVDGLQAAIQRGLAYAEAGADALFPEALLSADEFHTFALEMNRAGVHVPLIANMTE
                     FGKTPYLSVDEFETLGYRGVLFPVSTLRVAALAVEKLLRELRFFGSQRAWLDHMMTRQ
                     ELYSLLRYEHGQDQTGRPYEPGDTRPARNENHGSAPL"
     gene            complement(3118..>3652)
                     /locus_tag="PK614_07440"
     CDS             complement(3118..>3652)
                     /locus_tag="PK614_07440"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82081.1"
                     /translation="KAEGADAIQRLSEIEIGSYDVAIEIIGRDPEKWHPTTRETADHS
                     FPYCVAAALLDGRVTLHSFDPNRLRDAALHELMKKVRVVPQPEFAGRYPGAMPTRLTL
                     RTTTGTVYMKQVDVPLGHPGNPMSDEDVEDKLRRLASRRIGRAQVEKLIGFVWALERE
                     KDIATLMPLLRVPHRDA"
BASE COUNT          682 a         1111 c         1116 g          733 t
ORIGIN      
        1 tcttcctgtg tgaggtcgtt cgggaggaaa caggaatagc aggtcgtttc agacagaccg
       61 tatccatggc ggatcgggaa tccaaaacgc tcttcgaagc gcagggccgt gtccttcagc
      121 agcggtccgg cgccgcagat cacaccgccg aagtgatcca ggcgataggc ggcgaggtct
      181 tcgtcggcat ccagaagaaa ctcgagcaag gttgggacga cgctgacact ggtcacctgc
      241 tcttcgtgga gtcgtcgcca gaagctgccg gtcttgaact tccggttcaa gacgacgctt
      301 cccttccaat aaaacggagt catcagagtg acgacgatgc cgttgacatg gtggacgggc
      361 aggacgcaca tcacacggtc ggtgagaccg aatccatgcc ggtcggcgat ggcatccgca
      421 tcgatcagaa gattggccac cgtcaagatg acgcctttcg gcggcccggt tgtcccggat
      481 gtgtaaatga tcaaggcagg atcgtcgagt cgggaggtgc ccggggcaaa gcgggctcca
      541 acggcgtggc tgttttttcg gctctttccc ctggtctcct tcccgtcaag gatgccaccg
      601 tcccccaggg cgaccacttc gcgcaaggcc ggcagcatgg actgaagagc tttgagttcc
      661 tcgtagacat cctgccagca gagggcgacc gaggcctccg aatgttcgag gatataccgc
      721 ttcttctcgg gtgtttcttc gacattgatc ggcacgacgg cgactcccaa cgtccaggct
      781 gcgaagtaga tcagcgccgt cagatcatgg ttgaacagca cggtggcgat ccggtcgccg
      841 cgggtcaggc cgaacttcgc atgaaacatc tccgccatcc gttcgacgat cgtgccaaat
      901 tccgcataac tgtaggttcg gcgcaggttc cggtcgtcgt cgcaataggt caggaaattc
      961 ctggtgacga gtcgcgggtc gtacaggcgc gatcggaaga actcggcgaa cgaggtccag
     1021 tggagttccg aggaacatcc cggcgcctcg cgggcctgtt cgatccggtc atggacgtcg
     1081 gtcaagaggc tcataggtcc tcggtgcggg ccctgtgtag agggctcgtg gtctgatcag
     1141 ccggttatgc tcctgctgct ccatcacatg tgcgcaccag ccggtgatgc gggaacagac
     1201 gaacaacggt gtggatagtt cacgcgggat gcccatcaac aggtaggcca cggctgtata
     1261 gaagtcgaga ttggggtgga gtcctttttc ctcccgcatg atgcggtgga gggtggaggc
     1321 gatgtcgtac caactgtggt cgccgcagnn nnnnnnnntc ggcgtggcgc tggatgatgg
     1381 ctgatcgtgc gtcgccttgc cgaagcacgc gatgcccgaa tcccatcact cgccgtttct
     1441 gcgcgagcgc ctctcgcacc caggtctcgg ctcgctcaga gctgccgatt tcgatgagca
     1501 tcgacgccac cgcttcgttg gcgcctccat ggagggttcc tttcaacgtc gcaacggccg
     1561 ccgtgatcgc gccatgcaga tcggtgaggg ttgaggcggt gacgcgagcg gcaaacgtgg
     1621 aggcgttaaa ttcatgctcc gcgtacagga tgagcgacac gttcagcgcc tgggccatgg
     1681 ccgtgccggt ttcaccgtcc ttgtggcctg ccaccaggcg cagcaggtgc tgcgaaaaat
     1741 ctcgactctc gtccgatctc tgcgggactg ttccggccat gactcgataa ctatgcgcga
     1801 tcagcagcgg gatctggctc agcagtcgca tggatttgcg gagattggcc tcatgcgatc
     1861 catcctgtgc gtcgggatcg ctgagcccca gcagggaaat gccggttcgc agcacgtcca
     1921 tcgggtgcag gcccggctgc aggcggtcta caaaacgttg caccgggtcg ggcaggactc
     1981 gatggcttct cagcagtgtg taaaaactat ccagttcgct ccgggtgggg agatggccga
     2041 tcagcagcaa ataggcgact tcctcgaagc ggctccattc cgccagatcg ccgatggcat
     2101 acccccggta acgaagtcct gcctctactt catccacctg acagagggcc gattctcccg
     2161 cgatcacccc ctcaagtccg gggctataga ggggccgatc cgtggttttc gttccgggct
     2221 ggtcttgtat cgcctggttc atagggcctc cctgtctggt cctgcccatg ctcgtatcga
     2281 agcagactat agagctcctg tcgcgtcatc atgtgatcga gccaagcccg ttgagagccg
     2341 aagaatctga gttctcgcag cagcttctca acagcaaggg ctgcgacccg gagcgtgctg
     2401 acgggaaata acacgccgcg ataccccaac gtctcgaatt cgtccacgct caagtaaggt
     2461 gtcttgccga actccgtcat gttggcgatc aagggcacgt ggacgcctgc tcggttcatt
     2521 tccaacgcaa aggtgtggaa ttcgtccgcg gagagcaggg cctcggggaa gagcgcgtcg
     2581 gcgccggcct cggcataggc gaggcctcgc tgaatggcag cctgcagccc atcgacggtc
     2641 cgtgcgtccg tgcgcgccac aaggacgaaa tcccgatcgc gtttcgcctc tacggctgcg
     2701 gtgattttgg caaccatgtc agccgttgga atcagtcgct tccctgacag atggccgcat
     2761 tttttcgcct cttcctgatc ttcgatctgc atgccggcca atccggcccg ctcaaattcc
     2821 tgaacggcct cccgcacaac cgaaggcggc ccatagccgg tgtccgcatc gacgatggcg
     2881 ggaagcgcca ccgaccgggc aatggccgac gcttccctcg tcatgtccgt caagctgatc
     2941 aatccgatat cgggcaggcc ccgggcggcc gagattgcgg ctcccgacac atacacgatc
     3001 tcgaatccgg cgcgctcgat ctgcatggcc gtcagggcat tacaggcccc ggggatggcc
     3061 acggtcctgc cggccagcag ctcacgcagg cgttgcgctt ttgagggagt ggagtgatta
     3121 cgcatcacga tggggcaccc tcaacagagg catcaaggtg gcgatgtcct tctctcgttc
     3181 caatgcccac acgaaaccga tgagcttctc gacctgcgca cgaccgattc gtctcgatgc
     3241 gagccggcgt agtttgtcct ccacatcctc gtccgacatg ggattgccgg gatggcccag
     3301 ggggacgtcc acctgcttca tgtagaccgt tcccgtcgtc gtcctcagcg tgaggcgggt
     3361 cggcatggct cccgggtaac ggcccgcgaa ttccggctgc gggacgacac gcaccttctt
     3421 catgagctcg tgcagcgcag catctcgtag ccggttaggg tcgaatgaat gcaacgtgac
     3481 gcgaccatcc agcaacgccg ccgcgacaca ataggggaag ctgtgatcgg ccgtttcgcg
     3541 cgtcgtcggg tgccacttct ccggatcgcg cccgataatt tcgatcgcga catcgtaact
     3601 gccgatttca atctcgctca gacgctggat tgcatcggcc ccctccgcct tg
//
LOCUS       DAORQN010000139         3631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_893_length_3631_cov_7.049497, whole genome shotgun sequence.
ACCESSION   DAORQN010000139 DAORQN010000000
VERSION     DAORQN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_893_length_3631_cov_7.049497"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..167
                     /locus_tag="PK614_07445"
     CDS             6..167
                     /locus_tag="PK614_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB82082.1"
                     /translation="MKVEDESVAVEAGSVIYVPPGAKQSLVNNGTAPIEFLCLVDPAW
                     TAEDEAVGE"
     gene            186..1262
                     /gene="leuB"
                     /locus_tag="PK614_07450"
     CDS             186..1262
                     /gene="leuB"
                     /locus_tag="PK614_07450"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRB82083.1"
                     /translation="MNAKIAVLAGDGVGREIVPEAVKVLKVVAERYGHTFEFISGDVG
                     GHAIDKVGVPLPAETLAIAKHSDAVLLGAVGGPKWEGLEYSLRPERALLGLREQLGLY
                     ANLRPAKLYPMLADASTLRRDVIEGIDMLVIRELTGGIYFGKPKGIEKLPGGGERGFN
                     TEVYTTEEIRRIAVVAFDAARKRRKKVTSVDKANVLESSELWRRVVTDVHKGYPDVAL
                     SHIYVDNCAMQLVRNPRQFDVLLCNNIFGDILSDEAAMLTGSIGMLPSASVGAKVGLF
                     EPIHGSAPDIAGKNIANPIATIASAAMMLSYAFQLDKEAAAIEQAIVKTLELGFRTKD
                     IHAEGMQLVGTVEMGDAIVRNLPS"
     gene            1266..2480
                     /locus_tag="PK614_07455"
     CDS             1266..2480
                     /locus_tag="PK614_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRB82084.1"
                     /translation="MTTRTTQSGIIETLAGNGEPGYAGDGGPAGAASLNEPKGLCVDR
                     EGNLYIADSENHVVRRVDRVTGIITTVAGVCPSGATGPAVPEPPVAAQAEEDEDPFAD
                     HSGDSTKAYTQVTDLSGTVRYVTGGRLTVEHDSGDGGPARRARLNFPSAVAVDRAGNL
                     YIADTMNHRVRKVDAATGIITNVAGTGQARYSGDDGPAVSAAINEPTGLAVSDEALYI
                     ADQSNNRVRRVDLATGVITTVAGDGTAAYSGDQVSAVQASLAGPSGVALGGDGVVYVA
                     DTFNSRIRSVDPATGQIATVAGDGGTYRYQGPAEPSSPSLSRPAGIAVGSDGNVYMTD
                     SDSHLIRVWNGRSKTISRLSGTGVAQFGGDGGDALAGSLSYPFGVAVDASGTVYVADT
                     FNHRIRVLTATA"
     gene            2491..2637
                     /locus_tag="PK614_07460"
     CDS             2491..2637
                     /locus_tag="PK614_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82085.1"
                     /translation="MLKKKQAYTVAILGATGAVGKESLEILEERNFPIETLRLFSSKR
                     SAMK"
     gene            complement(2916..>3631)
                     /locus_tag="PK614_07465"
     CDS             complement(2916..>3631)
                     /locus_tag="PK614_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRB82086.1"
                     /translation="MVILPNNAIGGANAFAHTSWMHQDVLLKDKTTYEIMRPESVGLE
                     QVKLVMGKLSGRHAFRQRLEDLGYKLTEEEVNHAFERFKRLADQKKEIYEEDLEVIVS
                     EEVAKMSERVVLKSFQVESGTNKTPTATVELEIDGKLVSHSGTGDGPVDAVYRTIAAM
                     TQTKSKLLMFGVNAITGGTDAQGEVSVRVEEDGRTVSGHGADTDIITAAARAYLNALN
                     KLAYFAAKQAEGIQKVSLI"
BASE COUNT          746 a         1010 c         1129 g          746 t
ORIGIN      
        1 gggtgatgaa ggtggaggac gaatcggttg cggttgaggc gggctccgtg atctacgtgc
       61 ccccgggggc caagcaatcg ttagtgaaca atggaacagc cccgattgag tttctctgcc
      121 tggtcgatcc agcctggacc gcggaggatg aggccgttgg tgaatagtcg agaggagatc
      181 tgcacgtgaa cgcgaaaatc gcagtcctgg ccggagatgg agtcggccgt gaaattgttc
      241 cggaagcggt caaggtgttg aaggtcgttg cggaacgcta cgggcatacg tttgaattta
      301 tatccggcga tgtcggcggc catgcgatcg ataaagtcgg ggtgccgctt ccggctgaga
      361 cgctcgccat cgcaaagcac agcgatgccg tgttgctcgg cgccgtcggc gggcccaaat
      421 gggaaggtct ggaatatagc cttcgtcctg agcgggcctt gctggggctg cgggagcaac
      481 tcgggctcta tgccaacctc cgaccggcca agctctatcc catgcttgcc gacgcctcca
      541 cgttacgacg tgacgtgatt gagggcatcg atatgctcgt gatccgggag ctgaccggag
      601 ggatttattt cggcaagccg aaggggattg aaaagctgcc gggcggcgga gagcgtggct
      661 tcaatacgga ggtctacacg accgaggaaa tccgtcgcat tgccgtggtg gcgtttgacg
      721 cggctcgcaa gcgccggaaa aaggtcacct cggtggataa ggccaatgtg ctggagtcct
      781 cggagttgtg gcggcgggtg gtcacggacg ttcacaaagg gtatcccgac gtggccttga
      841 gtcacatcta tgtcgataat tgcgcgatgc agctggtgcg taatccgcgg cagttcgatg
      901 tgttgctgtg caacaacatc ttcggggata ttctcagcga cgaagccgcg atgctcaccg
      961 ggtcgatcgg catgttgccg tccgccagtg tcggcgccaa ggtggggttg ttcgagccga
     1021 tccatggcag tgcgcctgat attgccggca aaaacatcgc caatccgatt gcaacgatcg
     1081 cttctgccgc aatgatgttg tcctatgcgt ttcagctcga caaggaagca gcggcgatcg
     1141 agcaggccat cgtcaagacg ctggaactcg ggttccgcac caaggatatt catgccgaag
     1201 gcatgcagct ggtcggcacc gttgagatgg gtgatgcgat tgtgcgcaac cttccctcgt
     1261 aagttatgac gacacgcacg acacaatccg gaatcattga gactctggca ggcaacggcg
     1321 agccggggta cgccggtgac ggtgggcctg ccggtgccgc ctcactcaac gagccgaaag
     1381 gcttgtgcgt tgatcgggag ggcaacctct atattgccga ttccgaaaac catgttgtgc
     1441 gtcgagttga tcgggtcaca ggcatcatta cgaccgtggc cggggtttgt ccgtcaggag
     1501 cgaccggacc ggcagtgcct gaaccgcctg tggcggcgca ggctgaggaa gacgaggatc
     1561 cctttgccga ccattccggt gatagtacca aggcctacac gcaggtgacc gatctgagtg
     1621 gcaccgtccg gtatgtgacc ggagggcgct tgacggtcga gcacgattca ggcgacggcg
     1681 gccctgccag gcgagcgcga ctcaatttcc ccagtgcagt agccgtcgat cgcgcaggca
     1741 acctctacat cgccgacaca atgaaccacc gggtgagaaa ggtcgatgcg gcgaccggga
     1801 tcatcaccaa cgtggccggc acaggtcagg cacggtattc cggcgatgat ggaccggcgg
     1861 tgtcggccgc gatcaatgaa ccgaccgggt tggcggtcag cgacgaggcg ttgtacatcg
     1921 cggatcaaag caataaccgt gtgcgtcggg tcgacctggc gaccggggtc atcaccacgg
     1981 tggccggaga tggaacggcg gcctatagcg gagaccaggt ttcggccgtt caagcgagtc
     2041 tggccggacc cagcggcgtg gcgttaggcg gagacggcgt cgtctatgtg gctgatacgt
     2101 ttaacagccg gatacgatct gtcgatccgg cgacggggca gattgcgacg gtagcgggag
     2161 acggcgggac ctatcgatac cagggaccgg ctgagcccag ttcgccgagc ctctccaggc
     2221 ctgctgggat tgccgtcggt tctgatggga acgtgtatat gacggattca gacagtcact
     2281 tgattcgggt gtggaacggg cgtagcaaga cgatctctcg cttgtccggt accggtgtcg
     2341 cgcagttcgg cggcgatggc ggcgatgctt tggcaggaag tctcagttat ccattcggtg
     2401 tagcggtgga cgcgtccggg accgtctatg ttgcggatac attcaatcac cgcatcaggg
     2461 ttctgaccgc gactgcgtaa ggacagcatc atgttgaaga agaaacaggc ttatacggtg
     2521 gcgattctcg gcgcgaccgg ggccgtggga aaagaaagtc tggagattct ggaagaacgc
     2581 aactttccaa tcgagacgtt gcgcctgttc tcctccaagc ggtcggcaat gaagtaatac
     2641 acttcggaag attttaagcg atgccagagg ctctgctgac ccggggcgag cagtccgtgc
     2701 gccaggctat atccgagcgc gagcgacgcc tttgccgggt gcagcaattc ccgcagcctg
     2761 gtgtgatcgc ccgccaagaa ttccgggcat cgttgaagat gcgtgtttag catcgtgcac
     2821 cgagatccat agtaggaatg acagacgatg tcgctccgtc cagtccgata cgaacgcatc
     2881 ggcccggacg gaggaatcta ccctgcaggt tgggctcaaa tcaagctgac cttctgaatc
     2941 ccttcagcct gtttcgcagc aaaataggcc aatttattga gcgcattcag ataggcccgg
     3001 gcagccgccg taatgatatc ggtatcggcg ccatggccgg agaccgttcg accgtcctct
     3061 tcgacccgca ccgacacctc cccttgcgca tccgtcccgc cggtaatggc attgacccca
     3121 aacatcagca acttgctctt cgtctgggtc atcgcggcga tcgtcctgta gaccgcatcc
     3181 accgggccgt cgccggttcc gctgtgagag accagtttcc catcgatttc caattccaca
     3241 gtcgccgtag gagtcttatt ggttccactt tcgacttgaa acgacttcaa caccacccgt
     3301 tccgacatct tcgcgacttc ctcagacaca atgacttcga gatcttcctc ataaatctct
     3361 ttcttttgat ccgccagccg cttgaatcgt tcaaatgcgt ggttgacctc ttcttccgtc
     3421 aacttatagc ccaaatcctc gagccgctga cgaaacgcat ggcgcccgga caacttgccc
     3481 atcaccagct tgacctgctc aagaccgaca gattcaggcc gcatgatctc gtaggtcgtc
     3541 ttatctttca gcaacacatc ctggtgcatc cacgacgtgt gggcgaaggc attggcaccg
     3601 ccaatggcat tgttcggtag gataaccata c
//
LOCUS       DAORQN010000140         3603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_896_length_3603_cov_11.509019, whole genome shotgun sequence.
ACCESSION   DAORQN010000140 DAORQN010000000
VERSION     DAORQN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_3603_cov_11.509019"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="dapA"
                     /locus_tag="PK614_07470"
     CDS             complement(<1..804)
                     /gene="dapA"
                     /locus_tag="PK614_07470"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRB82087.1"
                     /translation="MFTGSLVAIVTPFKNGKLDEKSLGDLIEWQITSGTQGIVPCGTT
                     GESATLTHAEHDRVVAFTVEVARRRVPVIAGTGSNSTQEAITLTKHAKAAGADGALLI
                     TPYYNKPTQEGLFLHYKAVAEAVDLPLVLYNIPGRTGVNMMPSTISRLTVCPTIVAIK
                     EGSGSVQQASEIIQLCGERLTVLAGDDALTLPMMAVGGKGVITVTANLVPSDMAELVN
                     AFLAGRVDDARAMHYRLYPLFTALFYETNPIPVKEALHMMGKIDREMRLP"
     gene            complement(841..2103)
                     /gene="lysA"
                     /locus_tag="PK614_07475"
     CDS             complement(841..2103)
                     /gene="lysA"
                     /locus_tag="PK614_07475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRB82088.1"
                     /translation="MNDFQYREGELYCEDVPLSRIAKEVGTPCYVYSHHTLVRHFRVY
                     DSAFQNIPHIVAFAMKANSNLAVLRLMAKEGSGVDIVSGGELFRALKAGVSPAKIVFA
                     GVGKKAEEIRDALKADILMFNVESSAELQAINDVAASMGVKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRAIEEFKAAAAFSHIEVVGLHAHIGSQLTQVAPFVESLKKVL
                     AMVQTLAEQGIPIRYLNIGGGLGITYSDETPPEPKDLAAAIFPLVRDLKCILIMEPGR
                     VIVGNAGVLLTKVLYTKDGETKRFLIVDAAMNDLIRPSLYDAHHDIRPVYENVARAAK
                     QTVDVVGPVCESGDFLAKDRVMPEMNAGDLMAVMSAGAYGFVMSSNYNSRPRVPEVLV
                     HEGQIHVIRARESYDDLVHGEQIPAFLS"
     gene            complement(2505..3599)
                     /gene="argH"
                     /locus_tag="PK614_07480"
     CDS             complement(2505..3599)
                     /gene="argH"
                     /locus_tag="PK614_07480"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRB82089.1"
                     /translation="MFGRLGGKLHTGRSRNDQVALDLRLFLRDVLSTLMGQLQEFRRV
                     LVGQARAHLDVVMPGYTHLQRAQPVLLAHHFLAYVEMFDRDRGRLHDCRQRLDVMPLG
                     SGALAGSNYPVDRRYTAALLEFPAVTQNSLDAVSDRDAVVETLSALSLIMMHLSRLSE
                     ELILWASQEFRYVDLPDTFCTGSSMMPQKKNPDVPELVRGKTGRVYGHLMGTLTLLKG
                     LPLSYNRDLQEDKEALFDAVDTTGQSLALCTELMRRLVVNKTVLAEAAEGGGMLATEL
                     ADYLVTKGVPFREAHSITGQIVRFSLEQHRPLQQLTLKDLRGFSAHFGEDVLSCLTVR
                     GAIDRKGQIGGTARRRVEARIKELEKALKG"
BASE COUNT          809 a         1175 c          894 g          725 t
ORIGIN      
        1 cggcagccgc atctcgcggt cgatcttgcc catcatatgc agcgcttctt tcacaggaat
       61 ggggttggtt tcataaaaga gggccgtaaa cagcggatag agccgatagt gcatcgcccg
      121 cgcatcatca actcgaccgg ctaaaaatgc attgaccaac tcggccatat cactgggcac
      181 cagattggcc gtcaccgtga tcactccctt gccacccact gccatcatcg gcagggtcaa
      241 cgcgtcatct cccgccagca cggtcagacg ctccccgcaa agctgtatga tttcggatgc
      301 ctgctgaacc gagccgcttc cttctttaat ggcaacaatc gttgggcaca ccgtcaatcg
      361 tgagatggtg cttggcatca tatttacgcc ggttcgccca ggaatgttat agagaacaag
      421 gggtaaatcg actgcctccg cgaccgcctt gtagtgcaga aacagccctt cctgcgtagg
      481 cttattgtaa taaggcgtga tcagcagtgc gccatcggcc cccgccgctt tcgcgtgctt
      541 ggtcagagta atcgcttcct gagtactatt tgatcccgtt cccgcgatca ccgggactcg
      601 tcgtctggca acttcaaccg taaacgccac cacacgatcg tgctcggcat gtgtcaacgt
      661 ggcagactcg ccggtagtgc cgcaggggac aatcccttgc gtaccgctgg taatctgcca
      721 ttcgatgagg tccccgaggg acttttcatc gagcttaccg ttcttaaacg gcgtcacaat
      781 ggcgaccaga gatcctgtga acatgggagg ctccataagg aagacagtta ttactgaggt
      841 ctaggaaaga aacgccggta tctgctcgcc gtgcaccagg tcgtcataac tctcccgcgc
      901 acgaatgaca tggatctgtc cctcatgaac cagcacctcc ggcacacgag gtcgcgaatt
      961 ataattcgag gacatgacaa acccataggc gcccgcactc atcacggcca tgagatctcc
     1021 ggcattcatt tccggcatca cccgatcttt cgccaaaaaa tcgccggact cgcacacggg
     1081 cccgaccaca tccacagtct gcttcgccgc acgcgcgacg ttttcataga ccggccttat
     1141 atcatgatgt gcatcataca ggctggggcg aatcaggtcg ttcatcgccg catccacgat
     1201 cagaaaccgt ttggtttcgc cgtccttcgt gtacaacacc ttcgtgagca acacgccggc
     1261 attcccgaca atcacgcgac caggctccat gatcaggata cacttcaggt cacgcacaag
     1321 cgggaaaatc gccgccgcca aatctttcgg ttccggaggg gtttcatctg aatacgtaat
     1381 ccccaggcca ccgccgatgt tcagataacg aataggaatt ccctgctcgg ccagggtctg
     1441 caccatcgcc aacactttct tcagcgactc gacgaaggga gccacttgcg tcaattgcga
     1501 cccgatatgt gcgtgcaaac ccaccacttc gatgtggctg aacgctgcgg ctgccttgaa
     1561 ctcttcgatg gcacgatccg ccgcgatccc gaatttactc ttcttcaaac ccgttgaaat
     1621 atagggatgc gttttgggat cgatgtccgg attgatgcgc aacgccacgc gcgccttgac
     1681 tcccatggac gccgcgacgt cattgatggc ctgcagctcc gcagaagatt ccacattgaa
     1741 catgagaata tccgccttca aggcgtcgcg gatttcctcc gcttttttcc caacgccggc
     1801 gaacacgatc ttggcgggag acacccctgc cttcagcgca cgaaatagtt ctccgcccga
     1861 cacgatatcg acgccactcc cttccttggc catcagacga agcaccgcca aattggaatt
     1921 ggccttcatg gcaaaggcga caatgtgagg aatgttctga aaggcactat cgtagacccg
     1981 gaagtgccgg acgagcgtgt ggtggctata cacgtaacaa ggggtcccca cctctttcgc
     2041 aatgcgcgag agcggcacat cctcgcaata gagttcacct tcccgatact gaaagtcatt
     2101 catggtggtc atcctcgatc aacaattcta tacccgcctc ggagaatccg tccaatagcc
     2161 acaacgccaa ccggtcgaaa ccacgaccac aacctcgact cacatctatc gcgtacccat
     2221 cgtccttagt ggcggagtcg gcaacgggtc gggatccgga ggaatcgggg cttcttcaac
     2281 ggtcactgca ctcggcgcgc tgggcctgga agcagatccc cacacattcg cgcctggcgc
     2341 taacagccct tcacgtctca actgccgctc aatcaccggt gccacaccga cgtcttcagg
     2401 ggggatcggc gcacccaata ccccgcatcc gcccagtccc acaacaatcc acactgcacc
     2461 gacggcacca cccctgactc ccgggccaca ggccctgacg agcctcatcc cttcaacgcc
     2521 ttctcaagct ccttgatccg tgcctccaca cgccgtctcg ccgtgccacc gatctgacct
     2581 ttccggtcga tggcgccccg cacggtgagg caactcagga cgtcctcgcc gaaatgggcg
     2641 gagaaacctc gcaagtcttt caaggtcaac tgctgaaggg gccgatgctg ctcgagtgag
     2701 aatcgcacaa tctggccggt aatggaatgg gcctcccgaa acgggacccc cttcgtcacc
     2761 aggtagtccg ccagctccgt cgccagcatt ccgccacctt cggccgcctc agccaggacg
     2821 gtcttattga cgaccagcct ccgcatcaac tccgtgcaaa gcgccaacga ttgccccgtc
     2881 gtatccaccg cgtcgaacaa tgcttcttta tcttcctgaa gatcgcgatt gtaactcagc
     2941 ggcaatccct tgagcagcgt cagggtgccc atcaagtgac catagactcg acccgttttc
     3001 ccacgcacca actccggcac gtccggattc tttttttgcg gcatcatact gctgccggtg
     3061 cagaaggtat cgggcagatc cacgtaacga aattcctgcg acgcccagag aatcaattct
     3121 tcgctcaagc gtgagaggtg catcataatc agcgacaagg cactgagtgt ctccaccacc
     3181 gcgtcacgat ccgagacggc atcgaggcta ttctgcgtca ccgcagggaa ttccagcagt
     3241 gcagctgtat atcgacggtc gaccgggtag ttcgatcccg ccaacgcccc cgaccccagc
     3301 ggcatcacat cgagccgttg ccgacagtcg tgaagccgac cccggtcacg atcgaacatt
     3361 tctacatagg ccaggaaatg atgcgccagc agcaccggct gggcacgctg caaatgtgta
     3421 tacccgggca tgacgacatc cagatgcgcc cgggcctgcc ccacgaggac tcggcggaat
     3481 tcctgcaatt gacccatcag cgtcgaaagc acatcgcgca aaaatagacg cagatccagg
     3541 gccacctgat cgttgcggct tcgacccgtg tgcagctttc cacccagccg gccgaacaac
     3601 taa
//
LOCUS       DAORQN010000141         3603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_897_length_3603_cov_5.716460, whole genome shotgun sequence.
ACCESSION   DAORQN010000141 DAORQN010000000
VERSION     DAORQN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_3603_cov_5.716460"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PK614_07485"
     CDS             complement(<1..1339)
                     /locus_tag="PK614_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB82090.1"
                     /translation="MRQQLPAMMPEPSELVRGALPDPGPRLERIPESPEWGEFERGVA
                     LYNQGQYLEARLHLTNMLREHRDLPLRSSIQAFLTESSLKSSAQELRPLEIIDQYKAL
                     IREDPQSTNARRAAWRIGDVYRVEGWYQEAQIAYQHALSLSERDSYDANRAMLGLGYV
                     FRGIKSWKDSVQTFDHVLKRTTDPTLLVSASLGQAHSLYRMGRIKDADALYESISSRW
                     PGALRADPFALLRYADTAGEAHRGPVMREQLLHFYNLYPSRPENPFVLMHLADSYKEA
                     GRWEDASIFYAALLSQYPDAQVVPTAKLRYAEVQEHLAPEGEEVNLRHTIAAHLANVP
                     LKPGEMLSPRQLFESSAKQYEDSPVGSEALFHLGEALERAGALKAYEQVVQRAGKFEN
                     DPWPEKGGAQLVRFLRPRLEAALKADDDFELINLFHRNGPFADRLYAGTELLLK"
     gene            complement(1636..3051)
                     /locus_tag="PK614_07490"
     CDS             complement(1636..3051)
                     /locus_tag="PK614_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB82091.1"
                     /translation="MSQSHILVIDDDPAVRQILAETLAGEGHQVTVMSSGLDGVEAVK
                     DQPVHVVLTDLQMPGIDGLETIDRISKIDSKIIAIVMTGYGTVDYAVRAMKAGAFDFI
                     TKPFEPDTVAVVVRKALDVYKLKQENHLLRKAVRDQYRLEHLVGTSAPMRMVLDFVEK
                     VADSDSTVLIEGESGTGKELIARMLHFNSMRRERPLVPVNCGAIPETLLESELFGHEK
                     GAFTGAAHTRLGRFELAHGGTIFLDEVGEMSLPLQVKLLRVLQERCFERVGGTRTINV
                     DVRIIAATNQDLAQAVQERRFRQDLYYRLHVIPIHIPPLRERRSDIPLLVNHFIAQFN
                     QLRRTEILGMEPDALARMMEEEWPGNIRELENMIERLCVLKKRGMITMSDLPERPVKM
                     NAAKGGESPEQFIRFSEDGINLTKELEHYENRLIGEALRKANGITSRAAQLLQVNRTT
                     LVEKLKRKGFDPKTHGYSVQN"
     gene            3359..3434
                     /locus_tag="PK614_07495"
     tRNA            3359..3434
                     /locus_tag="PK614_07495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3392..3394,aa:Thr,seq:tgt)
BASE COUNT          822 a         1048 c          900 g          833 t
ORIGIN      
        1 ccttaagcag cagttccgta ccggcgtaca gccgatcggc aaacgggccg ttacggtgga
       61 acaggttgat gagctcaaaa tcgtcgtctg ctttaagtgc cgcttccagc cgggggcgca
      121 gaaatctcac gagctgcgcc ccacccttct ccggccaggg atcgttctca aatttccctg
      181 cccgctggac cacctgctcg taggctttca gagcgcctgc ccgctccaac gcctccccga
      241 gatgaaataa cgcctcgctt ccaaccggtg agtcttcata ttgcttggcg ctgctttcga
      301 acaactggcg cgggctgagc atttcaccgg gcttgagggg aacattcgcg agatgtgcgg
      361 caatcgtgtg ccgaagattg acctcctcgc cttcgggagc gagatgctcc tgcacctccg
      421 catatcggag cttggccgtg ggcacaacct gcgcatccgg atactggctc aacaacgccg
      481 cataaaaaat gctcgcatct tcccatcgcc cggcctcttt gtaactgtcg gccaggtgca
      541 tcaacacaaa tggattctcc ggcctggagg gatacaggtt gtagaaatgg agaagctgtt
      601 cccgcatgac cggtccgcgg tgggcttctc cggctgtatc cgcgtaacgc agcaatgcaa
      661 acgggtccgc ccgaagagcg ccaggccagc ggctgctgat gctttcatac aacgcatcag
      721 cgtccttgat ccgtcccatt cgatagaggc tgtgggcctg tcccagcgac gcggacacca
      781 gcagcgtggg gtccgtggtt cgtttcagga cgtgatcaaa cgtctgtacg ctgtccttcc
      841 aactcttgat gcctcgaaag acatagccca gaccgagcat agctcgattg gcatcgtagg
      901 aatctcgctc ggagagactg agcgcatgct ggtaggcgat ctgcgcctcc tggtaccaac
      961 cttcgacacg atacacatcg ccgattctcc aggcggcccg cctggcattt gtcgactgag
     1021 gatcttctcg aatcagggcc ttgtactgat cgatgatttc cagaggcctc agttcttgtg
     1081 cgctactctt taaggagctt tctgttagaa atgcttgaat agacgaccgg agcggaagat
     1141 cgcgatgctc tctcaacatg ttagtcaggt gaagccgcgc ctcgagatac tgcccctgat
     1201 tgtagagggc cactccccgt tcaaactcac cccattcagg actctcggga atccgttcca
     1261 agcgaggccc tggatccgga agtgcgcctc gaaccaattc cgacggctca ggcatcatgg
     1321 caggtaattg ctgtcgcatc gtcgagggtc caccggcagc cataacagaa gagcccccag
     1381 cgaggaacag actacccagg aaaaatgcca gacctatacg gatcctgacg ggttgttgtg
     1441 gtgaagatcg gtattgcaca ggaggcaaat cagcaagtcc tgtgcccttt ctggaatcat
     1501 ccaaatagcg gcatcacttg aggatttcgg tcagcagcga aaactggaca gcaggtgttg
     1561 tcagaaatcc cgacaagcac tttgtggggc cggtcaaaaa aatgacgtgt gagaggagag
     1621 aacgtgtgag gaacgttagt tttgaaccga atacccgtga gtcttgggat caaatccttt
     1681 ccgtttgagc ttttccacca gtgtggttcg attcacttga agcaactgtg cggctcggct
     1741 ggtaattcca ttggcctttc gcaacgcttc cccaataagc ctattttcat agtgttcgag
     1801 ttctttggtc aaattgatcc cgtcttccga aaagcgaata aactgctcag gcgactcgcc
     1861 tcccttcgcg gcattcattt tgacgggccg ctcaggcaaa tcgctcatag tgatcatgcc
     1921 ccgtttcttc aacacacaga gacgttcgat catgttttca agttcccgga tattccccgg
     1981 ccattcttct tccatcatgc gcgctaacgc atcgggctcc atacctagaa tctccgtccg
     2041 ccgcaattga ttaaactggg cgatgaaatg gttgaccaga agcggaatat cgctacggcg
     2101 ttcccgcaat ggcggaatgt gaatgggaat cacgtgcagc cggtaataca agtcttgtcg
     2161 gaaccggcgc tcttgaaccg cttgcgccaa atcctgattc gtcgccgcaa tgatacggac
     2221 atcgacattg atcgtccttg tcccgcccac gcgctcgaag catcgctctt gcaacacccg
     2281 cagcagtttg acctgtaacg gcaaactcat ctccccgacc tcgtccaaaa agatggttcc
     2341 cccatgagcc agctcgaatc gcccgaggcg cgtatgggcc gctccggtaa aggccccctt
     2401 ctcatgcccg aacagttcgg actctaacaa ggtttctgga atggccccgc agttgaccgg
     2461 gaccagcgga cgctccctcc gcatgctgtt gaaatgtaac atccgcgcga tcaattcctt
     2521 gccggtccca ctctcacctt caatcagcac tgtgctgtcg ctatcggcca ccttttcgac
     2581 aaaatcaagt accatgcgca tcggtgcgct cgtccccacc agatgctcca ggcgatattg
     2641 atctcgcacg gcttttcgaa gcagatggtt ttcctgcttc aacttgtaga cgtccaatgc
     2701 ttttcggacc actaccgcca cggtatcggg ctcaaagggc ttggtaataa aatcaaatgc
     2761 gccggctttc attgcccgta ccgcgtagtc gactgtccca taacccgtca tgacaatcgc
     2821 aataatcttg gagtcgattt ttgagattcg atcaatggtt tccaacccat cgatccccgg
     2881 catctggaga tccgtcagca ccacatgtac cggctgatcc ttgacagcct ctactccgtc
     2941 cagcccactg gacatgaccg tcacctgatg tccttcacca gcgagcgtct ccgccaagat
     3001 ttgacgcaca gcaggatcat catcaatcac gagaatatgt gactggctca tcaagggcac
     3061 tcctctctga ggggaccaaa gaaatcaggt tctgctaaat aaggatgacc agaacgcact
     3121 acgagaaaca actctctcgt tgaatggttg aacacagaat cagcttattg ggaattgaaa
     3181 ctttattcaa gtgaaagtaa ggatggcctg catagctgag ccatcagatc ctacacaaag
     3241 cgagcttctc tctccgatcg cacccagcct agatcttgac aggtgagaag aagaattgta
     3301 gggttcgaaa tcttcctttc agattcactt ggtattaatc aggtagctga tctcgcgggc
     3361 tggcgtagct caattggcag agcagcggtt ttgtaaaccg ccggttgggg gttcgattcc
     3421 tctcgccagc tccacaaatc agacggacgt gtcaagcacg tggcggctcg cgaactcact
     3481 cctcgtatca cgcggttctt taattttccc cactgtagtt caaattagtc cggaaggtac
     3541 gctgaagaac cttcaaactc tcgttgcttc acaacagtgt ggcaaccgta tacacgacat
     3601 aca
//
LOCUS       DAORQN010000142         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_901_length_3588_cov_6.714973, whole genome shotgun sequence.
ACCESSION   DAORQN010000142 DAORQN010000000
VERSION     DAORQN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_3588_cov_6.714973"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK614_07500"
     CDS             <1..277
                     /locus_tag="PK614_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82092.1"
                     /translation="PIGNGIPESHQPNIKALILSPKEPNVIYAMTNHTIHKSINGGEN
                     WKTLEHNMTGSFAMQFSAIGVDPSSHSIYVGSWGGVFRINDDDSAPN"
     assembly_gap    328..337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    939..948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <949..2866
                     /locus_tag="PK614_07505"
     CDS             <949..2866
                     /locus_tag="PK614_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82093.1"
                     /translation="MGRYRILGSLFSLCVIALLVISAACSVPPYKNSNRQLTEEQKAE
                     ALRFLNPVPNQPSRPDEILASTVPELTERAMKDLGSRNDIVLVVFMARNDVTTTLYKI
                     RDTMQSMDPKSSLNPYWVTDVPGFVGDPQTKCGVVVGHWYSDGKYPYRAPWERIVDAV
                     LERETTFGTYLYSHNGKFFLTEDGQSDMEALQDVCFPGHPLASTRFESFGAANQSRPA
                     LSRWKPTSAPEEYATVQTTTKDVAAKTLMGDTLVGQACGNTVNLNLRITDVFLHNSAE
                     QNDPMYLIKGVLKVSRNAQASPEIALVGAISRVNGVVNLAASSEGPNPRYRNTIISLF
                     RDDADSGLEGMIEGNEEIGAAGCDDVRLTSKNGTKLTAFPKMTAQVALFFSNPQMAPR
                     SKLYPVYWLKVAEAHGSIDAPYYLGSRYEQRGATDPNAYAPAVQYYSAAATAYDDARA
                     QAALGRMYEKGQGTKRDPEKAKQLGNAAKTTRQRAARVCASSQVREVIERFLTQVSLQ
                     NKAGSETMNTLTGIRPDRGEFTISKVTAEDVVSFNYSFICRAKGGFDNPNFHIEMPEK
                     RITVWRDRDGYETMTDNSMEQLSSQAAPVLMNEAARNVVNYHTFNLKPLGNNRFKITY
                     RGPVGMQSEEVQVN"
     gene            2890..3012
                     /locus_tag="PK614_07510"
     CDS             2890..3012
                     /locus_tag="PK614_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB82094.1"
                     /translation="MRQSKFTETQIVAILKEADASRSVNEIWRHYGISSATYYK"
BASE COUNT          922 a          965 c          946 g          735 t
ORIGIN      
        1 tcctattggg aatggtattc cagaatccca ccagccaaat atcaaggctc ttatattgag
       61 cccaaaagaa cctaatgtta tctatgcgat gacaaatcac accattcata agagcataaa
      121 cggcggcgaa aattggaaga ccctggaaca taacatgact ggctcattcg caatgcaatt
      181 cagtgccatt ggagttgatc catcgtctca ttccatttat gtgggatcat ggggaggagt
      241 tttcagaatt aacgatgatg atagcgctcc gaactagaag acgaacttat ctatgtgatt
      301 tgcacacatc gcttcacata aactgccnnn nnnnnnnacg ttggggacag ctctatcagg
      361 aaatgggaaa cgccgggatc gtatgtcgtc gatacgggat ctcctatccg acagttcgga
      421 agtcgatgcg cgggtaccgc gacgccggag aggccaattt cacaccatca cggatttagt
      481 gttgcaccct cttagtccgt aaattctcta cgctgggagg acaagggaat tcgtaccatc
      541 aagaatgtta ggccgagggg aatgtggccg gcccgcgatt tctgtaccaa gtctaaggag
      601 agtctccagc agcgagagcg aggatatgac ctttctcccc agaaaactag actactgaca
      661 gtgtgcgcac ggccatattt ggaagatcac agggagcagg gaccatccga catactgtcc
      721 ctgcatcgga gcgcggaaat acgcgcctgc ctgagcatct tgacgtccgg caatgatctg
      781 ccgatctgga tgacacttcg aatgatcggc ccgggctaag catctcatct caccacactc
      841 gacaaaccgt gaaacacgct ctctttctcg gctcgaagca atatgctctg gataccacca
      901 cgtaaccaac gcaactgctg aaagggtacc cgaatgcann nnnnnnnnta tgggcagata
      961 cagaatcctt gggtcgctct tctctttatg tgtcatagca ctcttggtaa tttccgccgc
     1021 atgttccgtc ccgccgtata aaaactcaaa ccggcaacta actgaagaac aaaaagccga
     1081 agcactacgg tttctgaacc ctgtacccaa tcagccgtcc agaccagacg agattcttgc
     1141 gagcactgtt ccagaactga cggagcgagc gatgaaggac ctcggctcac gaaacgatat
     1201 agtgctggtg gtgtttatgg cacgaaatga tgtgacgacc acactctata aaattaggga
     1261 caccatgcag tccatggatc cgaagtcgtc cttaaacccc tactgggtga cggatgtccc
     1321 aggatttgtc ggagacccac aaacgaaatg tggcgttgtg gtcggtcact ggtattctga
     1381 tggaaaatat ccatatcgtg cgccatggga acgtattgtg gatgcggtct tagaacggga
     1441 aacgacgttt ggcacatacc tttactcgca caatggaaaa tttttcctga ccgaagatgg
     1501 tcaatccgac atggaggcgc tacaagacgt ttgtttccca gggcacccct tagcctcgac
     1561 tcgtttcgaa tcgtttgggg cggccaacca aagccgtcca gccttgtcgc ggtggaagcc
     1621 cacatctgcg cctgaggaat acgccactgt gcagacgacg acaaaagatg tggctgccaa
     1681 gacactcatg ggcgacacgc tcgtgggaca agcttgcggc aacactgtga atcttaacct
     1741 gaggatcacg gatgtctttc tccataacag cgctgagcag aatgacccga tgtacttaat
     1801 aaagggggtc ttaaaggtct ctcgcaatgc acaggcttcc ccggagatcg ccttggtagg
     1861 tgccatttca cgagtaaatg gcgtcgtgaa tctcgcggcg tcaagcgaag gtcccaatcc
     1921 gcgttacaga aacaccatca tttcgctttt tagagatgat gcagacagtg ggttggaagg
     1981 tatgatcgag ggcaatgagg aaattggggc tgcggggtgc gacgacgtcc ggctcacgag
     2041 caagaatggg acgaaattga ctgcgtttcc caagatgacg gcccaggttg cgctcttctt
     2101 ctccaatccc caaatggctc ctcgatcaaa actgtatcca gtctattggc tcaaggtcgc
     2161 ggaagcccat ggcagtattg acgcaccgta ttacctggga tctcgatacg aacagcgcgg
     2221 cgccacggac ccgaatgcct atgcccccgc ggtgcagtat tactcagcag cggccaccgc
     2281 ctacgatgat gcaagggcgc aagccgcttt agggcgcatg tatgaaaagg ggcaaggaac
     2341 aaagagagac ccagagaagg cgaagcagtt aggcaacgcc gcaaagacca cccgacaacg
     2401 cgctgcgcgc gtatgcgcct cctcgcaagt cagagaagtg atagaacgtt ttctaacgca
     2461 ggtctcgctc cagaacaaag ctgggtctga gacgatgaac actctgactg ggattcgccc
     2521 ggaccgcggg gagttcacga tcagcaaagt gacggctgaa gacgtcgtat cgtttaacta
     2581 ctcgtttatc tgtcgtgcca aaggcgggtt cgacaatccc aattttcaca ttgagatgcc
     2641 tgagaaaaga attactgttt ggagagaccg agacggctat gaaaccatga cggacaattc
     2701 aatggaacag ctttcgtcgc aagcagcacc cgttctgatg aacgaagccg ccaggaatgt
     2761 ggtgaattat cacacgttta atttaaaacc attgggcaac aatcgtttca aaataacgta
     2821 tcgtggccca gtaggcatgc agtcggagga agtccaggta aactaagctc gcgcgtttac
     2881 catatcttag tgcgccagtc gaagtttacc gaaacccaaa ttgtggccat cctcaaggag
     2941 gccgatgcca gccgctccgt caacgagatc tggcgacact acgggatcag ctccgccacc
     3001 tactacaagt gaaagaccaa gtacggcggg ctggaggcct ccgacctgaa gcggctgagg
     3061 gagccggaac acgaaaatag tcgcctcaag cggatgtatg ccgatctctc gttggagaat
     3121 gcggtgctga aacatgtcat cgcaataaag ctctaaggcc ctctgaccgc cgggaacttg
     3181 tgacacatct cgccacggtg aacggcctcc ccgttcagcg ggcctgccgt gcgtagggat
     3241 ggaccgagcg acgtactacc gacgacttct ctttccgccc tcgtcagtgt acagtcaccc
     3301 cgtgcgaccg catcccgaca cggcgctccg cttgacccga tgagcatgcc cggcaaaccg
     3361 cacttcacac aacgagatcc ggcgtcgtcc gacagggtgc ctcctccccg aaagccggcg
     3421 cggagcatgg cacgcgccaa ggcaaccagc gcgaaacgtg tgaccctgga tcgcctgctc
     3481 tccaaactgg gcattaccag ccggagccag gcgcaggaat ggatcaaggc gggacgggtc
     3541 cgcattaacc agcgcctggt acgcacgccg gacacctggg tcgactgg
//
LOCUS       DAORQN010000143         3526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_914_length_3526_cov_46.225295, whole genome shotgun sequence.
ACCESSION   DAORQN010000143 DAORQN010000000
VERSION     DAORQN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_914_length_3526_cov_46.225295"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK614_07515"
     CDS             <1..519
                     /locus_tag="PK614_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB82095.1"
                     /translation="VHPAPPASTKVHGKTYTLQTSQNPYRVNLEGKFDQAYSNKLIVE
                     QGMGRLMAPNANPWDDKAEGYLKYVREGGEIFFQNCHFCHGDNLNGRGLHAFAFNPIP
                     ANFTDPGTIAQLQETFIFWRVAKGGIGLPNEGFPWASVMPPWEQHLTTDEIWKVILFE
                     YWHTGYYPRTWD"
     gene            582..2351
                     /locus_tag="PK614_07520"
     CDS             582..2351
                     /locus_tag="PK614_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB82096.1"
                     /translation="MNVKATPIIGAALGALILSASAVGLGHAGDLPEGFSKGELAPAP
                     PAEMIEAGKRVYFTKCVWCHGVDGAGDGPGADRLWPRPRNFNQGTFKIRHTASGELPL
                     FNYNEKTPDSGKNDLLDTVTHGLPGSAMPPWEGILTEEQRLQVLSFVTTQLVKDRKFT
                     DKQSETQTILQLGTIKPAEPTEESIKKGAQLIVDKKCIECHGVEGRGDGNAFNLKDDW
                     GFSIQPADWHKCWNFRGSRQDPYNVRNIFRTFSTGVNGTPMPSFADSTSVEDRWSIAN
                     FVNSLCERDAEGKPLGIDPLTDKPKINFVVPSAPVEGEISNDPENEMWKKQGRRYVAM
                     GGQITHKPRNFVNRIDDIWVRSLYNEKNVVYLIQWDDRTKSVAEGKLPWAPTQVNVEN
                     FGVKEQAPKTGEEGSIAAAQNNYAVYNDGIAFQFPIKWQEIPAPFKPRYLWGDAKFNA
                     DILKWEADGSLRSFKGTGWDQDFEERDDFEEKVKLLKSEWKNGQWTVMISRPLKGDKD
                     DYDEYTRFDIGKYIPMVFFAWDGHNGDAGRKMAVSAFYYTILQPPIPQEVYIYPAVIA
                     VGVVFLEGWMLTRRANKKKGKVL"
     gene            2444..3064
                     /locus_tag="PK614_07525"
     CDS             2444..3064
                     /locus_tag="PK614_07525"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRB82097.1"
                     /translation="MSERTLVNVTGVLIAGGKSRRMGRDKRFLKVNGESVFDRTLSLL
                     MDTFVETVVVLAEPIESLEVRGCRVVYDVIPNAGSLGGLYTGLLAATQPRIFAVACDM
                     PFLHAEVIRYMVSCDETADVVVAELEGQFQPMHAVYSKRCAEFLKAMAERQNLKIQTL
                     YRSEVLRVAVLGANDLSSLGVGLRSFQNINTPEDLALADAPISDKP"
BASE COUNT          825 a          831 c         1026 g          844 t
ORIGIN      
        1 gtacatccgg caccgccagc tagcacgaag gtgcacggca agacctacac cctgcaaacg
       61 tcacagaatc cttaccgggt taatctcgag ggaaagtttg accaggcgta cagcaataag
      121 ctgatcgtcg aacaggggat gggacgactg atggcaccca atgccaaccc atgggatgac
      181 aaggctgaag ggtatctgaa gtacgttcgg gaaggtggcg aaatattctt ccagaattgt
      241 cacttctgtc acggcgacaa tcttaacggc cgcggtctcc atgcctttgc gttcaatccg
      301 attcccgcta acttcacaga cccgggaacg attgcacagt tacaggaaac cttcattttc
      361 tggcgcgtag ctaagggcgg aattggtttg ccgaacgaag gattcccgtg ggcctcggtt
      421 atgccaccat gggaacagca cctgacgacg gatgaaattt ggaaggtgat cctgttcgag
      481 tattggcaca cgggatacta tcctcggaca tgggactaat cctgaatctt acaaacaacc
      541 tgggagaaca ggtggtgagg ttaacaatga gcaaacttcg tatgaacgta aaggcgacgc
      601 cgattatcgg agcagcctta ggggccctta tcctctccgc gagtgcggtt gggttgggcc
      661 atgctggaga tcttccggaa ggcttttcaa aaggggagtt ggcacctgct cctcccgccg
      721 agatgatcga agctggaaag cgtgtctatt tcaccaagtg tgtctggtgt cacggcgtgg
      781 atggtgctgg tgatgggcca ggtgccgatc gtctgtggcc acgaccgaga aatttcaacc
      841 agggcacatt caaaatccgt cacacagcga gcggagagtt gcccctgttc aattacaacg
      901 agaagactcc tgattctggg aagaatgacc tgctcgatac ggttacgcat ggtctccccg
      961 gttccgccat gccaccgtgg gaaggtatct tgaccgagga acagcggctt caggttttgt
     1021 cgtttgtcac gactcagttg gtcaaggatc gaaagtttac tgacaagcag tctgagacac
     1081 agacgattct tcaacttgga accatcaaac ctgctgagcc gactgaagag agcattaaga
     1141 agggcgccca gctgattgtc gataagaaat gtattgagtg ccatggtgtg gaagggcgtg
     1201 gggatggaaa tgccttcaat ttgaaagatg actggggctt ttcaattcaa ccggccgatt
     1261 ggcacaagtg ttggaacttc cgtgggagtc gccaagatcc ttataatgtg cggaatattt
     1321 tccgtacctt ctccacgggt gtaaacggga cgccaatgcc gtcattcgcg gatagcacgt
     1381 ccgttgaaga ccgttggagc attgcgaact tcgtaaattc gttgtgtgag cgcgatgccg
     1441 agggtaagcc tcttggcatt gatcctttga ccgacaaacc gaagatcaac ttcgttgttc
     1501 cttctgctcc agtcgagggt gaaatatcga acgaccccga gaatgagatg tggaagaagc
     1561 agggccgtcg ttatgttgcg atgggcggtc agatcaccca caagcctcgt aacttcgtca
     1621 atcgtatcga tgatatctgg gtcaggtcgt tgtacaacga gaaaaacgtg gtgtacctga
     1681 tccagtggga tgaccggacg aagagtgtcg ccgaagggaa acttccttgg gcgcctactc
     1741 aagtcaatgt tgagaacttt ggtgtgaagg agcaagcgcc taagaccgga gaagaagggt
     1801 cgattgctgc tgctcagaac aactatgccg tctataacga tggcatcgcg ttccaattcc
     1861 cgatcaagtg gcaagagatt cctgccccat tcaagccacg atatttgtgg ggcgatgcga
     1921 agttcaacgc cgacattctg aagtgggagg cggatggttc tctccggtcc tttaagggca
     1981 ctggttggga tcaagacttt gaagaacgtg atgacttcga agaaaaagtt aagttgttga
     2041 agtcagagtg gaagaacgga cagtggactg tcatgatctc ccgtccgctg aagggcgaca
     2101 aggatgatta tgatgagtac acgcggttcg acatcggtaa gtacattccg atggtcttct
     2161 ttgcctggga cggacacaac ggagatgcgg gacggaagat ggccgtatcg gccttctatt
     2221 acacgatctt gcagcctccg attccacagg aagtgtatat ttacccagcg gttatcgcag
     2281 ttggcgtggt gtttttggaa ggctggatgc tgacccgccg agccaacaag aagaagggta
     2341 aggtcctcta agtcgatact cgagctagag gtggtgtcgc gagggggtgg ggaaactcac
     2401 cccctcgttt tttatgtgac taggatttgg ggactacatt gggatgagcg agcgcaccct
     2461 agtcaatgtc accggggtcc tcattgccgg tggaaagagc cggcgcatgg ggcgggataa
     2521 acgcttcctg aaggtgaacg gagagagtgt ttttgaccgg acgctctctc tattaatgga
     2581 tactttcgtg gagacggtgg ttgtcctcgc tgaaccaatc gaatcactgg aggttcgtgg
     2641 ctgtcgagtt gtctatgatg ttatcccgaa cgcaggtagt ttgggtggtc tgtataccgg
     2701 gctattagcg gccacacagc cgaggatatt tgccgtggcc tgtgacatgc cgtttcttca
     2761 tgccgaggtg attcgttaca tggtctcctg tgatgaaacg gccgatgtcg ttgtcgcgga
     2821 actcgaggga cagttccaac cgatgcacgc agtgtattcc aaacggtgcg ccgaatttct
     2881 caaggcgatg gctgaacgtc aaaacttgaa gatccagaca ctgtatagaa gtgaagtatt
     2941 acgagttgca gtgttaggag cgaatgacct gtcatctctt ggggttggtc ttcgttcatt
     3001 tcagaatatc aatacgcccg aagatttagc actcgccgat gcaccgatat ctgacaaacc
     3061 gtgacgagcc aggttccaca gggcggcaac aagcgcgggg tgctagtcat tcaccccggg
     3121 gccctaggtg atgtgctctt gtcccgccct gctttatgcg cagtccggca ccagtttcct
     3181 caccatgaga ttgcgttttt ggccggacag tccgtgggag cgctcttgtg tggagctggg
     3241 gaagttgatc gagtttttcc tcttgaatcg tcatatttga gtgggctatg ggctgggccg
     3301 gacagccttt gttcagaatt tagggcatgg cttggcaatt gcgatattgc agtcggatgg
     3361 cttccggacg cagaaggggc cctctcggct acacttcggg cctcgggtgt tcagtatgtc
     3421 tgcctgcaat ctgctgcatc taccgatctg tgtgccgaac accaagcgtc acgatatctt
     3481 gatgccattc aactgagcgg gactagtcag ctagtcggta ctccac
//
LOCUS       DAORQN010000144         3512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_917_length_3512_cov_9.710732, whole genome shotgun sequence.
ACCESSION   DAORQN010000144 DAORQN010000000
VERSION     DAORQN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_3512_cov_9.710732"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK614_07530"
     CDS             complement(<1..376)
                     /locus_tag="PK614_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82098.1"
                     /translation="MEWRGCELGNGIWRGIRYWCAIGLLVGGLPACSTTQSAEKTEPV
                     VTGTTSVKPAEAERPARETPKASKSSERTSKSGSKTVKSQAAAKEKGSEQATAKSDAP
                     IRQPEAGSQVAKKPDAPLTETPG"
     gene            complement(805..2046)
                     /locus_tag="PK614_07535"
     CDS             complement(805..2046)
                     /locus_tag="PK614_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02270 family protein"
                     /protein_id="HRB82099.1"
                     /translation="MKQVIDSIVQQHAEEAAFLWLLRNNAVHAPHYSLRDLAKLDGRV
                     EAHLDGLRVAGEPGWDLCKTALGNEENGEVFAASVMAFESGIESRIQAVLEALQKTPE
                     LSKGLISAFGWMPFEQAAPHAQRLLAADSPLKRLIGLAAYAVHRQDPGAPLKEALSSP
                     DLSLKARALKAVGELGKANLLPFVKSNLAVEDLTCRYWAAWSGALLGEPAAIPVLQRL
                     AESGHAQREVACAMALRRMSVQEGQAWLKVLASHPETLRLAIQAAGVIGDPAQISWLI
                     QQMANHAIARVAGESFTCITGVDLAYDDLDTDKPDGFEAGPTENPEDESVDMDPDEDL
                     PWPNPQLIERWWFTHRVQFTNGTRYLCGQPMAIESLNQVLRTGKQRQRTAAAIELAIR
                     QPGTPLFNTEAPGFRQQALLK"
     gene            complement(3151..>3512)
                     /locus_tag="PK614_07540"
     CDS             complement(3151..>3512)
                     /locus_tag="PK614_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82100.1"
                     /translation="DSPTNLILERVICAPSSTVYVCGQNGVVLRGRGMQFETIKQTTV
                     EDDFWGMEWFEGKLWLATHESLFTLNEDELIPVDMGVGEPLTCGWLHANDGVIWSVGP
                     EDLAFYDGKTWSEVILT"
BASE COUNT          792 a         1045 c          885 g          790 t
ORIGIN      
        1 cgccaggagt ttccgtcaac ggcgcatctg gcttcttcgc tacttgcgaa cccgcctccg
       61 gctgacgaat cggggcgtct gatttggccg tagcctgctc cgaccctttc tcttttgcgg
      121 cagcttgaga cttcaccgtt ttgcttccgc ttttcgaggt tcgttctgaa gacttcgaag
      181 ctttgggagt ctcacgagcg ggtcgttcgg cttcagccgg tttgacagaa gtggtacccg
      241 tcacaaccgg ttcggtcttt tcagcggact gtgtcgtcga gcaagcaggc aagccaccga
      301 caagcagccc gatcgcgcac caatatcgaa tacctcgcca gatgccgttg cccagctcac
      361 atccgcgcca ttccatcgca tgcctctact cggccttgct acgaaaggac atgaagactc
      421 gctcgccaac agaacaggag aagggcccgt gttatttccc cccccttctc aacggtcttc
      481 tgtcccacca ccccgtcatg gttcaacgtg cgactatcct tctgaaactg cgcaacaacc
      541 cgtcgggttt cctccccata gattccatcg gggcggtata agggattgcc ggtagagagt
      601 ttggcatggc atagccgaga tccaacagcg ccatctgtag aagatgcaca cctctccccg
      661 ccgaaccgac ttggagccta gcgacaatca acgagaccct attcaggatg gcgctcgact
      721 tgaagcgtgg aggtacaaga gccatgatgc tctccttggt gaggtggacc gcacgaatct
      781 aaactgtctg ccctgctata ccctctattt caacagcgcc tgttgccgga aaccgggcgc
      841 ctcggtattg aagagcggcg taccgggttg gcggatggcc agttcaatcg ctgctgcggt
      901 gcgttgacgc tgcttgccgg ttcggagaac ttggttgagc gattcaattg ccatcggctg
      961 gccacagaga taacgcgtcc cgtttgtgaa ctgaacccga tgggtgaacc accacctttc
     1021 gatcaactgc gggttgggcc aaggcaagtc ttcgtccgga tccatgtcca cgctctcgtc
     1081 ttcgggattc tccgttggcc cggcttcgaa accatccggc ttatcggtgt ccagatcgtc
     1141 ataggcgaga tcgactccgg taatgcaggt gaaggattct cccgccaccc gagcgatagc
     1201 atgattcgcc atctgttgaa tgagccagga aatttgtgcc ggatcgccaa tgactccagc
     1261 tgcctggata gctaggcgaa gcgtttccgg atggctcgct aagactttga gccatgcctg
     1321 cccctcttgc acagacatcc gtcgaagcgc catcgcgcag gcaacctcac gctgggcatg
     1381 tccgctttcg gcaagccgct gtaacacggg aatcgccgct ggctcgccga gcagcgcacc
     1441 agaccaagcc gcccaatagc gacaggttag gtcttcaacg gctagattcg atttgacgaa
     1501 tggaagcaga ttcgccttgc cgagttcgcc cacggcttta agcgcgcgag ctttgagcga
     1561 aagatccggc gaggagaggg cttctttgag cggagcgcca ggatcctgac gatgaacggc
     1621 ataggcagcg agtccgatca gtcgtttaag aggcgagtca gctgcgagca gtcgctgtgc
     1681 atgaggggct gcttgttcga acggcatcca tccgaaggcg gagatcaagc ccttgctcaa
     1741 ctctggggtc ttctgcaggg cttcaaggac ggcctggatg cgtgactcta taccggactc
     1801 gaatgccatc acggaggcag caaagacttc gccgttctcc tcattgccga gcgcggtctt
     1861 gcagagatcc cacccaggct cacccgccac gcgtaaaccg tcgagatggg cctcgacacg
     1921 gccatccagc ttagccaggt ctctcagcga atagtgcggc gcatggacgg cgttattgcg
     1981 aagcagccat aaaaaggcgg cttcttcggc gtgctgctgg acaatgctgt caatgacttg
     2041 tttcatagcg taccgctaac agacatatcc atgcttgctg atcaaccacc aaagccggac
     2101 tccgcctcgc gtcactcact acagcattac cgacgatctt cagatcttca agcagcatct
     2161 cagataacgg ccatccatgg atcactggct gccggcttgc gcaacgcggc tttcctccac
     2221 tccagcggac agctctctca cgtaacagag gcgcttattg tccgtttgag attcgaagcc
     2281 ctcgccggaa cacttcgtgc cagggatcga ccccactcgg tcacagtaat ctgctcacct
     2341 catgaacccc caagacatta gacactctgt tgttcatgtg gccatagtac ctcgatatca
     2401 agcttttcac tccgacagag gttgacaaag gaaccttgga catctaagag atgagcagcc
     2461 cctatgacca cagcgatgaa caagtttgtt gagtcgactg aacggagcct ttccatgagt
     2521 acagaaaaca tgtactcgtc cctcccagag cctatggaac ggccaacttc tcttgccttc
     2581 aatgaatcgt cagacaggct tgacaggcga cgtgattcgt tcaaatagat tccgctagcc
     2641 cgtaccatcg tttcctgacc acttgccccg gctggaatat ccaatctggg taagagccaa
     2701 tcatcgatat cagtaattgt cgtttgaacc gcacgccgaa ctataccatt gccagtaata
     2761 gccacatcat ctctgtcgcg accatcaagt aaccaaagta cgcccgattt cggcggcacg
     2821 gagtccggta gatcaccttc gtacgcaagc gtgtgcccca tcgcggtaca gaactcgata
     2881 ctcagttctg gtctcttttc tttaagaagc gaccctaagg caccgcgctt cggaacgagg
     2941 gcgttatagg tagtctgagc ccactcaaga ccaatggcgt caatttgtcg gcctcgctca
     3001 tttgacagtc ccctgaggac ttggatcaag cgattctttc cgaaaggatc gtggtggaac
     3061 gctccaagaa gaaccaaccg gatcaaagcg acaccgaatg ctttagccgt aatgggctag
     3121 agctccaact gatactgatt ggccatcaga ctacgtcaaa atgacttctg accatgtttt
     3181 accatcatag aatgctaaat cttcgggacc gactgaccat attacgccat cgttggcatg
     3241 gagccaccca catgtgagtg gctccccaac ccccatgtct accggaatca gttcatcctc
     3301 attcagggta aatagggact catgcgtggc taaccacagt ttaccttcga accattccat
     3361 cccccagaag tcatcctcca ccgtagtctg cttaattgtt tcaaattgca ttcctcttcc
     3421 gcgcaacacc acaccattct gcccacagac atatacagta gaagaaggag cacaaatgac
     3481 cctttctaga attaggtttg tgggactgtc ga
//
LOCUS       DAORQN010000145         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_920_length_3482_cov_4.692442, whole genome shotgun sequence.
ACCESSION   DAORQN010000145 DAORQN010000000
VERSION     DAORQN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_3482_cov_4.692442"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK614_07545"
     CDS             complement(<1..525)
                     /locus_tag="PK614_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82101.1"
                     /translation="MASSLITPSRAKRNKPTIGFVNLGCSKNQVDSEVMLGTLVAGGF
                     QLTGDARAAEVVIINTCGFIEEAKQESINSIIEHGRLKKSGSCRVLIAAGCLAQRYQG
                     ELLKELPELDGVVGTGEFGRIAEICRNLLAPKARQQRLWIGQPPYLYDAETPRIRLGT
                     PHSAYLKIADGCNRN"
     gene            610..1149
                     /locus_tag="PK614_07550"
     CDS             610..1149
                     /locus_tag="PK614_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRB82102.1"
                     /translation="MIRTFQGITPTIPQSCFIEETAVIIGDVVMGEECSAWFHAVIRG
                     DVNYIRIGHRTNVQDLCMLHVTHDTHPLIIGDDVTIGHNVVLHGCTIHNRVLVGMGAI
                     IMDGAVIGEDSVVGAGALITAGTVVPPKSLILGSPAKVKRPVTEQELAWIRESAQNYI
                     RYSRQYLSGPDKPRPGFWV"
     gene            complement(1189..1887)
                     /locus_tag="PK614_07555"
     CDS             complement(1189..1887)
                     /locus_tag="PK614_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82103.1"
                     /translation="MARKLMNVGTAARRAMREVFARLDYARLGPIYCDEGGDEFWAAK
                     RGLCQRLGLKLAKALLPRLQPGGTSLYVGAGVAEIPLLLMESLDLGRHVLACNLRRQE
                     VLVLNRACAGFPIRFHHLDAGSVKDRVDHLWLVSVLNDPERFPELSALSYGRANPVTF
                     DARKFLPQQRTVATLVDRCLSRLVIPGSVTTTVEEAVWVAEWCHRRDIRYRIDRQTFA
                     SPTVGDPICLIRLG"
     gene            complement(2027..>2931)
                     /gene="argB"
                     /locus_tag="PK614_07560"
     CDS             complement(2027..>2931)
                     /gene="argB"
                     /locus_tag="PK614_07560"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRB82104.1"
                     /translation="APLKERFAQNVVLLKYVGLNPVIVHGGGPQIDQMLDRLGMVAKF
                     RHGVRVTDAATMEIVEMVLAGRINMEIVDLLNRHGGQAVGLSGKDGGLMLTKPLTAKA
                     WAESIEKDLDSDDSDADFGFVGDVKSIDPTLLLKLQEDNYIPVIAPIGTDREGNTYNI
                     NADLVAGAIAAALKAEKLVMLTDVKGIRDANGRHLPTVSRKDVQRMVKRGTISEGMLP
                     KVHACLDALAGGGGEGAHHRWPHAPCHSAGNLHSQGYRHRNCRVASDGAASQPSTGGG
                     AERAPAMVTAPCRGGRRRPWRRPR"
     assembly_gap    2932..2941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3154..>3482)
                     /locus_tag="PK614_07565"
     CDS             complement(3154..>3482)
                     /locus_tag="PK614_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82105.1"
                     /translation="HILFIAAGAFHVAKPSDLIPELQGRFPIRVELAPLTKDDFVRIL
                     TEPRGALVRQYQALMTTEGLAVEFTDDGLAEIAATAVEVKTSVPGGCLRSWSGCWNRC
                     RSKGPR"
BASE COUNT          711 a         1145 c          912 g          704 t
ORIGIN      
        1 gttccggtta cagccatcgg caattttcaa ataggcgctg tggggagtcc caagacggat
       61 gcgcggggtc tcggcatcgt acagataggg cggttgtcca atccagagtc gctgctgacg
      121 agctttcggc gccagcaagt tgcgacagat ctccgcgatg cgcccgaact cgcccgttcc
      181 cacgacgccg tccaactcag gcaattcttt caacagttcg ccctggtacc gctgcgccag
      241 gcatcccgcg gcaatcagga cgcggcagga tccggacttc ttcaaccgtc catgttcgat
      301 gatgctgttg atcgactcct gcttcgcctc ctcgatgaag ccacaggtat tgataatcac
      361 gacttccgcg gcgcgcgcgt cgcccgtgag ttgaaagcct ccggcgacca atgtgccgag
      421 catgacttcg gagtccacct ggttttttga acaaccgagg ttcacgaagc cgatcgtggg
      481 tttgttgcgt ttcgcccgtg atggggtaat caatgacgag gccataacct agcagtctac
      541 gagacgccgg aaaactctgt caaacgagct ctcttgctat tccgtctgca gctatcctaa
      601 attgagcgca tgatccggac atttcagggc atcaccccga ccatcccgca gtcctgcttt
      661 attgaagaga ccgccgtgat catcggcgac gtcgtcatgg gcgaagagtg tagcgcctgg
      721 tttcatgccg tcattcgcgg cgatgtgaac tacattcgca tcgggcaccg aacgaacgtg
      781 caggacctct gcatgttgca tgtcacacac gacacgcatc cgctcatcat cggcgacgac
      841 gtcaccatcg ggcacaacgt ggtcctgcat ggctgcacca ttcacaaccg cgtgctggtc
      901 ggaatgggcg ccattatcat ggacggagcg gtcatcgggg aagactcggt agtcggcgcc
      961 ggagcattga tcaccgcagg cactgtcgtt ccgcccaaga gtctcatcct gggctcaccg
     1021 gccaaagtaa aacgccccgt caccgagcag gaactcgcct ggatccgaga gtcggcccag
     1081 aactatatcc gctattcgcg ccaatacctc tcaggccccg ataaacctcg ccccggattc
     1141 tgggtctagt actattcacg cgcatacccg cattcgatac gtcgcttctc acccgagccg
     1201 aataagacaa atggggtcac cgaccgtcgg tgatgcgaag gtctggcggt ctatccggta
     1261 acggatgtcc cgccgatggc accactcggc gacccacact gcctcctcaa ccgtcgtcgt
     1321 caccgatccc ggaattacca atcgactcag gcaacgatcc accaatgtgg caaccgtgcg
     1381 ctgctgaggc agaaattttc gcgcatcgaa cgtcaccgga ttcgcgcgac cgtaggacag
     1441 cgccgataac tcggggaacc gttcgggatc gttcaggacg ctgaccagcc agagatgatc
     1501 cacccggtct ttcaccgatc cggcatccag atgatgaaac cggatcggga acccggcaca
     1561 ggcgcgattg agaaccagca cttcctgcct tcgtagatta caggccagca catgtcgccc
     1621 gagatccagg ctttccatga gaaggagcgg aatctcagcc acccccgcgc ccacatatag
     1681 actggtgccg cccggctgta gtcgcggcag gagcgccttc gccagcttca gaccaagccg
     1741 ctgacaaagt ccgcgcttcg ccgcccaaaa ctcatccccg ccttcgtcac aataaatcgg
     1801 acccagtcgc gcgtagtcga ggcgcgcgaa gacctccctc atcgcccgcc gagcggccgt
     1861 ccccacgttc atcagtttac gggccatgat gctccacacc ggccacacct gttgccgcga
     1921 tacctgcgcc aacgactacc gggctcataa gattgagagt taacctatct cgcgtgtcgc
     1981 tcgattgacg gcaatgcgca tatctgacgt tagccgcgac tggacactac cgaggacgcc
     2041 gccagggcct gcgccttcca ccacgacacg gcgccgtcac catcgcaggg gcccgctctg
     2101 cgcccccacc tgtggatggt tgagaagccg cgccatcact ggctacgcga caatttctgt
     2161 gccgataccc ttgtgagtga agatttccag cagaatggca tggggcgtgc ggccatcgat
     2221 gatgtgcgcc ttccccaccc cccccggcca atgcatccag acaggcgtga actttgggta
     2281 acatgccctc gctgatcgtg ccgcgcttga ccatccgctg cacatccttg cgggacaccg
     2341 tcggcaggtg gcgaccattg gcatcgcgga tgcctttcac atcggtcaac atcacgagtt
     2401 tttccgcctt caacgctgcg gcaatcgcgc ccgccacgag gtcggcgttg atgttgtacg
     2461 tattgccttc ccgatccgtt ccgatcggcg cgataacggg aatgtaattg tcttcctgga
     2521 gcttcaagag cagcgtggga tcgatcgatt tcacatcgcc cacaaacccg aaatcggcat
     2581 ccgaatcatc cgaatcgagg tccttttcaa tgctttccgc ccaggccttc gccgtcagcg
     2641 gcttggtcaa catcaacccg ccgtccttgc cgctgaggcc gaccgcctgt ccgccgtgtc
     2701 gattcaagag atcgacgatt tccatattga tccggccggc cagtaccatc tccacaattt
     2761 ccatcgtggc cgcgtcggtc actcgcaccc cgtggcgaaa cttcgcgacc attcccagtc
     2821 gatccagcat ctgatcgatt tgcgggccgc cgccatgcac gatcacgggg ttgagcccga
     2881 catacttcag caacaccaca ttctgggcaa atcgctcttt caggggcgca tnnnnnnnnn
     2941 ncccccgtac ttgatgacga ccgtcttgcc cttgaaggtg cggatgtagg gcagggcctc
     3001 gatcagcacg tccgctttct taatcaattt gttcatgcgc tccccccgtc cagcctgccg
     3061 cttacagaat atagcggctc aagtcttgat ccttcacaat attcagcaag cgctcccgca
     3121 catacccggc gtccacgacg atggtctttt ctttcatctc ggacccttcg aacgacacct
     3181 gttccagcaa ccgctccatg atcgtaaaca gccgccgggc accgatgttt tgacctctac
     3241 cgccgtcgcc gcaatctctg ccaatccgtc atccgtaaac tcgacggcga gcccttctgt
     3301 cgtcatcaac gcctgatact gacgcaccag cgcaccccgc ggttcggtca ggatacgcac
     3361 gaagtcgtct ttggtgagcg gcgccaactc gacgcggata gggaagcggc cctgcagttc
     3421 gggaatcaaa tccgacggct tggcgacatg aaacgcgccc gcggcgatga agagaatatg
     3481 gt
//
LOCUS       DAORQN010000146         3446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_932_length_3446_cov_5.219109, whole genome shotgun sequence.
ACCESSION   DAORQN010000146 DAORQN010000000
VERSION     DAORQN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_932_length_3446_cov_5.219109"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..>1120)
                     /locus_tag="PK614_07570"
     CDS             complement(195..>1120)
                     /locus_tag="PK614_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82106.1"
                     /translation="VFFVADASGFDHPWDLEVVQLQSSRLEIPREAPFMLPFNQWYTN
                     RPGTTLFMPVTNIALLYLNLLLTMFSEDTGYFIVDTDNGNAACGLDAFRKSAGGHLHD
                     NLASRKMFSLRELDAGICETAIQEQGIICEHLSLMQQALGLGGGIQSVGSGRHLLGME
                     PHIYPGLGFHFVVPSGKPLRANPVGIPQVWEGPTPPFVPSMKDAVTSLVASKFGPNGT
                     YGKPSEQPWTNPKAAQQVPQHSERAIEATIAFADYVLGTYGRFPAHADACKSIVASQT
                     HHLDEEFYATFYPDSTLPDAHREHMHVWHNH"
     assembly_gap    1121..1135
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1136..1470)
                     /locus_tag="PK614_07575"
     CDS             complement(<1136..1470)
                     /locus_tag="PK614_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82107.1"
                     /translation="MKQLAAHPAAMEATCTLLDLLQQRKTRRFGRGMTLPGGPLQYTS
                     HHEPIPLSREEERYLIYAAIGRSGRNLGDMQFVGRPGTSAGQGHALMNFKSRTVPSPC
                     SAQTTQLFYT"
     gene            complement(1690..>2083)
                     /locus_tag="PK614_07580"
     CDS             complement(1690..>2083)
                     /locus_tag="PK614_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB82108.1"
                     /translation="YFAFSNFDAKQADKLRLLLDILTPHFHLSFMRCNSAWSRERPTQ
                     PATVLSKREEEILRWVAAGKTNWEISVILKVSLNTVKFHLKNVFQKIGVENRWSAIAY
                     WQTGEHHRIIPPAPPSDNRPPSGPNTTD"
     assembly_gap    2084..2109
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2110..2497)
                     /locus_tag="PK614_07585"
     CDS             complement(<2110..2497)
                     /locus_tag="PK614_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82109.1"
                     /translation="MSQPAFSAKEFQRVGEIMHDARSVDRSDRMWQLVSAVQRVIPYE
                     FSGCGAVDLLHGIDPSLGHSTYPREFCQLYMGQGLVVDPAVQRLITSGQTVTSSADEP
                     AADEPKGITSLKLDFGIKTCLSAGVRG"
     assembly_gap    2843..2852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2853..>3446)
                     /locus_tag="PK614_07590"
     CDS             complement(<2853..>3446)
                     /locus_tag="PK614_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82110.1"
                     /translation="PFQDRLRWQDLVPIVNCGDHKVCVESATVKGMKWLELGIYARIG
                     EYHIYQSWHLSEDGELRPVVQSRGLSCNTDHVHHPYWRFDFDINGNGMDQVFVRDDGG
                     PDKGWGPGWRKYTNERNDVKNSATHRSWFVRDQLTGHGVWVLPGDGYAPLKDDGERDS
                     FADLDVAIRRANPDEDLPWVFGARGQLGYDEDNQGVQ"
BASE COUNT          651 a          916 c         1096 g          732 t
ORIGIN      
        1 ggcctgaaac ggtgaggtat cgaacgagcg gatcggtttc acggcgctct cgcctttgct
       61 caacagatgc caaaacaggt cgacagtaca gcccagcggg gaaatcactc ccattccggt
      121 gatgacgact cgacgagagg tctgtggtgt catggtgccc tcctctgctc tcaagagcgt
      181 gaaaatgtgt gtcgtcaatg gttatgccaa acatgcatgt gctcgcgatg tgcatccggt
      241 aacgtggagt cgggatagaa cgtcgcataa aactcttcgt ccaggtggtg ggtttgactg
      301 gcgacgatcg acttgcaggc atcggcgtgc gccgggaacc gcccgtatgt gccgagcacg
      361 tagtcggcaa atgcgatggt cgcttcgatg gcgcgctcgg aatgctgcgg cacctgctgc
      421 gctgctttgg gattggtcca aggctgctcg ctgggctttc cgtaggtgcc gttcggcccg
      481 aacttgctgg cgaccaggct ggtcacggca tccttcatcg acggcacgaa cggcggggtg
      541 gggccttccc acacctgcgg aatgccgacc ggattggcgc gcaggggctt gcccgacggg
      601 acgacgaagt ggaacccgag gccggggtag atgtgcggtt ccattccgag gaggtggcgg
      661 ccgctcccta cgctctggat gccgccgccc agtcccaggg cctgctgcat cagcgagaga
      721 tgttcgcaga tgatcccttg ctcttgaatg gcggtttcgc aaatgccggc gtcgagctcg
      781 cgcaggctga acattttgcg cgaggcgaga ttgtcgtgca gatgcccgcc cgcgctcttg
      841 cggaaggcat cgaggccgca ggcggcgttg ccgttgtccg tatcgacgat gaagtatccc
      901 gtgtcttcgc tgaacatcgt cagcaggaga ttcagataca ggagcgcgat attggtgacg
      961 ggcatgaaca gggttgtgcc ggggcggttc gtataccact gattgaacgg cagcatgaac
     1021 ggggcttcgc gcgggatctc caaccgggac gattggagtt gcacgacctc cagatcccag
     1081 gggtggtcga agccggatgc gtcggcgaca aaaaagacgc nnnnnnnnnn nnnnngtgta
     1141 aaacagttgt gtggtctggg cggagcaggg gctgggaacc gtgcggctct tgaagttcat
     1201 cagcgcgtgg ccctggccgg cactcgtgcc cgggcgtccg acaaactgca tgtcgcccag
     1261 gttgcgcccg gatcggccga ttgccgcata aatcagatat cgttcttcct cccggctcag
     1321 cgggatcggt tcatggtgac tggtgtattg cagggggccg ccgggtaggg tcatgccgcg
     1381 gccgaagcgg cgtgttttgc gttgttgcag gaggtcgagc agagtacatg tggcttccat
     1441 tgctgcagga tgtgcggcaa gctgcttcat agggcctctc cttccggcac gttagggcgg
     1501 tacggccccg tccggtgtgt gaataacggt ggtgctggtc cgtcatgcga ggcatgacgt
     1561 tgtgtggatt gaatgtacga gagacgtgtt ccggcgtcac ctacccatcg gggtaggcgg
     1621 gctcgctacc ccgagtcggt caactcgtga cggacggggt ggggcgcgac tcgacatgcc
     1681 tggggtgatt taatcggttg tgttgggccc agagggagga cggttatcac tgggcggcgc
     1741 gggtggaatg attcgatggt gctcgccggt ttgccaatag gcaatggcgc tccatcgatt
     1801 ttcgacgccg attttctgaa agacgttctt gaggtggaac ttcacggtgt tcaaactgac
     1861 ctttaggatg acggagattt cccaattggt cttgcccgcc gccacccatc gaagaatttc
     1921 ctcttctcgt ttgctgagga ccgttgcagg ctgggtaggt cgttcccggc tccatgcgga
     1981 gttgcaccgc ataaaactca ggtgaaaatg cggcgtcaga atatcgagca ggagtcggag
     2041 tttatcggcc tgtttcgcat caaaattact gaaggcaaag taannnnnnn nnnnnnnnnn
     2101 nnnnnnnnnc cccacgcaca ccggccgaga ggcaggtctt gatgccgaaa tcgagtttga
     2161 gcgaggtaat ccccttgggc tcgtcggcgg ctggttcatc ggcgcttgag gtgacggtct
     2221 ggccggacgt gatgaggcgc tgcacggctg gatcaactac caatccctgg cccatataga
     2281 gctggcagaa ttctcgggga taggtggaat gtcccagtga aggatcgatc ccgtgcagca
     2341 gatcgaccgc gccgcatcct gaaaattcat agggaatgac gcgctgcacg gccgatacga
     2401 gttgccacat tcgatcgctt cgatcgacgg agcgcgcatc atgcatgatc tcgccgactc
     2461 gctgaaattc ctttgcggaa aatgccggct gactcatgtc ggttcttccc gatcggcggg
     2521 cccctgtgct agtgggagca cctgagacca tgaagattca gaagcgcgga cgtggttcat
     2581 agtgagacag ctgtgccttc ttgagacaag ctgattgtac tgcttgctcg cgatcagtat
     2641 ctagggcaac ctacacctag tatagagtgc ttaaggtatg aaccgcaacg gagaagagga
     2701 gtgctcggga gaacgaacat ggaaccggcc ggaggttcgg aagccgttcg gctatcgatg
     2761 aatcttcaag gtcggtccga gggtcagcca cttcgtgggg ccgagggcgg ccatgtgcgg
     2821 gaggtgcgcc acgtaccaga acnnnnnnnn nntcctggac gccttggttg tcctcgtcat
     2881 aaccaagctg gccgcgggct ccgaagaccc aaggcagatc ttcgtccgga ttggcgcggc
     2941 gaatggcgac gtcgaggtcg gcaaacgagt cgcgttctcc gtcgtctttg agcggggcgt
     3001 acccgtctcc ggggagaacc cacacaccgt gtcctgtgag ttggtcgcgc acgaaccagg
     3061 accggtgagt ggctgaattt ttcacgtcat tacgttcatt ggtatatttt ctccatccgg
     3121 gtccccagcc tttgtccggc cccccatcgt cgcgtacaaa cacctgatcc attccgttgc
     3181 cgttgatgtc gaaatcgaag cgccaatagg gatggtggac gtgatcggta ttgcaggaca
     3241 accctcggct ctggacgacc ggccgcaatt caccgtcttc ggacagatgc caggattgat
     3301 aaatgtgata ctcgccgatt ctggcataga tgccgagttc cagccacttc atgcccttca
     3361 cggttgcgct ctcgacgcag accttgtggt ctccgcagtt tacgatcggg accagatcct
     3421 gccatcgcag gcgatcttga aagggg
//
LOCUS       DAORQN010000147         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_934_length_3437_cov_5.597575, whole genome shotgun sequence.
ACCESSION   DAORQN010000147 DAORQN010000000
VERSION     DAORQN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_934_length_3437_cov_5.597575"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PK614_07595"
     CDS             complement(<1..815)
                     /locus_tag="PK614_07595"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRB82111.1"
                     /translation="MHERPARRVVVTGLGLVTPLGTGVEKTWKALCAGESGIGRITRF
                     DPTGYDAQIAGEVKDFDPAQFIEKKEIKKMDTFIHYAVGASQLAVDDAKLKVAPEEAT
                     RVGVYIGSGIGGLGSIEHYHDVLREKGPGRVSPFFIPMTIINLASGQVAIRVGAKGPN
                     SCAVTACATGNHCIGDAYRLIQRDDADVMIAGGAEAAITPLGVAGFASAKALSFRNDD
                     PTKASRPFDKDRDGFVLGEGAGVVVLEELEHARARGARIYAEVIGYAMNSDAY"
     gene            complement(829..1062)
                     /gene="acpP"
                     /locus_tag="PK614_07600"
     CDS             complement(829..1062)
                     /gene="acpP"
                     /locus_tag="PK614_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB82112.1"
                     /translation="MATVDERVKKIIAEQLGVEEEEVTLEAHFVEDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKILTVGKALDYIKEKA"
     gene            complement(1162..1905)
                     /gene="fabG"
                     /locus_tag="PK614_07605"
     CDS             complement(1162..1905)
                     /gene="fabG"
                     /locus_tag="PK614_07605"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRB82113.1"
                     /translation="MSLQGKVAIVTGGAQGIGRAIAEALAEDGADIAVADLDPARSQE
                     AVAAIQKLGRRALSVKVNVADWNDAKAMADQVMQEWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLLPMTKQRYGRIVNIASIVGAMGNVGQANYAASKAA
                     VIGFTKTVAREYASRAVTVNAVAPGFIDTAMTQGLSPEVKEALQKQIPLGRLGLPSDI
                     AAAVRFLVSDAASYITGQVLHVNGGMLMV"
     gene            complement(1935..>2758)
                     /locus_tag="PK614_07610"
     CDS             complement(1935..>2758)
                     /locus_tag="PK614_07610"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRB82114.1"
                     /translation="PRSPPNMPRASPLPHPPASTRPAPRPGAPPRYPPPPPAGLKPVA
                     VAGHSLGEYSAVYAAGGVSFRDAVVLVQKRGRYMSEAVPPGTGLVAALLGLSSDVVKA
                     VCQEAASVGVVAAANFNSPGQVVIAGEKAAVERAIEIAKTKGCKKAIPLPVSVPVHTP
                     LMQKAADRLAGEFGGVVWRDLTVPLVNNAEATALQRSDDIRTSLVRQLPSSVRWEESV
                     QTMAKLGVTTFVEIGPGTVLTGLVKRILPGAVTANVNDPKSLEATLTMLGVNSQA"
     assembly_gap    2759..2768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3040..>3437)
                     /locus_tag="PK614_07615"
     CDS             complement(3040..>3437)
                     /locus_tag="PK614_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRB82115.1"
                     /translation="GGTRIPPSEAMVSERSQYIKMKGNETFKVAVRTLEEVARETLAA
                     HHLSVDELDVYIPHQANLRIIKAVADRLNLPLEKVVLNMDRYGNTSAASIPIALDEAV
                     RDGRVKEGQLVMFGAFGAGLTWASALVRW"
BASE COUNT          682 a         1191 c          860 g          694 t
ORIGIN      
        1 tggtacgcgt cgctgttcat ggcgtacccg atcacttcgg catagatgcg ggcgccacgc
       61 gcgcgggcat gctcaagttc ttcaagcacc accacgccgg ccccttcacc caatacgaac
      121 ccgtctcgat ccttgtcgaa cgggcggctg gccttcgtcg ggtcgtcatt tctgaaagag
      181 agggctttcg ccgaggcaaa ccctgccacg ccgagcggcg taatcgccgc ctcagcaccg
      241 ccggcaatca tcacatccgc atcgtcacgc tgaatgagcc gataggcatc gccgatacag
      301 tgattaccgg tggcacaggc cgtcacggcg caggagttcg gccctttggc gccgactcga
      361 atcgccacct gcccggaggc gaggttgatg atcgtcatcg ggatgaaaaa cggagagact
      421 cgcccggggc ccttttccct gagtacgtcg tggtaatgtt cgatcgaccc cagcccgccg
      481 ataccggacc cgatgtagac cccgaccctc gtggcttctt caggcgccac cttgagtttc
      541 gcatcatcca ccgccagctg actcgccccc accgcatagt ggatgaaggt gtccatcttc
      601 ttgatttctt ttttctcgat gaactgagcc gggtcgaaat ccttcacctc tccggcaatc
      661 tgcgcatcat agccggtcgg atcgaatcgc gtgattcggc cgatccccga ctctccggcg
      721 cacagcgctt tccaggtctt ctccaccccg gttccaagcg gagttaccag tccgagaccg
      781 gtcaccacca cacgtctggc aggtcgttcg tgcataccta cggttccgtt atgccttctc
      841 tttaatataa tccagcgctt tgccgacggt gaggatcttc tcggcatcct catcggggat
      901 ctcgatctcg aactcttctt cgagagccat caccaattcg accgtgtcga gcgaatcggc
      961 tcccaaatcc tcgacgaaat gggcctcgag cgtcacttct tcttcctcga cccccaactg
     1021 ctcggcaata attttcttca cccgttcatc tactgttgcc atggacttgc ccacctcctt
     1081 ccccatctta cttgacctcc acattcctca ccgggagatt ccggatcgag gacaccaacc
     1141 cggacacccg acctgactca cttataccat gagcatgcca ccgttcacgt gcaacacttg
     1201 ccccgtgata tacgacgctg catccgacac cagaaaccga accgccgccg caatgtcgga
     1261 gggaagcccc aggcgtccca gcggaatctg cttctgcaac gcttccttga cctccggaga
     1321 cagcccctga gtcatcgccg tatcgataaa gcccggcgcc accgcattca ccgtcaccgc
     1381 acggctcgca tactcacgcg cgaccgtctt ggtaaacccg atcaccgccg ctttggacgc
     1441 cgcataattc gcctgtccca cattacccat cgccccgacg atcgaggcaa tgttgacgat
     1501 ccgcccgtac cgttgtttcg tcatcgggag caggaccgct ttcgtgcaat gaaaggtccc
     1561 gttcaaattg acctgcaaaa ccaggttcca atcttcttcc ttcattctaa gcaacaatcc
     1621 atcacgggta ataccggcgt tgttgaccag aatatcaatc tttccccact cctgcatcac
     1681 ctgatcggcc atggccttgg catcattcca atcggccacg ttcaccttca cactgagcgc
     1741 ccgtcgccct aacttctgga tcgccgccac ggcctcctgc gaacgcgccg gatcgaggtc
     1801 ggcaaccgca atgtctgccc catcttcggc caacgcttct gcaatggccc gtccgattcc
     1861 ctgggctcca ccggtgacaa tcgccacttt tccctgtaat gacatcagcc tgctccccgc
     1921 aatctcaact cgtcttacgc ctgactgttc acgcccagca ttgtgagcgt cgcctccagc
     1981 gacttcggat cattgacgtt ggccgtgaca gcccccggaa gaatcctctt caccaacccc
     2041 gtcagcacgg ttcccggccc gatctcgacg aaggtcgtca cgccgagctt ggccatcgtc
     2101 tgcaccgact cttcccaccg caccgatgac ggcaactgcc gaaccagtga agtccggatg
     2161 tcgtcggatc gttgcagtgc cgttgcttcg gcattattca ccaacggcac ggtcaaatca
     2221 cgccagacca ctccaccgaa ctcccctgcg agtcggtctg ctgccttctg catgagcggc
     2281 gtatggaccg ggacactgac cgggagtgga atcgccttct tacatccctt ggttttggca
     2341 atctcaatgg cccgttcgac ggcggccttt tctccggcga tcacgacttg gccgggagaa
     2401 ttaaaattcg cggccgctac cacgccgacc gaagcggcct cctgacagac cgccttgacc
     2461 acgtccgaag aaagccccag caacgcggcg acgagtccgg ttccaggcgg aaccgcttct
     2521 gacatatagc gtcctcgctt ctgcacgagg accaccgcat ctcgaaacga caccccgcca
     2581 gccgcgtaga ccgccgaata ttcaccgaga ctatgccctg ccacagccac cggcttcaac
     2641 ccggccggcg gtggtggtgg gtagcgcggc ggcgcgccag gccttggcgc cggccgggtt
     2701 gaagccgggg ggtgtggcag gggcgaggct cggggcatat tcggcgggct acgcggggnn
     2761 nnnnnnnnag ccggaccttc aaaacagagc tgggcgacgt cgtatccaag aatcgaggag
     2821 gcctcatcgt agaccgcctt gaccgccgga gacgcctcgt aaaacccgcg ccccattcct
     2881 acagactgcg acccctgtcc ggggaacaaa aacccaattt gtgacgccat catagccgcg
     2941 taaggtatcg tagaaaccac gctcaatgtc aacgggaaaa agctgaacga gccccgcgcg
     3001 tcagtagcga ccgaccgacc ccgaccaggc gtttcccgac taccagcgga ccaatgcgga
     3061 ggcccaggtt agtcccgccc cgaaggcgcc gaacatcacc aactgcccct ctttgacccg
     3121 tccatcccgc accgcttcat ccaaggcaat gggaatcgac gcggcggagg tgttgccgta
     3181 tcgatccata ttcaacacca ctttttccag cggcagattc aggcggtcgg caaccgcttt
     3241 aatgatccgt aagtttgcct gatgggggat atagacatcg agttcatcga ccgacaggtg
     3301 atgcgccgca agtgtctccc tcgccacctc ctcaagagtc cgtaccgcca ccttgaacgt
     3361 ttcattgccc ttcatcttaa tgtactgcga gcgctcgctc accatggcct ctgacggggg
     3421 aatgcgcgtt ccaccac
//
LOCUS       DAORQN010000148         3423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_936_length_3423_cov_27.325119, whole genome shotgun sequence.
ACCESSION   DAORQN010000148 DAORQN010000000
VERSION     DAORQN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_3423_cov_27.325119"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PK614_07620"
     CDS             <1..1299
                     /locus_tag="PK614_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB82116.1"
                     /translation="DVAAWLGASFFLFTILDALIIGEWLIERGQRYIPDIVRQLLIGA
                     EVVAAGVLILWLVMGINLVALVALPTVAAAMVGVALKDTLTRFFSGIELGKVVKVGEW
                     ITVVDREGMVTHIGMEHVTLLTRAQDLVSLPNDLVIQSGVTNFSRPTTTHFCNVYVDA
                     AYRTPPEQVCATLLEAAVAVPGVLQDPKATALVTAFNESAIQYRIKFPIGDFAQRDMI
                     ESRMRTYIWHAFARNGIEIPFPQRVVHQTVEPEVVANGQAVGRMVAQLAAVDFLGTLD
                     AKQLEALAKEARWESYLPGERVVRQGEPGEVLYVIASGKADVRLEQGGLSSTVTTLEA
                     GKFFGEMSLLTGEPRSATVSAATELLVIAVGKQSLLQVVQEDRRLIERIGEVVARRQA
                     ATAAAKAQLSREAAALSAVTHTRSLVERIQNFFWGARKAQ"
     gene            complement(1316..1765)
                     /locus_tag="PK614_07625"
     CDS             complement(1316..1765)
                     /locus_tag="PK614_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRB82117.1"
                     /translation="MWCLTGITMLSMTCFSLAADRQADTLITVKTPTGTLIQAELADT
                     ALKRAQGLMFREHLADDRGMLFIFGDAQPWTFWMKNTKIPLDIIWMDAKKTIVHIERN
                     VPICTRQDDGCPQYHSDEGALYVLELGGGRAAALQLQRGMKLSFKQP"
     gene            1967..2152
                     /locus_tag="PK614_07630"
     CDS             1967..2152
                     /locus_tag="PK614_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82118.1"
                     /translation="MKETRRVVYMRGVFVCPKCRQSYVQEKWIEEFRIRCHKCDYRGT
                     LTQVSVEEHMDEEIVRR"
     gene            2295..2927
                     /locus_tag="PK614_07635"
     CDS             2295..2927
                     /locus_tag="PK614_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRB82119.1"
                     /translation="MIQLWRVLLLCVSSLLAWPIIGRAADSPPAINVLVAYHSLSGHT
                     ERMAEAVAEGARSGPQTHVVLKRVGKVTAEELFAADAIVLGSPVYWSNMAGEVKTFID
                     DWQFKFGVFPEFKLKNKVGAAFATGGQISSGKELTMLSLLAAMLGNQMIVVSGGGAFG
                     ASATTEGDSPGIDAQELTGARELGRRVADVAGAVKMGFESQGNNAGSSGR"
     gene            complement(2947..3153)
                     /locus_tag="PK614_07640"
     CDS             complement(2947..3153)
                     /locus_tag="PK614_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRB82120.1"
                     /translation="MYVCLCKGLTESDVRAAGRQGFLTTRQIVAEFGLRDNGCCGRCA
                     KNIHELVSLAKSQLETTCQNPVRS"
BASE COUNT          649 a          866 c         1100 g          808 t
ORIGIN      
        1 gatgtcgcgg cctggctggg cgcatctttt tttctcttta cgattcttga tgccctgatc
       61 atcggcgaat ggctgatcga acgggggcag cgctatatcc ccgatattgt ccggcagctg
      121 ctcatcggcg cggaagtggt ggctgccgga gtgttgatcc tctggctggt catggggatc
      181 aacctcgtgg ccctggtggc gttgccgacc gttgcggcgg cgatggtcgg tgtcgcgttg
      241 aaagatacgc tgacgcgctt cttttccgga attgagctgg gaaaggttgt gaaggtgggg
      301 gagtggatta cggtggttga tcgtgaaggg atggtgaccc acatcggcat ggagcatgtg
      361 acgctgctga ctcgcgctca ggatctcgtc tcgctgccta acgatctggt gatccagagc
      421 ggggtgacga atttcagcag gcccaccacg acgcatttct gtaacgtgta tgttgatgcc
      481 gcctatcgca ctccgcctga gcaggtctgt gcgacgttgc ttgaggcggc tgtggcggtg
      541 ccgggggtat tgcaggatcc gaaggcgacc gctctcgtca cggcatttaa cgaatcggcg
      601 attcagtatc gaatcaaatt cccgatcggt gatttcgcac agcgggatat gatcgagagc
      661 cggatgcgga cgtacatttg gcatgccttt gcgcgcaatg gaatcgagat tccctttccg
      721 cagcgtgtgg tgcatcagac ggtcgaaccg gaggtcgtgg cgaacgggca ggcggtcggg
      781 cgcatggttg cccaactcgc ggcggtcgac tttttgggta cgctggatgc caagcagctc
      841 gaagcgttgg cgaaagaagc gcgatgggag tcctacttac ctggcgaacg ggttgttcgt
      901 cagggggagc ccggagaggt cctctacgtc atcgcttccg gaaaagctga tgtgcggttg
      961 gaacaggggg ggctcagttc gacggtgaca acgcttgaag cggggaagtt tttcggcgaa
     1021 atgtctctgt taactgggga gccgcgttcg gccacggtat cggccgcgac ggaattgttg
     1081 gtcattgccg tcgggaagca gtcgttgctg caggttgttc aggaggatcg ccgactgatc
     1141 gagcgcatcg gtgaagtggt ggcccgtaga caggcggcga ctgcggcagc gaaagcgcaa
     1201 ctgtctcgtg aggcagcggc cctttcggca gtcacacata cacgttccct cgtcgagcgc
     1261 attcagaact ttttctgggg tgcgcgcaag gcgcagtgat caggttgaag gctggtcagg
     1321 gctgtttgaa gctcagtttc ataccgcgtt gaagctgcag tgccgccgca cggcccccgc
     1381 cgagttccag gacgtacagc gcaccttcat ccgagtggta ctgtggacag ccgtcgtcct
     1441 gtctggtgca gatggggacg tttcgttcga tatggacgat cgttttcttc gcgtccatcc
     1501 agatgatatc gagtgggatc ttggtatttt tcatccagaa tgtccagggc tgggcgtcgc
     1561 cgaagataaa gagcatgccg cggtcatccg cgagatgctc tcgaaacatc aaaccctggg
     1621 cacgtttcaa tgccgtatcg gctaattccg cctggatcag tgtgccggtt ggcgttttga
     1681 ccgtaatgag cgtatcggcc tgccgatcgg cggccaagct gaagcaggtc atactcagca
     1741 tggtgatacc tgtaaggcac cacatcgcaa gtctcagtag tgccggacag tgtgtattat
     1801 caaggcgatc tgttgtgtcg agcgtcacgg ctgcatccta gcgggctcct cgcgggccgt
     1861 caagatactg aacgtgtccg ggggctgagt gtgcgacgct gtgttgcaat ttcgtcggcg
     1921 cctatgccat cctcggccta ttatttgact tggatggagg ctgttcatga aagaaacgcg
     1981 acgtgtggtg tacatgcgag gcgtgtttgt ctgtccgaag tgccgccaat catatgtgca
     2041 ggaaaagtgg attgaggagt tcagaattcg ctgccacaag tgcgactatc gcggaacgtt
     2101 gacccaagtg tccgtcgaag agcacatgga cgaagaaatc gttcgccggt aagcgctacg
     2161 gttctgttgg gcgagttttc cggagatcca gtccccaatc ctcgattcga aaatgcgact
     2221 agttcatgcc aggccgctca tggcctggcc cagccgatgt cagggttgtt ttcttctcca
     2281 gtgagaggtg cgtcatgatt cagctgtggc gagtgctgct tctgtgcgtc agcagtcttc
     2341 tggcctggcc gatcattggc cgagcggctg atagcccgcc ggcgatcaat gtgctggtgg
     2401 cgtatcactc tctttccggg cacaccgagc gaatggccga ggcggttgca gagggcgctc
     2461 gatcgggtcc ccagacgcat gttgtcctga agcgtgtggg gaaagtgacg gctgaggaat
     2521 tattcgccgc cgatgccatt gtgctcgggt ctcctgtcta ttggtccaac atggccggtg
     2581 aggtgaagac gtttattgac gactggcaat tcaagttcgg cgtgttcccg gaattcaaat
     2641 tgaagaacaa ggtaggggct gcatttgcaa ccggcggcca gatttccagt gggaaggaac
     2701 tcacgatgct ctcccttctt gcggccatgc tcgggaatca gatgattgtc gtcagcggtg
     2761 gtggtgcgtt tggcgcttct gcgacaacag aaggcgacag tcctggcatt gatgcgcaag
     2821 agctgactgg agcccgcgag ttgggtcggc gggtggctga cgttgccggt gcggtcaaga
     2881 tgggattcga gtcacaggga aataatgccg gttcgagcgg gcggtgattg cttccaaatc
     2941 gggattctag gaccgcaccg gattttgaca ggtagtctcc agctggctct tcgccagcga
     3001 cacaagttcg tggatattct tcgcgcaacg gccgcagcat ccgttatcgc gcaagccgaa
     3061 ttcagcaacg atttgtctgg tggtcaggaa gccctgacga ccggccgccc gcacatctga
     3121 ttccgttaag cccttacata agcagacata catgagtggc atctcccact gatgatcggt
     3181 tgtccttgcg ggcgtctgac gtacccaatt tttcgatact gataactgtt atcaactatg
     3241 gtgagtatag cggtcaaagg gggacctgtc aacatctgca cagataccct ccatgatggg
     3301 gaagaggtgg ccccggttgg ttggacagcc tattctcttc cttaagtggc gcctcgcggg
     3361 tttttgccta cgcggccgtg agccgtgtcg tcagatggtc agagtacacc tggtcaggcg
     3421 tct
//
LOCUS       DAORQN010000149         3410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_938_length_3410_cov_4.128167, whole genome shotgun sequence.
ACCESSION   DAORQN010000149 DAORQN010000000
VERSION     DAORQN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_3410_cov_4.128167"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PK614_07645"
     CDS             complement(<1..1071)
                     /locus_tag="PK614_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HRB82121.1"
                     /translation="MANENTSVSTTSLVKHPDIMMSVGVVGILMVMLIPLPRFFLDLL
                     LAFNITLSIIILLVGMQVRRPLEFSVFPSILLMVTLLRLALNIASTRLILLHGNEGAA
                     AAGEVIRAFGTFVVGGNYTVGLVVFTILVIINFVVITKGAGRVAEVAARFTLDAMPGK
                     QMSIDADLNAGLINDKEARQRRKEIAQEADFYGAMDGSSKFVRGDAVAAVIITLVNIL
                     GGLTIGVLQQGMTLAAAAQTYTLLTVGEGLVAQVPALIVSTAAGIVITRAASEVNLGF
                     EISRQVLISPKAIGTASGILITLGLVPGLPHLAFLALGGLTAWMAYQINEQQKTTEAV
                     PTPASPQVKAEEAGTQVVPLDLM"
     gene            complement(1094..2170)
                     /gene="flhB"
                     /locus_tag="PK614_07650"
     CDS             complement(1094..2170)
                     /gene="flhB"
                     /locus_tag="PK614_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRB82122.1"
                     /translation="MADSGQNRTEQATPKRKADAKAKGQVALSRDAAMAVGLLGSLGA
                     LYWMTPGILNKLRESLQLWLSKSMEDSTQRTLSLDHLHLILRQIGIDVFIMLGPVIAG
                     IAVVGVGANLMQTGFLWKRDGLQLEFSRISPMGGFSRLFSVRSFSELVKSWLKIFAIG
                     GVGYLTIKQDMLLFSPLTQFGMETLLPTVGWATFKAALMMGAAALVIGGLDYGFQRFE
                     WERDLRMSRDEIKEESRAAEGDPGLKAKIRSTQREMSRKRMMAEVPKADVIITNPTHL
                     AVALKYDSKAMGAPVVVAKGAGFVAEKIREIGRQHGVMIVENKLVARTLFKLVEVGRE
                     VPEDLYHAVAEILVYRVRGKLPPA"
     gene            complement(2163..>2689)
                     /locus_tag="PK614_07655"
     CDS             complement(2163..>2689)
                     /locus_tag="PK614_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRB82123.1"
                     /translation="VRLLFSGFQVAGDMIGTQMGFSAIQMFDPMSHQNTPIIAQFQLT
                     LGSLVFLSLNAHLLVVHAIGMSYEYVPPFGASLSDGIAQDIIHLAQNMLGVALKLAAP
                     VFATLLIVNTVLAILGRAVPQMNVFVTSFPITIGAGFLAMSLAMPFTLLLFEKEFETL
                     VETILGLLKALGHG"
     assembly_gap    2690..2765
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2942..>3108)
                     /locus_tag="PK614_07660"
     CDS             complement(2942..>3108)
                     /locus_tag="PK614_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HRB82124.1"
                     /translation="STFQAMTQINEATLTFVPKVLAVFAATLLFLPWMMGVLIAFMTH
                     IITSIPSLIH"
     assembly_gap    3109..3172
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3253..3396)
                     /locus_tag="PK614_07665"
     CDS             complement(3253..3396)
                     /locus_tag="PK614_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82125.1"
                     /translation="MVVASVLMSMGMMLLPPVMISLPFKLILFVLADGWYLVVGSMVR
                     SFQ"
BASE COUNT          722 a          953 c          957 g          638 t
ORIGIN      
        1 catgagatcc agggggacca cctgggtgcc ggcctcctcg gccttgacct gaggcgaggc
       61 cggtgtcgga acggcttccg tggttttctg ctgttcgttg atctgatacg ccatccaggc
      121 ggtcaggcca cccaacgcga ggaatgcgag atggggaagg cccgggacca ggcccagcgt
      181 gataagaatc cctgaagccg tgccgatcgc cttcggggag atgaggacct ggcggctgat
      241 ctcaaatcct aagttgacct cggaggcggc ccgagtgatg acgataccgg ccgcggttga
      301 aacaatgagc gccggcactt gcgccacgag gccttcaccc accgtcaaca acgtataggt
      361 ctgcgccgct gccgccagcg tcatgccctg ttgcaacaca ccgatcgtca acccgccaag
      421 aatgttgacg agcgtgatga tgacggccgc cacggcgtcg ccgcgcacaa atttgctcga
      481 accgtccatg gcgccgtaga aatccgcttc ctgggcgatc tccttccgcc gttggcgggc
      541 ctctttgtca ttgatcaggc cggcgttcag atcggcgtcg atgctcatct gtttgccggg
      601 catcgcgtcc aaggtgaacc gggctgccac ttcggcgacg cgcccggcgc ccttcgtgat
      661 gaccacgaag ttgatgatga cgagaatggt gaacaccacg agcccgacgg tatagttgcc
      721 gccgaccacg aacgtgccga aggcccggat gacctcccct gcagcggcgg cgccttcatt
      781 gccgtgcagg agaatcaagc gcgtggaggc gatattgagg gcgagacgca ggagtgtcac
      841 catcagcaga atcgagggga acaccgagaa ttccaacgga cggcgtacct gcatgccgac
      901 cagcagaatg atgatggaca gcgtgatgtt gaaggccagg agcagatcca gaaaaaaccg
      961 gggaagagga atcaacatga ccatcaaaat gcccacaacc cccacggaca tcatgatgtc
     1021 ggggtgtttg accaaggagg tcgtggaaac cgatgtgttc tcgttcgcca tctagactcc
     1081 aatccaatcg gatctacgcc ggcggcagtt tgccgcgcac gcgatacacc aggatttcgg
     1141 cgactgcatg atagagatcc tcgggcactt cccggcccac ctcgaccagt ttgaagaggg
     1201 ttcgtgcgac cagcttgttt tcgacgatca tgactccgtg ttgtcgaccg atttcccgga
     1261 tcttctccgc gacaaagccg gcgccttttg cgacgacgac cggcgctccc atcgccttgg
     1321 agtcatattt cagggccacc gccaggtgtg tcgggttggt gataatcacg tcggccttgg
     1381 gtacctctgc catcatgcgt ttccgagaca tctctctctg agtgctgcgg atcttggcct
     1441 tcagccctgg gtctccttct gccgcccggc tttcctcttt gatttcgtcg cgtgacatgc
     1501 ggaggtcacg ctcccattca aaccgttgga agccatagtc gaggccgccg attaccaggg
     1561 ctgctgcgcc catcatgagc gcagctttga atgtcgccca ccctaccgtc ggcaagagcg
     1621 tctccatacc gaattgtgtc agaggagaga acaacagcat atcctgcttg atcgtgagat
     1681 agccgactcc tccgatggcg aagatcttga gccacgattt caccagttcg ctgaaggacc
     1741 ggacggagaa gagccgtgag aacccgccca tcgggctaat gcgggaaaac tccaactgca
     1801 gtccgtctcg cttccagagg aaaccggttt gcatgaggtt ggcacccacg ccgaccaccg
     1861 cgatcccggc aatgaccggg ccgagcatga taaacacatc gatcccgatc tgccttaaga
     1921 tcaagtgcag atggtcgaga ctcagtgtcc gctgtgtgga atcctccatg gatttgctca
     1981 gccacaactg tagcgactct cgaagcttgt tcaggatgcc cggcgtcatc caatagaggg
     2041 cgcccaagct tcccaataac cccacagcca tggcggcatc ccggctgaga gcgacctggc
     2101 ctttcgcttt cgcatccgct ttgcgtttcg gggttgcctg ttctgtccga ttctgtccgc
     2161 tgtcagccat gaccgagtgc cttcaggagt ccgagaatgg tttccaccag ggtttcgaat
     2221 tctttttcaa acagcaacaa ggtgaaaggc atcgccaggc tcatcgcgag gaagcccgcc
     2281 ccgatggtga tcggaaaact cgtgacgaac acgttcatct gaggtaccgc ccgaccaagg
     2341 attgccagga cggtattgac gatcaggaga gtcgcgaaga cgggagccgc gagcttgagg
     2401 gcgaccccca gcatgttctg cgccaggtga atgatatcct gagcgatgcc gtcggataac
     2461 gaggcgccga acggcggcac gtattcgtag ctcatgccga tcgcatgcac caccagcaga
     2521 tgcgcgttga gagagaggaa taccaaggag ccgagcgtga gttgaaattg tgcaatgatc
     2581 ggcgtgttct ggtggctcat gggatcgaac atttgaatag cgctgaagcc catctgcgtg
     2641 ccgatcatgt caccggcgac ctggaagccc gagaacagca gtcgcacggn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnccagg cccagcgtgg cgacgagtcc tgttttgacc ggcattggaa tcgtgcgcgt
     2821 attgaggata ggaaagacgc tgacaatgcc ggcaatccgc accagcagga tggaaaaggc
     2881 ctggaactga ggcagtgcga gatgcagggt ttgcgcagcg ctcatcgtgg taccgggcct
     2941 atcagtgaat cagggacggg atactcgtga tgatatgggt cataaatgca atgaggacac
     3001 ccatcatcca cggcagaaac aagagtgtgg cggcgaacac cgccagcacc ttgggcacaa
     3061 aggtcaacgt cgcttcgttg atctgagtca tcgcctgaaa ggtgctgann nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggcgccg
     3181 acaccagcat ggtggtttca atcgcttggc gaccgatttc tgtgactgtt tcaatcgtca
     3241 tgtgtcgcgc tcctactgaa agctcctgac catcgatccg acgaccagat accagccgtc
     3301 agccagcacg aacaaaatca atttgaacgg caaggagatc atgacgggcg gcaacaacat
     3361 catgcccata gacatcagga cgctggcgac gaccatgtcc acgatgagga
//
LOCUS       DAORQN010000150         3409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_939_length_3409_cov_6.004174, whole genome shotgun sequence.
ACCESSION   DAORQN010000150 DAORQN010000000
VERSION     DAORQN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_3409_cov_6.004174"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PK614_07670"
     CDS             complement(<1..1328)
                     /locus_tag="PK614_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRB82126.1"
                     /translation="MADPFLLSLDESCARFLVGGKAVGLTKVLTAGFPVPKGLCVTTA
                     VYQHGLDQAGVDAVALWTKLLHLSEGQRAQELARIQRLLLEQPWPTGFPADLETRLTG
                     LAGDSSTRWAVRSSATNEDVADMSAAGLYRTTLGVPLAAVLQSIRACWISLWDERVFR
                     YFLRSGADWPCPAMAVVIQPMLSAQAAGVAYSIHPVTGRTSQVSIDAVPGLASSLVNG
                     VVTPDQYVVEVYDDDHRPFRVRRRMLARKRKKLTATPGGVQEESIPEPEQRQSCLADK
                     QLFELARLSKSIEVAFRQPVDLEWVLDVERLWIVQARPITAVRPWPSLTNDECEWTRA
                     NFKETLPELPSPLGLSFLERFMDAYIITPYRRLGCTVPEGLSSVRVFHGRPYLNVTLF
                     YTLVMQLHGNPAFLTEQMGGEPLMFTPSVRPLGALALLRAGVGMLREWRKA"
     gene            complement(1344..2684)
                     /locus_tag="PK614_07675"
     CDS             complement(1344..2684)
                     /locus_tag="PK614_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRB82127.1"
                     /translation="MIQVGVSQLRARALFTTLFRAGLQAVDPYEAVCRQVRFRRGQLT
                     IGSERYPLSQHQRLVVVGAGKASGRMAQALERQLGERIDTGLVVVKYGHGAPTKKIRI
                     LEAGHPVPDEAGLQASRAMMALIGTLKSDDLLIVLLSGGASSLLPSPAPGVTLKAKQL
                     TTKLLLRSGATIHEMNAVRKHLSCVKGGQLAAATSARVASVILSDVIGNDLGTIGSGP
                     TAPDPTTFRDAWRILERYEVARKVPASVRRRLEAGMKKTLPETPKAGAGLFRRVDNLL
                     IGDNQAAVDAVVKAAKGKRLQTLVLSTTVTGEARELAKFFGAIAREIATHGRPLARPC
                     CVIAGGEPTVTIRGQGKGGRAQEFALAAALEIAGLPDVWVAGFATDGTDGPTSVAGAV
                     VDGETTARARRAKLDPPGALQGNDAYPFFKKLRSHIVTGPTGTNVNDLYLLLAL"
     gene            complement(2690..>3409)
                     /locus_tag="PK614_07680"
     CDS             complement(2690..>3409)
                     /locus_tag="PK614_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB82128.1"
                     /translation="FLEDQGESIILAKRTGRCFAPAEKQLAEAGLPDDLKYMLLVESK
                     CIAAAYSRAKASGPWQFINSTGRRYKLHNERWLDERRNLEMSTEAAIKYLRYLRDLYQ
                     GDWFLAMASYNAGEDRVRKLIKEQKINDYWRMHGPRETMRYVARIIAAKEIYSQPEKY
                     LGLTKKDLYPPLETETVTVTIKEPQRRLTAIAEEYGTYFLELKMLNPEFTKDYLPKGT
                     YQLKVPRQTCPNRCFKQEKLP"
BASE COUNT          734 a         1057 c          954 g          664 t
ORIGIN      
        1 gccttacgcc actcccgcag cataccgact ccggcacgaa gaagcgcgag cgccccgaga
       61 ggccggaccg acggggtaaa catcaggggc tcaccaccca tctgttcggt taagaacgcc
      121 ggattcccat gaagctgcat gaccaaggtg taaaacagag tcacgttgag atagggacgt
      181 ccatggaaca cacgaaccga cgagagccct tcggggaccg tgcaaccgag tcggcgatag
      241 ggggtgatga tgtaggcatc catgaatcgc tcgaggaacg acagccctaa cgggctgggc
      301 aattccggca aggtttcttt gaaattagcc cgcgtccact cacattcgtc attggtcaga
      361 gagggccacg gtcgaacggc ggtaatcgga cgggcctgca cgatccagag tcgctccaca
      421 tccaacaccc attcgagatc gaccggttga cgaaacgcga cttcaatgct cttcgacaac
      481 cgtgccagct cgaacagttg cttgtctgcc aggcaggact gccgttgttc cggttcggga
      541 atcgactctt cctgcacacc tccaggggtg gcggtcagct tcttccgttt ccgggcaagc
      601 atgcgtcgtc gtacgcggaa cggccgatga tcgtcatcgt atacctccac cacatactga
      661 tccggcgtca ccactccatt gaccagggaa gaagccaatc ctggcacggc atcgatcgac
      721 acctgagagg ttcgacccgt gacggggtga atagagtacg cgacgcctgc tgcctgtgcg
      781 ctcagcatgg gttgaatgac gactgccatg gcgggacaag gccagtcggc accggaacga
      841 agaaaatacc gaaacacccg ctcgtcccat aacgaaatcc agcaggcgcg gatggattgc
      901 agcacggccg ccaggggcac gcccagggtc gtgcgataca gaccggcggc gctcatatcc
      961 gcaacatctt cattcgtggc ggaggaccgg acggcccagc gtgtcgagga atcgccggcc
     1021 agccctgtca gtcgcgtttc cagatcggcc gggaatcctg tcggccaggg ctgctccagc
     1081 aagagccgct gaattcgtgc cagctcctgt gcccgttgtc cttcagacaa gtggagtagc
     1141 ttcgtccaga gtgccaccgc atcgacacca gcctgatcca ggccgtgttg atagactgca
     1201 gtcgtcacac acagcccctt cggcacagga aagccagcag tgagtacctt cgtcaggccc
     1261 acagccttgc cgccgacaag aaagcgagca cagctctcat ccagagagag gaggaacgga
     1321 tcagccatag agggggatac tagctagagt gcgaggagga ggtaaagatc gttcacattg
     1381 gtcccggtcg ggccagtcac aatatgacta cggagtttct tgaagaacgg ataggcatcg
     1441 ttgccctgaa gcgcaccagg cggatcaagc ttggcgcgcc gggcccgcgc agtcgtttcc
     1501 ccgtctacca ccgcgcctgc aacggaagtc ggtccgtctg taccgtcagt cgcaaaacct
     1561 gccacccaca catcaggcaa cccggcaatc tccaatgccg ccgccaaggc gaactcctgg
     1621 gctcgaccgc cctttccctg ccctcgaatc gtcaccgtag gctctccccc ggcgatgaca
     1681 cagcagggac gagccaaagg gcgaccatgc gtcgcaatct cccgagctat cgcaccgaaa
     1741 aacttggcga gttcgcgagc ttcccccgtc acggtagtgg aaaggacaag cgtctgtagc
     1801 cgcttcccct tcgcggcctt cacgacggca tcgacagccg cttgattgtc tccaatgagc
     1861 agattgtcca cacggcgaaa gagtccggcg ccggctttgg gagtttcggg cagggtcttc
     1921 ttcatgcccg cctccaggcg tcgacgcacc gaagctggaa ctttccgggc aacctcgtat
     1981 cgctccaaaa ttctccaagc gtcccgaaat gttgtggggt caggagcggt cggaccagac
     2041 ccgatcgtac caagatcgtt cccgatcaca tccgagagga tcacactcgc cacacgcgcg
     2101 gaagtcgctg cggcaagctg cccgcctttt acacaagaca agtgctttcg aaccgcattc
     2161 atttcgtgaa tggtcgctcc agaccgcaac agcagcttag tcgtcagctg cttggccttg
     2221 agggtgaccc caggagcggg ggagggaagg agactcgagg caccgccgga caagaggaca
     2281 atcagcaggt cgtccgattt cagcgtcccg attaatgcca tcatcgcacg gctggcctga
     2341 agccccgcct cgtccggaac cggatgcccg gcttccagga tgcgaatttt cttcgtcgga
     2401 gcaccatgcc cgtatttcac cacgaccagt ccggtgtcga tcctctcccc cagctgccgc
     2461 tccaacgcct gagccatgcg acccgacgcc ttcccggccc cgaccactac aagtcgctga
     2521 tgttgtgaaa gaggatagcg ctccgaaccg atagtgagtt gcccgcgccg gaagcggacc
     2581 tgacggcaca ccgcctcgta ggggtcgacg gcctgcagtc ccgcacgaaa cagtgttgtg
     2641 aagagggctc gtgccctgag ctgcgagact ccgacttgta tcatgggcac tacgggagct
     2701 tctcttgctt gaaacagcga ttcggacagg tttgtcgcgg caccttaagc tggtaggtac
     2761 cctttggcaa gtagtccttc gtgaattcgg gattcagcat cttcaattca agaaaatagg
     2821 ttccgtattc ctcggcgatc gccgtgaggc ggcgttgcgg ctccttgatg gtaaccgtca
     2881 ccgtttctgt ttcaagggga gggtagagat ccttcttggt cagaccaaga tacttctccg
     2941 gctgggaata gatctctttc gccgcaataa tgcgtgccac ataccgcatg gtctctcgtg
     3001 ggccgtgcat gcgccagtag tcattgatct tctgttcttt gatcaatttc cgcacgcggt
     3061 cttccccggc gttgtaggac gccatcgcca agaaccaatc tccctggtac agatcccgca
     3121 gataccgtag atacttgatg gcggcttcgg tggacatttc aagattgcgg cgttcatcca
     3181 gccagcgttc gttatggagc ttataccgtc gccctgtgga attaatgaac tgccagggac
     3241 ccgatgcctt ggcccgggaa taggcggccg ctatacactt gctctcgacc aacaacatat
     3301 atttcaagtc atccggcaat ccggcctcag ccaactgctt ttctgccggc gcaaaacatc
     3361 gacctgtccg tttcgccaga atgatacttt caccttgatc ctcgaggaa
//
LOCUS       DAORQN010000151         3395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_942_length_3395_cov_5.329042, whole genome shotgun sequence.
ACCESSION   DAORQN010000151 DAORQN010000000
VERSION     DAORQN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_3395_cov_5.329042"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK614_07685"
     CDS             complement(<1..185)
                     /locus_tag="PK614_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRB82129.1"
                     /translation="MWVFLNDRFVRKEEALVSVFDHGFLYGDGVYETLRSYGTRIFMR
                     DQHLARLRRSAEAIGLD"
     gene            complement(185..1669)
                     /locus_tag="PK614_07690"
     CDS             complement(185..1669)
                     /locus_tag="PK614_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRB82130.1"
                     /translation="MSFSSHQAFLSGPPQPLVVVRPQPDVDAFELYCRLAPAERPSFL
                     LESANGTDTTARYSFFGRDPYLTLTSRAQEYQIETGGQIRRYQGSGIQALQQALAEST
                     ITKPEGLPPFYGGAVGYLSYDLVRSFELLPTLAADDLHLPELHMAFFDVVAAIDHHTR
                     QLYLMYCPPLSRFQAEPREKLYREGCDRLAELEARLTSPVAPLTSSPWPTQTTFVPSQ
                     SREAYKTRVRRCQDYIAAGDIYQANLSHRFTLDLHAAASPTGFEGYSTELYRRLRRIN
                     PAPFAGLVRFADLSLVSSSPERLVRLTGSQASTRPIAGTRPRGTGLQQDQLLRAELLA
                     NPKERAEHVMLVDLERNDLGKVCRYGSVRVDEFMTIEQYSHVSHLVSDVVGTLQPGVS
                     PLDLVKAVFPGGTITGVPKLRCMEIIEELEPVRRGLYTGALGYFSWNGDLDLNILIRT
                     LVLTKNRGYLQVGAGIVADSDPDREYDETLAKAGAFFKILEAGR"
     gene            1845..2063
                     /locus_tag="PK614_07695"
     CDS             1845..2063
                     /locus_tag="PK614_07695"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRB82131.1"
                     /translation="MPPSQDPLYAGLGQAVRIGTELLAALIVGGGLGWVVDTYLLDSN
                     PWGLVVGLGLGAAAGVRSAYRSAQRWQS"
     gene            2094..2843
                     /locus_tag="PK614_07700"
     CDS             2094..2843
                     /locus_tag="PK614_07700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRB82132.1"
                     /translation="MEESPLHAFELHDLIPLVPAGVDISINKAVILMWVIVGLVAFLM
                     ISAAAARKLVPGKLQNMAELIVEFIRGIILDTMGEPGMKYFPLIATLFLFILFANLIG
                     LIPGSYTVTSQIIVTAVFAVGVYGLSIVLGFSLHGMKFLGILVPPGTPGWLLPLMIPI
                     ELISQLARPISLAVRLFANMTAGHVILGVLFGLAISGGLLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLSCVYLGDAVTLHGHSEHAH"
     gene            2880..3110
                     /gene="atpE"
                     /locus_tag="PK614_07705"
     CDS             2880..3110
                     /gene="atpE"
                     /locus_tag="PK614_07705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRB82133.1"
                     /translation="MDAAAAALVGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            3185..>3395
                     /locus_tag="PK614_07710"
     CDS             3185..>3395
                     /locus_tag="PK614_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRB82134.1"
                     /translation="MPQFESHFFSSLIFWEILSFGILFFLLYKYAFPSLLGMLEEREK
                     KIKDSLDQAERHRSEAERRLKEYEAK"
BASE COUNT          657 a          879 c         1097 g          762 t
ORIGIN      
        1 atgtccaggc cgatggcttc ggcggagcgc cggagccggg ccaggtgttg gtcgcgcatg
       61 aagatgcggg ttccatagga gcgaagggtt tcgtagactc catcaccgta gagaaaccca
      121 tggtcgaaca cggagacgag ggcttcttct ttgcgtacaa aacgatcatt gagaaacacc
      181 cacatcagcg gccagcctcc agtattttga aaaatgcgcc cgctttggcg agcgtttcat
      241 catattcgcg gtcgggatcc gagtcggcca caatgccggc accgacttgc agataccctc
      301 ggttcttggt cagcacgagc gtacgaatga gaatgttcag gtccaggtcg ccgttccaac
      361 tgaaataacc caacgccccc gtgtagagtc cgcgccgcac cggctccagt tcttcgatga
      421 tttccatgca acggagttta gggacaccgg tgatggttcc gccggggaac acggctttca
      481 caaggtcgag gggtgagacg cccggttgca gggtgccgac cacgtctgac accaggtggc
      541 tgacatgcga atactgttcg atggtcatga actcatcgac ccgcaccgag ccgtagcggc
      601 agacctttcc gaggtcgttg cgttcgagat ccaccagcat cacatgttcg gcgcgttcct
      661 taggattggc aagcaactcc gcgcgcagaa gctggtcctg ttgcaaaccg gtgcctcgcg
      721 gcctggtgcc ggcgatgggt ctggtgctgg cctgcgagcc ggtcagccgc acgagccgct
      781 caggggagct gctgacgagg ctgaggtcgg cgaatcggac gagccccgcg aacggcgcag
      841 ggttgatccg gcgaaggcgg cgatagagct cggttgagta tccctcgaat ccggtcggtg
      901 aagcggcggc gtgcagatcg agggtgaagc ggtgcgacag attggcctga tagatatcac
      961 cggcggcgat atagtcctgg caacgccgca cacgagtttt gtaggcctcg cgcgattggc
     1021 tgggcacaaa ggtggtttga gtgggccagg gcgaggaggt aagaggcgcg accgggctcg
     1081 tgaggcgggc ttccagctca gccagccggt cacagccttc gcgatagagt ttctcccgcg
     1141 gctccgcctg aaatcgggac agaggggggc aatacatcag gtagagctgt cgcgtatggt
     1201 gatcgatcgc ggccacgacg tcgaaaaagg ccatgtgcag ttcagggagg tggagatcgt
     1261 cggcggctag ggtcggaagt aattcgaacg aacgaacgag gtcgtagctg aggtatccga
     1321 ctgcgccgcc ataaaacggc gggaggcctt ccggtttggt aatggtcgat tcggcgagcg
     1381 cctgttggag ggcctgaatg ccggaacctt gatagcgccg gatctgtccc ccggtctcga
     1441 tctggtattc ctgcgcgcgg cttgtcaggg tgagataggg gtcgcgtccg aaaaatgaat
     1501 agcgcgcggt ggtgtcggtg ccgttggcgc tttccaggag aaacgaggga cgttcggccg
     1561 gggccagccg acagtacaac tcgaacgcgt cgacatccgg ttgtggacgc acgaccacca
     1621 gcggttgcgg cggtccgctc aggaatgcct ggtgagagga aaagctcatg aacctgcgtt
     1681 tcagaggggt gccatacccg agcaaatgac tataccgcat cgagcggccg ggtcataggt
     1741 ggccaacttc ttgacaagcc cccaacgaat ttgcttgaat ggcgcgcccg ccgatgggtg
     1801 cactctctcc gattgcgctg aggagtgccg cacgtgagca tctgatgccc ccctctcagg
     1861 atccgttata tgcggggctc gggcaggctg tccggatcgg gacggaactg cttgctgcgt
     1921 tgatcgtcgg gggaggactg ggttgggtcg tcgacaccta tctgctcgat tctaatccat
     1981 ggggattggt ggtggggtta gggttggggg ccgcagccgg tgtacggagc gcctatcggt
     2041 ctgcgcaacg atggcagagt taaccagaat cgtctgactg ataaaggatc atcgtggaag
     2101 aaagtccgtt acatgcgttt gaactgcacg atctgattcc cttggttccg gccggggtgg
     2161 atatttccat caacaaggcc gtgattctga tgtgggtgat cgtcggcctg gtggcgttcc
     2221 tgatgatctc ggctgcggcg gcccgtaagt tggttccggg caaattgcag aacatggccg
     2281 aattaatcgt cgagttcatt cgcgggatta ttttggacac catgggcgag ccggggatga
     2341 aatatttccc cctgatcgcc acgttgttcc tctttattct ctttgccaat ttgatcgggt
     2401 tgattcccgg ttcctatacc gtcaccagtc agatcattgt gacggcagtc tttgctgtag
     2461 gggtttacgg gctgagtatt gtgctcggct tttcccttca tggcatgaag tttctcggca
     2521 ttctcgtgcc gcccgggaca ccgggttggc tcttgccgtt gatgattccg attgaattga
     2581 tcagtcagtt ggcgcgaccc atttccctgg ccgtccgtct gttcgcgaat atgactgccg
     2641 gccacgtcat tctgggagtc ttgttcggtc tggcgatcag cggtgggttg ctgatcgggt
     2701 ggctgccatt cgcgttcacc atcgccatga acggactcga agttggtatc gcgtttattc
     2761 aagcctatat ttttaccgtg ctgagctgtg tgtatctggg tgatgcggtt accctgcacg
     2821 gtcatagcga gcacgcacac taggtcgagt tgtttacata agggaggaga ggacaaacaa
     2881 tggatgcagc agcagcagcg ttggtgggta tgggattggc ggcggcagga tttgccggcg
     2941 ccggtgtggg catcgggtat atcttcggga aaatgatcga ggccgtggcc cggcagccgg
     3001 aagcggaagg ccgcgtcggc aagtacatgt ggatcgggtt cgcgttggtc gaagccattg
     3061 cactgtacgg gctggtcatt gcgttcatca tcatgggctt gcgtaagtag tcgtttcgac
     3121 ggctgagccc tgatgctggg gcctattccc ggctcagaac ctcaactcat ttgggtaata
     3181 gactatgcct caatttgaat cacatttctt ttcgtccttg atcttctggg agattctctc
     3241 ctttgggatc ctgttctttc tgctctacaa gtatgcattt cccagcctgt tgggcatgct
     3301 ggaagagcgc gagaaaaaga ttaaggacag tctcgatcag gcggaacgcc accgttccga
     3361 agccgagcgc aggctgaagg aatacgaagc caagc
//
LOCUS       DAORQN010000152         3385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_945_length_3385_cov_6.932132, whole genome shotgun sequence.
ACCESSION   DAORQN010000152 DAORQN010000000
VERSION     DAORQN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_3385_cov_6.932132"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PK614_07715"
     CDS             complement(<1..517)
                     /locus_tag="PK614_07715"
                     /inference="COORDINATES: protein motif:HMM:NF037404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiV family abortive infection protein"
                     /protein_id="HRB82135.1"
                     /translation="MRIKAIQDLSQLTDDALFSEIAAGASLCLSNAQQIDGDSFLLND
                     LKRPAGSQILRLAAEEEAAKVLILLDAVRCPRIERQPDFNRQVQYFNDHLAKGIYAQY
                     CWRRPHSFSAVREWVDRERKEYYLDGPNNVDWIFYNEILRQREETIYVDYVENDGAHY
                     WHDPRCTDELRL"
     gene            complement(979..1752)
                     /locus_tag="PK614_07720"
     CDS             complement(979..1752)
                     /locus_tag="PK614_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HRB82136.1"
                     /translation="MVDRSAYLARIGYQGSVVPSMETLRGLHLSHVLTVPFENLDIHL
                     GRPISLDPSDLFQKIVRGRRGGYCFELNGLFALLLEELGFVVTRLAARVLYGTKGIRP
                     RSHQVLLVHLDGERWIVDVGFGGQGLREPLPFVAGLEHRQGPDQFRLTTDERGEHVLQ
                     CDVEGAWANLYSFTLEPWLPVDYQFANYYHSHAPDSPFVQRWMCTMPTLNGRKIFTGK
                     LLKVRGLDGTQELHVTNEGECKQLLQEHFWLTINDHLKS"
     gene            1895..>3385
                     /locus_tag="PK614_07725"
     CDS             1895..>3385
                     /locus_tag="PK614_07725"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82137.1"
                     /translation="MAHRSPADRTTNDLIVEGARQNNLKNISLRIPHNRVTAITGLSG
                     SGKSSLAFDTLFAEGQWRYVESLSTYARMFLDKVNRPDVDRITNIRPAIAIEQKNPIR
                     TARSTVGTATELADLLRLLFAKIGKPVCPDCKQEARGYHPGSVAEELLARFPDARAMV
                     LFPLKDLGPGHDRSLLDSLLKRGFTRLRCGEELLDLHEQAVLPETRESGIQVVLDRLV
                     LRPDNRHRLIEAIEVAFQEAEGTCQVLVIGQGLRTYSTHFRCQGCGRTFEPLRPLLFS
                     FNHPLGACPECKGFGNILQYDKDLVIPDRSKSLANGVIEPWSKPGSDWWQKQILLAMK
                     KQGVDLTTPFQELPEEVQQLIWEGSDHVEGVRQYFDYLETKRYKLHVRVLLSRYRSPA
                     SCPTCHGSRLKPSARFVKLAGQDIVEIGELTIEAAAAWFERLALPAFDAEIAKDILRQ
                     LHAKLNFLLRVGLSYLTLSRQTKTLSGGEAQRIALANQLGSRLVGTL"
BASE COUNT          743 a         1044 c          935 g          663 t
ORIGIN      
        1 taagcctcag ctcgtccgtg cacctggggt catgccagta gtgggctccg tcattttcga
       61 cataatctac gtatatggtt tcttctcgtt ggcggagaat ctcgttgtag aagatccagt
      121 caacattatt cggcccatca aggtagtact cctttcgttc tcgatcgacc cattcacgaa
      181 ccgccgaaaa tgagtgcggg cggcgccagc aatattgtgc atatatgccc ttcgctaagt
      241 gatcattgaa gtactgcacc tgtctgttaa agtcgggctg ccgctcaatg cgagggcagc
      301 ggacagcgtc gagcaagatc agaactttag ccgcctcttc ctcggctgcg aggcgcagaa
      361 tctgactccc cgcaggccgc ttgagatcat tcagcaagaa tgagtcgccg tcgatttgct
      421 gcgcatttga taggcagagc gaggcaccgg ctgcaatctc agagaagagt gcgtcatcgg
      481 tcaactgcga aagatcttga atggccttta tacgcatcgg tgcccaaaag gacgcccaaa
      541 gtgccagcga accgagacgc cacttagtga tacacccctg ctgagcgtcg tattagacac
      601 gactgatctt tctcggcact ggcaccgctc gcttgcggtt ccaccgactt ccaagcccag
      661 cgggcgcagg cttgcctcga tgcgggggat tcttgaataa tcgatgaatg aaatcaaggc
      721 aggagggacc ggcacatgca cgcgggcgca ccgtgataat ccgttccgtc atgttgctcc
      781 tactgctcgg ctcaatcagt ggatgttcga tcctgtttca gccgctgagg actccgatcg
      841 gacattcctc agatgagaat ggccggcgtt gctcgaccta tcagaccagg tcggagggtg
      901 tgtacgaaac aatctgcgag cacggcggct cggatcaagg tcccatataa ggcccgtgga
      961 gctcagagag gtaacgattc aagacttcaa gtgatcgtta atggtcagcc agaagtgctc
     1021 ctggagcagc tgtttgcact ccccttcatt cgttacatga agttcctgcg tgccatccag
     1081 ccctcgaact ttgagcagtt taccagtgaa tatcttccgc ccattcaggg tgggcatcgt
     1141 gcacatccag cgctgcacga agggcgaatc aggtgcgtgc gagtggtaat agttggcaaa
     1201 ctggtagtcc accggcagcc agggttcgag cgtgaaggaa tagagattcg cccaagcccc
     1261 ctcaacgtca cattgcagca catgctctcc ccgctcgtcg gtcgtgagcc taaattgatc
     1321 cggcccttgc cgatgttcca gaccagccac aaagggcaac ggctcgcgca atccctgccc
     1381 tccgaatccc acatccacaa tccagcgctc tccgtccaga tgaacgagca gcacctggtg
     1441 actccgcggc cttatgcctt tcgtcccata caacacccgg gcagccagtc gcgtaacgac
     1501 aaaaccaagc tcctccaaca ggagcgcaaa caatccgttc aactcgaagc aatacccgcc
     1561 ccggcggccg cgcacgatct tttgaaacag atcagaggga tctaacgaaa tcgggcgccc
     1621 gaggtggatg tccagatttt cgaacggcac ggtcaggaca tgggagagat gtaagccacg
     1681 caacgtttcc atcgacggaa ccaccgagcc ttgatatccg atacgcgcga gataggccga
     1741 tcgatcaacc atagagtcct ccgatgagtg ggccattata cgaatctgcc cggacggatc
     1801 acacaggtct cagcgccgac gccggcaggt gacattgcag acgactgcgg cgcttgctac
     1861 gatggtcggt ctttcgccgg aaggaaccgc cgctgtggca caccgtagtc cagcagatcg
     1921 caccacgaac gacttgatcg tcgaaggtgc gcgccagaat aatctgaaga acatctccct
     1981 gcgtatccct cacaaccggg tcactgccat caccggcctg tccggatccg gcaaatcgtc
     2041 cctcgctttc gacacactgt ttgccgaagg acagtggcgc tatgtggaat cgctctcgac
     2101 ctacgcgcgg atgtttctcg ataaggtgaa ccggcccgac gtcgatcgga tcaccaacat
     2161 ccgccccgcg atcgcgatcg aacagaaaaa tccgatccgc accgctcgtt cgaccgtcgg
     2221 caccgccacc gagctcgccg acctgctgcg tttgctgttc gcgaaaatcg gcaaacccgt
     2281 ctgcccggat tgtaaacaag aggcgcgtgg ttaccatccg gggtcggtcg ccgaggaact
     2341 gctggcacga ttccccgacg cgcgggccat ggtgctcttt cccctcaagg atctcggccc
     2401 gggccacgac cgctcgctgc tcgactcact cctgaagcga ggattcaccc ggctgcgatg
     2461 cggcgaggaa ctcctggatc ttcacgaaca ggccgtgctt ccggagaccc gcgagtcggg
     2521 catccaggtc gtgctggatc gactcgtcct caggccggat aaccgccacc ggttgatcga
     2581 ggccatcgag gtggcgtttc aggaagccga gggcacctgc caggtcctgg tgatcggaca
     2641 ggggctccgg acctacagca cacactttcg ctgccagggg tgcgggcgga ccttcgaacc
     2701 actacgcccg ctgctgttct ccttcaacca cccactcgga gcctgcccgg agtgcaaagg
     2761 gttcggcaat atcctgcaat acgacaagga tctggtcatc cccgaccgca gcaaatcgct
     2821 cgccaacggc gtcatcgagc cctggagcaa gccgggctcg gactggtggc agaagcaaat
     2881 attgctggcg atgaagaaac agggcgtgga tttgacgacc ccgtttcaag agcttcccga
     2941 agaggtgcaa cagctgatct gggaagggag cgatcacgtt gaaggagtcc ggcaatactt
     3001 cgactatctg gaaaccaaac gctataaact tcatgtgcgg gtgctgctca gccgctaccg
     3061 cagtccggcc agctgcccga cctgccacgg cagccgactc aaaccgtccg cccgtttcgt
     3121 caaattggca gggcaggaca tcgtggagat cggtgaactc accattgaag ccgccgccgc
     3181 ctggttcgag cgcctggccc tgcccgcctt cgacgcggaa atcgccaagg atatccttcg
     3241 ccaactgcat gcgaagctga actttctact ccgcgtagga ttgagctacc tcacgctgtc
     3301 gcggcagacc aaaaccctct ccggcgggga agcacaacgg atcgcgttgg ccaaccagct
     3361 gggctctcgc ttagtgggca cgctc
//
LOCUS       DAORQN010000153         3378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_949_length_3378_cov_7.423112, whole genome shotgun sequence.
ACCESSION   DAORQN010000153 DAORQN010000000
VERSION     DAORQN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_3378_cov_7.423112"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK614_07730"
     CDS             complement(<1..694)
                     /locus_tag="PK614_07730"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRB82138.1"
                     /translation="MLQTSEKIWMDGKFVAWADAQVHVLTHSLHYGLAAFEGIRCYKG
                     KSGSAIFRLPEHVDRLFESAHIGLIEMPFDRKQISEAIVETVRVNRLEACYIRPLVYI
                     GYGAMGLYPAENPIKVSIAAWKWGTYLGDEALSKGIRARVSSFTRHHVNVSMTRGKIS
                     GYYVNSILAKREVKADGYDEAIMLDPEGYVAEGTGENVFIVRRGVLKTTPLTSILEGI
                     TRNSIIQLAKERN"
     gene            904..1218
                     /gene="rplU"
                     /locus_tag="PK614_07735"
     CDS             904..1218
                     /gene="rplU"
                     /locus_tag="PK614_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRB82139.1"
                     /translation="MYAIIETGGKQYRVETGTVLQVESLPGDVGHSVQIDKVKLLHGE
                     GGLVVGQPVVAGATVTAEIIRQGRTRSITVFKKHRRKNYRRTRGHRQGFTQLRVTGIE
                     TK"
     gene            1240..1503
                     /gene="rpmA"
                     /locus_tag="PK614_07740"
     CDS             1240..1503
                     /gene="rpmA"
                     /locus_tag="PK614_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRB82140.1"
                     /translation="MATNKGGGSTRNGRDSNPQYLGVKAYGGETIKAGSIIVRQRGTK
                     FFPGLNVGLGRDHTLYAYVSGVVKFEGGRGRQKVSVYPVTAKA"
     gene            1629..2660
                     /gene="obgE"
                     /locus_tag="PK614_07745"
     CDS             1629..2660
                     /gene="obgE"
                     /locus_tag="PK614_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRB82141.1"
                     /translation="MSTFVDQVQILVKAGHGGNGACSFRREKFVPRGGPDGGDGGDGG
                     SVVVEATTRLSTLLDLRYQKHYEAEKGETGGGSNCHGRRGADVRIPVPVGTMVFDENT
                     QELLADLTKDGETCVIAKGGQGGRGNTQFATSTNRVPTQFEPGTPGEERLIRLDLKLL
                     ADVGLVGYPNAGKSTLIAAVSAARPKIADYPFTTLTPNLGVVRWTGEQTFVIADIPGL
                     IEGAHEGKGLGFQFLRHIERTSLLLHVIDISEWATEDPVASLEIMRHELTAYDDTLAA
                     RPFAVVGTKLDVKGEGERLERLRKYCQRRKIPFFAISAATREGLDECIRYIGQQVELL
                     RKTPCETNS"
     gene            2642..>3378
                     /gene="proB"
                     /locus_tag="PK614_07750"
     CDS             2642..>3378
                     /gene="proB"
                     /locus_tag="PK614_07750"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRB82142.1"
                     /translation="MRDELLRQAKRVVIKIGSSLIASRETGLSAERIERLAGEIAEVR
                     AQGREVLVVSSGAVVSGIKKLGLREYPKSLPLKQAAAAVGQSLLMWAYEKAFERLTLR
                     VAQVLLTHEDLADRRRFLNARHTLTTLIKFGVIPIINENDTVAVEEIRVGDNDTLAAQ
                     VAHLVDADLLIILSDVDGLFTVDPRKDPTAALIPLIPEITEDIEQRAGMSSTFEGTGG
                     MATKVRAAKKVGEYGVATLILNGTQTRL"
BASE COUNT          775 a         1031 c          938 g          634 t
ORIGIN      
        1 tattacgctc cttcgccaat tggatgatgg agttgcgcgt aattccctca agaatggagg
       61 tcaatggagt cgtttttaag acaccacgtc gcacgatgaa cacattttct cccgtgcctt
      121 ccgctacata cccttcagga tccagcatga tcgcttcgtc atagccatcg gccttcactt
      181 cccgtttggc cagaatggag ttgacgtaat agccggaaat tttcccgcgc gtcatggaaa
      241 cattcacatg gtgacgcgta aaggacgaca ctctggctcg aatccccttg gacagggcct
      301 catcgcccag ataggtcccc catttccaag ctgcgatgct caccttgatt ggattctcag
      361 cagggtagag ccccatggca ccatacccga tgtacaccaa cggacgaatg taacaagcct
      421 ccaaccgatt gacccggacg gtttcgacaa tcgcttccga gatttgcttc ctgtcgaaag
      481 gcatttcgat caacccgatg tgggcggatt caaagagacg atccacgtgc tcaggtagcc
      541 tgaaaatggc tgacccgctc ttccccttgt agcagcgaat accctcaaac gctgctaacc
      601 cgtagtgaag cgagtgcgtg agcacatgaa cctgcgcatc agcccaggcc acaaacttcc
      661 catccatcca tattttttcg ctggtttgta acatgcgcgg gataatacca gcgcctcgcg
      721 acgaccgtca agacaaccgc acgtagacat ttcgatgcag agtcttcagg aattccgccg
      781 ctcccgccct ccttcacaca gcggtgcctg aagccagctt cgttcaatga cgcactaatt
      841 cttgacacct tctccgccgc tatgataaac acccacgttc tgctgacgag gagaaagacg
      901 cacatgtacg ctattatcga gacaggcgga aaacagtatc gagtggaaac cggaaccgta
      961 ctccaggtgg agtcacttcc gggtgatgtc gggcactctg tgcagatcga caaggtcaag
     1021 ctgctgcatg gcgagggcgg actggtagtc ggccagccgg tggtggcggg ggcgaccgtt
     1081 acagcggaaa tcatccgcca gggtcgcacc agatcgatca cggtcttcaa gaagcaccgt
     1141 cgcaagaatt atcgccgtac cagaggccac cggcagggct ttacgcagct gcgggtcact
     1201 gggatcgaaa cgaagtaacg actcgaaggg gagtttgcca tggcaacaaa caaaggcggc
     1261 ggttcgacaa ggaacggccg tgacagtaac ccacaatacc tcggtgtcaa agcctacggc
     1321 ggagagacga tcaaggcagg ctccatcatt gtgcgccagc ggggaacgaa gtttttccca
     1381 ggcctcaacg tcggtttggg ccgggatcac accctctatg cctatgtgtc cggtgtcgtg
     1441 aagttcgaag gtggccgcgg ccgtcagaag gtcagcgtct atcccgtcac agcgaaggcc
     1501 tgatccctcc tcagtccata cctttgttcc tacggaacag gtgccttccg gtacagcatc
     1561 ccccatgagc cggcgaatac gctatactgc tgtttctgaa taaccacgcc ggctcgggga
     1621 tcgctgccat gtctaccttt gtcgatcaag tccaaatcct ggtgaaggcc ggtcacggcg
     1681 gcaacggcgc ctgcagcttt cgacgggaaa agttcgtccc tcgcggcggc cctgacggcg
     1741 gtgacggcgg tgacggcggc agcgtcgtgg ttgaagccac cactcgcctc agcaccctgc
     1801 tggatcttcg ttaccaaaaa cattacgagg ccgaaaaagg cgagaccgga ggaggcagca
     1861 actgccatgg acgccggggc gccgatgtgc gcattcccgt cccggtcggc accatggtgt
     1921 tcgacgaaaa cacccaggaa ctgctggccg atcttacgaa agacggagaa acctgcgtca
     1981 ttgccaaggg cggtcaggga ggccgaggca acacccaatt cgccacatcg accaaccggg
     2041 tgccgacaca attcgagcca ggcactcccg gcgaagagcg attgattcga ctcgatctca
     2101 agctcctggc agatgtcgga ttggtcggct atccgaacgc cggaaaatcg acactcatcg
     2161 ccgccgtgtc cgccgctcgc cccaaaattg ccgattaccc cttcaccacc ctgacgccca
     2221 acctgggcgt ggtacggtgg accggcgagc agacctttgt catcgccgat attccaggat
     2281 tgatcgaagg ggcacatgaa ggcaaggggc tgggatttca gtttctgcgc cacatcgagc
     2341 gaaccagcct gctactccat gtgatcgaca tttcagaatg ggccaccgag gacccggtcg
     2401 cgagcctgga gatcatgcgc cacgaactga ccgcctacga tgacacattg gcggcgcgcc
     2461 cctttgccgt cgtgggcaca aaactcgacg tcaaaggcga gggggaacgg ctcgaacggc
     2521 tccggaagta ctgtcagcga cgcaagattc cattttttgc gatttcggca gccacgcgag
     2581 aaggactgga cgagtgcata cgctacatag gccagcaagt ggaactgctt cggaaaaccc
     2641 cgtgcgagac gaactcctaa gacaagccaa acgcgtcgtc atcaagatcg ggagcagcct
     2701 gatcgcctcg cgcgaaaccg gcctgagcgc cgagcggatc gagcgccttg ccggcgaaat
     2761 cgccgaggtg cgggcacagg ggcgcgaggt actggtcgtc tcatccggcg cggtcgtatc
     2821 cggcatcaaa aaactcgggt tgcgcgaata cccgaaaagc ctgccgctca aacaggctgc
     2881 tgctgccgtc ggccaaagcc tcctcatgtg ggcctacgaa aaggcctttg agcgcctcac
     2941 gctccgcgtc gcacaggtgc ttctcacgca cgaagacctc gccgatcgac gacggtttct
     3001 caacgcccgg cacaccctca ccaccttgat caagttcggg gtgattccga tcatcaacga
     3061 aaacgatacc gtcgcggtcg aggaaatccg cgtgggggat aacgatactc tggcggcgca
     3121 agtcgcccac ctcgtcgatg cggatttact gatcatcctc tcggacgtcg acgggctctt
     3181 taccgtggac ccccgcaagg acccgacggc cgccttgatt ccactaatcc ccgagattac
     3241 ggaagacatc gaacagcggg ccggcatgtc gtccaccttc gaaggcacag gcggcatggc
     3301 caccaaagta cgcgctgcca agaaggtggg ggaatacggc gtcgccaccc tgattctaaa
     3361 tggcacccag acaagact
//
LOCUS       DAORQN010000154         3362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_954_length_3362_cov_5.572120, whole genome shotgun sequence.
ACCESSION   DAORQN010000154 DAORQN010000000
VERSION     DAORQN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_3362_cov_5.572120"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK614_07755"
     CDS             complement(<1..354)
                     /locus_tag="PK614_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82143.1"
                     /translation="MIEWVGRKAIAWYTYLAALATLFTQAVLDLVLPTRQGRGETLRV
                     LVRQILTASGGPLWGFSREQLQDVNPERALQHPNWKMGSKITIDSATLMNKGLEVIEA
                     RWLFDIPETQIEVLVH"
     gene            complement(574..906)
                     /locus_tag="PK614_07760"
     CDS             complement(574..906)
                     /locus_tag="PK614_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB82144.1"
                     /translation="MEQTKTPRKSRRVATDCRLEFTGEDEVDGEAEVLDVSSTGCMAK
                     SDNRMSPGLHMKVALFLSDGHEWPVRVDEAVVRWARGGEFGVEFLAIRPAQLQRIQQF
                     VIKARPLS"
     gene            complement(961..1362)
                     /locus_tag="PK614_07765"
     CDS             complement(961..1362)
                     /locus_tag="PK614_07765"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB82145.1"
                     /translation="MHDRTIRSAMDQSNNLSSARIELRQHQRCVVPPACLLSFAPFAP
                     TISFNGDAEGEGVVINLSAGGCEISSDAGVTVGDAMSLIILLPGELCPTTIDLALVRW
                     GRDLHFGVEFVSMGTTEFNRIRQFLASTGPE"
     gene            complement(1482..2420)
                     /gene="lpxL"
                     /locus_tag="PK614_07770"
     CDS             complement(1482..2420)
                     /gene="lpxL"
                     /locus_tag="PK614_07770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HRB82146.1"
                     /translation="MSSTNTRKKRQRPHSSYHPLYWPTWVGLGLFRLLALFPYRWQLA
                     FGRQCGRLLHGLLTKRRDIVRVNLAVCFPRQEPVERNQVALHCFESMGMGVLEIAMAW
                     WTKDPRTYCECTIKGLEHIREALRQGRGVLLCGAHLHAAELAGRFMALEQPVAIVYRP
                     QNDVVAETVANRCRSRYYSELIQHRDMRGILRALARNQVVWYAPDIDAGSKRSVFAPF
                     FGVQAASLTATSRLAKVSGAAVVPCFYYRRADGSGYDIEVGPALEHFPSGEMVEDATR
                     INRLIEGAVRRVPEQYFWQHRRFKSRPPGEPPIYRR"
     gene            complement(2623..3003)
                     /locus_tag="PK614_07775"
     CDS             complement(2623..3003)
                     /locus_tag="PK614_07775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB82147.1"
                     /translation="MDGTTGQGQFSVDLRQHPRLRVSAPFVCSFSRLGLAKWLGRGGD
                     GIGGVFDVSMRGAKVMSEAGIQRGDRVSVTLALPNQVSPMTVEEATVRWEREQVYGLE
                     FVDLSPVAEMRLRKFITIATKPGA"
     gene            complement(3176..>3362)
                     /locus_tag="PK614_07780"
     CDS             complement(3176..>3362)
                     /locus_tag="PK614_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HRB82148.1"
                     /translation="AGAEEKGFGGHRMRKACAEDVKKLCPGVKAGEGRIAQCLKAHAA
                     ELSQTCSEMIRQRGKGR"
BASE COUNT          730 a         1077 c          941 g          614 t
ORIGIN      
        1 atgcaccagg acctcgatct gcgtctcggg aatatcgaag agccagcgcg cctcaatgac
       61 ctccagccct ttgttcatca gggtggccga gtcgatcgtg atcttggagc ccattttcca
      121 gttcgggtgc tgcagggcgc gctcaggatt gacgtcttga agttgctcgc gggagaagcc
      181 ccagagcggg cctcccgagg cggtcagaat ctgcctgacg agtacccgca gcgtctcccc
      241 acggccttgg cgtgtgggga gcacgaggtc cagcacggct tgcgtaaaga gtgtcgccag
      301 cgccgccagg tacgtatacc acgcgatggc cttgcgcccc acccactcaa tcatgcgttg
      361 tgagccgcct ttttcatgga gcaacaccgt cgggtcgagc caatattcat gtgggatccc
      421 gcgaaattgt aggggaagga tcggaaacaa agcaatgagt cgccctgccg gcgacgtctc
      481 gaaccggcgc agggagagga gatgccgtgt gggctcgtcg gcgtggacga aaacggcgga
      541 gcaacagcag gagggaggaa cgtgggacga ggcctatgac aacggcctgg ccttgataac
      601 gaactgctga atacgctgga gttgcgcagg gcgtatcgcc agaaactcca caccgaactc
      661 cccgcctcgc gcccatcgaa ccacggcttc atccactcgg accggccatt catgcccatc
      721 cgacagaaat aaagccacct tcatatgaag tcccggtgac atcctgttat ccgacttggc
      781 catacagccg gtcgaggaga catccaacac ctccgcctcc ccatccacct catcctcccc
      841 ggtaaattcc aatcggcaat cggttgcgac ccgacgagat tttcgcggcg ttttcgtctg
      901 ttccacccaa gctcctttct cagcacaccc cgcccagcct catcggcccc gataccggcc
      961 ctattcggga ccggtggagg cgagaaactg gcggatccga ttgaattcgg ttgtccccat
     1021 ggagacaaat tccacaccaa aatgtagatc ccgaccccat cgcaccaggg ccagatctat
     1081 ggtggtcggg cacagttccc ccggaaggag gatgatcaac gacatcgcat ctcccacagt
     1141 cacacccgcg tcgctgctga tttcgcaacc accggcggac aggttgatga cgacgccttc
     1201 accttccgca tcaccgttaa aactgatggt cggagcaaag ggtgcgaacg agagcaggca
     1261 tgccgggggc acaacacacc gctggtgttg ccggagttca atgcgcgcag aagagaggtt
     1321 gttggattga tccatggcgg agcggatggt gcgatcgtgc actacattca gccaggatca
     1381 gtatagcgca aggagccgga gacgccgaat acttctcatc attttgccag agccccgagt
     1441 cgaatacaca cgaccgcgcg acagcattga tggaaagcca cttagcgccg atagatcggc
     1501 ggctcaccgg gaggacggct cttgaagcgg cgatgctgcc agaagtattg ctccggaact
     1561 cgacggaccg caccttcgat cagccggttg atacgtgtgg catcctccac catttcaccc
     1621 gacggaaagt gctcgagcgc cggcccgact tcaatgtcat agccggatcc atcggctctt
     1681 cgataataaa agcagggtac caccgcggca ccgctgacct tcgccaatcg cgacgtggcc
     1741 gtcagggacg cggcctgtac accgaaaaac ggggcgaaga cgctgcgctt ggagcctgca
     1801 tcgatatccg gggcatacca caccacctgg ttcctggcta aggctcgcag gatcccccgc
     1861 atatcccggt gctgaatcaa ctcggaatag taccggctcc ggcatcgatt cgcgacggtt
     1921 tcggctacca catcgttctg tggccggtag acgatcgcca ccggctgctc cagcgccatg
     1981 aaccgaccgg ccagttccgc cgcatgcaaa tgggcgccgc agagcaacac gcctcgtccc
     2041 tgacggagcg cctcgcggat atgctccaat cccttgatgg tgcactcgca ataggtgcga
     2101 ggatccttcg tccaccaggc catcgcgatc tccagcaccc ccattcccat ggactcgaag
     2161 caatggagcg cgacctggtt acgttcaacc ggttcctgtc ggggaaaaca aacggccaag
     2221 ttcacccgga cgatatcgcg ccgcttggtc aataaaccat gcagcaagcg cccgcattgc
     2281 cgtccgaatg caagttgcca gcggtaggga aacagggcca gcaggcgaaa caatccgaga
     2341 ccgacccatg tcggccaata cagcggatgg taggaactat gcgggcgctg ccgcttcttt
     2401 cgtgtgttcg tactggacat ggccgaatgg ggaagccatt gtagggaaat ggagcggcga
     2461 gcgcaatctc gaagctatgc ggggaatcgc cacaacggga gctggcgagg gggacgcggt
     2521 tggaactgat gcaccaggac cgcaatgccg gcccgatgga ggctatgcgc ggttctgtgc
     2581 gccttcgcgc tactgtgccg ggcagatccc cgtaacgtgg ggttatgccc ccggtttcgt
     2641 cgcgatggta atgaactttc gcaggcgcat ctcggccacc ggcgacagat cgacaaactc
     2701 taatccatac acctgctctc tctcccaacg taccgtcgct tcctccaccg tcatcggcga
     2761 cacctggttg ggcaacgcaa gcgttaccga aactcggtcg ccacgctgaa taccggcctc
     2821 actcatcact ttggctcctc gcatcgacac atcgaacacg cccccgatgc cgtccccccc
     2881 gcgcccaagc catttggcca aaccgagccg ggaaaaggaa cagacgaacg gtgccgagac
     2941 gcgcaaacgc ggatgctgcc gcaaatcgac cgagaactgg ccctgtcccg ttgtgccatc
     3001 catacgtacc ctcccgtatg taatatggcc actgtatcgc acctgcgccg gttcgaaaaa
     3061 taaattgaga aaagcaaccg ggcgcaacgg aacgcaaaga gacggtgccg gacatggcct
     3121 gaccaatcgc cactttgccg acacctctcc tccactcaac ctaagccgga ggcatttacc
     3181 ggcccttgcc acgttgacgg atcatttcgg agcaggtttg tgagagttcc gcggcatggg
     3241 ccttcaagca ctgagcgatt cgtccttctc ccgctttgac ccccgggcag agttttttca
     3301 catcctcggc gcaggccttc cgcatgcgat ggcccccaaa gcccttctcc tcagcccctg
     3361 ct
//
LOCUS       DAORQN010000155         3360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_955_length_3360_cov_8.616339, whole genome shotgun sequence.
ACCESSION   DAORQN010000155 DAORQN010000000
VERSION     DAORQN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_3360_cov_8.616339"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..603
                     /locus_tag="PK614_07785"
     CDS             343..603
                     /locus_tag="PK614_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB82149.1"
                     /translation="MSKLLTMDEASEYLGISKLTLYGWVSARKLGFVKVGRLVKFKQE
                     HLDKWIEQHTVKARVQTQKFVIGGDRHIEERFGSGQLLGERE"
     gene            609..1730
                     /locus_tag="PK614_07790"
     CDS             609..1730
                     /locus_tag="PK614_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB82150.1"
                     /translation="MGLTKRPDSYYVEFRVIDSEDGKSLVLASGVPGARKKRWKVGCL
                     NKTIAREMETAIKTRILLGQERSERAKPILFKEWAQIYLALEEVKSLRSFVGRSHSVE
                     THLTPFFGGKLLSEIKPQDIEVFRAQRKKPNGTRASVQTINHDHIALKHCLNVAIRRG
                     LLQSNPASKIPLPNPHNERDRVLTDEEWGRLYRAAKAHLRPVLLTAYQLGQRFSEIVG
                     LTWDRVDVKRGFITLRSLDTKTKTARQVPMTPDVKMTLQRIGKTRSLATRHVFTYKGR
                     PLQRVSRSFKTALAEAGITDFRFHDLRHCASTNLRRAGVDTATAMKIVGHKSEKMWKR
                     YNAIEERDLTQAAQKVHRYLQQNTPGTLSEGSVEYSSHK"
     gene            1745..1821
                     /locus_tag="PK614_07795"
     tRNA            1745..1821
                     /locus_tag="PK614_07795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1779..1781,aa:Arg,seq:tct)
BASE COUNT          799 a          877 c          893 g          791 t
ORIGIN      
        1 gtaccgaggc actccgtagc gcgacttcgg gttcgattgc cgtgaactgt tcgaggaacc
       61 atgaggacca tcaacaggca tgtttgcctc cttattcatt ctatgtagcc ccacctttca
      121 caaaccaaag gtggggcgta gaagaaggag gcggcacggg gctctcgcag cgggcaggcc
      181 tggaggggcg gagaccggca cggaggggac ctggaaggcc tgccccgctg ctcctcggcc
      241 ccgggtcgta cccgctgggg tcggattatc cgacccattt cagcaaaagt atgggctaca
      301 taacttgttc aaatttgttc tgaaattcac tcggggagaa tcatgagcaa gcttttgaca
      361 atggatgagg catcggagta tctaggcata tccaaactga ctctctatgg ctgggtctcc
      421 gcgaggaagc tgggatttgt caaagtggga cgccttgtga agttcaaaca ggaacatttg
      481 gataagtgga ttgaacaaca cacggtaaag gcacgggttc aaacgcagaa gtttgtaatt
      541 ggtggcgata ggcacatcga ggaacgattt ggttctgggc agctacttgg ggagcgggag
      601 tagagatcgt gggactgacc aaacgacctg atagctatta tgtcgagttc cgcgtcattg
      661 acagtgagga cggaaagtcc ttggtgttgg cgagtggtgt gccaggagcg agaaaaaaac
      721 gctggaaggt cggatgtctt aacaagacca tcgccaggga gatggaaact gcgatcaaga
      781 cccgtattct cctcggacag gagagatccg aacgcgcgaa gccaatacta ttcaaagagt
      841 gggcacagat ctatcttgcg cttgaggaag tgaaatccct ccgctccttt gtcggtcgtt
      901 ctcatagcgt cgagacgcac ctgactccat tctttggcgg aaagctgctg tccgaaatta
      961 agccgcaaga tatcgaagtc ttcagggctc aacgaaagaa acctaatgga acacgcgcca
     1021 gtgttcaaac catcaatcat gaccacatag cgctcaagca ttgcctcaat gtcgcaatcc
     1081 gccgagggtt gctccaaagc aatcccgctt caaaaattcc tctgcctaat ccccacaatg
     1141 aacgggatcg cgtattgacc gatgaggagt ggggaaggct gtaccgtgcg gccaaggccc
     1201 atctcaggcc ggtactgctg accgcctatc aactcggaca gcgcttcagt gaaattgttg
     1261 gcctcacatg ggaccgtgtc gatgtgaaga ggggatttat caccctgcgg tctctcgaca
     1321 ccaaaaccaa gaccgctcga caggttccta tgactcccga tgtcaaaatg acattgcagc
     1381 gaatcggtaa aacacgtagt cttgctacca ggcacgtctt cacgtacaag ggacgaccgc
     1441 ttcagcgagt cagccgatcg ttcaagaccg cgctagcgga ggctggaata actgacttcc
     1501 gttttcatga tttgcgccac tgcgcttcta ccaatctccg aagggcagga gtggacacgg
     1561 caacggccat gaaaatcgtg ggccataagt cggagaagat gtggaagcga tacaatgcga
     1621 tcgaggaacg agacttgact caagctgctc aaaaggttca cagatacctt caacagaaca
     1681 cgccgggaac actgagtgaa ggttctgtcg aatactcatc tcacaagtga ttgaaaaata
     1741 atgcgcgccc gtagctcagt cggatagagc atcagccttc taagctgagg gtcgttggtt
     1801 cgattccaac cgggcgcacc actccatact ggcaggcagc caaatctttt aggtgcctga
     1861 acggaaggga caattcggga gttcgaaaac cctctttgcg gtggtaggcc attcgcttag
     1921 caaacaccag cctcaggacc attctcttgt cttccaatcg gtcggaactc caataatgac
     1981 aagggtttgc caagaacgtc attgcggttc gaaaacaatc ctcgaagggt tcgagcggac
     2041 gaccgcactt ctcgattttt tccctgagaa tgatcttttc gccctctagt ctggcgatct
     2101 cggtctcata tttctggata gctgtgtggt tgtcggtttc gacgagtcga ttgaggtatt
     2161 tttccgtccg ttgttccaga acgggaagct gggctttaat agcctgggcc tcatcattcg
     2221 ccaggcgctt ccggtcttcc cacagctctt tgaacatctc actggccata taaaacagat
     2281 tgggggtagg cctgagctgg ttcagcagga ttttaaagtc accctccagg gtttctttgc
     2341 gtatggattt tctatagtcg ggacagcctt tcgtggcgca caagtagtaa ggaaattttg
     2401 catatttgcc tgtggaccag cacgcagtca tgggcttgcc gcaccccgcg caggtcacaa
     2461 atccgcgcat cgggaaatca ctatgaaggt ctttccgggc aggagccttg gcgttgccct
     2521 gcaaccggtc ctgtatggct tgatacgtct cgaaactgat taagggctcg tgtttcgcag
     2581 gatgcagatg aaggttccac tcggggatgt tcatataacc ggcatagaga accttgccca
     2641 gcaggttggt caccttctga aaatggaccg tgccattcct gtttttagga tagtgcggcg
     2701 agccctccag aaacagccgg acttcatttt gagtttcaaa ccgtcctgag gcgtaccctt
     2761 ccagggcttc tctgactacc cccgccaccg gctcatcgac caccatcatt ttcccgccat
     2821 gtcctgggac atgagtaaat ttatagccga caggtggatt gaaggcgtag tatccattca
     2881 tcacccgcgc cttcatgcgg ttcttggtct gctcggcgtt cttttgccgc tgatgctggg
     2941 acacgctggc cagcaggttc tcaacaagca tgctgtcgga atcctcaccg aattcaatgg
     3001 aaggagaagc aagcttcgcc ccgaggccgc taatcaaagt tcgtagttga atgtgtgctt
     3061 ccaatccacg tgcgagccta ctgatatcat cgatcaatat gacatactgg tctcgctttt
     3121 gtgctttcag aaattttagc atctcctgca tgccgggacg gtcaatcatc cctcccgagg
     3181 ctccttcatc ccgaaacact ctgacgactt catagccctt attttgggca agctgacggc
     3241 atctggtctc ctgagagccg agcccatcgc ccttcttgac ctgtgcggcg ctggacacac
     3301 gtgcataaat gaccgcttta gagccctgtt gcggttccca ttcagtgcgc gatctgaaat
//
LOCUS       DAORQN010000156         3340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_961_length_3340_cov_5.700761, whole genome shotgun sequence.
ACCESSION   DAORQN010000156 DAORQN010000000
VERSION     DAORQN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_3340_cov_5.700761"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1556)
                     /gene="murF"
                     /locus_tag="PK614_07800"
     CDS             complement(93..1556)
                     /gene="murF"
                     /locus_tag="PK614_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRB82151.1"
                     /translation="MREHGNRGVMALFTVEEICEVLSVRPPAGVTPQDLKQRIRRVVT
                     DSRLVRKGDLFIAFQGERFDAHTFVPKVLAQGAVCAIVQEDYRLPPAPKRTGVPMLLG
                     VRDTLEAYQRLATHYRNRFPIPVIAITGSNGKTTTKEMVAQVVAQRWKTLKTEGNLNN
                     RIGVPQTLFQLAPRHQAAVIEMGVDQQGQTTRLCEIARPTIGVITNIGPDHLEFFGSM
                     EGSAQAKAELLDHLPQDGAVVLNADDEYFDYLAARAQCRVVAFGVSPKAAIRAANIRV
                     DEKGGTVFGLVVPGKSRQTEIRIRTQGQHNVSNALAAAAVGYALGLSRTAIAEGLAKF
                     RPAAMRSQISLSHGVQVVNDCYNANPASMKAAVQLLAELGRGKRSIAALGDMLELGTD
                     SKRMHREVGAFLAAQGIGHLLACGVLGRELAEGARQAGMASDSITELPDAAAAAVALA
                     RMVRQGDVVLVKASRGMRMEQVVEAVTGMRRVARKAC"
     gene            complement(1569..3077)
                     /locus_tag="PK614_07805"
     CDS             complement(1569..3077)
                     /locus_tag="PK614_07805"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRB82152.1"
                     /translation="MTLDELISPIHGRLGVLERNGDQRVTIADLTDDSRKVGPGSLFV
                     AVQGERVDGHQFLAPVVAAGAAAVVVQRSGDVGAISCVRVQDSRAALGILGSRFYGDP
                     SSSLRMIGVTGTNGKTTTTYVVKTMLEAARRRVGLIGTVAYLVGKESIPASHTTPGAL
                     ELQKLFARMREARLDTVVMEVSSHALALDRTAGSEFDVAVFTNLTQDHLDFHLDMERY
                     FQAKLKLFVELGRAGAVKQRKRAIINADDPWGARIRSACTVPVWTYGLHQQADIYAEG
                     VRLSPAGTSFTLRSPVGTCPIKSRLVGEHNVSNLLAAIGVVLHEGLTLEQVRSAVETV
                     TNVPGRFELVEAGQDFSVVVDYAHTEDALVRLLTAAQALRTGRIITVFGCGGDRDRTK
                     RPKMGRAAVQYSDVVILTSDNPRTEDPAAILREVELGVKEALADRGHVRYRMVADRRG
                     AIEAAIREAKPGDMVLIAGKGHEDYQIVGTTKHHFDDREVARDTIGALRSGA"
     gene            complement(3079..>3340)
                     /locus_tag="PK614_07810"
     CDS             complement(3079..>3340)
                     /locus_tag="PK614_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRB82153.1"
                     /translation="KTGTAQKVDPRTGAYSSTLLVGSFLGYVPAEDPRLAMIVVIDEP
                     RGEGWGGVVAAPVFRRVGEQVLNYLGVAVDEPVKLAMAGLES"
BASE COUNT          651 a         1172 c          893 g          624 t
ORIGIN      
        1 gaagggggta tagccagttg tataacatcc tacggcatct ttgcccgcca tggatcagcg
       61 ggccctaaag ttcctctata tgtgcctgcc gactagcagg cctttcgcgc gacccgtcgc
      121 attcccgtca cggcttccac gacctgctcc atgcgcatcc cgcgcgacgc cttgaccagc
      181 accacgtcgc cttgccggac catccgtgcc agggcaacgg ctgccgccgc cgcatcgggc
      241 aactccgtga tgctgtcgga cgccatcccg gcctgccggg ctccttcggc caactctcgt
      301 cccaatacac cacaggcgag cagatgtccg attccttgcg cggccagaaa cgccccgacc
      361 tcccggtgca tccgcttgct gtccgtgccc agctccagca tgtcgcccag cgccgcgatc
      421 gaccgttttc cccgaccgag ctccgccagc aattgaacgg ccgctttcat cgaggccgga
      481 ttggcgttgt agcaatcgtt gaccacctgc accccatgag aaaggctgat ctgcgatcgc
      541 atcgcggccg gccgaaattt ggccaaacct tcggcgatgg ccgttctcga taaccccaac
      601 gcatacccga ccgcagccgc ggcgagggca ttgctcacat tgtgttgccc ctgcgtccgg
      661 atgcgaattt cagtctgacg actctttccc ggcaccacca ggccgaacac cgttccaccc
      721 ttttcatcga cccgaatatt ggcggcgcgg attgctgcct tgggagacac cccgaacgcc
      781 acgacccggc actgcgcccg ggccgcgaga taatcgaaat attcatcatc ggcgttcaac
      841 accaccgccc cgtcctgcgg caaatggtcg agcaactcgg ccttggcctg cgcagaccct
      901 tccatactgc cgaaaaactc caaatggtcc gggccgatgt tggtaatcac gccaatcgtc
      961 ggtctcgcga tttcgcacag ccgggtggtc tgcccctgct gatccacccc catttcaatg
     1021 acggcggcct gatggcgcgg cgccaactga aacagagtct gagggactcc gatccgatta
     1081 ttgaggttgc cctcggtctt gagcgttttc cagcgctgcg caaccacctg ggcgaccatt
     1141 tccttcgtcg tcgtcttgcc gttgctcccc gtaatggcaa tgaccggaat gggaaaccgg
     1201 tttcgatagt gcgtggccag ccgttgatag gcctcgagcg tatctctcac cccgagcagc
     1261 atgggcacgc cggtacgttt cggcgccggc ggcaggcgat agtcctcctg cacgatggca
     1321 cagaccgccc cctgggccaa caccttcggc acaaaggtat gcgcgtcaaa ccgctcgccc
     1381 tggaatgcga tgaagagatc gcctttgcgc accaaccgcg aatcggtcac cacacgacga
     1441 atgcgttgtt tcaagtcctg aggcgtgacg ccggccggtg gtctgacact caagacctca
     1501 cagatttcct caaccgtaaa cagcgccatc acaccacgat tcccatgctc ccgcatgcgt
     1561 tccctcgctc aggctccgga tcggagcgcg ccgatcgtgt cgcgcgccac ttcccgatcg
     1621 tcgaagtgat gtttcgtggt tcccacgatc tgataatctt cgtgaccttt ccccgcgatc
     1681 agcaccatat cccccggctt agcctcacga atggccgcct cgatggcccc ccgacggtct
     1741 gcaaccatgc gatagcgaac atgaccccga tccgccagcg cctccttgac cccgagctcg
     1801 acctcgcgca ggatcgcagc aggatcttcg gtacgcgggt tatccgatgt cagaatcacc
     1861 acatcactat actgcacggc tgcacgtccc attttggggc gtttggtccg gtcacggtcc
     1921 ccgccacacc cgaagacagt gatgatgcgc cccgtgcgaa gcgcctgggc tgcggtcaac
     1981 aaccgaacca gcgcgtcttc ggtatgcgca tagtccacca ccacggaaaa gtcctgaccg
     2041 gcttcgacca gttcgaaccg ccccggcacg ttcgtgaccg tctcgacagc ggaccgtacc
     2101 tgctccagcg tcagcccttc atgcaacacc accccgatgg cagcgaggag gttcgacaca
     2161 ttatgctctc cgaccagacg gctcttgatc gggcaggtcc cgacgggact gcgcaacgta
     2221 aaactggtgc cggccggcga gagcctcact ccttcggcgt aaatatcggc ctgctggtgc
     2281 aggccgtagg tccaaaccgg aacggtgcag gccgatcgaa ttcgcgcgcc ccagggatca
     2341 tccgcattga tgatcgctcg cttccgctgc ttcaccgcgc ccgcccttcc taactcgacg
     2401 aagagtttca gctttgcctg gaaataacgc tccatatcga gatgaaagtc gaggtggtcc
     2461 tgcgtgagat tcgtaaagac agcgacgtcg aactcggagc cggcagtccg gtccaaggcc
     2521 aacgcatgag acgagacctc catcaccacc gtatccaacc tcgcctcgcg cattcgtgca
     2581 aataatttct gcaactccaa cgcgcccggc gtcgtatgcg atgctggaat cgattccttc
     2641 ccgacgagat aggcgaccgt cccgatgagt ccgacccggc ggcgagcggc ttccagcatg
     2701 gttttgacga catacgtcgt cgtggtcttt ccattggtgc ccgtgacacc gatcatccgc
     2761 aacgatgatg acggatctcc gtagaatcgg ctgccgagaa tcccgagtgc tgcgcgcgag
     2821 tcctgcacac gaacgcaaga aatcgcaccg acgtcgccgc ttcgctgcac cacgacggcc
     2881 gccgcccccg ccgcgaccac aggggcgaga aactgatggc catcgacacg ctctccctgc
     2941 acagccacga agagggaccc gggtcccacc ttccgcgaat cgtcagtcag gtcggcaatg
     3001 gtcacgcgct ggtctccgtt ccgttccagc actccgagac gcccatgaat cggactgatc
     3061 aattcatcga gtgtcatatc aggactcaag gccggccatg gccaacttga ccggctcatc
     3121 cactgccacg cccagatagt tgagtacctg ctcaccgacc cgacggaata ccggcgcggc
     3181 cacgacgcca ccccagcctt caccgcgcgg ctcgtcgatc acgacgatca tcgccagccg
     3241 aggatcttcc gccggcacat acccgagaaa cgacccgacc agcagcgtgg acgaataggc
     3301 gccggtgcgg ggatcaacct tttgagccgt accggtcttg
//
LOCUS       DAORQN010000157         3335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_963_length_3335_cov_5.003354, whole genome shotgun sequence.
ACCESSION   DAORQN010000157 DAORQN010000000
VERSION     DAORQN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_3335_cov_5.003354"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..573
                     /locus_tag="PK614_07815"
     CDS             34..573
                     /locus_tag="PK614_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82154.1"
                     /translation="MAPVAKVLLENFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARA
                     AGMSMIPTSTGAAKALHLVIPQLKGKLDGLAIRVPTPNVSLVDLTVETEKDCDVAAVN
                     AAFKKAAEGPMKNVLAYSDAPIVSIDLKDDPHSAIVDAPLTAVIDKRLVKVTAWYDNE
                     WGYSCRVRDLIKYIVAKGA"
     gene            669..1868
                     /locus_tag="PK614_07820"
     CDS             669..1868
                     /locus_tag="PK614_07820"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRB82155.1"
                     /translation="MNIRKRIIEDVQLRGKRVIIRADYNVPLDDSLQITDDTRIRSTL
                     PTINRAVDEGAKVILCSHLGRPKGRFDPKFSLAPVAKRLQRLLGKEVIFAPDCIGAAV
                     EGLVAKMQPGDVMLLENLRFHPEEEKNDDGFSKALASLADVYINDAFGAAHRAHASTV
                     GITKYIPEAAAGYLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKKVDK
                     VIIGGGMAFTFLKAMGLEIGQSLVENDMLDFAKGVQDHAFSSGVKFYLPVDCVVAASR
                     EPGAETKIVPVQEIPKGWYGLDIGPASVKLFSEAVQDAKTILWNGPMGMFEVDAFARG
                     TLAMAHSVANAYALTIVVGGETALAIHRAGESESISFISTGGGAALELLEGKTLPGLA
                     ALPNRAA"
     gene            1908..2687
                     /gene="tpiA"
                     /locus_tag="PK614_07825"
     CDS             1908..2687
                     /gene="tpiA"
                     /locus_tag="PK614_07825"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRB82156.1"
                     /translation="MRTRFIVGNWKMNKTASDAGAFVHRLSQELPHHAGIQIGLTPPF
                     TALHAVREALGPASAVLLGAQNLYWEDKGAFTGEVSGAMLKDIGCQFVLVGHSERRQY
                     FGDQDSWTNKKVLAALRHGLQPILCVGETLQDRDSEQTDLVIQRQLRAGLAGIEAHTL
                     PTLTIAYEPVWAIGTGRAASPEQAVPVHRLIRRTIGELSGTDTGNHVRILYGGSVTPH
                     NIADFLASEEIDGALVGGACLDPVSFATLVKVAIGSKPTTV"
     gene            2697..3050
                     /gene="secG"
                     /locus_tag="PK614_07830"
     CDS             2697..3050
                     /gene="secG"
                     /locus_tag="PK614_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRB82157.1"
                     /translation="MYTLLIIIHVIVCLLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFTVITAFVFMFTSLSLAIIAKDRNFASTVIDLNKKPAATTPSSTPSETT
                     TPPAAESHSSGEGSH"
BASE COUNT          761 a         1010 c          884 g          680 t
ORIGIN      
        1 atcgtatcca acgcatcctg aaccaccaac tgcctggccc cggttgccaa agtcttgctg
       61 gaaaacttcg gcatcaaaca cggcgtcatg acgacgattc attcctacac gaacgaccag
      121 caactgctcg atcttcccca caaggacctc cgccgtgcgc gggcggccgg catgtcgatg
      181 attccgacca gcaccggtgc ggccaaggcg cttcacctgg tgatcccgca actgaagggg
      241 aaattggatg gactggccat ccgggttccc acccccaatg tttccctggt cgacctcacg
      301 gtcgaaaccg aaaaagattg cgatgtcgca gcagtgaacg ccgctttcaa gaaagccgcc
      361 gaaggtccga tgaaaaatgt cctggcttat tcggatgcgc cgatcgtctc gatcgatctc
      421 aaggacgatc cacactctgc gattgtcgat gctccgttga ccgcagtgat cgacaaacga
      481 ctcgtcaagg tcaccgcctg gtacgacaac gagtggggct attcctgccg cgtgcgggac
      541 ctgatcaaat acatcgtcgc caagggggcc tgagccgact cttgcacaac gggcttttct
      601 gacgtgcagc ccaaatcggc gtgaatatcg ctaggctgca cacaccggac gaggagtgaa
      661 ggtctgtcat gaatattcgc aaacgaatca tcgaggatgt ccagcttcgt gggaaacgcg
      721 tgatcattcg cgccgactac aacgttccgc tcgacgattc attgcagatc accgacgaca
      781 ccagaattcg atccacgctt cccaccatca atcgtgccgt tgatgaggga gcaaaggtca
      841 tcctctgctc ccacctcggc cggccaaagg gcagattcga tccgaaattc agtctggccc
      901 ccgtcgcaaa acgtctccag cgcctgctcg gcaaagaagt gatttttgcc ccggattgca
      961 tcggggcggc tgtcgaaggt ctcgtcgcca agatgcagcc cggcgacgtc atgctactgg
     1021 aaaatctccg tttccatccg gaagaagaga agaatgacga cggattctcc aaggccctgg
     1081 catccctcgc cgatgtgtac atcaatgacg ccttcggtgc cgctcaccgg gcccatgcct
     1141 ccacagtcgg tatcacaaaa tatattcctg aagccgccgc cggttatctc ctcaagaaag
     1201 aaattgaata tctcgaaggc gccgtagaaa atccggtccg accgttcgtc gcaattcttg
     1261 gcggagccaa agtgtccggc aaaatcggcg tgatcgagaa cctcggaaaa aaagtcgaca
     1321 aagtcatcat cggcggcggc atggctttta ccttcctcaa agccatgggg ctggagatcg
     1381 gccaatcgct ggttgaaaac gacatgctcg acttcgccaa gggtgtgcaa gaccatgcct
     1441 tttcaagcgg cgtcaaattc tatctcccgg tggactgtgt tgtcgcggcc agccgtgaac
     1501 cgggggctga aacaaagatc gttccggttc aggaaattcc caaaggttgg tacggactgg
     1561 atatcggccc cgcctcggtc aaactattct cagaagcagt tcaggatgcc aagaccattc
     1621 tctggaacgg tcccatgggc atgttcgaag tcgatgcctt tgccagaggg accctcgcga
     1681 tggcccattc cgtcgccaat gcctacgcct tgaccatcgt cgtgggaggc gaaaccgccc
     1741 tcgcaatcca tcgggccggt gaatccgaaa gtatctcgtt catttcaacc ggcggtggag
     1801 cggcgctgga attactcgag gggaaaaccc ttccgggact cgcagccctt cccaaccgtg
     1861 cagcatgacg acaacgtcta ccgtttcctc cccgcaatag aggctccgtg agaactcgct
     1921 tcatcgtcgg caactggaaa atgaataaga ccgcctcgga cgccggggca ttcgttcatc
     1981 gtctatcgca ggaattgccc caccatgccg gaatccagat cggcctgaca ccgccattta
     2041 cggcgctgca cgccgtacgc gaggcgctgg gcccggcctc ggccgttctc ctcggcgcgc
     2101 aaaatctcta ttgggaagat aaaggagcat tcaccggaga agtgtccggg gcgatgctga
     2161 aggacatcgg ttgccagttt gtcctcgtcg gccattcgga gcggcgacag tatttcggcg
     2221 atcaggacag ctggacgaat aaaaaagttc tagccgccct tcggcatggc cttcaaccga
     2281 ttctgtgcgt gggagaaaca ctccaggatc gtgattcgga acagaccgac ctcgtcattc
     2341 aacgacagct tcgcgccgga cttgccggga tcgaggcaca cacacttcca accctgacga
     2401 tcgcctatga gccagtgtgg gccattggaa ctgggcgggc tgcgtcaccg gagcaggcgg
     2461 tcccggtcca tcgcctcatc cgccgaacca tcggcgaact gagcggaacg gacacaggaa
     2521 accacgtccg cattctatac ggagggagcg tcacaccgca caatatcgcc gactttctag
     2581 catccgaaga aatcgacggc gcacttgtcg gcggggcttg tttagacccg gtctcttttg
     2641 ctacactcgt caaagtcgca atcgggtcca aacccaccac ggtctaaaga ctcaacatgt
     2701 atacactgct catcatcatc catgttatcg tctgcctgct catgattgga gcaattctgc
     2761 tgcaatccgg taagggcgct gaaatcggtg cggcatttgg tggctccagc caaaccgtat
     2821 tcggcagccg agggccggct aacttcctga gcaagttcac cgtcatcacc gcatttgtct
     2881 tcatgttcac ttcattgtcg ttggccatca tcgcgaagga ccggaatttc gcatctacgg
     2941 ttatcgactt gaacaagaaa cctgctgcaa caactccaag ttcaacgccg tcagaaacca
     3001 cgacgccccc tgccgcagaa tctcattcat ccggcgaggg ttcgcactag cctcctcgcc
     3061 tcacagctcc tactgcatca caggtatcag ccgagcacat gacgaaagcc cgttcgccac
     3121 agcgacgggc tgtagcgaat tcgcatgcta tcgagatgtt cgcgtgggga agatactgtg
     3181 atcgatgaga ctgctagacg ggagtcagcc aatgccggtg agcggaatca gtcccgccca
     3241 ccacatcaaa aaatgctttt tgtaacgcct gcgtgatcgg ccctggcttt ccgctgccga
     3301 tgcgccgctg atcgatttcc gtcacagggg tgagt
//
LOCUS       DAORQN010000158         3329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_965_length_3329_cov_19.607208, whole genome shotgun sequence.
ACCESSION   DAORQN010000158 DAORQN010000000
VERSION     DAORQN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_3329_cov_19.607208"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1966)
                     /locus_tag="PK614_07835"
     CDS             complement(215..1966)
                     /locus_tag="PK614_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82158.1"
                     /translation="MKILFFAPHAAIWVHAFPEALIAETLAQSGHAIVYVSCGEVLQQ
                     VCIPMNAFGLDHLSPVDARRKICRLCGEQKRLLRQEFGFPGCDLADLLEPADRIQAQE
                     MVDALSFEECMALTVDHVPVGTLALYETILNRKKTGIEFDASEQTEYRAWLRGAVTTV
                     IAGTRLFEREQPDCMAVYNSFYSVNHVMCRLASVRGIPHYFLHAGGNLSRRLQTLMVG
                     REEAYRYLRGLVDRWPQFRDLPCAPDTLRDITNHFLVLFGGRSVFGYSPGASRESFDV
                     RQRFGVRMDQKIMVATMSSKDERVAAKAIGILDDQPQMLFPQQVDWVKRLIKFVDGRP
                     DLFLLIRVHPRDFPNKREAVKSEQAKDLETIFGQLPHNVRVNWPSDGVSLYDLANETS
                     VFLNAWSSVGKEMSLLGLPVVIYAPALIYYPSELNYVGSTEQEFVEKIDQALRDGWSL
                     DRCRATYRWLAVEYGHGLIDIADSYPAKDDGARSIVHRIAKRLERFVAPLYQQQQDCK
                     RRAERLRASQMVDSIFRSGAATPVDPKVWMPAPASTQDEEDRVLRSELVRIGKTLFGS
                     GTIHRRGTLADRLAALG"
     gene            complement(1963..2718)
                     /locus_tag="PK614_07840"
     CDS             complement(1963..2718)
                     /locus_tag="PK614_07840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB82159.1"
                     /translation="MCVDLGSHTYRRAYLRCALCRHWYSENPLDLSRLYESAYVSATY
                     GDRFRETFLRILALPPERSDNTARVARVLRFAHACVTPGKTPTLLDIGSGLGVFPYRM
                     KEAGWDCTVLDPDARAVEHATTVVCVRAIKGDFLTIDPDQVGRFDLMTFNKVLEHVID
                     PIGMLQRATGFLRPNGRVYIELPDGEVASQHGSQREEFFIEHHHVFSAGSLSMLAERA
                     GFLTLSVERLHEPSGKFTLCAFLAPKSIVLAAP"
     gene            complement(2799..3185)
                     /locus_tag="PK614_07845"
     CDS             complement(2799..3185)
                     /locus_tag="PK614_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82160.1"
                     /translation="MKFSPKNPPRRFEVGHDVRFELQDCGTAYMQPNEQITFVTESGS
                     QYDVVRKDWGFYATPSLNARLPQCGFRGALVVNHDSRRYYVLLVENGKTQQFAAYCVR
                     ERLRVVAWLDDTDTVESIFGGSVDRP"
BASE COUNT          796 a          980 c          843 g          710 t
ORIGIN      
        1 atgtagtccc tgcgcccgaa tcgaaatagt cagaacgtct ttcttgtgcc gacttctggc
       61 cctcgctcga caagggagaa ttttcaatct tcatacagtc cccatgatca tggatgtcct
      121 ttgttcagac ggcccgctca aagcggccag cgattgcttt gacgcccctc gcaatacatt
      181 ggcccgtcaa gccaagagcg agttgcgcgt ctgtttagcc gagagccgct aggcggtcgg
      241 ccaacgtgcc cctcctatga atcgttccac tgccgaacaa cgtctttcca atacggacca
      301 gttcggaacg gagcacgcga tcttcttcat cctgagtgct cgcaggtgca ggcatccaga
      361 ctttcggatc caccggtgtt gctgcgccgg atcgaaagat cgaatccacc atctgagaag
      421 ctcgaagccg ctcagccctt ctcttgcaat cttgttgttg ctgatacagt ggcgcgacga
      481 agcgctccag gcgtttcgcg atgcggtgaa cgatcgagcg cgcgccatca tcctttgccg
      541 gataactgtc ggcaatgtcg atgagaccat ggccgtattc gacggctaac catcgatagg
      601 tggctcggca cctgtcaagg ctccacccat ctcgaagcgc ttgatcgatt ttctcaacaa
      661 actcctgttc cgtcgaaccg acataattca gctcagaagg ataatatatc agggcaggtg
      721 cgtagatcac gaccggtagc cccaacaaac tcatttcctt gccgacgctc gaccacgcgt
      781 ttaaaaatac ggacgtttca ttcgcgaggt catacagaga caccccgtca ctcggccaat
      841 tgacgcgcac gttgtgtgga agttggccaa aaatcgtttc cagatccttg gcttgttctg
      901 actttacggc ttctcgctta ttcggaaaat cacgcggatg cactcgaata agcagaaaga
      961 gatccgggcg cccatcgaca aatttgatga gccgtttgac ccaatccacc tgctgaggaa
     1021 ataacatctg gggttgatcg tcgagaatac cgatagcctt ggcagccacg cgttcgtctt
     1081 tgctgctcat ggtcgccacc ataattttct gatccattcg gacaccaaat cgttgacgaa
     1141 catcgaatga ttcccgtgat gctccaggag agtagccaaa cacgcttcgg ccgccgaaca
     1201 acaccagaaa gtggttggta atgtctcgca gagtatcagg cgcgcatgga agatcgcgaa
     1261 actgcggcca acggtcgacg agaccccgta ggtatcgata cgcttcttct ctgcccacca
     1321 tcagcgtctg cagccgacgc gagaggttgc cgccggcatg caaaaagtaa tgaggaattc
     1381 ctcggacgct tgctaaacgg cacatcacgt ggtttacgga atagaatgaa ttatagacgg
     1441 ccatgcagtc aggctgctcc cgctcaaaca ggcgtgtgcc ggcaatcacc gtggtaaccg
     1501 ctccgcgcaa ccacgctcga tactctgtct gttcgcttgc atcgaattca atgccggttt
     1561 tcttccgatt gaggatcgtt tcatacaatg cgagcgttcc gacgggaaca tggtctaccg
     1621 tcaaggccat gcactcttcg aacgacaagg cgtccaccat ttcttgtgcc tggatccgat
     1681 cggcgggctc cagaagatcc gccaaatcac atcctgggaa accaaactct tgcctcaaca
     1741 acctcttctg ctctccgcac aaccggcaga ttttgcggcg cgcgtcaacc gggctgaggt
     1801 gatcgagccc aaaggcattc ataggaatac agacctgctg caggacctct ccgcagctca
     1861 catagacgat cgcatgcccg gattgagcta atgtttcggc aatgagcgcc tccggaaacg
     1921 catgcaccca gatcgcggca tgaggagcga aaaatagaat cttcacggcg cagcgagtac
     1981 gatggatttc ggcgcaagga atgcgcacaa cgtgaatttc ccactgggtt catgaagtcg
     2041 ctcgacagac aaggtcagga accccgcacg ctccgccaac atgctgagcg agccggcgct
     2101 gaaaacatgg tgatgttcga tgaaaaactc ttcgcgctgc gacccatgct gtgacgcaac
     2161 ctcaccgtcg gggagctcga tatacacccg cccattcggc ctcaaaaacc ctgtcgcccg
     2221 ctgcaacata ccaatcgggt cgatcacatg ctccaggact ttattaaacg tcatcagatc
     2281 gaagcgccca acctggtcgg gatcgatcgt gaggaagtct cccttgatcg ctctcacgca
     2341 gaccacagtc gtcgcatgct ccactgctcg ggcatcagga tcgagcacgg tacagtccca
     2401 cccggcctcc ttcatacgat acggaaacac tccgagtccc gaaccgatgt cgagaagcgt
     2461 cggcgtcttg ccgggtgtca cgcaggcatg agcgaatcga agaaccctcg ctacgcgtgc
     2521 ggtgttatca gacctttccg gaggaagagc gagaattcgc agaaaagtct cccggaacct
     2581 gtctccgtaa gtcgcactga cgtaggcact ctcatacaga cgagaaagat cgagcggatt
     2641 ctctgaatac cagtgccgac agagagcgca tcgcaaatag gcgcgccgat acgtatgcga
     2701 gcctaggtca acgcacattt cgccgggtgg gggtgcatca tagcgaaaca cggtctcgca
     2761 gtaattcaaa ttgcaggggc attgcagggt gggacgcatc atggacgatc cacggagccg
     2821 ccgaatatgg attccaccgt gtcggtgtca tccaaccacg ccacaactcg cagccgttcc
     2881 cgcacgcagt atgcagcaaa ctgctgcgtt ttcccattct ctaccagcaa cacgtaatag
     2941 cgtctcgaat cgtgattgac caccaaggct ccgcggaaac cgcactgcgg gagtcgagcg
     3001 ttcagcgacg gtgtcgcgta gaagccccag tccttgcgaa ccacatcata ttgcgaccct
     3061 gattccgtca cgaacgtgat ctgttcgttc ggctgcatgt aggctgtacc acagtcctga
     3121 agctcgaatc gaacatcatg cccgacctcg aaacggcggg gcggattctt gggagagaat
     3181 ttcatgccca gtgccaatcc tcaatcaaaa aatccggtat atgccaggct ccggatttct
     3241 ctctcttcca gatagaatga ttgcgaaatg cttcacatgt tttccagaat tgtgcctgct
     3301 caatcccgac gaaacgacaa aacttttgg
//
LOCUS       DAORQN010000159         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_966_length_3324_cov_15.944937, whole genome shotgun sequence.
ACCESSION   DAORQN010000159 DAORQN010000000
VERSION     DAORQN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_3324_cov_15.944937"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..820
                     /locus_tag="PK614_07850"
     CDS             26..820
                     /locus_tag="PK614_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82161.1"
                     /translation="MSGSQSPWRYRNLNGDAGKIWHHPFNGLPIPVVDQIITENTPHL
                     GTGGLVSYFNHFHEWTGPVAEGTAGGWTLTGTTGTATIVHSDTKVGAIVLTTDNTGSA
                     TFALQRGNGTTGMNLAYTVGKRMWFFIRFKLLTVASMELFMGLGTADTSPSTTGTFPS
                     DGIFFEKAAAATVLDFHARKDGTSTEKTSVSGTLVDDTYTIIGFNIDELGNIMAYQDG
                     AAIAASAIAAGTANIPAATDVMQLNLAFIGQAMTMTVDWVLCAQEL"
     gene            820..1131
                     /locus_tag="PK614_07855"
     CDS             820..1131
                     /locus_tag="PK614_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRB82162.1"
                     /translation="MDYEIFAMQDKVAVKLTPPEERIGNILLPDTAKGDNHIGTIVSV
                     GIATTGVLLQVGDLVVVPRYAGSPVKIDGEELLFMKSTDVLAKLLPIPRGVEAAHAGH
                     D"
     gene            1115..2074
                     /locus_tag="PK614_07860"
     CDS             1115..2074
                     /locus_tag="PK614_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82163.1"
                     /translation="MPVTIETVARQLIASVDSDAGYLLASQWIVKRYEQLAIKAKLRH
                     LRQVGLVSTPATITTGTVTLTRGSKVVSCDATALAAWTTSLVGYHLRARVNWYEIVEH
                     NLTTGALLLAGAYEEDSVVDGTYIIVQRWVPLATDVGYLGDTFINQRRRHPLSLRDLG
                     ELDRSAPSRSAIAGSATVVVEAPQLPDGRKRVEFYPYSTTSESYAYVYWRSVGQLTYS
                     DYLPPSVPAYILIEGALIDLFRYKMAQAINQPGGGESAAFWRNEMRTQETKWKDHLVE
                     AIGADRGEDDTSFILKHLGEELGPTDITTARDHILAQWNWPSS"
     gene            2102..2761
                     /locus_tag="PK614_07865"
     CDS             2102..2761
                     /locus_tag="PK614_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82164.1"
                     /translation="MPDVAKTLTDQVLRRVRDVHGLAHSRALVRTLLSHSQRLLNSLL
                     GIVTTSTSFTTYAHQQFYQISGLLTGTDAITKVMAVKDGPRDLTHLANIRQLNHLDMR
                     WVRAIGPRFDAWTQLGRDMLIIYPAKTLNSTVTIVGAKLTTELTGEDTALELPNEYHD
                     HIISLTEIMLLAKQRDLTQAVRQLQRLSHALKTDTIPLKLHVGDNPAMVNAGTVAPQK
                     Q"
     gene            2776..3153
                     /locus_tag="PK614_07870"
     CDS             2776..3153
                     /locus_tag="PK614_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82165.1"
                     /translation="MKAIMLILTAALFAACAPMVNVDKIGNEYAQHAYSRNFWWPNYD
                     RTTFCWKLDANGYCPKEDTRIETHTAIAQEAAGQYAAKSAVASMPMALGLGLGLAHSG
                     SRMTQSVGGLTINETFSTKYIGR"
BASE COUNT          736 a         1092 c          857 g          639 t
ORIGIN      
        1 tctttgattt gagaaggaga gtcctatgtc tggttcacaa tccccatggc ggtatcgcaa
       61 cctcaatggc gacgcgggga aaatttggca tcacccgttt aatgggctgc cgatcccagt
      121 ggtcgatcaa atcatcaccg agaacacccc ccaccttggg acgggcggcc tcgtctcgta
      181 cttcaaccac ttccacgagt ggacgggtcc cgtcgcagaa gggaccgcag gcgggtggac
      241 gctcaccggc actaccggca ccgccaccat cgtccacagc gataccaaag tcggcgcgat
      301 cgtcttgacc accgacaaca ccggatcggc tacgtttgcc cttcaacgcg gcaacggcac
      361 aaccggcatg aacctcgcct acaccgtcgg caagcggatg tggtttttta ttcgcttcaa
      421 gctcctcacc gtcgccagca tggaactgtt catgggattg gggacggccg acacctcccc
      481 gtccacgact ggcacgttcc ccagcgatgg gatctttttc gagaaagccg ccgctgccac
      541 ggtgcttgat ttccacgccc gcaaggacgg cacgagcacc gagaaaacca gtgtcagcgg
      601 cacactcgtt gacgacacct acacgatcat cgggttcaat atcgacgaac tcggcaatat
      661 catggcctat caagacggcg cagcgattgc ggccagcgcg attgcggccg gcaccgccaa
      721 catccccgcc gccaccgatg tcatgcaact gaacctcgca tttatcggtc aggctatgac
      781 gatgacggtg gattgggtgc tctgcgcaca ggagttgtaa tggactacga aattttcgcc
      841 atgcaagata aggtggccgt gaagctcact ccccccgagg agcggatcgg caacattctc
      901 ctccccgaca cagcgaaagg cgacaatcac atcggcacga ttgtatcggt ggggatcgcc
      961 accactgggg tcctgcttca agtcggtgat cttgtggtcg tccctcgcta cgctggctcc
     1021 cccgtcaaaa tcgacggcga ggagctactg tttatgaagt cgaccgacgt gttggccaag
     1081 ctgttgccga tcccgcgtgg cgtcgaggcg gcccatgccg gtcacgattg aaaccgtcgc
     1141 acgccaactg atcgccagtg tcgacagcga tgccgggtat ctgttggcgt cgcaatggat
     1201 tgtcaaacgc tacgagcaac tcgcgatcaa agccaagctt cgccacttac gacaagtcgg
     1261 gctcgtctct acccctgcca cgatcacgac cggtaccgtc accctcactc gcggctccaa
     1321 agtcgtgtcg tgtgatgcga cggcactcgc agcctggacc acctccctcg tcggctacca
     1381 cctccgcgcc cgcgtcaact ggtatgagat cgttgagcac aacctgacga ctggtgcctt
     1441 gctgctcgct ggcgcctacg aagaagattc cgtagtggat ggtacgtaca ttatcgtcca
     1501 acgctgggtc cccctcgcca ccgacgtagg ctacctcggc gacacgttca tcaaccagcg
     1561 tcgacgccat ccgctcagcc tgcgtgacct cggcgagctg gatcggtctg ccccatcgag
     1621 gtccgccatc gctggatccg ccacagtggt tgtggaggct ccccaactgc cagatggtag
     1681 aaagcgagtg gaattctacc cctactccac caccagcgag tcctacgcct atgtctactg
     1741 gcgatcggtg gggcagctca cctacagcga ctatttaccg ccgagtgtcc cagcctatat
     1801 cctgatcgag ggcgccctca tcgacctgtt ccgctacaaa atggcccaag ccatcaatca
     1861 acctggtggt ggtgagtccg ccgccttctg gcgcaacgaa atgcgcaccc aggagaccaa
     1921 atggaaagat cacttggtgg aagcgatcgg cgcggatcgg ggcgaggacg acaccagctt
     1981 cattctcaaa catctcggcg aggaacttgg tcccaccgat atcacgaccg cccgcgacca
     2041 tatcctcgcc cagtggaatt ggccgagcag ctaaccatcc cgcaactcga aaggtggtct
     2101 cgtgcccgat gtcgccaaaa ccctcactga ccaagtgctc cgccgcgtgc gtgatgtgca
     2161 tggcctagcg cactcacgag cactcgtccg cacgctgctc tcccactccc agcgtctcct
     2221 caacagcttg cttgggatcg tcaccacctc taccagtttc accacctacg cccatcagca
     2281 gttctaccaa atctccggcc tgctgactgg gactgacgca atcacaaaag tcatggcggt
     2341 gaaggacggc ccccgcgacc tcacgcacct tgccaacatc cgccagctca atcacctcga
     2401 tatgcgatgg gtgcgtgcga tcgggccgcg gtttgatgcg tggactcaac ttggtcgcga
     2461 tatgttgatc atctaccccg ccaagaccct caactccacc gtgacaatcg taggcgccaa
     2521 gcttaccacc gaactgaccg gcgaggacac cgcactcgaa ctccccaacg agtaccacga
     2581 tcacatcatc tcgttgactg agattatgtt attagccaaa caacgcgacc tgacccaagc
     2641 cgtccgtcaa ctccagcgtc tctcacacgc actcaaaacc gacacgatcc cactgaaact
     2701 ccatgtgggt gacaaccccg cgatggtcaa tgccggcacc gtcgcaccac agaagcaatg
     2761 aaaggatacg cacctatgaa agcaatcatg cttatactca ctgctgcttt gtttgctgct
     2821 tgtgctccaa tggtcaacgt cgacaaaatc ggcaacgagt acgcccagca cgcctactcc
     2881 cgcaacttct ggtggccgaa ctacgatcgc accaccttct gctggaagct cgacgccaac
     2941 gggtattgtc ctaaagagga cacccgcatc gaaacccata ccgcgattgc ccaagaagcc
     3001 gcgggtcaat acgccgcaaa atccgccgtg gcgagtatgc cgatggcgct tggattgggt
     3061 ttgggactgg cgcactccgg ctctcgcatg acgcaatcgg tgggtgggtt gactatcaac
     3121 gaaaccttct ccaccaagta tattgggaga taaccgaaag ggggtgcgat gaaccccatt
     3181 gaacaactca tcaatttctt gaacgtgctg ctcgcctgca ccgcgatggc caccacagaa
     3241 cagaccatta gcggccgcga tttcctcgtg cagcactggc aatgtaaaac taccaacgcc
     3301 gtcacgcaca tcgacgggtg gcgg
//
LOCUS       DAORQN010000160         3291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_969_length_3291_cov_8.047590, whole genome shotgun sequence.
ACCESSION   DAORQN010000160 DAORQN010000000
VERSION     DAORQN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_3291_cov_8.047590"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="PK614_07875"
     CDS             <1..1490
                     /locus_tag="PK614_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82166.1"
                     /translation="TNARWSDNEMHDAMQVYWSRFAGKAQWSLWVFFARLHEQGTSLG
                     GIMFDDIGPNHRQGTAIFNESFISTAPTGDAKPAAWVERMRFWTACHEMGHAFNLAHS
                     WQKQHPPDWGTPWIPLANEPEARSFMNYPYNVSGGQTAFFSDFAYRFSDNELVFMRHA
                     PERFVQMGNADWFDHHGFEQASASPEPALKLNLRVDRAQATYQFLEPVVLELKLTNIG
                     SRPLVLEKELLSMTDHMTVIVKKRDKPARQYLPFARYCHDMQAQVVMPGEFVTDSLFI
                     SVGRNGWDIAEPGYYTIQIALHMETEDIVSESLTIRVAPPRGYDEEFIAQDFFSDDVG
                     RILNFDGSAILRRGNDTLREVSDRFGDRAVANHARVALAAPLARDYKVLDMGDRMGEM
                     ASAKEAGGRLRRAAPRIEEARQLYTSALLEKKDQAIATLGRTDYEYYLGRFRESLMEH
                     GAVLKKEPRDAKRDAKREKNGDIEHTMRVIKETLSSLKDQEPVSR"
     assembly_gap    1510..1542
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1561..2007)
                     /locus_tag="PK614_07880"
     CDS             complement(1561..2007)
                     /locus_tag="PK614_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB82167.1"
                     /translation="MTHTIRQATIDDVEQLVPLFDAYRQFYGRTSDVAAARALARCAN
                     KESTLFIAHEGDTAIGFAQLYPSFSSVSLARIFILNDLFVQEQARRTRVASSLLSAAA
                     TFAVSLGAVRLSLSTALTNDAAHALYRSAGWNRDQQFSVYHLALSV"
     gene            complement(2102..2383)
                     /locus_tag="PK614_07885"
     CDS             complement(2102..2383)
                     /locus_tag="PK614_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRB82168.1"
                     /translation="MPHVLIIHEVEAYPAWKAVFDGAADLRKRAGEISYQLLRYDQDA
                     NRVVHFSTWSSLENARRFFESPELVKLRRAAGVKAPDFLYLEEIERGVL"
     gene            complement(2650..2979)
                     /locus_tag="PK614_07890"
     CDS             complement(2650..2979)
                     /locus_tag="PK614_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HRB82169.1"
                     /translation="MARSNQSQREQTYRERALKLFPWICAHCGRDFDGKKLSQLTVHH
                     KDHNHDNNPPDGSNWELLCLYCHDNEHQRYQAADASDETPPRRKQDQPSTFTPFAHLA
                     DMLKRRT"
     gene            complement(3097..>3291)
                     /locus_tag="PK614_07895"
     CDS             complement(3097..>3291)
                     /locus_tag="PK614_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HRB82170.1"
                     /translation="VLGNVCPNCGGGFVPRPIRPSKNWKGDNFLGKDPASTTIKHRPV
                     DLPAHELFSAAIKTIPPEKR"
BASE COUNT          684 a          866 c         1036 g          672 t
ORIGIN      
        1 gtacaaatgc gcgttggagc gacaacgaaa tgcatgatgc gatgcaagtg tactggtcgc
       61 gatttgccgg caaggcgcag tggtcgctct gggtgttctt tgcccggttg cacgagcagg
      121 gcacgagcct gggcggcatc atgttcgacg atatcgggcc gaaccatcgc caagggacgg
      181 cgattttcaa cgagtcgttc atctccacgg cgccgaccgg cgatgccaaa ccggcggcgt
      241 gggtcgagcg gatgcgattc tggacggcat gccacgaaat gggccacgcg ttcaacctgg
      301 cgcactcgtg gcagaagcaa cacccgccgg actgggggac cccgtggatt ccgttagcca
      361 acgagccgga agcccgcagc ttcatgaact acccgtacaa cgtgtccggc gggcagacgg
      421 cgtttttttc cgacttcgcc tatcggttca gcgacaacga gttggtattc atgcgccacg
      481 cgccggagcg gtttgtgcag atggggaatg ccgattggtt cgatcatcat ggattcgagc
      541 aggcgagcgc gtcaccggag cccgcgttga agctcaatct tcgcgtcgat cgagcccagg
      601 ccacctatca attccttgaa ccggttgtgc ttgagctcaa gctgaccaac atcggttcgc
      661 gtccactggt gctggaaaag gaattgctgt ccatgacgga tcacatgacc gtcatcgtca
      721 agaagcggga caagccggcc cggcagtatc tgccctttgc gcgctattgc cacgacatgc
      781 aggctcaggt ggtgatgccc ggggaatttg tgaccgattc gctctttatc tctgtcgggc
      841 ggaacggctg ggatatcgcg gagccgggat attacacgat ccagatcgcg ctccatatgg
      901 agacggagga tattgtctct gagtccctga ccatccgtgt cgctcctcca cggggctacg
      961 acgaagagtt tattgcgcag gacttcttct ctgatgacgt cggacgcatc ctcaatttcg
     1021 acggcagcgc catactccgt cgagggaacg atacgctcag ggaggtgagt gaccggttcg
     1081 gcgatcgtgc ggtggccaat catgcgcgtg tggccctggc tgcgccgttg gccagggact
     1141 acaaggtgct cgacatgggc gatcggatgg gggagatggc gtctgcaaag gaggccggag
     1201 gccgtctccg tcgcgccgcc ccccgtattg aggaggcacg gcagctctat acgagcgctc
     1261 tgcttgagaa gaaagatcag gcgatcgcga ccttgggacg gaccgattat gaatattatc
     1321 tgggccggtt cagagagtca ttgatggaac atggggcggt gttgaagaaa gagccgcggg
     1381 acgccaaacg agatgccaaa cgcgaaaaga atggtgatat cgagcacacg atgagagtga
     1441 ttaaggagac actctccagc ctcaaagatc aagagccggt gtctcgctaa gccggaaggg
     1501 gcggtagagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagagaca gtggcgttgc
     1561 ctaaaccgac agggccagat gatagacgga aaattgttgg tcgcggttcc atcccgctga
     1621 acgatagagc gcgtgggcgg cgtcgtttgt gagcgcagtt gagagcgaga ggcgcactgc
     1681 cccgagtgaa actgcaaagg tggcggcagc cgacaacagc gaagaagcca cgcgggttct
     1741 ccgggcctgc tcctgcacga aaagatcgtt gaggataaaa atcctggcca gcgacacgga
     1801 cgagaagctc ggatagagct gtgcaaatcc gatcgccgta tctccctcgt gcgcaatgaa
     1861 cagcgtggac tctttgttcg cacaacgggc aagcgcccgc gcggcagcca catcgctggt
     1921 ccgaccgtaa aactgacgat aggcgtcgaa gagtggaacg agttgttcga catcatcgat
     1981 ggtggcttgt ctgatggtat gtgtcataga tctccggcgg gcattgtgag cagcctgcta
     2041 gcccggatta ttcctgcatg ccgtctcggg cgccggagca ggtattcatg aatagtccgg
     2101 gctagaggac gcctcgttca atctcttcca gatagagaaa gtccggtgcc ttcactccgg
     2161 cagctcgcct caatttgacc aattccgggg actcgaagaa gcgccgggcg ttctccaagc
     2221 tagaccaggt cgagaagtgg acgacgcggt tggcatcctg gtcgtagcgc aggagttggt
     2281 agctgatttc cccggcacgt ttccgcagat ccgccgcgcc atcgaacacg gctttccagg
     2341 cggggtaggc ttcgacttcg tgaataatca acacgtgggg catgtgaact ccagaatgag
     2401 tggtgcggcc tagtgtactg caaaggcaga gggggattca tcacgatgga cgccaggggg
     2461 ccacgatgaa gaggctgtcg gagttggatg ggggagagtg gtggagcgat agccgtaatc
     2521 ggcgtcccac ttggcgagcg gcgcgttcat ggtcgtgagt acggattcgc agccgaacga
     2581 caggctgaag aagtcgatca tagctaacgc ggcggtctgc cgtccgattc gcggcgattg
     2641 atacttcggc taggttcttc gcttgagcat gtccgcgaga tgggcgaagg gcgtgaaggt
     2701 cgaaggttgg tcttgcttcc ggcgcggcgg cgtttcatcc gacgcatcag ctgcttgata
     2761 ccgctggtgc tcattgtcgt gacagtagag acagagcaac tcccagttgc tcccatcggg
     2821 cggattgttg tcatggttgt ggtccttgtg atggaccgtg agctgactca gcttcttgcc
     2881 gtcaaagtcg cgtccgcagt gcgcacagat ccacggaaat aatttgagcg cgcgctctcg
     2941 ataggtctgc tcccgctgag actggttgct ccgtgccacg cgagcctccc agagatgggc
     3001 gacacattac cgagatattt tacaacaagt cttggcctaa aacccgcgcc gccgcgaaat
     3061 ggtttttggc aacttctgac catccgcatt cacgtgctac cgtttttcag gcggtatggt
     3121 ttttatagcg gctgagaaca actcatgagc cgggagatca accggcctgt gtttgatggt
     3181 tgtgctcgcc gggtccttgc caagaaagtt gtcgcctttc cagttctttg acggcctgat
     3241 gggtctggga acgaaaccgc caccacagtt cgggcagaca ttgcctaaga c
//
LOCUS       DAORQN010000161         3267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_978_length_3267_cov_5.750311, whole genome shotgun sequence.
ACCESSION   DAORQN010000161 DAORQN010000000
VERSION     DAORQN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_3267_cov_5.750311"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3267)
                     /locus_tag="PK614_07900"
     CDS             complement(<1..>3267)
                     /locus_tag="PK614_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82171.1"
                     /translation="ITVSESNLNFWGRIKEYFSPTIGALSVYAIRPLADGAGEGRDYA
                     EHDGDIYTITIGENAPVILGDDPSRETGPHSSGVQLVSDTANVDAARPGIDHSRPTEL
                     AKFDPIDLFGEAPIILSKKTNQKDGEKEKVHTLEEDGVIQELSPQPSSPPVTLLTEYE
                     AIRNKVAAHTNEAQLGELSPTRLKSLSESLTIPLESLEQLTAAYKAATETRLPPEMFF
                     ALSRQGFASQMDELLDEDPKKFHKAIDRAIEAKQIPRLKDLKGVNQLIVMATERTPVA
                     TLLSHSGLSGSKKLQAALRHEGIRTLADLRRTGGLRALDNLSDSDRQAAAHLNAQSRL
                     LTISPDTRVTSALIKKGYTGIADIALAEPKELAAVLKDHVDAPSSEDMIGRAKAQYGL
                     VQSVSLGLSGSQATGFRTRDEWATNHPIFDMMTESCSCKDCDSAVSPRAYLADLMQYA
                     IDHLRESGRSVDLKYFETSFHQPFGKLPSSCVGMEEEVRQVRICIEVFRAYIGPRPIA
                     DVARESLLKDAEQRYRLEVYQVLLNKLGTNLTELRLAQGASIAQRKSLADRIAISDAA
                     LDAMYLDPTSAAKLTESALEGEFGLVDTTQPPLRNIATAIFTNARRSFIREAWKAQDW
                     PIDEYFERVVPIIDPDILGPDDFRLPYQKANAGDPDQAFDLWVARRDWADAFLESLRT
                     VPLRQNAGITGRDFAATVGAIGMAYRGRAALWRANALSEIKAKLTTLTPENSERIVQD
                     LKATYRLSLDAARRLIELWDKDAQFWRTPQRAPKVDESEWTEVFAILLKVVKTELSVF
                     WINEEIACAESRAQTNQIRRVWLNSQDASQALFGPREFWQSIGSSTDGEWPPIRVAGI
                     PLIDPELVKRDDLPEPVAGRRNPGLKDARTMWEERTTALNDKRRALVLELENADMNAV
                     LGSALGPIPAGAGVASWGEYLQALDDKLSGTNAGDMSSAKQIVEQTLHLTIEKFQRLV
                     TVIDAQSGVGGRPLPTAAELAEVFTMLINTWKLVDRYPAWSSEEIHVATGVEPWRCRR
                     HALSKWRATIEERQQWLRTLSVRSRPPLIDPDLLTSVGYLKTPGSGHAWTLWDQRQTA
                     "
BASE COUNT          737 a          889 c          842 g          799 t
ORIGIN      
        1 tcgctgtttg tcgttgatcc cacagagtcc atgcgtggcc ggatccaggg gtcttcagat
       61 aacccacact cgtcaagaga tctgggtcaa tcagtggagg gcgactacga accgagagag
      121 tacgcaacca ttgctggcgt tcttctatgg tggctctcca cttggaaagt gcatgccgtc
      181 gacaacgcca cggttccact ccagtcgcga catgaatttc ctcagaactc caggctggat
      241 accgatccac cagcttccac gtgttgataa gcatggtgaa cacttcagca agctcggcgg
      301 cagtgggtaa tggccgtcct ccaacacctg actgtgcgtc tatgacggtt accaaccgct
      361 gaaatttttc aatggtcaag tggagtgtct gctcgacaat ttgcttggca gaactcatat
      421 cgcccgcatt cgttccagag agcttgtcat caagcgcttg caagtactcg ccccaggatg
      481 cgacgccggc accagccgga atcggcccga gcgcgcttcc tagaacagca ttcatatcgg
      541 cattctctaa ttccaaaaca agcgctcgtc gtttatcatt cagtgccgtg gtgcgctcct
      601 cccacatcgt gcgagcatcc ttgaggccag gatttcttcg ccccgcaact ggctctggca
      661 ggtcatcgcg ttttacgagc tcggggtcaa ttagtggaat cccggcaacg cgaataggcg
      721 gccattctcc gtccgtcgag gagccgattg attgccaaaa ttcacgcggc ccgaacagcg
      781 cttgtgacgc atcctgagaa ttcaaccaga cgcggcgtat ttggttggtt tgtgcgcgag
      841 actccgcaca cgcaatctct tcattgatcc agaagacaga caattcagtc ttgacgacct
      901 tcaacagaat cgcaaaaacc tctgtccatt ccgattcatc gaccttagga gcgcgctggg
      961 gagttcgcca gaattgtgca tccttatccc acagctcgat aagacggcgc gcagcatcga
     1021 gagaaagccg ataggttgcc ttaagatcct ggactatacg ttcgctgttt tcaggcgtga
     1081 gagtcgtcaa tttggctttt atctctgaca aggcatttgc acgccagagc gctgcccgac
     1141 ctcgataggc catccctatg gctccgaccg tcgcagcaaa atcacgtccc gtgattccag
     1201 cattttgccg caaaggaact gtgcgaaggc tttcaagaaa agcatcggcc caatctcttc
     1261 gcgccaccca gaggtcgaat gcttggtccg gatcacctgc attcgctttt tggtacggaa
     1321 gacgaaagtc atcaggcccc agaatgtcag ggtcaatgat cggaaccact cgctcgaaat
     1381 actcatctat tggccaatcc tgagctttcc aagcttcgcg aataaaagat cgtcttgcat
     1441 tcgtgaatat ggccgtcgct atattgcgaa gtggaggttg cgtagtatcg acgagtccaa
     1501 actccccttc taatgctgac tctgtcagtt tcgcagcgga tgtgggatca agatacattg
     1561 cgtcaagggc cgcatcagaa attgcgatac ggtctgcgag ggattttcgt tgtgctattg
     1621 atgccccttg ggcaagccgc aattccgtga ggttcgtgcc aagtttgttc aagaggactt
     1681 gatagacttc cagtcgatac cgttgctctg cgtcctttaa caaggattcc ctggctacgt
     1741 ctgctatcgg tcgtggccca atgtacgcac gaaacacttc gatgcaaatg cgaacctgcc
     1801 gaacctcctc ctccatgccg acgcaactgg aaggcagttt tccgaaaggc tgatggaagc
     1861 ttgtctcaaa atacttcaga tcgaccgacc ggccgctttc ccgcagatga tcaatcgcat
     1921 attgcatcag gtcagcaagg tatgcgcggg ggctcaccgc cgaatcacaa tctttgcacg
     1981 agcagctctc cgtcatcata tcgaatatcg gatgattagt tgcccactcg tctctcgttc
     2041 gaaagcctgt tgcttggctc cccgacaacc ctaggctgac gctttgcacc aatccgtact
     2101 gagccttcgc tcgtccaatc atgtcttcag aagagggagc atcaacatgg tctttcaaca
     2161 ccgcggccaa ttcctttggc tcagcgagtg cgatgtcggc gatgccggtg tagcctttct
     2221 ttattaatgc ggatgtcacc cgcgtatcgg gagatattgt gagaagccga gactgcgcat
     2281 tcagatgggc ggcagcctgt cgatcggaat cgctcaaatt atcaagagca cgaaggcctc
     2341 cagtgcgacg tagatccgca agggttctga ttccttcatg tcgcagggca gcttggagtt
     2401 ttttggatcc ggacaagccg gaatggctca ggagggtggc aactggcgtg cgttcggtcg
     2461 ccatgactat caattgatta acacccttga gatccttaag ccgagggatt tgctttgctt
     2521 cgatcgcgcg atcgatggcc ttgtgaaact ttttggggtc ttcatccaaa agttcatcca
     2581 tttggcttgc aaatccttgg cggctcaacg caaagaacat ctccggtgga agacgggtct
     2641 cagtagctgc tttataggca gcagtgagct gttcaaggga ttcgagtgga atggtgagcg
     2701 actcagacaa actcttgagc cgggtcgggg aaagctcccc caactgggcc tcattcgtat
     2761 gcgcagcaac cttgtttcgg atcgcctcat actcagtgag aagggttaca ggcggagagg
     2821 aaggctgcgg ggataactcc tgtataacac cgtcctcttc aagggtgtga accttctcct
     2881 tttcaccgtc cttctggttt gtcttcttgg ataagattat aggcgcctct ccaaagaggt
     2941 ctatcgggtc aaatttagca agttcagtag ggcgggagtg gtcgattccc ggccgagcgg
     3001 catcaacgtt agccgtatca gaaacgagct gcactcctga tgagtgtggc ccggtctctc
     3061 ttgatggatc atcacccaga ataacaggcg cattctcacc aatcgtgatc gtgtagatat
     3121 ccccatcatg ctcggcgtag tctcggcctt ctccagcacc atccgctaac ggtcgaatgg
     3181 catagacaga gagagcacca atggtgggtg agaagtattc tttgattctc ccccagaaat
     3241 tgagattgct ctctgacact gtgatct
//
LOCUS       DAORQN010000162         3241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_982_length_3241_cov_3.774639, whole genome shotgun sequence.
ACCESSION   DAORQN010000162 DAORQN010000000
VERSION     DAORQN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_3241_cov_3.774639"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK614_07905"
     CDS             <1..448
                     /locus_tag="PK614_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82172.1"
                     /translation="RQSIALLKRLKAIGKVVKLKLYADAPLVKLVILGDYLWLQHYHA
                     DLDIQNMPEYVLQRNRKEHGLYTFYAHYFMQRWESPELPEYDLDTDELVYRSRTGQEI
                     RREPVEIGAAPVAAHSAETARVDTREAFQLLHAPGPYLPARGYDVE"
     gene            596..>843
                     /locus_tag="PK614_07910"
     CDS             596..>843
                     /locus_tag="PK614_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRB82173.1"
                     /translation="MGNGVERRAVSPMTTFFSYGFRPFFLGAALFAGVAVPAWILILA
                     GTGNPVLLSASRDWHVHEMLFGFLPAVIAGFLLTAVPN"
     assembly_gap    844..857
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <858..>1456
                     /locus_tag="PK614_07915"
     CDS             <858..>1456
                     /locus_tag="PK614_07915"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRB82174.1"
                     /translation="MLLFTLWLAGRAAMAIPLVVPIFAAIVDAAFLVALVGLLWRELA
                     AGNSWNHAPVAVAISLYAAANVTFHVLTVRGAETDLALRLALALDLFLLTFIGGRLTP
                     NFTREFLAGQGKKERPAPFSRFDALSIALVLMAALAWTVLPLASATGWIFLAAGTVNL
                     VRLLRWYGWLTWREPLVLSLHVGYGWLALSMLVLGCALLG"
     assembly_gap    1457..1522
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1523..1759
                     /locus_tag="PK614_07920"
     CDS             <1523..1759
                     /locus_tag="PK614_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRB82175.1"
                     /translation="AVMTRASLGHTGRPKQAGAATLCIYVLVTLGSILRVFGTSTGLP
                     TNLVLGSAAVGWSGAYLLFALVYGPFLLRPSLDE"
     gene            1970..2827
                     /locus_tag="PK614_07925"
     CDS             1970..2827
                     /locus_tag="PK614_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB82176.1"
                     /translation="MRATFRPGIREDAQACGAVFYEAFKLIAEQHNFPVDFPQPETAV
                     GLLTMLLSRDDVYSVVAEMERGIVGSNFLWESDAIAGVGPITVDPSVQNGSIGRHLME
                     RVLDRARAKNFVGVRLVQTAYHNRSLSLYTKLGFNAREPLSVMQGPAVQLQLPGYLVR
                     PASAGDLDACDQLCFHVHGHDRGRELFNATKQGTALVVEHGGGVTGYATLIGFFGHAV
                     GRTNEELKALIGAAPVFQGPGFLLPTRNGELFRWCLEHGLRVSYPMTLMSHGLYQEPS
                     GAFLPSVLY"
BASE COUNT          506 a          905 c          981 g          769 t
ORIGIN      
        1 tcggcagagc atcgcgttgc tcaagcgtct gaaggcgatc ggcaaggtcg tgaagctgaa
       61 gctctatgcc gatgctccgc tggtcaaact ggtgatcctc ggtgactatc tgtggctgca
      121 gcattaccat gccgatctgg atattcagaa tatgccggag tatgtcttgc agcggaaccg
      181 caaggagcac ggtttgtata cgttctacgc ccactacttc atgcagcgat gggagagccc
      241 cgagttgccc gaatacgatc tggataccga cgagctggtg tatcgcagtc gaaccggtca
      301 ggagatccgg cgggagccgg tggagatcgg ggccgctccg gttgccgcac attccgcgga
      361 aactgcgcgg gtcgatacgc gcgaggcgtt tcaattgctg cacgcgccgg gtccgtacct
      421 gccggctcgc gggtacgacg tcgagtaggg cagttgttct ttcattcctc accgcctgtt
      481 cagagcatcg gttgttctct gccgagacat tgcggtcgct gtggtattac tatggcggtc
      541 atgtcgacgc atcgatgtat cggcctgggt aggcgagggg gaggagtgag ggaccatggg
      601 taacggagtg gaacgacgcg cagtctcgcc gatgactacg ttcttttcgt acggattccg
      661 cccgtttttc ctgggggctg cactgttcgc cggtgtggcg gttcccgcct ggattctgat
      721 cctggctgga accgggaatc cggtcctgct ctccgcctct cgcgattggc atgtgcacga
      781 gatgctcttc ggttttctcc ctgcggtgat tgccggtttc ctcctcacgg cggtgcccaa
      841 ctgnnnnnnn nnnnnnnatg ctgcttttca ccctgtggct ggccggacgt gcggccatgg
      901 cgattcccct ggtggtaccg attttcgccg cgattgtgga tgcggcgttt ctcgtggcat
      961 tggtgggtct gttatggcgg gaacttgccg ccgggaacag ttggaaccat gcgccggtgg
     1021 ccgtggcgat cagtctgtac gccgccgcga atgtgacctt ccatgtcctc acagtgaggg
     1081 gtgcggagac ggaccttgcg ctgcggctcg cgcttgccct ggacctcttt ctgctgacct
     1141 tcatcggcgg acggctcacg ccgaacttta cgcgagaatt tctggcgggt caggggaaga
     1201 aagaacgacc tgcgccgttt tcccgcttcg acgcactctc gatcgcgctg gtcctcatgg
     1261 ccgcgctggc ctggacggtg ctgccgttgg cgtcggcgac gggctggatc tttctcgctg
     1321 ccgggaccgt gaatctcgtt cgtctcttgc gctggtacgg ctggctcaca tggcgggaac
     1381 cgctcgtgct gagcctgcat gtggggtacg gctggcttgc cttgtcgatg ctggttctcg
     1441 ggtgcgctct gctgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nngcggtgat gacccgcgcc agcctcggcc atacgggccg
     1561 tcctaaacag gccggtgccg cgacgctctg catctacgta ttggtcaccc ttggttcgat
     1621 cctgcgggtc ttcgggacga gcactgggct tccgaccaat ctggtgcttg gctcggctgc
     1681 ggttggctgg agcggcgcct atctgctctt cgccctggtc tatggaccgt ttctccttcg
     1741 tccgagtctc gacgagtgac gggtcgataa tcgtcggtga caggaccact cgcgtgcaat
     1801 cgactcagta gcgctttccg ctcggtgact catcccgtgc gctgatcctt ttctgtagac
     1861 caccgactat ccgggttgac agggttcatc tcgaaacgta ccctgtcctt ctggttggcc
     1921 cattcgagtg atcgggggtg acgggccggc caaaggagag gacctgtcca tgcgtgcgac
     1981 gtttcgtccg ggcatacgag aggacgcgca ggcctgtggc gccgtcttct atgaggcatt
     2041 caaactcatc gccgagcaac acaattttcc ggtcgatttc ccccagccgg agacggcggt
     2101 cggcctcctt acaatgttgc tctcacggga cgatgtgtat tccgtggtcg cagagatgga
     2161 acggggcatc gtcgggagca actttctctg ggaaagtgat gcgattgccg gtgtggggcc
     2221 gattacggtc gacccgtcgg tgcaaaatgg ttctatcggg cgtcacttga tggagcgggt
     2281 cttggatcgt gcacgcgcca agaactttgt cggtgtgcgg ctggtccaga ctgcctatca
     2341 caatcgctcg ctctccctct ataccaagct cggcttcaac gcgcgtgaac cgctctcggt
     2401 catgcagggt cccgcggtac agctccaact tcccggctat ctagtgcggc cggccagtgc
     2461 cggcgatctc gatgcctgcg atcagttgtg ttttcacgtg cacgggcacg atcggggcag
     2521 ggaattgttc aatgcgacca agcaagggac ggcgctcgtg gtcgaacatg gcgggggcgt
     2581 caccggttat gcgaccctga tcggattttt cgggcatgcc gtcggccgga cgaacgaaga
     2641 gctgaaggcg ttgatcggcg cggcgccggt attccaaggg ccaggctttt tgcttcccac
     2701 ccggaatggc gaattgttcc gttggtgtct tgagcatggc ttacgcgtca gctatccgat
     2761 gacgttgatg agccatggtc tctatcaaga accatccggc gcgttcctgc cgtcggttct
     2821 gtactgaacg tcatgtggcc gtcccagatc ccgtctactt gtggacggac gcacggcttt
     2881 ttcttgtcgg tgatccgtga taccgacgcc gaatccgaag gtggtcttcg tcaacgtccc
     2941 cttcgatgcc acgtatgaac ccctctttgt gacgttgggg gtgcttgtct gaaacccgta
     3001 tctctttcga taccgccggc tattcgtttg gatacagttc cctcgcctga gttacctttc
     3061 agaaaaatgc gtccattcgt atcgcctgaa tcagacaaca aagacttctc atgaatcgtt
     3121 ccggcacata ccttgctgtc tttcactgag tgcgtttcat cccgattgga tatggcaagt
     3181 ggtgtagcgc ggacttttcg caagtcggcg gattcgattt ccacacatct cggcattaac
     3241 t
//
LOCUS       DAORQN010000163         3238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_984_length_3238_cov_4.108388, whole genome shotgun sequence.
ACCESSION   DAORQN010000163 DAORQN010000000
VERSION     DAORQN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_984_length_3238_cov_4.108388"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PK614_07930"
     CDS             <1..923
                     /locus_tag="PK614_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRB82177.1"
                     /translation="KRLHIRVTRNYRQLLKMGPVDLVIDVSGNPEVGEYLQDIRRMGV
                     SVIGGASAKFMWQLIEARIRATADIEKALNKYQSLYRLYVKETGAAVTEERTRIACEI
                     HDGLVQSLAGVNFKLELSQELIRKNPKASLATIRESKAQLKLAIQEARQVIFNLRPLH
                     YDKMELIPALTNYLKSYETQYHIKTAFSVTGDETILFPRTKIFLFRIVQEALSNVQKH
                     AKAGRVSVQLDIRFDLLQVTISDNGIGFDMDAVLRDPEKWDHFGIRGILERARLVGGE
                     ATIDSKKGRGTRIVLRIPLADKETIRHGKN"
     gene            910..1581
                     /locus_tag="PK614_07935"
     CDS             910..1581
                     /locus_tag="PK614_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB82178.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKDDINVVGEAQDGVEAVEK
                     TKTLMPDVILMDVSMPRMGGIEATRQIKREFPHMGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMSQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANNLDLSEKTGKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LEPRP"
     gene            1865..2772
                     /gene="lpxC"
                     /locus_tag="PK614_07940"
                     /pseudo
     CDS             1865..2772
                     /gene="lpxC"
                     /locus_tag="PK614_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
     gene            complement(3015..>3238)
                     /locus_tag="PK614_07945"
     CDS             complement(3015..>3238)
                     /locus_tag="PK614_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRB82179.1"
                     /translation="GKSVPEAAYQILREASRPMHAKELLQRLLEGGLQIKGKTPLTSV
                     ATSLKRDKRFQKVGPNTFAVMTQELTAVV"
BASE COUNT          760 a          973 c          841 g          664 t
ORIGIN      
        1 ccaaacggct gcacatccgc gtaacgcgca actatcgcca actgctgaaa atggggccgg
       61 tcgatctggt catcgatgtg tcgggcaacc cggaggtcgg cgaatacctg caggacattc
      121 gccgcatggg cgtctcggtc atcggggggg ccagcgccaa attcatgtgg cagttgatcg
      181 aggcgcgcat ccgcgccacg gccgacatcg aaaaagcctt gaataagtac cagtcgctgt
      241 accgcttgta cgtcaaagaa accggcgccg cggtgacgga ggaacggacg cgcattgcct
      301 gcgaaattca cgacgggttg gtgcagagcc tcgccggcgt gaacttcaag ctggagctct
      361 ctcaagagct gattcggaag aatcccaaag ccagtctcgc cacgatccgt gaatccaaag
      421 cccaattgaa actggccatc caagaagcca ggcaggtcat cttcaacctg cgtccgctcc
      481 actacgacaa aatggagctc atcccggcac tgacgaacta tctcaagtcc tacgaaaccc
      541 aataccatat caagacggcc ttttccgtga ccggagacga aacgatcttg ttcccgcgca
      601 ccaagatctt cctgttccgg atcgtgcaag aagccctgag caacgtgcaa aagcacgcca
      661 aggccggccg ggtgtcggtg cagctcgata ttcggtttga tctgttgcaa gtgaccattt
      721 ccgacaacgg gatcggattc gacatggatg cggtccttcg cgatcccgaa aagtgggatc
      781 acttcggcat tcgcggcatc ctggaacgcg cacgactggt gggcggggaa gccacgatcg
      841 actcgaagaa agggcgcggc acgcgcatcg tgttgcgaat cccactagcc gacaaggaga
      901 cgattcgtca tggaaaaaat taaggtactg atcgctgatg atcaccgcgt ggtgcgcgaa
      961 ggcctggcgg ccatcctcaa gaccaaagac gacatcaacg tggtgggcga agcgcaagac
     1021 ggcgtggaag ccgtggagaa aaccaaaact ctcatgccgg acgtcattct catggatgtc
     1081 agcatgcccc gcatgggcgg catcgaagcc acccggcaaa tcaagcggga attccctcac
     1141 atgggcatcg tggcgctgac aatgtatgaa gagcagcaat acatcttcga tctcgtgcgc
     1201 gccggcgcca ccgggtatct gttgaaagac tctgagtcct cacaaatcgt ggcggccatc
     1261 cgggcgatct accggggcga gtcgcttatt catccgtcgg tcgccagtaa gatcctggcc
     1321 gagttctccc tcatgtccca gaagaagggc aagaagccgg cttgggttga acacgatttg
     1381 accgaacgcg aaatcacggt cctgcgcctc gtcgcagacg ggaagaccaa caaagaaatc
     1441 gccaacaact tggatctcag cgagaagacg gggaaaaacc acgtccgcaa tatctttcac
     1501 aaactgcagg tgtatgaccg gacccaggcc gcgattctgg ccatccgcaa agggctgatc
     1561 gaattggaac cgagacccta acggaggccg cgccaccaat gcaaagccca ggcacggatc
     1621 tcgatattga tgccggaata cgtggttccc gctcaatcca gcctgatata atcagttaac
     1681 tcattgaaaa ccaaacactt ccgtgcttcg cctcttccat caaaagatac aattgtgtga
     1741 atttcaccac agtgagcact ttcaaccatt caatataatt caatatatat caacgtctta
     1801 caattaattt aatgcttggc acaagaattg ctcatctggg agtacacatt acggaggagg
     1861 caccgtgcgg tttcagcaaa cgatcggatc accagtttca tgttcgggcg tcgggctcca
     1921 ctcaggccaa ccggtcacgc tgacactccg cccagctccc cctaataccg gaatcgtgtt
     1981 tgtctatcgc aacggatcgg aggaaaccct cctcccggct gcggtctcaa ataaagtacc
     2041 gaccgagctc tgcacggcca tcagcgtcaa cggccgtcaa gtgaagacca tcgaacacct
     2101 cctgtcggct ttggtcggta tggaagtcga caacgtgtac gcagaagtcg atgcagggga
     2161 agtccccgtg ctggatggcg attgattcgt gcggccggcg tgatccccca gacccgtcgc
     2221 caatcgtatg tgaaaatcac gcaacccatt gaggttgttg atggagcacg ccgagtcaga
     2281 atcgagccgt cttccacccc gaagatcacg tactccattc actacgatca tccattgatc
     2341 caaacccagt cctacaccta cgcctgctcc gcctcggctt ttgagcagga tattgcaacg
     2401 gctcgcacgt ttggattttt gcacgaagtc gaagccctgt gggccagagg cctcggaaaa
     2461 ggcgggacac tggacaacac cgtgattctg tccaaagacg gtgtcgtgaa tgaatcgggc
     2521 ctgcgcttcc agaacgagtt cgtccgccac aaggtcctgg acttgatcgg cgatatcgcg
     2581 cttctcggct tcccgttcat cggccatatc gttgcggaac gatcaggcca tgccatgcac
     2641 accagactcg ttgagcaaat cctggctcag cgcgacaaat gggccctcat caccggggaa
     2701 catgcagtcg cggcatccga atcccgctct tcgctcgggc tccttcgccc cgcaccatcc
     2761 ctcgcgatct aaccgctgct tctaagcacc cgcatacgca gatgcactcc tatgcctatg
     2821 ccccataagg catggctact tttaggcata aaaaaacgcc ggaggtaagt ggccttaccc
     2881 ccggcgtgta aatcctggtg ggacttactt cttcttcgct gccttcttcg ctggtttctt
     2941 cgctgccttc ttcgttgcca agactctcac ctcccttccc acagttagag ttgatatgcc
     3001 cctcttccgc ccgcttacac tactgccgtt aactcctggg tcatcaccgc aaacgtgttc
     3061 ggtccgactt tctggaatcg cttatcccgc tttaatgatg tcgccacaga cgtcaatggt
     3121 gtcttgccct tgatctgcaa accgccctcc aggaggcgct gcaagagctc cttggcatgc
     3181 atcggccggc tggcttcgcg gagaatctga taggcagcct ccgggacgct ctttccga
//
LOCUS       DAORQN010000164         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_985_length_3230_cov_10.385197, whole genome shotgun sequence.
ACCESSION   DAORQN010000164 DAORQN010000000
VERSION     DAORQN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_3230_cov_10.385197"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK614_07950"
     CDS             <1..253
                     /locus_tag="PK614_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB82180.1"
                     /translation="WAQINGRNALTWEQKFALDLWYVEHRSLRLDLHILIRTVWKVFT
                     RDGINQPGHATAEVYRRSSLISHTHTPMEAAHQKPSGDS"
     gene            432..1244
                     /locus_tag="PK614_07955"
     CDS             432..1244
                     /locus_tag="PK614_07955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB82181.1"
                     /translation="MNLLSLNLAETRWLTRVATGGWRKVRRSIKRRGILGTAGYMVCF
                     VPAKVARKLGFASPLAGPSVASLFDRKYGVETAEHIPLNRLGIESQNVNEGNYYAPTS
                     PKIFNEMMPHLNIRHEQFTFVDIGCGKGLVLLLASTYPFRRIIGVEFSAELSAIASNN
                     LAAYVGEHSKSREVHCLCMDAVDYEFPLEPLVIYFYNPFKGTVLERVAANIARTIADH
                     PRDLFVVYNNPTAPDVFDGIQSLERIFDDPDFLIYRTRLGARGRASSHGDKE"
     gene            1263..2165
                     /locus_tag="PK614_07960"
     CDS             1263..2165
                     /locus_tag="PK614_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82182.1"
                     /translation="MPGFLNAEEVVVNSMTEEQWAETLRRQGKHVISHRGRYWMELVA
                     GFYEPIHWLAALTENEATRPRFGCWGFRTRLKEAPPGLANGTMPVQLCSNVADYGEQS
                     LTPGRRNHLRRCRKRIRIVQLKDACLLRDQGRRVMLSAIRRTDYGHAPEQEAYCSEVD
                     VLVRDPHMVVLAGLAGDQLAGYMIGYAVGDVAYIQNIFLATEFLNTYVGTGLPFEFVL
                     ACRRTGMIHQVVYGLHSVEDPRLCTFKAGMGFPPVYLPSRVSLPPVVSTILRYRYPYK
                     FYRLTGQHPTVGEGASTSFDTGEC"
     gene            2302..>3230
                     /locus_tag="PK614_07965"
     CDS             2302..>3230
                     /locus_tag="PK614_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRB82183.1"
                     /translation="MITEFIQPADPRWRQHLETAPHDFYHLPEYVELCATYEGTTAAA
                     FYAKEGQSTFLAPLLIRSLPSELGVPCDWYDCVSPYGYPSPLVWPPQADLSPFLDAFC
                     QAARRRNLITAFFRLHPLLSLDHRALGRFGQLIRHGQTVCFDLTQSKEDLWNQVSTNH
                     QRNIKKLVRLGFRISIDEWSRLPDFVNLYHATMRRVDADDVYFFSREYFEELTAKLGD
                     RVHLACVLTERNEIAAAGLFVVTNGIVQYHLGGTAASHLSLAPSKLMVDYMWRWAQER
                     SHRVLHLGGGVGGTEDSLFHFKAGFSSARGEFYT"
BASE COUNT          656 a          889 c          927 g          758 t
ORIGIN      
        1 atgggcgcag atcaatggac gcaatgcatt gacctgggag cagaagtttg cgctggactt
       61 gtggtacgtc gagcatcgct cgctccgttt ggatctccac atcctgatca gaaccgtctg
      121 gaaagtattc acccgggacg gcatcaacca gcccggtcat gcgaccgcgg aagtctatag
      181 acgcagttct ctaatctctc acacgcatac ccccatggaa gcagcacacc agaagccaag
      241 tggcgactca tgagcagaga tgtagagaac ccaccggaaa tgtgaccact tcctttgcgc
      301 cgtgggtgtc gggcgcgtgc tccttgggcg ggagccgccc tgttgtcagt ccagattcag
      361 caagccgcat agacaatgtc tccttatcct acccacgaga cgcaagtcca atgtctgaac
      421 gggaagatgc tatgaacttg ttatcgctca atctagccga aacacgttgg ttgacgcggg
      481 tcgcaacagg cggctggcgt aaagttcgac gatccatcaa acgtcgcgga attttgggca
      541 cggcgggata tatggtctgc tttgtccctg ccaaggtcgc ccggaagctc ggcttcgcct
      601 ctccattggc cgggccgagc gtcgcaagtc tgttcgatcg caaatacggg gtggaaaccg
      661 cggagcacat tccgttgaat cgattaggca tcgagagcca gaatgtgaat gaaggaaatt
      721 attatgcgcc gacctcgccc aagatcttca atgagatgat gccgcatctg aacatccgcc
      781 acgaacagtt tacgttcgtc gatatcggat gcggtaaggg gcttgtgttg ctgctcgcat
      841 caacctatcc gtttcgccgc attatcggtg tggaattctc cgccgagttg tcggcgattg
      901 cttcaaataa cctcgccgcc tacgtcggag aacacagcaa atccagagaa gttcactgtc
      961 tctgcatgga tgcggtggac tacgagttcc cgctcgaacc tcttgtgatc tacttttata
     1021 atcccttcaa agggacggtg ctcgaacgcg tcgcagcaaa tatcgcccgt accattgccg
     1081 atcatccgcg cgatctcttt gttgtctaca acaatcccac ggcgccggac gtgttcgatg
     1141 gcatccagtc gctggagcgc atcttcgacg atcccgactt tctcatctat cggacaagat
     1201 tgggagcaag ggggagagcc tcatcgcacg gtgacaagga atagaaggag ttattgaacg
     1261 ccatgcctgg ctttcttaat gctgaagagg tagttgtcaa ttcgatgacg gaggagcagt
     1321 gggcggaaac cctgcgtcgc caagggaagc atgtgatctc tcaccggggg cggtattgga
     1381 tggaattggt ggccggattt tacgagccga ttcactggct ggctgccttg acggaaaacg
     1441 aagcgactcg gccaaggttc ggctgctggg gatttcgtac ccgcctgaag gaggcaccgc
     1501 cgggtttggc taacggaacc atgcctgttc agctgtgctc gaacgtcgcg gactatggcg
     1561 agcagtcgct gactccgggg cgtcgcaatc atctgcgccg ttgccgcaag cggatccgca
     1621 tcgtccagct gaaagatgcg tgccttcttc gtgaccaggg acgacgcgtg atgctgtccg
     1681 cgatccgccg aacggattat ggtcatgctc ctgaacagga ggcgtattgc tcggaggttg
     1741 atgtgctggt ccgagatccg cacatggtcg tattggccgg tcttgccggc gaccaactgg
     1801 ccggctacat gatcggctat gccgtcggcg atgtcgctta tatccagaac atttttttgg
     1861 cgaccgagtt cctgaacacc tatgttggga ccgggttacc cttcgagttc gtcctggcct
     1921 gtcggcgcac cgggatgatc catcaagtcg tgtatgggct gcatagtgtg gaggatccac
     1981 ggctatgtac gttcaaggcg ggaatggggt ttcctccggt ctacctgccg agtcgagttt
     2041 cgctcccgcc ggttgtgagc accattttga gatatcgcta tccctacaaa ttctatcgcc
     2101 tcaccggtca acatcctaca gtgggggagg gggcatccac gtctttcgat acgggagagt
     2161 gttgagaatt ccttcttcgt ccatggtcgc ggcacgatcg gtgggtgttt gtgtgaggac
     2221 acagtcagtg gacggccccc ggtgactcgc agcgagcctg ccatgtctgt ctgcaacgcg
     2281 tgttagagta tagaggttag cttgattacg gaattcatcc aaccggccga tcctcgatgg
     2341 cgacagcatc ttgagacagc gccgcacgat ttttaccatc tgcccgaata cgtcgaactg
     2401 tgcgcgacat atgagggcac gacggcggcg gcgttctacg cgaaagaggg gcaatcgacg
     2461 tttctggcgc cccttctgat tcgtagttta ccctcagagc ttggagtccc ttgtgattgg
     2521 tatgactgtg tctcgcctta cggctacccg tccccgctcg tctggccgcc gcaggcggac
     2581 ctgtctccct tcctcgacgc gttttgtcag gccgcacgac gccggaacct catcacggca
     2641 ttttttcgcc tccatccgct cttgtccctc gatcaccgcg ctctcgggag gtttggacaa
     2701 ctgatccgac acgggcagac tgtctgcttc gatctcaccc agtcgaagga ggatctttgg
     2761 aaccaagtct caacgaatca ccagcgcaat attaagaaac ttgtccggtt gggtttccgt
     2821 atcagtattg acgagtggag ccgactaccg gactttgtca atctgtatca cgccaccatg
     2881 cggcgggtag atgcggacga cgtctacttc ttttcgcgcg aatactttga ggagttgaca
     2941 gcgaagcttg gggaccgggt gcatcttgcg tgtgtactga ccgagaggaa cgaaatcgca
     3001 gccgcgggtc tcttcgttgt taccaatggg atcgtacagt accatctcgg gggcacggcg
     3061 gcctcacacc tctccctcgc tccttccaag ctcatggtcg attatatgtg gcgttgggct
     3121 caggaacgat cgcatcgcgt gttgcatctg ggtggagggg tcggcggcac cgaagactcg
     3181 ctatttcatt tcaaggctgg attttcctca gcacgcggtg agttctatac
//
LOCUS       DAORQN010000165         3199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_994_length_3199_cov_5.719466, whole genome shotgun sequence.
ACCESSION   DAORQN010000165 DAORQN010000000
VERSION     DAORQN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_3199_cov_5.719466"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PK614_07970"
     CDS             <1..737
                     /locus_tag="PK614_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRB82184.1"
                     /translation="GFRATETANQMIHYEEALGGEALGALAKQGTPRDRLFLQSKFTP
                     VNGQDHRTPYDAQADLTTQVTQSFASSLAHLHTDYLDSYVLHGPYGRRGLSDADWEVW
                     AAIEALYDAGKTKMIGISNVTADQLTLLCARATHKPMVVQNRCYAALGWDQDVRAICR
                     THGIIYQGFSLLTANRELFADPEVRAMAAKYGMGLAQLVFRFAMQTGMLPLTGTTDPQ
                     HMQEDLRSDQFTIAPDDLQRLEIIGM"
     gene            complement(843..1406)
                     /locus_tag="PK614_07975"
     CDS             complement(843..1406)
                     /locus_tag="PK614_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82185.1"
                     /translation="MGLIPELTWDRYHWHDLVPSGYRIALELRPGYFLGANQPRHEAR
                     LRYLQGIPLTPMTVLMINTVAEAVNNSRNPNHSVLMGSIAGVRGLSDNLYRNRAQTYM
                     NLEFRHAIQVAPRWALQGVLFSDFGAFQPFTEDGSQRDWIGTVNVGAGIRVVPTFLAN
                     TLLRVDFAQLLSPSPNSLIQVGITQYF"
     gene            1495..2703
                     /locus_tag="PK614_07980"
     CDS             1495..2703
                     /locus_tag="PK614_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82186.1"
                     /translation="MLHRIRTTSFQCLIGLVGLLAVLSLAGCLSPPTLNRAVLAYDES
                     ITDAISKQLLINIARAHHHQPVHFTGVSNIAATFDFRISAGATPALTGDAGKTLMPLF
                     GGSVAENPTISIAPIEGEDFTRRLLTPFQEGKFTLLLRQGGDIDLLLRLMAKELRLKS
                     NGEDMPYRNSPADKSGYEMFRKVVLHLSAIQDANKLYAESLLFERTWTIPGESVTAEG
                     FKALEQEYLVTYNPLQKTYTLRKPVSGRILITNYDPDTLPREERVRLHEEVDQRPMND
                     VAFDIRPGHYGGEWPLKGEFRLRSFNTMLNFLAQSIEEEPEYHVDKDVRTPPFLDNPS
                     KTLDLLVEGSSPSGSDLTVQSHGKYYAVNVTGPLARWNREAFKLLYQLFQMTVTEVSR
                     SGVPSITIAK"
     gene            2855..>3199
                     /locus_tag="PK614_07985"
     CDS             2855..>3199
                     /locus_tag="PK614_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82187.1"
                     /translation="MHRNVISRSLLWQWTAFFICLCLALPSWADEVHLQNGDRLTGTI
                     LKMEERVLTLQTDYGGEVKIDWGKVESLKSDGTLKILVPGESHDALRDFLYGAQALRE
                     TRELGPESPVPLA"
BASE COUNT          675 a          981 c          906 g          637 t
ORIGIN      
        1 cggggtttag ggcgaccgag acggccaatc agatgatcca ttacgaggaa gctttgggcg
       61 gagaggcgct gggggcgctg gcgaagcagg gcacgccgcg ggaccggctc tttctgcaaa
      121 gcaagttcac gccggtgaac ggtcaggacc atcgcacacc ctacgatgcg caggccgatc
      181 ttacgacgca ggtgacacaa tcgttcgcca gttccctcgc ccatctccac accgactatc
      241 tcgattccta tgtcttgcat ggcccctatg ggcggcgtgg tttgagcgat gcagattggg
      301 aggtctgggc cgccatcgaa gccctgtacg acgcggggaa gacgaagatg atcggcatca
      361 gcaacgtgac ggcagatcaa ctcaccctgc tttgtgcgcg ggcgacccat aagccgatgg
      421 tggtgcagaa tcgctgttat gcggcgctgg gctgggatca ggacgtgcgg gcaatctgcc
      481 ggactcacgg tatcatctac caaggcttct cgttgttgac tgccaaccgt gagctgtttg
      541 ccgatcccga ggtacgggcc atggccgcca agtacggcat ggggctggcg caactggtgt
      601 tccgatttgc catgcagacc ggcatgttgc cgctgaccgg tacgaccgat ccacaacaca
      661 tgcaggagga tctgcgctcc gaccaattca cgatcgcgcc cgacgatctc cagcgactgg
      721 aaatcatcgg gatgtgagca ggtttctgcc gctcgcctgt gtagcgcacg agctcttgcc
      781 tcccattcca aggtgagtga gatttcccat ttctggctgg aaatgaggcg ggcgctggtt
      841 cgtcagaaat attgcgtgat ccccacttga ataagcgaat tgggactggg ggacagcaac
      901 tgcgcgaaat ccactcgcag caacgtgttg gcgagaaacg tgggcaccac cctgatgcct
      961 gccccgacat tgaccgtgcc gatccaatcc ctctgactgc catcttcggt aaaaggttga
     1021 aaggcgccga aatctgagaa gagcactcct tgcagtgccc agcgtggtgc gacttgaatc
     1081 gcatgccgaa attccaggtt catatacgtc tgcgcccgat tgcgatagag attatcggag
     1141 agccctctga ctcccgcgat actccccatc aacacactgt gatttggatt gcggctgttg
     1201 ttcactgcct cggcgaccgt attgatcatc aggaccgtca tgggggtcag ggggattccc
     1261 tggaggtatc gaaggcgcgc ctcgtgacgc ggctggttcg cgccgaggaa gtaacccggt
     1321 cggagttcca gcgcgatgcg atacccggag ggcaccagat cgtgccaatg gtatcggtcc
     1381 caggtcagtt cgggaatgag acccacatag tagccatccg gtggccgccc ccctaggctt
     1441 ccttgaacgg gtgtgctaga aacgctggcg acgcacgacg ctcgggagta accgatgctg
     1501 caccgaatta gaacaacgtc attccagtgc ctgatcggcc tcgtcggtct cctggccgtt
     1561 ctcagcctcg caggctgcct gtcgccgccg acactcaacc gcgccgtcct cgcctatgat
     1621 gagtccatca cggatgccat ttccaagcaa ctgctcatca acatcgccag ggcccatcat
     1681 catcagccgg ttcactttac cggcgtctcg aacatagccg ccacattcga tttccgcatc
     1741 agtgccggag ccacaccggc attaaccggc gacgccggca aaaccttgat gccactcttc
     1801 ggcggaagtg tcgcggagaa tccgaccatc agcattgcac cgatcgaagg ggaagacttc
     1861 acgcggcgtc tcctgacccc gtttcaagaa ggcaaattca cgcttctcct ccgtcagggc
     1921 ggtgacatcg acctgctact tcgtctcatg gcaaaagagc ttcggctgaa aagcaacggc
     1981 gaggatatgc cctatcggaa cagccccgcc gataaaagcg gatacgagat gtttcggaag
     2041 gtcgtcctgc atctctccgc cattcaggat gccaataagc tctacgccga atcgctgctc
     2101 tttgaacgca cctggaccat tccgggggaa tccgtgacgg cagagggttt caaagccctg
     2161 gagcaggagt acctcgtcac ctataacccg ctgcagaaaa cctacaccct ccgcaaaccg
     2221 gtctccggga gaattctcat cacgaactac gacccggaca cgctcccgcg cgaggaacgc
     2281 gtcaggctgc acgaagaagt ggaccaacgc ccgatgaacg atgtggcgtt cgatattcgc
     2341 ccggggcact atggtggcga atggccgtta aaaggcgagt tccggctgcg aagcttcaac
     2401 accatgctca attttctcgc tcaatccatc gaggaagaac ccgaatacca cgtcgacaag
     2461 gatgtccgga caccgccgtt cctggacaat ccgtcgaaga ccctggatct cctcgtcgag
     2521 ggctcctcgc cgtccggatc ggacttgacg gtgcagtccc acggcaagta ttacgccgtg
     2581 aatgtcacgg gtccgctcgc ccgctggaac cgtgaagcgt tcaagttgct ctaccagctc
     2641 ttccaaatga ccgtcactga agtctcgcgc agcggtgtgc ccagcatcac catcgccaaa
     2701 taggcctcag tcggcagatc acccgtgcgc ggtttttttg ttcccgctct cttgcaactc
     2761 cgatggcgct ccagtagggt tggaccatcg acagaacgcg ggccgcccga ctctcgccac
     2821 gagtgacgta cccgtccgcc cctgattcac atccatgcac cgcaatgtca tcagtcgctc
     2881 cctgctgtgg cagtggacag cattcttcat ctgcctgtgt ctcgccttgc cttcctgggc
     2941 cgacgaagtt cacctgcaga atggagatcg actgaccggc acgattctca aaatggaaga
     3001 gcgcgtcctc accctgcaga ccgactacgg gggtgaggtg aaaatcgatt ggggcaaagt
     3061 cgagagcctg aagtccgacg gcacactgaa gattctggtg ccgggcgaat cacatgatgc
     3121 acttcgcgat tttctgtacg gcgcacaggc cctgcgtgag accagagaac tgggtcccga
     3181 gagtcccgtg cccttggcc
//
LOCUS       DAORQN010000166         3194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_997_length_3194_cov_19.923224, whole genome shotgun sequence.
ACCESSION   DAORQN010000166 DAORQN010000000
VERSION     DAORQN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_3194_cov_19.923224"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..874)
                     /locus_tag="PK614_07990"
     CDS             complement(338..874)
                     /locus_tag="PK614_07990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRB82188.1"
                     /translation="MNVPNSLTMLRILLIPVYVGLLNYEQFDYALATLFIAGLTDALD
                     GIIARVADQRTRLGEVLDPLADKLMLTTGFITLSVMHLVPLWLTILVVSRDLMLMLGA
                     AVAHFTHTQVDISPTVLGKGTTLVQLATLVAIIFFASRRLDLATLDPLLYLMGGVTLM
                     SGLHYLSRGYCRITSSQV"
     gene            1051..1371
                     /locus_tag="PK614_07995"
     CDS             1051..1371
                     /locus_tag="PK614_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82189.1"
                     /translation="MSGLPLLFKKEGLIERHQVEGIDPSDRYFNRAVLVSRVAAGYTG
                     KVTYEAYAVEGSAHSTTGAAVKAVVEKLMGVGFTRLRTRLNFKGNRYLAEKETWTDYP
                     DLPA"
     gene            complement(1368..2495)
                     /locus_tag="PK614_08000"
     CDS             complement(1368..2495)
                     /locus_tag="PK614_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRB82190.1"
                     /translation="MATTNPLQAALQAAVDDGAFPGAVLAVRLRGTLIYEGAVGRLSP
                     QIPGEGVTIHTCYDLASLTKVLATTTALLLLMQRGKLTLDDRIDRILIALQGSAAGAA
                     SIHQLLTHSSGLPGWRPYYERLASLEAGQSGFPGRTAAREAVLGYIAQEALVYERGSR
                     SLYSDLGFMLLGWAVERLAGESLDQFCNNQIYRPLEAQPLAYVPRESQAMPAASLESH
                     AIAPTEDDPWRGRMLCGEVHDENAFALGGVAGHAGLFGTARAVLAVAKAWMDGRRGKT
                     GLLQPDLVTLFTTRQQGIPNASWALGWDTPSAPSSSGTRFTPESFGHLGYTGTSLWID
                     PVKELEVVLLSNRVHPTRKNERIKTFRPLIHDLICREFLGD"
     gene            complement(2610..3122)
                     /locus_tag="PK614_08005"
     CDS             complement(2610..3122)
                     /locus_tag="PK614_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRB82191.1"
                     /translation="MKITPIDIQQMVFQVKFRGYDRDEVNRFLEELALTVENLNRENS
                     ALREKLTATEQQVTDLRRTETTLSNTLVSAQTLAEDVKRSAQRESDLIVKEAELKASE
                     IIRQARVSLSDMQRGLADLQKQRLMMVERFRSTLRSFERMLEVEESDAYQSDAASVEG
                     KLTGESSTAR"
BASE COUNT          718 a          982 c          894 g          600 t
ORIGIN      
        1 gccgctcccc aggctaaact tgaactttcc gcccatgccc gacttttctt ccaggctggt
       61 cacgacgacc tgctgcttgc gaagctggtc caccgcctca tctcgggtct tggcggtgag
      121 ctcccccttc ttcacagcac cctggcggtt ccgtccgaca taggcgaatg tactcataac
      181 gcggtttcca ctcctttgaa caccaggcag gatacgtacg gatgcagcgg aaaactatga
      241 tcgaagtcta taagacgacc taaaaactgt caaacgaatg cacgaaagcg gacccgcaga
      301 agaacatgaa agaagcggca cccaaagcgg cggcgcgcta tacctggctg gacgtgatgc
      361 ggcaatagcc gcgggagaga tagtgcaggc cggacatcag ggtcaccccg cccatgagat
      421 acaggagcgg gtcaagcgtg gcaagatcaa gacggcgaga ggcaaaaaag ataatggcca
      481 ccagcgtcgc gagctgaacc aaggtggtgc ccttccccag cacagtcggc gaaatatcga
      541 cctgggtatg ggtgaaatgg gcaacggccg cccccagcat caacatcaaa tcacggctga
      601 ccaccagaat cgtgagccaa agcgggacga gatgcatcac ggagagcgta ataaaccccg
      661 tggtcagcat gagtttatcc gccaggggat ccaggacttc tccgagtctg gtccgttgat
      721 cggccacacg cgcgatgatt ccgtcgagcg catccgtcaa tccggcaatg aagagcgtcg
      781 ccagcgcata gtcgaattgc tcatagttga gcaaccccac atacaccggg atcaacagga
      841 tgcgcagcat cgtcagactg ttgggaacgt tcatgttcat cgaaacagct ggtgtttgaa
      901 tcggcccctc gactggcgaa cgatgtcacg cgaatcgcgc atcatagaaa cccctggaga
      961 accttgtcaa cgacggttgc actccggaag ggaggtccct ataatcggga cgcgtaagac
     1021 gctcccagca gatgatggac ggaggtttgt atgagtggac tccccctcct ctttaagaaa
     1081 gagggcttaa tcgaacggca tcaagtcgag gggatcgacc cgagcgaccg atattttaat
     1141 cgagcggtgc tcgtcagtcg tgtggcggcg ggctatacgg ggaaagtcac ctatgaggcc
     1201 tatgctgtcg aaggctccgc tcactccacc acgggtgctg ccgtcaaggc cgtcgtcgag
     1261 aagttgatgg gagtcgggtt cacccgattg cgcactcgcc tgaacttcaa gggcaaccgc
     1321 tacctggccg aaaaagaaac ctggaccgac tatccggact tgccagccta atcccctaga
     1381 aactcgcggc aaatcagatc gtgaatgagc ggacggaatg tcttgatccg ttcatttttc
     1441 ctcgtcggat gtacccggtt ggacagcagc actacctcca attccttaac cggatcaatc
     1501 catagcgaag tcccggtata gccgagatgc ccgaatgatt cgggcgtgaa gcgcgttccg
     1561 gaagaagacg gggctgacgg cgtatcccac cccaaagccc agctggcatt cggtattccc
     1621 tgctgacggg tcgtaaacag ggtgacgagg tcaggctgta gcaatcccgt cttcccgcgc
     1681 cggccatcca tccacgcctt cgccaccgcg agtaccgcgc gcgccgtgcc gaacaacccg
     1741 gcatggccgg ccacaccgcc cagagcaaag gcattctcgt cgtgcacttc gccgcacaac
     1801 atgcgtcccc tccaaggatc gtcttcagtc ggagcgatcg catgtgattc caatgaggcg
     1861 gcaggcatgg cctgcgactc gcgcgggacg tacgcaagcg gctgtgcctc cagcgggcga
     1921 tagatctggt tgttgcaaaa ttggtccagc gactcacctg ccagccgctc caccgcccat
     1981 cccagcagca tgaaccccag gtcgctatac agactgcgag agcctcgttc atagaccaac
     2041 gcttcttgtg cgatataccc cagcactgcc tcgcgcgccg ccgtacgtcc cggaaatccg
     2101 gactgtccag cctcaagcga agccagccgc tcgtaataag gtcgccaccc gggaagccct
     2161 gagctatgcg tgagcaattg atgaatagag gccgcacccg ctgcactgcc ctgcagcgca
     2221 atcaagatgc gatcgatccg atcatccagg gtcagcttgc cgcgctgcat cagcaacagc
     2281 aacgccgtgg tggtcgccag caccttggtc aaagaggcca ggtcgtagca ggtgtggatc
     2341 gtcaccccct cgcccggaat ctgcggggac aggcggccga cggcaccttc atagatcaac
     2401 gttcctcgca atcggacagc caacaccgcg ccgggaaagg cgccgtcgtc gacggctgcc
     2461 tgtagggcag cctgcagggg atttgttgtg gccatgaatg cgcgcctacg tgtgatgcac
     2521 agggagatgg agggagaacg gaagggcgga gcccccttgg tagcccccgc tcgcaggcgg
     2581 agatggacgc ccaagggagt gcagaatgct caacgcgccg tgcttgattc gccggttaac
     2641 ttcccttcga ccgaagccgc atccgactga tacgcgtcgc tttcttccac ttccaacata
     2701 cgttcgaacg atcgcagggt tgaacggaat cgctccacca tcatcagacg ctgtttctgg
     2761 agatccgcga gaccacgttg catgtcggag agactcaccc gggcctgccg gatgatctca
     2821 ctcgccttca gctccgcttc cttcacaatc agatcggact cgcgctgcgc agaccgtttc
     2881 acatcctcag ccaacgtctg ggctgagacc aaggtattcg agagcgtcgt ctccgtgcgt
     2941 cgcaagtcgg tgacctgctg ctcggtcgcg gtcagctttt cacgaagcgc actgttctct
     3001 cgattcaagt tctcgacggt caatgccagc tcctcgagga accggttcac ttcgtcccgg
     3061 tcataacccc ggaacttgac ctgaaagacc atctgctgaa tgtcgatggg agtgattttc
     3121 atacgacacc tcttccccga cggttaatgc atccgcacgg cgatatcccg taacgattga
     3181 accacggcat attg
//
LOCUS       DAORQN010000167         3184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_998_length_3184_cov_9.116970, whole genome shotgun sequence.
ACCESSION   DAORQN010000167 DAORQN010000000
VERSION     DAORQN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_3184_cov_9.116970"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="gcvT"
                     /locus_tag="PK614_08010"
     CDS             complement(<1..1001)
                     /gene="gcvT"
                     /locus_tag="PK614_08010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRB82192.1"
                     /translation="MKQTPLIDAHRKAQGKLVDFAGWEMPIQYSGVVDEYQTVRRHAG
                     LFDVSHMGRISVSGPGSLAFLQRVTTNDVSKLSVRQSHYSMICAPNGGIKDDIFVYHV
                     KPYEFLVCVNASNREKIVAWLHEKVAQAQGCKVQDQSASLAQIAIQGPASRDILTAAG
                     LADLTTLKIRHCLDATLCGHSLLVTRTGYTGELGYELYLPAAGAPTVWDRLLEAGRPL
                     GIKPAGLGARDLLRLEMAYLLYGNDMNEETTPIEAGAEWVVKFDKGDFIGRTELLAQQ
                     SQGAARRLVAFELVEKAVPRHGFKILSAQVPHVEIGEVTSGNLSPLLQKGIGMGYVTP
                     "
     gene            complement(1057..1428)
                     /locus_tag="PK614_08015"
     CDS             complement(1057..1428)
                     /locus_tag="PK614_08015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB82193.1"
                     /translation="MAQSEQTASTPEITTVSGLTYTDVVVGTGREAAAGNLVTVHYTG
                     WLTNGKKFDSSVDRSEPFSFPLGAGKVIKGWDEGVAGMKVGGKRKLTIPSQLGYGARG
                     AGGVIPPNTTLVFDVELLEVR"
     gene            complement(1560..2786)
                     /locus_tag="PK614_08020"
     CDS             complement(1560..2786)
                     /locus_tag="PK614_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRB82194.1"
                     /translation="MILTPPSTLRFASALTRQADAQAAADELIRAIRVQLGSSRIDVA
                     FLFISAQHADQADTLSHALRTALGPDTLVGCTGEGVIATGREVETGPAATLWAAHLPG
                     VIAHPLRLSFSSVHDQFSLRDWPELDYGGESAPVMLLFADPFSTPLQDVLGVIEERYP
                     HARALGGLAGGGQDLAENRLFLDDEVYSDGLVGVALSGNISVRTVISQGCRPIGDRFI
                     VTKAEHNVIQELGGIPALHCLQTVFGQLSMDERAQAQRALHIGIAMDEQRAQFTRGDF
                     LIRNLLGADQQTGAIVVGDVIQEGQTVQFQVRDAQSADEDLHALLAASRLEESQRPLG
                     ALLFSCCGRGKGLFGVPNHDASVLGEQLGAIPLAGFFAQGELGPVGGRNFLHGYTASI
                     AIFSEPDRSDTQGSNH"
     gene            complement(2835..3062)
                     /locus_tag="PK614_08025"
     CDS             complement(2835..3062)
                     /locus_tag="PK614_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82195.1"
                     /translation="MADYPIGGGLTLLTALEKNGKFAEFLQTRMTQALETQDPTELHY
                     LLAQLDDYHSYMWRYYKKLASDRPERMNPGV"
BASE COUNT          653 a          899 c          951 g          681 t
ORIGIN      
        1 ggcgtgacat atcccatacc gatacctttt tgtagcaagg gcgacaagtt tccgctggtg
       61 acttcaccga tttcaacatg aggtacctgt gcactcagaa ttttgaatcc gtgacgcggc
      121 acagcctttt cgacgagctc gaacgccacg agccgtcgtg ctgctccctg tgattgttgg
      181 gcaagcaatt cagtccggcc gatgaagtct cccttatcga atttcacaac ccattccgcc
      241 ccggcctcga tgggagttgt ttcctcattc atgtcgttgc catagagcag gtaggccatt
      301 tcgagccgga gcaaatcacg tgcgcctaac cctgccggct taatccccag tggacgtccc
      361 gcttcgagta gtcgatccca gactgtcggc gcccccgctg ccggaagata caactcatat
      421 cccaactcgc cggtataccc ggtccgtgtc accaacagtg aatgaccaca gagcgtggcg
      481 tccaggcagt gacgaatttt cagcgtcgtg aggtctgcaa ggccggcggc tgtcaggatg
      541 tcacgtgaag ccggaccttg gatcgcgatt tgcgcgaggg acgctgactg gtcctggact
      601 ttgcagccct gggcctgcgc aaccttctcg tgcagccatg ccacaatctt ttcccggttc
      661 gaggcgttca cgcaaaccaa aaactcatac ggcttcacgt ggtagacgaa gatgtcgtct
      721 ttgatgccgc cgttcggggc acagatcatg gagtaatgcg attgacgcac ggacagtttt
      781 gacacatcgt tcgtggtgac gcgctgcaaa aatgccaacg agcccggtcc gctgacgctg
      841 atgcgcccca tgtgactcac atcgaacagt cctgcgtgcc ggcggacggt ctgatattcg
      901 tctactacgc cggaatattg gatgggcatt tcccagcctg caaagtcgac gagtttgccc
      961 tgggctttgc ggtgagcgtc gatgaggggt gtctgtttca tcaggtgcgt ctaacgtcca
     1021 cggtggtgag tgaatgcctg cgcgtgtaaa acggtcctac cggacttcca gcagttccac
     1081 gtcgaagacg agggtcgtat taggcgggat cacgccgccg gctccacgcg caccgtatcc
     1141 aagttgcgaa ggaatggtca gcttccgttt tccgccgact ttcattcccg cgacgccttc
     1201 atcccatccc ttgatgacct tgccggcacc gagtggaaag gagaacggct cgctgcggtc
     1261 aacggagcta tcgaattttt tcccgttggt cagccagccg gtatagtgga cggtcaccag
     1321 gtttcccgcg gcagcctcac ggcctgtgcc cactacgacg tctgtgtagg taaggcctga
     1381 aacggttgtg atttcaggcg ttgaggcggt ttgttcactc tgtgccatga ttggtctccc
     1441 tatggtcagg aataagaatc ccaagatacc gcaagtcgtc agccagaagc gagtgttcat
     1501 agaggtgtcc ttgtccagtc tgcaggtccg gtggcgtgac ctgcatgcgc tgacggtagt
     1561 caatgattcg agccttgtgt gtctgatcgg tccggttcag aaaagatggc gatgctcgcc
     1621 gtataaccgt ggagaaaatt acgtccgccg accggtccca actcgccctg cgcgaaaaat
     1681 cccgccagag gaatagcgcc caattgttcc cccaggacag acgcgtcatg gttgggaacc
     1741 ccgaagagtc ctttcccgcg tccgcaacaa ctgaacagca gcgcccccag tggccgttgt
     1801 gattcctcca gacgggaagc ggcgagtaac gcgtggagat cctcatctgc ggactgtgcg
     1861 tcccggactt gaaattgaac ggtttgtcct tcctggatga catcaccgac gacgatggcc
     1921 cccgtctgtt ggtctgctcc aagtagattg cggatcagaa aatcccctcg ggtaaattgc
     1981 gcgcgttgct catccatggc gatgccgata tggagggccc gttgggcttg cgcgcgttca
     2041 tccatggaga gttgcccgaa gacggtttgc aaacaatgca aggccgggat tccgccgagt
     2101 tcttgaatga cgttgtgctc cgcctttgtg acgatgaacc ggtctccgat cggacgacag
     2161 ccttgcgaaa taacggtgcg aaccgagatg tttccggaga gcgcgacacc gaccaagcca
     2221 tcgctgtaca cctcgtcatc cagaaaaagg cgattctcgg caagatcctg cccgccacct
     2281 gccagcccac cgagcgcacg agcatgcggg taacgctcct caatgacgcc caacacgtct
     2341 tgcagcgggg tggagaaggg atctgcgaag aggagcatga cgggggccga ctcgcctcca
     2401 tagtccagtt cgggccagtc acgcagtgaa aactgatcgt ggacgcttga aaacgacagt
     2461 cgtaacggat gtgcgatgac tccgggcaaa tgggccgccc agagggttgc ggccggtccc
     2521 gtttcgactt ctcgtccggt ggcaatgact ccctctcctg tacaaccgac caaggtgtcg
     2581 gggccgagtg ccgtgcggag tgcgtgcgag agcgtatcgg cctgatcggc atgttgggcg
     2641 gagatgaaca ggaaggcgac gtcgatgcgt gaggagccca actgcacgcg aatggctcgg
     2701 atcaattcat ccgccgcagc ctgcgcgtca gcctgacggg tgagggcgga ggcgaaccgc
     2761 agtgttgatg gaggtgtgag aatcacaggt ctgtctttgc caataggatc agggtcgtga
     2821 gctgtccggt gcggttagac acccggattc atgcgctcag gtcggtccga cgcgagtttc
     2881 ttataatagc gccacatata cgaatggtag tcatcgagct gggcgagcaa gtagtggagt
     2941 tcggtcgggt cctgggtttc cagtgcttgc gtcatgcgcg tctgtaaaaa ttcagcgaac
     3001 ttgccgttct tttctagcgc ggtcagaagt gtgaggccac ctccgatagg ataatcagcc
     3061 atagccctcc ggatgacgta ctgacaagcc aggagaatag ccgtggggcc ttgcaaaagc
     3121 aaggtatcga cggctcagcc ggggagatgg cgggctgtta gggcccgggc gtggcaccgg
     3181 cgga
//
LOCUS       DAORQN010000168         3154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1010_length_3154_cov_5.332688, whole genome shotgun sequence.
ACCESSION   DAORQN010000168 DAORQN010000000
VERSION     DAORQN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_3154_cov_5.332688"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK614_08030"
     CDS             <1..233
                     /locus_tag="PK614_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82196.1"
                     /translation="LGTVGGVKETALFGKGLHVVVERRAAGVIPAIKNRLVELNFAVS
                     RAERIAPSLEDVFVSLIEARDRQEHPQSEVSG"
     gene            complement(343..1020)
                     /locus_tag="PK614_08035"
     CDS             complement(343..1020)
                     /locus_tag="PK614_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRB82197.1"
                     /translation="MAHPKHLPAEQRRNDTVRALIALAAEQNPTDITTAAVAKRMGLT
                     QGALFRHFPSKDAILEAVMEWVAERLLARIDKAAQTASSPLTAVEAIFLTHIEFVIEH
                     PGVPRMLIGELQRSVDTVAKGMVRTLLRCYTERLIRLMEEGKKQGQLDAGLDSAAAAT
                     LFVGTIQGLVMQSLLTGNVSQIRRQAPGAFAIYRRGIASGKLPTQEGGVGHDHRHPVE
                     RKKWSDR"
     gene            complement(1202..>3154)
                     /locus_tag="PK614_08040"
     CDS             complement(1202..>3154)
                     /locus_tag="PK614_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB82198.1"
                     /translation="SLFAVDEVGNPTILATLTLIAALLPMAFVSGLMGPYMRPIPVNA
                     SIAMFVSLLVAFIVIPWFCQTCYRPGVHMAGVDHESFEGSRSYRLYQRLLAPVLSHPV
                     VAYLVLAVIALLLVGSVALFYTRAVVVKMLPFDNKSELQLVIDMPEGTTLEETARVTK
                     ALTQYVRTIPETRDYQAYVGTASPFNFNGLVRHYYLRDQPYEADIQINLVAKERRKTQ
                     SHDIAQRIRSAVQDIGRPFGANVKVLEVPPGPPVQSVLVAEIYGPDYERQRAIAREVR
                     GLFETTAGVVDVDDYLEADQVKYVFSVDRAKAALAGIPSEEIVRTLRLALEGADVGLV
                     HIPKENRPVQVRLRLPVIERTGLEHLGEISLRTTAGHMVPLSELIRVEQTVRDTAIYH
                     KNQKPVVYVIADVGGVGPDKGESPVYGVMGIGKKLGQLVLPEGYAMTQQYAVQPWSEQ
                     RFAMKWDGEWHITYETFRDMGIAFAVALLLIYLLIVAQFQSFLTPVVIMAPIPLTLIG
                     ILPGHWLTGSYFTATSMIGFIALAGIIVRNSILLVDFIQHQEAEGVSLLEAVIRAGAI
                     RTRPILLTAAALMVGAFVIILDPIFQGLAVSLLFGVGASTVLTLVVIPVLYYLWIGGP
                     RNSPAGLIPKQEHTELPEPVVGSRPG"
BASE COUNT          674 a          969 c          907 g          604 t
ORIGIN      
        1 cgctcgggac agtgggaggg gtcaaggaga cggccctatt cggcaagggg ctacatgtgg
       61 tggtcgaacg ccgggcggcg ggcgtgatcc cggcgatcaa aaaccggctg gtggaactga
      121 attttgccgt gtcgcgggcg gagcgcatcg cgccgtcgct ggaggatgtg tttgtgtcgt
      181 tgatcgaggc gcgggaccga caggagcatc cacaatctga ggtatcggga tgaacgcgcg
      241 gcgcatgtgg gcgcaactca gggggaggca acgacgactc agccctcacc aaactcatca
      301 tccagaatgt ggcgagtatt acgatgccca gcgttcgctt tatcatcggt cgctccattt
      361 tttacgttct acaggatgac ggtgatcgtg gccaacgccg ccttcctgtg tcggcaattt
      421 tcccgatgca atgccccgtc gatagatggc aaacgcacct ggcgcttggc ggcgaatctg
      481 tgacacattt cccgtgagca gcgactgcat aaccagcccc tgaatggtcc cgacaaacag
      541 cgttgcagca gcggctgaat cgaggcccgc atcgagctgg ccctgcttct tgccttcctc
      601 catgagtcga atcaatcgct cggtgtaaca acggagcaag gtccgcacca ttcccttggc
      661 gacggtgtct acggatcgct gcagttcacc gatcagcatc cgtggcaccc caggatgctc
      721 gatcacaaac tcgatatgcg tcagaaagat ggcttctaca gctgtgagcg gcgaggaggc
      781 agtttgcgcg gctttgtcga ttcgtgcgag caagcgttcc gccacccatt ccataaccgc
      841 ctccaagata gcgtctttgc ttggaaagtg cctgaacaga gcgccctggg tcagtcccat
      901 ccgcttggca acggcggcag tggtaatgtc ggtcggattc tgttccgccg ccaatgcgat
      961 caatgcccgc actgtgtcat ttcggcgttg ttccgctgga aggtgtttag ggtgagccat
     1021 acggcctcca tcaggataga aagtaattaa ttactatctt ttcgagtgtc atattgtcaa
     1081 tgggagcacc tgcggcatat ccaagacaga attaagcggc agcgtgtgag gcaggggtat
     1141 cttgctacaa ccccggccaa gagtctgtag cggaaagcgg cagaccggga tcagctccgg
     1201 ctcatcccgg tcggcttccc acgaccggct cgggcagctc tgtgtgctct tgtttcggta
     1261 tcaggccggc aggtgaattt cgagggccac cgatccacag gtagtagagc acgggaatga
     1321 cgaccagcgt cagaaccgtc gaggcgccga cgccgaacaa caacgagacg gccagtcctt
     1381 ggaaaatcgg gtcgagaata atcacgaacg ccccgaccat caatgccgcg gccgtcaaca
     1441 gaatgggacg ggtccggatc gcgcccgcgc gaatcacggc ctccaacagc gaaaccccct
     1501 ccgcctcctg atgttgaatg aaatcgacca gcagaatcga attgcgcacg ataataccgg
     1561 cgagggcgat aaacccgatc atcgacgtgg ccgtgaaata agagccggtg agccagtgac
     1621 cggggagaat gccgatgagc gtgagtggaa tgggcgccat gatgacgacc ggcgtcagaa
     1681 acgattggaa ctgcgccacg atcagcaaat agatcaacag cagcgccacc gcaaaggcga
     1741 tccccatgtc tcgaaatgtt tcataggtga tgtgccattc gccgtcccac ttcatcgcaa
     1801 accgctgttc cgaccagggc tgcacggcat actgctgcgt catcgcatac ccttccggca
     1861 acaccagctg ccccagcttc ttcccgatgc ccatcacacc atagaccgga ctctcgcctt
     1921 tgtccgggcc cactcctccc acatctgcaa tgacatacac caccggcttt tggttcttgt
     1981 gatagatcgc tgtatcccgc acggtctgtt ccactcgaat cagctcggac aacggcacca
     2041 tgtggccggc agtggttcgc aacgaaatct ccccgagatg ctccaaaccg gtccgttcga
     2101 tgaccggcag gcgtagtcgc acctgcaccg gccgattctc tttgggaatg tgcaccagtc
     2161 ctacgtcggc gccttccaat gccagtcgta acgtccggac gatttcctcc gacggaatcc
     2221 cggccagcgc cgccttcgcg cgatccacgc tgaacacata cttcacctgg tcggcttcga
     2281 ggtagtcatc gacatcgacg acgccggctg tcgtctcgaa cagtccacgg acctctcgcg
     2341 caatcgccct ctgccgctcg tagtcgggcc catagatctc cgccaccagc acagattgca
     2401 ccggcgggcc gggtggcacc tccagcactt tcacattcgc tccgaagggc cgcccgatgt
     2461 cctgcaccgc ggaacgaatc cgttgcgcga tgtcatggct ttgcgtcttc cgtcgttctt
     2521 tggcgaccag attgatttga atgtcggcct cgtacggttg gtcgcgcaga tagtagtgcc
     2581 gcaccagtcc gttaaaattg aacggcgagg ccgtgccgac ataggcctgg taatcgcggg
     2641 tctccggaat cgtgcgcacg tactgcgtca aagccttcgt cacccgggcc gtttcctcaa
     2701 gcgtcgtgcc ctccggcata tcgatcacca gctgcagctc gcttttgttg tcgaacggca
     2761 gcatcttcac caccaccgcc cgggtataga acagcgcaac cgacccgacc agcaagagcg
     2821 caatgaccgc caacaccagg taggccacga ccggatggga caacaccgga gccaggagcc
     2881 gctggtacag tcgataactg cggctgcctt cgaacgattc gtgatcgacc ccggccatgt
     2941 gcacaccggg ccggtagcag gtctggcaga accaggggat gacgatgaac gcgaccagta
     3001 gggagacgaa catggcaatc gacgcattca ccgggatggg ccgcatgtag ggccccatca
     3061 agcccgaaac aaacgccatg ggcaggagcg cggcaatcag cgtcagggtg gccagaatcg
     3121 tcgggttgcc gacctcgtcg acggcaaaca gcga
//
LOCUS       DAORQN010000169         3150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1012_length_3150_cov_7.233926, whole genome shotgun sequence.
ACCESSION   DAORQN010000169 DAORQN010000000
VERSION     DAORQN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_3150_cov_7.233926"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK614_08045"
     CDS             complement(<1..293)
                     /locus_tag="PK614_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82199.1"
                     /translation="MMHTTLLHHPRSRTPLLAALLLMGLLPSALAGEFRTDNPLNRPG
                     KIYWCPDRTADKRITATPDAGCTALYDEERDAKKPEKPDPSDHDTSTPKEPLK"
     gene            429..1793
                     /gene="asnS"
                     /locus_tag="PK614_08050"
     CDS             429..1793
                     /gene="asnS"
                     /locus_tag="PK614_08050"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRB82200.1"
                     /translation="MPVMYIDEAAAHAGQEVTIRGWLRSRRDKGKLHFLIVRDGTGDI
                     QAVVSKATVGDAQFARSAELTQESSLVLTGTLRQDARAPGGYELDVLRLEVLQVAEPF
                     PIQPKEHGTGFLMEHRHLWLRSSRQHAVLRIRHEIIRACRNFFDDRGFTLVDAPIFTP
                     NACEGTTTLFQTQYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAQLPDMMDLAEQFLSHIVAAVLKSRRAELLLLERDPAKLEHVAAPFP
                     RISYEEAIDILQRRGNPAKPGDDFGADDETILSNEFDRPVIVHRYPAALKAFYMETDP
                     ERPDLALCMDVLAPEGYGEIIGGGQRIHSHEKLLGRIQEHHLPVEAFQWYLDLRRFGS
                     VPHAGFGMGIERAGLGYVGSITCGKRFPFPGCCIDFIHSGESFRFCLHFGASDHRNAG
                     ISSI"
     gene            2026..2955
                     /gene="rsmH"
                     /locus_tag="PK614_08055"
     CDS             2026..2955
                     /gene="rsmH"
                     /locus_tag="PK614_08055"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRB82201.1"
                     /translation="MEIDSARDSHEPVLVEEILFWLQCKPGGVYVDCTLGYAGLAARL
                     LDRTAPDGILVGIDRDAAALAESQMRLREVAHRVHFRHGNFCDLKALIMGSGVSRVDG
                     VIFDLGVSSPQLDRAERGFSFREDGPLDMRMDQREGRTAADLVRDLPETELADLIYQL
                     GEERYSRRIARAIVQARMQGVIGTTWQLAAVVERAVPASYRHGRIHCATRTFQALRIA
                     VNRELDVLEPALRDAVDILAPGGRVCAVSFHSLEDRIVKHTFRSLANGSEASVRVLTK
                     KPVIASESERSRNPRSRSAKLRVVERISKEYVK"
     gene            2952..>3150
                     /locus_tag="PK614_08060"
     CDS             2952..>3150
                     /locus_tag="PK614_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82202.1"
                     /translation="MKAVAFAAVTSLVLLFVWERVDIVRIGYHIERLKAQKVLLERER
                     DELRVKISGLTAPERIARLASD"
BASE COUNT          604 a          798 c         1009 g          739 t
ORIGIN      
        1 atcttgagcg gctccttcgg agtggaggtg tcatgatcag acgggtccgg cttctccggt
       61 ttcttcgcgt cgcgttcctc atcgtacagg gccgtacagc ctgcatcggg agtggcggtg
      121 atccgcttgt ctgccgtgcg gtccgggcac caatagattt ttccgggccg gtttaacggg
      181 ttgtcggttc ggaattcacc tgccagtgcg gagggcaaca ggcccatcag gagaagggcg
      241 gcgagcagcg gtgtacgtga tcggggatgg tggagcaatg tggtgtgcat catgatgtga
      301 tgatgcccct ggcatgacat tgtgtcaacg aaagggggga tttgtgatgc gcttgaaaag
      361 cccgcgactg ttcgttaccc tccggccata gacctctcca gcgcacctct atttgaatag
      421 gatgacccat gcctgtgatg tatatcgatg aagcggccgc ccatgccgga caggaagtga
      481 cgattcgggg gtggctccgc agccgtcgag acaagggaaa gctccatttt ctgatcgtgc
      541 gggatggaac cggggacatt caggcggtcg tgagcaaagc gacggtgggt gatgcacagt
      601 ttgctcgatc ggcggaactc acccaggagt ccagtctcgt cctcaccggc acgttacggc
      661 aggatgcgcg tgcgccgggc gggtatgagc tcgacgtgct ccgtcttgaa gtgttgcagg
      721 tagcggagcc ctttcccatc caaccgaaag aacacggcac gggattcttg atggagcatc
      781 gccatctctg gttgcgctcc agccgacagc atgccgtgct tcgcattcgc catgaaatta
      841 ttcgagcctg ccggaacttt ttcgacgatc gtggttttac gctggtcgat gcgccgattt
      901 tcacaccgaa tgcctgtgag ggcacgacca ccttatttca aacgcagtac tttgatgaga
      961 cggcctattt gactcagagc gggcaactct atagtgaggc cacagccgcg gcgtttggaa
     1021 aggtgtattg cttcggtccg acctttcgcg cggagaagtc caagacgcgc cggcatttga
     1081 tggaattttg gatggtggag ccggaggtgg cctttgcgca attgcccgat atgatggatc
     1141 tggctgaaca gttcctgtct catattgtgg cggcggtgct gaagagtcgt cgcgccgagc
     1201 tgctgctgct tgaacgggat ccggcgaagc tggagcatgt ggcggcgccg tttccgcgca
     1261 ttagctacga agaggccatc gacattctgc agcggagagg aaatcccgcg aagcctggtg
     1321 acgattttgg tgccgatgac gagacgattc tttccaatga gttcgatcga ccggtcatcg
     1381 tgcatcgata ccctgcggcc ttgaaagcat tttatatgga gacggatccg gagcggcctg
     1441 atctggccct ctgtatggat gtgctggcgc cggaaggcta tggagaaatc atcggcggtg
     1501 gccaacgcat ccattctcac gaaaaacttt tgggacgcat tcaggagcac catctaccgg
     1561 ttgaggcgtt tcaatggtat ctcgaccttc gccggttcgg ctcggttccg catgcggggt
     1621 ttggaatggg tattgagcgt gcagggcttg gatatgtggg ctcgatcacg tgcgggaaac
     1681 gattcccttt ccccggatgt tgtatcgact ttatccatag cggcgagtcc ttccgtttct
     1741 gcctgcattt cggcgcgagc gatcaccgaa atgcaggtat ttcatccatc tgattttttg
     1801 atgatcccca tttccccctg acagaaactt gcccgaagat aattgtcagg tgggcgagtt
     1861 tcccacagac ttgtccacaa atgtggataa ctttcccgca aattccggcc aaattagaca
     1921 tgtgtctaaa ctgaacaata ttcttgatat ataggggtta attctgttga cagtcatttg
     1981 gttgggtgta tactccaccc aatgagtggg gtgaagtggg tggaaatgga aatagattct
     2041 gctcgcgatt cccatgagcc ggttttagtc gaagagatct tgttctggtt gcagtgtaaa
     2101 ccaggtggtg tttacgtgga ctgcactctt ggatatgcag ggctcgctgc tcgtctcctc
     2161 gatcgcaccg ctccagatgg catccttgtg gggattgatc gcgatgccgc ggcactcgcg
     2221 gagtcgcaaa tgcgtctgcg agaggtcgcg catcgggtgc atttccgaca cggaaatttc
     2281 tgcgacctga aagcgttgat catggggagc ggggtgtcgc gggtggatgg cgtgattttc
     2341 gatctgggcg tgtcctcgcc tcagctcgat cgtgcggagc gcggtttcag ttttcgggaa
     2401 gacggcccgc tggatatgcg catggatcag cgcgaaggac gcacggctgc agacctggtt
     2461 cgcgatctgc ccgagaccga gttggccgat ctgatctatc agctcggtga ggagcggtac
     2521 tcgcgcagaa ttgcccgtgc gatcgtgcag gcccggatgc agggggtgat cggaacaacc
     2581 tggcagttgg ctgcagtggt ggaacgcgcg gtgccggctt cgtatcggca cggacgaatt
     2641 cattgcgcca cgcgcacctt tcaggcgttg cggattgccg tgaatcgtga gttggacgtg
     2701 ttggagccgg cgctccggga tgcggtggac attctggcgc cgggcgggcg tgtctgtgcc
     2761 gtctcgtttc attcgctcga agacaggatc gtgaagcata cgtttcggtc gctggcgaac
     2821 ggctccgaag cgtcggtcag ggtgttgacg aaaaaaccgg tcatcgcctc ggaaagcgag
     2881 cgtagccgaa acccgcggtc acggagcgca aagttgcgag tcgtggagcg gatctccaag
     2941 gagtatgtga aatgaaagcc gtcgcgtttg ccgcggtcac gtcgctggtg ttgctctttg
     3001 tgtgggagcg ggtggacatc gttcgcatcg gctaccacat cgaacggctg aaggcgcaga
     3061 aggtcctcct tgagcgggag cgcgatgaat tgcgcgtgaa gatctccggc ctcacggcac
     3121 ctgagcggat tgcgcgtctg gccagcgaca
//
LOCUS       DAORQN010000170         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1015_length_3139_cov_6.930610, whole genome shotgun sequence.
ACCESSION   DAORQN010000170 DAORQN010000000
VERSION     DAORQN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1015_length_3139_cov_6.930610"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK614_08065"
     CDS             complement(<1..536)
                     /locus_tag="PK614_08065"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HRB82203.1"
                     /translation="MIRARITGTGSYAPERVMTNADLEKLVATSDEWIRERTGIRERR
                     IAAEGQACSDLGLIAAERALKAAGISAAELDMILLATCTGDMPLPSTACLLQHRLGAT
                     RAAACDVSAACCGFVYALGVADAYIRTGMRHILVVGSEVMSTITDWTDRNTCILFGDG
                     AGAAVVSASEGERGVLSP"
     gene            complement(591..1565)
                     /gene="plsX"
                     /locus_tag="PK614_08070"
     CDS             complement(591..1565)
                     /gene="plsX"
                     /locus_tag="PK614_08070"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRB82204.1"
                     /translation="MKIAVDAMGGDHGPAPVIEGAMQAAQELGVGIILVGKQDELAAA
                     CQKLNCTDTRITIHHAPQVVEMHESPATVARKKRDSSIWVATELVKSGEADAVVSPGN
                     TGASMVASFFVLGLIKGVERPAIATMLPTLTGSAVMLDVGANVDCTAQHLEQFALMGN
                     EFAKHVYAKQNPRIGLLSIGEEDSKGNEVTKEAFKLLKASPLNFIGNIEGREVYSGNA
                     DVVVCDGFIGNVALKISEGVADVIKKLLMKEISGSWLGRLAYPLIAKPLLNLKRKIDY
                     AEFGGGASARSEWDHYDLPWPFISQSDQECDSPREGLSRDQAGRIDPA"
     gene            complement(1717..1902)
                     /gene="rpmF"
                     /locus_tag="PK614_08075"
     CDS             complement(1717..1902)
                     /gene="rpmF"
                     /locus_tag="PK614_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRB82205.1"
                     /translation="MANPKHKHSRSRRDMRRTAKTRLVPPGFSLCPQCHELKLPHYTC
                     MNCGTYKGKAVIVVEES"
     gene            complement(1941..2612)
                     /locus_tag="PK614_08080"
     CDS             complement(1941..2612)
                     /locus_tag="PK614_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRB82206.1"
                     /translation="MSLGSSSRAVCLPLCSEAALPVMTVLMDTLKPAIVDIAPEGLSL
                     SCTATGPELGLDEPEEKCEGPLTIQLELVQHDGPITVTGTLEGTAIRQCVRCLTEYSD
                     PLFVNLYAEYLPQAGLATKPAPAEQGRRGPRRGAQPVEPVEEAEEADEVYLYQGEHLD
                     LVPMVREQVILAAPMQPLCREDCLGLCPQCGQNLNERRCGCPPEQGPSPFRVLRGRLS
                     KGGNA"
     gene            complement(2631..2888)
                     /gene="rpsR"
                     /locus_tag="PK614_08085"
     CDS             complement(2631..2888)
                     /gene="rpsR"
                     /locus_tag="PK614_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRB82207.1"
                     /translation="MERGDRGNGGGGGGRFFQRRKPCRFCVDKAPIDFKDVGLLRNFL
                     TERGRIVPRRISGNCLQHQRVLTVAVKRARQIALVSFAEER"
     gene            complement(2904..>3139)
                     /gene="ssb"
                     /locus_tag="PK614_08090"
     CDS             complement(2904..>3139)
                     /gene="ssb"
                     /locus_tag="PK614_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB82208.1"
                     /translation="IVVFGKQAEHCGQYLSKGNGVIIDGRLQQRRWETEDGQKRSKHE
                     VVAQGVTFLPKRGEAGGESGGLHEEPSYEDEQL"
BASE COUNT          612 a         1039 c          834 g          654 t
ORIGIN      
        1 tgaggggaga gcaccccgcg ctcaccttcc gatgcactca cgaccgccgc tccagcgccg
       61 tccccgaaca gaatacaggt gttgcgatcc gtccaatccg tgatcgtcga catcacttcc
      121 gaccctacga ccagaatatg ccgcataccg gttcgaatat aggcatcggc cacaccgagc
      181 gcgtagacga atccgcagca ggcggcagac acatcacaag ccgctgctcg ggtcgctcct
      241 agccggtgct ggagcaggca ggcagtggag ggtaacggca tatcccccgt gcaggtggcc
      301 aggaggatca tgtcgagttc agcggcggaa atgcctgcgg ctttcaatgc gcgttccgcg
      361 gcaatcaggc ccaagtcgga acaggcctgc ccctcggcgg caatgcgacg ttcgcgaatg
      421 cccgtgcgct cgcgaatcca ctcatcggac gtcgcgacca gcttctccaa atcggcattg
      481 gtcatcaccc gttcaggggc atacgacccg gttccggtga ttcgcgctcg tatcacgcct
      541 gcttcccttc cggtcgttcg tcctggtgcc gtctcaggct ttcttcaatg tcacgctgga
      601 tcaattcgtc cagcctggtc tcggctaagc ccttcgcgcg gcgaatcgca ttcttgatcg
      661 ctttggctga tgaacggcca tggcagatca tagtggtccc attcactccg agcagaggcg
      721 ccccccccga actcggcata atcgatcttc cgtttcagat tcagcaacgg cttcgcaatc
      781 agcggatagg ccaatcgccc gagccaggag ccggagatct ccttcatcag caacttcttg
      841 atgacgtccg caaccccttc ggagatcttg agcgccacat taccgatgaa tccatcgcag
      901 accaccacat cggcattgcc gctgtacact tcccgtccct cgatgttgcc gatgaaattc
      961 agcggactgg ccttcagcag tttaaaggct tctttggtga cctcgttgcc cttgctgtct
     1021 tcctcgccga tgctcaagag ccctatgcgc ggattctgct tggcgtacac gtgcttggcg
     1081 aactcgttcc ccatcagggc gaattgctca agatgctgtg ccgtgcagtc gacattcgcg
     1141 ccgacatcga gcatcaccgc gctgcccgtc aaggtgggca gcatggtggc gatcgccggg
     1201 cgttccacac ctttgatcaa tccgagcaca aaaaacgagg caaccatgct cgcgccggta
     1261 ttccccggac tcaccaccgc atcggcttca ccggatttca ccaactccgt tgccacccag
     1321 atggacgagt cacgcttctt ccgtgccacg gtcgccggcg actcgtgcat ctccaccacc
     1381 tgcggggcat ggtggatcgt gatccgcgta tcggtgcaat taagtttttg acatgcggcg
     1441 gccagctcat cctgcttgcc aaccagaatg atcccgacac ccagttcttg cgcggcttgc
     1501 atcgcgcctt caatgaccgg ggccggtccg tggtctccgc ccatcgcatc gacggcaatc
     1561 ttcatgtgcg gctagcggtc cacggataga gatgtgaaga atcgtgtaga gacgccttgc
     1621 cggctaggac ctgaacggcc tcacgcatga catagcgtcg gatcgtgatc acggtgcgca
     1681 attgtacccg aatcacgtct ggaaaataaa actcggctag ctttcctcga cgacgatgac
     1741 ggccttccct ttgtaggtcc cgcaattcat gcacgtatag tgcggcagct ttaattcgtg
     1801 acattgcggg cacagcgaaa acccaggggg aaccagcctg gtcttggccg ttcgccgcat
     1861 atctcgccgg gaccgtgaat gtttgtgttt tggatttgcc atgacgactc cttacaacgg
     1921 gtacatgccc ggttaacaga ctatgcgttt ccacccttgg ataggcgccc ccgcagcaca
     1981 cgaaagggac tcggcccttg ctccggcggg cagccacagc gacgctcatt gagattctgg
     2041 ccgcactgcg gacagagacc aaggcaatct tcccgacata acggctgcat cggagcagcc
     2101 aggatcacct gctcgcgcac cattggcacc aggtcgaggt gttcgccctg atacagatag
     2161 acttcgtccg cctcttccgc ctcttcaaca ggctcaaccg gctgggcacc gcgtctcggc
     2221 cctctccggc cctgttccgc cggagccggc ttggtcgcca ggcccgcctg cggaagatac
     2281 tcagcataga gattcacaaa gagcggatcg gaatattccg tgaggcagcg gacacactga
     2341 cggatcgcgg tcccttccaa cgtgccggtc accgtgatcg gaccgtcgtg ttggacaagt
     2401 tccagttgaa tcgtaagcgg cccctcacac ttctcttccg gctcatccaa gccgagttca
     2461 ggccccgtgg ccgtacacga cagagacagt ccttctgggg caatatcgac aatcgctggc
     2521 ttgagcgtat ccatcagcac cgtcatgacg ggaagagccg cctccgaaca gagcgggaga
     2581 cagactgccc ggctacttga tccaagactc accaaaacct atccctcgcc ctatcgctct
     2641 tcagcgaaac taaccaaggc gatctggcgc gcccgcttta ccgccaccgt caacacgcgc
     2701 tggtgctgca ggcaatttcc ggaaatgcgg cgtgggacga ttcgtccgcg ctcagtcaga
     2761 aaattccgca acaggccgac atccttgaag tcaatcggcg ccttatcgac acaaaaccgg
     2821 cacggcttgc ggcgctgaaa aaaccgaccg cctccgcccc cgccattccc gcgatctcct
     2881 cgttccatac tcgcctccgt ctgctacagc tgttcgtctt cgtaagaagg ttcttcgtgt
     2941 aggccgccgc tctcgcctcc tgcctcgccc cgcttcggta ggaaggtcac gccttgggcc
     3001 acgacttcat gtttgctgcg tttctgtccg tcttcggtct cccagcggcg ctgctggagc
     3061 cggccatcga taatgacgcc gttgcccttg ctgagatact gcccgcaatg ttccgcctgt
     3121 ttcccgaaca ccacgatgt
//
LOCUS       DAORQN010000171         3123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1022_length_3123_cov_11.714798, whole genome shotgun sequence.
ACCESSION   DAORQN010000171 DAORQN010000000
VERSION     DAORQN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1022_length_3123_cov_11.714798"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1347
                     /gene="alaC"
                     /locus_tag="PK614_08095"
     CDS             151..1347
                     /gene="alaC"
                     /locus_tag="PK614_08095"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HRB82209.1"
                     /translation="MAIGDGFYRIKRLPPYVFAQVQTLKLEARQRGEDIIDFGMGNPD
                     QPTPPHIVDKLIEASRKAKNHRYSASRGITKLRHAITGWYKRNYDVDLDPETEAIVTI
                     GSKEGIAHLALATIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRQDSDFFDDLLR
                     VYRQTLPRPRILVINFPHNPTTAVVDLEFFKKIVAFAKEHDVIVIHDLAYADIAFDGY
                     KAPSFLQVPEAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKLKSYLDYGIFQ
                     PIQIASTVALNGPQDCVKEVVQRYQNRRNVLVNGLNRIGWPVALPRATMFVWARIPDP
                     FRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEFVRFALVENEHRTRQALRGIRKVLN
                     LDDQES"
     gene            1344..2657
                     /locus_tag="PK614_08100"
     CDS             1344..2657
                     /locus_tag="PK614_08100"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRB82210.1"
                     /translation="MKSEIGVGLIGFGTVGTGVARVLIENAELIRRRVGVPVRLVRIA
                     DLDITRDRGIAIPPGVLTTDIQQVLTDPRVDIVIELMGGYDLAKRVILDAVQRGKHVV
                     TANKALLAVHGEEIFAAASRQGIDLGFEASVGGGIPVIRALMEGLAANNIQSIYGIIN
                     GTSNYILSRMTSEGQRFDVVLEEAKRAGYAEADPTFDVAGTDSAHKLTIMVSLAYGTP
                     VNFKEIYTEGITGLTPLDIAYAKEFGFTIKLLAIAKYSDGEIEARVHPTMVPSASQIA
                     KVDGVYNAIQLVGDAVEDVVLYGRGAGSMPTGSAVVSDVMAIARNLLKDAAGRVPPAS
                     YQPDQRRPLRMRPMEEITSLYYIRFMVVDRPGVLSQIAGVLGRYGISISSVLQQGRKE
                     GQTVPVVIMTHMAKERDIQNALREINPMSYISEPTTLIRVEGRDE"
     gene            2703..>3123
                     /locus_tag="PK614_08105"
     CDS             2703..>3123
                     /locus_tag="PK614_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRB82211.1"
                     /translation="MNRWRGIIEEYRKFLPVTAQTPVITLGEGNTPLIRAARLAKKIA
                     PGIDLYLKYEGMNPTGSFKDRGMTMAISKAVEGGARAVICASTGNTSASAAAYGARAG
                     IAVYVLIPAGKIALGKLSQAMMHQATVIQVEGNFDQAL"
BASE COUNT          655 a          816 c          977 g          675 t
ORIGIN      
        1 tgtgaccacg atagggtgct cgcccccaag tcctgacaat cgtagcgaaa ttgactcgtt
       61 ttgaggcctc cattaaaata cgcccctttc agaggcatgg cgactgaacc tgttcggcag
      121 aggacccgcc tgtaggagtc tgacaaattc atggcgatcg gcgacggctt ttaccgcatt
      181 aaacgacttc ccccctacgt atttgcgcaa gtacaaaccc tgaaacttga ggcgcgtcag
      241 cggggcgagg atatcatcga cttcggcatg ggcaacccgg atcagcccac gcctcctcat
      301 atcgtcgata aattgatcga agcatcgagg aaggccaaaa accatcgtta ttcagcttca
      361 cgcgggatca cgaagctgcg ccatgcgatt accggttggt ataagcgcaa ttacgacgtg
      421 gatttggatc ctgagaccga agcgatcgtg acgatcggct ccaaggaagg catcgcccac
      481 ttggcgctgg ccacgatcgg gccaggcgat gtggtgttga cgcccacgcc cacctatccc
      541 atccatatgt acagttttat catcgccggc ggggaagtgc gcgggatcga gctgcgccag
      601 gacagcgact tttttgatga cctgttgcgc gtctatcgcc aaaccttgcc gcgtccgcgg
      661 attctcgtga tcaatttccc gcacaacccg acgaccgccg tggtcgatct tgagtttttc
      721 aagaagatcg tcgcgtttgc caaggaacac gacgtgatcg tgattcacga cctggcctat
      781 gccgatatcg cgttcgacgg ctataaggcg ccgagctttc tgcaagtgcc ggaggcgaag
      841 gatgtgggtg tggagtttta tacgctgtcc aaggcctaca acatgcccgg ctggagagtc
      901 ggattctgtg tcggcaaccg tgaagtggtc ggcgcgctcg ccaagctgaa gagttatctc
      961 gactacggaa tttttcagcc gattcagatt gccagtaccg tcgcgttgaa tgggccacag
     1021 gactgcgtta aggaagtcgt gcaacgatat caaaaccggc gcaatgttct cgtgaacgga
     1081 ctgaatcgta tcggctggcc ggtggcgtta ccccgcgcga ccatgttcgt gtgggcccgt
     1141 attccggacc cgttccggca tatggggtcg ctggagtttt cgaagctgct cctccgggaa
     1201 gccaaggtcg ccgtatcgcc aggaatcggt ttcggagaag gcggagacga attcgtgcgg
     1261 tttgcgctgg tggaaaatga gcatcgcacc cggcaggctt tgcgcgggat ccgcaaagta
     1321 ttgaatttgg atgaccagga atcatgaaga gtgagatcgg ggtcgggtta atcggattcg
     1381 gaacggtcgg caccggtgtg gctcgtgtgt tgatcgagaa tgctgaattg attcgtcggc
     1441 gggtgggtgt acccgtcagg ctggttcgca ttgcggacct ggacatcacg cgcgatcgcg
     1501 gcattgcgat tccgccgggc gtgctcacga cggatatcca gcaagtcctc acggatccac
     1561 gggtggatat cgtcatcgag ttgatgggcg ggtacgatct cgccaagcgt gtgattttgg
     1621 acgccgtgca acgaggcaaa catgtggtca cggctaacaa agccttgctg gccgtgcatg
     1681 gcgaggagat ttttgccgcg gcttcgcgtc aaggcatcga tctcggcttc gaggcgagcg
     1741 tggggggtgg aattccggtc atccgcgcat tgatggaagg attggccgcg aacaatatcc
     1801 agtcgatcta cggcatcatc aacggcacct cgaattatat tcttagccgg atgaccagtg
     1861 aaggccagcg ttttgacgtg gtgttggagg aggcgaaacg tgccggctat gcagaggccg
     1921 atcccacgtt cgatgtggcg ggcactgatt cggcgcacaa actgaccatc atggtcagtt
     1981 tggcctatgg gacacccgtc aatttcaaag agatctacac cgaaggtatc accgggctca
     2041 cgccgctcga tattgcttat gcgaaggagt tcgggtttac aatcaagctg ctggcgatcg
     2101 caaaatattc cgatggcgaa atcgaggcgc gcgtgcatcc gacgatggtg ccgtcggcct
     2161 ctcaaatcgc gaaggtcgac ggtgtctaca atgcgattca gctggtgggc gatgccgtcg
     2221 aagatgtcgt gctgtatggc cggggcgccg ggtcgatgcc gacgggaagt gcggtcgtga
     2281 gtgatgtcat ggccatcgcg cgcaatctgc tcaaggatgc cgccggtcgt gtcccgcctg
     2341 catcctacca gcccgatcag cggcggccgt tgcgcatgcg accaatggaa gaaattacct
     2401 cgctctacta cattcgcttt atggtggtcg accggccggg agtgttgtcg cagattgccg
     2461 gggtgttggg tcgttatggg atcagcattt cgtcggtgct gcagcaggga cgcaaagagg
     2521 ggcagacggt gccggtggtg atcatgacgc atatggcaaa agagcgggat attcagaacg
     2581 cgctccgtga aatcaatccg atgtcctaca tctccgagcc cacgacgctg atcagagtcg
     2641 aagggcgtga tgaatgaatg tgtctctccg gttgtgctga gaggatggtc gaatagattg
     2701 ttatgaatcg ttggcgtggg atcatcgaag aatatcggaa gttcctgccg gtcacggcgc
     2761 agacccctgt gatcacactg ggcgaaggca atacgccact gattcgcgcg gcgcggctcg
     2821 cgaagaagat cgctccgggg atcgatctgt atctgaagta tgaaggcatg aatcccaccg
     2881 gatccttcaa ggatcgtggc atgaccatgg cgatctccaa ggctgtcgag gggggcgcgc
     2941 gggcggtcat atgcgcctcc accggaaaca catctgcctc cgctgcggcg tacggggcca
     3001 gggcgggcat tgccgtgtat gtgctcattc cggccggcaa gattgcgctg gggaagttgt
     3061 cgcaggcgat gatgcaccag gccaccgtca ttcaggtcga aggaaatttt gaccaggcgc
     3121 tga
//
LOCUS       DAORQN010000172         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1023_length_3121_cov_4.606654, whole genome shotgun sequence.
ACCESSION   DAORQN010000172 DAORQN010000000
VERSION     DAORQN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_3121_cov_4.606654"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            633..2600
                     /locus_tag="PK614_08110"
     CDS             633..2600
                     /locus_tag="PK614_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82212.1"
                     /translation="MNNATSRFSLPAVALLIAASLIPRPAQAACAAPVAVAGTLEYFS
                     AQNIFKYCDGTNWIGWGDGGSASSLALSVVTAATGANTIASGVNAQIWNWALTGATAD
                     AFTFGETTAATGGSGDQSVLKAAAIASSTAIPLMVTNLRVNDATGDADTTPFVVDASG
                     NVGIGTATPGSKLHIKDTGGAGTYRDLATIEANGEPWLTLSGADADQWYNPTLLTKRA
                     RGSLSAPAAVQDDDELFCIDAFGYDGTGYISTSGICSYVDGAVSAGNIPARWGWWTIP
                     GVTQPNMILDSVGKLTIWGINADTVKQLMSLQTTGEGALNISNASDFDYFNPIIFTNR
                     ARGTIGARTAVASADSAFEILVHGYNGTTDLPLAYIQALVDGTPGTNDMPGRIEFHTQ
                     ADGAGGSLEGTTPEMVIKNNGNVGIGTATPTGLLEVASSTVEDLLILDVANDIAGTDP
                     SILLRRSRGTLSARTTVSSGDNLGAIWASGYDGSNMEYAASIEFEVDGGVATAGDTTD
                     MPGAIRFKTTPDGSNVSVERMRIDDAGNVGIGTTSPASKFVVAPPASQAIAGADTITA
                     DACGTIKQITSAGNVTTNTTNTLTAPTASYAGCCMTVINVDTADTITLDANTLFKTIG
                     GANQALGPSDTVQVCSNGTNWYQVGAVSGNQ"
BASE COUNT          694 a          899 c          848 g          680 t
ORIGIN      
        1 cgttggcggt gtctcttccc aagcatgcag tcgagagagg agtgccagct ttactgtgtg
       61 agatttcgtt gcaaccgaga aatgaagccg cacttgctgc tgcaaacagg actgggttcc
      121 gaatggtagc aacgaggacg aagtataacc ctgccgaaaa gaaggatcgc gtttcaggga
      181 catttatgcg aattctcgaa gacaaggcat ggcgatagat cgctggttca tgttaaagac
      241 gagtttatat ctatcgctga aagaccccct ttctgcacgt gcgttttctg attgattgcc
      301 tctcggagtc gcttcaattc ctgcgccaag gccttgcctg aacatccaaa cttttcggcc
      361 atggcggtga tcgttgcttc ccctgcttta atcctggaat acgccagaat cactttctac
      421 aggatgggtt tgcgtggcct gtcgatatgc ttgccgctcg gcttggcctg ggccattcca
      481 gtcatggtta gatagacctc ccccgcttaa acagcgttct tgagcaatac acgtattgct
      541 ggtagcatcg cattgccagt gtggcggtgt acgtgctgca cttcgggtaa gggataaggc
      601 catcctttcc cgcttgcaga ggatcaaccg ccatgaacaa tgccaccagc cgcttttctc
      661 ttccagccgt cgcgctcctg attgccgcat cgcttatccc gcgccccgcg caggccgctt
      721 gcgctgctcc ggtcgctgtt gcaggcactc ttgagtattt ctccgcccag aacattttta
      781 agtattgcga cgggacgaac tggatcggct ggggcgatgg tggctctgcc agcagccttg
      841 ctctgtccgt cgtcacggcg gccaccggcg cgaacacgat tgcaagcggc gtcaacgcgc
      901 aaatatggaa ctgggcgctg acgggcgcga cggcggacgc cttcaccttc ggcgaaacca
      961 cggcggccac cggcggcagc ggagatcaat ccgtcctgaa agccgccgcc atcgcctcat
     1021 cgacggccat cccgctgatg gtcaccaatc tccgcgtgaa cgacgccaca ggcgatgcgg
     1081 acaccacgcc gtttgtagtc gatgcgtcgg gcaatgtcgg catcggaacg gctacgccgg
     1141 gcagcaagct gcacatcaag gataccggcg gcgcaggaac ctaccgcgac ctagccacca
     1201 tcgaggccaa cggcgagccc tggctcacac tcagcggcgc ggatgccgat caatggtaca
     1261 accccacgct tttaaccaaa agagcgcgcg gtagcctttc cgctccggcg gctgttcagg
     1321 atgacgatga gcttttttgc atcgatgcct tcggctatga cggcacaggc tacattagca
     1381 ctagcggcat ctgctcttac gttgatggcg cggtgtcggc tggcaacatc cctgcacgct
     1441 ggggctggtg gaccattcct ggcgtgacgc agcccaatat gatcctcgat tcagtcggca
     1501 aacttaccat ctgggggatc aacgcagata ccgtcaagca attgatgagt ctccaaacca
     1561 ccggcgaagg cgcgctgaat atatcaaatg ccagcgactt cgattatttc aatcccataa
     1621 tttttactaa tcgggcccgt ggaactatcg gtgcgcgaac cgccgtggcc tccgccgatt
     1681 cagcgtttga aatcttggta catggctaca acggtaccac ggacttaccc cttgcctaca
     1741 tacaggcgct cgttgacggc acgcccggta caaatgacat gccgggcaga attgaattcc
     1801 acacacaagc tgacggtgca gggggaagtc tcgaaggcac aacgcctgaa atggtcatca
     1861 agaacaatgg caatgtcggt atcgggactg cgacgccaac aggcttgctt gaagtcgctt
     1921 ccagcaccgt cgaagatctg ctgatcctcg atgttgcaaa tgacatcgcc ggtactgatc
     1981 cttcgatttt gcttcggcgt tcgcggggaa ccctttccgc ccggacgacc gtaagctcgg
     2041 gcgacaatct cggggccatc tgggccagcg gctatgatgg aagcaatatg gagtatgctg
     2101 cgtcaattga attcgaagtc gatggcggag ttgccaccgc aggcgataca accgatatgc
     2161 cgggggctat caggtttaaa acaacaccgg atggatccaa cgtctcggtt gaacgcatga
     2221 gaatcgacga tgcgggcaat gtcggtatcg gcaccacgtc gcccgcatca aagttcgtcg
     2281 tcgcaccgcc tgcttcgcaa gcgattgccg gggcggacac gattactgct gacgcctgcg
     2341 gcacgatcaa gcagatcacc tccgccggaa acgtgacaac gaacaccacg aacacgttga
     2401 ccgcgcccac ggcctcctat gccgggtgct gcatgaccgt catcaatgtg gacacggcgg
     2461 atacgatcac cctcgacgcc aacacgcttt tcaaaaccat cggcggggca aatcaggccc
     2521 ttggcccctc cgacaccgtg caggtctgct cgaacggcac caactggtac caggttgggg
     2581 ccgtgtcagg gaaccaatga tgcggtgatt atgcaacttg tactgaggga cctgcggttc
     2641 cctcattggg ttcgcaacgg tacgttatcc tcgttaacca ttcattaagc attgggttgg
     2701 tagctcgcct tcgaagaaaa ttgaacccaa cctgcccgca aaccgcagaa atccgccatt
     2761 cttaactgtt ccttaaatca ttagttgtat tctggaaacg ggtacagcgt gcgggtgtat
     2821 gcgtatcgtt tcgatgaact gcaataacag gcctttctcc cctgtttccc ggcagaacgc
     2881 tgcaaccctg ataaaagaca gcatcgatgg ctttctcaga tcagttcact agccaatatt
     2941 catgagcgac aatggcgcta gatattgaga aatatagaca ttacgttgcg gggtttgact
     3001 tatcacccga ggaacaggcg gagctgatcc gcactgtgtg gatggtgtta gagagtttcg
     3061 tcgatcaagc ttatggacgg catccccacc agcactgcct ccgcaacctg gaatttaagg
     3121 a
//
LOCUS       DAORQN010000173         3116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1024_length_3116_cov_4.491343, whole genome shotgun sequence.
ACCESSION   DAORQN010000173 DAORQN010000000
VERSION     DAORQN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1024_length_3116_cov_4.491343"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PK614_08115"
     CDS             <1..384
                     /locus_tag="PK614_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HRB82213.1"
                     /translation="GLIEMGEKKQSRSFFNVGMAKKFMQTMLVADALAELQQADLTTS
                     LREIYYRTKHTIQDSHENTFDTQDESDPIIEDLEVSLVALREELHVRAENSGSIVGPV
                     VFGDDGDRVDCAKLGKGGYSVPSIG"
     gene            421..954
                     /locus_tag="PK614_08120"
     CDS             421..954
                     /locus_tag="PK614_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82214.1"
                     /translation="MLLLVENGTQCNRLSEDKFWRRYNCVLLTGNGQPPRGVRRLARR
                     LHEEHKLPVYVLVDNDPWGYYIYSVVKQGSINLAFERQRMAIPKAKFIGLSSADPERY
                     ELPRNVGIKLNEKDITRAKELLNYQWFQKPAWQQEIKRMLANGLKYELDALANKDFQY
                     LTKKYLPRKLKEKDWLD"
     gene            1027..1332
                     /locus_tag="PK614_08125"
     CDS             1027..1332
                     /locus_tag="PK614_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82215.1"
                     /translation="MNMLMLAFRSSLKERVHVLLEQCDVRAYTEMPETTGAGQTGPAD
                     GVSFYPGVNNVIMVAVDDAHAERVERAVQAWRDEVLQRPGGQKPSITVFSWPCRQLV"
     assembly_gap    1723..1732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1924..2397)
                     /locus_tag="PK614_08130"
     CDS             complement(1924..2397)
                     /locus_tag="PK614_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82216.1"
                     /translation="MNFRLVLTVVALGFIVLLLVIGIRTAVRWLKIHYPQRANAVLFG
                     VCLLLAGAGVLIAIEMKDQPTFRPNDLLTLQEPVVARTIVRDRESRSIACVVDLHEHL
                     GVLELDNERGSLRALVESNNTSVPLYCPIGAEVRIEAAWLHHPTITHRQAATDGP"
     gene            complement(2394..>3116)
                     /locus_tag="PK614_08135"
     CDS             complement(2394..>3116)
                     /locus_tag="PK614_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB82217.1"
                     /translation="ALLDLIPAMVWYKDAHNRILRANRRAAASIKKTVAEIEGRSTDE
                     FYPEEAERYHQDDLEVIVSGRPKLGIIEPYQTGDGEKCWVQTDKVPYLDPQGNVLGVL
                     VFAQDITERKRTEEALRESADCLRLVMESASNGIIVVDAEGMILLTNLHVDTQFGYER
                     GELPGQPAERLMPARLRPQGSDRAGVFLIRPDDRSVSPVCECVGLRKDGTEFPFTMTL
                     TPLMTTNGLYSVVAVNQAQGIA"
BASE COUNT          678 a          905 c          906 g          617 t
ORIGIN      
        1 gggctgatcg agatgggcga gaagaagcaa tcccgctcgt tttttaacgt cgggatggcg
       61 aagaaattca tgcagacgat gctggtggcc gatgcgctgg cggagttgca acaagccgat
      121 ctgaccacct cgctcaggga aatctattat cgtacgaaac atacgattca ggactcccat
      181 gagaatacct tcgatacaca ggacgagtcc gatcccatta tcgaggatct cgaagtctcg
      241 ctggtcgcgc tccgggaaga actccacgtg cgcgcggaaa acagcggcag catcgtcgga
      301 ccggtggtgt tcggggacga cggtgaccgg gtggattgtg ccaagctggg caagggcgga
      361 tattctgtgc cctcgatcgg ctaacctgaa tatctgtaga tccgccgctg taccgccgat
      421 ttgctcctgc tggttgaaaa cggcacgcag tgtaaccggc tctcggaaga taagttctgg
      481 cggcgataca actgtgtgct tctgacgggg aacggtcagc caccgcgcgg tgtgcggcgg
      541 ctggcgcgcc gcctccatga ggagcacaag ctccccgtgt atgtgctggt cgacaacgat
      601 ccctgggggt attatattta ttcggttgtg aaacagggct cgatcaacct cgcgtttgag
      661 cgccagcgca tggcgattcc caaagcgaag ttcatcggac tctccagcgc agatccggag
      721 cggtatgagt tgccgcgtaa tgtgggcatc aagctgaacg agaaagacat tacccgcgcg
      781 aaggagctgc tgaactatca gtggttccag aagccggcct ggcagcagga aatcaaacgg
      841 atgctcgcga acgggctgaa gtacgaactg gacgcgctcg cgaacaaaga ctttcaatac
      901 ctgaccaaga agtatctgcc gcgcaagctg aaggaaaagg actggttgga ttagcggtgg
      961 aactccgcat tgcctccaac cgggcctcac gtcgaacgag cctcgttacg ttcctggagt
     1021 cacgatatga atatgctgat gttggccttc cggtcgtcat tgaaggaacg ggtgcatgtg
     1081 ttactcgaac agtgcgatgt tcgcgcctac accgaaatgc ccgagacgac cggagccgga
     1141 cagacgggac ccgctgacgg tgtctccttc tatccggggg tcaacaatgt gatcatggtc
     1201 gccgtggacg atgcgcatgc cgagcgggtt gaacgagccg ttcaggcgtg gcgtgatgag
     1261 gtgctccaac gtcccggtgg acagaagccc tccattacgg tatttagctg gccttgcagg
     1321 cagctggtgt agccgaagaa acggagtctc tgtgagtcgg catggcagat cgtatccggg
     1381 gcgggttcgc gtggatatct gtgccctcgg cctcagtgtg gccatgatgc tggccccgtc
     1441 gttcgtgacg gcccagactt caccaccggc atcgacccca cagaatcagc ccggacaaaa
     1501 cccgcaacca ctgggacggc tcgcttagat tcacttgacg aatacgtacg ctcttcgtcg
     1561 tttacaggga acgaccagga gagtcaagcc ttgagcgtat tctcgtcggc atctcgatgc
     1621 aggaaaacac ggtgcaacgg tggacgaaat gcggggtgat cgaaccggga gtgatgagga
     1681 gcgacgatgc agggtggtga cggaggtttg ggtgggagga ggnnnnnnnn nncacctacc
     1741 gaggagaggt agatacggcg gagatctcgg gaggatttca ggcttcccag tacaagctgg
     1801 gcatgcacac caccctcaac actcaatccc ctttttgtac atattccccc ggggcgttaa
     1861 ctccccccac ccggttacga taggcctcct cacgtcgatg agtcaagagc atgggcaggc
     1921 gatttacgga ccgtccgtgg cggcctgtcg atgtgtgatg gtcgggtgat ggagccaggc
     1981 cgcctcgatc cggacctccg ccccgatcgg acagtacaac ggcactgatg tattgttact
     2041 ctcgaccagc gctctcaagg aaccgcgttc gttgtcgagc tccaacacac ccagatgctc
     2101 atggagatcc accacgcagg cgatgctccg ggactcgcga tccctcacga tcgtccttgc
     2161 caccaccggc tcttgcagcg tgagcaggtc attcggccgg aaggtcggtt gatccttcat
     2221 ctcgatggca atcaacacgc ctgctccggc cagaagcagg cacaccccga acaaaacagc
     2281 attggcccgc tgcggataat ggatcttcaa ccaacgaaca gccgtgcgga tgccaatgac
     2341 caacagcaga acgatgaaac ccagcgcgac gacggtcaac acaaggcgaa agttcatgcg
     2401 atcccttgtg cctggttgac ggcaaccact gaatacagtc cgttcgtcgt catcaacgga
     2461 gtcagcgtca tcgtgaatgg aaactccgtg ccgtctttcc gtaagccgac gcattcacaa
     2521 acagggctca ccgatcggtc gtccgggcgg atcaaaaaaa cgccagcgcg gtctgagcct
     2581 tgcgggcgca gtctcgccgg catgagccgt tcagcaggct gaccgggcag ttcgcctcgc
     2641 tcatagccga actgcgtgtc cacatggaga ttcgtcagca gaatcatccc ttcggcatcc
     2701 accacgatga tgccattcga ggccgattcc atcaccaggc gaaggcaatc cgcgctctca
     2761 cgcaacgcct cttcggtccg tttccgttca gtgatgtcct gcgcgaacac caagaccccg
     2821 agcacattcc cctgcgggtc caggtacggc accttgtcgg tttgcaccca gcatttttca
     2881 ccgtcccctg tctgataggg ctcaatgatc ccgagcttcg gccgccctga aacgatcacc
     2941 tccagatcat cctgatgata ccgctcggcc tcttccggat aaaattcatc ggttgaccgg
     3001 ccctcgattt ccgccacggt tttcttgatg gatgccgccg cacggcgatt cgcgcgcagg
     3061 atccggttat gcgcgtcctt gtaccagacc atggccggga tcaaatccaa cagcgc
//
LOCUS       DAORQN010000174         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1028_length_3092_cov_22.554495, whole genome shotgun sequence.
ACCESSION   DAORQN010000174 DAORQN010000000
VERSION     DAORQN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1028_length_3092_cov_22.554495"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK614_08140"
     CDS             <1..231
                     /locus_tag="PK614_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82218.1"
                     /translation="KGGNTRRVPFSQTLKDGAAPATIEVVLSYALIPEPEAELKAKYL
                     ATLANDQERATAKKVIEEYIQPRMLTYRAKSL"
     gene            300..1538
                     /locus_tag="PK614_08145"
     CDS             300..1538
                     /locus_tag="PK614_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRB82219.1"
                     /translation="MKNRRGLLQGFVIGLVLLAVAITSYGVQQGLAQDAKAQATIEKA
                     FPSSSKCKRCHERVFEEWETSPLSRSIHTPTFRAALDAYLTSSAGKDKALCFRCHAPH
                     VREFADQAQLFVTQAQSGEPSLDGVACVQCHLIKQVDRTKQPPEPKYDLGSKTMYGPY
                     KDFAQNLAHQSMELGLFHKSDLCLNCHQVVPAAADLGKSNDLLGNWDQSKAVKSGKEC
                     QTCHMPEQVGESANGEAKRKVANHTFPGRIGQLRQEAAKLEVSTKVEGEKTTVTVAVQ
                     SLVPHNLPTTHPGWASVVLELDIKGKNLKTVFNDKRVYGRTYADAKGQKTVFDFEAIK
                     VLEETVLKPEEKRLETFTFTTPKDTKTFDVEAILSYAPVTGPSAFLQRIEAESSKGAQ
                     DPVFQSIPIAKFSENIPVAR"
     gene            complement(1627..1881)
                     /locus_tag="PK614_08150"
     CDS             complement(1627..1881)
                     /locus_tag="PK614_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82220.1"
                     /translation="MGLAPEYILIDQQKFSKTKLALDNHVYKNCEIDDCDVYYSGGQY
                     ELIDTHITNSRLILNHPAKGIYSAIQIFKMKSPGSSIVSE"
     gene            2264..>3092
                     /locus_tag="PK614_08155"
     CDS             2264..>3092
                     /locus_tag="PK614_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRB82221.1"
                     /translation="MTFALIAAIAGIAYGLYLAMWVFKLNAGNAKMQEIAKAIQEGAG
                     AYMNRQYKTVGMVAAVLFVVLWGAGAVSDKFGMLTAIGFLIGAGASALAGYVGMIIAV
                     RANVRTAQAAHNGMNAALTVAFRGGAVTGLLLIGLGLLAITSFYTLASQMAGQEKAIH
                     ALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMAADLFETYAVTTVAAMVLAFTMFKGVSAPILYPLDSRFHIREQKWIVQPA
                     "
BASE COUNT          650 a          843 c          942 g          657 t
ORIGIN      
        1 aagggcggca atacgcgccg tgtcccgttc tctcaaacgc tgaaggatgg cgccgcgccg
       61 gcgacgattg aggtcgtgct cagttatgca ttgatcccgg aaccggaagc ggagctcaag
      121 gcgaagtatc tggcgactct ggcgaacgat caggagcggg cgaccgctaa aaaggtcatt
      181 gaggaataca ttcagcctcg tatgctgacc tatcgggcca aatcactcta agctcccctg
      241 cagcgggccg tgcaccacag gcagcgagga ttgagtactg tgcgaaggaa acgccgacgg
      301 tgaagaatcg acgaggattg ctgcagggat tcgtgatcgg cctggtgttg ctggcggtgg
      361 ccatcaccag ctacggcgtg cagcagggat tagcccaaga tgccaaggcc caggccacga
      421 tcgaaaaggc ttttcccagc tcaagcaagt gtaagcgatg tcatgagcgt gtgtttgaag
      481 agtgggagac gtcgccgctt tcgcgttcga tacatacgcc gaccttccgg gcggcgctcg
      541 atgcctacct gacctcctct gccgggaaag acaaagccct gtgctttcgc tgtcatgctc
      601 cgcatgtgcg cgagtttgcc gatcaggctc aattgttcgt gactcaagcc caatccggtg
      661 agccgtcatt ggatggtgtg gcctgcgtgc aatgtcacct catcaagcag gtcgatcgga
      721 cgaaacaacc gcctgagccg aagtacgatc tcggcagcaa gaccatgtat ggcccgtata
      781 aagatttcgc gcagaacctt gcgcatcaat ccatggaatt ggggctcttc cacaagtctg
      841 acctgtgctt gaattgccac caggtggtgc ccgctgcggc ggacctcggc aagagtaacg
      901 acttgttggg gaattgggac cagagcaaag ccgtgaagtc gggcaaggaa tgccagacct
      961 gtcacatgcc tgaacaggta ggggaatccg ccaatggaga agccaagcgg aaggtggcga
     1021 atcatacgtt cccgggccgc atcggtcagc tcaggcagga agcggccaag ctggaagtct
     1081 cgaccaaagt agaaggggag aaaactaccg tgacggtagc ggtgcagagc ctggtcccgc
     1141 acaacctgcc gaccacccac cccggttggg cgagtgtcgt cttggaattg gatatcaagg
     1201 gcaagaacct gaagaccgtg ttcaacgaca aacgcgtcta cggacggacg tacgccgacg
     1261 ccaaaggcca gaagacggtg ttcgattttg aggccatcaa ggtgcttgag gaaacggtac
     1321 tcaagccgga agaaaagcga ctggaaacgt tcaccttcac gacaccgaag gacaccaaga
     1381 ccttcgacgt cgaggccatc ctcagttacg ctcctgtgac cgggccatct gcctttttgc
     1441 agcgcattga ggcggaatcc tccaaggggg cgcaggatcc tgtgtttcaa tccatcccca
     1501 ttgcgaagtt cagcgagaac attccggtcg ccaggtaggg atacccgttc ttcgtcacgc
     1561 gtgcctcgac gtggccaaat ttcccacggt cacacgagag atgaacgacg cgtcacgaag
     1621 tgcggattac tcggagacga tgctggaacc gggggacttc atcttgaaga tctgaatggc
     1681 gctgtagatg cccttggccg ggtggttgag gatgagtcgt gagttggtga tgtgcgtatc
     1741 gatcaattcg tactgtccgc cgctgtagta cacatcgcag tcgtcgatct cgcaattctt
     1801 atacacgtga ttgtccagcg ccagcttggt tttactgaac ttttgctgat cgatcaggat
     1861 atattccggc gccagtccca tcgagtactc ctcaatccgc gcgcactata acaaagtcgc
     1921 tgtccttccg caaacggtga gccgcgaggc ttcgggcgag gtgtcgtgcc tccaaccctt
     1981 cacgccggcc atctttcgcc ttgatccgat tccgacctcg ctatagaatg gcgccacttt
     2041 cccgctcgtt cgcaccgtgt ctgttgtcgg gcatgcagta tttcttcctg ctgcattctc
     2101 agctcgcgat gggtttagtc cacggagatt ctgtgacatt cgtcgaccgt tgtacgtgac
     2161 tggttcatgt tcgcgacggg gtattctcgt agacacttga gctggattct cagtttcacc
     2221 attctgttaa caggaggaga acactgtgag cgactcagca attgtgacct ttgctctgat
     2281 tgcggcgatt gccggtattg cctatggcct ctatcttgcc atgtgggtgt tcaagctcaa
     2341 cgcgggtaat gcgaagatgc aggaaattgc gaaagcgatt caggagggtg ccggcgccta
     2401 tatgaatcgc cagtacaaga cggtgggcat ggtggcggcg gtgctgttcg tagtgctctg
     2461 gggggcgggc gccgtatcgg ataagttcgg aatgctgacg gccatcggat ttttaatcgg
     2521 cgccggggcc tcggcactcg ctggttatgt gggtatgatc atcgcggtcc gggcgaacgt
     2581 gcggacggcg caagccgcac acaacggcat gaatgcggcg ttgaccgtcg cgttccgcgg
     2641 cggcgcggtg acggggctgt tgctgatcgg gctcggcctc ttagcgatca ccagtttcta
     2701 cacactggcc tcccagatgg ctgggcagga aaaggcgatt catgccttgc tcagccttgg
     2761 cttcggcggc agtttgatct cggtgtttgc gcgcgtcggc ggtggcatct acaccaaagc
     2821 ggccgacgtg ggcgcggacc tggtcggcaa agtggaagca ggtattcctg aagatgatcc
     2881 tcgcaatccg gcggtcatcg ccgacaacgt gggcgacaac gtcggcgatt gcgcaggcat
     2941 ggcggcggac ctttttgaaa cctacgcggt gacgaccgtg gccgcgatgg ttctcgcgtt
     3001 cacaatgttc aagggcgtca gcgcgccgat tctgtatccg ctcgacagcc ggttccatat
     3061 cagggagcaa aaatggatcg tgcagcccgc cc
//
LOCUS       DAORQN010000175         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1029_length_3092_cov_5.499177, whole genome shotgun sequence.
ACCESSION   DAORQN010000175 DAORQN010000000
VERSION     DAORQN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_3092_cov_5.499177"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK614_08160"
     CDS             <1..816
                     /locus_tag="PK614_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRB82222.1"
                     /translation="SFAYHYIWRHRVTYSWAVLHDHLCLFADCPDGLFMALPPLGPGP
                     LAQPLAEAFRFMRERNGDSRVTRVENVPEACVDEVRALGYRATAKEPDYLYDASALSD
                     LSGESFKSPRAACNRFVREWGGMLEPYEPRDERACVSLFHEWREQKQQAGSDDWACAL
                     LEDAAGAHETALSDAAELGLTGAVVRVGGRIRAYTMGLWLNPSVFCVLLEVADRDIVG
                     LGPFVFREFCRQARAKGACWINTMDDSGLPSLARSKQWYHPARLLPNYIVTES"
     gene            819..>1003
                     /locus_tag="PK614_08165"
     CDS             819..>1003
                     /locus_tag="PK614_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82223.1"
                     /translation="MAASLPPGSPRRSYPWLPVLIVGVTIVALVIGVVMLRSIEVRMV
                     EATGENLTLASAEIADKL"
     assembly_gap    1004..1036
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1045..>3092
                     /locus_tag="PK614_08170"
     CDS             1045..>3092
                     /locus_tag="PK614_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB82224.1"
                     /translation="MMARAFSDRALDKKYINEYLQWMKETYAPVYLWLGVTDAHGRMV
                     AATDSSVIGQDYSRSGWFDRVRQTGTVQVDEVEIHESNHKIESVAFTAPILSVGGQFL
                     GAVTTRIGLPMLEDVLIETVREIQQTDGSAGPFEYQFLTKAGVVFVDSASSGIDRVNL
                     TELGLPSVLLSRSGKAGYVEEMHVRRHVPVVTGYSQTKGYSDYSGLQWTILLRMDRDL
                     ILLPIRAMMWKLGMAGVVVWLPMVGLLLWATGRLREQHRQAQQESEWARAAEAALLQS
                     QERNRVIVDTALDAVISMDAGGIITDWNAQAVNIFGWEREEALGRRVSETVIPVRDRD
                     AHERGLRHFLDTGQGALLNRRIEMMGSHRDGHEFPVEITISPARLGDAYIFSAFIRDI
                     TARKRTERQLASQYAVTRVLAESRTLEEAGPKILQAICESLEWELGIFWRLDRQGSVL
                     RCLDVWQSPALNAEEFVLDTWQHTFTIGKGLPGRIWQAGEPTWIKDVVQEANFPRGGA
                     AARVGLHGAFGFPVRVGTEVEGVIELFRREIKQPDEQLLKMVADVGLKIGQFGERARA
                     EDALRRTEAQLQQSQKMEAVGRLAGGVAHDFNNMLTVIRGYSELVLSRLAPTDGFRKE
                     LEEVKKAADRASGLTGQLLAFSRRQFIAPKVLDLNSVIHNMEGMLRRLLGEDIVELCT
                     V"
BASE COUNT          553 a          831 c         1016 g          659 t
ORIGIN      
        1 tccttcgcct accattacat ctggcgacat cgcgtgacct attcctgggc cgttctccac
       61 gaccatctct gtttgttcgc cgactgtccc gatggcctgt tcatggccct gcccccgctt
      121 gggcctggtc ctctcgcgca gccgttggcc gaggcctttc gtttcatgcg tgagcggaac
      181 ggcgattccc gggtcacgcg ggtcgagaat gtgccggagg cctgtgtcga tgaggttcgc
      241 gcactgggct accgtgcgac tgcgaaagag cccgactatc tctacgacgc gtcagctctg
      301 tccgacttgt ccggcgagtc gttcaaatcc ccgcgggccg cgtgcaatcg atttgtccgg
      361 gaatggggcg ggatgcttga gccctatgag ccgcgggacg aaagagcctg tgtcagcttg
      421 tttcatgaat ggcgggaaca gaagcagcag gccggttctg atgactgggc ctgtgcgctg
      481 ttggaggatg ccgccggcgc gcatgagacg gcattgtctg acgctgccga actcgggctg
      541 accggcgcag tagttcgggt tggaggccgt attcgggcct atacgatggg cctgtggctc
      601 aacccgtcgg tgttttgtgt gttgctggaa gtcgccgatc gggatattgt ggggctcggg
      661 ccgtttgtgt ttcgtgaatt ctgtcgccaa gctcgcgcga aaggggcatg ttggatcaac
      721 acgatggatg attccggcct gccgagtctg gcccgttcca agcagtggta tcatcccgcc
      781 cgattgttac caaactacat tgtgacggag tcctgagtat ggccgcctcg cttccacccg
      841 gttccccgcg tcggtcctat ccctggttgc cggtcctgat cgtcggcgtg accattgtgg
      901 cgcttgtgat cggcgtggtg atgctccgct ccattgaagt gcgaatggtc gaggcgaccg
      961 gagaaaatct gacgctggcc tccgctgaaa tcgcggacaa actnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnatgt tcgcatgatg gctcgagcct tttccgatcg cgccctcgat
     1081 aaaaaataca tcaacgaata tctgcagtgg atgaaggaaa cctatgcgcc ggtctatctc
     1141 tggctgggcg tgacggacgc ccatgggcgt atggtggcgg cgacggactc ctccgtgatc
     1201 ggccaggact atagtcgaag cggctggttc gaccgggtgc ggcagaccgg aaccgtgcag
     1261 gtggatgaag tcgagatcca tgaatcgaat cataagatcg agtccgtggc ctttacggcc
     1321 cccattctga gtgtcggcgg ccagtttctc ggtgccgtga cgacccgcat cgggctgccg
     1381 atgctggagg atgtcctcat tgaaacggtc cgtgaaattc aacaaaccga tggctccgcc
     1441 ggccccttcg agtatcaatt cctgacgaaa gccggtgtcg tgtttgtgga ctcagcttcc
     1501 tccggtatcg atcgggtgaa tctcacagaa ctcgggttgc catcggtgct gttgagccgg
     1561 tccggcaagg cgggctacgt cgaagagatg catgttcgcc gtcacgtccc ggtcgtcacg
     1621 ggttattctc aaaccaaggg gtatagcgac tattcgggat tgcagtggac gatcctgctg
     1681 cggatggatc gtgacctcat tctcctgccg attcgagcca tgatgtggaa actgggcatg
     1741 gccggtgtcg tcgtctggct cccgatggtc ggcctgttgc tctgggccac cggtcgattg
     1801 cgcgaacagc atcgtcaggc gcagcaggaa agtgaatggg cgcgtgcggc ggaggccgcg
     1861 ttgctgcaga gtcaggaacg caaccgggtc atcgtcgata cggcgctgga cgccgtcatt
     1921 tctatggatg cgggcggcat cattacggat tggaatgcgc aggcggtcaa tattttcggg
     1981 tgggagcggg aggaagcgct cggccgccgc gtgtccgaga cggtgattcc tgtgcgcgat
     2041 cgggacgcgc atgagcgcgg attgcgccat ttcctggata ccgggcaggg cgcactcctc
     2101 aatcgacgga tcgagatgat gggatctcac cgcgacggtc atgagtttcc cgtcgaaatc
     2161 accatttctc ctgcccggct gggcgatgcc tatatcttca gcgcgtttat ccgggatatt
     2221 acggctcgga aacgtaccga gcgccaactc gcatctcagt acgcggtcac gcgcgtcttg
     2281 gcggaatcgc ggacgctgga agaagcgggg ccgaagatcc tgcaggccat ctgtgaaagt
     2341 ctcgaatggg agttgggaat attctggcgg ctcgatcggc agggaagtgt gctgcgttgt
     2401 ctggatgtct ggcagtcgcc cgcattgaac gccgaagagt ttgtgttgga cacctggcaa
     2461 cataccttta cgatcggcaa gggactcccc ggacggattt ggcaggccgg ggaaccgacg
     2521 tggatcaaag acgtggtgca ggaggccaat ttccctcgcg gtggcgccgc agccagagtg
     2581 gggttgcacg gggcgtttgg atttcccgtt cgggtcggca ccgaagtcga gggtgtgatc
     2641 gagttgttcc ggcgcgagat caagcagccg gatgagcagc tgttgaagat ggtggccgat
     2701 gtcggactga aaatcggcca gttcggtgaa cgcgcgcggg ccgaggatgc gttgcggcgc
     2761 acggaagcgc agctccagca gtcgcaaaaa atggaagccg tcggacggct ggccggcggc
     2821 gtggcgcacg acttcaacaa catgctgacg gtgattcgtg gatacagcga actggtgttg
     2881 agccgcctgg cgcccacgga tggattccgg aaggaactgg aggaagtgaa gaaggcggcg
     2941 gatcgcgcct ccggtctcac cggacagctg ttggcgttca gtcgccgcca attcattgcg
     3001 ccgaaagtgc tggatctgaa ttcggtgatc cacaatatgg aaggcatgtt gcgacggttg
     3061 ctcggcgagg acatcgtcga gctctgcacg gt
//
LOCUS       DAORQN010000176         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1040_length_3072_cov_4.889957, whole genome shotgun sequence.
ACCESSION   DAORQN010000176 DAORQN010000000
VERSION     DAORQN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1040_length_3072_cov_4.889957"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK614_08175"
     CDS             <1..673
                     /locus_tag="PK614_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRB82225.1"
                     /translation="QVMSYHRIPYPEFMVVPLHRMVRRPKYLPFPLIVKSISEEASLG
                     ISQASIVDDDEKLRERVAFIHDNVGTGALIERYIEGRELYVGVMGNAHLQVFPVWELV
                     MDKMPDEARRIATERVKWSRKYQDKYGICSCEARNLPDGVVDHIQHLAKRVYRALGLS
                     GYARIDMRMDKDGNVYVLEANPNPQIATGEDFADSAEKADLAYKDLLQELLHVGLRWR
                     PAQAA"
     gene            complement(1021..1572)
                     /locus_tag="PK614_08180"
     CDS             complement(1021..1572)
                     /locus_tag="PK614_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82226.1"
                     /translation="MTPMRPVRALFIGLAFLGILWAAPTTAAEVQEPRWGFSTDLGLW
                     SGTTNDTTFALGFGLDYYMDQNFSFGGMALFTPVGDLTQIGIAGVAKYHLRLNSGFNL
                     VPFAGLGILHADLNRGSGPTKVDRNDTSHFIPLGMSLEYQVGPKIAFSTTLMVNLHHI
                     TLSPPVPNDNTSVALLFGIRWGP"
     gene            1719..2213
                     /locus_tag="PK614_08185"
     CDS             1719..2213
                     /locus_tag="PK614_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82227.1"
                     /translation="MPKGAMTPAEVADALYKLIPRRVSVELLSEYGIEGQEEHEETMT
                     RELLSFTLYWVHAAVNAHIPRKYREVLFQRVLELIQADWVATFKLESVKWEDYLVEME
                     ERRALYAPVGDYEGGAMAASEEISDLLENQCLIQPEDRPKLLVLLPDLVPLDKYQELL
                     SQCV"
     gene            complement(2235..2858)
                     /locus_tag="PK614_08190"
     CDS             complement(2235..2858)
                     /locus_tag="PK614_08190"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRB82228.1"
                     /translation="MSKLVLIRHGESQWNLENRFTGWVDVPLSPKGIEEAKAAGKKLA
                     GFKFDRAFSSVLARANETLRLVLEGIGQTGIPIEKDKALNERMYGELQGLNKAETAKK
                     FGDEQVKIWRRSYDVRPPGGESLKDTAERVSSSTCSLFNCSSRIRPYVLQGETILIAA
                     HGNSLRALVMQLEQLTREQVLELNIPTGAPLLYELDNSGKVLSHRYL"
BASE COUNT          603 a          868 c          928 g          673 t
ORIGIN      
        1 gcaggtgatg tcgtaccatc gtattccata ccctgaattc atggtcgtgc ccctgcatcg
       61 catggtgcgg cgacccaaat acctgccctt cccattgatc gtgaagtcga tcagcgaaga
      121 ggcatcgctg gggatttcgc aggcctcgat tgtcgacgac gatgagaagt tgcgggaacg
      181 ggtggcattc atccacgaca atgtcggcac tggagccctc atcgagcggt acatcgaagg
      241 acgcgagctc tatgtcgggg tgatgggcaa tgcgcacctg caagtgtttc cggtctggga
      301 actcgtgatg gacaagatgc cggatgaggc ccgccgcatc gcgacggagc gagtgaagtg
      361 gagccgcaag taccaggaca agtatggcat ctgctcgtgc gaagcgagga acctcccgga
      421 cggagtcgtc gaccatattc agcacctggc caagcgcgtc tatcgtgcat tggggctgag
      481 tggttacgct cgcatcgata tgcggatgga caaagacggt aacgtctacg tgctcgaagc
      541 gaatcccaat cctcagatcg ccaccggcga agattttgcc gactcggccg aaaaagccga
      601 cctcgcctac aaagacctgc tgcaggaatt gttgcatgtg gggttgcggt ggcgccccgc
      661 ccaagccgcc tagcggatgt tgaaacgggc ttacggctgc gttctcgctt cgctcagacc
      721 ctcaacgtac tgtgctcaag tacgactcgg gccttcactt gctgcggcct tgccgcaaga
      781 cccgtttgaa catccgcggg attgctgaaa cggttcccca tcttcgttct cgtctcgctc
      841 cggggctcaa cgtaccgcaa gggtacgcct cgcctcctcg ccttgctgcg gcctcgctgg
      901 aaacccgttt gagcggctgt ggaatctgaa accgaagggc gtgcggctgc gttctcgcgg
      961 tactcaaatt ttcgacatgc tcagtgcagt tcgccggagc tgtcgttcac tgtggcctcg
     1021 ttacggcccc cagcggatgc cgaagagcaa cgcgacgctg gtgttgtcgt tcgggacggg
     1081 tggcgagagg gtgatgtggt gcaggtttac catgagggtg gtcgagaagg cgatttttgg
     1141 accgacctga tattccagcg acatgcccag gggaatgaaa tggctcgtgt cgttgcgatc
     1201 gactttggtc gggccgctgc ctcgattgag gtccgcgtgg agaatgccta gtccggcgaa
     1261 cggcaccaga ttgaatccgc tattcaggcg aagatgatac ttcgcgactc ccgcgatgcc
     1321 gatctgtgtc aggtccccga ccggcgtgaa gagcgccatc ccgccgaacg aaaaattctg
     1381 gtccatgtag taatcgagcc cgaagccgag tgcgaaggtg gtgtcgttgg tggttccgct
     1441 ccagaggcca agatcggtgc tgaagcccca gcgtggctcc tgcacctctg cggctgtggt
     1501 cggtgcggcc cagaggatgc cgaggaaggc gaggccgatg aacagcgctc gaacaggtcg
     1561 cataggtgtc atggtgctcc atctcacggt gatgaggctc tatgtagcat aggcctttcc
     1621 ggcggacaag caatcgacgc cgcgtggcag ttcaccgtcc agggttgtca atgcgcgcgg
     1681 ggctccagta tgctgagacc cctttcggga ggatgagtat gccaaaagga gccatgacgc
     1741 cggccgaagt tgctgacgcc ttgtacaaac tgattccacg ccgggtttcc gttgagctgt
     1801 tgagcgaata tggcatcgag ggccaggaag aacatgagga gacgatgaca cgagagctcc
     1861 tctctttcac gctctactgg gttcatgcgg ccgtcaatgc ccacattcca cggaaatacc
     1921 gcgaggtcct gtttcagcgg gtgctggaat tgattcaagc ggattgggtt gcgacgttca
     1981 agcttgagtc ggtgaagtgg gaggactacc tggtcgaaat ggaagaacgg cgcgcactct
     2041 atgcgccggt gggggattac gaaggcggcg ccatggccgc ctccgaggaa atctcagatc
     2101 tgctcgaaaa tcaatgtttg attcaaccgg aggatcggcc gaagttgctc gtgctcctgc
     2161 ccgatctggt gcccttggat aagtaccagg aattattgag tcagtgtgtg taggctgtcg
     2221 gtcgtgtcct ctggttagag ataccggtgt gacagcacct tcccgctgtt gtcgagctca
     2281 tagagcagcg gggctccggt cggaatgttg agttccagca cctgctctct ggtcaattgt
     2341 tcgagttgca tcaccagcgc ccgcaggcta ttcccatggg cagcgatgag aatggtttcc
     2401 ccctggagca cgtagggcct gatgcgactc gaacaattga acagagagca ggtgctggaa
     2461 ctcacccgct ccgctgtgtc tttcaaactt tctccgcccg gtggccgtac atcgtaactc
     2521 cgccgccaga ttttcacctg ctcatctccg aacttcttgg cggtctcggc cttattcagc
     2581 ccctgaagct ctccgtacat gcgctcgttt agcgccttgt ctttttcaat gggaatgccg
     2641 gtctgtccga tcccttcgag gacgaggcgc aacgtttcgt tggcgcgggc cagcacggac
     2701 gaaaaggcgc gatcgaattt gaaccccgcc agtttttttc ctgccgcctt cgcctcttcg
     2761 atgcctttcg gggagagggg gacatccacc cagccggtga agcggttttc cagattccac
     2821 tgcgactcgc catgacgaat gagaaccaat ttgctcatcg tacctcgtct ccttgtccga
     2881 aaggatagaa cggtttcagg acagcttatg cgatttcccc ttcgccctgt caagcgggat
     2941 gagcccacgg aagtgcgccg tggctccgtc atgctgagtt gcaatcagat gaggcagcca
     3001 gtaccatgcc gccggtttct cctcacgccg gagcacatcg atgaccgaac gttccccctc
     3061 agactggact cc
//
LOCUS       DAORQN010000177         3065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1044_length_3065_cov_20.361130, whole genome shotgun sequence.
ACCESSION   DAORQN010000177 DAORQN010000000
VERSION     DAORQN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_3065_cov_20.361130"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /gene="dnaX"
                     /locus_tag="PK614_08195"
     CDS             <1..1575
                     /gene="dnaX"
                     /locus_tag="PK614_08195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRB82229.1"
                     /translation="DDVREIRENVKFTPFRGQYRVYIIDEVHMLSNSAFNALLKTLEE
                     PPAHVVFIFATTEIHKIPATILSRCQHYNFRRIAKAEIVQRLRHVADQDGLTIEDRSL
                     MALARASEGSMRDGLSLLDQIIAFGGNTIRHEDLEALLGAVPQERIRAMVDAVIQQDS
                     AKALQVIAALLDQGHDVRAYCADLVEYVRNMLVAAVVPSGPELRSLIEATEEDLTQLA
                     RDAERFTVEQLQELFRMYAAAEDSLRVSAHPRFVLETAAVRATRLLRTAEGQPASGGL
                     SVQPNKPGADRRVVTQTPTQSQGKAAQSPAPVANAAGAKGSQDTVAKSSVAGGSAPKA
                     PSAAPLREAAVPSVRPPAVPLSAAAVVPASVAPVQQEPKATSAAEVSAAAVEVNWEQF
                     QEAVSTNHPNIAPFLEMGRLVKIEGGLITLGFAKQATTARSMLEKEDNLQALAALGES
                     LYGCAVRIRIVEVAEQDPGAAPTMKQIRVAKEQEQRLILTQQAKAHPLVKQALEMFGG
                     ELAEVRTTAPAQEVQE"
     gene            1572..1895
                     /locus_tag="PK614_08200"
     CDS             1572..1895
                     /locus_tag="PK614_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRB82230.1"
                     /translation="MKSPFGNMSNILKQAQAMQEQMAKVQEQAATKTVSGTAGGGIVT
                     VTVNGAMDLLSVKIDPEVVKAGDVEMLQDLVVAAGNDALKKSREMMAEEMKAVTGGMK
                     IPGLF"
     gene            1932..2534
                     /gene="recR"
                     /locus_tag="PK614_08205"
     CDS             1932..2534
                     /gene="recR"
                     /locus_tag="PK614_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRB82231.1"
                     /translation="MSVDQQGLLAKLVRELVRLPGIGQKSAQRLAFHLLKAEREDAMR
                     LAEAIQAVKDGLSFCRQCRNIAEGELCEFCRDPKRDRSKILVIEEPSTLYAIERAGGY
                     RGLYHVLLGVLSPLDGVGPSDIRAEELLDRVKAGGVEEVIVATNPTIEGEATAIYLTR
                     LLKPHHVRVTRIAYGIPVGMDIEYADEVTLVKSIEGRRDL"
     gene            2675..>3065
                     /locus_tag="PK614_08210"
     CDS             2675..>3065
                     /locus_tag="PK614_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRB82232.1"
                     /translation="MKTPAKKTTVARRKPPKANGVKYPDILADLEGQRAAILAEAGVV
                     LTNPTGLEVFPDVSDQASAEADQHFSFRIRERERKLLKKIDEALGRFATQTYGICEGC
                     EGDIPYKRLKARPVTTLCIECKTSQEEA"
BASE COUNT          643 a          798 c         1007 g          617 t
ORIGIN      
        1 gacgacgttc gcgagattcg cgagaacgtc aagtttacgc cgttccgggg gcagtatcgt
       61 gtgtacatta tcgacgaagt gcacatgctc tccaattcag cgttcaatgc tctgctgaaa
      121 acgcttgagg agccccccgc gcatgtggtg ttcatcttcg caacgacaga aattcataag
      181 attcccgcga cgatcctgtc acgctgtcag cactacaatt ttcgccggat cgccaaagcc
      241 gagatcgtgc agcggttgcg gcatgtcgcc gatcaagacg ggttgaccat cgaagaccgc
      301 agcctgatgg ctctggcgcg ggccagcgaa ggcagcatgc gtgacgggct cagcttgctg
      361 gatcagatta ttgcgttcgg cggcaacacg attcgccacg aagatctcga agccttgctg
      421 ggtgcggttc cgcaggaacg tatacgggcc atggtcgacg cggtgatcca gcaggacagc
      481 gccaaggcgt tgcaggtcat cgcggcgttg ttggatcaag gacatgacgt gcgtgcctac
      541 tgcgccgatc tggtggaata tgtgcggaat atgctggtgg cggcggtagt gccgtcgggg
      601 cctgagttgc ggagtctcat cgaagccacc gaagaagatt tgacgcagct ggctcgcgat
      661 gccgagcggt tcaccgtcga gcaattgcag gaattgtttc gaatgtatgc cgctgcggaa
      721 gacagtttgc gagtcagtgc ccatccgcgg tttgtgctcg aaacggcggc ggttcgtgcc
      781 acccggctat tgcgaaccgc tgagggtcaa ccggcatccg gcggcctgag tgttcaaccc
      841 aataagccgg gagccgaccg ccgggtcgtg acacagacgc ctacgcaatc tcagggtaag
      901 gcggcacagt ctcctgcgcc cgtcgccaat gccgccggtg cgaaaggtag ccaggacact
      961 gttgcaaagt cgtctgttgc tggtgggagc gcccccaagg cgccctctgc cgcaccgctg
     1021 cgtgaagcag cggttccgtc tgttcgcccg ccggctgttc ccctatcggc tgccgcggtt
     1081 gttccggctt ccgttgcgcc ggttcagcag gaacccaagg cgacctctgc tgccgaggtg
     1141 tccgctgccg cggtcgaagt gaattgggag cagttccaag aggcggtctc tacgaatcat
     1201 cccaatatcg cgcccttcct tgaaatgggt cgtctggtca agatcgaggg tgggttgatc
     1261 acgttggggt ttgccaagca ggccacgact gcgcggtcga tgttggagaa ggaagacaac
     1321 ctacaagcct tggcggcgtt gggagaaagt ctctacgggt gtgccgtacg aattcgaatc
     1381 gtcgaggtgg cggagcagga tccgggggcc gctcctacga tgaaacagat acgggttgcg
     1441 aaggagcagg aacaacgcct gatcttgacg caacaggcga aggcgcatcc ccttgtcaaa
     1501 caagccctgg agatgttcgg cggagagctg gcggaggttc gcacgactgc tccggcgcag
     1561 gaggtacagg aatgaagagt ccattcggga atatgtccaa cattttgaag caagcgcagg
     1621 ccatgcagga acagatggct aaggtgcagg agcaggccgc gaccaagacc gtctcaggga
     1681 cggctggagg cgggatcgtg accgtgacgg tcaatggcgc tatggacctg ctgagtgtga
     1741 agatcgaccc ggaagtcgtg aaggccggcg atgtcgagat gttgcaggat ttggttgtgg
     1801 ctgccggcaa cgatgcgctg aaaaagtccc gcgaaatgat ggcggaagaa atgaaggctg
     1861 tgacgggcgg gatgaagatc cccggtttgt tttgatggcg gcggcgtgct tcccggatgt
     1921 gatgatgcgt tatgagtgtt gatcagcagg gtctgttggc gaagttagtg cgggagttgg
     1981 tgcgtctgcc cgggatcggt cagaaaagcg cgcaacggct ggcctttcat ctgctcaagg
     2041 ctgaacggga agatgcgatg cgcctcgccg aggcgattca ggcggtgaag gacggactgt
     2101 cgttctgccg acaatgccgg aatattgccg agggggaact ctgtgagttc tgccgggatc
     2161 ccaagcggga tcgcagcaag atcctcgtca ttgaagagcc cagcacgttg tacgcaatag
     2221 aacgggccgg gggctatcga ggcctgtatc acgtcctgct cggagtgttg tctccgttgg
     2281 atggcgtcgg accatcggac attcgtgccg aggagttgct ggatcgagtg aaagcgggcg
     2341 gagtggaaga ggtcatcgtg gcgacgaatc ccacgatcga gggtgaagcg accgccatct
     2401 acttgacccg gctcttgaag ccgcatcatg ttcgggtgac ccgcatcgcc tatggcattc
     2461 ctgtcgggat ggatatcgag tatgcggacg aagtgaccct cgtcaagtcg atcgagggcc
     2521 ggagggatct ctgaccggga ttttccggct cgggcatggt gatgcctcgg cgaagcctcg
     2581 aagaaccgat tgaccccttt gatttccggc tgctatagta gaatttctgt atacggtctg
     2641 aagacaatac cggcatctcg attccgagta ttcgatgaag actcccgcga aaaaaaccac
     2701 ggtagctcgg cgaaagccac ccaaggcaaa cggggtcaag tatcccgata tcctggcgga
     2761 ccttgaaggc cagcgtgcgg cgattttggc tgaagccggc gtggtgctga cgaatccgac
     2821 cggtttggaa gtattccccg atgtgagtga tcaggcgtcc gctgaggccg accagcattt
     2881 ctcgtttcgc attcgggaac gagagcgaaa actgctcaag aaaatcgatg aggcgttggg
     2941 gcgatttgcc acgcaaacct acggcatttg cgagggctgt gaaggcgata ttccctacaa
     3001 gcgtctgaaa gcccgccctg tgaccacgtt atgcattgag tgtaagacca gtcaggaaga
     3061 agccg
//
LOCUS       DAORQN010000178         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1047_length_3052_cov_10.805138, whole genome shotgun sequence.
ACCESSION   DAORQN010000178 DAORQN010000000
VERSION     DAORQN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_3052_cov_10.805138"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(702..1433)
                     /locus_tag="PK614_08215"
     CDS             complement(702..1433)
                     /locus_tag="PK614_08215"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB82233.1"
                     /translation="MALATLFLGNPVPLATAEDEAQVRESARLLAVLLDSGRVAIGRN
                     QDLINDPARGDKGFTPEVFAEQMIALFKERTGHDLRNLSTAQVPALAKPLLERLLEES
                     KKTVATYQTVINVPGLKYKGLIPATFGTETGARFQLWSGIYLKQTAPEGLLRNPKNKP
                     DAFEIAALHKMEDPSFPRNGEQIVSEVVDGGKSVRVLLPLFYGKACLSCHGAPKGERD
                     VTGYAREGAQEGTLGGAISVKLPLP"
     gene            2031..2543
                     /locus_tag="PK614_08220"
     CDS             2031..2543
                     /locus_tag="PK614_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRB82234.1"
                     /translation="MNKGLLIALTGVLMGVQAGVVHAVNPDNGPGCGLGKLAWADYKT
                     PKNIAPQVMMATTNGTFGSQTFGISFGTSGCTNDGKVMAGQQTNVFVASTFDTLSEDM
                     ARGGGEHLAALASLMGVPAEQQPTFFALAQQQYAALVAAGELSSVAVVKALQEAMKEQ
                     PVLAQASGLR"
     gene            2706..>3052
                     /locus_tag="PK614_08225"
     CDS             2706..>3052
                     /locus_tag="PK614_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRB82235.1"
                     /translation="MLNVVGLGAIAGVIPVYLGILAALFLGKVLPRTWEGGLVGVANG
                     VLVYLFFDLMHEATEQTGARDVISWLVFLGSLGVSFVGLVALESSQVFGGRSGNRMLS
                     LPYMIAVGMGLHNL"
BASE COUNT          615 a          825 c          886 g          726 t
ORIGIN      
        1 gtggattgag atctgcggtt cgttttttta acgagtccag gctacgcgga cgttgccgta
       61 taggaaagag gcttacacca aatgcgtgtg ctagatttgt gctagaaatt gtcagaaccg
      121 ctcaattccg ccagaaccta tcgacacaat agacagcgcc taaactgagt ccctacaaag
      181 gaaaatcggg gaaaccttcg caaaatcaag ccactgttaa aactgcttgg tatcgactct
      241 caaggctcag acacgggttc aaatcccgtt gggaccacca acctttcccc tgatcctccg
      301 cagggttagc aactttcctg agttccggcc tgacgagggc tgacccctca taggacaaaa
      361 taggcgtggt gtacgtagtt tctagctgcg tcacggcctc gcggagcttc agattccact
      421 ccatctcgca gtgtgaatat tgacacgtca tgaccgcctt ggtgctgtgc cctaagaggg
      481 ccgaacgtac ctccagctcc ccccgaggtt ctaaagccgg gtcgtgaggg ggtggcggag
      541 gtcgttgaaa tggaggtctt ggaccttggc tgccttacag gtccgatgct acggattggt
      601 tagcgctaca gctggtgggc cggcatgcac tattgaaaag tgctcactgt tcgaggcacg
      661 caccttgtgg gacaaacggg cccgcgtgag tgcgtattct ttcacggtaa gggaagtttg
      721 acgctaatgg cgcctcccag ggtgccctcc tgtgcgcctt ctcgcgcata tcccgttacg
      781 tcgcgctcac ccttcggggc cccatggcaa ctcaggcagg ccttgccata gaacaatgga
      841 agaagcaccc gtacactctt tcctccgtcg accacttcgc tcacgatttg ttctccattg
      901 cgggggaacg acggatcttc catcttgtgc agcgcagcaa tctcaaaggc atccggcttg
      961 ttcttgggat tacgaaggag gccttcgggc gcggtttgct tcagataaat gccggaccat
     1021 agttggaatc gagcccctgt ctccgtgccg aacgtggctg gaattaagcc tttgtacttg
     1081 agccctggga cgttgatgac ggtctgatac gtcgcgacag ttttcttgct ttcctccagg
     1141 agccgttcga gcaatggctt tgccagggcg ggcacttgag ccgtggagag atttcgtaag
     1201 tcgtgtccag ttcgctcttt gaacagggcg atcatctgtt ctgcaaagac ctcgggagtg
     1261 aaacccttgt ctcctctagc gggatcgttg atcaagtcct gattccgtcc gatagccaca
     1321 cgacctgaat ccagcagcac cgctaagagc ctggctgact cccgcacctg ggcctcatcc
     1381 tctgccgtgg ccagtggaac cggatttccc aggaagagag tagcgagcgc cataataagt
     1441 acggttcccc aacgacctgc aattgaacac ctcgatgatt tgcggtgatt gagtttcatc
     1501 ctgacaacct cagctccaca catagtctgc cccctttggt cgattggctc ctatcacctc
     1561 accatacctc taaggtccca gtagtatggt gcgtcatcgc cgttgtcgtc aaacctagga
     1621 cagtgcctag tagagcgcag gtctcgaacc ctcggccgtg caatgtagat atgaattcca
     1681 taggggggtg cactacacgt tgattgccga accgttcatc taaccaactt gtaatctctc
     1741 caccagcgcc tgcactgtca acggggcctt cagtcgcagg tccttcgatc tgatcgctca
     1801 agttcaatgt tttccatcga aggatggcgt gctcgagagc cacgcctctt actacctgtg
     1861 ctgcggcact atgtggggag ttcagtcacg agtttggact cttgcgagga aatgcgcact
     1921 tcgattgact gtataaaaaa cacggctaag gtaagggtgg ccgcacagag atgtcggcac
     1981 gatgggcctt gcgagtgtgc ggtgacgcac gagtacagaa aggagatggc atgaacaaag
     2041 gattgttaat cgcgttgact ggggtgttga tgggtgttca ggccggcgtg gtccacgctg
     2101 tcaatccgga caacggaccc ggatgcggat tgggtaaatt ggcctgggcg gattacaaga
     2161 cgccgaagaa cattgcccct caagtcatga tggcgacaac gaacggtacg ttcgggagtc
     2221 aaacgttcgg gatcagtttc ggtacgtccg gctgcaccaa cgacggcaag gtcatggctg
     2281 gccagcagac gaacgtgttc gtcgccagca cctttgacac gctgtctgaa gatatggcga
     2341 ggggcggcgg ggagcatctg gccgctttgg ccagtctgat gggtgtgccg gccgaacagc
     2401 agcccacatt ttttgcgctg gctcagcagc agtatgccgc gctcgttgcg gccggtgaac
     2461 tttcctcagt cgctgtcgtc aaggcgctgc aagaggcgat gaaagaacag ccggtgctcg
     2521 cgcaggcatc gggccttcga tgactctgta gtgcgctcag tctctccgtt tgttcgatcc
     2581 ctgaattctc acggccggcg ggcggcacag gtagcatccg gccgccgtcg gtcacctctg
     2641 gagattcctt agattccatc acaacgatct ttcttccctg ccgccggttc tggtagaagt
     2701 ctgacatgtt gaatgtggtt ggactcggcg cgatcgccgg tgtcattccg gtctatctcg
     2761 ggattcttgc ggccctgttc ctgggtaagg tgctgccgcg gacttgggag ggcgggctgg
     2821 tcggcgttgc gaacggtgtc ttggtatacc tctttttcga tctcatgcac gaggcgaccg
     2881 agcagaccgg cgcacgtgat gtgatttcgt ggctggtgtt tctcgggagt ctgggggtga
     2941 gtttcgtcgg attggtggcg ttagaatcga gtcaggtatt cggcgggcgc tcaggaaacc
     3001 gcatgctgtc tctgccctac atgattgcgg tggggatggg attgcataat ct
//
LOCUS       DAORQN010000179         3050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1049_length_3050_cov_5.123539, whole genome shotgun sequence.
ACCESSION   DAORQN010000179 DAORQN010000000
VERSION     DAORQN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1049_length_3050_cov_5.123539"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..977
                     /locus_tag="PK614_08230"
     CDS             357..977
                     /locus_tag="PK614_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82236.1"
                     /translation="MILAGAFAIVLFVGLIVGDWMYFARLSSDASRYGYGIGRVQDHF
                     AALTVPQLLRTFDQNGFLSLPHGVARVFPEANRMIIRPTHRLFSLRFRTAWPLKGSIE
                     WAPESEGLKMVCTKRAPWSSSILTFLWFSLVGFGTLGFVITYLLQGGLDSLGSVLLGI
                     GVTGVGMIVVAFGLLTITVAYRIENTRLMQVYTELRDVALKSEGAA"
     gene            complement(1167..1523)
                     /locus_tag="PK614_08235"
     CDS             complement(1167..1523)
                     /locus_tag="PK614_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82237.1"
                     /translation="MGTQKDHRQGTGPVESLRDEFREHLHIFYSTLKLAAPYDTVEKA
                     LGALTARLKTLSPEELQQVSTDSSLRWTCFRQAFVESGLSAKHRGIIAGLARSGAALD
                     LPPEFEHLLNLYRPAS"
     gene            1634..>1872
                     /locus_tag="PK614_08240"
     CDS             1634..>1872
                     /locus_tag="PK614_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82238.1"
                     /translation="MRYRSLVVLIGVLVTGLTGCSSHHHGSYGHGKGDYYWSKASQDV
                     NGLVEKHVKNPETAKQVNAVMGEMITEIKSAREQHR"
     assembly_gap    1873..1886
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1887..2102
                     /locus_tag="PK614_08245"
     CDS             <1887..2102
                     /locus_tag="PK614_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82239.1"
                     /translation="YAATPEEFTKILDELNNNRMRSSARMLSLRFKMKSLLTAEEWSA
                     LSEELKRYGSRYYGKGAEAAAPAAAQP"
     assembly_gap    2268..2277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2278..2737)
                     /locus_tag="PK614_08250"
     CDS             complement(<2278..2737)
                     /locus_tag="PK614_08250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRB82240.1"
                     /translation="MPIVLLIRHGETEWNRSGRVMGDQPIPLNRTGEEQARTCAGILS
                     RTPIAAIYTSPVLRAAQTAEILRGPQEIPLHQVPGLSEIGVGNWINRYWHEFADDPAK
                     REWYTHPDRARPSGGETLREVQQRAVAAVEQALAPVYDTPVVIVSHGDVIR"
     gene            complement(2756..>3050)
                     /locus_tag="PK614_08255"
     CDS             complement(2756..>3050)
                     /locus_tag="PK614_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82241.1"
                     /translation="NLANQFISTLALHPTDPNIVYIGGPEGILKTVDGGKTWQAHNQG
                     LTTMNVRTISLSPSDPQLLYLGTNGSGLYRSINGGAQWTPIPLTSHAQAAPAP"
BASE COUNT          551 a          829 c          987 g          659 t
ORIGIN      
        1 gcgcacgcac gcttcgcgcg actcgtggaa gatttttccg aaaatatgat cgtagacgcc
       61 ggaataactg gtgtacactc gctcgacttt ggcgatatcc atcgtccctc cgcaaaatca
      121 cgcctctccg cccgcacact ctgcagtgca gaagcgacgg cgatgagaag tgtggcgtgg
      181 aaaaatctat gaaggcggga ctgtaatccg gatgactcgt cctcacccgc cggacaagaa
      241 cgggcttaat gtagccaaag cactcatagg gagtcaactg gttcctcggg tttttgagcg
      301 ctcctgcatc tcgtccgggc ttgattctcc gtaaagccct gtgggaagtt tccactatga
      361 ttttggccgg tgcgtttgcc atcgtgttgt ttgtcgggtt gatcgtcggt gactggatgt
      421 actttgcccg tctctcgtcc gatgccagcc ggtacgggta tgggatcggg cgggtccagg
      481 atcactttgc tgcgctgacc gttcctcaac tgctgcgcac gttcgaccag aacggttttc
      541 tgagtctgcc ccacggcgtc gcgcgggtct ttccggaggc aaaccggatg atcatcaggc
      601 cgacccatcg tcttttctcc ctgcggttcc gcacggcctg gccgttgaag ggctccatcg
      661 aatgggcgcc ggaatcggag ggactgaaga tggtgtgcac gaagcgggcg ccctggtcgt
      721 cctcgattct cacgtttctg tggttcagcc ttgtggggtt cgggacgctc gggttcgtga
      781 ttacctatct gctccaaggc gggctcgatt cactggggag cgtactgttg ggcatcggcg
      841 tcaccggtgt gggaatgatc gtggtcgcct tcggcctgtt gacgattacg gtggcctatc
      901 gcatcgaaaa tacgagactg atgcaggtct atacggaact tcgtgacgtg gcgttgaaga
      961 gtgaaggggc ggcctagccc gcattattca tgacggttcg tcgcgagatt gcggagtcgc
     1021 gacgcgagac gggcgcgcgg agccgtgagg aagggacgca tccttgagca ggatgtcgac
     1081 cgaccgagcg gcgagcccgc ccgccgatag gcctgtcgca gctgcgaatc cgcgattgca
     1141 gcaaaagcgc tcatgaataa tgcgggctag ctggccggac gatagaggtt gagaaggtgc
     1201 tcgaattcag gcggcaggtc cagtgccgct ccggatcgtg ccaggccggc gatgatcccg
     1261 cgatgtttcg cgctcaggcc ggattcgacg aaggcctgcc ggaagcacgt ccatcgcaag
     1321 gacgagtccg tggatacctg ttggagttct tctggcgata acgttttgag tctcgccgtc
     1381 agggctccca gggctttctc gaccgtgtcg tagggcgcgg ccagcttgag cgtgctgtag
     1441 aatatgtgga gatgctcccg gaattcgtcc cggagcgatt ccaccgggcc tgttccctgc
     1501 cggtggtctt tctgtgtgcc catgtcggtc tcctgtgcgg tgccctgttc aaaggaagct
     1561 tacgggagga acgacgtgcg gcgcaacgcg ttttcgtgcg aggcattcat ttttcttatg
     1621 tatggaggct gtaatgcgtt atcgatcctt ggttgtcctc atcggcgtgc ttgtgaccgg
     1681 attgaccggg tgttcatccc atcaccacgg gagttatgga catgggaagg gcgactatta
     1741 ttggagcaag gcttctcagg atgtgaacgg cttggttgaa aagcatgtca aaaatccgga
     1801 gaccgcgaag caggtcaatg cggtgatggg agagatgatc acggagatca agtcggcacg
     1861 ggagcagcat cgnnnnnnnn nnnnnntatg cggcgacgcc ggaggagttt acgaaaattc
     1921 tggacgagtt gaacaacaac cggatgcgtt cgtccgcacg gatgctcagc cttcggttca
     1981 agatgaagtc gttgctgaca gccgaggaat ggtcggcatt gtcggaggaa ttgaaacggt
     2041 atggcagccg gtactacggc aagggcgcag aagctgcggc ccctgctgcc gcccaaccct
     2101 agaaggtagg gtggtcgact acagaagcgt ttctaagccg gggcgatggt tgaggaacag
     2161 tagttgggct gcggtccccg tcaggtccag cccgctgacc gccacgtgat cgatgcgagc
     2221 ctggcggatg atcgccaggt cgaggtgcag gtagtgcgcg agaatcgnnn nnnnnnnccc
     2281 gaatgacatc cccgtgtgac acgatcacaa cgggggtgtc gtagacgggg gcgagggcct
     2341 gttccactgc cgccaccgcc cgctgttgca cctcgcgcag cgtttctccg ccggaaggcc
     2401 gggcccggtc cgggtgggtg taccactccc gcttggccgg gtcgtcggcg aattcatgcc
     2461 aataccgatt gatccaattc cccacgccga tctcactcag tcccggaacc tgatgtagcg
     2521 ggatctcctg tggcccacgt agaatctcgg cggtctgcgc ggcccgcagg accgggctgg
     2581 tgtagatggc cgcgatcggg gtgcgagaca ggatgccggc gcaggtgcgg gcctgttctt
     2641 cgccggtccg gttgagggga atcggttgat cgcccatcac ccggccggat cgattccact
     2701 ccgtttctcc gtgtctgatg agcaggacga tcggcatcgg gagatctcac caatcctacg
     2761 gcgctggcgc cgcttgcgcg tgactggtca gggggatcgg ggtccactgt gcgccgccat
     2821 tgatcgaccg atacaacccg ctaccattgg tccccagata gagcagttga ggatcgctgg
     2881 ggctgaggct gatcgtccgc acattcatgg tcgtcagtcc ctggttgtgg gcctgccagg
     2941 ttttcccgcc atcgacggtc ttgagaatac cctccgggcc accgatgtac acaatgttgg
     3001 gatcggtcgg atgtagcgcc agggtgctga tgaattgatt ggccaggttc
//
LOCUS       DAORQN010000180         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1050_length_3049_cov_4.025718, whole genome shotgun sequence.
ACCESSION   DAORQN010000180 DAORQN010000000
VERSION     DAORQN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_3049_cov_4.025718"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>656
                     /locus_tag="PK614_08260"
     CDS             <1..>656
                     /locus_tag="PK614_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82242.1"
                     /translation="EWGPVDGALRLLRARVHLDWGAWVGLTPSGLVNTMTLDLSRKPG
                     WQRLRADDLRGMAAQAMRVPLEEVRFFYNDQDVTIGATGAATIRHRKDAIYFLPDGTF
                     DSKQFMACMGAMHWEEIDFLPVVELFLSLLPGTGSALFELIRGLYDDQNRTKPAPRAL
                     RYRGIPTYPSEAAYRLFSNFFSPQIPGGGDPFPLFMDPPRSHMVTWLPVPDPPRRHVD
                     A"
     assembly_gap    657..715
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <716..1596
                     /locus_tag="PK614_08265"
     CDS             <716..1596
                     /locus_tag="PK614_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82243.1"
                     /translation="TCWDDPAGLSYQPFDRCGAAPCERGVAVEQGQLLLVDRGTRRVL
                     PLGHSWGTVTGPPMVGAARVGIDWTAVFGGAAPVVSPDEAFGAVLLYPDDEREIGELP
                     TQPFVADYLQDLIEQDRPLAAQVGRAGHQLISGFDAAMIICIVWDSTVFYTVLYDHPA
                     FAQRQAQMLWNLWARAQRLDWLSHVRLSARVDERGDLNNRTYDLAYEWTPFSHYDKPD
                     AVAARMQQLARQLLPGAVVFIVGPSAVADGCRAAGMHVQAITPVASLPTFRMHQSVLP
                     RAQLKPGVMLFQIARP"
     gene            1730..2263
                     /gene="gspG"
                     /locus_tag="PK614_08270"
     CDS             1730..2263
                     /gene="gspG"
                     /locus_tag="PK614_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRB82244.1"
                     /translation="MGWRHYLNSSGSRRTGTVTDSWGTQLNRLRSCGGFSFIEVMIVV
                     VILAILATLLIPRVMGRTEDAKRAAAKAQISNIDSALQLYKLDNGNIPTTEQGLKALV
                     ERPASGPAAPNWKAGGYLPKVPVDPWGGPYKYTTPSTQGGEFEIISFGADGVPGGEGK
                     NADIVSWDLAVTLSLPQ"
     gene            2381..>3049
                     /locus_tag="PK614_08275"
     CDS             2381..>3049
                     /locus_tag="PK614_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRB82245.1"
                     /translation="MRRVIFVDDAPEILKHLQRTLAPMQAEWTIEFYPSATAALAAIR
                     ETPCDVVVSDMTMPGMDGAQFLGEVRKVCPQATRIVLSGDQSPANYLRSASVAHRFLQ
                     KPFDVATLKATIEKAEALRDVLANPALRSLANEIKTLPSLPSIYQELMKEMQAPQASL
                     KKASRIVAKDLGMVTKILQLVNSAFFGLRTHVSDPEQAVALLGFDTIKSLVLSSQVFA
                     QFDQA"
BASE COUNT          530 a          872 c          974 g          614 t
ORIGIN      
        1 gaatgggggc ccgtcgatgg cgcgctccgc ctcctgcggg caagggtgca tctcgactgg
       61 ggggcctggg tcggcctgac tcccagcggc ctggtgaata ccatgaccct ggacttgtcg
      121 cgcaagccgg ggtggcaacg actgagggcg gacgatcttc gtggcatggc ggcgcaagcg
      181 atgcgggttc cgctggagga agtccggttc ttctacaacg atcaggatgt caccatcggg
      241 gcgacgggcg ccgccacgat ccggcaccgg aaggatgcga tctatttttt gccggacggg
      301 acgttcgact ccaagcaatt catggcttgt atgggagcca tgcattggga ggagatcgac
      361 tttcttccgg tagtggagct gtttctctcg ctgctgcccg gcactgggtc ggcgctgttt
      421 gaattgattc gcgggctgta tgacgatcag aaccggacga aacccgcgcc acgggctctg
      481 cggtatcgag gcattcccac ctatccgtcc gaggcggcct accggctgtt cagcaacttt
      541 ttctccccgc agatccccgg cgggggcgat ccctttcccc tgttcatgga tcctccccgg
      601 tcgcacatgg tgacctggct cccggtgccg gacccgccta gacggcatgt cgatgcnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccacc
      721 tgctgggatg acccggccgg actctcctat caaccgttcg accggtgcgg cgcggcgccc
      781 tgcgagcgtg gagtggcggt tgaacagggg cagctcctgt tggtggatcg aggtacccgc
      841 cgggtgcttc cgttgggcca cagctgggga actgtgaccg gaccgccaat ggtcggcgct
      901 gctcgggttg gtatcgattg gactgctgta ttcggagggg ccgcgccggt ggtgagtccg
      961 gatgaggcgt ttggcgcggt gctgctctat ccggacgatg aacgtgagat cggtgaattg
     1021 ccgacgcagc cgttcgtagc ggactatctg caggacctca tcgagcagga tcgcccgttg
     1081 gctgcgcagg tagggcgggc agggcatcag ttgatcagcg gattcgacgc ggcgatgatt
     1141 atttgtatcg tatgggatag caccgtcttc tacaccgtgc tctacgacca cccggcgttc
     1201 gcgcagcggc aggcgcagat gttatggaac ctgtgggctc gcgcacaacg attggactgg
     1261 ctctcccatg tgcgcctgag cgcccgtgtc gatgagaggg gagatctgaa caacaggacg
     1321 tacgatctcg cctacgagtg gacaccgttc agccactatg acaaaccgga tgcggtggct
     1381 gcgcggatgc agcaacttgc ccggcaactc ctgcccggcg ccgttgtgtt tatcgtcggc
     1441 ccttcagcgg tagcagacgg atgtcgagcc gccgggatgc acgtccaggc gatcacgccg
     1501 gtggcatcgc tgccgacgtt tcggatgcat cagagcgtgc ttccccgcgc gcagctgaag
     1561 cccggcgtca tgctctttca gatcgcgcgg ccctgagcgg attcgcgatg gcaggggtcg
     1621 caggcattgg gctacagggg gggcgaagtt gctgttgcaa ttcccgctcg gcatgagtct
     1681 gcgtggtaca aggtcctcca ggtctgtcga tagaaggagc agcgtgcata tgggatggcg
     1741 tcactatctg aatagttcag gctctcgtcg taccgggacg gtgaccgatt cttggggcac
     1801 tcagctcaat agactgagat cctgcggagg attttccttc atcgaggtca tgatcgtcgt
     1861 ggtgatcctc gcgatcctgg cgacgctgtt gattcctcgc gtgatggggc gcacggaaga
     1921 tgccaaacga gccgcggcca aggctcaaat ttcgaatatt gacagtgccc ttcaactcta
     1981 caagctcgac aacggcaata ttcccacgac cgagcagggc ttgaaggccc tggtcgagcg
     2041 acctgcgtca ggtcctgctg cccccaactg gaaggccggc gggtaccttc ccaaagtgcc
     2101 ggtcgatccc tggggcggtc cctacaaata tacgactccc tccacgcagg gtggcgagtt
     2161 cgagatcatt tccttcgggg ccgacggggt cccgggaggc gaagggaaaa acgccgacat
     2221 cgtaagttgg gatctcgccg tcaccctctc tctgcctcag tagttgtcac gaatcattcg
     2281 cgcacgccgc cttccttccg ggctttcctg aaccgactca aggttctggc atggctgacc
     2341 gatgagaaga ggaggaagat cctgccggga ggaggggacg atgcgacgtg tgatctttgt
     2401 ggacgatgcg ccggagattt tgaagcattt gcagcggaca ctggctccga tgcaggccga
     2461 atggacgatt gagttctatc cctccgccac ggccgcattg gccgccatcc gtgagacccc
     2521 gtgcgacgtg gtggtgtcgg acatgacgat gccggggatg gacggcgctc aatttctcgg
     2581 cgaggtgcgc aaggtctgtc cacaggcgac ccgcatcgtt ctgtccggag accagagtcc
     2641 tgccaattat ctgcggtccg cgtcggtcgc acatcgattt cttcagaaac cgttcgatgt
     2701 cgccacgctg aaagcgacca ttgagaaggc ggaggcgtta cgggacgtgc tggccaatcc
     2761 cgcgctgcgc agcctggcga acgagatcaa aacgctgccg agtctcccgt cgatctacca
     2821 ggagttgatg aaggagatgc aggccccgca ggcctcgttg aaaaaggcgt cccgcattgt
     2881 ggccaaagat ctcggcatgg tcacgaagat cctgcagctg gtgaattccg ctttcttcgg
     2941 tcttcgcacc catgtgtccg atccggagca ggcggtggcg ctgctcggat tcgatacgat
     3001 caagtcgctg gtgctctcca gtcaggtctt cgcgcagttc gaccaggcg
//
LOCUS       DAORQN010000181         3031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1056_length_3031_cov_10.473118, whole genome shotgun sequence.
ACCESSION   DAORQN010000181 DAORQN010000000
VERSION     DAORQN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1056_length_3031_cov_10.473118"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1373)
                     /locus_tag="PK614_08280"
     CDS             complement(96..1373)
                     /locus_tag="PK614_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB82246.1"
                     /translation="MALPYEIFIGLRYLRAKRRNRTISFNTIVSIAGITLGVAALIGT
                     VGIMTGFKEDVQAKILGTTAHILVNDRMKESMVDYEDQVKKVAAVPDVVAATPFIFRQ
                     VLLTSPSGVQGIILRGIDPAREGTVTELAHNLVNSNLDDLARQNKVVIPEKEREPNGP
                     DTAMRPGIILGKELSMRLGAFPGDTLNVVSPVGPVTGASMTPKIRQFVVVGIFQSGMY
                     EYDSSLAYIELGEAQKFFNMGASVTGIEVKVTDVFRAADIARAIEHHLGFAFWARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQREIAILKAMGATRK
                     GIMRIFMLNGLIIGCSGAAIGVPLGYTFLWLIQTFWTFDPTVYYISRIPVHVLGSDVL
                     LVAGSAILISFVATLYPSLQAAKLDPAAALRYE"
     gene            complement(1376..2857)
                     /gene="lysS"
                     /locus_tag="PK614_08285"
     CDS             complement(1376..2857)
                     /gene="lysS"
                     /locus_tag="PK614_08285"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRB82247.1"
                     /translation="MDELNDQHQQRIKKLDHLRQLGVAPYGTRFEVKDRAGQLTRLHG
                     EKTKDVLELEQISCTIAGRIVGLRRFGKAAFAVLQDGSDRLQVYLKKDTLGEQAHQIS
                     EGLDLGDWIGITGVLFRTKTNEFTVEVKTLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPQVHQIFALRSRIVSGIRAFLIERGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LDAELYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADY
                     HDLIVLTEELFGRLAQDILGTTTIDYQGTQINLGSPWRRWSYHQSILEVNSLPPSVLT
                     NRDEALAAAQHLGVEVSPKESLVNILNEIFEETVEPRLQQPTFITDYPIEISPLARRK
                     DSDPSLTDRFELYIAGREIANAFSELNDPLDQRERFEAQAAKHAAGDEEAHRVDEDFL
                     RALEYGMPPTAGEGIGIDRLVMLFTNQSSIRDVVLFPQLRPEK"
BASE COUNT          607 a          814 c          940 g          670 t
ORIGIN      
        1 aaacgatttg tagagatcga cgacgtcaat catgtcggtg ctgcgatctg taatggttca
       61 cgttcaatcg tactgcccgg aatgcccgga ctctcttatt catatcgcaa ggccgccgcc
      121 ggatcgagtt tggccgcctg gagtgacgga tagagcgtgg cgacgaagct gatcagaatc
      181 gccgaaccgg caaccagcaa cacatctgaa ccgagcacat gcaccggaat gcgggagatg
      241 tagtacacgg tcgggtcgaa ggtccaaaag gtctggatca gccacagaaa tgtatacccc
      301 agtgggacgc cgatcgccgc accggaacag ccgatgatca gaccattgag catgaaaata
      361 cgcatgatgc ccttacgcgt cgcgcccatc gctttgagga tcgcgatctc gcgctgcttt
      421 tccgtcacga tcatggtgag ggtactgacg atattgaagg aggccacgat ggtgatcaag
      481 accagcagga ggaacatcat cgtcttttcc agtttgagcg ccgaaaacag attgcggttc
      541 atctgcatcc agtctctggc ccagaaggcg aatccgagat ggtgctcgat cgcgcgggca
      601 atgtccgccg cgcggaacac atcggtcact ttgacttcga tacccgtcac gctggcgccc
      661 atgttgaaaa acttctgcgc ctctcccagt tcgatgtagg cgagggagga gtcgtattcg
      721 tacatgccgg attgaaagat gccgacgacg acgaattgcc ggattttcgg cgtcatgctg
      781 gcgcccgtga ccgggccgac gggggagacc acattcaggg tgtcgccggg gaaggcgccg
      841 agtcgcattg agagttcttt ccccagaatg attcccggcc gcatggctgt gtcgggaccg
      901 ttcggctccc gttctttttc gggaatgacg actttattct gacgcgcgag gtcgtcgagg
      961 ttgctgttga cgaggttgtg ggccaactcg gtgacggtgc cttcccgcgc aggatcgatc
     1021 ccgcgaagaa taatgccctg gacgccggac ggagaagtga gcagaacctg gcggaagatg
     1081 aacggggtgg cggcgacgac gtccgggaca gctgcgacct tcttcacctg gtcttcgtag
     1141 tcgaccatgg attccttcat acggtcgttt accaggatgt gcgcggtggt gccgagaatc
     1201 ttggcctgca cgtcctcctt gaacccggtc atgatcccga ccgtgccgat caaggcggcg
     1261 acgccgagag taatcccggc aatcgagacg atggtgttga aggagatggt gcggttgcgt
     1321 cgcttcgcgc gcaggtaccg caatccgatg aagatttcgt agggcagcgc catgtctatt
     1381 tttcaggcct cagctgcggg aagaggacca catcccggat cgaggactga ttggtgaaga
     1441 gcatgaccag ccgatcgatg ccgatccctt cgcccgcagt aggcggcatg ccgtattcga
     1501 gggcgcgcag gaagtcttca tcgacgcggt gggcttcctc gtctccggct gcatgtttcg
     1561 ccgcctgagc ctcgaagcgc tcgcgctgat cgagcggatc gttcaactcg gagaaggcgt
     1621 tggcgatttc acgccccgcg atgtagagct cgaaccggtc ggtgagcgac ggatcggagt
     1681 cttttctgcg tgcgagcggg gaaatttcga tcgggtagtc ggtgatgaag gtcggctgtt
     1741 ggaggcgcgg ctcgacggtc tcttcgaaga tctcgttgag gatattcacc agtgattctt
     1801 tgggagagac ttcgaccccg aggtgttggg ccgccgcaag agcctcatcg cgattcgtga
     1861 ggacggaagg cggcaggcta ttcacttcca gaatcgattg gtgatacgac catcggcgcc
     1921 acggcgatcc cagattgatc tgtgtgcctt gatagtcgat ggtggtggtg ccgagaatgt
     1981 cctgggccaa ccggccgaag agttcttcgg tcagcacgat caagtcgtgg tagtcggcgt
     2041 aggagacgta aaactccaac atggtgaatt caggattgtg aatcgtcgag atgccttcgt
     2101 tccggaaatt ccggttgatc tcgaacacgc gcgggaagcc gccgacgatc agtcgcttga
     2161 gataaagttc cggagcaatg cgaagataga gctcggcgtc cagggcattg tgatgggtga
     2221 cgaagggttt tgcggcggcg ccgcctggaa tcgggtgcat catcggcgtt tcgacttcga
     2281 gaaagccgcg ttcgatcaga aacgcgcgga tcccggacac gatgcggctg cgcagcgcga
     2341 atatctggtg tacctgggga ttggcaatga gatcgacgta gcgctgccga taccgggttt
     2401 cgacatcggt gagcccatgc cacttttccg gtaacgggcg tagcgctttg ctcagaaacg
     2461 tgagggtctt cacttcgacg gtaaattcat tggtcttggt gcggaacaag acacccgtga
     2521 ttccgatcca gtcgccgaga tccagtccct cggaaatctg gtgagcctgc tcgcccaatg
     2581 tgtccttctt cagatacacc tgcagacggt ccgacccatc ttgcagtact gcaaaggcgg
     2641 ccttaccgaa ccggcgtagt ccgacgatgc ggccggcgat ggtgcagctg atctgctcca
     2701 gctcgagtac gtccttggtt ttttcaccgt gcagacgcgt gagttgtccg gcccggtcct
     2761 tcacctcgaa gcgcgtgcca tatggagcca ccccgagttg ccgaaggtgg tcgagtttct
     2821 tgatgcgttg ttggtgttga tcgttcagtt catccatgat cacacggagc gtatggctgt
     2881 tggcgaatag ctgatggtcc ggaacaggct ccgtttcctg ctatctgcta taggccatag
     2941 gctatcagct ccctttcttt acaggtcatc atccttcatg gcaaccggct ccggggcttt
     3001 cccgccggtc agttttcttt ggagataggc t
//
LOCUS       DAORQN010000182         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1067_length_2985_cov_6.864164, whole genome shotgun sequence.
ACCESSION   DAORQN010000182 DAORQN010000000
VERSION     DAORQN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_2985_cov_6.864164"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..522
                     /locus_tag="PK614_08290"
     CDS             262..522
                     /locus_tag="PK614_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HRB82248.1"
                     /translation="MRKSIGVLLIFILIGGMLGGIFGEILRVMAPNGAIQNIFASNFS
                     PGINPPLTVDLVLLKLTFGFSIKVSLLSVLGMFLGAYLYKQM"
     gene            complement(561..2558)
                     /gene="uvrB"
                     /locus_tag="PK614_08295"
     CDS             complement(561..2558)
                     /gene="uvrB"
                     /locus_tag="PK614_08295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRB82249.1"
                     /translation="MPPFKLEAPFKPCGDQGQAIEKLTSGILAGKQHQALLGVTGSGK
                     TFTMANVVERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIAKDASINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHDMLVYL
                     EEGMETRREKILAKLVDIQYARNDVDFHRGTFRARGDVIEIFPASSEAKSVRIELFGD
                     VVDAIHEIDPLTGKSLGKLPKIAIYPNTHYLIAPDRYERAITGIEEELDARVAAFKKN
                     GQLLEAQRIEQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGDPPPTLLDYFPKDFLL
                     IIDESHASVPQVGGMYEGDFSRKRTLVDYGFRLPSAVDNRPLKFAEFERMLKQVIYVS
                     ATPGPYELEHAKGEVIEQIIRPTGLMDPLIDVRSAKGQVDNLLAEVRTEAAKGNRVLV
                     TTLTKRMAEDLTEYYHDLGVKVRYLHSDIKTLERAEIIRDLRRGVFDVLVGINLLREG
                     LDLPEVSLVAILDADKEGYLRSHRSLIQTAGRAARNLDGRVIFYGDTITDSMRRAMDE
                     TARRRHIQEAYNEAHGITPESIKKQIPLLDYATGGGNAGQLELAAESVGDYATTGDTE
                     QLIRRLEVEMKAAAKKLEFERAAELRNRIRTLRLKALELAQ"
BASE COUNT          618 a          905 c          794 g          668 t
ORIGIN      
        1 caccactgtt gatacacgtg acacacggcg ccaaacttag ttgcaccaga aatactgtcg
       61 ggcgttctcc ctgcttcacc ctcgagcgcc gagggtgccc tctattctcc caccgatctc
      121 cagaagaggt gctttgcaat cagccacatg catccacaga ggcgtctggc atgcattttg
      181 ttgacatcat tttccgcttc cgatatggtg cctcgagtcg gcacatgcac cccgcccagc
      241 gggaactagg agagatatcg gatgagaaaa tcgatcggcg tgctgctgat ctttatcctg
      301 atcggcggaa tgctgggcgg gattttcggg gaaatacttc gcgtcatggc cccgaatggc
      361 gccattcaaa acatctttgc cagtaatttc tctcccggaa tcaacccgcc actcaccgtc
      421 gatctggtcc tcctcaaact gacgttcggc ttcagcatca aggtgagcct gctcagcgtg
      481 ttggggatgt ttctcggggc ctatctctac aaacagatgt aacagaattt ccggcgcagg
      541 agggacagta gccgccttca ctattgcgcc aattccaacg ccttcaacct caacgttcga
      601 atccgattcc gtagctccgc cgcccgctca aactccaatt tcttggctgc cgccttcatc
      661 tcaacctcaa gacggcgaat caactgctcc gtgtcgcccg tcgttgcata atcgcccact
      721 gactccgccg ccagttcgag ttgccccgca ttcccgccac ccgttgcata gtccagcagg
      781 ggaatctgct tcttgatgct ctcgggggtg atgccatggg cctcattata ggcttcctgg
      841 atatgacgcc ggcgcgccgt ctcgtccatg gcgcgccgca tggaatcggt gatcgtatca
      901 ccgtagaaaa tcacgcgccc atccagattt ctagcggcgc ggcccgccgt ctgaatcagc
      961 gaccgatgcg accggaggta gccctcctta tccgcgtcca agatcgccac taaactgacc
     1021 tcgggaaggt cgagcccttc ccgtaacagg ttgatcccga ccaacacatc gaacaccccg
     1081 cgccggagat cgcgaataat ctccgcgcgt tccagcgtct taatgtcgga gtgaagataa
     1141 cgcaccttca cgccgaggtc gtgataatac tccgtgaggt cttcggccat tcgcttggtg
     1201 agggtcgtca ccagcacccg atttcccttc gccgcctccg tccgcacttc ggccagcaga
     1261 ttgtcgacct gcccctttgc cgatcgcaca tcgatcaggg gatccatcag tcccgtcggg
     1321 cgaatgatct gctcgatgac ctcaccctta gcatgctcca gctcataggg acccggcgtc
     1381 gccgagacgt agatcacttg cttcagcatc cgctcgaact cggcaaactt cagcggccga
     1441 ttatcgacgg cggacggcag ccggaatcca taatccacga gcgtccgttt gcgcgaaaag
     1501 tcgccttcgt acatgccgcc gacctgcggc accgacgcgt gcgattcgtc gatgatcaac
     1561 aggaagtctt tgggaaaata atccaacagg gtcgggggcg gatcgcccgg cgcgcgacca
     1621 ctcaagtggc gcgaatagtt ctcgattccg tggcaatacc ccatggcacg gatcatttcg
     1681 agatcgaact tggtgcgctg ctcgatccgt tgcgcttcca acaactgacc gttcttcttg
     1741 aatgccgcca cgcgggcgtc caattcttct tcgatgcccg tgatcgcccg ttcataccgg
     1801 tccggcgcaa tgagatagtg agtattcggg taaatggcaa tcttcggtaa tttgcccagc
     1861 gatttaccgg tcaacggatc gatctcatga atggcgtcca ccacatcgcc gaacaattca
     1921 atacgcaccg atttcgcttc cgacgaggct ggaaaaatct cgatgacatc gccgcgtgcg
     1981 cgaaaggtgc cgcggtgaaa atccacgtcg ttgcgcgcat actgaatgtc gaccagcttc
     2041 gctagaatct tttcacgccg cgtctccatc ccctcttcca ggtagaccaa catgtcgtgg
     2101 tacacctccg gcgatccgag gccgtagatg caggagaccg aggacacgat cagcacatcg
     2161 ttgcgctgca gcaaggatgt cgtcgccgca tggcgcatct gatcgatcgc atcgttgatc
     2221 gaggcatcct tggcaatgta ggtgtcgctc tgcgggatgt aggcttcggg ttggtaataa
     2281 tcgtaataac tgatgaagta ctcgacggca ttgtgaggga agaactgctt gaattcctga
     2341 tacagctggc cggccagagt tttgttatgc acgaggacga gggtcggctt ctggacgcgt
     2401 tcgacgacat tggccatcgt gaaggtcttg ccggagccgg tgaccccgag caacgcctga
     2461 tgctgcttcc cggccaggat tccggatgtc agcttctcaa tggcttgccc ctgatcacca
     2521 cagggtttga atggagcttc gagcttgaac ggaggcatat tgaatagatc agctttctag
     2581 atctgcatgc tgtagtgctt gaggcggctt gctggactcc cgccggaccg gtagtagatt
     2641 gcgttgcgta tgggctcgta gtttatcacc gatcatcata ccattccggg ccttgggcgt
     2701 cggaaaactt ccatacccag actgtccatg aatcagaaac gacgcactcg accgttcctg
     2761 ctgacggcgg gcctagcaat agtggcgggg cgaccccagc atgatgccac ttccggcaaa
     2821 gtttggaggg cgttacggtt cttacgtcgc agaaggggct agtgaacatt cccagagttt
     2881 cgtatagacc gagccggtgg gacgcagttc acttttcatg aggacgattg cctcgaccgg
     2941 caacatgccg atcgccaccg gccgctccaa caacccgttc tgagc
//
LOCUS       DAORQN010000183         2969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1071_length_2969_cov_6.891558, whole genome shotgun sequence.
ACCESSION   DAORQN010000183 DAORQN010000000
VERSION     DAORQN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_2969_cov_6.891558"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK614_08300"
     CDS             <1..954
                     /locus_tag="PK614_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRB82250.1"
                     /translation="SQPLLDRMEIIRLNGYSEREKREIALRYLWPRRLKEAGLRAEEV
                     NLPEAVLDHIIGRYTRESGVRQLEQMLGRITRKVAVTFADRPADAPASPVDITQPLLD
                     EWLGQERFQPEEARKNLPAGVATGLAWTPTGGDVLYIETTLLPGSHELTLTGQLGDVM
                     QESARAARSYLWSHAESMGLDISRFKRNGVHIHVPSGAIPKDGPSAGITMATALASAY
                     VGKPVRSDTAMTGEISLTGLVLPVGGIKEKVLAAHRAGIRRIILPKANEKDLKEVPQE
                     VRDELTVIPVERIEEVLPAAFNQDAAPSSERGEPLASATAS"
     gene            1143..1562
                     /gene="msrB"
                     /locus_tag="PK614_08305"
     CDS             1143..1562
                     /gene="msrB"
                     /locus_tag="PK614_08305"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRB82251.1"
                     /translation="MPPKIEIGEIVKVTKTDDEWKTLLSPAAYQVLRHEDTERAFTSP
                     LHENHQAGIYHCAGCDLPAYSSEHKFDSGTGWPSFWQPIDPKVVEKRTDSRFFMTRVE
                     VHCAQCGGHQGHVFDDGPRPTGLRYCINGVSLKFVPA"
     gene            1704..2855
                     /locus_tag="PK614_08310"
     CDS             1704..2855
                     /locus_tag="PK614_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82252.1"
                     /translation="MRRPLLGLFLAGTAVLGCSQSSDVISSNLNACLIVTEAEVELAI
                     GTPVTPGLRQNDRQCLYQAKRNPQETVTVELNPGPDGDTKGLFHDQRSKAGHQPVPGV
                     GDGAFTLRSPIGGIQLTFLKADALVTLSLSSSKQPNAQAAVTNLAKVAATRLGGPLRT
                     GPAASTEPGLTATPSQWAGDWYGCPPVGLMYGKGHLALSEGGTWSLTTAVLMPGTVTA
                     DRGRWQAESYQEILHGTYQVAGNDRFSTTGILSVTWDKLAKNQGPNKFDPLLWQSFAA
                     VPRKVAVKRLPPVEPSLVGTWEGSAKFVDRQEEFVWTITPANISEFYRATSQSGRIEK
                     EQDRFRLIVPQSKTPPLFVRVLAQDKLELTDNSGTVSQWNRNEKLLPRC"
BASE COUNT          594 a          935 c          882 g          558 t
ORIGIN      
        1 tcgcaacccc tgctcgaccg tatggaaatc atccggttga atggatacag cgagcgggag
       61 aaacgcgaaa tcgccctgcg gtacctctgg ccgagacggt tgaaggaagc cggtctgcga
      121 gccgaggaag tgaatcttcc cgaagcggtg ctggaccata tcatcggccg ctataccagg
      181 gaatcgggcg tacggcagct ggaacagatg ttgggccgca ttacgagaaa ggtcgccgtg
      241 accttcgcgg atcggccggc cgatgctccg gcgagccctg tggacatcac gcaaccgctc
      301 ctggacgagt ggctcggaca ggaacgattc cagccggaag aggcgcgaaa gaatctgccg
      361 gccggagtcg ccaccggtct ggcctggact ccgacaggcg gtgacgtgct ctatatcgag
      421 acgaccctgc tgcccggcag tcacgagctg acgctgacgg gacagttagg cgatgtcatg
      481 caggagtcgg cacgcgcggc gcgcagctat ctctggtcgc atgccgagag catggggctg
      541 gatatctcgc gcttcaaacg caacggggtg cacatccatg ttccgtcggg cgccattccc
      601 aaagacggcc cctcggccgg gatcaccatg gcgaccgcgc tggcttcggc ctatgtcggc
      661 aagccggtgc gcagcgatac ggcgatgacg ggagagatca gcctgaccgg gttggtgctg
      721 ccggtcggcg gcatcaagga gaaagtcctg gccgcgcatc gcgccggcat ccggcggatc
      781 attctgccga aggccaacga gaaggacttg aaagaagtcc cgcaggaagt gcgcgatgaa
      841 ctcacggtca ttccagtgga acggatcgag gaagtgctcc cggcggcgtt caatcaggat
      901 gccgcgccct catccgaacg gggcgaaccg ctggcgagcg cgaccgcctc gtaagatcca
      961 gggccccggc tgaatagccg gggcccctcg cctttgctcc ctgccttcct gcttctactg
     1021 ccacttcttt tgcagtactc ccacacatcc gcttcccttg cccttccgta atcacttatg
     1081 tcaggatcag ctggatgttc gacccgtgcg cacgtcctgc cacgacgtga aggagagtga
     1141 ccatgccgcc caaaattgaa atcggggaaa tcgtgaaggt gaccaagacc gacgacgaat
     1201 ggaaaacact gctgtcgccc gcggcctatc aggtcctgcg gcatgaagat acggagcgag
     1261 cctttacgag tccgctccac gagaatcacc aggcgggcat ctatcattgt gcgggctgtg
     1321 acctgcccgc ctattcctcg gagcataagt tcgatagcgg caccggctgg ccaagcttct
     1381 ggcaaccgat tgacccgaag gtggtggaga aacgtacgga ttccaggttt ttcatgaccc
     1441 gggtggaagt ccactgtgct caatgcggcg gccatcaagg acatgtcttc gatgacggcc
     1501 ccaggcccac cggcctccgc tactgcatca acggcgtctc cttgaagttt gttccagcct
     1561 gattgcgatc acatactcct gcccgacgca tgacgcaggc cggccactgc agcaaccacg
     1621 gcgtcaccac cggagcgtgg ccgcgcacct tgcaatttgt cgctgaaccc tcctagactg
     1681 cgcatgccat ggcgtacttc tccttgagac gaccgctcct gggcctcttc ctggccggaa
     1741 ctgcagtgct tggttgctca caatcctccg acgtgatctc ctccaatctc aacgcctgct
     1801 tgatcgtcac agaggccgag gtcgaattgg ccatcggaac ccccgtgacg ccgggcctgc
     1861 gccaaaatga tcgccaatgc ctgtaccagg cgaagcgtaa tccccaggaa accgtgacgg
     1921 tagagttaaa ccccggtcct gacggagaca caaaaggtct gtttcacgat cagcgttcaa
     1981 aggccggtca ccagccggtt cccggggtcg gcgacggtgc cttcaccctg cggtcgccta
     2041 tcggcgggat tcagttgacg tttctgaagg ccgatgccct cgtcactctg tcgctctcgt
     2101 cctcgaaaca acccaacgcg caggcggccg tgaccaacct cgccaaggtc gccgcgacca
     2161 gactcggcgg cccccttcgc accggtccgg cggcgtcaac cgagccaggg ttgaccgcca
     2221 ctccttccca atgggccggt gattggtatg gttgcccgcc cgtcggattg atgtatggga
     2281 aaggccatct tgcgctgagc gagggcggca cctggtccct gacaactgcc gtattgatgc
     2341 cgggcaccgt gaccgcagac cggggacgct ggcaggcgga atcctatcaa gagatcctgc
     2401 acggaaccta tcaagtggcc gggaacgacc gcttctcaac caccggcatt ctaagtgtca
     2461 cctgggacaa actcgccaag aatcaggggc cgaacaagtt cgacccgctg ctctggcaat
     2521 catttgcggc cgtcccgcgc aaggtcgcgg tcaagcggtt gccaccggtc gagccctccc
     2581 tcgtgggcac atgggaaggt tccgccaagt tcgtcgatcg acaggaagaa ttcgtctgga
     2641 ccatcacgcc ggcgaacatc tcggagttct acagggcaac ctcgcagtct ggtcgcatcg
     2701 agaaggagca ggatcggttt cgcttgattg tcccccaaag caaaactcct cccctgtttg
     2761 tacgggtgtt ggcgcaggac aagctggagc tgaccgacaa cagcggcacg gtttctcaat
     2821 ggaaccggaa tgagaaacta ctcccgcgct gttgatctcc cgattgacgt caccagcgac
     2881 gccagcttac cccctcggct tgatctgttt ttttctgttc ggtagactgt tggcatgaca
     2941 cgttggctca tggtgacgct gctctgtac
//
LOCUS       DAORQN010000184         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1073_length_2964_cov_5.158817, whole genome shotgun sequence.
ACCESSION   DAORQN010000184 DAORQN010000000
VERSION     DAORQN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_2964_cov_5.158817"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..373
                     /locus_tag="PK614_08315"
     CDS             119..373
                     /locus_tag="PK614_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HRB82253.1"
                     /translation="MQLSTEDFSTFLLLEQARVYTKSTAALRIVRHLPGFWPLFYLGI
                     LIPRPLRDALYDYVARHRYRWMGKTDACRVPSPDQRRRFV"
     gene            517..975
                     /locus_tag="PK614_08320"
     CDS             517..975
                     /locus_tag="PK614_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB82254.1"
                     /translation="MNHLFTASIFVAIAMALSVPSPAVAQNFPPSVTQKIHAAQKQVK
                     TIGLEEYRKVMESPGNALIIDVREPQEFAAGHVPGAVNIPRGLVEFRIWKQVGFPANS
                     DTNRPVYLQCQNGNRASLAAQSLAELGFTNTTAVVMNFDEWQQAGHPLVK"
     gene            complement(1173..2519)
                     /locus_tag="PK614_08325"
     CDS             complement(1173..2519)
                     /locus_tag="PK614_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82255.1"
                     /translation="MERVTGGALFVRLASIIGLLTAGLLLCAPWSLAGTIRVDLGVEQ
                     GPMNHRANGYLVSIEPTDPPMELILPLKPTSFRGNTGYVLSNYDRLKQAGVTEFQLTL
                     GLVFEHRALQIFDINQIGLDGNYEPWLAHVDHVIDQVLQRQLSVIWDIYNEPDLAAVP
                     LNDSERLKEGWRVAYQLIKQRIPGAQIVGPSVSRYRLLKPFLEWSDSQGVFPDVVAYH
                     EYADPSDAIRYVNDLRDYLKAHGLARPISVNEILGQESWTIAGYTAGVIAAYERADIL
                     SAMRSCWPDPQDMSRSYVENTCDNPTLDGLLYVDRKQKRPGWHVYKTYAEMEGVRLYT
                     MTDSPKLHALAALDKAAGTLRLLLGKYEDDSPGDTQVVLKNIGRLFSSQHSGTVHLCA
                     EQIPDVGSGPLEAPVVTLDRALSVENEELSFTLPQFYHSDAYRVVLSLSEQCRASH"
     gene            complement(2588..>2964)
                     /locus_tag="PK614_08330"
     CDS             complement(2588..>2964)
                     /locus_tag="PK614_08330"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRB82256.1"
                     /translation="VLVANKPDDYARVLELMQSKQMPDWEAEREVFGADHAHVGAYLL
                     GIWGLGDGIVEAVAYHHHPDDYKGSGFCAVTAVHVANAMAEAQARPGENPTEVPGLDQ
                     HYLAREQLMPQLPRWRELCIAA"
BASE COUNT          651 a          900 c          806 g          607 t
ORIGIN      
        1 cagaatctgt tgtccccggt cggattgtag ggtgccgaac ttgaacgtct cgtggggatc
       61 gtgatcgatc aaaaagttaa ccctacaatc cgaccgggga caacagattc tgcatagact
      121 gcagctttcg accgaagact tttcgacctt cctcctgctg gagcaggccc gcgtctacac
      181 caaatccacg gccgcgttga gaattgtgcg gcacctcccc ggcttctggc ccctattcta
      241 tctcggcatc ctgatcccac gccccctgcg tgatgcgctg tacgattatg ttgcgcgtca
      301 tcggtaccgg tggatgggca aaaccgatgc atgccgggtc ccctccccgg accagcgcag
      361 acggttcgtg tagtgcctgc gggcagagag tttcgccaca cgcaacgcac gttcgtgcct
      421 tgagccattc acgggaacgg cgtagactcc gctttgtaac gatcgcgccg gcatcgaggc
      481 cgtgccattc tcatacctga tgaagaggaa gaacctatga accacttgtt cacggcatcc
      541 atattcgtcg ccatcgctat ggcgctgtct gttccatcac cggcggtcgc acaaaacttt
      601 cctccgtctg tcacacaaaa aattcacgcg gcgcagaagc aggtcaaaac gatcggcctg
      661 gaggagtatc gaaaagtcat ggagagtccg ggcaacgcgt taattataga tgtgcgcgaa
      721 ccgcaggagt tcgcggccgg acatgtgccg ggcgcggtca acattccccg cggactcgtc
      781 gaatttcgga tctggaagca ggtcgggttt ccagccaact cggacacgaa cagacctgtc
      841 tacctgcaat gccaaaacgg caaccgcgcg tccctcgcgg cccagtcgct tgcggagttg
      901 gggttcacca ataccacggc cgtcgtgatg aacttcgatg agtggcaaca ggcaggccat
      961 cccctggtga agtaagccat cgcctcgcgt gcgatgagcg ttcgtgacgc cactcataga
     1021 tccgccgaca catgccctct tggtttgttt attcaggcag aaattattcc cctctgacat
     1081 ccggcaagta atatcaaatg cgtcgcatcc gttgtgaacg ggcactgcat aaatccagcg
     1141 ccaacagcca gtaagtaggt cggccgttcc gctcaatgcg aagcgcggca ctgttcggag
     1201 aggctcagga cgacccgata ggcatcggaa tgatagaact gaggcagtgt gaacgagagc
     1261 tcctcattct ccactgagag agcacgatcg agggtgacaa caggcgcttc gaggggaccg
     1321 gatcctacat ctggtatttg ctcagcgcag agatgcaccg tcccgctgtg ctgcgaggaa
     1381 aacagacgcc cgatattctt cagcacgact tgtgtgtcgc caggcgaatc gtcctcgtat
     1441 ttgcccaaca agagtcgtaa cgtgccggcg gccttatcca gcgcggcgag ggcatggagc
     1501 ttgggactat ccgtcatcgt gtacagcctg accccctcca tctccgcata ggtcttatag
     1561 acgtgccacc ccggccgttt ctgcttccga tcgacataca acaatccgtc cagcgtaggg
     1621 ttatcacagg tgttttccac gtagctccgg ctcatgtcct gaggatcagg ccaacaggag
     1681 cgcatcgcgc tgaggatgtc cgcccgctca taggcagcaa tcacccccgc tgtataaccg
     1741 gcaatcgtcc aggactcctg ccccaggatc tcgttcactg aaatcggccg cgccagaccg
     1801 tgagccttca aataatcgcg cagatcattc acgtacctga tggcatccga tggatccgca
     1861 tattcatgat aggccacgac gtcgggaaag accccctgcg aatcactcca ctccagaaag
     1921 ggcttgagta accggtatcg actcacgctc ggcccgacga tctgtgcgcc gggaatccgc
     1981 tgcttgatga gctgataggc cacccgccag ccttctttga gtcgttcgct gtcattgaga
     2041 ggaaccgctg caagatcggg ttcattgtaa atatcccaga tcaccgacag ttgtcgctgc
     2101 agcacttgat cgatcacatg atccacatgc gccaaccagg gctcatagtt cccgtcgaga
     2161 ccgatctgat tgatatcaaa aatctgcagg gcgcgatgct cgaagaccag tccgagggtc
     2221 agctgaaact ccgtcacccc ggcctgtttg agccggtcat agttactcaa cacatatccg
     2281 gtgtttccgc gaaaactggt gggcttcaac ggaagaatca gctccatcgg cgggtctgtc
     2341 ggttcgatgc tgaccagata cccgttagct cgatggttca tcgggccctg ctcgaccccc
     2401 agatcgaccc tgatagtgcc ggccagactc cagggcgcgc acaacaacag tccagctgtc
     2461 agcaggccaa tgatcgaggc aagtcgcaca aacagggctc ctcccgtgac gcgttccaat
     2521 cagaaaatcc aacgatgttc ggttgtactg ccggaaaatg ccggcggtca agatggagcg
     2581 ggtagaatca ggccgcgata cagagttcgc gccatcgtgg aagctggggc atcaactgtt
     2641 cacgcgccaa atagtgctga tccagaccgg ggacttcggt cgggttctca ccgggacggg
     2701 cctgggcctc agccatggca ttcgccacat gaacggcggt cacggcacag aagccggatc
     2761 ccttgtagtc gtcaggatgg tggtgataag ccaccgcttc cacaatgccg tcgccgagtc
     2821 cccagattcc gagcagatag gccccgacat gggcgtgatc ggccccgaac acctcccgct
     2881 cggcttccca atcaggcatc tgttttgact gcatcagctc caggactcgc gcgtagtcat
     2941 cgggtttgtt ggccaccaat acca
//
LOCUS       DAORQN010000185         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1079_length_2944_cov_9.732911, whole genome shotgun sequence.
ACCESSION   DAORQN010000185 DAORQN010000000
VERSION     DAORQN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1079_length_2944_cov_9.732911"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /gene="folP"
                     /locus_tag="PK614_08335"
     CDS             <1..845
                     /gene="folP"
                     /locus_tag="PK614_08335"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRB82257.1"
                     /translation="GRSVLNQVHSSWGVLNVTPDSFSDGGAYLTVEHALQHARQMQAE
                     GADIIDIGAESSRPGAQPIDETEELRRLLPVLEAVHDAVPLPISVDTTKAAVARRAIR
                     AGATIVNDISALRGDPLMGSLVAETGAAVVLMHMQGTPQSMQQAPRYGHVVEEVLAFL
                     RERVQVAIAHGIKGSQIVLDPGFGFGKLQEHNLQLLAEFDAFTKLGYPVLAGVSRKQF
                     IGNLTQQPVQERGYGTAGAVAVAVLKGARIIRVHDVRSMRDTVSVVSAISRHVRSHVG
                     VSNA"
     gene            838..2187
                     /gene="glmM"
                     /locus_tag="PK614_08340"
     CDS             838..2187
                     /gene="glmM"
                     /locus_tag="PK614_08340"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRB82258.1"
                     /translation="MRKLFGTDGVRGVANLEPMTSEIAMQLGRAAAHIFMRRAGRHQV
                     VIGKDTRLSGYMLESALTSGICSMGVDVLLVGPLPTPAIAFLTRSLRADAGVVISASH
                     NPYQDNGIKFFSNEGFKLPDELEARIEQLIISDEIKHLRPTADAIGKAYRIDDAEGRY
                     IEFVKRSLPRDLDFQGIKLVVDCANGAAYKVAPAVFRELGAEIEVIANTPDGMNINDR
                     CGAVHPERLQEAVRRHGAHIGIALDGDADRAIFVCEQGQIVDGDHVMAALSLDLHAQG
                     QLACQTVVGTVMSNFGLEIAMKKAGIQLMRTPVGDRYLMERMLADGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQILSLMKRTGTPLSELAKAMTAVPQILLNVQVKHKPDLNQIPD
                     IQQAIKNAETTLNGSGRVLVRYSGTESLLRIMVEGERHSTIREVADHLAEIVRARIG"
     gene            2214..>2944
                     /locus_tag="PK614_08345"
     CDS             2214..>2944
                     /locus_tag="PK614_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRB82259.1"
                     /translation="MLPTLTITFVLGLVLGSYLTYVPLSIGTVLVACTAGCVWLERRN
                     RLTSRQSNLLLACLFGGCLYWALCAWYTPHVPVPDSSGAFPVRLTGTVVEAVRHAPGR
                     LTAMVQVTGSDDPLLPTPYHLRLTWRDPDRDLRRGVQIRTRTHVHAPVGTLNPRGFDY
                     AAYLEALGVDAVGSVSGAGAIEVLDPGRESPLHLLASLIEAWRSRVRAAAESLPQPGR
                     GLFLSLTIGEQGFLDPDVREWFMTTG"
BASE COUNT          548 a          857 c          898 g          641 t
ORIGIN      
        1 tcggacgttc agttttgaat caggtccact cctcatgggg ggtgctcaac gtcacccccg
       61 attcattttc agacggcggt gcctatctca cggtcgagca tgccctgcag cacgccagac
      121 agatgcaggc cgaaggtgcc gatatcatcg acatcggcgc tgagtcttca aggccagggg
      181 ctcaaccgat cgacgaaaca gaagaattac gtcgcctcct tcctgtcctg gaagcggtcc
      241 atgacgcagt gccgcttcct atttccgttg atacgactaa agctgcggtg gcccgtcgag
      301 cgattcgtgc cggtgccacc atcgtgaatg acattagcgc cttgcgcggt gaccccttga
      361 tgggctcgct ggtggctgaa accggcgcgg ccgtggtgtt gatgcacatg caggggactc
      421 cgcaatctat gcagcaggcg ccacggtacg gccatgtcgt ggaagaggtg cttgcattcc
      481 tgcgcgaacg ggtgcaggtc gccatcgctc acggcatcaa gggaagtcag atcgtccttg
      541 accccggctt cggttttggt aagctccaag agcataatct tcaattgctc gctgagtttg
      601 acgccttcac gaagctcggt tatccggttc tggcaggggt ctcccgtaaa cagtttatcg
      661 ggaacctgac tcaacagccg gtgcaggaac gggggtacgg caccgccggc gcggtggcag
      721 tggccgtcct caaaggggcc cggatcattc gtgtacacga cgtgcgatcg atgcgcgata
      781 ccgtctccgt cgtgtcagcc atttcccgtc acgtgcgttc tcatgtaggg gtatccaatg
      841 cgtaaattgt tcggcacaga cggtgttcgc ggggtggcca acctcgaacc aatgaccagt
      901 gaaatcgcca tgcagttggg gcgcgcggcc gcccatattt tcatgcggcg cgcaggccga
      961 caccaggtgg tcatcggaaa ggacacgcgt ttatccggat acatgctgga atcggcgttg
     1021 acgtccggga tctgttccat gggagtcgac gtactcctgg tcgggccctt gcccaccccg
     1081 gcgattgcct tcctcacgcg aagtcttcgg gcggacgccg gagtcgtgat ctctgcgtcg
     1141 cataatcctt atcaggacaa cgggatcaag tttttttcca acgagggatt caagctgcct
     1201 gatgagcttg aggcgcgcat cgaacagctg atcatttccg acgagatcaa acatcttcgc
     1261 ccgacggcgg acgcgatcgg aaaggcctat cgtatcgatg atgcagaagg ccggtatatt
     1321 gagttcgtca aacgctcgct tcccagggat ctcgattttc aaggaatcaa actggtggtg
     1381 gactgcgcca acggtgccgc gtataaggtc gctcctgccg ttttccgtga actcggcgca
     1441 gaaattgaag tgatcgcaaa tactccggac ggaatgaaca tcaacgacag gtgcggtgct
     1501 gtgcatcctg agcgcttgca ggaggcggtg cgtcggcatg gcgcccatat cggtattgcg
     1561 ctggacgggg acgccgaccg ggccattttt gtctgtgaac agggacagat cgtcgatggt
     1621 gaccatgtca tggcggcgct cagcctggat ttgcatgccc aggggcagct cgcctgccag
     1681 accgttgtgg gaaccgtcat gagcaatttt ggactggaaa tcgccatgaa gaaagccggt
     1741 attcagctca tgcgcacgcc ggtcggcgac cggtatctca tggagcgcat gctggccgac
     1801 gggtacaatt tcggcgggga gcagtccggg cattttattt tcctggatca caacacgacg
     1861 ggcgatggct tgatctcggc gttgcagatc ctctcgctga tgaagcgcac gggaaccccg
     1921 ctctcagagc tggccaaggc gatgaccgcg gtccctcaga ttctgttgaa cgtgcaggtc
     1981 aagcataagc cggacctgaa tcagataccc gacatccagc aggccatcaa gaatgcggaa
     2041 actaccctga atgggagcgg ccgcgttctg gtccgatatt ccggcaccga atcgctgcta
     2101 cgcatcatgg tcgagggcga acgtcactca accatccgtg aggtcgccga tcatcttgcg
     2161 gagatcgtgc gcgcccgcat cggatagcat cgggcccatg tcgtaggcgc cggatgttac
     2221 cgactcttac gattacattt gtgctcgggc tcgttctcgg ttcctacctc acgtatgtcc
     2281 cactcagcat cgggacagtc ctggtcgcgt gtaccgccgg ctgtgtgtgg cttgagcgtc
     2341 ggaatcgcct cacgtcacga cagagcaacc tgctcctggc ctgtctgttc ggtggatgtc
     2401 tgtattgggc cctctgtgcc tggtataccc cgcacgtgcc ggtgcctgat agttccggtg
     2461 cgtttcctgt ccggttgacg ggaacggttg ttgaggccgt tcgccatgcg ccgggccgtc
     2521 tcacggctat ggtgcaggtg actggcagcg acgatccact gttgcccaca ccttatcatt
     2581 tgcgtctgac ctggcgagac ccggatcgcg atctccggcg cggggtgcag attcgtactc
     2641 gtacccatgt ccatgcgccc gtgggaaccc tgaatccgag agggttcgac tatgcggcct
     2701 atctggaagc gctgggtgtc gatgccgtcg ggtcggtctc aggcgccggc gccatcgagg
     2761 tgcttgatcc gggccgcgag tcgcctcttc acctgcttgc ctccctcatc gaagcatggc
     2821 gcagccgggt aagagccgcc gccgagtcgc ttccccaacc cggtcggggc ctgtttctca
     2881 gtctaaccat cggcgaacag gggttccttg acccggacgt tcgggaatgg tttatgacga
     2941 ccgg
//
LOCUS       DAORQN010000186         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1096_length_2901_cov_5.892481, whole genome shotgun sequence.
ACCESSION   DAORQN010000186 DAORQN010000000
VERSION     DAORQN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_2901_cov_5.892481"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..>734
                     /locus_tag="PK614_08350"
     CDS             193..>734
                     /locus_tag="PK614_08350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRB82260.1"
                     /translation="MNRISSTPVEIVVTGGESSKRIDVFLANRDPTFSRSALQRLIGE
                     GRILINGQTIRPSQKIKPGDRIRLEVPRPEPLDLKPEAIPFDILHEDADLLVLHKPAG
                     LVVHPAPGNWSGTLVNALLHHFATSGVTPSHIGGKERPGLVHRLDKETSGVMVIAKTD
                     QAHRALAAQFKLHTITRVYEA"
     assembly_gap    735..744
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <745..1143
                     /locus_tag="PK614_08355"
     CDS             <745..1143
                     /locus_tag="PK614_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB82261.1"
                     /translation="GHGLIELAIGRDTKERKKFSARTTKPKPSATDYRVDERYGKIAA
                     HVRLFPRTGRTHQLRVHLTSIDHPILGDKTYGGAKVMSVAGVPIPRVMLHARTLGFTH
                     PTGGEYHQFDVPCPPDMEQVQDLLRAATAH"
     gene            1181..1645
                     /locus_tag="PK614_08360"
     CDS             1181..1645
                     /locus_tag="PK614_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82262.1"
                     /translation="MPNMTVGESEERRTMETSAVLDRIGELIAQLKGYASKLPPVVHL
                     ASVPTGVKPKPEAVEVFEQALLRFREQSGRSPFKSLQDYLIESLEAFEVGNVLGVVQP
                     LLAVLDQVERMHRDREIKVGPAEEKRMGEYRAALVKILPGSQPELEGAGRGM"
     gene            1947..2390
                     /locus_tag="PK614_08365"
     CDS             1947..2390
                     /locus_tag="PK614_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB82263.1"
                     /translation="MPPALKTDQHPITFSDRNDFDAAQLIHLYRQAPWAKHRALEQAQ
                     AMLAKTDVVISAWDGTRLVGFGRVLTDYVFRASIWDVIVDREYQKRKIGTEIVRRILD
                     HPTLQHVELFWLCTRMPGFYERLGFSAKEQTGMVWSRKKSEPQAS"
     gene            complement(2496..>2901)
                     /locus_tag="PK614_08370"
     CDS             complement(2496..>2901)
                     /locus_tag="PK614_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRB82264.1"
                     /translation="LLVLGTILLLSKELYFIALYQDMSVASGVPARQIFYLLLTLVAL
                     TVVIALKTVGAILVFAMLLIPASTAYQLTHSLTQMTLYSMLIGIFCAVSGVLLSYAFD
                     LPSGPSIVLLATSLFFLAVCCSPKRLHRIQPT"
BASE COUNT          619 a          794 c          907 g          571 t
ORIGIN      
        1 gaaatgaaca ggcaaacgta cattcgtgcc ggcgctgaag gatgcgtgtg tgccgcgcaa
       61 catgtggatg gattgtggac gacatgtgtg tgaggtgtgc agaggatggg gatggagcgg
      121 gcaccttgtg tttgaccggc ctctgtggca gcctatgccc acgcatgtcg caggatgtca
      181 ggcagtcagc acgtgaatag gatcagtagc actccggttg aaattgtcgt gaccggagga
      241 gaatcgtcca agcggatcga tgtcttcctt gccaaccgcg acccgacctt ctctcgttcg
      301 gccttgcagc gcttgatcgg agaagggcgc attctcatca atggccagac gatccggcca
      361 agtcagaaga tcaaaccggg tgaccgcatc cggctggagg tgccgcggcc tgaaccgctg
      421 gatctcaaac cggaggccat cccctttgat attctgcacg aagatgcgga cttactcgtg
      481 ctccataagc cagccggcct cgtggtgcac ccggcgccgg ggaattggtc cgggacgtta
      541 gtgaacgccc tcttgcatca ctttgcgacc tcgggtgtca ccccgtcgca tatcggaggt
      601 aaggaacgcc caggactcgt tcaccgattg gacaaagaaa cctcgggcgt catggtgatt
      661 gcgaaaacgg accaggcgca tcgcgcgctg gccgcgcagt tcaagctcca cacgatcacc
      721 agggtgtacg aagcnnnnnn nnnngggcat ggcctcattg aattggcgat cgggcgcgat
      781 accaaagagc ggaaaaagtt ttcggcgcgg acgaccaaac cgaaaccctc ggcgaccgac
      841 tatcgggttg atgagcggta cggcaagatc gcggcccatg tgcgcctctt ccccagaacc
      901 ggacgcaccc atcagctgcg ggtccatctc acgtcgatcg atcatcccat cctgggggat
      961 aagacatacg gcggggccaa agtgatgtcg gtggcggggg tgccgattcc ccgtgtcatg
     1021 ctccacgcgc gtacgctggg atttacgcat cccaccggcg gggagtacca tcagttcgat
     1081 gtgccctgtc cgcctgatat ggagcaggtg caggatctcc ttcgcgcagc cacggcgcat
     1141 taggagtccc gggcgatcgg cccgagccag attgaagaac atgccgaaca tgacggtcgg
     1201 cgagagtgag gagcgacgaa cgatggaaac cagtgccgtg cttgatcgga tcggtgagct
     1261 gatcgcgcag ctgaaagggt acgcctcaaa attgccgccg gtggtacatc tcgcgtcggt
     1321 gccgaccggg gttaaaccga aacctgaggc ggtggaagtg ttcgagcagg cccttctgcg
     1381 gtttcgggag caaagcggcc gctctccgtt taagagtctg caggattatc tgatcgagtc
     1441 gctcgaagcg ttcgaggtcg gcaatgtgct gggcgtggtt caaccgcttc tggccgtcct
     1501 tgatcaggtg gagcgcatgc atcgggacag ggagatcaaa gtcggtccag ccgaggagaa
     1561 gcggatgggg gagtatcgcg cggcgctggt taagatcctg ccgggcagtc agccggagtt
     1621 ggagggcgca ggacggggca tgtgacgctc ggagcggtca tgttcactga agcaggatca
     1681 tccgttgaac atgccgggaa cggtcaatgt cccatctttg ggccggccgc gttggcgccg
     1741 gcggtgacca gttggaagcg gaccgtcgat ccacagtgcg gacatttcgc gcggaccgtc
     1801 tcctcgtcga ggacgtcata atgatcctgc ttcagccaca ttaattgaaa acacttcgga
     1861 cagcttcgca cttgcgttcc catggtcttc cccttacact acggttaacc gactcacccc
     1921 gttcggaggg attaatttag tacaagatgc ctcccgctct caagaccgat cagcatccca
     1981 tcacattctc cgaccgcaac gatttcgacg ctgcacaact gattcatttg taccggcagg
     2041 ctccctgggc caagcatcgt gcgcttgaac aggcgcaggc gatgctggcg aagacggatg
     2101 tagtcatttc ggcctgggat ggcactcgcc tggtgggctt cggccgcgtg ctgaccgact
     2161 acgtattccg ggcgtcgatt tgggacgtga tcgtcgatcg cgagtatcag aaacgaaaga
     2221 tcggcaccga gatcgttcga cggattctcg accatcccac ccttcagcac gttgaactgt
     2281 tttggctctg cacgcgcatg cccgggtttt atgaacggct cgggttcagc gcgaaagagc
     2341 agaccggcat ggtctggtct cggaagaagt ctgagccgca agcttcatag tccatccgcc
     2401 tccaacggtt ttcttttccg tactctccag tccctgtcga cgatccctgg tttgcctatc
     2461 cttgcctgtg aggggaacgg tcctgccggg ttgttctatg tcggctgaat tcgatgcaac
     2521 cgtttgggcg aacagcagac cgcgagaaag aaaaggctgg tggccaagag gacaatggac
     2581 ggaccggagg gaagatcaaa ggcataggag agcagtacgc cgctcacggc gcagaagatg
     2641 ccgatgagca tggagtagag cgtcatctga gtcaggctgt gcgtaagctg gtatgcggta
     2701 gatgcgggga tcagaagcat agcaaacacg agaatcgccc cgacggtttt cagcgcaatc
     2761 accacggtga gcgccacgag tgtcagcagg aggtagaaga tttgccgcgc ggggacgccg
     2821 gacgctaccg acatatcctg gtataaggcg atgaagtaaa gttccttaga gagcagaagg
     2881 atagttccca gcaccaacaa g
//
LOCUS       DAORQN010000187         2877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1103_length_2877_cov_3.857193, whole genome shotgun sequence.
ACCESSION   DAORQN010000187 DAORQN010000000
VERSION     DAORQN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_2877_cov_3.857193"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK614_08375"
     CDS             <1..203
                     /locus_tag="PK614_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB82265.1"
                     /translation="INLGVLNLLPIPILDGGHLLFFFIEAIRRKPLEDRQRELAQQVG
                     LVLLVGIMIFAFWNDIERLISP"
     gene            420..1903
                     /locus_tag="PK614_08380"
                     /pseudo
     CDS             420..1903
                     /locus_tag="PK614_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
     gene            2116..>2877
                     /locus_tag="PK614_08385"
     CDS             2116..>2877
                     /locus_tag="PK614_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB82266.1"
                     /translation="MQPKPPLPGLGDATLKSGNAENVKRISGQILAAADVDQILLDLR
                     HEILSCFDAEDLTLFVVDSEKKEIFSKIPHLDTVQEVRTPITEQSLAGFCAKYLRPVN
                     VGDAYSPVELSAIHPSLTHDASYDKNTGFKTKQVLTYPVVAENKYLMGVIQLLNKKSG
                     GRFTRKDEECVAEIAKALGTAFYALRKTSKKPPSKFDYLLTNGKVSQHDLDNALAEAK
                     KGTTDIESLLIEKYKVPKAELGKSLANFHKCPYIEY"
BASE COUNT          662 a          866 c          786 g          563 t
ORIGIN      
        1 gcatcaacct gggcgtgctg aatcttctac ccatccccat tctcgacggc gggcacctgc
       61 tgttcttttt tattgaggcc attcgtagaa aaccacttga agatcggcag cgagaactgg
      121 cacaacaggt gggactggtt ctcctggtcg gcatcatgat ttttgcgttc tggaatgata
      181 ttgagcggct gatttcccca taaacccaca tgcgcgtttc cgaaaccctt atccccacat
      241 tgcgagaaga tccaggcgag gctgaaacgg tcagccatcg cctcatgctg cgcgccggcc
      301 tgatccggaa ggtcgcggcc ggaatctaca cctatctccc gctgggcctg cgagtgctcc
      361 gtaaaatcga acgcatcgtt cgcgaagaga tgaatcgggc gggagcgcag gaactgctca
      421 tgcccgtcgc ctctccggca gagctgtggc gagaaaccgg acgttgggac ttctatggca
      481 aggagctgct ccggttcaag gaccggcacg agcgcgattt ctgcctcggg ccgacacacg
      541 aggaagtgat caccgatctc ttccgtcggg aagttcgctc ataccggcag atgccgttga
      601 atttctatca gatccagacg aaattcgtcc acgttttggt gtgatgcgct cacgcgaatt
      661 catcatgaaa gatgcgtaca gcttcgaccg ggatgaagcc ggagccagac tgaattatca
      721 aaaaatgtac gacgcataca accgcatctt cacgcgctgc ggcctcacct tccgtcccgt
      781 ggaagccgac accgggttga tcgggggcac ctcgtcacat gagttcatgg tgctggcgga
      841 gaccggcgag gagacgatcg tctatagcga agacggcaca tacgccgcca acgtcgaacg
      901 tgccgaagtc ctccctccgg ataccgctga caattcggct caccgcccgc ttacgccggt
      961 ctcaaccccg aaccgacgaa ccgtggagga agtcactgcc ttcctgaaaa ttgagccgag
     1021 acaactggtc aagacgctgc tctacagcac gggcaaagag acggtcgccg tcctggtccg
     1081 cggcgatcat gatgtgaatg agatcaaggt caaacgcctc ttgggtgtac cggagattga
     1141 gctgctgaaa cccgagttgg ttcccggcct cacgggcgcg ccggtcggat tcgttgggcc
     1201 ggtgggattg aagcaggtcc gcatcatggg cgactgggcc gtgaaggcca tggccaactt
     1261 cgtcgtcgga gccaatcagg ccgacaccca tttcgtagac gccaactggg agcgagactt
     1321 caaggtggat cagtttgccg atctccgcaa tgcacgcgcc ggtgattgtt caccccgcaa
     1381 ggacggcacg ctgaagacgg ccaagggcat cgaagtcggc catgtcttca tgttggggac
     1441 aaagtacagc caggcgatga aggccacctt cctggatgcg cagggacagg aacagctggc
     1501 ggtcatgggc tgttacggaa tcggcgtgag ccgaaccgct gcggcttccg ttgagcagaa
     1561 tcacgatgcg aaaggcatca agtggccggt tcccatcgcg cccttccatg tcaccctgtt
     1621 gccgctcagc cagtctcaac cagtcacgca gttagccgag accctctatc gctccatgca
     1681 ggaggccggt atcgaagtct tgtgggacga ccgtgacgag cgggccgggg tcaagttcaa
     1741 cgacgccgat ctgatcggcg caccctatca tttggtcatc ggggagaaag ggttgtcgca
     1801 aggtcaggtc gagctcaaac ttcgtcatac cggcgagact caaaaagtcg ctcccgacca
     1861 ggtggtctcc ctgctgtcct cattcctgaa agccgcttcc tagctccatc agccggttca
     1921 atcgttcact cccccgacag aacttcactc ctgcggacac gtccttacac gaccgacaga
     1981 atcggtttga gaaagtgtct tttacttgcc ttgactgcgc gttgcgatac actgactcac
     2041 acggaagaag cccggcgttc ggatcccggt cgacaccgca accgccctct gcgcgagcgt
     2101 tttggagaca acttgatgca gcccaaaccc ccgctgcccg gtctaggcga cgcgactctc
     2161 aaatccggca acgcggaaaa cgtcaagcgg atcagcgggc aaattctcgc cgcggccgat
     2221 gtcgaccaga tcttgctgga tctgcgccat gaaatcttga gctgtttcga cgcggaagat
     2281 ctcacgctct ttgtggtgga ctcggaaaag aaagagatct tctccaaaat tccccatctc
     2341 gataccgttc aggaagtccg caccccgatc accgaacaga gtcttgccgg gttctgcgcc
     2401 aaataccttc gcccggtcaa tgtcggcgac gcctacagtc cggtcgaact gagcgcgatc
     2461 cacccctcgc tcacccacga tgcctcctac gacaaaaaca ccggcttcaa aaccaaacag
     2521 gtgctcacct atcccgtcgt cgccgagaac aaatatctca tgggtgtcat ccagctgctc
     2581 aacaagaaaa gcggcggacg gttcacgcgc aaagacgagg aatgtgtcgc cgagatcgcc
     2641 aaggcattag gaacggcctt ttacgcgctc aggaagacgt cgaaaaagcc tccctcgaaa
     2701 ttcgactatc tgctgaccaa cggaaaagtt tcgcaacacg atttggacaa tgcgctcgcg
     2761 gaggcaaaaa aggggaccac cgacatcgag tcgctgctca tcgaaaaata taaagtaccc
     2821 aaagcggaac tcggcaagtc gctcgccaat tttcacaaat gcccctacat cgaatac
//
LOCUS       DAORQN010000188         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1104_length_2868_cov_3.937789, whole genome shotgun sequence.
ACCESSION   DAORQN010000188 DAORQN010000000
VERSION     DAORQN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1104_length_2868_cov_3.937789"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK614_08390"
     CDS             complement(<1..447)
                     /locus_tag="PK614_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRB82267.1"
                     /translation="MLHRLFAGKGLIIALAVGFIAVESIVAASFLSLLHFKTSNNWAT
                     KSEQVLLELERMMSAVAGAETSQRGYIITGSDEYLAPYREAVVTLEDQIRRIGGLTRD
                     NSIQQDRVAYLATQVEQRSEEMDQAIVTRRSKGLLYAKSAVAVNQQN"
     gene            804..2051
                     /locus_tag="PK614_08395"
     CDS             804..2051
                     /locus_tag="PK614_08395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRB82268.1"
                     /translation="MARIVIIGASIGGLPAAYEARELLAKKHKVTVISNVESFHFVPS
                     NPWVAVGWRTRKDISFALGPVLEKKGVEYIHAMAERIEPEQNRVITSKGEVPYDYLII
                     ATGPKLNFGAVPGLGPSGYTQSVCNVDHAEQAWGTYQAFLKDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKKKLRKKVPIYFVTPEPYIGHMGLAGVGASRRLMEDEFADHSMK
                     PICNTAIKEVQPGKLLLDGGQEIPFRYSMIIPPFAGVDAVAGTPGLCNPKGFVNIDAY
                     QANPKYKNIYSVGVCVAIPPVEQTPIPTGAPKTGYMIESMVSAAVHNIKADIEDDPKR
                     ETATWNAICLADMGDTGVAFVALPQMAPRNVTWAKKGKWVHLAKIALEKYFLRKMKKG
                     VSEPYYEKVILKAMGIAKLEGPA"
     assembly_gap    2072..2087
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2088..2858
                     /locus_tag="PK614_08400"
     CDS             <2088..2858
                     /locus_tag="PK614_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRB82269.1"
                     /translation="LAGGPIGLQLGATGTGGAILGLPLMVYLAGIPMQQAAAMSLIIV
                     ATSSLLGAWEYGRQGQVKAKAVLAFSWTGAIGSWAGAFGHRLVREEILLVLFGLLLLL
                     VRVAMTRHRQMSSGSDGEESCATRFPRTCWLKVGGIGLAVGTMNGLFGVGGGFMVVPA
                     LIVILGFPTRLAIGTSLSIIACISIGGVVGHLQFGRINWPLTAWVLAGSLAGMLLGVR
                     VGAWLSPSTMGRITAFITVSIAVSMIVVNLVKLWGSQI"
BASE COUNT          575 a          773 c          905 g          599 t
ORIGIN      
        1 attctgttga ttcaccgcca cggccgactt ggcatagagg agccctttgc tgcgacgtgt
       61 cacgatggcc tggtccattt cctccgatcg ctgttcgacc tgtgtggcaa gataggccac
      121 ccggtcctgt tggatcgaat tgtccctcgt cagtcctccg atccgccgga tctggtcctc
      181 aagcgtgacg actgcctcgc gatagggtgc aaggtactca tccgagccgg taatgatgta
      241 tccgcgttgg ctggtttcag cccccgccac ggcactcatc atccgttcca actcaagcag
      301 cacctgctca cttttcgtgg cccaattgtt ggaagtcttg aagtgcagca ggctcaggaa
      361 gctcgctgcg acgatggatt caaccgctat aaatcccacg gcaagggcga tgatcaggcc
      421 tttgccggca aaaagacgat ggagcatggg gaaccctcct cgataatacg tccatagtag
      481 gagtaccggc ccgtcagcgg ctatgtcaac ccgacaaatc cccctaggta tcgtaagatc
      541 tgtcgcagtg ttcgttcgag acacagtgga atgagggatc actaccccga ccccagtctt
      601 gcagcggggc ataaaatttc ccagcagccg gtttatctct actccggctg aatccaaatg
      661 acctgtgatg gctcttaaca agaggagtgg gaacggaccc gtgcatagtc ccaggtttgg
      721 agcagaacgg gtgactcgcg ggagggacgg gatcacgtag cgaggcaagg acagtaggaa
      781 tctactttga ggggaggtgg atcatggcaa ggatcgtcat catcggtgcg tccatcggcg
      841 ggttgccggc agcctatgaa gcccgggaat tgctggcgaa gaaacacaaa gtgacggtga
      901 tctccaacgt ggagtcgttc catttcgtcc cctccaatcc ctgggtggcg gtcggatggc
      961 gaacgaggaa agacatcagt tttgccctgg gccctgtgct ggagaaaaaa ggcgtggaat
     1021 acattcatgc catggccgag cggattgagc ccgaacagaa tcgtgtcatc acctcgaagg
     1081 gcgaagtccc ctatgactac ctgattatcg ccaccgggcc gaagctgaac ttcggcgcgg
     1141 tgcccggact cgggccaagc gggtatacgc aatcggtctg caatgtggac catgccgagc
     1201 aggcctgggg cacctaccaa gcgttcctga aagaccccgg tcccatcgtg gtaggggcgg
     1261 cgcaaggcgc ctcctgtttc ggaccggcct acgaaatggc cttcattctg gatgccgatc
     1321 tccgcaagaa gaaactcagg aagaaggtgc cgatctattt tgtgacaccg gaaccctata
     1381 tcggacatat ggggctcgca ggtgtgggcg cctcccgccg tttgatggaa gatgagtttg
     1441 ccgatcattc gatgaagccc atctgtaata cggcgatcaa agaagtgcag ccgggcaaac
     1501 tcttgctcga cggagggcag gagatcccgt tccgctactc gatgatcatt ccgccgttcg
     1561 ccggcgtgga tgcggtggca gggacgccgg ggctctgcaa ccccaaaggg tttgtgaata
     1621 tcgatgccta tcaagccaat cccaagtata agaatatcta ttcggtcggc gtctgtgtgg
     1681 ccattccgcc ggtggagcag actccgattc cgaccggtgc gcccaagacc gggtacatga
     1741 tcgagtcgat ggtgtcggcg gcggtgcaca acatcaaggc cgatatcgag gatgacccga
     1801 agcgcgagac cgcgacctgg aatgcgatct gtctcgccga catgggggat acgggcgtgg
     1861 cctttgtggc gttgccgcaa atggcgccgc ggaatgtcac ctgggcgaag aagggcaagt
     1921 gggtacacct tgccaaaatt gcgctggaga agtatttctt gcgcaagatg aaaaaaggcg
     1981 tcagcgagcc ctattacgag aaggtgattt tgaaggccat ggggattgcc aagttggaag
     2041 gaccggccta aacgacatgg ggacggggca tnnnnnnnnn nnnnnnnctg gcgggcggcc
     2101 ccatcggctt gcaactcggc gcgacgggga cgggtggcgc gattctgggc ctccccttga
     2161 tggtctacct cgccgggatc cccatgcagc aggcggcggc gatgtcgctg atcattgtgg
     2221 caacctcgtc gttgctgggc gcgtgggaat atgggcggca gggacaggtc aaggccaaag
     2281 ccgtcctggc gttcagttgg accggcgcga tcggttcgtg ggcgggcgcg ttcgggcatc
     2341 gcctggttcg cgaggaaatc ctgctggtgc tgttcggact cctgctgctg ctggtcagag
     2401 tggcgatgac tcgccatcgt cagatgtctt ccggatctga cggggaagaa agttgtgcga
     2461 cccgctttcc ccgtacctgc tggctgaagg tcggcggcat cgggctggcg gtgggaacca
     2521 tgaacggctt gttcggcgtc ggtggcggat tcatggttgt cccggccctg atcgtcatcc
     2581 tcggattccc gaccaggctg gcgatcggga cgtctctcag catcatcgcc tgtatctcca
     2641 tcggcggggt ggtcggccat ctccaattcg ggcggatcaa ctggccgctg acggcgtggg
     2701 tattggcagg aagtctggcg gggatgcttc tgggggtgcg ggtcggcgca tggctttctc
     2761 cctccacgat ggggcgcatc acggccttca tcacggtttc gattgcagtc agcatgatcg
     2821 tggtgaatct ggtgaagtta tggggttcac aaatataaac tttattga
//
LOCUS       DAORQN010000189         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1107_length_2861_cov_7.631860, whole genome shotgun sequence.
ACCESSION   DAORQN010000189 DAORQN010000000
VERSION     DAORQN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1107_length_2861_cov_7.631860"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="PK614_08405"
     CDS             <1..1618
                     /locus_tag="PK614_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRB82270.1"
                     /translation="LVGLGGRTIQDLYVPMADAPRTFGFPPGSSRLFIEAIVIGSGVS
                     ILGALGPSLDAGRTMVVAALAPGEYDVAQRVRAASLGVVGGGLLLFALGCALAGPVGG
                     VPVFGYLATFCLLAGLSCLVPILMQWVCRTRELGAASASPSLGGAIRHIAREQTTRGI
                     GRNAVTVSAFLVGVAIMVGVMVMIRSFRDTVEMWIDQTVMADFIVAPAGWPHVVRGGS
                     SSQALPGSWRTRLAGATHVSAVDAYRDVRIELEGRPIALVSRDLALHAARSRYLFLEG
                     DSAAILTRAAAGEGAILSEVVANHLHVIRGSQLSISTPVGEKSLPVLGVFYDYATDGG
                     KIVIDRSLYQHWWNDDGVTVFPVYIDPGVDLEQARAALLETLADGSRGSLLPTVLSNG
                     ELRREILRIFDRTFTLTYVLEAIAIIIAMLGIINTLVTSVVERRRELATLQALGSSRG
                     QITALILWEAGYLGLLGTAMGLCGGLALAWILIRVINRQSFGWTIQVSWPLGLMAEVA
                     VLALVASLLAGFWPARWAGRQPLVEGLRYE"
     gene            complement(1609..2640)
                     /gene="bioB"
                     /locus_tag="PK614_08410"
     CDS             complement(1609..2640)
                     /gene="bioB"
                     /locus_tag="PK614_08410"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRB82271.1"
                     /translation="MTDFTILADKALRDEILTHEESLAVLQTPDSRLLELLQAAFTVR
                     ERYFGKTVRLQMLLNAKSGACQEDCGYCSQSAISKAPIERYGLLTQDQMVTGARRAAA
                     AKAQRYCIVISGRSPIDRDIADIASAVRSIKQEVPIQICCSLGLLNERQAKDLKAAGV
                     DRINHNLNTSEAYHPEICTTHTFQDRLATIRHARTAGLEICSGGIVGMGESDEDLIDL
                     ALALREVKPDSIPINTLHPASGTPMEHCAPLTPQRCLKALCLFRLLHPRTEIRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGQPAAEVWRMIEDLGFEIQVDAVPTKQGVPA
                     SEPVAGLHS"
BASE COUNT          458 a          775 c          994 g          634 t
ORIGIN      
        1 actcgtcggg ttgggcggtc ggaccattca ggacctctat gttccgatgg ctgatgcgcc
       61 aagaaccttc ggctttccgc cgggatcgag ccggctgttc atcgaagcga ttgtgatcgg
      121 tagcggggtg tcaatcctgg gagcgctggg gcccagcttg gatgccggac gtaccatggt
      181 ggtcgcggcc cttgctcctg gggaatatga tgttgcccag cgagttcgtg ctgcctcact
      241 gggggtggtg ggtggaggcc tgctgctctt cgcgctggga tgcgcgctag ccggaccagt
      301 cggcggggtt ccggtcttcg ggtatctggc gacgttctgt ctgcttgccg gtctctcctg
      361 cctggttccg atcctcatgc agtgggtttg ccgtacccga gagttgggcg ccgcttccgc
      421 ttcgccttcg ttggggggcg ccattcgcca cattgcgcgg gagcagacga cgcggggaat
      481 cggccggaat gcagtgaccg tctctgcctt tctcgtcggg gtcgcgatta tggtcggggt
      541 aatggtgatg attcggagtt ttcgcgacac ggtcgagatg tggatcgatc agacggtcat
      601 ggccgatttc atcgtggctc cggccgggtg gccgcatgtg gttcgcggtg ggtcgtcgag
      661 ccaggccctc cctggttcct ggcgaacccg tctggccggg gccacgcatg tgtcggcggt
      721 cgatgcgtac cgcgatgtac gcatcgaact ggaggggcgg ccgatcgcgt tggtatccag
      781 agatctggcg ctccatgcgg cacgaagccg atatctattt ctcgagggtg actcggcggc
      841 aatcctgact cgtgccgcag caggagaagg ggctatcctc tcggaggtgg tggccaatca
      901 cctgcatgtg atcaggggca gtcagttgtc gatttcgacg ccggtggggg aaaaatcgtt
      961 gccggttctg ggggtctttt acgactacgc gaccgatggc gggaagatcg tcatcgaccg
     1021 gtctctgtat cagcactggt ggaacgatga cggcgtgacg gtgtttcctg tgtatatcga
     1081 tccgggagtc gatctggaac aggccagagc cgccctgctt gaaaccttgg ccgatggctc
     1141 ccgcgggagt ctgttgccga cggtgttaag caacggagaa ttgcgccggg agatccttcg
     1201 aattttcgac cggaccttca cattgaccta tgtgctggag gccatcgcga tcattatcgc
     1261 catgctgggg atcatcaaca ccttggtgac gtcggtggtc gagcggcgtc gggagttggc
     1321 cacgttgcag gcgctgggca gcagccgggg acagattacg gctctgattc tctgggaagc
     1381 cggctatctg ggcctgttgg gaacggcgat gggactgtgc ggcgggcttg cgctcgcctg
     1441 gattttaatc cgggtcatca accggcagtc gttcggctgg acgatccagg tttcgtggcc
     1501 cctggggttg atggcggaag tggcggtgct ggctctggtc gcttcgctcc tcgccgggtt
     1561 ttggccggct cgttgggccg gccggcaacc gctggttgag gggctccgtt acgaatgaag
     1621 gcccgcgact ggctctgatg caggaactcc ctgcttggtc gggactgcat ccacctggat
     1681 ttcgaatccc aggtcttcaa tcattcgcca cacctctgcc gccggttgac ccggcgtggt
     1741 gaggtatccg tttacgaaca cggaatccgc cggatagagc gcgagcggct gcaagctgcg
     1801 cagattgtgt tcgcgacctc cggcgatacg gatctccgtt cgcgggtgca ggagccggaa
     1861 gaggcacagg gccttcaggc accgctgcgg cgtcaggggg gcgcaatgct ccattggagt
     1921 accggatgcc gggtgtagcg tgttgatggg aatcgagtct ggtttgacct cacggagggc
     1981 cagggcgaga tcgatcaggt cttcatcgct ctctcccatt ccgacgatgc caccggaaca
     2041 gatttcgagt ccggccgtcc gcgcgtgccg gatggtggcc agccggtcct ggaaggtgtg
     2101 tgtcgtgcag atctcggggt gataggcttc gctcgtgttc aggttgtgat tgattcggtc
     2161 caccccggcg gctttcaggt ctttcgcctg gcgttcgttg aggagcccca gcgagcagca
     2221 gatctgaatc ggaacttcct gcttgatgga gcggacggcg gaggcgatat cggcaatgtc
     2281 ccgatcgatc gggctgcgtc cgctgatgac gatacaatat cgctgggctt ttgcagccgc
     2341 cgcgcggcgg gcgccggtga ccatctggtc ttgggtgagc agtccgtagc gttcgatcgg
     2401 tgctttggag atggccgatt gcgaacagta gccgcagtct tcctggcacg cgccgctctt
     2461 ggcgttcaag agcatttgca ggcgaaccgt cttgccgaag tagcgttcac ggacggtgaa
     2521 agccgcctgc aacagttcca gcagtcggga gtccggtgtc tgcaatacgg cgagggattc
     2581 ctcgtgggtg agtatttcgt cccgcaaggc cttgtcggcc aggatcgtga agtcagtcat
     2641 gcacgctact cctcgaaaag acagatcgaa ccttgattcc cgatcgggag acccgtgggc
     2701 ggaggccgtg tctgtgaaaa gaaaaaaggg cggagaaacc ctacacggtt tcaaatggag
     2761 atgcaatgcc ttgataaggg acattcggga tgcgcgaagc ctggccgtcc tggtcggtat
     2821 cgggctggtt cgcgtcccgc cagggaaggg cgacgtaggt g
//
LOCUS       DAORQN010000190         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1110_length_2856_cov_5.121742, whole genome shotgun sequence.
ACCESSION   DAORQN010000190 DAORQN010000000
VERSION     DAORQN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_2856_cov_5.121742"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..747)
                     /locus_tag="PK614_08415"
     CDS             complement(88..747)
                     /locus_tag="PK614_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB82272.1"
                     /translation="MTTESTSRPLNVNSLTPRQIVEALDRYVIGQHDAKRMVAIALRN
                     RWRRQQLAPELRDEVMPKNIIMIGPTGVGKTEIARRLAKLAEAPFIKVEASKFTEVGY
                     VGRDVESIIRDLTELAISLVKTKHLEEVQGKASRHGEERLLDLLLPGAPFRPASPGFE
                     PSGARADTAEPTESPDATRSKLRLQLREGKLDQRSVEVEGQGAGIAAGGDFQCRRHGG
                     P"
     gene            complement(760..1296)
                     /gene="hslV"
                     /locus_tag="PK614_08420"
     CDS             complement(760..1296)
                     /gene="hslV"
                     /locus_tag="PK614_08420"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRB82273.1"
                     /translation="MMIRSTTVLCVRRGSQVTMGCDGQVTVGTTVMKHNARKMRRMHS
                     DTVLAGFAGATADAFTLFEKFEAKLAEYRGNLTRAAVELAKDWRTDRVLRRLEALLAV
                     ADLDHSFIISGTGDVVEPEDGILAIGSGGPYALAAARALLGHSELVAEQVVRESLMIA
                     GGIDIYTNQQIVIESLSR"
     gene            complement(1293..2246)
                     /locus_tag="PK614_08425"
     CDS             complement(1293..2246)
                     /locus_tag="PK614_08425"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRB82274.1"
                     /translation="MDRAIRTFLDVLSVQQSASPQTIRAYASDLAQFHAFALGVLKPG
                     GPLAPHAVAPALIREFLAARDRKGEKKTSLARKLACLRSFFRYLVRIGQLEVNPAEDV
                     RAPKLPKHLPQVLTKDDAGALMEFPGGTEREGVRDRAILETLYSTGARVSELVGMNCD
                     DISRSEGLVRVRGKGRKERVIPLGSIALEAIDAYHAQIQMVPGLSSGRSDAVAVFRNS
                     RGGRLTTRTIARIVAKYSRQLTGGAVHPHTLRHSFATHLLDEGADLRAIQEMLGHASL
                     STTQKYTHLATDQLLALYDRTHPRAVRTTEASESTKQKGSR"
     gene            complement(2219..>2856)
                     /gene="trmFO"
                     /locus_tag="PK614_08430"
     CDS             complement(2219..>2856)
                     /gene="trmFO"
                     /locus_tag="PK614_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRB82275.1"
                     /translation="PIEVMAERGRQTMQFGPLKPVGLEHPKTGVRPYAVVQLRTENAH
                     GTCYNMVGFQTKLTYPEQRRVFRLIPGLENAEFLRLGSLHRNTFINSPQLLRETLQLK
                     SRGTVFFAGQLVGVEGYTESAAMGGIAGINAARGLADQPLVTPPPNSAHGCLIAHITK
                     SDPAHFQPMNTNFGLFPPVTVKTRDKDQKRRLIQQRAVEDFDAWIAQSGLS"
BASE COUNT          557 a          903 c          819 g          577 t
ORIGIN      
        1 agatgtttca gcgcatcagg cactttcatc atccgtttct ttttcttccc ttggaacatg
       61 ccgcccagca tgtcgcgcag attcccttca aggtcctcca tgccgccggc attggaaatc
      121 acccccagcg gcaatgcccg ctccttgacc ctctacttcc acagatcgct ggtcgagctt
      181 gccctcgcgc agttgcaagc gaagcttgga tcgcgtggca tccggcgatt cagtcggctc
      241 tgcagtatcg gcacgcgccc cggagggctc aaatccaggg gaagctggac ggaacggtgc
      301 accgggcaac aggagatcaa gcagccgctc ttctccatgg cgcgacgcct tcccctgcac
      361 ctcttccaag tgtttggtct tcactaaact gatggccagc tcggtgagat cacgaatgat
      421 ggattcgaca tcccgtccga catagccgac ttccgtaaac ttcgaggcct ccaccttgat
      481 gaagggcgct tcggcaagtt tcgccaggcg tctggcaatt tcggttttgc ccacgccggt
      541 cggcccgatc atgatgatgt tcttcggcat aacctcatca cgcaactcag gagccaactg
      601 ctgacggcgc cagcggttgc ggagcgcaat cgcgaccatg cgcttggcat cgtgctggcc
      661 gatcacatag cggtccagcg cttcgacgat ttgccggggc gtcagactat tcacattgag
      721 cgggcgggac gtcgattcgg tcgtcatggc aggcagatcc taacgcgaga gcgattcgat
      781 cacaatctgt tggttggtat aaatgtcgat cccgccggca atcatcaggg attcccgcac
      841 gacctgttcg gcaaccaatt cggaatgccc tagcaaggcc cgggcggcgg ctaatgcata
      901 ggggccaccc gatccgatcg ccaggatgcc gtcttccggc tcaacgacat ctccggtccc
      961 ggagatgatg aaggagtgat cgagatcggc aacggccagc aacgcctcca agcgccgtaa
     1021 cacgcgatca gtccgccagt ccttcgccag ttccacggcg gctctagtga gatttccgcg
     1081 atactcggcc aacttggcct caaacttttc aaacagggtg aaggcatcgg ccgtggcgcc
     1141 agcgaacccc gccagcaccg tatcgctgtg catacggcgc attttcctgg cattgtgctt
     1201 catgaccgtg gtgccgaccg tgacctgtcc atcacagccc atcgtcacct ggctgccccg
     1261 tcgcacacac aacaccgtgg tggatcgaat catcatcgcg accccttctg cttggtggac
     1321 tcgctcgcct ccgtggtacg cacggcacga gggtgagtgc gatcgtataa cgcgagtagt
     1381 tggtccgtcg caagatgtgt atatttttgt gtcgtactga gcgaggcatg tcccagcatt
     1441 tcctgaatgg cacgtaggtc ggcaccttca tccagcaaat gcgtggcgaa cgaatggcgc
     1501 aaggtgtggg gatggaccgc gcctccggtc agttgccgcg aatatttggc cacaatgcgc
     1561 gcaatggttc tggtggtcaa tcggcccccg cggctattgc gaaagacggc cacggcgtcc
     1621 gatcgccctg aggacagtcc cggcaccatc tggatctgcg catggtaggc atcgatcgcc
     1681 tccagcgcaa tgctgcccag cggaatgacc cgttctttcc gccccttccc gcgcacgcgc
     1741 accagtcctt cgctccggct gatatcgtcg caattcatgc cgaccaactc gctgacgcga
     1801 gcccccgtgg aatacaaggt ctcgaggatc gcacgatccc gtaccccttc ccgctccgtt
     1861 ccaccgggga actccatcaa cgcaccggca tcgtctttcg tgaggacctg gggaaggtgc
     1921 ttgggaagtt tcggggcgcg cacatcctct gcggggttca cttccaactg cccgatgcgc
     1981 accagatacc ggaagaaact gcgcagacag gccagctttc tcgccaacga ggtcttcttt
     2041 tcccccttgc gatcacgggc ggcgagaaat tcgcgaatca gagccggtgc caccgcgtga
     2101 ggagccagcg gcccgcccgg cttcagcacc cccagcgcga aggcgtggaa ctgagccagg
     2161 tcggacgcat aggcacgaat agtttggggg gacgcgctct gctggacaga aaggacgtct
     2221 aggaaagtcc ggattgcgcg atccatgcgt caaaatcctc aacagctcgt tgctggatga
     2281 gacgccgctt ctgatccttg tcgcgcgtct tgacggtgac gggcgggaac agcccgaagt
     2341 tcgtattcat cggctgaaaa tgggccggat cgctcttggt aatatgcgca attaaacagc
     2401 cgtgggcgct gttcggcggc ggagtcacca acggctggtc cgccaggcct cgcgccgcat
     2461 tgatgcctgc aatccctccc atcgcggccg actcggtgta cccttcgacc ccgaccaatt
     2521 gtccggcaaa aaatacggtg ccacgggact tgagctggag cgtctcgcgc agcaattgcg
     2581 gcgagttgat gaaggtgttg cgatggagac tgccgagccg tagaaattcg gcattctcca
     2641 gccccggaat gaggcgaaac acccggcgct gctccggata cgtcagtttg gtctgaaacc
     2701 cgaccatgtt gtaacaagtc ccatgcgcgt tctcggtacg aagctgcacc acagcgtagg
     2761 ggcgcacgcc tgtcttcgga tgctccagcc cgaccggctt gagcggtccg aactgcatgg
     2821 tctgccgacc acgctcggcc atgacttcaa tcggga
//
LOCUS       DAORQN010000191         2855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1111_length_2855_cov_9.045714, whole genome shotgun sequence.
ACCESSION   DAORQN010000191 DAORQN010000000
VERSION     DAORQN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_2855_cov_9.045714"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /gene="rsmG"
                     /locus_tag="PK614_08435"
     CDS             complement(<1..509)
                     /gene="rsmG"
                     /locus_tag="PK614_08435"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRB82276.1"
                     /translation="MEHNELQFEKSLQSLAAEFSISIHDSSVSYFFRYLSELKKWNRA
                     INLTAIDDDREILVKHFIDSIYGLKVIDINREIELLDVGAGAGFPALPLKFVRPQLSV
                     ELLEPSEKKGAFLRYIVGSLGLQNVTVATAKLEDYAGRGNVQGRFDYIVVRAFKVDQF
                     GVALSTLLKP"
     gene            complement(613..2487)
                     /gene="mnmG"
                     /locus_tag="PK614_08440"
     CDS             complement(613..2487)
                     /gene="mnmG"
                     /locus_tag="PK614_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRB82277.1"
                     /translation="MSEQCDVIVVGGGHAGCEAALAAARMGARTILLTMDPDRIAQMS
                     CNPAIGGIAKGHLVKEIDALGGEMGRNTDQAGIQFRMINTSKGPAVRALRAQCDKKVY
                     REVMQRTLRAQPALEIRGGTVDRILTRGRAVTGIVTDAGTSIHARAVVLTSGTFLKGL
                     IHIGLNHFPAGRAGEASAEHLSDCMRDFGFEVGRLKTGTPPRLDRDSIDFSVMELQPG
                     DDPPPPFSYRTLRVSLPQVPCHLTYTNSQTHDIIAKNIDRSPLFSGVIGSVGPRYCPS
                     IEDKVVRFADKDRHQIFIEPEGLDTNEFYPNGISTSLPVDVQAAILKTIPGLEQARML
                     KPGYAVEYDYFPPRQLHNSLETKLVAGLYHAGQINGTSGYEEAGAQGIMAGINAALRW
                     RGEEPLVLDRSQAYIGVLIDDLITKDAREPYRMFTSRAEYRLLLRHDNADLRLMDLGH
                     RIGLVSDEVHGKLEAKRSAIEREVQRLKETRPKITSDVKRLMEEAKIGTVAPTQSLAE
                     ILRRQEMTYEKIVAVFGGANIDDLDIAEAIQLEVKYEGYIKRQLQQIQRSEKLEHRAI
                     PDQFDYDGITGFSGEVREKLKRVKPASIGQASRISGVTPAAISLLLVAIERHRRQPSA
                     "
     gene            complement(2501..>2855)
                     /locus_tag="PK614_08445"
     CDS             complement(2501..>2855)
                     /locus_tag="PK614_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82278.1"
                     /translation="SKEDLIRVCPVNAGVLDISAKMQIGLDGLRDAISSRLMPRGLES
                     REGVLVTNLRHAAAFERALQGVEQARQSVEAGMAGELVAMDLRIAADALGEITGAITT
                     DEILERIFAEFCIGK"
BASE COUNT          686 a          863 c          678 g          628 t
ORIGIN      
        1 ggcttcagaa gcgtcgacag cgccacgccg aactgatcca ccttaaaggc cctcacgacg
       61 atgtaatcaa accgcccctg aacatttcct cgaccggcat aatcttccaa cttggccgtc
      121 gcaaccgtca cgttctgcag accaagcgat cccacgatat accgcaaaaa cgcccccttc
      181 ttttcgctcg gctcaagcaa ttcaacggac agctgaggcc gtacaaattt gaggggcaga
      241 gccggaaatc cagcaccggc accaacatcg agcaactcaa tttcacgatt aatatcaatg
      301 actttaagcc catagattga gtcaataaaa tgcttcacca ggatctctcg atcatcgtcg
      361 atggccgtga gattgatggc cctattccat tttttcagct cgctgaggta acgaaaaaaa
      421 taagaaacag aggaatcatg aatggaaatg gagaactcag cggcaagaga ctgaagagat
      481 ttttcgaact gtagctcgtt gtgttccacg tggaacactt acgcgaaaag aaccaacagg
      541 gtcaagcaag ttcttcctta tcatgggcaa agtgttagcg agaatccatt cccggcaatc
      601 cagcacacca tttcatgccg atggttgccg acgatgcctc tcgattgcaa ccaacagaag
      661 ggaaatagcg gcaggcgtca caccggaaat acgcgaagcc tgccctatcg atgccggctt
      721 cacacgcttg agcttctccc tgacttcacc tgagaaaccg gttattccgt cataatcaaa
      781 ctgatctgga atcgctctgt gctctagctt ctccgatcgc tgaatctgct gcagttgccg
      841 cttaatatat ccttcgtatt tcacttcgag ttgaatcgct tccgcgatat caagatcgtc
      901 aatattagcg ccaccgaaca ctgcgacgat cttttcatac gtcatttcct gacgcctgag
      961 aatctcagcc aacgattgcg ttggtgccac agtgcctatc ttcgcctctt ccatgagccg
     1021 cttcacatca gacgttattt tcggcctggt ttccttgagg cgctgaactt cccgttcaat
     1081 agcagaccgc ttcgcctcca gcttcccgtg cacctcatca gataccaatc cgatgcggtg
     1141 cccgagatcc atcaggcgaa gatcggcgtt gtcatggcgg agcaacagac ggtattccgc
     1201 gcgcgaggtg aacatccgat agggttctct ggcatctttc gtaattaaat catcaattaa
     1261 cacgccaata taggcctgcg accgatccag cacgagcggc tcttcaccac gccacctcaa
     1321 ggccgcgttt attccagcca taattccctg ggcaccggcc tcttcatacc cggacgttcc
     1381 attgatttgg ccggcgtggt agagcccggc aactaatttc gtctctagcg aattgtgaag
     1441 ttggcgggga ggaaaataat cgtactcgac cgcataccca ggcttcagca ttctcgcctg
     1501 ctcaagtccg ggaatagttt tcaggatggc cgcttgcaca tcgaccggga gactggttga
     1561 aattccattc ggataaaact cgttcgtatc caacccttcc ggctcgatga agatctgatg
     1621 ccggtctttg tcggcaaacc tcacgacctt atcctcgatc gatgggcaat atcggggacc
     1681 gacagagcca atcaccccgc tgaacaacgg cgatctatca atatttttcg caataatatc
     1741 atgcgtttgc gagttcgtgt acgtcaaatg gcagggcacc tgtggcaagg acacacgcag
     1801 cgttcggtaa gaaaacggcg gtggaggatc atctcctggc tgcagctcca tcacagaaaa
     1861 atcgatagaa tctcgatcga ggcggggtgg agttcccgtc ttcaaccgac caacctcaaa
     1921 tccaaaatca cgcatgcaat cggagaggtg ctccgcggac gcctccccag cacgaccagc
     1981 gggaaaatga ttgagaccga tatggatcaa ccccttcaaa aatgtgcccg acgtaagcac
     2041 tactgcccgc gcatgaatag acgtaccggc atccgtgacg ataccggtca cagccctacc
     2101 ccgggtgaga atgcgatcaa ctgtcccgcc cctgatctca agtgccggct gcgcacggag
     2161 cgtgcgctgc atgacttcgc gatagacctt cttatcgcat tgcgcgcgca aggcacggac
     2221 cgccggaccc ttgctggtgt tgatcatccg aaactggatg ccggcctggt cggtattccg
     2281 ccccatttct ccgcctaacg catcgatctc cttgacgaga tgtcccttgg caatgccgcc
     2341 gatcgcaggg ttgcaggaca tctgagcgat ccggtccgga tccatcgtga gcaggatcgt
     2401 gcgcgccccc atgcgggctg cggccagagc cgcttcacac ccggcatggc ctcccccgac
     2461 gacgatgacg tcacactgtt cactcacgac aagagtcctt tcatttcccg atgcaaaact
     2521 cagcgaaaat gcgctccaga atttcgtcgg tcgtgatagc cccggtaatt tcacccaggg
     2581 cgtcggcagc gatacgaaga tccatcgcaa ctaattctcc cgccatccct gcctcgaccg
     2641 actgtctggc ctgctccacc ccctgcaacg cgcgctcgaa agcagcggca tgccgaagat
     2701 tggtcacaag gaccccttcc cgggactcta aacccctcgg catcaatcgg ctgctgatcg
     2761 catctcgcaa tccatccagt ccaatctgca ttttcgcaga gatatcgagc acaccggcgt
     2821 tcacgggaca gacgcggata aggtcttctt tcgag
//
LOCUS       DAORQN010000192         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1130_length_2777_cov_9.360764, whole genome shotgun sequence.
ACCESSION   DAORQN010000192 DAORQN010000000
VERSION     DAORQN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1130_length_2777_cov_9.360764"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK614_08450"
     CDS             <1..440
                     /locus_tag="PK614_08450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRB82279.1"
                     /translation="AKPLWIFYFLQKNLCAALSVYDHAAGIQHVFEQGEPGTVYNVGG
                     GNERENITVAEQIVATLGKSRSLIRFVQDRPGHDRRYSIDCRRLRALGWSPQVSFEEG
                     LKQTVEWYRTHESWWRTIKSGEFKDYYQQQYAKRLQQGTACGS"
     gene            428..1270
                     /gene="rfbD"
                     /locus_tag="PK614_08455"
     CDS             428..1270
                     /gene="rfbD"
                     /locus_tag="PK614_08455"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRB82280.1"
                     /translation="MRIVITGAQGQLGTDLRLVLQEHQLTLLDLPTFDLTHQDCGRVI
                     VEAVPEVVIHAGAHTDVDGAERNPALALAVNAEGTERVARAAALVGARLIYISTDYVF
                     DGRGTRPYVESDPTNPASAYGESKRAGEQRALACCENTLVVRTAWLYGLHGRNFVKTM
                     LQLAAERPSLKVVADQRGCPTFSADLADMIGKLVAHPAQGVLHVTNAGDCTWHEFASE
                     IVRLSGHRVPVEPITTADMPRPAKRPAYSVLSAERLHHLGFTMPSWQDGLARFLSVQS
                     KLAS"
     gene            1316..1945
                     /locus_tag="PK614_08460"
     CDS             1316..1945
                     /locus_tag="PK614_08460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HRB82281.1"
                     /translation="MKRGRMKWWSKLLVAFGIRSGGPSPLFMQHNLRYAAFEVGEGTY
                     GHPDIVVPDDGSTVRIGRYCSIAGGVSILTSQEHHLDWVSTFPFQVIFGNDRGLPRPS
                     RSKGDVSIGHDVWIGTEALILSGVSVGHGAVVAARSVVTKDVPPYAIVAGVPARVIRH
                     RFDPATIASLLRIAWWNWPREKIEAALPLLMSDRLDAFLAQYDAVSDQT"
     gene            2362..>2777
                     /locus_tag="PK614_08465"
     CDS             2362..>2777
                     /locus_tag="PK614_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82282.1"
                     /translation="MTVATSSPVDPTIRSVQEGEQHASARAFFWGFQSYVMIAIACSS
                     FVPVLFRYQEHTVIILIAFCLGMCALEKKSPWIKNPLDLPLWLFMIWVLCTVPFATDS
                     AYSFAEWKKFVAQAGVFYWSLLVLDRCRREHLPQQIL"
BASE COUNT          499 a          758 c          866 g          654 t
ORIGIN      
        1 acgccaaacc gttgtggatc ttttacttcc ttcagaagaa tctttgcgcc gcgttgtcgg
       61 tctacgacca tgcggcaggg attcagcatg tgttcgaaca gggggagcct gggaccgtct
      121 acaatgtcgg gggcggcaat gagcgggaga atatcacggt ggccgaacag atcgtggcga
      181 cgctggggaa atcccgctcg ttgattcgtt ttgtgcagga ccgcccgggg cacgatcgtc
      241 gctattcgat cgattgtcgc cgcctgcgcg ccttgggttg gtcgccgcaa gtgtccttcg
      301 aggaggggct gaagcagacc gtcgagtggt atcgcaccca tgagagctgg tggcggacga
      361 tcaagtccgg agagttcaaa gactattatc aacagcagta cgcgaaacgg ttgcagcagg
      421 ggacggcgtg cggatcgtaa tcaccggggc gcagggacag ttgggcacgg atctccggct
      481 ggtcttgcag gagcatcaac tgacgttgct cgatctcccg acgttcgatc tgacgcatca
      541 ggactgcggc cgtgtgattg tcgaggccgt gcccgaagtc gtgattcacg ccggggctca
      601 tacggatgtg gacggagcag aacggaatcc ggccctggca ctggccgtga acgccgaggg
      661 gaccgagcga gtggcccgtg cggccgcctt ggtcggagcg aggcttatct atatttccac
      721 cgactatgtg ttcgacggcc ggggcacacg tccctatgtc gaatccgatc cgaccaatcc
      781 cgccagcgcc tatggcgagt cgaaacgggc gggtgagcag cgggccttgg cctgttgtga
      841 gaataccttg gtggtgcgca ccgcctggct ctatggcctg cacggccgga atttcgtcaa
      901 gaccatgttg caactggcgg ccgaacggcc ctcgttgaaa gtcgtggccg accagcgggg
      961 ttgtccgacc ttttccgcgg atcttgcgga catgatcgga aaactcgtgg cgcatccggc
     1021 gcagggtgtg ttgcacgtga cgaacgcggg ggactgcacc tggcatgagt ttgcctcgga
     1081 gattgttcga ttgtccgggc accgggtccc cgtcgaaccg attacgacgg ccgatatgcc
     1141 gagaccggcg aagcgaccgg cctattccgt attatccgca gagcgtctcc atcatctcgg
     1201 gttcaccatg ccatcctggc aggatgggtt ggcgcgtttc ttgagcgtac agagcaagct
     1261 cgcctcctag cgcagtcggg ttcagcgggt ctctgcatcc ggagagcgag cagctgtgaa
     1321 acgggggcgt atgaagtggt ggtcaaaact gctggtcgcg ttcggtatca ggagtggtgg
     1381 gccttctccc ctattcatgc agcataatct tagatatgct gccttcgagg tgggggaggg
     1441 aacctacgga catccggata ttgtcgtgcc tgacgacggc agcacggttc gcattggacg
     1501 gtattgttcc atcgccggtg gggtctcgat tctcaccagc caagagcatc acctcgattg
     1561 ggtctcgacc tttccgtttc aagtcatctt tggaaacgac cgaggtcttc ccagaccctc
     1621 tcgttcgaaa ggcgatgtga gtatcggcca cgatgtgtgg atcgggaccg aagccttgat
     1681 tctctcagga gtctctgtgg ggcatggagc ggtggtcgcg gcccggagtg tggtcacgaa
     1741 ggatgtcccc ccctatgcca ttgtggcggg tgtgccggca cgcgtgatcc ggcaccggtt
     1801 cgatccagcc accattgcca gtttgctccg catcgcctgg tggaattggc cgcgtgaaaa
     1861 aattgaagca gcccttcctc tgttaatgtc agatcggttg gatgccttcc tcgcacagta
     1921 cgatgcggtc agtgatcaga catagagcct ctcactcggc ctcgctttcg gatcggtccc
     1981 gctgaacgcc tccccttggt gcctgacgct tcctcgcctt tcacgtcctc tccgctcagg
     2041 gtactcgttg cctggatgag acatggctcg agcgccgtgt ttgttgtaga aggggagaaa
     2101 tttctctata ctgcgcttcc acagagcctc cagcacgtcc ctgcgtgttg tgctggggtg
     2161 cgccgatggt gagtgtgacc ccatcatgta gctgcctcct cagacaggtg aataggtgat
     2221 ccgtattcca tgggatggac tgcatgtcgg gatgatctgt ccacgtccgg gggaggcatg
     2281 gtcatgcgtc atctgcgtgt gacgccgtgg ccggagtatg attgctcgag ttgatttgcg
     2341 tagaacgtgg tctgtgacgt tatgactgtt gcgacttcat cacccgtcga cccaactatc
     2401 cgatccgttc aagaaggaga gcagcatgcc tctgctcgtg cctttttttg gggatttcaa
     2461 agttatgtga tgatcgccat cgcctgttcg agtttcgttc ccgtgttgtt tcgttaccaa
     2521 gaacataccg tcattatcct cattgcgttt tgcctgggca tgtgtgcgct ggaaaagaag
     2581 agcccgtgga tcaagaatcc gctggatctt cccctgtggc tcttcatgat ctgggtgctc
     2641 tgcaccgttc ccttcgcgac tgactccgca tacagttttg ccgaatggaa aaaattcgta
     2701 gcgcaggccg gggtctttta ttggtccttg ctcgtgttgg atcgctgtcg acgggagcac
     2761 ttgccgcagc agattct
//
LOCUS       DAORQN010000193         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1137_length_2751_cov_5.818249, whole genome shotgun sequence.
ACCESSION   DAORQN010000193 DAORQN010000000
VERSION     DAORQN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_2751_cov_5.818249"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..687
                     /gene="rpoD"
                     /locus_tag="PK614_08470"
                     /pseudo
     CDS             1..687
                     /gene="rpoD"
                     /locus_tag="PK614_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            838..914
                     /locus_tag="PK614_08475"
     tRNA            838..914
                     /locus_tag="PK614_08475"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ile,seq:cat)
     gene            992..1768
                     /locus_tag="PK614_08480"
     CDS             992..1768
                     /locus_tag="PK614_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRB82283.1"
                     /translation="MNLQLSPLIELQKLDLRIADLKEQRRKIPERLEENEAPLREAKR
                     LFREASASVESLVKDRRGHEKDLEAHEDRIGKMKDRAAQLKTNQEYQAHLFEVELANK
                     KRGEIEEKILLAMEQIEQTQRTIVDVQARLKESEALFIKEKAKLDEKDRLLATELAEL
                     EVKQKHLSGQVEKSLLARYNKLKTTRKEQALALIKDGICVGCRLQLPPQLVSQVKRGE
                     DVHTCPYCYRMLYWEGDPATEAKGTVDHDQAKNLEVGESF"
     gene            complement(1765..2424)
                     /gene="rnhC"
                     /locus_tag="PK614_08485"
     CDS             complement(1765..2424)
                     /gene="rnhC"
                     /locus_tag="PK614_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRB82284.1"
                     /translation="MPASTSLSSLNRIGIDESGKGDYFGPLVIAAVFVTPALEQDLAL
                     MQVRDSKKISDGRILELAPDIRLLCPHSLVAIGPQRYNELYAKIKNLNRLLAWGHARA
                     LENLLQQVECDLAIADQFGDERLILNALQEKGKQIRLVQRTKAESDLAVAAASILARA
                     EFLQRLDRLSQELNTTLPKGASPAVELAGRMVVKKYGRDRLGTVAKLHFKTTKQVLGE
                     T"
     gene            2550..>2751
                     /locus_tag="PK614_08490"
     CDS             2550..>2751
                     /locus_tag="PK614_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRB82285.1"
                     /translation="MMRSNMLCLAGVVVAMLVTTGCQTNPYTGRWQLMMMPMSQETQM
                     GAQAYAEVKNDPKMKQSTDPREI"
BASE COUNT          646 a          753 c          830 g          522 t
ORIGIN      
        1 gagcttgtcg aagccaacct gcggttggtc gtgagtatcg ccaagaagta cacgaaccgg
       61 ggtctgcagt ttctggatct gatccaagag gggaacatcg acctcatgaa ggcggtcgac
      121 aagttcgagt ataagcgcgg gtacaaattc agcacctacg ccacctggtg gatccggcag
      181 gcgatcactc gtgccattgc cgaccaagcc cggacgatcc gcattccggt ccatatgatc
      241 gaaacgatca acaagctgat ccggacctca cgccatctgg tgcagaagct cggtcgcgca
      301 ccgaccccgg aagagatcgc cgagcgcatg gacttgccgc tcgacaaggt ccgaaaaatc
      361 ctgaaaattg cgcgcgaacc gatttccctc gaaaccccga tcggcgagga agaagacagt
      421 cacttgggcg actttatcga ggacaagaag gcggtctcgc cgttggaagc ggccattcgg
      481 tatgacttgc aacgccagat caacggcgcg ctggaaaccc tcacgcctcg agaggaaaag
      541 gtcttgcgca agcggttcgg gatcggcgag gccaccgatc atactcttga ggaagtgggg
      601 caggattttg aagtgacacg tgagcgcatc cggcagatcg aagcgaaagc cttgcgcaag
      661 ctccgacatc cgagagcctc taggcgccca gccacgggtg gtttgactct gcttaagcgg
      721 cagcctagaa tcgagcccgc tcgaaccagg tgtgggtggc gtgtgcatct cccagggccg
      781 gttggcatag gccatcgcat caggcggact atgttcagtg tactggctcg cagggctggg
      841 cccatagctc aggtggttag agcggctgac tcataatcag ctggtcctag gttcaagtcc
      901 tagtgggccc accaacctga gtgcctcacg atgaattccg tgcgaacggt gtccggtcga
      961 catcggtatc gcataaccca caaggagccc gttgaaccta cagctttccc ccctcattga
     1021 attgcaaaaa ctcgaccttc ggatcgccga tctcaaggag cagcggcgaa aaatccccga
     1081 gcggctggaa gaaaatgagg cgccgcttcg cgaggcgaag cggttgtttc gggaggcgtc
     1141 cgcttccgtc gaatcgctcg tgaaggatcg ccggggtcac gagaaggatc ttgaggcgca
     1201 cgaagaccgt atcggtaaga tgaaggaccg cgccgcccag ttaaaaacga atcaggaata
     1261 tcaggcgcat ctcttcgaag tggaacttgc caacaagaag aggggcgaga ttgaagaaaa
     1321 aattctgctg gcgatggagc agatcgagca gacgcagcga accatcgtcg atgtgcaggc
     1381 aaggctgaag gaatccgaag ccctcttcat caaagagaag gcaaagctgg acgagaagga
     1441 ccggctgctt gcaaccgagc tggccgaact agaggtcaag caaaagcacc tgtcgggaca
     1501 agtcgaaaaa tcactcctgg cccgatacaa caaactgaaa actacaagga aagagcaggc
     1561 gctggcactc atcaaggacg gcatttgcgt agggtgccgt cttcaattgc cgccgcagct
     1621 ggtctcgcag gtcaaacgcg gggaagatgt ccatacctgc ccctattgct atcggatgct
     1681 gtattgggaa ggggacccgg ctaccgaggc gaaggggact gtggatcacg atcaagcgaa
     1741 gaacttggaa gtcggtgaat ccttctaggt ttctcccagg acctgcttcg ttgtcttgaa
     1801 gtgcaacttg gcgacggtcc ctaaccggtc acgtccgtat tttttgacca ccattcgccc
     1861 ggccagttcc acggcgggtg atgcgccctt gggtagcgtc gtgttcagct cttgagagag
     1921 tcgatcgagg cgctgaagaa actccgcccg agccaggatt gaggccgctg ccacagccag
     1981 gtcggattcc gctttcgtac gttgaaccag ccggatctgt tttccctttt cttgcagtgc
     2041 attcaagatc aatcgctcgt ccccgaactg gtctgcgatc gcgaggtcgc actcgacttg
     2101 ctggagcaga ttttccaggg cacgggcatg tccccaagcc aggaggcgat tcaaattctt
     2161 gatcttggca tacagctcgt tgtatcgctg cggcccgatg gccacgagac tatgggggca
     2221 aagcaggcgg atgtcgggcg ccagttcgag gatccgccca tcggagattt tcttgctgtc
     2281 ccggacctgc atcagggcta agtcctgctc caatgccggt gtgacgaaca cagcggcgat
     2341 cactaacgga ccgaaatagt cccccttgcc ggactcgtcg attccgatgc gattgagcga
     2401 tgagaggctg gtggaggcag gcactcggtg aaactcccat gtagcaactg gccgggcggc
     2461 gcggtaatct agcagagctc accggactgg tcaatttgcg gaggcgtgtg atggaggctc
     2521 aacagaagat ggtcagaaga cggacgtcca tgatgcggtc gaacatgctg tgcctcgccg
     2581 gtgttgtcgt ggccatgctg gtgacgacgg gctgccaaac caacccctac accggacgct
     2641 ggcaactcat gatgatgccg atgtctcaag aaacgcagat gggcgcccag gcttatgccg
     2701 aagtgaagaa tgatccgaag atgaaacagt cgaccgaccc gcgggagatt g
//
LOCUS       DAORQN010000194         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1149_length_2711_cov_7.995482, whole genome shotgun sequence.
ACCESSION   DAORQN010000194 DAORQN010000000
VERSION     DAORQN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1149_length_2711_cov_7.995482"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..606)
                     /locus_tag="PK614_08495"
     CDS             complement(157..606)
                     /locus_tag="PK614_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82286.1"
                     /translation="MTVHGLPVQRACRAVGLGRATYYQPLMDWARRDARVIAALTALV
                     AVKSRWGFWKCWGRLRLDGHPWNHKRLWRVYGQLRLNLPRRTKKRLPARLRQPLGVIP
                     HPNAVWAVDFMSDTLCGGRRFRTLNLLDEGVREGLAIESDTSLPLNA"
     gene            complement(642..887)
                     /locus_tag="PK614_08500"
                     /pseudo
     CDS             complement(642..887)
                     /locus_tag="PK614_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1064..1666
                     /locus_tag="PK614_08505"
                     /pseudo
     CDS             1064..1666
                     /locus_tag="PK614_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889101.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1878..2243)
                     /locus_tag="PK614_08510"
     CDS             complement(1878..2243)
                     /locus_tag="PK614_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82287.1"
                     /translation="MIQISRSLSLVIISMLLAACSHFQNREAAYLDSAKGWATQAQVR
                     EKLGAPKTVRQDEGGATVWVYELREQQSGNRLTAPGMWCEQYLLTFDDKAVLQKWRQV
                     SHFHGGELMPQECIPGTEK"
     gene            complement(2502..>2711)
                     /gene="rpmB"
                     /locus_tag="PK614_08515"
     CDS             complement(2502..>2711)
                     /gene="rpmB"
                     /locus_tag="PK614_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRB82288.1"
                     /translation="EQRSRAVAFACDLCGKKHQTGNNVSHANNKTKRVFNPNLQRVKA
                     LVNGSALRIRVCTRCLRSGLVKKAV"
BASE COUNT          525 a          800 c          789 g          597 t
ORIGIN      
        1 aggcattctg atccggcttg cccggttgga tgtgccacag ctgaatcgcc cgctacgcgc
       61 accaggtgac gaaccggtcg gggagaaatc ctgggccgtt gtcgagccga atcgcctggg
      121 gttgcccccg ccagctcacg actcgctcca cgcgaatcac gcgttcagcg ggagcgacgt
      181 atcgctctca atcgccaagc cttcccgcac gccttcatcc agcaggttca gcgtccggaa
      241 tcgtcggccg ccgcacaggg tgtcactcat gaagtccacg gcccacacgg cgttcgggtg
      301 cggaatcacg cccaacggct gtcgcagacg agcggggagc cgcttcttgg tccgacgggg
      361 gagattcaag cggagctggc cgtacacccg ccagaggcgc ttgtgattcc agggatgtcc
      421 atccaatcgg agtcgccccc aacacttcca aaagccccag cgactcttca ccgcgacgag
      481 cgccgtcaac gccgcgatga cccgcgcatc ccgccgagcc cagtccatga gcggttggta
      541 gtacgtcgcc cgtcccaaac ccactgcgcg acaggcccgc tgaaccggga ggccgtgtac
      601 ggtcacgaga tgcgtgacaa cctcctgacg ttcagcgggc ttcagagctt ttttgcgatg
      661 acatctttca gcgccgcatt ctcgagcgag agatcggcat acatccgctt gaggcgggtg
      721 ttttcgtgtt ccacttcctt cagtcgcttc acatccgagg cttccagcct gccgtacttg
      781 gctttccact tgtagtaggt cgcggaactg atgccgtgct gccgccacaa ctcatggacc
      841 gggcgcccgg cgtcggcttc tttcaggatc gacacgattt gggtctcggt gaacgtcgct
      901 ggtgcatggg aacctcctgg ctagggtgac gattgtgccg gaatattctc ctaatggctg
      961 tctcgatcta tggggagctt acggtctgtc gacgatgcgg ggacaaccac gttcctccgt
     1021 tggtgcgtcc ggcgcggcag aagacgcgac accgctgtct gctcggctca ttcccgggga
     1081 ccgcgtgcag ctcgacacct gcaagattgc tccgggatgc tatcagtaca cggcggttga
     1141 cgattgtacg cgttatcggg tgctagggat ctttcatcga cgcacggcgg caagtacggt
     1201 ggcattctta gagcgtctcc tggaggagat gccgtgtccg gtgcagcgga ttcaggccga
     1261 tcgaggctga gaattctttg cggagtcggt ccaacagtgg ctgaaggacc acggcatcaa
     1321 attccgtccc atcaagctcg cctcaccgca tttgaatggc aaagtggagc gctctcagga
     1381 gaccgatcgt gaggaattct gggcgactgc tgacctgaac agccctgacc tggaattgcg
     1441 ggtggccgag gagcaacact atgacaactg ggatcggcct catggatcgt taaatggcca
     1501 gacacccatc gcgatggtgc acacgaacag cgagaacacg ctgtttgggt atgacgttga
     1561 agcgaagtac gaccctggca aggagcgatt ccaggtagct cactaccaaa cagacttggc
     1621 cctaaggcgg catgttcaag caccgcacca ggctggaaca cagtgatagg atgtctatga
     1681 atcacacact ttatttagct gcgtgggtac ttttcgatgt aaacatgtcg tcccatctgc
     1741 tcccaacctt tttggcctac tgaccaaaac cttccagcgc cttttaagcc ttttcccgtt
     1801 tggactccct ccttttcact gttcccttct gcatttacag acgctcgtac atcggtctac
     1861 cagttttggt gtggccacta tttctctgtt cccggaatgc attcctgcgg catcagttcg
     1921 ccgccatgga aatgggacac ctgtctccat ttctggagca cggccttgtc gtcgaaagtg
     1981 aggagatact gttcgcacca cattccgggt gcggtcagac ggttcccgga ctgttgctcg
     2041 cgcaattcat acacccacac ggtggctccg ccttcatcct gtcgaacagt cttgggagcg
     2101 cctagttttt cccgcacctg ggcttgagtg gcccacccct tggcggaatc cagataggcc
     2161 gcttcccggt tctgaaaatg gctgcatgcc gcgagcagca tggagatgat gacgaggctg
     2221 agcgaccgac tgatctgtat catgttgcct cccgattcga ctgggatccg tttttgccac
     2281 ccgcttgtct ggcaggggat tgtacacgga tagcgggggc gcgtctcctt tggagaagat
     2341 ttcctgcatc gtccctggtc tccgtgagtc cgaggcggca gactattcgt gggggtgtgt
     2401 gaggccgtac cgaatctgaa gaagagcgag cggggagaag gcgcaactta ggcgtgagat
     2461 cagagcacga gctgacgaga tgggggagaa atgcggagca atcagacggc ttttttaacc
     2521 aggccggacc gcaggcaacg cgtgcaaacc cgaatgcgca aggccgaccc gttcacgagc
     2581 gccttcactc gctgtaggtt gggattgaag acgcgcttcg tcttgttgtt tgcgtggctg
     2641 acgttattac cggtttgatg ctttttccca cagagatcac aggcaaatgc cacggcccta
     2701 ctcctttgtt c
//
LOCUS       DAORQN010000195         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1156_length_2692_cov_24.866136, whole genome shotgun sequence.
ACCESSION   DAORQN010000195 DAORQN010000000
VERSION     DAORQN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_2692_cov_24.866136"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PK614_08520"
     CDS             complement(<1..1011)
                     /locus_tag="PK614_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB82289.1"
                     /translation="MGNRTSLTDRRGAQAFGYDQLDRLTSASHPLLLDPQAFAYDAVG
                     NRTTNGSIVNVGNQLTADANFAYQYDDNGNLTRKTLLVTGNYTQYTYDAENRLTQVQE
                     FGAGNPTAITTSTYRYDGLGRRIEKVANGQTKRYIYDGEDILLEYDGSNALLARYTHG
                     PGIDEPIAVTKAGSTFYYHQDGLGTVTELTDTNGTVAKAYAYDAYGSILDSPGTVDQP
                     YTYTGREFDSETGLYYYRARTYDSSSGRFLQKDPILSPTNQLMGEEGMFFGRTTWLLP
                     AYLDETRMLNPYSAVFNSPLNFIDPEGLIPLVPCSIEEQLTKFCKKVQKVGKYARLVV
                     VLC"
     gene            complement(1165..1509)
                     /locus_tag="PK614_08525"
     CDS             complement(1165..1509)
                     /locus_tag="PK614_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82290.1"
                     /translation="MKKHQGRPRRAWLFSAGLLLCISCNNQDLEYERTLAACMKVQIG
                     DTEQRVRELMGEPMKAEQRIANSGKGRVLGFPAPAIIASAPQIYLDETGRVDEIICYD
                     SHRLTKEIKNQS"
     gene            complement(1607..1831)
                     /locus_tag="PK614_08530"
                     /pseudo
     CDS             complement(1607..1831)
                     /locus_tag="PK614_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1794..2267)
                     /locus_tag="PK614_08535"
     CDS             complement(1794..2267)
                     /locus_tag="PK614_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82291.1"
                     /translation="MTTFAYDSKNRLTQVTDPNTGVTAYAYDGNGNLLTVTDAKNQVT
                     TFAYDGRNRLLSTTDPLGKIESYTYDGNDNLIKRRTPKGDDILFAYDAVNQLLSKTLP
                     GSQVTSYLYDQVGNLTNVTDPDSVLAMTYDLANRRLAWVLGVSPGRVHTSRPRHR"
     gene            complement(2316..2657)
                     /locus_tag="PK614_08540"
     CDS             complement(2316..2657)
                     /locus_tag="PK614_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82292.1"
                     /translation="MVKPSLAQYVLAIVLGALGMVSCHSKDFAIVDGDVNVLENPLPL
                     SFSESKTYANPPATLPANRIVAVLRKGQTIELLGRKYGKDFLAYEVQLPDGRRGYLIH
                     GDSFLLSGSPE"
BASE COUNT          565 a          705 c          733 g          689 t
ORIGIN      
        1 gcacaacaca acgaccaatc gagcgtactt tccgactttc tgaaccttct tacagaactt
       61 ggtgagctgc tcttcgattg agcacggaac caatggaata agcccttcgg gatcaatgaa
      121 gttgagcgga ctattgaaga ccgcactgta gggattcagc atcctggttt cgtcgaggta
      181 tgccggcagc aaccaagtgg ttcgcccaaa gaacattccc tcttcgccca tcaactgatt
      241 agttggcgac aggatcggat ctttctgtag gaatctcccc gagctcgaat cgtaggttct
      301 cgctctgtag taatagagcc ccgtctccga gtcgaactcc cgtcccgtgt acgtatacgg
      361 ctgatccacc gtcccgggcg agtcgagaat gctgccatac gcatcatagg cgtaggcctt
      421 ggcgactgtg ccgttggtgt cggtcagctc tgtgaccgta cccaggccgt cttggtggta
      481 gtagaacgtc gaaccagctt tggtcacggc aatcggctca tcgatgccgg ggccgtgggt
      541 atatcgagct agcagcgcgt tgcttccgtc gtactccagc agaatgtctt caccatcata
      601 aatgtagcgc ttcgtctgcc cattggcgac cttctcaatg cgccggccca agccgtcgta
      661 gcgatacgtg ctcgtcgtga tcgccgtggg attgccggcc ccgaactcct gcacctgggt
      721 caggcgattt tctgcatcat acgtgtattg ggtgtagttc ccggtcacca gcagcgtctt
      781 gcgcgtaagg ttcccattgt cgtcgtactg gtaggcgaag ttcgcatcgg ccgtcagttg
      841 attgccgaca ttcactatgc tgccattggt cgttctgttc ccgacggcat cgtaggcaaa
      901 cgcttgtgga tccaaaagca gcggatggct ggcactggtc agccgatcca gttggtcata
      961 cccaaacgcc tgcgccccac gcctgtccgt caagctcgtc cgattgccca ccgcgttgta
     1021 cccgtattcg gctttgttga ttgatcaaat tgtcgttgcc gtcgtaggtg taggtttcga
     1081 tcttacccaa gggatcggtg gtgctgagca atcggttccg gccgtcgtag gcaaaggtgg
     1141 tgacctggta accaacatta acatttaact ctggtttttg atttcttttg tcagacggtg
     1201 cgagtcgtag cagattattt catcaacacg gccggtttca tccaggtaaa tctgaggagc
     1261 gctagcaatg attgctggag cggggaaacc taacactcgg cccttccctg aattggcaat
     1321 ccgctgctca gctttcattg gctcacccat taattctcgc actctctgtt cagtatctcc
     1381 tatttgtacc ttcatgcacg ccgctagtgt tctttcatac tctaaatctt ggttattaca
     1441 gctgatgcac aggagtaatc ctgcggagaa cagccatgca cgcctggggc gtccttgatg
     1501 tttcttcata cggctgccct cgcttacgtt tttatttgca ggtacatgct tattactttg
     1561 actccggtga cagcaattcg gtgagggaaa tggggctttt ctgtgccgag attcccagca
     1621 aggtttgaaa ggcagccatc ggagtcttac gccggttgaa gcgaaagaca aactcgtcca
     1681 aatagctcgg aagatgcttg ggctcaaccc cgtgatgcgt ggcgttcagc cacgccttga
     1741 gattgctgaa cacgcgatgg acgtgaggaa agcgtcgagc ggcttggcgg ggctcaccga
     1801 tgacgcggac gtgatgtgtg tactcttcca gggctgactc cgagtaccca cgccaaccgt
     1861 cggttcgcca ggtcgtaggt catggccagt acgctatcgg gatcggtcac attggtcaag
     1921 ttgccgacct ggtcatagag gtaactcgtc acctggctcc ccggcaaggt cttgctgagc
     1981 aattgattca cggcgtcgta ggcgaacagg atgtcgtcgc ccttcggagt ccgccgctta
     2041 ataaggttgt cgttgccgtc gtaggtgtag ctttcgatct tacccaaggg atcggtggtg
     2101 ctgagcaatc ggttccggcc gtcgtaggca aaggtggtca cttgattctt ggcatcggtg
     2161 acggtcagga gattgccgtt gccgtcgtag gcataggccg tgacacccgt gttgggatcg
     2221 gtcacttgag tgagccggtt cttggaatca taggcaaacg tcgtgatgcg ggactatgac
     2281 atgacccgat tccaggttca gaccgccaga cgggattatt ctggcgaacc ggagaggaga
     2341 aagctatctc catgtattaa gtatcccctc ctgccatcag gcaattgcac ttcataggcc
     2401 aggaaatctt tgccatattt ccgaccaagg agttcaattg tttgcccctt ccttaacaca
     2461 gccactatcc gattagccgg aagcgtagcg gggggattag catatgtttt agattcggaa
     2521 aaagagaggg gaagtggatt ttccagtaca ttgacgtccc catcaactat ggcgaaatct
     2581 ttgctatgac agctgaccat tcccaatgca cccagaacga ttgcaagaac gtattgagct
     2641 aacgagggtt ttaccatgat taatttctac ttggggtgag aaggagttga ag
//
LOCUS       DAORQN010000196         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1164_length_2675_cov_8.008015, whole genome shotgun sequence.
ACCESSION   DAORQN010000196 DAORQN010000000
VERSION     DAORQN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1164_length_2675_cov_8.008015"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1335)
                     /locus_tag="PK614_08545"
     CDS             complement(154..1335)
                     /locus_tag="PK614_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRB82293.1"
                     /translation="MTFEPHKFFIGLVDFFSILMPGALLTYLGKDWASTTFPNLFCFD
                     SEGVEGLAVFFFSSYILGHFLFLIGSLLDDLVYEPVRNATGPKQVARLLLGRKLSAKP
                     LRWFARVCFKTQPDRAVDRVVALKTDCLSKIGNAEVVNAFQWCKARLTMEQPEALAIV
                     NRLEADSKFFRSFVPMLAGLFLFTLGHAIYRLTIPTQAAPSDGWLIVGSALLLLLAFW
                     RYMEQRFKATQQAYWFVLTMEASQLTKNPNASSSPVPSKAATRTDELTHAGGVVVRQR
                     NGKREYLLVQAKNNPSERVLPKGHIEPEEDPRHCAIREVHEETGVWARIARPLKVNEY
                     TVLNESVKAQFYLMDAVDEGKAEDPERKTEWLSLEEALERTKPIEQTYNLFRQLQREA
                     Q"
     gene            complement(1861..2199)
                     /locus_tag="PK614_08550"
     CDS             complement(1861..2199)
                     /locus_tag="PK614_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82294.1"
                     /translation="MTTKQDHAMDSHELAAKYQPLSDGRRAKLVGQPYPVPPRDRTTP
                     ELLKGYQFALITTHGPEFPEFDIPLTYLRDRGASVDVVTQGRGKWWQEPLFEGLRVER
                     GDVECGRQVS"
BASE COUNT          622 a          718 c          654 g          681 t
ORIGIN      
        1 atcgataccc cttcagcaga tctggagtag ctctgtccct tactggaacc ggataggcgg
       61 tgcctatcag cttttctttc aatccatcag gaagtggctg atattttttt cctaatgcat
      121 gagaccctgt cgcgcggtcg tcttttattg gagtcattgt gcctctcgct gtaactgacg
      181 aaacaaattg taggtctgct cgattggttt cgttctctcc agggcttcct ccaaggacaa
      241 ccattcagtc ttgcgttccg gatcctccgc cttgccctca tcgactgcgt ccatcaggta
      301 gaactgagct ttgaccgatt cgtttagcac ggtatattcg ttcactttga ggggtctggc
      361 gatgcgcgcc cagacgccag tttcttcatg cacttcacga atagcgcaat gtcggggatc
      421 ttcttccggt tctatgtgac ctttgggaag cacccgctca ctaggattgt tctttgcctg
      481 aaccaagagg tattcgcgct tgccattgcg ttgcctgacc accaccccac ctgcatgtgt
      541 gagctcatcg gttctggtcg cagccttact ggggacaggt gatgacgagg cattgggatt
      601 cttggttagc tgactggctt ccatggtgag gacaaaccaa taggcttgct gggtggcttt
      661 gaagcgctgt tccatgtaac gccagaaggc taagaggaga agaagtgccg atccgacgat
      721 cagccaccca tcggaaggcg ccgcctgcgt tggaatcgta aggcgataga tcgcatgccc
      781 cagagtgaat aggaatagac cggcgagcat aggcacaaaa ctgcgaaaga attttgagtc
      841 cgcttcgagt cgattcacga tcgccaacgc ttctggctgt tccatggtga gtctggcctt
      901 gcaccattgg aaggcattaa cgacttcagc atttccaatc ttggacagac agtctgtttt
      961 gagtgccaca actcgatcga cggctcgatc cggttgtgtc ttgaaacaca cccgagcgaa
     1021 ccagcggagg ggtttagctg aaagtttcct gcccagcagc agccgtgcaa cttgtttggg
     1081 gccagtcgca tttcggaccg gctcatagac cagatcgtcg aggagggagc cgatcaaaaa
     1141 gagaaaatgg cccagtatgt agctcgaaaa gaaaaagact gccaggcctt cgactccctc
     1201 cgaatcgaag cagaacaagt tggggaaagt cgttgacgcc caatcctttc ccagataggt
     1261 caagagcgct ccgggcatga ggatggagaa gaaatcgacg aggccgatga agaatttgtg
     1321 cggttcgaag gtcacaatag gtctttcaca acgcccttgc ttacacagct cacttgtccg
     1381 gtcaacagcg caacgagtgg atcgcctggc cctcaaacaa caaatcgaat ttggtcaccg
     1441 tctcgggcat gacacgtgtg agccattcct tagaaggcat atttcgttgg tgtattcgaa
     1501 acgcagccct gtttgtatag ccttcacaga catagaacgc atgacgatta tggggatccc
     1561 ggaacaactc gaattgccga gtgccctttt cgtcacgctt ggcgactcgc gcatcctttt
     1621 ttatgaaggc aataaaagcc ctgcgtttgg caggtcggac ttcgactcgg aagagaataa
     1681 ccaacataac tgctctctca gatggggtag ctcattacca aatcagctac atggcggcca
     1741 aatctcaata ggtacggacc attacccgcc tggtctaaca attgaaagga aatcggccac
     1801 gcagatgatg aatctctgtg gctccaagct catacgtatc gttcgcctct caagcccgta
     1861 tcagctcact tgcctcccgc attcaacgtc gccacgttcg acacgtaaac cttcaaacaa
     1921 tggttcctgc caccatttcc ctcttccttg cgtcacgaca tcgaccgacg ctcctcgatc
     1981 acgcaggtat gtcagtggga tatcgaattc tggaaattcc ggcccatggg tcgtgatgag
     2041 ggcaaattga tagcccttca acaattctgg agtggttctg tcacgtggag ggaccggata
     2101 gggttgacct accagttttg ctctgcggcc atcagaaagc ggctgatact ttgccgccaa
     2161 ttcatgagaa tccatcgcgt ggtcttgttt tgtggtcatt gcccctcctc ctgttccagc
     2221 ttcagaaaca agtcaccggt ctgtttgatg agcaaggaca accattcatg tttgcgttca
     2281 cgatcttcag gctcgtcttc ttccactact tctatgaaat agaagtgaac atttactggt
     2341 cttgtcggaa ggattgctaa taaaggtcct gagatttttt ttagccctta taccctcctt
     2401 aggttacgcg cggctgaaca attgagcttc cagctagtcc tacgtttact ccacgaccag
     2461 gcaaatagct aaaactttgg tccatacgag gttgcaagcg ctatagaagc atacgacgcc
     2521 ccctccactc gcattctccc tgcaatcttg gactcggatt cagctctcgc gtgccctgct
     2581 gccatactta gatcaaatac agtttctaga tccattgggc caatgcctat ctcacccgca
     2641 attgctctag agacccgttc tgcagcactt gcatc
//
LOCUS       DAORQN010000197         2671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1165_length_2671_cov_27.259939, whole genome shotgun sequence.
ACCESSION   DAORQN010000197 DAORQN010000000
VERSION     DAORQN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_2671_cov_27.259939"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PK614_08555"
     CDS             complement(<1..1016)
                     /locus_tag="PK614_08555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRB82295.1"
                     /translation="MNFNSVEFFVFFSCLLALYALVFHRVLWRDLLLLVSSYFFYMCW
                     NWKYAGLLALSTCVDYSVGRLLARATDPRSRRWILIISLSSNLGLLALFKYYNFFMDV
                     AKPAASAFGWDLAFLQHQLLLPVGISFYTFQTMSYTIDVYRGERVLERNFLKFALFVA
                     FFPQLIAGPIVRARQFLPQLHHPPAVTFERFHSGLFLVFKGLFKKIVFADLLAALAVD
                     KVFSDPLSFSSWDLLIALYAYSFQIYYDFSGYSDIAIGLARMLGYDLPQNFNRPYLSQ
                     NAREFWTRWHISLSTWLRDYLYIPLGGNRAAPWRVKLNLMITMLLGGLWHGAAMHFVL
                     WGGF"
     gene            complement(1013..2086)
                     /locus_tag="PK614_08560"
     CDS             complement(1013..2086)
                     /locus_tag="PK614_08560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB82296.1"
                     /translation="MTFQLLKILWWGLLFLLMAEVGLEFRAYHRGFDTIAFGSYQRQG
                     AEPNAPAVSPTIQPASLGDQLGEKNAGEIRYWIASSSHAEDSYLSRELIFPTRLEGLL
                     RESGQLAVVVNASHAGMTIQANQTDLEGRGNRIHPNIVILYQMSTEITELSKMLLSPR
                     RSESTKAKDSGSALSPPAQPNMIVRAFEHTSIYALLKGNLSNRLAGQRVLADSLGGEG
                     DAAFEQQVLAFVQTARRIGAEPVLCTFATSHTRSQFPNVPDAVSSFLFRYNMYLSVIG
                     WLDAIDRFNDVLRRIAAKEHFRLVELDTVLSGREENFRDFVHFTPEGHARVAQAIFSQ
                     LRPGSSSAVRLAAPNAGRGRQTP"
     gene            complement(2392..>2671)
                     /locus_tag="PK614_08565"
     CDS             complement(2392..>2671)
                     /locus_tag="PK614_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82297.1"
                     /translation="VKKSTTNTSRDGIVRWWVNGVLVGNYTTLNYAGAGLNEWVWSEA
                     WDGTFNPNHVEWNHFIDHLHVSIPNGGGSTDQPPGPPASPSIRSVTAP"
BASE COUNT          662 a          695 c          787 g          527 t
ORIGIN      
        1 tggaatccgc cccagagcac gaagtgcatg gcggctccat gccaaagccc tccaagcagc
       61 atggtgatca tcaagttgag cttcactcgc cacggagctg ctcggtttcc gccgagtggg
      121 atgtagagat aatcccgcaa ccaagtcgac aatgagatgt gccagcgtgt ccagaactcg
      181 cgagcgtttt gcgagagata gggacgattg aagttctgag gaaggtcata ccccagcatt
      241 cttgccaggc cgatggcaat atcgctgtag cctgaaaagt cgtagtagat ctggaacgag
      301 taggcgtata acgcgatcag cagatcccag gaagaaaagc tgagcggatc cgaaaacacc
      361 ttatcgacag cgagtgctgc cagcaggtcc gcgaacacga tctttttgaa caaacctttg
      421 aagaccagga acagcccgct gtgaaatcgc tcgaaggtga cggccggcgg atggtgcagc
      481 tgcggcagga attggcgggc gcgtacgatg ggccccgcga tcagttgcgg gaagaaggcc
      541 acaaacaggg caaactttaa gaagttgcgt tcaaggactc gttcaccacg gtacacgtcg
      601 atcgtatagc tcatcgtctg aaacgtgtag aacgagatgc cgaccggcag gagcaactgg
      661 tgctgcagga aggcgaggtc ccagccaaat gcggaggcgg ctggtttcgc cacgtccatg
      721 aagaaattgt aatacttgaa tagggccaac aggccgaggt tggagctcaa actgatgatg
      781 aggatccagc gtctcgatcg agggtcggtg gcccgagcaa ggagacgacc gacgctgtag
      841 tcgacacagg tggacagagc cagcagtccg gcatacttcc agttccagca catgtaaaag
      901 aagtaactcg agaccagcaa taggaggtcg cgccagagga cgcgatgaaa cactagggcg
      961 tagagcgcaa gtaggcacga aaaaaacacg aaaaattcga cagagttgaa attcatggtg
     1021 tctgcctgcc ccggccggcg ttcggggcgg ctagccgtac cgcggaagaa gatcctggcc
     1081 ggagttgtga gaagatcgct tgcgcgacgc gtgcatgccc ctcgggcgta aaatggacga
     1141 agtcgcggaa attttcctcc cgacccgaca acaccgtgtc aagttccaca agccggaagt
     1201 gttccttcgc cgcaatacgg cgaaggacat cgttgaagcg atcaatcgca tccagccatc
     1261 ctattacgga cagatacatg ttatatcgaa aaagaaaact tgaaacagca tctggcacat
     1321 tgggaaattg cgatcgcgtg tgacttgtgg caaatgtgca gaggactggt tcggcgccga
     1381 tgcgtctggc cgtctggacg aaggcgagga cctgctgttc aaatgcggca tcgccttcgc
     1441 cccccaagct atcggccaag acccgttgac cggccagtcg gttcgagagg ttgcccttta
     1501 ggagcgcata gatggaggta tgctcgaatg ctcgtacgat catgtttggt tgcgcgggcg
     1561 gcgatagcgc ggaaccggag tccttggcct tggtcgattc cgaacgtctt ggcgacaaga
     1621 gcatcttgga gagctcggta atttcagtcg acatctgata gagaatgacg atgttcggat
     1681 ggatgcggtt cccgcgtccc tcgagatcgg tctggttcgc ttgaatagtc attccggcat
     1741 ggctggcatt gaccacgacg gccagttggc cggactcacg aagcaagcct tccaagcgag
     1801 ttgggaaaat cagctcgcgg ctcaagtagc tatcttccgc gtgtgaggag gaggcgatcc
     1861 agtagcgaat ctctcctgca ttcttttccc ctagctggtc tccaagactg gctggttgta
     1921 tcgtcgggct aactgcgggt gcgttcggtt cagctccctg tcgctgatag ctgccgaacg
     1981 cgatggtatc gaacccccta tggtaggcgc gaaattccag accaacctca gccatgagaa
     2041 ggaagagaag gccccaccac aaaattttaa gcaattgaaa cgtcatagcc ccagccctcg
     2101 attaccgagc catatcgacg aggcaaccct atggaggctc agaagcctat atagaaagcc
     2161 agtaagctgg taatgggggc taaacgtgcg cggcatgagg tttcagacca tagcatgtca
     2221 caaacggtga ggggcttggg ataatgagag atcgctgagg ggaacaccca acggttgctg
     2281 caaatagaaa gaaacatgat acaaggcgca ccggtccgtt gccccccgtt caattttggc
     2341 aggctgtgta atcagcatag cctcaaggct cgatgaaggg tcacgcacca cctagggtgc
     2401 tgtgacgcta cggatgctgg ggcttgcagg cggccccggc ggctggtcgg ttgagccgcc
     2461 tccattggga atcgacacat gtaggtggtc gataaaatga ttccattcga catggttcgg
     2521 attaaaagtc ccgtcccacg cttccgacca gacccactca ttaagccctg ccccagcata
     2581 gttcaaggtc gtgtaattgc ccacaagcac accattgacc caccaccgga caatcccgtc
     2641 gcgtgaggtg ttggtggtgc ttttcttgac g
//
LOCUS       DAORQN010000198         2643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1173_length_2643_cov_25.598918, whole genome shotgun sequence.
ACCESSION   DAORQN010000198 DAORQN010000000
VERSION     DAORQN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1173_length_2643_cov_25.598918"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1184
                     /locus_tag="PK614_08570"
     CDS             183..1184
                     /locus_tag="PK614_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82298.1"
                     /translation="MNRALLSMVMLLMVTSTVYADGGHDHHAIPTMPSLGDTKITIDD
                     HTVTLTFGPVDLPTGHDGDLASSMPKKVFQLPDDTYMIGYKSEVFTKDGKPLPKNYLH
                     HILMLNNDKPSVSCDGEPLFFAGAGLEMTEARFPDGYGVKLGKGQNLMSVVAFYHKAP
                     PTKDVMARFTMYVAPKGTKVQEMDVYQVGVNIVCYSKFGQRGADQTDEGIEIKPGVSV
                     LSAPLKFNMDGCVKFAYPHGHDELLMVALENKSSRETLLRTVPEVGADGTFRDFRPHQ
                     VYRDDKGFTVTKRDDYEMVMVHHHPLQNPNIQHGMGNYLLYMTPGVCPQSANTASAR"
     gene            1575..>2643
                     /locus_tag="PK614_08575"
     CDS             1575..>2643
                     /locus_tag="PK614_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB82299.1"
                     /translation="MKSSMSPAEPSLNILLINEQPDEIKLVTSSLRGFFPGCRIEAGY
                     SSEEALTFSHQGEWHIILIDHDLRPERGLDILSRLRRNAPYAAIILQTNESDSQTAVQ
                     ALQNGADFLLFKRSPAFITELLFSVQEAVEKRDLQMKLDHTFQRHQRFIETLSDLVYE
                     LDRDGRFVYVGTAVTAMLGYTPEELAGRHYSLLLPPLQEATGRFRLNERRAGSRSVRR
                     LELTLHRKALPDAPPASITVDVTAKGLFDSANRYLGTVGVLRDLSQQKAQQDRLTELE
                     SRLQETTRQLTLSREAARVSRQLQQPLTTLLQDSQRLLSSIQDSKIEQHVETMIEKAS
                     QASQLGHQLAQAIHAPPLEFFP"
BASE COUNT          606 a          840 c          664 g          533 t
ORIGIN      
        1 gggaatctgg tggtacgtgc gtgtcggcac cagagacaat attccgcccc ggcaacatgg
       61 cggtacttac ctgtggaagc gactgacccc gcccccgacg gcaccctaac gggtgggcct
      121 atttcccgac attgtctcac acacactcac ggacggccgg tctgttataa cgaggagggg
      181 ctatgaatcg tgcgctgcta tcgatggtca tgctgctgat ggtcacaagt acggtctacg
      241 ccgacggtgg ccacgatcac catgccattc caaccatgcc gagtttaggc gacactaaga
      301 ttaccatcga tgatcatacg gtcactctca ccttcggtcc ggtcgatttg ccgaccggtc
      361 atgacggcga tctggcatcc agtatgccca agaaggtctt ccagttgccg gacgacacct
      421 acatgatcgg ctataagtcc gaggtcttca cgaaagacgg caagccgctg cccaagaatt
      481 acctccatca catcctcatg ctgaacaacg acaagccgag cgtgtcatgc gacggcgagc
      541 cgttgttctt cgccggtgcc ggcttggaaa tgacggaagc acgttttccg gacggatacg
      601 gggtcaagct gggaaaaggg caaaacctca tgtccgttgt ggcgttctac cacaaggctc
      661 ccccgacaaa agacgtgatg gcccgtttca cgatgtatgt cgcgccgaaa ggcacgaagg
      721 tgcaggaaat ggatgtctac caggtcgggg tcaatattgt ctgctacagc aagttcggcc
      781 agcgtggcgc ggaccaaact gacgaaggca tcgaaatcaa gccaggggtt tcagttctgt
      841 cagccccttt gaaattcaac atggacggct gcgtcaaatt cgcctacccc catggccacg
      901 atgagttgct gatggttgcc ctggagaata aatcctccag agaaaccctc ctgcgaacgg
      961 tccctgaagt gggtgccgac ggcacgtttc gtgactttcg ccctcatcag gtgtaccggg
     1021 atgacaaagg gttcacggta accaaacgcg acgactatga aatggtgatg gttcaccacc
     1081 acccgcttca aaacccgaat attcagcatg gaatgggaaa ttacctgctg tatatgaccc
     1141 ctggcgtttg cccgcaatct gccaacacag cttcagctcg ctaggcacag gagagttccc
     1201 cgcccaccgc ggtccaacga ttgcggtggg catcccgttc gttcccgaag ctgccggcac
     1261 atcatccgac cggcgaaacc ctcgccccga cccccctccc caacgagaat tgcaccttat
     1321 tcttggggtg tgatcactct tgcttgctcc gcacagcaca ctcccctact gaagtaaacg
     1381 cgccccctca agcagcgggc tgggattctc aataaggtgt atgatattga accaatcatg
     1441 cactccaacc gactacctgg ggacacaatt gtccctctga cagcgagcgc atgttcatca
     1501 ctgtgcggtg gtgcattccc aggcgcccgc ccggcggcat cgaagcagaa cgatcgcacc
     1561 actgcatagg aaccatgaaa tcttccatgt cgccggctga accgtccctc aacattctcc
     1621 tcatcaacga acaaccggat gaaatcaagc tggtgacctc cagcctgaga ggatttttcc
     1681 ccggctgccg gatcgaagcg ggctactcat ccgaagaagc cctgacattc agccatcagg
     1741 gcgaatggca catcatcctc atcgatcacg atttacgccc tgaacgcggt ctcgacattc
     1801 tttcccgcct tcgtcgcaat gctccctatg cggccatcat cctccaaacg aatgaaagcg
     1861 attcccaaac cgccgtccag gcgctgcaaa acggcgctga cttcctcctg ttcaaacggt
     1921 cacccgcctt tatcaccgag ttgttgtttt ccgtccagga ggcggttgaa aagcgcgacc
     1981 tccaaatgaa actggaccac acctttcaac ggcaccagcg gttcatcgaa acgctgagcg
     2041 acttggtgta cgagcttgat cgggacggtc gctttgtcta cgtgggtact gccgtcaccg
     2101 ccatgctcgg ctatactccc gaggagcttg ccggacgaca ctattcgctt ctactccctc
     2161 ccctccaaga ggcaacagga cgctttcgcc tgaacgaacg acgggccggc agccgctcgg
     2221 tacgcagact tgagctcact cttcatcgga aagccctacc agatgcacca cccgcatcga
     2281 tcaccgtcga tgtaacagcc aagggactct tcgattcggc caatcgttat ctcggcacgg
     2341 tcggggtcct gcgcgacctt tcccaacaga aagcgcaaca ggaccgactc accgagttgg
     2401 agtcacgcct ccaggaaacc acccgtcaac tcacgctttc ccgggaggcg gcccgggtat
     2461 cccgccagct ccaacagccc ctcaccacgc tattgcagga ttcgcaacga ttgctcagct
     2521 caattcagga cagcaagatc gagcagcatg tcgaaacgat gatcgagaaa gcgtcgcagg
     2581 ccagccaact tggccaccaa ctggctcaag ccatccatgc ccctccttta gagtttttcc
     2641 ccc
//
LOCUS       DAORQN010000199         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1178_length_2632_cov_7.314707, whole genome shotgun sequence.
ACCESSION   DAORQN010000199 DAORQN010000000
VERSION     DAORQN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_2632_cov_7.314707"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PK614_08580"
     CDS             <1..494
                     /locus_tag="PK614_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82300.1"
                     /translation="SSQVLALDGWVVAPVTVEFSSISTPVLAMLLVGAGALGLLIARL
                     CGKGLRARYYKTWGCGLNLTPRMEYTATGFAQPIKQVFETIYQPTVKLEQEFLEQSKY
                     FIKHQRFESHIEPVFEKYLYRPVVTLLLATADRLRIIQAGSLHLYLTYIFVTLVLLLL
                     WAG"
     gene            559..1515
                     /locus_tag="PK614_08585"
     CDS             559..1515
                     /locus_tag="PK614_08585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRB82301.1"
                     /translation="MQSIIHIVLVVVQLVLLLTLSPFVVGLIRKVKARLQCRRGASLF
                     QPYADLAKLFRKQPVISSTTSWIFTAAPYIVFASTVAAGLLMPVFVSHTPLNFAGNII
                     ALVYLLALGTFFLILAGLDAGSAFGGMGSSREAIVATLAEPAMMLSILSIALTAGSTN
                     LSTIVHQSALMEGVVVAPPAHLMALAALFIVTLAETGRVPVDNPATHLELTMIHEAML
                     LEYSGRYLALMEWAAGIKLLVFLTLIVNVFAPWGIATSLAPSAIAVGLLVYLGKVAAL
                     ALVIGVIESMFAKLRLFRVPEMLGAAFILSLLALVFFYTLKG"
     gene            1520..2185
                     /locus_tag="PK614_08590"
     CDS             1520..2185
                     /locus_tag="PK614_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRB82302.1"
                     /translation="MPASTQLGSQLVDLCSVLLLLCCFAIVAQRRLSACVDLFALQSA
                     FLAATAALVAFLTGHKHIYFAAALTIVIKVIVLPRILRKVIERLNVSRELGMNINIPA
                     GLLICGALVIIAFFITQPIIPLGYLLTRDSLAIALAIILIGFFTMIARQKAITQMVGF
                     LVMENGVFLGATAAAYGMPLIVELGVFFDVLVAGLIVGIYTHRLQDTFDSVDTSTLTV
                     LKE"
     gene            2223..>2632
                     /locus_tag="PK614_08595"
     CDS             2223..>2632
                     /locus_tag="PK614_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82303.1"
                     /translation="MWSVIVLLTGPVLAGLLSLVIHRARVLHVVNFSTMLALAVAETA
                     LTRQVLAEGSVTTLGGFVYVDALSDFILMIITAIGLSCSLYMWSYMDDRVARGIIAPK
                     RLGHFFFLFHMFLFAMVATAVIGCARASPRTPSIS"
BASE COUNT          397 a          745 c          823 g          667 t
ORIGIN      
        1 tcagttcaca ggtgctggcg ctcgacggct gggtggtggc gccggtgacg gtggagtttt
       61 cgagcatctc gacgccggtg ctcgccatgc tcctggtcgg ggcgggggcg ttggggttgc
      121 tgattgcccg actctgcggg aaggggctgc gcgcgcgcta ttacaagacc tggggctgcg
      181 ggctgaatct gacgcctcgc atggaataca ccgcgaccgg cttcgcgcaa ccgatcaagc
      241 aggtctttga aaccatctac cagccgacgg tgaagctgga acaggagttt ctggagcaat
      301 cgaagtactt catcaaacat cagcgtttcg agtcgcacat tgaaccggtc ttcgagaaat
      361 acctctatcg gccggtggtg acattgctgc tggcgacagc cgaccggctg cgcatcattc
      421 aggccggcag tctccatctg tatctcacct acattttcgt gacgctggtg ctgttgttgt
      481 tatgggcggg gtagggagag ccggcgggat gtgtgtacgt gtcgtgcctg gtcgttcgat
      541 cattgagtga cgggagctat gcagtcgatc atccacatcg tactggtcgt cgttcagctc
      601 gtgctgttgt tgaccctctc tccgtttgtc gtcggcctga tccgaaaggt caaagcacgt
      661 ctgcagtgcc gccgcggggc gagtctgttt cagccctacg ccgatctggc caagctgttc
      721 cggaagcagc ccgtcatctc ttcgacgacc tcctggattt ttaccgcggc gccgtacatt
      781 gtctttgcct cgaccgtagc ggcggggttg ttgatgccgg tattcgtctc tcacacaccg
      841 ctcaatttcg ccgggaacat cattgcgctc gtctatctgc tggcgttggg gacgttcttt
      901 ctgatcctgg cgggtctcga tgcaggatcg gcgttcggag ggatgggcag cagccgcgag
      961 gcgatcgtcg cgacgctggc cgagccggcc atgatgctgt ccatcctttc catcgcgttg
     1021 accgccggct ccacgaattt gagcaccatc gtgcatcagt ccgcgttgat ggaaggggtc
     1081 gtcgtggctc cgcccgcgca tttgatggcg ctggccgcct tgttcatcgt gacgctggcg
     1141 gaaaccggtc gtgtcccggt ggacaatccc gccacgcacc tggaattgac gatgatccac
     1201 gaggccatgc tgctcgagta ttccggccgc tacctggcgt tgatggaatg ggcggccggg
     1261 atcaagttgc tggtgttcct gaccttgatc gtgaatgtct ttgcaccctg gggtatcgcc
     1321 acgagtctgg cgccgtcagc catcgccgtc ggtctcctgg tgtatctcgg aaaggtggct
     1381 gcgctggctc ttgtgatcgg tgtgatcgaa tcgatgttcg cgaagctgcg attgttccgg
     1441 gtgcccgaaa tgctgggcgc ggcgttcatt ctgtcgctgc tcgcgctggt cttcttctat
     1501 acgttgaaag ggtaatgcgg tgccggcttc cacccaattg ggctctcaac tcgtcgatct
     1561 ctgctcggtc ctgctgctgc tctgttgttt tgccatcgtg gcgcagcgtc gattgtcggc
     1621 ctgtgtggat ctgtttgcgc tgcagtccgc gtttctggcg gcgactgccg cgctcgttgc
     1681 gtttctgacc ggacataagc acatttattt cgcggcggcc ctgacgatcg tcatcaaagt
     1741 gatcgtgctg cctcgcattc tccggaaggt catcgagcgt ctgaatgtct cccgtgagct
     1801 ggggatgaac atcaacattc cggcgggcct gctgatctgc ggcgcgttgg tgatcatcgc
     1861 cttctttatc acgcagccca tcattccgct cggctacctg ctgacgcgcg attctctggc
     1921 cattgcgctg gccattattt taatcggatt tttcaccatg attgcccggc agaaggcgat
     1981 cacgcagatg gtcggctttc tagtcatgga gaacggagtg ttcctgggag ccacggcggc
     2041 cgcgtacggc atgccgctta tcgttgaatt gggcgtcttc ttcgatgtgt tggtggccgg
     2101 gttgattgtc ggcatttaca cgcatcggct gcaggatacg ttcgatagcg tagacaccag
     2161 cacgctgacg gtgctgaagg aatgagtcgg ttgatgacgc atgtccggcg agtaggtgct
     2221 gtatgtggtc ggtgatcgtc ctgctgacgg ggccggtact cgccggtctg ctcagtctgg
     2281 taatccaccg cgcccgggtc ctgcacgtcg tgaacttttc gacgatgctg gcgttggccg
     2341 ttgccgagac ggcgctgacg aggcaggtgc tggccgaggg atcggtgacg acattgggcg
     2401 gctttgtcta cgtggatgcg ctctcggact ttattctgat gatcatcacg gccatcggct
     2461 tgtcctgttc gctctacatg tggtcctaca tggacgatcg ggtcgcccgg ggcatcatcg
     2521 cgccgaagcg tctcggtcac ttcttttttc tcttccatat gttcctgttt gcgatggtcg
     2581 ccacggcggt aatcggctgc gcacgcgcca gccctcgcac cccgtcgatt tc
//
LOCUS       DAORQN010000200         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1183_length_2617_cov_19.779079, whole genome shotgun sequence.
ACCESSION   DAORQN010000200 DAORQN010000000
VERSION     DAORQN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1183_length_2617_cov_19.779079"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..882)
                     /locus_tag="PK614_08600"
     CDS             complement(145..882)
                     /locus_tag="PK614_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82304.1"
                     /translation="MKGMRRGSGFVLGMLWLLIADVATAGSYVLAIGKGIEVCEAYLK
                     NLNSFPKHPPMVCERPLNHQFTDFNKPAWKELDGQEHLELLKQIWRSFDDLTVEQFEQ
                     SPLKDQWIFSLKKPEGTMGIAIQRAELDVDLDGVTELVLHYYELGTCDPLNESQFATP
                     YGSMFYVLTPDGQNIDFEKTRLMLLNGGGRPDLFLYKGRVFITHWAGNLEFKDGQLGV
                     ISKVYVAPMVPDYKNCKYRYHASGKRR"
     gene            1322..2056
                     /locus_tag="PK614_08605"
     CDS             1322..2056
                     /locus_tag="PK614_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82305.1"
                     /translation="MTLARICFTVHLLVVVLMATCFSSSASALSWPGHVGLLGEEVVP
                     CLAISPEDEHQVTDRLGTDDAYTTACRVTESIVMLWAERGGNGDSLGVLDLKRNGGPS
                     ELGRGLPWIEKTLRDKAGVLHIVAGNGTLQDGNWDAILSLYSTTTWSRIDLARRSGPD
                     SKFMVCPEPGEYNYSFTPKLVQESHRYEDRNGDGFEDIIVETVETSCLRHETKTSTAV
                     FLATMQGFKKATPAKTSRKKPVRPKS"
     gene            complement(2113..2319)
                     /locus_tag="PK614_08610"
     CDS             complement(2113..2319)
                     /locus_tag="PK614_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82306.1"
                     /translation="MRFAALVAAIVHIVRSRDIEQTKIDRFFDSARKKSLGGIPRVLG
                     YDRRQAEIEIPTKIEALTNTWEER"
BASE COUNT          619 a          703 c          650 g          645 t
ORIGIN      
        1 gtgaaaccgc ggcggcggag gcctttcagt gtgggaagac gaggatcatc ccaacgtccg
       61 cgtagcctgg actcgttaaa aaaacgaacc gcagatctca atccactttt tgacttgaag
      121 ccgtttgcca ttgtgtaatc atgcttatct cctctttcct gaagcgtgat acctgtattt
      181 acagttctta tagtcgggaa ccatcggcgc aacatatacc ttcgatatca cgccaagttg
      241 cccatcctta aattccaaat ttccggccca atgggtgata aacacccgcc ctttgtacag
      301 aaacaaatcg ggacgcccac ctccatttag caacatgagg cgagtttttt cgaagtcgat
      361 gttctgacca tccggggtga gaacatagaa cattgaacca tagggggtag caaactggga
      421 ttcattgagt ggatcgcaag tgccaagctc atagtagtgc aacactagtt ctgtaactcc
      481 gtcgagatcc acatccagtt ccgctctttg aatggcaatc cccattgttc cttcgggctt
      541 cttcaggcta aagatccatt ggtctttgag cgggctttgt tcaaattgct ccacggtgag
      601 atcatcgaaa cttctccaga tctgcttcag cagctccaaa tgctcttgtc catccaattc
      661 cttccatgcc ggcttgttaa aatcggtgaa ttgatggttc agcggcctct cacagaccat
      721 tggcgggtgc tttggaaacg aattgaggtt cttgaggtag gcttcacaca cctcaatgcc
      781 cttgccgatc gccaacacat aactccccgc cgtcgcgaca tcggcgatca acagccaaag
      841 catgccaagg acaaatcctg atcctctgcg cattcccttc atgctgccat cctttcccgt
      901 cattgcactt ttgtacggca cctcaggcga gtcacgtctt cacagcgtct tagccgatct
      961 tagctgtggg cacagtcgtc tgtggattta ggaacacaat cactgcgttc tactgcacct
     1021 ttgtgatcct gtttccacct tagtaatagg tataagtgag cgattcaata gccgtcagca
     1081 ttagggcttc acttccctct gatcctatcc cacaaggccg agaatgcctt ccaaactttc
     1141 ttttcgcgca gggggcaaga acgagctgat cgttaaggaa ttcctgcgag aaaggtcttt
     1201 tttaccttcc ccacacaagt aggaaggtag aattgtgcat tgtgaaagtc acgtgcagaa
     1261 agacgcgtcc ccaatgaatg gcccgaagga gttgagaatc aaactgaggg agtaatccaa
     1321 gatgacgcta gcacgcattt gtttcacagt gcatttgctt gtggtcgtgc ttatggccac
     1381 ctgtttctcc tcttctgcta gcgcactcag ctggccagga catgttggcc tcctcgggga
     1441 ggaagtagtt ccgtgtctcg caatctcgcc agaggatgag catcaggtta cagaccggct
     1501 aggaacagac gacgcctata ccaccgcatg tcgtgtcaca gaatcgatag tcatgctctg
     1561 ggccgaacgc ggagggaatg gtgattcatt gggtgtgctg gatctcaaga gaaatggcgg
     1621 tccgagtgaa ctcggtcgtg gactaccatg gattgagaaa actcttcggg ataaagctgg
     1681 agtgctccat atcgtggcag gaaacggaac gttacaggac ggtaattggg atgctatcct
     1741 gtccctctat tccaccacaa cctggagccg cattgattta gctagaaggt ctggaccaga
     1801 tagcaagttc atggtttgcc cggaaccagg ggagtacaac tacagcttca cgccaaagct
     1861 tgttcaagaa tcccatcgat acgaagatcg aaacggggac ggttttgagg acattattgt
     1921 tgagactgta gaaacgtctt gtttgaggca tgagaccaaa acctctaccg cagtattcct
     1981 cgcgacgatg cagggcttta agaaggcaac gcctgcgaag acttcccgga agaagccagt
     2041 ccgccctaag tcgtaaatat gaagccctgg cagagtccga tgattcacca cgagctatgg
     2101 acctactcat gactagcgct cttcccaagt attagtaagc gcttcaatct tcgtcggaat
     2161 ctcgatttcg gcctgcctcc gatcgtatcc cagaacgcgg ggaatgcctc ccaaactttt
     2221 ttttcgcgca ctgtcaaaaa accggtcgat ttttgtttgc tcgatgtccc ggcttcgaac
     2281 gatgtgcaca attgccgcca ctagcgcagc aaagcgcaca tacgcctctt tatgcctact
     2341 tccttttgag tcgtcgctgt ctgaaaatct gtcctctcct gtcccctcgt gacccctagt
     2401 ggcctcttcc ggggcctttg cttcggtccg gtgattcgcc tagggtgaga cgaacgggaa
     2461 cagagctcaa taggagagaa cgatgtccga ttcggggcgc gtcacacagc aacactgtca
     2521 tccgtcgccg ggagacaaag ccggtccgcg ccgcatgccg ggaggctgcg gggcggagcg
     2581 gaatggcccc cgtcttggta acacgggggc gctaccc
//
LOCUS       DAORQN010000201         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1185_length_2616_cov_4.852401, whole genome shotgun sequence.
ACCESSION   DAORQN010000201 DAORQN010000000
VERSION     DAORQN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1185_length_2616_cov_4.852401"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..440)
                     /locus_tag="PK614_08615"
     CDS             complement(195..440)
                     /locus_tag="PK614_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRB82307.1"
                     /translation="MALTLYHVQWCPDCAVVRDRLDELKLSYEDVVVPDFRPMRTQVF
                     EVSGQYYVPVLKDGDTVLTETHDILAHLHTQYDKARP"
     gene            complement(516..1610)
                     /locus_tag="PK614_08620"
     CDS             complement(516..1610)
                     /locus_tag="PK614_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82308.1"
                     /translation="MVITELDSVPGGIGLTGCLTRAYDDQFTIERQPPGLPPERSPLI
                     AGRDGMLQGFAAMLRAQQGDQDGCLAIVVSDEAKEYRAEMEWMAQRLREIGYQAFCVE
                     PRAVHFSEEGLFLQEETGPRPIGLLYRFFELFDLKNIPKAELVMYAAKKGQVTVTPPY
                     KPALEEKLAFGLFHHPELAPFWEQALRPETAINLRRLLPRTWILDPQPVPPSAVIPHL
                     TISGRAVSDFRRLAQTTQKERQLVLKPSGFSELAWGSRGVSIGHDLPQVEWATALEQA
                     LQAFPTTPYVLQEFHKGRLFELSYFDERSDTVVPMSGRVRLSPYYFVVGDKAELGGIL
                     ATLCPANKKIIHGMVDAIMAPCAIAPNLAS"
     gene            complement(1628..2020)
                     /locus_tag="PK614_08625"
     CDS             complement(1628..2020)
                     /locus_tag="PK614_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82309.1"
                     /translation="MALAQHSRDPLNSDEARRRCLAIRDVLVRAGVYGPAETGDGAAT
                     TDSSIPTPRFRLAPTPFPLSSSDVTFFQELGQDLLSFYRALNRLYQDSTRGTQPAWVA
                     AYLDQGKPESLITYSRMNRFRNLIPDPT"
     assembly_gap    2394..2403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2404..>2616
                     /locus_tag="PK614_08630"
     CDS             <2404..>2616
                     /locus_tag="PK614_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB82310.1"
                     /translation="EIGTAIAAGLAPALFPNPDGSQIDLAPNPEGDGYVCPAFDCATS
                     RCRIYEVRPLDCRLYPFALMWDARHE"
BASE COUNT          511 a          745 c          827 g          523 t
ORIGIN      
        1 ctgggcggcc ttttggaacg gcctgtaggt gatgcgtatg gaaatacgtg atcgcagccg
       61 gcgctgaaaa gcacacgggc gacgactgtt tgttaaaaat ttgctaagtg gccgagccct
      121 gcaggccggc gcctgcccga gggatcgggg ccggtccgtg atcggcgctc gcgctggaga
      181 tccgtgcgag gtcctcacgg ccgtgccttg tcgtactgtg tgtgcaggtg agcaagaatg
      241 tcgtgggttt ccgtcagaac ggtgtctccg tctttcagga ccgggacata gtattggccc
      301 gacacctcga agacttgtgt gcgcatcgga cgaaagtccg gaaccaccac gtcttcgtaa
      361 gagagtttca gttcgtccag acggtcccga acgaccgcac aatccgggca ccattggaca
      421 tgatagagcg tcagggccat gagtacctat ggggaaaaag gttgagtagg catctgcaaa
      481 tgaaccggtg ctgctgcagc gatcatcagg tcaccttatg acgcgaggtt tggcgcgatg
      541 gcacagggcg ccatgatcgc gtcgaccata ccgtggatga ttttcttgtt ggcagggcag
      601 agggtcgcga gaatcccgcc gagttcggcc ttatcgccga cgacgaaata atagggtgac
      661 aggcggactc tcccggacat cgggaccacc gtgtcgctcc gctcgtcgaa ataggacaac
      721 tcgaacaggc ggcccttgtg gaattcctga aggacatagg gcgtcgtcgg gaacgcctgg
      781 agcgcctgct ccaatgccgt ggcccactcg acctgcggca ggtcgtgccc gatggacacg
      841 ccacggctgc cccaggccag ctcagagaat cctgatggtt tgagcaccaa ctggcgttcc
      901 ttttgggtgg tctgggctaa gcgccggaag tccgacaccg cgcgaccgct gatcgtgagg
      961 tgcgggatga ccgcggaagg aggtaccggt tgtgggtcga ggatccaggt tctcggcagc
     1021 aatcgtcgca agttgatggc ggtttcggga cgtaatgcct gttcccaaaa cggagccagt
     1081 tccggatggt gaaacaggcc gaacgccagc ttttcttcaa gcgcgggttt gtagggcggc
     1141 gtgaccgtca cctgcccctt cttggcggca tacatcacga gttcagcctt ggggatgttc
     1201 ttcaagtcga acagttcgaa aaagcggtac agcaaaccga tcggccgtgg tccggtttct
     1261 tcctgaagaa acaggccctc ttccgagaaa tgaaccgccc gtggttcgac acagaaggcc
     1321 tggtacccga tctcacgcaa tcgttgggcc atccattcca tctctgcccg gtattccttg
     1381 gcctcatcgg agacgacaat cgcgaggcac ccgtcctggt cgccttgctg ggcgcgcagc
     1441 atggcggcga atccttgtaa catcccgtca cgccccgcga tcagaggaga acgctcaggc
     1501 ggcagaccgg ggggctggcg ctcgatggtg aactgatcat cataggcgcg agtcaggcaa
     1561 ccggtcaggc cgatcccgcc cggtaccgaa tcgagctctg tgatcaccat cccatcggcg
     1621 gttggaatca cgtcgggtcg ggaatgaggt tgcggaaacg gttcatccgg ctataggtga
     1681 tcagcgattc cggcttgccc tggtcgagat aggccgccac ccaggcgggc tgagttccgc
     1741 gggtgctgtc ctgatacaga cggttcagag cccgatagaa ggagaggagg tcctgtccca
     1801 gctcctgaaa aaacgtgacg tccgaggagg agagcggaaa tggcgttggc gccaggcgaa
     1861 accgtggcgt cggaattgag gagtcggtcg ttgccgcgcc gtcgccggtt tcggccgggc
     1921 cgtagacccc ggcgcgaacg agaacatcac gaattgcgag acaacgtcgg cgggcttcgt
     1981 cggagttcaa tgggtcacgt gaatgttgtg ccagtgccaa tgcaatatcc tcgagactcg
     2041 cagcgcagtc gcatgcctcg cactcgcgtt tggattcgag gtgagcaaat cgtaccatgg
     2101 cctctgacac cggtacaagt cagcggagcg ccggagctgt tcgaagaacg actcgttacc
     2161 gccccgtgag agcgcggcaa cgcgggggtg aaacgtttcg aatagtagga cgctccactt
     2221 gttagtgcta caatgcgccc acagctcatg acgagttcat tcgtgccaat tgtcccgaca
     2281 aatccttctc gtcccggggg tgatgctctc ctgcagatcg tgccgagttc cgcctgcctc
     2341 agttgcgatg tctgttgccg gtttccggag cgggacagtt tcctgcggcc cttnnnnnnn
     2401 nnnacgaaat cgggaccgca atcgcggccg ggcttgcccc tgcgttgttc ccaaaccccg
     2461 atggctcaca aatcgatctg gcgccgaatc cggaagggga cgggtacgtc tgtccggcct
     2521 tcgattgcgc aacctcgcgg tgtcgcatct atgaggtccg tcccctcgat tgccggctct
     2581 acccgtttgc cttgatgtgg gatgccaggc atgaac
//
LOCUS       DAORQN010000202         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1188_length_2606_cov_7.793414, whole genome shotgun sequence.
ACCESSION   DAORQN010000202 DAORQN010000000
VERSION     DAORQN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_2606_cov_7.793414"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..1160)
                     /locus_tag="PK614_08635"
     CDS             complement(315..1160)
                     /locus_tag="PK614_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HRB82311.1"
                     /translation="MMISQDGAFDRGSVAVVVPAYNRAHFTRDEEVSFRHLEHVLGRY
                     DKFLAVPRSLKIERPGYQIQRFDDGYFGSAIANGKLMLSPAFYESFRKYRYILIYQLD
                     ALVFSDQLLEWCATDVDYIGAPWVQCADSPWVGTPRVGNGGLSLRKVSSFLRVLSSEQ
                     YWIHPDAYWQRVVSGTPWYLRGVYLPRKWFKYIKRFNGVKRQVAQWHLRPDGTKNEDH
                     FWSDEAVRYDDQFKVASFDMGLRFAFEVVPRHCFELNNRRLPFGCHAWPRYDRAFWEP
                     YLLKS"
     gene            complement(1346..2452)
                     /locus_tag="PK614_08640"
     CDS             complement(1346..2452)
                     /locus_tag="PK614_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB82312.1"
                     /translation="MTKLRVGVIGAGAFAESCHVPGLQSHPEAEVVVLCGRDSARTFS
                     MAQRLGVPEVSLDYEEVCARTDIDAVTIATPNVVHARQAQAAFAAGKHVFCEKPLGMN
                     TEEAADMLRAAEQSHKIHQTAFTYRYLYGVQELKRRLLNGEIGEPYYVAVHYDSWDGL
                     RPGSRIGFREHLDSAGAGVLYDVGSHLFDLVGFVLGPIEAVSGNTILVPRKRIDARTG
                     ALAHVETDDIASAAFTCGGGIQGQLFASRATPNSGDKAYIELVGRQGALKASLSRGSV
                     DVLTISRPSQPAWEVVPLPVQASDGQPHCLSRMMHSFVEACLRGSLNGEIDASFHDGL
                     AVQRAMDAVRQSSAEPTAISLDADFNPTAFGGVA"
BASE COUNT          596 a          821 c          702 g          487 t
ORIGIN      
        1 gcatcctttg aatcgtctcc tcagaactcc gcacgaataa ccgattcgcg agtcggtttt
       61 ctccaaatct cccgtaagat acgcccatga ctcactgagg taaaccccgg caatcggccc
      121 tagtacggcc ctactccatg gagcagtgat cagaagaggt tgaaagagag accgtcacgt
      181 tcacggcgga caggcacgca atcgcatcat gtgagacacg gagaagggag gagggctatg
      241 agtcgaggga ctcctgaatg tggccccgaa tcacggaacg ggcttgcctg acagaccgat
      301 gcagcgggac ggaactacga cttgaggaga tagggctccc agaaggcgcg atcataccgc
      361 ggccaggcat ggcaaccgaa aggtagtcgg cgattgttga gctcaaagca atgcctggga
      421 acaacctcga aggcgaaccg aagccccatg tcgaacgagg cgaccttaaa ctgatcgtca
      481 tatcgcaccg cctcgtcgga ccagaaatga tcttcatttt tggttccatc gggacgtaga
      541 tgccattgcg cgacttgtcg cttcacgcca ttgaatcgtt tgatatattt gaaccacttc
      601 cttgggagat acacacctcg caggtaccag ggcgtcccgg agacgactcg ctgccaatac
      661 gcgtcagggt ggatccaata ctgttccgac gacagcaccc gtaggaagct tgagactttt
      721 cggagagaca acccgccgtt tccgacacgc ggcgtcccca cccaggggct gtcggcacat
      781 tgcacccacg gtgcgccgat ataatcgaca tcagtggcgc accattccaa caactgatcc
      841 gaaaacacca gggcatcgag ctgataaatg agaatataac gatacttccg gaacgactcg
      901 taaaaggcag gcgacagcat cagtttccca tttgcaatcg cactcccgaa atagccgtcg
      961 tcgaaccgtt gaatctggta gcccggccgc tcaatcttca gactccgcgg caccgccaga
     1021 aatttgtcgt accggccgag aacatgctcc agatggcgaa acgagacttc ttcgtcccgg
     1081 gtaaagtgtg ctctgttgta agcaggcacc accaccgcga cgcttcctct atcgaacgca
     1141 ccgtcctgcg atatcatcat tccggcctcg cctcgcctgc tcggtagtat cagcctcacc
     1201 cgccccgatc acaccgtgca gctcctcgtg gccttgctcc gtcccttcca ggaccaccgg
     1261 gcaactgatg catgcgcact gacaggcagc accatgcccg agccaggctg cgcgaaggcc
     1321 cgcaccaagt gaatgaacgg tcaggttatg ccactccgcc gaaggccgta ggattgaaat
     1381 cggcgtccag ggagatcgca gtgggttcgg ctgaggattg tcgcaccgca tccatggcgc
     1441 gctgcacggc gagtccgtca tgaaaggacg catcgatctc cccattcaaa cttccgcgaa
     1501 gacaggcctc gacaaaactg tgcatcatgc gggaaagaca atgaggttgg ccatccgagg
     1561 cctggaccgg caacggcacg acttcccagg ccggctgcga cgggcgcgag attgtcaaga
     1621 catccaccga cccccggctc aatgaagcct tcagggctcc ctgtcgcccg accaactcga
     1681 tgtacgcttt atcgccggaa ttcggggtcg cgcgactcgc aaacaactgc ccctgaatcc
     1741 cacctccaca ggtaaacgcc gccgatgcga tgtcgtcggt ttctacatga gcgagcgcgc
     1801 cggttctcgc atcgatccgt ttccgtggaa ccagaatggt atttcctgag acggcctcga
     1861 tcggcccgag cacaaatccg acaaggtcaa agaggtggga cccgacgtcg tacaacaccc
     1921 cggctccggc ggaatcgaga tgttcgcgaa agccaatcct cgaaccaggt ctcaggccgt
     1981 cccaggaatc atagtgcacc gccacataat acggctcgcc gatctccccg ttcaggagtc
     2041 ggcgcttcag ttcctgcaca ccgtacagat agcgatacgt gaacgcggtc tggtgaatct
     2101 tgtggctttg ctccgcggcc cgaagcatgt cagccgcttc ctccgtgttc atcccgaggg
     2161 gtttttcaca gaacacgtgc ttcccggcag cgaacgcggc ctgagcctga cgggcgtgga
     2221 ccacattcgg ggttgcaatc gtgaccgcat cgatgtcagt ccgagcgcag acctcctcat
     2281 agtcgagcga cacctccggc acacccaacc gctgcgccat cgaaaaggtg cgtgccgagt
     2341 ctcgcccgca gaggacgaca acctccgctt ccggatgcga ctgcaagccg ggcacatgac
     2401 agctttcggc aaacgctccg gccccgatta ctcccactct taatttggtc atccggccgc
     2461 tctgcaaaaa atgtcgcgtc tgacgcaacg gaaagggatt ccctcgcgtc agccccccga
     2521 caacagtgaa ttgtatggtt acacaggatg ggagccatgc aggcatctag gctcgctcgt
     2581 tcactctagc cgattaccgg aggagt
//
LOCUS       DAORQN010000203         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1189_length_2606_cov_5.352411, whole genome shotgun sequence.
ACCESSION   DAORQN010000203 DAORQN010000000
VERSION     DAORQN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_2606_cov_5.352411"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..>260
                     /locus_tag="PK614_08645"
     CDS             67..>260
                     /locus_tag="PK614_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB82313.1"
                     /translation="MTRQTRTIFGLALGALFTALILATAPVQAAETRDLILAAYSVPK
                     EAYERHIIPAFQRHWKQKTG"
     assembly_gap    261..282
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <283..1141
                     /locus_tag="PK614_08650"
     CDS             <283..1141
                     /locus_tag="PK614_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB82314.1"
                     /translation="VRVRSSYGASGAQARAIIGGFDADVAVLSLEGDVDQIVKAGLIT
                     HDWRKAPHGGMISASVVALGVRKGNPKGVKGWDDAARPGVEVLYPNPKTSGGAMWDVI
                     AIYGAGLKLAEQRAGGKPLAPEAAASQAVDLLTRVQRNVKVMDKSGRESVTTFERGVG
                     DVIVTYENELLPRVKSGRPYEIIVPAETVWIENPAAVVDTYADRHKARDLAEAFVAFL
                     HGPEAQAAFVELGFRPLDKNAGTQATTLPLPARLFTIADLGGWDQVSNKLFGAQGLWT
                     KIVEDVTKK"
     gene            1244..2125
                     /gene="cysT"
                     /locus_tag="PK614_08655"
     CDS             1244..2125
                     /gene="cysT"
                     /locus_tag="PK614_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRB82315.1"
                     /translation="MTAHLLLRLALRSAAIGYVLVLIFLPLAALAHQSFAAGWDRFIQ
                     DLSAPQAAAALWLTVETAAIATAINALFGTLSALVLVRYEFPGRWLLNALVDLPFAIP
                     TLVAGLMIAAIYGPTSLLGTWLHQGGMAVLYNQPGIILAMVFVTMPFTIRSLQPVLMG
                     LERDQEEAAFTLGASPWITFWKVTVPSILPGLLTGVFLTFVRALGEFGSIVIVAGNIP
                     MKTQVASVYVYGEIESYNPQAATSVSVLILLISFLVLLLLERLTRRPGERLPRLFQWT
                     RQKDERGSESAVSTATI"
     gene            2174..>2606
                     /locus_tag="PK614_08660"
     CDS             2174..>2606
                     /locus_tag="PK614_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter"
                     /protein_id="HRB82316.1"
                     /translation="MRWLLIGIVWLYFLVLLVGPILYMASQSFSEGLAAFWAEISRPE
                     ALHGFTLTAEITLIVLVLNVIFGTMTAMVLARQKFWGRSLVSGVIDLPFAVSPVIAGF
                     MLILLFGPDTVLGTFFGQAQIKVLFALPAMILATLFVTFPFM"
BASE COUNT          484 a          787 c          770 g          543 t
ORIGIN      
        1 ttctcgacca ttacacgctc agtcgattcg cccattcgaa ataggcagaa ggagccgcga
       61 cccgctatga ctcgtcagac acgtaccatt tttggccttg cactaggcgc cttattcacc
      121 gcgctcatac tggcaactgc accggtacag gccgccgaaa cccgtgatct catcctggcc
      181 gcctacagcg tgccgaaaga agcctacgaa cgacacatca tccccgcctt tcaacgacat
      241 tggaaacaaa aaaccgggca nnnnnnnnnn nnnnnnnnnn nncgtgcgtg tgcgcagctc
      301 ttatggagcc tcgggcgctc aggcgagggc tatcatcgga ggtttcgatg ccgatgtcgc
      361 cgtgctctcg ctggaaggcg acgtcgatca gatcgtcaaa gccggattga ttacccatga
      421 ttggagaaag gcaccccatg ggggcatgat ctccgcatcg gtcgtggccc tcggcgtacg
      481 caagggcaat cctaaaggcg ttaagggatg ggacgatgcc gcgcgccccg gcgtggaagt
      541 gctctacccg aatcccaaga catccggcgg cgcgatgtgg gatgtgatcg cgatttatgg
      601 cgccgggttg aaactggcgg agcagcgcgc aggtggaaag cccctcgccc cggaggcggc
      661 ggcttcgcag gccgtggact tactgacccg cgtccaacgc aacgtcaagg tcatggacaa
      721 gagcggccgc gaatcggtga ccacgttcga acgcggcgtc ggcgatgtca tcgtcaccta
      781 cgaaaacgag ttgctgccgc gcgtgaagag cggacggccc tatgaaatca tcgtgccggc
      841 cgaaaccgtc tggatcgaaa acccggcggc ggtcgtcgac acctatgccg accgccacaa
      901 ggccagggat ctcgctgaag cgttcgtcgc gtttctccac ggaccggagg cacaggctgc
      961 cttcgttgaa ttgggctttc gcccgttgga caagaatgcc ggtacccagg ccaccacgct
     1021 cccactaccg gctcgactct tcacgattgc agatctcggc ggctgggacc aggtgagcaa
     1081 caaactattc ggcgcgcaag gactgtggac gaagatcgtc gaagacgtga caaagaaata
     1141 gcagcctcct gagaaaatga ttcgtgcaga atcatcacgg ccaacgatag gaatggtgcc
     1201 tgtcgaacat gcgtactcct aaccagcaac gtagagcgct cgtatgaccg ctcatctctt
     1261 gctccgcctg gcacttcgat ctgcggcaat cggctatgtg ctggtcctga tcttcctccc
     1321 cctcgcggcg ctggcgcatc aatcgttcgc agccggatgg gaccggttca tccaggatct
     1381 gtcggctccc caagctgcgg cagcgctgtg gctcaccgtg gaaaccgccg ccattgcgac
     1441 cgccatcaat gccctgttcg gcaccttgtc ggcgcttgtg ctggtgcgct acgaattccc
     1501 gggccggtgg ctcctcaacg cgctcgttga tttgcccttt gccattccga ccctggttgc
     1561 cggcctcatg atcgccgcga tctatggccc gaccagtctg ctgggcacct ggcttcacca
     1621 gggcgggatg gcggtactct acaaccagcc cggcatcatc ctggctatgg tgtttgtgac
     1681 gatgccgttt accatccgct ctctgcagcc ggtgttgatg ggacttgagc gcgatcagga
     1741 agaagcagcc ttcacgttgg gagccagccc ctggatcacc ttctggaagg tcacggtccc
     1801 gtcgatccta cccggcctgc tgacgggcgt cttcctcacc ttcgtgcggg cgctcggcga
     1861 attcgggtcc atcgtgatcg tggcgggcaa tattccgatg aagacgcagg tggcttccgt
     1921 ctatgtctat ggggaaatcg agagctacaa cccccaggca gccacctccg tctcggtgct
     1981 gattctactg atctcctttc tggttctctt gctcttggaa cggttgaccc gtcgtcccgg
     2041 agaacggctg ccaagattgt ttcaatggac ccggcagaag gatgagcgcg gctctgagtc
     2101 agcggtgtcg accgcgacaa tctgagacta tgaatccggc agagcgtgaa ttggttgtga
     2161 tgggagtgtg ggcatgcgct ggctgttgat cgggatcgtc tggctctact tcctcgtcct
     2221 gctggtggga ccgatcctct atatggccag ccagagcttc agcgaggggc tggcggcatt
     2281 ctgggccgag atctcgaggc cggaagcgtt gcacggcttc acgctcacgg cagaaatcac
     2341 cctgatcgtg ctggtgctga acgtgatctt cggcaccatg acggcgatgg tgctggcgag
     2401 gcagaagttt tggggccgca gcctggtgag cggtgtcatc gacctgccgt ttgcggtctc
     2461 ccccgtcatc gcgggcttca tgctcatatt gctattcggc ccggacaccg tgctaggcac
     2521 gtttttcggc caggcacaga tcaaggtgct gtttgccctg ccggccatga ttctcgcgac
     2581 cctgttcgtc acctttccat ttatgg
//
LOCUS       DAORQN010000204         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1194_length_2597_cov_4.839890, whole genome shotgun sequence.
ACCESSION   DAORQN010000204 DAORQN010000000
VERSION     DAORQN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_2597_cov_4.839890"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..784)
                     /gene="bluB"
                     /locus_tag="PK614_08665"
     CDS             complement(8..784)
                     /gene="bluB"
                     /locus_tag="PK614_08665"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HRB82317.1"
                     /translation="MARDKRERLTRTTAEPSEPFSKAEREAVYRAIFERRDVRRNFLP
                     TPIPDEVLVRLLNAAHHAGSVGFMQPWDFVVVRTDETKRAVKQLFMDANAAAALRYKT
                     PRADLYRTLKLEGIEESSLNLCVTCSRQRGGPHVLGRSTVRDTDLYSTCCAIQNLWLA
                     ARAEGIGVGWVSILNHAALKRVLGIPKSVKVLAYLCLGYVSEFAKKPDLEAAGWRARL
                     SVQDLVHYEAWGKRVEGKERLPGCESPRSIQERATRGKRG"
     gene            complement(986..1585)
                     /gene="cobO"
                     /locus_tag="PK614_08670"
     CDS             complement(986..1585)
                     /gene="cobO"
                     /locus_tag="PK614_08670"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRB82318.1"
                     /translation="MAEQDDHTAKMQRLKASVDRRIEAAQDEKGLLIVYTGAGKGKTT
                     AALGMALRCLGHGMKVAIVQFIKGAIDTAEERILKSFGDRVTFLRMGEGYTWETQDRE
                     RDTSHAQAAWAKACGFMRDPSYAMVILDEFNIALHHGYVAVTEVLPRLQQRPAMQHVV
                     ITGRGAPEELIEAADLVTEMKQVKHPFRKGIKAQAGVEL"
     gene            complement(1587..>2597)
                     /locus_tag="PK614_08675"
     CDS             complement(1587..>2597)
                     /locus_tag="PK614_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HRB82319.1"
                     /translation="AAAMASGYAKFDAAVQVCGVLFNRVRSEGHYRLLREAVEAETDL
                     TVVGYLQPDASVTIQDRHLGLRTAIEQGQGDLYDRLARAAAQTIDLDRVEAMARSAGE
                     CPGEDLPITRPTGTQQPVRVGVAYDAAFCFYYQDNLELLESAGAEIVRFSPLHDHELP
                     VADLLYFGGGYPELYGETLASNMSMRSAVRTFARGGGAVYAECGGLMYLMEAIRDGEG
                     RRHEMVGVFPADAVMRKNGMTLGYRRVEVAESCILGGAGTVVRGHEFHYSMLEPRGDL
                     HYACTLSDAAGQPVGQDGLTRGNTLALYSHLHFGSHPDVVAELLGSARRLRGAQASAM
                     KG"
BASE COUNT          518 a          915 c          636 g          528 t
ORIGIN      
        1 ccgccggcta accgcgtttg ccccgcgtcg cccgttcttg tatagacctt ggtgattcgc
       61 atcccggtag tctctccttc ccttcgactc tcttacccca tgcctcatag tgcaccaaat
      121 cttgcacaga aagcctggct cgccacccgg cggcttcaag gtccggcttc ttcgcaaact
      181 ccgacacata tccgagacag aggtaggcca gcaccttgac cgactttgga atacccaaca
      241 cgcgcttcag cgccgcgtga ttcaagatac tgacccaccc aaccccgatc ccttcggcgc
      301 gtgccgccag ccagagattc tgaatggcac agcaggtgct gtagagatct gtatcccgca
      361 cggtggaacg gcctaacacg tgcgggccgc ctcgttggcg gctgcaggtc acacagagat
      421 tgagcgacga ttcttcaata ccttccagct tgagcgttcg atacagatcc gcacgaggtg
      481 ttttgtaccg gagcgccgca gccgcattcg cgtccataaa cagctgcttc accgcacgct
      541 ttgtctcatc tgtacggacc accacaaagt cccagggctg catgaaccct accgatccgg
      601 catgatgcgc ggcgttcagt aaccgcacga gcacttcgtc gggaatcggc gtcggcagaa
      661 agtttctgcg cacatcccgg cgctcgaaaa tagcccgata gaccgcctct cgttcagcct
      721 tggaaaacgg ttctgaaggc tcggccgttg ttcgagtcaa ccgctctcgt ttgtcacgtg
      781 ccatgaggca tttcattcga agactgcggt ccaagtactc catccgccac ctgctgcaaa
      841 cagacggggc aaaggcagtc cttaaaacgt gccgcaatcc aatccatctg ctcctcggta
      901 ataccgatct tcccgcacca gcattgatac ccgacacact cgaacgtctt ccggcaatgc
      961 tcacaggtct tgctcaccgg ccccctcaca actccacccc cgcctgcgcc ttgatgcctt
     1021 ttcggaacgg atgtttcacc tgcttcatct ccgttaccag atctgcagcc tcgatcaatt
     1081 cctccggagc ccctctgccg gtgatcacga catgttgcat cgccggccgc tgctgcaaac
     1141 gcggcagaac ttcagtcacc gccacatagc cgtggtgcag tgcaatattg aattcatcca
     1201 ggatcaccat cgcataagac ggatcacgca tgaacccgca ggccttcgcc caggccgctt
     1261 gcgcatggct cgtatcccgc tcccgatcct gcgtctccca ggtataccct tcgcccatac
     1321 ggaggaacgt cacgcgatcg ccgaacgatt tcaggatgcg ctcctctgcc gtgtcgatcg
     1381 cccccttgat gaattgcacg atcgccacct tcatgccgtg gccgaggcaa cggagggcca
     1441 tcccgagcgc ggcggtcgtt tttccctttc ccgcgccggt gtagacgatc agcagcccct
     1501 tctcgtcctg ggccgcttcg atccgccgat ccacggaggc cttcaagcgt tgcatcttcg
     1561 ccgtatggtc gtcctgttcc gccatctcat cctttcatgg cagaggcctg cgccccgcgt
     1621 aatcgacggg cagaccccag caactcggcc acgacatccg gatggctgcc gaagtgcaga
     1681 tggctataca acgcgagggt atttcccctc gtcaacccgt cctggccgac cggctgaccc
     1741 gccgcatccg acaaggtgca ggcatagtgc aggtcgcctc ttggttccag catggaataa
     1801 tgaaactcat gtcctcgcac cacggtcccg gctccgccca agatgcagga ttcagcgacc
     1861 tccactcttc gataacccaa ggtcatcccg tttttccgca tcaccgcgtc cgccggaaac
     1921 accccgacca tttcatgccg cctgccttcc ccgtcacgaa tggcttccat caaatacatc
     1981 agcccgccac actcggcata gacggctccc ccgccccggg caaaggtccg cacggccgaa
     2041 cgcatggaca tgttggacgc caatgtctcg ccatacagct cggggtaacc tcccccgaaa
     2101 tacagcagat ctgcgaccgg caactcgtga tcgtgcaagg gcgaaaacct tacgatctcc
     2161 gcgccggccg attcaagtaa ctccagatta tcctgatagt aaaaacaaaa ggccgcatcg
     2221 taggccacgc cgactcgaac cggttgttgt gttcccgtcg gcctcgtgat cgggagatct
     2281 tctcccggac attccccggc cgatcgcgcc atggcctcga cccgatccaa atcgatcgtc
     2341 tgtgcagcgg ctctcgccaa tcggtcatac agatcgccct gcccctgctc gatggcagtc
     2401 ctcagcccga gatggcgatc ctggatcgtc accgaggcat cgggctgcaa gtatcccacg
     2461 acagtcaggt ccgtttccgc ctccaccgcc tctcgcaaca atcggtagtg accctcactc
     2521 cgcacgcgat tgaacagcac tccgcaaacc tgtacggccg cgtcgaattt cgcatacccg
     2581 gatgccatgg ctgcggc
//
LOCUS       DAORQN010000205         2581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1200_length_2581_cov_7.903009, whole genome shotgun sequence.
ACCESSION   DAORQN010000205 DAORQN010000000
VERSION     DAORQN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_2581_cov_7.903009"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PK614_08680"
     CDS             complement(<1..441)
                     /locus_tag="PK614_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB82320.1"
                     /translation="MSLLDQFFVYHPEPWKEGDWSATGGVPLEDVWFQAADGTKLFGW
                     YAEQSAASPVLLWCHGNAGNMVHRLDNLRALYRLGLSVFLFDYRGYGRSQGRPSENGL
                     YQDAIGAYDYLTRVCRIRPERLVIFGRSLGGAVAGELATQRPAMG"
     gene            complement(448..1080)
                     /locus_tag="PK614_08685"
     CDS             complement(448..1080)
                     /locus_tag="PK614_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB82321.1"
                     /translation="MWLPIFLCFLLGSLWHPLAAGSAEFGEIDLGGYLLGSWPRDQTL
                     FNQGDTVPASVQHGFGAGLKIGLFPHATRRMLGIEIDSYGHGGALSFPNTANGHNNGT
                     GRSSLLVLNTMVNLVLRYPGEVVTPYIGIGGGWSHGTLLNPNIIGRADKDFESARAFG
                     HQYLAGAQVMVSPKVYVFGEYRYFSANYHWDGLAVDFRVHYGVVGVGLRF"
     gene            complement(1185..1511)
                     /locus_tag="PK614_08690"
     CDS             complement(1185..1511)
                     /locus_tag="PK614_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HRB82322.1"
                     /translation="MKKVTVLFSLSLISAAYLSLTGCQIVASPMAGGIYNETKYGDVA
                     TTHNSATKEGKACGTSILGWVATGDASVSAAKAAGGITMVASVDHSAKNILGILGEWC
                     TIVKGS"
     gene            complement(1702..2424)
                     /locus_tag="PK614_08695"
     CDS             complement(1702..2424)
                     /locus_tag="PK614_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB82323.1"
                     /translation="MKKVWLLIVMGLMCSSEFLGPQVVAAQEPPSADIPTKEPRKTEA
                     YFAPYLLGTFPIDKNLSIGGNGFSNETFRGTNIQGSGGAGLKAGVYPGAMGGVLGLEG
                     EFFGHGGKLKTSGGAFPSADTNLLVLNWMVNILARYPGEILQPYVGAGAGASLAHLRD
                     INFQTNSGALTGKAGDVAFAYQFLAGMRAYVNKKVYVFGEYKYFGSAYDWKSESSSGV
                     SGPTTSLTFRTHIVALGLGVTF"
BASE COUNT          624 a          796 c          668 g          493 t
ORIGIN      
        1 ccccatggca ggccgctgcg tcgccagttc cccggccacc gcgcctccca gcgagcgacc
       61 gaaaatcacc agacgttccg gcctgattcg acaaacgcgc gtgagatagt cataggcccc
      121 gatcgcatcc tgatacagcc cgttttccga cggccgcccc tggctcctcc catatcctcg
      181 ataatcgaag agaaacaccg agagccccaa ccgatatagg gcacggagat tgtccaaccg
      241 atggaccata ttgccggcat tgccgtgaca ccacagcagg accggagacg cagcagactg
      301 ctccgcatac caaccgaaca gtttcgtccc atcggcagcc tgaaaccaga catcctccaa
      361 cggcacgccg ccggtcgcgg accaatcgcc ctctttccac ggctccggat gatagacaaa
      421 gaattgatcg agcagactca tggcagctta gaaccgcagc ccaaccccga ccactccata
      481 atgaacgcga aaatctaccg cgaggccgtc ccaatggtag ttggcagaga agtagcgata
      541 ctctccgaag acatagacct tcgggctgac catgacctga gcccccgcca gatactgatg
      601 cccgaatgcg cgcgcggact cgaaatcctt atcggcccgc ccgatgatat tgggattgag
      661 caacgtccca tgcgaccagc cgccaccgat cccgatgtag ggcgtcacaa cctcccccgg
      721 ataccggagc accaggttga ccatggtatt cagcaccagc aagcttgagc gcccggtccc
      781 attgttgtgt ccgttcgccg tattcgggaa ggaaagtgcg ccgccatgcc cgtaggaatc
      841 aatctcgata ccgagcatcc ggcgcgtggc atgtgggaaa aggccgattt tcaaccccgc
      901 gccgaagcca tgctgaactg aagccggcac cgtatcgccc tggttgaaga gggtctggtc
      961 cctcggccaa ctccccagca aatacccgcc cagatcgatt tcgccgaatt ccgcgctgcc
     1021 ggctgcgagc ggatgccaga gggaacctaa gagaaaacac aggaagatgg gaagccacat
     1081 acacgtcgta ccgtaccatg taggaggcca ctgaacgaag aaagaaaaaa gggtcttcgg
     1141 gatactctcc cgaagaccct ttctcagagg gataactaac cggatcagct gcccttaacg
     1201 atcgtgcacc attcgccaag gatgccgaga atgttcttcg ccgagtgatc aaccgacgcc
     1261 accatggtga tcccgccggc tgctttcgcc gccgagacgc tggcatcacc cgttgctacc
     1321 cagcccagaa tcgaggtacc gcaggcctta ccttccttcg tggcgctgtt gtgggtcgtg
     1381 gccacatcgc cgtacttcgt ttcgttgtag attccacccg ccatcggaga cgccacgatc
     1441 tgacaaccgg tgaggctgag atacgctgcg ctgatcaggc tcagagaaaa caacacggtc
     1501 accttcttca taagtcctcc ctctcgaggt tagcggttga tggtgcccac caagttgggg
     1561 gctctacgtg gcgggagtat aggtaacctc tccaggcttg tcaatcgcaa agccggttca
     1621 cgcgcaagag ccgtcgaaca gggccgtaca aaccctaggg ccttacggcc ccactgcgga
     1681 aagggccgtt gatggatggc ctcaaaaggt gacgccaagc ccgagcgcga caatgtgggt
     1741 tcgaaaggtc agtgatgtgg ttgggcccga cactccagag gaactctcgc tcttccagtc
     1801 atatgcagat ccgaagtatt tgtactcacc gaatacatac actttcttat tcacatacgc
     1861 gcgcatgccg gcgagaaact gataggcaaa ggccacgtca ccggcttttc cagtcaatgc
     1921 cccggagtta gtctgaaagt tgatatcacg aaggtgagct aacgaggcac cggcgccggc
     1981 accgacgtaa ggctgaagaa tctcaccggg gtaacgggcc aggatgttga ccatccaatt
     2041 caacaccaac aaattggtgt cggcgctggg aaatgctcct cctgaagtct tgagctttcc
     2101 accatgtcca aaaaattcgc cttccaatcc caggacgccg cccatggctc ctggatagac
     2161 acccgctttt agtccggctc cccctgaacc ctgaatattg gtcccccgaa acgtctcgtt
     2221 tgaaaatccg ttgcctccaa tcgacagatt cttatcgatc ggaaaggtgc ccagtaaata
     2281 gggggcaaaa taggcttcgg tcttcctagg ttctttggtg ggaatatccg cggaaggagg
     2341 ctcctgtgcg gcaacaactt gaggccccaa aaactcacta ctacacatta gccccatgac
     2401 gatcagtagc cagacctttt tcatgtaggc tccttctaga attcacccag gacgtgacca
     2461 gcctgggcaa ccctagcaaa cctcatgtga ccgtcaagtc attccatgga agcatagcag
     2521 ttgatgcggg agttgaatct cagcagattt gaccaacaca tggtgtagac gaaaggctgg
     2581 a
//
LOCUS       DAORQN010000206         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1209_length_2565_cov_12.125498, whole genome shotgun sequence.
ACCESSION   DAORQN010000206 DAORQN010000000
VERSION     DAORQN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_2565_cov_12.125498"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK614_08700"
     CDS             complement(<1..597)
                     /locus_tag="PK614_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB82324.1"
                     /translation="MNPPWVSRYRVDPDRQIVSWQPLFCHVPDDGDSGRLRDTPLSFV
                     GTLNRQWNPERVALIEGLQRRYPIAVGSGAYREPFNRSLMVLNQSAANDVNFRTFQAM
                     ACGALLIGERIGNGFNDLFQDRTHCALYDRGNVEQVIEIVAHYCGRPAERDAVARQGY
                     EAVMASHTSLHRAQAILQVVETAPLQGQIRVRRSRQLQI"
     gene            complement(628..1791)
                     /locus_tag="PK614_08705"
     CDS             complement(628..1791)
                     /locus_tag="PK614_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HRB82325.1"
                     /translation="MFGCSARPLSGRYLSFAEREEIALLRVQGYSMQEVARRLGRAAS
                     TISRELRRNAATRSGGLAYRATTAQWHAERAARRPKPAKLAQNAILRAYVEDRLAGVV
                     VAPNGAPVPGPAVPWKGRRHGPRQDRRWAQAWSPEQIARRLSVDFPEDETMRISHEAI
                     YQALFVHARGALRRELAAYLRTGRVLRMPRARTHRRGKAFISPEIMISQRPAEAADRA
                     MPGHWEGDLILGLGSSAIGTLVERTTRFTLLLHLPRMTGHGHEARVKNGPALAGHGAE
                     AVRDAITRTIITLPEQLRRSLTWDQGAEMAQHARLKIDAGVQVYFCDPHSPWQRGTNE
                     NTNGLLRQYFPKGTDLSVHSAEEVAAVAAALNARPRKTLDWKTPAEALDQLSR"
     gene            complement(1871..>2565)
                     /locus_tag="PK614_08710"
     CDS             complement(1871..>2565)
                     /locus_tag="PK614_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HRB82326.1"
                     /translation="GTWAGCLAVSVAAALPDARVLAIEASADALAVAQVNMEQHGLDA
                     RIECMQGDLLAPLAECGFVSQIDVIVSNPPYIPDLDLVTLQPEVRCFEPHLALAGGPD
                     GMDVHRRLLQQAPVYLKSGGVLLMEVGLGQAAAVCREAEESGWFRTYDVLRDEGGIDR
                     VVCFEKKEEGRNGGFKWSSQHLNEGGCDEHSKAAFGSVWAAPLAVTRSTVSGRRRGTA
                     TVLGSDRGRHGQ"
BASE COUNT          467 a          871 c          761 g          466 t
ORIGIN      
        1 aatctggagt tgccgcgaac ggcgaacccg aatctggcct tgaagcggcg ccgtctccac
       61 cacctgcaga atggcctgcg cccggtgcaa actggtgtgg gaggccatca ccgcctcata
      121 tccctgcctt gccaccgcat cccgctcggc cggacggccg cagtagtgtg cgacgatctc
      181 gatcacctgc tcgacattgc ccctgtcata gagggcacaa tgggtccgat cctggaacaa
      241 gtcgttgaaa ccgtttccga tccgctcgcc gatcaggagc gccccgcaag ccatggcctg
      301 gaaggtacgg aaattcacgt cattggcggc cgattgattc agcaccatca gggaacgatt
      361 gaagggttcg cggtacgcgc cgctccccac cgcaatggga taccgtcgtt gcaggccttc
      421 gatcaacgcg acacgctccg gattccactg ccggttgagt gttccgacga acgagagggg
      481 cgtatcccgt aagcggcccg aatccccatc gtcgggcaca tgacagaaca ggggctgcca
      541 cgagacgatt tgccggtcag gatcgacccg ataccgggag acccagggtg gattcaagtg
      601 gtcgtcgcaa cataggcttt gttggtccta tcgggacaac tggtcaagtg cctctgcagg
      661 cgttttccaa tcaagcgttt ttcggggcct ggcgttgagg gcggccgcca cggccgccac
      721 ctcctcggcg ctgtgcacgc tcagatccgt gcctttcggg aagtactggc gcagcaaccc
      781 attggtgttt tcgttggtgc cgcgttgcca ggggctgtga ggatcgcaga agtagacctg
      841 cacgcctgcg tcgatcttca ggcgtgcgtg ctgggccatc tcagcccctt gatcccaagt
      901 cagcgaccgg cgaagctgct caggcaaggt gatgatcgtg cgcgtgatgg cgtcacgcac
      961 ggcctcggca ccgtgtccgg cgagcgcggg tccgttcttc acgcgagcct catggccatg
     1021 gcccgtcatg cgaggcaggt gcagcagtag ggtgaagcgc gtcgtgcgct ccaccaaggt
     1081 gccgatcgcg gagctgccca gccccagaat gaggtcccct tcccagtggc ccggcatggc
     1141 gcgatcggcg gcctctgccg gacgttgact gatcatgatc tcgggcgaga tgaaggcctt
     1201 gcctcgcctg tgcgtgcgcg ccctcggcat ccgcaatacc cgcccagtgc gcaaataggc
     1261 cgccagctcg cggcgcagcg caccccgagc gtgaacgaac agcgcttgat agatggcttc
     1321 atggctgatg cgcatggtct catcttccgg gaagtcgacc gacaggcgcc gggcgatctg
     1381 ttccgggctc caggcttgtg cccaccgcct atcctgccgc ggtccatgcc gacggccttt
     1441 ccagggcacg gccgggccgg gcacaggcgc tccgtttgga gcgacgacga cgccagccag
     1501 tcggtcctcc acatacgccc gcaagatcgc gttttgcgcc agcttcgccg gcttgggacg
     1561 gcgagcggct cgctcggcgt gccattgcgc tgtggtcgcg cgataggcca gaccgccgct
     1621 tcgggtggcg gcgttgcgcc gcaactcccg ggagatcgtc gatgccgccc gcccgagtcg
     1681 gcgagcgacc tcctgcatag agtaaccttg cacgcgaagg agcgcgatct cctctcgctc
     1741 tgcaaacgag agataccggc cggagagcgg cctcgccgag catccaaaca tcgctggtgg
     1801 catgccgcct gccttccgga accatcgtgt tcctaccggc ggcgacacac cggcctcgat
     1861 tgcggcatcc tcactggcca tgcctgccgc gatcgctgcc cagaaccgtc gccgttcctc
     1921 tcctcctgcc actgacggtc gacctggtga cagcaagggg ggccgcccag accgatccga
     1981 acgccgcttt cgaatgttca tcgcaacctc cttcgttaag atgttgcgac gaccacttga
     2041 atccgccatt cctcccctct tcttttttct cgaaacagac cacgcggtca atgccacctt
     2101 cgtcccgcag aacatcgtag gtgcgaaacc atccgctttc ctcggcctcg cggcagaccg
     2161 ccgccgcctg accaaggccc acttccatca gcagcacacc acccgacttc aaatagacgg
     2221 gagcttgctg cagaagccga cgatgcacat ccattccatc gggcccgccg gctaacgcca
     2281 aatgcggctc gaaacatcgc acttccggtt ggagcgtcac cagatcgagg tcggggatat
     2341 agggaggatt cgacacaatg acgtcaattt gactcacgaa cccacactcg gccaacggcg
     2401 caaggagatc cccctgcata cattcaattc gtgcatcgag tccgtgttgt tccatattga
     2461 cctgcgccac agccaatgca tcagccgagg cctcgatcgc cagcactcgc gcgtcaggca
     2521 gggccgctgc cacagacaca gccaggcaac cggcccaggt tccca
//
LOCUS       DAORQN010000207         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1211_length_2564_cov_5.170984, whole genome shotgun sequence.
ACCESSION   DAORQN010000207 DAORQN010000000
VERSION     DAORQN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_2564_cov_5.170984"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..479)
                     /locus_tag="PK614_08715"
     CDS             complement(144..479)
                     /locus_tag="PK614_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB82327.1"
                     /translation="MALRKRPQARKPAKTTKVASPTKKRKPARVVKPAPVRAKRSEGL
                     TPRQKEILKLVSQGNTNRDIARRLDISVRTVEVHRFNLMRRLKVRNVAQLLRQALQQG
                     FLPKNFAFR"
     gene            578..1534
                     /locus_tag="PK614_08720"
     CDS             578..1534
                     /locus_tag="PK614_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB82328.1"
                     /translation="MPLISSALFEVCARLGWTHLSTHPGRTLLTIIGVGLGVAATIAV
                     QTANVDVLRSFEDSVLAVAGPVTLEVSAGEAGMDERLIASVRGIAGVDSAKPVGELGV
                     RVAGRSQSFLILGLDLLDELNSMQGRLPATLETLWRPGEGDEMDGLLAPSGLLLGQGL
                     ASELGVGPKALLNLEAGGREVSVAVTGVMGHRAEGPSVWDRLAVMDIAAFQRTFGLSG
                     RLDRIDVVTQPSSSVEQVAEAITRILPPAVTVRRPIQRSQQVESMVGAFQLNLSVLSM
                     VGLLIGMFLIYNTVSFTVAPLDETSCTMPGIRPCISALIGTT"
     gene            complement(1435..>2072)
                     /locus_tag="PK614_08725"
     CDS             complement(1435..>2072)
                     /locus_tag="PK614_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82329.1"
                     /translation="LASYAQSAIGAERCRSLVLEINLSQAQMRQQETSDMLRLRSGVD
                     PFPVLRFPNVAEWLGRVAKGACLEAHELRDIALVLGLIDDVQRYLLRHQADAPAVGAM
                     VVQLGPVEEYRRLTIALNAAIDPDGSMRESASPELHRLMQRANDLKQQMRHRLEQILH
                     SRRYEEVLQEHYFAQREGRYVVPIKAEMQGRIPGIVHDVSSSGATVKDTVL"
     assembly_gap    2073..2082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2358..>2564
                     /locus_tag="PK614_08730"
     CDS             2358..>2564
                     /locus_tag="PK614_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRB82330.1"
                     /translation="MTTRIGINGFGRIGRNVLRASLGDPSLEFVAINDLTDAKTLAYL
                     LKYDSVHGTLDASVEAKDDQLIIDG"
BASE COUNT          495 a          717 c          767 g          575 t
ORIGIN      
        1 tgggggtgtt tgagcgattg gagcgcgagc tggcggctga gttggccatg cggggcaagc
       61 agtcgcttga ggaatgtcgg gggaaactga aagagctgta aaaaaggtgc gccggttccg
      121 tacaggcacc ggcgcatgga gtgttagcgg aaggcgaagt tcttcggaag gaatccttgt
      181 tgtaacgctt gacgaagcag ctgcgcgacg ttgcggactt tcaagcgccg catcaaattg
      241 aagcgatgca cctcaacggt gcgcacgctg atgtccaggc ggcgggcgat gtcgcggttg
      301 gtatttcctt gggacaccag tttcagaatt tctttctgcc tgggcgtcag tccctcggat
      361 cgtttcgcgc gcacaggcgc cggctttacg acccttgcag gttttctctt cttggtcggt
      421 gaagcgacct tggtggtctt cgctggcttt ctggcttgag gtcgtttgcg tagtgccata
      481 tagatattgt accccaaatt ctactgaacg taatcttagc atatctgggt aaatctgtaa
      541 aaacaattac tagagtgttg ttccggtagg tctgccgatg ccgctgattt cctctgccct
      601 attcgaggtc tgtgcccgtc ttggatggac acatttgtcc actcatcccg gtcgcacgct
      661 ccttacgatc atcggcgtcg ggttgggggt tgcggcgacg attgcggtcc agactgccaa
      721 cgtggacgtc ctccgatcgt tcgaggattc ggtcttagcc gtggccggtc cggtcacact
      781 ggaggtatcg gccggagaag cgggcatgga cgagcgcctg attgcttccg tgagaggtat
      841 cgccggtgtg gattcagcta aaccggtggg agagctcggg gttcgagtgg ctggccgttc
      901 tcaatcgttc ctcatccttg gactggatct gctggatgag ttgaattcca tgcaagggcg
      961 cctccctgcg acgttggaga ctctgtggcg tccaggtgag ggggatgaaa tggacggcct
     1021 cctggctcca agcggactcc tgctcgggca gggtctggcc tctgaattgg gagtcggccc
     1081 aaaggcgctg ctgaaccttg aggccggtgg acgagaagtg tccgttgcgg tcacgggggt
     1141 gatggggcat agggccgagg ggccgtcggt atgggaccgt ctggccgtga tggatattgc
     1201 ggcgtttcaa cggaccttcg gtttgagcgg ccggttggat cgaatcgacg tggtgacgca
     1261 gccctcctcg tcggtcgaac aggtggccga ggcaatcaca aggattctgc ctccggctgt
     1321 cacggtgcgc cgtccgatcc agcgcagcca acaagtcgaa tctatggtag gcgcatttca
     1381 attgaacctc tccgtgctga gtatggtggg gctgctgatc gggatgttcc tgatttataa
     1441 caccgtgtcc tttacagtgg ctccgctcga cgagacgtca tgcacaatgc cggggatccg
     1501 gccctgcatc tcggccttga tcgggaccac gtagcgccct tcccgttgcg caaaatagtg
     1561 ctcctgcagc acctcctcat accggcgtga gtgcagaatc tgctcgaggc gatgccgcat
     1621 ctgctgcttg agatcattgg cgcgctgcat caacctgtgc agttcggggc tggctgattc
     1681 cctcatcgat ccgtcgggat cgatggcggc atttaaggca atggtcagcc ggcgatactc
     1741 ctcgaccggt ccaagctgca ccaccatcgc acccacggcc ggggcatcgg cctgatgacg
     1801 caggagatac cgctgcacgt catcgatcaa tcccaagacc aacgcaatgt ccctcaactc
     1861 gtgcgcctca agacaggcac ccttggcgac ccggcctaac cactccgcca cattgggaaa
     1921 tcggaggact ggaaagggat cgaccccgga cctcaatcga agcatgtccg acgtttcctg
     1981 ctggcgcatt tgggcttgcg acaggttgat ttccagaacc agtgatcgac accgctctgc
     2041 cccgattgca gactgggcgt aggaggccag gtnnnnnnnn nngcttggcc cattccagca
     2101 cgttgcacga ttcggactgt aaggtttcag attgcatcgc accccgactg ccggttgatg
     2161 gttacgaaaa aacggactct acccgaccgc ccgggcacag gcaaggcaag cccggaccgt
     2221 gtgaatgacg accggccact agccaacaac cacactcaat gtcctctttt tgtatgcttg
     2281 acacatagaa agtcggtcag tactatcgcc ccagcctcaa tcaattccac ccgtttaatt
     2341 cttgaggatt tagccccatg accacacgca tcggcatcaa tggattcgga cgcatcggtc
     2401 gcaacgttct tcgtgcatcc ctcggagacc ccagcctgga atttgtcgcc atcaatgacc
     2461 tcaccgatgc gaaaacattg gcctacttgc tcaagtacga ctcggtccat ggcacgctcg
     2521 acgcgtccgt ggaggccaaa gacgaccagc tcattattga cggg
//
LOCUS       DAORQN010000208         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1214_length_2558_cov_4.756292, whole genome shotgun sequence.
ACCESSION   DAORQN010000208 DAORQN010000000
VERSION     DAORQN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_2558_cov_4.756292"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PK614_08735"
     CDS             complement(<1..921)
                     /locus_tag="PK614_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HRB82331.1"
                     /translation="MMPKNIIVCTDGTWNHPTATDVTSENFTSADTNVFKLFALLAGE
                     ADMRSSTSRVKTIQGQVAFYDDGVGADGIWAVRVAEGATGAGLELKLRDGYQFISEQY
                     ESGDRLCLFGFSRGAFTARSLGGMLIRCGVPAASRRNDMFASHAFDVYRRNDPTMTAQ
                     YRADYQCQDVMIEVIGVWDTVGALGIPLGFFSPLDHLLFRFHDLSLHSSVRFGYHALA
                     IDEQRESFAPTLWDAREGVEQVWFAGVHADIGGGYKETGLSDLTLAWMLMKLQPHGIL
                     FGDRAFAPNGSPAIMGDPLMTPMHDSYKPPF"
     gene            complement(965..>2558)
                     /locus_tag="PK614_08740"
     CDS             complement(965..>2558)
                     /locus_tag="PK614_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82332.1"
                     /translation="LDGTGVAKVIIPMAEYKGMVVTEWGISAALNVIWRCGLTVPGID
                     GAQDWEVNAGGFFGASAAFSVANLDGTGDALFIIQATNSGWAGVLKWQNSGLYCVWQG
                     TADSWGLSYFNQHYPARMGGSGESLLAFAPGASIGLLTWSPGNLTCLTQSQYCVPGWN
                     LNFLTGLPATPFTSFTGTQLQLYQQICESLTPPALGADGQTGDIRYQYANLTNAETFA
                     GWATQIKDMPPISGYSQEDWDAVAPTIADEAGWVGTMYGFSADMAGLALAISIQQDKD
                     LNHCFGNLTLDPVTSPAPVSYWAGAVIDAALWGLAATPLGPEVQVGLALAASLFGSFV
                     GQPSSGGSPTTVVQYQDFKTTVDTLFSEALNGTYLDLQTVITDPVKLHVAGRLAEDPW
                     YWGITDNEIVAANGTNPNRIMFYQMLMAAEFSLFVWTNAKENYPAHRWLDWSGGHELT
                     PIDAPSWAWWSLPNADGTYTVSLLAQGTITEGGQGVNLVAFPIQTLMTDLFTNLQVQP
                     ADMTFQRFGWDKIQNPPNGDAW"
BASE COUNT          578 a          779 c          711 g          490 t
ORIGIN      
        1 aaagggcggc ttataggaat cgtgcatcgg cgtcatcagc ggatctccca taatcgccgg
       61 tgagccgttc ggggcgaagg cgcgatcgcc gaagagtatg ccgtggggct gcagtttcat
      121 gagcatccag gcgagggtga ggtcggagag cccggtttcc ttgtacccgc caccgatgtc
      181 ggcatgcact ccggcaaacc agacctgctc aactccctcg cgggcatccc acaaggtcgg
      241 agcgaagctt tcacgttgtt catcgatcgc cagggcgtga tacccaaacc gtacgctcga
      301 atgtaacgat agatcgtgaa atcggaacag gagatgatcg agcggactaa agaacccgag
      361 aggaataccg agcgctccaa ccgtatccca cacgccgatg acctcaatca tcacatcctg
      421 acactgatag tccgcgcgat attgagcggt catcgtcgga tcgttgcggc ggtagacgtc
      481 gaaggcatga ctggcgaaca tatcgttccg gcgcgatgct gccggcacac cacagcggat
      541 cagcattccc ccgagactgc gggccgtgaa ggcgccccgg ctgaatccaa agagacagag
      601 acggtctccg gactcatact gttcgctgat aaactgatac ccgtcgcgca gcttgagctc
      661 gagacctgca ccagtcgcgc cctccgcgac ccgtacagcc cagatgccgt cggcgcccac
      721 gccatcatcg tagaacgcaa cctgcccctg aattgtttta acgcgagagg tggacgaacg
      781 catgtccgct tccccagcca acagtgcgaa caacttaaag acgttggtat ccgccgacgt
      841 aaaattctcc gacgtcacgt cggttgcggt gggatgattc cacgtgccat cggtgcagac
      901 gatgatgttc ttgggcatca tgctttcctg aacatgtttt aggcgccctc actacgtcga
      961 catgtcacca ggcgtcgccg ttggggggat tctgaatctt gtcccacccg aaccgctgaa
     1021 aggtcatgtc ggccggttga acctgtagat tcgtgaacaa atcggtcatc agcgtctgaa
     1081 tcgggaaagc gaccaggttc accccctgcc ctccctcggt gatggttccc tgagcgagta
     1141 gggaaaccgt ataggttccg tccgcattcg ggaggctcca ccaggcccaa ctcggtgcgt
     1201 cgataggggt caactcgtgt cctcctgacc aatccaacca cctgtgcgcg ggataattct
     1261 ccttcgcgtt cgtccacaca aacaacgaaa actcggcggc catgagcatc tgatagaaca
     1321 tgatacggtt aggattcgtg ccgttggccg ccacaatctc attgtcggta atcccccaat
     1381 accaggggtc ttccgcaagc cgcccggcaa catgcagctt cacgggatcg gtgatcacgg
     1441 tttgcaggtc gagataggtt ccattcaatg cctcgctgaa taacgtatcg acggtggtct
     1501 tgaaatcctg atattgaacg acggtggtcg gcgagccgcc tgaggagggc tggccaacga
     1561 acgacccaaa cagcgaggcc gccaatgcaa ggccgacttg aacctcgggg cccagcggag
     1621 tcgctgcgag cccccacagc gcggcgtcga tgaccgcgcc ggcccaatac gaaaccggcg
     1681 ccggcgatgt gacgggatcc agggtgagat tgccgaagca gtggttcaag tccttgtcct
     1741 gctgaatgct gatcgccaat gcaagaccgg ccatatccgc ggaaaatccg tacatggttc
     1801 cgacccaacc agcctcgtcc gcaatggtcg gcgccacggc atcccaatct tcttgagaat
     1861 accccgatat gggcggcatg tccttgatct gcgtggccca tcccgcaaag gtctcggcgt
     1921 tggtcaaatt ggcgtattgg tatcgaatgt cgcccgtctg tccatcagct cccaaagccg
     1981 gtggggtcag gctttcacaa atctgctggt agagttgaag ctgcgtcccg gtaaacgacg
     2041 tgaaaggagt ggcaggcaat cctgtcagaa aattcaagtt ccaaccgggg acacaatatt
     2101 gagattgagt gagacaggtg agattccccg gcgaccacgt caacaacccg atggatgcgc
     2161 cgggcgcaaa ggccaacagc gactcacctg atccgcccat gcgcgcgggg taatgctgat
     2221 tgaaatagga aagtccccag ctgtcggccg ttccctgcca gacacagtaa agaccgctgt
     2281 tttgccactt caacacaccg gcccagccgc tgttcgtcgc ctgaatgatg aagagcgcat
     2341 cgccggtgcc gtcaagattc gccacagaga atgcagcgga agcgccgaag aacccgccgg
     2401 cattcacctc ccaatcctgg gctccgtcga tccccggaac cgtcagaccg caccgccaga
     2461 tgacgttgag agcagcggag attccccact cagtcaccac catcccttta tattccgcca
     2521 tgggaatgat gaccttggca actcccgtac catccaga
//
LOCUS       DAORQN010000209         2556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1215_length_2556_cov_5.390644, whole genome shotgun sequence.
ACCESSION   DAORQN010000209 DAORQN010000000
VERSION     DAORQN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_2556_cov_5.390644"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(519..2492)
                     /locus_tag="PK614_08745"
                     /pseudo
     CDS             complement(519..2492)
                     /locus_tag="PK614_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
BASE COUNT          521 a          780 c          740 g          515 t
ORIGIN      
        1 tattctccca cccaccagcc gtttccatcc gttcatagtg cttccgtgac cccatgcatt
       61 gttgtaccgc cttcacagag tcggtaaacg ccacgtcgct gatggatggt ttcatcgtcg
      121 tcctttcccg aacggatagg gccgctcccc cacactccgc ttacgcgtaa tggccgggaa
      181 ctgaccctgg tctcctactt atgcctggcg cggaaggctt ccgtcagacg ggttttgatg
      241 gcaggagagg gagacgaagc attgccgtgc cggcactgtt cgcccgaacg gcgcaatatt
      301 tgccgaacgg tgtgcaattg aactcggtat cgcgcacagg cgtcgcagat ccagtaatgg
      361 agccggagac tgagccgggt ggcccagggc agcggtcgat ccatagcttg agaagccaac
      421 cgggtgactt ctttgcaggg gagagtgata tgagccagta atgtggtgat catgttgcac
      481 aatgttcttc gcttcttccc gctccttacg actcgacatc agggattgag cgtcgcgtct
      541 ttcccccgcc atcgttgcac tgacttgccc ttcttaagct gagtcgatgg aggaaccgtt
      601 tccttacact ggcgcgcaag aatctgtttt gccgtgtttt tttcagcctt ggcctgaatg
      661 ccaaggtctt ccaccaaagc ccgcaggggc tccgcgatag accaggcttg ggcgaaacag
      721 ccctgcggcc gatgaggagc ttcagcgtcg aaaatttctg agacctgtcc cagacaggct
      781 tcatgcagat gcgcctcaag gccggcaaga aaaccgcgcg cggtcttccg cactgcggcg
      841 ctttcaccga atattttgac ccaggcggtc acaaacgggc ccaataagaa cggccagacc
      901 gtaccctgat gatacgcgcc gtcacgttct accacgcccc cttcgtatct cgggcggtag
      961 cgaggatcac gcggcgacag ggtgcggagc cccaccggcg tcaggagttg ctcttcgacg
     1021 aagcaaagaa tctgctgcgc gcggtcgtgc gggacgagtt cctcaaccaa cgagatggca
     1081 tagagttgat tgggacgaag cgacgcatcg tcgccttccg ggccgtcgat gacatcatac
     1141 agataaccgc cttgctcgta ccagaatcga ttgcggaacg aaatgatcgc gtggctgcgg
     1201 tcagtccggc accgatcggc atagtccgtt tcaccgtacc ggcgcgcgag tgtttcgccg
     1261 acttccagcg ctcgaaccca cagcgcttgg atctccaccg gttttccatg gcgcggggtg
     1321 acgacccagt cgccgacctt cgcgtccatc caggttaatt gtgcgccggg cactccaccc
     1381 gtaatcaacc catcctcatc catccgaatc ccataccgcg tgccgtggcg ataaccgtcg
     1441 aggatctgtt tcaccgccgg ccaggctgtt tgacggacac ggtcctcatg ctgcgatgcc
     1501 tgcaggtaac gatcgatcgc atggatgaac cagagcgagg cgtcgatcgt attgtattcc
     1561 ggctgctcgc cgacatcggg aaaacgattc ggcaccattc cctccgagac atgcgccgca
     1621 aaggactcga tgacctgcca ggcgacgtca tggcggccgg tgacaagaca gagccctggc
     1681 aatgaaatac acgtgtctcg cccccagtcg gtgaaccagg gatagccggc gatcaccgtc
     1741 tgtcgattcc ttcgttcgga cagataacat tccgcagcac gccggagttc gccggccaaa
     1801 gcgtcatcag ccggggcggc ttgtcggact gtgtcgcgac gaaccttctc gccctgcacg
     1861 agagcgccga cgtcgagccg gtcgatgacc tcgctggtaa ggaccatggt ctgaggggat
     1921 ccggactcaa gctcgaacgt gaactctccg ggcgaccacc agtcttcctc gctattcagc
     1981 cctcgttccc gttcgacggg aaattggagg tgccgatacc attccggttc atggcgatac
     2041 ccccctgaat gaaccgcccg caccggaggc acatcggggt agagctgcca ggcgacgagg
     2101 ccgtccccga tcgaagcctc ggtcgagagg ctcccgttct cgtgatgcgt cccatgatag
     2161 tcgcgcgcgg tgagtttcgg tctcacacga agcacggccc gctgtttctt cctgccgagc
     2221 agcgtccatc gcacaatggc cagatcgcgg ccctgaatca gcaggatctc ccgctgaagc
     2281 ctcataccgt tgcaggcgaa cgtccagatc ggccaggggt cggtggagaa ctcgacacaa
     2341 tgttcatacc cggtcggata aacggcgccc ggatagagat ttgtggacag tggaacgact
     2401 tgaccgtcga tcgcgagcca atcttcaagg tgattgacca acacaaaccg atcactcggc
     2461 ggtttgcgag cggtcaataa gatcgcatga taccggcggg tatggggtca cggaagcact
     2521 atgaacggat ggaaacggct ggtgggtggg agaata
//
LOCUS       DAORQN010000210         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1219_length_2552_cov_4.521426, whole genome shotgun sequence.
ACCESSION   DAORQN010000210 DAORQN010000000
VERSION     DAORQN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_2552_cov_4.521426"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PK614_08750"
     CDS             <1..1419
                     /locus_tag="PK614_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB82333.1"
                     /translation="AQGGLMPLRVRLTLWYGTALALILLTFSVVLYTITARGLRDQVD
                     ESLQETASAAVRSLEKRGFLPLIDEDALLSQFPELTRIDKFFQIFSPTGTITIRSPNI
                     RQHDVPLSRHALEAAFTGSTVFESARYPNEPPLRLVSVPIIYRGSLLYIVQVGTTLEG
                     VEETLRRFLLVLVVMAPIALVVSLVGGWFLAGRALRPVDSITIAAQRIAAGDLTQRLT
                     SERSTDEIGRLTDTFNNMIARLETSFAQIRQFSSDASHELRTPLTVMKGESELVLRRP
                     RPVEDYIAVLESNLEEIDRMSRIVEELLFLSRADLGQVKTECEPVRLEALVEDIQRQA
                     CLLGQERGVDVIMGTIQPATVRGDELRLRELILNIVDNAVKYSYPQGTVDIDLLVEGP
                     TARLSVRDHGIGIPQDAHKQIFDRFFRTDDARSHTKKGTGLGLAICAWIAEAHHGHIE
                     VQSEVGKGSTFTIVLPLAPLTA"
     gene            1534..>2324
                     /locus_tag="PK614_08755"
     CDS             1534..>2324
                     /locus_tag="PK614_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB82334.1"
                     /translation="MKRPTQSVSAIAGIAALSAALIWSYLPLTTSHASNPSPAAEERP
                     ATMTGTLPAAGFADVAKTVTPAVVNITTSGAEEVSDSSRPRGRMEDFFGSPFGPRRFG
                     PPMEPRERRGGGQGSGVIVTPDGYVLTNNHVIAGAKTVTVTLPDKREFKGRIIGSDPK
                     SDIAVVKIDGTQLPTVMWGDSSRLQVGEYVLAVGNPFGLNSTVTLGIVSALGRGHMGI
                     TQYEDFIQTDAAINPGNSGGALVNTRGELIGINTAIFSQTGGYQGV"
     assembly_gap    2325..2334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2357..>2552
                     /locus_tag="PK614_08760"
     CDS             2357..>2552
                     /locus_tag="PK614_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRB82335.1"
                     /translation="MSKPIYESLVKTGKVVRGYLGVGIQDLNQELAKSFNVKGSNGAI
                     VTDVKEEGPAGKAGLKQGDVI"
BASE COUNT          533 a          822 c          715 g          472 t
ORIGIN      
        1 gctcaaggtg gactgatgcc gcttcgagtc cggctcacac tctggtacgg aacggccctg
       61 gccctgatcc tgctgacctt ttccgtggtg ctctatacca tcacagcgcg cggcctccga
      121 gatcaagtcg atgagtcgct gcaagaaacc gcatcggcag ccgtgcgttc actggagaag
      181 cgcggatttc tccccttgat cgacgaagac gcgttgttga gccaattccc cgaattgacc
      241 cgcatcgaca aattcttcca gatcttcagt cccaccggca ccatcaccat ccgctctccc
      301 aacatccggc aacatgacgt gccattgagc cgtcacgcgc tggaagccgc ctttacgggc
      361 agcaccgtct tcgagtcggc caggtatccc aacgaacctc ccctccgcct ggtctccgtt
      421 cccatcatct accgcggctc cctgctctat atcgtgcaag tcggcacgac cctggaaggt
      481 gtcgaggaaa ccctccgccg gtttttgctc gtcctcgtgg tcatggcgcc gatcgctctg
      541 gtcgtctcat tggtgggagg atggtttctg gccggacgcg ccctccggcc cgtcgattcc
      601 attaccatcg cagcgcaacg cattgccgca ggcgacctga cacagcgcct gacctccgaa
      661 cgctccacgg atgaaatcgg acgactcacc gacaccttca acaacatgat cgcgcgcctg
      721 gaaacctcct tcgcgcaaat ccggcaattc agcagcgatg cctcgcacga actccggacc
      781 ccgctgaccg tcatgaaggg cgaaagcgaa ctggtcctgc gtcgccctcg tcccgtcgaa
      841 gactacatcg ccgtcctgga aagcaatctg gaagaaatcg accggatgtc gcgcatcgtc
      901 gaggaactcc tcttcctctc gcgggcggat ctgggccagg tcaaaacaga atgtgagcca
      961 gtccgcctgg aagccttggt ggaagatatc cagcggcaag cctgcctgct cggtcaagag
     1021 cgtggggtag acgtgatcat gggcaccatt caaccggcaa ccgtccgtgg cgacgaactc
     1081 cgcctgcggg aactcattct gaacatcgtc gacaatgccg taaaatactc ctacccgcag
     1141 ggcacggtgg atatcgattt gctggtggaa ggccccacgg cgcgcctttc agtgcgggac
     1201 cacgggatcg gcatccccca ggacgcgcac aaacaaattt ttgaccggtt cttccgcacc
     1261 gacgacgcac gctcccacac gaaaaagggg accgggctcg gcctggccat ctgcgcctgg
     1321 attgccgagg cgcaccatgg acacatcgag gtccagagcg aagtcggcaa agggtcgacc
     1381 ttcaccatcg tgctgcccct cgctccgctg acagcttaac aacttttaat ccctcgctaa
     1441 ttggtttctc atccacggtt gctaccgtcc agagtgtctg ctgcgggatc tctcccgcgc
     1501 acacagatca ccgacttcag gaggaggagc cccatgaaac gacctacgca gtccgtcagt
     1561 gccattgccg gcatcgccgc cctgagcgct gccttgatat ggagttatct cccgctcacc
     1621 acctcacacg cctcgaatcc ctcaccggcc gcagaggagc gcccggccac gatgacgggt
     1681 accctgcccg cagccggatt cgcagatgtg gccaagacgg ttacacccgc cgtcgtgaac
     1741 atcaccacga gcggcgcaga agaagtgtct gattcctcac gcccgcgtgg ccgcatggag
     1801 gactttttcg gatcgccgtt cggtccacgg cgattcggac caccgatgga accccgggaa
     1861 cgtcgtggcg gagggcaggg atcgggcgtg atcgtcaccc cggacgggta tgtgctgacc
     1921 aacaaccacg tgatcgcagg agcaaaaacc gtcaccgtca cacttccgga caagcgggag
     1981 ttcaaggggc gcatcatcgg cagtgacccc aaaagcgata ttgccgtggt caagatcgac
     2041 ggcacccagc ttccgacagt catgtggggc gactccagcc gcctccaggt cggtgaatat
     2101 gtcctggccg tcgggaaccc atttggtttg aactccacag tcaccttggg cattgtcagc
     2161 gccctcggcc gcggacacat gggaattacg cagtatgaag attttatcca gacggatgcc
     2221 gcgatcaatc caggcaacag cggcggcgca ctggtgaaca ccaggggtga gttgatcggc
     2281 atcaacaccg cgatcttttc tcagactggc ggctaccagg gcgtnnnnnn nnnncggctt
     2341 cgccgtcccc acgggcatga gcaaaccgat ctatgagagc ctggtgaaaa ccggcaaagt
     2401 cgtacgcggg tatctgggtg tcgggataca ggatctgaat caggagttag ccaagtcgtt
     2461 caacgtcaaa ggaagcaatg gtgccatcgt caccgatgtg aaggaagagg ggcctgcagg
     2521 caaggccggc ctgaagcagg gagacgtgat cc
//
LOCUS       DAORQN010000211         2539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1226_length_2539_cov_3.866747, whole genome shotgun sequence.
ACCESSION   DAORQN010000211 DAORQN010000000
VERSION     DAORQN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_2539_cov_3.866747"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1442)
                     /locus_tag="PK614_08765"
                     /pseudo
     CDS             complement(1..1442)
                     /locus_tag="PK614_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
     gene            complement(1555..>2539)
                     /locus_tag="PK614_08770"
     CDS             complement(1555..>2539)
                     /locus_tag="PK614_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRB82336.1"
                     /translation="IKAGLPVLRVWDLLIDRTQRAPFREALKAVRQDIRGGASASEAL
                     GKHSTYFSDLYLATIRAGEQSGNLAEVLQRYIAYLKLMIGLRQKVTKALAYPAVLVVV
                     GVAVGGLLLSYVMPTFISVYGESSANLPTATRMLISVIQMGEAQLIPVAIVAVVAVVM
                     GRAWYQTSAGRFSVDRNLLKLPLLGMILVEHHTIQLTRTLATVLAGGTPLVEALEIAR
                     GAVSNRFVSRGLVSAVNEIREGSTLAAAIERPQILPKLAIEMLSVGEETGSLEPMLRD
                     VAEFYEGDLDVRLSQLTTWIEPVLLLVMGLLVGGIVIIMYLPIFQMAGTIQ"
BASE COUNT          545 a          822 c          693 g          479 t
ORIGIN      
        1 cgggacgttg gtctttctcc ggtcgaaact gcagggcggg attcctccgc gagcgcctgg
       61 tcaaggacaa cctgatgccg gcaatcggga cagatcaccc gaatcagccg ttgcgccaga
      121 acgcaggtca acgccgccag aaagttgtag gaatcgatcc ccatcgaggc gaaacgtccg
      181 atcacatcga acacgttatt ggcgtgcacc gtcgtcaaca ccaaatggcc ggtcaaggcc
      241 gattgaatgg cgatctgcgc cgtttccgca tcacgaattt caccgaccat gatcttgtcc
      301 ggatcgtgtc gcagaatcga ccgcaaccca cgggcgaacg tcaggccctt cttctcgttc
      361 accgggattt gcacgacccc cggcaactga tactacaccg gatcttcgat ggtgatcagt
      421 ttatcttcct gaatgttcat ttcgctgatc gcggcgtaga gcgtcgtggt ctttccgctt
      481 ccggtcggac cggtcacgag caccatgccg tagggtctcg taatggcgcg acggaaccgc
      541 ttgaggtcct ccgggttgaa acccagacga tccagtctca gcgtcgatac gcccgtggta
      601 atcgcctcgc ggtccagaat tctgatgacg acggattccc cgaacacgct cggcaaaatc
      661 gacacccgga aatcgaccgt cttccggtcg agccgcatgc gaaaactgcc gtcctgcggg
      721 acgcgccgtt cggcgatatc gaggtcggac atgaccttga ggcgggacac taacggtgga
      781 tgcagcttca cgtcgagcgg atccatcgcc gagaccagaa tgccgtctac gcgaaacttc
      841 accgtggtgg ctcgatccgt cgcctcaatg tgaatatcgc tggcccgtcg ctgcatggcg
      901 ctcaacatga tcgtgtcgag gagtttgacc accgggcttt ggtcttcgcc gaagtgatcg
      961 acggtcaaga cctcttcgcc ccgctcgtcc tccttcacca ggaccgaccg gtactccgcc
     1021 tctaactcac gcagcgcctg actggaccct tcgctccgtt ccaatgccga ttggatggcg
     1081 cttcgcgagc tcaccacata gtggagcgga cgattgagca gaatctccaa ttcatccagc
     1141 gccagcaggt tctgcgggtc ggaaatcgca atcgtcaatg cgccggcctc gtccttcacc
     1201 ggcacgaaag gatgtcgccg catcaacttg acggaaatcg tgtgataaaa ctcggagtcc
     1261 acgcggaacc cggtgagcgg gtcatagggt aatccatact gggctgccaa tgcctgcgcc
     1321 aattgcgcct cggaaatggt cccctcttcg acgagcgtct gcccgaaggc ggtggtgctg
     1381 cctcccaaac gggcaacgac ctggtccacg gtccgtctcg gcaaaatgcc ctcgcgcacc
     1441 atcacatcgg cgagcgacgg gcgagtgaga tggcgttgca tcagcatatt cagtccctcc
     1501 gacttcggca cgggtcacga cgcgtcgggc acagggtccc gccgtatcgt cggattattg
     1561 aatcgtcccg gccatctgga aaatcggcaa atacataatg atgacgatgc ccccgaccag
     1621 gagccccatc accagcaaca acaccggctc aatccaggtg gtcaactgac tgagccgcac
     1681 gtccagatcc ccttcataaa actcggccac gtcacgcagc atcggttcca gcgagccggt
     1741 ttcttcaccg accgacaaca tttcaatcgc gagcttgggc aggatctgcg gccgctcaat
     1801 cgcagccgcc agcgtgctgc cttcgcggat ttcgttcacc gccgacacca acccgcgcga
     1861 cacgaagcgg ttcgagaccg caccacgcgc aatttcaagc gcttccacca gcggcgtccc
     1921 gccggctaac accgtggcca gcgtgcgggt caattggatc gtgtggtgct ccacaagaat
     1981 catcccgagc agcggcaatt tcaacaggtt cctatccacg gagaatcgac cggcggaggt
     2041 ttgataccac gcccgtccca tcaccaccgc cacaaccgcc acgatcgcca ccggaatcaa
     2101 ctgcgcctca cccatctgga tcaccgagat gagcatgcgg gttgccgtcg gcaaattagc
     2161 ggatgattca ccatacaccg agatgaatgt cggcatcacg taactcagca gcaacccccc
     2221 caccgcaacg ccgacaacca cgagaacggc cggataggcc agtgccttgg tgactttctg
     2281 acgaaggccg atcatcagtt tcagatacgc aatgtagcgt tgcaacactt ccgccaggtt
     2341 cccggactgc tcaccggcac gaatggtggc gaggtacaga tccgagaaat aggtggaatg
     2401 cttgcccaag gcttcagagg ccgacgcccc gccgcgaata tcctgacgga cggcttttaa
     2461 cgcttctcga aacggggctc gttgggtgcg atcgatgagc agatcccaga cccgcaagac
     2521 cgggagcccc gctttgatc
//
LOCUS       DAORQN010000212         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1232_length_2528_cov_5.467853, whole genome shotgun sequence.
ACCESSION   DAORQN010000212 DAORQN010000000
VERSION     DAORQN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1232_length_2528_cov_5.467853"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..365
                     /locus_tag="PK614_08775"
     CDS             153..365
                     /locus_tag="PK614_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82337.1"
                     /translation="MAENSAVYAIRFPDGSVTLYIDEEYAIDRGVDPAKLVRVEIPRE
                     LFASGSIQEIREYVAVYLENSHQGTA"
     gene            399..>843
                     /locus_tag="PK614_08780"
     CDS             399..>843
                     /locus_tag="PK614_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82338.1"
                     /translation="MTALLTPELIASTIEQAPIQGRIMLKLLLLQHFDITEEEITHIA
                     ADRPDPRCVAGSKPIHQVVKQDALRDVTSRRDEYRRRVRLRRERLWLQMEAMKRMIAL
                     AEGLIRAGSDLLLTQYKVDAAAVTALKQEARAAVPKPAIRLLNRRW"
     assembly_gap    844..877
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <878..1576
                     /locus_tag="PK614_08785"
     CDS             <878..1576
                     /locus_tag="PK614_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82339.1"
                     /translation="YQEARLGLEIQTQLRLLDKYRKRFELSEREWKSINAAPLQDHEI
                     GHIWGIPAGSLAARKVKHLHQYLQAVQNSLQRSSAKDLPATAPLDLWKETLAVLGERP
                     VERSIAQYDGLERTEDALVEKLRTFAWGTLGEDLEGKFWLSLVHGASSNAVHSENTRS
                     LFGLQRLMAVLSEIDTSPDALEQEMVTRTTPISKESLALPDEAAQPVVEEVGQMKQHV
                     LTSFMGELHDDLRR"
     gene            1685..2140
                     /locus_tag="PK614_08790"
     CDS             1685..2140
                     /locus_tag="PK614_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82340.1"
                     /translation="MYNPTIMLQRRDRRSFRPTAIGTLCLLGTLTMMAWALPVGVPYA
                     AALVEVTELLAHPDHYDHQVVTVSGRVSSFQLATNRDGHPAFGFLLQDTAGTVKVVGL
                     GKADVREGDYVVVEGIFSRLRQAGRAIVYNEIKATSVQSMARMNPDLVG"
     gene            complement(2137..>2528)
                     /locus_tag="PK614_08795"
     CDS             complement(2137..>2528)
                     /locus_tag="PK614_08795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB82341.1"
                     /translation="APLSYTHGQNEILQGLEAGLDGMERGAAKVITVEPVDAYGEIHP
                     EGFFEVQRERVPAEAQRIGIKLETTAPDGRVVFPYVAEIRPDVIVLDLNHPLAGRTLR
                     FDVRVVDILRGERQLVTEGARGSEKET"
BASE COUNT          519 a          755 c          705 g          515 t
ORIGIN      
        1 cctcgtcttt acgagttacg tgatcgcgcg gagacgggtc ttccgactct gagcactccc
       61 attcgagccc ccgacgcgtt ttgacactcg ggctggggct gtgttacctg taatagccat
      121 accgttccac aacatcagac ggagcagcct ccatggccga aaacagcgcg gtctatgcca
      181 tccgctttcc tgacggttcc gtgactctgt atatcgacga agaatatgcc atcgaccggg
      241 gcgtcgaccc tgccaagctg gtgcgggttg agattcctcg ggaattgttt gcgagcggca
      301 gcatccagga aatccgtgaa tatgtcgcgg tctaccttga aaacagccat cagggcacgg
      361 cctgagcgaa ccttttacgt cgcacgaggg gccacactat gaccgctttg ctcaccccag
      421 aactgatcgc ctcgaccatc gagcaggccc cgattcaggg ccgcatcatg ctcaagctgc
      481 tgctgttgca gcatttcgat atcacggagg aggaaatcac ccacatcgcg gctgatcggc
      541 ccgatccacg ttgcgtcgcg ggttcgaaac cgatccacca agttgtgaaa caggacgcgc
      601 tgcgcgatgt gacgagccgc cgcgacgaat atcgccgccg ggtccggttg cgacgagagc
      661 ggctctggct ccagatggaa gccatgaagc gcatgatcgc gctcgccgag gggttgattc
      721 gcgccgggtc ggacctgttg ctcacacagt ataaggtgga cgctgcagct gtgactgccc
      781 tcaaacaaga ggcccgtgct gcagttccca agccggcgat ccggctcttg aaccgtcgat
      841 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac caggaagccc ggctcggcct
      901 tgagatccag acacagctgc gcttgctgga caagtaccgg aaacggttcg aactctcgga
      961 acgggaatgg aaatcgatta atgccgcgcc cttgcaggat cacgaaatcg ggcatatctg
     1021 gggcattccg gccggaagtc tcgccgcgag gaaggtcaaa catctccacc agtaccttca
     1081 ggccgttcaa aattctctcc agcggtcgtc ggcaaaggac cttccggcga ctgcgccgct
     1141 tgacctctgg aaagaaaccc tcgccgtgct gggggaacga ccggtggagc gatccattgc
     1201 gcagtatgac gggttagagc ggaccgaaga tgcgctcgtg gagaagcttc gaaccttcgc
     1261 gtggggcacc ctgggggagg atctcgaagg gaaattctgg ctgtcactcg ttcatggcgc
     1321 cagttcgaat gccgtccatt ctgaaaatac ccgctcgctc ttcggcctgc aacgcctgat
     1381 ggcagtactg tccgaaatcg ataccagccc ggatgcgctc gagcaggaaa tggtgacccg
     1441 tacgaccccg atcagcaagg aatccttggc actgcccgat gaagccgccc aaccggtcgt
     1501 ggaagaagtc gggcagatga aacaacatgt gctgacaagc tttatgggtg agttgcacga
     1561 cgatctgcgg cgatagtcgc gtgctctggc agcgcgccgg cgcctgctta cgacgctggt
     1621 tgcacatcgt cccagttgtt cagaaaagtt ttttcacgtc ctccttctca gcctggagtg
     1681 aattttgtat aatccaacta tcatgctgca acgtagagac agacggtctt tccggcctac
     1741 cgccattggc accctgtgtc tgctcggcac gttgacgatg atggcttggg cattgccggt
     1801 cggtgtccca tatgccgcag ccttagtcga agtcacagaa ctcctggccc acccggatca
     1861 ctatgatcat caagtcgtca ccgtcagcgg gcgcgtaagc agttttcagc tcgccaccaa
     1921 tcgcgacggt cacccggcat tcggatttct cctgcaggac accgcaggaa cggtaaaagt
     1981 tgtgggcctt ggaaaagcag atgtgcggga aggtgattac gtggtggtgg aagggatatt
     2041 cagtcgtctt cgtcaggccg gccgcgccat tgtgtataac gaaatcaaag ccacatcggt
     2101 ccagtctatg gcccgcatga acccggacct ggtcggttaa gtctctttct cgcttcctcg
     2161 cgctccctcc gtgacaagct ggcgctcacc acgcagaata tccaccactc tcacatcgaa
     2221 ccgcaacgtc cggccggcaa gcgggtggtt gaggtcaagc acaataacgt ccggcctgat
     2281 ctcggcgacg taagggaata ccactcggcc atccggagcc gttgtttcca actttattcc
     2341 gatccgctgc gcctctgccg ggacacgttc tcgctgcacc tcaaagaatc cctcggggtg
     2401 aatctctcca taggcatcga ccggctcaac ggtgatgacc ttggccgctc cacgctccat
     2461 tccatcaagc ccggcttcca gtccctgcaa gatctcattc tgcccatggg tgtagctcag
     2521 cggcgcct
//
LOCUS       DAORQN010000213         2515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1236_length_2515_cov_274.761789, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000213 DAORQN010000000
VERSION     DAORQN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_2515_cov_274.761789"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="rapZ"
                     /locus_tag="PK614_08800"
     CDS             <1..774
                     /gene="rapZ"
                     /locus_tag="PK614_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRB82342.1"
                     /translation="VDNLPPALLPTFVELCHQQGGEIKNVALGIDIRERVFFGDLAKV
                     LGELKAQGHALQLVFLEAREEVLVRRFSESRRPHPLLPHMPVVEGVRFERERLSELRK
                     HADRVIDTSNITVHELRELLIRQFRQETAARRMTISLVTFGYKFGVPYDIDLLFDVRF
                     IRNPFFVPELKALTGEDARVQQYVLGDPAAGQFLEHLQGLFGFLVPLYERDQRSYLSI
                     GIGCTGGRHRSVTMALHLREQFAALGYDVSVTHRDMQKP"
     gene            910..1125
                     /locus_tag="PK614_08805"
     CDS             910..1125
                     /locus_tag="PK614_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRB82343.1"
                     /translation="MKKVSDVPKKKLYKTNEVCEMFDISRATLFRWEREGLITGPPRD
                     WRNWRLYTAENVEQIRHVMGGGRKEVA"
     gene            1133..2236
                     /gene="pilM"
                     /locus_tag="PK614_08810"
     CDS             1133..2236
                     /gene="pilM"
                     /locus_tag="PK614_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRB82344.1"
                     /translation="MSMLASLKNLADMEFLSMFSPQRQLLGLDIGSSSIKLVQIKEHR
                     GRYTLQKFAVKELEPEVIVDGTVMDEGRVVSAIKELLAEQNVKLKQVAISISGHAVIV
                     KKISLPPMPDEELDAQVKLSAEQYIPFDINEVNLDFHVLPPSENPDEQSEMSIVLVAA
                     KKDKINELTELVKAAGLTPVVMDVDAFAVENMYGVTSPATQDDTTILVNIGASVMNVN
                     IVGGGVSLFTRDIPLGGNRYSEAVQREMGVSFEEAEQLKKSDRDDDHTLASVMESVNA
                     EVASEIARTIDYFKTTSSESEIARVLLFGGGAKVKGLAQQLRDRMHVEVEIANPFNEI
                     DTSQCDVDPDQLTEMGPLAAVGVGLALRTVGDR"
     gene            2233..>2515
                     /locus_tag="PK614_08815"
     CDS             2233..>2515
                     /locus_tag="PK614_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82345.1"
                     /translation="MIRINLLATGPRSRKAKPQWDVRAEALIGIGVLLVTLTGCWYYA
                     ASLDEDIQAKQSEKQVKDKQVAQLKEQVKAVQDFEEKKKQLEAKNRIIDQ"
BASE COUNT          563 a          642 c          773 g          537 t
ORIGIN      
        1 gtcgacaatc ttccgccggc ccttcttccc acctttgtcg agttatgcca tcagcagggc
       61 ggggagatta agaatgtcgc cctgggcatc gatattcgcg agcgagtctt tttcggggat
      121 ttggcgaagg tcctgggcga gctgaaagcg cagggacatg cgctgcaact cgtgtttctg
      181 gaagcccgag aggaagtgct ggttcgccgg ttttccgaga gtcgccgtcc gcatcccttg
      241 ttgccgcaca tgccggtcgt ggaaggcgtg cggttcgagc gtgagcgcct cagcgaattg
      301 cggaagcatg ccgaccgggt tatcgacacc tccaacatta cggtgcatga attgcgcgag
      361 ttgctgatcc ggcagttccg ccaggaaacg gccgctcgcc gcatgaccat ctcactggtc
      421 acattcgggt acaagttcgg cgtgccctat gacatcgacc tgctcttcga cgtgcgtttc
      481 attcgcaatc cgttcttcgt gcctgagctc aaagcattga ccggggagga tgctcgcgta
      541 cagcagtatg tattgggaga tcccgcggcc ggtcaatttc tcgaacatct gcaaggcttg
      601 ttcggctttc tggtcccgct ctatgagcgg gatcagcgca gctacctcag catcggcatc
      661 ggttgtaccg gagggcgtca tcggtctgtg accatggcgt tgcacctccg ggaacaattt
      721 gccgccctcg gttatgatgt gtccgttacc caccgcgaca tgcaaaaacc ctagttcctg
      781 agcgctgttt cgccgtttct tcccgagaag cccctggaga gtccactttc ttgacaggtg
      841 gactatatca actatactta attccgtggg ctcgaaacat gcgatgaata gcggcgtttg
      901 aggcggttcg tgaagaaagt cagcgatgtc cccaaaaaga aattgtacaa gaccaacgag
      961 gtctgcgaga tgttcgacat ctcgcgcgcg acgttatttc gctgggagcg tgaaggattg
     1021 atcaccggac caccccgcga ctggcgaaat tggcgtctgt ataccgccga aaatgtcgaa
     1081 caaatcaggc acgtgatggg tggtggacga aaagaggtcg cgtagagagg gcatgagcat
     1141 gctggcttca ctgaaaaatc tggcggatat ggaatttctc tcgatgtttt ctccgcagcg
     1201 gcagttgctg gggctcgaca tcggttcgag cagcatcaaa ctggtgcaga tcaaggaaca
     1261 tcggggccgg tatacgttgc agaagtttgc cgtcaaggaa ttggagcctg aagtgatcgt
     1321 ggacggcacg gtcatggacg aagggcgtgt cgtgtcggcc atcaaggaat tgttggccga
     1381 acagaatgtc aaattgaagc aggtcgccat ctccatctcc ggccatgcgg tcattgtgaa
     1441 gaaaatttcc ctgccgccga tgcccgatga ggaactcgat gcacaggtga aactgtctgc
     1501 cgaacagtac attcccttcg acatcaatga agtgaacctg gactttcatg tcttaccccc
     1561 gtcggagaat ccggacgagc agagcgaaat gtcgattgtg ctggtcgcgg cgaaaaagga
     1621 caagatcaac gaattgaccg aactggtgaa ggcggccgga ctgactccgg tcgtgatgga
     1681 tgtcgacgca tttgcggtgg agaacatgta tggcgtgacc tcgcccgcca cgcaagacga
     1741 caccacgatt ctggtgaaca tcggggccag cgtgatgaat gtgaatatcg tgggaggggg
     1801 agtctccctc tttactcgcg atattccctt gggagggaac cggtattccg aggccgtgca
     1861 gcgggagatg ggcgtctcgt tcgaagaggc ggagcaactc aagaaaagcg accgagacga
     1921 cgatcataca ctagcatcgg tcatggagag tgtgaatgcg gaagtggcgt cggagatcgc
     1981 ccgcacgatc gattacttca agacgacctc ttcggagagc gaaattgcgc gggtcttgct
     2041 gttcggcggg ggagccaagg tcaagggcct ggctcaacag cttcgcgatc gcatgcacgt
     2101 cgaggtggaa atcgcgaatc cctttaatga gatcgacact tcgcagtgcg acgtcgatcc
     2161 cgatcagttg accgaaatgg ggccgcttgc ggcggtgggg gtaggcctcg cgctccggac
     2221 ggtgggggac cgatgattag aatcaacttg ctcgcaactg gaccccggtc caggaaagcc
     2281 aaaccgcagt gggatgtacg tgctgaggcc ttgatcggca tcggggtgtt gctggtcacc
     2341 ttgacgggtt gttggtatta cgccgcttcc cttgatgagg acatccaggc gaagcagagc
     2401 gagaagcagg tcaaggacaa gcaggttgct cagctgaaag agcaagtcaa ggccgtgcag
     2461 gactttgaag agaaaaagaa gcagcttgaa gcgaagaatc gcatcatcga ccagc
//
LOCUS       DAORQN010000214         2505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1244_length_2505_cov_8.013061, whole genome shotgun sequence.
ACCESSION   DAORQN010000214 DAORQN010000000
VERSION     DAORQN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_2505_cov_8.013061"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PK614_08820"
     CDS             <1..883
                     /locus_tag="PK614_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82346.1"
                     /translation="LQIELYIMPDQQGTFMTTRPHSFAQQIQTVAELMLAVAGESVSV
                     IKRAAPEQALKLKHQDEWTIYLEFLRVMFNLTDRVSALHIPLKEQPQFMDELTDTVID
                     QLKKALEPAFGAGSDQTEIVLTIGTAVAESRQTYERYRFLVTEDSPAKNDMYRDFSDR
                     VACAVGAPRNPGVTAAATLCVSAVIPTLIGIFEGQAPPVAADATANPATAGGSSAPRG
                     ATGADIKLVSVMSSIKGEEVETRWGLHPRFRQDLTQEEAKQLTTTMNRVAKILGERYA
                     TVAFSDQWASWHKAGHA"
     gene            953..>2505
                     /gene="glgP"
                     /locus_tag="PK614_08825"
     CDS             953..>2505
                     /gene="glgP"
                     /locus_tag="PK614_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRB82347.1"
                     /translation="MQAPDHIQPPQDTVPQSLHRLGELARNLWWSWNQPARQLFESID
                     PTLWFLTHHNPVQLLSGVKPERFVTLAGDPNFVRQYSAVLRAFDEYLSNKNTWAATEF
                     SALQNSPIAYFSAEFGLHISIPIYSGGLGILAGDHCKEASDLGIPLVGIGFMYPQGYF
                     KQRINPEGWQEATYAPFNRHDSPIHQAMTPTGIPCSIKVEIGHRQVTVLVWQVRNGRM
                     SLYLIDTDVPENTPEDRALSARLYGGDQEIRLCQEFLLGIGGVRILRALGINPAVWHC
                     NEGHSAFLTVERIREFVTTGHSHAEASDLVRQSTVFTTHTPVPAGHDIFPFHLMDRYF
                     SNYWGQLGLSREEFLRLGETPENAGHGFNMTALAMRLSAHVNGVSREHGRVSRQMWQH
                     LWPGLAQDLVPIRSLTNGIHAPTWISPESNSLYAKYLSPDWAERVDDPTIWQRVTDLP
                     DDALWAVRQTTRRKLMRFIRERARDGWIRGHLQPMQAIARGTLLDPEALTIGFARRFA
                     TYKRATLLFR"
BASE COUNT          546 a          812 c          682 g          465 t
ORIGIN      
        1 ccttcaaatt gagttgtata taatgccgga ccagcagggg acttttatga cgaccagacc
       61 gcattcgttc gctcaacaga tccaaaccgt ggcggagctc atgctcgcgg tcgcgggaga
      121 gtccgtctcc gtgatcaaac gggcggctcc ggaacaggcc ttgaagctca aacaccagga
      181 tgagtggacc atttaccttg aattcctgcg cgtcatgttc aacctgaccg accgcgtgtc
      241 agcgctccac atcccgctca aggagcaacc gcaattcatg gacgagttga ccgacacggt
      301 catcgaccaa ctcaagaaag ccctggaacc ggccttcggt gccggcagcg accagacgga
      361 aatcgtactg accatcggaa cggccgtggc cgaaagccga cagacctatg agcgctaccg
      421 gtttctcgtg acggaagaca gtccggcaaa gaacgacatg taccgggatt tcagtgatcg
      481 cgtcgcctgt gcggtcggag ctccacgcaa tccgggtgtg accgccgcgg ctacgctctg
      541 tgtcagcgcc gtcattccga ccctcatcgg gattttcgaa gggcaagcgc cgcccgtcgc
      601 tgccgatgcg acagccaacc cggccacggc tgggggaagc agcgcgcctc ggggagccac
      661 cggcgccgat attaagctgg tgagtgtcat gtcgagcatc aaaggcgagg aggtcgagac
      721 gcgatggggg ctccatccgc gtttccgcca ggacctgacg caagaggagg cgaaacaact
      781 gacgacgacc atgaatcgag tcgccaaaat tctcggagag cgctacgcca ccgtcgcctt
      841 ttctgaccaa tgggcatcct ggcacaaagc cggacacgcc tgacgcagca ttcaggaaac
      901 gggacaacac agtccccttc ctcacccatc gatcacattc cacggaggcg tcgtgcaagc
      961 acccgaccac atacaacccc ctcaagatac ggttccccaa agcctgcatc gactcggtga
     1021 actggcccgc aatctctggt ggagttggaa ccaaccggca cgtcagctgt ttgagtccat
     1081 cgaccctaca ctctggttcc tcacccatca caacccggtc cagctgctct ccggcgtgaa
     1141 acctgaacgc tttgtaacct tggccggcga tccgaatttc gtccggcaat actccgcggt
     1201 gttacgcgcc ttcgacgaat atctgagtaa taagaacacc tgggcggcaa cggagttctc
     1261 cgcactccag aattctccca tcgcctactt ttcggccgag ttcggactcc acatttctat
     1321 tccaatctat agcggcggac tcggcattct tgccggagac cactgcaaag aagccagcga
     1381 cttgggcatt cctctcgtcg gcatcggctt catgtacccg caaggatatt tcaagcagcg
     1441 tatcaacccg gaaggatggc aggaagccac ctacgccccc ttcaatcgac acgattcacc
     1501 gattcaccag gcgatgacgc cgacggggat cccctgctcc atcaaggtcg agatcggcca
     1561 ccgccaggtt acggtgctgg tgtggcaagt acgtaacgga cgaatgtccc tctatctcat
     1621 cgataccgat gtgccggaaa acacgcccga agatcgcgcc ctctccgcgc ggctctacgg
     1681 tggagaccag gaaatcaggc tctgtcagga gtttctgctc gggatcggcg gcgtgcggat
     1741 cttgcgcgcc ctgggcatca atcccgcggt ctggcattgc aatgaaggcc attcggcctt
     1801 cctgaccgta gaacgcatcc gggaattcgt caccaccgga cacagtcatg ctgaagcgag
     1861 tgacctggtt cgacagagca ccgtcttcac cacccacacc cccgttccgg ccgggcacga
     1921 tatttttccg tttcacctga tggatcgcta cttcagcaat tattggggcc aactcggact
     1981 ctccagagaa gagttcctac gcctgggcga aacgccggaa aacgcaggac acggcttcaa
     2041 catgaccgcc cttgcgatgc ggctgtcggc ccacgtcaac ggcgtgagcc gcgagcacgg
     2101 gcgagtctct cgacaaatgt ggcaacactt gtggcctggc ctggcgcagg atctcgtccc
     2161 tatccgcagc ctcaccaacg gcatccacgc gcccacctgg atctccccgg agtcgaactc
     2221 cctctatgcc aaatatctca gccctgactg ggcggaacgg gtcgatgacc ccaccatttg
     2281 gcaacgggtc acggacctgc cggatgacgc tctttgggcc gttcgacaaa caacgcgacg
     2341 caaactgatg cggtttattc gcgagcgcgc gcgggacggc tggattcgcg gccatctcca
     2401 gcccatgcaa gcaatcgcca ggggaacgct gctggacccg gaagccctca cgatcggatt
     2461 tgccagacgg ttcgcgacct acaaacgtgc gacactcctg ttccg
//
LOCUS       DAORQN010000215         2473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1258_length_2473_cov_28.455335, whole genome shotgun sequence.
ACCESSION   DAORQN010000215 DAORQN010000000
VERSION     DAORQN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_2473_cov_28.455335"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="pnp"
                     /locus_tag="PK614_08830"
     CDS             <1..1119
                     /gene="pnp"
                     /locus_tag="PK614_08830"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRB82348.1"
                     /translation="AHGSAVFTRGETQSLAVVTLGTTDDEQRIDALEGEYMRTFMLHY
                     NFPPFSVGEARPLRSPGRREVGHGALAERALKSILPGKDKFPYTVRIVSEILESNGSS
                     SMATVCGGTLALLDAGVPIKEPVAGIAMGLIKEGNQVLVLSDILGLEDHLGDMDFKVT
                     GTKNGVTALQMDIKIGGITSELMREALAQAKAGRLHILGCMAQALTEPRTKLSAFAPR
                     IFPMKIKQDKIRDVIGPGGKMIRSIIAETGVKINVEDTGDVTIASSDEASAQKAIDMI
                     KRLTEEVEVGKIYLGTVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVTDEVSEGD
                     QVMVKVLEIDKQGKIRLSRKEAMPAPAGSPATEPTPAG"
     gene            1126..2379
                     /locus_tag="PK614_08835"
     CDS             1126..2379
                     /locus_tag="PK614_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB82349.1"
                     /translation="MYRKIVLDNRLRIVAELLPTLKSVTIGIWVNVGSRDEQPGEEGL
                     SHFLEHMFFKGTRSRTATQISREIDALGGEMNAFTTRETTTFYVKVLDQQLEAALELL
                     SDLFYRSRFESKEVEKEKQVVLEEIRMVQDDPEDLVQELHMKHTLGSHPLGRPILGQA
                     PRIQALGRNDLVSYVGSHYDPERTVVAVAGNFTWRRLEQLLARYFSDSHKGVAARPSR
                     RPPEVKGGVLVKRKALEQVHLCLGLQGLSAGHKDRYAAHALNGVLGGSVSSRLFQEVR
                     EKRGLVYSIYSFLSTYSDGGMTTVYAGTRPKEVERVVEVVCRELRKLRTHGIDAKDLA
                     RVKNQMKGSLMLSLESSHSRMSKLAKDELTQGNHVSLEQMIAEIDRVTTDQVYRVAQT
                     LLDQRCLSITALGPIPTKSLQSFAS"
BASE COUNT          528 a          629 c          805 g          511 t
ORIGIN      
        1 gcgcacggat ccgcagtctt cactcgcgga gaaacgcaga gcctggcggt ggtgacgctg
       61 ggtacgactg acgacgagca gcgcattgat gccttggagg gcgaatacat gcgcaccttc
      121 atgttgcatt acaatttccc gcccttcagt gtcggcgagg cacggccgct ccggtcgccg
      181 ggccgccgcg aggtcgggca tggagctcta gccgagcgag cgctcaagtc gattcttccc
      241 ggcaaggata agtttcctta cacggtgcga attgtatcgg aaatcctgga gtcgaacgga
      301 tcctcttcca tggccacggt ctgcggcggt acgttggcgc tcttggatgc gggcgttccg
      361 attaaggagc cggtggcggg gatcgcgatg gggttgatca aggaaggcaa tcaggtgctg
      421 gttctgtccg atattctcgg attggaggat cacctgggcg atatggactt taaggtcacg
      481 gggacgaaaa acggcgtgac ggcgcttcag atggatatca agatcggcgg catcacctcc
      541 gaattgatgc gcgaagcgct ggctcaggcg aaggccggaa ggctccacat tctcgggtgt
      601 atggcccagg cgttgaccga accgcgcacc aagctgtcag ccttcgctcc gcgcatattc
      661 ccgatgaaga tcaagcaaga caagatccgc gatgtcatcg gccctggtgg gaagatgatc
      721 cgcagcatca tcgcggagac cggtgtgaag atcaatgtcg aagacaccgg cgatgtgacg
      781 attgcctctt ccgatgaagc atcggcacag aaggccatcg atatgatcaa gcgcctgacc
      841 gaggaagtcg aggtcggcaa gatctatctc ggaaccgttc ggaagatcat ggatttcggc
      901 gcatttgtcg aagtgcttcc cggcacggac ggattggtac atatctccca gctggctcat
      961 catcgcgtca aggcggtcac cgacgaggtg tccgaaggcg atcaggttat ggtgaaggtg
     1021 ctggaaatcg ataagcaggg gaagattcgc ttaagccgga aggaagccat gccggcaccg
     1081 gctggttctc ctgccaccga accaacgccc gcgggataat cgatcgtgta tcgcaagatc
     1141 gttctcgaca accggttgcg cattgtggcc gaactgctcc cgacgttgaa gtccgtcacg
     1201 atcggcatat gggtcaacgt cggatcgcgc gatgagcagc cgggggaaga ggggctgtct
     1261 cacttcctgg agcatatgtt cttcaaggga acgcgcagcc gcacggcgac gcagatttcc
     1321 cgcgagatcg atgcgctggg gggtgagatg aatgcgttca ccacccgaga aacgacgacc
     1381 ttttatgtga aggtgctgga tcaacagttg gaggcagctt tggagctgct ctcggatctg
     1441 ttctatcggt cccggttcga gtcgaaagaa gttgaaaaag aaaagcaggt ggtgctcgaa
     1501 gagatccgga tggtccaaga cgatccagag gatctggttc aagagcttca catgaagcac
     1561 acgctaggaa gccatccgct gggcaggcca atcctggggc aagctccgag aattcaggct
     1621 cttgggcgta acgatctggt gtcctacgtt ggttcgcact atgatccgga gcgcaccgtc
     1681 gtcgcggtgg ccggcaactt tacctggcgc cgcctggagc agttgttggc gcggtatttt
     1741 tccgactcgc acaagggtgt ggccgctcgg ccaagccgtc gcccgcctga agtgaagggt
     1801 ggggtgctgg tcaaacgtaa ggctcttgaa caggtccatc tttgtttggg ccttcagggt
     1861 ctgagtgccg ggcataagga tcgatatgcg gcgcatgcgc tcaacggggt gctaggcggg
     1921 agtgtaagct cacgcctgtt tcaggaggtg cgggaaaagc gtggcctcgt gtactcgatc
     1981 tattcgtttc tgtccactta ttccgatggc ggaatgacca cggtgtatgc gggtacgcgc
     2041 ccgaaagagg ttgagcgtgt cgtggaagtt gtctgccggg agctgaggaa gctccgcacg
     2101 catggaattg atgcgaagga tttggcccgg gtgaaaaacc aaatgaaggg cagtctgatg
     2161 ctcagcctgg agagttcgca cagtcgcatg agcaagttgg cgaaggacga actgacgcaa
     2221 ggcaatcacg tgtcgctcga gcagatgatt gcggaaatcg atcgcgtgac gaccgatcag
     2281 gtatatcggg tggcgcaaac gttactggat cagcgctgtc tttcaattac ggcacttggc
     2341 ccgattccga ccaagagtct gcagtcgttt gcgtcataac cggtcaattg cggggcaccc
     2401 gtaggcgggg cgcaagggct ggtcgggcaa tgagtagcgg tgatttgagc gccattgcag
     2461 ggctaactct ttg
//
LOCUS       DAORQN010000216         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1260_length_2470_cov_9.993375, whole genome shotgun sequence.
ACCESSION   DAORQN010000216 DAORQN010000000
VERSION     DAORQN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1260_length_2470_cov_9.993375"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1235
                     /locus_tag="PK614_08840"
     CDS             210..1235
                     /locus_tag="PK614_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB82350.1"
                     /translation="MMRYTFGRGLAPALGGFWLALGLLIMPALAAAGASPASTPAPAK
                     RWAQFELVSTEPDGSVQVGKDVVLGITLGGVPAGTESVMAIIESPGFQSQTVSLSEDA
                     APAVFQGTAILEPNSILKSRTTVHPKAVRIRVSFARAKITGLEEFLKRDVYVTLGDRP
                     VEEAEAEVLPGPPAESPEADAEAVQKATEQVMAVNATIAEEDLLPLPPPGESKAYWKQ
                     VSDLISRNWSRQIRSIRRAPSSETVRVHFKMYASGVAQLIQLEKGSGARDVNDAGLQT
                     IIHAHPFPPIPPDISSDVVDVHVRMRTGAKVATRDVQMTVEKKPSKPVVPPSPKEGTA
                     ASGPAKE"
     gene            1238..1849
                     /locus_tag="PK614_08845"
     CDS             1238..1849
                     /locus_tag="PK614_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRB82351.1"
                     /translation="MTNRIGRAMAMWCMGATVLFGATLGHAETARYDVDLDHSIVEFK
                     VAHMVISKTTGHFKDYTGFIEMDPDAGTVKAIEATIKSASVTTNHEKRDAHLRNPDFF
                     DVEKYPMITYKMKSHKKTGDGYTAVGDLTLHGVTKEVTLTGNFNGVTKDPWGNTRAGF
                     TAEGKVNRKDFGMVWNKALDSGGLVVGDEVFIKLDIECIKAKS"
     gene            2217..>2470
                     /locus_tag="PK614_08850"
     CDS             2217..>2470
                     /locus_tag="PK614_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82352.1"
                     /translation="MWVGRLVTLLAVLWSLAAAEAGALEFTADQITKINGRTQKSNIF
                     YRDNMWRIEHHTMGPVNISIVRKDKKVVWLLLSRMKHFKTV"
BASE COUNT          528 a          661 c          812 g          469 t
ORIGIN      
        1 gtataggtca gccccggttg agcggcggtc ttcacgagtg cgcgcatggg ctcattataa
       61 caagacggtg ggaaatgcct agccgaagcg agaacttgcc cggccaggtt agtttaagcg
      121 gtctcctgcc tgaatgtgag catgccgttc gtccgaatgg cgagggtaaa atacgggttg
      181 aactgggtag ggctcctggg taggatgcga tgatgcgata cacgttcggc aggggtctgg
      241 ccccggctct cggcggattt tggctcgcgc taggccttct gatcatgcct gccctggccg
      301 cagctggtgc ctctcctgcc tcgacgccgg cgcctgcgaa gcggtgggcg caattcgaac
      361 tggtcagcac ggagccggat ggatcggtcc aggtcggcaa ggacgtcgtg ctcggcatca
      421 cccttggcgg ggtgcccgca gggaccgagt ccgtcatggc gatcatcgag tctcccggct
      481 ttcagagtca gacggtgtcg ttgagcgaag acgccgctcc cgcagtcttt cagggtacgg
      541 cgattctgga gcccaattcc atattgaaga gccggaccac cgtgcatccg aaggccgtgc
      601 ggattcgggt gtcgttcgct cgcgcaaaaa tcacggggct cgaagagttt ctcaagcgcg
      661 atgtctatgt cacgctcggt gaccggccgg ttgaagaagc cgaagccgag gtgcttcccg
      721 ggccgcctgc cgagagccct gaagccgatg ccgaggcggt acaaaaggcg acggagcagg
      781 tgatggcggt gaacgcgacc attgccgagg aagatctctt gcccttgccg ccccccggag
      841 aatccaaagc ctattggaaa caagtgagcg atttgatcag ccggaactgg agccggcaga
      901 tacgctctat acgccgggct cccagcagcg agacggtgcg tgtgcatttc aagatgtatg
      961 caagcggtgt ggcgcagttg attcagttgg agaaaggctc gggagcgcgg gatgtcaacg
     1021 acgctggact gcaaacgatc atccacgccc atccgttccc tccgataccg ccggatatca
     1081 gcagcgatgt cgtcgatgtg catgtgcgca tgcgaacggg ggcgaaagtc gcgacccgcg
     1141 acgtgcagat gacggtcgag aaaaaaccgt ccaagcctgt cgtgccccca tcaccgaagg
     1201 aaggcaccgc cgcaagcggt cctgcaaagg agtagctatg acgaatcgga tcggtcgtgc
     1261 gatggccatg tggtgtatgg gcgcaacggt actgttcggc gcgacgctcg ggcatgccga
     1321 gacggcgcga tacgatgtgg atctggatca ttcgatcgtg gagttcaagg tcgcccatat
     1381 ggtgatttca aaaaccaccg ggcatttcaa agactacacg ggatttatcg aaatggaccc
     1441 cgatgccggg acggtcaagg cgatcgaagc gacgatcaag tcggcctcgg tgacgaccaa
     1501 ccatgaaaag cgcgacgcgc atctgcgcaa tcccgatttc ttcgatgtcg agaagtaccc
     1561 gatgatcacg tacaaaatga agagccacaa aaagacgggc gacggctata ctgccgtcgg
     1621 tgatctgact ctgcatggcg tgaccaaaga ggtcacgctc acaggcaatt tcaacggggt
     1681 gaccaaggat ccgtggggga atacccgggc cggatttacg gccgaaggca aagtgaaccg
     1741 gaaagacttc ggcatggtgt ggaacaaagc cctcgatagc ggagggctgg tagttggaga
     1801 cgaggtcttt atcaagctgg acatcgagtg tattaaggcc aagtcgtaag tcgaacaccc
     1861 cctctctcag aggccgcacg cctgcccggt caccatgccg ggcaggccga tccagccgcg
     1921 gcgcatgcag cgaggtctct ctgaatcagc gctgcgggtc gcccttccgt gataccggtt
     1981 gttaccgaca cagcagctgg ttgaggcgca gggaggatgt ccacgggcat ctggttctct
     2041 tgtcgggctg gtcctgtgcc tgcctcagtg gcggctcggg gctgtatgcc gatgggtggt
     2101 gtgggtgatg gatcagcaag ggtactaagg gtaattcgga ggccgagagt aggagcatcg
     2161 tcatgtgtcg caagcgggct ggaaaaattg gacctggcat cggtaggata cgcgggatgt
     2221 gggtgggcag gctggtgaca ttgctggcgg tgctctggag cctagccgcc gcggaggccg
     2281 gggcgctgga gtttacggcc gatcagatca ccaagatcaa tggtcgaacc cagaagtcca
     2341 acatcttcta tcgtgacaac atgtggcgga tcgaacatca cacgatgggg cccgtcaaca
     2401 tcagcatcgt gcgcaaagat aagaaggtgg tctggctcct gttgtcgcgt atgaagcact
     2461 tcaagaccgt
//
LOCUS       DAORQN010000217         2451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1271_length_2451_cov_5.142738, whole genome shotgun sequence.
ACCESSION   DAORQN010000217 DAORQN010000000
VERSION     DAORQN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_2451_cov_5.142738"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>872
                     /locus_tag="PK614_08855"
     CDS             <1..>872
                     /locus_tag="PK614_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB82353.1"
                     /translation="RLRDVLPLSPLFQYFDSAVVYEGETAFVQLVSAVGAKRSLADVP
                     NTIYKDATGVHVSATSFAEDMAALPPPDFDGLPLDKYFVPTKILPYLATRGCYWGRCE
                     FCDHGEGYTAGYRTKKIQDILAEVTYLRDKYGARHFHFTDESYPPALFRKLTRGLIDS
                     KMGIAWTTHMRFEKSLLEDQVWQDAKASGCKYLHFGYESGNERVLKLMDKATTTEIMT
                     KHLKYTAEVGIWNHCMGFFGFPGETREEAWSSVEFLEQNRDYVHSLGFGTFDLGRHNP
                     VAKHPEKFGVTAYK"
     assembly_gap    873..890
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <891..1132
                     /locus_tag="PK614_08860"
     CDS             <891..1132
                     /locus_tag="PK614_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRB82354.1"
                     /translation="EWDLALDYYFTVKQGLSIEEAERVFEEFERNHNPGWDLRLFIRE
                     YIFLYIARFGLQKLPDLQFRSARVATVPPSLAGKM"
     gene            1283..>2451
                     /locus_tag="PK614_08865"
     CDS             1283..>2451
                     /locus_tag="PK614_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRB82355.1"
                     /translation="MSSSGLVQIDGLSPIKKEERKTSKVMLLFPPEWVPTAPYLALPS
                     LTAVLREAGHTVVQRDINIEMYDHFFTTEFLIWVKGRLGMQLKPLQDKEKAGRLTEQE
                     AGQKAVLEEAYAVDVFDLADRAEDAKLVVRGERFYEAEKLELALNTFREAMQYISAAY
                     YPASLVFYPMESNLGYRPGVSKEVFACLDDEQVNVYRDICNQLVLPSVSKEKPTVIGI
                     SIGTQMQLMAGLTFCKMIKESFPDIHVVVGGNVVTRLQEEWAKHERFFTEVFDAAILY
                     EGEHALLWYIEAMNGQRAIESVPNLMYYESGAVQQSKEVYTEKTAALPLPDFDGLPLD
                     HYFVPERIIPYLATRGCYWGRCTFCDHGQGYFDQYRGMTAQHVVEQVTALRDKYQ"
BASE COUNT          505 a          649 c          787 g          492 t
ORIGIN      
        1 cgcgtctgcg cgatgtgctc ccgctgtcgc cgctgtttca atacttcgac agtgccgtgg
       61 tctatgaggg ggagacggcg tttgtgcagc tggtgtcggc ggtgggggcg aagcgaagcc
      121 tggccgatgt gcccaacacc atctataagg acgcgaccgg tgtgcatgtt tccgcgacga
      181 gtttcgccga ggacatggcc gcattgccgc cgccggactt cgacggcctg ccgctcgaca
      241 agtatttcgt gccgaccaaa attctgccct atctggcgac gcgcggttgt tactggggtc
      301 gctgcgagtt ctgcgaccac ggcgaaggct acacggcggg ctaccgcacg aagaagatcc
      361 aggacatttt ggccgaggtg acctatctgc gcgataagta cggcgcgcgg cattttcact
      421 tcaccgatga gtcgtacccg ccggccctgt ttcgcaagct gacgcgcggg ctgatcgaca
      481 gcaagatggg catcgcctgg accacgcaca tgcggttcga gaagagcttg cttgaggatc
      541 aggtttggca ggatgcgaag gcctccggct gcaaatatct ccacttcggc tatgagtcgg
      601 ggaacgagcg cgtgctgaag ctgatggaca aggcgacgac gaccgaaatc atgacgaagc
      661 atctaaagta cacggcggag gtcggcatct ggaaccactg catgggcttc ttcggttttc
      721 ccggtgaaac gcgggaggag gcctggtcgt cggtggagtt cctggagcaa aacagggact
      781 acgtgcattc gttgggattc gggacgttcg acctcggccg gcacaatccg gtggcgaaac
      841 atccggagaa gttcggcgtg accgcctaca agnnnnnnnn nnnnnnnnnn ccgagtggga
      901 cctggcgctc gattattact tcaccgtcaa gcagggattg agcatcgagg aggccgagcg
      961 ggtgttcgaa gagttcgaac ggaatcacaa tcccggctgg gacctgcggc tcttcatccg
     1021 cgaatacatt ttcctctaca tcgcacgctt cggattgcag aagctgccgg acttgcagtt
     1081 ccggtcggcg agggtggcca cggtaccgcc gtccttagcc ggaaagatgt gagggctggg
     1141 gtcagccccg tcgttcgtat ctcgtgaagc gcatgaagga tagtccctcg tcattcgtat
     1201 ttgacgagat acgcttcacg aaagacgaga tacggtttgt gaatgatacg ccggaaggcg
     1261 agtagataaa tcgaaggaac acatgagcag tagcggctta gtccaaatcg acggattatc
     1321 tccgatcaag aaagaggagc ggaagacctc taaggtcatg ctcctgttcc cgcccgagtg
     1381 ggtgccgacg gcgccgtacc tcgcgttgcc ttcgttgacc gccgtgttgc gggaggccgg
     1441 gcatacggtc gtccagcgcg acatcaacat cgagatgtac gatcatttct tcaccacgga
     1501 gtttttgatt tgggtgaagg gtcgtctggg catgcagttg aagccgctcc aggacaagga
     1561 gaaggcgggg aggttgacgg agcaggaggc cggtcagaaa gcggtgctgg aagaggccta
     1621 tgcggtagac gtgttcgatc tcgcggaccg ggcggaagat gccaagctgg tcgtgcgtgg
     1681 cgaacgattt tacgaggccg aaaagctgga actggcgctg aataccttcc gcgaagcgat
     1741 gcagtacatt tccgccgcgt actatccggc gtcgctggtg ttctatccaa tggaaagcaa
     1801 cctgggctat cggcccgggg tctccaagga agtctttgcc tgcctcgacg acgaacaggt
     1861 gaacgtctac cgcgacatct gcaatcagct cgtgttgccc agcgtgagca aggagaagcc
     1921 gacggtgatc ggcatctcca tcggcacgca gatgcagctg atggccggcc tcacgttctg
     1981 caagatgatc aaggaaagtt ttcccgacat ccacgtcgtg gtcggcggca acgtcgtgac
     2041 gcggttgcag gaagagtggg cgaagcatga gcgtttcttc accgaggtct ttgacgccgc
     2101 cattttgtac gagggcgaac atgcgctgct ctggtacatc gaggcgatga acggacagcg
     2161 cgcaatcgag tcggtgccca acctcatgta ttacgagtcc ggcgcggtac agcagagcaa
     2221 ggaggtctat acggagaaga cggcggcgct gccgctaccg gatttcgacg gtttgccgct
     2281 ggatcactat ttcgtcccgg agcggatcat tccctacctc gcgacgcgcg gctgttattg
     2341 gggccgctgc accttctgcg atcatgggca gggctacttc gatcaatacc gtgggatgac
     2401 agcgcagcat gtggtcgagc aggtgacggc gctacgggat aagtatcaat g
//
LOCUS       DAORQN010000218         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1273_length_2445_cov_12.925941, whole genome shotgun sequence.
ACCESSION   DAORQN010000218 DAORQN010000000
VERSION     DAORQN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_2445_cov_12.925941"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..904
                     /locus_tag="PK614_08870"
     CDS             68..904
                     /locus_tag="PK614_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB82356.1"
                     /translation="MPLEDELSDILKKARNGQQRSIADVTRASGLSEVEVSELERGQS
                     PRSREQVQAVARALGLRAEPLAQVVDGWTPEALPSSMPHVETVFGSIGGYEVKGYVVH
                     DRGEAVVVDTAYNASGMLALLAQRKLRLRAICLTHGHSDHAEGIEAILKAYPVPVYLG
                     PDDFDLLSWRPSPQVLHVPQGGETLPVGGLTIRFMPTPGHTPGGVCYRLEQAGAPICF
                     VGDTLFAGSIGRSNPAGLYETHLESVRKQVLTLAPDTRLFPGHGPATTVREELLHNPF
                     YS"
     gene            1124..2083
                     /locus_tag="PK614_08875"
     CDS             1124..2083
                     /locus_tag="PK614_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB82357.1"
                     /translation="MNRDDSSEQPRSPDELIPLSSDESDEDEWEEERPQFFAWMLPAL
                     LFLLTVFTVLWAGAYQTNTNPLVGPWNFLVDDPGSLWRGVPFAATLLGILVTHELGHY
                     VLSRIHGVPTSLPLFVPGLPHFVGTFGAIIRMRAPLTDRRALFDIGVAGPIAGFVVAV
                     IALVVGLRLSTVVPIQTSYGMHLGEPLLLQFASWLVIGPLAPTADVILHPIGFAAWFG
                     LFITSLNLLPIGQLDGGHVAYALLGDRQRSMAVALVPILMVFGWLGWKGWFLWVGLAG
                     MMGLAHPPVQNPHRELGGIRVLVGWIALAIFVLTFSWEPFILR"
     gene            2087..>2445
                     /locus_tag="PK614_08880"
     CDS             2087..>2445
                     /locus_tag="PK614_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82358.1"
                     /translation="MQCPKCGWEQGDEQVECARCGVIFAKLANAPRPVPATRPRLPVQ
                     DSAWFRLAEDRLLTTEESVNPFHFTGRVLAFLVLVLWGWRFMTTPLETNYTGESFLHL
                     LNTDARVAHDNLYPAIP"
BASE COUNT          399 a          711 c          785 g          550 t
ORIGIN      
        1 ggtgaaggaa attatgctgg tcccggagag ttaaactcgt tcgtgggttc ttaggccgga
       61 gactcctatg cctcttgaag acgaactcag cgatattctg aagaaggctc gcaacggtca
      121 gcagcggtcg atagctgatg tgacgcgtgc gagtggcttg tccgaagtcg aagtgagtga
      181 gctggaacgg gggcaatcgc cacggagtcg cgaacaggtc caggctgttg cccgggcgct
      241 tggactgaga gcggaaccgc tggcgcaggt agtggacggt tggactcctg aggccttgcc
      301 ttcctcgatg ccacatgtgg agaccgtgtt tgggtcgatc ggcgggtatg aggtcaaggg
      361 ctatgtcgtt catgatcgcg gagaagccgt cgtcgtcgat acggcctaca atgcgagcgg
      421 gatgttggcg ctgttggccc agcggaagct tcggttgcgg gccatctgtc tgacgcacgg
      481 tcactccgat catgccgagg gcattgaggc aatcctcaag gcatacccgg tgccggtcta
      541 ccttggccct gacgatttcg atctcttgag ctggcggcca tcgccgcagg tgttgcatgt
      601 gccgcaggga ggcgagacgc tgccggtcgg cgggctgacc atccggttca tgcccacacc
      661 gggccataca ccgggcggcg tctgttaccg tcttgagcag gccggtgccc cgatctgttt
      721 tgtgggggat acgctgtttg ccggctccat cggccgctcc aatccagccg ggctctatga
      781 gacccatctg gaatcggtcc gcaagcaggt gttgacgctg gctcccgaca caaggctttt
      841 cccgggacat ggaccggcta ctacggttcg tgaggagtta ctccacaatc cgttctactc
      901 ctagcagggt gcggaacaac tcgattgttg cgcaatacgt tatgatcaac tcgtgcgccg
      961 tgtcggtatc agaatcaccc gcaggatgcg caaaacggcc gtccagcaag gccgcagcga
     1021 gcgaaggggc gaatcgtact cgtgcagtac ggtgagccgc gaagcgatgc gagaacgccg
     1081 ccggcggcct ttttccgcat cctgctagga ctggaggccg ggcatgaacc gtgacgactc
     1141 gtcggagcag ccgcggtctc cggacgaact gattccactt tcgtccgacg agagcgacga
     1201 ggacgaatgg gaggaggaac gtccgcagtt ctttgcatgg atgcttccgg cgctcctgtt
     1261 cctgcttacc gtctttaccg tcctctgggc cggggcctac caaaccaaca ccaatccact
     1321 cgtcggcccg tggaattttc tcgtcgatga tcccggctcg ttatggcggg gtgttccatt
     1381 tgccgccacc ctgctgggca tcctggtgac gcatgagctc ggtcactatg tgttgtcgcg
     1441 cattcatggc gtgccgacat cgctgccgct cttcgtgccg gggttgccgc atttcgtcgg
     1501 cacgttcggc gccatcatcc gtatgcgggc gccgctgacc gaccgccggg ccctgttcga
     1561 tatcggcgtg gcggggccaa tcgccgggtt tgtggtggcg gtgatcgcgc tggtagtggg
     1621 ccttcggctc tcgacggtgg tgccgattca aaccagttat ggcatgcatc tcggcgagcc
     1681 gctcctcctt cagtttgcgt cgtggttggt gatcggcccg ctcgctccca ccgcggatgt
     1741 gatccttcat ccgattgggt ttgccgcctg gttcgggctc ttcatcacct cgcttaacct
     1801 gcttccgatc ggtcagctcg acgggggaca tgtggcctat gcgttattag gagatcggca
     1861 acgcagcatg gcggtcgccc tcgtgccgat ccttatggtc ttcggctggt tgggctggaa
     1921 gggttggttc ttgtgggtgg ggcttgccgg catgatggga ctggcccatc caccggtgca
     1981 gaatccccat cgtgaactag ggggaatccg tgtgctggtg ggttggattg ccctggcgat
     2041 ctttgtcctg accttctcct gggagccgtt tattttgcgg tagccgatgc agtgtccaaa
     2101 gtgcggatgg gaacagggcg acgaacaggt tgagtgtgct cgatgcggag tgatttttgc
     2161 gaagctcgcc aacgcgccac ggcccgttcc cgccaccaga ccgcgcctac cggtccagga
     2221 ctcagcctgg ttcagactcg ccgaggaccg gctgctcacg acggaggagt ccgtcaatcc
     2281 gttccacttc accgggcggg ttctggcgtt tctcgtgctc gtcctgtggg gctggcgctt
     2341 catgaccacg ccgctcgaga cgaattacac gggcgaatcg tttttgcatt tgctcaatac
     2401 cgatgcccgt gtcgctcacg acaatctcta cccagcgatt ccctt
//
LOCUS       DAORQN010000219         2440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1280_length_2440_cov_4.161845, whole genome shotgun sequence.
ACCESSION   DAORQN010000219 DAORQN010000000
VERSION     DAORQN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1280_length_2440_cov_4.161845"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK614_08885"
     CDS             complement(<1..580)
                     /locus_tag="PK614_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRB82359.1"
                     /translation="MTGHERPFQGAILTPVRWCVIMGSLSVVALILAVVCLQLGTQYI
                     GLGKLISVLSSALLDQPTDNDVLKTTNVILLQVRLPRVFLGFLVGVCLASVGVALQAL
                     LRNPLADPYVLGVSSGAALGVAVAVLFGIGTTVLAFSLLPVCGFLGGLVALLVVYRMA
                     ATYDRLPIHSVLLAGVILNAIFSALIMFITSIM"
     gene            complement(667..1809)
                     /locus_tag="PK614_08890"
     CDS             complement(667..1809)
                     /locus_tag="PK614_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRB82360.1"
                     /translation="MSQKTCLRETLYSLVGWGIGEDDIAGAILRVPCWPWGFFYLRAV
                     RIVSSRRFAASCIGVVASVLLACLAFAFGETQEDHTVMKRRQQGILTGMPFMANITPR
                     TFIDDAGRKLYVAKAPTRVVSLAPSITEMLFALGLDEQIVGVTEFCDFPAAAKAKSKV
                     GYANPNLESLVALRPELIIAPREFHRANVLAKLEELKIPVLLLEATSVENIFSHIHTL
                     GRIFDRSTEAHAMTAAMRSRMAEISSRTEHLPRIRVLYVINSQPLITVGPGSYIHQMI
                     GLAGGTNIASGAATPYPRLTMETVLKEDPEVLIFPMGSVETVPRREQQEWRRWTTLTA
                     VQQNRLREVSANALNRPGPRVMEGLEELVRVIHPEAFPPESVPVQP"
     regulatory      complement(1773..1955)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2077..2334)
                     /locus_tag="PK614_08895"
     CDS             complement(2077..2334)
                     /locus_tag="PK614_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRB82361.1"
                     /translation="MDQVTLIGLLAGTLTTIAFIPQLQQTWRTRSAQDVSLGMLLTFV
                     TGVFLWLIYGLMLGALPIILANLVTLVLTLAILILKLRYRS"
BASE COUNT          556 a          730 c          721 g          433 t
ORIGIN      
        1 ccataatcga ggtgatgaac atgatcagcg ccgagaaaat cgcgttcagg atcacccccg
       61 ccaacagcac ggaatgaatc ggaagccggt cgtaggtggc cgccatccga tagaccacca
      121 gcagggccac cagtccgcct aaaaacccgc agaccggcag gagcgagaag gcgagaaccg
      181 tagtcccgat cccgaacagc accgcgacag ccacacccag cgcggctcca ctggacaccc
      241 cgagcacata gggatcggcc agaggatttc tgagcagggc ctgcagcgcc acaccgaccg
      301 acgccaggca gactcccacc agaaatccca gaaagactcg cggcagacgc acctgcagca
      361 ggatgacgtt ggtcgtcttg agcacgtcgt tgtcggtagg ctggtcgagc agcgcggacg
      421 acagcacgct gataagtttc ccgagtccga tgtactgtgt cccgagttgc agacagacga
      481 cagccaggat cagcgcgacg accgacaaac tgcccatgat gacgcaccaa cgcaccggag
      541 tcaggatggc cccctgaaac ggccgttcat gtccggtcat tggagcggaa ctgtgacggc
      601 tcgtggaagg caacgggggc gaggtcaatg cgggagcagg tgacgtggag tggccgaccg
      661 aaggcatcat ggctggaccg gtaccgattc gggaggaaag gcctcggggt gaatcaccct
      721 cacgagttct tccaaccctt ccattacacg aggcccggga cgattgagcg cattggccga
      781 aacctcgcgc aaccggttct gctgcacagc ggtcagggtc gtccagcgcc gccactcctg
      841 ctgttcgcgt cgaggcaccg tttcaaccga ccccattggg aagatcagca cttcgggatc
      901 ttctttcagg accgtttcca tggtcaaccg tggatagggt gttgcagctc cggatgcgat
      961 attggttccc ccggccaggc cgatcatctg gtggatgtaa ctgccgggac cgaccgtgat
     1021 caagggctgg ctgttgatca catacagcac gcggatgcgc ggcaggtgct cggttcgact
     1081 gctgatctcg gccattcgac tccgcatggc cgcggtcatg gcatgggctt ccgtcgaacg
     1141 atcgaaaatg cgtcccagcg tgtgaatgtg cgaaaaaatg ttctccaccg aagtggcttc
     1201 cagcaggagc accggaatct tcaattcctc cagcttggcc aacacattcg cgcggtggaa
     1261 ctcccgcggc gcaatgatca attccggccg gagggccacg agggattcaa gattggggtt
     1321 ggcgtacccg accttggatt tagccttggc tgctgccggg aaatcgcaaa actccgtgac
     1381 cccgacgatc tgttcgtcca gccccaacgc aaacaacatc tccgtgatgc tcggcgccaa
     1441 tgacacgacc cgtgtgggcg ccttggcgac atagagtttt ctgcccgcat cgtcgatgaa
     1501 ggtgcgcggg gtaatgttgg ccatgaacgg catgcccgta aggatgcctt gctgacgacg
     1561 tttcatcacc gtgtgatctt cctgagtctc tccgaatgcg aaggcaaggc aggccagcag
     1621 cacagacgcg accacgccga tgcatgaggc tgcgaaccgg cgactggaga cgattcgaac
     1681 ggcgcgcaaa taaaaaaatc cccagggcca acatggaacc ctgaggattg cacccgcaat
     1741 atcatcctcg cctattcccc agcccacgag ggaataaagg gtctccctca ggcaggtctt
     1801 ctggctcatg gttcatccta ctccccgcgc cttcccactc accgaacggt gaacagtggc
     1861 gcctgcgggt ttcgtcccca tttacagcgg cgggaccgcg agggtttttc accctcttcc
     1921 cttgacccga ggtgtcttga aaagagacaa actttaggag cgcatggccg atcttgtcaa
     1981 gttcaaactt gccgtcacga cgttctatgg agcagcatag gatttcgatg gagcagcata
     2041 ggatattgaa ctgcacaccc agacaccctg cagcccctac gagcgatacc ggagcttcag
     2101 gatgagaatc gccagggtca acaccagggt cacgagattc gccagaataa tgggcaaggc
     2161 gccgagcatg agcccataga tcaaccagag aaagaccccg gtcacgaagg tgagcaacat
     2221 tcccagcgac acgtcctggg cggaacgagt gcgccaggtc tgttgcagct gaggaatgaa
     2281 cgcgatggtg gtgagggtcc ctgccagcaa tccgatgagc gtgacctgat ccatatctgc
     2341 aacctcagtg gagtgagcgg cgcgagcggg agagggcatc ctggtaggga gaattcacct
     2401 cagcagcttg tcatgaccgg tggcgctatg gtagcatccc
//
LOCUS       DAORQN010000220         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1284_length_2432_cov_6.050905, whole genome shotgun sequence.
ACCESSION   DAORQN010000220 DAORQN010000000
VERSION     DAORQN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1284_length_2432_cov_6.050905"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PK614_08900"
     CDS             complement(<1..1332)
                     /locus_tag="PK614_08900"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRB82362.1"
                     /translation="MKILVGISVVILLLIVLIVALPFLIDLNKYQDRYRPLIEEALNR
                     KVELRDIRLTIWPRIGARVGSFVVQDDPAFRTGPFASLSSLDVGVKLLPLLKGTVDVE
                     EITLRDPVITVLKNAQGQLNISTLGAKTPAPPAPSKPEAPAQAPGSPLQILALFAVDR
                     VSIDGGKLSYRDESTPKPTEYTVNNLEFLLTSVHLDESPTVHLGATVQPYNLPVRLDG
                     TFGPLVETLDVKSFTFNLALGKVAVGLKGRAVGGNLDATVNALQIDTADLPIALPLTK
                     PVQLKDVHLTLHALYPVPPDTPPANQVDLTDLGLTLVMGGSAINVKGTATKGLANLTA
                     ASASINSADLPVAIPLTKPVELKDLHVNLRAKYPPKEGAAPLELAEIPNLGLTVAMGS
                     SRIEVKGSVLGGLAKITANSKLINTTDLPIALTLKKPVEVKDLQAAAEMKGQ"
     gene            1550..1639
                     /locus_tag="PK614_08905"
     tRNA            1550..1639
                     /locus_tag="PK614_08905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1584..1586,aa:Ser,seq:gga)
     gene            1894..1993
                     /gene="ffs"
                     /locus_tag="PK614_08910"
     ncRNA           1894..1993
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK614_08910"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2075..>2432
                     /locus_tag="PK614_08915"
     CDS             2075..>2432
                     /locus_tag="PK614_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB82363.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQSHVVQTLMNSIATKRIAHAFLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCNTCANCQEITQGTSVDVVEIDGASNTSVD
                     DVREIRENVKFTPFRGQ"
BASE COUNT          473 a          683 c          797 g          479 t
ORIGIN      
        1 ctgtcccttc atttcggctg cggcctgcag gtctttgacc tccaccggct tcttcagtgt
       61 cagtgcgatt ggtaggtcag tcgtgttgat gagttttgag ttggcggtga ttttcgccaa
      121 tccgccaagc accgatcctt tgacttcgat acgtgagctc cccatggcga cggtgaggcc
      181 aaggttcgga atctcggcaa gttcaagcgg cgcggctccc tccttaggcg gatatttggc
      241 ccggaggttc acgtgaaggt ctttgagttc gaccggcttc gtgaggggga tggcaaccgg
      301 aagatccgcg gagttgatac tggcggatgc cgccgtcaaa ttagccaagc ctttggtggc
      361 cgtgcctttg acgttgatcg ccgatccgcc catgaccaga gtcaatccca gatcggtcag
      421 atcgacctga ttggcgggcg gggtatcggg cggaaccggg tacagggcat ggagggtgag
      481 atgcacatcc ttgagctgta ccggtttcgt cagcggcagt gcgatgggta gatccgccgt
      541 gtcgatctgc agcgcattga cggtggcgtc caggttccct ccgaccgcgc gtcccttgag
      601 cccgacggcc actttcccta atgcgaggtt gaacgtgaac gacttcacgt ccagggtttc
      661 aacgagtgga ccgaaggtgc catcgagccg gaccggcaag ttgtagggct gcaccgtcgc
      721 acccagatgt acggtggggc tttcgtcgag gtggaccgag gtgagcagaa actcaagatt
      781 attgacggtg tactcggtcg gtttcggggt agactcgtca cgatacgaca gttttccccc
      841 gtcgatcgag acccggtcca ccgcaaagag cgccaggatt tggaggggac tcccgggcgc
      901 ctgcgcgggg gcctcaggct tcgaaggagc cggcggagcc ggtgttttgg caccgagggt
      961 cgagatgttg agttggccct gggcattttt caggaccgtg atgaccgggt cacgcagggt
     1021 gatctcctcg acgtcgactg tgcccttgag cagcggtagc agcttcacgc cgacatccag
     1081 tgaagagagt gatgcgaagg ggcctgtgcg aaaggccgga tcgtcctgca cgacaaagct
     1141 gccgaccctt gcgccgatgc gtggccagat ggtcagccga atgtcccgga gttcgacctt
     1201 ccggttgagg gcctcttcga ttaaggggcg gtaccggtcc tgatatttgt tcagatcgat
     1261 gaggaatggt aacgcgacga tcaggacgat gagcagcagg atcacgacac tgatcccgac
     1321 caggattttc atcacgtacc tccgctgaaa tagtcccgca gtataggaat gcgcgcagga
     1381 gtggtcaatg gtcgcatcct gagggcagtc gccacggggc cctcgggggc tctcgccttc
     1441 gaagccggac ttcccatcgt cgtcgcagtg gcatgtttgc aggctccggc ttaccttgcc
     1501 gcaccaaaac ctgcgtgata agatgcgccc cttgaagtcg tccgatcgcg gagaggtgcg
     1561 agagtggacg aatcgacatg cttggaaagc atgcgtctcg gcaacgggac cgtgggttcg
     1621 aatcccaccc tctccgccat cgaacagcgc catgcaaggg tgagaaggcg tgcgagttgg
     1681 aattgccgtc gcgaactgat gcggtgggat tcgaacgagg gggatcgcgg gggagaggct
     1741 cccctgcgtg gggagacacg agggggcggg ccccctcagt gggagcgcgt gagcgtgact
     1801 tcgaatgccc accctctccg ccaaacagag ctagctcgac cgccagccat tctatgactc
     1861 taggcgagcg gagaagatcc tttagtgtaa cggggccggg ccctgtgcaa cagaaccctg
     1921 tgaaccccgc caggtccgga aggaagcaac ggtaagcggt tcgttctgtg tgccgcagga
     1981 tcacctggcc ccacttcttc gatgacatct cgcgcgacgc gtcaggcgcc gccagcacgg
     2041 acgagcacca ccgccccgac acgatcgacg caacatggac taccaagttt ccgcgagaaa
     2101 ataccggccc gggacgtttg acgatgtcat cgggcagtcc cacgtcgtgc agacgttgat
     2161 gaactcgatc gcgacaaagc ggattgccca tgcgttcctc ttctccggaa cccgcggcgt
     2221 ggggaaaacg acggtcgccc ggattttggc caaagcgctg aattgcgagc aggggccgac
     2281 gggaacgccg tgtaatactt gtgcgaattg ccaggagatt acgcagggca cgtcggtgga
     2341 cgtggtcgag atcgacgggg cctcgaacac cagcgtggac gacgttcgcg agattcgcga
     2401 gaacgtcaag tttacgccgt tccgggggca gt
//
LOCUS       DAORQN010000221         2417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1289_length_2417_cov_6.632515, whole genome shotgun sequence.
ACCESSION   DAORQN010000221 DAORQN010000000
VERSION     DAORQN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_2417_cov_6.632515"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK614_08920"
     CDS             <1..690
                     /locus_tag="PK614_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB82364.1"
                     /translation="RRNRPPMGAEPGSYVALIVRDTGHGMDEDTQSHVFEPFFTTKEK
                     GKGTGLGLSTVYGIVKQSGGCIEVESKPGRGATFKIYFPRVESAATETESVAVGGDSV
                     RGRETVLLVEDEPGVRRLVNETLRLHGYTVLEARHGIEALLTGAKHAGPIHLLLTDVV
                     MPQMSGPEVAEKLATVRPEVKVLYMSGYPDHPAFSKGGIDTEHSFLQKPFTPTTLAQK
                     VREVLDGSKVA"
     gene            811..1650
                     /locus_tag="PK614_08925"
     CDS             811..1650
                     /locus_tag="PK614_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRB82365.1"
                     /translation="MSEGRTASVPKGQEQEIDRYRIAREPFYAEVRGEVGLFTIAAQS
                     RMPVMLKGPTGCGKTRFVQHMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWMD
                     GPLTLGVKQGAIVYLDEIVEARKDTPVIIHPLSDDRRLLPIEKKGQVIEAVDDFMLVI
                     SYNPGYQSILKDLKQSTKQRFMAIEFDYPLPDVESRVVAHEAGVSLEVAQRLVKIAEK
                     VRNLKNHGLEEGVSTRLLIYAATLIRQGVPADQACDVAIARPITDDPDMLRSIIEVVK
                     AIF"
     gene            1716..2033
                     /locus_tag="PK614_08930"
     CDS             1716..2033
                     /locus_tag="PK614_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRB82366.1"
                     /translation="MRERQATEREQAVRATSEGVTISVHVQPKASRSECAGLHGHAVK
                     IRIAAPPADGAANDELCRFLARRCDVPLSAVHILSGAGSRQKRVLVKGPSVEQVRAQL
                     HLE"
     gene            2181..>2417
                     /locus_tag="PK614_08935"
     CDS             2181..>2417
                     /locus_tag="PK614_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82367.1"
                     /translation="MKASRSSFCLIFLVTIGLVACAGPEPILKSNTRVQLYGKDQAAR
                     DAAVCGEKAERAGLQHGTNRSGNAGAGATLGIVGG"
BASE COUNT          490 a          669 c          804 g          454 t
ORIGIN      
        1 cgcaggaacc gtccgccgat gggcgccgag cccggttcct atgtggcgtt gatcgtgcgc
       61 gataccggcc acggaatgga cgaggatacg cagtcgcacg tcttcgagcc gttttttacg
      121 accaaagaga agggcaaggg cacggggctc ggtctttcga cggtctatgg catcgtcaag
      181 caaagtggcg gctgtatcga ggtcgagagc aagccgggac gcggggcgac ctttaagatt
      241 tacttcccgc gtgtggagtc ggccgcaacg gagaccgaat ccgtcgcggt cggaggggac
      301 tcggtcagag ggcgcgagac ggtgttgttg gtggaggatg agccgggtgt ccgacggttg
      361 gtcaatgaaa cgttgcgctt gcacggatat acggtgctcg aggcccggca cgggattgaa
      421 gcgttgctca ccggcgcgaa acatgccggt ccgatccatt tgctgctcac cgacgtggtt
      481 atgccgcaga tgagcggacc ggaagtcgcc gaaaaactcg cgacggttcg tccggaggtg
      541 aaagtgctgt acatgtctgg ctatccggat cacccggcct tttccaaggg agggatcgat
      601 accgagcatt ccttcctgca gaagcccttc acacccacga ccctggcgca aaaggttcga
      661 gaagtgctcg atggatcgaa agtcgcctga ccgtaccgcg caagagtgcg gggctttccc
      721 tgtcttcagt cggctgcttg aacttcccgc tcgtttccgt ttatcgtggc gcaccatgca
      781 ggttattcaa ttcaatgtag gagtgtcgga atgagcgaag ggcgaacggc atcggtgccg
      841 aaagggcagg aacaggagat cgaccggtat cggatcgcgc gcgagccgtt ttatgccgaa
      901 gtgcggggcg aggtcgggct gttcacgatt gcagctcaga gtcgcatgcc ggtaatgctg
      961 aaggggccga cgggatgcgg gaagacgcgc ttcgtccagc acatggccta tcgcctgggg
     1021 cgtccgctca ttaccgtggc ctgccatgaa gatctcacgg cctccgatct cgtcggtcgg
     1081 tatctgttga aggggcagga aacggtctgg atggacggcc cgctgaccct cggcgtcaag
     1141 cagggggcca tcgtctacct ggacgagatc gtcgaagcca ggaaagacac ccccgtcatt
     1201 atccatccct tgagtgacga ccggcgcctg ctgccgatcg agaagaaggg gcaggtcatt
     1261 gaagcggtcg atgacttcat gctcgtgatt tcctacaacc ccggctacca aagtattctg
     1321 aaagatctca aacagagcac caagcaacgg ttcatggcga tcgagttcga ctaccccttg
     1381 cccgatgtcg agagccgggt ggtggcgcat gaagccggcg tgagcctcga ggttgcgcag
     1441 cgtctcgtca agattgcgga gaaagttcgc aacctcaaga atcacggact ggaagagggc
     1501 gtgagcaccc gactgctgat ttatgcggcc acattgatca ggcagggtgt accggccgat
     1561 caagcctgtg atgtcgccat tgcccgtcct atcaccgatg atccggacat gttgcgcagc
     1621 atcatcgaag tcgtgaaggc gatattctga ggcatccgcc cactgcgacg cggtgaggcg
     1681 gtgtttgcag gagctcggcg accagccgca cccccatgcg agagcgtcag gccactgaac
     1741 gcgaacaggc cgttcgcgcc acatccgagg gcgtgaccat ttcagtccat gttcagccga
     1801 aagcctcccg cagtgaatgt gcaggtctgc atgggcacgc ggtgaagatt cgcattgccg
     1861 cgccgccggc tgacggcgcg gcgaacgacg aattgtgccg gtttctcgcc cgtcgctgcg
     1921 atgtgccgtt aagcgcggtt cacatcctga gcggtgcggg aagtcgccag aaacgcgtct
     1981 tggtcaaggg gccatcggta gaacaggtgc gtgcacaact ccacctggaa tgacatgagc
     2041 ggcgggtaga tcgcgagcct tgctgtccgg aggcgtcgtc cagggaaaag catgcgaggt
     2101 aatccgatga ctaggacagg gcagacaggt tcaattcggc ggggccaaac ccggtgttcg
     2161 gcatgcagcg aggtgcggat gtgaaggcgt cgcgatcgtc attctgcctg attttcctgg
     2221 tcacgatcgg tctcgtggcc tgtgccgggc cagagcccat cctgaaatcc aacactcggg
     2281 tacagctgta tggcaaagac caggcggcac gtgacgcggc ggtgtgcgga gagaaagcgg
     2341 aacgcgccgg tttgcagcac gggaccaatc ggagcggaaa cgccggcgcc ggcgccacgt
     2401 tggggattgt cggcggg
//
LOCUS       DAORQN010000222         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1292_length_2413_cov_7.713740, whole genome shotgun sequence.
ACCESSION   DAORQN010000222 DAORQN010000000
VERSION     DAORQN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_2413_cov_7.713740"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK614_08940"
     CDS             <1..448
                     /locus_tag="PK614_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HRB82368.1"
                     /translation="VVTCRDEADGLWVECFATVGVTNAVRAGEPPPREEADSTVRRAG
                     TINLMVVTNACLAVPALVCAVQVVTESKTGVLRDHAVPSWTGRSGATGTGTDAVVVAC
                     ALRGSGPRSDYAGTHTTIGSLIGRVVAECLTQGLARATEWAATHRG"
     gene            465..2036
                     /locus_tag="PK614_08945"
     CDS             465..2036
                     /locus_tag="PK614_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRB82369.1"
                     /translation="MPPPSRAKAIAILGTGSDVGKSMITSGLCRLLYRAGFRVAPFKA
                     QNMSLNSFVTPEGGEIGRAQALQAEACGIPPHVDMNPILLKPESDSRSQIIVQGKVFA
                     TREARAYFARSRDSELFRAVECSYNRLALQYEVMVIEGAGSAAEVNLRDRDLVNWPVV
                     EMADAKVVLVGDIDRGGVFAQIIGTLDLITPEERDRVCGIVINKFRGDAALFADGVRF
                     LTERTGIPVLGVLPFLRDLALDQEDSLDVDIRRQVAFQRDCINLAVILLPHMSNFTDF
                     NALAGEPDVALRYAATPEEAAGADAILLPGSKTTLADLAYLRGKGFESLLHGHVHRGG
                     EVVGICGGYQMLGRRIADPDAVESGGSGEGLGLLQVTTVLMREKITEQVEGRALYLGG
                     PAAPPVRGYLIHMGRTDRHGHRACFELQGKEPSPAGPRDVTERDDAWLDGAMSDDGLV
                     WGTYIHGLFDEPLCRRAWLNRWRIRKGLPAADMALSQQVNRQRSGAMDRWADHVEQHL
                     DLTPILALLRQGGPV"
BASE COUNT          448 a          675 c          785 g          505 t
ORIGIN      
        1 ggttgtgacg tgccgtgatg aggcagatgg cctctgggtc gagtgttttg ccaccgtcgg
       61 tgtgaccaac gcggtgcggg caggtgagcc gccgccgcga gaagaagctg acagcacggt
      121 ccgtcgagcc gggacgatca atctgatggt ggtcaccaat gcctgcctcg ccgtgccggc
      181 cctggtctgt gcggtgcagg tggtgacgga gagcaagacc ggagtgctgc gtgaccatgc
      241 ggtgcccagc tggaccggtc gttccggcgc gaccggcacg gggacggacg ccgtggttgt
      301 cgcctgtgcc ctgcggggga gcggtcccag gtcggactat gccggaaccc acactacgat
      361 cggatccttg atcggacggg tcgtcgccga gtgcctcacg caaggtctgg cgcgcgcgac
      421 agagtgggcg gcgacacatc gcggctaaac ggttgtcaat tcctatgcct cctccttctc
      481 gtgctaaagc cattgcgatt ctcgggaccg gctcagacgt cgggaaaagc atgatcacgt
      541 cggggctctg ccggctgttg tatcgcgccg ggttccgcgt cgcgcccttc aaggcgcaga
      601 atatgtcgtt gaactccttt gtcacgccgg agggcggtga gatcgggcgt gctcaggctt
      661 tgcaagccga agcctgtgga attccccccc atgtggatat gaatcccatt ctgctcaagc
      721 cggaatccga ttccaggtct cagatcatcg tgcagggaaa agtatttgcc acgcgcgagg
      781 cccgggccta ttttgctcgg agccgggact ctgaattgtt tcgggcggtg gaatgcagtt
      841 acaaccgcct cgccttgcag tatgaggtca tggtcatcga aggcgccggc agcgctgcgg
      901 aggtcaatct gcgagatcgc gatctggtga actggccggt cgtcgagatg gccgatgcca
      961 aggtggtgtt ggtcggggat atcgatcgcg gcggcgtgtt cgcacagatt atcggcacgc
     1021 tcgatctcat cacgccggag gagcgtgacc gggtctgcgg gatcgtcatc aacaagttcc
     1081 ggggagacgc ggcgctcttt gccgacggcg tccgtttttt gacggagcgg acagggattc
     1141 ctgtcctggg cgtcttgccg tttttgcgcg acctggcact cgatcaagag gacagtctgg
     1201 acgtcgatat ccgacgacag gtcgcgtttc agcgtgactg tatcaacctg gcggtgatcc
     1261 tcctgcccca catgagcaac ttcaccgatt tcaatgcgct ggccggtgaa ccggatgtgg
     1321 cgttgcgata tgcggcgacc cccgaagaag cggccggggc tgatgcgatt ctgcttcccg
     1381 gttccaaaac cacattggcg gacctcgcct atctgcgagg caaggggttc gaatcactcc
     1441 tacatggtca tgtccaccgc gggggagaag tggtcggcat ttgcggcggg tatcaaatgt
     1501 tggggcgccg gatcgccgat ccggatgcgg tcgaatcagg cgggagcggc gaggggctcg
     1561 gcctgctgca ggtgacgacg gtgttgatgc gggagaaaat caccgagcag gtcgaagggc
     1621 gtgccttgta tctcggtggg ccggcggcgc caccggttcg agggtacttg attcacatgg
     1681 gacggacgga tcgccatgga catcgagctt gcttcgaact gcagggcaaa gaaccgtcgc
     1741 ccgcaggacc acgcgacgtc accgaacggg atgatgcgtg gctcgacggc gcgatgagcg
     1801 atgacggtct ggtctggggg acctacatcc atggcctgtt tgacgagcca ctctgtcgac
     1861 gtgcatggtt gaaccggtgg cgtatcagaa aagggctgcc agccgccgac atggccttat
     1921 cgcaacaggt gaaccgacag cgatccggtg cgatggatcg atgggccgat cacgtcgaac
     1981 aacatcttga tctcaccccg atcctggcgc tccttcgtca aggtgggccg gtctaggcca
     2041 gccgcaatcc tcttttcgtc gaagttctga agggactttg acgcttccct cgaaaagcga
     2101 ccaaactttg gcgctccgac ttccatcgtg aacaattacg cacccatgtc agcctagctg
     2161 accatagtgg gtatagtaga ccatccacac ataatcatcc gcaaaatcgc cgacttcatc
     2221 ggttcgaatt ctttccgctc cgcaatccta actaattgtt ttgtttggca ccgatcgtgc
     2281 acaacgtctg cctgggctgc agggtcctga cgtggttcca tgcgtcgatg aaattgtcaa
     2341 cgaagaattt tgcgctatct ttgacaaggg attggctgac ttaggggaat tgggttgacc
     2401 gggcgctacg agg
//
LOCUS       DAORQN010000223         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1303_length_2390_cov_8.722912, whole genome shotgun sequence.
ACCESSION   DAORQN010000223 DAORQN010000000
VERSION     DAORQN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_2390_cov_8.722912"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /gene="ftsY"
                     /locus_tag="PK614_08950"
     CDS             <1..844
                     /gene="ftsY"
                     /locus_tag="PK614_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRB82370.1"
                     /translation="LLGRAADPALLDELEVALISADLGMPVVERVMGQLRAQMRGGNF
                     SSADKVRDLLRQSVLDILLPTQSASMEQLVAQGPRPFVILAVGVNGVGKTTTVAKLTQ
                     RFRQQGKKPLLVAGDTFRAAAIDQLQVWADRIEVDVIRHRPGADPAAVAYDGMTAAKA
                     RGSDVVLIDTAGRLHTKTNLMDELRKIKRVVAQECQGAPHEVLLVLDATVGQNAIAQA
                     RQFHEAVGVTGIALTKLDGTARGGIVVAIADTFKIPVRLIGVGEAVDDLQDFDAKAFV
                     DALF"
     gene            1048..1836
                     /locus_tag="PK614_08955"
     CDS             1048..1836
                     /locus_tag="PK614_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB82371.1"
                     /translation="MMSTLLVIDDDRLHCDLLQMALARHGYQVSIATSGREGVVLFQQ
                     LRPLVTLLDLRMPEMDGLAVLKEIRTHDPRAGVIMLGGGATEELENQARELRVTDFLR
                     KGLSLDVLIGAVHRVAQQARRQASSEQSRAGEEADQLPDEHILVVDDDVMARDLLVRF
                     LSLRGYHVRAAQDGREALRLIGESVPDLLILDLAMPEMNGVEVLRALAARDYVGRTII
                     LSGHQNDPLLAEAWALGPQEVLDKPIDLERALMAIQLVMVCREC"
     gene            1870..>2390
                     /locus_tag="PK614_08960"
     CDS             1870..>2390
                     /locus_tag="PK614_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82372.1"
                     /translation="MNGLYRLIRFSLLAICLLPAPAVADSVAPPPTIEHHQLALELSP
                     DSHRLIATDRVTVAGGSPGQELAFSLAPTLLLERVEDLTHCQDPCEFALSVPFARDRS
                     SSQAESQRILLKQPPANQAGGAIRLNFVYHGLIDDPPRDPLHLRFVTPSETAGHIGPE
                     GVYLSSESQWYPDL"
BASE COUNT          432 a          662 c          783 g          513 t
ORIGIN      
        1 actcctgggg cgggcggctg atccggcgct gctggatgag ctggaggtgg ccttgatcag
       61 cgcagatctg ggcatgccgg tcgtggaacg tgtgatgggg cagttgcgtg cccagatgcg
      121 gggcggtaat ttctcgtcgg ccgataaagt acgagacctg ctgcggcaat ccgtgctgga
      181 tattcttctc ccgacacaat ctgcctcgat ggagcaattg gtggcacagg ggccgcgtcc
      241 ctttgtgatt cttgccgtgg gggtcaacgg tgtcggcaag accacgacgg tggcgaagct
      301 gacgcaacgg tttcgacaac aggggaaaaa accattactc gtggccggtg ataccttccg
      361 cgcggcggcg atcgatcaat tgcaggtctg ggcggaccgc atcgaggtcg atgtgatccg
      421 ccatcgaccg ggtgcggatc cggctgcggt cgcctacgac gggatgacgg ctgcgaaagc
      481 gcgcgggtcg gacgtggtgt tgatcgatac cgccgggcgg ctgcacacga aaacgaatct
      541 catggacgaa ttgcggaaga tcaagcgggt ggtcgcgcaa gagtgtcagg gagcgccaca
      601 tgaggtattg ttggtgttgg atgcgacggt cggccagaat gccatcgccc aggcgagaca
      661 gttccacgag gcggtcgggg tgactggaat cgccctgacc aagctggatg gaacggcgcg
      721 tggtggcatc gtggtggcca ttgccgacac cttcaagatc ccggtccgct taatcggcgt
      781 cggggaagcc gtcgatgacc tccaggactt tgacgccaag gcgtttgtcg acgcgttgtt
      841 ctagcccgca tgattcgtga cggtttcgtc acgaactctc ttgaagcatg cggactggcc
      901 tttcctgacg gacggcggtc cgtgatccac tccagacgtt ttcattcaat ttcttctcat
      961 cttgtcgtgc ccatgctagg ctcctgctaa cgggctgcta ccgtctgcgg ccctgcgcga
     1021 ctggttgtga cgcacaaacg agataagatg atgagcacgt tactggtgat cgacgatgat
     1081 cggttgcact gcgacttgtt gcagatggcg ctggcccgtc acgggtatca agtcagcatc
     1141 gcgacgagcg ggcgtgaggg tgtcgtctta tttcaacagc ttcgcccgct ggtgaccttg
     1201 ctcgatttgc gcatgccgga aatggacggt ctggcggtac tcaaggagat ccgaacgcat
     1261 gacccccgtg ccggggtgat tatgctgggt ggcggcgcga cggaggaact ggagaatcag
     1321 gcgcgcgagt tgcgcgtcac ggatttcctc cgaaaggggc tgtcgctgga tgtgctcatc
     1381 ggggccgtcc atcgtgtggc gcaacaggca aggcgacagg cttcgtccga gcagtcccgt
     1441 gccggagaag aggcggacca gcttcccgac gagcacatcc ttgtggttga tgacgatgtg
     1501 atggcgcgtg atctgctggt acggtttctc agcctgcgcg gataccacgt tcgcgccgcg
     1561 caagacggcc gtgaagcatt gcggctgatc ggagaatcgg tgccggacct gctgatcctg
     1621 gatctcgcca tgccggagat gaacggcgta gaggtcttgc gggcgcttgc ggcgcgagac
     1681 tatgtcggca ggacgatcat tttgagcgga catcaaaacg atcccttact ggccgaggcc
     1741 tgggcgttgg gtccgcaaga ggtcctggac aagccgattg atttggagcg ggcgttgatg
     1801 gctatccagc ttgtgatggt ctgtcgggag tgttaatctt caccgtccac ctcagggtgc
     1861 cggtgaaggg tgaatggttt gtatcgcctc atccgtttca gtctcctcgc aatttgtctc
     1921 cttcctgcgc ccgcagtcgc tgattctgtt gctccccctc cgacgattga acaccatcag
     1981 ctcgcgctcg agttgtctcc tgattcccat cgactgatcg cgacggatcg agtgaccgtt
     2041 gcgggaggat ctcccggaca ggagctggca ttttcccttg caccgacgct cttgttggaa
     2101 cgggtcgagg atctcacgca ttgtcaggac ccctgtgagt tcgccctctc cgttccattt
     2161 gcacgggaca ggtcgtcaag tcaggcggaa tcgcagcgca ttctcctgaa gcagccgcct
     2221 gccaaccagg cgggcggagc gatacggctc aactttgtct accatgggtt gatcgatgat
     2281 ccgccccgag atccactcca tctgcggttt gtcacaccga gcgaaacggc cgggcacatc
     2341 ggcccggagg gggtatatct cagcagcgag agccaatggt acccggacct
//
LOCUS       DAORQN010000224         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1304_length_2387_cov_52.369640, whole genome shotgun sequence.
ACCESSION   DAORQN010000224 DAORQN010000000
VERSION     DAORQN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_2387_cov_52.369640"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..683
                     /locus_tag="PK614_08965"
     CDS             108..683
                     /locus_tag="PK614_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRB82373.1"
                     /translation="MRKLIGGMKVSVDGKITGSDDYADWVQGWSDDYGLTPQVDACVL
                     GAGMYTGYEQYWTAIQNDPDKPLPMTGTLPTPGEIEWARFAAETPHYVLSSTLTSVYW
                     PKTSIVRRIEDIAALKQQSGKNIYLVGGARTTASLIDAGLVDELRLIVYPLIAGSGKA
                     LFAEMASRHRLELQKVQQLQDGRVHLIYRIG"
     gene            693..1124
                     /gene="tsaA"
                     /locus_tag="PK614_08970"
     CDS             693..1124
                     /gene="tsaA"
                     /locus_tag="PK614_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRB82374.1"
                     /translation="MALATDMTRFEVKPIGWVSSDLKGLDSVPRQAEEGTPEVWLVFD
                     LELLEGLQALQLGDRILVLTWLDRTRRDMLRVHPRGDAPRPLEGVFRTHAPHRPNPLG
                     PHSVEITAIEDVRLRIRHLEALDGTPIIDVKPILNAEVSRR"
     gene            1219..1467
                     /locus_tag="PK614_08975"
     CDS             1219..1467
                     /locus_tag="PK614_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82375.1"
                     /translation="MISTIFVNLPVQDLDKSIAFLGRLASRSTPQCMQETAACMVMGD
                     DIDAMLWTHEKVKEIADKALKVDAQNAYAPKALSRSLE"
     gene            1521..2015
                     /locus_tag="PK614_08980"
     CDS             1521..2015
                     /locus_tag="PK614_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB82376.1"
                     /translation="MARNPIAKHTICLRYDNNVEDAARFYPTTFPDSAVCRSHLEQEV
                     SQMPLMCRIITVVLIFVWSMWPSLGLAGGEEPAQQPSARERLNQVLSPEGGIQVYKDQ
                     TGAVESTINLPNGERIITVQPPQGAGLNLGPPLQLNNPILQFPPQPTVPAQPPTPEFP
                     QRAR"
BASE COUNT          609 a          607 c          669 g          502 t
ORIGIN      
        1 gtctcaagca actcattgaa actgggagcg gtaaacctgc tccgcgagat atcaagatcg
       61 ataattggaa ctaactcgca cgagattcta ttgcggagga tgatgatatg cggaagttga
      121 ttggtggaat gaaggtatct gtcgatggga agatcacggg ttctgatgac tacgcggatt
      181 gggtacaagg gtggtcggat gactacggcc taacgccaca agtggacgcg tgcgtgcttg
      241 gggccggtat gtatacaggc tacgagcagt attggactgc gatacagaat gacccggata
      301 agccccttcc aatgactggc acgttgccaa cgccgggtga gattgaatgg gctcgtttcg
      361 cggcggagac cccacattac gtgttatcga gtaccctgac ttcagtatat tggccgaaaa
      421 caagcatcgt tcggagaata gaagacattg ctgctcttaa acagcagtcc ggaaagaata
      481 tatatctcgt cggaggtgcg cgaacgacgg cgagcttgat cgatgccgga ttggtggatg
      541 aactccgcct cattgtatac ccgctgatcg cgggtagcgg gaaagcgctc ttcgcagaga
      601 tggcatctcg tcataggctt gagctacaga aggttcagca gcttcaggat ggacgtgtac
      661 acctgatcta tagaataggg taagggagca agatggccct ggcaacggac atgacacgtt
      721 ttgaagtgaa acccattgga tgggtttcat cagacctgaa gggccttgac tcggtccctc
      781 ggcaagcaga ggaaggcaca cccgaggtct ggttggtttt tgacctggag ttgttggagg
      841 gcctccaagc tctgcagctc ggtgatcgca ttcttgtcct cacctggctc gatcggaccc
      901 gtcgggatat gttgcgcgta catcccagag gagacgcccc tcgacccctg gagggcgtct
      961 tcaggacaca cgcgcctcac cgaccgaatc cgcttggccc gcactctgta gaaatcaccg
     1021 cgattgaaga cgtacgattg cgaatacgcc atctcgaagc gctggatgga acgccgatca
     1081 tcgacgtgaa acccatcctc aacgcagaag tcagccgacg ttagccagag ggcacgcgcc
     1141 gagtagaaac aaccacgagg atcggaagcg cttcatcata actcgctcga atgactcgat
     1201 ggcaaaaggc ggacagaaat gatcagtaca atattcgtca atctccccgt gcaggacctc
     1261 gacaagtcga tagcgttttt agggcgattg gcttctcgtt caacccccca gtgtatgcaa
     1321 gagaccgcgg cttgcatggt catgggcgac gacatcgatg ccatgctgtg gacgcacgag
     1381 aaggtgaagg agatcgcaga caaggcgttg aaggtagacg cacagaacgc ctacgcgccg
     1441 aaggcgctat cgagaagctt agagtgaaag agaacttagt agaatcaaat agtacaacaa
     1501 atagctgaaa ggaagacaac atggccagga atccaattgc gaagcatacg atctgtctgc
     1561 ggtatgataa caatgtcgaa gatgccgcgc ggttctaccc gacgaccttt cccgacagcg
     1621 ccgtgtgtag atcccacctt gagcaagagg tcagtcaaat gccactcatg tgtaggatca
     1681 tcaccgtcgt cctcatcttc gtgtggagca tgtggcccag cctggggctc gccggtggag
     1741 aggaaccggc tcaacagcca agtgcgcggg agcggttgaa ccaagtgtta agtcctgaag
     1801 gcggcattca ggtctacaag gaccagacag gcgccgtcga aagcacgatc aacctgccca
     1861 acggtgaacg aattatcaca gtccagccac cgcaaggtgc cggactcaat ctggggcctc
     1921 cgcttcaatt gaacaatcct atcctccagt ttcctcctca gcccactgtt cccgctcaac
     1981 cacctacgcc agaatttcca cagagggcac ggtagacgtc agtgatcgcc tttcgtcttg
     2041 ttgaattgat tgaacgagtc cggcaactga taacctgcag atagtccagt ggcctgatgg
     2101 catacatccg tggaaagtct tccggcacga attgttccag gaggggtctc acgtctcggg
     2161 taaccttttc accttatggg tgtccacgtt cgctcagtgc cctaaaaaga gagactaaat
     2221 gagagacagt tgtatggaac cacccgtcgg aaagaactgt attccatgag ttaaaatggt
     2281 agggttacag ccgtaagaat gatcgaagtg ggatcacata attggacatt tacccgctca
     2341 gagctcgtgc agctgtacat gacggtgacc ccaccttggg attcgtg
//
LOCUS       DAORQN010000225         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1313_length_2360_cov_8.938395, whole genome shotgun sequence.
ACCESSION   DAORQN010000225 DAORQN010000000
VERSION     DAORQN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1313_length_2360_cov_8.938395"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PK614_08985"
     CDS             <1..890
                     /locus_tag="PK614_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB82377.1"
                     /translation="HIYAQSQELARVDSTVLIEGETGTVKELVARALHQASARHKGPF
                     IAANCAGLTDSLLGSQLFGHKKGAFTGAIDDQQGLFEAAQGGVLFLDEIGDIPHNVQT
                     NLLRVLQEKEVTRLGETRPRKVNVRVLTATHHNLSQDVAKGTFRADLLYRIRVSRIQL
                     PPLRERKEDIPLLVASFLTEGRASMGKVIHRASPAAMAALMEYHWPGNVRELKSTIEC
                     AMIHCKGDTLEATDLPPELRQGPTDHPSEVVLHGDERSRFVAALGQARGNRTKAARLL
                     GMSRATFYRRLSELDLPTS"
     gene            1158..1475
                     /locus_tag="PK614_08990"
     CDS             1158..1475
                     /locus_tag="PK614_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB82378.1"
                     /translation="MDKLIPYSRPRPDAPTAREREILTHIWAGLTSQEIATRLQIAPK
                     TVESHRANLLRKFRATNSAQLLRLALLEGILHMPSSTSTGPSQTAADAQSITAVRKLT
                     NLK"
     gene            1852..>2360
                     /locus_tag="PK614_08995"
     CDS             1852..>2360
                     /locus_tag="PK614_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82379.1"
                     /translation="MRRLGTLCFALLVMALAASIARAEAPAPADATPAAPALDAVTLK
                     DGTVIYGQVLGMIADELHIKTSFGPTAGDDIVKIMWPNVAKLVVNRPVPFSLKEGTTV
                     VGTAQPGEPGTMILKAAPTGTPMAIPLDAVIGMNQPAVIYTGALQAGFSQTTGNSHLR
                     NGSLLGELSA"
BASE COUNT          549 a          772 c          643 g          396 t
ORIGIN      
        1 cgcatatcta cgcacagagt caggagctgg cccgtgtcga ctccacggtc ttgatcgaag
       61 gcgagaccgg tacggtgaaa gaattggtgg cccgcgcgtt acaccaggcg agcgcacgac
      121 ataaagggcc cttcattgcg gccaactgcg ccgggctcac ggattcgctg ctgggcagcc
      181 aactcttcgg ccacaagaaa ggcgccttta ccggagccat cgacgaccag caggggctct
      241 tcgaggccgc gcagggcggg gtcttgttcc tcgacgagat cggcgatatc ccccacaacg
      301 tccaaaccaa cctgcttcgc gtactgcagg agaaggaagt cactcgactc ggtgaaacca
      361 ggccgagaaa agtgaacgtg cgcgtgttga ccgcaaccca ccacaatttg agtcaggacg
      421 tggcgaaagg aacctttcgc gccgaccttc tctatcggat tcgagtctca cggatccagc
      481 tcccgccgct gagggagcga aaggaagata ttcccctcct cgtcgcctcc ttcctcaccg
      541 aggggcgggc cagcatgggg aaagtcatcc atcgcgccag cccggcagcg atggcggcac
      601 tgatggagta ccattggccc ggcaatgtgc gggaactcaa gagcacgatc gagtgcgcga
      661 tgattcattg caagggcgac acgctcgaag ccacagatct tcctcctgaa cttcgtcagg
      721 ggccgacaga ccatccctcc gaagttgtgc tgcacggaga cgagcgcagt cgtttcgtgg
      781 ccgccctcgg acaagcccgt ggcaaccgaa caaaggccgc gcgcctcctc ggaatgagcc
      841 gcgccacgtt ttaccgccga ctgagtgaac tcgatctccc aaccagctaa tccgtcccgc
      901 agccactagc ccccactcac tgtctcgata tgtctcgcac gacacagcgc gagacaacgg
      961 cgttcgtctc atcagcgcat aatcatggca gattcttaag ggtcctgagc cgagacccca
     1021 gatttcatcg tcgcgcggca aaacacacag cttatggcat agagcttgat aggagagtac
     1081 ccgcgaaaac cactgcgtgc aaaaccttga agacgggcgt cgcagcagac gtatcgaagc
     1141 gatcgcaagg agaaccgatg gacaagctca taccctactc acgaccacgg ccggatgcgc
     1201 cgacggcaag agagcgcgaa atactcactc acatttgggc cggcctgacg agtcaggaaa
     1261 ttgcgacgcg gctacagatc gcccccaaaa ccgtggaatc acaccgtgcc aatctcttga
     1321 gaaaattccg cgcgacgaac tcagcacaac tgcttcgcct ggcactgctt gagggcatac
     1381 tccacatgcc gtcatcgact agcaccggcc catcccagac cgcggcggac gcgcagagca
     1441 tcacagctgt acggaagctg acgaacctca aatgacgctc tcagctccca cacgtatgac
     1501 tggcgcaccg gtgcgcatgt gcacagcgac cgcaccggaa gcctcagact cacaagcctg
     1561 tcgcatagtg aaccactact ccgtgccatc ggccctgctg cgcccatgcc ctgaacagac
     1621 ggtatgacac gccgttatct acgtatactc agcgggacga gccattcatg cctgtggaaa
     1681 ggccgtcctg cgctaggccc acaattcaag ccgaacagcg acacgacgga tttctcgcgc
     1741 caaaatgttg atctcctacg cagaatcaat tatacatcgc gaacatttct cactgatcag
     1801 gcaaccgaca cactgttgcc gttcgcaagc cccaggagga ggacctgaac gatgcgacga
     1861 cttggaaccc tctgttttgc gctacttgtc atggcgctcg ccgcctcaat cgcgcgagca
     1921 gaagccccgg caccggccga tgcgacaccc gcggcgcccg cgctcgatgc cgtcacgctc
     1981 aaggacggca cggtgatcta tgggcaggtc cttggcatga tcgcggacga actgcacatc
     2041 aaaaccagtt tcgggccgac cgccggtgac gatatcgtca agatcatgtg gccgaacgtc
     2101 gccaagctcg tggtcaatcg tcccgtcccc ttcagcctca aagaaggcac gacggtagtg
     2161 ggtacggcac aaccaggcga gcccggcacc atgatcctga aagccgcccc cacagggaca
     2221 ccgatggcca ttccactcga tgcggtcatc ggcatgaacc agcctgccgt gatctatacc
     2281 ggcgccctcc aggccggatt ttcacaaacg accggcaaca gccacctacg aaatggcagc
     2341 ttgctcgggg aactgtcggc
//
LOCUS       DAORQN010000226         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1316_length_2358_cov_4.597916, whole genome shotgun sequence.
ACCESSION   DAORQN010000226 DAORQN010000000
VERSION     DAORQN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_2358_cov_4.597916"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK614_09000"
     CDS             <1..436
                     /locus_tag="PK614_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB82380.1"
                     /translation="ALVLVSTTDGAMILDKDIPTIGAPRDYHEVSKNMIVESFPPGTQ
                     TKTYQPLLDRIPTWNATVIREALTSMSQFNVHGQLTRISVPTLIMVGEKDDVATPAIA
                     KGIQAQISGAKLVEFNTGHFMMAEDPDRFRTVLEDFLKGLPK"
     gene            548..958
                     /locus_tag="PK614_09005"
     CDS             548..958
                     /locus_tag="PK614_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82381.1"
                     /translation="MHVSRHIAGISLGVLLLAGTALATEPADVEKYVNARIEIGEMMS
                     NYFKGGESYGSGQRPSPEKMKEMSDDINAKLSDLLSKRGLSLEEYRVRSKEIFANKEA
                     VDGYLAKHPDLKTRYDALPLSRMSGGGGSTGRGY"
     gene            complement(1080..1517)
                     /locus_tag="PK614_09010"
     CDS             complement(1080..1517)
                     /locus_tag="PK614_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82382.1"
                     /translation="MKRFATGLTTLAFAAALAAPALTYAAGPDKAPLEIKVRVSEKGF
                     FDDKGKPYGGKRMLQIPKDTMVKITFVFGEDLTSLAAGDTHQIALRAEDGWKQETSPL
                     WMMNRESTVSFLAGEKGRTQYRAYCILDCIGMEHLTNLIIQVV"
     gene            complement(1807..>2358)
                     /locus_tag="PK614_09015"
     CDS             complement(1807..>2358)
                     /locus_tag="PK614_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB82383.1"
                     /translation="IQHSPPRFILFHKPKGAVTTHQDEKGRQTIFDVLPPELKSLHAV
                     GRLDQATSGLLLLTNDSTLSSFLTDPKQQVPRLYLVTVRGEVSDETGRAALDGVDDQG
                     ERLHCASVTIQKRSGRESHLAVTLTEGKNREIRRLFKALGHEVTRLRRIQYGPFALGD
                     LPPGGWSELPIEKAKKLLNEGFS"
BASE COUNT          474 a          657 c          723 g          504 t
ORIGIN      
        1 ggcgctggtg ctggtctcga cgaccgacgg ggccatgatt ctggacaagg acatccctac
       61 gatcggtgcc ccgcgggact atcacgaggt ttcgaagaac atgatcgtcg agagttttcc
      121 tcccggtacg cagaccaaga cctatcagcc cttgctcgat cgcattccca cttggaatgc
      181 cacggtcatc cgggaagcgc tgacctccat gtcgcaattc aacgtgcatg gtcagctgac
      241 gcgcatctcg gtgccgacct tgattatggt gggcgagaag gatgatgtgg cgacgccggc
      301 gattgcgaag ggcattcagg cgcaaatctc cggtgcgaag ctggtcgagt tcaataccgg
      361 ccatttcatg atggccgaag acccggatcg atttcgcacg gtgctcgaag attttttgaa
      421 agggttgccg aaataggtgg gcaggcttgt gcatgtccgt ctgccgatgc gcaagctttc
      481 gccggaatgg tatgatgccc gaagtcagtt cacattgttc tattcactca gaggggacac
      541 tatgaccatg catgtatcgc gtcacatcgc cggcatctca ttgggcgtgc tgctgttggc
      601 tggaaccgca ttggcgacgg agcctgctga tgtggagaag tatgtgaatg cccgcatcga
      661 gatcggcgaa atgatgtcga actatttcaa aggcggggag tcgtatggtt ccgggcaacg
      721 gccgtcgccg gagaaaatga aggaaatgag cgacgacatc aatgccaagc tcagtgacct
      781 cctgtcgaaa cgcggcctct cattggagga atatcgcgtc cgcagcaagg agatctttgc
      841 caacaaggaa gccgtggacg gatacctggc caaacatcct gacctgaaaa cccgctatga
      901 cgcgctgccg ctgagccgga tgagtggcgg tggaggcagc accggtcggg ggtactaaca
      961 ggcatgtgac tgtcgctcta cggcttgctc acacagagaa acgctcggcg tattcatcaa
     1021 tacgccgagc gtgttcatgt caccctgctt ctgccggacg ggctgtttga gcagcctggt
     1081 tagacgacct ggatgatcag gttggtcaag tgttccatgc cgatgcagtc gagaatgcag
     1141 taggcccgat actgggtccg gcctttttca ccagcgagaa aactcaccgt cgattcccgg
     1201 ttcatcatcc acagcggact ggtttcctgc ttccacccat cttcggcccg cagggcaatc
     1261 tggtgagtat ctcccgcggc caggctggtc aggtcttcgc cgaagacgaa ggtgatcttc
     1321 accatcgtgt ccttggggat ctgcaacatc cgctttccgc catagggctt gcccttgtca
     1381 tcgaagaatc ccttttcgct cacgcgcacc ttgatctcca gcggcgcctt gtcggggccg
     1441 gcggcatagg tcagcgccgg cgcagccaag gcggcggcaa aggccaaggt ggtgagtccg
     1501 gtggcgaatc gcttcatgat ctccttcatg ttccttgatc tcctgtatcc gaccggcttg
     1561 cggtacaagt ccggcccatc cctgctcggg agcgacggtg gtaatgagtt gtggaggacg
     1621 agagcggagt gggttgttca gtacgatgcg gactgtctgt tcccgaacgc ttcgcctgct
     1681 gtactgacct tagcacagca ccggtagttg gcaagttgtt tcaaggagcg agcagcgagt
     1741 ggacgtagga aaagatgcag ccggtgaaaa cccttgtcac cacagggaaa acggcgatgc
     1801 gccgtctcag gaaaaaccct cgttgagcaa cttcttcgcc ttctcgatcg ggagttcgga
     1861 ccagccgccc ggtggcagat cgccgagcgc gaaggggccg tattgaatgc ggcgcagtct
     1921 ggtcacctca tgcccgagcg ccttgaagag ccgccggatc tcacgattct tgccttcggt
     1981 caacgtgacg gccagatggg attcccggcc tgagcgtttc tgaatggtga cggaggcgca
     2041 gtgcaaccgc tcgccctgat cgtccacccc gtcaagcgct gcccggcctg tctcgtccga
     2101 gacctcacca cgcaccgtga ctagatagag gcgcggcacc tgctgtttcg gatcggtcag
     2161 gaagctggag agtgtcgagt cgttggtgag caggaggagc ccgctggtcg cctgatcgag
     2221 ccggcccacc gcgtgcagac ttttgagctc aggggggagc acatcgaaaa tcgtctgccg
     2281 gcccttctcg tcctgatgtg tcgtgactgc acccttgggt ttatgaaaga gaataaatcg
     2341 cgggggactg tgttggat
//
LOCUS       DAORQN010000227         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1319_length_2349_cov_7.782040, whole genome shotgun sequence.
ACCESSION   DAORQN010000227 DAORQN010000000
VERSION     DAORQN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_2349_cov_7.782040"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK614_09020"
     CDS             complement(<1..225)
                     /locus_tag="PK614_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82384.1"
                     /translation="MRVDCGVLRKAMGIVVLSGLAIGLFGFYGPVWSASDPAMAAGQD
                     QSTAPPESSGSPRAGAEEKGFGGHRMRKACA"
     gene            462..1331
                     /locus_tag="PK614_09025"
     CDS             462..1331
                     /locus_tag="PK614_09025"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRB82385.1"
                     /translation="MQQIAPSSVPTDIIHRVHHKFWTYLLFWSVVAGSVIAVPGYGLI
                     YGYSWLDWTMFAILYVITGLGITVGYHRLLTHRSFECPDWVKACLLVAGGWALQNSGA
                     KWTADHLRHHAHCDDPADPYNAKRGFWYSHCGWLLDPVPCNDERFGSRVMQDRVAMWQ
                     HRYYAVIVLVGLALPFVVGFLHNGWKGGIGGFMLAGVGRTFAVLNSTFCINSVCHLWG
                     EQPYGTADSSRNSWFVSLLTFGEGYHNYHHTYPSDYRNGPLWYNFDPSKWLIYILWKL
                     GLASSLRTASSGH"
     gene            1697..>1850
                     /locus_tag="PK614_09030"
     CDS             1697..>1850
                     /locus_tag="PK614_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRB82386.1"
                     /translation="MGTKLYVGGLPYAATESQLSNLFSAHGTVESARVITDKFTGQSR
                     GFGFVEM"
     assembly_gap    1851..1877
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1878..2023
                     /locus_tag="PK614_09035"
     CDS             <1878..2023
                     /locus_tag="PK614_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRB82387.1"
                     /translation="ITALNGSDMDGRQLTVNEAKPQEARSGGGGGGGRFGGGENRGGR
                     SRF"
     gene            complement(2104..>2349)
                     /locus_tag="PK614_09040"
     CDS             complement(2104..>2349)
                     /locus_tag="PK614_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRB82388.1"
                     /translation="ALRDHRISRDGTIIIKAQDSRSQERNRALALDRLQELIQSVAVL
                     RPPRRPTKPTKGSTKRRLDSKSKRARLKSLRGKPDEQ"
BASE COUNT          459 a          731 c          629 g          503 t
ORIGIN      
        1 ggcgcaggcc ttccgcatgc gatggccccc aaagcccttc tcctcagccc ctgctctagg
       61 actgccggaa gactcgggcg gagcagtgga ttggtcctgg ccggctgcca ttgctggatc
      121 ggatgccgac cagacgggtc catagaaccc aaagagcccg atcgccaatc cactcaagac
      181 cacaatcccc atcgcctttc ttagaacccc acaatcaacc cgcatacgtc cccctttcgg
      241 aaataaatga ccgcacgagg tccactctcc ttccacaccc acgatgactg cgtgcggagc
      301 ctgttaccga cacagtgact caacgcctcc gcaggaaaac ggttgacggc caccgccagc
      361 ctcaggcaag agcggccgta acaaatctgc ggtccatcga ccatacggca ccgaccagac
      421 agacacttgc gaaaacccgc acgcttgact attgtagcga catgcaacaa atcgccccga
      481 gttcggttcc aaccgacatt attcatcgtg ttcaccacaa gttctggacc tacctcctat
      541 tctggtcagt cgtcgcgggt tccgtgatcg cggtcccggg ctatgggctg atctatggat
      601 actcctggct ggattggacg atgttcgcca tcctctatgt gattaccggg ctggggatta
      661 ccgtgggcta ccaccggctg ctgacccatc gcagtttcga atgtcccgac tgggtgaagg
      721 cttgcctatt ggtggcggga ggctgggcgc tgcagaactc cggcgcaaaa tggacggctg
      781 atcatttgcg acaccatgcg cactgcgatg atccggccga tccctacaac gccaaacggg
      841 ggttctggta cagccactgc ggctggctgc tcgatccggt gccgtgcaac gatgaacgat
      901 tcggctcccg cgtcatgcag gaccgggtcg cgatgtggca acaccgctac tatgcggtga
      961 tcgtcctcgt cggcctggcg ctcccctttg tcgtaggctt cctgcataac ggctggaagg
     1021 gcggaatcgg cggcttcatg ctggccggcg tcggacgcac ctttgccgta ctgaactcca
     1081 ccttctgcat caactccgtc tgtcacctgt ggggcgagca gccctacggt acagccgatt
     1141 ccagtcgcaa ctcctggttt gtctcattgc tgaccttcgg cgaggggtac cacaactatc
     1201 accacaccta tccgagcgac tatcgcaacg ggccgctgtg gtacaatttt gacccgtcga
     1261 agtggttgat ctacatctta tggaagttag gactcgcatc ctcgctccgg acagcctctt
     1321 ccggccactg aaccgcaccc attgtgtgcc aggccgagga acgaccggat caccaccaca
     1381 gagatcaggc cggagacgcg tggcccctct ccgtgcagcg atcgaccgca tctgaccgat
     1441 acaaactatt ttttccctgt ccatgaatcg tgtagcaatc cccctattca tctgttagga
     1501 tgcagtgtga tctcatgacg ggccgcatga gaatcatggt tccggatggc tcatcaccga
     1561 tgttgtttgt taccgagaag ttgatcggaa gtcagaccca agtcgcctca atcatcgcgt
     1621 cttcggcccg ctcattcccc tcatatgtct gttctcgtgt cgccgacaat gtcactattc
     1681 aaaaggagag acccctatgg gtactaaatt gtatgtcggc ggactgccgt atgctgcaac
     1741 tgaatcgcaa ctgagcaact tgttttccgc tcacggcacc gttgaatcgg cgcgcgtgat
     1801 cacggacaag ttcaccggac aatcgcgcgg cttcggcttc gtggaaatgt nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnncca tcacggcgtt gaacggctcg gacatggatg gccggcagct
     1921 caccgtcaat gaagcgaagc cgcaagaagc tcgctccggt ggcggcggcg ggggtggacg
     1981 gttcggcggc ggtgaaaacc gtggcggacg cagccgcttc taagaagtga cgggattcgt
     2041 ctcgtctccg aaggggcgct tcaactgaag cgccccttct tttttgccct gcgtctggtg
     2101 agactactgc tcgtcgggct ttccccgcaa cgatttcaga cgcgcccgct tgctcttgct
     2161 gtccagcctt cttttggtcg atcccttcgt cggctttgtc ggacgacgcg gcggacgcag
     2221 caccgccact gactgaatca attcctgcag acgatccaac gccaacgcac ggttgcgctc
     2281 ctggctccgt gagtcctgcg ccttaataat aatggtgccg tcacgcgaga ttctatggtc
     2341 ccgtaaggc
//
LOCUS       DAORQN010000228         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1322_length_2345_cov_8.161135, whole genome shotgun sequence.
ACCESSION   DAORQN010000228 DAORQN010000000
VERSION     DAORQN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_2345_cov_8.161135"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PK614_09045"
     CDS             <1..668
                     /locus_tag="PK614_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82389.1"
                     /translation="RILIQDHGQAGLVLALASNLLCPSPITGRKTLFDLLLAANPQGP
                     DAQAIQRIDPLLTNGTLLNGVALDIVTFGTPVRYGWDPSGIGKLLHVVNHRNLRTDGK
                     TWLSKMDLPQITVEMPIAWGGDYVQELAVAGSDALPAENAKATNKAVWELLEPYDGFE
                     RWVECARRAGRFPSEGQCLLVDYKDCTSSTNVRDHYYGHAAYTRTNAMLFNFTQIVSR
                     LYP"
     gene            832..1035
                     /locus_tag="PK614_09050"
     CDS             832..1035
                     /locus_tag="PK614_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82390.1"
                     /translation="MSPSPSHPDDVRIADNLRRTVELTELGLALRQAVIEQRNVPGAG
                     EGIAQVMHEIRLAKEQAWQQSQS"
     gene            1014..1550
                     /locus_tag="PK614_09055"
     CDS             1014..1550
                     /locus_tag="PK614_09055"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRB82391.1"
                     /translation="MAAEPILVRAFSDLISDFNRRGVNYALAGGWAYSALVEPRATTD
                     IDLLILLDPPSREQIQALVSSLFDSTIIHPSPMVFQGISIWRIVGIRSGQEVVIDFLL
                     ADSAYLRTALERRHTVPFGALQVPMLSLEDLIILKMVAGRLQDRADLEKIRAREADLR
                     VDWTYVERWKTMLGLADR"
     gene            complement(1676..>2345)
                     /locus_tag="PK614_09060"
     CDS             complement(1676..>2345)
                     /locus_tag="PK614_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82392.1"
                     /translation="WVLNDQDYDIGKELLAGQVSTAIPGSDWTCAVCGECHATEFVTC
                     WKCGVEKGVEGSQKVAIQSEASPSGGSLGFGVFLGVCLGVAATLCMYWLWRYSSMTST
                     AYDRNADGKDDVVYEYKGQFPWAAKLDNDFDGYFETALFYNQAGEVIRTEIDRGRAGR
                     PDLIEYNALGRLDSVEFIDRTTGRVKKRMYYKLDAKVREEIDEDGDGKFERVIEFNEF
                     ENPK"
BASE COUNT          515 a          726 c          591 g          513 t
ORIGIN      
        1 accgtatcct gattcaagac catggccaag ccggactcgt ccttgcgctg gcctccaatc
       61 tcctctgccc ttcgccgatc acgggacgga agacactctt cgatcttctc ctcgccgcga
      121 atccacaagg tccggatgcg caggccattc agcgcatcga cccgttattg accaatggca
      181 ccttactcaa tggcgtggcc ctcgacatcg tcaccttcgg cacaccggtg cggtacggct
      241 gggatccgtc cggcatcggc aaactgctcc acgtcgtgaa ccatcgcaat ctgcgcaccg
      301 acggaaaaac ctggctctcc aaaatggacc tgccgcagat caccgttgaa atgccgatcg
      361 cctggggcgg cgactacgtg caggaactgg ccgtggccgg tagcgatgcc ctgcccgctg
      421 agaatgccaa agccaccaat aaagccgtgt gggaattgct ggaaccctac gatggattcg
      481 aacgctgggt ggaatgcgcc agacgcgccg gacggttccc gagcgaaggc cagtgcctgc
      541 tggtcgatta caaagactgc acgagttcaa ccaatgtccg cgaccattac tacggccacg
      601 ccgcctacac ccgcaccaac gcgatgctct tcaacttcac tcagatcgtc agtcggctgt
      661 acccataagc ctgaccgctg acctcgcctg tccgtggtga cttcacatcg atgcctggtg
      721 ccaccctttc tatgagagag aaccgcggca gagcgcgcct tcctgcttgc tccccaccaa
      781 gtcgtcaagt agactctcct tcagagtccg tttttgctcg gagccccgat tatgtcacca
      841 tcgccctccc atccagacga cgtacgtatt gccgataatt tgcgtcgcac ggtcgaactg
      901 acggagttgg gcctggcctt gcgtcaggcc gtgatcgagc agcggaatgt gccgggcgcg
      961 ggtgagggaa tcgcacaggt catgcacgaa attcgactgg ccaaagagca ggcatggcag
     1021 cagagccaat cctagtccga gcgttctccg accttatttc cgacttcaat cgacgcggtg
     1081 tgaactatgc gctggccggg ggttgggcct acagcgcttt ggtggagcct cgagccacca
     1141 cggacatcga cctccttatt ctgctggatc cgccttcacg cgaacagata caggcgctcg
     1201 tctcatccct cttcgactcc accatcatcc atccgagccc tatggtcttt cagggcatct
     1261 cgatctggcg aattgtggga atccggtccg gacaggaagt cgtgatcgat ttcctcctgg
     1321 cagactcggc ctatcttcgc accgcattgg agcgcagaca cacggttccc ttcggcgcgc
     1381 tgcaggttcc gatgctgtcg ctcgaagatc tgatcatcct caagatggtg gcaggacggc
     1441 tgcaagatcg tgctgatctg gagaaaatcc gtgctcgcga ggccgactta cgcgtcgatt
     1501 ggacgtatgt tgaacggtgg aaaacgatgc tcggacttgc tgaccgttag gtggggcagg
     1561 attttaagaa gtatggtgga attattcgat cccggacgtt tcacgagcga aggccagtgc
     1621 ctgctggtgg attacaaaga agaattgccg gagacggagt aacggcgatt gcttatcact
     1681 tgggattttc gaactcatta aactcaataa cccgctcaaa cttgccgtcg ccatcttcat
     1741 cgatctcctc acgcacttta gcatctaact tgtaatacat gcgctttttg actctgcctg
     1801 tggttcggtc aataaattca actgagtcaa gtcttcccag tgcattgtat tcaatcaggt
     1861 ccggccgtcc ggcccgaccg cgatcgattt ccgtacgaat gacctcacca gcttggttgt
     1921 agaaaagcgc ggtctcgaaa tacccatcaa aatcattatc caacttggca gcccatggaa
     1981 attgcccctt atactcatag acaacatcgt ccttgccgtc cgcgttccga tcataggctg
     2041 tgcttgtcat tgaagaatat ctccacagcc aatacatgca cagtgtggcg gcaactccga
     2101 gacagacccc gaggaaaaca ccaaatccga gcgagcctcc tgaaggcgac gcctctgact
     2161 gaatcgcaac cttctgagat ccttccaccc ccttctccac accacacttc cagcaagtga
     2221 caaactccgt cgcatgacac tcgccgcata ctgcacatgt ccaatcagac cccggtatgg
     2281 ccgttgaaac ctgcccagca agcaactctt ttccgatgtc ataatcctga tcgttgagca
     2341 cccag
//
LOCUS       DAORQN010000229         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1337_length_2315_cov_4.857522, whole genome shotgun sequence.
ACCESSION   DAORQN010000229 DAORQN010000000
VERSION     DAORQN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1337_length_2315_cov_4.857522"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PK614_09065"
     CDS             <1..545
                     /locus_tag="PK614_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82393.1"
                     /translation="IIRKDKKVVWQVMGDMYMEMPLDQSNAPDMGGFDIQEQTEVGEE
                     TVNGLKTTKTKIIATKPDGSKFGGFFWTTKEGVTVKMDLLSKDGDKKMRMASELTNVK
                     IEKQNPALFEVPAGATKNDMGAMMGQGGMPNMEEMMKNAGHEREKEKRSSRSNSGNKS
                     QESETPAGEVNKMLKGLFGR"
     gene            558..1535
                     /locus_tag="PK614_09070"
     CDS             558..1535
                     /locus_tag="PK614_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82394.1"
                     /translation="MRTVRILSCGLLLLAVMVSPVRADDPCLGDEEEKSAKAAFAALT
                     KAEQAGRQAELFMAYRSILGNECLDRYNKTALSRAKTGVPKLGRDLAKAAEAKGLLYS
                     ADPVRSDGQTSAFRYFETIGDYADANRVMMKALQAKPDDLALFTTAWGVDEGRWVVPD
                     PKTGERQPYVSPQAYRQDLQKMASAKADRLMKAEEHDAKGLSGSAVEVATSAMKSLEK
                     LSAAAAWMKFLQAGDKAARERAEQRGDTITARSDSTFTQANAVMYYEFAGSPKAKDKL
                     VQVKKKMEESSRALEKSGEKVKGAFAEQSQAEQKKFDKKKADLEKELGF"
     assembly_gap    2090..2107
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2108..>2315
                     /locus_tag="PK614_09075"
     CDS             <2108..>2315
                     /locus_tag="PK614_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82395.1"
                     /translation="LNENRAERHGHALPKAGDEVTVVIDENNMVLEVHPKGTEGKHRF
                     VTGEVVSIGTMQNEITLKTAKGGAE"
BASE COUNT          555 a          571 c          740 g          431 t
ORIGIN      
        1 ctattatccg caaggacaag aaggtcgtct ggcaggtgat gggcgatatg tatatggaga
       61 tgccgctgga tcaatcgaac gcgcccgaca tgggcggctt cgacattcag gaacagaccg
      121 aggtcggtga agagaccgtg aacggcttga agaccacgaa aacgaaaatc atcgcaacca
      181 agccggacgg gtcgaagttc ggtggcttct tctggaccac caaagagggt gtcacggtga
      241 aaatggatct cctctcgaaa gatggcgaca agaagatgcg catggccagc gagttgacga
      301 atgtgaaaat cgagaagcag aaccctgcgc tcttcgaggt gcccgccggc gcgacgaaga
      361 acgatatggg tgcgatgatg gggcagggcg gcatgccgaa catggaagag atgatgaaga
      421 acgctggaca tgaacgagag aaggaaaaac gatcgtccag gagcaacagt gggaacaaga
      481 gccaggagag cgagacgccg gcgggcgagg taaacaagat gctcaaaggc ctcttcggtc
      541 gataggggag gtgcgcgatg cgtacggtca gaatcctgag ctgtggcctg ctgctgcttg
      601 ctgtgatggt gtccccggtc cgcgccgacg atccctgttt gggggatgaa gaagagaaga
      661 gcgccaaggc ggcgttcgct gctctgacga aggccgaaca ggccggtcgg caggccgagt
      721 tgttcatggc ctatcgatcc atcctcggca acgagtgtct cgatcgttac aacaagaccg
      781 ccttgtcccg cgccaaaacc ggtgtgccga aactgggccg ggatttggcc aaggcggctg
      841 aggcgaaggg ccttttgtat tctgccgatc cggtgcggag cgatgggcag acctcggcgt
      901 ttcgctattt cgagaccatc ggggattatg ccgatgcgaa tcgtgtgatg atgaaagcgc
      961 tgcaggcgaa accggacgat ctcgcgctct tcacgacggc ctggggtgtg gatgaaggtc
     1021 gttgggtggt gccggatccg aaaacaggag agcgtcagcc ctatgtctcg ccacaggcct
     1081 atcgtcagga cttgcagaag atggcatccg cgaaggccga tcggctgatg aaagcggaag
     1141 agcacgatgc gaagggactt tcgggcagcg cggttgaggt ggccacgtcc gcaatgaaat
     1201 ctctcgaaaa actgagtgcc gccgcggcgt ggatgaagtt cttgcaagca ggcgacaagg
     1261 ctgcccggga gcgggccgag cagcggggcg atacgatcac ggcgcgttcc gattcgacgt
     1321 ttacccaggc gaatgccgtg atgtattacg agttcgccgg ctcgccaaag gcgaaagaca
     1381 aactggtgca ggtgaagaag aagatggaag agtccagccg tgcgttggag aagtccggtg
     1441 agaaagtgaa gggggccttc gcggagcaga gtcaggcgga gcagaagaag ttcgacaaga
     1501 agaaagcgga tctcgaaaag gaactcgggt tctagccatg ggccccggct tccctcgtcg
     1561 ttcattgtcg cgtgaacgga gggtacacct cggctattcc ctctcggtac gatggaaatc
     1621 atgccgttcg tttcaaggat cgcatatttg atttgttcca tccgttccca accatgcttt
     1681 tccggcgcgg ccgtgaggat atcctgttca tccgcgcgcc cccggtgcat gaactccttc
     1741 agcggccggc cttgatcgac caggatcaac ggtagtccat tcagataccg ctccacgcag
     1801 gaacccgcac tcatcaggct cttcttctct ctgaacttac gccagggctt ctcctagtcc
     1861 ttcactacgc agaaagccgc tattctccac tctactgagt acgtcgtagg gagatcttac
     1921 agacgactcg ctaacgggag ggtaggatat gtcgaaattg ttcacatatg tgtgcgtggt
     1981 attggctgtg gttggaatga cgagtcaggt tggaagggcc gacgaaggct cccacaaagg
     2041 ctcccccaat agtcaccacc aggagctgca aggggtggtg gtggagaagn nnnnnnnnnn
     2101 nnnnnnnctc aatgagaacc gtgccgagcg tcatggtcat gcattgccga aagccggcga
     2161 cgaagtcacc gtggtcattg atgaaaacaa catggtgctg gaagtgcatc ccaagggcac
     2221 ggaaggcaaa catcggtttg tcaccggtga agtcgtgtca atcggcacaa tgcaaaatga
     2281 gattacatta aagactgcga aggggggagc agaag
//
LOCUS       DAORQN010000230         2310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1340_length_2310_cov_174.881153, whole genome shotgun
            sequence.
ACCESSION   DAORQN010000230 DAORQN010000000
VERSION     DAORQN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_2310_cov_174.881153"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="PK614_09080"
     CDS             <1..179
                     /locus_tag="PK614_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB82396.1"
                     /translation="CLNEHWFLTLAHAQAIIEAWRREYNEERPKKALGGLTPAAYARH
                     LAQKSGTVASGLQT"
     gene            complement(585..2081)
                     /locus_tag="PK614_09085"
     CDS             complement(585..2081)
                     /locus_tag="PK614_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82397.1"
                     /translation="MKLSLSFCIFDESKAYYLISAVLLLLQLCPIWSTPYPAMHDYPN
                     HLARVHVLHEYGENKSYQALYERDTRPIPNLAIDLIVSTFMSIASIETSSKMFLSLMV
                     VVFNLGIHLFGFAVNRGPYWSVLVCTFFTYNFAFSYGFVNYLFGLGVFFGTLAIWLHF
                     RPQWTLARIVLTSLFTLVCYVSHLSAFVFFSLSIVCLTGIQLIKNRLLHIEHVLGLTV
                     LVPPTLLYFLYSHGIDRTAPMIWWRPLLVKKVTGLVYPFLTYDLFIDLGLGLGFVVFV
                     LFLLRGKGGTTINWESILLSGVFVALYLCAPMSGAQSSYVDRRFLLPATLFLLLGLKI
                     NVLNKTGRHILIGLLCLTILRLGEVVYYWNRIGVEVQAQVHMLDKLPTGVRLYPMVLH
                     ESSARNWLWDMHFFFTAHYATIYRNAFVPTIYAWKGAHPLNLRTGNNDYFQVEQGTPV
                     ANVNWEVIFSNYDYLWGYKLPQEFRRFLVTKGELIAQSDEAMLVRIRK"
BASE COUNT          663 a          561 c          577 g          509 t
ORIGIN      
        1 agtgcctgaa cgagcattgg ttcctcaccc tcgcgcatgc ccaagccatc attgaagcgt
       61 ggcggcgcga atataacgag gagcgaccga aaaaagcatt gggcggactg acacccgccg
      121 cctatgcgag gcatttggct caaaaatcgg gtacagtcgc atccggactc caaacgtagc
      181 tgctactcaa gacgggggga cgtcggatcg acacgatttg ggtctcggtg aacttcgact
      241 gacgcatggg aacctcctgg ctagggtggc tattctgcca gaatgttctc cctgtgactg
      301 tctcgatcta tggggagctt acggcttgat ttcaagggag cactacaaaa tcagcgcgga
      361 atggctacag agttacagtg aggagcggcc tcatgagtcg ttggccaacc tgccgacggc
      421 catatatcgc gtccacttga aaaccccgca gctttccgtt cgcactgtga ccttgacaat
      481 gaggcttaca agacctcacc tcttgttaag acttacggcg acaaggtacc aaaggtatag
      541 gatgaaagtc cctgaacggc catcccatat tgacaggagt cccttcactt gcgaatacgg
      601 accaacattg cctcgtcgga ttgggctatt aattctccct tcgtaacaag aaaccgcctg
      661 aattcctgtg gtaacttata tccccacaaa tagtcgtagt ttgagaagat cacttcccaa
      721 tttacgttgg caacgggtgt accctgttcg acttggaagt aatcgttatt gcctgtccgc
      781 aagttcagcg ggtgcgcccc cttccaggca taaattgtgg gaacgaatgc atttcgataa
      841 atagtagcgt agtgggccgt aaagaagaaa tgcatgtccc ataaccaatt tcgagctgaa
      901 gattcgtgca agaccattgg atacagccga accccggtgg gcaacttatc aagcatgtga
      961 acttgagcct gtacctctac tccaatccga ttccaataat atacgacttc ccctagccgt
     1021 aagatggtta ggcacagaag gccaatcaag atatgtcgtc cagttttgtt caggacattg
     1081 atcttgagtc ccagaagcag aaataaggta gcgggtaaga gaaatcggcg atctacgtag
     1141 ctggattgtg cgccgctcat tggggcgcac agatagagag ccacaaacac ccctgaaagc
     1201 agaatcgact cccaattgat tgtcgttccc cccttccctc tcagtagaaa cagcacaaac
     1261 acaacaaacc caagaccaag accgaggtct atgaaaagat cataagtaag gaacgggtac
     1321 actagtccag ttaccttctt cacgagaagt ggacgccacc atatcatggg tgctgtccta
     1381 tcaattccat ggctgtacaa gaaatacagc agcgtgggag gaacaagcac tgttagtcct
     1441 agcacatgtt cgatatggag caatctattt tttatcaatt gaattccagt caagcacacg
     1501 attgaaaggc taaaaaatac aaatgcggag aggtgggata catagcagac cagggtaaag
     1561 agcgaggtca agacgattcg agccaatgtc cactgcggcc ggaaatgcaa ccagatagcc
     1621 agtgtgccga aaaacactcc aagtccaaat aggtagttca cgaatccata agaaaaagcg
     1681 aaattgtacg taaagaatgt acagacgagc acactccaat aagggcctcg attaacagcg
     1741 aacccgaaaa gatgaatgcc taggttaaac acgactacca tcagtgaaag aaacattttg
     1801 ctagacgtct ctatgcttgc gatgctcatg aacgtcgaca caatgagatc aatggccaag
     1861 tttggaatcg gcctagtgtc acgctcatac agagcctgat acgatttgtt ttcaccgtac
     1921 tcgtggagga cgtggacccg cgccaagtgg ttgggatagt catgcatagc gggatatggg
     1981 gtcgaccaga tcggacagag ctgaagcaga agaagaactg cagagatcag atagtaggct
     2041 ttcgattcat caaatatgca aaaagagaga gagagtttca ttcttacacc caacgacggt
     2101 ggtcccgtta acaagagtag gcgagctttt tggcgagcta gctcacctga tgatgcatgt
     2161 aaactcatcc ggcgtcaggt caccgagtga gggcgctgct gaatgtatgc cctccgccag
     2221 gcctcgaaaa tggtggggcc tcagttaatg aggcgaactg ttggaggttt aagcactcgt
     2281 cgcgcagtca tctattaaac gactcaatga
//
LOCUS       DAORQN010000231         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1367_length_2252_cov_5.143832, whole genome shotgun sequence.
ACCESSION   DAORQN010000231 DAORQN010000000
VERSION     DAORQN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_2252_cov_5.143832"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PK614_09090"
     CDS             <1..813
                     /locus_tag="PK614_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB82398.1"
                     /translation="LRDKYQCRHFLFSDESYPPALFKKVSQLLVDRNVGIKWTTLIRF
                     EETLQDQAVWDLAAKAGCCTLYYGMESANERVLNLMDKHAKKNVIQNNLHQAAKAGIW
                     NHVMAFYGFPGETREEALETRQFVIENQPVIHSVELFYFVAYRHTPMVRNPDKFGITI
                     HKQEEYDLPLDYYYTLNEPVGISCLDAMQLCEEFYKNDFSPWAVRVNSREHVFLYISK
                     FGTNKLPQIYAATKDGATTAEGVSGLVTWPMAIGEGDEGKEGMSRVVSHGVG"
     gene            complement(1007..1276)
                     /locus_tag="PK614_09095"
     CDS             complement(1007..1276)
                     /locus_tag="PK614_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82399.1"
                     /translation="MTEELLTKALALFTLTQPYTREQLNQKREELLHTWTPRRFANLT
                     NNPKKYMQAYTKAEEMTRQIESSYALLATALGRDEARNPDVRRKT"
     gene            1359..>2252
                     /locus_tag="PK614_09100"
     CDS             1359..>2252
                     /locus_tag="PK614_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82400.1"
                     /translation="MATALPIFQPTPIFKDRTDYRQVLIKEMEAGKIPLSLGRDCPVK
                     CEFCYELDHSYRETLDPPKTSDEDWKFILNYISKKPTDPTQFWCLGGNEFMEWTDLFL
                     HPKAMEWVEDFLRETDKSIQFFTVGFVHVPKIHQLVAQYPGRINFELSVITLSDYRQR
                     LMPHAPSVKHVMKVLDGPAVSSANFYAFDKDTMSKDAATISKVNRQCVLWMGTLTPVR
                     GLKEETAALMRQGRTFLPEEASHVYDLALPNLQTIHTEAYTTAFLSRRRIVSLFDSLE
                     LEKKDTVVMAGSVHKILTMYRK"
BASE COUNT          475 a          627 c          681 g          469 t
ORIGIN      
        1 ctacgggata agtatcaatg tcggcacttc ctgttctccg acgaatccta tccgcccgcg
       61 ctgttcaaga aagtgtcgca gctgttggtg gaccggaacg tcggcatcaa gtggacgacg
      121 ctgatccggt tcgaagagac gctccaggac caggcggtgt gggacctcgc ggcgaaggcc
      181 ggctgttgca cgctctatta cggcatggag tcggcgaacg agcgcgtgct gaacctcatg
      241 gacaaacacg cgaagaagaa cgtgattcag aacaatctcc accaggcggc gaaggccggc
      301 atctggaacc acgtgatggc gttttacggc ttccccggcg agacgcggga ggaagcgctg
      361 gagacgcggc agttcgtcat cgagaaccag ccggtgattc attccgtgga gctgttctac
      421 ttcgtggcct accgccacac gccgatggtg cgcaacccgg ataaattcgg catcacgatc
      481 cacaagcagg aggagtatga cctgccgctg gattactact acacgctgaa cgagccggtg
      541 ggtatttcct gcctggatgc gatgcagctc tgcgaagagt tctataagaa cgacttcagt
      601 ccctgggccg tgcgggtgaa cagccgcgag cacgtctttc tctatatctc caagttcggg
      661 acgaacaagt tgccgcagat ttacgcggcg acgaaggacg gcgcgacgac ggcagaaggt
      721 gtgtctggtc tcgtgacctg gccgatggcg atcggtgaag gggatgaggg gaaagagggg
      781 atgagtcgcg tcgtctctca cggcgtcggc tagcaggcag gctggtgaaa acgccatctc
      841 caggcgttct cggtcgcctg tctccctgcg acgtaccgaa agggtacgcc tcagtcgcca
      901 cgctccctgc gtctttggca tatggcgttt tgaccagcct gcgagatgcg gattgtaccg
      961 gatgctgaaa acgacctccc acgtcgttct cggctcgttt cactgttcac gttttacgtc
     1021 tcacgtcagg attccgcgcc tcgtcccggc cgagggccgt tgcgagcaaa gcatacgacg
     1081 attcgatttg ccgggtcatc tcttcagctt ttgtgtaggc ctgcatgtat ttcttaggat
     1141 tgttggtcag gttggcaaag cggcgcggcg tccaggtgtg taggagttcc tcgcgcttct
     1201 gattcagttg ttctcgggta tagggttgcg tgagggtaaa gagtgccagg gctttggtga
     1261 ggagttcctc ggtcatgtca cggagtctgg caagtcgata atgacgctgt caatctagca
     1321 ggcgttgccc ctcgcggcaa caggcaaggt gttccaccat ggccactgca ctcccgattt
     1381 ttcagcccac cccgatcttc aaggatcgca cggactatcg acaggtgctc atcaaagaaa
     1441 tggaggcggg gaaaattccc ctaagcctgg gccgggactg ccccgtcaaa tgcgagttct
     1501 gctacgaact cgaccactcc taccgcgaaa cgcttgatcc gccgaaaacc agcgacgaag
     1561 actggaagtt catcctcaac tacatcagca agaaacccac cgatcccaca caattctggt
     1621 gcctgggcgg caacgagttc atggaatgga ccgacctgtt cctgcatccc aaggcgatgg
     1681 aatgggtgga ggattttctc cgcgagacgg acaagagcat tcagtttttc acggtcgggt
     1741 tcgtccatgt gccgaagatc catcagctgg tggcgcagta tcccggccgc atcaacttcg
     1801 aactctcagt catcacgctc agcgactatc ggcagcgatt gatgccgcat gcgccttccg
     1861 tgaaacacgt catgaaggta ttggatggcc cggccgtctc ctcagcgaat ttttacgcgt
     1921 tcgacaagga cacgatgtcg aaggatgcgg cgacgatctc caaggtgaat cggcagtgtg
     1981 tcctgtggat ggggacgctg actccggtgc gcgggctcaa ggaggagacg gcggcgctga
     2041 tgcggcaggg gagaacattc ctgccggagg aagccagcca cgtctatgat ctggcgctgc
     2101 ccaatctcca aacgatccac acggaggcgt acaccaccgc cttcttgagt cgccggcgta
     2161 tcgtcagcct gttcgactcg ctggagttgg agaagaaaga cacggtcgtg atggcgggca
     2221 gcgtccacaa aatcctgact atgtaccgga ag
//
LOCUS       DAORQN010000232         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1370_length_2250_cov_2.497039, whole genome shotgun sequence.
ACCESSION   DAORQN010000232 DAORQN010000000
VERSION     DAORQN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_2250_cov_2.497039"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK614_09105"
     CDS             complement(<1..539)
                     /locus_tag="PK614_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRB82401.1"
                     /translation="MRINKFFTEQGLCSRREADRLIAEGRVTINGQVAKLGDQVSPQD
                     LVARDGVVLQRGNRSVYLKYHKPVGVTTTTEPHVRRNIIAEIGHTARIFPIGRLDKDS
                     SGLILLTNDGDIVNEILRVEHGHEREYRVEVDREFDETFLARMAQGVTILGVPTRPCI
                     VARLGPRRFRIILKEGRNR"
     gene            complement(730..>1535)
                     /locus_tag="PK614_09110"
     CDS             complement(730..>1535)
                     /locus_tag="PK614_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRB82402.1"
                     /translation="SSYILGSDRGPSAIIEASQQVELYDETLRYEPYRQWGGVATAAT
                     LNLDGRVDGQAVQAIQDFVQPHVGRGKFLVTLTGEHTGALGAIRAHARNYPGMCVVQI
                     DAHGDLQDNPYSHASVMARVVEDGLPLVQIGVRSISPEEIDLIQKTPRIHTFFAASIL
                     DPSGPYEGRAARWIPEVLAACTGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDPLAL
                     VTALANGPGIVGMDISEIAPIEGFVAPQFSIARLIYRMLGRIRAGRRVH"
     assembly_gap    1536..1545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1670..>2250)
                     /locus_tag="PK614_09115"
     CDS             complement(1670..>2250)
                     /locus_tag="PK614_09115"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HRB82403.1"
                     /translation="YMVPTHMFLTRGVGVHKEKLAAFEQALRSAGVAYCNLVSVSSIL
                     PPNCKILPRKRGEKLLNPGEITFCVMARSETNERNRLISASIGLAIPTDRDTYGYLSE
                     HHAYGETDEESGEYTEDLAAQMLATTQGIEFDPDVAWKEREQVFKMGGKIVRTLNITQ
                     SAVGRPNRWTCVIALAVFIPTENIPKSLRNKA"
BASE COUNT          463 a          691 c          652 g          434 t
ORIGIN      
        1 tgccgattgc gcccttcctt caggatgatg cggaagcgac gggggccaag ccgcgccacg
       61 atgcaaggcc tggtgggcac ccccagaatg gtcacgccct gggccatccg cgcgagaaac
      121 gtctcgtcga attcccgatc cacttccaca cgatattccc gctcatgtcc gtgctcgacc
      181 cgcaagatct cattgacgat gtcgccgtcg ttcgtgagca gaatgagtcc ggaagaatct
      241 ttgtcgagcc gtccgatcgg gaaaattctg gccgtatggc cgatttcggc gatgatgttc
      301 cgccgcacat gcggttcagt ggtcgtcgtc accccgaccg gtttgtgata cttgagatac
      361 accgagcgat tgccgcgctg cagcacaacg ccgtcgcgcg cgaccaaatc ctgtggcgag
      421 acctggtcgc cgagcttggc cacctggcca ttgatggtca cccgcccttc ggcaatgagg
      481 cgatcggcct ctcgccgtga acacaggccc tgctcggtga aaaatttatt gatgcgcatg
      541 gcggggaatg aactcgcgtg agccgtccct catgaagcgt ccgtcgtatc tcgcaaagag
      601 cgtatggaca aaagcctcga ccgaaaaaca caagcagagc tctctcattg cctgtgttct
      661 aagtcatgag cgctccattc cgctctccaa cgagagacgc tgcaggagcg acgtttcaca
      721 atgtgagagt caatgcacgc ggcggccggc gcgaatccga cccagcatcc gatagatcaa
      781 gcgggcgatg ctgaactggg gcgcgacaaa cccttcgatg ggcgcgatct cgctgatgtc
      841 catgccgacg atgccgggac cgtttgccaa cgcagtcacc agcgcgaggg ggtcgtacca
      901 acccaacccg cccggctcag gcgttcccaa tgccggcacg agcgacgcat cgagcccgtc
      961 gcaatcgaag gtgaggtaca ccggcccggt gcaggcggcc agaacctcgg gaatccaacg
     1021 agcggcccgc ccctcatagg gaccggatgg atcgaggatc gaggcggcaa aaaatgtatg
     1081 gatccgtggg gtcttctgaa tgagatcgat ttcctccgga ctgatcgatc gaacaccgat
     1141 ttgtaccagg ggcagcccat cctccaccac acgggccatc acactcgcat ggctgtatgg
     1201 attgtcctgg agatcgccat gggcgtcgat ctgcaccaca cacatacccg gataattccg
     1261 ggcatgcgcc cgaatcgccc ctaaggcgcc ggtatgttca ccggtcaggg tgaccaaaaa
     1321 tttcccccga ccgacgtgcg gctgcacaaa atcctgaata gcctggacgg cctggccatc
     1381 gacccgccca tcgagattta atgtggctgc cgtcgccacg ccgccccatt ggcggtaggg
     1441 ctcgtagcgc agcgtctcat cgtataactc gacctgctgg gaggcttcaa tgatcgcgga
     1501 ggggccccga tcggacccga ggatataact ggaggnnnnn nnnnntgttc gtagggagcc
     1561 gggagtacgt agactccggc ctgatcggga tgacaccagg gctcgtcgat ccccagaaag
     1621 ttgtcggctt gcccaagcca tccggacggg agcgccatgc cgcccgccgc taggccttgt
     1681 tccgaagact cttggggatg ttttccgtgg ggataaacac cgccaaggcg atgacgcacg
     1741 tccaccgatt gggacgaccg accgccgatt gggtgatatt caaggtccgc acgattttgc
     1801 cgcccatttt gaagacctgc tcacgctctt tccaggccac atcgggatcg aactcgatgc
     1861 cctgcgtggt cgcaagcatc tgtgccgcca agtcttccgt atattcaccg gactcttcgt
     1921 ccgtttcacc ataggcatgg tgctcggaga gatagccgta cgtatcgcga tccgttggaa
     1981 tcgccaagcc aatcgacgcg gagatcagcc ggttgcgctc gttggtctcc gatctggcca
     2041 tgacgcaaaa cgtgatttca ccggggttca acaatttctc gccgcgctta cgagggagaa
     2101 ttttgcagtt gggggggaga atcgaagaga cgctgacgag gttgcagtat gccacgccgg
     2161 cgctccgcaa agcctgctcg aacgcagcca gcttttcctt gtgcaccccg acacccctcg
     2221 tcaaaaacat gtgtgttggt accatgtagg
//
LOCUS       DAORQN010000233         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1381_length_2222_cov_22.504384, whole genome shotgun sequence.
ACCESSION   DAORQN010000233 DAORQN010000000
VERSION     DAORQN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_2222_cov_22.504384"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..863
                     /gene="dat"
                     /locus_tag="PK614_09120"
     CDS             24..863
                     /gene="dat"
                     /locus_tag="PK614_09120"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HRB82404.1"
                     /translation="MPDIAYVNGRFGPLADAVVSIEDRGFQFGDGVYEVIRTYRGQPF
                     AIEEHLARLERSAQALQLSIGQTRAQWSSLIREGLRLSQFPEAKIYLQITRGQAPRDH
                     PFPVSSAPTTVLTFRELHPLDVSVRNAGVQAMSVEDIRWGRCDIKSVNLLANVLARQR
                     AKEAGVFEAILVREGTVTEGSVSNVMVVRNGAVQTAPEGRRILSGVTRAIVLDLARKE
                     GIPVAESFVTREELLAASEVFLTGTTVEVLPVVRIDGHAIGGGSPGPVSQLLSRRFEA
                     LVG"
     gene            953..1471
                     /gene="rimP"
                     /locus_tag="PK614_09125"
     CDS             953..1471
                     /gene="rimP"
                     /locus_tag="PK614_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRB82405.1"
                     /translation="MSDAGAVSAGKPSVKRAEALNLRIQEIAAPILQSHGLELVEAVC
                     VGQGPRTVIRVFIDKPGGITLTDCEQAHRSLSPALDVIDPFPHAYTLEISSPGLDRPL
                     RSVQDYRRLIGKPVTLKLCEPIQAQWRLVGAVVEVDDRGVTLAIQQKKTTETIRVEFD
                     QIALARRKVEFS"
     gene            1514..>2222
                     /locus_tag="PK614_09130"
     CDS             1514..>2222
                     /locus_tag="PK614_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HRB82406.1"
                     /translation="MNRELIAVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVDTVANPKAEISLKEAREYDEGAEVGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNFLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKALLLEVRRTPKDVQVILSRSHPQFVAKMFELEVPEVGEK
                     IVEIKSIVREPGDRTKIA"
BASE COUNT          464 a          565 c          712 g          481 t
ORIGIN      
        1 cgaaaggtgc tgagaaatga gtcatgcctg acattgcgta tgtgaatggt cgctttggtc
       61 cgctggctga tgcggtcgtc agtattgagg atcgaggctt tcagttcggt gacggggtct
      121 acgaagtcat tcggacctat cgtggacagc ccttcgccat tgaggaacac ctggctcgat
      181 tggagcggag tgctcaggcg ctgcagcttt caatcgggca aacgagggct cagtggagtt
      241 ccttgattcg agaaggcctg cggctgagcc aattccctga agccaaaatt tatctccaga
      301 tcactcgcgg gcaggctccc cgcgatcatc cattccctgt atcgtccgcc cccaccaccg
      361 ttctgacctt tcgcgaactc cacccgctgg atgtctccgt tcgcaacgcc ggggttcagg
      421 ctatgtcggt cgaggacatt cgatggggac gctgtgacat caagagcgtc aatcttctgg
      481 ccaacgtcct ggcgcggcag cgagccaagg aggcgggcgt gtttgaagcg attctggtcc
      541 gagaggggac ggtcacggaa gggtcggtca gcaatgtcat ggtggtgcgg aacggcgccg
      601 ttcagacggc gccggaaggg cgcaggattc tgtccggggt gacgcgcgcg atcgttctgg
      661 atctggcgcg caaagaaggt attccggtgg cggaatcgtt cgtcactcgg gaagaattac
      721 tcgcggcgtc ggaggtattt ctgacaggaa cgaccgtgga ggtattgccc gtggtgcgta
      781 tcgacggaca tgcgattggg ggcggatcgc ccggccccgt ttctcagtta ttgtctcgcc
      841 gttttgaagc gctggtcggc tagccgcccc ttgctttcac tactatggca tggtaaggtt
      901 ctcgcgctgt aagtgggcca aggcccactt ttttgtttga gcaacttttt ctatgagtga
      961 cgcgggagca gtgtcggcag ggaagccgtc ggtgaaacgc gctgaggcgc tgaaccttcg
     1021 catccaagaa attgccgctc ctattttgca gtctcatggt ctggagttgg tcgaggccgt
     1081 ctgcgtcggc caagggccca ggaccgtgat tcgtgtgttt atcgacaagc ctggggggat
     1141 caccctgaca gactgtgagc aggcgcatcg ctcgttgagc ccggcgctcg atgtgattga
     1201 tccattcccg catgcctata cgctggaaat ttcgtctccc gggctggatc gtcccttgcg
     1261 gagcgtgcaa gactatcggc gtctgatcgg gaagccggtc acactcaaat tgtgcgaacc
     1321 tattcaggcg cagtggcggc tggttggcgc agtggtcgag gtggacgacc ggggggtgac
     1381 tcttgccatt cagcagaaga aaaccacgga gacaatccga gttgagtttg atcaaatcgc
     1441 tctggcgcgg cggaaggtgg agttctcgta gcagcaggtg ccagggtggt gtgttaacca
     1501 tgaggtgtga gctatgaacc gagagttgat cgccgtcatc gatgaaatcg gccgtcaaaa
     1561 agggatcgat aaggccaggg tcattggcgc gatcgagtca gcgcttcaaa ctgctgccaa
     1621 gaaacgattc ggccaggccg aaaacatcca ggtggaaatc gatccgaaga ccggcgaaat
     1681 ctcggtcgtt tcaaagaaaa tcattgtcga tacggtggcc aatccgaaag cggaaatctc
     1741 gctaaaggaa gcgcgcgaat atgacgaagg cgcggaggtt ggcgatgaga tcggctcatt
     1801 gatcgagatg gacgagttag gccggattgc ggcccaaacg gccaagcagg tgatctttca
     1861 gaaagtgcgc gaagccgagt gggaagctgt tcagaaggag tactcgaccc gtcagggtga
     1921 tttggtgaac ggcattattc tcggcatgga gcgccgaaac tttctggtcg atctcggcaa
     1981 gaccgaagcg attcttccca ttcaggaaca gatcccacgc gaaacctatc gtcgcggaga
     2041 ccgggtgaag gccctgctct tggaggtgcg tcgcaccccc aaggacgtgc aggtcattct
     2101 ttcccgcagc cacccgcagt ttgtggcgaa gatgtttgag ctcgaagtgc cggaagtcgg
     2161 cgaaaagatc gttgaaatca agtctatcgt gcgtgagccc ggcgatcgta ccaaaattgc
     2221 gg
//
LOCUS       DAORQN010000234         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1389_length_2203_cov_2.074488, whole genome shotgun sequence.
ACCESSION   DAORQN010000234 DAORQN010000000
VERSION     DAORQN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_2203_cov_2.074488"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..>746)
                     /locus_tag="PK614_09135"
     CDS             complement(328..>746)
                     /locus_tag="PK614_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82407.1"
                     /translation="FLVPASRLAATFAPQPASVRAYLTGFSEPALDAFAAVQPGARSV
                     YRCSVHCALGELPEVLATIEPQGRLYSTLCEFQTQELMPEGDDASAIIGIVGTAGGFQ
                     LEARLNRAPLPEEHMAAWLERLLGYDVTYAPLPPFI"
     assembly_gap    747..756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<757..1398)
                     /locus_tag="PK614_09140"
     CDS             complement(<757..1398)
                     /locus_tag="PK614_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82408.1"
                     /translation="MRERRWETSGRLTFREVLAVGERLATLGLKPAHPAKDVICYVED
                     WAGDSPDAFDQLDHWATEDVTLLHIREAWQGDFFLLAGGYHTVYQRFQDVGTYCSISH
                     PWRTKPGLRFHHPTCMFWLGFRHNHAFIRVRLHTQEVVTPGETREDARRVEWIDERRA
                     IFLDAIGTLDLPVETSVEKGGLVLRPADPATPFFCSWPDAFGPCQFEYNSLDAY"
     gene            complement(1444..2073)
                     /locus_tag="PK614_09145"
     CDS             complement(1444..2073)
                     /locus_tag="PK614_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82409.1"
                     /translation="MVGQCCVAVGLALLLGCAGTGEMVSLNLQTQQPFAQSSQTEPLK
                     IVIEPFEDLRTDRSKIGQRTHLGGGVTNFNVNGGAPGVTIAEALAETLRQRGWNRRGW
                     DARVVQAGVGVSGADIVIGGEIRDFSTNAKSRVFNTKLTGESRLVIKARNLADDSSTV
                     RNLQGEQTKLVFWFTSEDVEPLMSGLLQAGIEQFIPDTKIEGRTLKSAK"
BASE COUNT          459 a          728 c          613 g          393 t
ORIGIN      
        1 accctccctc ctctccctcc ttgttttttc ttggacccct tcgctatact tccgctttga
       61 tcaacgacaa ccggcagctc aacatggtca tccaacgagg ccgcagggga gaaagaaccg
      121 gaggcgtacc ctctgcggta cgttgaggat tctttcgaaa cgagaacgaa gttgggggcc
      181 atgttcagct gccgacgaca atacaggata ctcaaaacgg ttgtccggca aggccgcagc
      241 gagtgagagg gcgaggcgta ctctgcctcg tacgtcgagc cctggaacga agcgagaacg
      301 ccgccgggaa ccgttttcag gatcctgtca gatgaagggt gggagcggcg catacgtcac
      361 atcgtatccc aacagacgtt ccagccacgc agccatatgt tcttcaggca agggagcgcg
      421 attcaaacgg gcttccaact ggaatccacc ggcggtgccg acgatcccga taatggccga
      481 cgcatcgtcc ccttccggca tcaactcctg tgtttgaaat tcgcacagcg tgctatagag
      541 gcgtccctgc ggctcgatcg tggcgagcac ttccggcaat tcgcccagtg cgcaatgcac
      601 ggaacagcga tacaccgaac gggcgcctgg ctgtaccgcc gcaaatgcgt ccagcgccgg
      661 ctccgaaaaa cccgtcaggt aggcgcgcac actggccggc tgcggcgcaa aggtggccgc
      721 caaccggctc gccggcacga gaaactnnnn nnnnnngtag gcatccaagg agttgtattc
      781 gaactgacag gggccgaagg catccggcca ggaacagaaa aacggcgtcg ccggatccgc
      841 cggcctcaac accaggccgc ccttctccac ggaggtttcc accggcaggt ccaacgtccc
      901 gatagcgtcg agaaagatcg cccgccgttc atcgatccat tccacccgcc gcgcgtcctc
      961 acgcgtttcc ccgggcgtca ccacttcctg cgtatgcagg cgcacgcgga taaaggcatg
     1021 gttatgccgg aagcccagcc agaacatgca cgtgggatga tgaaaccgca gcccgggttt
     1081 cgtgcgccag ggatggctga tggagcagta ggtaccgacg tcctgaaacc gctggtaaac
     1141 cgtgtggtag ccgcctgcga gtagaaaaaa atcgccttgc caggcctcgc ggatatgcag
     1201 aagcgtgacg tcctccgtcg cccagtgatc gagttgatcg aacgcgtcgg gagaatcgcc
     1261 cgcccaatcc tccacgtagc aaatcacatc cttggcggga tgcgcgggct tcagtcccag
     1321 agtcgccagc cgctcgccga ccgccagcac ctcgcgaaag gtcaatcggc ccgaggtctc
     1381 ccaccgccgt tcacgcacaa ccaccctccg cactcgccac gcgacggccg aaaccggacc
     1441 gcctcatttg gctgacttca gcgtgcggcc ctcgattttg gtatcgggga taaactgctc
     1501 aatcccggct tgcaggaggc cggacatgag aggttcgaca tcttcgctgg tgaaccagaa
     1561 caccagcttg gtctgttcgc cctgaaggtt gcgcacggtg ctgctgtcgt ccgcgagatt
     1621 cctggctttg atgaccaacc ggctctcgcc ggtcaatttg gtattgaaaa cccggctctt
     1681 cgcgttcgtc gagaagtcac ggatttctcc cccgatcacg atatccgcgc ctgacactcc
     1741 gacgcccgcc tgtacgacgc gcgcgtccca accgcggcgg ttccatcccc gctggcgcag
     1801 cgtttcggcc agcgcctcgg caatcgtcac tcccggcgcg cctccgttga cgttgaaatt
     1861 cgtaacccca ccgcccaaat gggtacgctg gccgattttc gaccggtcgg ttcgcaaatc
     1921 ttcaaacggc tcgatcacaa tcttcaacgg ctcagtctgg ctgctctgcg cgaacggctg
     1981 ttgcgtctga agattcagcg agaccatttc tcccgtgccg gcacagccca gcagcaacgc
     2041 caggcccact gcgacacaac actgacccac catgcgacat ccctgtgcaa tcacacatgc
     2101 ctccctgtca gtaagtgacg ttccccaaag acgcgcacag tctacccaac tcgcagccgg
     2161 atgacaaacg ggaaggaggc ctggaagaaa cgcgtcggcg cgg
//
LOCUS       DAORQN010000235         2198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1393_length_2198_cov_48.580495, whole genome shotgun sequence.
ACCESSION   DAORQN010000235 DAORQN010000000
VERSION     DAORQN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_2198_cov_48.580495"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..561)
                     /locus_tag="PK614_09150"
     CDS             complement(10..561)
                     /locus_tag="PK614_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82410.1"
                     /translation="MIQLEGLRKEQKWTPGTLQGLIDFVHRVERQELASSSNKELADA
                     YKQLGKAIVTITRIRTAPPDLVFRLTELQYELMGEKPLELSWSQWRTWNLAPFFSGAK
                     KWPACEFFLEAIEAKQQVSAGAMKVAHSRMRSEYGSLPLDSQIAFLLDRMKGRRKRSR
                     ISIITNRSAYPRLQLALRRNSRA"
     gene            706..1092
                     /locus_tag="PK614_09155"
     CDS             706..1092
                     /locus_tag="PK614_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82411.1"
                     /translation="MQNFRLTFKGASEGAWHALRLLLALLIDLPLLSFGSGYRRGESK
                     PQRWVESTHGTSTHSSSVADWSIVGNRAVAYWKGLWHILGWFVGTIFPGSLLYTLLMT
                     AWILLWAAAILALPIWIGMVVWQALH"
BASE COUNT          484 a          616 c          587 g          511 t
ORIGIN      
        1 ctcttcgatt taggcgcggc tgtttctgcg gagagctagt tgcagtcgag ggtatgcaga
       61 gcggttggtg atgattgaga tccgggagcg tttacgacga cccttcatgc gatcgagtaa
      121 aaaggcgatt tgtgaatcta gcggcagaga accgtactcg ctcctcattc gtgaatgcgc
      181 taccttcatg gcccccgcgc tcacttgctg ttttgcttca atggcttcaa ggaagaactc
      241 gcaagccggc cacttcttag cgccgctgaa gaatggagca aggttccagg ttcgccactg
      301 tgaccacgaa agctcgagag gcttttcccc cattaattcg tattgaagtt cggtgagtct
      361 aaaaacaagg tccggtgggg cggttcgaat tctggtgatc gttacaatag cttttcccaa
      421 ttgtttgtaa gcgtcagcca gctccttatt tgagctagac gcaagttctt ggcgttctac
      481 ccggtgaacg aaatcgatca acccttgaag tgtgcctggt gtccattttt gttctttgcg
      541 gagaccttcg agttgaatca taaggcatgt cccatcctgt cgggtcctgg tgaggagaga
      601 ggcacgggcg ccaggaacgc ccgccggcca ggtgatcaag cccaaccgtt gcctcatgtc
      661 gaatgtgtat tctacacttg cacagccacc cagtgaacgg ccttcatgca gaattttcga
      721 ctgaccttca aaggcgcaag cgaaggagcc tggcatgctc tcagattgct tctcgccctg
      781 ttgatcgatc tcccactatt gagctttgga agcggctacc gtcgtggaga gagcaagccg
      841 cagcgatggg ttgaatctac tcacggaacg tcgacccatt cttcatcggt agcggattgg
      901 tccattgtcg ggaacagagc cgttgcctac tggaaaggcc tctggcatat tcttggatgg
      961 tttgttggga cgatctttcc gggctccctt ctgtataccc tgctcatgac ggcttggatt
     1021 ctgttgtggg ccgctgctat tcttgcgctg cccatctgga ttggtatggt tgtatggcaa
     1081 gcacttcact gaagccccct tcccgccgct accgcgggga caactactgg gaaaaagagg
     1141 cgccacttaa ggtgacgacc gacaaggttc ggatctgctt ttacccgcgt gctcagaaac
     1201 ttcagctatc tgccggttgg aaaaaccgtg acaccggaaa ggtcagatac ggccgcacgg
     1261 tagttctcga caccgcaagt cttacagcca gccctgaagc cctcgcctta ttcgaaacga
     1321 ttctagccac tgcccccgcg taggctcccc ttcgcaacag aaccgacagg aaccacctga
     1381 agtccagaat acacgtgctc ttctcgtatt ctctgaggct cgcggaatag ccgaaatgtg
     1441 tttatttgtc aggttccacg caagaagaca tgggcccata tatgcgtccg gaaccaaaaa
     1501 agcgatggta ccgcggtttt tgggagatca gtcagaggga ggaaggtatg acctacctac
     1561 cccccatccc cctgtttttc ccttaccctc ttcgctctct ccgaacctcc gagagtccga
     1621 ctccgggaaa ggtgcacctt cccccactca cccgcccacc tcttccctga ttcgaactca
     1681 tccggaaccg tgcgttttct tcccagattc aacagaagtt attggcagga cagttattga
     1741 ctccactgat cggagcgcat agggcccgga ccatgaggcg gcgcccgaga gcttgtccga
     1801 gaatctcccg ccttcaaacg gattcaagcc acagcaatcg aaaacaggtg cgatcaggcc
     1861 agactgaagg aagctgctgc gaggcgtctc taggggcaat gtgtgcgttg gtcaataact
     1921 gctattgact ccctcgactg aaaagggacg gcgccggacc agtgaacacg gttcctgaag
     1981 tcaataaaac gccccggacc gttcccgatc cgaggccgtt ctgcgggata cctttggctg
     2041 gttgcactac cagtcctgca cactccatga cctgaggtgt tcggtctccc gatcaaagac
     2101 cagctgaaga cgggagcccg attcgggcag cgccactgcc agggaagagg aataaggatt
     2161 accagggatc ggcggcatga tcagatagcc aggactcg
//
LOCUS       DAORQN010000236         2192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1397_length_2192_cov_4.094525, whole genome shotgun sequence.
ACCESSION   DAORQN010000236 DAORQN010000000
VERSION     DAORQN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_2192_cov_4.094525"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>452)
                     /locus_tag="PK614_09160"
     CDS             complement(131..>452)
                     /locus_tag="PK614_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82412.1"
                     /translation="FMFGKSGQSFDPILLKLFVNCVGIIPIGSLVRLDSGPLAVVIKP
                     AQDKTNAERPLVRVITDESGAPIEDGPELDLTEQDAAGHYTHSILQLVDNAEYRFDTG
                     RYFV"
     assembly_gap    453..476
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<477..>1523)
                     /locus_tag="PK614_09165"
     CDS             complement(<477..>1523)
                     /locus_tag="PK614_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRB82413.1"
                     /translation="DSMLTLVKTLAADHPVVLRLQNDFLFLGDSHLKMSSQQMAVFAS
                     IIEALNKWHIGGVSFASTADSKDFREFAYLFVSLDPDKHSLTDLQEAMQAAGIKGIEL
                     EDPRSLQLQHLNDAELAGGNTGARTSGSSNGSEGSKEKRKALAKGGYLKASAALGDLV
                     KNTRGGGTVSFKQAKRAIQNIVELMMQDESTLLGLTNLRCHDQYTHNHSVNVSLLAMA
                     LGNRAGYPKVELADLGLSALFHDVGKCAISLDVLNKPGEFTQEEWAVMRSHPIEGVFT
                     LVNSRGINNVPARMAAASFEHHMNYDYSGYPKLKVPWTQTVASRIITIADCYDAMTSS
                     RVYRREPMSPANVLK"
     assembly_gap    1524..1551
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1552..1765)
                     /locus_tag="PK614_09170"
     CDS             complement(<1552..1765)
                     /locus_tag="PK614_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82414.1"
                     /translation="MSETPSPANPVPTQDNAHPILTHERSLANKIAKGSEAGDILDQQ
                     LAMLGIQLVTQLNVLIKTSRIHGRTNA"
     gene            complement(1783..>2192)
                     /locus_tag="PK614_09175"
     CDS             complement(1783..>2192)
                     /locus_tag="PK614_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82415.1"
                     /translation="SDETVRLTAMKVLSSGQYSAGFTVWAPFVSADDFHDRSPAEKRA
                     IYLALKQTAADEAVPYWQGLLTEWYWTNRKKKEELALLAADILGKLATPAAVAALEIG
                     QKKGGAAVRQACTSALSVANRQHRQSIPSAANS"
BASE COUNT          396 a          607 c          671 g          466 t
ORIGIN      
        1 agcctgccga ggctgctcgc acgtgagacc gacgcaggcg tactcgcagt ccgtcgagga
       61 gaacgaacga gaacagcctc gccgggcgag agaatcggac gccggagcag gtattcttgg
      121 atagtccggg ctagacgaag taccggccgg tgtcgaagcg atattcggcg ttatcgacga
      181 gctgcaggat actgtgtgtg tagtgtccgg ccgcatcctg ttcggtgagg tcgagttcag
      241 gaccgtcctc gatgggtgcg ccgctctcat ccgtaatcac tcgcaccaac gggcgttcgg
      301 cattggtctt gtcctgcgcc ggcttgatga cgacggccag agggccggag tcgagtcgga
      361 cgagactgcc gatcgggatg atccccacgc agttgacgaa gagcttgagc aggatcggat
      421 cgaacgactg gccgctcttt ccgaacataa acnnnnnnnn nnnnnnnnnn nnnnnncttc
      481 agcacgttcg cgggggacat gggttcccgt ctgtacaccc gggaggatgt catcgcgtcg
      541 taacaatcgg cgatcgtgat gatccggctg gcgaccgttt gagtccaggg caccttcagt
      601 ttggggtatc ctgagtagtc gtagttcata tggtgctcga acgaggcggc cgccatcctc
      661 gccggcacat tgttgatgcc gcgggagttc acgagggtga acaccccttc aatcgggtgc
      721 gaccgcatca cggcccattc ttcctgtgtg aattctccgg gtttgttcaa cacgtccagg
      781 gagatggcgc atttcccaac gtcgtggaac agcgccgaca acccgaggtc agccagctcg
      841 actttcggat aaccagcccg gttaccgagg gccatggcca gcagtgagac gttgacggag
      901 tgattgtgcg tgtattgatc gtggcagcgc agattggtca gtccgaggag ggtcgattca
      961 tcctgcatca tcagctcgac gatgttctga atcgcgcgtt tggcctgttt gaagctgacg
     1021 gtcccgccgc cgcgcgtatt cttgaccagg tcgccgagcg cagccgatgc tttcagatat
     1081 ccgcctttgg ccagtgcctt ccgtttctct ttcgaaccct ccgagccgtt cgatgagcca
     1141 cttgtacgtg ccccggtatt gcctccagcc aactccgcgt cgttcagatg ctgaagttgc
     1201 agcgagcggg gatcttccag ttcgatgccc ttgatgcccg cggcctgcat ggcctcttgg
     1261 agatcggtga gggaatgctt gtccggatcc aggctgacga agagataagc aaactcccga
     1321 aaatcctttg agtcggccgt cgacgcgaac gacaccccgc cgatgtgcca cttgttcaag
     1381 gcttcgataa tgctcgcaaa cacggccatc tgttgcgagc tcattttcag gtgactgtct
     1441 cccagaaaca aaaaatcgtt ttgcagccgg aggacgaccg gatgatccgc cgcgagggtt
     1501 ttcaccaacg tgagcatgga gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggcattcg
     1561 ttcgtccatg aatccgcgat gtcttgatga ggacgttcaa ctgagtgacg agctgaatcc
     1621 ccagcatggc cagttgctgg tcgaggatgt ccccggcttc tgagcctttg gcgatcttat
     1681 tggccaggga ccgctcatgg gtgagaatcg gatgggcgtt gtcctgggtc gggaccggat
     1741 tggcagggga aggcgtctcg ctcacaggtc gtctcctcgc aatcaggaat tggctgccga
     1801 tgggatggac tgccggtgtt gccgattggc gacggagagc gcggaggtgc aggcctggcg
     1861 gacggctgcg ccgccctttt tctggccgat ttcgagcgcc gcaacggctg cgggtgtcgc
     1921 cagcttgccg agaatatccg ccgccagcaa ggccagttct tctttcttct tccggttggt
     1981 ccagtaccac tccgtcaaca gtccctgcca atagggcacg gcttcatcgg cggcagtctg
     2041 tttgagggcc aggtagatcg cccgcttttc ggccggtgat cggtcatgaa agtcatcggc
     2101 ggagacgaac ggggcccaga cggtgaaacc ggcgctgtac tgtccgctgg aaagcacctt
     2161 catggccgtg aggcggacgg tttcatcact gt
//
LOCUS       DAORQN010000237         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1401_length_2186_cov_4.710465, whole genome shotgun sequence.
ACCESSION   DAORQN010000237 DAORQN010000000
VERSION     DAORQN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_2186_cov_4.710465"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK614_09180"
     CDS             complement(<1..215)
                     /locus_tag="PK614_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB82416.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLGRMGIKTLIVQQTLALGGGIWS"
     gene            complement(355..>2186)
                     /gene="thiC"
                     /locus_tag="PK614_09185"
     CDS             complement(355..>2186)
                     /gene="thiC"
                     /locus_tag="PK614_09185"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRB82417.1"
                     /translation="ASRKVHVDGALPGVRVPMREISLTPTQSANGQGPTPNQPITIYD
                     TSGPYTDPNVTIDVRAGLPPLRRAWIESRRDTEELSQVTSQYGRQRAADPKLADLRFT
                     HIRKPLRAKAGHNVSQMHYAKKGIITPEMEFIAIRENQSRARAKELLASTNGHGGGVL
                     QHPGQAWGARIPNIITPEFVRDEVARGRAIIPNNINHPETEPMIIGRNFLVKINSNIG
                     NSAVASSIEEEVEKMIWSIRWGADTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQA
                     LEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRMTGIVSRGGS
                     IHAKWCLSHHQENFAYTHFEEICEIMKAYDVAFSLGDGLRPGSIADANDEAQFAELET
                     LGELTKIAWNHDVQVMIEGPGHVPMHMIQENMEKQLKECQEAPFYTLGPLTTDIAPGY
                     DHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPTKEDVKVGVVTYKIAAHAADLAKGHP
                     GAQARDNALSKARFEFRWEDQFNLSLDPDTARSYHDATLPDNAAKVAHFCSMCGPHFC
                     SMKITQDVRDYAAQKQLEEQQAIQIGMKEKSEEFKKAGSEIYL"
BASE COUNT          372 a          646 c          667 g          501 t
ORIGIN      
        1 gaccagatgc cgcctccgag ggccaaagtc tgttgcacga tgagggtctt gatgcccatc
       61 cggccgaggt cgtgggcgca gatgaggccg gagggcccgg cgccgacgat gataacgtcg
      121 ctctcgatca gctgatcgaa ctctttatag tactcccgcg caatctggcg ggtgatatcg
      181 cgctcgcgta atggcgccgg cttcggcttt cccatgctac cttccctctc agcggctgct
      241 gaacatgact cccggctttg ttctcggctc catccaatcc tcaacgtacc ccggagggta
      301 cgcctccggt ttgagctcgc ctgtggcctt gccggatagc catggtgatc agccttatag
      361 atagatttct gaaccggcct ttttgaattc ttctgatttt tctttcatgc cgatctggat
      421 ggcctgctgc tcttccagct gtttctgcgc ggcatagtcg cgaacgtcct gcgtgatctt
      481 cattgaacag aaatgcggtc cgcacatgct gcagaaatgc gcgaccttcg cggcattgtc
      541 cggcagcgtc gcatcgtgat acgagcgggc ggtgtccggg tccagcgaga ggttgaattg
      601 atcttcccag cggaactcaa agcgcgcttt tgataaggca ttgtcacggg cctgtgcgcc
      661 cggatggccc ttagccaagt ctgccgcatg ggcggcaatc ttgtacgtca cgacaccgac
      721 tttcacgtct tccttggtcg ggaggccgag gtgttctttc ggcgtgacgt aacagagcat
      781 cgcgcaacca taccagccga tcatcgccgc gccgatgccg gaggtgatgt ggtcataccc
      841 cggagcgata tccgtcgtga gtggccccag cgtgtagaac ggcgcctcct ggcattcctt
      901 cagctgcttc tccatgttct cttgaatcat atgcatcggc acatggccgg gaccttcgat
      961 catcacctgg acatcatggt tccacgcgat cttggtgagt tcgcccaagg tctccaactc
     1021 ggcaaattgc gcctcgtcgt tggcatcggc aatcgaaccg ggccgcaagc catcgcccag
     1081 actgaaggcc acgtcgtagg ccttcatgat ctcgcagatc tcctcgaaat gcgtgtaagc
     1141 gaagttttcc tgatggtgcg agaggcacca cttggcgtgg atcgagccgc cgcgcgagac
     1201 gatgccggtc atacgtttcg ccgtcatcgg cacatagcgc agcaggacgc cggcgtggat
     1261 cgtgaaatag tcgacgccct gttcggcctg ttcgatcagc gtatcccgga aaatttccca
     1321 ggtgaggtcc tcggccttgc cgttgacctt ttcgagcgcc tgatagatcg gcacggtacc
     1381 gatcggtacg ggcgagttcc ggatgatcca ctcgcgtgtc tcgtgaatgt tcttgccggt
     1441 cgagagatcc atcaccgtgt cggcgcccca acggatcgac cagatcatct tctcgacttc
     1501 ttcctcgatc gaggacgcga ccgcggaatt gccgatgttg gaattgatct tcacgaggaa
     1561 gttccggccg atgatcatcg gctcggtttc cgggtgattg atgttgttcg ggataatcgc
     1621 gcgtccgcgg gccacttcgt cgcgcacgaa ttcgggagtg atgatgtttg gaatacgcgc
     1681 gccccaggcc tggccgggat gctgcaatac gccgccgcca tggccgttgg tcgaggccag
     1741 gagttctttg gccctcgcgc gggactgatt ctcccggatc gcaatgaatt ccatttccgg
     1801 cgtgatgatg cccttcttgg catagtgcat ctggctgacg ttgtgtccgg ctttggcgcg
     1861 gagcggcttg cgaatatgcg taaaccggag gtcggccaat ttcgggtccg cggctcgctg
     1921 acgcccgtac tgggacgtga cctgcgaaag ttcttccgta tcccggcgac tctcgatcca
     1981 ggcccggcgc aagggcggca ggccggcgcg cacgtcgatc gttacgttcg gatcggtata
     2041 ggggccggag gtgtcgtaaa tcgtgatcgg ctggttgggc gtggggccct ggccgttggc
     2101 tgactgcgtg ggggtcaggc tgatttcgcg catcgggacc cggacgcccg ggagagcgcc
     2161 gtcaacatgc accttgcgcg aagccg
//
LOCUS       DAORQN010000238         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1405_length_2178_cov_5.586905, whole genome shotgun sequence.
ACCESSION   DAORQN010000238 DAORQN010000000
VERSION     DAORQN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1405_length_2178_cov_5.586905"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK614_09190"
     CDS             complement(<1..226)
                     /locus_tag="PK614_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82418.1"
                     /translation="MKNPRLIITLLAKQIPDCPDKLLYVRNFELRERAVREREPKVYT
                     DGISFGGTDLLPEVIDVNAASRDRFEKDLDQ"
     gene            complement(636..1733)
                     /locus_tag="PK614_09195"
     CDS             complement(636..1733)
                     /locus_tag="PK614_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB82419.1"
                     /translation="MKIEVRGLTKTFGSATAVGGVSFVVNEGELLGLLGPSGSGKTTV
                     LRLIAGLEVPTAGDIFIDGKRVNDLTVQERNIGFVFQHYALFKHLTVFDNIAFGLKIK
                     KWNRRDIEQRVTELLALMSLEGLGGRYPHQLSGGQRQRVAIARALAPRPSVLLMDEPF
                     GAVDAKVRQELREWLIRLHTDLNVTSLFVTHDQEEAMEVSGRIIVFSKGKLEQAGSPA
                     DVYEEPATEFVARFIGSMNIVAGVVRRGQVQVGSLEFPAPGFSDGQTLQVGFRPYYVK
                     VAEDPARYRQQAKLRHIYFLGVAYRLEIETPDGLILRSRMNKEEFRRCRFTVGQAVSF
                     AVTHFRILPQEGTSPSPGTEAGKPITGPLTP"
     gene            complement(1738..>2178)
                     /locus_tag="PK614_09200"
     CDS             complement(1738..>2178)
                     /locus_tag="PK614_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRB82420.1"
                     /translation="KVLFALPAMILATLFVTFTFMVRELTPLLQTLGTEEEEAARTLG
                     AGEWQVFLKVTLPALRWGLVYGATLTVARAIGEFGAVLVVSGNILLLTQTATLHIYQS
                     YVDFNYVAANAVALTLLAVSFAILTVLEIAKARAETTVAEAGAQ"
BASE COUNT          455 a          600 c          642 g          481 t
ORIGIN      
        1 tctgatcaag atccttttcg aatcggtccc tagaggctgc gttcacatct atgacctcag
       61 gaagcagatc tgttccccca aaactgatcc catccgtata aaccttgggc tctcgctctc
      121 gaacagcccg ttctcgcaac tcaaagtttc tgacgtacaa gagtttgtcg ggacagtcgg
      181 ggatttgctt ggcaagcaac gtaatgataa gccgtgggtt cttcaatggt ttgggatcct
      241 tcgaatctgg cgaaactccg actaacttgt gctctgcaag ccgagcaatc gtgtgggagt
      301 aaagctggct ggtgtcctgg ccagcgttgc gagtttgaac tgcgatgaga gcgtagattt
      361 cgacgttcga aaaaacgagg cctttgagtg cgggtacgtc tccccattca gaaccctgag
      421 cctgcgtgaa agggaatgca agcgcagcgg ttagcactat gaaaaagatt gaaagatttc
      481 gacatacatt ctgcatgact agttggttcc agccatctag acaatgggca ggcattgtgg
      541 gtcctcctta ggagtatggt gggaggggag tgcgctcgga aactctcacc aacctacttt
      601 ggttcgttga gtcagggttg tgccgctgtt ctctgctatg gcgtcagtgg cccggtgatc
      661 ggttttcctg cctctgtacc cggcgagggg ctcgtgcctt cctggggaag aatgcggaaa
      721 tgggtgacgg cgaaggagac ggcttggccc acggtgaagc ggcagcgccg aaattcttcc
      781 ttgttcatgc gggagcggag gatgagtcca tccggggttt caatttcgag ccggtaggcc
      841 acgccaagga agtagatgtg ccggagtttc gcctgttgcc gataccgcgc tggatcttcc
      901 gccaccttca cgtagtaggg acgaaatccc acttgcagcg tctgaccgtc ggaaaagccg
      961 ggggcaggga attcgagcga gccgacctgg acctgtccgc gccgcacaac gccggccacg
     1021 atattcatcg aaccgatgaa gcgggcgaca aactcagtgg cgggttcttc atacacatca
     1081 gcgggagatc cggcctgttc caatttgcct ttggagaaca cgatgatgcg gccggacact
     1141 tccatcgcct cttcctgatc gtgtgtcacg aacaggctgg tgacattcag atcggtatgc
     1201 aggcggatga gccactcgcg caactcctgc cgcaccttcg catcgacggc gccgaacggt
     1261 tcgtccatca gcaagaccga cggacgcggg gccagggcgc gggcgatggc gactcgctgt
     1321 cgttggccgc cggatagttg atgcggatac cgaccgccca gtccttcgag gctcatgagt
     1381 gcgagcagtt ctgtgacgcg ctgttcgatg tcacgccggt tccacttttt gattttgaga
     1441 ccgaaggcga tgttgtcgaa gaccgtcagg tgtttgaaga gagcgtaatg ctggaagacg
     1501 aagccgatat tacgttcctg tacggtgagg tcgttcaccc gtttgccgtc gatgaagata
     1561 tcgccggcgg tcggaacctc caggccggcg atcaatcgca gcaccgtggt ttttccgctg
     1621 ccgcttggtc cgagcagacc gagcaattct ccttcgttga cgacgaacga gacgcctccc
     1681 actgccgtgg ccgaaccgaa cgtcttcgtg agcccgcgta cttctatctt cactggctta
     1741 ctgcgctccc gcttccgcta ccgtcgtttc agcgcgtgcc ttggcgattt ccagcacggt
     1801 caagatggca aaggagacag ccaacagggt caatgcaacg gcgtttgccg ccacatagtt
     1861 gaaatcgacg taactttgat aaatgtgcaa tgtggcggtc tgcgtgagca acaaaatatt
     1921 ccccgagacc acgaggacgg ctccgaattc gccgatcgcg cgcgccacgg tgagggtggc
     1981 tccatagact agtccccatc ggagggccgg cagggtgacc ttgaggaaca cctgccattc
     2041 accggccccc aacgttcttg ccgcttcctc ttcttcggtg ccgagtgttt gcagcaaggg
     2101 cgtcagctca cgcaccataa atgtaaaggt gacgaacagg gtcgcgagaa tcatggccgg
     2161 cagggcaaac agcacctt
//
LOCUS       DAORQN010000239         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1412_length_2169_cov_6.406339, whole genome shotgun sequence.
ACCESSION   DAORQN010000239 DAORQN010000000
VERSION     DAORQN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_2169_cov_6.406339"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1110
                     /locus_tag="PK614_09205"
     CDS             181..1110
                     /locus_tag="PK614_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB82421.1"
                     /translation="MLTGVSLQTALPWLLSISTTVGIGVLRVGLVFVIGYIAIRFARL
                     GLQQLERVMLLTSDAKDQQSGTAQKRAATLAGILRTIALTAIWAIVIIESLQVVGLDI
                     APILAGAGILGLAVGFGAQNLVRDLISGFFIILEDQIRLGDVAVINGTGGQVETITFR
                     TISLRDFSGVVHVFPNGGINTLSNMSKDWSAFVLDMGVAYKEDTDRVVAVMQAVGEEL
                     RHDEELGSLMIEPIEVVGVENFADSAVTIRARIKTQPLEQWKVGREYRRRLKKAFDAQ
                     GIEIPFPHQTLCMGEASPPFKVEVVAGLGLQKV"
     gene            complement(1210..1380)
                     /locus_tag="PK614_09210"
     CDS             complement(1210..1380)
                     /locus_tag="PK614_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82422.1"
                     /translation="MITIIGLVTIVTLIWLLAWAMGLESDSERRKLISASSAAAPTHS
                     DIVPERQTRRAA"
     gene            complement(1497..>2169)
                     /locus_tag="PK614_09215"
     CDS             complement(1497..>2169)
                     /locus_tag="PK614_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82423.1"
                     /translation="GEAAFGIEQLVRDAEMYRIGEGATDILRPFVVREGLSPHLDRAK
                     RFYADGLSILEQARQAMKLMRFYLPWYLRQWRKRPLPDRRELTHPQVRPAILYVERTS
                     RRLARAIFYALLRFQASFKDEQRLQNKIESVGEDLLAMLATVLYAERQTRLDGHTSVW
                     ELAEAFSAAAKQRIEQRLREFRHHRDTTAATTGRQALKGYYPTLSEGIIRRRLEDYRR
                     NQHMT"
BASE COUNT          427 a          583 c          688 g          471 t
ORIGIN      
        1 gatgtcgcta gactgacagg cgtgctccac gactgacgga gccaacgtag ccattcatgt
       61 cggacgtcac gattccatcg gtgcccaagc tgttgcggca gcttctggcc gctctgcgcg
      121 gggagtggtg gtcccttcgc aagttgaaac gccggacgtc ctcacacaag ggagatcgat
      181 atgttgaccg gagtgtctct gcagacggcc cttccctggc tcctgagtat ttccacaacc
      241 gtaggcatcg gcgtgttgcg ggtcgggctg gtgttcgtga ttgggtatat cgcgattcga
      301 tttgcccgtt tggggttgca acaactcgaa cgagtgatgc ttctcacgag tgacgccaag
      361 gatcaacagt ccggaacggc tcaaaaacgc gccgcgacgc tcgcgggaat cctcaggacc
      421 atcgcgctca cggcgatttg ggcgattgtc atcatcgaat cactgcaggt ggtggggttg
      481 gacattgcgc cgattctggc cggtgccggt atcctcggtc tggccgtggg cttcggtgca
      541 cagaatctgg tgcgcgatct gatcagcgga ttcttcatca tcctcgaaga tcagatccgg
      601 ctgggagatg tggccgtgat caatggaaca ggcggacagg ttgagaccat cacgttccga
      661 acgatttcct tgagggactt ttccggcgtg gtccacgtgt ttcccaacgg cggcatcaac
      721 acgttgtcca atatgagcaa ggattggtcg gccttcgtgc tggacatggg agtggcctat
      781 aaagaggata cggatcgggt ggtggcggtg atgcaggcgg tcggtgaaga attgcgccac
      841 gacgaggaac ttggctcgct gatgatcgag ccgattgaag tggtgggtgt tgaaaatttt
      901 gccgatagtg ccgtgacgat tcgcgcgcgc atcaagacac agccgctcga acagtggaag
      961 gtcggacgtg aataccggcg acggctcaag aaggccttcg acgcgcaggg tatcgagatt
     1021 ccgttcccgc atcagacttt gtgtatggga gaggccagtc cgccgttcaa agtagaggtg
     1081 gttgcaggtc tggggcttca gaaggtctag caggatgcgg aaaaagtccg attgagtccg
     1141 atggccgtgc ttaccaggag catgaaccag gcgtctccgt ggttctgggc aaccggtggc
     1201 cggccgctac tatgccgccc ggcgagtctg ccgctcaggc acgatgtcgc tgtgagtggg
     1261 ggcagcggca gacgaggcgg agatcagttt tctccgttcc gagtcactct ctaatcccat
     1321 ggcccaggcc aacaaccaaa tgagtgtcac aattgtgacg agaccaatga tcgtaatcat
     1381 gtggcacctc acggctgaat gatacggaag ccgcacgggc cgcagtctgt ctcgatacgg
     1441 cctgccggct gctgagtaac gtctctcgaa ttcaccgggc gatgtgcggg cttcgctcat
     1501 gtcatgtgct ggttgcgacg atagtcctcc aagcgccggc gaatgatccc ctctgacagc
     1561 gtgggatagt accctttgag ggcttgccgc ccggtggtcg cggcagtggt gtcccggtga
     1621 tgccggaact ctctcaaccg ttgttcaatt cgctgtttcg ccgcagcaga gaacgcctcg
     1681 gccaattccc acacgctggt atgtccgtcg agcctggtct gtcgttcggc atagagcacc
     1741 gtggcgagca tggcaaggag atcttcaccg acggattcga ttttattctg cagacgctgc
     1801 tcgtctttaa atgaggcctg gaatcgcagc aaggcgtaga aaattgctcg cgccagccgc
     1861 cggctggttc gttccacata gagaatcgcc ggccgtacct gggggtgggt gagttctcga
     1921 cggtccggca gaggcctttt tcgccattgc cgcaggtacc agggcaggta gaagcgcatg
     1981 agcttcatcg cctgccgggc ttgttcgagt atggaaagac catccgcata gaagcgtttg
     2041 gcgcgatcca gatgagggct caagccttcg cggaccacaa aggggcgcaa gatatcggtg
     2101 gcgccttcgc cgattcgata catctccgca tcgcgaacga gttgttcgat gccgaaggcg
     2161 gcttcacca
//
LOCUS       DAORQN010000240         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1414_length_2159_cov_4.232414, whole genome shotgun sequence.
ACCESSION   DAORQN010000240 DAORQN010000000
VERSION     DAORQN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_2159_cov_4.232414"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1463)
                     /locus_tag="PK614_09220"
     CDS             complement(186..1463)
                     /locus_tag="PK614_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82424.1"
                     /translation="MARVLACGVGLVLCGLGGNVAQGEEPTPIAGEEFRTTLFGEEIY
                     VPPRDRRSVTAASFGIQSIPNGPSQMEMLPFGALYVWRNWDDDNRRLRGTFSGVVNDV
                     DYTIGLREYPNWSLIFTWDNFILPMGRSEYVQGQRQRGTELEWSYAFAGAGLGYRLPV
                     HPFRQDSAVNMSLTYEAGYRWFKGTGHTSAQYGVPKDTYEGRIHFRIRADALKRNLME
                     LPHEGFTVGGDLIHGHRAKWDQWGGAPFDTPDFKKEQTYLQASAYALVATGLPFIESE
                     KHRLVTSVHGGIGKDLDRFSAFRLPGRPTGYEWEAISLPMIHGVAFNELFPTRYAVAN
                     LQYRYEALFFLYPYLEATWGLVERPVFTSNGAVKNITDSMPALGGGLVTGAPWKSQVE
                     LNYTYNFGIYRDPGGAPPTKGGHGIIFFWSKQL"
     gene            1757..>2159
                     /locus_tag="PK614_09225"
     CDS             1757..>2159
                     /locus_tag="PK614_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82425.1"
                     /translation="MRLWQPQYNQSTSPPFGRRLALLALALAGMCLIATNEVLAAPIE
                     VIYPEGVSEGFVSKDGKKLANGELSQLTTGADRLASRLTFRFTDGSVYDETVTFSQKK
                     HLAMLSYQLNQRGPAFPEPLTISLNGETGQYQ"
BASE COUNT          473 a          641 c          577 g          468 t
ORIGIN      
        1 acccggccgc ataactggaa gtcatgctcc aggagttttt cgacaccttc agcaacgagc
       61 gtgtgatcat cggctatgag aactcttggt ttcatgggtg tgctccgggc tgagatgaga
      121 aggcggccag gaatggatgg aaaatcctac caccctctcg agacgatagc gaggggttcc
      181 tccggttaga gctgttttga ccagaaaaaa atgattccat ggcctccctt ggtcggcggc
      241 gcccctcctg gatctcgata aatgccgaaa ttataggtgt aattcagctc gacttgcgat
      301 ttccatggcg caccggtgac cagcccgccc cccagggccg gcatggaatc cgtaatattt
      361 ttgaccgcac cgttgctcgt gaagaccgga cgctcgacca gtccccatgt ggcttcaaga
      421 tacggataca ggaagaacaa ggcttcatat cgatactgca gattggcgac ggcataccga
      481 gtcgggaaca gctcgttgaa ggcgacgccg tggatcatgg ggagcgagat ggcttcccat
      541 tcatatccgg tcggccttcc cggcaaccga aaagctgaaa accgatccaa atccttgccg
      601 atgcctccat gtacactggt cacgagccgg tgcttctcgc tctcaatgaa cggaagcccg
      661 gtggccacca atgcataggc gctggcttga aggtacgtct gctctttctt gaaatcgggc
      721 gtgtcgaacg gcgccccacc ccattgatcc cacttcgccc ggtggccgtg aatcagatct
      781 ccccccacgg tgaacccttc gtggggcagt tccatcaagt ttcgctttaa cgcatcggcg
      841 cggattcgaa aatggatacg tccctcatac gtgtctttgg gcacaccata ctgggcagag
      901 gtatggccgg ttcctttgaa ccagcgatag cccgcttcat acgtaaggga catgttgacg
      961 gcgctgtcct gtcgaaacgg atgcacgggc aatcgatacc ctaaccctgc cccggcaaag
     1021 gcataactcc attccaattc cgttccccgt tggcgttggc cctggacata ttccgaacga
     1081 cccatgggaa gaatgaaatt gtcccaggtg aagatcagcg accagttcgg gtactcccgc
     1141 aggccgatcg tgtaatccac gtcattgacc acaccggaga acgtgccgcg gagtcgtcgg
     1201 ttgtcgtcgt cccaattacg ccacacatag agggcgccga acggcagcat ctccatttga
     1261 ctcggcccgt ttggaatcga ctgaatcccg aaactcgccg ccgtgacact tctgcgatcg
     1321 cgcggcggca catagatttc ttcgccgaat aaggtcgtcc gaaattcttc tccggcgatg
     1381 ggagtcggtt cctctccctg cgcaacattc cctccaaggc cacacagcac gagacccacg
     1441 ccgcatgcga gcactctggc caccttacgg aatcctcttc gatgcataac ggccatatcg
     1501 cgcctttctg ctctttcaca acagcgcctc agcgaatcac tcgtggtgtc tccttataag
     1561 acaatcgtga tatgactcgt cctagtaaaa cccctggata tgctgtccac atcggcgagt
     1621 acttacccgg tcgtgcgcgc cgatccccag gacgtcggaa agacctgcca tttcacctcc
     1681 gcagctagtg ggacaaagac taggtagatc tccagtatcg taatctgttg aggttcgata
     1741 gtctcggtgt gtgacaatgc ggctttggca acctcagtat aatcagtcga cttccccacc
     1801 gtttggccga aggcttgcgc tgttagcact ggccctcgcc ggaatgtgcc tgattgccac
     1861 gaacgaagtc cttgccgccc cgattgaagt catctacccc gagggtgtca gtgagggatt
     1921 cgtcagcaag gatggcaaga agctcgccaa tggggagttg agccagttga cgacgggggc
     1981 cgaccggttg gccagccgtc tcacgtttcg cttcaccgac ggatccgtct atgacgaaac
     2041 ggtgaccttt tcgcaaaaga agcatctggc catgttgagc tatcaattga accagcgggg
     2101 accggcgttc cctgaacccc tgacgatctc gctcaacgga gagacggggc agtaccagg
//
LOCUS       DAORQN010000241         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1422_length_2147_cov_3.524857, whole genome shotgun sequence.
ACCESSION   DAORQN010000241 DAORQN010000000
VERSION     DAORQN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_2147_cov_3.524857"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PK614_09230"
     CDS             complement(<1..223)
                     /locus_tag="PK614_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82426.1"
                     /translation="MASDNHVICYEAIQRTYRNSVVRFLRGKLCAKFPTEWERKLRAP
                     FKSEEWETTEKNALATRATGELGSEVGDDF"
     gene            340..>679
                     /locus_tag="PK614_09235"
     CDS             340..>679
                     /locus_tag="PK614_09235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB82427.1"
                     /translation="MPIYLNIDLRGRFLITLTTGKSGCYHPPMVRAERSPIHDQPEEA
                     AKAVSTWSGQAREQAVQRMFTAIAGVYDLNNTLLSFGLHHHWKRLTASYVPTVTNGRA
                     LDIGAGTADLA"
     assembly_gap    680..689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <690..1207
                     /locus_tag="PK614_09240"
     CDS             <690..1207
                     /locus_tag="PK614_09240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRB82428.1"
                     /translation="IEPKMGEQGHVIASDLNHAMLAEGLRKVTGRGLRDRITCLQANA
                     EQLGFPDETFHAVTTGFCMRNVGNLTQAFTEIRRVLRPGGRFVCLEFSRPAYGWLRGL
                     YDWYSFRLLPWIGTKVARDRTGVYEYLPASIRTFPDQERLATLLREAGFRQVEYRNLT
                     GGIVAIHIATK"
     gene            1303..>1787
                     /locus_tag="PK614_09245"
     CDS             1303..>1787
                     /locus_tag="PK614_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB82429.1"
                     /translation="MESILYIFLPCKKVYPIGVTYLADFIHRRKPEVRQRILDLSLFS
                     ESQRSQAIRDAALEFKPDLVCFSWRDIQIFSPHEGDASLEHAFNFYFAGNPIKRVAAS
                     FAGLKQLYRYYSHIRANLSYPWLIRKEFPKTQIMIGGGAFTAFADQLIEKLPEGTIGI
                     LG"
     assembly_gap    1788..1804
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1805..2085
                     /locus_tag="PK614_09250"
     CDS             <1805..2085
                     /locus_tag="PK614_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB82430.1"
                     /translation="ENERYIIREGKQTRKGNQGSPALLDALTVDLPYLTSIFPQHAAY
                     MDESIGVQTKRGCPYDCAFCLYPYIEGKRVRYRPPEMVVKDISQHYHQ"
BASE COUNT          452 a          634 c          559 g          475 t
ORIGIN      
        1 caaagtcatc acctacttct gaacctagtt ctcccgtagc tctagtagcg agtgcattct
       61 tttctgtagt ttcccattct tccgacttga agggagctcg cagtttacgc tcccattcgg
      121 ttgggaattt cgcacaaagc tttccgcgca gaaatcgaac tactgagttt ctgtaagttc
      181 gctgaattgc ctcataacag atcacatgat tgtcgctagc catttcttct ccctaccaaa
      241 atcgtcggac tgagggcggt cggcagatat ggatttatca gcgagtcaag tataagactt
      301 ttgagcaacc ttcggcgagt ttgatgcttt gacattaaag tgccaatcta tctcaacatc
      361 gatttgcgcg ggcgcttctt gatcaccttg acaacaggca aaagcgggtg ctaccaccct
      421 cccatggtgc gggcggaacg atctcccata catgatcagc cggaagaggc ggcaaaagcc
      481 gtctcaacct ggtccgggca ggcgcgtgaa caggccgtcc aacggatgtt caccgcgatc
      541 gcgggcgtct acgatctcaa caacaccctg ctgagtttcg gccttcatca ccattggaaa
      601 cgcctcaccg cgtcgtacgt gcccaccgta acgaacggac gcgcactcga catcggcgcc
      661 ggcacggcgg atctcgcccn nnnnnnnnnt gattgaaccc aaaatgggcg agcagggcca
      721 cgtcatcgcc tccgatttga accatgcgat gttggcggaa ggactgagga aagtcaccgg
      781 gcgcgggctc cgcgaccgga ttacctgcct gcaggccaat gccgagcagc tgggcttccc
      841 tgacgagacc ttccatgccg tgaccaccgg cttctgcatg cgcaacgtgg gcaacctgac
      901 gcaggcgttc acggaaatcc gtcgggtgct gcggccgggc ggtcgattcg tctgcctgga
      961 gttttcacga ccggcctatg gctggctgcg cggcctctac gattggtatt cgtttcgtct
     1021 acttccctgg atcggcacca aggtcgcacg agaccggact ggggtctacg aatacctccc
     1081 ggcgtcgatc cgcacctttc ccgatcagga gcggttggcc accctgttac gcgaagccgg
     1141 atttcgccag gtggagtacc ggaacctcac cgggggcatt gtcgccatcc acatcgcaac
     1201 caagtaacaa agagcgtatg gcctatggcg aatagcacca ggagcaggga acaattcctt
     1261 tctcacctgt cagccatacg ctattagcca tcagctccga ccatggaatc cattctctat
     1321 atttttctcc cctgcaagaa ggtgtacccg atcggggtga cctatctggc cgacttcatc
     1381 catcgccgga aaccggaagt gcggcaacgc attctcgacc tctcgttgtt ttcagagtca
     1441 cagcgcagcc aggcgattcg cgatgccgcg cttgagttca agccggacct tgtgtgtttt
     1501 tcgtggcggg acattcagat tttctctccc catgaaggcg atgcctcgct ggagcatgcc
     1561 ttcaatttct atttcgccgg gaaccccatc aaacgggtgg cggcgtcgtt cgccgggctg
     1621 aagcagctct atcgctacta cagccacatt cgcgccaact tgtcgtaccc ctggctgatc
     1681 cggaaggaat tccctaaaac gcagatcatg atcggcggcg gagccttcac ggcctttgcg
     1741 gatcagttga tcgaaaaact gccggaagga accatcggca ttttaggnnn nnnnnnnnnn
     1801 nnnntggaaa acgaacgcta tatcattcgc gaggggaaac agacgcgcaa gggcaaccag
     1861 ggctcgccag cgctgctcga tgccttgacc gtcgatctgc cctatctcac ctccattttc
     1921 cctcagcacg ccgcctatat ggatgaatca atcggcgtgc agacgaaacg tggctgcccg
     1981 tacgactgcg ccttctgtct gtatccttat attgaaggca aacgcgtgcg gtaccggccg
     2041 ccggaaatgg tcgtcaagga tatctctcag cactatcatc aatagggtgc ccggcgtttc
     2101 tggtttaccg atgcccagtt cattaccggg aaggaagcct atccgca
//
LOCUS       DAORQN010000242         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1424_length_2146_cov_6.367288, whole genome shotgun sequence.
ACCESSION   DAORQN010000242 DAORQN010000000
VERSION     DAORQN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_2146_cov_6.367288"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..996)
                     /locus_tag="PK614_09255"
     CDS             complement(307..996)
                     /locus_tag="PK614_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRB82431.1"
                     /translation="MRLRVASYNIHRAIGRDGRYEPGRILRVAQELNADIIALQEVDF
                     PRQDNAPIAPWLAKNTGMIAVAGPVWSREGVGYGNALLSRYPVDRVRRWDLSVGRHEP
                     RGALDVDLTICGRTVHVLNTHLGLWPRERPQQADRLLELLALSRRDLTILMGDLNEWH
                     VWGKSLRGLRRVFGSPAAPVTFPARWPVLALDRIWISPAQALLTVEAHNTPRSRLASD
                     HLPVKADLYLR"
     gene            complement(1018..2004)
                     /locus_tag="PK614_09260"
     CDS             complement(1018..2004)
                     /locus_tag="PK614_09260"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HRB82432.1"
                     /translation="MMQVDNMTLAVPLEQLQDSDALLSREWLVTNGLGGYASGTLLGA
                     PTRRYHGMFVPDLPSPWGRTVMIPRLDEEVVVEGESVLLSGIEFEDGRLQGDLTKVIN
                     AFTREWQTPVWRCSFKGRQLEKRIIMPYGHNSVYVEYRLLEGDPVRLHLRPFVTFRML
                     DAPLHEARTPPFPLIGAPSALAGALLSAMTIFCLGRALGRYRVQRFAGRRVAHLSRRI
                     AQRGFWAILIVRILPIAPFSMVNLVAGATPLSLRDFLLGTALGMSPGIVLMSAFVDRL
                     AEALRNPSPLAFGVLGLLMGAAWSFAWYVSRRLQVRGTAPDAGPGQRVALAV"
BASE COUNT          460 a          681 c          616 g          389 t
ORIGIN      
        1 cacgagtcag gcggggaacg ttggacgcgt atctcaacac ccatcatctg accagcgggg
       61 actatcggtt gacccatacc tattgcccat cctgttcagt tcgattttcg aaactgagcc
      121 gtgagcatgc ggccaccccg agagcctcgg caagtcgttc gctgtgaaac ggaagatgtg
      181 aaacgtatct cggagaactt cgtccgacgg attattaagc aacctgctcg gtaacggcct
      241 cggcaatggg cgacgtttca cgttttacgt cccacatttc acgcctcacg ttttacgttt
      301 cacgtctcaa cgaaggtaaa ggtcggcctt gaccggcaga tggtctgagg ccaatcggct
      361 ccggggagta ttgtgcgctt ccacagtgag cagcgcctgg gcgggagaga tccagatccg
      421 gtccagcgcc aacaccggcc atcgagcggg aaaggtgacc ggagcggccg gggaaccgaa
      481 cacccgacgc aatccccgca aggattttcc ccacacgtgc cactcattca ggtcacccat
      541 caaaatagtg agatcccgcc ggctgagtgc caggagttcc aacaatcgat ccgcttgctg
      601 cggccgttcg cgcggccaca gtcccaggtg ggtattgagc acgtgaaccg ttcggccgca
      661 gattgtgagg tcgacatcca gcgcgcctct cggttcgtgg cgacccaccg agagatccca
      721 acgtcgtacc cggtccaccg ggtaccggct caacagcgcg ttgccatacc ccacgccttc
      781 ccgtgaccac actggtccgg ccaccgcaat cataccggtg ttcttcgcca gccagggcgc
      841 aatcggagcg ttgtcctgcc ttggaaaatc aacttcctgc aaagcaatga tgtcggcgtt
      901 gagttcttgg gccacacgaa ggatgcggcc gggctcatat cgcccgtccc tgccgatggc
      961 gcgatggata ttgtaggagg ccacccgcag gcgcatcgtt ctcctgttca cgcggcgtca
     1021 gacagcgaga gccaccctct gaccgggacc ggcatccggt gcagtgcccc tgacctgtaa
     1081 ccgccgcgac acgtaccagg cgaaactcca tgctgcgccc atgagcaggc ccagcacgcc
     1141 aaaggcgaga ggactcggat tgcgaagcgc ttctgccaac cgatcgacga aggctgacat
     1201 cagcacaatt ccgggactca tgccgagcgc ggttcccaac agaaaatcgc ggagggacag
     1261 cggggtcgcc ccggcaacca gattcaccat agaaaatggc gcaatgggca ggatcctgac
     1321 gatcaggatt gcccagaacc ctcgctgggc gatccggcgg ctcaaatgcg ccactcgacg
     1381 gccggcaaat cgctggactc gatacctccc cagggcccgg cccaggcaaa aaatagtcat
     1441 ggcgcttaac agtgcgccgg ccagggcgga tggagcaccg atcagcggaa agggaggggt
     1501 tctggcttca tggagcggcg catccagcat gcgaaaggtc acgaacgggc ggagatgaag
     1561 acggaccgga tcaccctcca gcaaccggta ttccacatag actgagttgt gaccgtacgg
     1621 catgatgata cgcttttcca gctgccgtcc cttgaatgag cagcgccaga caggagtttg
     1681 ccactcgcgg gtaaacgcgt tgatgacctt cgtcaggtca ccttgcaagc gtccatcttc
     1741 aaactcgatg ccgctgagga gcacggactc tccctccacc acgacttctt cgtccaggcg
     1801 gggaatcatg actgtccgcc cccaaggcga gggaagatcg ggcacgaaca tcccgtggta
     1861 acgccgagtc ggggcgccga gcaatgtccc ggaagcatag ccgccgaggc cattcgtcac
     1921 cagccattct ctggagagca gggcatcgct gtcctgaagc tgctctagag gcactgcgag
     1981 agtcatattg tcgacttgca tcatggctct accgttccta ctcgcccctc ccgagaatgc
     2041 catcccgttc ttaaccttgg acggaataca acgctgccga tccgcccgga atgacccatc
     2101 cggtttcggt caagggctcg atgactccgg gtcctccgta ttccgg
//
LOCUS       DAORQN010000243         2141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1425_length_2141_cov_7.710930, whole genome shotgun sequence.
ACCESSION   DAORQN010000243 DAORQN010000000
VERSION     DAORQN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_2141_cov_7.710930"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1050)
                     /gene="pssA"
                     /locus_tag="PK614_09265"
     CDS             complement(250..1050)
                     /gene="pssA"
                     /locus_tag="PK614_09265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRB82433.1"
                     /translation="MKSPAMRAPFAKGNRKRQAMYLIPNLCTTGNLFCGVFAILSVFN
                     GQHLVAAIAILVGMIFDMLDGKLARLTNSTGQFGIEYDSLSDVVSFGVAPGVLIYSYA
                     LSGQGMFGVAVMFAYVAMGAVRLARFNATVSTSDSKYFTGLAIPAAAGVIASLVIFDL
                     HITQMGSEVKPLLILVITFALAFLMVSTIKYRSFKDMKFRRGDHFTYLVWGILALMLI
                     VAWPQAMLFVTFTGYAVSGPLARLWTMVSKSAGKPVAKTDTPVLDSRE"
     gene            complement(1047..1697)
                     /locus_tag="PK614_09270"
     CDS             complement(1047..1697)
                     /locus_tag="PK614_09270"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRB82434.1"
                     /translation="MADRAVGIPIVKEGLPFVGGLVGATLLFGAAGWIIPALIAGGFT
                     LFTAWFFRNPSRTIPQQPNVVVSSGDGKVIAIEEEFEPRYLKEKSIRVTVFLNVFDVH
                     VNRMPCAGTVEGVSYQPGQFLVASKPEATLRNEQNAVMLKTESGAKVLCVQVAGLIAR
                     RIVCWVGQGDRVERGERFGLIRFGSRMDTFLPLGSKICVSLGDRVKGGETIVGELR"
     gene            complement(1749..>2141)
                     /locus_tag="PK614_09275"
     CDS             complement(1749..>2141)
                     /locus_tag="PK614_09275"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRB82435.1"
                     /translation="GFETLVEAGYSPEMAYFECLHEVKLIVDLIYQGGIANMRYSIST
                     TAKYGDITRGPRVVTDQTKQEMKKILGEIQSGQFAKEWVLENQANRPVYNALLKKGEG
                     HPIEEVGARLRSMMPWLKKDQLVDKNKN"
BASE COUNT          474 a          714 c          527 g          426 t
ORIGIN      
        1 cggtggtacg acggacatcg cggtcatctc cctgggcggc atcgtgtata gcgagggatg
       61 aagcatgtcc ttcatccgaa cgcccttcat tcaatccgtg acgcggatca ttcggaaccc
      121 attacaaccc cggggggctt cacaagtcca gctcagaggc gagttcggca gatcaccggc
      181 tggttcgcac catccaccag ctctctcaat tcggtcagcg ctgcctacta ctcctcgtcg
      241 tcgctgttgt tactccctcg aatcgagtac cggcgtgtcc gttttcgcca ccggcttccc
      301 tgcactcttg ctcaccatcg tccacagccg cgccaatgga ccggacacgg cgtacccggt
      361 aaacgtcacg aacaacatcg cctggggcca ggcgacaatc agcatcaagg cgagaattcc
      421 ccacaccaga taggtgaaat gatccccacg ccggaacttc atatccttga agctgcgata
      481 cttgatcgta ctgaccatca agaacgccag cgcgaacgtg atcacgagga tcaacagcgg
      541 cttcacttcc gagcccatct gggtgatgtg cagatcgaag atcaccaacg acgcaatgac
      601 accggctgcg gcaggaatag ccaagcccgt gaagtatttg ctgtcggagg tgctcaccgt
      661 cgcattgaac cgggccaggc gaaccgcccc catggccaca taggcaaaca tcactgcgac
      721 cccgaacatg ccctggccgc tcagcgcata ggaataaatc agcacgccag gtgcgacccc
      781 gaacgacacc acgtcggaaa gagaatcata ctcgatgccg aactggccgg tactgttcgt
      841 cagtcgggcc aacttcccgt ccaacatgtc gaaaatcatg ccgaccagaa tggcgatggc
      901 tgccaccaga tgctgcccgt taaacaccga caggatcgcg aacacgccgc agaacagatt
      961 tcccgtcgtg cagaggttcg ggatcaggta catggcctgt cgctttcgat tacccttcgc
     1021 aaacggagct cgcatggccg gtgatttcat cggagttccc ccacgatggt ttcgccccct
     1081 ttgacgcggt cacccaagga gacacaaatc ttcgagccga gcggaaggaa ggtatccatc
     1141 cgcgacccaa accgtatcag cccaaaccgc tctccccgct ccacacgatc accctgcccg
     1201 acccagcaca cgatccggcg ggcaatcagc ccggcaacct gcacacatag cactttcgcc
     1261 cctgactccg tcttcaacat cacggcattc tgttcgtttc tgagcgtcgc ctccggcttg
     1321 ctcgccacga gaaactggcc gggctggtaa ctcacaccct ccaccgtccc ggcgcagggc
     1381 atccggttca catgcacatc gaagacgttc aagaacaccg tcacacgaat gctcttttcc
     1441 ttcaggtagc gcggctcgaa ctcttcctca atcgcaatga ctttgccatc ccccgacgac
     1501 accaccacat tcggctgctg cggaatggtc cgactcgggt tccgaaagaa ccacgccgta
     1561 aacagcgtga acccgcccgc aatcaaggcc ggaatgatcc aaccggccgc tccgaacagc
     1621 agcgtcgcgc ccacaagacc accgacaaac ggcaagcctt cttttacgat cggtatcccg
     1681 acggctcgat cagccataga cctccaggcc ttcttaatta tgctatcacg aagagcccgg
     1741 tcgcagggtt agtttttgtt cttatcgacg agctgatctt ttttcaacca gggcatcatc
     1801 gagcgaagtc gggcgccgac ttcttcaatc ggatggccct cgcccttttt tagcagggcg
     1861 ttgtagaccg gccggttcgc ctggttctcc aacacccatt ctttcgcgaa ctgaccgctc
     1921 tggatctcgc cgagaatctt cttcatttcc tgcttggtct gatccgtcac cacgcgcggg
     1981 ccgcgggtga tgtcgccata ctttgccgtg gtgctgatcg aatagcgcat gttggcgatg
     2041 ccgccctgat agatcaggtc cacgattaat ttcacttcat gcagacactc gaaataggcc
     2101 atctcaggcg aatacccggc ctcgacgagc gtctcgaacc c
//
LOCUS       DAORQN010000244         2137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1428_length_2137_cov_3.884726, whole genome shotgun sequence.
ACCESSION   DAORQN010000244 DAORQN010000000
VERSION     DAORQN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1428_length_2137_cov_3.884726"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK614_09280"
     CDS             complement(<1..570)
                     /locus_tag="PK614_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82436.1"
                     /translation="MYDGTEQDLLRLLASRAGISPEYHDIAGTLHVTSDETRRAILSA
                     MGFQVGSGAVLSQELTALDEAPWQQPCDPILILQQGDGPTQWTFRPALEEGEEQHVAV
                     AWSLTDEKGTIVHREETSPGLNPCEVRSLAGRRMVRVELPLVQNLGLGYYDLSVGTMG
                     SKVVTRGTLRVVVAPPHCFVPEELARGGRV"
     gene            869..1600
                     /locus_tag="PK614_09285"
     CDS             869..1600
                     /locus_tag="PK614_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82437.1"
                     /translation="MTPQLSRSGSGTLLAIAAQCLVFAATAQALPEHSRVDVTGDYRY
                     AYHEPETASEARQHACTEALYQAISTSAAVREQTASIVDSKLFRNLVHTLASKHVTDQ
                     QIVQQSETGRTVYCKVKAVFHADEVERVMIAQTTGGTEPGLDQNRALKIVSAREEADG
                     TLVIVYQALKRLDWLGTAYQGSLRESADVMVDFYDDQGVLVRSSRHPARKTATGDDVM
                     NPGEVGTLRIAKPLNAKTYRVWVVK"
     gene            complement(1686..>2137)
                     /locus_tag="PK614_09290"
     CDS             complement(1686..>2137)
                     /locus_tag="PK614_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82438.1"
                     /translation="EEVQRYPLFQLTTDPTTRTKLQDAIAREVQNKLIDKQNMPIRLN
                     RVVVGSILPPKGVVEQTTQTIVQEQRKITMVEFQKAEEAREKAEKQRGIADRAYRESL
                     GLTAPEFVDLRRIEVQKEIVQHSPSALTVIMGLERVGINMPPLASDR"
BASE COUNT          432 a          660 c          614 g          431 t
ORIGIN      
        1 cactcttcca ccccgggcca gctcctccgg cacaaaacaa tgcggcgggg ccacgaccac
       61 gcggagcgta cccctggtca ccaccttgct gcccatggtg ccgaccgaca aatcgtaata
      121 gccgagcccc aggttctgaa ccaacggcag ttcaacgcgc accatgcgcc gcccggccag
      181 cgaacggacc tcgcatggat tcaggccggg gctggtttct tcgcgatgaa cgatggtccc
      241 tttttcatcc gtgagactcc aggctaccgc gacgtgttgc tcctcccctt cctccagcgc
      301 ggggcggaat gtccactgcg tggggccatc cccttgctgg agaatgagaa tcggatcaca
      361 gggttgttgc cagggcgcct cgtccaaggc ggtcagttcc tgtgacagta ccgcgcccga
      421 cccgacttga aaacccatgg cggacagaat ggctcgacgc gtttcgtcac tggtcacgtg
      481 gagcgtaccg gcgatgtcgt gatattcggg tgaaataccg gcccgtgagg cgaggagacg
      541 aagcaggtct tgctcggtgc catcatacat agtgtcgtgc acgagtgtga gtgaagacct
      601 ccatcaagtc aaggtatcct atgcttttcc gcagagttgc gacatgactc acgccggtcg
      661 agttcgcgac tgagcatcga tccgacgcat tacccttcac gcgacgaggc tcatgactga
      721 ttgatagcgg ttccgttccg ggcacgagtg ccgcgcggcg tcgcattgct gcgccccctc
      781 acgcgtggta gcataacctt agcgtcgaga ggcgacgact gtctttgctc atcaggtgga
      841 accgggcacg gaaagggagg tggctcgtat gaccccgcag ctgtcgcgtt caggaagcgg
      901 taccttgctc gcaatcgccg ctcaatgtct cgtgtttgcc gcaacggccc aggccttgcc
      961 cgaacactcg cgggtcgatg tcaccgggga ttatcggtac gcctatcacg agccggaaac
     1021 cgcatcagaa gcgaggcaac atgcctgcac cgaagcgctc tatcaggcaa tcagcacctc
     1081 agcggcggtg cgagagcaga ccgcctcgat tgtcgactcg aaattattcc gcaatctggt
     1141 gcataccctc gcctcgaagc atgtgaccga tcaacagatc gttcagcaga gcgaaacggg
     1201 acgcacggtg tactgtaagg tgaaggccgt cttccacgca gatgaggtgg aacgggtgat
     1261 gatcgcacag acaacgggcg gaaccgaacc cggcttggat cagaatcggg cgctgaagat
     1321 cgtcagcgcg cgcgaagagg ctgatggcac actcgtcatc gtgtaccagg cgctcaagcg
     1381 tctcgattgg ctgggaaccg cctaccaagg cagcttgcgc gaatcagcgg atgtgatggt
     1441 ggacttctat gacgatcaag gcgtcctggt acggagcagt cgtcatccgg ccaggaaaac
     1501 cgcgaccggt gacgatgtta tgaatccagg cgaggtgggc actcttcgaa tcgcgaaacc
     1561 gctcaatgcg aaaacctacc gtgtctgggt ggtgaagtag tccaccgagg ctccggggac
     1621 aggaccggat gtcttccgct cctgtccccg tcccaatact cccaacacat ttccctccgt
     1681 gagtcttacc gatcgctcgc caggggcggc atgttaatgc cgacacgttc caatcccatg
     1741 atcaccgtca aggccgacgg cgagtgctgc acgatttcct tctgcacttc gatacggcgc
     1801 agatcgacga attccggcgc cgtcagtccc aacgattccc gataggcgcg atcggcgatc
     1861 ccgcgctgct tttcggcttt ttcacgcgcc tcttccgcct tttggaactc gaccatcgtg
     1921 atcttgcgct gctcctgaac aatggtttga gtcgtctgct cgacgacgcc cttcggcggc
     1981 aagatactcc cgacaacgac tcgattgagc cgaatcggca tattctgctt atcgatgagc
     2041 ttattctgca cctcacgcgc aatggcatcc tgcaacttcg tcctcgtcgt gggatcggtc
     2101 gtcaattgaa agagcgggta ccgttgcacc tcttccc
//
LOCUS       DAORQN010000245         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1432_length_2135_cov_5.774038, whole genome shotgun sequence.
ACCESSION   DAORQN010000245 DAORQN010000000
VERSION     DAORQN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1432_length_2135_cov_5.774038"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PK614_09295"
     CDS             <1..362
                     /locus_tag="PK614_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82439.1"
                     /translation="SLQVTVTRDIFPSGFGLLLQTDRVGALASYVLSDTLTASLDASG
                     YIVSGVTSQARGGTLPEQRLFYLTPKLAWHFSEWWRTEISYSYNWRDVETFNEPVMSN
                     TLMIMLTYYPPKLAFSN"
     gene            475..>2135
                     /locus_tag="PK614_09300"
     CDS             475..>2135
                     /locus_tag="PK614_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82440.1"
                     /translation="MVFFAAGFLLCLSLAAAVLWPPTFKSTATILIEEQEVPAELVHS
                     TITSYADQRIEMIKQQVMSRASLWKVVEQYDLYPEMRQESPTEEVIKRFIKDIEVEVI
                     SADVIDKRTQHATKATIAFTVSYNSRTAETAQRVANELTSLFLGENLKSRERQAQETT
                     TFLKQEAESLAAHIEEVEAKLSKFKQRAAGALPELMPLNLQMMNQADRELMDLDQQIR
                     SLEERKSYLEGELATIKPNTPILSVTGERILDSVERLRSLRAEYAGVAANLSPDHPDV
                     IKMKQEITALERDTGANPETEEIAKQLTDARARQATLADRLGQDHPDVLQTKRTIVAL
                     EREFRRIGTSAGNRPLQRPENPAYINIQAQLNSVNSSLQALKTSRATVKHRLQDYAQR
                     IERTPALEPDYLTLARDRDTSAQKYQDIRSRLLEAKVSEGLEVQRKGERFSLIDPPGL
                     PESPEKPNRKAIVLLGFILALAGGAGAAALTEHLDHSIRTPEQLVRVSQAFPLAVIPY
                     MPNRADLARALARRNRIRLAGLGTVALLLLICHLFWTPLDVVWYAT"
BASE COUNT          516 a          596 c          585 g          438 t
ORIGIN      
        1 ccagtctgca ggtgactgta actcgtgata tctttccgag cgggtttggc ctcttactcc
       61 agactgatcg tgttggagcg ttggcctcct atgtcttatc agacaccttg acggcgtctc
      121 tggatgcttc agggtatatc gtgtcagggg tgaccagtca ggctcgtgga ggcacgcttc
      181 cagagcagcg attgttctac ctcacgccga aactggcatg gcacttttcg gaatggtggc
      241 ggaccgaaat ttcctacagt tataactggc gcgatgtgga aacctttaat gaaccggtta
      301 tgtcgaatac cctcatgatc atgttgacat attatccacc gaaactggca ttttccaatt
      361 gagcatgttc cacatttaag gggaccttct atgccggatc acgggcgccc gcaagaacca
      421 gaatccacgg tcaatctgag agactatgtt gcaatattca accgtcggag aggcgtggtt
      481 tttttcgcag cggggtttct cttatgtctc agcctagccg cagcagttct ctggcctccg
      541 accttcaaat ccactgcgac gattctgatc gaagaacaag aagtgccggc tgagctggtc
      601 cacagcacaa tcaccagtta tgccgaccaa cggattgaaa tgatcaagca acaagtcatg
      661 agtcgtgcca gtctctggaa ggtcgtcgag caatatgacc tttatccgga gatgcggcag
      721 gaaagcccga ctgaagaagt catcaagcgc ttcatcaaag atattgaagt tgaggtgatc
      781 agcgctgatg tgatcgacaa acggacacaa catgccacca aggccacgat tgcttttacc
      841 gtatcttaca acagtagaac ggctgaaacg gcccaacggg tggccaatga gttgacgagc
      901 ctgtttctcg gcgagaatct caaaagccgc gaacggcagg cgcaggaaac caccacgttc
      961 ctcaagcagg aggcggagag cctcgcggcc catatcgaag aggttgaagc caagttatcc
     1021 aagttcaaac aacgagcggc aggtgcgttg cccgagttaa tgcccctgaa tctccagatg
     1081 atgaaccagg ccgatcgtga actgatggac ctcgaccagc aaatacggag cttggaagag
     1141 cgtaagagct atctggaagg ggaactggcg accatcaaac cgaacacgcc gattctctcc
     1201 gtgaccgggg agcgcattct tgatagcgtg gagagattac gcagcctccg ggccgaatac
     1261 gccggagtgg ccgcgaatct ttcacctgat catccggacg tgattaagat gaaacaggaa
     1321 attacagcgc tggaaagaga caccggtgcg aacccggaaa ccgaagagat cgccaaacaa
     1381 ttgaccgatg cccgggccag acaggccacg ctcgccgacc gcctcgggca ggatcatccg
     1441 gacgtgctcc agacgaaacg cacgatcgtc gcgctggaac gggaattccg ccggatcggc
     1501 accagtgccg gcaacaggcc gttgcaacga ccggagaatc cggcctacat caatatccag
     1561 gctcaactca attctgtgaa ttcttccctg caagccttga aaaccagtcg cgcgaccgtc
     1621 aaacataggc ttcaagatta tgcgcaacgc attgagcgga caccggccct ggaaccggac
     1681 tatttgacgc tcgcgcgcga ccgcgacacc tccgctcaaa agtaccagga cattcgctcc
     1741 cggcttctgg aagcgaaggt gtcggagggc ctcgaagttc agcgaaaggg cgagcgattt
     1801 tctcttatcg atccgccggg actgcccgag tctcccgaga agcctaaccg gaaagcaatt
     1861 gtgctgctgg gattcatcct ggccctggcg ggcggcgcag gggccgccgc gctcacggag
     1921 catctcgacc attcaattcg cacccctgaa caactggtcc gagtgtcgca ggccttcccc
     1981 ctggcggtca ttccttacat gccgaatcgg gcagatctgg ctcgtgcact cgccagaaga
     2041 aacaggattc gtctcgcggg cctgggaacg gtggccctgt tgttgctcat ctgccatctg
     2101 ttttggaccc cgttagatgt ggtgtggtac gcaac
//
LOCUS       DAORQN010000246         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1438_length_2124_cov_4.001450, whole genome shotgun sequence.
ACCESSION   DAORQN010000246 DAORQN010000000
VERSION     DAORQN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1438_length_2124_cov_4.001450"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..357)
                     /locus_tag="PK614_09305"
     CDS             complement(61..357)
                     /locus_tag="PK614_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82441.1"
                     /translation="MSTKIRYRDEPMGEIRVVPDFLPSPAELAFRDEGVKVTLALSRK
                     SVDFFKAEASRHHTQYQRMIRRLLDAYVERQPQDRPSGSPRAARGRAARKRTAA"
     gene            complement(329..619)
                     /locus_tag="PK614_09310"
     CDS             complement(329..619)
                     /locus_tag="PK614_09310"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRB82442.1"
                     /translation="MAKTRFEWDQAKDATNQEKHGVSFQLAQYAFADSRRVISKDVTH
                     SRSEDRFYCFGEVEGGIMTVRFTYRASVIRIIGAGYWRKGKAVYEHENKISR"
     gene            complement(725..1264)
                     /locus_tag="PK614_09315"
     CDS             complement(725..1264)
                     /locus_tag="PK614_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82443.1"
                     /translation="MTMRLNLEQAFPTEQALRDMIVTRKVCFDHDPFYVKTGAGNVVQ
                     IGFQLNLYAAFHDPTQLPLNDDAEHREILGDLQRLCRVFFQTLDLLKPCEHPQPPAHR
                     IVFSPERKNRAEVCLQIPIFDLAHYADSSARQVQDLLATAECLLTHVGARRRVWDEEG
                     RKPESFTGECEARAGAEGG"
     gene            complement(1280..1633)
                     /locus_tag="PK614_09320"
     CDS             complement(1280..1633)
                     /locus_tag="PK614_09320"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRB82444.1"
                     /translation="MSCHSERYFEGRATPAVKTWHRDLVAQRTACTACHTEHRGALAQ
                     ITERTRTSPHGEFVFRATGTSSCTDCHEFGARVATKPTLRDEPIVRRLYEKGGGAHQP
                     GRMAECLACHGRGAS"
     gene            complement(1830..>2124)
                     /locus_tag="PK614_09325"
     CDS             complement(1830..>2124)
                     /locus_tag="PK614_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82445.1"
                     /translation="FLHSGAHYHALVPILAMLSMVIVVVSGIVGQAVHAFSLRALNDQ
                     RRRLQHEGLSDSDIDAQLHGMASQEEAFRMWQAIHAPMTLLHVAGALFFAGIS"
BASE COUNT          457 a          650 c          581 g          436 t
ORIGIN      
        1 ttctagggtt gaagttgctc aacgaatgat cttgggcgat cccccgttgt gattcgtgag
       61 ttacgcagca gtgcgcttgc gagccgctcg tccgcgtgcg gcccgcggtg aacctgaggg
      121 acggtcctga ggctgcctct cgacataggc atcgagcaag cggcgaatca tgcgctggta
      181 ttgggtgtga tgccgggatg cctcagcctt gaagaagtcc acgctcttcc tgctcagggc
      241 caacgttact ttaaccccct catcgcgaaa cgctaattcg gcgggagaag ggagaaagtc
      301 tggaacgaca cgaatctcgc ccatgggctc atcgcgatat cttattttcg tgctcataga
      361 ctgcctttcc ctttcgccaa tagccggcgc cgatgattcg aataaccgaa gctcgatagg
      421 taaaccggac ggtcataatg ccgccctcga cctccccgaa gcagtagaag cggtcctctg
      481 acctgctatg tgtgacatct ttggagatga cacgtcgtga gtcagcaaaa gcatattgag
      541 ctaactgaaa tgacacccca tgcttttcct ggtttgtcgc gtctttggcc tgatcccact
      601 caaaacgtgt cttagccatt gccttccgca gagactacca taaggcaata tttcctgcca
      661 tatgaccata tggcgatttg ggaagatatt ccacctcaat ccggctttgc acgcgtgcgc
      721 aaacctagcc tccttctgca cccgcacggg cttcacactc accggtgaaa gactccggtt
      781 tgcgcccctc ttcatcccac acccgacgtc tcgcaccgac atgcgtcagc agacattccg
      841 ccgtcgcaag aagatcctgc acctggcgag ccgagctgtc ggcataatgc gccagatcga
      901 agatcgggat ctgaaggcaa acttcggcac gattttttcg ctccggggag aagacgatgc
      961 gatgcgccgg aggctgcgga tgctcgcagg gcttcagaag atccagcgtc tgaaagaaca
     1021 cccggcagag ccgttgcagg tcgccaagaa tttcacgatg ctcggcatca tcattgagcg
     1081 gcagctgggt cggatcgtga aacgccgcat agaggttcag ctggaatcca atctggacca
     1141 cattgccggc accggtcttc acatagaacg ggtcgtgatc gaaacagact ttgcgcgtca
     1201 cgatcatatc gcgcagggct tgttcggtgg gaaaggcttg ttcgagattc aatcgcatcg
     1261 tcactccatc atgctccctt caggatgctc ccctgccatg acaggcaagg cactcggcca
     1321 tgcgaccagg ctgatgcgcg ccacctccct tttcatacaa ccgacgcacg atcggctcat
     1381 ctcgcagcgt cggcttggtc gcgaccctcg cgccgaactc gtgacagtcc gtgcaggaac
     1441 tggtgccggt tgcacggaag acaaattcac cgtgagggct agtcctcgtt cgctcagtga
     1501 tttgagcaag cgcgccgcga tgttccgtgt ggcaggccgt gcaagccgtg cgttgcgcca
     1561 ccaggtcacg atgccaggtc ttcacggcag gcgtcgcccg accctcgaaa taccgctccg
     1621 aatggcaact cacacagcga gccggactgg ggccacggaa tggttcgggt acacccggct
     1681 tgatcgacgt ggtatcggct caaatcgccc ggtgcccaga acgaagcggg acttctcacc
     1741 gacccccagc cgatccagac aacaagcaca agcgtcacca gagccaccac cggcaccgca
     1801 gttcttggca gcggatggta tcgcatccgt cacgagattc ctgcgaagaa caacgccccc
     1861 gccacatgca acagtgtcat cggcgcgtgg atcgcttgcc acatgcggaa cgcttcctct
     1921 tgggacgcca tgccatgtaa ctgcgcgtct atatcgctgt cggataatcc ttcgtgctgg
     1981 agtcggcggc gttgatcgtt gagcgctctc aggctgaatg catgcaccgc ctgtccgacg
     2041 atcccgctga cgaccacgat caccatggac agcatcgcga gaatcggcac caacgcgtga
     2101 taatgtgcgc cggagtgcaa aaaa
//
LOCUS       DAORQN010000247         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1448_length_2103_cov_5.012207, whole genome shotgun sequence.
ACCESSION   DAORQN010000247 DAORQN010000000
VERSION     DAORQN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1448_length_2103_cov_5.012207"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1019
                     /locus_tag="PK614_09330"
     CDS             120..1019
                     /locus_tag="PK614_09330"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HRB82446.1"
                     /translation="MGSRGARTYQDNGAKPIEQSTTARQATSPPPSMASGPASTARPT
                     PAAQPSWIQRNPLLAGIAGGLAGSWIGHMLFGATDSSARTNEAGEQVGETGQAGASGP
                     SGMFILLMLMGAGALFFYLRSRKPAVAPDFSGITRSSAVSGSLLADSPVSTLRTATID
                     SETTSADKAAFQQSLTDIQTAWGKQDLSALRRLVTPEMLEYFSTGLAELTSQDQANRV
                     EDVVLLQAEVRESWVENTVQYATVSLHWSARDYTVSLSKAPGDAGYLIEGSAEKPIET
                     TEVWTFMRYQHGKWLLSAMQQVA"
     gene            1734..>2103
                     /locus_tag="PK614_09335"
     CDS             1734..>2103
                     /locus_tag="PK614_09335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB82447.1"
                     /translation="MRIAHISPFLEKVSSESQDPQGRMIFHLTKQLTRLGHEVTVFAS
                     GDSRTAGELIPVAPLSMRSYPAPKRHLAEALAMLALEKAFARLPSFDIIHLHSGLSCL
                     PLMRRSPIPIVSTVYDALDAP"
BASE COUNT          387 a          618 c          657 g          441 t
ORIGIN      
        1 gaagcataca agacagttgg tgagtgttag ccttttgacc ggaatcttcg agatgggcgc
       61 ggggtggagc ggggggaggg tattccggtg gttcgcgcag cggggcctct tccggcagta
      121 tgggcagccg cggcgcacgc acctatcaag acaacggcgc gaagccgatc gaacaatcca
      181 cgactgccag gcaggccacg agcccgccgc cttccatggc cagcggtccg gcctcgacgg
      241 ccaggccgac gcctgctgcg cagccttcgt ggattcagcg caatcctcta ttggcgggta
      301 tcgccggcgg tctggccgga tcatggatcg gtcatatgct gttcggggcg accgacagca
      361 gcgcgcgaac gaacgaagcg ggtgaacagg tcggggaaac cggtcaggcc ggggcttccg
      421 gtccgagcgg aatgtttatt ttgttgatgc tcatgggagc cggggccctg tttttctatc
      481 tgcgatcgcg taagcctgcg gtggcgcctg atttctcagg catcacgcgg agcagcgcgg
      541 tgagcggctc gctgttggca gattcgcccg tgtcgacact acgaacggcc acgatcgata
      601 gcgagaccac ctcagccgac aaggcggcct ttcagcaatc gctgaccgat atccaaaccg
      661 cctggggcaa acaggatctg tccgcattgc gtcgactggt gacgccggag atgctggagt
      721 atttcagtac cggcctggcc gaactgacca gccaggatca ggcgaaccgt gtggaggatg
      781 tggtgctgct ccaggctgaa gttcgggagt cgtgggttga aaataccgtg cagtatgcga
      841 cggtgagcct gcattggagc gcccgggact ataccgtttc cttgtcgaag gcaccgggcg
      901 atgccggtta tctcatcgag ggtagtgcag agaaaccgat cgagacgacc gaagtctgga
      961 cctttatgcg atatcaacac ggcaagtggt tgttgtctgc gatgcagcag gtcgcgtaag
     1021 accctgccga gcgggcccga tggggacagg gtgagctggt tggatacggg ggcggccacg
     1081 cgagtggctg cccccgttct gtttccgctt agaaccctct gaatcatgct tcgctgcgtg
     1141 cggtgatcga gccggtgtcc caggcgccgg acggcacagg ttgtcgaccc gtgcagttcg
     1201 cccgaatgcc acgcagccct tcctccaagg ctcgataagt cactccctgt agcaggatgc
     1261 ggaaaaagtc cgccagcggc gttctcgcat cgcttcgcgg ctcaccgtac cgcacgagta
     1321 cgattcgtcg cttcgctcgc tgcggccttg ctggacggcc tttttgcgca tcctgcgggt
     1381 tatcctgata ccgagacggc gcctgagttg atcgtagcgt attgcgtagc aatcgatttt
     1441 ttccgtggcc tgttagaccc gcttgcttgt gagatggcgc gaccgctcca tgtctccggc
     1501 atcgtgagat cacttcatga tgacttcacc ccctcttcgc cgaattttca tgattgccgt
     1561 tgtaccttct ctttgaaacg tagagcggcg gctgattcgg ccgcctgcac tgctccctca
     1621 aaccatacat tcgtcacggc gaccgttgct gccctgtcga tagctcgctt gcgctgctgg
     1681 acgcgagcct gaagggcacg tctcacgctg gaccggacaa agggaaaaag accatgcgga
     1741 ttgctcacat cagcccgttt ctggaaaagg tgtcatccga gagtcaggac ccgcagggca
     1801 ggatgatctt ccatttgacg aagcaattga cccggctggg acatgaagtg acggtctttg
     1861 ccagtgggga cagcagaaca gccggtgagc tgatccccgt cgctccgctt tccatgcgat
     1921 cctatcctgc gccgaaacgc catctcgcgg aggcattggc catgctggca ttggagaaag
     1981 cgttcgccag gctgccgtcc ttcgacatca tccatctaca ctcaggattg tcctgtttgc
     2041 cgctgatgcg tcgtagcccg atcccgatcg tctcgaccgt gtacgatgct ctggacgccc
     2101 ccg
//
LOCUS       DAORQN010000248         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1454_length_2095_cov_3.880392, whole genome shotgun sequence.
ACCESSION   DAORQN010000248 DAORQN010000000
VERSION     DAORQN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1454_length_2095_cov_3.880392"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK614_09340"
     CDS             complement(<1..284)
                     /locus_tag="PK614_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRB82448.1"
                     /translation="MITLRYTPWLVAFLLTVTGCNSEQASSGGQPPPPEVVVAQVLSK
                     SVQQWDEYTGRITAIDTVELRPRASGYVQRVAYKEGQDVKQGDLMFQIDPR"
     gene            519..1007
                     /locus_tag="PK614_09345"
     CDS             519..1007
                     /locus_tag="PK614_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRB82449.1"
                     /translation="MKRIKKMQAPRAKGKSAVSPCISKLPRHRMVELLWSFAPAYQRW
                     SESLLVEKGLSPQRLRILGSIHERPRIMSELKKELGVTATNVTALVDSLEKDGLVVRT
                     RHPTDRRATVIELSDKVMTELSPRCTEYKEQVAELFSDLSDQECKAFVKTLEKLWSRL
                     QG"
     gene            complement(1176..>2095)
                     /locus_tag="PK614_09350"
     CDS             complement(1176..>2095)
                     /locus_tag="PK614_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HRB82450.1"
                     /translation="RPVSHPDLPCKQLAAHSRQSVFQGRVHLGGALSGRDTTAVQKTV
                     SGLVKLLSPNPESPVTDELLEWASRLALECRRRVKEQQKRIGSAEFRNTQFSYSLGEE
                     GVEQFVATPELYSEDSIGTDPLPPGQVWVISPNAQEEHSGLYRIDATEGPGSGVKILN
                     QPAPAPFKESVRCAEQNLYTRAKELVGDRAPREHEFSVQLRAFDASKSGASVGVGVLL
                     ALCSALLQKSLKGGLVVAGGLNLGGSIEPIYNAVNVAELAVEKGATTLLIPVSARKQM
                     NDLSDDMATKINIQYFTEGKDALIKALAD"
BASE COUNT          463 a          578 c          570 g          484 t
ORIGIN      
        1 cgagggtcga tctggaacat caaatcgccc tgtttcacat cctgaccttc cttgtaagca
       61 actcgctgca catagccgct ggcgcgcggt cgcaattcga ctgtgtcgat cgcggtgatg
      121 cgaccggtgt attcatccca ttgttgcacc gacttcgaga gcacctgtgc gaccacgact
      181 tcaggtggag gcggttgtcc gccgctgctg gcctgctcgc tgttgcagcc tgtcacggtc
      241 agcaggaatg cgaccagcca tggtgtatat cggagagtga tcatggtgta cctactggga
      301 atactctttg tgaacgagag ccctagatca aggctgtcgt cgatgttttt cgaattgatg
      361 gtctcagtct cacagcgcct gtggctgaat gtctgtcctc atgactccca tgcagcgcag
      421 tatccggcat ggcaggaaac atagtcaata tatgaatgat caggttatga acaatttatc
      481 attgaatgtt gtctttgttt gaagtaccgt gacacacgat gaagcgtatc aaaaaaatgc
      541 aagcccccag ggccaagggg aagtcagccg tctctccctg catctcgaag ttgccacggc
      601 accggatggt cgaactgttg tggagctttg ccccggccta tcaacggtgg tcggaatctc
      661 tattggtgga gaaggggttg tcccctcaac ggctgcgcat tcttggttcg attcatgagc
      721 gcccgaggat tatgagcgag ctcaagaagg aactaggcgt gacggcaacg aacgtgacgg
      781 cgctcgtcga ttccctcgag aaagacggat tggtcgtgcg aaccagacac ccgacggatc
      841 gccgggccac cgtgattgaa ctctcggaca aagtcatgac cgagctctcc ccgcgctgta
      901 cggaatacaa agagcaggtc gccgaactgt tctccgatct gagcgaccag gagtgtaaag
      961 cgttcgtgaa aacgctcgag aagctctgga gtcgattgca ggggtaaaga gaggcgagtg
     1021 gcgtcagaac ctcttggctt ccgtacctta acggcgatga ccctatcgac ggccaactca
     1081 cggtgcggag tcacttcaga gccgagtaaa tcgatctagc tctgcctgac atagcatcgg
     1141 gaacacacat ctgagatgcg tgattgccat tgatcttagt ctgcgagcgc tttgatgagc
     1201 gcgtcctttc cctcagtgaa atattgaatg ttgatcttgg tggccatgtc atcggaaaga
     1261 tcgttcattt gttttctcgc agagacaggg atcagaagag tggttgctcc cttctccaca
     1321 gcgagctccg cgacgtttac cgcattgtaa atcggttcaa tcgaaccgcc gagattcagc
     1381 ccacccgcga caacaagtcc acccttcagg cttttctgca agagggcgga acagagggct
     1441 agcaacactc cgaccccaac cgacgcacca ctcttcgatg cgtcaaatgc ccgaagctga
     1501 actgagaact catgttctcg gggagctcga tctcccacca actctttggc ccttgtgtag
     1561 agattctgct cggcgcatcg aacgctttcc ttgaacggag cgggagccgg ctgattcaat
     1621 atcttgacgc cactgcctgg accctcagtc gcatcaattc gatagaggcc tgaatgttcc
     1681 tcctgcgcgt tgggcgaaat cacccagact tgcccgggag ggagcggatc agtgccgatg
     1741 ctgtcttcgc tgtagagttc cggagtagct acgaattgtt ccacaccctc ttcgcctaac
     1801 gagtaactga attgggtgtt ccgaaactcg gccgatccga tgcgcttctg ctgttccttc
     1861 acgcgtcgcc tgcattccaa cgcaagccgc gaggcccatt ccaacagttc atctgtcaca
     1921 ggggattctg gattcggtga cagcagcttg accagcccgc tcactgtctt ctgcaccgct
     1981 gttgtatcac ggccgctcaa ggctccgccg agatgaactc ttccctggaa caccgactga
     2041 cggctatgtg cagcgagttg cttacaagga aggtcaggat gtgaaacagg gcgat
//
LOCUS       DAORQN010000249         2081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1467_length_2081_cov_5.438796, whole genome shotgun sequence.
ACCESSION   DAORQN010000249 DAORQN010000000
VERSION     DAORQN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_2081_cov_5.438796"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK614_09355"
     CDS             <1..403
                     /locus_tag="PK614_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82451.1"
                     /translation="DTVVMAGSVHKILTMYRKNRARFLYVPNALLSGDSDCTVLLTFD
                     DIARRLTTEKVIHVPKCIMQSGRGPYTDITGVTLEQFSKKTGVRVKVLHKIDTRFANQ
                     QLYRNGSLQNYVEQYLKHPLRGAYDAMPRPV"
     gene            1023..1952
                     /locus_tag="PK614_09360"
     CDS             1023..1952
                     /locus_tag="PK614_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRB82452.1"
                     /translation="MPGPRRLQFYQADVFTDQPFGGNPVAVFPDADGLTDVELQQIAR
                     EMNLSETVFVFPPTDKAAVVKMRIFTPTQEIPFAGHPVIGTFFVLATLDRLALREPVT
                     RVLQECNLGLFPVDIHTCNGVIERVVMAQPSPQFLDVIDEPEALFAIARALGITKAVI
                     SETRFPVQVVSTGLPVIIVPVRTLTAVRQIIPDVAAIAELSQQYGANGIMVFSTMTVE
                     QSSSVHTRMFAPLIGIVEDPATGSASGALGAYLVQHGVVDIRPSTEITAEQGYEIDRP
                     SRILIQVDSDDDVIQGVTVGGQAMMGVEGTLTF"
BASE COUNT          383 a          627 c          615 g          456 t
ORIGIN      
        1 agacacggtc gtgatggcgg gcagcgtcca caaaatcctg actatgtacc ggaagaaccg
       61 cgcgcggttt ctctatgtgc ctaatgcgtt gttgagcgga gactccgatt gtacggtgct
      121 cctgaccttc gacgacatcg cgcggcgact gaccacggag aaggtgatcc atgtgcccaa
      181 gtgcatcatg cagtcggggc gggggcccta taccgacatc accggcgtga cgctggagca
      241 gttcagcaag aagaccggtg tgcgggtgaa ggtgttgcac aagattgata cccgcttcgc
      301 caaccagcag ctctaccgca acggatcgct tcagaactat gttgagcaat acctcaagca
      361 tccgttgagg ggtgcgtatg atgccatgcc acgcccagtc tagcagggtg ttgaaaacga
      421 cctcccacgt cgttctcggc tcgccaacat cctcaacgta cccctgaggg tacgcctccg
      481 gtgttggctc acctgcggcc tagtgggacg tcgttttgaa caccctgcgg gaggcggatc
      541 ggatcggccg ataaaacgcc atcatcctta cccgtcctcc ccgacaggcc gagacatgcc
      601 tggatacggc ggcgttctcg gtcgcctgtc tccctgcgac gtacccgtaa gggtacgccc
      661 cagtcgctac gctccctgcg gccttggtat atgccgtttc gaccagccgg cgggatgcgg
      721 attagaccaa atgctggcac gtgccaatgc cacagcgtcg ttctcggtcg cccttctcct
      781 cgcggactgg taggtgccgt tttggagtag ctcgcgggag cggcacgtat cttgtgaatc
      841 gttagtcgtg aagcgatagc cctcacggtc aaccgacgat cgttcgacac ctgttcaccc
      901 ttcaattttg aatcctcaat tggggagcat gattcttttc cttttctgcg acagtgctgc
      961 gccctcttgc aaaaaatacc tgctcggggc aacatgccgc caacatcttt cgtgaggaat
     1021 ctatgcccgg tcccaggcga ctgcagttct atcaagccga cgtgttcacg gatcagccgt
     1081 tcggcgggaa tcccgtcgcc gtgtttcccg atgccgacgg cctgaccgac gtggaattac
     1141 agcagatcgc ccgcgagatg aatctttccg aaaccgtgtt cgtgtttccc ccgaccgaca
     1201 aggccgccgt cgtgaagatg cgcatcttta ccccgacgca ggaaattccc tttgccgggc
     1261 atccggtgat cgggacgttt tttgtgctgg ccaccttgga ccgtctcgcg cttcgggagc
     1321 cggtcacgcg ggtgctccaa gaatgtaatc tcggcctctt ccctgtggac atccatacct
     1381 gcaatggcgt gatagagcgc gtggtcatgg cgcagccgag cccgcagttt ctggatgtga
     1441 tcgacgagcc ggaagcgctc ttcgcgattg cccgcgcctt gggcattacc aaggctgtga
     1501 tttccgagac acgtttcccg gtgcaggttg tttctacggg gttgccggtc atcatcgtgc
     1561 cggtccggac gcttacggcg gtgcggcaga tcattcccga cgtcgcggcc atcgcggaac
     1621 tgtcccagca gtatggcgcc aatggcatca tggtgttcag cacgatgacc gtcgaacagt
     1681 cctccagcgt gcatacgcgg atgttcgccc cattgatcgg catcgtggaa gatcccgcga
     1741 ccggaagtgc gagcggggcg ttgggtgcct atctggtcca gcatggcgta gtggacatcc
     1801 ggccatcgac cgagattact gccgagcagg ggtatgagat cgatcgcccc tcccgcatcc
     1861 tgatccaggt ggactcggac gacgatgtga ttcaaggggt gacggtcggc ggacaggcca
     1921 tgatgggggt ggaggggacg ctcacctttt agccggatca tgaaaacgcc atctcactgc
     1981 gttctcagtc gcacgtctcc ttgcgacgta cccgttcggg tacgccgcag tcaccgcact
     2041 ccctgcggcc ttgttagatg gcgttttgat catcctgtgg g
//
LOCUS       DAORQN010000250         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1470_length_2076_cov_10.646710, whole genome shotgun sequence.
ACCESSION   DAORQN010000250 DAORQN010000000
VERSION     DAORQN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_2076_cov_10.646710"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..685)
                     /gene="ybeY"
                     /locus_tag="PK614_09365"
     CDS             complement(215..685)
                     /gene="ybeY"
                     /locus_tag="PK614_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRB82453.1"
                     /translation="MPVVIGMRLTRWPLRLGALKQLAMHVLQAVGEAETLLSLEIVGD
                     VRMRRLNRTFRHRDKTTDVLAFATREGPGPPSSLLGDVVISLPQAIRQARRHEQGVDH
                     ELAVLLIHGILHLCGYDHERSEAEAQRMSRREKAVLRAVAPVPRLLVSRGSDRV"
     gene            complement(685..1746)
                     /locus_tag="PK614_09370"
     CDS             complement(685..1746)
                     /locus_tag="PK614_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRB82454.1"
                     /translation="MRKIKLREGTNTAALFGHHDRHLKLIEEEFGVRCSARGEEVTVE
                     GTPETVKQAERVLNELASLTNEGYDLRSDDVTHALTALRHNHDASLKELLFSASPIVT
                     RKRFIVPKTPTQKYYLDAIEKHDIVIGIGPAGTGKTYLAMAMAVSALMKKDVSRIILA
                     RPAVEAGEKLGYLPGDMYAKVNPYLRPLYDALFDMMDMERANRLIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFHSKAVVTGDITQIDLPSDRVSGLIEV
                     RDILQDIAGIEFVYFDERDVVRHRLVQEIIKAYDRHTASPAPPAQPRKGEAQTKGHRS
                     DSKPTRPAAPSTGSWGQSH"
     gene            complement(1862..>2076)
                     /locus_tag="PK614_09375"
     CDS             complement(1862..>2076)
                     /locus_tag="PK614_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRB82455.1"
                     /translation="SISYGLETGPPGMTIDKATGHVTWKVTPGVGGTHRIKIMVEDGQ
                     GGTAWQDFELSIPSTAQSLTVPPTQG"
BASE COUNT          439 a          642 c          582 g          413 t
ORIGIN      
        1 caaggccacc tccagctcat ccagcagcgc cggatcagcc gcccgcccca ggagtcgatc
       61 cagttgtccc gtgactgcat cacgtgtttt ggagagccca gcacttagct tctgaagcca
      121 acccaccgcg tacctcaccc tctcttcgac cgacccgaat gattgatgaa cgatctagcc
      181 taggacttct cgtgcacggc gatcaaatgc cgcatcatac ccgatccgat ccgcgtgaca
      241 ccaatagccg tggaaccgga gcaacggcgc gaagcacggc cttttcgcgc cggctcatgc
      301 gttgcgcctc cgcctcactg cgctcgtggt catacccgca caaatggaga atgccgtgaa
      361 tcaggagcac cgctaactcg tggtcaacgc cctgctcatg acgacgcgcc tgacgaatgg
      421 cctgcggcag ggaaataacg acgtccccga gcagagacga gggtggcccg ggcccttccc
      481 tggtcgcaaa cgccagcaca tccgtcgtct tgtcgcgatg acgaaaggtt cgattcaggc
      541 gccgcatcct gacatcgccg acgatctcaa gactcaacag cgtttcggct tcgccgactg
      601 cttgcaagac atgcatggcg agttgcttga gggcgccgag gcgaaggggc caccgcgtca
      661 gccgcatgcc gatcacaacc ggcattaatg agactggccc caggaacctg tggaaggagc
      721 cgcgggacgg gtgggcttag aatccgaacg atggcccttc gtctgagcct cgcctttccg
      781 tggctgagcc ggaggcgcag gactggccgt atgacgatcg taggccttga tgatctcctg
      841 gacaaggcga tgccgtacga catctcgctc atcgaagtag acgaactcga tcccggcaat
      901 gtcctgaaga atgtcgcgca cctcgatgag gccggacacc cggtccgacg gcaggtcgat
      961 ctgcgtaatg tcccccgtca cgacggcctt cgaatggaat cctaaccgtg tgagaaacat
     1021 cttcatctgt tcggcagtcg cgttttgcgc ttcgtccagg atcacaaatg aatcgttcag
     1081 ggtacggccg cgcatgaacg caaggggcgc aatttcgata tcccctcgct caatcagacg
     1141 attggcccgt tccatatcca tcatatcgaa cagggcatcg tagagcggcc gaaggtaggg
     1201 attgaccttg gcatacatat cgccgggcaa ataccccagc ttctcgccgg cctcaacagc
     1261 cggccgcgcg aggatgatgc ggctgacatc ttttttcatc agcgcactga ccgccatcgc
     1321 catggccaag taggtcttgc ccgtgcccgc aggaccgata ccgatcacga tgtcgtgttt
     1381 ttcgatcgcg tccagatagt atttctgagt cggagtcttc ggaacgatga atcgcttccg
     1441 agtgacgatg ggggaagcgc tgaaaagcag ttctttgaga gaagcgtcgt ggttgtggcg
     1501 gagagcggtc agcgcatgcg tcacatcatc ggaccgcagg tcataccctt cattcgtcaa
     1561 cgaagcgagt tcattgagaa cccgttccgc ctgcttgacg gtctcaggcg taccctccac
     1621 cgtcacttct tcaccccgcg ccgagcatcg cacaccgaac tcttcctcaa tcagcttgag
     1681 gtgtcgatca tggtgcccga acagggctgc ggtattcgtg ccttcccgta gtttgatctt
     1741 gcgcacgccg gtgtcgaaaa ctcctttcgt caggacgata gggagattct aacaaaccgg
     1801 gaaagtccgg aacaaggtcg gaggggacag aacagaaaat aaggaggccc accggaaccg
     1861 actagccttg agtgggggga accgtcagcg attgggcagt ggagggtatc gagagttcaa
     1921 aatcctgcca ggccgtgcca ccctgcccat cttccaccat gatcttgatg cgatgagttc
     1981 ccccgacacc cggcgtcact ttccaggtca cgtggcccgt cgccttgtcg atcgtcatcc
     2041 ctggaggccc tgtctcgagc ccatagctga tgctat
//
LOCUS       DAORQN010000251         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1480_length_2064_cov_18.684420, whole genome shotgun sequence.
ACCESSION   DAORQN010000251 DAORQN010000000
VERSION     DAORQN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_2064_cov_18.684420"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..619)
                     /locus_tag="PK614_09380"
     CDS             complement(278..619)
                     /locus_tag="PK614_09380"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRB82456.1"
                     /translation="MPDRLTILLDQNVPQAVVSWLKVLKPTWKVFHTGDVGLSGKADE
                     EVFAWAQQQGAVVVTFDEDFADGRSFPAQRHHGIVRLRVWPTTIEETEQALGRLLSEV
                     TEANSPRLSSS"
     gene            complement(612..833)
                     /locus_tag="PK614_09385"
     CDS             complement(612..833)
                     /locus_tag="PK614_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRB82457.1"
                     /translation="MDDRIVVDPNICSGKPTIRGTRIMVKNILGMVAGGYTMARIIDA
                     YPELTNNDVTAALEYASQVIDEEKVIPRA"
     gene            1185..1859
                     /locus_tag="PK614_09390"
     CDS             1185..1859
                     /locus_tag="PK614_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82458.1"
                     /translation="MNEARARGRAKGTIRKKCLTIGADHLVTLTYRANVEDRERVLHD
                     LERLRRALSRSGCSMPYVAVLERQQRGALHPHLAVKGFQDVRLLRRCWYKIVGNGQGQ
                     VNVRGPRPGSSPVKLARYLSKYISKDFDNMPREFEEHRYFCSLGVKVPTEKHEFVLDR
                     RAKDVEMKMYSILRQEALRRVGVCCRLTEWMGGSGTYGWMAGFEDSSIRWGTGAPEPV
                     PISPTS"
BASE COUNT          459 a          555 c          609 g          441 t
ORIGIN      
        1 cttgataaat ggagccggcg agtggaatcg aaccaccgac ctgcggttta cgaagcgatc
       61 ccgaagtaat ttccccacct cattctacac cacaaaaaca cacagaagag cctgatccac
      121 cattgggtta ggtggatcgt gcttcgtcat gtggtgtggt gtcaaaaacc gccggaaacg
      181 gtctcgacgg ttacactttt ggttacactg cacgcctcgc tttttgcggg gcgtgcaacc
      241 tattgaggtc gggtccgcac gcggattcga gagcggtcta cgatgatgag agacgtggtg
      301 agttcgcctc ggttacctca ctgagcaatc gacccaaagc ctgttcggtt tcttcgatcg
      361 ttgtcggcca aacccgaagg cgcacaatgc catggtgtcg ctgtgctgga aaggaacggc
      421 catcggcgaa gtcttcatca aaggtcacaa cgacagcgcc ctgttgctgc gcccaggcaa
      481 agacctcttc atcagccttc ccggacaatc ccacatcgcc ggtgtggaaa accttccacg
      541 tcggcttcaa caccttcagc catgaaacga cggcttgggg aacattttgg tcgagaagga
      601 tggtcaggcg gtcaggcacg cggaatgact ttctcttcgt caatcacctg gctggcatac
      661 tcaagcgcgg cagtgacatc gttgttggtc agttcggggt atgcgtcaat gatcctggcc
      721 atggtgtagc caccggccac catgccgagg atgttcttca ccataatgcg ggtgcctcga
      781 atcgtgggct tcccgctgca tatgtttgga tcgacaacga tacgatcatc catgatgagc
      841 tcctcactga ctaaccggta acttactgta aggctaggag gttccacgtg tcctgtcaag
      901 atatgccgct cgactcaaac agcagcaacc aaaagccgga cgcccgtgcg cggggtggcg
      961 caagccaccc gcgcagggga gccgcgccag cggccactcc taaacttgac aggaggacat
     1021 ttctcggcga ccacacctgg cctcacatca cactacctcc cgcagggaat gtctggtccg
     1081 tcacgattca tgatttcgga aaacaatggg tcgaaatgtc ttgggggagg ctagacccaa
     1141 ttcaaggcaa gaaaggagag aaaggcgcat cgcggaatag aaagatgaac gaggctcggg
     1201 cacgaggtcg ggccaaaggc acgattcgca aaaaatgcct gaccatcgga gccgaccacc
     1261 tcgtgacttt gacctatcga gcgaatgtgg aggatcggga acgtgtgtta cacgatctgg
     1321 aacggttacg gcgggcgctc tcacggtccg gatgctctat gccctatgtg gcggtcctgg
     1381 aacgtcaaca gcgtggggcg ttgcatccgc atctcgcggt gaaaggcttt caggatgtac
     1441 ggctcttgcg gcgatgctgg tacaagatcg tcgggaacgg acaaggacaa gttaatgtcc
     1501 gagggcctcg tcccggcagt tcgcccgtaa agcttgcacg ctatctctcg aagtacatca
     1561 gtaaagattt cgacaacatg ccgcgtgagt ttgaggaaca ccgatacttt tgttctttag
     1621 gggtcaaggt gcctacggaa aagcatgagt ttgtgctgga ccgaagggca aaggacgttg
     1681 agatgaaaat gtacagcatc ctgcggcagg aggctctgcg tcgcgtcggc gtttgctgcc
     1741 gtctcacgga atggatgggt ggatcgggca cctatggttg gatggctggg tttgaggata
     1801 gctcgatacg gtggggtacc ggcgcgcctg aaccggtgcc gattagtccg acctcgtgac
     1861 ccactgcgcc ggcgcttgcg gaagcgcggg cggtctcggg aaagggggtg tcgaagactc
     1921 ccccgctttc tagtacgagc tcattagcaa tttcagtcac ggtcaaacca ggctgttcta
     1981 gccggacctc ggatatccct tgtggaatga gctggcgcga tgggctagtg tcgctcccag
     2041 tattctcact ctaggtttcc gcgt
//
LOCUS       DAORQN010000252         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1484_length_2062_cov_11.097160, whole genome shotgun sequence.
ACCESSION   DAORQN010000252 DAORQN010000000
VERSION     DAORQN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1484_length_2062_cov_11.097160"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK614_09395"
     CDS             complement(<1..217)
                     /locus_tag="PK614_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB82459.1"
                     /translation="MSNTAVSVTGLSKRYRLGTTHAQDGSLAGALTRGLRRLIGGQPA
                     SPQTHDTLWALRDVSFEIKKGEVFGVIG"
     gene            complement(283..519)
                     /locus_tag="PK614_09400"
     CDS             complement(283..519)
                     /locus_tag="PK614_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB82460.1"
                     /translation="MELHDRLEEVFRQVFDNESLTLTNEMTAPDIEGWDSVAHINLMF
                     GIEQAFGVRFKGNELAEMKNIGELKQFLIGKGAS"
     gene            complement(671..>2062)
                     /locus_tag="PK614_09405"
     CDS             complement(671..>2062)
                     /locus_tag="PK614_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB82461.1"
                     /translation="LGALAALCHLPDRHNWLLQRLSVEFRNPLAVGIPYRLEALETSA
                     ERACVKLYDATRLMLKATFTFVPGCEKRRDTHVPEAACLTEAEDRKKEDLPAGTRVTG
                     VYGPATSELVQVITRWKLSEKGTTAMQITAMLWASFVVGMELPGKRAVFWRLELTLYP
                     EDGPQDTLLSYDVTVQDFDERFDLLHSAGTLSAAGTRCATADMWAFVRQDSPQPSLRR
                     LTDLIPRSDRLKGKVALVIGGSRGLGAAITQALASQGCTVFLNYHQCRAEAEKIRASL
                     GDTSNLIELAQGDASEIEWCRTLRQRIVERYGGLDLLIGNASPPIRPLSFVPEKLAQF
                     QNFLTQSVSLASMPMSTFLNDLSERSGWNVLVSSAFVKDRPAEWPHYVTAKCAIEGLV
                     QWAAVHSPKVHTLIVRPPKLLTDQTNTTVGRQGAMEVERAAAAIVTRITCPDSSPTVQ
                     ILETFELNPEASQ"
BASE COUNT          414 a          614 c          591 g          443 t
ORIGIN      
        1 tgccgatgac tccgaagact tcgccttttt tgatttcaaa cgacacgtcg cgcaacgccc
       61 atagggtgtc atgggtctgg ggggacgccg gctgcccacc gatgaggcgg cggagcccac
      121 gcgtcaaggc tccggccagg gagccgtctt gagcatgcgt cgtccccagg cgatagcgct
      181 tggagagacc ggtaacgctg acagcggtgt tgctcatcta aatcagatcg gcgaaggttc
      241 ctgttgcttc gttcgttgat cgacccgtgg ctgcggtatg ggttagctgg ctcccttgcc
      301 gatcaggaat tgcttgagtt cgccgatatt cttcatttcg gccaactcat tgcccttgaa
      361 ccgaactccg aacgcctgtt cgatgccgaa catcagattg atatgggcca cggagtccca
      421 gccttcgata tcgggtgccg tcatctcatt ggtcagcgtg aggctctcat tgtcgaacac
      481 ttggcgaaac acttcttcaa gtcgatcatg cagctccatt cattccccca actctcagtg
      541 ctgtaagtac ctgtccatca tcatgtcgtg gccgggaatc catcttcccg tcacccctgc
      601 ttctgtaatg ccgcttcatg cagctgcggc acgaggtcaa tcggcttcgt cgcacaatgg
      661 ccccggaggt tcattgcgaa gcctcaggat ttagctcaaa cgtctcgagt atctgtacgg
      721 tgggtgatga gtcggggcaa gtgatccgtg tgacgatcgc agcggcggcg cgttcgacct
      781 ccattgctcc ctgccgtccc accgtggtgt tggtttgatc cgtcaacaac ttcggtggtc
      841 taacgataag ggtatggacc ttcggagagt gaactgcggc ccactgcacc aggccctcga
      901 tcgcacattt cgcagtgaca tagtggggcc attccgcagg acgatctttc acaaacgccg
      961 aagagacaag cacgttccag ccggagcgtt cggacaagtc gttgagaaac gtggacatgg
     1021 gcatactggc cagcgagaca ctctgcgtga ggaagttctg aaactgcgcg agcttctcag
     1081 gcacgaacga cagcggacga atgggcggcg atgcattgcc tatcagcaga tcaagtccgc
     1141 cgtatcgctc gacaattctc tgccgtaacg ttcggcacca ttcgatctcc gacgcatccc
     1201 cctgagccaa ctcaatgagg ttggaggtgt ctccaaggct cgcccgaatc ttttccgcct
     1261 ccgccctgca ttgatggtag ttcagaaaca ccgtgcagcc ctgcgaagcc agcgcctgag
     1321 tgattgccgc accgagtcca cggcttccac cgatgactaa cgcgaccttc cccttcagcc
     1381 ggtctgatct cggtatcaga tcggtaagcc gacgaaggga gggctgcgga gaatcctgtc
     1441 gtacaaaagc ccacatgtcg gccgtcgcac aacgggtgcc agctgccgag agagtgcctg
     1501 cactgtgcaa gagatcaaat cgttcgtcga agtcttgaac cgtcacatca taggaaagca
     1561 gcgtgtcctg tggtccgtct tcaggataca aggtgagttc caaccgccag aagacggcgc
     1621 gcttgccggg aagctccatc ccgacgacaa agctggccca cagcatcgcc gtgatctgca
     1681 tcgcggtggt gcccttttcg gagagtttcc agcgagtgat cacctgtacc agttcgctgg
     1741 ttgccggccc gtatacgccg gtcacgcggg tgcctgccgg aagatcctcc ttcttccgat
     1801 cctctgcttc cgtgagacag gccgcttcag gaacgtgcgt gtcacgcctc ttctcacacc
     1861 caggaacaaa tgtgaacgtc gccttcagca tcaggcgcgt ggcgtcatag agttttacgc
     1921 acgcccgttc tgccgaggtt tcgagcgcct ccaggcgata gggtatgccg acggcgagag
     1981 gattgcgaaa ctcgaccgag aggcgttgca ggagccagtt atggcggtcg gggagatggc
     2041 agagggccgc cagcgcgcca ag
//
LOCUS       DAORQN010000253         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1487_length_2061_cov_5.203390, whole genome shotgun sequence.
ACCESSION   DAORQN010000253 DAORQN010000000
VERSION     DAORQN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_2061_cov_5.203390"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK614_09410"
     CDS             complement(<1..381)
                     /locus_tag="PK614_09410"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRB82462.1"
                     /translation="MFNVILYQPEIPPNTGNIIRLCANTGASLHLVKPLGFTLEDKQL
                     LRAGLDYHEFASITVHEGWAECLACMEGRRIFAVSTKGRQRYDLVRYIEGDAFVFGPE
                     SRGLPAEILGSVPEQQCLRVPMVPG"
     gene            complement(510..965)
                     /locus_tag="PK614_09415"
     CDS             complement(510..965)
                     /locus_tag="PK614_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRB82463.1"
                     /translation="MNEQDTQRAVAILNRIMEHELAGVVRYTHYALMVYGYNRIPIVS
                     WLKGNADESLAHAHKAGELVTLLGGHPSLKIGTLLETEKHDVGDILRESLEHEKAAVA
                     SYYELLKLSEGKSVLLEEYAREMIVGEELHLDEVNKMLRKSGDVQAFKS"
     gene            complement(1023..>2061)
                     /gene="ychF"
                     /locus_tag="PK614_09420"
     CDS             complement(1023..>2061)
                     /gene="ychF"
                     /locus_tag="PK614_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRB82464.1"
                     /translation="FNALTKSGIAAENYPFCTIEPNIGIVEVPDARMQALADIVKPQR
                     MQYATTEFVDIAGLVAGASKGEGLGNQFLANIRETDGIVNVVRCFEDDNVVHVAGKVD
                     PISDIATIVTELALADLTTVEKAQERNVKLVRSGDKDAAKLGELLVQVAACLNEGKPA
                     RTLKLDPAQRALLKPLCLLTMKPVMYVANVAEKGFTNNPLLTRVEEYAAHEGAPVVAI
                     CAALESEIAVLSDEEKREFLADIGMTEPGLNRLIRAAYQLLGLQTYFTAGVKEVRAWT
                     IHIGDTAPQAAGVIHGDFEKGFIRAEVIGYNDFIACKGEAGAKEKGKMRLEGKEYVVQ
                     DGDVMHFRFNV"
BASE COUNT          411 a          661 c          539 g          450 t
ORIGIN      
        1 tcccggcacc atcggtacgc gcaggcactg ctgttcaggg acgctgccaa gaatctccgc
       61 cggcaatcca cggctttccg ggccaaagac aaacgcatcg ccttcgatgt aacgaaccag
      121 atcgtaccgc tgtctgccct tcgtggatac ggcgaaaatt cggcgccctt ccatgcatgc
      181 taggcactcg gcccacccct cgtgcaccgt gatcgaggcg aattcgtgat aatccagtcc
      241 ggctcgcagc aactgtttgt cttccagcgt aaagcccaac ggcttgacca ggtgcaacga
      301 ggcgccggtg ttggcgcaga gacggatgat gttccccgtg ttcggaggaa tttccggctg
      361 gtacagaatc acgttaaaca tgaaggcagg gtcacgggtg acggtgagtg gccggcttca
      421 atgcgcggca gcaatcggcg tagtggatca tgacgaaaac ctggtccgga actccggacc
      481 aggcctgtgc cgtcaggcag cgtgaatagt taagacttga aggcctgcac atcgcccgac
      541 ttgcggagca tcttattcac ttcgtccaga tgcagttctt ccccgacgat catctcgcgg
      601 gcatactctt ccaacaacac ggacttgccc tcactcagtt tcagcaattc gtaatatgag
      661 gcaacggcgg ctttctcgtg ctccagactc tcccgcagaa tatcccccac atcatgcttc
      721 tccgtttcga gcagggtacc gatcttcaac gaaggatggc cgcccaatag agtcaccagc
      781 tcaccggctt tgtgggcatg ggccagactc tcatccgcat tgcccttcag ccaggacaca
      841 atcgggatac ggttgtaccc gtagaccatc agcgcgtagt gcgtgtaccg aactacgccg
      901 gccaactcat gttccataat tctgttgaga atcgctacgg ctcgctgtgt gtcttgttcg
      961 ttcacgttgg atctccgatc aggcaagcaa accctgcctc gtaacatggt aaaagagcgc
     1021 cgctagacgt tgaaccggaa gtgcatcacg tcgccgtcct gcaccacgta ctccttccct
     1081 tccagccgca tcttcccttt ttccttcgcc cccgcttcgc ctttgcaggc gatgaagtcg
     1141 ttgtagccga tcacttccgc gcgaatgaac cccttctcga aatcgccgtg aatgacgccg
     1201 gcggcctgcg gcgccgtatc gccgatgtga atcgtccagg cgcggacttc tttcacgccg
     1261 gcggtaaaat acgtctgtaa gcccaggagt tggtatgccg cgcggatcag tcgattcagc
     1321 cccggttcgg tcatgccgat gtcggccagg aattcccgct tctcttcgtc tgagagcacc
     1381 gcgatttcgg actccagtgc ggcgcagatg gcgacgaccg gcgccccctc gtgcgcagca
     1441 tattcttcca ctcgcgtcag cagcggattg ttggtaaacc ctttttcagc cacattggcc
     1501 acatacatca caggcttcat cgtaagcaga cacaagggct tcaacaaggc gcgctgcgcc
     1561 gggtcgagtt tcaaggtgcg agccggcttc ccctcattca aacaggcggc cacctgcacc
     1621 agcaactcgc ctagctttgc ggcatcttta tcgcccgagc gaacgagctt cacgttccgc
     1681 tcctgagctt tttccactgt ggtgaggtcg gccagcgcga gttccgtcac aatggtcgcg
     1741 atgtcggaaa tcggatcgac cttccccgcc acgtggacga cgttgtcatc ttcaaaacag
     1801 cgcacgacat tcacaatccc gtcggtctcg cgaatattgg ccaaaaactg gttgcccagg
     1861 ccttcccctt tcgaggctcc ggccactaac cctgcgatgt ccacgaattc ggtcgtcgca
     1921 tactgcatcc gctgcggctt cacgatgtcc gccagcgcct gcatccgcgc atccggcacc
     1981 tccacgatgc cgatattcgg ctcgatcgtg cagaacggat agttctcggc tgcgatcccg
     2041 gacttggtca gcgcattgaa c
//
LOCUS       DAORQN010000254         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1488_length_2059_cov_6.990519, whole genome shotgun sequence.
ACCESSION   DAORQN010000254 DAORQN010000000
VERSION     DAORQN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1488_length_2059_cov_6.990519"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK614_09425"
     CDS             complement(<1..353)
                     /locus_tag="PK614_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82465.1"
                     /translation="MALATGPSIAAETGAPSTALTQALSQISSERMLADIRALSEPAF
                     NGRQTGTPDDLASAEFVRQRFLELQQHRSPGSESPAPPDRLPAHTQIQSAPVRITQID
                     DDSLLQIGGIAESQPS"
     gene            complement(461..898)
                     /locus_tag="PK614_09430"
     CDS             complement(461..898)
                     /locus_tag="PK614_09430"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB82466.1"
                     /translation="MDLSQQPPRRWSDTLAGMMWLPRLIDKVRAFQAGTLGTYAYPSA
                     LDQSFMRHLRFTPAHIEPLVREMTSDEAIGAAIRQHIPLSDEEIRTRCAAFQEKYWWA
                     FAVLDRDDGYVRGLGYPIPRFLQPPLWRWYQRWSAQKASATSI"
     gene            1098..1343
                     /locus_tag="PK614_09435"
     CDS             1098..1343
                     /locus_tag="PK614_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82467.1"
                     /translation="MAEQGKGLYDHQYGRFRENQGTHQGYEFQHGPAGSVPPPVVTFM
                     DKIKWWTLRGWRRKKILAERDRFQQDIIRIKQTQPKS"
     gene            1433..>2059
                     /locus_tag="PK614_09440"
     CDS             1433..>2059
                     /locus_tag="PK614_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82468.1"
                     /translation="MAHLRNIRRYAGVIVLTLMSVSGVESVLAVPKETSTEKAEHLRQ
                     QANPQLFNNWTFDKDQAGGVPKGFLAVASGDQPGGDWKVEAQSAAPSSPNVVLGASSC
                     PACVELLVAQEFQYEYPELVVRIRQGADATSGQIGVVFGLKDPKNYYATVVDLSQKSI
                     EVVRVIEGKESILGRAAIKSKPVEWHTLRVQRNTIISKDFVETFFDGSL"
BASE COUNT          447 a          518 c          677 g          417 t
ORIGIN      
        1 gatggttgac tctcagcgat ccctccaatc tggagcagtg aatcatcgtc gatctgagtt
       61 atgcggacgg gggcggactg aatctgggtg tgcgcgggga gtcgatcggg cggggcggga
      121 gactcggagc ccggtgagcg atgctgttgt agctccagga atcgttgccg cacgaactcc
      181 gcggaagcaa ggtcgtcagg cgtcccggtc tggcgtccgt tgaatgccgg ttcgctcaac
      241 gcgcggatat ccgccagcat gcgctcactg gaaatctgac tcaatgcctg cgtcagcgcc
      301 gtggacgggg cgcccgtttc tgcggcgata ctcgggccgg tggccagggc catacataag
      361 atgaacagca ggtggaacga gagtcgatcg atgtatgacg attcagtgcg cctggtgttt
      421 cgccggcaag gcgcaagtgg tatggagcga tcgcagggca tcagatagac gtggcagagg
      481 ccttttgtgc cgaccagcgt tgataccatc gccagagcgg tggctgcaga aaacgcggaa
      541 tcggataacc caggccacgg acatagccat cgtctcgatc cagaactgcg aatgcccacc
      601 aatatttttc ttgaaatgct gcgcagcgtg tccggatttc ctcatcactg agcggaatgt
      661 gttgacgaat ggctgcgcct atcgcctcat cggaggtcat ctcacgaacc agcggctcga
      721 tatgggcggg cgtgaaccgg aggtgtcgca tgaaggattg gtccagtgcg gaaggatagg
      781 cataggttcc aagcgtgccg gcctgaaacg cgcgcacctt gtcgatcaac cggggcagcc
      841 acatcatgcc tgccagggtg tcactccatc gtcgcggcgg ctgctgggat aggtccatac
      901 ggtgcctcat aatcctgggt aggagaggtt tcttgcgaac cgtaggcact atagcatacc
      961 cggtcgatcc cattgcagag agccgagggc tcaaggtact atcggccatc aagtgagcgt
     1021 cggacccttt ctttcaggag ccgatactct gtgggacgcc gggcacagat cgacgagcga
     1081 ggcaagaggt aggatctatg gcagagcagg gaaagggtct gtacgaccat cagtacggac
     1141 ggtttcgcga gaatcaaggc acacatcagg gctatgaatt tcagcacggg cctgccggga
     1201 gcgtgcctcc ccccgtggtg acgttcatgg acaagataaa gtggtggacg ttgcgcggct
     1261 ggcgacgaaa gaagatcctg gctgaacgcg atcgatttca acaggacatc atccggatca
     1321 agcagaccca gccgaaatcg taagatccgc tcgaaggagg agtgctcccg tgcaatatgg
     1381 acacgagctg gtaaggcaga tagagttcct acgtgacagt cagttgcttg ctatggcgca
     1441 tctcaggaat atcaggagat acgccggtgt catcgtgttg accctgatgt cggtgagcgg
     1501 ggtggaatcg gtgctggcgg tgccgaagga aacgtctacg gaaaaggcgg agcatttgcg
     1561 gcaacaggcg aatccccagc tcttcaacaa ttggacgttc gacaaggatc aggcgggagg
     1621 ggttccaaag gggtttcttg ccgtggcttc gggcgatcaa ccaggcgggg actggaaagt
     1681 tgaagcgcaa tcggcggcac cttcctcgcc caatgtcgtg ctgggggcat cttcctgtcc
     1741 cgcctgtgtc gagttgttgg tcgcgcaaga atttcagtat gagtaccctg agctggtcgt
     1801 ccggattcgt cagggagccg atgccacgtc cgggcagatt ggagtggtgt tcggcctgaa
     1861 agatccgaag aattattatg cgaccgtggt ggatttgtcg cagaagtcta tagaggtggt
     1921 gcgggtgatc gaaggcaaag agtcgatttt aggccgggcg gcgattaagt cgaagccggt
     1981 tgaatggcat acgctccggg tccaacggaa caccatcatc agtaaggatt ttgtggagac
     2041 cttcttcgac gggagcctg
//
LOCUS       DAORQN010000255         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1490_length_2056_cov_5.910545, whole genome shotgun sequence.
ACCESSION   DAORQN010000255 DAORQN010000000
VERSION     DAORQN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1490_length_2056_cov_5.910545"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..819
                     /gene="lexA"
                     /locus_tag="PK614_09445"
     CDS             268..819
                     /gene="lexA"
                     /locus_tag="PK614_09445"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRB82469.1"
                     /translation="MMTSSEFRQIREQLGLTQEQLAAALRTTRVSVARYESGMRRISG
                     AVQVAITQLANRTQIPMAGVVAAGLPIEPVTQSEWVEVPPGMVRRGETFALRVKGESM
                     IEDGILPGDLVVVRKQATAQNGQTVVALVNREATIKTYFKHARHIELHPANAAMKPFV
                     VTPEDEFSIEGVLVGVIRHCENA"
     gene            834..1085
                     /locus_tag="PK614_09450"
     CDS             834..1085
                     /locus_tag="PK614_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82470.1"
                     /translation="MMMSHQQQAMVERVDELERMIVRLNGRIGELQRELKHTKGMTFQ
                     GWSGGSRLRTLLGGMKPVVSASHPIRENRRIERHDLCLF"
     gene            1066..1248
                     /locus_tag="PK614_09455"
     CDS             1066..1248
                     /locus_tag="PK614_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82471.1"
                     /translation="MISVSSDLDAVTAGCCADCGQLVLRRTPWFDEKAKAVREVCIRC
                     LVLTYQRRQFEAGCCG"
BASE COUNT          443 a          484 c          640 g          489 t
ORIGIN      
        1 atctagtagt ggctctctta tgtgtatgta ttagcattca tatttgtcca ggactccagg
       61 tgttttgaaa atgagctgtg gatagtggct ttattttttt tgaccatcca tatggctctt
      121 gacttatgca cagaaggttt tcgtttctga tgaaattaca ggggctcgga tcgcaaaaac
      181 cgcactggaa gaggccgcca gaatagtcaa gaaagatgat aggcagatgc ttgacagaga
      241 ctcaatttat gtgtaaccat cggttacatg atgacatcaa gcgagtttcg acagatccgt
      301 gaacagttgg gacttacgca ggagcagctt gccgcagcgc tgcgcaccac gcgggtgtcg
      361 gtcgctcgtt atgaatccgg catgcgtcgg atcagtggcg ccgttcaggt agcgatcacg
      421 cagttggcca acaggactca gattcccatg gcaggggtcg ttgctgccgg tttgccgatc
      481 gagccggtga cccagtcgga atgggtcgag gtacctcccg gcatggtgcg acggggggag
      541 acctttgcgc tccgtgtgaa aggggagtcg atgatcgagg acgggattct cccgggcgac
      601 ctggtcgtcg ttcgcaaaca ggcgacggca cagaatggcc agacggtcgt ggcgctcgtg
      661 aaccgggaag cgaccatcaa gacctatttc aagcacgccc ggcacattga acttcatccg
      721 gccaacgctg ccatgaaacc gtttgttgtc acgcctgagg atgaattttc catcgaaggg
      781 gtgctcgtcg gtgtgattcg tcattgcgag aacgcgtaat tcaaggagga ataatgatga
      841 tgagccatca gcaacaagcc atggtggagc gtgtggacga actggagcgc atgattgtcc
      901 ggctcaatgg acgcatcggg gaactacagc gagagctgaa gcataccaag gggatgacgt
      961 tccaggggtg gtccggcggc tcgcgcttgc gaactcttct cggcggaatg aaaccggtcg
     1021 tgtccgcctc acatcccatt cgagagaata ggaggattga acgccatgat ctctgtctct
     1081 tctgaccttg atgcggtgac agctggatgt tgcgctgatt gtgggcagct ggtgttacgg
     1141 cgaacgccgt ggttcgatga gaaggcgaag gcggtgcggg aggtgtgcat ccgttgtctg
     1201 gtgctcacct accagcgccg gcagtttgaa gctggttgtt gcgggtagtc tcgggggcag
     1261 gttacccgga ccggcctggc tttcggcaga gcgttcacgt gagctgccgt tcggctatga
     1321 tcactagttc agtcgagcga tgatcgctcc tatcgaatct ccttgcttgt agtttcgctt
     1381 ggagctaata ggatggctct tgtgacaatc gacgcaggct tgtgcgatgg aaaagtccgc
     1441 tgaactcgct ctgaattggt tccctccgaa aacgtcagga gctttagctg tggattgctc
     1501 agcagctttt tgtgtgcccg gcttcgactg cggttgggcc ggtttgactc attaataggc
     1561 gtcaagtcat cagatcacgt cagaaatccc tggttttcgc ttctgctgct ttgctgaact
     1621 ggaagggcag catgatcgca tgatcatcgt aagtccactc tgacgcccgc ctgtttggtt
     1681 cgttccgcga cgctgttggc ctagacgggt tggaaatcca agaatcgggt aggtgctgtg
     1741 agcgattcgc tgttcactga gtacaagatg aacaggggtg aatggcgaag gggaatagta
     1801 agcttagaaa tggtggtggt gaccaggttt gaacgggtga cccgcgactt atgagttgta
     1861 tcggtatgcg cgagaagcac tggaatatgc atgaacacag gccctgaccc agacaggggg
     1921 gcgctaagga tacggcagcc gtaagcaccc tggcaccctc ggcgcaccct cttctgaaag
     1981 atagctaggg gtgtacccgc ttataaaaga cgggggggta ccggggattt ttgatcacgg
     2041 aaactgcgtt ggccat
//
LOCUS       DAORQN010000256         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1496_length_2050_cov_4.288221, whole genome shotgun sequence.
ACCESSION   DAORQN010000256 DAORQN010000000
VERSION     DAORQN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_2050_cov_4.288221"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK614_09460"
     CDS             complement(<1..195)
                     /locus_tag="PK614_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRB82472.1"
                     /translation="MAAAAPKHFSMLREFHLADVLTLGNAACGLAAVFFAMHYMGSGS
                     PTHFFAATTLAPAALLFDWLD"
     gene            complement(353..670)
                     /locus_tag="PK614_09465"
     CDS             complement(353..670)
                     /locus_tag="PK614_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82473.1"
                     /translation="MMKRSVEMLLVAALVTGGGGLAFAADPMKEKEATPSIGERITKD
                     AVKGTLMKMDGEFYQIRNTDGELVKVHVDKSTKLDKVVEGDMVKAYVTDKGHVTTLQR
                     VEP"
     gene            complement(1194..>2050)
                     /gene="hflX"
                     /locus_tag="PK614_09470"
     CDS             complement(1194..>2050)
                     /gene="hflX"
                     /locus_tag="PK614_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRB82474.1"
                     /translation="LAPRLRETGGGSERQGGGVGGKGAGETSLELDKRRIRDRTKELR
                     TELAAIGDEHQTRRARREHELTVALVGYTNAGKSSLMRAMTGIEVLVADKLFATLDTT
                     IRPLYPDTRPKVLMSDTVGFIKKLPHDLVASFKSTVDEAASASLLLFVVDASDPSFRS
                     QLDVTRKVLAEVGATDVPSLLVLNKRDRLGPDELAALKAEYPDAFFLSTRNKDDLQAL
                     RERIMGYFESDMLDEELRIPFTAQGVVAEIRAKMRILSEEYDAEGLTIRVRSTPENLT
                     AIKKKLAR"
BASE COUNT          410 a          624 c          588 g          428 t
ORIGIN      
        1 gtccaaccaa tcgaagagca gggccgccgg cgccagggtc gtggcagcga agaaatgggt
       61 cggagacccg ctgcccatgt agtgcatggc gaagaacacg gccgcgagcc cgcaggcggc
      121 gttgcccagc gtgagcacgt cggccagatg aaactcgcgg agcatcgaaa aatgtttcgg
      181 agctgcagcg gccacggcag gaaaatctac gcgggattgc gccgaatgtc cagaatctga
      241 attctgaagc gagaggagtg cggtcaagca gaacttcctc tcgtctccac cgcctgaccg
      301 agcggggcgg caggctcgca agcccaccgc cccgcactgc ttggaaatgg gattagggct
      361 caacccgctg caacgtggtc acatggcctt tatcggtgac gtaggctttg accatatcgc
      421 cttcgaccac cttgtcgagc ttcgtgctct tgtccacatg cactttgacg agttccccgt
      481 ctgtatttct gatctgataa aactcgccgt ccatcttcat gagggtgccc ttgacggcat
      541 ccttggtgat ccgctcaccg atgctcggcg tggcttcctt ctctttcatg ggatcggccg
      601 caaacgcgag tcctccccct ccggtaacca gggctgcgac gagcagcatt tcaacacttc
      661 gtttcatcat acaccccccc atgttgtaac tgagtcagtg tcaactctta ccgtaaccct
      721 catgagaagg aggacatact gggaaaagcc cctgacacag ggcctcgctg gatcatgtgg
      781 aacttgctga cagggagggg ctagcgacag ggggaacgca aggttggatg cacggggcgg
      841 aggcccccac aggttcctat agttttgcga aggcctcgtc gcaccgccgc gccatgaaaa
      901 agtctttctc cgtcaatccg ccttcatcat gggcggtaag actcaacgtg agcttgttga
      961 accggatgcc gatatcgggg tggtgctggc tcttttcggc gcgccttagg agaatctcct
     1021 tttaggaatg tgtgtttcga tcgcttctgc gtgcgactgg attgggcaga cagcgagagt
     1081 aaggctcgcg caattctgcc ccacccgacc ggacgaagga gcgagtcaac tcccgaggat
     1141 cggccgattg ttcgattgca agacctgacc ccaaataggg tggaatccgc gcctcatcgc
     1201 gcgagcttct ttttgatggc agtcaggttc tcaggggtcg atcgcacccg tatcgtgagt
     1261 ccttcggcat catattcctc ggacaggata cgcatcttgg cgcggatctc cgcaacgacc
     1321 ccttgagcgg taaagggaat gcgcaattcc tcgtcgagca tatcgctctc aaagtacccc
     1381 atgatgcgct cacggagcgc ctgcagatcg tccttgtttc tcgtggacag gaagaacgcg
     1441 tcgggatatt ccgccttcag tgccgcgagt tcgtccggcc cgagacgatc ccgtttattc
     1501 aacaccaaca ggctgggaac atccgtggcg ccgacttcgg ctaacacctt ccgcgtgacg
     1561 tcgagttggg atcgaaagga gggatctgac gcatcgacga caaacagcag gagcgaggca
     1621 gaggccgctt catccactgt cgacttgaac gacgccacca agtcatgcgg gagctttttg
     1681 atgaatccca ccgtatcgga catgagcact tttggacggg tgtcaggata caagggccgg
     1741 atcgtggtat cgagcgtggc aaacagctta tcggccacga gcacctcgat gcccgtcatt
     1801 gcacgcatga gcgaggattt cccggcattg gtgtacccca cgagcgcgac cgtcaattcg
     1861 tgttcccgcc tcgcgcggcg cgtctgatgc tcgtccccga tcgccgccaa ttccgtccgg
     1921 agttctttcg tgcgatcgcg gattctgcgc ttatcgagct ccagactcgt ctccccggcc
     1981 cccttgccac cgactccccc gccttgccgc tcgctgccgc cgccggtttc ccgcaaacgc
     2041 ggcgcaagat
//
LOCUS       DAORQN010000257         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1499_length_2049_cov_6.726680, whole genome shotgun sequence.
ACCESSION   DAORQN010000257 DAORQN010000000
VERSION     DAORQN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_2049_cov_6.726680"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1138
                     /locus_tag="PK614_09475"
                     /pseudo
     CDS             5..1138
                     /locus_tag="PK614_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65"
     gene            complement(1298..1633)
                     /locus_tag="PK614_09480"
                     /pseudo
     CDS             complement(1298..1633)
                     /locus_tag="PK614_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(1741..>2049)
                     /locus_tag="PK614_09485"
     CDS             complement(1741..>2049)
                     /locus_tag="PK614_09485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRB82475.1"
                     /translation="NYHCIFLDCAPIAIGDDVQIGPAVQLYTAQHPIDAKVRRSGLES
                     ASPIRIGNDVWIGGGAVVLPGVTIGDRSIVGAGSVVVHNVPPDSLVVGNPARIVRTLV
                     "
BASE COUNT          398 a          628 c          627 g          396 t
ORIGIN      
        1 ctatgtgggg acgcggggtt cgctcgccga acgcacgagc ctctcctccc ctgccacctt
       61 cctggcagga gcgtttgagg cctctgaggc cccgggtggc accctggagc tcacgaagct
      121 cccggattgg acgcagcttc gcgtgctggt ggaaggggtc ccgctgtcgc tcgaaaccgg
      181 aaccacgctc gatcaccgac gcatcctcga tctgcagcat ggtctgcttt ttcgaacctg
      241 gcggcacgag gatgagcagg gccgagtgac ggccctcacc tgcctgcgcg ccgtgtgtca
      301 tcacgatcga cacctcctgc tccaatcggt cacgatcacg ccagagaatt attccggtac
      361 catctcgatc gaatctttca ctgaagggcc tcagccgggg acacaatggt ctttcagggt
      421 ggagggcgag gcagccgtga tcgtgaggcg gacggacagg ggaacgacga tcgccatggc
      481 ctctcataac aagctgcgat atgagaacag ccaggaggga gcgggcaata gcccggtcga
      541 aggatttgct gagcagtgga cgtggcaggc caaaaggggc gaggcggccc ggttcgatcg
      601 ctcggttgtc gtctattcaa cccgcgaggt acgggatcct gctgttgcag ccagcgaaca
      661 tgtttgccga gtctccgagg cggggctgga tgcccacatt ctgcaacatg cggaagcctg
      721 ggaggatcgt tggcgtgcca gcggagtgga ggtagccggc gatgagatgg cgcagcgcgc
      781 gctccgcttt gccctctatc atctcaatag cgccgtgaat ccgaaggacg agtatgcgtc
      841 cgtcggagcc agagctctca ccggctccac ctacaagggg cacgtcttct gggatacaga
      901 aatctatctg ctgccgtttt acacttttac cgatcctccg gcggcgcggg ctcttttgca
      961 gtaccgtttc catactcttc cggctgcccg cgacagggcg cggcggctgg gatatcaggg
     1021 agctttgttc gcctgggagt ccgccgatac cggagaagat gtcacgccgg atacggtcat
     1081 cgcgccggat ggccgggtgg ttcaagtgct cacaggaatt caggaacacc atatcagcgc
     1141 cgatgtcgcc tatgcggtct ggcaatactg gcaggcgacg gcggatgagg cctttttcct
     1201 cgcgcagata atccggcaac gatgccgggt cgccagggta tccgacggcg atcatcgcca
     1261 ccggttcata cccggcggga atgccgagat cggtacgggc cttctctatc tcaaatcccg
     1321 ccatttggtg agtcaccaat cccagcgccg tcgcttgcaa gcagaggctg agcgcggcca
     1381 tccccgtgtc gtgccaggca tgccggtttg gagtgccttc gtcctcaaaa ttcagccgtg
     1441 cgacggacaa tatcaagacc ggggcgcgaa acgcccactt cctgttgccc tccttgaggc
     1501 aggccacgag acggtcgtgc gcggaaggat ccgctttgat tccgacaatg aagtgccagg
     1561 gctgctcgtt attcgacgac ggcgcccacc tcgccgcctc gaacaggctc tgcagttgtt
     1621 ccggctccac catccggtcc gcaaacgcgc gcggactcca ccgccgctgt aacagagcat
     1681 gaatggggtg ggtcacatca gcaggttttt ccatgaacga atccttctcc agatcggaat
     1741 ttacaccaac gttcgcacaa tgcgggctgg attgccgacg acaaggctat ccggaggcac
     1801 gttgtgcacg accacgctgc ctgcgcccac gatgctacga tctccgatcg tgacgcccgg
     1861 cagcaccacc gccccgccgc caatccacac atcgttgccg atccgaatcg ggcttgcgga
     1921 ttccaggccg gagcgacgca ccttggcatc aatgggatgt tgcgccgtat agagttgcac
     1981 ggcagggccg atctgtacat cgtctccaat ggcgatagga gcacagtcaa gaaagatgca
     2041 gtgataatt
//
LOCUS       DAORQN010000258         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1504_length_2044_cov_5.574661, whole genome shotgun sequence.
ACCESSION   DAORQN010000258 DAORQN010000000
VERSION     DAORQN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1504_length_2044_cov_5.574661"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..814)
                     /locus_tag="PK614_09490"
     CDS             complement(164..814)
                     /locus_tag="PK614_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB82476.1"
                     /translation="MGLKPATNPDVEAATIELSIDGRTVSAKDGVSLYDVIASTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVIRTDGDDLFAARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSLVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGPLPVNSAETAWLSVRSARSRTNIPSTCTSPGR"
     gene            complement(895..>2044)
                     /gene="nuoD"
                     /locus_tag="PK614_09495"
     CDS             complement(895..>2044)
                     /gene="nuoD"
                     /locus_tag="PK614_09495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRB82477.1"
                     /translation="LNMAPQHPSTHGVLKVILELEGERLVKSTPLMGFLHRGVEKLAE
                     EGTYHQFIPHTDRLDYVCAMYNNFAYCRAVEKLMNITVPDRAEYLRTIVAEIQRIIGH
                     QFWLGTQALDIGAMTVFFYCFRDREILLDWFDELCGARLTTSWYRIGGVERDFTPSLF
                     AKLKQFLDYFPPKIDEYVIFLEKNRIWLARTKGVAVISAEDALSFGLSGPTLRGSGVD
                     YDLRKYEPYSAYPKCEFSVPVGKNGDTYDRYWIRVQEIYESVKIIRQCLEQIQDGPIM
                     ADVPSVTLPPKERVFTNLESMIQQFKLFSQGFNAPPGEIYCGTEAHKGELGFYIVSTG
                     GGKPYRLKIRAPSFIHMGAFDHMSKGYMIADAVTIFGTYDIVMGECDR"
BASE COUNT          418 a          561 c          593 g          472 t
ORIGIN      
        1 ccgcgaacgc agagtccccc gtgtcccttg gcagtttcag tccggtcgcc ccacttattt
       61 ttccaggaga gcggcgaggt gatgcggacg acttcggtat ctttggtttc gagatgcatc
      121 tggcagccat cgccacaata gttgcaggtg gtggtggttt ttttcatctg ccagggcttg
      181 tacaggtact tggaatattt gttcgtgatc gcgccgaccg gacagacagc caggcagtct
      241 ccgcagaatt cacaggcaag gggcccttct tcatgaactg caacgcgtcg atcatcagca
      301 cgtctttgca gacgttgatg cattccgcgc aggcgatgca gcgattcatg ttgaagtcga
      361 ggacgagact ccgcgtgtct tcaggaatga acttttgctt cgcattggcg agattggtca
      421 cgccatgctg gaaggccatg tcctggagct cgcagtgccc gtccgcgtcg cagaccgggc
      481 agtccaacgg gtgaaccgag agatgttttt cgacggcttt ctttcgtgcc gcgaagaggt
      541 cgtcgccgtc agtgcggatc accataccgg ctgccgcctt ggcagtgcag gaacggaccg
      601 gggccttctt gccttcctgc atgaccaggc acataccgca ggatccgaag ggatcaaatg
      661 tatagtgata acacatggca ggaataatct tgcccgtgct ggcaatgaca tcgtacagcg
      721 agacgccgtc tttggccgat accgtccgtc cgtcgatcga cagttcgatc gtggctgctt
      781 cgacgtctgg attggttgct ggtttcaaac ccatgaagtt acctcaagtc aaaagcgatg
      841 gggcgaaaga atgaatcagc gccgctcact ttttcctttg tgccgtatcg gtcttcatcg
      901 atcgcattcc cccatgacga tatcgtacgt gccgaagatc gtcacggcgt cggcgatcat
      961 gtagcctttc gacatatgat cgaatgcgcc catatggata aacgacgggg cgcggatttt
     1021 cagccggtac ggcttcccgc cgcccgtgct gacgatgtag aaacccaatt cgcctttatg
     1081 cgcttcggtc ccgcagtaga tttctccggg aggcgcgtta aacccttgcg agaagagttt
     1141 gaactgctga atcatggact ccaggttcgt gaacacgcgt tctttcggag gcagagtcac
     1201 gctcggcacg tcagccatga tcgggccgtc ctggatctgc tcaaggcatt ggcggatgat
     1261 cttgacgctt tcgtagatct cttgcacgcg gatccagtac cggtcatagg tgtcgccgtt
     1321 cttgccgacc gggacgctga attcacactt gggataggcg gagtatggct cgtatttacg
     1381 aagatcgtaa tcaacgcctg agcctcgtaa cgtcggtccg ctcaagccaa agctcagtgc
     1441 atcttcggcc gaaatcacgg ccacgccttt ggtgcgcgcc agccagatgc ggttcttctc
     1501 caggaagatc acgtactcat cgatcttcgg tgggaaatag tcgaggaatt gtttcagctt
     1561 ggcgaacagg gacggtgtga aatcgcgctc gacgccgccg atacgatacc agctcgtcgt
     1621 gaggcgagca ccgcacagtt catcaaacca atcgagcaga atttcacggt cccggaagca
     1681 atagaagaag accgtcatcg ccccgatgtc gagggcctgc gtgccgagcc agaactggtg
     1741 gccgataatg cgttggattt ccgcgacgat cgtgcgcagg tactcggcgc ggtccggcac
     1801 ggtaatgttc attagctttt cgacggcgcg gcaatacgcg aagttgttgt acatcgcaca
     1861 cacgtaatcg aggcggtcgg tgtgcggaat gaactgatga tacgtgcctt cttccgccag
     1921 cttttctaca ccgcggtgga gaaagcccat cagcggggta gacttgacca accgctcgcc
     1981 ttccagttcg aggatgactt tcagcacgcc gtgcgtgctg gggtgctggg gcgccatatt
     2041 gagg
//
LOCUS       DAORQN010000259         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1523_length_2015_cov_4.385714, whole genome shotgun sequence.
ACCESSION   DAORQN010000259 DAORQN010000000
VERSION     DAORQN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_2015_cov_4.385714"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1422)
                     /locus_tag="PK614_09500"
     CDS             complement(109..1422)
                     /locus_tag="PK614_09500"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRB82478.1"
                     /translation="MEQDLTESPVPELDAPVEVLTPEEYVATLVKRAKGAAGRLSSLP
                     TAVKNQALEAMADGLEEHETELLAENEKDLEQFEATPERKALGDRLRLTAERIREMAA
                     GIRDIARLPDPLGDTPKMWTRPNGMQVGRMRVPIGVIGIIYEARPNVTADSAALCLKS
                     GNVCILRGGSEALHSNVAIAKVLSESAEKAGVPAGAITFVDRAEREVVQLLLKQDRYI
                     DLIIPRGGESLMKTIAEHATIPVLKHDKGVCHTYVDVDADPQVAERICLNAKVQRPST
                     CNAMETLLVHHSIARTWLPLLVEKLKAAKVDVRGCAKTCQLCPEVTPAAEDDFGREFL
                     DLILAIKVVKNMEEALEHITTYGSQHTEAIVTKDYARAMRFLREVDAGAVLVNASTRL
                     NDGYQFGLGAEIGISTSRLHARGPMGLEELTCSKFVVLGSGQIRE"
     gene            complement(1547..>2015)
                     /locus_tag="PK614_09505"
     CDS             complement(1547..>2015)
                     /locus_tag="PK614_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82479.1"
                     /translation="REKEADLMAKGIEIQNAIALYSNTMKIGRILPSEIYPQSLAELT
                     RTPKPYLRRVYQDPMSGGDWEYMRAPTGGIMGVRSKSKAKPIKERDFPLAVRHFEGRK
                     TYHDWMFQHPNPSSMSMLMPPMMGLAPGTMPMQPGPQGSAPGAPPTPGPARNP"
BASE COUNT          383 a          620 c          581 g          431 t
ORIGIN      
        1 aacggatccc gccacagccg tcaggtgtgg caggtccgtg agattggaag gaaaggtgag
       61 cgtgccggga cgcctgagaa tctcgtgtac caaggaatca ggcggcattt actcgcggat
      121 ttgaccgctc ccgagcacca caaattttga acaggtcaat tcttcaagcc ccatcggccc
      181 tcgagcgtga agcctcgatg tgctgatgcc gatttccgcc cccaggccga actggtagcc
      241 gtcgttgagc ctggtcgagg cattcacgag caccgcgccg gcatcgacct cgcgcagaaa
      301 ccgcattgcg cgcgcgtagt ccttggtcac aatcgcctcc gtatgctggg atccgtaggt
      361 ggtaatgtgc tccaaggctt cttccatgtt cttgaccact ttgatcgcga ggatcagatc
      421 cagaaattca cgaccgaaat cgtcctcagc ggccggcgtc acttcaggac agagctgaca
      481 ggtcttcgcg caaccacgga cgtccacctt cgccgctttg agtttctcca ccaggagcgg
      541 cagccaggtg cgcgcgatac tgtggtggac caacagggtc tccatggcat tgcaggtcga
      601 cgggcgctgt actttcgcgt tcagacagat ccgctcggct acctggggat cggcatccac
      661 atcgacatag gtgtggcaga ctcccttgtc gtgtttgagc accgggatcg tcgcatgttc
      721 ggcgatcgtc ttcatcagcg attccccgcc tctcggaatg atgagatcga tataccggtc
      781 ctgtttgagc aacagctgga cgacctcacg ctccgcacga tcgacgaatg taatcgcgcc
      841 cgccggcacg ccggccttct ccgccgattc gctcaacacc ttggcaatgg ccacgttcga
      901 atgcaaggct tcggatccgc ctcgcagaat acagacgttt ccggacttga gacacagggc
      961 ggccgaatcc gcggtcacat tcggacgcgc ttcataaatg atcccgatga ccccgatcgg
     1021 cacacgcatg cggcccactt gcatcccgtt cggccgcgtc cacatcttcg gcgtatcgcc
     1081 gagcggatcc ggcaatcgcg caatgtcacg aatccctgcc gccatctccc ggatacgttc
     1141 cgccgtcagc cgtaaacgat cccccaaggc ttttcgctcc ggcgtggcct cgaattgttc
     1201 gagatccttc tcgttctcgg ccaacagctc cgtctcatgt tcttcaagcc cgtcggccat
     1261 ggcttccaag gcctgattct tgaccgccgt cggaaggctg gagagccggc cggccgcacc
     1321 cttggccctt ttgaccaggg tcgcgacata ttcttccgga gtgagtactt caacgggagc
     1381 atccagctcc ggaaccgggg attcggtgag atcttgttcc atgtcgtgaa ttcctgctgc
     1441 tggccacaca tgaggcccga tgcctcagcg tatcggccga tcaagatggt gaacgaaggc
     1501 gcggcgatct atagccagac aggatactgt ggggttcgga gtcgggtcaa gggttgcgag
     1561 caggacccgg tgtcggcgga gcacccggag cagacccctg cggccctggt tgcatcggca
     1621 ttgtgccggg agccaggccc atcatgggcg gcatcaacat ggacatcgat gagggattgg
     1681 gatgttgaaa catccagtcg tgatacgttt tccggccttc gaagtgacgg accgcgaggg
     1741 ggaaatcgcg ctccttgatc ggtttcgctt tacttttgct ccggacgccc atgatgccgc
     1801 cggtcggcgc gcgcatatac tcccaatcac cgccgctcat cgggtcttga tagaccctcc
     1861 gcagatacgg cttgggcgtt cgcgtcaact ccgccaggga ctgaggatag atttcggagg
     1921 gcaggatgcg cccgatcttc atcgtatttg agtaaagggc aatggcattt tgaatttcga
     1981 tgcccttggc catgagatcg gcttctttct cgcgc
//
LOCUS       DAORQN010000260         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1529_length_2004_cov_7.129810, whole genome shotgun sequence.
ACCESSION   DAORQN010000260 DAORQN010000000
VERSION     DAORQN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1529_length_2004_cov_7.129810"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK614_09510"
     CDS             <1..369
                     /locus_tag="PK614_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HRB82480.1"
                     /translation="TNAAHSHPGSFWAGVYYVDVGEVCPTLGKGGELQIYDPRGCLPR
                     MLAPYLQYSMTELHDAGTSISYSPAAGQCLLFPGWLFHAVNTYRGTAPRISVAFNLDP
                     VLQQGPLSSAHADAVGGSRR"
     gene            complement(436..1026)
                     /locus_tag="PK614_09515"
     CDS             complement(436..1026)
                     /locus_tag="PK614_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82481.1"
                     /translation="MARPAMYRCRRSNNCMVVVGLALLALTAGCSPYEWRHENQWESR
                     DQTWLAEASQVKVRAAQSRVFDTADRLKTIQGVVATCQDLGFAVEVLDETLGIVSGKK
                     FDDYESLPWGDPSYHLYRDDGLLILTRQFRSWGPFYHRSNLVRLTVTVRPRGNRQVIV
                     RASAQYYLKAIESPEPYQQFFRALEQALFIQANALP"
     gene            complement(1030..1467)
                     /locus_tag="PK614_09520"
     CDS             complement(1030..1467)
                     /locus_tag="PK614_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82482.1"
                     /translation="MNWTQLVGAVVVMMGSVILQGCVAPEPRQELFAPTDAQMKIRSA
                     QTRTFEVKEQTVAMRGVIAALQDLGFIIERANEPLGLVTAARFAEPNYYDVLGVTVTV
                     RPQQDGKTMIRANAIYNSKPIEDPKVYQNFFAAVERSLFLVKD"
     gene            complement(1480..>2004)
                     /locus_tag="PK614_09525"
     CDS             complement(1480..>2004)
                     /locus_tag="PK614_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82483.1"
                     /translation="PQGCVQQTQPAELFQLTPESSANRAMQTRFFDTENDQELLSASA
                     AALQDLGFQVEESVREVGFLRAAKERSAREYGQYRNRFFIWLLSLGHVVIPIDLHQKI
                     AASLVTRPLNEAHSRQEVRIVFYRAVWKGDGQADRNYIPPGEQKMEMIRDPEIYQQFF
                     AKLSKAVFLEPHSI"
BASE COUNT          397 a          626 c          573 g          408 t
ORIGIN      
        1 accaatgcag cccactctca ccccggaagt ttttgggcgg gcgtctatta tgtcgatgtg
       61 ggcgaggtct gcccgaccct gggaaaggga ggcgaattgc agatttacga cccgcgaggc
      121 tgtctgccgc gtatgctggc gccctatctc cagtacagca tgaccgaatt acatgatgcc
      181 ggaaccagca tttcctactc ccctgcggcc ggacaatgcc tgctgttccc tggctggttg
      241 ttccatgccg tgaatacgta tcgtggaacg gcgccccgaa tcagtgtggc gttcaatctc
      301 gatcccgtct tacagcaggg ccccctgagc agcgctcacg cggatgccgt cggcggatca
      361 cgccggtgag agccccctcg ccgcagcctg cgcagctcgc acgcaggcga tgcgcatcgt
      421 ggtctccggc tctcgctatg gcagcgcgtt cgcctgaatg aagagagcct gctcgagcgc
      481 gcgaaagaat tgctgatacg gctccgggga ctcgatggcc ttgaggtaat actgcgcact
      541 ggcacgcacg atcacctgcc tgtttccgcg tggccggacg gtcaccgtga ggcggacaag
      601 attgctgcgg tgataaaacg gcccccagct ccggaactgc cgggtaagga tcaggagtcc
      661 gtcgtcccga tacaggtgat aggaaggatc accccatggc aaggattcgt agtcatcaaa
      721 ttttttgccc gagacaatgc ccagagtttc atccaacacc tccaccgcaa aacctaagtc
      781 ctgacaagtg gcaacaaccc cctgaatcgt cttgagacga tcagcggtat cgaagacgcg
      841 gctttgagcc gcccgcacct tgacctggct ggcctcggca agccaggtct ggtcgcgcga
      901 ctcccattgg ttctcatgtc gccactcata gggcgaacag ccggcagtca aggccagcaa
      961 ggcgaggcca accacgacca tgcagttgtt cgaccggcgg catcgataca tggcggggcg
     1021 agccaccgac tagtccttca ccagaaataa cgaacgttcc accgccgcga agaagttctg
     1081 atacaccttg ggatcttcga tcggcttgct gttgtagatc gcattggcac ggatcatcgt
     1141 cttgccgtcc tgttgtgggc gcacggtcac ggtcacgccg agcacatcgt aatagttcgg
     1201 ttcggcaaac cgggccgccg tcaccagccc gagcggttcg ttcgcgcgtt cgatgataaa
     1261 tcccaggtct tgcagagccg cgatgacgcc gcgcatggcg acggtctgct ccttgacctc
     1321 gaacgtgcgg gtctgcgcgc tgcggatctt catctgcgca tcggttggcg caaagagctc
     1381 ctgcctcggc tccggcgcaa cgcagccttg caagatgaca cttcccatca tgaccaccac
     1441 tgcgccgact agctgtgtcc agttcatgaa tcctcgtaat taaatcgaat gcggctccag
     1501 gaacacagcc ttggagagct ttgcaaaaaa ttgctgatag atctcagggt cccggatcat
     1561 ttccatcttc tgttcaccgg gtggaatgta gttgcggtcg gcctgcccgt cccccttcca
     1621 caccgcccgg tagaagacaa tccggacttc ctgccgcgaa tgtgcctcgt tgagcggccg
     1681 cgtcacgagg ctggccgcaa ttttttggtg caggtcgatc gggatgacga cgtgcccgag
     1741 tgacagcagc cagataaaga accggtttcg gtactgaccg tactcacgag cgctacgttc
     1801 cttcgcggct cgcaggaacc cgacttcccg cacactttcc tcgacttgaa acccgagatc
     1861 ctgcagcgcg gcagccgagg cagacaacaa ttcctgatcg ttctccgtgt cgaagaaacg
     1921 ggtctgcatg gcccgattgg cgctgctctc cggcgtgagt tgaaacaatt ccgccggctg
     1981 cgtctgttgc acgcagccct gggg
//
LOCUS       DAORQN010000261         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1535_length_1998_cov_4.785898, whole genome shotgun sequence.
ACCESSION   DAORQN010000261 DAORQN010000000
VERSION     DAORQN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1535_length_1998_cov_4.785898"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK614_09530"
     CDS             <1..350
                     /locus_tag="PK614_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRB82484.1"
                     /translation="PMVPLTATAAMINVDHAAVGNGRLTIGVTGLEKPAAQQAGERAG
                     LADRIDLFGFFPGGDHVPFKEAGVPTVTVVSGGIHPHFHQPTDTADTVNADILSAAAR
                     YILAIAWQLADAP"
     gene            complement(441..>1998)
                     /locus_tag="PK614_09535"
     CDS             complement(441..>1998)
                     /locus_tag="PK614_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRB82485.1"
                     /translation="ATFDIELDPKGETTVAVMVACDVGDSHRPLLAYDAAMAEAGLAF
                     GAEQTEDCTIQTSNAQFNEWWNRSVLDVRMMVTDTNEGPYPYAGVPWFSTPFGRDGVI
                     TALECLWIRPELARGVLAYLASTQAKEVNPGQDAEPGKILHETRKGEMAALNEIPFGL
                     YYGSVDSTPLFVMLAGAYYERTADLAFIQSIWANLEAALTWMDTFGDPDRDGFVEYVR
                     KSPTGLDNQGWKDSHDSISHEDGSLAEGPIALCEVQGYVYDAKVQASKLADALGYIDR
                     ASQLRRQARSLKERFEEAFWCEESSTYALALDGQKRPCQVKTSNAGHCLYTGIASDEH
                     ARRVAETLMSDELFSGWGVRTLADSERRYNPMSYHNGSIWPHDNAMIAVGLARYGLKS
                     GVEKIMTGMFEVSLVLDFHRLPELFCGFVRRPGQGLTRYPVACNPQAWAAGSAFMALQ
                     ACLGLSIIASEQKVVFNYPILPEFIDKMQIKNLKVGTASVDLLLRRHDLDVGITVIRR
                     AGKVEVVSVK"
BASE COUNT          420 a          645 c          545 g          388 t
ORIGIN      
        1 aaccaatggt gccgctgacc gctaccgcgg ccatgatcaa tgtggatcat gcggcagtcg
       61 gaaacggacg gctcacgatc ggggtaaccg gtttagaaaa acccgctgcg cagcaggccg
      121 gagaacgggc cggacttgcg gaccgtatcg atctgttcgg ttttttcccg gggggagacc
      181 atgtgccgtt taaggaagcg ggagttccca ccgtaaccgt ggtgagcggc ggaatccatc
      241 cgcacttcca tcagccgact gacacggcgg acaccgtcaa cgcagatatt ctctcagccg
      301 ccgcgcgcta catactcgcc atagcctggc aactcgccga cgcgccctaa cacttcagcg
      361 tactcgcagg tcgctctgca cccaaccgaa tgttatcgag ccaacgctca catcgcggcc
      421 gatcattcaa gcaggcgccg ctacttcacc gacaccactt caacttttcc ggcacgacga
      481 atgaccgtga tacccacatc gaggtcatgg cgccgaagca gtaaatcgac cgatgcggtc
      541 cccaccttga ggttcttgat ctgcatcttg tcgatgaact ccggcaggat ggggtaattg
      601 aacaccactt tctgctccga cgcgataatc gataggccaa gacaggcctg tagcgccatg
      661 aaggccgagc cagccgccca ggcttgaggg ttacaggcga ccggataccg cgtcaggccc
      721 tggcccggtc tgcgtacgaa tccgcagaag agttccggca agcgatgaaa gtcgagcacg
      781 agactgacct cgaacatgcc ggtcataatt ttttccacac cggacttgag gccgtaccgg
      841 gccagaccga ccgcaatcat ggcgttgtcg tgcggccaga tcgatccgtt gtggtaggac
      901 atcggattgt accggcgctc cgaatcggcg agggtccgca caccccatcc gctgaacagt
      961 tcatcggaca tgagcgtttc cgccacacgc ctggcatgct catcgcttgc gatccccgta
     1021 tagagacaat ggccggcgtt ggaagtcttc acctgacagg gccgtttctg gccatccagg
     1081 gcaagcgcat aggttgacga ttcctcgcac cagaatgcct cttcgaaacg ttccttgagc
     1141 gaccgcgcct gacgccgaag ctgactggca cgatcgatat agccgagcgc atctgccagc
     1201 ttcgacgcct gcactttggc gtcgtacaca tacccttgta cttcacacaa ggcgatcggt
     1261 ccttcagcca gcgaaccgtc ctcgtgcgaa atcgaatcat gggaatcttt ccagccttga
     1321 ttgtccaacc cggtgggtga tttccgtaca tactctacaa acccgtcacg gtctggatcg
     1381 ccgaaggtgt ccatccaggt cagcgccgct tccaggttgg cccatatcga ctgaataaac
     1441 gccagatcgg ccgtccgttc gtaataagcg ccggccagca tcacaaacag cggcgtggaa
     1501 tcgacactgc cgtagtagag gccgaaggga atctcgttca acgccgccat ctcacccttg
     1561 cgggtctcgt ggaggatctt acccggctcg gcatcctgac cagggttcac ctctttggcc
     1621 tgcgtcgatg ccagataggc caggacgccg cgcgccaatt ccggcctgat ccacagacac
     1681 tcgagcgccg tgatgacccc gtcccgtccg aagggtgtgc tgaaccaggg cacaccggca
     1741 tagggatagg ggccctcatt cgtgtcggtg accatcatac ggacatccaa caccgatcga
     1801 ttccaccatt cattgaattg ggcgttggag gtctgaatcg tgcaatcctc ggtctgctcc
     1861 gccccgaagg ccaggcccgc ttcggccatt gccgcatcgt acgccaacag gggccgatgg
     1921 ctgtccccca catcgcacgc caccatcacc gccaccgtcg tttccccctt cgggtcgagc
     1981 tcaatatcga aggtggct
//
LOCUS       DAORQN010000262         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1547_length_1977_cov_10.286681, whole genome shotgun sequence.
ACCESSION   DAORQN010000262 DAORQN010000000
VERSION     DAORQN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_1977_cov_10.286681"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1965
                     /gene="mrdA"
                     /locus_tag="PK614_09540"
     CDS             94..1965
                     /gene="mrdA"
                     /locus_tag="PK614_09540"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRB82486.1"
                     /translation="MASIGFNDSDLLDLQRRLVILRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVVLEPARGLIFDRNGVLLANNVPSFALYVTLEDVKDRPALVAQL
                     ASLLNLEPEVIQKKLSTGKGSKLQPRKVKDRLTLREATLIESHRLDLPGVMIQVESQR
                     NYPGGPVAAHLLGYVGEVSADQLEKADFADLHQGSVVGQYGVEKWFDRQVRGRAGQKS
                     VEVDALGHEKRAVVSDKPVAGDDLYLTIDARLQKTAEDLLGEESGAIVALDPTNGDIL
                     AMASRPGFDPNMLSKELTNKQWVEIVQNERRPLNNRATQGQYPPGSTFKVVMAAAALE
                     SNTVTPSTMVRCNGGYQFGNRLFHDWKQGGHGSVDMNEALIHSCDVYFYTVGQRMGID
                     TIAEYAKQFGLGQETGVELPSERVGIVPSTAWKQKARNQPWYPGETISAAIGQGYVTV
                     TPLQMASVIGTVANDGLSIKPRLVQAVMNRATGERAEFPPTVRGKVAVKPATIALIKA
                     ALADVVTKGTATRAKSSLVTIGGKTGTAQTAALRTGPEKDIPKRLRDHAWFVAFAPVE
                     APRIAVAVLAEHMGHGGSAAAPLAKDVIEAYVKAYPEVLNGVPAGGRTKSADARPKT"
BASE COUNT          398 a          530 c          664 g          385 t
ORIGIN      
        1 cgtcagcgtt ggtccgacga tcgaatggtg acggaggggg cgttgtaatc gcgtgcgggt
       61 gtcccggcaa caatcggaaa aagtctggcg accatggcct cgatagggtt taacgattcg
      121 gatctcctcg atctccagcg gaggctggtc attctgcgtg tgggcctcct gctggtcgtc
      181 gcgttgcttg ccttgcgtct gtggcatctc cagattcggg aaggccccta ttaccgggac
      241 ctgtctgaaa acaaccggac gcgctcggtg gtgttggagc cggcccgcgg tctcatcttc
      301 gatcggaacg gtgtgttgct ggcgaacaat gtgccgagct ttgccctcta tgtcacgctg
      361 gaagacgtga aggatcggcc ggccttggtt gcgcaattgg cctcgttgct caaccttgag
      421 ccggaggtga ttcaaaagaa gctgtcgacc ggcaagggca gcaagctgca gcctcgcaag
      481 gtgaaagacc ggctgacgtt gcgcgaggcg acattgatcg agtctcatcg gttggatttg
      541 cccggtgtga tgattcaagt ggagtcgcaa cggaactacc ccggcgggcc ggttgcggcg
      601 catctactgg gatatgtggg agaagtgtcg gcggatcaac ttgagaaggc ggactttgcc
      661 gatctccatc aaggcagcgt ggtcgggcag tatggagtgg agaagtggtt cgaccggcag
      721 gtgcgcgggc gcgcggggca gaagagcgtg gaagtcgatg cactcggcca cgaaaaacgc
      781 gccgtggtgt cggacaagcc ggtcgccgga gacgatctgt atctcaccat cgatgcccgg
      841 cttcagaaaa ccgccgaaga tcttcttggg gaggagtcgg gcgccatcgt cgcgctggat
      901 ccgaccaacg gggatattct ggcgatggcc agccgtccgg gattcgatcc gaacatgttg
      961 tcgaaagaac tcacgaacaa acagtgggtt gaaatcgtcc agaacgagcg gcgcccgctg
     1021 aataatcggg ccacgcaagg tcaataccct ccaggctcga cgttcaaggt cgtcatggcg
     1081 gcggcggcgc ttgaatccaa caccgtgact ccctcgacca tggttcggtg taacggcgga
     1141 tatcaattcg ggaatcggct gtttcacgac tggaagcaag gcggtcatgg gtcagtcgat
     1201 atgaacgagg ctttgatcca ctcgtgcgac gtctatttct ataccgtcgg gcagcgaatg
     1261 gggatcgata cgatcgccga gtatgcgaag cagttcgggc tcgggcagga gaccggagtc
     1321 gaactcccgt ccgaacgtgt tggaatcgtg ccttccacag cctggaagca gaaagcccgc
     1381 aaccagccct ggtatcccgg tgaaacgatt tctgccgcga tcgggcaggg gtatgtcact
     1441 gtgacaccct tgcagatggc tagtgtcatc ggtacggtgg ccaacgacgg cctgtcgatc
     1501 aaaccgcgcc tggtgcaggc ggtgatgaat cgcgcgacgg gagaacgggc cgaattccct
     1561 ccgacggtcc ggggcaaggt ggcggtgaag ccggccacca tcgctttgat caaggcggcg
     1621 ctggcggatg tggtgaccaa aggaaccgcg acgcgagcga agtcgtccct cgtgacaatc
     1681 ggagggaaaa ccggaacggc tcaaacggcg gccctccgta ccggtccgga gaaagatatt
     1741 cccaaaaggc ttcgggacca tgcctggttc gtcgcttttg ctcccgtgga ggctccgcgc
     1801 attgccgtgg cggtcctggc tgagcacatg gggcatggcg gatccgccgc ggccccgttg
     1861 gcgaaggatg tgattgaagc ctacgtgaag gcttatccgg aggtcctcaa tggagttcct
     1921 gctggtgggc ggacgaaatc cgctgacgcg cgaccaaaga cgtgatgatt gatcggg
//
LOCUS       DAORQN010000263         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1549_length_1974_cov_32.380928, whole genome shotgun sequence.
ACCESSION   DAORQN010000263 DAORQN010000000
VERSION     DAORQN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1549_length_1974_cov_32.380928"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PK614_09545"
     CDS             complement(<1..596)
                     /locus_tag="PK614_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB82487.1"
                     /translation="MRTNFSSDSQEHSSTLTMGITDLMTSLAVIFILLFSAYVTKVSE
                     TESQAKVPVPIPAPERKVARTTTDDIKGLLRDHFQRFDLSLDADPTDPNVVRIVVPEA
                     LLNFEFGRGALSSVADRFLADAMPTYAALLCGAMRDQIDSLVIEGHTDDRGTDIYNLK
                     LSQERSLNVMVKGLEVIRDSAPWAYRCFHEKTSASGRGR"
     gene            complement(598..>1974)
                     /locus_tag="PK614_09550"
     CDS             complement(598..>1974)
                     /locus_tag="PK614_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82488.1"
                     /translation="RVLLALANSLGFKPFFIPVSVARKLYLWMGAGSFCSGRSGGTDI
                     MMSIWEFFGQDVALFGQLHSPMLSWLGSVGLIALFLWHAGRLTAAISSVQGCYTRVWP
                     TLTSLTASRKSLQSEWLTVPSLSDVKKPANESGGQSERVDLDDLHMLDKAMRREPRLE
                     QAWLHFRKTFIVERTAWFIEPKVFATRTAAEFFPRDLLNSRLNLAFYHQLPSLITGGG
                     LLLTFLAILIGLSKLHADGSHIVGIQGLINGLAGKFLTSIVGLLCANLFVLLEKSALH
                     RLGTTQQQFVAMVDELFPRRTMEQMLENFTPGAGPSHTPAAVGAVPVDLGERLVGTLS
                     DRLSPTVTALREAVEVLSRREPGGRFAAPDRLAEELSRVMQQTMAAPIQELNQAIHTL
                     AHSVEELKRDQKMEPGAEPRESEFESTFFDDTLPSELEEEAIPDDSMSGLRWFANWRQ
                     GASMKGAA"
BASE COUNT          455 a          615 c          528 g          376 t
ORIGIN      
        1 cgacctcgcc cactggcaga cgtcttttcg tggaagcagc gataggccca gggcgccgag
       61 tcccggatga cttccagccc tttcaccatc acattcaacg aacgctcctg gctcaacttc
      121 aggttgtaga tgtccgtacc gcgatcatcg gtatgccctt cgatcacgag cgaatcgatt
      181 tgatcccgca tggcaccgca gagcaacgcc gcataggtcg gcatcgcatc cgcaagaaat
      241 cggtccgcca ccgacgagag tgcgcccctc ccgaactcaa agttcagcaa cgcctccggc
      301 acgacaatcc gcaccacatt gggatcggtg ggatcggcat ccagggaaag gtcgaatcgt
      361 tgaaaatggt cgcgcaacag ccccttgata tcatccgtcg tcgtccgggc caccttccgc
      421 tccggcgccg gaatggggac cggcaccttc gcctgtgatt ccgtttcaga gactttggtg
      481 acataggcgc tgaacagaag gataaaaata acggcgagtg acgtcatcaa atccgtgatg
      541 cccatggtga gagtggagga atgttcctgc gaatccgacg agaagttggt gcgcatatca
      601 ggccgcgccc ttcattgagg cgccctgacg ccaattcgca aaccaccgta acccgctcat
      661 gctgtcatca gggatggctt cctcctccaa ttctgacggc agcgtgtcat cgaagaacgt
      721 cgattcaaac tcgctctctc tcggctccgc tcctggctcc attttttggt cacgtttgag
      781 ttcctcgacc gagtgggcaa gcgtatgaat cgcctggttc aattcctgaa tcggagccgc
      841 catcgtctgc tgcatcaccc gcgacaactc ctccgccaaa cgatccggtg cagcgaaccg
      901 gccacccggt tcccgcctgc tcaacacctc tacggcctcc ctcagcgccg tcacggtggg
      961 actcaaccga tcactgaggg tacccacgag gcgctcgcca agatcaacgg gcaccgcacc
     1021 cactgcggca ggcgtgtgtg atggccccgc tccgggggtg aagttctcta acatctgttc
     1081 catggttctg cgcgggaaca gttcatcgac catggcgaca aactgctgct gggtggtgcc
     1141 caaccgatgc aaggcggact tctccagaag cacaaagagg ttggcacaga gcaatccgac
     1201 aatcgacgtc aaaaatttcc cggccaatcc gttgatcaac ccttgaatgc ccacgatatg
     1261 tgatccatcc gcatggagtt tgctcaggcc gatgagaatc gcaagaaacg tgagcaacag
     1321 accgcctccc gtaatcaacg acgggagctg atggtaaaag gcgagattca atcggctatt
     1381 cagtagatca cgagggaaaa attccgccgc cgtgcgcgtc gcaaacactt tcggctcgat
     1441 gaaccaggcg gtgcgctcca caataaaggt cttgcgaaaa tgcagccagg cctgttcaag
     1501 gcgaggctcc cgacgcatgg ccttgtcgag catgtgaaga tcatccaaat cgacccgctc
     1561 cgattgacca ccggactcgt tggccggttt cttcacatcg ctcaagctgg ggaccgtcag
     1621 ccactctgat tgcagactct tgcggctggc agtgagactc gtcaaggtcg gccatacgcg
     1681 ggtgtagcac ccctgaacgc tggagatggc cgccgtcagc cgcccggcat gccatagaaa
     1741 cagcgcgatc aatccgacgg aaccgagcca actcaacatg ggactatgga gctgcccgaa
     1801 gagcgccacg tcttggccga agaattccca gatggacatc atgatgtcag tacctcccga
     1861 tcgacccgag cagaacgacc cagcacccat ccatagatac aactttcggg ccacagatac
     1921 cgggatgaag aacggcttga aacccagtga atttgcgagt gcgaggagca ctcg
//
LOCUS       DAORQN010000264         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1552_length_1972_cov_5.811163, whole genome shotgun sequence.
ACCESSION   DAORQN010000264 DAORQN010000000
VERSION     DAORQN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_1972_cov_5.811163"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK614_09555"
     CDS             complement(<1..336)
                     /locus_tag="PK614_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82489.1"
                     /translation="MEAMEILASGMVTGIGLNAQASCAAIRCGITNFAETRFMDKGGE
                     WIIGCSVPLAEPWRGRAKLLRLIVPAIQECLGSVKNISVNEIPLLLCLAEKTRPGRLD
                     GLDQTLLAEI"
     gene            complement(339..554)
                     /locus_tag="PK614_09560"
     CDS             complement(339..554)
                     /locus_tag="PK614_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82490.1"
                     /translation="MDTLMIEPDQRRFMLTWRSSYVLKRNMFEVVQVVAGVMPKGWYR
                     ARELGKTYCPSLGELVAAKMRERSEEE"
     gene            complement(542..1441)
                     /locus_tag="PK614_09565"
     CDS             complement(542..1441)
                     /locus_tag="PK614_09565"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HRB82491.1"
                     /translation="MELLNATKMQAGYTMGMAPDGRESLVVVVKGTFRFPEKGSQEPI
                     LADEQVPLVMADTFAGEPGFSAPIYESEFAPKKPKCDVLLNGSAYAPGGQPTKRVTVT
                     LQVGSWSRSFTVVGNRTWKKGWFGVSATKPEPFTVMPISYGNAFGGTDTTHEDPTKHK
                     FVLANPVGRGFHHQLSAKLINGKPLPNTEEEGRPVKKPGGAYRPMAFGPVGRNWDPRY
                     KLAGTYDQAWIDNVFPFLPPDFQEAYYQAAPLDQQIPYVRGGEVVSLTNLTPEGSTRF
                     TIPTVEMPIVFFPKKGDKASWIR"
     gene            complement(1454..>1972)
                     /locus_tag="PK614_09570"
     CDS             complement(1454..>1972)
                     /locus_tag="PK614_09570"
                     /inference="COORDINATES: protein motif:HMM:NF040117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HRB82492.1"
                     /translation="REMERARTEDVFELLTSASREATEPQRDPKASLNGVLRGVISEF
                     NQATGGIQIVVPGLSMLEPMVARSTVPITQVDVGREVLVAFEAGDARCPYIVGLIWEQ
                     RQNLPAQAESIEAKVDGEQVVIEGKKEIVLKCGKASITLTRAGKVLIRGAYVLSRSSG
                     VNRVKGGSVQIN"
BASE COUNT          394 a          608 c          523 g          447 t
ORIGIN      
        1 gatttcggca agcagcgttt ggtccaagcc gtccaaccgg cctggcctgg ttttttctgc
       61 cagacacaag agcaacggaa tctcgttgac cgaaatattt ttcaccgagc ccaggcattc
      121 ctggatggcc ggcacgatga ggcgcagcag tttggctcga ccgcgccagg gctcggccaa
      181 cgggaccgag cagccgatga tccactcgcc gcccttgtcc ataaaccggg tttcggcaaa
      241 attcgttatg ccgcagcgaa tggccgcgca ggaggcctgc gcatttagac caatacccgt
      301 caccatgcca gaagcgagaa tttccatcgc ttccatactt attcctcttc ggatcgttct
      361 cgcatcttgg ccgcgaccaa ctccccgagc gaggggcagt aggtcttgcc gagttcgcgg
      421 gcacggtacc acccctttgg catgactccc gccacgactt gcaccacctc gaacatgttt
      481 cttttcagaa cataggacga ccgccaggtc agcatgaacc gtcgctgatc cggctcaatc
      541 atcagcgtat ccatgacgcc ttatcacctt tcttgggaaa gaacacgatg ggcatctcca
      601 ctgtcggaat cgtgaagcgg gttgaccctt ctggagtgag attcgtcagc gagacaacct
      661 ctccacctcg tacgtacgga atctgctgat cgaggggcgc ggcttggtag taggcctcct
      721 gaaaatccgg cggcaggaag ggaaagacat tgtcgatcca ggcttgatcg taagtgccgg
      781 cgagcttgta gcgtgggtcc caattgcgtc cgaccggccc aaaggccatc gggcgatagg
      841 ctccgccagg cttcttcact ggacgacctt cttcttcggt gttcggcagg ggcttgccgt
      901 tgatcagctt cgcgcttagt tgatgatgga agccgcgccc tactggattg gccaatacga
      961 acttgtgctt ggttggatcc tcatgggtcg tatcggtccc gccaaacgcg ttcccatacg
     1021 aaatcggcat gacggtaaag ggctccggct tcgtggccga gacgccgaac catcctttct
     1081 tccacgtccg attgcccacg actgtaaatg acctgctcca agagccgact tgcagcgtaa
     1141 ccgtgactcg tttagttggc tgtccgcctg gtgcgtaggc gctgccattc agcagtacat
     1201 cgcacttagg tttcttcggc gcgaattcac tttcgtagat cggcgcagaa aagccaggct
     1261 ctccagcgaa ggtgtcggcc atgacgaggg ggacttgttc gtcggcaagg attggttcct
     1321 gactgccctt ttctggaaac cggaatgttc ccttgaccac gaccaccaga gattcacgcc
     1381 cgtccggcgc catccccatc gtataaccgg cctgcatctt ggtggcgttg agcagttcca
     1441 tagaagatgt gccctagttg atttggaccg acccgccttt gacacggttc acgccggaag
     1501 aacggctaag cacgtaggct cctcgaatga gaacttttcc cgcgcgcgtc agggtgatgc
     1561 tcgccttgcc acacttcaag acaatttctt tcttgccctc gatcacaacc tgttccccat
     1621 ccaccttcgc ttcgatcgac tcagcttgag ccggaagatt ctgtctctgc tcccagatca
     1681 aacccacgat gtatggacat cgtgcgtcgc ccgcctcaaa tgccactagg acttcccgtc
     1741 ccacgtcgac ttgcgtgatc ggcaccgtgg accgtgccac catcggctcc agcatggaga
     1801 ggccgggcac cacgatctgg atacctcccg ttgcctgatt gaactctgaa atcacgcctc
     1861 gtaggactcc attgagggag gctttaggat cacgttgcgg ctccgtcgcc tcacgagatg
     1921 cagaggtcaa gagctcaaat acatcttccg ttctcgctcg ttccatctcc cg
//
LOCUS       DAORQN010000265         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1557_length_1966_cov_6.143904, whole genome shotgun sequence.
ACCESSION   DAORQN010000265 DAORQN010000000
VERSION     DAORQN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_1966_cov_6.143904"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PK614_09575"
     CDS             <1..1469
                     /locus_tag="PK614_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRB82493.1"
                     /translation="VGLLLLQYLFLTRCRGPVVGLFGAAMLLLNLEIIGLSRMALTDS
                     VLIFFTTLSLYGFWLGLYGEGRERHYLWFFYIGMALATLTKGPIGFLIPMLAVGLYLW
                     LTRSWGLFWRHGFPIPGLLLFLLLALPWYLMMLNIHGQRYTTSAQGDTIGRFLGTMEG
                     HGGTLLFYLPVFLLGFFPWSGLLPFAWYQAYRSWRDSKRSGALPPSQTSVLDVHPSPH
                     ALEWFTAAWILGGLVFFSLSSTRLPHYIGPLFPAAAILTASYWNRCVTDQATPGLRAA
                     IHTITAVGCILALAFASLPPLYAKFAGKLIDEFPLAGQVTLGPGPYTVASIFLVGMGL
                     IAYFGFSETRRPAVFWVAGGSLALVVLATTQLIFPLVHHFVIEPPQQLAEVAGVNLGP
                     NDRLILYGQPRPSLVFYAKRKAIVVPKNEEANIKPYLTQPGRTMILLPAALRNRLPFE
                     TMDYPVLLERYGYILLANQSLIHVPEEAEQPPVRIPGH"
     gene            complement(1478..>1966)
                     /locus_tag="PK614_09580"
     CDS             complement(1478..>1966)
                     /locus_tag="PK614_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82494.1"
                     /translation="VLGFVVGSIFNGPLRWWGRSCGQALIRITPVVLSLTGLFWVLTH
                     RIVDPMTDHILLASGAMLVAGGMALRMAGRRNPAGDGHVTRVAAAESLVGLGLGVVTG
                     APVVIGLAGLLCFARWNGRVACADPCSVAPPSSSRHILYLAGVIAAVVVIQSLKGLVP
                     LQ"
BASE COUNT          350 a          657 c          546 g          413 t
ORIGIN      
        1 gtgtcggatt gctcctgctc caatacctct ttctgacccg ctgccggggg ccggtagtgg
       61 gcctcttcgg cgcggccatg ctgttactga acctggaaat catcggcctc agccggatgg
      121 cgctcaccga cagcgtgttg atcttcttca ccaccttgtc gctctacgga ttctggctgg
      181 gtttgtacgg agaaggccgt gaacgccact acctgtggtt cttctacatc ggcatggcgc
      241 tggcgaccct gacgaagggc ccgatcggct tcctgattcc catgctggca gtggggttgt
      301 acctgtggct gacccgctcc tgggggctct tttggcgtca tggatttccc attcccggcc
      361 tcctgctgtt cctgctcctg gcgttgccct ggtacctgat gatgctgaat atccatggac
      421 agcggtatac gacctcggcg cagggcgaca cgatcggccg gttcttgggc accatggaag
      481 ggcacggcgg cacgctgctc ttttacctcc ccgtgttcct gcttggattt tttccctgga
      541 gcggcctgct gcctttcgcc tggtaccagg cctatcgcag ctggcgggac tcgaaacgat
      601 ccggcgccct gcccccgtcg cagacctcgg ttctggatgt ccacccttcg ccgcatgcgc
      661 tcgaatggtt caccgccgcc tggatcctgg gcggattggt ctttttcagc ctgtcctcaa
      721 cccgcttgcc gcattacatc ggtcctctgt ttcctgcggc ggcgatcttg acggcgtcct
      781 attggaaccg ctgcgtgacc gaccaggcca ctcccgggct gcgcgccgcg atccacacca
      841 tcaccgccgt aggctgcatc ctggcgctgg cctttgcttc attgccgccc ctctatgcca
      901 agtttgcagg aaagctgatc gatgaatttc ctctggcggg gcaggtgacg ctcggaccgg
      961 ggccctatac cgtcgcttcg atcttcctgg tgggtatggg cctcatcgcc tacttcggat
     1021 tcagcgagac gagacgaccg gccgtattct gggtcgccgg cggatcgctg gccctcgtcg
     1081 tccttgcgac cacacagttg atcttcccac tggtgcacca ctttgtaatc gaaccgcccc
     1141 agcaactcgc cgaggtcgca ggcgtcaatc tcgggcccaa cgatcggttg atcctctacg
     1201 ggcagccacg cccctccttg gtgttttatg caaagcggaa agccatcgtc gtgccgaaga
     1261 atgaagaagc gaacatcaag ccctatctga ctcaaccagg gcgcacgatg attctcctgc
     1321 cggcggcgct tcgaaacagg ctaccgtttg aaaccatgga ctacccggta ttactcgagc
     1381 gctacggcta catcctgctg gccaaccaat cgctgatcca tgtgccggaa gaggcggagc
     1441 agccgccggt tcgcattcca gggcactgat ccattcctca ctgcaacggc acgagccctt
     1501 tgagtgactg gatcacgacc acagcagcaa tgactcctgc caaataaagg atgtgcctgg
     1561 aggaggaagg cggcgcgacc gagcacggat ccgcgcaggc gactctcccg ttccatcgtg
     1621 cgaagcacaa tagtccggcc aacccgatca cgaccggggc gccggtgacg acacctaatc
     1681 ccaatccgac cagactttct gccgctgcga ctctagtcac atgaccgtcg cccgccggat
     1741 tccgacgtcc tgccattcgc aacgccatac ctccggccac cagcatggct ccggatgcaa
     1801 gcaggatgtg atccgtcatc gggtcaacga tgcgatgcgt cagcacccag aagagacctg
     1861 tcagcgatag gactaccgga gtgatacgga taagcgcctg cccgcaggac cgtccccacc
     1921 agcgcaacgg gccattaaag atcgacccca ccacaaaccc cagcac
//
LOCUS       DAORQN010000266         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1559_length_1963_cov_6.392558, whole genome shotgun sequence.
ACCESSION   DAORQN010000266 DAORQN010000000
VERSION     DAORQN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1559_length_1963_cov_6.392558"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /gene="ruvX"
                     /locus_tag="PK614_09585"
     CDS             <1..202
                     /gene="ruvX"
                     /locus_tag="PK614_09585"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRB82495.1"
                     /translation="RLMKGQRILAIDHGSKRIGFALSDELGWTAQPLETFYRRNPDAE
                     VKDVHTRVELCRQSISYIGASV"
     gene            complement(365..745)
                     /locus_tag="PK614_09590"
     CDS             complement(365..745)
                     /locus_tag="PK614_09590"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB82496.1"
                     /translation="MAPRRYVRTYYRFPLNYPVIFGGAPFVGEGVLTNLSLKGCSVMC
                     DREVLCGSDVRVSVLLDHQPPALPIDLGTIKWVKGHQFGVEFLRLPFEAQQRLNRTLR
                     TELIEWLKSRQNSSDLLETSNPDN"
     gene            complement(932..1363)
                     /locus_tag="PK614_09595"
     CDS             complement(932..1363)
                     /locus_tag="PK614_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB82497.1"
                     /translation="MSRYVVDASMAIKWFIPEVHSEAALRLCRSQIRLHVPAFVTLEL
                     GNVIVKKIRRGELSPAEGETIRGELTQLPLQQHADARLFPAAYQFAIATQRSLYDCLY
                     LALAAAIDGVLVTADRKFYAAVAKGVYGRRVLWVEDLPPAA"
     gene            complement(1360..1593)
                     /locus_tag="PK614_09600"
     CDS             complement(1360..1593)
                     /locus_tag="PK614_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRB82498.1"
                     /translation="MAQVLVRQLNEQVVERLKKRAKEHGRSLQSEVKTILEEAVPDYE
                     GAWLRIEGFRSRLKKSGRKFSDSAGLIREDRDR"
BASE COUNT          454 a          539 c          560 g          410 t
ORIGIN      
        1 tagactgatg aaaggccaac ggattctcgc catcgatcac ggctccaagc ggatcgggtt
       61 tgccctgagt gatgagctcg ggtggacagc ccagccgctg gaaacgtttt accggcgaaa
      121 tcccgatgcc gaggtgaagg atgttcatac gcgtgtcgaa ctatgccgtc agtccatttc
      181 ttacatcggc gcatcggtct gataacttga agcaatgagt cgatcagact ggagacacga
      241 ggatttttgt ctaggcagcg gcacgagcgg ccatcagcgc aaggccgctg ccgcggggca
      301 agagtggttg atttcgttga cgagagacta ggggcgccgc gagaagacgg gaaacgatcg
      361 caggttagtt gtctgggttg gatgtttcca gtaggtcact gctgttctgg cggctcttca
      421 accattcgat gagttcagtg cgaagtgtgc gattgagacg ttgttgggct tcaaagggaa
      481 gacggagaaa ctccacacca aactgatgac ccttcaccca tttgatcgtg ccgagatcga
      541 tggggagtgc cggcggctga tggtcgagga gcacactcac gcgcacgtca ctaccgcaga
      601 ggacttctct gtcgcacatg acggaacaac ccttcagcga caggttcgtc agaacacctt
      661 ctccaacaaa tggtgccccg ccgaagatga ccggatagtt gagcggaaac cggtagtagg
      721 ttcgaacgta ccgacgaggt gccatcagat cctccctgtg ggcggatggt actcaggccg
      781 gccctgcgac cgatatccta ccttgggagg ggaaggggca attaccgacc cgatgactaa
      841 ggagcgaggg aagtcagcgg cctgaaaacc gactttcagg agagcggtgt gagccagaat
      901 tggatacaga gcctgaacac attccgggag gctatgctgc ggggggaaga tcttccaccc
      961 acagcaccct tcgcccataa acgcccttgg ccacagccgc gtagaatttt cgatcagccg
     1021 tcacgaggac gccgtcaatc gccgcagcca aagccaggta caagcaatca tacagacttc
     1081 gttgcgtggc tatcgcgaac tgatacgcgg caggaaacaa tcgtgcatcg gcatgctgct
     1141 gaagcggaag ttgcgtcaac tctcctctaa tcgtttcgcc ttcggccggc gagagctccc
     1201 cgcgccggat tttcttcacg atgacgttgc ccagttccaa cgtcacgaat gccggcacat
     1261 gcagtcgaat ctgtgagcga cataaccgaa gtgccgcttc cgagtggact tccggaataa
     1321 accatttgat tgccatgctg gcatcaacga cgtatcgact caccgatcac gatcctcacg
     1381 aataagcccc gcgctgtcac tgaattttcg accggacttt tttaatcggc tgcggaaccc
     1441 ttctatccgc aaccaagcgc cttcgtagtc gggaaccgct tcctccaaaa tcgttttcac
     1501 ctctgactgc aaagaccgcc catgctcttt tgctcgcttc tttaaccgtt ccaccacctg
     1561 ctcattgagc tgtcgtacaa ggacctgcgc catcgggacc tcccaaattc agagcattga
     1621 tagcagaacg atagcaggcg cgtatcccat ggtcaatagg aggccgcatg aacggcagct
     1681 gagcgttctt cgtcgagcaa acctatcgcc cctccttgtt ttgccggcga gatctgagta
     1741 ggcttcgggc ctcaaactgg aatcacaata agggctttgg ggtagaaaga agggctgtat
     1801 gcagaggtat cgacaggttg tgatctggat ggccggggcc ttgctggtgc agggatgcgt
     1861 cggtttcgga gcctggacgc tggggagtcg tactgaatcc agcgaccatc caaaaattga
     1921 tcgaacgaga ggtgtgctcg acgtgcgcaa cggcgaaccc caa
//
LOCUS       DAORQN010000267         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1562_length_1960_cov_4.447769, whole genome shotgun sequence.
ACCESSION   DAORQN010000267 DAORQN010000000
VERSION     DAORQN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_1960_cov_4.447769"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK614_09605"
     CDS             complement(<1..236)
                     /locus_tag="PK614_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HRB82499.1"
                     /translation="MTPSIKTLQFDLDSEKQRAALKATEYVQDGMVVGLGTGTTSKHL
                     IIALGERVRAGLKIQAVPTSHDTAALARQSGIPL"
     gene            complement(233..946)
                     /locus_tag="PK614_09610"
     CDS             complement(233..946)
                     /locus_tag="PK614_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRB82500.1"
                     /translation="MRLLNDLEATAHGLLLLNPDEIVVLNAGAPPKKKQALALIAAGT
                     GLGECILYWDGGRYRPMPSEGGHTVFAPNSDSEIDLLRHLRGSYLHVSYERIVSGPGL
                     HAIYEYLRDTKKNEPTWLAEKIKAGNPAAEIAEAGLKGQAEIAKQALDLFESIYGSEA
                     GKLALKALTLDGVYVAGGIAPKLLKKLQDGSFMRGFTNKGRYKRIMSQIPVKVVMNDK
                     TALLGAASMAAQLTSSTSS"
     gene            complement(984..1322)
                     /locus_tag="PK614_09615"
     CDS             complement(984..1322)
                     /locus_tag="PK614_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRB82501.1"
                     /translation="MILAGDIGGTKTNLALYDWTTERVEPVREDSFHSADYKTLEEII
                     EEFLSAPLPKPPAEDELGDEPVEAPAEGTTEAPELPPEPIKLTAACFGVAGPVIDNRC
                     RTTNLPWLVH"
     gene            complement(1340..>1960)
                     /gene="pgl"
                     /locus_tag="PK614_09620"
     CDS             complement(1340..>1960)
                     /gene="pgl"
                     /locus_tag="PK614_09620"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRB82502.1"
                     /translation="PKALYSILTSDKYAQQLDWSKVHFLFGDERSVPPTHADSNFAMA
                     DAILFSPLHIPSAQIHRMRGEDPPETAAAQYETTLRHLTAAVPGQWPRLDLVLLGMGD
                     DGHTASLFPGTASLTEQTRWVVPSTSPQGTRARVTLTLGVINHASVVLFLVAGRSKAA
                     VVRRVLEQRPGDPGPYPAALIRPETGRLLWYLDRAAASELTATTDD"
BASE COUNT          343 a          553 c          620 g          444 t
ORIGIN      
        1 atgaggggaa tgccggattg cctggccaga gccgctgtgt cgtgcgaggt cggcacggct
       61 tggattttga gccctgcacg gacgcgctcg ccgagggcga tgatcaaatg cttcgatgtc
      121 gtaccggtcc ccaacccgac gaccatgccg tcttgcacat attcagtggc tttcagcgcc
      181 gcccgctgct tttcggaatc gagatcaaat tgtagtgtct tgatggatgg ggtcatgatg
      241 aggtcgatga tgtgagttgt gccgccatcg aggcggcgcc gagcaaggcg gttttgtcgt
      301 tcatcacgac cttcaccgga atctggctca tgatccgttt gtaacggccc ttgtttgtga
      361 acccgcgcat gaaggagccg tcctgcagct tcttcagcaa tttgggcgca atcccgccgg
      421 cgacatagac tccgtcgagt gtgagcgcct tgagcgccag tttgccggct tccgagccat
      481 aaatggactc aaataaatcc agggcttgct tggcgatttc ggcctgtccc ttgagtccgg
      541 cctcggcaat ctcggcggca ggattaccgg ctttgatctt ctccgccaac caggtcggct
      601 catttttttt ggtgtcgcgg agatattcgt aaatggcatg gaggcccggc ccggatacga
      661 tgcgttcgta actgacatgc aggtagctgc cgcgcaggtg gcgaagcaag tcgatttcgc
      721 tgtcgctgtt gggggcaaag accgtgtgcc cgccttccga cggcatgggg cggtagcgac
      781 ctccatccca atagagaatg cattcaccga gcccggtacc ggctgcgatc aaggcgaggg
      841 cctgtttttt cttcggcggg gcgccggcgt tcagcactac gatttcatcg ggattcaaga
      901 ggaggagccc gtgggccgtt gcttccaggt cgttgagcag gcgcacctgg gggatggcga
      961 atcgttccgc gagtgtcgcc ccatcaatgc accaaccagg gaagattggt ggtgcggcaa
     1021 cggttatcga tcaccggccc tgccacgccg aagcacgccg ccgttaattt gatgggctct
     1081 gggggaagct ccggtgcctc cgtggttcct tctgccggcg cctcgacggg ctcgtctccc
     1141 agctcgtctt ctgctggtgg cttgggcaac ggtgcactga gaaattcttc gatgatttct
     1201 tcgagggtct tatagtccgc gctatgaaag ctatcctccc gcaccggctc gacccgttca
     1261 gtcgtccagt cgtagagcgc tagattggtt tttgtgcctc cgatatcgcc tgccaggatc
     1321 atggtgcctc ttgcgatgac taatcgtcag tggttgcggt caattcggat gcggcggcgc
     1381 ggtcaagata ccacaacagt cgtccggtct caggccgaat caaagctgcc ggatacgggc
     1441 cgggatcacc gggccgctgc tccagtacgc gtcgcacaac cgcggctttg ctccttccgg
     1501 cgacgaggaa cagtaccaca ctcgcgtgat tgatcacacc tagggtgaga gtcacccgtg
     1561 cccgcgttcc ttggggagac gtgctgggaa ctacccaacg agtctgttcg gtcagtgatg
     1621 ccgtgccggg gaagagtgag gcggtgtggc catcgtcgcc catgcccaga aggacaaggt
     1681 ccaaccgcgg ccattgcccg gggacggctg cggtgaggtg acgcagggtg gtttcgtatt
     1741 gagctgccgc ggtttcaggc gggtcttcgc cgcgcatcct gtgaatctgc gctgagggaa
     1801 tgtgcaacgg gctgaacaat atggcgtccg ccatggcgaa attgctgtcg gcatgcgtag
     1861 gcggtacgct gcgctcgtct ccgaacagaa agtgcacctt gctccagtcg agttgctgtg
     1921 cgtatttgtc gctggtcagg atcgaataca gtgctttcgg
//
LOCUS       DAORQN010000268         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1563_length_1959_cov_4.228992, whole genome shotgun sequence.
ACCESSION   DAORQN010000268 DAORQN010000000
VERSION     DAORQN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1563_length_1959_cov_4.228992"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK614_09625"
     CDS             complement(<1..253)
                     /locus_tag="PK614_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRB82503.1"
                     /translation="MQSATTGFSTVLDAPGNVVATKELAEIMGPKAFAFPKPSQLIGL
                     LIEQATSPGDLILDSFAGSGTTGHAALTLKTPFRIRSRVA"
     assembly_gap    317..351
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<352..1134)
                     /locus_tag="PK614_09630"
     CDS             complement(<352..1134)
                     /locus_tag="PK614_09630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRB82504.1"
                     /translation="MPTLNWIGKEAVVNHHRQVPFHLLKDVPDLACGEPGEGNLIVQG
                     DNLVALKALLPYYAGQVKCIYIDPPYNTGNENWVYNDNVNSPLMREWLGKVVGKEGET
                     LDRHDRWLCMMYPRLALLRQFLRQDGVILISIDDHEYERLKLTMNETFGAENHVATFI
                     WKRRTATDSRNLNGISGDHEYILCYQRTAGFRVQGQAKDLGKYTNSDNDSRGPWMSDN
                     LTGLANPTERPNLHYEIVNPKTGISYQPHPSRGWFYGRERMAG"
     gene            complement(1271..1591)
                     /locus_tag="PK614_09635"
     CDS             complement(1271..1591)
                     /locus_tag="PK614_09635"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB82505.1"
                     /translation="MGEKIGIELPKQQIAAFCEKWRIRQLALFGAVLRRDFRPDSDVD
                     VLVTFAPQADWKFEHLLEMKGELESLIGRTVDLVEKRVIDESPNYIRRKHILTHMEPI
                     YVAG"
BASE COUNT          395 a          567 c          499 g          463 t
ORIGIN      
        1 aagcgacgcg gctccgtatc ctgaacggcg ttttcaacgt caatgcggca tgtcctgtcg
       61 tcccggaacc agcgaaggag tcaagaatta gatcaccagg gcttgttgcc tgttcgataa
      121 gaaggccaat gagttgcgac ggttttggaa aggcaaaggc cttcggcccc attatctctg
      181 cgagttcctt cgttgccaca acattgcctg gtgcatctaa taccgtcgaa aaccctgtcg
      241 ttgcagattg catatccgat atgaaccgct taagcctggg acgtccctcc cgtttccgtg
      301 gccataagat tcggttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcctgccat
      361 acgctcccgc ccgtaaaacc atcctcgtga tgggtgaggt tgatagctta tgcccgtctt
      421 cgggttgacg atttcatagt gaagattggg gcgctcggtg ggattcgcta acccggttaa
      481 gttgtcgctc atccaaggac cgcgcgaatc attgtcggaa ttcgtgtact tgccaaggtc
      541 cttggcttga ccttgcacac ggaaccctgc agtgcgttgg tagcacagaa tatattcgtg
      601 atcgcctgaa atgccattta ggtttcggct gtccgtggcc gttcgccgct tccaaatgaa
      661 tgttgcaacg tgattttcag cgccaaaagt ttcgttcatt gtcagcttta ggcgttcata
      721 ttcatgatca tcgatgctta ttagaatcac accatcttgc cgcagaaact gccgcagcag
      781 cgcgagcctc ggatacatca tgcagagcca gcggtcgtgc cggtcgagtg tctcgccctc
      841 cttgccgaca accttgccga gccattcccg catcagagga ctgttcacat tgtcgttgta
      901 gacccaattc tcgttgcccg tgttgtacgg cgggtcgatg tagatgcact tcacctggcc
      961 cgcgtaatag gggagtaacg cttttagggc taccaggttg tcaccctgca caataaggtt
     1021 gccctcaccc ggctcgccgc acgcgagatc ggggacatcc ttcagcaagt gaaacggcac
     1081 ctgccggtgg tggttgacca ccgcttcttt cccgatccaa ttcaacgtgg gcatgcaaca
     1141 atcctctgag cctgagccgc tgagggatac ccgttccagg aagggaaatc aagctgacgg
     1201 cgagcggctt aggaagggcg cggttacgag gcgagtcagg cctcggactg gtcaggcggg
     1261 aatatgtggc ttatcccgcc acatagatgg gttccatgtg ggtcaaaata tgtttgcgtc
     1321 gtatgtagtt cggactttcg tcgatcactc gtttctcgac caaatccacc gtccgcccga
     1381 taagggattc gagttctccc ttcatctcga gcaaatgctc gaatttccag tcggcctgcg
     1441 gagcaaacgt gaccaacacg tccacatcgc tatccggcct gaaatccctc cgcagcaccg
     1501 caccgaagag ggccagctgg cgaatgcgcc atttctcaca aaacgcagca atctgctgtt
     1561 tcggcagttc aatgcctatc ttctcaccca tagcagcact tctcctgctc acaccaattg
     1621 agggataccc ttttcccgac aggaaatcaa tcgacagcct cccaaatatc tttagtcatg
     1681 ctccttccaa gctcgcccgt actcttctct ccccaggctg gcgtccggcg atcctcaact
     1741 gcgcgcgtcg aacgaggccc ttcatagggc gcgcgttccg cgaggtgcgc gacgcgagga
     1801 ataacgagcg tcacggttgc acacgccgcc cctgcccctc tccaaacatg gtgatgatcc
     1861 ttccaagctt gcttgtttcc ccttcaggga gggtggtttg gttggtctcc cactgcgcgc
     1921 catcgaccga gcacattctg atcgtgcgcg gtctgcgag
//
LOCUS       DAORQN010000269         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1583_length_1936_cov_7.748006, whole genome shotgun sequence.
ACCESSION   DAORQN010000269 DAORQN010000000
VERSION     DAORQN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_1936_cov_7.748006"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK614_09640"
     CDS             <1..191
                     /locus_tag="PK614_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82506.1"
                     /translation="RADVTITNGQALTFLRLPPCDVSASEIRTRLRSGASLANLLPAP
                     VESYILRQGLYREDSERL"
     gene            178..600
                     /gene="rsfS"
                     /locus_tag="PK614_09645"
     CDS             178..600
                     /gene="rsfS"
                     /locus_tag="PK614_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRB82507.1"
                     /translation="MSVSESKAKALAVASAILDKKATDVLILHIATLTSVADYLVIGS
                     GESERQARAMADHVGDVLTAQGHRPLSTEGASSAKWVVMDFGDVIVHIFQKDIREHYA
                     LERLWGDAGQVRLPEERAVKAAPPARTATRPARTRKRV"
     gene            602..>1936
                     /locus_tag="PK614_09650"
     CDS             602..>1936
                     /locus_tag="PK614_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB82508.1"
                     /translation="MFRLLSTIFLVSVGIFLYSYFRELNPGFITVRTSPDALFELSPV
                     SLVLFSMALGATLVALIVTIKETSHVFMNWRTNRLVRRKEKVDALHRDGTHAFMSKRT
                     ADAVNLFERALVIDPNRTDSLLWLGNIYRSESNFTEAIRLHQQAHRIEERNVEVLLEL
                     AKDLEGARRYEDALQTLQNILRIEPDNLMALIRKRDLQIRLEKWSDALEIQHRLVKAN
                     LPESERRAESNLLLGCMYEVGRQLLERGHPDKARRYFRGAIKKDRTFLPAYIGIGEIL
                     VREGKTKNAVEILKKIYAKTRSIIILHRLEELFLELGEPDEIIRVYQEALQQDPHNAV
                     IQFYLGKLYYRLEMVDEAYDVLATLDGTQEQLVDYHKIMANLYLRKQHMDEAVGELKK
                     ALGFKKRVVVPYQCTQCHHELAEWSGRCRRCGRWNTYVALPWRDANQPDTDQD"
BASE COUNT          436 a          596 c          542 g          362 t
ORIGIN      
        1 aacgcgcgga tgtgaccatc acaaacgggc aggctctgac attcctgcgg ttgcccccct
       61 gcgacgtgtc cgcctcggag atcaggacac ggctgcgcag cggcgcctct ctggcaaatc
      121 tgttgcccgc ccctgtcgaa tcctatatac ttcgccaggg gttatacagg gaggacagtg
      181 agcgtctctg aatcaaaggc caaagctctc gcggtggcaa gcgccatcct ggacaaaaaa
      241 gccaccgatg ttctcatcct gcatatcgcc acactgacct ccgtcgccga ctatctggtc
      301 atcggttccg gggaatcgga gcgtcaagcg cgggcaatgg cggaccacgt gggcgacgtg
      361 ctcacggcgc aaggtcaccg gccgctcagc actgaaggtg catcgtcagc caagtgggtc
      421 gtcatggact ttggtgacgt gatcgtccac attttccaaa aggatattcg cgaacactac
      481 gccctcgaac gactgtgggg cgacgcggga caagttcgat tgccggaaga gcgggccgtg
      541 aaagccgccc ccccggcacg aaccgccaca cgtccggcgc gcacccggaa acgggtctag
      601 catgtttcgg ctcctctcca cgatcttcct cgtcagcgtc ggcatctttc tctacagcta
      661 ttttcgcgaa ctcaaccccg gcttcattac ggttcggacc agtccggacg cgttgtttga
      721 attgagcccc gtctcattag tcctcttctc gatggccctg ggcgccacat tggtggctct
      781 catcgtcacg atcaaagaaa cctctcatgt gttcatgaat tggcgcacca atcgcctcgt
      841 ccgccgcaaa gagaaggtcg atgccctgca tcgggacggg acgcacgcgt tcatgtccaa
      901 gcggacggcc gatgccgtga acctgtttga acgtgcgctg gtgatcgatc cgaatcgaac
      961 ggattcgctg ctctggctgg ggaacattta ccgctccgag agcaacttca cggaagccat
     1021 ccgcctccac cagcaggccc accgcattga agaacgcaat gtggaagtcc tgctcgaatt
     1081 ggccaaggac ctggaaggcg cccgacgata cgaggacgcg ctccaaacgc ttcagaacat
     1141 tctccgcatt gagccggata acttgatggc cttgatccgt aaacgtgatc tgcagatccg
     1201 actggagaaa tggagcgatg cgctcgagat ccaacaccgg ctggtcaagg ccaacctccc
     1261 cgaaagcgaa cggcgcgccg aatcgaatct gctcctcggc tgtatgtatg aagtcgggcg
     1321 gcaactcctg gagcgcggac acccggacaa ggcccgacgc tacttccggg gcgcgatcaa
     1381 gaaagatcgg acgttcctgc ctgcctacat cgggatcgga gagattctgg tccgtgaagg
     1441 aaaaaccaag aacgctgtcg aaattctcaa gaagatctat gccaagacac gcagcatcat
     1501 catcctgcac cgactggagg aactctttct agagctgggc gagccggacg agatcatccg
     1561 tgtctaccag gaggctctcc agcaggaccc ccacaatgcc gtgatccagt tctatctcgg
     1621 gaaactctac tatcgactgg aaatggtcga tgaagcctat gacgtgctcg ccacactcga
     1681 cggaacgcag gagcaactcg tcgactacca taagatcatg gccaacctgt acctgcgcaa
     1741 gcagcacatg gacgaggcag tgggagagct caagaaggcc ttgggcttca agaaacgggt
     1801 cgtggtgccc taccaatgta cccagtgcca ccatgaactg gccgaatggt caggtcgttg
     1861 ccggcgatgc ggacgatgga acacctacgt cgcccttccc tggcgggacg cgaaccagcc
     1921 cgataccgac caggac
//
LOCUS       DAORQN010000270         1935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1584_length_1935_cov_4.890426, whole genome shotgun sequence.
ACCESSION   DAORQN010000270 DAORQN010000000
VERSION     DAORQN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1584_length_1935_cov_4.890426"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK614_09655"
     CDS             complement(<1..307)
                     /locus_tag="PK614_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRB82509.1"
                     /translation="MSVGALTKKILQAALFYEIWDAMKVTFNHMFHRPITFQYPREQR
                     TIPDGHRGALGLLRYGDGRDRCVGCDLCEAACPSRCIKVISQEDPERPLQRFASEFYI
                     "
     gene            complement(352..>1935)
                     /locus_tag="PK614_09660"
     CDS             complement(352..>1935)
                     /locus_tag="PK614_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB82510.1"
                     /translation="VHAVRHAVGVGRPLNDWEDLTKAKAILVIGSNITETNPLTAVRI
                     KEAIRVYHSQVIVVDSANTNIAQLASHPLLVKPGSESFLIDGLVKSVIEQDLIDEAST
                     TRHPQAFSALKQALAQVSIEQVSAQTGIAVEQIREIATIFAEAPRAIALCAEGIVRQP
                     NGYQNVLKLIDLAWVTGKLVQPGFGVTTVTEEINEQGAVDMGVAPEFLPGQARFDDPG
                     ARERFEKAWDVTLPASGSGANFLEILARIRNGQIKALYVIGENPLATLPASMDVKGTL
                     EKLQLLIVQDPFLTETGRMAHVVLPAATYAEKDGTFTNLEGKVLRVRQAVDQVGESLP
                     DWHIMTALANGLGYDWTYESPQDVQNEIMKLLPGYYNLGQPRRVSPSADAYLSNGYAA
                     EVTSRYHSQTAQSTLKDNTQPFTLLTGQVLYHSGKMSTRASGLINIEPNNGRIRMNPA
                     DVERLGLNDQSTIRLTSRQGSVQAGVKADPDIQPGSCFFPEHFNEPAVKDLMTVEVDA
                     VTGVPYFKSTRVKIEKVGA"
BASE COUNT          390 a          537 c          563 g          445 t
ORIGIN      
        1 cgatgtagaa ctcgctggca aagcgctgca agggacgttc tgggtcttcc tggctgatga
       61 ctttgataca acgggaggga catgccgctt cgcaaaggtc gcaacccacg cagcggtccc
      121 ggccgtctcc ataacgaagc agccctaatg cgccgcggtg accgtcgggg atggtgcgtt
      181 gttcgcgcgg gtattggaag gtgatggggc gatgaaacat atggttgaag gtcaccttca
      241 tggcgtccca aatttcgtag aacaacgccg cctgaagaat tttcttggtc aaggccccga
      301 cactcattcc caccacctct gtcgacatca aaaagacgag cgagcttact cttacgcacc
      361 aaccttttca atctttacac gcgtggattt gaagtacggc actcccgtca ctgcatccac
      421 ctccacggtc attaggtcct tcacggccgg ctcattaaaa tgctcgggga agaagcacga
      481 tccgggttgg atatcgggat ctgcttttac gccggcttgt acggaacctt gccgcgaggt
      541 gaggcgaatg gtggattgat cgttcagtcc gagccgttcc acatcggctg gattcattcg
      601 tatacgcccg ttgttcggtt cgatattgat gagccccgat gcacgcgtgg acattttccc
      661 ggagtgatag agcacctgtc cggtcagtaa ggtaaagggc tgcgtgttgt ccttcaacgt
      721 cgattgtgcc gtctgactgt gataccggct cgtaacttca gccgcgtagc cgttcgacaa
      781 ataggcatcg gccgatggcg agacgcggcg aggctggccg agattgtagt agccaggtaa
      841 cagcttcatg atttcattct gcacgtcttg cggcgattca taggtccagt catatcccaa
      901 accgttggcc agcgccgtca tgatatgcca gtccggcagg ctttccccga cctgatccac
      961 tgcctgacgc acacggagca ctttgccctc cagattggtg aaggtgccgt ccttttccgc
     1021 gtaggtggca gccgggagga cgacatgcgc cattcgccct gtctctgtga ggaaggggtc
     1081 ttgaacgatg agcagttgga gcttctcgag cgtccccttc acatccatgg aggcgggcaa
     1141 agtggccagg ggattctctc cgatgacata caacgccttg atctggccgt ttcgaatacg
     1201 ggcgagaatt tccaggaaat tagcgccgct tccgctggca ggaagggtca cgtcccaagc
     1261 tttctcgaag cgttcccttg cccctggatc atcgaatcgt gcctgcccgg ggaggaattc
     1321 cggcgcgacg cccatatcca ctgcaccctg ctcattgatt tcttctgtga ccgtggttac
     1381 gccgaatccg ggttgaacta acttgcctgt cacccaggcg agatcaatca gcttgagaac
     1441 gttctggtaa ccgttcggct gccggacgat gccttcggca cataacgcaa tggcgcgcgg
     1501 ggcttcggca aaaatcgtgg cgatttcccg gatctgctcg actgcgatgc cggtctgtgc
     1561 ggagacctgt tcgatggaga cctgcgccag ggcttgcttc aatgcggaaa aggcctgcgg
     1621 atgccgagtg gtgctggctt catcgatcag gtcctgttcg atcaccgatt tcaccagccc
     1681 gtcgatcaag aacgattcgc tgcctggctt gaccaaaagc ggatgcgaag ctagttgcgc
     1741 gatgttggta ttggcagagt cgacgacgat gacctgggag tgatacacgc ggatggcttc
     1801 cttgatgcgg acagccgtca gcgggttagt ttccgtgata ttcgagccga tgaccaagat
     1861 ggctttggct ttggtcaaat cttcccagtc gttgagcgga cggccgacgc cgacggcatg
     1921 acgtacggca tgaac
//
LOCUS       DAORQN010000271         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1594_length_1916_cov_3.616335, whole genome shotgun sequence.
ACCESSION   DAORQN010000271 DAORQN010000000
VERSION     DAORQN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_1916_cov_3.616335"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK614_09665"
     CDS             complement(<1..251)
                     /locus_tag="PK614_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB82511.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGMGVTLAANGSEALALLSKD
                     RPDLIVSDVIMPDKSGYEVCAYIRGQANLADLPVLLISG"
     gene            complement(280..756)
                     /locus_tag="PK614_09670"
     CDS             complement(280..756)
                     /locus_tag="PK614_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRB82512.1"
                     /translation="MLRTQLGRQPHKGSKGATEARMQHMVVFSVGGQRLAARTEEIGG
                     VMPWTGSTAVPSDTPFVRALVRQEEGCLPVFDLAAKFKRSLQDGEPLCLVVKHVDGPL
                     AICIDSQVPSLHMVARSAVQYRAGNDPDIAGTCIAGEEELPIINLTTLGVSSNRPA"
     gene            complement(773..>1098)
                     /locus_tag="PK614_09675"
     CDS             complement(773..>1098)
                     /locus_tag="PK614_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82513.1"
                     /translation="ANEQRSRSFAVDKVVGLIDVERTLIQPLPAQFRGGERERLLGLF
                     VESSYIALIANPFWVLELPSRTNVLDVFALRVSERRPGEFDSRLRLPSAALEEASAMS
                     AGSAK"
     assembly_gap    1099..1112
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1113..1343)
                     /locus_tag="PK614_09680"
     CDS             complement(<1113..1343)
                     /locus_tag="PK614_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82514.1"
                     /translation="MGLRGHKTAVVSSGLAVRFLVALMGPTHVAFPSHWVRGIVTPAE
                     GGQDGHVTWANASYERTDLARRLTIQAKGVTAE"
     gene            complement(1391..>1916)
                     /locus_tag="PK614_09685"
     CDS             complement(1391..>1916)
                     /locus_tag="PK614_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB82515.1"
                     /translation="GRVVLVLDVSRLTTREYHEAMSVIQNPASSPMLEGPAQPASPQL
                     PDTQLPLLLIDDSLSIRKFVGRMLESAGYIVETATDGEEGCRKALVQQYQLIITDLEM
                     PKLNGFEVIQALRARPQTQTTPILVMTTRAGEKHRQMAVSVGASGYIAKPVEERALIQ
                     EVRKWTGRESVVKK"
BASE COUNT          388 a          622 c          528 g          364 t
ORIGIN      
        1 ataccgctga tcagcagcac gggaagatcc gcgaggtttg cctgtccccg aatgtacgca
       61 cagacctcat agccgctctt gtccggcatg atgacatcgg acacgatgag gtcggggcga
      121 tccttactta acagcgccag agcctccgat ccgttcgccg caagggtcac ccccatgccg
      181 gcctcggtca ggagacgctc ggccacctta cggactgcga tactgtcgtc ggcaatcaag
      241 atctttggca tgtcgttcct ctacacaagg caaggctcat cacgcgggac ggttcgatga
      301 gacccccaag gttgtcaggt tgatgatagg aagttcttct tcgccggcga tgcacgttcc
      361 cgcgatgtcg ggatcgttgc cggcgcgata ctgcactgcc gagcgtgcca ccatgtgcaa
      421 ggaaggaacc tgggaatcga tacagattgc cagcggtcca tccacatgtt tgacaaccag
      481 acacagcggt tcaccatcct gcagggaacg cttgaacttc gccgccaggt cgaataccgg
      541 aagacatccc tcctcctgtc ggacgagagc gcgcacaaac ggcgtatcgc tgggaaccgc
      601 cgtcgagccc gtccacggca tcaccccgcc gatctcctcg gttctggcgg ccaaccgttg
      661 cccgcccacc gaaaacacca ccatgtgctg catccgcgcc tcggtcgcgc ctttgctacc
      721 cttgtgcggt tgtctgccta gctgagtacg tagcatggtc gatccctgtg acctacttgg
      781 ccgatccggc tgacatcgcc gacgcctctt ccaaggccgc cgaaggcagg cggagtctgg
      841 aatcgaattc gcccggccgg cgctccgaca ctcgaagggc aaacacatcc aagacattgg
      901 tccgagaggg gagctcgagc acccaaaacg gattggcgat cagcgcgata tacgacgact
      961 caacaaacaa tccgagcaat cgctcccgct cccctccccg aaactgcgca gggagcggct
     1021 ggatcagcgt ccgttcgaca tcgatgaggc ccacgacctt gtccaccgca aaggaccgcg
     1081 atcgttgctc attggcatnn nnnnnnnnnn nnctcagccg tgacgccttt ggcctggatc
     1141 gtcaaacgac gggcgagatc ggtgcgttcg taggaagcat tagcccaggt gacatgcccg
     1201 tcctggcccc cctcagccgg agtcacaatg ccgcgaaccc agtgggaggg aaaggccaca
     1261 tgcgtggggc ccatgagcgc cacgagaaat cggaccgcca acccgctcga cacgactgct
     1321 gtcttatgtc cccggagccc catgcctgtc cttcattctg ccagtaacgg tacgaatcga
     1381 caaaacgttc ctattttttc acgacgcttt cgcggcctgt ccacttgcgg acttcctgga
     1441 tcaaggcgcg ttcttccacc ggcttggcaa tgtatcccga ggcccccaca ctcacggcca
     1501 tctggcgatg cttttcaccc gcccgcgtgg tcatgacgag aatcggtgtg gtctgagtct
     1561 gcggcctggc gcgaagcgcc tgaatcactt caaagccgtt caacttcggc atttcgagat
     1621 cggtgatgat gagctgatac tgctgcacca atgccttgcg acagccttct tccccgtcgg
     1681 tcgccgtttc gacgatatac ccggccgact ccagcatgcg tccgacaaac tttctgatgc
     1741 tgagcgagtc atcgatcaac aagagcggaa gctgcgtatc cggaagctgc ggcgaagcgg
     1801 gctgcgccgg tccctcaagc atcggcgagg aggcggggtt ttgaatgacc gacatggcct
     1861 catggtattc gcgagtcgtc aaccggctga cgtccagaac cagcaccaca cgcccc
//
LOCUS       DAORQN010000272         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1597_length_1913_cov_6.047901, whole genome shotgun sequence.
ACCESSION   DAORQN010000272 DAORQN010000000
VERSION     DAORQN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_1913_cov_6.047901"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PK614_09690"
     CDS             complement(<1..841)
                     /locus_tag="PK614_09690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB82516.1"
                     /translation="MSIDPTNFILLDAAVPEQRARWEDLWDAWPHGDVVAHPGYVQLF
                     ARPEDRGVCACQVGRSGGILFPLIVRPLCVEPWAGGETESCDLVSPYGYGGPFGWGSF
                     DVDAFWEGFDQWARAMHAVSLFARLSLFKDQLIPFYGETIVKGPCVIVSLKQDPDDIF
                     RGYEKAARENIRQAKRAGVTVEPDPDCRRLEEFLAVYSATMNRLGALPIYYFPRSFFD
                     RLIAELAGHVVLFHALHHQRVLSTELLLISEHYLYSFLGGTLEEGFPLRANPLLRHQI
                     NQWA"
     gene            complement(850..1740)
                     /locus_tag="PK614_09695"
     CDS             complement(850..1740)
                     /locus_tag="PK614_09695"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRB82517.1"
                     /translation="MDLSQYEAPLPSDEDVAFYRQHGWYRSTRVLPEALLNHAFSGIQ
                     RHFSGERDWDLPPTSGFSDWKPGDGNIVRTAEVVALQNSQVRQLALHPVLGAIAARLT
                     GSSTIRYFADTLVDKPGILPETESVVGWHTDRAYWGTCTSDSMLTMWIPFQDCTVEMG
                     PLLYIDGSHTWSGTEDMRTFRCKDLRGLEKQFPDKLPMTKIPMTLRRGELSVHHCRLI
                     HGSGPNTSGTPRLALAVHLQDAHNRYRRYNNEKGIPWHIFLDDLASKLKDGSPDYSDP
                     TIFPLLWEQPSVGSATSHNF"
BASE COUNT          443 a          542 c          548 g          380 t
ORIGIN      
        1 tggcccactg gtttatctgg tgtcggagca gcggattggc gcgaagcggg aatccctcct
       61 cgagggttcc gcccaggaac gagtagaggt agtgctcaga gatcaacaac agttctgtcg
      121 acagtacacg ttgatggtgg agtgcatgga acagcacgac atgaccggcg agttcggcga
      181 ttagcctgtc aaaaaacgat ctcgggaagt aatagatggg caacgccccg agacggttca
      241 tagtggcaga gtagacggct aggaattcct ccaagcgccg gcagtctggg tccggctcca
      301 cagtaactcc ggcacgtttc gcctgcctga tattttcgcg ggcagctttt tcatagcccc
      361 ggaaaatgtc gtcggggtcc tgtttgagcg agacaataac acaggggcct ttgacgatcg
      421 tctccccata aaatggaatg agctgatctt tgaacagtga caaccgtgcg aacagggaga
      481 ccgcgtgcat ggcgcgagcc cattgatcga aaccttccca aaaggcatcg acatcgaagg
      541 aaccccaccc gaatggtccg ccatacccat agggcgacac caaatcgcag ctctccgtct
      601 cccctcctgc ccacggctcg acgcacagtg ggcgaacgat caaagggaaa agaattccgc
      661 ccgagcgtcc cacctggcag gcgcataccc ctcgatcctc aggtcgggca aagagttgga
      721 catatcctgg atgggcgacc acatccccat gcggccaagc atcccagaga tcctcccagc
      781 gggcccgctg ctccggcaca gcggcatcaa gcagaatgaa gtttgttgga tcgatactca
      841 tagcgacgct cagaagttgt ggctggttgc tgagcctaca gatggttgct cccagagaag
      901 ggggaagatt gtcggatctg aataatcggg ggatccgtct ttaagttttg aggccaaatc
      961 gtcgagaaaa atatgccagg gaatgccctt ctcattattg tatcggcgat agcgattgtg
     1021 tgcgtcctgc aaatgtacgg caagggcgag gcgcggagtt ccgctggtgt tcggcccgct
     1081 gccgtgaatc aagcgacaat gatgcacgct gagttcccct cgacggagag tcatgggaat
     1141 cttggtcatt ggaagtttat caggaaattg tttttccaat ccgcgaagat ccttacaacg
     1201 aaacgtgcgc atgtcttcgg tccctgacca cgtatggcta ccgtcgatat acaaaagcgg
     1261 gcccatttca accgtgcagt cctggaacgg aatccacatc gtgagcatgc tgtctgaggt
     1321 gcaggtgccc caataggctc ggtcggtgtg ccagcctacg accgattcgg tctcgggcaa
     1381 gatgccgggt ttgtccacca aagtgtcggc gaaatagcga atggtggaac ttcccgtcag
     1441 gcgtgccgca attgcgccca agacgggatg cagcgctaat tgcctcactt gtgagttctg
     1501 aagggcgacg acctcggcgg tacggacaat atttccgtcc cccggcttcc agtcggagaa
     1561 gccactcgta ggcggaaggt cccaatcccg ttcaccggag aaatgacgct gaatcccact
     1621 gaacgcatgg ttaagcagcg cctcaggcag cactcgcgtc gaacggtacc agccatgctg
     1681 gcgataaaag gccacgtctt cgtctgaagg caacggggcc tcatattgac tgagatccat
     1741 cgctgtgtcc taagaaggct ataagataaa gaagcggacg ggtgggacgt gtctctcgcc
     1801 gtcgctcatg aaaactgaag gcgcataatt gatcgggggg tgacgctctg taagagcggg
     1861 cccccacccc ccccccgcgc cccgtccccc cccccccacc gttggcccac aac
//
LOCUS       DAORQN010000273         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1598_length_1913_cov_5.139397, whole genome shotgun sequence.
ACCESSION   DAORQN010000273 DAORQN010000000
VERSION     DAORQN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_1913_cov_5.139397"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..456
                     /locus_tag="PK614_09700"
     CDS             61..456
                     /locus_tag="PK614_09700"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB82518.1"
                     /translation="MEKVSITQFLTEHIPDLIAIYQFGSQVHGTARPDSDIDLAVLAL
                     TRTPAERLFHLAQELAIQLGRTVDLLDLRSTTTVMRAQIISTGRCLESRDDQARAEFE
                     MYAYSDYARLNEERRELLKDIKERGLIYG"
     gene            449..862
                     /locus_tag="PK614_09705"
     CDS             449..862
                     /locus_tag="PK614_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRB82519.1"
                     /translation="MADDVVLNKAASIERRLARICEEYGGKQENLYENQTKQDAIVLN
                     LQRACETAIDLAMYVVRRKGLGLPQESRDAFALLEKAQLLPAELAGRLQRMVGFRNVA
                     VHEYTRLNPDIVQTIITTHLDDFRRFSSTIVKACA"
     gene            1510..>1913
                     /locus_tag="PK614_09710"
     CDS             1510..>1913
                     /locus_tag="PK614_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRB82520.1"
                     /translation="MSNLDDRMWATMPSRRSFLQRMGLGVGALGAGLFGGLALPESVL
                     AVCEPPGNPGTPKPWRKDCRMILPRRPASTLSAAEITQLKDAYKAMRALDSGAPNDPR
                     GFLRQANVHCWYCGAGTQVHFTWQFFAWHRAYL"
BASE COUNT          441 a          586 c          510 g          376 t
ORIGIN      
        1 ggtctacttc gacatttttc caggcgtcac gtatactccg atcaatcggc aggcataacg
       61 atggaaaagg tctcaatcac acaatttctc accgagcaca ttcccgacct gatcgccatc
      121 taccaattcg gctcacaggt ccatggcact gcgcggccgg acagcgacat cgatctcgcc
      181 gtgctggcac tcactcgaac acccgctgaa cggctctttc acttggcgca ggaactcgcc
      241 atacaactgg gccgtaccgt ggacctcctg gatctgagga gcacgacgac ggtcatgcgc
      301 gcgcagatca tcagcaccgg tcgatgcctc gaaagccgtg acgaccaggc ccgcgccgaa
      361 ttcgagatgt atgcctattc cgactacgcc aggctcaacg aagaacggcg agagctactg
      421 aaagacatca aagagcgcgg gctgatctat ggctgacgac gtggtgctga acaaggcggc
      481 aagtatcgaa cgtcgtctcg cccgcatctg cgaggagtat ggcggcaagc aggaaaatct
      541 gtacgagaat caaacgaagc aggatgccat cgtcctcaat cttcaacgcg catgcgaaac
      601 cgcgatcgat ctcgccatgt atgtcgtgag acgaaagggc ttgggccttc cacaagaaag
      661 cagggacgcg ttcgcactgt tggagaaggc tcaacttctc cctgccgagt tggccggccg
      721 actccagcgg atggtcggct tccgcaacgt ggccgtgcat gaatacactc gccttaaccc
      781 tgatattgtc cagacgatca tcaccacaca cctcgacgac tttcgccggt tctcctcaac
      841 catcgtcaaa gcttgtgcat agagggggga ccgtaccgca caagctgtca tcaccaacca
      901 cgccaattta actccccagt ctcagttcac gattcctctg tcagcgaatt ccacctactc
      961 agaggtggaa tcccggcaat cagcacattc agcgatccag aatggagaat cagcttaccc
     1021 ttctttcctt cgaggtcgaa tctgccataa gtcggacttg gctcacctga agtttcagaa
     1081 ccttccagtc cattgccaaa aagttgtagc gcagacggta gtccgccagc tgaggtcgca
     1141 acgataattt ctggatggac cagatccatt aaccccggat gccacagcta ggagagagaa
     1201 agcagcgctg cttttccttt gctgagatca ctagaagaat gagcgttcgc aaaaattgca
     1261 ttgggtgaaa aaaattccac cgcagctgta tctattcggg cgccaccgta tcccattgga
     1321 tacgaacacc tacgggattg acagggaatc cttcgacgcc aggccgagaa tccgcgcatc
     1381 tctcgcaacc gggggccgca tccgcttatg gcatggatat tgatcactcg ttgccacatc
     1441 caaccatggc gtcgcgctcg ggacggcgcg gagctttcct agccgaagcc gcatacaagg
     1501 aggtaggtca tgtcgaatct ggacgaccgc atgtgggcga ccatgccgtc gcggaggtca
     1561 tttcttcagc gaatgggatt gggtgtcgga gcgctaggag cgggcctgtt cggcggcctg
     1621 gcgttgccgg aatcggtgct ggccgtgtgc gaacctccag gcaatcccgg gacaccgaaa
     1681 ccatggcgca aggactgtcg aatgatcctc ccgcgccgcc ccgccagcac cctgagtgcg
     1741 gcggagatca cacaactcaa ggatgcctac aaggccatgc gcgcgctcga cagcggcgcc
     1801 cccaacgacc cgcgcggttt cctacggcag gccaacgtcc attgctggta ctgcggcgcc
     1861 ggcactcagg ttcatttcac ctggcaattc ttcgcctggc accgcgccta tct
//
LOCUS       DAORQN010000274         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1601_length_1909_cov_3.137001, whole genome shotgun sequence.
ACCESSION   DAORQN010000274 DAORQN010000000
VERSION     DAORQN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_1909_cov_3.137001"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PK614_09715"
     CDS             complement(<1..622)
                     /locus_tag="PK614_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB82521.1"
                     /translation="MMIHTCSPVRAPQPVIPLTYHIIGFLLLLLPVTLTHAPRAMANE
                     QGPHSESQVAQRAKAAWEQGTTDLALGILAQGIHENPRAFALHQLRGDMLATSRHTEA
                     ALESYETVLASIPSALDVRWAKWSVLVRSGQVEEAVTELQRIAAIDPQNPLIHLRLAR
                     ELRKLDRLEESLKSYQQAVELGPDMRNWRLAMARARFDILDYEGADR"
     gene            complement(931..>1909)
                     /locus_tag="PK614_09720"
     CDS             complement(931..>1909)
                     /locus_tag="PK614_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB82522.1"
                     /translation="ECDAILRALQGLTLHQARQVITQCIVEDGRLSAADVQTILKRKV
                     QAIKDGGLLEYYPLEDNRFELGGFVNLKSWLELAKVGFTAAAKALNLTPPRGIMLVGV
                     PGCGKSLAAKAIAREWQLPLLKLDAGRLFDKFVGESEKNFRKAIEMAESLSPIVLWID
                     EIEKAMVAGGGSGDADAGLSRRLFGAFLTWLQEKKQEVFVVATANNLASLPPELLRKG
                     RFDEIFFVDLPDDHERAAIWKIHLALRKQDSAGFDLEKIVSASDGFSGSEIEQAVVAA
                     LYRALHYKTPLTTDLLIQELSGTVPLSVTRQEDIDQLRTMAHGRFVNVR"
BASE COUNT          346 a          551 c          607 g          405 t
ORIGIN      
        1 cgcgatctgc gccttcgtaa tccaaaatat cgaagcgcgc gcgtgccatc gccaaccgcc
       61 agttgcgcat gtcgggtccc agctccacgg cctgctgata ggacttcaag gactcctcca
      121 gccggtcgag tttccggagc tcacgggcca gtcgcaaatg gatcagcggg ttctggggat
      181 cgatggcggc aatgcgttgc aattcagtga ccgcttcttc cacctgcccc gatcgcacca
      241 gcacgctcca cttggcccat cgtacgtcca gcgcgctggg aatactcgcc aggacggttt
      301 cgtaggactc aagggccgct tcggtgtggc gtgaggtggc gagcatgtcg ccgcggagct
      361 ggtgcagcgc gaatgctcgc gggttctcat gaatgccttg ggcaagaatg ccgagggcca
      421 ggtctgtcgt gccctgctcc caggcggctt tcgcgcgttg ggcgacctgg ctttccgagt
      481 gggggccctg ctcgttcgcc atggccctgg gggcgtgtgt cagcgtgacg ggcaggagta
      541 ggaggagaaa tccaatgatg tgataggtca acgggatgac cggctgcggt gcgcgaacgg
      601 gcgaacatgt atggatcatc atgaggtgag tctctggttg aggtgaacaa gtcccttcca
      661 ttgtcaggcg ggagatcggg tagatagcgg tatccccgag catgcctgtc tgtattcgat
      721 tagaggcacc gtgaggtaga aggattcgtc gccctcagcg gttccaggca caacgaatta
      781 tcttactgta cgatggcccg gctctgcgag gtgccccgga cagatccggc tgagggtggg
      841 gcatgagacg catcctgtct ggaagggccc ggtcagccgg tgaatgtggt caccgaaggg
      901 gttgtctcga tggagcggaa tcgaatccgc ttaccgcacg ttgacgaagc ggccgtgagc
      961 catggttcga agctgatcga tgtcttcctg gcgagtgacc gagagcggca cggtgccgga
     1021 taattcttga atcaagagat cggtcgtcag cggggttttg taatgtaagg cccggtagag
     1081 tgcggcgacc accgcctgtt cgatttccga gccgctgaaa ccatcactgg cgctgacgat
     1141 tttctcgagg tcgaatccag cgctgtcctg tttgcgaagc gccaggtgga tcttccagat
     1201 ggcggcccgt tcgtggtcgt ccggcaagtc cacgaagaaa atttcgtcga accgtccttt
     1261 gcgcagcagt tccggcggga gggaggcgag gttgtttgcc gtggcaacga cgaacacctc
     1321 ctgcttcttt tcctgcagcc aggtgagaaa ggcgccgaag agccggcggc tcaatccggc
     1381 atcggcatct ccgctcccgc cgccggccac catcgccttc tctatttcat cgatccagag
     1441 cacgatcggc gagagggatt cggccatctc gatcgccttc cggaaattct tctccgattc
     1501 gccgacgaat ttgtcgaaga gccgcccggc atcgagtttg agcagcggga gttgccactc
     1561 gcgtgcaatg gcttttgccg cgagcgactt gccgcagccc gggacaccca ccagcatgat
     1621 gccgcgcggc ggcgtcaggt tgagcgcctt ggctgctgcc gtgaatccca ccttcgccag
     1681 ttccagccag gatttcagat tcacgaaccc gcccagttca aaccggttgt cttcgagggg
     1741 ataatattcc agcaaccctc cgtccttgat cgcctggacc ttgcgtttca ggatggtttg
     1801 tacgtcggca gcagacagtc tgccgtcttc gacgatgcat tgcgtgatga cctgccgggc
     1861 ttgatgcagc gtcaatcctt gcaaggcgcg gaggatggcg tcgcattcc
//
LOCUS       DAORQN010000275         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1621_length_1878_cov_8.121777, whole genome shotgun sequence.
ACCESSION   DAORQN010000275 DAORQN010000000
VERSION     DAORQN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_1878_cov_8.121777"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK614_09725"
     CDS             complement(<1..823)
                     /locus_tag="PK614_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB82523.1"
                     /translation="MAYTSLKKAAEQQLAEIRAAGLYKTERQILSPQSTEIHVAQGDV
                     VNLCANNYLGLANHPEVRQAAADGLREFGYGMASVRFICGTQRLHKTLEQAISTFLGT
                     ADTILYSSCFDANGGLFETLLDERDTIISDALNHASLIDGIRLCKAARLRYAHADMAE
                     LETRLGESASSRVRMIVTDGVFSMDGDLAKLDRIVELADRYDAAVVVDDSHATGVLGK
                     GGRGTPDHFGVAGRIDLVTSTLGKALGGATGGFTTGRQELIELLRQRSRPYLFSNS"
     gene            complement(907..>1878)
                     /gene="tdh"
                     /locus_tag="PK614_09730"
     CDS             complement(907..>1878)
                     /gene="tdh"
                     /locus_tag="PK614_09730"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRB82524.1"
                     /translation="TPGPSEAVIRVKATSLCGTDAHIYNWDAWAHSRIHPPRTIGHEM
                     CGEVVAVGADVTLVRVGDYVAAESHLTCGACFQCRTGQAHVCKNYRILGVDLDGSFAD
                     YVVLPETVLWKTSPDIPPELACLQEPLGNAVDAALVEDLTGHTVLITGCGPTGLFAAA
                     VARTAGAATIIATDVSDYRLSLAKQVGVDHVFNAKTDGPEAIAAAILDITRGEGVDAS
                     LEMSGHPTALHHAFRSVKNGGRVTLFGIPTGPVTCDLANEVIFKGIRVHGITGRRLFS
                     TWYRLAGLFKAGLNIRPVLTHTFPLKEFAQGFELIKSGQCGKVVLFP"
BASE COUNT          350 a          529 c          603 g          396 t
ORIGIN      
        1 agctattcga aaagagatag ggccgtgagc gttgtcgtaa cagctcgatc agttcttgcc
       61 tgccggtggt aaatcctccg gttgccccgc ccagcgcctt gccaagggtg ctcgtgacga
      121 ggtcgatccg cccggcgact ccgaaatggt ccggggtgcc gcgtccgcct tttcccaaca
      181 ctccggtggc atgactgtcg tcgacgacca cggcagcatc gtatcgatcg gccagctcca
      241 caatgcggtc cagcttcgcc agatcgccgt ccattgaaaa caccccgtcg gtcacgatca
      301 tgcgcacgcg gctcgatgcc gattccccca gtcgtgtttc caactcggcc atgtcggcat
      361 gggcatagcg cagacgggcg gctttgcaga gccgaatgcc gtcgatcagg ctcgcgtggt
      421 tcaaggcatc gctgatgatg gtatcccgct cgtcgagcag ggtttcgaac agtccgccgt
      481 tggcgtcgaa gcaggagcta tagaggatgg tgtccgcagt gcctaagaag gtgctgatcg
      541 cttgttcgag tgttttgtgc agtcgttgtg ttccgcaaat gaatcgcacg gaagccatgc
      601 catagccgaa ttccctgagt ccgtcggcgg ccgcctgtct cacctccgga tgattcgcca
      661 gcccgagata gttgtttgcg cagagattga cgacgtcccc ctgtgccaca tgaatctcgg
      721 tgctctgcgg gctcaggatc tgccgttcgg tcttgtagag accggctgca cggatctcgg
      781 ccagttgttg ttcggcggct tttttgagcg aggtgtaggc catcttcctc gtgaaaggtg
      841 aaacgtgaaa cggttgaggg aagttcagat atccgatccg tttcgtggaa ctctggcccc
      901 tgtcgtttat ggaaacagga ctactttgcc gcattggccg gatttgatca attcaaatcc
      961 ttgggcaaat tctttgaggg ggaaggtatg ggtgaggacc ggacggatat tgaggccggc
     1021 tttgaagaga ccggccaggc gataccaggt gctgaatagg cgacgaccgg taataccatg
     1081 gacacggatg cctttgaaga tgacctcgtt ggcgaggtcg caggtgacgg ggccggtggg
     1141 aatgccgaac agcgtgacgc ggccgccgtt tttcaccgag cggaaggcgt ggtgcaaggc
     1201 cgtggggtgt ccggacattt ctaacgacgc atcgacgcct tctcccctcg tgatgtcgag
     1261 aatcgctgcg gcaatggcct ctgggccgtc ggtcttggcg ttgaatacgt gatcgacgcc
     1321 cacttgtttg gccaggctca ggcggtaatc actgacgtcg gtggcgataa tggtcgcggc
     1381 tcctgcggtg cgggcgacgg ccgccgcgaa caggccggtc gggccgcagc cggtgatcag
     1441 aacggtgtgg ccggtcaagt cttccacgag ggccgcatcg acggcattgc ccaaaggttc
     1501 ttgtaggcaa gccagctccg gcggaatgtc cggagacgtc ttccaaagca cggtttcagg
     1561 gagcacgacg tagtcggcaa acgagccatc cagatcgacg ccgagaatcc ggtagttctt
     1621 gcacacgtgg gcctggcccg tgcggcattg aaagcaggcg ccgcaggtca ggtgcgattc
     1681 ggctgcgaca tagtcgccga cgcgcacgag cgtgacatcc gccccgacgg ccactacctc
     1741 gccgcacatt tcatgtccga ttgtcctggg aggatgaatg cggctgtgag cccaggcatc
     1801 ccagttatag atgtgggcat cggttccaca cagcgacgtg gctttgaccc ggatgacggc
     1861 ctcagatggg ccgggtgt
//
LOCUS       DAORQN010000276         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1624_length_1876_cov_3.895662, whole genome shotgun sequence.
ACCESSION   DAORQN010000276 DAORQN010000000
VERSION     DAORQN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_1876_cov_3.895662"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK614_09735"
     CDS             complement(<1..472)
                     /locus_tag="PK614_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRB82525.1"
                     /translation="MLPWRSHSLWFNLPFLVCAWLSLLSSAGLAQSSGAHEAGQGREP
                     GLIGPVNHRAEIQEPSTLPKDLSGSLDWENGRGRSYLIPAAEVLTYLFLLNQYDRHFT
                     EPKDVYRTTGTTIREHLTDSKWVFDNDQFSVNQFLHPYGGSVYFGLARSAGISFW"
     assembly_gap    624..703
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<704..>1013)
                     /locus_tag="PK614_09740"
     CDS             complement(<704..>1013)
                     /locus_tag="PK614_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82526.1"
                     /translation="RLSPRHRHLAQAQQTIAAQNGALVPAPAHAFNPTLRRSLLVPSP
                     SMQSRHAPPLLNQQGLPAKVGDIVTIPTPRSIPPVMHQSAPQPQPYQLHPSHFWRPSP
                     S"
     assembly_gap    1014..1031
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1032..>1876)
                     /locus_tag="PK614_09745"
     CDS             complement(<1032..>1876)
                     /locus_tag="PK614_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRB82527.1"
                     /translation="ITMSLSRSRLTLSVLFLLGVSTQVITAPDSRASIYTYTDEAGVQ
                     TFTNQLESVPEQYRNRLTSQELDTPPAVSSAPPRTVAESPTRSADSRIVTANGEYRLG
                     DHDTRTDGARLAVEAAKRDALEQVATYLERVTEVRDMNLTRDDIRSYTAGIVKVLEET
                     ITTRVENESIVIRAELTAEIDPHEVAQAIAALRENDSAMLELTVLRAETDRLQEQLDA
                     TNRALAAAPSAEEVQQLSRQRDDMLNDLQANALVSQAWTSWAYPTLGFYSYPWIAGPG
                     INGLL"
BASE COUNT          325 a          482 c          603 g          368 t
ORIGIN      
        1 cccagaagct gatccctgcg gatcgggcca gaccgaaata cacgctgccg ccatagggat
       61 gcaggaactg gttgactgag aactgatcgt tgtcgaacac ccatttcgaa tccgtcaggt
      121 gttcacgaat ggtcgtgccg gtggtccggt acacgtcctt tggctcagtg aaatgccgat
      181 cgtactggtt cagcaggaac aaataggtga gtacttcagc ggctggaata aggtaactgc
      241 gccctcgccc gttctcccaa tccaacgatc cggacaggtc tttgggcagg gttgatggtt
      301 cttggatttc agcacgatgg ttcacgggcc cgatcaggcc aggctcgcgc ccttgcccgg
      361 cttcatgtgc gcccgaactt tgtgccaggc ccgcactcga cagaagcgac agccaggcac
      421 agacaaggaa gggcagattg aaccacaggc tgtgcgaacg ccaaggaagc atgctgcgtc
      481 atatgccttc cctcatcagt tgaacgcagg gggccgctgc ctgggtactc ggaggaaccc
      541 tagtccatgc tcaaccgacc tgtctcgggt gggccgtccg gccttcgcga tcagcggcct
      601 cgcccgccgc tgttgccgcg agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgaagg gctggggcgc
      721 cagaaatggg acgggtgcaa ctggtagggc tgcggctgcg gtgcagactg gtgcatcacc
      781 ggtggaatgg agcgcggtgt gggaatggtc acgatgtctc ctactttggc cggtaggccc
      841 tgctggttga gcaacggtgg cgcgtgacga ctctgcatcg atggtgatgg cacgagcaag
      901 gagcggcgca gcgtgggatt gaaggcgtga gccggggccg gtacgagcgc gccgttttgc
      961 gccgcaatgg tctgttgtgc ctgcgcgagg tggcgatgcc ggggagagag gcgnnnnnnn
     1021 nnnnnnnnnn ntagtaagcc gttgatccca ggtcctgcga tccagggata ggaatagaag
     1081 ccgagcgttg ggtatgccca gctggtccag gcctgtgaga caagggcatt ggcctgcagg
     1141 tcgttcagca tgtcgtcacg ttggcggctg agttgctgga cttcctccgc cgatggggcg
     1201 gcggcgaggg cgcggttggt ggcatcgagc tgttcctgta gtcggtcggt ttcggcacgg
     1261 aggaccgtga gctccaacat ggcgctgtcg ttttcccgca aggccgcaat ggcctgtgcg
     1321 acttcatgcg ggtcgatctc tgccgtcaac tcagcgcgga tcacgatact ctcattctcg
     1381 accctggtcg tgatggtttc ctccaacacc ttgacgatgc ctgccgtgta actccggatg
     1441 tcatcacgtg tgaggttcat gtcgcgcact tccgtaacac gttccagata cgtggccacc
     1501 tgttcgagcg cgtctctctt ggcggcttcg accgccagac gcgcgccatc cgtgcgggtg
     1561 tcgtgatcgc ccaagcgata ttcgccgttg gctgtgacga tgcgggaatc ggcggagcgc
     1621 gtcggtgatt cggcaacggt gcgaggcgga gcagaggaaa cggctggagg cgtgtcgagc
     1681 tcctgtgatg tcagcctgtt tcgatattgt tcggggacgg actcgagttg gttcgtaaag
     1741 gtttgaacac cggcctcgtc ggtgtaggta taaatcgagg cccggctgtc tggtgcggta
     1801 atgacttggg tggagacccc gagaagaaag agaaccgaca gtgtcagcct actgcgggag
     1861 agagacatgg tgatcc
//
LOCUS       DAORQN010000277         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1625_length_1875_cov_86.391209, whole genome shotgun sequence.
ACCESSION   DAORQN010000277 DAORQN010000000
VERSION     DAORQN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1625_length_1875_cov_86.391209"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1620
                     /locus_tag="PK614_09750"
     rRNA            83..1620
                     /locus_tag="PK614_09750"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1682..1758
                     /locus_tag="PK614_09755"
     tRNA            1682..1758
                     /locus_tag="PK614_09755"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Ile,seq:gat)
BASE COUNT          499 a          396 c          553 g          427 t
ORIGIN      
        1 aattactcga agtttatttg agaaatattt gtcagaaaat tgatgagcca agattggcgc
       61 cctttaaggc gctactgatt ttaaactgaa gagtttgatc atggctcaga ttgaacgctg
      121 gcggcaggct taacacatgc aagtcgagcg gagatgaggt gcttgcacct tatcttagcg
      181 gcggacgggt gagtaatgct taggaatctg cctattagtg ggggacaaca tttcgaaagg
      241 aatgctaata ccgcatacgc cctacggggg aaagcagggg atcttcggac cttgcgctaa
      301 tagatgagcc taagtcggat tagctagttg gtggggtaaa ggcctaccaa ggcgacgatc
      361 tgtagcgggt ctgagaggat gatccgccac actgggactg agacacggcc cagactccta
      421 cgggaggcag cagtggggaa tattggacaa tgggcgaaag cctgatccag ccatgccgcg
      481 tgtgtgaaga aggccttttg gttgtaaagc actttaagcg aggaggagga gctctagatt
      541 aatactctag atgcttggac gttactcgca gaataagcac cggctaactc tgtgccagca
      601 gccgcggtaa tacagagggt gcgagcgtta atcggattta ctgggcgtaa agcgtgcgta
      661 ggcggcttct taagtcggat gtgaaatccc tgagcttaac ttaggaattg cattcgatac
      721 tgggaagcta gagtatggga gaggatggta gaattccagg tgtagcggtg aaatgcgtag
      781 agatctggag gaataccgat ggcgaaggca gccatctggc ctaatactga cgctgaggta
      841 cgaaagcatg gggagcaaac aggattagat accctggtag tccatgccgt aaacgatgtc
      901 tactagccgt tggggccttt gaggctttag tggcgcagct aacgcgataa gtagaccgcc
      961 tggggagtac ggtcgcaaga ctaaaactca aatgaattga cgggggcccg cacaagcggt
     1021 ggagcatgtg gtttaattcg atgcaacgcg aagaacctta cctggtcttg acatagtaag
     1081 aactttccag agatggattg gtgccttcgg gagcttacat acaggtgctg catggctgtc
     1141 gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttttcctta
     1201 tttgccagcg ggttaagccg ggaactttaa ggatactgcc agtgacaaac tggaggaagg
     1261 cggggacgac gtcaagtcat catggccctt acgaccaggg ctacacacgt gctacaatgg
     1321 tcggtacaaa gggttgctac ctagcgatag gatgctaatc tcaaaaagcc gatcgtagtc
     1381 cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc gcggatcaga
     1441 atgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggaattt
     1501 gttgcaccag aagtaggtag tctaaccgca aggaggacgc ttaccacggt gtggccgatg
     1561 actggggtga agtcgtaaca aggtagccgt aggggaacct gcggctggat cacctcctta
     1621 acgaaagatt gacgatcggt aagaatccac aacaagttgt tcttcatgaa gatgtatctg
     1681 agggtctgta gctcagttgg ttagagcaca cgcttgataa gcgtggggtc acaagttcaa
     1741 gtcttgtcag acccaccaaa tctgactaac aagcattatt aaatgctgaa tacagaaaaa
     1801 cagagacatt gacttattga taagctgggg acttagctta gttggtagag cgcctgcttt
     1861 gcacgcagga ggtca
//
LOCUS       DAORQN010000278         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1629_length_1869_cov_7.751378, whole genome shotgun sequence.
ACCESSION   DAORQN010000278 DAORQN010000000
VERSION     DAORQN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1629_length_1869_cov_7.751378"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1727
                     /locus_tag="PK614_09760"
     CDS             162..1727
                     /locus_tag="PK614_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB82528.1"
                     /translation="MDSVAKETKHIASLAPDERASAYIERSKARLALRDYQSAIADLK
                     EAVRLEPDRAEVYDELRKFYEATGQGFDERVARGWFEYYRQQYDVAVRIFGLAITLKP
                     KQAEPWWGRAKATLKKGRNANDALDDLNQALQLDRTLMNAYLERGKIHRAAGRFDLAV
                     ADLDTFLKSKPEHVDGLVERGTALFSLGRRTQALADFTRALTVNPLHEQALVRRAYLL
                     FEEGNHEGAWQDADTVRRMGAGNPEALLLLGRLSYMKENFVEAVSLYDRAVRTAPGFS
                     QTAINVYDRIVERAPRLGATYHARGVAHYQDNRLEAAIKDLSLALQLMGEQPQALYDR
                     GVAYLRSRRFQEAAADFERALKHREQFDTTILSDPVQFGVGSGHDRLLGHQFGAAHPS
                     VFINLAWTYYLLGRYEEARVAIGRGLQRYDESDTNYLAGIISARLGDMTNAVHSVERA
                     ARSEPRNSLYADTLTKFTKERNRAAIESWLGEMVTLGAIGLIGAGYNDYLCDLDEDCS
                     WRRDKARERKQGR"
BASE COUNT          424 a          486 c          543 g          416 t
ORIGIN      
        1 tttcatattc cactatcgtg gcgcaaaaaa gaatgtagat aacgatgcgt ctctgttgga
       61 ccgctagcgc aattctcctt ggaggcactt atctcgctgg ctgcgccagc gtgccgaaga
      121 attccgctcg cccgccacta tccttcaagc aagaacaagc gatggactcc gtcgccaagg
      181 aaactaagca cattgcatcg ctcgctccgg atgaacgtgc cagcgcgtat attgaacgga
      241 gcaaagctcg tcttgcgtta cgggactatc aatccgccat tgcggatctc aaggaagccg
      301 ttcgcttgga gcccgatcgc gcggaggtgt atgacgagct tcgcaaattt tatgaggcga
      361 ccggacaggg cttcgacgaa cgtgtggccc gtggctggtt tgagtattat cgtcagcagt
      421 acgacgtggc agtgcggatc tttggcttgg cgattactct gaaacccaaa caggccgagc
      481 catggtgggg acgggctaaa gcgacactga agaaagggcg taacgccaac gacgctttgg
      541 atgatttgaa tcaggcgctg caactcgatc ggacattaat gaatgcctac ctggagcggg
      601 gaaaaatcca tcgtgccgcc ggtcgcttcg atcttgcggt ggccgatctc gatacctttc
      661 tcaagtcgaa acccgagcat gtggacgggc tagtcgaacg gggaacagcg ttattttcgc
      721 ttggacgcag aacacaggcg ctggccgact tcacgcgcgc gctgaccgtc aatcccttgc
      781 acgaacaagc cttggtaaga cgtgcgtacc tccttttcga agaggggaac cacgaaggcg
      841 cttggcagga tgcggatacc gttcgccgaa tgggcgccgg caatcccgaa gcccttctcc
      901 ttctaggtag gctgtcttat atgaaggaga atttcgtcga agccgtttca ctctacgacc
      961 gggctgtacg cactgcgccg gggttctctc aaacggccat caatgtctat gaccgaatcg
     1021 tcgagcgggc gccgcgcctc ggtgcgacct accatgcgag gggcgtggcc cattaccaag
     1081 acaaccgcct ggaggcggcc attaaggacc tgtcgctcgc tcttcaattg atgggggagc
     1141 agccacaagc cttgtacgac cgaggggtgg cgtatctgcg aagtagaaga tttcaagaag
     1201 ccgccgctga ttttgaacgc gcgctgaagc atcgtgaaca gttcgacacg acgatcctgt
     1261 ccgatcctgt gcagtttgga gtgggttctg gccatgatcg tcttctgggg catcagttcg
     1321 gagcggctca cccctctgtc ttcatcaatc ttgcctggac atattatctt ctgggccgct
     1381 atgaagaagc gagagttgcg atcgggcgtg gtcttcagcg atatgatgag tccgacacca
     1441 attatctcgc gggaataatt tctgcgcgac ttggcgacat gaccaatgcg gtgcattcgg
     1501 tggaacgtgc agcgcggagc gaacctcgca attcgttgta cgcagacacg ttaacgaagt
     1561 ttacgaaaga acgaaatcgt gccgcgattg agagttggct gggagagatg gtcacattag
     1621 gggcgattgg cctcatcggt gctggttaca acgattacct ctgtgacctg gatgaggatt
     1681 gtagctggag gcgcgataag gcgagagaaa gaaagcaagg aagatgatcg gaggatgagt
     1741 tgaaacacgc cttcgtgacc gtgagtcttc gcccagtcct taccataagt gtctggcttg
     1801 catccttcct gtttccatcc gcatgcgttt cgacgaaaag gcaactaact gtcgccaatt
     1861 tgtacgtaa
//
LOCUS       DAORQN010000279         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1633_length_1861_cov_6.830011, whole genome shotgun sequence.
ACCESSION   DAORQN010000279 DAORQN010000000
VERSION     DAORQN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1633_length_1861_cov_6.830011"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..311)
                     /locus_tag="PK614_09765"
     CDS             complement(114..311)
                     /locus_tag="PK614_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82529.1"
                     /translation="MQEQSDVFVTVDRNLSFQQHLPQFNIAVIVLQAPSNRLKDLRPL
                     MPQFNRTVLVASKGQVTLVTL"
     gene            945..1214
                     /locus_tag="PK614_09770"
     CDS             945..1214
                     /locus_tag="PK614_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82530.1"
                     /translation="MNAEQMEEIKRHFGVVAESLRSDIQQIAEGHATIRHELQDLRGE
                     FRDEFKEMRALMRLSFSQIDQRIRTLESDISTLKARMDRIETSRA"
     gene            complement(1485..1646)
                     /locus_tag="PK614_09775"
     CDS             complement(1485..1646)
                     /locus_tag="PK614_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82531.1"
                     /translation="MKQTQTLSALFSVPGFRARSRLHGIFGDPHARLVVFVRRKKLPC
                     APAAEPATT"
BASE COUNT          441 a          516 c          521 g          383 t
ORIGIN      
        1 gaagaaatga ggtagaatgc gagaagaagt gccatcacta accctccctt ctacttaatt
       61 tatgtgctag gaggggcttc tgtcaatcgg acttgtcact ggctatcggc aacctacaga
      121 gtcaccaacg tcacttgccc ctttgaagcc acgagcaccg ttcgattgaa ttgaggcatc
      181 aacggacgta ggtctttcaa gcgattgcta ggcgcctgca acacaataac ggcaatgttg
      241 aattggggaa ggtgttgttg aaaggagagg ttgcggtcaa ccgtcacaaa cacatcggac
      301 tgttcctgca cgcgccggag cagctctccg ttctggatac cggctcaacc ggtctgcggc
      361 actgtaacga cctcatggcc atcgatctct tgggccaggc gacgggtcga tacactcgtc
      421 gagaagaatt ttcacgaagc gacaggaagc attcggatcg ttgcttcttc gaggaaagcg
      481 atcacttgct cacgcgtgac agtggggaac ccgtcgagga actcgtcgat cgtctgcccc
      541 cctctaaata ctcgatgagc gtctgcacgg gcacgcgagt accgcgaaaa acgggcgtcc
      601 cgcctaaaac gtcaggagtc gaggtaatga ggggttgctt ggcagtcgtc atcacacatc
      661 ctaatcagta cgtggaattt taagccgggc ggcaggagaa aacaatgaga agaccgccgt
      721 gcagtccgca tcacggatgc cggtaggccg aatgacgagt tgccaccggg cgccttgaat
      781 agttacttca aacaggccga gtggaatacg ttgagatctc cgcgtagata ttcctcgcac
      841 gatctcattc agattgtgaa catgagtacg caccacacag gacgccgccg ctagggcggg
      901 acgaagcgtt gcatttttga gcagacacgt ctataaaagc gcccatgaac gccgaacaga
      961 tggaagagat caagcgccat tttggggttg tggccgaatc actccgaagc gatatccaac
     1021 agatcgccga gggtcacgcc acaattcggc atgaactcca ggacctgcgc ggtgagttca
     1081 gggacgaatt taaggaaatg cgggccttga tgagactgtc gttttcccag atcgaccagc
     1141 gaatccgaac gctcgaatca gatatttcga cactcaaagc ccgcatggat cgcatcgaga
     1201 ccagcagggc ataactttca tccatacaac ctatgccgtc gcgcgcgcga acacggatct
     1261 gactgtatta catcgggcga tagctcgtcg gtggttaggc ttatcgaatc tctgccgcct
     1321 gccgttcggc cgtcacacgc tccggcagaa ttccggtcgc gtcacgctca gaggagacgc
     1381 ctccacatct gagacgccca aggcgatatg aaatcgcctt gggctctcct tttcagacag
     1441 agcgatgaca aacccctaat gtttaattgc aagacctggc cccattaggt ggtggcgggt
     1501 tccgcagccg gagcacacgg cagttttttt cgccggacga agacgacgag tcgggcgtgg
     1561 ggatcgccaa agatcccgtg cagccgactg cgggcgcgga accctggaac ggagaacaag
     1621 gcggagagcg tctgggtttg tttcatgggt cctcccggac aactgccgcc aggagtctgc
     1681 cacagatctc ggacacgatt cgaggtctcg ccacccgggc ccaaccccag gctggtcgcg
     1741 gagttgggaa gcctcacgcg ctctcattta tccactccaa tctgcgtaga gccattagtt
     1801 tttctcgata cgacgtcccc ccgtcttgag tagcagctac gtttggagtc cggatgcgac
     1861 t
//
LOCUS       DAORQN010000280         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1639_length_1851_cov_3.114699, whole genome shotgun sequence.
ACCESSION   DAORQN010000280 DAORQN010000000
VERSION     DAORQN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1639_length_1851_cov_3.114699"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    222..296
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            611..1270
                     /locus_tag="PK614_09780"
     CDS             611..1270
                     /locus_tag="PK614_09780"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82532.1"
                     /translation="MTISRARLILLACASGLLYPLSFPAFDLSIVAWFALVPLHIAVE
                     YLSPGRAFRIGWLSGTLAFTGSMFWVITAMNVYGKVPFPIATLVMLLLTVYLGLYMAV
                     YAGGLSWIRTAFPTLGFLPAPFLWVTLELIRTYFLSGLPWALFGYSQYQWLPIIQLAD
                     HFGVYGVSFLLVLVNAALAETIIWGFKAYRGFQIRSFPWPSSVAATARFGVPRDYGTP
                     H"
     gene            1328..>1851
                     /locus_tag="PK614_09785"
     CDS             1328..>1851
                     /locus_tag="PK614_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRB82533.1"
                     /translation="MEQAQKWDVAFRQETMARYDRLTAGLGDNLDLSVWPEAATPFVF
                     EMEAQYRAVVNDMARRAGAPLLFGSPALRRHPDGRPFLLNSAYLLATDGQILGRYDKQ
                     HLVPFGEYIPFHNSFLFFLDKLVEGIGDFEAGPGSTLLMFKPREKNPPAVTAATLTDF
                     HVKFGVVICYEVIFP"
BASE COUNT          318 a          589 c          463 g          406 t
ORIGIN      
        1 ccgggcatgt gctctcccgc atcattcaac atttgaatag cgtgattcac ctcccaggaa
       61 tagacatgca gtacaatgtg gcaagagatc cctatgcgta tcttcagagg gagtgtccta
      121 tgccaaccac cagcctgctg atgctactta ccgcatggac gctattaggc ccggtttcca
      181 tcgccggctc ccagccatcg atccttcaac agcgggacgg gnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgg
      301 ggtccggcct tccgtcacac acgttcagtt ctccccatgg cgcagtgccc ggatcagtca
      361 caccattcgg aacaccgaca ccgccgaatc tcgtcacacc ggcgccgtta ctgccgctcc
      421 aaccaagagg tatggcgact ccatacccac agccccccgc atcatccgat agtccaggcg
      481 gatacagcag gttcggcggg cgatcggggc gctaaccggt cgaacgacca gtctgggatg
      541 atcctccccc aagcccatcc gccaaagagc acggttgaat ctcttcagtt ctttcgttaa
      601 gttacaggca atgaccatct cccgcgcacg cctcattctg cttgcctgcg cgagcggtct
      661 tctctatccc ctctccttcc ccgcgttcga cctcagcata gtggcctggt tcgcgctggt
      721 cccgctgcat atcgcggtcg aatacctgtc gccgggtcgc gcgttccgta tcggctggct
      781 ttccggcacg ctggccttca cgggctcgat gttttgggtc attaccgcca tgaacgtcta
      841 cggtaaggtc ccgttcccga ttgccaccct ggtgatgttg ttgctcacgg tctacctcgg
      901 actctacatg gccgtctacg caggcggact ttcgtggatc cggacagcgt tcccgacgct
      961 gggcttcctg cccgcgccgt ttctctgggt gacattggaa ctgatccgta cctactttct
     1021 ctccggctta ccctgggcac tattcggata ttcccaatac caatggctcc cgatcattca
     1081 gctggccgac catttcgggg tgtacggcgt ctctttcctc ctcgtgttgg tcaacgctgc
     1141 gctggctgaa acaattatct ggggcttcaa ggcctatcgc ggatttcaga ttcgttcgtt
     1201 tccgtggccc tcgtccgtgg cggctaccgc ccgattcggc gtcccccggg attatggcac
     1261 cccccactag tcgtccgcca cgcatgtcca gacccgcacc ttggcggtgg gagtcgtaca
     1321 accgaatgtg gaacaggcac agaaatggga tgtggcattt cgccaggaga cgatggcgcg
     1381 ttacgatcga ctcactgcgg gactgggcga caacctggat ctcagcgtct ggccggaagc
     1441 cgccacaccc tttgtatttg aaatggaagc gcagtaccgc gcagttgtga acgacatggc
     1501 tcgccgcgcc ggtgccccgc tgctattcgg cagcccggct ttgcgacgtc atccggacgg
     1561 tcgccccttc ttgctcaaca gcgcgtacct gctcgccacc gacggccaga tcctcggtcg
     1621 ctacgacaag caacatctgg ttcccttcgg cgaatacatt ccgtttcaca attcattctt
     1681 gttcttcctg gacaaactcg tcgaaggcat cggcgacttc gaagccggac cgggatcgac
     1741 gttattgatg ttcaagcccc gcgagaagaa cccgcccgcg gtgaccgcag ccaccctcac
     1801 cgatttccac gtgaagttcg gcgtcgtcat ttgttatgaa gtgatcttcc c
//
LOCUS       DAORQN010000281         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1642_length_1847_cov_7.180804, whole genome shotgun sequence.
ACCESSION   DAORQN010000281 DAORQN010000000
VERSION     DAORQN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_1847_cov_7.180804"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /gene="fsa"
                     /locus_tag="PK614_09790"
     CDS             <1..626
                     /gene="fsa"
                     /locus_tag="PK614_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRB82534.1"
                     /translation="NVKEIQEGANLGLIDGVTTNPSLVAKEGRSFKEMLLEICKMVDG
                     PISAEVVGVESEAMIKEGRDLAKVHKNIVVKVPLIPEGLRATKKLAAEGIRVNVTLCF
                     SPTQALLAAKAGAWCVSPFIGRLDDVSSDGMALIRQIVTIYKNYDYKTQVLVASVRHP
                     QHVVEAALAGGHICTMPYAVFQQLVKHPLTDSGLKKFLADWDAMGKK"
     gene            complement(640..1338)
                     /locus_tag="PK614_09795"
     CDS             complement(640..1338)
                     /locus_tag="PK614_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRB82535.1"
                     /translation="MSALTMLNNEIVACTCCPRLVAYREAVARDKRRQFREWSYWGKP
                     VPGFGDSNARLYILGLAPAAHGGNRTGRVFTGDRSGDWLYEALHRFGFANQATSIHAG
                     DGLTLTNCYIGATVRCAPPANKPTPDEFTACRPFVLRELRLLKRMRVIVTLGKIAFDH
                     YLKASRELGLGTPGLRPQFGHGVVYDLPWGVTLIGSYHPSQQNTFTGKLTRPMFHRIF
                     LKAQQKLAVAPLRD"
     gene            1433..>1847
                     /locus_tag="PK614_09800"
     CDS             1433..>1847
                     /locus_tag="PK614_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82536.1"
                     /translation="MWNKERGVQAFLACVTMLFGLSSAMPVLSADSSSAALAGAKAEE
                     GRNAQESDQVPVAPPGESELVPELEDRLVILPEIKREGERFFLSSFKLPDKLTFAGQA
                     IPLDNWQVRERIEYEFYQFLEDQGESIILAKRTGRC"
BASE COUNT          398 a          473 c          581 g          395 t
ORIGIN      
        1 ccaatgtgaa agaaatccaa gagggtgcca acctgggcct catcgacggt gtgacaacta
       61 atccatctct tgttgccaag gaagggcgca gtttcaagga gatgcttctc gaaatctgta
      121 agatggtcga tggcccgatc agcgcagagg tggttggtgt cgaatccgag gccatgatca
      181 aagaagggcg agatctggcc aaggttcaca agaatatcgt ggtgaaggtt ccccttatcc
      241 cggaaggtct tcgggccaca aagaagctcg cggcagaggg gattcgtgtg aatgtcacct
      301 tgtgcttctc cccgacgcag gctttgctgg ccgcgaaggc gggcgcgtgg tgtgtatcgc
      361 cattcattgg tcgtctcgac gacgtgagtt cggatggaat ggcattgatt cgccagatcg
      421 tcacgattta taagaactac gattataaaa cgcaggtcct cgtcgcgagt gtccgacatc
      481 cgcaacacgt cgtcgaagca gcgttggccg gggggcacat ttgcacgatg ccttacgccg
      541 tgtttcagca gcttgtgaag catccgctga ctgacagtgg gctgaagaag tttctggccg
      601 attgggatgc aatgggaaag aaatagactt tcgaagcggc taatcgcgca agggtgccac
      661 ggcgagtttc tgctgggcct tgagaaaaat gcggtggaac attggtcgag tcaacttgcc
      721 ggtgaacgtg ttttgctggc tgggatggta tgagccgatc aaggtcacgc cccaaggtaa
      781 gtcgtagacg accccgtgtc caaattgagg gcgtagcccg ggagtgccta gccccaattc
      841 acggctcgcc ttgagatagt gatcgaatgc aatcttgccc aaggtgacga tcacacgcat
      901 tcgcttcaat agccgtagtt cgcgcaggac aaatggccgg caggccgtga attcatccgg
      961 cgtcggcttg tttgccggag gcgcgcaccg aacggtcgcg ccgatatagc agttggtgag
     1021 agtgaggccg tcacccgcat gtatggatgt cgcctggttg gcgaacccga accggtgcag
     1081 cgcttcatac agccaatccc cactacggtc tccggtaaat acgcgtccgg ttcggttccc
     1141 cccatgtgcc gcgggcgcga ggcccaaaat gtagagcctg gcgttggaat ctccgaatcc
     1201 agggaccggc ttaccccaat aagaccactc gcggaattgg cgtcgtttat ccctggcgac
     1261 agcttctcgg taggcaacca gtcggggaca gcacgtgcag gcgacgatct cgttgttgag
     1321 cattgtcagg gcggacatag gcgcgaagtg taccaaaacc aggagatgct tgtcccccgg
     1381 cgagcttgtt gctagcatga gtcatcagtc gcgacgaaag gatgacaggg gcatgtggaa
     1441 caaagagcgg ggtgttcagg catttctagc gtgcgtcaca atgctgttcg ggctgtcttc
     1501 cgccatgccg gttttatcgg cggattcgag ttctgccgcg ttggccggcg ctaaggcgga
     1561 ggagggccgg aacgcccagg agtctgatca ggtgccggtc gcgcctccgg gcgagtcgga
     1621 gttggtgcct gagcttgaag atcgactggt catcctcccc gaaatcaaac gggagggaga
     1681 gcgcttcttc ctcagctcct tcaagttgcc cgataagctg acgttcgccg ggcaggcgat
     1741 tccgttggat aactggcagg tacgcgagcg gatcgagtac gagttctatc agttcctcga
     1801 ggatcaaggt gaaagtatca ttctggcgaa acggacaggt cgatgtt
//
LOCUS       DAORQN010000282         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1643_length_1846_cov_3.514238, whole genome shotgun sequence.
ACCESSION   DAORQN010000282 DAORQN010000000
VERSION     DAORQN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1643_length_1846_cov_3.514238"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>498)
                     /locus_tag="PK614_09805"
     CDS             complement(<1..>498)
                     /locus_tag="PK614_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82537.1"
                     /translation="LSFMMTIGAGRPAVAASLDAPVLSWDDLRRCALALGIAVAVTFL
                     NPYGWRLYQEIYESLTDRFMIETLREWQPVSFQGWAGKAYGMYLAGLACLMAGWYRRV
                     EPVRWALLLLMLGLSLRHWRNVTLFLIVSLPLVAELLALAVASCVRWVPILKTHAATA
                     LLLLT"
     assembly_gap    499..508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<509..>759)
                     /locus_tag="PK614_09810"
     CDS             complement(<509..>759)
                     /locus_tag="PK614_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82538.1"
                     /translation="RVAASQARVHRTYRLVAMVASLWVALPFLGARTQLVSLLGVAIL
                     LWGWRRVQAGQRAWVWGLPPLFLLWANLHGGFTAGLFLS"
     assembly_gap    760..804
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<805..1050)
                     /locus_tag="PK614_09815"
     CDS             complement(<805..1050)
                     /locus_tag="PK614_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82539.1"
                     /translation="MLDCLLVGFILNLALQPLVEPDLGWHLRAGLDLIAQGWRLPETD
                     PYSHTMPDWHWIEHAWLTDGLLAVIYRVMDPLSGLGLI"
     gene            complement(1097..>1602)
                     /locus_tag="PK614_09820"
     CDS             complement(1097..>1602)
                     /locus_tag="PK614_09820"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRB82540.1"
                     /translation="QGRMEWGPRALGKRSLLADPRREDMRELINSKVKCRESFRPFAP
                     SVLADRAQEFFELPAPSPYMQFTVRVKASAKGLLPAVTHVDGTARVQTVTREANQRFY
                     DLLAAFGRLTGVPVLLNTSFNVQEPIVCTPDDAVRCFQRTQVEWLVLGNLLVGRSSPP
                     AIPNHAC"
     assembly_gap    1603..1612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          413 a          617 c          494 g          257 t
ORIGIN      
        1 atggtcaaca gaagcagagc cgtcgcagcg tgcgtcttca gaatcggtac ccaccgaacg
       61 cacgaggcca ccgcaagtgc gagcaactcc gccaccagcg ggaggctcac gatcaggaac
      121 agggtgacgt tccgccaatg ccgcaacgac aaccccaaca tgagcagcag cagggcccat
      181 cgaaccggtt ccacccggcg ataccatccg gccatcaagc acgccagccc ggccagatac
      241 atcccgtagg ccttccccgc ccacccctgg aaagacaccg gctgccattc ccgtaacgtc
      301 tcaatcatga accgatcggt cagggactca taaatctcct ggtaaagacg ccatccataa
      361 ggattcagga aggtgaccgc caccgcaatg cccagcgcca gcgcacagcg gcgaagatcg
      421 tcccaactga ggaccggcgc atcgagcgat gccgcgaccg ccggccgtcc ggcgcctatc
      481 gtcatcatga aggagaggnn nnnnnnnncg gacagaaaaa gtcccgccgt aaacccgcca
      541 tgcaggttcg cccaaagcag gaacaacggg ggcagtcccc agacccatgc ccgctgtcca
      601 gcctgaacgc ggcgccatcc ccacaacaga atcgcgacgc cgagcagact gaccagttgc
      661 gtgcgcgcgc ctagaaaggg taatgccacc caaagactcg ccaccatggc caccaagcga
      721 tacgtccgat gcactctggc ctgcgatgct gccacccgcn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnngatcag acccagacca ctcaacgggt ccatcacacg
      841 gtagatcacc gccagcaggc catcggtcaa ccaggcatgc tcgatccaat gccaatccgg
      901 catggtgtgg gagtagggat cggtctccgg caaccgccag ccctgggcga tcaaatccag
      961 cccggcgcgc agatgccatc ccaggtccgg ctctaccagc ggctgcagcg cgagattcag
     1021 aatgaagccg accaaaagac aatcgagcaa gagaacgatc aggcgatgag gggaaggatc
     1081 tgaaaaacga accggttcag caggcatgat tcgggatggc cggtggagat gatctgccca
     1141 ccagtagatt gccgaggact aaccactcca cctgagtccg ctggaagcag cgcaccgcat
     1201 cgtcgggcgt acagacgatc ggttcctgca cattgaacga cgtattcagc agcaccggga
     1261 cacccgtgag tcgcccgaac gctgccagaa gatcataaaa ccgctgattg gcttcacgcg
     1321 tcaccgtctg aacccgggcc gttccatcga cgtgtgtgac ggccggaagg agtcctttcg
     1381 ccgaggcctt cacccgcacg gtgaactgca tgtagggcga tggtgccggc agctcaaaaa
     1441 attcctgcgc ccgatcagcc agtaccgagg gagcgaaggg tcgaaacgac tcccggcatt
     1501 ttactttgct gttgatcaac tcccgcatgt cttcccgccg cggatctgcc aggaggctgc
     1561 gtttccccag cgcgcgaggc ccccactcca tccggccttg atnnnnnnnn nnacgcccca
     1621 gtgtcaggtc ggcggcaacc cgctcgtaga gctgagcatc cgtcagggac tcggccagca
     1681 ggcctgcttg cgacagcgct gcccgacaag cagcttcatc gaattgtggc cccagatacg
     1741 catcgggcaa gcgacacctt cccgcctctc cttcacgcct cgccgtccac cacaaggccg
     1801 ccccaagcgc cgcacccgaa tcgcctgcgg ccggcggcac atagac
//
LOCUS       DAORQN010000283         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1650_length_1835_cov_5.696067, whole genome shotgun sequence.
ACCESSION   DAORQN010000283 DAORQN010000000
VERSION     DAORQN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1650_length_1835_cov_5.696067"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK614_09825"
     CDS             complement(<1..328)
                     /locus_tag="PK614_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB82541.1"
                     /translation="MRFAVIVLAMLLAGCASTSLPGAIHSRSYYEQGVEARTRSKGIG
                     PQLGLALAGGGIKAANFSVGVLQGLTEAGVMERVDAVSTVSGGGYAALWYFSRLLNPE
                     GNLQDVG"
     gene            complement(514..1701)
                     /locus_tag="PK614_09830"
     CDS             complement(514..1701)
                     /locus_tag="PK614_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82542.1"
                     /translation="MVLSCGTTPLPYTPDGQAVGQILGKIDVMLAQLDATSTHTVRLE
                     RIKRSLTELKSAKVGQAVAELSMKDGALDRVGWDAKPEADRRAVWEKVSAIHDELRSL
                     NAASLVSGRRYFALFSILFLLVVSGYLCVHRQVVRDWVQPPLTSHLSLAILHKARLIE
                     LQLVSLKESQNQKKKEITPPAPPVLSGQAQAPKAVEPPPMPAVSSTGQLLVPGLKPDC
                     NEPQLACLLKDLKSALYPLPLPYETIRLLGMASAELEAGDITVHVTYQNFLTHLETDL
                     ESLSTSYFWTSHPWRWLELSLWASMGCLVGLLFYIAGLLGKGVFRPEEGTMFWAELLM
                     APVVVPVIFFLFTLSGMAEFIPSQSSITMNIGVAFIFGFAIRRTIGILDIIKKRFFPD
                     PTP"
BASE COUNT          458 a          507 c          467 g          403 t
ORIGIN      
        1 cacctacatc ctgcaggtta ccttccggat tcaacaaccg tgaaaaatac cagagggcag
       61 cgtacccgcc tccggacacg gttgagactg catcgacacg ctccatcact cctgcttccg
      121 tcaatccctg aagcactccg accgaaaagt tcgcagcttt gatgcctccg cccgctaacg
      181 cgaggcctag ctgagggccg attcctttgc tgcgggttcg tgcttcaacc ccctgttcat
      241 aataagatct actatgaatg gcaccaggca gtgatgttga tgcacatccc gctaacaaca
      301 ttgcaagaac gatcactgca aatctcatcc tctgctccga gtgcacgttc ttagcttacc
      361 aatggtggct ggagcgctca aagtcttccc acatttacat agctatcggt tcgggattgt
      421 cgagagtgac acgaacttac gaagtaccat catcccgacc tttccaaacc ctacgacacg
      481 gcaacttgtc ttatcagaag atgccgctca ccctcacggc gtaggatcag gaaagaaccg
      541 ttttttaatg atgtcgagaa tgccgatggt acgacgaatg gcgaagccaa aaatgaaggc
      601 cacgccgatg ttcatcgtga tcgaggattg gctgggaata aactccgcca tcccgctcaa
      661 ggtgaacaga aaaaagatca cgggaacgac aaccggggcc atgagcaact cggcccagaa
      721 catcgtccct tcctcaggcc gaaacactcc cttcccaaga agaccggcga tatagaacag
      781 caggccgacc aggcaaccca tgctcgccca cagcgagagt tcaagccagc gccagggatg
      841 actggtccaa aaatatgacg tactcagact ttcaaggtcg gtctctagat gcgttaagaa
      901 attctgatat gtgacatgaa cggtgatgtc accggcttca agctctgctg aagccatgcc
      961 gagcaagcgt atagtttcat agggaagcgg caacggatac agcgccgatt taagatcctt
     1021 caagagacac gcaagttgtg gctcgttaca atcaggcttt agccctggaa ctaggagctg
     1081 tcctgtagat gagaccgcag gcatgggagg aggttctaca gccttgggag cctgagcttg
     1141 tccgcttaat acaggcggcg caggaggcgt aatttctttt ttcttttgat tctgcgactc
     1201 tttgagagag acgagttgta gttcaatgag gcgggcttta tgcaatatcg cgaggctgag
     1261 gtgtgaagta agtggtggtt gcacccaatc cctgacgact tgtcgatgca cacacagata
     1321 gcctgagacg accagaagga acagtatgga gaacagcgca aaataccgcc ttccactgac
     1381 aagcgaggcg gcatttaggg aacgcagttc atcatggatg gcagagacct tctcccagac
     1441 cgctcgtcgg tcggcttcag gtttcgcatc ccaaccaacg cggtctagcg caccatcctt
     1501 cattgaaagc tccgcaacgg cctgtccaac cttggcactt ttcaactcgg tcagagaccg
     1561 cttgatccgc tccagtcgaa cagtatgggt ggaggtggcg tcgagctgcg cgagcatcac
     1621 atcaatcttg ccaaggatct gccccaccgc ttggccgtct ggcgtatagg gcaacggtgt
     1681 ggtgccgcag cttagaacca tcgtcaacca cctttcgacc tttgtgacgc tactcattgc
     1741 tttgtgaccg tcgaactgag cagatcgacc aactggctat ggtcatcgat gagtgcagcc
     1801 catttctcga taacggtttt tcggtgagcg atcag
//
LOCUS       DAORQN010000284         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1659_length_1830_cov_8.575775, whole genome shotgun sequence.
ACCESSION   DAORQN010000284 DAORQN010000000
VERSION     DAORQN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_1830_cov_8.575775"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1577)
                     /locus_tag="PK614_09835"
     CDS             complement(219..1577)
                     /locus_tag="PK614_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB82543.1"
                     /translation="MTQGAHVLVVDDEVNIRGALVTLLEKKGHRVRGARSGEEALEEL
                     EAAPADLVMTDLKMPGIGGMEFLRRLKEKWPETEVVVMTAYGSIDTAVEAMRCGAYDY
                     LTKPIDRERFPIVVEKALERHALATENKQLRSRLDTRTRFDQMVGDSEPMRRVYNMVE
                     MVAESDVTVLLTGESGTGKELVARAIHHRSARANGPFITLNCGALPENLFESELFGYD
                     KGAFTGAMTTKLGRFELAHEGTLLLDEVGELSLKSQVDFLRVLETKEFRRLGGTKVIK
                     VDTRIIAATNRNLEAAVKQGDFREDLYYRLNVVPIHLPPLRERAEDVPLLVNRYLIAF
                     ATQYRREPKEVSRDAMRLLRLYAWPGNIRQLRNLMERLVVTVKDATIQPEHLPEDIQA
                     SKEDARTMLVTLGTPLEQIERDVIQRTLAEVTNHREKAAKLLGISLRALQYKIKEYGI
                     RE"
     gene            complement(1574..>1830)
                     /locus_tag="PK614_09840"
     CDS             complement(1574..>1830)
                     /locus_tag="PK614_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB82544.1"
                     /translation="GNRWVEIVVSDTGIGIEQEDLPRLFEPFYTTKSGGTGLGLAIAY
                     RIMQDHGGTIHVTSVPGNGTQVVMQFPVAVDHQQNVAVGL"
BASE COUNT          346 a          586 c          485 g          413 t
ORIGIN      
        1 ggggagaggc ctctgcaaat tctgcagcga gattccgatt ttcagcgatt cgacggctgc
       61 atttcttgca gagcgacaag tgacatcgaa tccggctgac aatcctgtgc gcgaaccgtt
      121 tcacggtccc gaatcccacc gtgggcggcc tgagcctgac cgtcctgcag catggaacct
      181 cgttcggctc tcgctgtacg gtagcgccag ggcacacgtt attcgcggat gccgtactcc
      241 ttgattttat actgcaacgc acggaggctg atgccgagga gtttggccgc tttctctcga
      301 tgattcgtca cttcggccaa tgtccgttgg atgacatccc gttcgatctg ttccaggggc
      361 gtccccagcg tcacgagcat cgtgcgggca tcctccttgc tcgcctgaat gtcctcggga
      421 aggtgttccg gttgaatggt tgcgtccttc acggtcacca ccagccgctc catcagattt
      481 cggagctgac gaatattccc gggccaggca tagagacgga gcaaccgcat ggcgtcgcgt
      541 gaaacctcct ttggctcgcg gcgatactga gtcgcgaagg cgatcagata ccggttcacg
      601 agcagcggaa catcctcggc acgctcgcgc agcggcggga ggtggatcgg gacgacgttc
      661 aatcgataat agaggtcttc gcgaaagtcg ccctgtttga cggctgcttc gagattgcga
      721 ttcgtggcgg cgatgatcct ggtatcaacc ttgatcacct tggttccgcc caggcgtctg
      781 aattccttcg tctccaagac acgcaggaaa tccacctgtg attttaaaga gagctcgccg
      841 acttcatcca gaagcagcgt tccctcatgg gcgagttcga atcgccccag cttcgtggtc
      901 atcgctccgg taaacgcccc tttgtcgtag ccgaacagtt cactttcaaa caggttctct
      961 ggtaacgcgc cgcagttcag cgtgataaac ggtccgttcg cccgcgcact cctatgatgg
     1021 atcgcccggg cgacaagttc ctttccggta ccgctttccc ccgtcagtaa caccgtgaca
     1081 tcgctctcgg ccaccatctc aaccatgttg taaacgcgcc gcatcggctc gctgtcaccg
     1141 accatctgat cgaagcgtgt gcgtgtgtcc aggcggctcc tgagctgctt gttctccgtc
     1201 gccagcgcat gacgctccag cgctttctcc accacgatgg gaaaccgctc gcggtcgatg
     1261 ggtttggtga gatagtcgta cgcgccgcat cgcatggcct ccactgccgt atcgatcgag
     1321 ccatacgccg tcatgaccac gacttccgtc tccggccact tctccttcag acgacgaagg
     1381 aactccatgc cgccgatccc gggcatcttg aggtcggtca tgaccagatc ggccggggcg
     1441 gcctcaagct cctccagcgc ttcttctccg ctccttgccc cgcgcacacg gtgacctttc
     1501 ttttcaagga gcgtcactaa cgcaccccgg atattgacct cgtcgtctac gacaagaaca
     1561 tgtgcccctt gcgtcataac cccaccgcaa cattctgctg atgatcgaca gcaacaggaa
     1621 attgcatgac cacctgtgtg ccgtttcccg gcacgcttgt cacatgaatg gtcccgccat
     1681 gatcctgcat aatccggtag gcgatcgcga ggccgagccc ggttccaccc gacttggttg
     1741 tatagaaggg ctcaaacagt cgcggcaagt cttcctgctc aataccgatg cccgtgtcgc
     1801 tcacgacaat ctctacccag cgattccctt
//
LOCUS       DAORQN010000285         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1672_length_1822_cov_3.928127, whole genome shotgun sequence.
ACCESSION   DAORQN010000285 DAORQN010000000
VERSION     DAORQN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_1822_cov_3.928127"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>279
                     /locus_tag="PK614_09845"
     CDS             <1..>279
                     /locus_tag="PK614_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82545.1"
                     /translation="HTGDALRIHVIGANSEQNGMFSVEGHEWPIEPYMPGADMISVVE
                     YAGSEILDVFIRGGAGGPYRQVGDFVWSNARLPYTQSGQWGYLRVLPT"
     assembly_gap    280..308
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            522..1511
                     /locus_tag="PK614_09850"
     CDS             522..1511
                     /locus_tag="PK614_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB82546.1"
                     /translation="MTLGTTIQSEQQAMKKSCFGWVLGLATVALASSFASSPVSAYEE
                     VTVSDGGTLTGIVKLEGTVPKPKGYNLTTLPDPFYCGRISDGEGWRILQPFNVGPGGE
                     FREVVVYLEGIEKGKPFAEKEVPQIEAKDCLFLPFTTVVRDDQSVTVVNMDPVMHDIQ
                     AYETSNLGARVLFNVPLPMNPQHPRNFKDRSEAGMYHKHMAGPPMKELVKLSKNRRIF
                     VMQCGFHAYMESWGVAITNPYFAKTDEQGRFTMTDVPPGTYKLVVWHPYIRTATEQTV
                     TIGPKGTVEATIGVPAPTGRLYANEVLDHAYTRYNVTEEAKKEIDPLVKKQDH"
     gene            1546..>1822
                     /locus_tag="PK614_09855"
     CDS             1546..>1822
                     /locus_tag="PK614_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82547.1"
                     /translation="MRIDRTISLIALATVMGVTALLAAADWAHADHELPKPPPLWSPL
                     DDVERLALIEVPAGMAQVPDGPFLMGSDPKFDRAAGPQELPQHQVYVD"
BASE COUNT          389 a          471 c          579 g          354 t
ORIGIN      
        1 cgcacacggg tgatgcgctc cgtatccatg tgatcggggc gaacagcgag cagaacggta
       61 tgttcagtgt cgagggccac gagtggccga tcgaaccgta catgccgggt gccgacatga
      121 tcagcgtggt ggagtatgcc gggtctgaga tcctcgatgt cttcatccgt ggcggtgcgg
      181 gcggaccgta tcgtcaggtg ggcgacttcg tgtggtcgaa tgcacggctg ccctacacgc
      241 agtccggaca gtggggctat ctgcgcgtgt tgccgaccgn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnca agccgaagtg ctgcccgagc cgcaggcgat tccgactgcc atgaagtaag
      361 cgggtcagtc atggcgtaac accggatggg ggagctgcct gtatcacgca gctcccccat
      421 ctgcgttttg agtctcgaga gaggaattag gtgatgggtt cacggtatga tcgatgacat
      481 ctgtcgatcg taccaagaac caatcacaga ggaggccgga cgtgacactg ggtaccacaa
      541 ttcagagtga gcagcaggcg atgaagaaga gttgtttcgg atgggtctta ggactggcta
      601 cggttgccct cgcctcgtca ttcgcgagca gtcctgtgtc cgcctatgaa gaagtcactg
      661 tatcggatgg cgggacacta acggggatcg tgaagttgga ggggacagtc ccgaaaccga
      721 aagggtacaa cctcaccaca ctgccggacc cgttctattg cgggcgcatt tccgacgggg
      781 aaggctggcg tattctgcaa ccgttcaacg tcggaccggg aggcgagttt cgtgaagtcg
      841 tcgtgtatct ggaagggatt gagaagggaa aaccgttcgc tgaaaaagag gtgccgcaaa
      901 tagaggcgaa ggattgcctg ttccttccgt ttacgaccgt cgtgcgggac gatcagtcgg
      961 tgacggtcgt caacatggac ccggtcatgc acgacattca agcctatgaa acgtcgaatt
     1021 taggcgcgcg tgtcctgttc aacgtgccgc tcccgatgaa tccgcagcat ccacgcaatt
     1081 tcaaggatcg cagcgaggcc ggcatgtacc acaaacacat ggccggtccg ccgatgaaag
     1141 agttggtgaa actcagcaaa aaccgtcgga tctttgtcat gcaatgtggt ttccatgcct
     1201 acatggagag ttggggggtg gccatcacga atccctactt tgcgaagacg gacgaacagg
     1261 gccggttcac catgaccgat gtgcctccgg gtacctataa gttggtcgtc tggcatccct
     1321 atatcaggac ggcgacggaa caaaccgtca ccatcggtcc gaagggcacc gttgaggcca
     1381 cgattggagt cccggcgccg accggacggc tgtatgccaa tgaggtcctg gaccatgcgt
     1441 atacccgcta taacgtgacc gaggaagcga agaaagaaat cgatccgctg gtcaaaaagc
     1501 aggatcattg accgatgacg gttggcgaat gacgagtgac gcacgatgcg catagatcgc
     1561 acgattagcc tgattgcgtt ggcaacggtg atgggtgtga cggccctatt ggccgcggcc
     1621 gattgggccc acgccgacca cgagttgccg aagccgccgc cgctctggtc cccgcttgat
     1681 gatgtggagc ggctggcgtt gatcgaggtg ccggccggga tggctcaggt gccggacggc
     1741 ccgtttctga tgggtagcga cccgaagttt gatcgggccg caggaccgca ggaactgcct
     1801 cagcaccagg tctatgtgga tg
//
LOCUS       DAORQN010000286         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1696_length_1781_cov_7.760718, whole genome shotgun sequence.
ACCESSION   DAORQN010000286 DAORQN010000000
VERSION     DAORQN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1696_length_1781_cov_7.760718"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..588)
                     /locus_tag="PK614_09860"
     CDS             complement(178..588)
                     /locus_tag="PK614_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HRB82548.1"
                     /translation="MTTNGSAKRIRIKVGGIQLEAELKSTRTAEELYQALPAEGPLNV
                     WGEEFYFKIPGVKDHRETATTQVKVGDVAFWGAGQVLAIFFGRTPMSMGADPVPADRV
                     NVVGRVVGDAAALRQAMNASTIRVERIEQGPSIG"
     gene            721..1494
                     /locus_tag="PK614_09865"
     CDS             721..1494
                     /locus_tag="PK614_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRB82549.1"
                     /translation="MLTSTFVHLNGIGPSTERRLWEGGIADWTTFRREPNLPGISPSR
                     KVLYDIDLTSAQEQLDRRNARYFAGCLHTRDHWRLFRTFGPRALYLDIETTGLSAHEG
                     QVTIVGLYRQGRLRTLISGDDLTLGVIQEELDQADLLITFFGSVFDIPYLQTCFKGLR
                     VSIPHFDLCFAARRVGLQGGLKHIERELDIARDSDLLTLDGMEAVRLWHQYRAGDEAA
                     LDQLVRYNAADTRNLEPLAEWLYDRLSVRYGPPSLMPTR"
     gene            1496..>1781
                     /locus_tag="PK614_09870"
     CDS             1496..>1781
                     /locus_tag="PK614_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82550.1"
                     /translation="MSTQQNRRAWTAFGQTERGLVRPTNQDTFLVENRHRLWAVADGM
                     GGQAGGDVASRLVMDSLAELAPTLGGIASAPDLHEEERATDRLTTMAEQAH"
BASE COUNT          346 a          606 c          480 g          349 t
ORIGIN      
        1 catgggcctg ctccgccatg gttgtgaggc ggtcggttgc gcgctcttcc tcgtggagcc
       61 gcgcgatcac tgattccgcc atatgatgga cccgctcttt tgccagcctc atcgcagaaa
      121 tctccccgcc gacataccat ttataccatt gccgttcaca aaacccatcc tctgagccta
      181 tccgatcgac ggcccctgtt caatccgttc aacccggatc gtgctggcat tcatcgcctg
      241 ccgcagagcc gccgcgtccc ccacgacacg acccaccaca ttgacccggt cggccggaac
      301 gggatcggcc cccatgctca tcggcgtacg cccgaagaaa atggccaaca cctggccggc
      361 gccccagaag gctacgtcac cgactttgac ctgcgtggtc gccgtttcgc gatggtcctt
      421 cactccggga atcttaaaat agaactcttc gccccagaca ttcaatggtc cctcagcggg
      481 cagcgcctgg tagagttctt cagccgttct cgtgcttttc aactccgcct ccaactgtat
      541 cccgccgacc ttgattcgaa tacgtttcgc cgatccgttc gtggtcatgt tccctcattt
      601 acaccgtgat acactgcaca actcaagcgc acgaaactcc gcaccagtcg cggactctaa
      661 cttgtttatc ccttgaaatc aaggctacaa tcccggcgga aggagatcgc gacccgcatt
      721 atgttgacct cgacattcgt ccatctgaac ggcatcggcc cgtccacgga gcggcggctc
      781 tgggaaggcg gcatcgccga ttggacgacc tttcgccgtg agccgaacct tcccggcatc
      841 tcaccctcgc gcaaagtcct gtacgatatc gatcttacct cggcgcagga gcagctcgac
      901 cgccgcaatg cccggtactt tgccggctgc ctgcataccc gcgaccactg gaggctgttc
      961 cgcaccttcg gcccacgggc actctatctg gacatcgaaa cgaccggact gtccgcgcac
     1021 gaggggcagg tgaccatcgt cggcctctac cgccaggggc gactgcgcac cctgatcagc
     1081 ggtgacgacc tgacgcttgg cgtgattcag gaagaactcg accaagccga cttgctgatc
     1141 acgtttttcg gcagcgtgtt cgatatcccc tacctccaaa cctgtttcaa gggcctgcgg
     1201 gtgtcgattc ctcacttcga tctctgtttc gccgcgagac gcgtgggcct gcagggaggc
     1261 ctcaagcaca tcgagcgcga gttggacatc gcgcgggact ccgatctgct gacactcgac
     1321 ggcatggaag ccgttcgtct ctggcaccag taccgcgcag gcgacgaggc agcgctggat
     1381 caactcgtcc gttataatgc cgccgacacc agaaaccttg aacccctggc ggagtggctg
     1441 tatgaccggc tctccgtgcg ctatggccca ccgtctttga tgccaacccg ataacgtgag
     1501 cacacagcag aacagacggg catggacggc tttcgggcag accgagcgcg ggttggtccg
     1561 tcccacgaat caagacacct tcctcgttga gaaccgacat cgactctggg cagtggcgga
     1621 cggaatggga ggccaagcgg gaggagatgt ggccagccgc ctcgtaatgg actccctcgc
     1681 cgaactggcg ccgactctcg gcggaattgc atctgccccc gaccttcacg aggaagagcg
     1741 cgcaaccgac cgcctcacaa ccatggcgga gcaggcccat g
//
LOCUS       DAORQN010000287         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1732_length_1744_cov_5.019538, whole genome shotgun sequence.
ACCESSION   DAORQN010000287 DAORQN010000000
VERSION     DAORQN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_1744_cov_5.019538"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK614_09875"
     CDS             <1..647
                     /locus_tag="PK614_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB82551.1"
                     /translation="RFLRSTLPAHGYRLYEATTGADGLAQASARNPDIILLDLGLPDM
                     NGTAVIEKIRAWSNMPIIVLSARDQEQVKVQAFDLGADDYVTKPFGVDELLARMRAAL
                     RHATRPVGDGVDASCTFGDVTVDLGRRQVFVSGTEIHLTPIEYKLLTTLVRYAGKVVT
                     HRQLLKEVWGPLHVDEGHYLRVYMRQLRNKIEADPTQPRHLVTELGVGYRLRAE"
     gene            762..>1744
                     /locus_tag="PK614_09880"
     CDS             762..>1744
                     /locus_tag="PK614_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB82552.1"
                     /translation="MKPVLLLHNDVGPAMETAPSGLLRRLWWVLLLCTLVVPAHAGEI
                     SIAPDPIPATDTDLWHYGAYLDVGYVVNPNFPENHLWRNRATAARHNEFAPNMALAYV
                     RKDTTESSRWGMELGVQGGYDSERFAFLQGEREVGGADTLRHIHRANVSYLAPVGKGL
                     TITAGLFNSLIGYESLYAKDNVNYTRSWIADNTPYMMFGVNAKYPVTDKLTVAGFIVN
                     GYYHLSHPNDQPSYGGQWAYKATPQLTLTQTVYGGPDQTNTALQFWRFYANHIVEWKG
                     DNLTFAASYDIGTENVADRPGNPRAFVMGGNVVARWHVTGPWALAVRPEFY"
BASE COUNT          360 a          483 c          512 g          389 t
ORIGIN      
        1 ggcggttcct tcgctcgacc ttgccggctc atggctatcg gctgtacgaa gcgaccaccg
       61 gcgcggatgg actggcgcag gcctccgctc gcaatcccga cattatcctg ctcgatctcg
      121 gtcttcccga tatgaacggc accgcagtga ttgagaaaat ccgcgcctgg tcgaacatgc
      181 cgatcatcgt gctttccgca cgagaccaag aacaggtcaa ggtgcaagcc ttcgatcttg
      241 gcgccgatga ctacgtcacg aaaccgtttg gcgtggatga attgctggcc cgcatgcgcg
      301 cggctcttag gcatgcgact cgtccggttg gagacggggt ggacgcgagc tgtacgttcg
      361 gcgacgtgac cgtcgattta gggcgaagac aagtgtttgt ctcggggacg gagatccacc
      421 tcacgccgat tgaatacaag ttgctgacga cgctcgtgcg gtatgccggg aaggtggtga
      481 cgcatcgtca gttgctcaaa gaagtgtggg gtccccttca tgtggacgag ggacactatt
      541 tgcgtgtgta tatgcgtcaa ttgagaaata agatcgaagc cgatcctact caaccgcgcc
      601 atttggtgac ggaactgggt gtcgggtatc gtctccgggc agaatgagtg ctccccccga
      661 ctttaataga ttctttacag ctccctgttc gattttaatc ctgttttgat acggcatgat
      721 ctaaagtcca tgtcatgaga aggtgcaacg atgagtgtcg gatgaaacct gtcttgcttt
      781 tgcacaacga tgtcggccca gccatggaga ctgctccttc cggtctgttg cgccgactct
      841 ggtgggtact tctcctctgt acgttggtcg taccggcgca tgccggtgaa atcagcatcg
      901 cgcccgatcc gattccggca accgacactg acctctggca ctacggggcc taccttgatg
      961 tcggctacgt cgtgaatccc aactttccag agaatcacct ctggcggaat cgtgcgacgg
     1021 cggcgcggca caatgagttt gcccccaata tggccctggc ctatgttcgg aaagacacga
     1081 ctgaatcatc gcgctggggc atggaactcg gtgtgcaagg cggctacgac tccgaacgct
     1141 ttgcgttttt acagggtgag cgtgaggtcg gcggggcgga tacgcttcgg cacatccatc
     1201 gcgcgaatgt gtcctatctg gctccggtcg gaaaggggct cacgatcact gccgggcttt
     1261 ttaatagcct catcggctac gaatccctct atgccaagga caatgtgaac tatacccggt
     1321 catggattgc cgacaacacg ccctacatga tgttcggcgt caatgcgaaa tatccggtca
     1381 ccgacaagct gacggtcgcg gggtttatcg ttaacggata ctatcacctg tcgcatccga
     1441 acgaccaacc gagttatggg gggcagtggg cctacaaggc gactccgcaa ctcaccctga
     1501 cccagacggt ctacggaggg ccggatcaga ccaacacggc actgcagttc tggcgcttct
     1561 acgcgaatca catcgtcgaa tggaaagggg acaacctgac attcgcggct tcctatgata
     1621 tcgggaccga aaacgtcgcc gatcgtcccg gaaatcccag ggcatttgtg atgggaggca
     1681 atgtggtcgc gcgatggcat gtgacaggac cttgggctct ggcggtccgg ccggagttct
     1741 attg
//
LOCUS       DAORQN010000288         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1735_length_1743_cov_3.347156, whole genome shotgun sequence.
ACCESSION   DAORQN010000288 DAORQN010000000
VERSION     DAORQN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_1743_cov_3.347156"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK614_09885"
     CDS             complement(<1..650)
                     /locus_tag="PK614_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HRB82553.1"
                     /translation="MSCSASTLLTSVMVVLCGVLAGCQTTAGGTTSKTGFAAGRTSLD
                     SNRCDFLPESERPDWVTGRPHTDDYVGVGQAGLSESPEQQIRASEDRARGNLAAEISV
                     KVREQLIQNLCEGQCGEEEQSKISLKAESKTKQTLKGAKIQHRWLDRGSCMLWTLVTL
                     SKDQVEMRRVMMFNLSPPTVEMAGLLVGHLEKVLREDLAVVPADAQVEGCATDSTKA"
     gene            complement(705..1457)
                     /locus_tag="PK614_09890"
     CDS             complement(705..1457)
                     /locus_tag="PK614_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82554.1"
                     /translation="MLVFLTACTVKKQVLVEVMHPPKIAIPAKVKEIDIEEFQGPVEC
                     AKSLKPKLITKVTESGVYTVAVPGLSDPEDTLMIKGTVTTCALSQGSGTLNTVFSAWY
                     MGDQVHQGVVDEHTNRPGAPPNEVRDALIDRVMSRTAKAILPMKKKEIRTFLPVDGDN
                     DTGVTAAAAGNWPLAVESFGKQVKEKPTEHRVWYNRGIAYEVIGQFKLALNDLRKAID
                     LKRSDLYVEALARVDRGMQNQKSIEDLKKNPE"
BASE COUNT          352 a          546 c          408 g          437 t
ORIGIN      
        1 gccttggtac tgtcagtcgc gcacccttcc acctgcgcat cggccggcac gacagcaaga
       61 tcctcgcgta ataccttctc aagatgtcct accagcaggc ccgccatctc aacggtggga
      121 ggcgaaagat tgaacatcat gactcgccgc atctcgactt ggtctttcga caacgtcacc
      181 agcgtccaga gcatgcagct gccgcggtcg agccatcggt gttggatctt ggcccctttt
      241 agggtttgtt tcgttttaga ttcagccttg agggaaattt tgctttgttc ctcttctcca
      301 cactgtcctt cacagagatt ctggattaac tgttctcgca ccttcacact aatttcagcg
      361 gcaaggttac cccgcgccct atcttccgac gcacgaatct gctgctcagg actctcgctc
      421 agcccggcct gccccacccc gacatagtcg tccgtgtggg gacggccagt tacccagtct
      481 gggcgttcac tttcaggtag aaaatcacac ctgtttgaat ccagcgaagt ccgacctgca
      541 gcaaatccag tcttactcgt agtcccaccg gcagtggtct ggcatccagc cagcacgccg
      601 cagagcacaa ccatcacgga ggtaagtagc gtcgaggcag aacaactcat gcccagaaac
      661 catttcattc gcctgtataa tgttcgagga atagatatca tggcttactc cggattcttc
      721 ttgagatcct caatgctctt ctggttttgc atccccctat ccacacgcgc tagggcctcg
      781 acatataggt cagaccgctt aagatcgatg gctttccgaa gatcattcag cgccagcttg
      841 aactggccga ttacttcata cgcaatcccc cggttgtacc aaactcgatg ttcagtgggt
      901 ttctctttca cctgtttgcc gaagctctcg acggccaatg gccagttgcc cgccgctgct
      961 gccgtcactc cagtatcgtt gtctccgtca acgggcagaa acgtacggat ttctttcttt
     1021 ttcatcggga ggatcgcctt cgccgtgcga ctcatgacgc gatcgatcaa tgcgtcgcgg
     1081 acttcgttcg gcggagcacc cggccgattc gtgtgctcgt ctaccacccc ttgatggacc
     1141 tgatctccca tgtaccaagc tgaaaagacc gtattgagcg taccggagcc ctgactgagc
     1201 gcgcaggttg taacggttcc cttgatcata agtgtgtcct ccgggtcaga gagacctgga
     1261 acggcgaccg tatagactcc agactcggtt accttcgtga tcagtttcgg cttgagtgac
     1321 ttcgcacact ccaccggacc ttggaattct tcgatgtcta tctccttcac cttcgctggg
     1381 atcgcaattt ttggcggatg catgacttcg accaacactt gcttcttcac agtgcaggcc
     1441 gtgagaaaaa cgagcactcc actcgccccc aagactgtgg tcagagcctt gatgacgcaa
     1501 gccgtcctgc gcctccacgc ctgtgcttgt gtcgaccctg ctagcatctc ttcccccttt
     1561 ccgatcatcc tgccaacgct cactgcatcg ccggcataca aacaagtgtg taaacagttc
     1621 caagttcccc gtgacgaatg tccttcccgc tctgcaccca ttgttcacga acctgagctt
     1681 gtttcagcag tacgtcgctg ccgaagtcag atgtttcgtt cagtcgaaca tctacccctc
     1741 ggg
//
LOCUS       DAORQN010000289         1734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1740_length_1734_cov_5.330554, whole genome shotgun sequence.
ACCESSION   DAORQN010000289 DAORQN010000000
VERSION     DAORQN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1740_length_1734_cov_5.330554"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK614_09895"
     CDS             complement(<1..445)
                     /locus_tag="PK614_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRB82555.1"
                     /translation="MLSRVASSIYWVNRYIERAENYARFIEVNLNLSLDLPRGTVEQW
                     EPLVATTGDHEMFVARYGKATKETVLHFLVADAENQNSILSCLLGARENARSIKEIIP
                     SEMWEQVNRFYLMVRDAVTRGMSHHDLHAFLADVKAASHLLWGITD"
     gene            complement(519..>1734)
                     /locus_tag="PK614_09900"
     CDS             complement(519..>1734)
                     /locus_tag="PK614_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRB82556.1"
                     /translation="IFPFDIVPRIVEAPDWTLIDTGLRQRLRALNLFIDDVYHEQKIL
                     KDSVIPSELIYSSKGFLKACWGLNPPRGIWCHIAGIDLVRISDGRYYVLEDNMRCPSG
                     VAYVLEARQVMKRTFPELFEAYRVRPVDEYPSRLLETLRSLSDLPDPTIVILTPGSYN
                     SAYYEHSLLAQKMGVELVEASDLVVIDGSVHMRTTKGSQRVDVIYRRINDEYLDPLVF
                     RPDSLLGVPGVMEAYKNGRVAIANAPGTGVADDKAIYAYVPKIINYYLAEEPILPNVP
                     TYVCWERRDRDYVLEHLDQLVVKATNEAGGYGMMIGPQASQQEREDCARRIQADPRNY
                     IAQPTLALSRSPTLVEDHIEGRHVDLRPYVLQGKGFYVLPGGMTRVALRKGSLVVNSS
                     QGGGNKDTWVLS"
BASE COUNT          368 a          499 c          504 g          363 t
ORIGIN      
        1 catcggtgat tccccacagt agatggctcg cggctttgac gtcggccaaa aatgcgtgga
       61 ggtcatggtg cgacatcccg cgagtgacgg cgtccctgac catcaggtaa aagcggttga
      121 cctgttccca catttcactg gggatgattt ccttgatcga ccgtgcgttt tcccgcgcgc
      181 ccaggagaca ggacagaatg gaattctggt tctcggcatc cgccacaagg aaatgaagca
      241 ctgtctcctt cgtcgctttt ccatagcgcg cgacaaacat ctcatgatcg cccgtcgtcg
      301 cgaccagcgg ctcccactgc tcgaccgtgc cgcgaggaag gtcaagagag agattcaaat
      361 tgacctcgat gaaccgggca tagttttccg cccgttcgat gtagcggttc acccaataga
      421 tggaactggc cactctgctc agcatcgcgg tctccgacca aggagttcgc agagccgccg
      481 gccgaccgat cggcaggagg cgtgtgcgcg cgcaagactc atgacagcac ccacgtatct
      541 ttgttgccgc caccttggga ggaattgacg accaatgacc cttttctcaa ggccacgcga
      601 gtcatgccgc ccggcaacac atagaaccct ttcccctgca agacgtacgg acgaagatcg
      661 acatgtcgtc cctcgatatg gtcttccacc agagtcggcg atcgggagag cgcgagtgtg
      721 ggctgagcga tgtagttgcg agggtcggcc tggatgcgac gggcacagtc ttctcgttct
      781 tgctgagagg cctgggggcc gatcatcatg ccgtacccac cggcttcgtt cgtcgccttg
      841 actaccaact gatcgagatg ttccagcacg tagtctcgat cccggcgttc ccagcagacg
      901 taggtgggca cgttggggag tatcggttcc tctgccaagt agtagttgat gatcttgggg
      961 acgtaggcat agatcgcctt atcatcggcg acgccggtac cgggcgcatt cgcgatcgcc
     1021 acgcggccgt tcttataggc ctccatgaca ccgggaacac ccagcagaga atcgggacgg
     1081 aacaccaacg ggtccaaata ttcgtcattg atccgccgat agatcacgtc cacccgttgg
     1141 gagcccttcg tggtgcgcat gtggaccgag ccatcgatga cgaccaggtc actggcttcc
     1201 accagttcca cacccatttt ttgagccaga agagaatgtt cgtagtaggc ggaattgtag
     1261 ctgccgggcg tgagaatcac gatcgtcgga tcggggaggt ccgacaggga ccgaagggtt
     1321 tccagcaggc ggcttgggta ttcatcgacc ggcctgacgc gataggcctc aaacaattct
     1381 ggaaaagttc gtttcatcac ctgtctggct tccagcacat acgccacccc ggacgggcag
     1441 cgcatattat cctcaaggac atagtagcgg ccgtcgctga tgcgcaccag gtcgatcccg
     1501 gcgatgtgac accagatgcc gcgaggggga ttgaggcccc agcaggcttt cagaaaccct
     1561 ttgctggaat agatcagttc gctcgggatg acgctatctt tgaggatctt ttgctcgtga
     1621 tacacatcat caataaatag gtttagtgcg cgcaggcgct ggcgaaggcc ggtgtcgatg
     1681 agcgtccaat ccggtgcctc cacaatccgc ggtacgatgt cgaagggaaa gatc
//
LOCUS       DAORQN010000290         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1741_length_1732_cov_5.324985, whole genome shotgun sequence.
ACCESSION   DAORQN010000290 DAORQN010000000
VERSION     DAORQN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_1732_cov_5.324985"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PK614_09905"
     CDS             complement(<1..612)
                     /locus_tag="PK614_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRB82557.1"
                     /translation="MKDLQVTVVGLARSGVGAARLLNHLGARVTLADRKEAQELTAIL
                     SQVDRSSIAVKVGAQYESALEGADLVVISPGVPTQLDALNRVRARGVRVIGELELASR
                     FLTAPVVAVTGTNGKSTTVTLIGKFLQESGKRAFVGGNLGIAASEAALASVQAKAGTV
                     VPYEYVVLEVSSFQLETTEQFHPWIAAILNVTLDHMDRYASVED"
     gene            complement(629..1705)
                     /gene="mraY"
                     /locus_tag="PK614_09910"
     CDS             complement(629..1705)
                     /gene="mraY"
                     /locus_tag="PK614_09910"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRB82558.1"
                     /translation="MLYNWLYPLHTQFSFLNVFRYQSFRIIYAAVTAFLIAFVMAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVVLSTLLWADMTNRYVWLVV
                     VATVGFGAIGFADDYLKFIKRQSKGLSAAQKFTCQILVALTIGVFLYTLPSYTTKLSV
                     PFFKFFTPDLGWFYIVFVILVIVGSSNAVNLTDGLDGLAIGPVMIASLAYTIVAYVTG
                     NRVMAEYLLIPYIEGAGEIAIFTGAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLLVGGVFVIEALSVILQVGSYKLRGKRIFNMAPIHHHFEMKGWDEP
                     KVVVRLWIIAILLALLSLSTLKLR"
BASE COUNT          395 a          551 c          466 g          320 t
ORIGIN      
        1 gtcctccacc gaggcatagc ggtccatatg gtccaaggtc acattcaaaa tcgcagcaat
       61 ccagggatgg aactgctcag tcgtctcaag ttggaaactg gagacttcca gcaccacata
      121 ctcatagggt acgaccgtcc ccgccttcgc ctgcacagaa gccaaggccg cctcgctggc
      181 cgctatcccg agattccccc cgacgaaggc gcgctttccg ctctcctgta gaaatttgcc
      241 gatcagcgtc accgtcgtgc tcttcccgtt ggtcccggtc acggccacca ccggcgccgt
      301 caaaaagcgg gacgccaatt ccagctcccc gatgacccgg accccgcggg ccctcacccg
      361 attgagcgca tccagttgag tcggcacacc cgggctgatc acgacaagat cagccccttc
      421 gagtgcggac tcgtactgcg cccccacctt caccgcgatg ctcgaccggt ccacctgcga
      481 caggatggcg gtcagttcct gcgcctcctt ccggtcggcc agggtgaccc gggccccgag
      541 atggttcaac aggcgtgccg cgcccactcc gcttcgggct agtccaacaa ccgtgacttg
      601 gagatctttc acgttcactg ctgttcgcct accgtaattt gagcgtgctc aagctgagca
      661 acgccagcaa aatggcaatg atccacagac gcaccacgac cttcggctcg tcccacccct
      721 tcatctcgaa gtgatggtga atgggcgcca tattgaagat gcgtttcccc cggagcttat
      781 aggatcccac ctgcaggatg accgacaacg cttcgatgac gaatacgccg ccgaccagga
      841 gcaacagcag ttcgtgcttg ctgatcacgg ccacagtacc gagggctgcc cccaacggga
      901 gcgagcccac gtcgcccatg aagacggagg ccgggtaggt gttgaaccac aaaaagccca
      961 gactggatcc caagatggct ccggtgaaga tggcgatttc tcccgcgcct tcgatgtagg
     1021 ggataagcaa atattcggcc atcacacggt tcccggtcac ataggccacg atcgtatagg
     1081 ccagcgaggc gatcatgacc ggaccgatcg ccagcccatc gagcccgtcc gtcaaattga
     1141 cggcgttgga gctgccgaca atgacaagaa tgacgaacac gatgtagaac cagccgaggt
     1201 ccggcgtgaa aaatttgaag aacggcacgc tgagtttcgt cgtatagctg ggcaacgtgt
     1261 agagaaagac gccgatggtc agcgcgacca gaatctggca ggtgaatttt tgtgccgccg
     1321 agaggccttt cgattggcgc ttgatgaact tgagatagtc atccgcgaac ccgatcgcgc
     1381 cgaagcccac cgtcgccacc acgaccagcc acacgtatcg gttggtcatg tcggcccaca
     1441 gcaacgtgga caacaccacc gcgaagataa tgagtatccc acccatggtc ggcgtaccgc
     1501 ttttggccaa atgccgcttc ggcccatcgt cgcgaatctg ctgaccgagc ttaatctcct
     1561 gcaatttgcg gatgacccag ggagccatca caaacgcgat caaaaacgcc gtgaccgccg
     1621 catagataat ccggaagctc tggtaccgaa agacgttcag gaatgaaaac tgtgtgtgaa
     1681 gggggtatag ccagttgtat aacatcctac ggcatctttg cccgccatgg at
//
LOCUS       DAORQN010000291         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1746_length_1726_cov_6.797127, whole genome shotgun sequence.
ACCESSION   DAORQN010000291 DAORQN010000000
VERSION     DAORQN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_1726_cov_6.797127"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..740)
                     /locus_tag="PK614_09915"
     CDS             complement(393..740)
                     /locus_tag="PK614_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRB82559.1"
                     /translation="MSLADNKCIPCRGGVPPLPADRTQTLLQEVGRGWSLNAQGHLER
                     LYTFKDFAQSLAFANKVGAVAEAEGHHPDLYVAWGKCTVEIWTHKINGLTESDFYLAA
                     KADREFEPFRAGA"
     gene            complement(827..1315)
                     /locus_tag="PK614_09920"
     CDS             complement(827..1315)
                     /locus_tag="PK614_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB82560.1"
                     /translation="MLVQDVMSTGVVTARRNESVRSVVTKMLSRHCGAIPVVEDGELL
                     IGMVTLRDVLIPLYPNYGEYIHDNVHSRDFVEMEEGYADVLTQRVEEVMSLNPLTVTP
                     RTPVLEAASYMGLKNFRRIPVVEKGTLVGMVSVGDINRGLFFERGHAVMDEHRVAPPS
                     GR"
     gene            complement(1506..>1726)
                     /locus_tag="PK614_09925"
     CDS             complement(1506..>1726)
                     /locus_tag="PK614_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82561.1"
                     /translation="FDEIGVKRAGFPNSGESVLGGVAGGSPMTDAKDGGDSDLARDAL
                     GEGPPRSCVDRLQGGAFFSNPTSLPYFA"
BASE COUNT          377 a          557 c          462 g          330 t
ORIGIN      
        1 tcgattcgta gagaggaatc ccgatcttgt gcgcgaatcg cacattgagg tgatgacact
       61 cgaccccgat gcccttcgct ttcaacagcg ccgacaacgt gcccaattga atggaaggat
      121 acttcgaaaa actgaagggc atgttgacga gcgccacctg ggcctgggag ctactcataa
      181 gatccccagt ccagccggca catggcgggg accaaacccg gagaagtcat ggacatcgtc
      241 acgagacagt cgagaccgaa gctcgccacg ggcgcccgca gagaggacga cgcaggcgta
      301 ttcaccgcaa tccgtcgagg aggccgagcg agaacgtccg cgccgagcga gaggatcggc
      361 tgtcgcagta gatgcgccat gatttctccg ggttaggccc ccgcccggaa cggttcaaac
      421 tctcgatccg ctttggcggc cagataaaaa tcgctctccg tgaggccgtt gatcttgtgt
      481 gtccagatct caactgtaca tttcccccag gctacataca gatccgggtg atggccctcg
      541 gcttcagcca ctgcgccgac cttattcgca aatgccagcg actgcgcaaa atccttgaac
      601 gtatacagcc gttcgagatg gccttgggca ttcagcgacc acccacgccc cacctcttgc
      661 aacaaggtct gtgtacgatc ggcggggagg ggagggaccc cgcctcgaca cggaatacat
      721 ttattgtcgg ccagactcat gacatctcct tcgcagacgg tgacagccgt cggtcgtttc
      781 aacaagaggc tattctacga gttgcccgtt cggtcccgca accgccctat cgccctgacg
      841 gtggtgcgac cctgtgttca tccatcacgg catgccctcg ttcgaaaaag agccctcgat
      901 tgatgtcgcc cacgctgacc atcccgacca atgtcccctt ctcgaccacc ggaatccggc
      961 gaaaattttt caagcccata taggacgcgg cttcaagcac cggggtccgt ggcgtcactg
     1021 tcagcggatt caggctcatg acctcctcga ctcgttgagt gaggacgtcc gcatagccct
     1081 cctccatctc cacaaaatcc cggctgtgca cattgtcatg aatatattcg ccgtaattgg
     1141 gatagagcgg aatcaagaca tcgcgcagcg tcaccatgcc gatcaagagc tcaccgtcct
     1201 cgacgaccgg aatggcgccg caatgacgac tcagcatctt ggtgaccacg ctacggaccg
     1261 actcatttcg cctggccgtg accaccccgg tagacatgac atcctggaca agcatggcag
     1321 cctcctttca cacagggcga ctcgcagagg agtcaccgga aggggaatgt attctgcatc
     1381 atcactcgct ctgatccaag tcacctccgg ccacatcatc cgatcctggc agagccggga
     1441 ccgggtccgc cgctcaccat cgtaccaact caccgggggt gagcccctcg gcgttctttg
     1501 cagacttacg cgaaatatgg tagggaagtg gggttcgaaa agaaagcccc tccctgcaac
     1561 cggtcaacgc aggatcgggg agggccctct cccagggcat ctcgcgcgag gtcgctatcg
     1621 ccaccgtcct tcgcatcggt catcggggag ccgccggcca cgcccccgag aacactctcg
     1681 ccgctatttg gaaagcccgc tcgtttcacg ccgatctcat cgaaat
//
LOCUS       DAORQN010000292         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1751_length_1723_cov_4.669065, whole genome shotgun sequence.
ACCESSION   DAORQN010000292 DAORQN010000000
VERSION     DAORQN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_1723_cov_4.669065"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PK614_09930"
     CDS             complement(<1..412)
                     /locus_tag="PK614_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB82562.1"
                     /translation="MMPTLPIIRPTLPSLDEFVGMIRPSWESGAVTNGAMVKALEAEA
                     CRQTGARHAVAMSSCTSGLILVPRALNLPPGGEVIVPSFTFTATAQALLWNGLVPVFC
                     DCLPGTCTLDPVDVERNLSERTVAICAVSIYGLPP"
     assembly_gap    601..610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(641..1525)
                     /locus_tag="PK614_09935"
     CDS             complement(641..1525)
                     /locus_tag="PK614_09935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRB82563.1"
                     /translation="MVTTLRLFSGLPRLIRPLSTMDSLSARAIIRPATVDDSHAIASI
                     MQIGASKQIHRITIIGSPRLHCHIRQQIHTADSDEYLVGSLQGHIVGMAAWGHSDGTL
                     MLNHIFLLPGFRGRGIGKSLVVDGLRRFHRNHEHLVGVDVFAANPRARSWYDSMGMQR
                     EFERSWVQIPLDSISSPISEGWTASNIPQADLDHTRVGFSQFRLRTPVRTYSIGRLGG
                     SLFRCAGFDILNDLCALSALVTLDRARDLLCIDRSESLTPDIAAKGRVVARSERMTAS
                     TDALLDVLSSGAKPVSHP"
BASE COUNT          400 a          481 c          503 g          329 t
ORIGIN      
        1 ccgggggcaa cccatagata gacactgcac agattgccac tgtccgttcc gacaagttcc
       61 gttcgacatc gactggatca agcgtgcatg ttcctgggag acagtcgcaa aataccggga
      121 ccagaccatt ccacagcagg gcttgcgccg tggccgtaaa ggtaaaggac ggtacgatga
      181 cttctccgcc aggaggaaga ttcagggcgc gagggaccag aatcaagcca ctggtacagg
      241 aagacatcgc cacagcgtgg cgtgcccccg tctgacggca ggcttcagct tccaaggcct
      301 tcaccatagc gccgttggtg acggctcctg attcccagga gggacggatc atgccaacga
      361 actcatcaag cgaaggtagg gtcggacgaa taatcggcaa ggtgggcatc atagtgtttc
      421 tccctaaatg aacagtttcg tggtggctga gactcttcgc ctccaactag tcagtgaagg
      481 cgttacgatc gttcgtctgg aaactgagga tgattcggcc tagcatcgga acccgctccc
      541 tcaactcttc tttgaggaag gaggtcatta cgtccggtta tggatgcaga gtgtgacttt
      601 nnnnnnnnnn aatgatgtgg ggctgagaac ggtgcagaaa ctacggatgg gagacaggct
      661 ttgctccgga cgacaggacg tcgagcagcg catccgtaga cgccgtcatc ctctcgctgc
      721 gtgcgacgac gcgtcccttg gccgcgatgt cgggcgtaag actctccgac ctgtcgatgc
      781 acaacaggtc gcgagcgcga tcaagcgtca ccaaggcact gagtgcgcaa agatcgttca
      841 ggatgtcgaa accggcgcag cgaaacaggg acccaccgag gcggcctatt gaataggtgc
      901 gaaccggagt gcgcagcctg aattgggaaa atccgacccg cgtatggtca agatcggcct
      961 gagggatgtt gctggcggtc catccctcag aaataggcga ggaaatggag tcgagaggaa
     1021 tctgcaccca agacctctca aattctcgct gcatgcccat ggagtcgtac caggatctgg
     1081 cgcgaggatt cgcggcaaat acgtccacac ccacaagatg ttcatggttg cgatggaacc
     1141 ggcgcagccc atccacgacc aaagactttc caatgcccct ccctcgaaac cctggcagca
     1201 ggaaaatgtg attcagcatg agggtaccgt ccgaatgtcc ccaggcagcc ataccaacga
     1261 tgtgtccctg aagcgagccg accagatact catccgaatc cgcggtgtgt atctgctgtc
     1321 ggatatggca gtggagtctt ggactcccga tgatggtaat gcggtggatc tgtttgctgg
     1381 ctccgatctg catgatcgag gcaatcgcgt ggctgtcgtc aaccgtggca ggtctgatga
     1441 tggctcgagc ggatagagaa tccatagtcg agagcgggcg gatcagccgc ggtaggccgg
     1501 aaaatagtcg cagggtggtg accactgatt tccctgccgg cgctcccaag ctgcacgtat
     1561 gtcaataagg gcctcatacg aagtgctgtc gaaaattcta gtcccaacgc taaaggcaac
     1621 acctccattg ggcgcatatc gctgcttgta gcgaagaaga ctgtcctgtc cagaataccc
     1681 ccccccctac gcccccccca cccgtgccag acgcgcggcg cgg
//
LOCUS       DAORQN010000293         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1763_length_1713_cov_10.020507, whole genome shotgun sequence.
ACCESSION   DAORQN010000293 DAORQN010000000
VERSION     DAORQN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1763_length_1713_cov_10.020507"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK614_09940"
     CDS             complement(<1..411)
                     /locus_tag="PK614_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82564.1"
                     /translation="MPELKTRLHWLMGLRVVVVTLMLGLSLAFQSTRGELVPTFSALI
                     ILTYATTIVYALLLRRLSAPAALTAFTWGQVGLDFVLETVLVARTGGIESPFAVLYVI
                     TVTVASLVPHRRLGLVTGARCTLLFGLVPAVHYLG"
     gene            complement(618..>1713)
                     /locus_tag="PK614_09945"
     CDS             complement(618..>1713)
                     /locus_tag="PK614_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRB82565.1"
                     /translation="LEEKSGMGGKFKFSLGSGLTDKDLVVFTRQFGTMINAGLPLIQC
                     LDILSTQSENKVLRETVGDVKNSVEAGSTFSDALKRHPKVFDDLYVNMIHAGEVGGLL
                     DTILTRLAKHIEKAMKLKGQIKSAMVYPTAIVGVAVIIISVLMVWVIPVFAQMFLEMS
                     GGKVGLPGPTQIVINVSNFFQSYWYAMGGAMVAAAIAIKRYYATVNGRVVIDRLLLKV
                     PIVGDLIRKASVAKFTRTLGTLITSGVPLLEGLSICAKTSGNKVIEEALMNARVSISG
                     GKTISEPLAKCNVFPKMVTHMIAVGESTGALDAMLGKIADFYEDEVDQAVETLTSLLE
                     PIMMVVLGTIIGFIVIAMYLPIFTMAQAIQ"
BASE COUNT          380 a          513 c          493 g          327 t
ORIGIN      
        1 ccctaaatag tggaccgccg ggaccagacc gaagaggagc gtacaacggg cgccggtaac
       61 cagccccagg cgtcgatgcg ggacgagact ggcgacggtc accgtaatga catagagcac
      121 cgcaaaggga ctttcgatcc ccccggtcct cgcgaccagg acagtctcca ggacaaaatc
      181 cagccccacc tgcccccatg taaacgccgt gagcgcagca ggggcgctca gccgtctcag
      241 taagagcgcg tagacgatgg tggtggcata ggtgaggatg atcagggcgg agaaggtcgg
      301 caccagttcg ccgcgggtgg actgaaacgc cagggacagc cccagcatca gcgtcaccac
      361 gaccacacga agccccatga gccaatggag tctggtcttc agctcaggca tcgagcgctc
      421 gcccggcgac gtccggtacg gatctgtcag tggcgatgtc tgggaggtcg agctggagga
      481 cggctcagca ccggtggttc gaactggaag ggtcgaggac agcatggggg gcgcgttgcc
      541 ctgatggatg tgcagcgcat ggcccgcttc gaagatcggg ccatgcgccg gagaacggac
      601 gcgacggtgc agcgtcgcta ttggatggcc tgcgccatcg tgaagatcgg aagatacatc
      661 gcaatgacga taaatccgat gatggtaccc aacaccacca tcatgatcgg ttcgagcagc
      721 gaggtcagcg tctcgaccgc ctgatcgact tcgtcctcat aaaagtcggc gatcttgccc
      781 agcatggcat cgagcgcgcc ggtggactcg ccgaccgcga tcatgtgcgt gaccatcttg
      841 ggaaacacgt tacacttggc caacggttcg gagatcgttt ttccgccgct gatgctcacc
      901 cgcgcattca tcagcgcttc ctcgatcacc ttgttgccgg aggtcttggc gcaaatgctc
      961 aacccctcga gcaacggcac accgctggtg atcaaggttc ccagagtgcg ggtaaacttc
     1021 gcgacggagg cttttctgat cagatctccg acaatcggaa ccttgagcaa cagccggtcg
     1081 atcaccacac gcccgttcac ggtggcatag taccgcttga tcgcaatggc cgcggccacc
     1141 atggcaccgc ccatggcata ccagtagctc tggaaaaaat tactcacgtt gatcacgatc
     1201 tgggtcggac caggcaatcc gacttttccg cccgacattt ccagaaacat ctgggcaaac
     1261 acgggaatga cccacaccat caagacactg atgatgatga cggccacccc gacgatcgcg
     1321 gtgggataga ccatggccga cttgatctgc cccttcagct tcatcgcctt ttcgatgtgc
     1381 ttggccagac gagtcagaat cgtatcgagc agaccgccga cctcaccggc atgaatcata
     1441 ttcacataga ggtcgtcgaa cactttcggg tgtcgcttca acgcatcgga gaatgtggat
     1501 cctgcttcca cgctgttttt cacatcgccg accgtttctc gcagcacttt attttcggac
     1561 tgggtggaga gaatatcgag acattggatc aggggcaatc cggcattgat cattgtgccg
     1621 aattgacggg tgaagaccac gagatccttg tcggtcaggc cgctccccag gctaaacttg
     1681 aactttccgc ccatgcccga cttttcttcc agg
//
LOCUS       DAORQN010000294         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1765_length_1710_cov_5.016918, whole genome shotgun sequence.
ACCESSION   DAORQN010000294 DAORQN010000000
VERSION     DAORQN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_1710_cov_5.016918"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PK614_09950"
     CDS             complement(<1..957)
                     /locus_tag="PK614_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRB82566.1"
                     /translation="MLGIVREWSVYFVLLCAASSPPVWTAVAFAEEPLEPDPVVQAEE
                     VVVSATKTPLPVSQVTSAVEVITAQDMKKQNIRNVADALRLAQGLAVFSNGGPGTEVN
                     ARIRGGSSSQTLVLIDGAIVNSGTLGSYNFANLTTDNIERVEVLRGAQSMLWGSDAMG
                     GVINIVTKRGQGPLSATGFMEYGSFASLREGGMVSGKQGAVDYSFSLSRWDTSSFSAV
                     NYRRGAGERDSYRNWQGSGRIGVDLPKDGRLDFTMRWMNSDVQLDSVSATSPSDVYGS
                     KNRSQEYVFSGSYEQPLTGWWSQKLTLARAQEASQFLSGTLQR"
     regulatory      complement(985..1247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1311..>1710)
                     /locus_tag="PK614_09955"
     CDS             complement(1311..>1710)
                     /locus_tag="PK614_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB82567.1"
                     /translation="SRAVTDLSGGERQRTMIARALAQTPRVLLLDEPTAFLDLQHQLE
                     ICSVLRRLTDEQGLTVVIVSHDLNLASQYCDRIVMLKEGSMYSMGTPAEVLSVEVLRA
                     VYGCEVLIDPHPESGLPRITLPRQAVPFRG"
BASE COUNT          367 a          560 c          448 g          335 t
ORIGIN      
        1 acgctgcaac gtgcctgaca aaaactgcga ggcctcctgt gcacgcgcaa gcgtgagctt
       61 ctgggaccac cagccggtga gcggctgttc ataactaccg ctgaacacat attcctgact
      121 ccgatttttt gagccataga cgtcgcttgg agaggtcgcc gatacactat cgagttgcac
      181 gtcggaattc atccagcgca tcgtgaaatc aagacggccg tcctttggaa ggtctacacc
      241 gattcgccct gatccctgcc agttacgata ggagtctcgc tcgccggcgc cacgccgata
      301 attgacggcc gaaaaactcg aggtgtccca gcgggacagc gagaagctgt agtcgaccgc
      361 gccctgtttc ccggacacca tgccaccttc acgcagggag gcgaaggatc cgtattccat
      421 gaatccggtg gcggagagcg gaccctggcc gcgcttcgtc acgatgttga tgacgccccc
      481 catggcgtca gacccccata acatactctg cgccccgcgc aaaacctcaa ctcgctcgat
      541 attgtcggtg gtcagattgg cgaagttata actgccgaga gtcccgctgt tgacgatggc
      601 cccgtcgatc aacactaacg tctggctcga actgcccccg cgaattctcg cgttgacctc
      661 agtccccggc cctccattgg agaacactgc cagtccctga gccaatcgaa gggcatcagc
      721 cacgtttctg atgttttgtt ttttcatgtc ctgggcggtg atcacttcca ccgcgctggt
      781 cacttggctc accggcaacg gagttttggt tgccgagacc acgacctcct cggcttgcac
      841 cacgggatca ggctccagag gctcctcggc gaatgcgacg gccgtccaca caggtgggga
      901 acttgcggca cacaacaaca cgaaatagac agaccattcc ctgacaatac ccaacatact
      961 gacactccct ttcgctcgaa gggttcagtt acaaacccgt cagttgggtc tcctgacttg
     1021 cggctcatcg tttctctgcg ccttcccagc tgagcgtgaa acgtaaaacg acgttcgtga
     1081 aacggtcgga gggaacctcc tgcgcttcac gagatacgat tcacgaacca cgccactcgc
     1141 cagtggctca atgcagagtc actccccgct tacagtggcg gcaccgtgat ggttttgcac
     1201 catcttcccc gacgctgacg gcgtcttcct gacactccgc tctccaatga tgacgcgccc
     1261 cttctcttca cacgggtggg ccgggcacga acccatcccg caacacgtga tcatcccctg
     1321 aacggcacag cctgcctcgg caaggtaatc ctgggcaatc ccgactccgg atgcggatcg
     1381 atcaagacct cacagccata gaccgcccgc aatacctcca ccgacaacac ctcagccggc
     1441 gtgcccatcg aatacatcga cccttccttc agcatgacga tccggtcgca atactggctg
     1501 gccaggttca aatcgtgcga cacgatgacg acggtcaggc cctgctcgtc ggtcaaacga
     1561 cgcagcaccg agcagatctc gagttgatgc tgaagatcga gaaaggcggt cggctcatcg
     1621 agcaagagga cccggggcgt ttgggccaag gcccgggcga tcatcgtacg ttgacgttca
     1681 ccgccggaca ggtcggtcac cgcacgcgaa
//
LOCUS       DAORQN010000295         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1786_length_1696_cov_3.137721, whole genome shotgun sequence.
ACCESSION   DAORQN010000295 DAORQN010000000
VERSION     DAORQN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_1696_cov_3.137721"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1268
                     /locus_tag="PK614_09960"
     CDS             327..1268
                     /locus_tag="PK614_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRB82568.1"
                     /translation="MNSSQSIKSLQDNIAQVIKGKPQVIEMTVVCLLARGHLLIEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGISMFNRQKQAFEFMPGPLFANIV
                     LADEINRTTPKTQSSLLEAMSESQISVDNQTYPLQQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPTPDEEKKVLDRPQLLHPADEIQPVLSAQHIVDLQAQAEKVRMED
                     SLMDYLLAIVLATRHNELLSLGVSTRGALALCRAAKALALVRDRAYCLPEDIKELAPT
                     VLSHRVMLSRSQGMRTNTVEHTERVIQDILETVPVPV"
     gene            1346..>1696
                     /locus_tag="PK614_09965"
     CDS             1346..>1696
                     /locus_tag="PK614_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82569.1"
                     /translation="MATNNLQALFRRLFRDRTIRVTTEGIRFLLLTLAVGIAAVNTGN
                     NLFYLLLAMMLSLVVLSGLLSEQCVRRLDVHRHLPDYLFVNQPTTATLRVANHKSRLP
                     SVSLRLLDVVAGKDL"
BASE COUNT          356 a          572 c          433 g          335 t
ORIGIN      
        1 cctgccggcc ggaccgcagc ctaccgattc cgacaagtga ccattcagcg cggcgctctg
       61 ttcgaaggtc gtccagccag cagcacggcc cggcggtttc aggggatggc ggaagaaact
      121 cccccctggt ttcttactct cgttcctgaa ccagtccaga cggcgtgaca ggctcacaag
      181 agtcgcgcca gcgtggcaat ccctacagct tccgcctggc ctcccgcgtg aaagtccgta
      241 taaattgacg gtctccagac cctttgttag acttcgggca taacgcccct ggtgccgacc
      301 gtaaccctct tcagaaacat cccatcgtga attcttctca atctatcaaa tcgttacagg
      361 acaatatcgc gcaggtcatc aaagggaaac cacaggtgat cgagatgacg gtcgtctgcc
      421 tgctggcacg cggccatctc ctgatcgagg acgtccccgg tgtcgggaag accacgttgg
      481 cccatagcct cgcgcgctcc ctcgattgct ccttcaaacg tattcagttc actagcgacc
      541 tgctgccctc cgatatcgtc ggcatttcca tgttcaatcg ccagaaacaa gcgtttgagt
      601 tcatgccggg cccgctgttt gccaacatcg tcctggccga cgaaatcaac cgcaccacgc
      661 cgaagactca aagtagcctc ctcgaagcga tgagcgaatc ccagatctcc gtcgacaacc
      721 aaacctatcc ccttcagcag ccgtttatgg tcatcgccac acaaaacccg gcagagtatc
      781 acggcacctt cccgcttcct gaatcgcagc tcgaccgatt cctcatgcga cttcgcatcg
      841 gctatccgac tccggatgag gaaaagaaag tgctggatcg cccccagctg ctgcacccgg
      901 ccgacgaaat tcaaccggtc ctgagcgcgc agcatatcgt cgatcttcaa gcgcaggcgg
      961 aaaaggtccg gatggaagac agcctgatgg attatctcct cgccatcgtg ctcgccacca
     1021 ggcacaacga gctgctgtca ttaggcgtga gtacccgagg cgccctcgcg ctgtgcagag
     1081 cggcgaaagc cctggccctc gtgcgcgacc gcgcctattg cctccccgaa gacatcaaag
     1141 agctcgcacc aaccgtcctc tcgcaccgtg tcatgctcag tcggtcccag gggatgcgca
     1201 cgaacaccgt cgaacatacg gaacgggtca ttcaagacat tctagagaca gtcccggttc
     1261 ccgtgtaaca accgccaggg ggccgaacac cggcattacg cgaactgtcg cagtaatgcg
     1321 gtctggccgg cagcagtcca tcagcgtggc aaccaataac ctccaggctc tgttccgccg
     1381 gctgttccgg gaccgcacca tccgggtgac aacagaaggc atccgtttcc tgctgttgac
     1441 cctggccgtc gggatcgccg ccgtcaacac cggcaataat ctgttctacc tcctgctggc
     1501 catgatgctg agcctggtcg tactgtccgg gttgctgtct gaacaatgtg tgcggcgact
     1561 ggacgtccat cggcacctcc cggactacct cttcgtcaat cagccgacca ccgcaacgct
     1621 ccgggtcgcc aatcacaaat cccgtctccc gagtgtctcg ttacggctcc tcgatgtcgt
     1681 tgccgggaaa gatctc
//
LOCUS       DAORQN010000296         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1809_length_1665_cov_5.352174, whole genome shotgun sequence.
ACCESSION   DAORQN010000296 DAORQN010000000
VERSION     DAORQN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_1665_cov_5.352174"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK614_09970"
     CDS             <1..205
                     /locus_tag="PK614_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB82570.1"
                     /translation="LEDALRIMRQAIVKAPDLPILNYHLGAALYQSGRNSEARAYLAK
                     ALKSTEQFQGRREAQQLLARTNG"
     gene            218..829
                     /locus_tag="PK614_09975"
     CDS             218..829
                     /locus_tag="PK614_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRB82571.1"
                     /translation="MQLSFKIVQRVMLGLIVGLFLTTTMSCQSADVRGTSLEVLNSEQ
                     GYQLGPEDVLLISVWKDEHLTREVVVRPDGMVSFPLVGDVPAEGRTVEELRIDLAKRL
                     IKYIPAVNITVAVIKPLSYKVYVVGRVAKPGEFLIGHYTDVLQALSLAGGLTPFAAEN
                     DIKVVRRVLGQQQTFPFRYGDVRKGIDLDQNIILQRGDVVMVP"
     gene            977..>1665
                     /locus_tag="PK614_09980"
     CDS             977..>1665
                     /locus_tag="PK614_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82572.1"
                     /translation="MSTKTYFNDNLLLTPLPHDPTYGYWISPGVEFAGKTERLEVSGK
                     AALDFVDYYGGETTRFTNVFLPLTLKYRTERDEWGFTGGFTRDNTLMGEFLTTGIVLR
                     FTQRNLWNLNPTWTRMITEKMAFQGSFQFSDASYQDGLRLGLIDYQVYGGSAGLLYHV
                     TERDDVQIAGTYTNFHTTNAPFGLRAYYPGVMLSVTHAFTEGLKATAYGGPRFVSSTN
                     QIGGFSQNTNDT"
BASE COUNT          396 a          418 c          462 g          389 t
ORIGIN      
        1 tctcgaagat gcgcttcgca ttatgcggca agcgattgtc aaggccccgg atctccccat
       61 acttaactat caccttggcg cggcgctcta tcagtctggc cgaaattccg aagccagggc
      121 gtatcttgca aaggcactca agagcacaga acaattccaa gggcgacgtg aagcgcaaca
      181 gctgctcgcc cggacgaacg gataggagtc atcaaccatg cagctgagtt tcaagattgt
      241 tcaaagagtc atgttgggat tgatcgtagg actgttcctg accaccacta tgagttgtca
      301 atccgctgac gtgcgcggca cttccctgga agtactcaat tccgaacagg ggtaccagct
      361 cggccccgaa gatgtcctgc tcatttccgt ctggaaagat gaacacctga caagagaggt
      421 ggtcgtccgg ccggatggca tggtttcttt tccactggtt ggagatgtgc cagccgaagg
      481 acgaacggtg gaggagctgc gcatcgacct tgccaaacga ttgatcaaat atattcctgc
      541 cgtgaacatc acagtggccg ttatcaagcc gttgagttac aaggtctatg tcgtcgggcg
      601 ggtagcgaag cccggtgaat tcctgatagg tcactatacc gatgtcctcc aagccttgag
      661 cctcgccggc ggattgaccc cctttgcggc agaaaacgat attaaagtcg tgcgccgggt
      721 tttagggcaa cagcagacat ttcccttccg gtacggtgat gtccgtaagg ggattgatct
      781 ggatcagaat atcattttgc aacgcggtga cgtagtgatg gtgccatagt aggtgaagag
      841 gtcactattt tacagccttg agggtagagg aaagcgctgg agggcaaaga cgggtatttt
      901 gtgcctactg ctcgtcgccg tcttcgcatc ttcagcttct gcaggcctcg cagcggaatg
      961 gtcacttgtg ccttcgatga gtaccaagac ctatttcaac gacaacctct tgctgactcc
     1021 gttgccgcac gatccgacct atggttattg gatctcgcca ggagtggaat ttgccggcaa
     1081 aactgaacgg ctggaggtga gtgggaaggc ggcattggat tttgtggact actacggtgg
     1141 agaaaccact agattcacga atgtattcct gccgttgacg ctgaagtacc ggactgagcg
     1201 ggacgaatgg ggattcaccg gtggattcac cagagacaac actttgatgg gagaattctt
     1261 gacgaccggc atcgttttac gatttaccca gcgaaacctc tggaacttga atccgacctg
     1321 gaccagaatg attacggaga aaatggcctt tcaagggtcg tttcaattca gcgacgccag
     1381 ctatcaggat gggctgcggc ttgggttgat tgactaccaa gtctacggcg gctcagctgg
     1441 gctcctgtat cacgtcacgg aacgtgacga tgtgcaaata gctggaacct acacgaactt
     1501 ccatacgacc aatgctccat ttggtctaag agcctattac ccgggagtca tgttatccgt
     1561 cacccacgcc ttcaccgaag gattgaaggc gacggcctat ggtggaccgc gttttgtgag
     1621 ttccactaac cagatcggcg ggttcagtca gaacacgaat gatac
//
LOCUS       DAORQN010000297         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1824_length_1651_cov_5.631579, whole genome shotgun sequence.
ACCESSION   DAORQN010000297 DAORQN010000000
VERSION     DAORQN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1824_length_1651_cov_5.631579"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK614_09985"
     CDS             complement(<1..535)
                     /locus_tag="PK614_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82573.1"
                     /translation="MHVDSDNLAAFRKQITSARSLDAQAWEASRHLVNAAHWPMTLQA
                     LVNAIETSTVPDTIKRSLVEALPPEHESTNRIASTESLKHLTGLPPSKALRALCIFFG
                     VRSKPSSKWPLPAVTADTIDVLIRRHHNPFDLLTQENPASVLDLGAGDLSFAEELATL
                     YEPQLAAQSRPLIFHCLD"
     gene            675..>1651
                     /locus_tag="PK614_09990"
     CDS             675..>1651
                     /locus_tag="PK614_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRB82574.1"
                     /translation="MNTFTPSLFRKTRPVLGRLLALLGLILMMFGCQAKDRSAMVTHR
                     VWDEWAVGQVVDARTGLAVPMQSWLEGLVTYDVIYLGEEHHNRSHIDAALTVLRSLMS
                     QGRRPWLAMEMFGWDGQPALARYLRSEESIRSEFLEQVAWKQNWGGAFEDYEPLAQFA
                     KDQRLPLLAMNPPKSLIRQVVKQGLVQAKEQPEWRQWGMEGEVIVDAPAYRSRILSQL
                     QDCHGGGSPEDYQTMYEASMVRDEGMAKTLAAAMKQLRVEASSGQGPLLSYTGGGHVQ
                     YRLPVPDRVARRVPEGLKQVTVYMATFERERAAELHRAMQEGIADYVWLT"
BASE COUNT          316 a          412 c          567 g          356 t
ORIGIN      
        1 ggtcaaggca gtggaaaatg aggggtctgc tttgcgcggc tagctgaggt tcgtacaggg
       61 tggccagctc ttcggcaaac gaaaggtcac cggctcccag atccaagacg gaagccgggt
      121 tctcttgtgt cagcaggtcg aaggggttgt ggtgccggcg gatcaatacg tcgatggtgt
      181 ctgccgtgac cgccggtaac ggccatttcg atgatggttt cgagcgcact ccaaaaaaga
      241 tgcaaagcgc ccgtaaggct ttggagggcg ggagtcccgt caggtgcttg agcgattccg
      301 tggaggcgat gcggttcgtg ctctcgtgct ccggcggcag ggcttcaaca aggctgcgtt
      361 tgatcgtatc cgggacagtc gaggtttcga tcgcgttgac cagcgcttga agggtcatcg
      421 gccagtgcgc ggcattcacc aggtgacgcg aggcctccca ggcttgtgca tccagtgacc
      481 gcgccgacgt gatctgtttc cgaaaggccg ccaggttgtc agagtcaacg tgcatgattc
      541 gatcagcggt gtacggattc ctcggtggtg cggggatgaa agctgctgcg tgtctgggca
      601 gagtatcggg cctgtcgtaa gggagtcaag ggagctgcct tgcggctact gggtccgtat
      661 gttaggtaga ccatatgaat acattcactc catcgctttt ccggaagact cgcccagtct
      721 tgggccgctt gctcgccctg cttggtctta tcttgatgat gttcggctgc caggcgaaag
      781 atcgctcagc gatggtcact catcgggtct gggacgaatg ggcggtgggc caggtggtgg
      841 atgccaggac cggtctcgca gtaccgatgc agtcgtggtt ggaggggctg gtcacgtacg
      901 atgtgatcta cctgggagaa gaacatcaca atcgatcgca tatcgacgcg gcgttgacgg
      961 tgcttcgctc ccttatgagc caggggcgtc gtccgtggct cgcgatggaa atgttcgggt
     1021 gggacggtca gccggcgctc gctcgctatc tgcgttcgga ggagagtatt cgctcggagt
     1081 tcctggagca agtcgcgtgg aagcagaatt ggggcggcgc gtttgaagat tacgagccat
     1141 tggcgcaatt cgcgaaagat caacgtcttc cgcttctggc aatgaatccg ccgaagagtc
     1201 tgatccggca ggtggtgaag cagggtttgg tccaagccaa ggaacagcct gagtggcgtc
     1261 aatggggcat ggagggagag gtcatcgtgg atgcgcctgc ctaccggtcc cggattttat
     1321 ctcaactcca ggactgccac ggcggtgggt ctccggagga ctaccagacc atgtatgaag
     1381 cctctatggt cagagacgag gggatggcga agaccttagc agcggcgatg aagcaactac
     1441 gtgtcgaggc cagctccggt cagggccctc tgctcagtta tacgggcgga ggtcatgtgc
     1501 aataccggtt gccggtcccg gatcgggtgg ctcgacgagt tccggaaggg ctcaagcagg
     1561 tcacggttta tatggccaca ttcgagcgag agcgggctgc agagcttcat cgggccatgc
     1621 aagaagggat tgctgactat gtgtggctca c
//
LOCUS       DAORQN010000298         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1835_length_1638_cov_4.135186, whole genome shotgun sequence.
ACCESSION   DAORQN010000298 DAORQN010000000
VERSION     DAORQN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_1638_cov_4.135186"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK614_09995"
     CDS             complement(<1..250)
                     /locus_tag="PK614_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82575.1"
                     /translation="MDFTGLNQFDHDTARHINAISLQLTHEPPFRPILLELANQYLAF
                     AIPDEDCLTLDIIDKLELLGGSAELNFSGLVHLSSANPE"
     gene            364..1101
                     /gene="cas5c"
                     /locus_tag="PK614_10000"
     CDS             364..1101
                     /gene="cas5c"
                     /locus_tag="PK614_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HRB82576.1"
                     /translation="MSKSYCLEVSGDFACFTRPEMKVERVSCDVMTPSAARAVFEAIV
                     WKPAIRWRVIKIEVLKPIKWISVRRNEVGAVVSTRNVQAAMSAGSGELGLHVEDERQQ
                     RAGLFLRDVTYRIHAYFELRDPSFHKSHSTQLSKVPVNHEEDDLLALSDNNATKFMSM
                     FERRASRGQCVNQPYLGCREFACSFRFIEDVKAEPKPIEETKDLGWMLYDLDYSNPAD
                     PKPRFFKARLDNGVVMVPAWESEEVRG"
     gene            1098..>1638
                     /locus_tag="PK614_10005"
     CDS             1098..>1638
                     /locus_tag="PK614_10005"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HRB82577.1"
                     /translation="MILQSLHDYYQRKPDLPREGFEEKEIPFIIEIDRNGVAAQIEDT
                     RTQDGKRKRAKKSVNVAANLLWGNAEYVLGLPDSKKLGEQKGKGKELEYQARLKEMRG
                     AFIKEIASLPPPCQTDPGIQAVLKFCEQSDFTDLARFGETWKDVQASNQSTTFRLQGD
                     TGLVCQRQAVIEAVSKSVTK"
BASE COUNT          425 a          390 c          457 g          366 t
ORIGIN      
        1 attcaggatt tgctgacgac agatgcacga ggcctgaaaa attcaattcc gcactgccgc
       61 cgagaagttc aagcttatcg atgatgtcga gagtcaagca atcctcgtct ggtatggcga
      121 aagccaaata ttgattggca agctcaagaa ggatgggccg gaacggtggc tcatgcgtaa
      181 gctgcaacga tatagcgtta atatgcctcg ctgtgtcgtg atcaaactgg ttgagaccgg
      241 tgaagtccaa gaggtttggc ctggcatctt cgcgcaggca gtaagcactt tatatgacga
      301 taagctcggc gtgatcgtgg atggcgcact aaatgctgaa caactgattg ggtgagggac
      361 tcgatgagta aatcttattg tcttgaagtg tcgggcgact tcgcctgctt tacgcgccct
      421 gagatgaagg tggagcgcgt ttcatgtgac gtgatgacac catcggctgc tcgagcggta
      481 ttcgaagcta ttgtctggaa gcctgccatc cgatggcgcg tgattaagat tgaagtgctg
      541 aagcccatca aatggatcag tgtccggcgc aatgaagtcg gggccgtagt ttcgaccagg
      601 aacgttcagg ctgctatgag cgcaggctcc ggcgagttag gactccacgt tgaggatgag
      661 cgccaacagc gtgcgggact gtttctccgt gatgtgacct accgcatcca tgcctacttt
      721 gaactccgtg accccagttt tcataagtct cattctaccc aactttccaa agttccggtc
      781 aatcacgagg aagacgatct gctggcactg tccgataaca atgctacgaa attcatgagt
      841 atgttcgaac gccgggcctc acgtggccaa tgcgtcaacc aaccgtacct cggctgccgc
      901 gagttcgcct gttcgttccg attcatagag gacgtgaaag ccgaaccaaa accgatcgaa
      961 gaaacaaagg acctaggctg gatgctctac gacttggatt acagtaatcc tgccgatcct
     1021 aagccgcgtt tcttcaaggc tcgattagac aacggcgttg tcatggtgcc tgcctgggaa
     1081 agcgaggagg tgcggggatg atcctccaat cactgcatga ctattaccaa cgcaagccag
     1141 atttgcctcg cgaagggttc gaggaaaaag aaataccttt catcatcgag atcgatagga
     1201 acggtgtagc ggctcagatt gaagatacgc gtacccagga tggaaaaagg aaaagagcga
     1261 agaagtcagt caatgtggca gccaatctcc tttggggtaa tgccgaatat gtcttgggac
     1321 tgccggactc gaaaaagctt ggagagcaaa agggcaaggg caaagaactt gagtatcaag
     1381 cgcgcctaaa ggagatgcgg ggggcattta ttaaggagat tgcgtctctt ccaccaccat
     1441 gtcagaccga tccaggcatt caggctgtcc tgaaattctg tgagcagtcc gactttactg
     1501 atcttgcgcg attcggtgaa acatggaaag acgtgcaagc aagtaaccag agcactacct
     1561 tccgcctcca gggtgacaca ggacttgtct gccaacgaca ggccgtaatt gaggcggtgt
     1621 cgaaatcagt taccaagg
//
LOCUS       DAORQN010000299         1625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1849_length_1625_cov_4.936306, whole genome shotgun sequence.
ACCESSION   DAORQN010000299 DAORQN010000000
VERSION     DAORQN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_1625_cov_4.936306"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1063)
                     /gene="ttcA"
                     /locus_tag="PK614_10010"
     CDS             complement(188..1063)
                     /gene="ttcA"
                     /locus_tag="PK614_10010"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRB82578.1"
                     /translation="MQSTTTPSSPGTAQPTRHSNEIERVHTRLCRLVGQAIADYCMIE
                     EGDKVMVCLSGGKDSYGLLEILLSIRSRAPIHFDIIAVNLDQKQPGFPAHVLPEYLTR
                     RGVPFHIETRDTYSVVKRLIPEGQTTCSLCSRLRRGHLYRLATELGATKIALGHHRND
                     ILETLLLNLLFTGKMKAMPPKLRSKSGRHVVIRPLAYVKEADLVRYAALLQFPIIPCD
                     LCGSQEDLKRKQVKTLLQDWDQRFPGSNDSMFAALGNIAPSLLLDRTLFDFSSLKADA
                     SPTEPAASDSEDDLL"
     gene            1142..1609
                     /locus_tag="PK614_10015"
     CDS             1142..1609
                     /locus_tag="PK614_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRB82579.1"
                     /translation="MTSILFVCSGNIFRSLVAEYALKAQLGSRVGYLIGSAGIEALPQ
                     EIHPVVLAGLREKGVDPSRHRQRKLTRDLIEETAVVIAMGRNHQAWIRGEFGREVPLF
                     NHVCYGIDQPILDVHEIIPDWREQPDQSRDYLQFVIDHIWGGVPHLLNRVPAL"
BASE COUNT          313 a          438 c          526 g          348 t
ORIGIN      
        1 ctgtctagca ggatgcggaa aaagtccgcc aacggcgttc tcgcatcgct tagaggctca
       61 acgctggacg gccattttgc gcatcctgcg gggtattctt ctgctaggcc actcgatgtg
      121 gaccatctga gtgttcgcat gccaagcggg ttttcccaca gcctgctaga ggcagcagcg
      181 cgtcgggcta gagcaaatcg tcttcagaat cactcgcggc tggttctgtt ggggaggcat
      241 ctgccttgag ggatgagaag tcgaataggg ttcgatccaa cagcagcgac ggtgcgatat
      301 tgccgagggc ggcgaacatg ctgtcgttgg aaccggggaa ccgttgatcc cagtcctgca
      361 gtaaggtctt gacctgcttc cgcttgaggt cctcctgtga gccacagagg tcgcagggga
      421 tgatcggaaa ctggagcaat gcggcgtacc gcaccagatc ggcttccttc acataggcca
      481 gggggcgaat aactacatgg cggccgcttt tcgaacgcag cttgggcggc atggccttca
      541 tcttgccggt gaacaacaga ttcagcagca gcgtttccag aatgtcattg cggtgatggc
      601 caagggcaat cttggtggcg cccagttccg tggccaaccg gtagagatgg ccacgccgga
      661 ggcgcgagca gagtgagcag gtagtctggc cttcggggat gagacgtttg acgacggagt
      721 aggtatcgcg cgtttcgata tggaagggca cgccgcgcct ggtcaggtac tccggaagaa
      781 catgggcggg gaaccccggt tgcttctgat ccaggttgac ggcaatgatg tcgaaatgaa
      841 tcggcgcacg gctgcggatc gacagcagga tctccaggag cccgtaactg tctttcccgc
      901 cggaaaggca gaccatgacc ttgtcgcctt cctcgatcat gcagtagtcg gcaatggctt
      961 gtcccaccag gcggcagagt cgggtgtgca cgcgttcgat ctcgttggag tgcctggtcg
     1021 gctgtgcggt gcctggggat gatggggtgg ttgtgctctg catggcccgg cattgtagcg
     1081 caaccggggc cgtgattggg atagccgtga acaggggcat tcagactgac aggctgaggc
     1141 gatgacgtcg attctgttcg tgtgctcggg caatattttc agaagcctcg tggccgaata
     1201 tgcgttgaag gctcagctcg gctcgcgggt cgggtacctg atcggatccg ccggcatcga
     1261 ggcgttaccg caggagatcc atcccgtggt cctggccggc cttcgtgaga agggggtcga
     1321 tccgtcccgc caccgtcaac ggaagctgac gcgcgacttg atcgaggaga ccgccgtcgt
     1381 cattgccatg ggcaggaacc atcaggcgtg gattcggggg gaattcggtc gagaagtccc
     1441 tctcttcaat cacgtatgtt acggaatcga tcagccgatt ctggacgttc atgagatcat
     1501 ccccgactgg cgggaacaac cggatcaatc gcgtgactat ctgcagtttg tcatcgacca
     1561 catctggggc ggagttcccc atctgctcaa tcgtgtgccc gccctgtaat ccgtaaccct
     1621 tcttc
//
LOCUS       DAORQN010000300         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1860_length_1612_cov_5.658317, whole genome shotgun sequence.
ACCESSION   DAORQN010000300 DAORQN010000000
VERSION     DAORQN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1860_length_1612_cov_5.658317"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..706
                     /locus_tag="PK614_10020"
     CDS             86..706
                     /locus_tag="PK614_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRB82580.1"
                     /translation="MSVRSILIHVTEVPMLGTLVRLYRGIQELRNQVIEDWTVNSRDW
                     TMPGFRAIALHRLGTWLANRRSGVVKSGLLAVYRVLYRYVRNHYGIEIPLTVTIGRRL
                     WLGHQHGIVFHWKAEIGDDCLIRHGATIGAGYGGQKTLHKGPKLGQRVEVGPGAVIFA
                     GVKIGDDAVIGPNAVVMSDVPAGARVFAEAPRVIHLPTAHQPARKN"
     gene            831..>1612
                     /locus_tag="PK614_10025"
     CDS             831..>1612
                     /locus_tag="PK614_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methoxymalonyl-ACP biosynthesis protein FkbH"
                     /protein_id="HRB82581.1"
                     /translation="MNIARAQCAASSITPETRGDAFRKKGQVEQAIEAYLEGVAAPPS
                     AALCLKLARCYEQMANYAEACRWALSIVDAGDDFTSWQAGWALFQRNAQKARRPAVRS
                     IKVALLGSYTTTQLCPMLCLAAGKLGIHIDLYESRYGQYQQDILDHKSGLYAFNPNIV
                     VLAVHEGDLHLPEYSQRPEEDVRKEVSRWTGLWQMVTEHSRARIVQHNFALPCEIPTG
                     HLTTRLSGSRYMMTQAVNTRLGEAAANAVSLVDCERLSALIG"
BASE COUNT          357 a          475 c          463 g          317 t
ORIGIN      
        1 cggttggagc cccagccact tcgccggttg ctcagatctc gttggactcc tcacctgcga
       61 agactcagcg acaagacagc accacatgag cgttcgatcc atactcatac acgtgaccga
      121 ggtgccgatg ctggggacac tcgtcaggct ctatcgaggc atacaggaac tacgaaacca
      181 ggtcattgaa gactggaccg tgaacagccg cgactggacg atgcctggct ttcgagcgat
      241 cgccttgcat cgcctcggca cctggctcgc gaaccggcgc agcggcgttg tcaagtccgg
      301 actgctcgcc gtgtaccgtg tgctgtatcg ctatgtgcgc aatcattacg ggattgaaat
      361 tccattgacg gtcacaatcg gccggagact ctggcttggc caccaacatg ggatcgtctt
      421 tcattggaaa gcagagattg gagatgactg cctgatccgc cacggcgcca caatcggagc
      481 gggatatggc gggcaaaaga cgctacacaa gggcccgaaa cttggccaac gagtggaagt
      541 gggccccggc gcggtgatct ttgccggagt caaaatcggc gacgatgcgg tcatcggccc
      601 gaatgcggtg gtgatgtcgg atgttccggc cggcgcaaga gtgtttgccg aggcgcctcg
      661 ggtcatacat ttgccgaccg ctcatcagcc agctcgcaag aactaacgac ctacggccgg
      721 agaatgaata cgaaggaacc tcgcgctatt caccgtccag ggatcgactc aaccacagac
      781 gcctcttatc cggtgagtgt actgctcata gaaatgacag gccgtccctc atgaatattg
      841 cccgagcaca atgcgccgct tcatctatca cgcctgagac acgtggcgac gccttccgta
      901 agaagggtca ggtcgaacag gcgatcgagg cctatctgga gggcgtggcg gctcccccat
      961 cggctgcgct gtgcctcaaa cttgcgcgat gttacgagca gatggcgaac tatgcggagg
     1021 cctgtcgatg ggctctttcc atcgtcgatg caggcgatga ctttacgtcc tggcaagcag
     1081 gctgggcatt gtttcaacgc aatgcacaga aagcccggcg accggcggtc aggtcgatca
     1141 aagtcgcgct cctcgggagc tataccacaa cccaactgtg cccgatgttg tgccttgcgg
     1201 cggggaaact cggcatccac atcgacctgt atgaaagccg atacgggcaa taccagcagg
     1261 acattctcga ccacaagagc ggactctatg cgtttaaccc gaatatcgtc gtccttgcgg
     1321 ttcacgaggg tgacctccat cttcctgaat acagccaacg ccctgaagaa gacgttcgca
     1381 aagaagtgag tcgctggacc ggcctctggc agatggtgac tgaacactcc cgcgcccgaa
     1441 tcgttcaaca taactttgca ttgccctgcg agatccccac aggccatctg accaccaggc
     1501 tttccggctc tcgatacatg atgacgcagg ccgtcaatac gaggctgggt gaagcggctg
     1561 cgaacgccgt ctccctggta gattgcgagc gtctttcggc gctcattgga aa
//
LOCUS       DAORQN010000301         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1864_length_1606_cov_6.987750, whole genome shotgun sequence.
ACCESSION   DAORQN010000301 DAORQN010000000
VERSION     DAORQN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_1606_cov_6.987750"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..279)
                     /locus_tag="PK614_10030"
     CDS             complement(76..279)
                     /locus_tag="PK614_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82582.1"
                     /translation="MAKKPVGESDEVRLKKKIAVRVTKEAKLSGDPAARSLHKRLKRV
                     QRKRRALALRKKHAAGHQTEAKS"
     gene            complement(407..1066)
                     /locus_tag="PK614_10035"
     CDS             complement(407..1066)
                     /locus_tag="PK614_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRB82583.1"
                     /translation="MDMNSRNPFRRKAGYAVIPLAGCLLLSACSALPKGELTLDLGIK
                     DRGVASWYGKEFHGKLAANGEAFDMTAYTAAHRKLPLGSVVRVVNLTNGKTVQVRIND
                     RGPYVAGRMLDLSHAAARELGMVEAGTTAVQIEVIGDHRPVAQIPSTKIPAVAGMVLN
                     TDMRAVRQHRRPEEGVERPAVPVRMMPQEALYVRRERRIGSMLAADHTAHNTVPVLIL
                     S"
     gene            complement(1418..>1606)
                     /locus_tag="PK614_10040"
     CDS             complement(1418..>1606)
                     /locus_tag="PK614_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82584.1"
                     /translation="IQASNREDNSSLVRTDAHSGSHDNVLWFEPQDGESILSEVLEKN
                     FERFVVNTKFEDRDIRFR"
BASE COUNT          396 a          463 c          372 g          375 t
ORIGIN      
        1 gtgtgttcca tatcaatgtt ccttgttccg cagaggggtg ggcgtggcga tcaaatccgt
       61 caatcccgca accgcttagg acttcgcttc agtctgatgt ccagcagcat gcttttttct
      121 caacgcaagc gcacgccgct tgcgttgcac tcgcttgagc cgtttgtgta aagagcgggc
      181 agccggatca cctgaaagtt ttgcttcttt tgttactcgt accgcaattt ttttcttgag
      241 tcggacctcg tctgattcac caactggttt ctttgccatt cagctttcaa cctccaacaa
      301 ttaagatcaa caaacataag gaatgacggc gcaatcaggc atcactccag aggaggcagt
      361 ctaatcaagt acgggcggaa gtgtcaacta acggagtacc gcccacttag gataggatga
      421 ggacggggac ggtattgtga gctgtgtggt cggctgccaa catactgccg atacggcgct
      481 cacgcctcac atagagcgct tcttgtggca tcattcttac aggaacggcg ggtctttcaa
      541 ctccctcctc cgggcgacga tgttgcctca ctgctcgcat atccgtattc aataccatac
      601 cggccacagc aggaatcttc gtcgagggaa tctgcgcgac cggacgatga tctccaatca
      661 cctcaatctg caccgccgtg gtaccggctt caaccatgcc taactcgcga gccgccgcat
      721 gcgaaagatc cagcatacgc cctgcaacgt aaggcccccg atcgttgatt cgaacttgaa
      781 cggttttccc attggtcaaa ttgaccacac gaaccacact acctaacggc agtttgcgat
      841 gagcagcagt gtaggccgtc atatcgaagg cctccccatt cgctgcgagt tttccatgaa
      901 actccttgcc gtaccatgag gccactcccc gatctttaat gccgaggtcc aatgtaagtt
      961 cgcctttcgg caacgcggaa caagcactca agagaagaca gccagccaga ggaatcacag
     1021 cgtaaccggc cttacgacgg aaagggttcc gggagttcat gtccatagaa gcacctcgtg
     1081 tgaaagccta cacctcaccg cctgtcagac aactctccta ctcagtgagg tcaggctacg
     1141 caagattgcc cttggcgaca ataccgtctg ggtagcaacg ccccctacaa atgggcagac
     1201 aattcaacgc tcaacgaaga agaagtctat gattttgctg aacagagccg aacaacgaca
     1261 caaatgaatt tcttccgctc actgatacgc aggatgcctc aacgcttgga actgctgata
     1321 gttccagagt gaacacaagc ccaaattatc gggctctcgg atcaaccgta taaaatcagg
     1381 ccagcccgac agttcgattc cccactggca acacgatcta acgaaatcga atatcccggt
     1441 cttcaaattt ggtgtttact acgaaccgtt cgaaattctt ctccaacact tctgacagaa
     1501 tactttcccc atcctgcggc tcgaaccaca acacattgtc atggctacct gaatgagcgt
     1561 cggttcgtac gagtgagctg ttgtcttctc ggttgctcgc ctgaat
//
LOCUS       DAORQN010000302         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1874_length_1599_cov_3.957902, whole genome shotgun sequence.
ACCESSION   DAORQN010000302 DAORQN010000000
VERSION     DAORQN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_1599_cov_3.957902"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PK614_10045"
     CDS             <1..272
                     /locus_tag="PK614_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB82585.1"
                     /translation="QLTGQIYLGSEKFVAQHQPNRVIRDIPRRQTQAQRPSLETLFQR
                     KGDLEKTIHQAYRQYGYRLAEIADHLGVHAATVSRRLKRAEQENV"
     gene            307..651
                     /locus_tag="PK614_10050"
     CDS             307..651
                     /locus_tag="PK614_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRB82586.1"
                     /translation="MLNDKVLAAVLEFRRKRDWEQFHRPKELAAAITVEASELLEIFQ
                     WKSHDEVARLLESPSRERVEDEIADVVILLSYLCHDLGLDVNAAVLSKLKKNEAKYPV
                     EKSYGNARKYDE"
     gene            684..1403
                     /locus_tag="PK614_10055"
     CDS             684..1403
                     /locus_tag="PK614_10055"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HRB82587.1"
                     /translation="MMLRSVLLFVAVSVAFAGCGATRHARSVEPSGFLQDLYAQMREG
                     KGDEALRVYRNPAVDWPAKAAYSKILLDPVMIWRGKDSKVDGLNPKEAQVIADSFYAL
                     LYQELAKDYEMVSEPGSKTFRMQAALTDAEQSYPALDIVSSVPAPFNIAFVASSLKTL
                     TTGKPLFKGAASIEGKLMDAESGEILAAAVDRRVGGRFLDAEVFDSWNDVHGALKYWS
                     QLTRFRLCQLRKQANCVSPGS"
BASE COUNT          330 a          439 c          497 g          333 t
ORIGIN      
        1 agcaactcac cggccagatc tacctggggt cggaaaaatt cgtggcgcag caccaaccga
       61 atcgggtgat ccgcgacatt ccgcgccgac agacgcaagc ccagcgcccc tcattggaaa
      121 cgctgtttca gcggaagggc gacctggaga agacgatcca tcaggcgtat cggcagtacg
      181 ggtatcgatt ggccgaaatt gcggaccatc tgggtgtgca tgccgccacg gtgagccgcc
      241 gcttgaagcg ggccgagcag gaaaatgtgt gattgcaaga cctgacccca aagagagagg
      301 acctcgatgt tgaacgacaa ggtgctggcg gcggtgttgg agtttcgacg gaagcgcgat
      361 tgggagcagt ttcaccggcc caaggaactt gcggccgcca ttacggttga ggcgagcgaa
      421 ttgctggaga tcttccaatg gaaatcgcac gacgaggtgg cccgtctcct ggaaagcccg
      481 tcacgggaac gcgtggagga tgaaatcgcc gatgttgtga tcctgctttc ctacctgtgt
      541 catgatctgg gcctggatgt gaatgccgcg gtgctctcga agctgaagaa gaatgaggcc
      601 aaatatccag tcgagaagtc ctatggcaat gcccggaagt atgacgaatg atcgagcctc
      661 tttacctgtt ccagggaggt atcatgatgt tgcgatctgt cttgctattc gtggcggtct
      721 cggtcgcgtt cgccggctgc ggggcgacca ggcatgccag gtcggtggag ccgtccggat
      781 ttctgcagga tctctatgcg cagatgcgcg agggcaaggg cgacgaagcg ctccgggtgt
      841 atcgcaaccc ggcagtcgat tggccggcga aggccgcata cagcaagatt cttttagatc
      901 cggtcatgat ctggcggggg aaagactcca aggtcgatgg gctcaatccc aaggaggccc
      961 aggtgatcgc tgattccttc tacgcgctcc tgtatcagga actcgccaag gactatgaga
     1021 tggtgagcga accgggctcc aagacgtttc ggatgcaggc cgcgctcacc gatgccgaac
     1081 aatcttatcc agccttggat atcgtctcga gtgtgccggc gccgtttaac atcgccttcg
     1141 tcgcctcgag tctcaagacg ctcaccaccg gaaaaccgtt gttcaaaggc gcggcctcca
     1201 tcgagggcaa actcatggat gcggagtctg gggagatcct cgcagccgcc gtggatcggc
     1261 gggtcggcgg gcgctttctc gacgcggagg tcttcgattc ctggaacgac gtgcacggcg
     1321 ccttgaagta ctggtcgcag ttgacccggt ttcgcttgtg tcagttgcgc aagcaagcca
     1381 actgtgtctc gcccggctcg taggtcgatc ttttcagctg ccttcaaaca cctcgctcag
     1441 ttgaagcttg ttagccgacc aggtaccatg cctgcctcat cttgtgtgga ggcactatgg
     1501 cgcagattac cgaagttgct ccagacctgt ttcgcatcac caccttgaac tggcccgcga
     1561 cattgtgact gatcggacct gctggggaag ctagtgact
//
LOCUS       DAORQN010000303         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1891_length_1589_cov_11.211213, whole genome shotgun sequence.
ACCESSION   DAORQN010000303 DAORQN010000000
VERSION     DAORQN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1891_length_1589_cov_11.211213"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PK614_10060"
     CDS             <1..539
                     /locus_tag="PK614_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82588.1"
                     /translation="ARIYAEVIGYAMNSDAYHITAPPEEGEGAVRCMEMALKDAGVAK
                     TDIGYINAHGTSTMADAIETKAIKHVFGEQAYRIPVSSTKSMTGHLLGAAGGIEAVFS
                     ILALHHGILPPTINLDHPDPACDLDYVPNSAFRPDAGGPFKLLRLWRGERLPALSSVC
                     SVIHFRARVRSYDAGNVN"
     gene            517..1221
                     /gene="rnc"
                     /locus_tag="PK614_10065"
     CDS             517..1221
                     /gene="rnc"
                     /locus_tag="PK614_10065"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRB82589.1"
                     /translation="MTPATSIEAVQRLLGYRFHQPRLLEEALTHKSYSNERRSKDRTQ
                     NERLEFLGDAVLSLVMSEYLAAEFPGSNEGGLSKLKAHLVSEASLAKAARRMKLGRLL
                     RLGKGEELSKGREKHSLLADALEALIAAIYLDGGLEASRKFTLQVLEEELLATRAEQA
                     RPGMEDYKTQLQEVCQKRYESLPQYETVRESGPDHEKVFEVELTIQGVMRGIGRGRSK
                     KEAEQMAAKEALTQLA"
     gene            1259..>1589
                     /locus_tag="PK614_10070"
     CDS             1259..>1589
                     /locus_tag="PK614_10070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB82590.1"
                     /translation="MAQQRWSNRLRIAGMMSLFMTVGIAGIGLAADAAPAGSESVSAS
                     NARAVIKTKFGDIEIKFLPDVAPKHVENFIKLAKSGFYNGTIFHRAIPGFMIQGGDPN
                     TKDSLKKG"
BASE COUNT          348 a          412 c          515 g          314 t
ORIGIN      
        1 gcgcccgcat ctatgccgaa gtgatcgggt acgccatgaa cagcgacgcg taccacatta
       61 ccgccccgcc tgaagagggg gagggggcgg tccgttgtat ggaaatggca ttgaaggatg
      121 cgggtgtggc caaaacggac atcggttata tcaacgccca cggcacatct accatggcgg
      181 atgccatcga gaccaaggcc atcaaacatg tgtttgggga gcaagcctac cggattcctg
      241 tcagttcgac gaaatccatg acgggacacc tcttgggtgc ggccggcggt attgaggcgg
      301 tgtttagcat tttggcgctg caccatggca ttttgcctcc cacgatcaac cttgatcatc
      361 ccgatcccgc ctgcgatctt gactacgtgc ccaatagcgc gttccgtcca gacgcaggtg
      421 gtcctttcaa actccttcgg ctttggcggg gtgaacgcct gcctgctctt tcgtcggtgt
      481 gctcagtaat ccacttcagg gcgcgagtca ggtcgtatga cgccggcaac gtcaattgag
      541 gcggttcagc gccttctcgg ctaccggttc catcagcccc gtctgttgga agaagctctg
      601 actcacaagt cctactcaaa cgaacggcgc agcaaagatc gaacccagaa cgaacgcctg
      661 gagtttctcg gcgacgcggt gctctcgctt gtcatgagcg agtatctcgc ggccgagttt
      721 ccaggtagca acgagggcgg gctttccaag ctcaaagcgc atctggtcag cgaggcttcg
      781 ctcgcgaagg cggccaggcg catgaagctg ggtcggctgt tgcgtctggg gaaaggcgaa
      841 gagctctcga aagggcgcga gaagcattcg ctgttggccg acgcgctcga agcgctgatt
      901 gccgccatct acctggacgg tgggcttgag gccagcagaa aattcacctt gcaggtgctg
      961 gaggaggaat tgctggcgac gcgcgctgag caggcccggc cgggaatgga ggattacaaa
     1021 acgcagctgc aggaggtctg tcagaaacgg tatgaaagtt tgccgcagta tgagacggtt
     1081 cgggaatcgg gtcctgatca tgagaaagtt tttgaggtcg agctgaccat tcagggagtc
     1141 atgcgaggga tcgggcgcgg tcgcagtaag aaagaagcag agcaaatggc ggcgaaggaa
     1201 gcgttgacgc agttggcgtg agcaagaggt cgcggaccga ctagaaggag gcttggtgat
     1261 ggcgcagcag cgatggagca atcgtttaag aatcgcggga atgatgagtc tgttcatgac
     1321 cgtcgggatc gcgggaatcg gcctggccgc cgatgccgct ccggccggaa gcgaatctgt
     1381 cagtgcgagt aacgcgcgtg cggtcatcaa gacgaagttc ggcgatatcg agatcaagtt
     1441 tcttccggac gtcgccccga agcatgtcga gaatttcatc aagttggcca agtcggggtt
     1501 ctataacggt acgattttcc accgggcgat cccgggattc atgatccagg gaggcgatcc
     1561 caacacaaag gattcgctga agaaggggg
//
LOCUS       DAORQN010000304         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1899_length_1585_cov_5.656209, whole genome shotgun sequence.
ACCESSION   DAORQN010000304 DAORQN010000000
VERSION     DAORQN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1899_length_1585_cov_5.656209"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>1585)
                     /locus_tag="PK614_10075"
     CDS             complement(62..>1585)
                     /locus_tag="PK614_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB82591.1"
                     /translation="TERIFLAPTFGWEMSSRTTLRFEAEYMYDRAPIDRGLVAIGNGV
                     APIPVSRFLGDPSRKLETHHGKATITLWHDISNMFRWRTAFRTAVTSSRYSSLESNFL
                     VGAESDGILNLARYEIPTTVQSHYLQNELHGNFTTGSIKHKTIIGIELGRENSSATAS
                     GDFGGDTSTPGAFSYINIFNTNDRLFLNPALTKFSDASTQNNILGAYFGDQVDLLDNL
                     HVHFGGRFDLFDQTITNHPDDFTATSSQNNKTDSAFSPSVGVAYQPWKPITLFANYTE
                     SFAPQSASSRSINGNLFNPERGKSYEGGIKYQAFGGRLRSTVALFDTRKKNVLTADPL
                     NGFFFSVATGEQRSKGVEFDIAGQILPGWDIIANYAYIDARVTKDLLFAEGSRAPNSA
                     LHQGSLWTTYFFQEGVVQGFGAGIGMYAQGKRNGIFQCQDPANCQAPFEMAGYVRMDA
                     ALYYRKQEVFNKTNLLAAINFTNVLDHRYFSGAQNFREIVYTGAPFTAIGSLKFEFH"
BASE COUNT          322 a          412 c          481 g          370 t
ORIGIN      
        1 gaggggagtc tcgatgtgag actcccctgt tgcggttgcg gcagtcggaa gggcgacgag
       61 gtcagtgaaa ctcgaacttg agggatccga tggctgtaaa cggcgcgcca gtgtacacga
      121 tctcgcggaa gttttgcgca ccgctgaaat agcgatggtc gagaacgttg gtgaaattga
      181 tggctgccaa caggttggtc ttgttgaaga cttcctgctt ccgatagtag agcgcggcgt
      241 ccatgcggac atacccggcc atctcgaatg gtgcttggca attggcggga tcctgacact
      301 ggaaaattcc gtttcgcttt ccctgtgcgt acatcccgat accggcgccg aaaccctgca
      361 cgacaccttc ctggaaaaaa taggtcgtcc acaaacttcc ctggtgcaag gcgctgttcg
      421 gtgcccgact tccctcggcg aacaggagat ccttggtcac acgcgcgtcg atgtaggcat
      481 agtttgcaat gatgtcccat ccgggcagga tctggcccgc gatgtcgaac tccacgcctt
      541 tgctgcgttg ttctcccgtc gcaacggaga agaaaaagcc gttgagcgga tcggccgtga
      601 gcacattctt cttcctggta tcgaagagcg cgaccgtcga acgtaaccgt cctccgaagg
      661 cctgatattt gatgccgccc tcataggact tgccgcgttc cggattgaac aggttgccgt
      721 tgatgctcct ggaacttgcg ctttgcggcg cgaaagactc cgtatagttg gcaaagaggg
      781 taatcggctt ccagggttga taggccacac cgactgaggg actgaaggcc gaatcggttt
      841 tgttgttttg gctgctggtg gcggtaaaat cgtcgggatg attcgtgatg gtttgatcga
      901 agaggtcgaa ccgtccgccg aagtgcacat gcaggttatc caacaggtcg acttggtcgc
      961 cgaagtacgc acccaggatg ttgttctgtg tgctggcgtc actgaatttc gtgagggccg
     1021 gattcaagaa taaccggtcg ttggtattga atatattgat atagctgaac gcgcccggcg
     1081 tgctcgtgtc gccgccgaaa tcccccgagg ccgtggccga tgagttttct cggccgagtt
     1141 cgattccgat gatcgtttta tgtttgatcg agccggtcgt gaagttgcca tggagttcat
     1201 tctgcaagta gtggctctgg accgtcgtcg gaatctcata ccttgccaga ttcaagatgc
     1261 cgtcgctctc ggctccgacc aggaagttgg attcaaggct ggaatagcgg ctgctggtga
     1321 cggcggttct gaacgcggtt cgccaccgga acatattgct gatgtcatgc cacagagtaa
     1381 tggtcgcctt cccatgatgg gtctccagct tgcgggaggg atcacccaag aagcgcgaga
     1441 ccggtatcgg agcgaccccg ttgccgatgg cgacgagccc tcgatcgata ggcgcgcggt
     1501 catacatgta ttcggcttcg aaccgcaacg tcgtccgtga actcatttcc caaccgaagg
     1561 tcggggcgag gaagatacgt tcggt
//
LOCUS       DAORQN010000305         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1902_length_1584_cov_7.995422, whole genome shotgun sequence.
ACCESSION   DAORQN010000305 DAORQN010000000
VERSION     DAORQN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_1584_cov_7.995422"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK614_10080"
     CDS             <1..970
                     /locus_tag="PK614_10080"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRB82592.1"
                     /translation="ALGVTRLITIGGDDTAFSALKLEQRAAGRLQVVHVPKTIDNDLD
                     LPHGIPTFGFQTARHIGVELVKSLMVDAETTSRWYFVVTMGRKAGHLALGIGKAAGAT
                     LTVIPEEFRQKPLRLKTLVDVLAGAFIKRLNAGRSDGVAVLAEGLVEILDQQDLDGLQ
                     DVERDQHGHVRLAEINFGFVLKRAVEKELRSFGIKTTIVEKNIGYELRCADPISFDME
                     YTRDLGYCAAQFLLEGGNAAMVSIQNGRFIPIPFGDILDPATGRTRVRMVDVESESYV
                     IARRYMIRLAPDDFNQPQALVRLATTAGLAADDFRKRFEYLMGKDL"
     gene            1022..>1584
                     /locus_tag="PK614_10085"
     CDS             1022..>1584
                     /locus_tag="PK614_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82593.1"
                     /translation="MTSKATPDTEIALAFGKQRALVSPWGAALRRYYLLEGNGSETDI
                     VWGYAGGVNKKGGQGDVLIPFPGRVAEGRYSFDGRQLQLERNDKEGPNAIHGFVRTLP
                     WTTQQADSNSVAFEVQLEAANYASRGYPFSLAVRVTYGLDAKGLTCSFAVHNVGREVA
                     PVGVGFHPYFTSAPEASRPLATFSPWRG"
BASE COUNT          299 a          420 c          527 g          338 t
ORIGIN      
        1 cgcactcggc gtgactcgat tgattacgat cggaggggac gataccgcgt tctccgcgct
       61 gaagttggag cagcgggccg ccggtcgcct ccaggtcgtg catgtgccga agaccatcga
      121 taacgatctg gacctgcccc acgggattcc gacattcgga ttccagacgg cgcgccacat
      181 cggcgtggag ctcgtgaaga gtttgatggt ggatgcggag acgacctcac gctggtattt
      241 tgtggtgacg atggggcgta aggccgggca tctggcactc gggatcggga aggcggccgg
      301 tgcgacgttg acggtgattc ctgaggagtt tcgacagaag cctcttcgtc tgaaaacgct
      361 ggtcgatgtc ctggccggcg cgttcatcaa gcggctcaat gccggacgat cggacggcgt
      421 ggcggtcctg gcggaaggtc tcgtggaaat cctcgatcag caggatctcg acggtctgca
      481 ggatgtggag cgggaccagc acggacatgt gcggctggcg gagatcaact tcggcttcgt
      541 gctcaagcgg gcggtcgaga aggaattgcg ttccttcggc attaagacca ccatcgtcga
      601 gaagaacatc gggtacgagc ttcgctgtgc cgatcccatt tcgttcgata tggaatatac
      661 ccgcgatctc ggttattgcg cggcccagtt tctcctggag ggcgggaatg ccgcgatggt
      721 gtcgattcaa aacggccggt tcattcccat tccgttcggc gacattctgg acccggcgac
      781 cggccgtacc agggtgcgta tggtggatgt ggagtctgag tcgtacgtca ttgcaaggcg
      841 gtacatgatt cgattggctc cggatgattt caatcagcct caggcgctgg tccgcttggc
      901 caccacggcc ggtctcgccg cggatgattt ccggaaacgg ttcgaatatc tgatgggcaa
      961 ggatctgtga gagaagggcg agggattgga catcgcccca tctgctgtga cgtagggcgt
     1021 catgacgagt aaagcaacac cggatacaga aattgccctc gcgtttggga agcagcgcgc
     1081 gctggtctca ccctggggtg cggcgctacg ccggtactac ctgcttgaag gcaacgggag
     1141 cgaaaccgac attgtctggg gctatgcagg cggcgtgaac aaaaaaggtg ggcagggcga
     1201 tgtgctcatt ccatttcccg ggcgtgtggc ggaggggcgt tactcctttg atggacggca
     1261 gctgcagctt gaacggaacg acaaagaagg tccgaacgcg atccatggat ttgtcaggac
     1321 cctgccgtgg accacgcagc aggccgactc gaatagtgtt gcatttgagg tccagctgga
     1381 agctgccaac tatgcctcca gaggctatcc tttctcgctc gcggttcgtg tcacctatgg
     1441 tttggacgcg aagggattga cctgctcgtt cgccgttcac aatgtggggc gtgaggttgc
     1501 gccggtcgga gtcggatttc atccctattt caccagcgcg ccggaggcga gcaggccgct
     1561 cgctactttt tctccttggc gcgg
//
LOCUS       DAORQN010000306         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1905_length_1582_cov_2.443353, whole genome shotgun sequence.
ACCESSION   DAORQN010000306 DAORQN010000000
VERSION     DAORQN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_1582_cov_2.443353"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..>1582)
                     /locus_tag="PK614_10090"
     CDS             complement(309..>1582)
                     /locus_tag="PK614_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HRB82594.1"
                     /translation="LSRIDVEGNQWFRSSYLRDRVAHGVSTPLSIQPLQERIQLLQQD
                     PRIERINAELKPGDVRGESVLNVRVKDANPFKAWLEFNNYQTPVVGAERGLATVAHQN
                     VTGHGDQFMFTYGRSHGVNPIIDTSYSVPLNSYETTLTAYYRRNAFLVVENPFRSLDL
                     NVDSEIIGISLRQPVYRTVTDEFALALTGEHLYLKTTSAFDAPGLPSLFIPGSSTTGV
                     ATVSALRFSQEYTHRSPSFVFAALSRVSIGLNVLGATTNSGPLPDGQFVSWLGQAQAV
                     KRVEDWWGVQFLGRVAAQVASDRLFPLEQMPVGGRFSVRGYRENTLVRDDAVLASFES
                     RFPLLRFASGEDRLQFAQFVDFGHAWNAKGNTPDPQTLASVGAGLRWMILPQERARFE
                     VYWGQQLNHVRGGEGNLQDHGIHLQLVVQVL"
BASE COUNT          351 a          453 c          456 g          322 t
ORIGIN      
        1 ctgaaaacct ctgtcgcaac ggtgtgactt caatcgttca catgggaggc acccgtagga
       61 tgctcaaaaa gaccgtcctc ttcgttcgtc attcgtgaag cgtcgttcgt ctccggttca
      121 aagaaaagag cctatggcat atagccaata gcgtatggca aagaatagag cttgaggggc
      181 gttgtgacca gccataagcc tcgtgccatg tgctcttatc tccggccatc agctctggtc
      241 tccaatgaga tacgcttcac gattcacggc tgttgagcga gaagctggcg gactttttca
      301 gcatcccatt agagtacctg cacgacaagt tgaaggtgga tgccgtgatc ctgcaggttg
      361 ccctcgccgc cccggacatg attgagttgc tgaccccagt acacttcgaa gcgggcccgt
      421 tcctggggca agatcatcca acgcaacccg gccccgacgc tggccaaggt ttgaggatcg
      481 ggggtgttgc ctttggcgtt ccaagcatgt ccgaaatcga cgaattgcgc aaactgcaac
      541 cggtcttcac cggatgcgaa ccgcagcaag ggaaatcgcg attcaaacga agccagcacg
      601 gcatcgtccc ggactagtgt attttctcga tacccgcgca cgctgaaacg gccaccgacg
      661 ggcatttgtt cgaggggaaa gagccgatcg ctcgccacct gcgccgccac gcgtcccaag
      721 aactggacgc cccaccaatc ctccactcgt ttcaccgcct gcgcctgacc cagccaggaa
      781 acgaattgcc cgtcgggtag aggcccgctg ttggtggtgg cgcccaggac gttcaacccg
      841 atgctcaccc gggacagggc ggcaaagaca aaggacggag agcgatgcgt gtattcctgg
      901 ctgaatcgga gcgcactgac ggtggcgact cccgtcgtgg aagaacccgg gatgaacagt
      961 gatgggaggc ccggggcgtc aaacgccgac gtcgtcttca aatacaaatg ctcgccggtg
     1021 agagccagtg caaactcgtc ggtcacggta cggtacaccg gctggcgtag ggagatcccg
     1081 atgatttcgg aatccacgtt cagatccaag gagcgaaaag gattctcgac gacgagaaag
     1141 gcattgcgcc gataataggc tgtaagggtc gtctcatagc tattgagcgg cacactatag
     1201 gacgtatcga taatgggatt gacaccgtga gaccgtccat aggtaaacat aaattggtcg
     1261 ccgtgtcccg tcacgttctg gtgcgccacc gtcgccaagc cccgttctgc gccgacgacc
     1321 ggcgtttgat aattattgaa ctccagccag gctttaaagg ggttggcgtc tttcactcgc
     1381 acgttcagaa cgctctcgcc ccgcacgtct cccggtttca gctcggcatt gatgcgttcg
     1441 atgcgcggat cctgctgaag caattggatc cgttcttgaa gcggctggat gctgagcgga
     1501 gttgacacgc cgtgcgccac acgatcgcgt agatacgaac tgcggaacca ttggttgcct
     1561 tccacgtcga ttcgcgagag gg
//
LOCUS       DAORQN010000307         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1918_length_1568_cov_3.694646, whole genome shotgun sequence.
ACCESSION   DAORQN010000307 DAORQN010000000
VERSION     DAORQN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_1568_cov_3.694646"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK614_10095"
     CDS             <1..228
                     /locus_tag="PK614_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRB82595.1"
                     /translation="DYTPVCTTELGYMARIKGEFDKRGVKVLAISVDPLDSHRGWTKD
                     INETQNCTVSYQIIADQEKKLDDLYDMIHPN"
     gene            238..519
                     /locus_tag="PK614_10100"
     CDS             238..519
                     /locus_tag="PK614_10100"
                     /inference="COORDINATES: protein motif:HMM:NF021902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82596.1"
                     /translation="MTVRSVFVIGPDKKIKLMLTYPASCGRNFDELLRVVDSLQLTAK
                     YKVATPVNWKDGQDCIITPAVNDAEAKTLFPKGFKSIKPYLRLTPQPNK"
     gene            complement(881..>1568)
                     /locus_tag="PK614_10105"
     CDS             complement(881..>1568)
                     /locus_tag="PK614_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRB82597.1"
                     /translation="DLGTNNGVGGSFASGRREINWDGVPARFSDPNNLPGNFFNSNSA
                     RGAVFETPGSAFKVSANSGVGTPERFGSINPSFPNSFSVFSSQKLFTAIDSNIVDVRF
                     FVPGTNQPGAVRGFGSVFTDVDLAGPTTIEFFDFHNNSLGKQTVLNTPGVTSLSFLGV
                     SLEQAIINRVRITAGNAALSALDGFDFVAMDDFIYGEPQAVPAPAAVLLLGSGLAVLV
                     WARRKSLLPD"
BASE COUNT          375 a          415 c          470 g          308 t
ORIGIN      
        1 gactataccc ccgtctgtac gacagaattg ggttatatgg cgaggatcaa aggggagttc
       61 gataagcgtg gcgtgaaggt tctcgccatc agcgtggacc ccttggattc gcatcgcggc
      121 tggaccaagg acatcaacga aacccagaat tgtacggtca gttaccagat cattgcagac
      181 caggagaaaa agctcgacga tctgtatgac atgatccacc cgaactagct ggatagcatg
      241 accgtgcgat cagtattcgt gatcgggccg gataagaaga tcaagttgat gctgacctac
      301 ccggcttcct gcgggaggaa tttcgacgaa ttgctgcgcg tggtggattc cctgcagctg
      361 acggcgaaat acaaggtggc gaccccggtg aactggaagg acgggcagga ctgcatcatc
      421 acgccggcgg tgaacgatgc cgaagcgaag acgctgtttc cgaagggatt caaatcgatc
      481 aagccctatc tgcggctgac tccgcagccc aataagtagc gcgatctttg acggcggtcg
      541 agatgagagg agccggccac gaggtcggct cctcttttcg tgtcagcaag agccgttatc
      601 tgcaggacat tcatccaccg cctgagttgt tcccgctttg ccgcctggtt tcgtatccag
      661 tccaggtcaa tccacagcag atggatcggt ggccatcgcc ggcgtcctaa gggtgtcgac
      721 cgcgatccag aatagcgcgc ttgggtaacg tggcctgaag caagatacga ggtggcgaag
      781 caagagctcg ataggcttag gaaacgacgc gtagaacgca caagggggag tcagcttcag
      841 gccgactccc ccttgccatt ggaaaggtgc taaaccctgg ttaatcagga aggagagact
      901 tccgccgcgc ccacaccagg acagccaagc cgcttccgag gagaaggacg gcggccggag
      961 ccgggactgc ttgcggttcg ccgtagatga aatcatccat ggcgacaaag tcgaatccgt
     1021 ccagcgccga gagggcggcg tttcctgcgg taatcctgac gcggttaatg atggcctgct
     1081 ccaaagagac acccagaaag gaaagactgg tcacacccgg cgtgttgagg acagtttgtt
     1141 tgcccaggct gttgttgtga aagtcaaaaa actcaatcgt ggtaggaccg gccaaatcga
     1201 cgtcggtgaa gaccgagccg aacccgcgca cggctccggg ttgattggtg ccgggcacaa
     1261 agaatcttac atccacgatg ttactgtcga tggcggtaaa gagtttctgg gaactgaata
     1321 ccgagaaact attcgggaaa gatgggttga tgctgccgaa tcgctccggc gtacccactc
     1381 cagagtttgc actgaccttg aatgcggatc ccggcgtttc aaacacggcg ccgcgagctg
     1441 aattggaatt gaagaaattt cccgggagat tgttcgggtc agagaatctg gcaggcaccc
     1501 catcccagtt gatttcccgg cgaccgcttg cgaacgagcc acccacgccg ttgttcgttc
     1561 cgagatcg
//
LOCUS       DAORQN010000308         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1925_length_1562_cov_7.255474, whole genome shotgun sequence.
ACCESSION   DAORQN010000308 DAORQN010000000
VERSION     DAORQN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_1562_cov_7.255474"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK614_10110"
     CDS             <1..370
                     /locus_tag="PK614_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsI"
                     /protein_id="HRB82598.1"
                     /translation="MQRDFTYVDDIAEGVLRTLDRPAQADPAWASDNPDPGSSSAPYR
                     LYNIGNHQPVELLRFIEVLEQTLGMKAQKNFLPLQAGDVPATYADVADLMRDTGFKPA
                     TSIETGIARFVEWYRAYYKV"
     gene            528..1097
                     /locus_tag="PK614_10115"
     CDS             528..1097
                     /locus_tag="PK614_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82599.1"
                     /translation="MPGTFPVSRRQLSRLLFGFLVWLYSGMPRPVGTQMPIEPRIDGH
                     KPLILAPDSPDLWPGFRQQLTEWRDQARQELHYDDVRYRDESTSWAQSSLSSCFVMLC
                     DATFFDHELGQYRVREFLQQAQSDFGGFDNLILWHAYPRIGADDRNQFDFYRDMPGGL
                     QGIRDVVRRCHRFGVRAFVVYKPWDVGII"
     gene            1081..>1562
                     /locus_tag="PK614_10120"
     CDS             1081..>1562
                     /locus_tag="PK614_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82600.1"
                     /translation="MSVLSDVALLAELVELTEADGVYLDTMPVAPDGLRQALDGIRAG
                     LVIEGEGELPLASIQDHHMSWAQHFEDSHVPGVLRNKWFERRHMMHQTARWSRDHIPQ
                     LHTAWMNGAGMVIWENVFGSWVGWSARDRAILRTLLSIQRRFSSLFMGEGWMPLVPTE
                     L"
BASE COUNT          326 a          401 c          445 g          390 t
ORIGIN      
        1 gatgcagcgt gactttacct atgtcgacga tatcgccgaa ggcgtgttgc gcacgcttga
       61 tcggcccgct caagccgacc cggcctgggc gagtgacaac cccgaccccg gcagcagctc
      121 cgcaccctat cggctgtaca acatcggcaa tcaccagccg gtcgagctgt tgcgatttat
      181 cgaggtgctg gaacagacgt tgggcatgaa ggcccaaaag aacttcctcc ctctccaggc
      241 cggcgatgtg cctgcgacct atgccgatgt cgcggatctc atgcgtgata cgggcttcaa
      301 gcccgcgacc tctatcgaga ccggcatcgc ccgctttgtg gaatggtatc gggcgtatta
      361 caaggtgtga gaattctgaa gtgtttcgat cgtttggcct gagattgtcg cgagaagatt
      421 tggctaaagt gatggattgt aggcatcccg tctggtcgct cctccattga gagtgggctg
      481 ggcttagaga gaaactctca ttcattcctg cccatgatga gtcccgcatg cctggtactt
      541 tcccggtgag ccgtcgccaa ctttccagat tgctgtttgg tttcctggta tggctgtact
      601 ctggcatgcc tcggccggtg ggcacccaaa tgccaataga accgcgaata gatgggcaca
      661 agccattgat actagctccc gattcacctg acctctggcc aggatttcgc cagcaattga
      721 ctgaatggcg cgatcaggcc agacaagagt tgcattatga cgacgtccga taccgggatg
      781 aatcaacatc gtgggcacag tcgagcctgt ccagttgctt cgttatgttg tgtgacgcca
      841 ctttttttga tcatgagctg ggccaataca gggttagaga atttctccag caggcgcagt
      901 ctgactttgg tggtttcgac aatctgatcc tgtggcatgc ttaccctcgt attggagctg
      961 atgatcgaaa tcaattcgat ttctatcgtg acatgcctgg aggattgcaa ggcataaggg
     1021 atgtcgtgcg acggtgccat cgcttcgggg tgcgagcgtt cgttgtctat aaaccttggg
     1081 atgtcggtat tatctgatgt cgccttgctt gctgaattgg ttgaattgac tgaggcagac
     1141 ggtgtttatc tcgatacgat gccagtcgca ccggacgggc ttcgccaagc cttggacgga
     1201 attcgggcgg gactcgttat tgaaggagaa ggcgaacttc cgcttgcctc tatacaggat
     1261 caccacatgt cttgggctca acattttgaa gatagtcatg tgccaggagt attgaggaac
     1321 aagtggtttg agcgtcgcca catgatgcat caaaccgctc gatggagccg agaccacata
     1381 cctcagcttc atactgcttg gatgaacggg gccgggatgg tgatatggga aaacgtattc
     1441 ggttcatggg taggatggtc ggccagggac cgtgcaatcc tccgcacact gctgtccatc
     1501 cagcgtcgat tttcatccct ttttatggga gagggctgga tgcccctcgt ccccacggaa
     1561 ct
//
LOCUS       DAORQN010000309         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1926_length_1562_cov_5.583278, whole genome shotgun sequence.
ACCESSION   DAORQN010000309 DAORQN010000000
VERSION     DAORQN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1926_length_1562_cov_5.583278"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    315..324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(499..1431)
                     /locus_tag="PK614_10125"
     CDS             complement(499..1431)
                     /locus_tag="PK614_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB82601.1"
                     /translation="MMALFRRNRVWWMGFSYCGKQVRRSTEVTDKKVAEKIYHKVMTQ
                     IAEGKWYEPARGADKTVKELLERYLQDHSAPNKAPKSHRSDRSVSLHLIRTFGDLTLR
                     ELRPSLIAAHKAKRRAEGAAAKTINIELGILSHAFQLAVKEWEWVTENPVIRVSREKV
                     RNVIERWLTAEEEQRLLAASPQWLQEIIVFAANTGLRQGEILDLQWSRVDLFRRTITL
                     LEQKNGAKDTLPVNAKTLEVLKARARVRSLKNDYVFFNGTGNRMDARDLLRVFYPVMR
                     KAEVKNFRFHDLRHTFATRLVQAGVDIYTVQKLG"
BASE COUNT          337 a          460 c          373 g          382 t
ORIGIN      
        1 tggcagacaa ctttcacggg cgaaaagacc acctcctgcc cgcgaccctt cggccgatca
       61 ggctgagtga ccacaccgac cacctgatgt tccgacttaa gcaaggcctc cagagaaggc
      121 acggcaaagt caggagtccc cataaatacg atacgcatgc tcacgcttta tcattctgcc
      181 atcgcgaatg caattctccc cggacgttcc ttgactctcg tttcacacgc ctgttagtat
      241 ccggtcgcgg ggtggagcag cctggtagct cgttgggctc ataacacaaa ggtcgaaggt
      301 acaaataagc ccccnnnnnn nnnntctctt tcaaccactt acagcagtct cagtgccgac
      361 atcggcactc ctatgaggag ccgattgtgc taagtttgtg ctaactcctg ccggaacgcg
      421 atccaagatc tccacgccag cacgcaaact ctccggatga tgatgggcat accgcatcac
      481 catggagatg gttttccatc atccgagttt ctgcacggtg tagatatcga ccccggcctg
      541 cacaagacgg gtggcgaagg tatggcgcag atcgtgaaaa cgaaagttct tcacctctgc
      601 ctttcgcatg acaggataga acacgcgaag aagatctcgc gcatccatcc gattgcccgt
      661 accgttgaag aacacgtaat cgtttttgag cgagcggacc ctggctctgg ctttcagtac
      721 ctcgagggtc tttgcgttga ctggaagtgt atccttggct ccattcttct gctccagcaa
      781 cgtaatggtc ctcctgaaga gatcaaccct ggaccattgg aggtctagga tctctccctg
      841 tcgaaggcct gtatttgcag caaagacaat gatctcctgt agccattgcg gagaagcggc
      901 caacaatcgc tgttcctcct ccgccgtgag ccatcgctca atcacattac gcactttctc
      961 cctcgatacg cggatcaccg gattttccgt tacccactcc cattccttca cggcaagctg
     1021 aaaggcgtgg ctcaagatcc ctaactcgat attgatggtc ttggccgccg ccccttctgc
     1081 tcgtcgcttc gccttatgtg ctgcaataag agaagggcgc agttccctca gggtcagatc
     1141 cccgaatgtt cgaatgagat gcagcgatac agatctatca cttcgatggg acttgggggc
     1201 tttattcggc gctgaatgat cttggagata gcgctccaac aattccttca ccgtcttatc
     1261 ggctcccctc gctggctcat accacttgcc ttcagcgatc tgagtcatca ccttgtgata
     1321 aatcttttca gccactttct tatccgtcac ttcagtggat cttctgacct gcttgccaca
     1381 gtaagaaaac cccatccacc agactcggtt tcgccgaaac agtgccatca tcgacctcct
     1441 tgtttggccc tgtcttggtc tggcttctcg catctgggag tatagtgagc agtttttgcg
     1501 gcggcaatca ggtggtctac gtccctggcg tcatgccgag tgagtcgagg agggtgaacc
     1561 ct
//
LOCUS       DAORQN010000310         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1933_length_1556_cov_2.487009, whole genome shotgun sequence.
ACCESSION   DAORQN010000310 DAORQN010000000
VERSION     DAORQN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_1556_cov_2.487009"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PK614_10130"
     CDS             complement(<1..836)
                     /locus_tag="PK614_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRB82602.1"
                     /translation="MLAERRARYTQALCYDDLPGDVVHEVKRRILDSAACAFGAWKAT
                     PCRIARHVAQSAKVPGGATIWGTSHKTLPDLAAFANGALVRYLDFNDTYLSKEPAHPS
                     DNLAAILAAGEIAHASGKRVIQAIALAYEIQCRLCDAAALRSRGWDHVTYGSISSALG
                     AAKTLKLTEAQTTQAINLAAVANVALRQTRVGEMSMWKACAFSNAARNGLFAAILAQR
                     GMTGPAPIFEGEKGFMKLVSGPFELSRLGGERAPDDTLASFKILDSYIKHFPVEYHAQ
                     TAV"
     gene            complement(866..>1556)
                     /gene="meaB"
                     /locus_tag="PK614_10135"
     CDS             complement(866..>1556)
                     /gene="meaB"
                     /locus_tag="PK614_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRB82603.1"
                     /translation="AVARVITWIENQEPVGAAVLKRLTPPEKRAMVIGITGYPGTGKS
                     TLIDQLIAAYRRLGKTVGVLAVDISSPLTGGAVLGDRIRMQRHADDAGVYIRSMATRG
                     QSGGLARATRDALQVLEAAAYDVILIETVGVGQNDIEVVSVAGTVIALVAPGLGDDVQ
                     AMKAGLLEVADIVAVNKGDREGAATAVRDLRAWCPHVLRTLAVKGEGIPELIAMIAEQ
                     QRLQDLAHTPG"
BASE COUNT          268 a          494 c          495 g          299 t
ORIGIN      
        1 accgcggtct gggcatggta ttccaccggg aaatgtttga tgtaggaatc gagaatcttg
       61 aacgaagcca gggtgtcgtc gggagcccgc tctccaccca gtctcgacag ctcgaatggt
      121 ccggacacca gcttcatgaa gcccttttcg ccttcaaaga tgggggcggg gcctgtcatg
      181 cctcgttggg ccaggatggc cgcgaatagg ccgttgcgtg cggcattcga gaacgcacag
      241 gctttccaca tggacatttc accgactctg gtttgccgga gtgccacgtt cgccacggcg
      301 gccaggttga tggcctgcgt ggtctgcgcc tccgtcagtt tcagcgtttt ggctgcgccg
      361 agcgcggacg agatcgatcc ataggtcaca tggtcccacc cacgggagcg taacgcggcg
      421 gcgtcgcaga gacggcattg gatttcatag gccaaggcga tggcctggat gacccgcttg
      481 cccgaagcat gggctatttc gccggcggcg aggattgcgg caaggttgtc cgaaggatgg
      541 gccggctctt ttgagagata ggtatcgttg aagtcgaggt agcgaaccaa cgcgccgttg
      601 gcgaaggcag ccaggtccgg cagggtcttg tgggacgtcc cccagattgt cgcaccaccg
      661 ggaactttgg cggattgtgc cacgtgacgc gcgatccggc agggagtcgc cttccaggcg
      721 ccgaaggcac aggcggcgct gtcgagaatc cgtcgcttga cctcatggac cacgtcgccg
      781 ggcaggtcgt cgtaacaaag ggcctgggta tatctcgcac gacgctccgc aagcatgatg
      841 atcctctcga caacaggccg ttctgtcagc ccggtgtgtg tgcgagatcc tggagccgtt
      901 gctgttcggc gatcatggcg atcagctccg gaatgccctc gcccttgacg gccagggtgc
      961 gcagcacgtg cgggcaccac gcgcgtagat cgcgtactgc agtagcggcg ccttcacgat
     1021 cccctttgtt gacggccacg atatccgcca cctccagtaa ccctgccttc atggcttgca
     1081 cgtcgtcgcc caatcccggc gcgaccaggg cgatgacggt tccggccacc gagacgacct
     1141 cgatgtcgtt ctggccgacg ccgaccgttt cgatcaggat cacgtcatat gcggctgcct
     1201 ccaggacttg cagcgcatcg cgagtcgccc gcgcgagccc accggactga ccgcgggtcg
     1261 ccatgctgcg tatgtagacc cccgcatcgt ccgcgtgccg ctgcatcctg atccggtcac
     1321 ccagcaccgc cccgccggta agagggctgc tgatatccac ggccaggacg ccgaccgttt
     1381 tgccgaggcg gcggtaggcg gcgatgagtt gatcgatcaa cgtgctcttc cccgtgccgg
     1441 gataacccgt gatgccgatc accatggcgc gcttctccgg tggcgtcagg cgtttcaaca
     1501 cggcggctcc aaccggctcc tggttctcaa tccaggtgat cacgcgcgcc acagcc
//
LOCUS       DAORQN010000311         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1945_length_1548_cov_7.303416, whole genome shotgun sequence.
ACCESSION   DAORQN010000311 DAORQN010000000
VERSION     DAORQN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_1548_cov_7.303416"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /gene="tssD"
                     /locus_tag="PK614_10140"
     CDS             complement(<1..295)
                     /gene="tssD"
                     /locus_tag="PK614_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HRB82604.1"
                     /translation="MPIPAYLTVEVEKQGKIEGSCDLKGREGAILVQAFDHEVRIPSD
                     PQTGLASGKRVHGPLKIVKQVDKASPLLYQALCTGEHLKSVEIKWYRIAKDGTE"
     gene            complement(313..>1548)
                     /gene="tssC"
                     /locus_tag="PK614_10145"
     CDS             complement(313..>1548)
                     /gene="tssC"
                     /locus_tag="PK614_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRB82605.1"
                     /translation="ILHHATFQKLESAWRGLKLAVERTNFRENIQFEILNVSKEELLT
                     DFVEAPDVTKSSLYKHLYTAEFGQFGGNPIGAMIANYEFGPGPQDIKLLQHIASVATM
                     AHAPFVAAAGPKFFGMESFLRLPNLRDLKTHFEGPQYIKWNSFRDSEDSRSVGLCLPR
                     FLLRLPYSQETNPTKVFNYSESLSYGHESYLWGNTAFAFATRLTESFAKSRWYTNIIG
                     PISGGTVENLPVHLFESMGGIETKIPTEILISGEREKELADAGFISLTMRKGSDNACF
                     FSANSCQRPKTFGQSPEGKAAEANYRLGTQLPYLFIVNRLAHYLKVLQTEQLGGTKDR
                     ADLERELNTWISQYVSAQDVVTPEVRRRRPLRDAQVTVSDVEGNPGWYRVDLKVVPHI
                     KYMGAFFTLSLVGKLDKKK"
BASE COUNT          309 a          445 c          420 g          374 t
ORIGIN      
        1 cctcggttcc atctttcgcg atacggtacc atttgatctc gacggatttg agatgttctc
       61 cggtgcagag cgcctggtac agaagcggcg aggccttgtc cacctgcttt acgattttga
      121 gggggccgtg cacgcgcttt cccgaagcaa gccccgtctg cggatcggat gggatgcgca
      181 cttcatggtc gaatgcttgg accaggatcg ccccttctcg acctttcagg tcgcatgacc
      241 cttctatttt gccttgtttt tctacttcga ctgtcaaata ggctggaatc ggcattgtct
      301 ctccctcctc ttctatttct tcttgtcaag cttcccgacc agggacagcg tgaagaacgc
      361 gcccatatac ttgatatgtg gcactacttt taagtccacc cgataccagc ccggattgcc
      421 ctcgacatcg ctgaccgtca cttgggcatc ccgtaaaggg cgccgtctcc gcacttccgg
      481 agtgacgaca tcttgcgcgg acacatactg gctgatccag gtattgagct ctcgctcaag
      541 gtccgcccga tcctttgtgc cgcccagctg ttcggtctgg agcactttga ggtaatgggc
      601 caatcggttg acgatgaaga ggtagggcag ctgagtgccc agcctataat tcgcctcggc
      661 ggccttgcct tccgggctct gaccgaacgt cttgggccgc tggcaggaat tagccgagaa
      721 gaaacaggca ttatccgatc ctttccgcat ggtcaacgag ataaacccgg catcggccaa
      781 ttccttctcg cgttcgccgg aaataagaat ctcggtcgga atcttcgttt caatcccacc
      841 catggactca aagaggtgaa ccggcaagtt ctctactgtc ccgccgctga tcggaccgat
      901 gatgttcgtg taccaacgtg acttcgcgaa actttccgtc aggcgcgtgg cgaacgcaaa
      961 ggctgtattg ccccacaaat agctctcgtg gccgtagctc agcgactcgc tgtaattgaa
     1021 gaccttggtg ggattcgtct cctggctata cggcaaccgc aacaggaacc gaggcaggca
     1081 gagtccgacg gatcgcgaat cctcggagtc ccggaacgaa ttccatttga tgtactgtgg
     1141 accctcgaaa tgggtcttta agtctctgag attggggagc cggaggaaac tctccatgcc
     1201 gaagaacttt ggcccggccg ccgcgacaaa gggtgcatgc gccatggtgg ccacgctcgc
     1261 gatgtgctgc aggagcttga tatcttgcgg accaggaccg aattcgtaat tcgcgatcat
     1321 cgcccctatg gggttgcctc cgaattgtcc gaactcggcc gtgtacaggt gcttgtacag
     1381 gctggacttg gtcacgtccg gtgcttcgac aaagtcggtc aggagttctt ccttcgatac
     1441 gttcagaatc tcgaactgaa tgttctcccg gaaattggtc cgctctactg ccagcttcag
     1501 accacgccag gcggattcga gcttctgaaa cgtggcatgg tgcaggat
//
LOCUS       DAORQN010000312         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1960_length_1538_cov_4.380984, whole genome shotgun sequence.
ACCESSION   DAORQN010000312 DAORQN010000000
VERSION     DAORQN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_1538_cov_4.380984"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..546
                     /locus_tag="PK614_10150"
     CDS             160..546
                     /locus_tag="PK614_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82606.1"
                     /translation="MTRYILAAIAGIWMADGLALLTVPLLVIKRVQESLLHSPQLLRW
                     EAVGIGLGAILILWSGPIPYQPLWWITGGAMIIKGCFLTWGPAAWRTPLLDWCFAREA
                     IDYRFFGLWLCMLAVLLLHALGLLHR"
     gene            584..877
                     /locus_tag="PK614_10155"
     CDS             584..877
                     /locus_tag="PK614_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82607.1"
                     /translation="MSHQTIASLWEEHSREGWPRFSSPNEGQLMTLDTVIGGCAVFYL
                     DGQMEIDSQRIAILQDCVADLDTLLDDLTEDSLGYFQRLRRLAAALVQLNQAT"
     gene            complement(938..1396)
                     /locus_tag="PK614_10160"
     CDS             complement(938..1396)
                     /locus_tag="PK614_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HRB82608.1"
                     /translation="MGAIVLSALAVLAGCADTGSLNGAGRQAVGATATKSLYDRLGGK
                     PAITAVVDQFVANVAADGRINSRFATTDIPKLKGHLVDQVCGATGGPCSYRGRDMKST
                     HLGMQISSADFGALVQDLGAALDKFNGPAGEKSELLGILGPMKKDIVEVP"
BASE COUNT          291 a          519 c          417 g          311 t
ORIGIN      
        1 tcaccagtgc cttccatcga accctgcagt tctcagtgga gaaacggacg acgatgcgca
       61 tggcggcgtt gatgtcgggc atcgacaagg tggcccaggc gcatctccaa cgcgggctct
      121 acccttagac gacaggatgc cgaagacctc gctcatgcca tgacacggta catactcgcg
      181 gcgatcgccg gaatctggat ggccgacggc ctcgccttgc tgacggtccc cctgctcgtc
      241 atcaaaagag tccaggagtc cctcctgcac agcccacaac tgctccgctg ggaagccgtc
      301 ggtatcggcc tcggagccat tctaattctc tggtctggac cgattcccta tcagccgctg
      361 tggtggatca ccggcggcgc catgatcatc aaggggtgct tcctcacatg ggggcccgcc
      421 gcatggcgca cgccgctgct ggattggtgt ttcgcccgcg aagccatcga ctaccggttc
      481 ttcgggctgt ggttgtgcat gcttgccgtt ctgctgctgc atgcgttagg gttgcttcac
      541 cggtaactac ggcttacagc gggcctcgat cgagaccatc gtcatgagtc accaaaccat
      601 cgcctcactc tgggaagagc acagccgcga gggctggccg cgtttttcca gcccgaacga
      661 aggacaattg atgacgctgg ataccgtcat cgggggatgt gccgtgtttt acctcgacgg
      721 gcagatggaa atagacagcc aacgcatcgc gatcctccag gactgcgtcg ccgacctgga
      781 taccttgctc gatgacctga ctgaagacag cttgggctac tttcaacgac tccgccgact
      841 cgcggccgcc ctcgtccagt tgaatcaagc gacctaaccg cgacaccgcg aggcgcagcg
      901 gggaccgacc ggtaggcccg cccccgctgt ctgttcccta cggcacctcg acaatatcct
      961 tcttcatcgg accgaggatc cccaacaatt cgcttttctc accggccggc ccgttaaact
     1021 tgtcgagcgc ggcgccaaga tcctgaacca gggctccaaa gtccgcactg ctgatttgca
     1081 tccccaggtg cgtgcttttc atgtcgcggc cacgatagct gcaggggcca cccgtcgcgc
     1141 cgcacacttg atcgaccagg tgccccttca acttggggat gtccgtcgta gcaaagcggc
     1201 tattaatccg cccatccgcc gccacgttgg ccacaaattg atcgaccacc gccgtaatgg
     1261 caggctttcc tcccaaacgg tcgtagaggg actttgtggc cgtcgcaccg accgcctggc
     1321 gtccggctcc gttcagactc ccggtatccg cgcaaccggc cagcaccgcc agtgcactga
     1381 gtacaatcgc tcccattcca acacttttcg ataccatcat ccgcccccct tgtaatatgt
     1441 gaccgactgc tggccctcct cgacccacca tgagccgggg ttttcaacca gccgcaccgg
     1501 ttgaccccag acgatagggg agatatgatc tattcgtc
//
LOCUS       DAORQN010000313         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1972_length_1527_cov_3.908967, whole genome shotgun sequence.
ACCESSION   DAORQN010000313 DAORQN010000000
VERSION     DAORQN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_1527_cov_3.908967"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..823)
                     /locus_tag="PK614_10165"
     CDS             complement(146..823)
                     /locus_tag="PK614_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB82609.1"
                     /translation="MALALQAILAEAQVIKAGPASCPAVALTYDLCPVRTASGFDREL
                     IDFLIEHKVPATFFMSGRWMAKHDAEVKKLLAVPFFEIGTHGEVHAHLPMHEVEEQQQ
                     EIMGPVRLLKTKYGRPATLFRPPYGEYNDQTVDAVKTLGLKFILWNIESGDPDPTLAA
                     EAIVARVQKRMKPGSVIVMHANGKGKHTREVTETLAATLLPAKGLTPMTVSDLLRCNQ
                     STVTPAP"
     gene            941..>1527
                     /locus_tag="PK614_10170"
     CDS             941..>1527
                     /locus_tag="PK614_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB82610.1"
                     /translation="MQGPDWKIGRMFGIPIHVHASWLFVFFFVTWSLATGYLPDVLPG
                     LTEPRYWAMGGVAAILLFASVLLHELGHSLVALRYRIPISQITLFIFGGVAQMRKEPP
                     HPRAEFLIAIAGPIVSFLLAGLCIGLVALLEFLPTGSSVHGLVALGTLLGMVNTQLGL
                     FNLLPGFPLDGGRALGAGLWAWSKDFYRATSQAALV"
BASE COUNT          248 a          442 c          495 g          342 t
ORIGIN      
        1 tccagtacga atgtgtctcg tcccgcgggc agcggcgcaa agatgcggtt ccaatcggcg
       61 gcgttgaagc cgagccgttt ccaggggtcg gagctggtca aaatcccgac cacgggatcg
      121 cgatcctccg ctcgcatgtc gcgaattatg gtgcgggtgt gactgtcgat tggttgcatc
      181 gtaagaggtc cgacacggtc atcggggtca ggcctttggc cggcaacagg gtggcggcca
      241 gggtttcggt cacttcgcgg gtatgtttgc ccttgccgtt ggcatgcatg acgatcacgc
      301 tgcccggttt catacgtttc tgaacacgag ccacgatcgc ctctgccgcc agtgtcgggt
      361 cgggatcgcc ggactcgata ttccacagga tgaacttgag cccgagagtt ttcacggcgt
      421 caaccgtttg gtcgttatat tctccatagg gcggccggaa tagggtggcc gggcgaccgt
      481 atttggtctt gagcaggcgc accggtccca tgatttcctg ttgctgttcc tcgacctcgt
      541 gcatcggcaa atgggcatgc acctcgccgt gggtgccgat ttcgaaaaac ggcacggcga
      601 gcagcttctt gacctcggca tcgtgcttgg ccatccagcg gccggacatg aaaaacgtcg
      661 ccggcacctt gtgctcgatg aggaaatcga tgagctctcg gtcgaacccc gacgccgtgc
      721 gcacggggca aaggtcatag gtcaaagcca cggcagggca ggaagccggc cccgccttga
      781 tgacctgcgc ctccgcgaga atagcctgaa gggcgagtgc cattccgcag atcgccgaca
      841 gtctgatcca gaactgcttg ccacgcatgg gcgcagtata cccgaaggca cgaagcggcg
      901 atagttgacg ctctgtccgg gtggctgcta cggtgaaacc atgcagggac cagactggaa
      961 aatcggccgg atgttcggga ttcccatcca cgtgcacgcc tcgtggctct ttgtcttttt
     1021 ctttgtgacc tggtcgttgg cgacgggcta tttgcccgac gtgctgcccg gtctcaccga
     1081 gccgcgatat tgggcgatgg gcggtgtggc ggccatcctc ctgtttgcgt ctgtcctcct
     1141 gcatgagctc ggccattccc tggtggccct tcggtatcgg attccgatca gtcagatcac
     1201 gctgtttatc ttcgggggtg tggcgcagat gcgaaaggaa ccgccgcatc cccgggctga
     1261 gttcttgatt gcgattgccg gcccgatcgt gagctttctt ctcgccggtc tgtgcatcgg
     1321 cctggtcgcg ctgttggagt tccttccgac aggatcctcc gtgcacgggt tggtggcgtt
     1381 ggggacgctg ctcgggatgg tcaacactca gctgggcctg tttaatctgc tcccgggctt
     1441 cccactggac ggcgggcgcg ccttgggggc cgggttgtgg gcctggagta aggacttcta
     1501 tcgcgcgaca agccaggcgg cattggt
//
LOCUS       DAORQN010000314         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1988_length_1508_cov_10.960083, whole genome shotgun sequence.
ACCESSION   DAORQN010000314 DAORQN010000000
VERSION     DAORQN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_1508_cov_10.960083"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PK614_10175"
     CDS             complement(<1..572)
                     /locus_tag="PK614_10175"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82611.1"
                     /translation="MNLKEDLILIGGGGHCRSCIDVIEMEGRFAIRGIVDEKDALDPT
                     LTDYPLLGREEDIPTLSKACHNFLITIGQIKSPEPRIRLFLRLKQLGMTLPIVVSPLA
                     HVSRRARLGEGTIIMHDALINAGASVGRNCIINTKALIEHDAVIEDHCHISTAAIVNG
                     AAMVRRCSFIGSNADDVYFFSREYFEELTA"
     gene            complement(613..1275)
                     /locus_tag="PK614_10180"
     CDS             complement(613..1275)
                     /locus_tag="PK614_10180"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB82612.1"
                     /translation="MKSDDATSQAQPSRGFLAKRWFDVIVTLSVMPVALPAFLLVALA
                     VRCGIGSPILFKQQRPGLYGSPFTLFKFRTMTDARDRDGQLLPDSQRLTRVGRFLRST
                     SLDEIPELLNVLKGDMSLVGPRPLLMEYLAHYTPEQQRRHLVPPGLTGWAQVNGRNTA
                     NWQQRFLLDLWYVEHRSMWLDCKILLLTPWKVLKREGMMHPDDPSWEGNFKGASSDRP
                     SR"
BASE COUNT          340 a          417 c          440 g          311 t
ORIGIN      
        1 ttcgctgtca actcctcaaa gtattcgcgc gaaaagaagt agacgtcgtc cgcattgcta
       61 ccgatgaacg aacaacgccg gaccatggcc gcgccgttca cgattgcggc cgtagagata
      121 tggcaatgat cttcgatgac agcgtcgtgc tcgatcagag ccttggtatt gataatgcaa
      181 ttgcgtccaa cagaggcccc cgcgttgatg agcgcgtcgt gcatgatgat agtcccctcc
      241 ccgagcctgg cgcggcgaga aacgtgggcc agaggagaga cgacgatggg gagggtcatg
      301 ccaagttgtt tcaggcgtaa aaagagtcga atgcgcggct ctggagactt gatctgtccg
      361 atggtgatca gaaagttgtg gcaggccttc gacagcgtag gaatgtcttc ctcccgaccc
      421 agcagcgggt agtccgtgag cgtgggatcc aacgcgtctt tttcgtcaac gatgccgcgg
      481 atggcgaagc ggccctccat ctcgatgacg tcgatgcagg agcggcaatg cccgcccccg
      541 ccgatcagaa tgaggtcttc cttgagattc atgtatacat ttccgatgca acatgtgccg
      601 agatacggcg tactatcgag aaggccggtc agacgaggcg cctttgaaat tgccttccca
      661 ggatgggtcg tctggatgca tcatgccttc ccgcttcaag actttccaag gtgtcaacag
      721 caggatctta cagtccaacc acatcgaacg gtgttccacg taccataggt cgagaagaaa
      781 tcgttgttgc caattggcgg tgtttcgccc attcacctgg gcccaccctg taagtccagg
      841 gggaacgagg tgacggcgct gctgttccgg agtgtaatgc gcgagatatt ccatcagcag
      901 aggccgaggg ccgacgaggc tcatatcacc cttgagtacg ttcaaaagct caggaatttc
      961 gtccaggctg gtggagcgca agaaccggcc gacgcgagtc aaccgttgag aatccggaag
     1021 cagctgtcca tcgcgatcgc gcgcatcggt catggtgcga aatttaaaca gggtaaaagg
     1081 cgatccgtac aaacccggtc gctgctgttt gaacaggatc ggggagccaa tgccgcatcg
     1141 aactgcgagc gcaacaagga gaaacgcagg cagggcgacg ggcatcacgc ttagggtcac
     1201 gatgacatca aaccaccgtt ttgccaggaa gccgcgtgac ggctgtgcct gcgatgtcgc
     1261 gtcatcagat ttcatgaatc gcgctccccg ttggtcgtgc tccgcgaccg cgcccattgc
     1321 ctatggctca agccgtttgg cgcgtcctac gaatgggacg gccccaacgg ttcctactcg
     1381 cgacacaccc tgtctttcag ggtagcgata ccgagggaaa aaattgcaag gctccaggcc
     1441 ggatatccgt tgcgccgatt tggcggtaag atgcaatctt gcattcttct cctcatccat
     1501 aacgacgc
//
LOCUS       DAORQN010000315         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_1994_length_1505_cov_3.138621, whole genome shotgun sequence.
ACCESSION   DAORQN010000315 DAORQN010000000
VERSION     DAORQN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_1505_cov_3.138621"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..>1204)
                     /locus_tag="PK614_10185"
     CDS             complement(134..>1204)
                     /locus_tag="PK614_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRB82613.1"
                     /translation="AIGLAIMNTWQPGLAWQGRVDALLQLVPGTKPSATVLANVQAGS
                     QSPIEYLASYIPRTITDPFSSNNIIGVVLLALVLGVTLRMVRSEKDQTTGLVNALVRG
                     IEWVYVWLVQILEWIILVVPLAVFGVVAQVVGKAGIGVFSVLWIFLVAMLAGLAAHAL
                     VYYPLLAWLVGKKSPKVYLGQGADAILTAVSCNSSLATVPVTLQCLHRMNVSPQSSRL
                     AACVGTNLNNDGITLYEAMAALFLAQALGFDLPMVKQVLIVLASIIAGAGVAGIPEAG
                     LIVLPLVLAAAGLPDHVIIAAIPLIMTVDWIIARARSGVNVMSDMLVAILLDAGHVAP
                     AAEPAAIPRPSDTTHPTEPQIF"
     assembly_gap    1205..1254
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1255..>1505)
                     /locus_tag="PK614_10190"
     CDS             complement(<1255..>1505)
                     /locus_tag="PK614_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRB82614.1"
                     /translation="PLEIDMKPTHRWIPLPLYTQVLIAVICGGLLGALFGQEPYWGGL
                     RNAQLGKLGLFVVTLLKTLAIPLIFFAILDALIRTTIPQR"
BASE COUNT          288 a          413 c          518 g          236 t
ORIGIN      
        1 catggggcgt aaggctggcg gcaggagagg aggaaatgcc cgttcattcc gcctgatggc
       61 ctcgcggata ttctggaggt gctgcagctt tggagcagcc tcaaggtggc cttgcgctca
      121 ttgtctgcgc tcatcagaaa atctggggct ccgtgggatg agtggtatcg gacgggcgag
      181 gaattgcggc cggttcggcg gcaggcgcga cgtgcccggc atcgagcagg atggcgacga
      241 gcatgtcgct catgacgttc acgccggagc gggcgcgggc gatgatccaa tccaccgtca
      301 tgatgagtgg aatggccgca atgatgacgt gatccggaag gcccgcggca gccaacacca
      361 gtggcaggac aatcagtccg gcttcgggaa tgcccgccac gccggccccc gcgatgatgg
      421 aggcgagcac gatcagcacc tgtttcacca tcggcaggtc gaagcccagc gcttgcgcga
      481 gaaagagggc ggccatggct tcatagaggg tgatgccgtc gttgttgagg ttcgtaccga
      541 cgcaggctgc gaggcgcgac gattgcggcg agacgttcat gcgatggaga cactggagtg
      601 tcaccggcac ggtcgcgagg ctgctgttgc aggacacggc ggtcaggatg gcgtcggcac
      661 cctgacccag gtagaccttc ggcgacttct ttcccaccag ccaggcgagg agcggataat
      721 acacgagcgc gtgggccgcg agtcctgcga gcatggcgac gaggaagatc cagagcacgg
      781 aaaagacgcc gatgccggcc ttgcccacga cctgcgcgac gacgccgaag acggcgagcg
      841 gcaccaccag gatgatccat tcaagtatct gcaccagcca gacatagacc cactcgatgc
      901 cgcgcacaag ggcattgacc agcccggtgg tctggtcctt ttcgctccgg accatccgca
      961 gggtgacgcc gagcaccagc gcgaggagga cgacgccgat gatgttgttg ctcgagaacg
     1021 ggtcggtgat ggtgcgggga atgtaggagg cgaggtactc gatcgggctt tgagacccgg
     1081 cctgcacgtt ggccagcacg gttgcggagg gcttggtccc cggcaccagt tggagcaggg
     1141 catcgacgcg gccctgccag gccaggcccg gctgccaggt gttcatgatc gcgaggccga
     1201 tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgctgc
     1261 ggaatggtgg tgcggatgag cgcatcgagg atcgcgaaaa agatcaacgg aatggcgagt
     1321 gtcttgagca gggtgacgac gaagagaccg agtttgccca gctgcgcatt gcgcagaccg
     1381 ccccaatagg gctcctgccc gaagagggcg cccagcagcc cgccgcagat cacggcaatg
     1441 agcacttgtg tgtagagcgg cagtgggatc cagcgatgcg tcggcttcat gtcgatctct
     1501 agtgg
//
LOCUS       DAORQN010000316         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2019_length_1486_cov_5.007687, whole genome shotgun sequence.
ACCESSION   DAORQN010000316 DAORQN010000000
VERSION     DAORQN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2019_length_1486_cov_5.007687"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>1170)
                     /locus_tag="PK614_10195"
     CDS             complement(44..>1170)
                     /locus_tag="PK614_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRB82615.1"
                     /translation="YHLLAELGTEASRGLDRILLYEGSRGCWWGEKHHCTFCGLNAQS
                     MKFRAKSSEQVAREMAYLSSRYDTTRFRLVDNIIDMKYVENLFGRFAAEHCDLDVFIE
                     TKSNLQKSQIRTLAVGGVKCMQPGLESLSLSQLKAMDKGVTPMQNIICLKWSCYYRVS
                     VSWNILLGFPGETNEDYRRQIDLLPSLFHLQAPEASGKFWLQRFSPYFTRPHEYGVRI
                     TGPGTAYEYVYDAARVDLRKIAYDFEYELDDWPVDPHVYQELIGLVEEWQRLARSSDR
                     PFLYYSKAMEYVTIYDGRNPQMPTRRRYDWPAAGIIEACNEAAKSREQIRAALSEATR
                     DRVCSEDELTEALGILTSRRILYEERGKYFTLAIPEHPYY"
     assembly_gap    1171..1197
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1198..>1486)
                     /locus_tag="PK614_10200"
     CDS             complement(<1198..>1486)
                     /locus_tag="PK614_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM protein 1"
                     /protein_id="HRB82616.1"
                     /translation="ASLTMAKLIKDLYPDVKIVFGGANYDGEMGLEYFRAFPFIDHVV
                     VGEGEVTFPALVTHILSGSAGPCPKGVTYREQGDIRCEPNTALFTEFAQTGP"
BASE COUNT          315 a          459 c          383 g          302 t
ORIGIN      
        1 atgtagggga ggggagaagg acggaagaga ccgcattatc cgctcagtag taggggtgct
       61 cgggtatggc cagcgtgaaa tacttcccgc gctcttcata cagaattcga cgactcgtca
      121 agatgccaag cgcctcggtc agctcgtcct ccgaacatac cctgtcacgc gttgcctcac
      181 tcagcgccgc gcgaatctgc tcccgactct tcgcagcctc gttgcacgcc tcgatgatgc
      241 ccgccgcagg ccagtcgtac cgtcgtctcg tgggcatctg tggattacgc ccgtcataga
      301 tcgtgacata ctccatggcc ttggagtaat acaaaaacgg ccggtcgctg ctgcgcgcca
      361 gccgctgcca ttcctccacc aagccgatca attcctgata gacgtgggga tccaccggcc
      421 agtcgtcgag ttcatactcg aaatcatagg cgatcttgcg caggtcgacg cgcgccgcat
      481 catacacata ttcataggct gtccccggac ccgtgatgcg cacgccgtac tcatgcggtc
      541 tcgtaaagta gggactgaac cgttgcaacc agaatttccc gctcgcctcc ggcgcttgca
      601 aatggaatag ggacggcagg agatcgatct gccgccgata gtcctcattc gtctcgccgg
      661 gaaagccgag cagaatattc caggataccg acactcggta gtaacaactc cacttgaggc
      721 aaatgatgtt ctgcatgggc gtcacgccct tgtccatggc cttcagctgt gacaggctga
      781 ggctttccaa accgggctgc atgcacttca cgccccccac agccagcgtc cggatctggc
      841 tcttctgcag attactcttg gtttcgataa acacatccag atcgcagtgc tcggcggcaa
      901 accggccaaa caggttctcg acatatttca tgtcgatgat gttgtccacc agccggaacc
      961 gtgtcgtatc gtagcgactc gacagatacg ccatctcgcg agcgacctgt tcagacgact
     1021 tggcgcggaa tttcatactc tgcgcattca gcccgcagaa ggtgcaatgg tgcttctcac
     1081 cccaccaaca accgcgtgag ccttcgtaca atagaatacg atctaggccg cgcgacgcct
     1141 cggtgccgag ttcggccagc agatgataat nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     1201 ggccggtctg tgcgaactcc gtgaagagcg cggtattcgg ctcacatcga atgtcgccct
     1261 gctcccgata cgtcacccct ttcgggcagg gcccggccga tccgctcaag atatgggtca
     1321 ccaatgcggg gaacgtcacc tcaccttcac ccacgaccac atggtcgatg aacggaaagg
     1381 cgcgaaagta ttccaatccc atctcgccgt catagttggc gccaccgaac acgatcttca
     1441 cgtccggata caaatccttg atgagcttcg ccatggtcag actggc
//
LOCUS       DAORQN010000317         1481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2024_length_1481_cov_2.512623, whole genome shotgun sequence.
ACCESSION   DAORQN010000317 DAORQN010000000
VERSION     DAORQN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2024_length_1481_cov_2.512623"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..634
                     /locus_tag="PK614_10205"
     CDS             65..634
                     /locus_tag="PK614_10205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRB82617.1"
                     /translation="MITVMDACPTLRTERLLLRQFQPSDADTVHRLAGVKEVAAGTLL
                     PHPFDVEAAAQWIAQQLEHFAASNALTFAIVLTEEEQLIGSIGMEIARKHQLARLSYW
                     LGTAYWNQGYCTEAVRAVLDYGFTRLSLHRIYAPHFHNNPASGRVLRKVGMTYEGRMR
                     EHYIRFGQFVDVEIYGMLREEFLHGRAHK"
     assembly_gap    735..744
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<745..1225)
                     /locus_tag="PK614_10210"
     CDS             complement(<745..1225)
                     /locus_tag="PK614_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82618.1"
                     /translation="MYSVQGGKHLPDAFMFAGAGCIGLFGCYRLWRANGSWKWTLVPV
                     AVIFFAVAFPNFERGHHGTPVERASFSALQRLSAFAGSATQLAREQGEFVCDRSATLS
                     ASSLFARQGQALSYVIHCVPDATGPTLGTPPERPGTLVFAVSPDRKQAWFTATVLPHS
                     "
BASE COUNT          339 a          438 c          421 g          273 t
ORIGIN      
        1 cctttatgac gcgcatcgcg gtcgacgccg gacgcggcag ttgttttagg ctggatacta
       61 tgctgtgatc accgtgatgg atgcctgtcc gacactccgt actgaacggc tgcttctccg
      121 gcaattccag ccctccgatg ctgataccgt gcaccgcctg gcaggggtga aggaagtcgc
      181 cgcaggcacg ttgctccctc acccctttga cgtcgaagcc gccgcacaat ggatcgctca
      241 acaactggag catttcgccg caagcaacgc cctcacattt gccatcgtcc tcactgaaga
      301 ggaacaactc atcgggtcaa tcgggatgga aatcgcccgc aagcaccaac ttgcgcgcct
      361 gagttactgg cttggcaccg cctactggaa ccagggctac tgcaccgaag cggttcgggc
      421 ggtgctcgac tacggcttca ctcgcctgtc actccatcgc atctatgccc cgcactttca
      481 caacaacccg gcctcgggac gtgtcttacg aaaagtcggg atgacctacg aaggccgcat
      541 gcgcgagcat tacatccgct tcggccaatt tgtggatgtg gagatctatg ggatgctgag
      601 ggaggaattc ttgcacggcc gcgcgcataa atgaggccat ggtgtcggca tacttggata
      661 gtggcaagaa gacgagaagg gaaaccagcc acatacgttg ggctaggatt tagattgtag
      721 ctgtgccgca atgannnnnn nnnntgctgt gaggcaacac cgtagcggtg aaccaggcct
      781 gcttccgatc cgggctgacg gcgaagacga gggtgccggg tcgttcggga ggcgtaccga
      841 gtgtcggccc ggtcgcatcg ggcacacagt ggatcacata ggaaagagcc tggccttgtc
      901 gagcaaacag agacgaggcg gagagcgttg cggatctgtc acagacaaat tccccctgct
      961 cccgggcgag ttgagtcgct gaaccggcga acgcactcaa cctttgcagc gcgctgaatg
     1021 acgcccgctc caccggtgtt ccgtgatgcc cacgctcaaa attcggaaag gccaccgcaa
     1081 agaaaatcac cgcaaccggt accagggtcc atttccaact tccattagcc cgccacagtc
     1141 gataacagcc gaacagtccg atgcagcccg caccagcgaa catgaatgcg tcggggagat
     1201 gtttcccgcc ctgaactgag tacatcgacg gatcatgagc ctgaacctcc ctcaggaccg
     1261 ataccacccc cgccccaatg agtatgaggg gcaagaatcc caacagaatg gaagcgattc
     1321 gtctgacgag cagcataggc tactgccagc atatcgcgtt tggaaatggg ggccaggaaa
     1381 tagggagatg gtgcgggggc tctgagcacg atgtggcagt tgcaaacgaa ggcgagacga
     1441 caaaaccctc cccttctgcc gccaacttat ccaagtccct c
//
LOCUS       DAORQN010000318         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2030_length_1477_cov_5.542194, whole genome shotgun sequence.
ACCESSION   DAORQN010000318 DAORQN010000000
VERSION     DAORQN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_1477_cov_5.542194"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="PK614_10215"
     CDS             complement(<1..1463)
                     /locus_tag="PK614_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HRB82619.1"
                     /translation="MNPDHLGTKAAAQYGLTLAPGETQTVRLRLTDDGRSPEFVEQDF
                     EAVFAARIREANEFYDGLAPAHLSEDARRIQRQAFAGLLWSKQFYHYDVNRWLKGDPA
                     GQSPPRERLHGRNSDWTHLYNADVVSMPDKWEYPWYAAWDLAFHCIPLALVDPTFAKE
                     QLVLMLREWYMHPNGQIPAYEWAFGDVNPPVHAWAAWRIYKIDKKRNGVGDRRFLERV
                     FHKLLLNFTWWVNRKDAEGKNIFQGGFLGLDNIGVFDRSAPLPTGGHIEQSDATSWMG
                     MYCLNMLTIALELARDNRAYEDVASKFFEHFVYICRAMNNIGGENIELWDREDGFFYD
                     VLHLPTGETRHMKVRSMVGLIPLFAVETLDSELVDSLPRFKHRMQWFIENRPDFAQHL
                     ETQSCDGGVRRFLSLVGRDRLKSVLRYMLDEEEFLSPYGIRALSRYHRDHPFVMSVMG
                     REHRADYEPAESGTGLFGGNSNWRGPIWFPVNYLLIES"
BASE COUNT          306 a          454 c          430 g          287 t
ORIGIN      
        1 gactcgatca ggagataatt gaccgggaac cagatcggcc cgcgccaatt cgaattgcct
       61 ccgaacaagc ccgtgccgga ttccgccggt tcgtagtccg cgcgatgctc acggcccatg
      121 accgacatca caaagggatg atcgcggtga taacgcgaga gcgcgcggat gccgtatgga
      181 gacaggaact cctcttcatc cagcatgtac cgcaacacgg atttcaggcg atctcggccg
      241 acaagcgaga gaaagcgccg cacgcctccg tcgcaggact gcgtttccag gtgctgcgca
      301 aagtccggcc ggttctcgat gaaccactgc atccggtgct tgaagcgcgg caggctgtcc
      361 acgagttctg aatcgagcgt ctcgaccgca aagagaggaa tcagacctac catcgagcgg
      421 accttcatgt gccgggtttc cccggtcggc agatgcagca cgtcgtagaa gaagccgtct
      481 tcacgatccc acaactcgat gttctcaccg ccgatgttat tcatggcgcg acagatgtag
      541 acgaaatgtt cgaagaattt gctggccacg tcttcatagg cgcgattgtc ccgggccagt
      601 tccagcgcga tcgtcagcat gttgaggcag tacatgccca tccagctggt cgcatccgac
      661 tgttcgatgt ggccgccggt cggcagcggc gcgctccggt cgaatacccc gatgttatcg
      721 aggccgagaa acccgccttg aaagatgttc ttgccctcgg cgtctttgcg attcacccac
      781 caggtaaaat tcaacaacag tttgtgaaag acgcgttcca aaaaccgtcg atcgccgacg
      841 ccgttccgct tcttgtcgat cttatagatg cgccaggccg cccaggcatg gaccggggga
      901 ttcacgtcgc cgaatgccca ttcataggcc ggaatctgac cgttggggtg catataccat
      961 tcgcgcagca tgaggacgag ctgctccttg gcgaacgtcg ggtccaccag cgcgagcggg
     1021 atgcaatgaa acgcgagatc ccaggcggca taccagggat actcccactt atccggcatc
     1081 gagacgacat cggcgttata gaggtgcgtc cagtccgagt tccggccgtg gaggcgttca
     1141 cgcggggggg actgccctgc cggatcgccc ttgagccatc gattcacatc gtagtgatag
     1201 aactgtttgc tccagagcag tccggcaaag gcctgccgtt gtatgcggcg cgcgtcttcc
     1261 gacagatggg ccggggcaag gccgtcgtag aactcgttcg cttcacgaat gcgcgccgca
     1321 aagacggcct cgaagtcctg ctccacgaac tccgggctcc ggccatcatc ggtcaatcgc
     1381 aagcggacgg tctgtgtttc accgggcgcg agcgtgagcc catactgagc cgcagctttg
     1441 gtccccagat gatccggatt caccgcctcc ttctcac
//
LOCUS       DAORQN010000319         1467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2040_length_1467_cov_4.698300, whole genome shotgun sequence.
ACCESSION   DAORQN010000319 DAORQN010000000
VERSION     DAORQN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_1467_cov_4.698300"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PK614_10220"
     CDS             <1..1103
                     /locus_tag="PK614_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRB82620.1"
                     /translation="TGDRNGSVAWPPPGHVYLVANLKDLGLSALGNFTAGIGMNNPFG
                     SLTRYPNDGPFRTAITFTTLPLLDIKPTIAYKLNDQLSFGLGADIYTFSGLFGEGHLE
                     QKSIWPGGLGIPAGSLMEINGSDTTAGFNVSLLYTPFRNSDGKPLVNIGMVYRSQATF
                     HLTGNFLANGASVAGATATFVLPQVFSGGIGIWPVRTSEREWKLEFDVDYTGWKSNRN
                     LDVHLTNGALLPFPQNWQSTYTVMIGTEYRWLRLASMPDWDIALRAGYMNQQAQIPDR
                     TFNPGVPSANTHIPSVGIGLACHENGSFLGLVRCGELGIGPLKPKLFAIDLAYQAGLY
                     EVRTIAGNQNPTVNGRYDSMVHVGSLSLRFNY"
BASE COUNT          299 a          478 c          380 g          310 t
ORIGIN      
        1 tgacaggcga tcgcaacgga agcgtggcct ggccccctcc cggccacgtg tacctggtcg
       61 cgaatctcaa ggacctcgga ctgtcggcgc tcgggaactt caccgccggt atcgggatga
      121 acaatccgtt cggttcattg accagatatc cgaacgatgg gccgtttcgc acggccatca
      181 ccttcaccac cttgccgtta ctcgatatca aacccaccat cgcctacaaa ctgaacgacc
      241 aactctcgtt cggattgggc gcggatattt acaccttttc cgggctcttc ggagaaggcc
      301 atctcgaaca gaaatccatc tggcccggcg ggctgggcat cccggccggc tccctgatgg
      361 agatcaacgg aagcgacacc acggcaggat ttaacgtcag cctcctgtac acccccttcc
      421 gcaacagcga cgggaaacca ctcgtcaaca tcggcatggt ctatcgcagt caagccacct
      481 ttcatctcac gggcaacttt ctcgccaacg gcgcctcagt cgccggcgcc acagccacct
      541 tcgtcctgcc gcaagtcttc tccggcggca tcggcatctg gccggttcgt acgtccgagc
      601 gcgaatggaa actggaattc gatgtggact acacgggatg gaaatccaac aggaacctcg
      661 atgtccattt gacgaacggc gccctgttgc cgtttccgca aaattggcag agcacctata
      721 ccgtcatgat cggcaccgaa taccgatggc tgcgcctggc ctcgatgccg gattgggaca
      781 ttgcccttcg ggccggctac atgaaccagc aggcacaaat acccgatcgc acctttaatc
      841 cgggagtccc ctcagccaat acccacattc cctccgtcgg gatcgggttg gcctgccatg
      901 aaaacggttc gttcctcggg ctggtgcgtt gcggcgaact gggaatcggc ccgctgaagc
      961 cgaaattgtt cgccatcgat ctcgcgtatc aagcgggtct gtatgaagta cgcaccatcg
     1021 caggcaacca aaacccaacc gtcaatggcc gatacgacag catggttcat gtcggctcgc
     1081 tgtcgttgcg gttcaactac tgagtgcatc ccctctcgcg cagcgcggct ctcacattgg
     1141 tttcatttcc aggacgccga cggcacagcg gttgcttctc cctccgcatg caggtgttgt
     1201 ggacatgaac agtgtttcaa attgctcgac acgtatgtcg gcgtggtatc gagccggttt
     1261 cctgtcgccg gttagagagt agccggccgc atgtcacggc ctcctggatc gtgcccatcc
     1321 agcgctgatc gaaccgcctc cttccactcg accagccgcg tcttcgccat caacgaagac
     1381 gcggcgcctt ttcacccgaa agactttaga tagtcactcc ggatctgatc tgtcccgcca
     1441 tgtgacctga tcaatacgtc tttcgat
//
LOCUS       DAORQN010000320         1460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2048_length_1460_cov_4.366548, whole genome shotgun sequence.
ACCESSION   DAORQN010000320 DAORQN010000000
VERSION     DAORQN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_1460_cov_4.366548"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PK614_10225"
     CDS             <1..945
                     /locus_tag="PK614_10225"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRB82621.1"
                     /translation="GCNRNCAFCAIPIMRGKQRSRPIESIVAEAGRLVQEGVKELNLI
                     SQDTINYGVDLGLKQGLTALLRSLVTVKDVHWIRPFYLYPQQVTDELLDLYAGEARIT
                     KYLDMPLQHISDGMLKRMHRLGDRKHVTKLVERIRAKIPGVFFRTAFIVGFPGETDAM
                     FEELKQFILDMEFDRVAVFLYSDEEGTSAVDLDRKVDQAVMEERRNELLALQESISES
                     KNRDYLGRTMEVLVDGISEESDRLLECRHEGLAPEIDGVVFCDRSAARPGEFVSVTVT
                     DVAGYDLIAQPVGRTDAPISVQTAPTLLMPKKSGAGYR"
     gene            complement(993..>1460)
                     /locus_tag="PK614_10230"
     CDS             complement(993..>1460)
                     /locus_tag="PK614_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRB82622.1"
                     /translation="SFQGTPIEDTVTLQRAVTRSSVGSKATVKVMRDGREKDLTVTIA
                     ELPDNPQVAKAEAGPSEQPLAGLAVQELDRETAQELGIKGKIQGVVVTTVDPESDAER
                     AGLMPGDVIREINRKPVTSMKDFDRAASDLKKGQTVLVLVNRRGASLYLSAKI"
BASE COUNT          281 a          415 c          445 g          319 t
ORIGIN      
        1 ggctgtaacc ggaactgcgc gttctgtgcg attcccatca tgcggggcaa gcagcgcagc
       61 cgcccgattg aatcgattgt ggctgaagcg ggacggttgg ttcaggaagg ggtgaaggaa
      121 ctcaacctga tttcccagga cacgatcaat tacggagtgg atctcggact caagcagggg
      181 ctcacggcgc tgcttcgctc actggtgacg gtgaaggatg tgcactggat ccggcccttc
      241 tatctgtacc cgcaacaggt gacggatgag ttgctggatc tctatgccgg agaggcgcgt
      301 atcaccaagt acctggacat gccgttgcaa catatcagcg acggcatgct gaagcggatg
      361 caccggttgg gggaccgcaa acacgtcacc aagctggtcg aacgtattcg agccaagatc
      421 cccggcgtct ttttcaggac ggcgtttatc gtcggatttc ccggtgagac cgacgccatg
      481 ttcgaggagc tcaagcaatt catcctcgat atggaattcg atcgtgtggc ggtgtttctc
      541 tattctgatg aagaaggcac gtcggccgtc gatctcgacc ggaaagtcga tcaggcggtg
      601 atggaagaac gacgcaatga actgctggct ctgcaggagt cgatttcaga atccaagaat
      661 cgcgactacc tcggccgcac gatggaggtc ctggtggatg ggatctctga agaatcggac
      721 cggctgctgg aatgcagaca cgaagggttg gcgccggaga ttgacggggt ggtcttctgt
      781 gatcgcagcg cggcgaggcc gggagagttt gtttcggtca ccgttaccga tgtagccgga
      841 tatgatctaa ttgcccagcc cgttgggcgg accgatgcgc cgatctctgt tcagaccgct
      901 cccactctcc tcatgcccaa gaaatcaggg gcaggctacc gataagcctg cgctcaccag
      961 tagcctgccc gctctattct atacggacgc tcttagatct tggcgctgag gtacagggac
     1021 gcgcctctcc gattgaccag caccagcacc gtttgcccct tcttgaggtc tgatgcggcc
     1081 cgatcgaaat ccttcatcga ggtgaccggt ttgcggttga tctcccggat cacgtcgccc
     1141 ggcatcaatc cggcacgctc cgcgtcgctc tcgggatcga cggtcgtgac gacgacgcct
     1201 tgaatttttc ctttgatccc gagttcctgg gcagtctccc ggtccaactc ctgcacggcg
     1261 aggccggcca gcggctgttc cgatggaccg gcctcagcct tggcgacctg cgggttgtcc
     1321 ggcagttcag cgatggtcac ggtgaggtcc ttttcacggc catcccgcat cacctttacc
     1381 gtagccttgc tgccgaccga acttcgtgtg accgcgcgct gaagggtgac ggtgtcttcg
     1441 atcggagttc cctgaaagga
//
LOCUS       DAORQN010000321         1460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2049_length_1460_cov_2.356584, whole genome shotgun sequence.
ACCESSION   DAORQN010000321 DAORQN010000000
VERSION     DAORQN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_1460_cov_2.356584"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..390
                     /locus_tag="PK614_10235"
     CDS             160..390
                     /locus_tag="PK614_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82623.1"
                     /translation="MKVICSWCQSEGRSGLVREKAPLADLRETHGICSDHLQQMAVGR
                     VPLIPGYDEGSLPRHESVVIRRSDLAAVACYE"
     assembly_gap    775..817
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(918..1328)
                     /locus_tag="PK614_10240"
     CDS             complement(918..1328)
                     /locus_tag="PK614_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HRB82624.1"
                     /translation="MMGLLDQLGQAAAGMMGGGDKNPLMQAVVNLLGQNSSVGGLSGL
                     VQAFQKNGLGDIVNSWVSTGKNLPVSAEQIQQGLGGDLLKQLASQAGLSSEAAGGQLA
                     NLLPGLIDKLTPDGKMPDTTLIEQGLNLLRGKLG"
BASE COUNT          291 a          404 c          412 g          310 t
ORIGIN      
        1 atccacgatt cgatacagcc gattatccga atagatacac ccacgatgat tcgtagcggt
       61 atcaggcaat gttagcacag agaaacaagg agttagagac cgccgaccat atggcgcaag
      121 tcttgctgag tcttgggtat cactgcgtag gagaacacga tgaaagtaat ctgttcctgg
      181 tgccagagtg aaggccgatc ggggttggtg cgagaaaagg ctccgttggc tgatctgcgc
      241 gagacccacg gaatctgctc cgaccacctt cagcaaatgg cggtcggccg ggttccgctg
      301 atccccggat atgatgaagg ctcgctgccg cgccatgaat ctgtcgtcat ccgtcgaagc
      361 gatctggccg ccgtggcctg ttatgaatga gcaagcgtgt gcttggatgc gaccgtccca
      421 ccgtcatcgc tcttggattc cccaacgaga acgacaaaaa acgtcaatca ctgccggaat
      481 gccacgctat tgacggcctc aacggacaag cctgattggt tcacgcgaac gatcctgctg
      541 aaaacattga acacttacta gccgatgccc gctggtgcga ctcttgcgcc cccaacgtgg
      601 atggtcgcat cgcaacgaac ggagtgaagt ggtgaaagtg ctctgtgcct ggtgtgtacg
      661 agacgggaag ccggcgtttc tgcgggagaa attcccgttg gaggatccca gtgaaacgca
      721 tgggctctgt ggcgaccatt tcacctcgct cagcgcgagt gtggggaaaa tcgtnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact gacgcggtgg gcccaacgga
      841 tgatggggcg gttcttctct ctctgctagc gcggaagggc cacccggcac cctgaaatga
      901 gtgccgggaa tcgagtccta cccgagtttt cctcgcaaga gattcaatcc ctgttcaatc
      961 aatgtggtgt ccggcatctt gccgtccggg gtcagcttat cgatgagccc gggaagcaga
     1021 ttggccagtt ggcccccggc tgcttccgag ctcaatcccg cttgagatgc cagttgcttc
     1081 aggaggtcgc cgccaaggcc ctgctggatc tgctccgccg agaccgggag gttttttcct
     1141 gtgctgaccc aggaattcac gatatctccc aggccgttct tctgaaacgc ctgcacgagt
     1201 ccactcagcc cgccgacgga actgttctgc cctagcaggt tcactacggc ctgcatcagg
     1261 gggttcttat cgccgcctcc catcatgccg gctgccgcct gtccgagttg atcgagaaga
     1321 cccatcatga cctccttctg taaatagaag cggtaagccg cctagcggga tggttttttc
     1381 gcgcctttgg ccggggtgga tttcgatggt ttgcgctcca tggccaacgg cggcgcaaac
     1441 caattgtggc ggctccgttt
//
LOCUS       DAORQN010000322         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2060_length_1452_cov_4.089477, whole genome shotgun sequence.
ACCESSION   DAORQN010000322 DAORQN010000000
VERSION     DAORQN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2060_length_1452_cov_4.089477"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK614_10245"
     CDS             complement(<1..705)
                     /locus_tag="PK614_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82625.1"
                     /translation="MRTSTALMLLLLATGCSLDAASRGPFGPDDPETAVPKKPLPAQL
                     PTRHVLEFSSDESPEHIKPKMAGSALDAGVSRSPNREAVLSAADIAGLRKVAEADPRV
                     AALLGERWGFIDADRVPPRGKVSFGCCRTTARFTRLTYFSYSHNVAVHVRMKDSTILE
                     ASRAEGYLPPEGPRDIQRGIELARADRRLADKVQGMQGHGLLMQPDRGFFRNDPGYGH
                     RVIWITFSQGLGGDPKY"
     gene            complement(729..1250)
                     /locus_tag="PK614_10250"
     CDS             complement(729..1250)
                     /locus_tag="PK614_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82626.1"
                     /translation="MNRSIEGVVTFLQDNDSLIKQLMETAESATNEIEQLADRVGNAR
                     EEAQVLVDQLGAVEFSVGRQDDTKVRQAALLEAVAALRKKFETYKNDPLRRGIYAAEI
                     SNLYLQLANTFNNETVAQIVPFGADEISGYKDLMKKAVLDAASRKKRAAVIKAGVQIS
                     RLALGVAGKLALV"
     gene            complement(1262..>1452)
                     /locus_tag="PK614_10255"
     CDS             complement(1262..>1452)
                     /locus_tag="PK614_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82627.1"
                     /translation="LDQAPNRSGQIMEILDHSRTVNDALEEVVGARILRIGGLDRAQL
                     FTADLVELLDRLKKDDGQ"
BASE COUNT          251 a          433 c          445 g          323 t
ORIGIN      
        1 gtatttggga tcgccgccaa gtccctggga gaaggtaatc caaatcaccc gatgtccata
       61 cccagggtcg tttctgaaga aaccgcgatc cggctgcatc aacagcccgt gtccttgcat
      121 cccttgcacc ttgtcggcga gacggcggtc ggcgcgagcc agctcgatgc cccgttggat
      181 atcccggggg ccttcggggg gtagataccc ttccgctcgc gaggcttcca gaatggtcga
      241 atctttcatg cgcacgtgga cggcgacgtt atggctgtag ctgaagtagg tgagtctggt
      301 gaatcgcgcg gttgtccgac agcagccgaa cgacaccttc cccctcggtg gtactcggtc
      361 cgcgtcaatg aatccccatc gttcaccgag gagggccgcg actcgaggat ccgcttccgc
      421 gacctttcgc agtcccgcaa tgtcggcagc tgagagcacc gcctcccgat tgggtgagcg
      481 ggagacaccg gcatcgagtg cgcttccggc catcttgggc ttgatatgct cgggggattc
      541 atcggacgag aattccagga catgtctggt cgggagctgt gcgggcagtg gcttctttgg
      601 cacggcggtt tccggatcgt ccggaccgaa ggggccgcgt gaggcggcat cgagactgca
      661 gcctgtggcg agaaggagca gcatcaacgc ggtggatgtc cgcatgaccg gtcgagttcc
      721 catacctctc acaccaatgc cagtttgccg gccacgccaa gtgccaaacg cgaaatctgc
      781 acgcccgctt taatgacggc agcgcgtttc tttcgcgacg cggcatccag cacggccttc
      841 ttcatcaagt ctttatagcc gctgatttcg tcggcgccga aggggacgat ttgggccact
      901 gtctcgttat tgaaggtgtt ggccagttgc aggtacagat tggagatttc tgcggcatag
      961 atgcctcgtc gcagcgggtc gttcttgtag gtctcaaact tcttccgcag ggcggcgacg
     1021 gcctccagca gggctgcttg ccggaccttt gtatcgtcct ggcggccgac gctgaactcc
     1081 accgcgccca attgatcgac cagcacttgc gcttcctcgc gggcgttgcc cacgcgatcg
     1141 gccagttgtt cgatttcatt ggtggcgctt tcggcggttt ccatcagctg tttgatcaga
     1201 ctgtcattgt cttggaggaa agtgaccacc ccctcgatgc tgcggttcat agcggcctcc
     1261 gttactgtcc gtcgtctttc ttcaatcgat cgaggagttc caccaggtcg gcggtgaaca
     1321 gctgggcacg atccagtccg ccgatgcgca gaattcgcgc gccgacgact tcttccaggg
     1381 catcgttcac ggttcgcgag tgatccagga tctccatgat ctggccagac cggttggggg
     1441 cctggtcaag ga
//
LOCUS       DAORQN010000323         1432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2092_length_1432_cov_2.777052, whole genome shotgun sequence.
ACCESSION   DAORQN010000323 DAORQN010000000
VERSION     DAORQN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_1432_cov_2.777052"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK614_10260"
     CDS             complement(<1..390)
                     /locus_tag="PK614_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRB82628.1"
                     /translation="MPCRCPPNSFKDGRHWLASLYALIPLEASHKDKILARLDQTIRA
                     VVKGIVLTAIAQGLLAGAAYAVLGVPFPMVLMALTILLAPLPFGGTALIWGPVALYLL
                     WAGTVGKGLVMLAWGIGVVSTIDQILKP"
     gene            complement(619..>1432)
                     /locus_tag="PK614_10265"
     CDS             complement(619..>1432)
                     /locus_tag="PK614_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB82629.1"
                     /translation="YHYEWFPLESRSGAGKRFGLVLRDTTDESRLQDQLIQAEKSGSL
                     DVLTAGIGHELNNPLFGILGLGEAIQEEADLARAKGYAQDIVGHGRKMAAIIRDFTGV
                     AARESKSQRVPVDVTAQLEQALAIVQTSHDCLGLNVQKHYVAVSPVTALPDQLRLAFI
                     NVLTNAIQAMSGRGDLWLTTEEQDDLITITIRDNGPGISKHHLGKVFDPFYTTKGQGE
                     GSGLGLTVAQRLIKKFGGEIRLESPEGQGTTCVITLPADKAGVRKEEPCVSP"
BASE COUNT          258 a          448 c          440 g          286 t
ORIGIN      
        1 cggcttgagg atttgatcga tcgtggacac gaccccgatt ccccaggcca acatgaccag
       61 ccccttcccc accgtaccgg cccagaggag atacaaggcc acaggccccc agatgagcgc
      121 cgtcccgccg aacgggagcg gagccaggag aatcgtcagg gccatcaaca ccatgggaaa
      181 cggcacaccc agcaccgcat aggccgctcc ggcgagtagc ccctgcgcaa tggccgtcag
      241 cacgatccct ttcaccacgg cgcgaatggt ctgatccagg cgagccagga ttttgtcctt
      301 gtgcgaggct tccaggggga tcaacgcata cagcgacgcc agccagtggc ggccatcttt
      361 gaaggaattg ggcgggcagc ggcagggcat tttcgtgttc ccaatgttcc gcggcggaca
      421 cgatgccttt ggcctgcatc cggttgacga cctggttcgt gtagatggtt cgatcggcat
      481 ccagaaccgc gtgcacgaag tccgcgacct tctcgggagg aatgcccggc ggcggcgcga
      541 tatctttggc ctgcaacgaa tagctcgagg cgcaggccag cgccagtgcg agccacagcg
      601 gccgtgcccg aatgactgtc agggtgagac gcatggctcc tcctttcgaa ccccggcctt
      661 gtccgccggc agggtgatga cgcaggtcgt gccctggcct tccggactct ccagccgaat
      721 ctcgccgccg aatttcttga tcagccgttg cgcgacggtg aggccgagtc ccgagccttc
      781 tccctgtccc ttggtggtgt agaagggatc gaagactttg ccgagatggt gtttggaaat
      841 gccgggaccg ttgtcccgta tcgtgatcgt aatcaggtcg tcctgttctt ctgtggtcag
      901 ccacaggtcg ccccgcccgc tcatggcctg aatggcattg gtgaggacgt tgataaatgc
      961 caggcgaagc tggtcgggca gcgccgtcac cggcgacacc gccacatagt gtttctggac
     1021 gttcaggccg aggcagtcgt gcgaggtctg cacaatagcc agtgcctgtt ccaactgggc
     1081 ggtcacatct accggcacac gttggctttt cgattcacgg gcggccactc cggtgaagtc
     1141 ccggatgatg gccgccatct tccggccgtg tccgacgatg tcttgcgcat aacccttggc
     1201 gcgagccagg tccgcttcct cctggatggc ttccccgagg cccagaattc caaacagcgg
     1261 gttgttgagt tcatggccga tgccggcggt cagcacatcg aggctgcctg atttttcagc
     1321 ttggatcagc tggtcctgca gtcggctttc gtcggtggta tcacgcagca cgaggccgaa
     1381 gcgtttgcct gccccgctac gactttccag cggaaaccat tcatagtggt ac
//
LOCUS       DAORQN010000324         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2097_length_1426_cov_7.342086, whole genome shotgun sequence.
ACCESSION   DAORQN010000324 DAORQN010000000
VERSION     DAORQN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2097_length_1426_cov_7.342086"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK614_10270"
     CDS             complement(<1..501)
                     /locus_tag="PK614_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82630.1"
                     /translation="MAIAENEAPESSTSDPFPRGGAILSLIAVSIGIIFLLDAVTPLG
                     VPVWVLYLIPLILTYQAPVAWIPYLTAGVCAILTLLCLALSGPMTSMPSWMPLLNRAA
                     ALGMFAVIAHLIVQYRRATERLIQTAALDAEHKALQLKESLLLKNAQDVQDLYDHAPC
                     GYHSLNS"
     gene            complement(721..>1426)
                     /locus_tag="PK614_10275"
     CDS             complement(721..>1426)
                     /locus_tag="PK614_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82631.1"
                     /translation="HYTIVTDKGFTIHKTGLPALQMFLNTRMYLYSNVYYHRTTRAID
                     IHLRDIFGDTMKLLFPSNPAKHMDEYLTLTDWSLLEDVRRWKKAGQSSLRRLHQEWAH
                     ILGRDVKWKMAYSTVLKEKGIERGMDFPSHEQFQQQIQKALPAKLQALPFRVDMAPLD
                     PRPDPKDTRGIPLLVFDPGTKGVSTEPLEEFLDLLPTRLVQFRIYALDHNQDAALSRA
                     AATVLNKTPSSMETNF"
BASE COUNT          307 a          368 c          447 g          304 t
ORIGIN      
        1 cgagttcagt gaatggtatc cgcagggcgc atggtcgtag aggtcctgta cgtcctgcgc
       61 gtttttcagc agcaggctct ctttcaattg cagggccttg tgttccgcgt ccagtgcggc
      121 cgtttgaatc aggcgttcgg ttgcccgtcg gtactggacg atcagatggg cgatgactgc
      181 aaacatgccc aatgccgcgg cgcgattcag gagaggcatc catgagggca tgctggtcat
      241 ggggccggac agggcaagac acagcaatgt gaggatggcg cacactcccg ccgtgaggta
      301 gggaatccat gcgacaggag cctggtacgt gaggatcaac gggatgaggt agagaaccca
      361 gacaggaaca ccgaggggcg tcaccgcgtc gaggaggaag atgattccga ttgagacggc
      421 aatgaggcta agaatggccc ctccacgagg gaaaggatca ctcgtgcttg actcgggcgc
      481 ctcgttctcg gctatagcca catcgctcct atgcaggagt cctgaatctc ataaagagcc
      541 agtattacaa gaggctatcg caatcggagc tactatgcac caaattacct catgcgagta
      601 gagtcaatcc gacctaggca tttacctagg tgcggtattt gcgtataaga agagcgtcgg
      661 attctcgggc gatgaccgta caggcagcgg ctggaagcct tcccggaacc aggacggggc
      721 ctagaaattc gtctccatgc tcgacggggt cttattcagc acggtggcgg cggcgcggga
      781 gagggccgcg tcctgattgt ggtcgagggc gtagatgcgg aattgcacaa ggcgggtagg
      841 gagcagatcc aaaaactctt ctaacggttc agttgagaca cccttcgtgc cgggatcgaa
      901 tacgagcagg gggatgccgc gcgtatcctt gggatcgggg cgcggatcga gcggagccat
      961 gtccacgcgg aacggcaacg cctgcaattt ggccggcaac gccttctgga tctgctgctg
     1021 aaattgttcg tgactgggga agtccatgcc tcgctcgatg cctttttcct tgagcaccgt
     1081 gctgtaggcc attttccact tcacgtcgcg ccccaggata tgcgcccatt cctgatgtaa
     1141 acgacgcagg ctgctttggc cggctttctt ccatcgtctg acgtcctcca acaacgacca
     1201 gtcggtcaag gtcaggtatt catccatgtg cttggccggg ttggacggga acaacaattt
     1261 catcgtgtcc ccgaagatgt cgcgcagatg aatgtcgatg gcgcgggtcg tgcggtgata
     1321 atacacgttc gagtacagat acattctggt gttcaaaaac atctgcaggg ccggcagccc
     1381 cgtcttatga atggtgaagc ccttgtcggt gacgatggtg tagtga
//
LOCUS       DAORQN010000325         1404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2126_length_1404_cov_6.139362, whole genome shotgun sequence.
ACCESSION   DAORQN010000325 DAORQN010000000
VERSION     DAORQN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2126_length_1404_cov_6.139362"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PK614_10280"
     CDS             complement(<1..723)
                     /locus_tag="PK614_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRB82632.1"
                     /translation="MAGREFHDGATDGLEALEPLDPEKIHSFSELLEAMRKTAFGGRR
                     LGEAYETLAAMIEDADCKVVLTLSGAMTIAKMGKIISTMIDRGMVHAVVSTGALVAHG
                     LSESVGKLHYRHDASHSDEELFQKGYNRRSYLMDDEIEREVERGNSENGPDGKPPRHP
                     PIRLHPGRPVQRHHLTPDALGFFPSNRERLNGTADLATRIGQGRAGLSHHGACKLFLL
                     GLEGLSGLLKQSRARIAGQPAHR"
     gene            complement(946..1287)
                     /locus_tag="PK614_10285"
     CDS             complement(946..1287)
                     /locus_tag="PK614_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82633.1"
                     /translation="MAGFGLHNGTEGFGIVGAAGKTPISWRDVFLLGLIAGVPTCVGT
                     FLSGQGVSPYFSICFYSLAAGSLLYVVLSLTVMSYTATRRVQVAAGMFAGISLMYVTA
                     MVLTLVSSVRS"
BASE COUNT          309 a          446 c          398 g          251 t
ORIGIN      
        1 gcgatgcgcc ggttgtccgg ctatgcgcgc tcgactctgc ttcagaaggc cgctcaggcc
       61 ctccaatcca agcaggaata gtttgcacgc accatgatgg ctgaggccgg cacgcccctg
      121 accgatgcgc gtcgcgaggt cggccgtgcc attcagacgt tctcgattgc tggggaagaa
      181 gccaagcgca tcgggggtga ggtggtgccg ctggactggt ctcccgggat ggagtcgtat
      241 tggggggtga cgcggcggtt tcccatcggg cccattctcg gaattacccc gttcaacttc
      301 ccgctcaatc tcgtcgtcca taaggtagct ccggcggttg tatcccttct ggaataactc
      361 ctcgtcgcta tgggacgcgt cgtgacggta gtgaagcttg cccaccgatt cgctcaggcc
      421 atgtgcaaca agcgcgccgg tcgagaccac ggcatgcacc atgccgcggt cgatcatggt
      481 gctgatgatc ttgcccatct tcgcaatggt catggcgccc gagagcgtca acaccacctt
      541 gcaatcggca tcctcgatca tcgcggcgag tgtttcgtac gcttcgccca gacgccggcc
      601 accgaaggcc gtcttccgca tcgcctccag cagttccgaa aaagaatgga tcttctcggg
      661 gtcgagaggc tcgagcgcct ccaagccatc cgtggcaccg tcgtgaaatt cccgtcccgc
      721 catgatgaga gcctcccgaa aaaatcagtc ttccgatcga gcaggtcatt ttatacacaa
      781 ccgagcgtcg cggggtcaat tgaagcaccg atcagtcacc cgagcatgac ccggcttccg
      841 ccgaccggac gcgccctgag cgcgctcctc gaatacctcg aaaaaatgag atgggatctg
      901 cgcgaataaa acggacgcga aggaggctgc ggatggaagg agccgtcagc tgcgaacgct
      961 gctgaccagt gttaacacca tggccgtgac atacatgaga ctgatgccgg caaacatgcc
     1021 cgctgccacc tgcacacggc gcgtcgccgt gtacgacatg accgtcagcg agagcaccac
     1081 atagagcaac gagccggccg ccagcgaata aaagcagatg gagaagtagg gagacacacc
     1141 ctgcccgctg agaaatgtcc cgacacaggt agggaccccg gcgatcaagc ccagcaggaa
     1201 gacatcacgc caggatatcg gtgtttttcc agccgcgccc acgataccga atccctcggt
     1261 accgttgtgc agaccgaaac ccgccaccaa taacgcgctg agcgtatatt caccgctgcc
     1321 ttagatggac cgcttggcct ccccaacgcc cgaattaggc ataccaaacc cccccgaatt
     1381 aatgtaggga accacaacca tggg
//
LOCUS       DAORQN010000326         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2153_length_1389_cov_10.634933, whole genome shotgun sequence.
ACCESSION   DAORQN010000326 DAORQN010000000
VERSION     DAORQN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_1389_cov_10.634933"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>909)
                     /locus_tag="PK614_10290"
     CDS             complement(<1..>909)
                     /locus_tag="PK614_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB82634.1"
                     /translation="QQVRRSTETSDKKLAEAILSKIRVQIVEGKYFEKPKEDPYTFSQ
                     LMDRYLKEHASRRAHYRRYVNMVANVKTFFGNPNLSQITPKTIVAFKAKRYADGVMPA
                     TINRELAMLKKAFNLACREWEWAKDNPVCRVSMEKEQNSRDRWLTEQEEARLLMAAAA
                     WLGEVVTFAVHTGMRRGEILALTWAGVDFTRRTVTVFKSKNGERRTIPVNQTVLELLS
                     QKYEQGRGLRGIETRVVFCSEAGTELDGSNLRRGFTSALKAAQIEDLHFHDLRHTFAT
                     RLVQAGVDLYKVQRLLGHKSPSMTQRY"
     assembly_gap    910..936
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(960..1268)
                     /locus_tag="PK614_10295"
     CDS             complement(960..1268)
                     /locus_tag="PK614_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB82635.1"
                     /translation="MLTVKELSAWLNIKQSTLYLWVSQNKIPCHRIHGLIRFDPDAIQ
                     TWLKGFGANPSNRFPLPTHQDTCDLDQLIEAAKSEVYTRGGETITPSPHTKEEQHGAR
                     "
BASE COUNT          266 a          374 c          382 g          340 t
ORIGIN      
        1 ataccgttgg gtcatgctcg gagatttgtg cccgagcagc cgctggactt tgtataagtc
       61 cactccggct tggactagcc gtgtagcaaa ggtatggcgc agatcgtgga aatgaagatc
      121 ctcaatctgt gccgccttca gagctgaggt aaatcctcgt cgaagattgc tgccgtccaa
      181 ctcggtaccg gcttcgctgc agaacacaac ccgggtttca attccccgta gcccacggcc
      241 ctgctcatat ttctgagaga gcagttccag tacggtttgg ttgacgggaa tagtccgccg
      301 ctctccgttc ttggatttaa acaccgtgac ggttcgtcgg gtgaagtcta ccccggccca
      361 tgtgagggca agaatctctc cacgccgcat gccggtatgg acggcgaagg tgacgacttc
      421 ccccagccag gcggctgcag ccatgagtag ccgtgcttcc tcctgttcgg taagccagcg
      481 gtcccgtgag ttctgttcct tctccatcga cacccgacag accggattgt cctttgccca
      541 ttcccattct cgacaggcca ggttgaaggc ctttttcagc atggccagtt ctcggttaat
      601 ggtggccggc atgacaccat ctgcatagcg cttcgctttg aaagccacga tggtcttcgg
      661 ggtaatctgc gaaaggttcg gattcccgaa gaatgtcttc acattcgcca ccatgttcac
      721 atagcgccga tagtgggcgc ggcgactcgc atgctctttc agataccggt ccattaactg
      781 ggaaaaggtg tagggatctt ctttcggctt ttcgaagtat ttcccttcga caatctgtac
      841 tcggatctta ctcagaatgg cttcggccaa ctttttgtcc gaggtttccg tcgatcgccg
      901 tacttgctgn nnnnnnnnnn nnnnnnnnnn nnnnnnacat ccaccaggta ttgttccgtt
      961 taacgagccc catgctgctc ctccttcgtg tgtgggctcg gtgtgatggt ttccccgccg
     1021 cgagtataga cctcggattt cgccgcttca atgagctggt caaggtcgca agtgtcttgg
     1081 tgagtaggca gtgggaaccg attgctggga ttggccccaa agcccttcag ccacgtctgg
     1141 atggcgtcag gatcgaaccg gatgaggcca tgaatgcgat ggcaaggaat cttgttctgt
     1201 gagacccaga gatagagcgt ggattgtttg atgttgagcc aagccgagag ttctttgaca
     1261 gtgagcatga tgcagataca ccgggggagt ctcgcgacac ccccagtccc cctaacatgg
     1321 gctgcccgcg tgactgccgg cagaccggtg aaaccgattg ggtggttcct tgagcaaatt
     1381 gcggtgtcg
//
LOCUS       DAORQN010000327         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2159_length_1385_cov_6.595489, whole genome shotgun sequence.
ACCESSION   DAORQN010000327 DAORQN010000000
VERSION     DAORQN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_1385_cov_6.595489"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PK614_10300"
     CDS             complement(<1..1108)
                     /locus_tag="PK614_10300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB82636.1"
                     /translation="MMHLSWQVVVTLSVSAVLVHTAGNLAEARSERLTTPNNSPLLLA
                     QATGAAETSDSVDFNILPQALGSAITAFGDQANYRLLLPSDLAEGRSTSGVSGRHTPE
                     EALALMLAGTGLSYRMTEARTMTLEPVAATPLPLAPLSQEPAPPNPNTQIQAAPKPVK
                     VPEIVVKEVEDRGYAVDESSSATRIPAPIHDTPRSVEVVTRQVLDDQKVIRFSEALRN
                     VSGVSQSSTQGGQGGTFMIRGFASDLNVFKNGFRDDSTFSARAQRDIINIESVEVVKG
                     PPSYLYGRSDPGGVINQVTKAPLRNPYYSAEMIVGSYGLYRPTIDIGGPLNESKTLTY
                     RFNGMYESAESYRDGVKTERIFLAPTFGWEMSSRT"
BASE COUNT          280 a          363 c          439 g          303 t
ORIGIN      
        1 tcgtccgtga actcatttcc caaccgaagg tcggggcgag gaagatacgt tcggtcttca
       61 cgccatcccg ataactctcc gccgattcat acatgccgtt gaagcggtag gtgagcgtct
      121 tgctctcgtt caggggacca ccgatgtcga tcgtggggcg gtacagtccg taactgccga
      181 cgatcatctc tgccgaatag tagggatttc tcaacggagc ctttgtgacc tggttgatga
      241 ctccgccggg atcagagcgg ccgtacaggt aggatggcgg acctttcacg acttcgacgc
      301 tctcgatgtt gatgatgtcg cgttgcgcac gggcactgaa ggtactgtcg tcccggaagc
      361 cgtttttgaa gacgttcagg tcggaggcga agccccgaat catgaaggta ccgccttgtc
      421 cgccttgcgt gctggactgc gagacgccgc tgacgttccg gagggcttca ctgaaacgaa
      481 tcactttttg atcgtccagc acctgtcggg tcacgacctc cacggagcgt ggtgtgtcat
      541 ggatcggagc gggaatacgc gtggccgaag aggattcgtc gacggcatac ccgcgatctt
      601 cgacttcctt cacgacgatt tccggcactt tcaccggctt gggtgccgct tgaatctggg
      661 tgttggggtt gggcggcgct ggctcttggc tcagcggagc gagaggcaag ggcgttgccg
      721 ccacgggttc caacgtcatc gttcgggcct cggtcattcg atagctaagg ccggttccgg
      781 cgagcatcag cgccaaggcc tcctccggag tatgacgacc gctgacgccg ctggtgctcc
      841 ttccttcagc cagatcggat ggcagcaaga gacgatagtt ggcttgatcg ccaaacgcgg
      901 tgatggcgga ccccaaggcc tgcggtagaa tattgaagtc caccgagtct gatgtctcag
      961 cagcccctgt ggcctgcgca aggagaagtg gactgttgtt cggcgtggtg agacgctcgc
     1021 ttcgcgcttc agccaggttt ccggccgtat gcaccagaac ggccgatacg gagagtgtga
     1081 cgacgacttg ccatgagaga tgcatcattc cccctctatt agtccggtat gaataagcgc
     1141 acgtatccgg gcacgggccg agcgacgcgc actcggcggt tcattaggta aagacggacc
     1201 gggccgaaaa acttgtaatc gaaatcgtgt gcgttgaaaa aaagcttgaa ggagatgatg
     1261 gaagacaggg tctaattgtg aagaggatgg atgacgtctc aggaaaactg cggtgtctgt
     1321 tagtgcaaga ggacgaggta gggcgtcagg gtggtgtcgt gaattcgcaa cgtctgttca
     1381 atcat
//
LOCUS       DAORQN010000328         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2160_length_1385_cov_5.663158, whole genome shotgun sequence.
ACCESSION   DAORQN010000328 DAORQN010000000
VERSION     DAORQN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2160_length_1385_cov_5.663158"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PK614_10305"
     CDS             <1..1225
                     /locus_tag="PK614_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HRB82637.1"
                     /translation="IGKQRWCDPRYWNMSKQAVALEALPLLGRHTAAVIAADLGLSRK
                     CLVLDLDNTLWGGVIAEDGLAGIKLGQGPDGEAFVAFQEYLLKLKRKGVILTVCSKNN
                     HADAVQPFEKHPEMRLKLQDIALFLANWESKPDNIRTIAKTLQIGLDALVFVDDNPVE
                     REIVRKFLPEVDVIPLPEDPSYYLRTLSDYLLLETSSLTAEDTERTDQYRARAQIMEL
                     EAAAGSIEDFYRSLRMQAIVVPFDASQLPRIAQLIGKTNQFNLTTRRHGMAQLEAFVL
                     DSSYVHLALRLRDRYTDHGLVSVMIARQQGDILDIDTWLMSCRVIGRTVETTMLEQLC
                     RRAVQLGCTSLRGTYIPTQKNAMAADAYAKHGFERVGNHEGFEVWIYDLAAKGLIANT
                     FVKPVDSWELADGAC"
BASE COUNT          304 a          402 c          398 g          281 t
ORIGIN      
        1 cattggaaag cagcgatggt gcgatccgcg ctattggaat atgtcgaaac aagcagtcgc
       61 actggaggcc ctgcctctgc tcggaagaca tacggcggct gtgattgctg cggatctcgg
      121 gctcagccgt aaatgtctgg tgctggattt ggacaatacg ttatggggcg gtgtcattgc
      181 ggaagacgga ttggcgggca tcaagttagg gcaagggccg gacggcgagg cgtttgtggc
      241 gtttcaggag tatctgctga agctcaaacg caaaggcgtg atcctgacgg tctgctcgaa
      301 gaataaccac gccgatgccg tacaaccctt cgaaaaacat ccggagatgc gactgaagct
      361 gcaggacatc gccctcttcc ttgccaattg ggagtccaag cccgacaaca tccgcaccat
      421 cgccaagacg ctgcaaatcg gtttggatgc gttggtcttc gtcgatgata accctgtcga
      481 gcgcgagatt gtgcgcaagt tcttacccga agtcgatgtc atcccgctgc cggaagaccc
      541 ctcctattac ctcagaactc tgtcggacta tctcttgctc gagacaagct cattgaccgc
      601 cgaagacacg gagcggaccg accaatatcg ggccagagcg cagatcatgg agttggaagc
      661 ggctgccggt tccattgaag atttttaccg cagccttcgg atgcaggcca tcgtggtgcc
      721 gttcgatgca tcacagttgc cgcgcattgc ccaactcatc ggcaaaacca accaattcaa
      781 tctcaccact cgacgtcacg gcatggcgca actggaagcc ttcgtcctgg actcgagtta
      841 tgtccatttg gcactgcggt tgcgtgatcg ctacaccgac catggactgg tcagcgtgat
      901 gatcgcgcga caacagggag acatcctcga tatcgacacg tggctcatga gctgccgggt
      961 catcggccgg accgtggaga cgacgatgct ggaacagctc tgtcggcgcg ccgtgcagct
     1021 tggatgcacc tcgcttcgag gcacctacat tccaacgcaa aagaatgcga tggcggcgga
     1081 cgcctatgcc aagcatggct tcgagcgagt cgggaaccat gaaggattcg aagtctggat
     1141 ctatgacctg gcggccaaag gcctgattgc caatacgttc gtgaaacctg tcgattcctg
     1201 ggagctcgcg gatggcgcct gctgacgcgc catccggggc tcccccctcc ctgccacatg
     1261 tgaccacagt ctgtttcacg acgcaggatc tcgcccagtt cagcgcagcc agcggggata
     1321 gaaaccccct gcatctctcc gaagaatatg cgcgagccac accctacggc gagcccgtgg
     1381 tattc
//
LOCUS       DAORQN010000329         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2198_length_1353_cov_3.497689, whole genome shotgun sequence.
ACCESSION   DAORQN010000329 DAORQN010000000
VERSION     DAORQN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2198_length_1353_cov_3.497689"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>215
                     /locus_tag="PK614_10310"
     CDS             2..>215
                     /locus_tag="PK614_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82638.1"
                     /translation="MRATVGVLLIVGALSASACHRHHTPAERADWMTGKIAKHLDLDD
                     SQKTALAAVKDEALAARAESQKEHQAL"
     assembly_gap    216..225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <226..431
                     /locus_tag="PK614_10315"
     CDS             <226..431
                     /locus_tag="PK614_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRB82639.1"
                     /translation="LIAQVQGDRLDQTKVAQLIERHHALQSRMMTRVLPKAAEWHAGL
                     RPEQKAEAVEHIRRWIAHYEGGR"
     gene            446..799
                     /locus_tag="PK614_10320"
     CDS             446..799
                     /locus_tag="PK614_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82640.1"
                     /translation="MAGYRLGLTEESKRATIMLKIMIAQDAQGTAIVLAGRLVGPWVE
                     EVRRCWLEAEAECRDKWRVNLRETTHVDAAGKALLSEMFHRGATFEATGCLTRAIVES
                     LAVASAPASKGGLES"
     gene            893..1231
                     /locus_tag="PK614_10325"
     CDS             893..1231
                     /locus_tag="PK614_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82641.1"
                     /translation="MRQVIWSCVSLFVVSLVLAVSPVLADVDDGSHIKITSPKDGATV
                     GDTFELTYELTKGSKAAHGHVYLDGEPQKKFPGTFKGLSKGKHEIKVQAASHDHDLLA
                     ATDTITVEVQ"
BASE COUNT          302 a          345 c          443 g          253 t
ORIGIN      
        1 catcagagcg acggtcgggg ttctgctgat cgtgggagca ttatctgcgt cggcctgtca
       61 ccgccaccat actccggccg aacgggccga ctggatgacg ggtaagatcg ccaaacatct
      121 ggatctcgac gacagccaga aaacggctct ggccgccgtg aaggatgagg ccttggccgc
      181 gcgcgcggag tcgcagaagg agcaccaggc gctggnnnnn nnnnnaactc atcgcccagg
      241 tgcaagggga tcggctcgat caaacgaagg tggctcagct catcgagagg catcacgcgc
      301 tgcagagccg gatgatgacg cgagtgctgc ccaaggcggc tgagtggcat gccggtctgc
      361 ggcctgagca aaaggctgaa gcagtggagc atattcggcg gtggatagcg cattacgaag
      421 gcggtcgata atctctcagt gttgtatggc cgggtaccga ctcggcctga cggaagagtc
      481 gaagagggcc accatcatgc tgaagatcat gattgcacag gatgcgcaag gcacggcgat
      541 cgtcctcgcg gggcggttgg tcggtccctg ggtagaagaa gtgcggcgct gctggcttga
      601 agcagaggcc gagtgcaggg acaagtggcg ggtcaatctt cgcgagacca cacatgtgga
      661 cgcggcgggc aaggccctgc tgtcagagat gtttcaccgg ggggcgacgt tcgaggccac
      721 aggatgtctg acgcgcgcca tcgtggagag tctggccgtc gcatccgccc cggcttctaa
      781 gggcgggctg gaatcatgat gtggaaccaa agcagttgac agcagtgagt cgtttagaaa
      841 cattcacttc tccggatcac cattgatccg gaatttcacc aaggagagca ccatgcgaca
      901 agtgatatgg tcttgtgttt ccctgttcgt cgttagtctg gttcttgccg tcagccctgt
      961 gttggccgac gtcgatgacg gatcacacat caagatcacc tccccgaagg atggggcgac
     1021 ggtgggagac accttcgagc tcacctacga actcaccaag ggttccaagg cggctcatgg
     1081 gcatgtgtac ctggacggcg aaccgcagaa gaagtttccc ggcacgttca aaggcttgag
     1141 caagggcaag catgaaatca aagtgcaagc ggcgtcccat gatcatgacc tccttgccgc
     1201 gaccgatacg atcacggtgg aggtgcaata gggcgagatc acgggtagtc gtcagatttg
     1261 aagatcaccg tggggaggga catatggccg taaccttaat caatgtgttt tcagtaccga
     1321 agggtaaaga agatgagttc gtgaagtggt ggc
//
LOCUS       DAORQN010000330         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2249_length_1320_cov_5.610277, whole genome shotgun sequence.
ACCESSION   DAORQN010000330 DAORQN010000000
VERSION     DAORQN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_1320_cov_5.610277"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PK614_10330"
     CDS             complement(<1..680)
                     /locus_tag="PK614_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRB82642.1"
                     /translation="MGRERSQGKRAQSAPPAGTLFIVGVPIGHPDDLTIRGLATLRQV
                     DLIAAKNPQATHALLAHHGIHTTITTYDRNNAADKVPLLLERLRQGCRVALVSDCGMP
                     VVYDPGRLLITAASRAQIPVKVIPGTSAVVAATALAGMDGNAFVFDGRWTGGTKALTG
                     RLQSLQSEPRTMIFFPPAQSLRQFLTLLRRTLGNRKVVMAIDMTQSTEQIIRGRVQQL
                     LSNKPSPE"
     gene            745..>966
                     /locus_tag="PK614_10335"
     CDS             745..>966
                     /locus_tag="PK614_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82643.1"
                     /translation="MSSVFFMLTMGLYFVSTVCYLAYLLRRAETLSKVSLGITAVGFA
                     THTVALVARMTDTAQAQLPTFHEALSFFSW"
     assembly_gap    967..989
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1038..>1320
                     /gene="ccsA"
                     /locus_tag="PK614_10340"
     CDS             1038..>1320
                     /gene="ccsA"
                     /locus_tag="PK614_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRB82644.1"
                     /translation="MSLVTAAAFRSDEASSLQPVFKTLWVHVTLSMLGTVGFAVAFVA
                     GVMYLIQDGLLKSKRFNVLYAKLPALDYLDHLNQQSIVMGFPLLTLGIIS"
BASE COUNT          232 a          366 c          381 g          318 t
ORIGIN      
        1 ttttcggggc tcggcttgtt cgagagcagt tgctgaactc gtcctcgtat gatctgctcc
       61 gtactctggg tcatgtcgat ggccatcacg actttccggt tgcctaacgt ccgtcgtagc
      121 agagtgagaa attgtcggag gctctgtgcc ggcgggaaga aaatcatcgt gcgtggctcg
      181 gattgtaatg attgaagacg gcccgtgagt gctttagtgc caccggtcca acgcccgtca
      241 aagacgaagg cattgccgtc cattccggcg agggctgtgg ccgccaccac agcggaagtt
      301 cccgggatga ccttgacggg aatctgcgcc ctcgatgcgg ctgtgatgag caatcgaccc
      361 ggatcgtaga caaccggcat tccgcaatcc gacaccagcg ccactcgaca tccctgtcgc
      421 aagcgttcca acaatagtgg aactttgtcg gcggcattgt tccgatcata ggtcgtgatc
      481 gtggtgtgga ttccgtggtg ggccaggagg gcgtgcgtgg cctgcggatt tttagcggca
      541 atgagatcaa cctgccgaag tgtcgcgaga cctcggatcg tcagatcgtc aggatgtccg
      601 atcggcacgc cgacgatgaa taacgttcct gccggggggg cagattgcgc gcgctttcct
      661 tgactccgtt ctcgtcccat cgatataatt tccagttacc agaaaaagtg gctttgcgct
      721 gaaatgttgc gaggatagag cacgatgtct tcggtgtttt tcatgctcac gatgggcctg
      781 tatttcgtga gcacggtttg ctatctggcc tatctgctcc ggcgggcgga aaccctgtca
      841 aaggtgtccc tcggaatcac ggctgtcggg tttgccaccc acacggtggc cttggtcgcc
      901 cggatgacgg acacggccca ggcgcaacta ccgacatttc acgaagccct gtcgtttttc
      961 tcctggnnnn nnnnnnnnnn nnnnnnnnnc gccgccagtt gcatgtcctt ggttccttca
     1021 tcgtaccact ggcgctcgtg tcgttggtca ccgctgcggc gttccgctcc gacgaggcgt
     1081 ccagtcttca gccggtcttc aaaacgttgt gggttcacgt caccctcagc atgttgggca
     1141 cggtcggctt tgccgtcgca ttcgtcgccg gggtgatgta tctcattcag gatggtcttc
     1201 tcaagtcgaa gcgcttcaac gtgctctacg caaaactgcc cgcgctggat tatctcgatc
     1261 acttgaatca gcagtcaatt gtgatggggt ttccgctcct gacgctcggg atcatcagcg
//
LOCUS       DAORQN010000331         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2320_length_1283_cov_4.062704, whole genome shotgun sequence.
ACCESSION   DAORQN010000331 DAORQN010000000
VERSION     DAORQN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_1283_cov_4.062704"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK614_10345"
     CDS             complement(<1..329)
                     /locus_tag="PK614_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82645.1"
                     /translation="MLRTLSLLILLGLLVVEAGCSTPSGPRLTGAVRYFNIRSEVAPR
                     HLIVQVGDEIRWQNLNSQAVRLRMLEETNPELISCDKGFSQFGVLQDSVTIAPMQSVS
                     LCFSKPTT"
     gene            471..>1283
                     /locus_tag="PK614_10350"
     CDS             471..>1283
                     /locus_tag="PK614_10350"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRB82646.1"
                     /translation="MSLTFDEARHFLSRTAFGGTPEEIGHVMQLDRAAAVAQALAIPT
                     NKAQTTPPAWIHRLPPLPKVRKHWSEAQRKTFREGLKADGQELKAWWYRELHTSRCPL
                     LERMTLWWHNHFTSSLHKVKWPPFLYQQNVLLRLYALGSFRTLLTAIARNPAMLLYLD
                     TQSNRKEHPNENFARELFELFTLGEGHYTEQDIKEAARAFTGWHLDFHTGAFRVDLRH
                     HDDGRKVVFGKSAPFEGDDILLLTLEQPQVARYIVGKLWREFISDRPDSLEVD"
BASE COUNT          276 a          347 c          392 g          268 t
ORIGIN      
        1 gtggtgggtt tcgagaagca gaggctgacg gattgcatgg gagcgatggt caccgagtcc
       61 tgtaggactc caaattggga aaaccctttg tcacaggaga tcaactcggg gttggtttct
      121 tcgagcatcc gtaaccggac tgcctgggaa ttcaggttct gccatcggat ttcatcgccg
      181 acctgcacga tcaaatgacg cggcgcgacc tccgagcgga tattgaaata gcggaccgca
      241 ccggtgaggc gcgggccgga tggggtgctg caaccggcct ctacgacgag caggccgagt
      301 aaaattagaa gcgacagcgt tcgaagcatg cgcgatacta cagaactatg ccgcgcattg
      361 gctactagga ttgttaccag gtagggcgac gagaagaaac cctgtcaacc tggttgcgac
      421 agagtcgttg tcagagagag cgccgcgttc actgttccaa gagttcacgc atgagtctga
      481 ccttcgacga ggcccgccac tttctctccc gcacggcgtt cggtggaacg cctgaagaaa
      541 tcgggcatgt gatgcagctg gatcgtgccg ccgcggtggc gcaggcgttg gcgattccca
      601 cgaacaaggc tcagacaacg ccgcctgcct ggatccatcg tctccctccg ttgccgaagg
      661 tgcgtaagca ctggagcgag gcgcagagga agacgttccg cgaaggactg aaggcggatg
      721 ggcaggaact caaggcttgg tggtatcggg aactccacac ttcgcggtgt ccgctgctgg
      781 agcgcatgac gttatggtgg cacaaccact ttacgtccag tttgcacaag gtgaagtggc
      841 cgccgttttt gtatcagcag aatgtcctgc tgcggctcta tgctctgggg tcgtttcgca
      901 cgctgctcac cgccatcgcc aggaatccgg cgatgcttct gtatctcgac acacagagca
      961 accgcaaaga acatcccaat gaaaattttg cccgtgagtt gttcgagctc ttcacgttgg
     1021 gagaaggcca ctatactgaa caggacatca aggaggctgc acgggcattt accgggtggc
     1081 atctcgattt ccataccggc gcgttccgtg tcgatctgcg tcatcatgac gacggacgca
     1141 aagtcgtatt cggaaagtcc gctcccttcg agggcgacga tattctcttg ctgacgctgg
     1201 agcagcctca ggtggcccgg tacatcgtcg ggaagctctg gcgtgaattt atttcagacc
     1261 ggcccgactc gctggaagtg gat
//
LOCUS       DAORQN010000332         1264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2347_length_1264_cov_3.440033, whole genome shotgun sequence.
ACCESSION   DAORQN010000332 DAORQN010000000
VERSION     DAORQN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2347_length_1264_cov_3.440033"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..550)
                     /locus_tag="PK614_10355"
     CDS             complement(425..550)
                     /locus_tag="PK614_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82647.1"
                     /translation="MRRVHTCPSILPYEVIPFQRKMAFYNIASMDSPDSERIMVT"
     gene            complement(605..958)
                     /locus_tag="PK614_10360"
     CDS             complement(605..958)
                     /locus_tag="PK614_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82648.1"
                     /translation="MHVMTRIGFVGLGIALVGALSGTPVFAADKPVEDGSKIVITAPK
                     DRDTVGDSFELKYDLTKGSQAAHAHVYLDDQYQKGFGGTFKGLPKGKHKVTVTGATKP
                     HARPTATQSINVEVQ"
     gene            1080..>1264
                     /locus_tag="PK614_10365"
     CDS             1080..>1264
                     /locus_tag="PK614_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRB82649.1"
                     /translation="MATHYDIIIIGTGPGGGTLAYKLAPSGKKILLLERGGYLPREKD
                     NWSSKRVFIDNKYKAKET"
BASE COUNT          273 a          351 c          341 g          299 t
ORIGIN      
        1 ggtctgcgta gagtcgcatt acagcaatcg cagtgaggcg aagaagtttt cgatcaacaa
       61 atagcttgaa cgatgcgtcg acagggagac tatcgcgggc tggccgatcc cagagaaact
      121 cgatgcggtc ttctctatca cctccaaaca atctgaaaga actcaagagg tttctgacta
      181 tgggttccgt tgcattcatc ggtagcaggc ataaagccta cataccgttc attgacgagt
      241 caacgccgga cctcggtgag atttgcagac ctcatgtcat ttttccatcg acgacgattg
      301 atgcctcact tagcctttcg gattaacctt ctggatctgg cctccggttg atccaaccgc
      361 gcgcatccaa cgtccccttt tttattgcgg gggctatgaa cacacatggc accgaccagt
      421 attattaggt gaccataatt cgctcggagt ccggtgagtc catgctggcg atgttgtaga
      481 aggccatctt tcgctgaaac ggaatgactt cgtacggaag aatggagggg cacgtgtgga
      541 cccggcgcac cggagcctcc cttgcaagag gctccggcgg cggtgaagaa agttcgtcgc
      601 agcgctactg tacttcgacg ttgatgctct gcgtggcggt ggggcgggcg tggggctttg
      661 tggcgccggt cacggtgacc ttgtgtttgc ctttgggcag gcccttgaac gttcccccga
      721 agcccttctg atactgatcg tccaggtata cgtgggcatg tgcggcttgc gagcccttag
      781 tcaaatcata cttcagctca aagctgtcgc cgactgtatc ccggtccttc ggggccgtga
      841 tcacgatctt cgacccgtcc tctacaggct tatctgcggc aaagaccggc gttccgctga
      901 gtgcacccac taacgcaatg cccagtccca caaatccgat acgtgtcatc acatgcataa
      961 cggcctcctt gtttaaacca gtgaattatc acgacaccct atcactccct acggatcaat
     1021 tgctcggttg gattctttcc cctgctgtaa gattctgccg gttccgccga ctatctgcca
     1081 tggcgactca ttacgacatc atcattatcg gcaccggtcc tggcgggggc acgctggcct
     1141 ataagctggc gccgtcgggg aagaagattc tcctgttgga gcggggtgga tacctgccgc
     1201 gagagaaaga caattggagc tccaagcggg tcttcatcga caacaagtac aaggccaagg
     1261 aaac
//
LOCUS       DAORQN010000333         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2364_length_1252_cov_4.640769, whole genome shotgun sequence.
ACCESSION   DAORQN010000333 DAORQN010000000
VERSION     DAORQN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_1252_cov_4.640769"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..769
                     /locus_tag="PK614_10370"
     CDS             17..769
                     /locus_tag="PK614_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRB82650.1"
                     /translation="MSAPPSDRKQPASAALVIFAKAPIPGQVKTRLCPGLTEDEAATL
                     HGSFVLDTLERTKAAVHKFKLPVDRFLACAPSSTHVFFRIMEARHGVTLLDQPGDNLG
                     DRMAQAFSTLFARGYRHVFLVGTDVPSLPLTHYRDAVQSLTRHDLILGPAKDGGYYLI
                     GMTAPHPDLFSDIPWSTDQVMGLTQQKAAAAGLKTALLPAWSDVDTLDDLHALIDDCA
                     ADKKRPKQERVYSQRTAGTLELLAKRLRTRHT"
     gene            780..>1252
                     /locus_tag="PK614_10375"
     CDS             780..>1252
                     /locus_tag="PK614_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB82651.1"
                     /translation="MPNKVALITGGAKGIGRAIALDLAAQQWSVAICYRTSNAAADET
                     SAAIRARGGQALAVQCDVSDPKAAGDLVSQVESRWGRIDALINGAGPYHRVNLFDETP
                     AGWREMFEGNLHPIFYLGQAVAPGMKARKQGRIINFSMANADQMVAQPEVTGHYIA"
BASE COUNT          263 a          424 c          363 g          202 t
ORIGIN      
        1 attgtgagat aaacccatga gtgctccccc ctcggaccgc aaacagcccg catcggccgc
       61 cctggtgatc ttcgccaaag ccccgatccc gggacaggtc aaaacacgcc tctgccccgg
      121 cctgaccgaa gacgaagccg ccacactgca cggcagcttc gtcctcgaca ccctcgaacg
      181 cacgaaggcg gcggtgcata agttcaagct tcctgtcgat cgctttctgg cctgtgcgcc
      241 ctcaagcacc catgtctttt tcagaatcat ggaagcgcgc cacggcgtca cgctcctcga
      301 tcaaccaggc gacaacctgg gggacaggat ggcgcaggcg ttcagcacgc tctttgcccg
      361 tgggtaccgg cacgttttcc tggtgggaac cgacgtaccc tctctgcccc tgacccatta
      421 tcgcgatgcc gtgcagtcac tcacccggca cgatctgatc ctcggtccgg caaaagacgg
      481 cggctactat ctgatcggca tgacggcgcc acaccccgac ctgttctcgg acattccctg
      541 gtcgacggac caggtcatgg gactcacgca gcagaaagct gcggccgcgg gattgaagac
      601 cgccctcctg ccggcctgga gcgacgtgga caccctcgac gatcttcacg ccttgatcga
      661 tgattgcgca gcggacaaaa aacgccccaa acaggaacga gtgtattcgc aacgaacggc
      721 cggcacgctg gagttgctcg cgaaacgcct gcgcacgcgc cacacgtaac ggagtcccta
      781 tgccgaataa agttgccctc atcaccggcg gcgccaaggg aatcggccgc gccatcgcgc
      841 tcgatctcgc cgcgcaacaa tggtccgtgg cgatctgcta ccgcaccagc aacgcagccg
      901 cggatgaaac cagcgcggcc atcagggcac gcggcgggca agccctggcc gtgcagtgcg
      961 acgtctcaga cccgaaggcc gccggcgact tggtctcaca ggtcgaaagc cggtggggaa
     1021 gaatcgacgc actgatcaac ggcgccggcc cctatcaccg cgtcaatctc ttcgacgaga
     1081 cgccggcggg ctggcgtgaa atgttcgagg gaaacttaca cccgattttt tatcttgggc
     1141 aggcggtcgc acccgggatg aaggcacgga agcagggacg catcatcaac ttcagcatgg
     1201 ccaacgcgga ccagatggta gcccagccgg aggtgacggg gcactatatc gc
//
LOCUS       DAORQN010000334         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2381_length_1242_cov_8.315922, whole genome shotgun sequence.
ACCESSION   DAORQN010000334 DAORQN010000000
VERSION     DAORQN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2381_length_1242_cov_8.315922"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1242)
                     /locus_tag="PK614_10380"
     CDS             complement(<1..>1242)
                     /locus_tag="PK614_10380"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HRB82652.1"
                     /translation="RLRLLSSIVVMMLACAVGERGVWAGDDLAPLLTRGPLRVSNDNP
                     RYFADSENRIVYLTGSHSWENFQDITDAFDYQAYLDRLAALNHNFIRLWVSESIQSDG
                     GWLMHPPARENLVVPLPYLRTGPGVALDGGPRLDLSQLNPAYFERLRARVQQAGQRGV
                     YVSVMLFQGFSVVERGAWKAHPFNGNNNVNGIDGDEDGDGVGTEVHRSPTSQVLNVQE
                     AYVRKVLDTLHDLDNVLYEVVNEAIPESVPWQYHVIRFIKQYERDRQYAAHPVGMTFF
                     QLGEFGGGDNATLFDSPVDWISPGGYIKYARNPPVADGRKVMLLDTDHIHGMGGDRDF
                     VWESFVLGYNSIYMDPFGTLHELTIGEPVLNEPYCAERRTGASSFIQTARSWSTVIMV
                     IERRYFSRSKNSSAGKTDRSY"
BASE COUNT          290 a          351 c          342 g          259 t
ORIGIN      
        1 taatatgatc gatcggtttt cccagcacta ctgttcttgg aacgggaaaa ataacgtctt
       61 tcaataacca taatcacggt tgaccatgaa cgggctgttt gaataaacga agatgcgcca
      121 gttcgccgct cggcacaata cggttcattt aagaccggct ccccgatcgt cagttcatga
      181 agagtgccga atgggtccat gtatatggag ttgtagccta gaacgaagct ttcccagaca
      241 aagtcccggt cgccgcccat cccgtgaatg tgatcggtat cgaggagcat gaccttgcgg
      301 ccgtcggcca cgggaggatt tcgcgcatac ttgatataac cgcccgggga gatccaatcg
      361 accggactgt cgaacaaggt ggcattgtcg ccaccgccga actcaccgag ttggaagaag
      421 gtcatcccga cgggatgggc tgcgtactga cggtcacgtt cgtactgttt gatgaagcga
      481 ataacatgat attgccaggg caccgactcc ggaatggctt cgttgacgac ttcgtataac
      541 acattgtcca ggtcgtgcag tgtgtccagc accttccgca cgtaagcctc ttgcacgttc
      601 aacacctggg aggtaggcga ccgatgcacc tctgtgccga cgccatcccc atcttcgtcc
      661 ccgtcgatgc cgttcacatt attgttgccg ttgaacggat gcgccttcca cgcgccgcgc
      721 tcgaccacac tgaagccctg aaagagcatt acggagacgt agaccccacg ctgaccggct
      781 tgctggaccc tggccctgag ccgttcaaaa taggctggat tcaattggga caaatccagg
      841 cgaggccccc cgtccaatgc cactccagga ccggtgcgca aataaggaag cgggacgacc
      901 aggttttccc gggcgggagg atgcatgagc caccctccgt cggattggat ggattcgctg
      961 acccacaatc gaatgaagtt atgattcaat gctgccagac gatcgagata ggcctgatag
     1021 tcgaaggcgt cggtgatgtc ctgaaagttt tcccaggaat gggatcccgt caggtagacg
     1081 atccgattct cggagtccgc aaagtatcgg ggattgtcgt tggacactcg cagaggcccg
     1141 cgcgtcagga gtggcgcgag atcatcccct gcccagactc cgcgttcacc gactgcacag
     1201 gccaacatca tgacgacgat agaactcagc agacgcaatc gg
//
LOCUS       DAORQN010000335         1230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2400_length_1230_cov_5.129362, whole genome shotgun sequence.
ACCESSION   DAORQN010000335 DAORQN010000000
VERSION     DAORQN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2400_length_1230_cov_5.129362"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK614_10385"
     CDS             complement(<1..1074)
                     /locus_tag="PK614_10385"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRB82653.1"
                     /translation="MSRSSYKKVVLAYSGGLDTSVILKWLEEVYGCEVIAFCADLGQG
                     EDLKAIKKKAQSLGVKKVYVEDLREVFVKDHVFPMLRGNAIYEGSYLLGTSIARPLIA
                     RRQIEIAADEGAEAVCHGATGKGNDQVRFELTYMALHPQIKIIAPWREWTMRSRRELI
                     EYAEKHGIPVTATKAKPYSMDMNLFHTSYEGGILEDPWKAPPEEIFVMSVSPERAPNK
                     AREVEIDYVAGNPVAVDGKKMSPPALLAHLNKLGGEHGVGRVDLVENRYVGMKSRGVY
                     ETPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELIYYGYWYAPEREMLQVALDE
                     AQRDVTGTVRVKLYKGTCTVVGRK"
     gene            complement(1090..>1230)
                     /locus_tag="PK614_10390"
     CDS             complement(1090..>1230)
                     /locus_tag="PK614_10390"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRB82654.1"
                     /translation="AHRGEEISAEVLDGPQSVVFDQAENRLHMQKAILVRLLGKKPVR
                     ST"
BASE COUNT          243 a          410 c          309 g          268 t
ORIGIN      
        1 cttccgccca accaccgtgc aggtcccttt gtagagcttc actcgaacgg tgccagtgac
       61 atcccgctgg gcttcatcga gcgccacctg gagcatttcc cgctccggag cataccaata
      121 tccgtagtaa atgagctcgg catagcgggg gatcagacta tcccgcagat gcagcacttc
      181 ccggtccatc gtgagtgact ccagacctcg atgggccgca tgcagaatcg tgcctccggg
      241 cgtttcgtac actccgcgtg atttcatccc gacatagcgg ttttcgacaa gatcgacgcg
      301 accgacacca tgctcgccgc ctaatttatt gagatgggcc agcaaggcgg gcgggctcat
      361 cttcttgccg tccaccgcca ccggattgcc cgccacatag tcgatctcga cttcccgggc
      421 cttgttcggc gcacgctccg gcgagaccga catcacgaaa atctcttcag ggggggcctt
      481 ccagggatcc tccaaaatgc cgccctcgta actcgtgtgg aacaggttca tgtccatgct
      541 gtaaggtttc gccttggtgg ccgtgaccgg aatgccgtgc ttctccgcgt actcgatcaa
      601 ctcacgccgc gagcgcatgg tccattcccg ccagggcgca atgatcttga tctgcggatg
      661 caacgccata taggtcaatt cgaagcggac ctgatcgtta cccttgccgg tcgcgccgtg
      721 acagaccgcc tcggcgcctt catcggccgc aatttcaatt tggcgccgcg caatcaaggg
      781 acgggcgatc gaggtgccca gcaggtagct accttcatag atcgcattcc cgcgcaacat
      841 ggggaacaca tggtccttga cgaacacctc gcggagatcc tccacgtaga ctttcttcac
      901 gcccagggac tgcgccttct ttttgatggc cttcagatcc tcgccctgcc ccaggtcggc
      961 gcaaaacgcg atgacttcgc agccatagac ctcttccagc cacttcagaa tcaccgacgt
     1021 gtccaggccc cccgaatagg ccaataccac tttcttgtac gatgaccgac tcatacgact
     1081 ccctttcact tacgtgctcc gtacaggttt ctttccgagg agcctgacca gaatagcctt
     1141 ctgcatgtgc aatcggttct cggcttgatc gaataccacc gactgcggtc catccaacac
     1201 ctcggcactg atttcctcac cgcgatgagc
//
LOCUS       DAORQN010000336         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2514_length_1165_cov_4.184685, whole genome shotgun sequence.
ACCESSION   DAORQN010000336 DAORQN010000000
VERSION     DAORQN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_1165_cov_4.184685"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PK614_10395"
     CDS             complement(<1..612)
                     /locus_tag="PK614_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HRB82655.1"
                     /translation="MRWLRAAAALIAMALAIVGPYQPDATAQPSGRTYLVKLATDLPD
                     GDLDDGVCSTVDHTLSSDVPDPETCTLRAAIQNANLDSTKDTVKFAFSTLTTIKLKSY
                     LPVLQNPVEIDGWTQSPGESRIPEPAVRVDGVGLPRLSCGPIQMGSNAVAHGRAFVVL
                     GARSLIRGLNISGFPCDDIVVWNAPDTVISANFIGTNPEGSKVD"
BASE COUNT          301 a          285 c          301 g          278 t
ORIGIN      
        1 gtcgaccttt gatccctccg ggtttgttcc gataaagttt gcagaaatca cggtatccgg
       61 ggcgttccag accacaatgt catcgcacgg gaagccggaa atattgagac cgcggattag
      121 cgaccgcgct cctaatacga cgaaggctct cccatgtgcc acggcattcg agcccatctg
      181 gatgggtcca catgacaaac gtgggaggcc aactccgtca actcgaaccg cgggctcagg
      241 aattctcgat tcgccagggg actgagtcca tccatcaatc tcaaccgggt tttggagtac
      301 tggtagataa gatttgagct tgatagtcgt cagagtgcta aaggcgaact tgaccgtatc
      361 cttggttgag tcaagattcg cattttgaat ggcagcgcgg agcgtgcatg tttctggatc
      421 gggtacatct gatgacagag tgtgatcaac agtgctgcag actccgtcgt cgagatcgcc
      481 gtccgggaga tccgtagcga gtttcacaag gtaggttcgc ccgctaggtt gcgcagtagc
      541 gtcaggttgg tacgggccca ctatggcgag tgccatggct atgagcgccg ccgcagctct
      601 cagccatctc atacgcatcc ctgatgcgcc ccgctgcatg gagtgccgtc ctttatgttt
      661 tcgcctctac atgtttcggc cagaacaaat ggaggcgcca gagacaaact ggcgctcgaa
      721 gaaattgtct tactgtcagg tgcgaataat aaacagttaa tcccacgact ggccctgccg
      781 ttctgcagga agtcaaaacc aattcttatc aaccgaagac ggtcgaggaa acactgttga
      841 gaaatcggca aagcaggatt cattggtaga acccaattac tattgcttag tttctatatt
      901 ttcattggtg ttgcaagtat gaataccctc aaacactagc taaagaggat tgagagcttc
      961 tgggcagggg agagatgccc atgaataact aaaatgcacg tatttaacta ggctgaacgt
     1021 caaattctcg tagctagtat tactaatata caaaaaagtc aagggctagg tgcacttttt
     1081 ctcttaccac acggaggagc gcaaccagcg ccctcactca acctgcataa ctagatatag
     1141 actgggatat cttaatggat aaggg
//
LOCUS       DAORQN010000337         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2519_length_1162_cov_4.798555, whole genome shotgun sequence.
ACCESSION   DAORQN010000337 DAORQN010000000
VERSION     DAORQN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2519_length_1162_cov_4.798555"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1127
                     /locus_tag="PK614_10400"
     CDS             222..1127
                     /locus_tag="PK614_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRB82656.1"
                     /translation="MKRSARSITVGIGFLAALLASGMQPFSSLAAEGSLEQDAPVVVV
                     DAVEVKGKRIENVEDVKQELARRPGSNILIEEKQITESRALNLQDVLQFAPGVRFQSR
                     FGADEGQFQIRGTSLRNNFHHRGINILINGIFFGDADGFSDFESIDLLAYERIEVYKG
                     ANALRYGANSIGGAINFVPRTGYSASTLQMRMLGGSFGMVSGQVSSGKVLQPFQVGNM
                     SATMDYYISVSGNRQDGYQDNSQQARERINANIGLQLGNHQEIRAYFLQANVAERIPG
                     SLTTGQLFQNRQQAMEQRNVVLENL"
BASE COUNT          254 a          314 c          362 g          232 t
ORIGIN      
        1 ccagttttgt cgccttggcc ggcaacccca tcgaggattt ttcgcaggtg caggctattc
       61 gccggagatt gaagcaggga gtcttgctgg acgatcaaac ggtccagaac gctgcctcgt
      121 cagcagcgga tcatcagggg cactaatacg tgatgctcac aaccggcatc gcggcattat
      181 tcatggtcat atgtggtctg ggcagatgaa ggagagaggg aatgaagcgg agtgctcgta
      241 gtatcacagt gggaatcggc ttcctggcgg ccttgctggc gagcggtatg cagccgttct
      301 cctctctggc ggccgaaggg tcgttggagc aggatgcgcc ggtggtcgtg gtcgatgcgg
      361 ttgaggtaaa gggcaagagg atcgaaaacg tggaggatgt gaagcaggaa ctcgctcgcc
      421 gaccgggcag caatatcctg atcgaggaaa agcagatcac cgagtcacgc gcgctcaacc
      481 tgcaagacgt gttgcagttc gcccccggcg tacggtttca gtcgcgtttt ggcgccgatg
      541 aagggcagtt tcaaattcgt ggcacctcgt tgcgcaataa ctttcaccat cgcgggatca
      601 atattctcat caacggaatc ttcttcggcg atgccgacgg attttcagac ttcgaatcga
      661 tcgaccttct ggcctacgaa cgcatcgaag tctacaaggg cgcgaacgcc ctgcggtacg
      721 gcgcgaatag catcggtggc gcgatcaatt tcgtgccgcg caccgggtat agcgcctcca
      781 ccctgcaaat gcggatgctc ggcggcagct tcggcatggt gagcggccag gtgtccagcg
      841 gcaaggtctt gcagcccttc caggtgggca acatgagcgc gacgatggat tactacatca
      901 gcgtgtcagg caatcgccag gacggatatc aagacaacag tcagcaggcc cgggagcgaa
      961 tcaatgccaa catcggcctg cagctcggca accaccagga gatccgcgcc tactttctgc
     1021 aggcgaatgt ggcggagcgc attcctggat cgttgaccac gggacagctc tttcaaaacc
     1081 ggcagcaggc gatggagcaa cgcaatgttg tccttgaaaa cctgtgacaa tgatccatcc
     1141 ggcgccgttg cggatgcgcg gt
//
LOCUS       DAORQN010000338         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2550_length_1148_cov_5.197621, whole genome shotgun sequence.
ACCESSION   DAORQN010000338 DAORQN010000000
VERSION     DAORQN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_1148_cov_5.197621"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK614_10405"
     CDS             <1..667
                     /locus_tag="PK614_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRB82657.1"
                     /translation="PRSGWITMDPTPPAPENIGQTWWQSASRVMDSVRLQWDRLFVHF
                     SANDQLTVVQGLRESGEAVRTKLSESLNSLSAQSSAMFRRFMSSFTSTGIPETVLLII
                     LAIGATYAGRRLLRSSRNTAAPPDVLSAHQRTVVTLYTAMLHCLAQRGIVKSASATPM
                     EVLRHVREEWAEAWPSADALTHLYTRVRFGHAPLTPEDHTAAENLLRRLHTLERATTR
                     SQQ"
     gene            838..969
                     /locus_tag="PK614_10410"
     CDS             838..969
                     /locus_tag="PK614_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82658.1"
                     /translation="MVVENDMGKDRVEEKCELERETGFEPATLALAIQKIEILERLD"
BASE COUNT          276 a          341 c          301 g          230 t
ORIGIN      
        1 cccacggtca ggctggatta cgatggaccc aacgccgcct gctccagaga acattgggca
       61 aacctggtgg caatccgcca gtcgtgtgat ggattcggtc cggcttcaat gggatcgcct
      121 cttcgtccac ttcagcgcaa acgatcaact caccgtcgtg caaggccttc gggagagcgg
      181 cgaagccgtt cggacaaaac tctccgaatc tctcaactcc ctctcggccc agagctccgc
      241 gatgttccgg cgcttcatgt cctcgttcac atcgaccggc attccggaaa ctgtactctt
      301 gatcattctg gccatcggcg cgacgtatgc gggtcggcga ctgctgcggt cctcccgaaa
      361 cacagcggca ccgccggatg tgctgtcagc gcatcaacgc accgtcgtca cgctctacac
      421 cgccatgctg cactgccttg cgcaacgcgg catcgtgaag tcagcgagcg ccacccccat
      481 ggaggtgctt cgccatgttc gtgaagaatg ggctgaagcc tggccctccg ctgatgccct
      541 tacacacctc tatactcgag tccgatttgg tcacgctccc ctcactcccg aagaccatac
      601 cgctgccgaa aacctgctgc gcaggctcca cacactggaa cgagcgacaa ctcgttcaca
      661 acaatagtcc tgactgaggc cagatttcag cgactcgaca gagacaaacg cgtatgaaca
      721 ggagcgggga aaaacggcgt gctcggctgg caagtccgcc cgtcaacatg aaacctgtca
      781 gggcgaccta aatgatggac aaaggccggc cagggttcca gccgaaaggg acgaaccgtg
      841 gtggtcgaaa acgatatggg taaagatcgt gttgaggaga agtgtgaatt ggagcgggaa
      901 acgggatttg aacccgcgac cctcgccttg gcaattcaga aaatagaaat tctagaacgg
      961 cttgactgag ctatggcttc cccgcaaacc attgataatg gaacggctta taagattcca
     1021 atgactcaat agactctaac gcgaagagta tgattgtacg gtttctagca caaatctagc
     1081 acaccccggt agataccatt ctgtgcactt aatacttctc tggaccggta acgtctatcg
     1141 ataatggg
//
LOCUS       DAORQN010000339         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2578_length_1135_cov_4.212963, whole genome shotgun sequence.
ACCESSION   DAORQN010000339 DAORQN010000000
VERSION     DAORQN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2578_length_1135_cov_4.212963"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>1135
                     /locus_tag="PK614_10415"
     CDS             140..>1135
                     /locus_tag="PK614_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRB82659.1"
                     /translation="MSIYRRGVRVFWTQQLPGRILTQAMALLLVVLGSVSLSEAADSG
                     KLVEKDGKYVFVESMDPSLKLLLERSVKSGMIAQEEYDQVLKDSEERTQLLSPSFRGW
                     YDRGFNLSMNDNAFFLKIRFRSQLRFTQRFRNDAWREAKNFPELLGVFGDYRANRSEN
                     SASSFNVRRLRFYFMGHLFDPDFKYYIQMRAETAENAQSPGSLSLFDVNFTYTRLPWA
                     NVQFGQYKVYFNRSQINSTASMQFAERALVQDAFTASGLDRRDIGITIMNDEELFPVN
                     YYIGVFNGAGPSFNRLGSFTSEEATVGCPGGQTGGNPFPSPAGCPVNQRNLNANTR"
BASE COUNT          238 a          317 c          328 g          252 t
ORIGIN      
        1 caagcagact cgagcggtgg cgtatcggca gttcttgaaa tccggagagg ccggcacggc
       61 accaccgcgc tgacaaccgg ccggccttcg aactgaccga tccgtccatc gatcacaaca
      121 tgacatcatg gagttggtta tgagcatata caggcgaggt gtgcgcgtat tttggactca
      181 gcagcttccg ggccgaatcc tcactcaggc gatggctctg ctgctggttg tgttgggctc
      241 tgtgtccctg agcgaggcgg cagattcagg caagctggtg gagaaagacg gcaagtatgt
      301 ctttgttgaa agcatggacc cgtcgctcaa actcctgctc gagcgatccg tcaagagcgg
      361 catgattgcc caggaggagt acgaccaggt gctgaaagat tcggaggaac gcacccagtt
      421 gctgtcgccg agttttcgcg gttggtacga ccgtggattc aacctatcga tgaacgacaa
      481 tgccttcttc ctcaaaatcc gctttcgcag tcaacttcga tttacgcagc ggttccgcaa
      541 cgatgcctgg cgtgaggcca agaattttcc cgagttgctg ggggtattcg gcgattatcg
      601 agccaatcgt tcggagaaca gtgcgtcatc gttcaacgtg agacggttgc ggttctattt
      661 catgggccat ttgttcgatc cggactttaa atactacatc cagatgcggg cggagacggc
      721 cgagaacgcg cagtcgccag gatccctgtc gctcttcgac gtcaacttta cgtacacccg
      781 tctgccgtgg gcgaacgtgc agttcggcca atacaaggtc tacttcaatc ggtcgcagat
      841 caattccacg gcctcgatgc aatttgccga acgtgcgctc gtgcaggatg ccttcacggc
      901 gagcggcttg gatcgtcggg atatcggcat cacgatcatg aacgacgaag aattgtttcc
      961 cgtgaattac tatatcggcg tgttcaacgg cgcagggccg agtttcaacc gcctgggttc
     1021 cttcacatcg gaggaggcca ccgtcggttg tcccggcggc cagacgggcg gcaatccctt
     1081 tccctccccg gcaggctgtc ccgtcaatca acgcaatttg aacgcgaaca cgcgc
//
LOCUS       DAORQN010000340         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2608_length_1117_cov_10.338041, whole genome shotgun sequence.
ACCESSION   DAORQN010000340 DAORQN010000000
VERSION     DAORQN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_1117_cov_10.338041"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="dapB"
                     /locus_tag="PK614_10420"
     CDS             <1..634
                     /gene="dapB"
                     /locus_tag="PK614_10420"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRB82660.1"
                     /translation="IVDDLSSLMERGEVVVDFTTPAATLGHLKIVAQHRRGIVVGTTG
                     FSSSELDELRSVSKQIPCVFSPNMSVGVNVIYKVIAEMAKTLGEDYDIEVIEAHHRLK
                     KDAPSGTALKMAEVLARAVNRDLGQVGVYARKGLIGERKRGEIGIQTIRAGDIVGDHT
                     VMFGTMGERIELTHRASSRDTFARGALRAARWVVKQPPGLYDMLDVLSLK"
BASE COUNT          265 a          251 c          332 g          269 t
ORIGIN      
        1 gatcgtcgac gatctttcca gtttgatgga gcgtggggag gtcgtggtag attttacaac
       61 gcctgcggcg actcttgggc atttgaagat tgtggctcag caccgacgtg gcattgtggt
      121 cgggacaacg gggttttcat cgtcagagtt agatgagctc cgaagcgtga gcaagcagat
      181 cccttgcgtg ttttccccca atatgagcgt gggggttaat gtgatttaca aggtgatcgc
      241 cgaaatggcc aagacccttg gggaggacta cgatattgaa gtgatcgaag ctcaccatcg
      301 ccttaagaag gatgctccga gtggtaccgc gctcaaaatg gcggaggtcc tggctcgcgc
      361 agtcaatcga gaccttggtc aagtcggcgt ttatgcgcga aaaggactta tcggtgagcg
      421 gaagaggggc gaaatcggaa ttcagaccat tcgagccgga gacatcgtcg gagatcatac
      481 cgtgatgttt ggaacgatgg gcgaacgaat tgagctcact catcgtgcga gtagccggga
      541 cacatttgcc agaggagccc tccgtgccgc ccgctgggtt gtgaagcagc cacccggtct
      601 ctatgacatg ctggatgtat tgagcctcaa gtaaaaaatc cctccgaagc tacctctttt
      661 tcattccaag acacctggaa tgattttgtg ctcctgatat gagaattcgt ttgtcgctgg
      721 gatcactgtc tgaatatgta gaaacgtaaa atctggggga ttggatagaa aaactgggca
      781 gacctggcag ttagctcaac gcattggcta gtaggataaa ggggaggatt gctctgcgat
      841 gagaggggca tcaagtccag agtggacaaa aggccagagg aagttggggt aaaccaactt
      901 cggctcaacg catgtgagcg tcctgtgaag gattattatt gcgacgggtt aatgtcgtga
      961 cgtgatcggt ctcggttctg cgtcagctgc ttttcgtcgg ccacagttga ggcacgcaag
     1021 gactgtgatc gccaagccag cattcaggtc aaccgcccgt tccggcaaca ttgttccgcg
     1081 gcatttgtca catttatgca ttcctggctt ataacat
//
LOCUS       DAORQN010000341         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2631_length_1101_cov_5.896750, whole genome shotgun sequence.
ACCESSION   DAORQN010000341 DAORQN010000000
VERSION     DAORQN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2631_length_1101_cov_5.896750"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /gene="ligD"
                     /locus_tag="PK614_10425"
     CDS             <1..1055
                     /gene="ligD"
                     /locus_tag="PK614_10425"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HRB82661.1"
                     /translation="FQGLREDKDARSVVVERTSENASSEKPGQANDRAKRPGSKNRAA
                     QGDLIKASSAQATVVGGVTLSHPDRVLFPEQAVTKLALARYYEAVGEWILPHVKDRPL
                     TLVRCPEGYQKDCFYQKHANDQMPDVIGRVEVPTDQGSACYTIAHSLEALLGLVQIGV
                     LEIHTWGAKRDKLDRPDRMILDLDPGPAVDWSSVIEGAQLLRTLLRELNLTSFVKTTG
                     GKGLHVVVPLQRVHTWDEVKTFSKSLAEHLERLIPERFVATMSKAKRQGKIYVDYLRN
                     AQGATAVAAYSTRARPGAPVSVPLEWDELSIDLRSDSFTLVNLPDRLRRLPKDPWAEY
                     GATKQRLTKTMRLRLT"
BASE COUNT          244 a          306 c          333 g          218 t
ORIGIN      
        1 ccttccaagg cctgcgtgaa gacaaagacg ctcgctccgt cgttgtcgaa cgcacatcgg
       61 agaacgcttc gagtgagaag cccggacaag cgaacgatcg cgcgaagaga cctggctcaa
      121 agaaccgcgc agctcaaggg gatttaataa aggcttcttc tgctcaagcg accgtggtcg
      181 ggggagtaac cctgagtcat ccggaccgcg tgctgtttcc ggagcaagcc gtcaccaagc
      241 tggcgctcgc caggtactat gaggccgtcg gcgaatggat ccttccgcat gtgaaggacc
      301 gtccgttgac tcttgtacgt tgtcctgaag ggtatcaaaa agattgtttt tatcagaaac
      361 acgcgaatga tcagatgcct gatgtgatcg gaagagtgga ggttcctaca gatcaaggga
      421 gcgcctgcta tacgatcgcc cactcgctcg aggcgttgct gggattggtt cagatcggcg
      481 tgttggaaat tcatacctgg ggggcgaagc gggataaact ggatcgtccc gaccggatga
      541 tcctcgacct ggatcctggt cccgctgtgg actggtcgtc cgtcatcgaa ggagcccaac
      601 tacttcgcac gttgttgcgc gaattgaatc tgacctcctt cgtcaaaacc acgggcggga
      661 aaggtctgca tgtcgtcgtg ccgttgcagc gagtgcacac ctgggatgag gtcaagacgt
      721 tctcgaaatc tctcgcggaa catctcgaac gcctgattcc cgaacggttc gtggccacca
      781 tgtcgaaggc caagcgccag ggaaagatct atgtcgatta cctgcgcaat gcgcagggcg
      841 cgacggccgt ggccgcctat tccacgcgcg ccaggccggg cgcgccggtg tcagtgccgc
      901 tggaatggga cgaactctcg atcgacctgc ggtccgacag tttcacgctc gtcaatcttc
      961 cggatcgact ccgccgactg cccaaagacc cgtgggcaga gtacggtgcg acgaaacaaa
     1021 gactcacgaa aacaatgcgg cttagactca cctagtgcat gaggatcatg gccagggtgc
     1081 tttcaacagg gacctggggg g
//
LOCUS       DAORQN010000342         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2633_length_1101_cov_2.409178, whole genome shotgun sequence.
ACCESSION   DAORQN010000342 DAORQN010000000
VERSION     DAORQN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_1101_cov_2.409178"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK614_10430"
     CDS             complement(<1..421)
                     /locus_tag="PK614_10430"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRB82662.1"
                     /translation="MPSKKTMTKTASRSKVAKAPKAPATTELLLEIGTEELPYQFVAP
                     AMRALQQSAETLLKDLRLTYGTVRTMGTPRRLVLLIEGLARQQASAVKEAMGPSKSVA
                     FDQNGQPTRAAVGFAAGQGIPVEDLQVRQTPKGEYLFA"
     gene            complement(442..>1101)
                     /locus_tag="PK614_10435"
     CDS             complement(442..>1101)
                     /locus_tag="PK614_10435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRB82663.1"
                     /translation="IQSLYLESLKQLGIDPKKHDIRFVQDDWESPTLGAWGLGWEVRL
                     DGMEITQFTYFQEIGGIPLNPITGEITYGTERIAMYLQQVDNVYDLAWTDGVSYGDVH
                     HRSEVEFSRYNFEEGEVPMLMATFQAFEGECQRLLDKKLTLPAYDYCIKTSHMFNLLD
                     ARGAISVTERTSYIARVRALARRCAESYLADREAMGHPLIKQSAGTGKRAGAHSPVPT
                     K"
BASE COUNT          198 a          331 c          337 g          235 t
ORIGIN      
        1 ccgcaaagag atactcgccc ttgggcgtct gccgcacttg taagtcttca acgggaatac
       61 cctggcccgc ggcaaaaccc acggccgctc tggtgggttg cccgttttgg tcgaacgcca
      121 ccgacttgga tggccccatg gcttctttga ccgccgacgc ctgctgccgg gccagtcctt
      181 cgatcaacag caccagccgc cgcggagtgc ccatcgtacg gacggtgcca taggtgaggc
      241 gcagatcttt cagcagggtc tcggccgatt gctgcagcgc gcgcatggcc ggggcgacga
      301 actgataggg caactcttcc gttccgattt ccaacaggag ttccgtggtc gccggagcct
      361 tgggggcttt ggcaaccttg gagcgtgagg cagttttggt catggtcttt ttggatggca
      421 tcgtcaggta ctgcctgcgg actatttggt tggaacgggt gaatgagcgc cggcgcgctt
      481 gccggtccca gccgattgct tgatcaaggg atgtcccatc gcttcgcgat cggccagata
      541 ggactcggcg caacgacggg ccagcgcccg cacacgggcg atataggagg tccgttcggt
      601 gacgctgatc gccccgcggg catcgagcaa attgaacatg tgcgaggtct tgatgcagta
      661 gtcgtacgcc ggcaacgtca gcttcttgtc cagcaaccgc tggcactcgc cctcgaacgc
      721 ctggaaggtg gccatcagca tgggcacctc cccctcttcg aagttgtagc gggaaaactc
      781 cacttcgctg cggtgatgta catcgccgta gctcacaccg tcggtccagg ccaggtcata
      841 cacgttatcg acctgctgca ggtacatcgc aatacgctcg gtgccatagg taatttcgcc
      901 ggtgatggga ttcaagggaa tgccgccgat ttcctggaag taggtgaact gggtaatctc
      961 catgccgtcg agccggactt cccaccccaa tccccaggcg ccgagcgtgg gggactccca
     1021 atcgtcctgt acaaaccgga tgtcgtgttt cttcgggtcg atgcccagct gtttcaggct
     1081 ctccaggtag aggctttgga t
//
LOCUS       DAORQN010000343         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2706_length_1069_cov_3.547337, whole genome shotgun sequence.
ACCESSION   DAORQN010000343 DAORQN010000000
VERSION     DAORQN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_1069_cov_3.547337"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>480)
                     /gene="cydB"
                     /locus_tag="PK614_10440"
     CDS             complement(<1..>480)
                     /gene="cydB"
                     /locus_tag="PK614_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRB82664.1"
                     /translation="YVVLDGYDFGVGILSPFVARQESERRLVRETIGPVWTGNEVWVI
                     AGSALLFLAFPKAFASVFSGFYLALMIMLWLLMGRGLAFELREHVDHALWRTFWDTIF
                     FLANLLLAFVVGLVVGNLLRGVPLNASGYFFLPLWTDLSPGPLPGILDWVTLLTGVT"
     assembly_gap    481..490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<491..>1069)
                     /locus_tag="PK614_10445"
     CDS             complement(<491..>1069)
                     /locus_tag="PK614_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB82665.1"
                     /translation="VKGAAFEGLFRTERGADLVLIGQPNMETMTIDNPLVVPSALSLL
                     AYDDLYAKVKGLDAFPPREWPDHIALLYYAYHIMVGLGTLLLAVMGLALAWLWRGRLF
                     TARWLLWLVLLSAPFPYVATTAGWMTAELGRQPWLVYGLFRTEEGISPLVHSGNALFT
                     LLGFLGIYLGLGLLFVLLFVETLRHGPTEPVVG"
BASE COUNT          259 a          359 c          310 g          131 t
ORIGIN      
        1 gggtcactcc ggtcagcaga gtgacccaat ccagaatgcc gggtagcgga ccggggctga
       61 ggtcggtcca gagcggaagg aagaaatacc cgctcgcatt caaaggaacg cctcgcagca
      121 gattccccac caccagcccc accacgaagg ccagcagcag attggccagg aaaaagatgg
      181 tatcccagaa cgtgcgccac aacgcgtgat cgacatgctc tcgcagttca aacgccaacc
      241 ccctgcccat caggagccac agcatgatca tcaaggccaa gtagaacccg ctgaacaccg
      301 acgcaaaggc cttcggaaag gccaggaaca acagggcgct gccggcaatc acccacactt
      361 cgttcccggt ccacaccggt ccgatcgtct cccgcaccag gcggcgttcg ctctcctgcc
      421 gggccacgaa tggcgacagg atcccgaccc cgaagtcgta gccgtcgagc accacatagg
      481 nnnnnnnnnn ccccacaacc ggctccgtcg gcccgtgacg cagagtttcc acgaacaaga
      541 ggacgaacag cagccccaac ccgagataga tccccaggaa gccgagcaag gtgaagagcg
      601 cattgccgga atggaccaac ggcgagatgc cctcctccgt gcgaaagagc ccatacacca
      661 gccagggttg gcggccgagc tccgccgtca tccagccggc cgtcgtcgcg acatagggaa
      721 aaggagcgct caacagcacg agccagagca accagcgggc ggtgaacagc cggccacgcc
      781 acagccacgc cagggccaat cccatcaccg cgagcaggag cgtgccgagc ccgaccatga
      841 tgtggtacgc gtaatacagc agcgcaatgt ggtcaggcca ctcgcgcggg ggaaaggcat
      901 ccagcccctt caccttcgcg taaagatcgt cataggcgag caggctcagc gccgacggga
      961 caacgagcgg attatcgatc gtcatggttt ccatgttggg ctggccgatc agcacgaggt
     1021 cggcgccgcg ctcggtacga aagaggccct cgaaggcagc gcccttgac
//
LOCUS       DAORQN010000344         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2713_length_1065_cov_7.292079, whole genome shotgun sequence.
ACCESSION   DAORQN010000344 DAORQN010000000
VERSION     DAORQN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_1065_cov_7.292079"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PK614_10450"
     CDS             <1..232
                     /locus_tag="PK614_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB82666.1"
                     /translation="TESYTPGEVLSLTLLLPEQVPFTSSIEVLRLDPLPVPGGAYRLH
                     ARFVRMTTQNRELLIRYIVRFQRDHLQDHYSV"
     gene            368..>1065
                     /locus_tag="PK614_10455"
     CDS             368..>1065
                     /locus_tag="PK614_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB82667.1"
                     /translation="MRGLKWFLGLLVALATIGALYQIVGMVLDRKQHPPIGRLIDIGG
                     HRLHLYCIGQGSPTVVLEAAAPGWSLYWSTVQPEVAQVTRVCAYDRAGLGWSERGPLP
                     RTGRRLARELHQLLTRGGIPGPYILVGHSLGGFVTRLYREEHPLDVVGMVLVDAGHES
                     EMRQAEFRSFANAGKSMLPVIRAMTMLGIPRLMASYDQLPPLLTGQEEKAPTEIRPCC
                     CCWQGVGWPGLRFSA"
BASE COUNT          182 a          346 c          313 g          224 t
ORIGIN      
        1 cactgagagt tacacacccg gtgaagtcct ttccctcaca ctgctgctcc ccgagcaagt
       61 tcccttcaca tcttctatcg aggtactgcg gcttgatccc cttccggtcc caggcggtgc
      121 gtaccgcctc cacgcccgct tcgtcaggat gaccacccag aaccgggagc tgctgatcag
      181 atacatcgtg cgttttcagc gcgaccacct gcaggaccac tattccgtct gaagcgcaga
      241 gaaggcttgg cagcccccgc acacgggggc agagacgttc attgggagtg ccgctcgccc
      301 ggtcttgggc gacacttcga ctcgtcccgg tttgcttctt ccatccatga caggtgatca
      361 tgtccccatg cgtggactca aatggtttct cggcctgttg gttgcgctgg cgacaatcgg
      421 tgcgctgtat cagatcgtcg ggatggtgct cgatcggaaa cagcatccgc ccatcggccg
      481 gcttatcgac atcggcggtc atcgactcca tctctactgc atagggcagg gcagtccgac
      541 ggtcgtgctg gaagcagccg ctcccggctg gtcactctac tggagcacgg tgcaaccgga
      601 ggtggcacaa gtcacgcgcg tctgcgccta cgatcgcgct ggcctcggct ggagtgagcg
      661 tggcccgttg ccccgcaccg gccggcggct ggcgcgagag cttcatcagt tgctgacccg
      721 cgggggcatc cccggcccct acattctcgt gggccattcg ctcggcggct tcgtcacccg
      781 gctctaccgc gaagaacatc ccctggatgt cgtcggtatg gtgctggtcg atgcgggaca
      841 tgaatcggaa atgcgccagg ccgaatttcg atctttcgcc aacgccggca aatcgatgct
      901 ccccgtcatt cgcgccatga cgatgctagg cattccacgc ctgatggcct cgtatgacca
      961 actgccaccg ctgttgaccg gacaggaaga gaaggcaccc acggaaattc gaccatgctg
     1021 ctgctgctgg cagggagttg gttggcctgg tttgcgtttc tcagc
//
LOCUS       DAORQN010000345         1025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5368
            NODE_2809_length_1025_cov_9.583505, whole genome shotgun sequence.
ACCESSION   DAORQN010000345 DAORQN010000000
VERSION     DAORQN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867585
            Sequence Read Archive: SRR5722946
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:32:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,093
            CDSs (total)                      :: 2,057
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 4 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_1025_cov_9.583505"
                     /isolate="AMR_MDS_5368"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..119
                     /locus_tag="PK614_10460"
     CDS             3..119
                     /locus_tag="PK614_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRB82668.1"
                     /translation="MTEDELRKSEAEIQKLTDQYVQKIDDVMKKKEGEILEV"
     gene            146..>1025
                     /gene="alr"
                     /locus_tag="PK614_10465"
     CDS             146..>1025
                     /gene="alr"
                     /locus_tag="PK614_10465"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRB82669.1"
                     /translation="MYTPSQFPPTSVSVDLNALAQNLAHVRRLAPRSEVLAIVKANAY
                     GHGALELTRTLQQLAVHRFGVATVDEGIALRQAGIHDAILVMGPTVPAQFPDLIAHRL
                     TPVLYRPDMVQVFAATISPNAAPYSVHVKIETGMGRLGVLPQEVPDLVAMPAFRATLR
                     LEGLMTHLADADNTETTHTEKQLAEFQQTLDTLRQRGISCPLTHAANSAGIIKYPASL
                     YSLVRPGIMLYGYHTLPNAAAAPGLKPILTWKATIAHLHNIQPGDSVSYNRTFVATRR
                     SRIAVLPMGYADGYNRL"
BASE COUNT          221 a          354 c          262 g          188 t
ORIGIN      
        1 agctgaccga agatgaactg cgcaagtcgg aagccgaaat tcaaaaactc accgatcaat
       61 acgtccagaa aatcgacgac gtgatgaaga aaaaagaagg cgagatcctg gaagtctagc
      121 gccctattct gcccccacaa gctgcgtgta cacgccgtct cagttccccc caacctccgt
      181 ctcagtcgat ctgaatgccc tcgcgcagaa tctcgctcac gtgcgtcgtc ttgcgccgcg
      241 ttccgaggta ctggctatcg tcaaggcgaa tgcttatggc catggcgccc tcgaactcac
      301 acgcacgctg caacaactgg ccgtccatcg gttcggtgtt gcgacggtgg atgagggcat
      361 cgcgcttcgc caggcgggca tccacgatgc cattctagtg atgggcccga cggtaccggc
      421 acaatttccc gacctgatcg cacatcggct gacccccgtg ctgtaccggc ccgatatggt
      481 ccaagttttt gccgcaacca tttcgcccaa tgccgcgccc tactccgtgc atgtcaaaat
      541 cgagaccggc atgggtcgct tgggcgtcct ccctcaagag gtgcccgacc tcgtcgccat
      601 gccggccttt cgcgccacgc tacggctcga aggcctcatg actcacctgg ctgatgcgga
      661 caataccgag accacacaca cggagaaaca actcgcggag tttcagcaga cactcgacac
      721 gttgcgccaa cgtgggatct cctgtcccct gacacacgcc gccaacagcg ccgggatcat
      781 caagtatccc gcaagcctct attccctcgt gcgacccgga atcatgctgt atggctacca
      841 taccctgcct aacgcagccg ccgcgcctgg attgaaaccg atcctcacct ggaaagcgac
      901 catcgcacac ctgcacaaca tccagccggg cgacagcgtc agctacaacc ggaccttcgt
      961 cgccacgcgg cgatcgcgga tcgcggtcct tccgatgggc tatgcggacg gctacaatcg
     1021 cctcc
//
